LOCUS       DAOQLJ010000001        45819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_220_length_45819_cov_6.266388, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000001 DAOQLJ010000000
VERSION     DAOQLJ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 45819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45819
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220_length_45819_cov_6.266388"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /gene="gcvT"
                     /locus_tag="PK522_00005"
     CDS             complement(<1..850)
                     /gene="gcvT"
                     /locus_tag="PK522_00005"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNC40143.1"
                     /translation="MLKMTPLNQAHRDMNAKMVDFGGWDMPLHYGSQLDEHHKVRRDA
                     GMFDVSHMLVVDIEGSDVRNFLKRLVANNVDKLTLPGKALYTCMLTPQGGIIDDLIIY
                     FLTESWFRLVVNAGTADKDIAWIMQQRDSLAPQLQIKPRRDLAMIAVQGPNARAKVWQ
                     VLPGAQAVTENLKQFQSATFENYFIARTGYTGEDGFEIVLPAQDAVDFWKKLHAAGVA
                     PAGLGARDTLRLEAGMNLYGQDMDETTHPLESGLAWTVDLKSERDFIGKQALLNAPNN
                     RQLVGLL"
     regulatory      complement(895..1002)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1460..1678)
                     /locus_tag="PK522_00010"
     CDS             complement(1460..1678)
                     /locus_tag="PK522_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40144.1"
                     /translation="MALMNQQTAMPTISSALTQGKDSQVGRVATQTKIGCTQREKASL
                     ASAEANPAEASFTRMAKVGSIYCGDSMT"
     gene            complement(1688..1834)
                     /locus_tag="PK522_00015"
     CDS             complement(1688..1834)
                     /locus_tag="PK522_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40145.1"
                     /translation="MGPTENTAATMQRPKAVTCTTTPVYMRVRTQTHVPGVGVCPERH
                     VATA"
     gene            2370..2588
                     /locus_tag="PK522_00020"
     CDS             2370..2588
                     /locus_tag="PK522_00020"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNC40146.1"
                     /translation="MEASKTSTKKSKYQTSENNGEVSIKEDRQLDWHNRIATAAYYKA
                     EARSFEPGHDIEDWLAAEAEEKDEQLTC"
     gene            2569..2967
                     /locus_tag="PK522_00025"
     CDS             2569..2967
                     /locus_tag="PK522_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC40147.1"
                     /translation="MNNLPVETRSTAEVRESISKQSDKYAALILSEDGIIQDCSKEGA
                     RLLGYMPENLIKLHITKLLPKLLEIDLLKAHQINPYLRFLSRIGHRFELIEMNGDRMI
                     GQLFFSDLENEGGHQLVVLINPIPEKKQLH"
     gene            complement(3257..3913)
                     /locus_tag="PK522_00030"
     CDS             complement(3257..3913)
                     /locus_tag="PK522_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40148.1"
                     /translation="MPKIKISFEIILPVLLIILLLVPYTLNRIGHSMYGIKQFPPVIL
                     MKLPVDHSAVNLPQAVKELQSFNVALELDTSELIKRTNDIIGKSSHGTGLQGLTGQVL
                     PGMQAEMAGANFQIDNPGPHVELFSSNRQTLWTWTVIPKKTGRQSLSLLLHLETMNGG
                     QQAKKVVELAEIQMYVENNPEEWLKQNAWWMILISIALGYVLVKYRSDKKHKQPEEPK
                     "
     gene            4013..4792
                     /locus_tag="PK522_00035"
     CDS             4013..4792
                     /locus_tag="PK522_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNC40149.1"
                     /translation="MNSNPTIPILVAHRGYPACYPENTLVGYEVALQAGACYVELDIQ
                     LSARHTPFLCHDDNLKRLTGIDQNITELADEIIKTLSIAYPQSTIADEATVSPTKTAP
                     FADLLSFCLLLEKWPQAKAFVEIKSESIVKFGLELTVETILQVLQPYREQCIIISFDW
                     QAVALARKKGMQQIGWVIPRWDDAHAKIADTLAPDYLFCSIWRIPPSIEERWKGPWQW
                     VVYTINDSQTALQYAQQNIGMIETDAIGVMLSDPTLKQHAC"
     gene            4782..5978
                     /locus_tag="PK522_00040"
     CDS             4782..5978
                     /locus_tag="PK522_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC40150.1"
                     /translation="MLASQSYDLVVIGGGIQGTAIAQAAAANGHHVLVLEKTGLAAGT
                     SSRSSKLIHGGLRYLEQGHFGLVRESLRERSQLLKLAPDLVKLSPFYIPIYQSTSRGK
                     WTIRAGLTLYGLLAGLGPASRFRSVPRADWAKLDGLSTAGLSHVFQYWDAQTDDRLLT
                     QALMHSAMKLGAELCCPAEFMQANLTQDRCELTYRWQNQSHHCTAAVIINAAGPWVNQ
                     ILHNVTPSPPTLPIELVQGTHLVLEGKPGSGCYYLESPVDQRAIFVLPWYEHTLLGTT
                     ETIFTGLPEACHPLAEEEAYLIECFRHYFPNRKVVVREKFSGLRVLPKAQGSAFSRSR
                     DIQLLCDNVSQPRIITLYGGKLTVSRATAQKVLTMAASSLPQRHPKANLAELPIKLPP
                     SASEYV"
     gene            5971..7422
                     /locus_tag="PK522_00045"
     CDS             5971..7422
                     /locus_tag="PK522_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNC40151.1"
                     /translation="MSNQAVTLAIDQGTHLSRALLFDPGGEVIAQFSEPVEIFHRSSV
                     EVEQDPDEIIDSIHSIVSQSLQFAAQHQKRISHIGLATQRSSVVAWHKTTGKALSPVL
                     SWQDRRAADWLMSFAAHQATIIERTGLRLSPHYGVSKLNWLRKNIPAVIAAEAQQQLA
                     FGPLASFILFHLLEGNPLVVDHANAARTLLWNITSCDWDDVLLKLFEIPRPYLPQCYP
                     IRHCYGYLKNHSIPITAVNGDQTAALCAAAEVSQEGVWINMGTGAFILKSTGCQLIRH
                     RKLLSGIADSDAQEKRYVLEGTVNNAGSAIDWLSEHESMPHPYQKLDAALRSITDPPL
                     FMNTISGLGSPWWGKGPAPQFHDLDGKPLTNLPADHRLVAVVESILFLIQANLDCMAE
                     THLPIKGITISGGLAQSDALCQKLADLSGLTVQRPALVEATAQGIAWLAAGKPSGWHK
                     QGKIDCFSSKATTARQNVINERYQKFVSILQSN"
     gene            7456..7857
                     /locus_tag="PK522_00050"
     CDS             7456..7857
                     /locus_tag="PK522_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC40152.1"
                     /translation="MAITGMNHFTVLTTDLDKSKAFYVSLLGLTEGYRPPFEFPGAWL
                     YCGDQAILHIMAGRPIPATIHGIIDHIAFSACNLQSVIDRLNQHNIAYTLNRLKGLES
                     WQLFCADPDGAKVELDFAASEPEPQNVKQTK"
     gene            complement(7877..10624)
                     /locus_tag="PK522_00055"
     CDS             complement(7877..10624)
                     /locus_tag="PK522_00055"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNC40153.1"
                     /translation="MWRYLLIITTFIISLSIMSLGHAQQPMQSLPANLTQITSVEGIT
                     EYQMPNGLKVLLLPDDSLDTVTVNIVYLVGSRDEGYGEAGMAHLLEHLLFKGTERHPD
                     IKNEFTKRGARWNGTTSYDRTNYYEVLTASTENLDWAISLEADRMINSRIEKADLDSE
                     MTVVRNEFEAGENNPFGVLSERMASTAFVWHNYGRAIIGSRSDIENVPIERLQDFYRH
                     YYQPDNAVLVIAGKFDETAALNSISKHFGAIPHPKRQLRKTYTVEPTQDGERNVMLRR
                     VGDTQIAATLYHIPAAAHPESAAIDLLVSIIKQVPGGRLHKSLVESKLATAIFGFDRQ
                     LRETGFVYFGANLLPTMSLDHASNTLISTIETLAAHPITAEELERARTRLLNKIEQLL
                     TNTRNLATTITEFIAMGDWRLLFLHRDRIQQVTLEDVQRVANTYFKPSNRTLGLFIPT
                     KTPDRAEIPAVTDLALMFKEYRGNAMLSLGEAFEATPENIEAKLIRTQLPGGMKLALL
                     PKKTRGNRVVVAFNLHWGDEQSKMNRATACGMTSAMLSRGTQRYTREQLRDAFDRLKV
                     HIDMTADGIAFETTGDHLTDALQLVAEMFRNPIFPEKEFEQLKLAILSSIENQKSEPA
                     AQAGIQLERHLMPFASAHWLYTPTIEERIERINQVSLDDVKRCHQDFYGSTDAELAIV
                     GDFEPKAITETLQQSLGDWKSPKPYQRISTPYQDIKAIDLNLETPDKANAVYQAGMNL
                     KIRDDHPDFPALVLGNYLFGGSADSRLWRRVREQEGLSYSVISSLTASAFEARGDFKI
                     FAIYAPQNRDRVEQAVDDEFTRVLKDGFTAEEVELAKAGYLEARRIARGQDKALAERL
                     VNYLHRNRTLQWDIAFEKNIAALTPELILTALRRHFDMTRLTQVKAGDFANTLSAKSP
                     Q"
     gene            complement(10714..11610)
                     /locus_tag="PK522_00060"
     CDS             complement(10714..11610)
                     /locus_tag="PK522_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNC40154.1"
                     /translation="MPTPPYLQKLVIIGVGLIGGSFALALRNAGLVKQIVGLGRGQEN
                     MLRARELGIIDEIAITPAAALAEADFVMLSTPVGQIQRVMAQIEPYLNKQTIVSDVGS
                     TKQNVIEAAQQQLTNHLAHFIPAHPIAGTEFRGAEAADPLLFKQKHLIITPLKENNEQ
                     AINLVKVLWESCGAKVSIMSAEQHDQMLAIVSHLPHMLAFTLMNHIQTCTDFDPNYVL
                     SYAGSSLRDCTRIAASSPEMWRDICLANRVQLLAELDKYQQALTELREQLANRNGEAL
                     LSLFKKASDLKLQWSNARDEKD"
     gene            complement(11652..12770)
                     /gene="hisC"
                     /locus_tag="PK522_00065"
     CDS             complement(11652..12770)
                     /gene="hisC"
                     /locus_tag="PK522_00065"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNC40155.1"
                     /translation="MKLFEFAPQYIRAIHPYQPGKPISELVRDLGLEKDSVIKLASNE
                     NPLGTSPLALEAMSVALNEIARYPDGSGYELKNALAEHYAVSVDQIVLGNGSNDVLEL
                     AARIFLKPGTAAVYSQHAFAIYPLLAQALGAQGISVPARRYGHDLVAMLNAITPETRI
                     IFIANPNNPTGTLCDPNELQRFVERVPQNVLVILDEAYDEYLPEANKANSIGWLKKFP
                     NLLITRTFSKAYGLAGIRVGFAFTHPELADLMNRIRQPFNVNSLGLIGALAALKDTEF
                     VRRAYELNRAGMAEITNGLRQLGVEFIPSYGNFITLRIRGDEINTAKAYQNLLKRGVI
                     VRPLGVYEMPHHLRVTIGLKSENEKFLSALEQVISNSA"
     gene            complement(12781..13851)
                     /gene="pheA"
                     /locus_tag="PK522_00070"
     CDS             complement(12781..13851)
                     /gene="pheA"
                     /locus_tag="PK522_00070"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNC40156.1"
                     /translation="MAEQLNQLRAKIDAIDNELLELMNARAKLAQAVGHQKGNAVTYR
                     PEREAQVLSRLQQLNAGPLSNERITALFTEIISACRAMEAPLTIAYLGPPGTFSEEAV
                     SKRFGHAVVTIACHSIDDVFRQVESGSAHYGVVPVENSTEGAVGRTMDLLVQTPLVVC
                     GEIELPVHHCLMAKHSDLTQIQKIYSHPQSFGQCIGWLNHQSAQLKSVAQINVASNAD
                     AARLAASDPHAAAIASSKAAEIFELTICARNIEDDPNNTTRFLVMGSQLVPVSGKDKT
                     SLAISTHDRPGAVYELLAPLAKYQVSMSRFESRPSRKGLWEYMFFIDLEGHQDNNNIA
                     SAIGELRDKATFLKILGSYPAA"
     gene            complement(13891..15081)
                     /locus_tag="PK522_00075"
     CDS             complement(13891..15081)
                     /locus_tag="PK522_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNC40157.1"
                     /translation="MAKKAQQVFKILTLNQISSVGLKQFPADQYLIGHDLVDPDVILV
                     RSHNMLDMDIPKHVIAIGRAGAGTNNIPVDAMSQRGIPVFNTPGANANAVKELVLAAM
                     LIASRNLVSAFQFVEGLQGDNQKLNELVEANKKHFSGIELPGRTLGIVGLGKIGRLVA
                     DAALKLGMKVMGYDPKISVDAAWSLSAEVQRAQSIEDLLRHSDFISLHVPLADSTRHL
                     INEQLIHYFKREVILLNFSRDEIVSEAAVLAGIKSGKIKYYVCDFPSQHLQNEKGVIT
                     LPHLGASTREAEENCAVMVVKQTIDHLQNGNIKNTVNFPDVIMERESPYRVAVANANV
                     PNLLGQISTCMATAGLNIHNMTNKSRGEMAYTLVDVDSPVSKQVIKAISLITGVLKVR
                     YLPV"
     gene            complement(15074..16177)
                     /gene="serC"
                     /locus_tag="PK522_00080"
     CDS             complement(15074..16177)
                     /gene="serC"
                     /locus_tag="PK522_00080"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNC40158.1"
                     /translation="MNKVYNFSAGPAVLPQEVLEQARDEMLDWHESGMSVMEMSHRGE
                     EFMSIIAKVEADLRDLVKIPNHYKILFLQGGASSQFAMVPLNLLQTKKEADYVNTGQW
                     SQKAIEEASKYGTVNIAASSVGNHFTGIPEIDTWKMSRNAAYLHYTSNETIGGVEFNW
                     IPDLSKIAGGSTDIPLVADMSSNFLSRPVDISRFGLIYAGAQKNIGPAGLVLVIIRED
                     LVQAPTAGTPTLFSYQAHVQHDSMYNTPPTYAIYMMGLVLEWLKKQGGVPAIEALNLA
                     KSKLLYDLIDASHLYQCPVALQDRSRMNVPFILRQSALDIEFLKQAKARGLVQLKGHR
                     SVGGMRASIYNAMPYEGVKALVTFMKEFENQYG"
     gene            complement(16214..18760)
                     /gene="gyrA"
                     /locus_tag="PK522_00085"
     CDS             complement(16214..18760)
                     /gene="gyrA"
                     /locus_tag="PK522_00085"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNC40159.1"
                     /translation="MNQFAKETLTISLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMHELSNDWNRPYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQNFSLRYVL
                     IDGQGNFGSVDGDNAAAMRYTEIRMAKIAHELLADLDKETVDFGPNYDGSEREPLIFP
                     AKIPNLLINGSSGIAVGMATNIPPHNLSEVIDACLRLLNNPETSIDEIIECIQAPDFP
                     TAGIIYGTSGIRDGYLTGRGRVVMRARTHFEDLEKGSRQSIIIDELPYQVNKANLLIR
                     IGELVRDKKIEGISDLRDESDKSGMRVVIELKRGEIPEVILNNLYKETQMQDTFGINM
                     VALIDGQPRLLNLKQLLQAFLRHRREVVTRRTVYELKKARERGHLLEGLAVALSNVDE
                     IIALIKAAATPAEAKKALIGKAWSSQLVAEMLARSAQDTAAFRPIGLGVEFGLFDHGY
                     QLSDVQAQAILDLRLQRLTGLEQQKIVNEYKEVIDQIIDLLDILANADRITAIIVDEL
                     RSIKDQFGDQRRSEIVTNTQELNTEDLITPADVVITLSHAGYIKSQLLDDYRAQKRGG
                     RGKLASATKEDDFIDNMFMANTHDYILCFSSLGRVYWVKVYNVPQGGRVSKGKPINNL
                     LQLEKAEKINAVLPVKAFDDNRYIFMATSLGTVKKTPLSEFSRPRSNGIIAIGLDEGD
                     YLIGVALTEGNHDVMLFSDGGKAMRFSENDVRPVGRSARGVRGMRLGAGQKVISLLVA
                     ENEELTVLTATENGYGKRTPISEYTRHNRGTQGMIAINTSARNGKVVAAQLVKQDDEI
                     MLITTGGILIRTRVSEIREMGRATQGVTLINLDEGEKLVGLERVVEADEE"
     gene            19158..20354
                     /locus_tag="PK522_00090"
     CDS             19158..20354
                     /locus_tag="PK522_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNC40160.1"
                     /translation="MSYLMNTYQRLPVTFVKGSGVWLWDNDGNRYLDALSGIAVCGLG
                     HCHPALAKALSHQAETLIHTSNLYHIENQELLAERLALLSGMDKVFFCNSGAEANEAA
                     IKLARLYGHHRGIELPTIIVMEKSFHGRTMATLTATGNRKAQAGFEPLLTGFVRVPYD
                     DLVAVEQIAANNKNIVAILVETYQGEGGVNFPQVNYLDGLRRLCNQNNWLLMLDEVQC
                     GIGRTGKWFAFQHSNSSPDVITLAKGLGSGVPIGACIAKGPAAEIFKPGNHASTFGGN
                     PLVCKAALTTLEVIEQDHLMQNAVSMGDLMRNRFHEKLANWSNVIKIRGQGLMLGIEL
                     PIACGELVKEALAAKLLINVTSERVVRLLPALVITQVEAEQIVDKTSSLIDRFLTTQY
                     NNTTPT"
     gene            20382..21296
                     /gene="argF"
                     /locus_tag="PK522_00095"
     CDS             20382..21296
                     /gene="argF"
                     /locus_tag="PK522_00095"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNC40161.1"
                     /translation="MTPKHFLKINDFTLDEFEYLFERAHWIKNEFKQYRKYWPLTDRT
                     LAMIFDKHSTRTRLSFEAGMCQLGGSVIYLSTRDTQLGRGEPVEDAARVISRMVDIIM
                     IRTFEHSIIERFANHSRVPVINGLTDEYHPCQIIGDIFTFIEHRGSIKGKTIAWIGDS
                     NNVCNTWLQAAEVFDFNVHVSTPPGYEVEPERAGLYGTNHYEEFSSPHDAVKNADLVT
                     TDVWTSMGFEDETEIRKNAFADFCVDDEMMAYAKADALFMHCLPAHRGEEVTNEVLEG
                     RQSVVWDEAENRLHVQKALMEYLLLGKI"
     gene            21348..22568
                     /locus_tag="PK522_00100"
     CDS             21348..22568
                     /locus_tag="PK522_00100"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNC40162.1"
                     /translation="MKKVKKVVLAFSGGLDTSVILKWLQEVYQCEVVTFTADIGQGEE
                     IEPARAKAQRLGIKEIFIEDLREEFVREYVFPMFRANTIYEGEYLLGTSIARPLIAKR
                     QIEIARETGADAVSHGATGKGNDQVRFELGYYALQPDIQVIAPWREWDLTSREKLLSY
                     AERHGISVEMKQKGGSPYSMDANLLHISYEGRSLENPALEPEESMWRWTQSPEKAPNE
                     SEYIELEYERGDIVAINGKRLTPATILEQLNQLGGKHGIGRLDLVENRYVGMKSRGCY
                     ETPGGTIMLRAHRAIESITLDREVAHLKDELMPRYASLIYNGYWWSPERMALQTMIDE
                     SQVHVNGWVRVKLYKGNVIIVGRDSKTDSLFDTNIATFEDDAGAYNQADAAGFIRLNA
                     LRLRIASKIRAPRK"
     gene            complement(22611..23426)
                     /locus_tag="PK522_00105"
     CDS             complement(22611..23426)
                     /locus_tag="PK522_00105"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNC40163.1"
                     /translation="MHPMLTIAVKAARRAGNIINQASRNLDILNVTQKSHSDFVSEVD
                     GAAEEAIIKILLDAYPNHAILAEESGSQSGQKQSDYQWIIDPLDGTTNFLHGFPKYCV
                     SIALLHRGVLSQAVIYDPVSNELFTASRGRGAYLNDHRIRVSKRTNLSESLIGTGFPF
                     RDLSFMEAYLAMFKAVIPKTAGIRRPGSAALDLAYVAAGRYDGFWEMGLSPWDIAAGC
                     LLIIEAGGMVSDLEGNDHYLKSGQVVAGNPKIFPQLMEIILPYITDQIIAQNR"
     gene            23466..24200
                     /locus_tag="PK522_00110"
     CDS             23466..24200
                     /locus_tag="PK522_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNC40164.1"
                     /translation="MIHHSPLDNVRVVLSHTSHPGNIGASARAMKTMGLSSLYLVNPK
                     SFPDNEAYVRATNARDILDQAVICSTLDIALQDCVLAVAMTARARELTHQVIDTRQIA
                     QKLVEIATESPVALVFGRESSGLTTAEVTKCQLTVHIPTSSTYASLNLAAAVQIMCYE
                     LRMGAVTPESTSPPPEVPQLASLNEIELFYQHLEEALITSKFLDPQQPKRLMQRIRRL
                     FSRTALEKEEVNILRGIIHALAKEKR"
     gene            24305..25048
                     /locus_tag="PK522_00115"
     CDS             24305..25048
                     /locus_tag="PK522_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HNC40165.1"
                     /translation="MTTNTIFKHKVSAIINNKHLLKHPFYMAWTEGKLTQEQLRHYAE
                     QYFYNVLAEPTYLSAVHFNTPHGHTEENCGDISVRQEVLKNLIDEEHGSGNHPALWKA
                     FAFALGANDKSLANAAALPETDNLVSTFRDICLNSPFYVGLAALHAFESQVPDIAAVK
                     IEGLAKFYGMNNPKDYEFFSVHQKADIYHSEAEWSIIERFADTPEKQAAVLAATQRAC
                     DALWKFLDGIHENYCANLICDQQTAVTLH"
     gene            complement(25109..27340)
                     /locus_tag="PK522_00120"
     CDS             complement(25109..27340)
                     /locus_tag="PK522_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNC40166.1"
                     /translation="MIQRCNNVLIRLIGMVFALALVLSISNVLATNQFNQPKPVVVAD
                     SSAIPAITPNPSNTVKFETITVETSAASELTAEPIQKLKGNELRLRTESTLGRMLERE
                     LGVSNLSYGPGVGQPVIRGMSGPRVRVMQNGIGMHDLSAISPDLAIPFDPILANGITV
                     IKGPATLQYGANAIGGVVDISHQRIPDRIPTNGMSGRTDFRFDHNPGEKAGMISLDIG
                     RSMVALHFDYFHRSANNMHIPGSALDESAIRQQFQVEPTTNSNNVIQNSHSHSQGGSA
                     GISLVGKYGYLGGSYHETARNYGIPPGVPGHGDSSRSEQEAIRIDMNQRRYDLEGFWK
                     TYWGALPSIKAKLSYIDYDHKDFDKNISARNSTLTRFGNSVWESRLEMNHQKGDWLDG
                     KFGMQWQNRNFSATGLETFTPSSNVDSIGFFLTETLVVNDRTTFELGGRIEHQTTSPK
                     SNELRLAGIPSPLPLPNSLNYLTYSLAASLNYEILPLTSFFLNWQRAQRSPDVQELFA
                     SGPNFATRSYQLGRMDLNTEQTDHYELGLRYAGNVISFNANGYYKSVEDFIYLKNQGF
                     FFNFAPDPPRFQLACANLRNCLPIFGYQADTANFWGYEAEITMRPKIAATYNPRFTFF
                     SDYVRGSFQSGSLGDVPRLPPLRFGAEFGLQWMQWRSLLRYTHALEQNRPGLLETETP
                     GYNRLDLDITYDWKASDRMKILFFAKLSNMTNATIRNSTSFLRSFAPEAGFSSQVGFS
                     AVF"
     gene            complement(27337..28221)
                     /locus_tag="PK522_00125"
     CDS             complement(27337..28221)
                     /locus_tag="PK522_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40167.1"
                     /translation="MIKKFNCAVLVILLISFGINAVAHEGEPNMGFAWREGVIEIDIR
                     KQGTKLGDLSAFVIRFTDSIAPYRMGDAGFTGIEFDQGGILGYQAETHLMKWSSEQSQ
                     WLTEGFSEQIIISRLSDEKRISASEGKGIQGFIENLTNTSTFESHPIFKIQKPDGQVP
                     EDGAYMVIITVLGVDATGENILYQPSQPFALVFHINAKGNFNGLALSEALDVSPVLKL
                     NNYSRIDALFNWAEENYSRYFPHAVKSRFIEGYYARCYNNGTCLGSLDGKIYTLGGEL
                     GGLTDHGAIETFYQAAGL"
     gene            28378..29361
                     /locus_tag="PK522_00130"
     CDS             28378..29361
                     /locus_tag="PK522_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40168.1"
                     /translation="MSPYRLIFAAVISLLISLPSFAGELHQHIEVNTTNQRVQVNFCR
                     PERGCSLVKPETIGLPPGQLPLDKLTGTAIYVSEFTALPNGIAIDSPGFEGIAGDLSA
                     GDRVHYVASSRLSFWNPAERQWSLAPEGIRIRLAGGLDLQPDQSCGQVFCLPKAVEGF
                     TIFNRQGVSGINSLIVGEARPDGSLHTHLDWILETEQGAPNGPIGAYLVELSIRTDAY
                     PTPSAPFWIMFNHSLPITEFQQAVADRVLKNSIDTALADKLFDWAESNYLSLFPNKTT
                     SFIAVGYYARCYQNGTCVGVRDNYVFAVGGEFGPNVVNLGEFSSLLKLAGF"
     gene            complement(29521..30636)
                     /gene="dnaJ"
                     /locus_tag="PK522_00135"
     CDS             complement(29521..30636)
                     /gene="dnaJ"
                     /locus_tag="PK522_00135"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNC40169.1"
                     /translation="MAKRDYYEVLGISRDADENEIKKAYRKLAMKHHPDRNAGDTKAE
                     EKFKEAKEAYEILSDASKRAAYDQYGHAGVDTSAAGAGHGQGFGDAFSDIFGDIFGMR
                     GGGGRSNVHRGADLRYNLEITLEQAARGSETKIRIPTMTTCDTCNGSGAKPGTSPKTC
                     STCDGHGQVRMQQGFFSIQQTCPKCHGSGKMITSPCSSCHGAGRIKQHKTLNVKIPIG
                     VDDGDRIRLSGEGEAGINGGPAGDLYVVIQLSSHPVFQREGNDLHCEIPISFATAALG
                     GEIEVPTLEGHAKIKISAETQTGKIFRLRGKGIKGVRSNSAGDLLCHVAVETPVNLTA
                     RQKELLQELEDISQKDNSRHSPRAKSWMEKAREFFSE"
     gene            complement(30832..32763)
                     /gene="dnaK"
                     /locus_tag="PK522_00140"
     CDS             complement(30832..32763)
                     /gene="dnaK"
                     /locus_tag="PK522_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNC40170.1"
                     /translation="MAKIIGIDLGTTNSCVAVMENGKPKVIENSEGTRTTPSIVAYTD
                     DNEILVGASAKRQAVTNPKNTLFAVKRLIGRRFDEDMVQRDIKMVPYSIVRADNNDAW
                     VEVRGRKIAPPEISAQVLIKMKKTAEDYLGEPVSEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGMDKKEGDRKIAVYDLGGGTFDISIIEIAEIEGEHQFEV
                     LATNGDTFLGGEDFDSRVIEYLVDEFKKESGIDLKKDMLALQRLKDAAEKAKIELSSS
                     QQTEVNLPYITADASGPKHLAIKITRAKLESLVEELIERTIEPCRIAIKDAGLSFSDI
                     HDVILVGGQTRMPKVQEKVKEIFGKEPRKDVNPDEAVAIGAAIQGGVLQGDVKDVLLL
                     DVTPLSLGIETLGGVMTKLIQKNTTIPTKANQVFSTADDSQTAVTIHVLQGEREMASG
                     NKSLGQFNLTDIPPAPRGMPQIEVTFDIDANGILHVSAKDKATGKENKIKIQASSGLS
                     EDEIQRMVKDAETHAEEDKKALELVNSRNQCDAMIHSVRKSLGEYGDKLEADEKAKIE
                     AALKEAEEALKSNDKDIIDAKTQALTQASQKLAEKIYAQDQTQSEQPKTESTAQQGGE
                     KTVEGEVVDAEFEEVKTKK"
     gene            complement(32838..33419)
                     /gene="grpE"
                     /locus_tag="PK522_00145"
     CDS             complement(32838..33419)
                     /gene="grpE"
                     /locus_tag="PK522_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNC40171.1"
                     /translation="MQESHNSSSSEQELISVGTETSSASNTGNTVSDSVIESTPSLEQ
                     LLRDAELKAAEHHDAWLRAKADAENIRKRAQADIASAHKYAIEKFSVELLAVMDSLDA
                     ALAVENVTLENFRNGVELTHKQLTNVFEKFNIKAISPVGEKFDPHQHQAMCMVESELP
                     ANTVTQVMQRGYLLFDRVIRPAMVAVSKNKEGT"
     gene            complement(33499..34881)
                     /gene="purB"
                     /locus_tag="PK522_00150"
     CDS             complement(33499..34881)
                     /gene="purB"
                     /locus_tag="PK522_00150"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNC40172.1"
                     /translation="MTFQTICALSPLDGRYHAKTSALRPYFSEFALIRYRVIVEIEWL
                     KALSNESLIGEVSPFSANTVGKLENLIKNFSVTDAEAIKAIEASTNHDVKAVEYWLKE
                     TFSQYSDIANVIEFIHFACTSEDINNLSHGLMLKNSRDEVMLPMLHALIDKLQTMAHQ
                     FASVPLLSRTHGQPATPTTMGKEMANVAYRLNYACDRLRAIALLGKINGAVGNYNAHI
                     VAYPNLDWEKFAQNFVEKLGLTFNPYTTQIEPHDAIAALFDAYARVNTVLIDLNRDIW
                     GYISLGYFKQKTKENEVGSSTMPHKVNPIDFENSEGNLGIANALLNHLSEKLPVSRWQ
                     RDLTDSTVLRNMGVALGHTLLAYDSCIKGLNKLEINHIAIQRDLQDTWEVLAEPVQMV
                     MRRFGIKNPYERLKALTRGKSGISQETLHQFIQALEIPDKEKQQLLALTPTNYIGLAE
                     KLAKDFHCRN"
     gene            35198..36358
                     /gene="hpnH"
                     /locus_tag="PK522_00155"
     CDS             35198..36358
                     /gene="hpnH"
                     /locus_tag="PK522_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HNC40173.1"
                     /translation="MGIPFLQKYRVGSYILKQKILGVKRYPLVLMLEPLFQCNLACAG
                     CGKIDYPDETLKRRLSVQECLDAVDECGVPVVSIAGGEPLIHKEMPQIVAGIIQRKKF
                     VYLCTNALLLDDRMDDYKPSPYFTFSIHLDGNKERHDTSVCRDGVYDKVIPVIKKALE
                     RGFRVTVNCTLFQNETAEEVADFFDTATELGVEGINVSPGFSYEHAPRQDVFLQRSVS
                     KRLFRAIFQEGKKRKRTWKFNHSSLYLDFLAGNQSYQCTAWGNPTRNLFGWQRPCYLL
                     VDEGYASSFRELMEDTNWDKYGVGKNPKCANCMAHCGFEPTAINDTFAHPIKAMRVSL
                     NGPKTDGPMAIDPSQQGSTTGASNAQKDFPIPVVVEPKAVKASSSGDVSNKK"
     gene            36437..37561
                     /locus_tag="PK522_00160"
     CDS             36437..37561
                     /locus_tag="PK522_00160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC40174.1"
                     /translation="MFEIFTVGFACFGALLWWIIVLLPWKPTSTKEYLESNPRLNSEQ
                     ISNVTVLIPARNESASIERTLQALSTQGKNLPIIIVDDQSEDDTSEKAKNFPVTIISG
                     TTLPTGWSGKLWALEQGLAHIKTSYVLLLDADIELDNGMVATLLQKAEHEQLAFVSLM
                     AEPNMDSFIERLLMPAFVFFFKLLYPFKLSNTPQSKIAAAAGGCILVKREALHQIGAF
                     NCLRNALIDDCTLANRVKQAGFLTWTGLSHSVKSHREYQTLMPIWDMVARTAYTQLNY
                     SIFWLLVCTLLMISLFWFAPVGSFLAEEWLGVMIGLFTWLAMLVAYIPTLQFYRRSLL
                     WVVALPFIGFLFLLMTWTSALKYWRGERALWKNRRYEISL"
     gene            37545..38180
                     /locus_tag="PK522_00165"
     CDS             37545..38180
                     /locus_tag="PK522_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpnM family protein"
                     /protein_id="HNC40175.1"
                     /translation="MKSRYNFILLRYLIIFSVVLFVPALSHSQPTESETPEQVINELQ
                     ATLIQVMQKGTELGYQERFNLLAPVVQRSHDLPAIIKAVLGTHWNKLTEEQHQAITET
                     FSKLSIATYAERFNQHDQERFEFIEKRTLPKEQVLVRSQLIQADGKAVNFDYVMRQGA
                     SHHWKIINILADGVSDLALKRAEYSAIIQRDGFSALLSLLEQKIIQAEQHH"
     gene            38299..40956
                     /locus_tag="PK522_00170"
     CDS             38299..40956
                     /locus_tag="PK522_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNC40176.1"
                     /translation="MKPTDQQERALIAWAAFCYRYAFWVLCFLLITGIACGTYTARNL
                     EVLTDTADMLSEELPFRVNLAKLNKAFPQYENTLILVLDAPTPEQAHAAASRLTSYLK
                     DGHIEAYDIYYGAGEPFLEQNGLLYKNIAELEFLADRLAAAQPLIVRIAQNPSLLTFI
                     HTLTEAVDAFRSGNNLELDPVLDGISATLSARLAHNPRPLSWQALFNDENPQSSYKEI
                     IILQPKLDYTELFAAKDSIDAIRAAFQTLNLENDFLTKLRITGEVALSYEELLSAMHG
                     AEYAGLVALIIVTLILFVALRSAGAIITVLVSLLLGLVITAAFTTATIGHLNLISIAF
                     AALYIGLAVDYAIHLILRYHELNRPDQPVIEILLRAIHDIGPSLLICALTTAIGFYAF
                     IPTTFLGIAELGIISGTGMFISLFITLTVVPALQRFLPSKIYSQVRKNRSITQQWLAL
                     PQKWPKTVYVLVALSFALALTTWNQIRFDYNLLNLSDPNGEAVLTFRELLADTQHSPW
                     LSMATVKSLEEANNLATQLTNLPVVDKVISIADFIPDQQDEKLQIIEGIALTLGPTFF
                     TENHVENDDLESSDFTQINQALENLRYQLNQLMIEKPNHLSIESWKRLSFSIDQLLTF
                     IHTVPAGQVQPFLNALQNDLIGQLPGVLNRLQLALEASTIKDESLPSSLRQRWHNESD
                     DFLVAVYPSENLNDNDALKRFIRSVQQHAPQISGTPAIMLAAGEAVVDAFIHAFILTL
                     LGVTLSLYVLLRKNILLVVITLLPLLLAALFTVATTALLNIPFNFANVIALPLLLGIG
                     IDSSIHMVYRSLSSVENQRSLLQTSTGSAIFYSALTTMAGFGSLILSTHQGTASMGML
                     LAVGILYILICVFIILPTLLTRFKHKLNF"
     gene            complement(40973..41920)
                     /gene="gshB"
                     /locus_tag="PK522_00175"
     CDS             complement(40973..41920)
                     /gene="gshB"
                     /locus_tag="PK522_00175"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HNC40177.1"
                     /translation="MNLVFIIDPLESLKIEKDSSIAMMQEAIVRDHRVFVMHQGDLSY
                     ENNIVMARVNELQPKQSGEIRAHWFDLGPKEVVPLAQFDIVLMRKDPPFDMEYIYSTY
                     LLEIAQQQGVLVINNPRSVRDHNEKLAITKFPDFIPATLVTRNEHLIREFLSQHQDIV
                     LKPLDGMGGASIFRVHSTDPNLSVILEMMTHYGTRTIMVQRFLPEIVLGDKRILLIAG
                     KPVPYALARKPKSGESRGNLAAGGIGIVQPLSERDLQIATTIGPVLNTLGLILVGLDV
                     IGDSLTEINVTSPTGMREITKQTGFNVAGMMIEALEALS"
     gene            complement(41949..43235)
                     /gene="gshA"
                     /locus_tag="PK522_00180"
     CDS             complement(41949..43235)
                     /gene="gshA"
                     /locus_tag="PK522_00180"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HNC40178.1"
                     /translation="MAVPHLTTALSGPILALEKRILEAMPAIEHWLRGKWQQHTIPFY
                     CSVDLRNSGFKLAPVDTNLFPGGFNNLNTDFLPLCVQAMMAAVEKICPEAHSILIIPE
                     SHTRNIYYLQNVHVLERIMRHAGLNIRIGSLIPEITEPTPINLPDGNTLLLEPIVRKG
                     NRLSVGNFDPCAILLNNDLSSGIPDNLKNLDQIIIPPIHAGWTTRLKSQHFTAYDRVA
                     QEFAELIGIDTWLINPYFNSCGKIDFYEEKGVDCVAATVDEILSNIKEKYTQYGVKEE
                     PFVIVKADAGTYGMGIMTVKDGADVYKLNRKQRNKMAKIKEGLSVSHVLVQEGVYTFE
                     SIHQAVAEPVIYMIDRYVVGGFYRVHTGKGIDENLNAPGMHFVPLAFDTSCVLPNHAA
                     LPDSVPNRFYAYGVVARLALLAAAVELEQHAPKVGA"
     gene            complement(43373..44476)
                     /gene="nirK"
                     /locus_tag="PK522_00185"
     CDS             complement(43373..44476)
                     /gene="nirK"
                     /locus_tag="PK522_00185"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HNC40179.1"
                     /translation="MNNKAVQAVIGLGLLCFTASVAFATKHDISKLPRVKQTLVAPPF
                     VPEHDQVSKGPKVVEVTMETIEKQIEIAPGVKYWALTFNGTVPGPLIVVHEGDYVELT
                     LKNPKTSTMAHNVDFHAATGALGGAQLTLVQPGEEVVLRWKALKNGVFVYHCAPGGTM
                     IPFHVISGMSGAIMVLPKDGLKDNKGKSVKYDRAYYIGEQDFYVPKGADGKYKSYDQP
                     AAGMADILGVAAGLIPSHVVFNGAVGAITGDNALKAKVGEKVLFIHSQANRDSRPHLI
                     GGHGDLVWQGGSFNDPPITNQETWFVPGGAAVAALYDFKMPGLYVYLSHNLIEAVLLG
                     AAAHMNVEGEWDNDIMEQLKKPASFNKDAAMDH"
     gene            complement(44770..44982)
                     /locus_tag="PK522_00190"
     CDS             complement(44770..44982)
                     /locus_tag="PK522_00190"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNC40180.1"
                     /translation="MAIRFTVLVLALITLITSCAFGGDASVKVQGTSTITTGQELQDL
                     QRALAENAITQEEYEAAKKALLHRSR"
     gene            complement(45003..45179)
                     /locus_tag="PK522_00195"
     CDS             complement(45003..45179)
                     /locus_tag="PK522_00195"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNC40181.1"
                     /translation="MCFLLVISGCSSTEVVQQPNLNVSIGQQFIDLKKAYDSGALTEK
                     EFQRQKARLIDSIR"
     gene            complement(45361..>45819)
                     /locus_tag="PK522_00200"
     CDS             complement(45361..>45819)
                     /locus_tag="PK522_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40182.1"
                     /translation="VSALPDIPLVREDHKVERSTVESELKGNQQSTMADLSVNTSAQM
                     IVEEPIDKNVIKMHLDAAEKAMRASRFTTPAKDNAFKHYQMVLAIDAHNDIAQAGLRR
                     IVDRYIQFIAKARLESRLEDAQLYLERAGSVLPNDERLEKIRLDLETTAH"
BASE COUNT        12885 a        10449 c         9246 g        13239 t
ORIGIN      
        1 gtaataggcc aacgagttgt cgattgttag gtgcatttaa cagcgcttgc ttgccaatga
       61 aatcccgctc acttttgaga tccactgtcc aggccagccc cgattcaagc ggatgggtcg
      121 tttcatccat atcttggcca tataaattca taccggcttc caggcgtaac gtgtcccgcg
      181 cccccagtcc ggccggcgca acacctgcag catgcaattt tttccagaag tcaaccgcat
      241 cctgggcagg caaaacgatt tcaaagccgt cctcaccggt ataacccgta cgggcaataa
      301 aatagttctc aaaagttgca gactgaaatt gttttaaatt ttccgtcacc gcctgagcac
      361 ccggaagcac ctgccaaact ttagcgcgtg cattcggtcc ttgaacagcg atcatcgcga
      421 gatcacggcg tggtttgatt tgaagctgtg gcgcaagact atcgcgttgt tgcataatcc
      481 aggcgatgtc tttatctgcc gtacccgcat tcacaactaa gcgaaaccag gattcggtca
      541 agaaatagat aatgagatca tcgatgattc caccttgcgg tgtcagcatg caagtataaa
      601 gggctttccc aggtagcgtt agcttatcaa cattgttcgc tactaggcgc tttagaaaat
      661 tgcggacatc actaccttca atatcgacga caagcatatg cgacacatcg aacatacctg
      721 catcacgtct aactttatgg tgctcatcga gttgcgaacc ataatggagc ggcatatccc
      781 agccaccaaa atctaccatt ttggcattca tatcgcgatg tgcctgatta agcggggtca
      841 ttttcaacac ggattttctc ccaaagataa taaatgaagt ctcattttaa ctgacctcac
      901 ccatctgtcc ttttacctga gagttttacg gtatagtaaa gctgatccgt gtgccccttc
      961 ggtggctgaa taatgaattc agcactctcc agattgcctg tcacaagcag tttttgaagt
     1021 taatcttgat actaaccata cctgagcgat tccgggggag ttacgccttc ggcggcacta
     1081 aaatgcgcgc tttcctgctt ggaaatttat attagcagtt tgctaatata acataccttg
     1141 gtttaggtaa taaagcagac tattaccgtt ataattctag ctagctcccc agttgggtta
     1201 cttgacaccc gagatgagca cttacaattt gctaagcatt ctaaaacttt gaccttagtt
     1261 tatctcgcac ttttccgaaa accagcattt gtctcttcta tgttgctatt cagccaattt
     1321 ttggatctaa aaaaacctga tgtgatgtcg tttagatcga tcaaatgata taaattataa
     1381 atgggggatc atgagtaatt ccgtattaga tttcatttgt agcgtaataa attgcgttat
     1441 ttcagctagc taattctagt taggtcatgg agtcgccaca atatattgaa cccactttcg
     1501 ccatgcgcgt gaagctggcc tcagcggggt tagcttcagc acttgcgagc gaagccttct
     1561 ctctttgggt acaaccaatt ttggtttggg tcgcaactct gcctacttgc gaatctttgc
     1621 cttgggtcag agcagagctt attgtcggca ttgcagtctg ctggttcatt agtgccattg
     1681 cgcttcgtca ggctgtggca acgtggcgtt cagggcaaac tccaactccc ggtacatggg
     1741 tctgggttcg cacgcgcata tacacaggag ttgtagtgca ggttacggct ttgggtctct
     1801 gcatggtcgc tgccgtgttc tctgttggcc ccatcgttgt cttcgcaaca caagagcttt
     1861 atgtcatctt ctgcattcct gtgccctgta gctgctagtt ctcggggtct gctgggaccg
     1921 gatcagcaag aattggactc aagtcttaca ttaccacaaa attagcttga gacattcata
     1981 agctcacaat cttaatcaat caactgaatg cttcaatcgt gatgagtaaa gtctgaaacg
     2041 atttaatcta atgactcact gattttctac cctgcaaaat cacggtaaat cttccccacc
     2101 aaaagatttg ggtaaccaag aaaaattaat aatcgtatcg aatctatttt attcttacgg
     2161 ccacgcttgc acttcatcta cggtctagca gctaacgtgg cacgccttag aaagtaattc
     2221 tccatcaaaa taactttgct aaaaattttt catatacgtg ttagcacgta tatgaattga
     2281 aatatttttt tattatattt tcaaaatgaa gcggtgcaga gaagtgaaga tttgttgtct
     2341 ttttaacttt tttgtatagg agatgaacag tggaggcatc taaaacatca acgaaaaaaa
     2401 gtaagtatca gacgtccgag aataatgggg aagttagtat taaagaagat cggcagttgg
     2461 attggcataa tcgcattgcg accgcagctt attacaaagc agaagcacgt agttttgagc
     2521 cgggacatga tattgaagat tggttagcag ccgaagcgga ggaaaaagat gaacaactta
     2581 cctgttgaaa cgagaagtac ggcagaagtg agagaaagca tttcgaagca aagcgataag
     2641 tatgcggcgc ttattctctc ggaagatggg atcattcagg attgcagcaa ggaaggcgca
     2701 aggctactcg gctatatgcc agaaaatttg attaagctac atatcacgaa gctattacct
     2761 aagctattag aaatcgattt actaaaggca catcaaatca acccttattt gcggtttctt
     2821 tcacgcatcg gccatcgatt cgagttaatc gaaatgaacg gagatcgtat gattggccag
     2881 ctatttttta gcgacttaga aaatgaaggg ggccatcagt tagtcgtttt gatcaaccct
     2941 atcccagaaa aaaagcaatt gcactaagcc agtttcaaat caacttagtg taaagcgagt
     3001 cttaagctaa gtggcaatac caaccctcaa aacaagaggg aataatcagt caaggagcat
     3061 atatgaagct agaaatcgta atgcccattg tgtcaagttt attgcttatt ctgatcggat
     3121 taatgttact gcctatcgaa tgaaggctat ccatagactg gttgaaatat agcttcaatc
     3181 ttagtaattt gttctctaaa aataagaaag gggctagatc ggataagcgg tatttcaacc
     3241 gagtgagcgc ctaatttcat ttaggttctt ccggttgttt atgcttttta tcacttcgat
     3301 attttactaa cacgtatcct agtgcaatac tgataagaat catccaccag gcgttctgtt
     3361 taagccattc ttctggatta ttctcgacgt acatttgaat ttctgcaagc tcaacgactt
     3421 tcttagcctg ttgcccacca ttcattgtct cgagatgcaa caataagcta agcgactgtc
     3481 gccctgtttt tttgggaata acagtccaag tccatagggt ctgacgatta ctggaaaaaa
     3541 gttctacatg cggtccaggg ttatcgatct gaaagttagc gccagccatt tcagcctgca
     3601 tacccggtaa tacctgaccc gttaggcctt gtaaccctgt tccatgtgac gatttaccga
     3661 taatatcatt ggtgcgttta atcaactcgc ttgtatccaa ttctagcgca acattaaacg
     3721 actgcaactc cttaacagcc tgtggcaaat taacagccga gtgatcaacc ggcaacttca
     3781 tcaggataac cggtggaaat tgctttatgc catacatgga atgtcctata cgattcaacg
     3841 tatagggcac caatagcaat ataatcagca atacaggtaa aattatttca aagctgattt
     3901 tgatcttggg catgctttct cctgagaagt tatttttctc agattttagc attcccccta
     3961 taatattgga caaagccctt taggttgatt actagctcaa gagaattaat cgatgaacag
     4021 caacccaacc attcccattc tagtcgctca tcgcggttac cccgcttgct atcccgaaaa
     4081 taccttagtt ggctatgagg ttgccttaca agcaggtgct tgctatgttg agctcgatat
     4141 tcagctcagc gctcgccata cccctttctt atgccatgat gacaatttga agcgattaac
     4201 tgggattgat caaaacatca ccgagttagc ggatgaaatc atcaaaacgc tatcgattgc
     4261 ttatccccaa tcaactattg ctgatgaggc aaccgtttca cccacgaaaa cagctccctt
     4321 tgccgattta ctttcatttt gcttgttact ggaaaaatgg ccccaagcaa aggctttcgt
     4381 agaaatcaaa tccgagagta tcgttaaatt tggattagaa ctaaccgtcg aaaccatttt
     4441 gcaggttcta cagccctacc gtgagcaatg tattatcatc tcattcgatt ggcaggctgt
     4501 tgcactcgct cgaaaaaaag gaatgcaaca aatcggctgg gtcatccccc ggtgggacga
     4561 tgcacatgcg aagatagcgg atacactcgc tcccgattat ttgttttgca gtatctggcg
     4621 cataccacca agcatcgaag aacgctggaa aggaccgtgg caatgggtcg tttataccat
     4681 caatgatagc caaacagccc tgcaatatgc acaacaaaat atcggaatga tcgaaaccga
     4741 tgccattggg gtcatgttga gcgatcccac gctaaaacag catgcttgct agccaatcct
     4801 atgatttagt cgttattggc ggtggcatac aaggaaccgc cattgctcaa gctgcagcag
     4861 caaatggcca tcatgtatta gttctagaga aaactggcct cgctgcaggc acatcgagtc
     4921 gttcaagcaa gctgattcat ggtggattgc gctatcttga acaaggtcat tttgggttgg
     4981 tacgcgaaag tttgcgtgag cgctcacagc tgctcaaact agcacctgat ctcgtcaaat
     5041 taagcccttt ctacattccg atttaccaat cgacttcacg aggcaaatgg acgattcgtg
     5101 caggattaac gttatatggt ttattagcag gattagggcc tgcatcccga tttcggagtg
     5161 ttcctcgcgc tgattgggca aagcttgatg gtttgtccac cgcaggtcta tcgcatgttt
     5221 ttcaatactg ggatgcacaa accgatgatc ggctgctcac acaagcattg atgcactcgg
     5281 ctatgaaatt aggtgcagaa ttatgctgcc ctgctgaatt tatgcaagcc aacctaacgc
     5341 aggatcgatg tgagctgaca tatcgctggc aaaaccaatc gcaccattgt acggctgctg
     5401 tcatcatcaa tgccgcaggt ccttgggtaa atcaaattct acacaacgtc accccctctc
     5461 cccccacctt acccattgaa ctagtgcaag gcactcatct ggtattagaa ggaaagcccg
     5521 gcagcggttg ctattacttg gaatcgcctg ttgatcagcg cgctattttt gtactgccgt
     5581 ggtatgagca cacgttatta ggcacgactg aaacaatttt tactggttta cccgaagcgt
     5641 gtcatccgtt agcagaagaa gaagcttatt taatcgaatg tttccgccat tatttcccca
     5701 atcgaaaagt tgtcgttcga gaaaaattct caggtttacg cgtgctaccg aaagcgcagg
     5761 gttcagcttt ctcacgctcc agagatattc aattactctg cgataatgtt agtcaaccac
     5821 gtattattac cctgtatggc gggaaactga cagtaagtcg ggcaactgcc cagaaagtgc
     5881 tcacaatggc agcttcttcg ctacctcaac gccatcccaa agcgaatctc gctgaacttc
     5941 caatcaaatt accgccttct gcatccgaat atgtctaacc aagcagttac cttagccatc
     6001 gaccaaggaa ctcacctatc ccgtgcccta ctattcgatc ccggtggaga agtgattgct
     6061 caattcagcg aacccgttga gatatttcat cgcagctcag ttgaagtgga gcaagatcct
     6121 gatgaaatca tcgattcgat tcacagtatc gtttcacaat cactgcaatt cgcagcacag
     6181 catcagaaac gtattagtca catcggtttg gcaacgcaac gttcgagtgt cgttgcctgg
     6241 cataaaacaa cggggaaagc gctcagtcca gtgctaagct ggcaagatcg acgcgcagcg
     6301 gattggttaa tgtcatttgc tgcacatcaa gccaccatca ttgaacggac tggcttgagg
     6361 ctttcccccc attatggggt tagtaaatta aactggctca ggaaaaacat accagcagtc
     6421 attgccgctg aagcacagca gcaattagcg tttggtccgt tagccagttt tattttattc
     6481 catttactcg aaggcaatcc cctcgtcgtg gatcacgcga atgccgcaag aaccctatta
     6541 tggaatatca cgagttgcga ttgggacgat gtgctgctga agttatttga aatccctcga
     6601 ccttatctac cccagtgcta tcccattcgc cattgttacg gttatctaaa aaatcattct
     6661 atccccatca cggcggttaa tggggatcaa acggctgcac tatgcgctgc tgcagaagtt
     6721 agccaggaag gggtttggat taacatgggt accggtgcct ttattctaaa atcgaccggc
     6781 tgccaactca ttcgccatcg caagctgctc agcggaattg ctgacagtga cgcacaggaa
     6841 aaacgatatg tgcttgaagg cactgtcaat aatgcgggta gtgcaatcga ttggttaagt
     6901 gaacatgaat cgatgcctca cccctatcag aagctggacg ctgcactacg ctcgatcacc
     6961 gatcccccct tattcatgaa taccattagc ggccttggct caccctggtg gggtaaagga
     7021 cccgctccac aattccacga tctcgatgga aagcccttaa caaatttacc agcggatcac
     7081 aggttagtcg ctgtcgtaga aagcattcta ttcttgatac aagccaatct cgactgtatg
     7141 gcagaaacac accttcccat caaaggcatt accatcagcg gtggattagc ccagtctgat
     7201 gctttatgcc aaaaacttgc tgatttatca ggcttaacgg tgcagcgccc cgcattggtc
     7261 gaagcaacgg cacaaggtat tgcatggtta gctgcgggaa aaccgtctgg ctggcataag
     7321 cagggtaaga tcgattgttt ttcgagtaaa gcgacaaccg ctcgtcagaa tgtcattaac
     7381 gaacgttatc agaagtttgt ttccatacta caatccaact aaaaatattc accgcgatct
     7441 ttggaggaac ctaaattggc tattactgga atgaatcatt ttacggtgct gacgactgat
     7501 ttagacaaga gtaaagcgtt ttatgtcagc ttgctgggat taaccgaagg ttatcgtccc
     7561 ccatttgaat ttccgggtgc ctggctttac tgtggggacc aggcgatcct acatatcatg
     7621 gcgggacggc ctattccggc tacaattcac ggtatcatcg accatattgc ctttagtgca
     7681 tgcaatcttc agtctgtgat agatcggtta aatcaacata acatcgcgta tacgctgaat
     7741 cgcctaaaag gacttgaaag ctggcaacta ttttgtgcag acccggatgg cgcaaaggtt
     7801 gaacttgatt ttgcagcaag cgaacccgaa ccccagaatg tcaaacaaac aaaataagtt
     7861 tatcccgctc gagttatcac tgaggtgatt tcgctgatag cgtgttagca aaatcacctg
     7921 ctttcacttg agtcagtcta gtcatatcaa agtgacggcg caaagcagtt aatatcaact
     7981 ccggtgtcaa agcagcaata tttttctcga atgcgatatc ccattgcagc gtacgattgc
     8041 gatgcaagta attgactagc cgttcagcga gcgctttatc ctgccctctt gctattctcc
     8101 gagcttcaag atagcccgcc ttagcaagtt caacctcttc tgcggtaaaa ccatctttta
     8161 gaacccttgt aaattcatca tctactgctt gttcaacacg atcacgattc tgaggtgcgt
     8221 agatggcgaa gattttgaaa tcgcctcgtg cttcaaaagc actcgccgtg agcgaagaaa
     8281 taacactgta tgaaagaccc tcttgttcac gaacacgacg ccacagccgt gaatcagccg
     8341 atcctccgaa taagtaattg cctagaacaa gcgcaggaaa atcaggatga tcgtctcgta
     8401 ttttgagatt cataccagcc tgataaaccg cattggcttt atctggagtt tccagattga
     8461 gatcgatagc cttgatatct tgatacggtg tcgaaatacg ttgatacggc ttagggcttt
     8521 tccagtcacc tagcgattgt tgcaaggtct cggtaatagc cttaggctca aaatcaccga
     8581 caatcgcaag ctctgcatcc gtgcttccat aaaaatcctg atgacagcgc ttgacgtcat
     8641 cgagggatac ttgattgatc cgctcaattc gttcttcgat tgtaggggta tagagccaat
     8701 gcgctgacgc gaaaggcatt agatgacgct cgagctggat acccgcttgc gcagcaggtt
     8761 cactcttttg attttcaata gaggacagaa tcgctagctt gagctgttca aactctttct
     8821 ctgggaaaat aggattgcga aacatttctg cgactaactg aagcgcatcg gttaaatgat
     8881 cgccagtcgt ttcaaaagca atgccatccg ctgtcatgtc gatgtgtact tttaagcgat
     8941 caaaagcatc gcgcaattgc tcacgagtat aacgctgtgt accccgtgat aacatcgcgc
     9001 ttgtcatgcc acaagcagtc gcacgattca ttttactttg ctcatctccc caatgcagat
     9061 taaacgcgac gacaacgcga ttgcctcgcg tttttttagg gagtaaggca agcttcattc
     9121 cgcctggtaa ttgcgtgcga atgagttttg cttcgatatt ctcgggcgtg gcttcaaacg
     9181 cttcccccaa actcagcatt gcgtttccac ggtattcttt gaacatgagt gcgagatcag
     9241 tgaccgctgg aatttctgca cgatctggtg tttttgttgg aatgaaaaga cctaatgttc
     9301 gattactggg tttaaaataa gtattcgcta cgcgctgtac atcttctaag gtaacttgtt
     9361 ggatccgatc acgatgtaga aacagcaatc gccaatctcc cattgcgata aattcagtga
     9421 tcgtcgtagc caaattacgt gtgttggtca gtaattgctc gattttatta agtaggcgtg
     9481 tacgtgcgcg ttccaattct tcagcggtaa tgggatgcgc tgctaatgtt tcgatggtac
     9541 taatcagcgt attgctcgca tgatcgagtg acatagtcgg tagcaaatta gcgccgaaat
     9601 aaacaaaacc ggtttcgcgt aattggcgat cgaaaccaaa aatcgctgtt gctaatttgg
     9661 attcgacgag cgatttatgc aaccgaccgc caggaacttg tttgatgatg gaaacaagca
     9721 aatcgattgc agcagattca ggatgtgcag cagccgggat atggtaaagc gttgctgcaa
     9781 tttgtgtatc gccaacccgg cgtagcataa cgttacgttc cccgtcctgc gtcggttcta
     9841 ccgtataggt ttttcgtagc tggcgttttg gatgaggaat agcgccaaag tgcttggata
     9901 tactatttag tgctgctgtt tcatcgaatt tgccggcaat gacgagtact gcattatcgg
     9961 gctggtaata atggcgataa aaatcttgta atcgctcgat gggaacattt tcgatatccg
    10021 agcgagaacc gatgattgcg cggccatagt tgtgccatac gaatgccgtt gatgccatac
    10081 gctcggataa cacaccaaaa ggattatttt cgcctgcctc aaactcatta cgaacaaccg
    10141 tcatttcgct atcaagatcc gctttttcaa tacgagagtt gatcatacga tcggcttcca
    10201 gcgagatagc ccaatctaag ttttctgtac tcgcagtcaa cacttcgtaa taattagtac
    10261 gatcatacga agtcgtgcca ttccaccgtg cgccccgctt ggtgaattca tttttgatat
    10321 cagggtggcg ttcggtacct ttgaatagca aatgttcaag caaatgcgcc atgccggctt
    10381 caccataacc ttcatcgcgt gaaccaacta gataaacgat gtttaccgtc accgtatcta
    10441 aggaatcatc aggaaggagc aagactttca agccattcgg catttgatac tcggtaatgc
    10501 cttcaactga tgtaatctga gttaagttag ccggtaatga ctgcatgggc tgctgcgcat
    10561 gccctaagct catgatactc aggctaataa tgaaagtagt gatgattaat aagtaacgcc
    10621 acatatcgta agttgaatca ttaaaatttt aaagactaaa tgttttgtag gaagttctgg
    10681 ttaatgatta aaatgcaaac ggttacgcaa tcactaatct ttttcatcac gcgcgtttga
    10741 ccattgcagt tttaaatcgc tcgctttttt aaatagactg agcagtgcct caccatttcg
    10801 attggcaagt tgttcacgta actctgttag cgcttgttga tacttatcga gctcagctaa
    10861 tagctgcacc cggttcgcta agcaaatgtc acgccacatc tcgggggaac tcgccgcaat
    10921 ccgtgtacaa tcacgcaaac tactgccagc ataactcaaa acatagttag gatcgaaatc
    10981 agtacaagtt tgaatatgat tcatcagtgt aaaagccagc atatgcggca aatgactcac
    11041 gatcgctagc atttgatcgt gttgctcagc agacatgatt gaaactttcg cgccacatga
    11101 ctcccataat actttgacca gattgattgc ttgttcgtta ttttccttca aaggcgtgat
    11161 gatgaggtgc ttctgtttaa ataataaggg atcagcagct tcagcgccgc gaaactccgt
    11221 tcctgcaatt gggtgagctg gaataaaatg ggcaagatga ttggtcaatt gttgctgcgc
    11281 agcttcgatc acattctgtt tggtactgcc cacatccgag actatcgttt gcttattcaa
    11341 gtaaggctcg atctgcgcca ttactcgctg aatttgacca actggggtag ataacataac
    11401 aaaatcagct tctgccagcg ccgcagccgg ggtaatcgcg atctcatcga taatacccaa
    11461 ctcacgcgct cgtagcatat tttcctgacc gcgtcccaaa ccaacaattt gcttaacaag
    11521 gcctgcatta cgtaatgcta atgcaaacga tccaccaatc aagcctacgc cgataatgac
    11581 aagtttttga agataggggg gggttggcat atcaacaaga atgaaggtat tccatgacta
    11641 ccatttaatg attaggctga attactgata acctgttcca atgcactcaa gaatttctca
    11701 ttctctgatt ttaagccgat cgtcacacgt aagtgatgag gcatttcgta aacgcctaac
    11761 ggacgaacaa tcacacctcg tttcaacaga ttttgataag ctttagctgt attaatttca
    11821 tctccccgga tacgtagcgt aatgaaattc ccataggaag gaatgaactc cacacctaat
    11881 tgccgcaatc cgttggtaat ttcagccata ccagcacgat tgagttcata agctcgtcga
    11941 acaaattctg tatcttttaa tgctgccaac gcgccaatca atccgagact attgacgtta
    12001 aagggttgac gaatacggtt cattaaatcc gctaactcgg ggtgcgtaaa cgcaaaaccg
    12061 acacgaatac cggctagtcc ataggcttta gaaaaagtac gggtaatcag taaattagga
    12121 aatttcttca accaaccgat gctattggct ttattggctt caggtaaata ctcatcataa
    12181 gcttcatcta agatcactaa tacattttga ggcactcgct cgacaaatcg ctgaagctcg
    12241 ttaggatcac acaatgtacc ggttgggtta ttagggtttg caataaaaat aatacgcgtt
    12301 tcgggcgtaa tcgcgttcaa catcgccact aggtcgtgac catagcgtcg cgctggcact
    12361 gaaatacctt gtgcccctag cgcttgagcg agcaacggat aaatagcaaa agcatgctgg
    12421 gaataaactg ctgccgtacc tggtttaaga aaaatccgtg cagcaagctc taacacatcg
    12481 tttgaaccat ttccaagcac aatttgatcg acactcactg cataatgttc agccagagca
    12541 ttcttaagct cataaccgct tccatcggga tatctcgcaa tttcattcag tgcaacagac
    12601 atcgcctcaa gtgccaaggg gctggttcca aggggatttt cattcgaggc taacttaata
    12661 acgctatctt tttccaaccc gagatctcga accaactcag aaatcggttt tccgggttga
    12721 tagggatgga ttgcgcggat atattgtgga gcaaattcaa agagtttcat gatcagtgtt
    12781 ctaggctgcg gggtatgatc ccagtatttt taagaaagtc gctttatcgc gtaattcacc
    12841 gattgcgctt gcaatattat tgttatcttg atgtccttca agatcaataa aaaacatata
    12901 ctcccacaaa cctttacgcg aagggcggga ttcgaatcgg ctcatactaa cttgatattt
    12961 agcaagtggc gcgagtaatt catacactgc accaggtcga tcatgggtag aaatggccag
    13021 cgaagttttg tctttgcccg atacgggaac aagctgcgaa cccatcacta aaaaccgtgt
    13081 agtgttatta ggatcatctt caatatttcg agcacagatc gttaattcaa aaatctcggc
    13141 agctttgcta cttgcgatcg cagccgcgtg gggatctgaa gcagcaagtc gtgctgcatc
    13201 ggcattacta gccacattga tctgcgctac tgatttaagt tgagcagatt gatgattaag
    13261 ccatccaata cattgcccaa aagactgagg atgtgaataa attttttgaa tttgcgtgag
    13321 gtctgaatgt tttgccatca agcaatggtg cacaggcaat tcaatttcac cacacacgac
    13381 caatggtgtc tgtaccagca aatccatggt ccttcccact gcaccctcag ttgaattctc
    13441 tacaggaacc acaccataat gcgctgagcc cgattcaact tgtcggaata catcatcgat
    13501 cgaatggcat gcaatcgtaa ccacggcatg tccaaaacgc ttagaaacag cctcttcgga
    13561 gaatgtgcct ggtggcccta ggtaagcaat agttaatggt gcttccatcg cacggcaagc
    13621 agaaataatt tcagtaaata aagcggtaat acgctcattg gaaagaggac ctgcgtttaa
    13681 ctgctgcagc cgagacaaca cttgcgcttc tctttcaggg cgatacgtta cagcattgcc
    13741 tttttgatgc cccactgctt gtgcaagttt ggcacgcgca ttcatgagct caagcaactc
    13801 attatcgatt gcatcgatct tggcacgtaa ttgatttaat tgttcagcca taatgataat
    13861 acgcagtata cgttttttat tgttgttatt ttagaccggt aagtatcgta ccttcagaac
    13921 gcctgtgata agagatattg ctttaataac ttgtttagat acaggactat cgacatcgac
    13981 caaagtataa gccatttcac ctctcgattt attagtcatg ttatgaatat tgaggcctgc
    14041 ggttgccatg caagttgaaa tctgaccaag cagattagga acgtttgcat tggctaccgc
    14101 aactcgatag ggagattccc gttccatgat tacatctgga aaattaacgg tattcttaat
    14161 attaccattt tgtaaatggt cgatggtctg cttcacaacc atgacagcac aattctcttc
    14221 tgcttctcgc gttgaagcgc ccaaatgcgg taaagtgata acccctttct cgttttgcaa
    14281 atgttgactc ggaaaatcac agacataata ttttatttta ccgctcttta ttcccgcaag
    14341 aaccgctgct tcactcacga tttcatcccg tgaaaaattg agcaaaatga cttcacgctt
    14401 gaaataatga atcagttgct cattgattaa atggcgggtt gaatcagcta aaggaacatg
    14461 aagcgaaata aaatcgctat gacgcaataa atcttcaatg ctctgcgctc tttgtacttc
    14521 tgcggaaaga ctccaagccg catcgacact aattttggga tcataaccca tgactttcat
    14581 tccgagcttt aaggctgcat ccgcaaccag tcgcccaatt tttcctaatc ccacgatacc
    14641 caaagtccgg cccggcaatt caatccctga gaaatgtttt ttattggcct cgacgagctc
    14701 attcaatttc tgattatcgc cttggagccc ttctacaaat tgaaaagcag aaacaagatt
    14761 gcgagaagcg atcaacatcg ctgccaacac aagctccttg actgcgttcg cattagcacc
    14821 gggtgtatta aaaaccggga ttccccgctg actcatcgca tcaactggaa tattgttcgt
    14881 tcctgcaccg gctcgcccaa tcgcgatcac atgcttagga atatccatat ccagcatatt
    14941 atgagaccgt accagaatga catcagggtc taccagatca tgaccgatta aatattgatc
    15001 agccggaaat tgcttgagtc ctactgaaga aatttgattc agtgtcagaa tcttaaaaac
    15061 ctgctgcgct tttttagcca tattgatttt caaattcttt cataaacgtg accaatgcct
    15121 taacaccttc ataaggcatg gcattgtaga ttgaggcacg catgccgccc accgagcgat
    15181 gacctttcaa ctgcacaagc cctcttgctt tggcttgctt tagaaattca atgtctaatg
    15241 cgctttgcct gagaataaat ggaacattca tgcgcgaacg gtcttgcagg gctactggac
    15301 attgatacaa atgactcgcg tcgattaaat cataaagtaa ttttgacttg gcaagattaa
    15361 gcgcctcgat cgcaggcacc cccccttgtt ttttcagcca ttccaacaca agacccatca
    15421 tataaatcgc ataagttggt ggtgtgttat acatcgagtc atgttgcaca tgcgcttgat
    15481 aactaaataa cgttggcgtc cctgcagtag gtgcttgcac cagatcttcg cggataatga
    15541 ccaataccaa acccgcaggc ccaatatttt tctgggcccc ggcatagatc aatccaaatc
    15601 gactgatatc cacaggtcga gataaaaaat tggaggacat atcggcgact aacggaatat
    15661 cggtacttcc ccccgcaatt tttgataaat cgggtatcca attgaattcc acgccaccaa
    15721 tggtttcatt cgatgtgtaa tgaagatacg cagcattgcg actcattttc caagtgtcaa
    15781 tctcaggtat tcccgtaaaa tgattaccca ctgatgatgc ggcaatattc actgtgccat
    15841 acttgctagc ttcttcaatc gctttttggg accattgtcc ggtattgaca taatccgctt
    15901 cttttttagt ttgtaataga ttcagcggaa ccatggcaaa ttgacttgat gccccacctt
    15961 ggagaaacaa aatcttgtaa tgattgggaa tcttgaccag atcacggaga tctgcttcaa
    16021 ctttagcaat gatcgacatg aattcctcgc cacgatgact catttccatc acggacatac
    16081 cgctttcatg ccaatctagc atttcgtcac gcgcctgctc gagcacttcc tgaggaagaa
    16141 ccgcgggccc tgcactaaaa ttgtaaacct tattcatggg attcattttc caagaatgag
    16201 taagcttgtc tcattattcc tcatccgcct caacgactcg ttccagtcca accaattttt
    16261 caccttcatc gagattgatc agcgttacac cttgagtcgc gcgccccatt tcacgaattt
    16321 cactgacacg agttcgaatc aatattcccc ctgtagtaat caacatgatt tcatcgtctt
    16381 gtttaaccag ttgtgctgca actaccttac cattgcgcgc tgaagtattg atcgcgatca
    16441 ttccctgagt tccacgattg tgtcttgtat actcgctaat cggtgttcgt ttgccatagc
    16501 cattttctgt tgctgttaaa actgttagtt cttcattctc ggcaacaagc aacgaaatga
    16561 ctttctgtcc tgcgcccaat ctcatccctc gtacaccacg agcacttcta cccaccggtc
    16621 taacatcgtt ttcactgaag cgcatcgctt ttccaccatc tgaaaataac atcacatcgt
    16681 gattaccttc agtgagcgca acaccaatca aataatctcc ttcatccaat ccaatcgcaa
    16741 taatgccatt gctacgtggc cgtgaaaatt ccgataaagg ggttttttta acggttccta
    16801 acgatgtcgc cataaagata taacgattat catcaaacgc tttaaccggc aagaccgcat
    16861 taattttctc tgctttttcc agctgtaata gattattgat cggtttacct tttgagactc
    16921 ttccgccttg aggtacgtta tatactttga cccaataaac gcgtcctaag cttgagaaac
    16981 acaaaatata gtcatgcgta ttcgccatga acatattgtc gataaaatca tcttctttgg
    17041 tggcactggc cagttttcca cgccctcccc ttttctgtgc acgatagtca tctaaaagtt
    17101 gtgatttaat gtacccagca tgggatagcg ttatcacgac atctgctggt gtgatcaagt
    17161 cttctgtgtt gagttcctgt gtattcgtga caatttcact gcgccgttga tccccaaatt
    17221 gatctttaat ggaacgtaat tcatcaacga tgatggctgt aattcgatcc gcattcgcga
    17281 gaatatcgag aagatcgatg atttgatcaa taacctcctt gtattcattg acgatttttt
    17341 gttgttcgag gccagtcaag cgttgcaatc gaagatcgag aattgcttgc gcttgcacat
    17401 cggataattg atagccatga tcaaacaaac caaattcaac accaagtcct ataggacgaa
    17461 aagcagctgt atcctgtgct gaacgggcca gcatttccgc aaccaattga gagctccagg
    17521 cttttcctat caatgctttc tttgcttctg ctggtgttgc tgccgcttta atgagtgcga
    17581 tgatctcatc tacatttgaa agtgcgacag ccaatccttc taataggtgc ccgcgctccc
    17641 ttgctttttt cagctcataa acggtacgac gggtaacgac ttcacggcga tggcgtaaaa
    17701 acgcctggag caattgcttt aaattgagta atcgcggctg tccatcgatt aatgcaacca
    17761 tattgatacc gaaagtatct tgcatttgcg tttctttata caagttattc agaatcactt
    17821 ccggtatttc gccacgcttc agctcaatca ctacccgcat cccggattta tccgattcat
    17881 cacgcaaatc cgatatccct tcaatctttt tatcgcgaac cagttcacca atccgaatca
    17941 gcaaattcgc tttattaacc tgataaggca gctcatcgat aataatgctt tgacgtgacc
    18001 ctttctcaag atcttcaaaa tgagttcgcg cccgcattac gacgcgccct ctgccggtaa
    18061 gataaccatc tctaatgcct gatgttccat aaatgattcc agccgttggg aaatcagggg
    18121 cttggataca ttcgataatt tcgtcgatac tggtttcagg gttattaagc aaacggagac
    18181 aagcatcgat tacctcgctt aaattatggg gagggatatt cgtcgccatg cccacagcaa
    18241 tccctgaaga accattgatt agcaaattag gaattttggc tggaaaaatc agcggctctc
    18301 gctcagaacc atcataatta ggaccaaaat caaccgtttc tttgtccaaa tcggccagta
    18361 attcatgcgc aatttttgcc atacgaattt cggtataccg catggctgct gcattatcac
    18421 catcaaccga accgaagtta ccttgtccat cgatgagtac ataacgcaat gaaaaattct
    18481 gcgccatcct gacaatcgta tcgtagacag ccgtatcccc atgcggatga tatttaccaa
    18541 tcacgtcacc gacgatacgc gctgattttt tatagggacg attccaatca ttcgaaagct
    18601 cgtgcattgc aaataacact ctgcgatgaa ctggcttcaa accatcccta acatccggca
    18661 gcgcccttcc aacgataacg ctcatcgcgt aatccaaata agaacgacgc atttcctctt
    18721 ctaggctaat cgtaagtgtc tcttttgcaa attgatttat tgacattggg tctgtttgat
    18781 tttgaaagta ttctcaatct attttgttaa agggcagatt gaaataaaaa ttaacttatc
    18841 ggttaaaatt aataaaagtg gccatccaac aaagaagtaa aatgctactt gcttaaaggt
    18901 tgctatattt tagcacgcca ttcccgtcca tttttttata aagtttgaag aagcatggag
    18961 ggaaggaaat aatttaggtg acgtggcgtt ctacgccaaa tcagtgcaaa gcgtggaaat
    19021 agccaaaaag cgcttgataa agcctactaa ataagtaaat aataaaaaat tacttaatat
    19081 tgcttgattt tcaatttgat aatacagcat agtttcaaat ttctcctttt taacttttta
    19141 gagatgagta atccactgtg tcttatttga tgaacacgta tcaaagactt ccggtaacct
    19201 ttgttaaggg ttcaggggtt tggctatggg ataacgatgg taatcgctat ttggatgcac
    19261 tatcgggtat cgccgtctgc gggttaggtc attgccaccc cgctttagcg aaagcactca
    19321 gtcatcaggc tgaaacactc attcatactt caaacctata tcacattgaa aaccaagaat
    19381 tattggccga gcggctggct ctgttatcag gaatggataa agtctttttc tgcaattcag
    19441 gggctgaggc caatgaagcg gcaataaaac tcgctcgcct ttatggtcat caccgcggga
    19501 ttgagttacc gacgatcatc gtgatggaaa aatcgtttca tggtcgtact atggccacac
    19561 tcactgcgac gggcaaccgc aaagcgcaag ctggctttga acctttactc actggattcg
    19621 tacgagttcc ttacgatgat ctcgttgcag ttgaacaaat tgcagccaat aacaagaaca
    19681 ttgtcgcgat cctagtagaa acctatcaag gcgaaggggg tgtcaacttc cctcaagtta
    19741 actacctaga tggtctgcgt cggttgtgca atcaaaataa ctggctattg atgcttgatg
    19801 aagtgcaatg tggcattgga cgaactggaa agtggtttgc ctttcagcac agcaatagta
    19861 gtccagatgt gatcacgctt gccaaaggac tgggttcagg tgtaccgatt ggcgcatgta
    19921 ttgcaaaagg accagcagcg gagattttta aaccaggcaa tcatgcatca acctttgggg
    19981 gtaatccttt agtatgtaaa gccgcgctca cgacacttga agttatcgaa caagatcact
    20041 tgatgcaaaa tgctgtctca atgggagact tgatgcggaa tcgttttcat gaaaagctag
    20101 caaactggtc taacgtcatc aagattcgtg ggcaaggcct aatgttaggt attgaactgc
    20161 ctattgcatg cggagaatta gttaaggaag cgctagctgc aaaattattg attaacgtta
    20221 cttcagaaag agttgttcgg ctactacccg ctttggttat tactcaagtc gaagctgaac
    20281 agattgtgga taagacatca tcgcttattg atcgatttct aacgacgcag tataacaata
    20341 cgacgcctac gtaaataaag taaatcgata acaacgacct catgactccc aagcatttct
    20401 tgaagattaa tgattttacc ctcgatgaat ttgaatatct attcgagcgc gcgcattgga
    20461 tcaagaatga atttaaacaa tatcgtaagt attggccttt aaccgacaga acactggcca
    20521 tgattttcga caaacactct acccgcacac gattatcatt tgaagccggc atgtgtcagt
    20581 taggtggcag cgtgatttat ctttcaacgc gtgataccca gttaggacga ggagaacccg
    20641 ttgaagacgc agcgcgcgtc atttcgcgta tggtcgatat catcatgatt cgtacctttg
    20701 agcatagcat catcgaacga tttgcaaatc attcccgtgt tcccgtcatt aatggcctta
    20761 ctgacgagta tcatccctgt cagattattg gggatatttt tacttttatc gaacatcgcg
    20821 gaagcatcaa aggtaaaacg attgcttgga tcggtgattc gaataatgtt tgtaatacct
    20881 ggctccaagc ggctgaagtt ttcgacttta atgttcatgt ttcaacaccg cccggctatg
    20941 aagtcgaacc tgaacgagca gggttatacg gcaccaatca ctacgaagag tttagttctc
    21001 cccatgatgc ggtgaaaaat gctgatctcg ttacgaccga tgtttggacc agtatgggat
    21061 ttgaagatga gactgaaatc agaaaaaatg cattcgcgga tttctgcgtt gatgatgaaa
    21121 tgatggctta tgccaaagca gatgcgctgt ttatgcattg cttacccgcc catcggggtg
    21181 aagaagtaac caatgaagtc ttagagggga ggcaatcggt tgtttgggat gaggcagaaa
    21241 atcgattaca tgtgcaaaaa gcattaatgg aatatctgct gcttggaaaa atttagcaac
    21301 ccgtgcgtta tcacggaatc aattgaacta cgaaaataaa aaaagcgatg aaaaaagtta
    21361 aaaaagtggt cttggctttc tcaggtggac ttgatacgtc ggttattttg aaatggctac
    21421 aagaagtcta tcaatgcgag gtggtaacgt ttacagcaga tattggccaa ggtgaagaaa
    21481 ttgaacccgc gcgtgcaaaa gctcaacgac ttggcattaa agaaatcttt atcgaagatt
    21541 tacgcgaaga atttgttcgg gaatatgtat tcccgatgtt tcgtgcgaat accatctatg
    21601 aaggggaata tctgctgggc acgagtattg cacgcccctt gatcgcaaaa agacaaattg
    21661 agattgctcg ggaaacaggc gctgatgcgg tatcgcatgg tgcaactgga aaagggaatg
    21721 atcaagttcg ctttgagtta ggttattacg ccctgcaacc ggatattcaa gtcattgcac
    21781 cgtggcgaga atgggatctg acttcgcgtg agaaattatt aagttacgct gaacgccatg
    21841 gaatctctgt cgagatgaag caaaaagggg gttctcctta cagtatggat gctaatttac
    21901 tccatatttc ttatgaaggg cgatctctgg aaaacccggc attagaacct gaggagtcaa
    21961 tgtggcgttg gacacaatcg ccggaaaaag cacccaatga atctgaatat atagaactag
    22021 aatatgaacg aggagatatc gttgcaatta atggtaagcg cctaacccca gcaaccattc
    22081 tcgaacaact caatcaactg ggtggaaaac atgggatcgg tcgccttgat cttgtagaaa
    22141 accgttatgt cggtatgaaa tcacgaggct gttacgaaac ccctgggggt accattatgc
    22201 ttcgtgccca tcgtgccatt gagtctatta ctttggatcg agaagtcgct catctgaaag
    22261 atgagctcat gccccgctat gcatcattga tttacaatgg ttactggtgg agtcctgaaa
    22321 gaatggcatt acaaacaatg atcgatgaat ctcaagtcca cgtaaatgga tgggtacggg
    22381 taaaactcta taaaggaaac gttattattg taggccgaga ttctaaaaca gattcattgt
    22441 ttgataccaa tattgcaacc tttgaagatg atgcaggcgc ctataatcaa gcggacgctg
    22501 ctggatttat tcgcctcaat gcactccggt taagaatcgc tagtaaaatt agagccccca
    22561 gaaaataggg agtaggggaa ccgaattccg cttctttgat tacattaaac ttagcgattc
    22621 tgcgcaatga tttgatcggt tatatagggt aaaatgatct ccatcagttg cggaaaaatt
    22681 ttaggattac ccgccacgac ctggccactt tttagataat gatcattccc ttccagatca
    22741 ctcaccatcc cacctgcttc gataatgagt aaacacccag cagcgatatc ccagggcgac
    22801 aaacccattt cccagaaacc atcgtatcgc ccagcagcca cataagcaag atcaagcgct
    22861 gcagatcctg gccgccgaat gcccgcggtc ttgggaataa ccgctttaaa catagcaaga
    22921 tacgcttcca taaaggaaag gtctcggaat ggaaaaccag ttcctatcaa actttctgat
    22981 aaattcgttc gcttactgac acggatgcgg tggtcattga ggtaggcgcc gcggccacga
    23041 ctcgcagtaa aaagctcgtt gctaaccgga tcataaataa ccgcttgtga taagacaccg
    23101 cgatgtaata acgcgattga cacgcaatat tttggaaaac catgcaaaaa attggttgtt
    23161 ccatccaacg gatcgataat ccattgataa tcagactgct tttgacctga ctgactgcca
    23221 ctttcttctg ctagaatggc gtggttagga tacgcatcca ataatatttt tatgattgcc
    23281 tcttctgcag caccatctac ttcactgaca aaatcactgt gtgatttttg ggtaacgttc
    23341 agaatatcca gatttctaga agcctgatta ataatgttgc ctgcacggcg cgcagctttc
    23401 actgcaatgg taagcattgg atgcatgtta attgatcaat aaattcaaca attcagtatt
    23461 ctaatatgat tcaccactct ccgcttgata atgtccgagt tgttctcagt cacacgagtc
    23521 atcccggtaa tattggagct tcggctcgcg ctatgaaaac catgggttta agctcacttt
    23581 atttagtcaa tcctaaatca tttcctgaca atgaagctta tgttcgcgca accaatgctc
    23641 gagatattct cgatcaagca gtgatttgct caacgcttga tattgccctg caagactgtg
    23701 tattagcagt tgcaatgaca gcacgcgctc gtgaattaac ccatcaggta atcgatacga
    23761 ggcaaatcgc tcaaaaactt gttgaaatcg caacggaatc acccgtagca ctggtttttg
    23821 gaagagaaag ctctgggcta acgactgctg aagtcaccaa atgccagctc accgtccata
    23881 ttccgaccag ctcaacctat gcatcactta atcttgcagc cgctgtgcaa atcatgtgct
    23941 acgagttacg tatgggcgca gttacaccag aaagcacatc acctccacct gaagtgccac
    24001 aacttgccag cttgaatgaa attgaactct tttatcagca ccttgaggaa gccctgatta
    24061 caagcaaatt tctcgatcct cagcaaccca aacgcctgat gcaacgaatt cgtcgcctat
    24121 tttctcgaac cgccttggaa aaggaggaag tcaatatatt gcgtggaatt attcatgcgt
    24181 tagcgaagga gaagcgataa ctatatcgaa tttacatgac tggattgact tagtagtgtt
    24241 atttagcgat gattgcagat gatatttctt tattgaccct acttcttata tttaaggaat
    24301 gattatgaca acgaatacga tctttaaaca taaagttagc gcgattatta acaataagca
    24361 cttacttaaa catccgttct atatggcttg gaccgaaggg aaattaaccc aagaacagct
    24421 tcgccattat gcagaacaat atttttacaa tgtattagct gagcctacct atctcagcgc
    24481 cgttcacttc aatacccctc atggtcatac tgaggaaaac tgcggtgata tcagcgtacg
    24541 tcaagaagta ctcaaaaact tgattgatga agaacatggt tcaggtaatc atcctgctct
    24601 gtggaaagcg tttgcatttg ctttaggtgc gaatgataaa agtctagcaa atgccgcagc
    24661 actgccagaa accgataact tggtgtcaac tttccgtgat atttgcttga atagcccgtt
    24721 ctatgttgga ttggctgctt tacacgcatt tgaatcacaa gttcctgata ttgctgcagt
    24781 caaaattgaa ggtttggcta aattctatgg aatgaataat ccgaaagatt atgaattctt
    24841 ctcagttcat caaaaagccg acatttatca ttctgaggca gaatggtcaa tcatcgagcg
    24901 gtttgcagat acgcctgaaa aacaagcagc agttttagcc gctacacagc gcgcttgtga
    24961 cgctttatgg aaattcttag atggtatcca tgaaaattat tgcgctaact tgatttgtga
    25021 tcagcaaaca gcggttacgc ttcattaatt aacgcggtgg atagatgagt tttatctcat
    25081 ccatccaccc gcttattgca atattcaatt agaaaactgc actgaagcca acttgactac
    25141 taaaccccgc ttctggcgca aagctgcgta aaaacgaagt ggaattacga atcgtcgcat
    25201 tggtcatgtt agacagcttc gcaaagaaga gaatcttcat acgatctgat gctttccaat
    25261 cataagtgat atcaagatct aatcggttat agcccggtgt ctcagtctct agaagacctg
    25321 gtctattttg ctccaacgca tgcgtatagc gcaaaaggct tctccattgc atccattgca
    25381 atccaaactc agcaccaaac cgcaatggtg ggagtcttgg aacatctccc aatgatccac
    25441 tttggaatga acctcgtaca taatctgaaa agaatgtgaa gcgcggatta taagtagcag
    25501 ctattttagg gcgcatggta atttcagctt catagcccca gaaatttgca gtatcagcct
    25561 gatagccaaa aatgggtagg caatttctta aatttgcaca agctaattgg aaacgcgggg
    25621 gatcgggcgc aaagttaaaa aagaaacctt gattttttaa ataaataaaa tcttcaacag
    25681 atttatagta accattcgca ttaaaagaaa tgacattacc agcgtaacgt agccctaatt
    25741 cgtaatgatc tgtttgttca gtatttaaat ccatgcgccc taattgatag ctacgcgtag
    25801 caaaattagg acccgatgca aatagctctt gaacatcagg gctacgttgc gcacgttgcc
    25861 agtttaggaa aaatgacgtc agtggcaata tttcgtaatt taagctagcc gccagcgaat
    25921 aggtcaaata attgagcgaa ttaggcagcg gcaacggcga ggggataccg gcaagccgca
    25981 gctcattgct tttaggcgag gtggtttggt gctctattcg cccccctaat tcaaatgtcg
    26041 ttcgatcatt aacgactagt gtctcggtta gaaaaaaacc gattgaatct acattcgatg
    26101 atggggtaaa cgtttctaga cctgtcgcag aaaaattacg gttctgccat tgcatcccaa
    26161 atttaccatc caaccaatct cccttctggt gattcatctc cagacgagat tcccaaacag
    26221 aattaccaaa tcgggttagt gtagagtttc ttgctgatat atttttatca aagtctttat
    26281 gatcgtaatc gatatagctg agcttggctt tgatcgatgg caatgctccc cagtaggttt
    26341 tccagaaacc ttctaaatcg tagcgtcgct gattcatatc aatacgaatt gcttcttgtt
    26401 cggatcgact actgtcaccg tgaccaggaa cacctggagg gataccatag tttcttgcag
    26461 tttcatgata gctcccaccc aaataaccat attttcctac caaagaaatt ccagcagagc
    26521 cgccctggct atgtgaatgg ctattttgaa tcacattatt actattcgta gtaggttcta
    26581 cctgaaattg ctgccgaatc gccgattcat ccaatgctga gccgggaatg tgcatgttat
    26641 tggctgatcg atggaagtaa tcaaaatgca aggcaaccat gctgcgccca atatccaaac
    26701 ttatcattcc cgccttttca cccggattat ggtcaaaacg aaaatcagtt ctaccggaca
    26761 tgccattcgt tggaattcga tcaggaatac gctggtgact gatatcgacg acacccccga
    26821 ttgcgtttgc tccatactgc agggttgctg ggccttttat aaccgttatt ccattcgcca
    26881 gaatgggatc aaaaggaatt gcgagatcgg ggctaatcgc agaaagatca tgcataccga
    26941 tcccattctg catgactcgc acgcgaggac cgctcatacc tcgaatgaca ggctgcccta
    27001 caccgggacc ataggaaaga ttgctaacgc caagctctcg ttcgagcatt ctgcccagcg
    27061 tactttctgt acgcaaacgc aattcattac cttttaactt ctgaataggc tcagcagtta
    27121 actctgatgc agctgatgtt tcaaccgtaa tcgtttcaaa tttaactgta ttgctgggat
    27181 tgggtgtaat tgcaggaatt gcagatgaat cagcaacaac aacaggtttc ggttggttaa
    27241 attgattggt tgctaggaca ttacttatcg ataaaactaa agccaaagcg aaaaccatgc
    27301 cgatcaatct gatcagcaca ttgttgcacc gctgtatcat aaacctgctg cttgatagaa
    27361 agtctcaatt gcaccatgat cagtaaggcc gcccaactca ccaccgagcg tataaatttt
    27421 tccatctaaa ctgccaaggc aagtaccatt gttgtaacac cgtgcataat agccttcgat
    27481 aaaacgactt tttactgcgt gtgggaagta acgactgtaa ttctcctcag cccaattaaa
    27541 aagtgcatct atccgactat aattgtttaa cttgagcact ggagagacat ccaaagcttc
    27601 gctcaatgct aaaccattaa aattcccttt tgcgttgata tgaaaaacta atgcgaacgg
    27661 ctgggagggt tgataaagaa tattttctcc cgtcgcatca acaccgagaa ctgtaatgat
    27721 caccatatag gcaccatcct ccggtacttg accatctggt ttctgaattt tgaaaatggg
    27781 atgagattcg aatgtgctgg tattggttaa attttcaata aatccttgaa tccctttacc
    27841 ttctgaagcc gatattcgct tttcatctga taatcgacta atgataattt gttctgagaa
    27901 tccttcggtc aaccattgcg attgctcact tgaccatttc atcaaatgcg tttctgcttg
    27961 atacccaaga atcccccctt gatcaaattc tatgccggtg aatcctgcat cacccatacg
    28021 ataaggagcg atggaatcag taaatcgaat tacaaaagcg ctaagatcac ccaatttagt
    28081 gccttgtttt ctgatatcaa tttcaataac cccctctcgc caggcaaagc ccatattggg
    28141 ttcaccttca tgagcaaccg cattgatacc aaagctgatt agtaagatta ccaaaacagc
    28201 acaattaaat tttttgatca ttattcttaa ttaaactcac tcgatttatt gcgggtgata
    28261 ctagctcagg ggaagttgaa aggcaatgcg acaaattgtc gcattgcccc gatagggaat
    28321 atttgttaga ttggtacgcg tctataccct gtcaaccatc atttcagtta tttcttcatg
    28381 tcaccttatc gcttgatttt cgctgctgta ataagcttac tcatatcgct cccctcattt
    28441 gctggagagc tgcaccaaca tattgaagtc aatacaacga atcaacgtgt gcaagtcaat
    28501 ttttgtcgcc ctgaaagagg ctgtagtttg gtaaaaccag aaacgattgg attaccgcca
    28561 ggccagctcc cgcttgataa gctaacgggt acagcaattt acgtttctga atttacggcg
    28621 cttcctaacg gaattgcaat tgatagccct ggatttgaag gtattgcggg tgatttgagt
    28681 gcaggtgacc gtgtccatta cgtggcgagt agccgactta gtttctggaa tcctgctgaa
    28741 cgtcagtggt ctttagcccc agaaggaatc cgtattcgtt tagcgggtgg tttagattta
    28801 cagcccgatc aatcctgtgg tcaagtgttt tgtcttccaa aagctgtcga gggatttacg
    28861 atatttaatc gacaaggtgt ttcaggcatc aactcgttaa tcgtgggtga agctcgtcct
    28921 gatggctcat tacacaccca tctggattgg attcttgaaa cagaacaagg tgctccgaat
    28981 ggaccgatag gcgcttattt agttgagtta agtataagga ctgatgctta tcccactcct
    29041 tctgcacctt tctggatcat gtttaatcat agtctaccaa tcactgaatt tcagcaagcg
    29101 gttgcagacc gagttttgaa aaacagcatt gatactgctt tggctgataa gctttttgac
    29161 tgggctgaat caaattacct atcgctattt ccgaataaaa ctacttcttt tattgcagta
    29221 gggtattatg cgcgctgcta tcaaaacgga acttgtgtcg gggttagaga taattatgtt
    29281 tttgctgttg gtggagaatt cggtcccaat gtagtcaatc ttggagaatt ttcttcatta
    29341 ttaaagttgg caggatttta gtttaggtct gaagaaaaag tttttcgatt tttcatactt
    29401 ataaaaatta atgaatctgg cgaggctgga tgtgtaatta aaacatctct tggagatgtg
    29461 gcatgaacaa tatcgtgaac tcagcctcgc cagattcgtc aacccctatg caggggaaga
    29521 ttactctgag aaaaattcac gcgccttttc catccatgac ttcgctcgag gactatgcct
    29581 agaattatct ttctgactaa tatcctctaa ctcttgcaac aattcttttt ggcgcgcggt
    29641 caaattgacc ggcgtttcaa ctgcaacatg acataacaaa tcgccagcag aattactgcg
    29701 tacccctttt atacctttac ctcgcaaacg aaatatctta ccggtttgag tttcagcgga
    29761 tatcttgatt tttgcatgtc cctccaaagt agggacttca atctctccac ctaaggcagc
    29821 cgttgcaaaa ctgatgggta tttcacagtg caagtcgttt ccttctcgct gaaatacggg
    29881 atgacttgat agttgaatta caacatacaa atcgccagcc gggcctccgt tgattccggc
    29941 ttcaccctct ccagataatc gaattcgatc tccatcatca acccctatcg gtattttgac
    30001 atttagcgtt ttatgctgct taatacgccc tgcgccatgg caagaagagc aaggcgaagt
    30061 gatcattttg ccgctaccat gacatttcgg gcaagtttgt tgaatcgaaa agaaaccttg
    30121 ctgcattctg acctggccat gcccatcaca ggttgagcag gtttttggcg atgttccagg
    30181 cttcgcacca gaaccattac aggtatcgca agtcgtcatc gtcggaatac gaatttttgt
    30241 ttcggaaccc cgcgctgcct gctcaagcgt aatttctaga ttataacgaa ggtctgcacc
    30301 acgatggaca ttggagcgcc ctcctcctcc gcgcattcca aaaatatcac caaaaatatc
    30361 gctgaacgcg tcaccaaaac cctgtccatg gccagcacca gcagcactgg tatccacacc
    30421 tgcatgtcca tattggtcat aagctgcacg cttactggca tcagacagta tttcataggc
    30481 ctctttggct tctttaaatt tttcttcagc cttcgtatcg cctgcatttc gatcaggatg
    30541 gtgtttcatt gccaatttac gataggcttt cttgatttca ttctcatcgg catccctgct
    30601 aatgcctaga acttcataat agtctcgctt agccatataa gtttcctgtt acatgggtac
    30661 aatcaatatc aattagtact gccattgatt atttcccaat gggtataacg taattatctt
    30721 gagcattaat gaaataatca ttgctcatct caaaacaaaa cgcagagtat gcaagaaaaa
    30781 gaattgataa tttcttgcat actctgcgtt agtcccaaaa taatttacaa tttatttctt
    30841 cgttttaact tcctcaaatt ctgcatcgac tacctcacct tctaccgttt tttcgccccc
    30901 ttgctgagcg gttgattcgg tttttggctg ctcactttgt gtttgatcct gagcgtagat
    30961 tttctcggca agcttttgtg acgcttgagt caaagcttgg gtcttagcat caatgatatc
    31021 tttatcattt gatttcagcg cttcctcagc ttctttcagt gctgcctcga tcttagcctt
    31081 ttcatcagct tcaagcttat caccatattc ccctagtgat tttctaactg aatgaatcat
    31141 tgcatcacat tggttcctgc tattaaccaa ttctagtgcc tttttatctt cctctgcatg
    31201 cgtttcagca tctttgacca tacgctgaat ttcatcttcc gataaacctg aacttgcttg
    31261 aatcttaatt ttgttttctt tacccgttgc tttatccttg gccgatacat gcaaaatacc
    31321 atttgcatca atatcaaacg ttacttcgat ttgaggcatg ccacgcggag cgggaggaat
    31381 atcggtcaag ttaaattgac cgagactttt atttccggaa gccatttcac gttcaccttg
    31441 cagcacatga attgttacag cagtttgact atcatcggcg gttgaaaata cttgatttgc
    31501 ttttgttgga atagtggtgt ttttttgaat caactttgtc atgacaccgc ccaatgtttc
    31561 gatacccaac gataatggtg tcacatccaa caacagcaca tcttttacat cgccttgcaa
    31621 caccccacct tgaatcgcag cgccgatcgc aactgcttcg tcaggattta catctttacg
    31681 aggctcttta ccaaaaatct ccttaacttt ctcctgtact ttaggcattc ttgtttgtcc
    31741 gcccaccaag atgacatcat ggatatccga gaaagataaa cccgcatctt tgattgcaat
    31801 tcgacatggc tcaatggtgc gctcaatcag ctcttcaacc aaactttcta atttagcgcg
    31861 tgtgatttta atcgcaagat gcttaggacc actggcatct gccgtaatgt agggcaaatt
    31921 cacctctgtt tgctggcttg acgatagttc gattttagct ttttcagctg catctttgag
    31981 tcgttgtaac gctagcatat ctttcttgag atcgatgcca ctttctttct taaactcgtc
    32041 aactagatat tcaataactc gagagtcaaa atcttcacca ccgagaaatg tatcaccgtt
    32101 ggtggcaagc acctcgaatt gatgctcacc ttcaatctca gcaatttcaa tgatagaaat
    32161 atcgaatgtt ccgccaccga gatcataaac cgcaattttc cggtcacctt cttttttatc
    32221 catcccaaat gccaatgcag cagcagtggg ttcattgata atgcgcttta cttccaagcc
    32281 agcaatacgc cccgcatctt tcgttgcctg ccgttgtgaa tcattaaaat aagcaggaac
    32341 tgtgatcaca gcctcagaca caggttcacc caagtagtct tcagcagttt tcttcatttt
    32401 aatcaaaact tgtgctgaga tttctggggg tgctattttc cttccacgta cttccaccca
    32461 cgcatcatta ttatctgcac gaacgatact atagggcacc atcttgatat cgcgttgtac
    32521 catgtcttca tcaaagcgac gaccaatcaa tcgcttaact gcgaacaagg tgtttttagg
    32581 attggtaaca gcttgacgtt tcgccgaagc cccgacaaga atctcgttat catcggtata
    32641 agcgacaatc gatggtgtag tccgcgtacc ttccgagttt tcgattacct taggtttacc
    32701 attctccatt actgccacac aggaatttgt tgttcctaaa tcgattccta ttatttttgc
    32761 cattctggtt tagtccttta ataaataata tcaattaccg ctaattaggg tctgaaatgg
    32821 ggattcatcg agaaaattca agtaccttct ttattttttg aaacagccac catggcgggt
    32881 cgaatcactc gatcaaacaa cagatatccg cgttgcataa cctgggtaac agtattcgct
    32941 ggaagttccg actccaccat acacatcgct tgatgctgat gaggatcaaa tttctctccg
    33001 actggactta ttgctttaat attgaacttt tcaaaaacat tggtaagctg cttgtgcgtt
    33061 aactcaacac catttctaaa attttctaat gtgacatttt ccacagccag tgctgcatca
    33121 agactatcca taaccgcaag taattcaact gaaaattttt cgattgcata tttgtgtgcg
    33181 cttgcaatat ccgcttgagc acgtttacga atattctcgg catcggcttt ggcacgtagc
    33241 caagcatcat ggtgctcggc tgctttcagc tcagcatccc taagtaattg ttctaaactc
    33301 ggcgttgact caataaccga atcagaaaca gtatttcctg tgttactagc gctactcgtt
    33361 tctgtaccaa ccgaaataag ttcttgctcg cttgatgatg aattgtgcga ttcttgcata
    33421 tatcctctct gtaatttttc aggttatgtg gggcgcattt tgctaatttc aagttctttt
    33481 ctgaaaaatt tatcaaccct aatttctaca atggaaatct ttcgctaatt tttcagctaa
    33541 accaatataa tttgtaggcg tcaacgctag gagttgctgc ttctccttat caggaatttc
    33601 cagtgcttgg ataaattgat gtaaggtttc ctgagagatg ccagattttc cacgtgttaa
    33661 tgctttcaat cgttcatagg gattttttat cccaaaacga cgcattacca tctgaacagg
    33721 ctctgctaac acttcccagg tatcttgtaa gtcacgttgt atcgctatgt gattgatttc
    33781 aagtttatta agacctttaa tacacgagtc ataggcaagc aatgtatgac ccagtgcaac
    33841 ccccatattg cgtaataccg tcgaatccgt taaatctctt tgccagcgtg atacgggtaa
    33901 cttctcactc aaatgattca ataacgcatt cgcaatgccc agatttcctt cagaattctc
    33961 aaaatcaatc ggatttactt tatgcggcat tgtcgaggaa ccaacttcat tttccttggt
    34021 tttctgcttg aagtagccta atgaaatata gccccaaata tctcgattta aatcgatcaa
    34081 aactgtgttc acgcgtgcat aggcatcaaa taatgctgca attgcatcat ggggctctat
    34141 ctgagtcgtg tagggattaa atgtcagccc tagcttttcc acaaaatttt gagcaaactt
    34201 ttcccagtcc aagtttggat aagcaacgat atgtgcatta tagttcccca cagcgccatt
    34261 gattttcccc aatagagcaa tcgcccttag ccgatcacag gcataattca aacgataagc
    34321 gacattagcc atttccttac ccatcgttgt tggcgtagca ggctgtccgt gtgtacgact
    34381 caataatgga acactggcaa attgatgtgc catggtttgg agtttatcga ttagcgcatg
    34441 cagcataggt agcatcactt catcacggct atttttgagc atcaaaccat gggagaggtt
    34501 gttaatatct tcagaagtac acgcaaagtg aataaattca atcacatttg caatatctga
    34561 gtactgagaa aaagtttctt ttagccaata ttcaaccgct tttacatcat gattagtgct
    34621 tgcctcaatt gctttgatcg cttccgcatc ggttactgaa aaatttttta ttaaattttc
    34681 taatttacca acggtgttcg ctgagaacgg agaaacttca ccaatgagtg attcgttact
    34741 caaagcctta agccattcaa tttcgacgat aactcgatag cgaattaagg caaattcact
    34801 aaaatagggc cttaaagcag aagttttagc atggtagcga ccatccagcg gggaaagtgc
    34861 gcaaatggtt tgaaaagtca tgaaatatat cctgtttttc ctgctatttt atcttaatta
    34921 cacgataaga acgcggcaca aattaaagaa attaattcca tgactacagg attgatgaat
    34981 cgaactgatt cactcatgtg atgatattta ttgcagttgt cagtctattt ttttataact
    35041 tatccaataa aaatcgcttt gaccttaata gtgtctcatt tttgtttacg ccaaatccac
    35101 ataactcaat gttaattccg ctaaaacttc agtttattag cgagccatgc taggattacg
    35161 aataatctta ccatcaaaaa tagagagagg aatttgaatg ggtatccctt ttttacaaaa
    35221 ataccgagtg ggaagctaca tattaaaaca gaaaatttta ggtgttaagc gctatccgct
    35281 tgtattaatg cttgaacctt tatttcaatg caatcttgct tgtgctggct gtggaaaaat
    35341 cgactatcct gatgagacat taaaacgtcg tctaagtgtt caagaatgtt tggatgctgt
    35401 tgatgaatgc ggtgtaccag tcgtttcgat cgcaggtgga gaaccgctga ttcacaaaga
    35461 aatgccgcaa attgtagcgg gaataataca gcgcaagaaa tttgtgtact tatgtactaa
    35521 cgcgctactt ctggatgatc ggatggatga ttataagcct tccccctact tcacattctc
    35581 tattcacctg gatggtaaca aagaacgcca tgatacctct gtatgtcgtg atggggtata
    35641 cgacaaagtg attcccgtta tcaagaaagc gcttgagcgg ggatttagag tcactgtcaa
    35701 ctgcacgctg tttcagaatg agacagcgga agaagtcgcc gatttttttg ataccgcgac
    35761 tgagttaggc gttgaaggta ttaacgtttc cccaggattt agttatgagc atgctccgcg
    35821 gcaagatgtt ttccttcaac gttctgtcag taagcgttta tttcgtgcta tttttcaaga
    35881 aggtaaaaaa cgcaaacgca cttggaaatt taatcactca agtttgtatt tagattttct
    35941 ggcaggaaat caaagctatc aatgtactgc gtggggcaat ccaacccgta atttgttcgg
    36001 atggcaacgc ccttgttatc tgctcgtcga tgaagggtat gcctcaagct tccgtgagtt
    36061 gatggaagat actaattggg ataaatacgg cgtaggtaaa aatcctaaat gtgcaaactg
    36121 catggctcac tgcggctttg agccaactgc tatcaatgat acttttgcac atccaatcaa
    36181 agcaatgcgt gtcagcctaa atgggcctaa aactgatggt ccaatggcaa tcgatccatc
    36241 acaacaaggg agtactactg gcgcgagtaa tgcacagaag gattttccta tccctgtggt
    36301 tgtagagcca aaagcagtaa aagcttcttc ttcaggagat gtatcaaata aaaaataaat
    36361 aaccggtgga atttttattt cactacattt caaaacgtga aactagaact ctaccggata
    36421 cattgttatt cagattatgt ttgaaatttt taccgttggt tttgcttgct tcggtgcgct
    36481 tctttggtgg atcatcgtac tactgccttg gaagccaacg agcacaaaag aatatcttga
    36541 gtctaaccct cgattaaatt ctgaacaaat cagtaatgta acagttctca taccagcgcg
    36601 aaatgaaagt gcttcaattg aaagaacact acaagcgctg tcaacgcaag ggaaaaattt
    36661 accaatcatc atcgtcgatg atcaatctga agatgatacc agtgagaaag ctaaaaactt
    36721 tccagtaacc attatctcag gtacaacgct accaacggga tggagtggta agctatgggc
    36781 gctagaacaa ggattagccc atataaaaac atcttatgtg ttactgcttg acgctgatat
    36841 cgaactcgat aacggtatgg ttgcaacatt acttcagaaa gcagagcatg agcagttagc
    36901 gtttgtatcg ttaatggcag aacctaatat ggacagcttc atcgaacgtt tgctgatgcc
    36961 tgctttcgtt tttttcttta aactgctgta cccttttaag ctttccaata ccccccaatc
    37021 aaagatcgct gctgctgcag gtggttgcat tcttgtcaaa cgcgaagcgc tacatcaaat
    37081 cggcgccttt aattgccttc gaaatgcgct cattgatgac tgcacactag cgaatcgcgt
    37141 taaacaagcg ggttttctta cctggactgg attaagtcac tcagtcaaga gccatcgaga
    37201 gtatcagaca ttaatgccaa tttgggatat ggtggcacga acggcctaca cacagttaaa
    37261 ttattctatc ttttggcttc tggtgtgcac acttcttatg attagcctgt tctggtttgc
    37321 tccagttgga tcattcttag cggaagaatg gcttggcgtt atgattggtc tatttacttg
    37381 gttagccatg ttagttgctt atatacctac acttcagttc tatcgccgtt cattgctatg
    37441 ggtcgttgct ttaccgttta ttggtttttt attcctatta atgacctgga catctgcatt
    37501 gaaatattgg cgtggagaac gtgctttatg gaaaaatcgt cgctatgaaa tctcgttata
    37561 actttattct tttacgctat ctgatcatct tcagcgtggt actgtttgta cccgcactat
    37621 ctcattcaca accaaccgaa agcgaaacac cggagcaagt cattaatgaa ttacaagcta
    37681 cgctgattca agtaatgcaa aaaggcactg aattaggtta tcaagaacgc ttcaatcttc
    37741 tcgcaccagt agttcaacga tctcacgatt taccagcgat tattaaggct gtgcttggaa
    37801 cacactggaa taaattaacc gaggaacaac atcaagcaat taccgaaacg tttagtaagc
    37861 ttagcatcgc cacctatgca gagcgattta atcaacatga tcaagagcga tttgagttta
    37921 ttgaaaaacg taccttaccg aaagagcaag tacttgttcg aagccagctc atacaggctg
    37981 atggcaaagc agttaacttt gattatgtaa tgcgccaagg ggccagtcat cattggaaaa
    38041 ttatcaatat tctcgcggat ggtgtgagtg atctcgcatt aaaaagagca gaatacagtg
    38101 ccatcatcca acgcgatggc ttctctgcac tactttcctt gttagaacaa aagattattc
    38161 aggcagagca acatcactag tatcttctaa tacatgcaat attgttcact aacttagcct
    38221 ttacgtctgt gatcgtggtg cctgacttct catttactaa ttgagacaac tttcctttct
    38281 ttcagtataa cgaactcagt gaaacctacc gatcaacaag aacgtgcatt gatagcctgg
    38341 gcggcgtttt gctatcgcta cgctttttgg gttttgtgtt ttttactcat aaccgggatc
    38401 gcatgcggca cttataccgc gcgtaattta gaagtgctaa cggatacggc tgatatgtta
    38461 tccgaagaac ttccttttcg tgtcaacctt gccaaactca ataaagcttt tccacaatac
    38521 gaaaatacct tgattctggt gctcgatgca cccacacctg aacaagctca tgccgcagca
    38581 agccgattaa cgtcttattt aaaagacgga catatcgagg cttatgatat ttattatgga
    38641 gcgggagaac cattcctaga acagaatggc ctactttata aaaacatcgc agagcttgaa
    38701 tttttagcag accggcttgc ggcagcgcaa ccgctaattg ttagaatcgc tcaaaaccca
    38761 agcctattaa ccttcattca taccctgaca gaagctgtcg atgcatttcg tagcggcaac
    38821 aacctcgaat tagacccagt tctagacgga atttcagcaa cgttgtctgc aaggctcgcc
    38881 cataatccta ggccgctttc ttggcaagca ttatttaacg atgagaaccc tcaaagcagc
    38941 tacaaagaaa tcattatcct acaacccaaa ctagattaca ccgaactctt tgcagctaaa
    39001 gattcgattg atgcgataag agccgcattt cagacactta atcttgaaaa tgatttttta
    39061 acaaaactgc ggattacggg tgaagttgca ctttcctatg aagagttgct gagtgccatg
    39121 catggcgctg aatatgcggg tttggttgcg ctcatcatcg ttacgcttat tctttttgtt
    39181 gctttacgat cagcgggggc catcattacc gtgctagtga gcttactatt gggattagtc
    39241 atcacagcgg ctttcacgac ggcaacgatt ggtcatctca atttaatttc aatcgctttt
    39301 gctgcgctct atattggact tgcagtagat tacgccattc atttgatact ccgctaccac
    39361 gagttgaatc gaccagatca acccgtcatc gaaatattgc taagggctat tcacgatatt
    39421 ggcccttctc tgctaatttg cgcactcaca accgcaattg gattctatgc ttttattcca
    39481 acaacttttc taggaatcgc tgaactggga atcatatcgg gaactggtat gtttatcagc
    39541 ttattcataa cgttaacggt cgtccccgca ttacaacgat ttctgccctc caaaatttac
    39601 tctcaagtac gtaaaaatag atcaataact cagcaatggc tagcgcttcc acaaaaatgg
    39661 cctaaaacag tttatgtgtt agtagcacta tcttttgcac ttgcactcac cacatggaat
    39721 caaattagat ttgattataa tttactgaat ctcagcgacc ctaatggtga agctgtgtta
    39781 acttttcgcg agttactcgc cgatacgcaa cattcaccct ggctatcgat ggcaactgtc
    39841 aaaagtcttg aagaggctaa taaccttgct acgcagttaa caaacctacc tgtcgtcgat
    39901 aaagtgatta gcatcgctga tttcatccca gatcaacaag atgagaaact tcaaatcatt
    39961 gaaggaattg cactcacact cggacctacc ttctttactg aaaaccatgt cgaaaatgat
    40021 gatcttgagt ccagtgattt cacccaaatt aaccaagcgc ttgaaaattt aaggtatcag
    40081 ctcaatcaat taatgattga aaaacctaat cacttaagta ttgagtcatg gaaaagactg
    40141 agcttttcga ttgatcaact acttaccttt atccacacag ttcctgctgg acaagtgcaa
    40201 cctttcttaa atgcacttca aaatgattta atcggtcaac tgcccggtgt actcaatcgc
    40261 ctccagcttg ctcttgaagc tagcaccatc aaggatgaat ctttaccaag ctcattacgc
    40321 cagcgctggc acaatgaatc cgacgatttc cttgttgctg tttacccatc agaaaacctc
    40381 aatgataacg atgcactgaa acgtttcatt cgatcggttc aacaacatgc accacaaatt
    40441 agtggcacac cagccatcat gctcgcagca ggagaagcgg tggttgacgc atttatccat
    40501 gcattcattc ttactttact tggtgtaacc ctatcacttt atgttttatt gcgaaaaaac
    40561 attttacttg ttgtaataac gcttctgcca ttacttctgg cagcattatt tacagttgca
    40621 acaacagcgc tgctaaacat tccatttaat ttcgctaatg taattgcatt accactttta
    40681 ctaggcattg gtatcgatag tagcatccat atggtttatc gaagcttgag ttctgtagaa
    40741 aaccaaagaa gcttgctaca aacgagtaca ggctctgcca tattttatag cgcactgacg
    40801 actatggcgg gatttggtag tttgattcta tctactcatc agggaacagc aagcatgggt
    40861 atgctgttag ccgttggaat tctttacatc ttgatttgcg tctttatcat tctacctacc
    40921 ctgcttactc gttttaaaca taagcttaat ttctaaatac tgcgacaaat aatcagctaa
    40981 gggcttcaag ggcctctatc atcattccag caacattaaa tccagtttgc ttggttattt
    41041 ctcgcatccc agtcggactc gtcacattga tttcagttaa tgaatccccg atcacatcaa
    41101 gcccaaccaa tattaagcct agcgtattta ggaccgggcc tatcgtagtt gcaatttgta
    41161 aatcacgctc tgacaaaggc tgaacaatac caattccacc cgcagccaga tttccccggc
    41221 tttctcctga ttttggctta cgtgcaagtg catagggaac cggtttcccg gcaataagca
    41281 aaattcgttt gtcacccaat acaatctctg ggagaaaacg ctggaccata atggtacgcg
    41341 tcccataatg agtcatcatt tccagaatta cgctcaagtt aggatcagta ctatgcacac
    41401 gaaaaatact agcgcctccc atcccatcta acggtttcaa tacgatatct tgatgttgac
    41461 tcagaaattc ccgaatcaaa tgctcatttc tagttactaa cgtggcagga ataaaatcgg
    41521 gaaattttgt aattgccagt ttctcattat gatctctaac actcctaggg ttattaatga
    41581 ccaacacacc ctgttgctgt gcgatttcta gcaaataggt gctataaata tactccatat
    41641 cgaaaggggg atctttacgc atcaagacta tatcgaattg tgctaatgga acgacttctt
    41701 tggggcctag atcaaaccaa tgtgcccgaa tctctcccga ttgcttgggc tgcaactcgt
    41761 ttactctggc catgacgata ttattttcat aactcaggtc accttgatgc attacaaaaa
    41821 cccgatgatc acgcacgatg gcttcttgca tcatcgcaat actagagtct ttttcaattt
    41881 tgagcgattc taacggatca ataataaaaa ccaaattcat acgaaatcag ccataacttg
    41941 caatcgacct aagcgccaac cttaggtgca tgctgctcta gttcaacggc agcggcaagt
    42001 aaagccagcc tcgctacaac accataagca tagaaccgat tgggaactga atcaggaagc
    42061 gctgcatgat tgggaagtac acaggaagta tcaaatgcta atggaacaaa atgcatacca
    42121 ggtgcgttca aattctcatc gataccttta ccggtatgaa cacgatagaa tcctcctaca
    42181 acataacgat cgatcatata gatcacgggc tcggcaacgg cttgatggat gctctcaaaa
    42241 gtataaacac cctcctgtac caaaacgtgg ctcacagaca gcccttcttt gattttagcc
    42301 attttattgc gctgcttgcg attaagctta tagacatcag ccccatcttt taccgtcatg
    42361 atccccatgc cataggtacc tgcatccgct ttgacaatta caaagggctc ttccttgaca
    42421 ccatattgag tatatttttc tttaatatta ctgagtattt catctacggt tgctgcgaca
    42481 caatccaccc ctttctcctc ataaaagtcg atttttccac aagaattaaa atagggatta
    42541 ataagccatg tatcgatacc aatcagttcc gcaaactcct gtgctacccg atcataagcc
    42601 gtaaaatgct gagactttaa gcgcgtagtc catcctgcat gaattggagg aataataatt
    42661 tgatctaaat tttttaagtt atcaggaatt ccacttgaaa gatcattatt taacaaaata
    42721 gcgcagggat caaagtttcc aaccgataga cgatttcctt tccttacgat tggctctaga
    42781 agtagcgtat ttccatcagg tagattaata ggtgtaggtt ctgtaatttc cggaatcaac
    42841 gaaccaattc ggatatttaa tcctgcatgt cgcataatcc gctctaatac atgcacattc
    42901 tgaagataat aaatatttcg tgtgtgactc tctggaatga tgagaatact atgcgcttca
    42961 gggcaaattt tctctactgc cgccatcatc gcttgtacac aaagtggtaa aaagtcggtg
    43021 ttgagattat taaaaccacc cgggaacaga ttggtgtcga ctggcgctag tttaaaccca
    43081 ctatttctta aatcaactga acagtaaaaa ggaatagtat gttgctgcca ttttcctcgt
    43141 aaccagtgct caattgcggg cattgcttca agaatacgct tttcaagcgc gaggatcggt
    43201 ccacttaagg ccgtagtaag atgtggtact gccatcaaag caaatcagaa tgattgaatt
    43261 agagattata tcatgctaat tggctttacc cgctaatgcc atcattacaa aaaaaaacga
    43321 cccctaaaaa ggggtcgttt tctctaaata agctaaactg cttctttatg aattaatgat
    43381 ccatcgctgc atctttgtta aagcttgcag gttttttgag ttgttccatg atgtcattat
    43441 cccattcacc ttcaacattc atatgagctg ctgcgccaag caatacagct tcaattaagt
    43501 tgtggctcag gtatacatag agaccaggca ttttgaaatc ataaagtgct gcaacagctg
    43561 ctccaccagg tacaaaccat gtttcttggt tagtaattgg aggatcatta aatgaaccac
    43621 cttgccatac taagtcacca tgaccaccaa tcagatgtgg acgtgaatca cggttagctt
    43681 gtgagtggat aaataatact ttttcgccca ctttagcttt caatgcattg tcaccggtta
    43741 tagctccaac agcaccgtta aacactacgt gacttggtat aaggcctgca gctactccta
    43801 agatatcagc catacctgca gcaggttgat cataggattt gtatttacca tccgcacctt
    43861 taggtacata gaaatcttgc tcaccgatat aatatgcacg atcgtattta actgatttac
    43921 ctttattgtc tttcagacca tcttttggta ataccataat cgcaccactc ataccagaga
    43981 taacgtggaa tggaatcatc gttccacctg gcgcgcaatg atagacaaag acgccatttt
    44041 tgagtgcttt ccagcgcaat accacttctt caccaggttg aaccaatgta agttgagcac
    44101 cgcctaatgc acctgttgct gcatggaaat caacgttatg tgccattgtg ctcgttttag
    44161 ggttttttaa tgtaagctct acataatccc cttcatgaac aacgataagc ggaccaggaa
    44221 ccgtaccatt aaaagtcaat gcccagtatt taacaccagg agcaatttca atttgctttt
    44281 caatggtttc catcgttact tcaaccactt tagggccttt tgaaacttga tcatgctcag
    44341 gaacaaacgg tggtgcaaca agtgtttgtt ttacacgtgg caattttgaa atatcatgct
    44401 ttgttgcgaa tgcaacagaa gcagtaaaac agagcagccc tagcccaatc actgcttgta
    44461 cagctttatt attcatatat ccctccataa attttaaaaa ttgcgcaatg ttatctaatc
    44521 agaagcgcca ttcttttttc ggtactgctt cccacattac cgcaaccgca taaactccaa
    44581 ctatcacaaa caaaaaacag aagcaaatgc agcgtgttaa ctatacactt taatcactag
    44641 cctgtccacg aataaccttt ctagcactag ggaatgcgcc ctttattcct atcattgata
    44701 tcaatttatc atcctatcaa gaaaccattt acctttaaaa ataagggtaa tatttaaggt
    44761 atatagctat catctcgaac ggtgtagtaa agcctttttt gcagcctcat attcttcttg
    44821 agtaattgca ttttctgcca gtgcacgctg cagatcttgc agttcctgcc ctgtcgttat
    44881 cgtggaagtt ccttgcactt ttacagatgc atctcctcca aaagcgcaac tggtaattag
    44941 cgtaattaat gccaaaacta aaactgtgaa ccttattgcc atcttgaatt ttctcccaat
    45001 ttttagcgaa tactatcaat tagtcgagct ttttggcgct gaaattcttt ctcagtcaat
    45061 gcgcctgaat cgtaagcttt ttttagatcg ataaactgtt gaccaatcga aacattcaaa
    45121 ttgggctgtt gtacaacttc agtgctacta catccgctta tcactagcaa aaaacacata
    45181 ttaactgcag tgattcctta acttactcac tatttctctc cgtttaataa tgatagataa
    45241 accctaattt agacccttta tcatttagcc aaacgattgc aaccttcctt ctcatagttt
    45301 aactaatatg gtctagacta ataagatctt gtctgctagc taggccttaa tattatgagc
    45361 ttagtgtgcc gtggtctcca gatccaatct aattttttct aatcgctcat cattcggaag
    45421 tacacttcca gctctttcca aatagagctg tgcatcttcc aacctacttt cgagtcttgc
    45481 tttagctatg aactgaatat agcgatcgac gattctcctt aatcctgcct gggcgatatc
    45541 attatgtgca tcaatagcaa gcaccatctg ataatgctta aacgcattat ctttagcagg
    45601 cgttgtaaaa cgcgaagctc tcatcgcttt ctcagcagcg tctaaatgca tcttgataac
    45661 attcttatca atcggttctt ctacaatcat ttgtgcagat gtgttcactg ataaatcagc
    45721 catggttgat tgctgatttc ctttcaattc tgattcaact gttgaacgct ctactttatg
    45781 atcttcacgt actaaaggaa tatctggtaa tgcggatac
//
LOCUS       DAOQLJ010000002        44064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_238_length_44064_cov_6.195665, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000002 DAOQLJ010000000
VERSION     DAOQLJ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 44064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44064
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_238_length_44064_cov_6.195665"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..235)
                     /gene="rpmF"
                     /locus_tag="PK522_00205"
     CDS             complement(53..235)
                     /gene="rpmF"
                     /locus_tag="PK522_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNC40183.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDFLTNPPLAIEPTTGETHLRHHISP
                     SGYYRGRKVIKTKNDE"
     gene            complement(262..759)
                     /locus_tag="PK522_00210"
     CDS             complement(262..759)
                     /locus_tag="PK522_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HNC40184.1"
                     /translation="MENIFIIDPLSFAQNKRIRHGKIAGDKLDRLRELIDSDEGFAYV
                     IEGDTDKLARPTLRLQIKGKIAVQCQRCLDKLNHEVDIDTLLILARNARELELYDADN
                     TIDAVLALHELDMGALIEDEIILSLPISPRHQEGGCNKDMQSILKREHKESPFSSLVS
                     LKQTH"
     gene            831..1430
                     /locus_tag="PK522_00215"
     CDS             831..1430
                     /locus_tag="PK522_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HNC40185.1"
                     /translation="MNRSIITASKIILGSSSQYRRELLSRLKLPFEVVSPEVDESALP
                     FETPEVTAMRLTKKKASVIAQSYPDALIIAADQVAAMGNIHLGKPLSHAKAKIQLSMM
                     QGKEVNFYTALCLLNSRTNHLQEHITRYIVKFRPLSDQQIENYLTKEQPYHCAGSAKA
                     EGLGIALIEKMTGNDPNALIGLPLIALVDMLLNENVEVL"
     gene            1603..1938
                     /locus_tag="PK522_00220"
     CDS             1603..1938
                     /locus_tag="PK522_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40186.1"
                     /translation="MLTNTLGWSFNSKVYTHELEHLRYSELFTANPENHRELHRQLSD
                     NGEIDAATHLCLHAAGQYQPFYFNPIITAIMVLNSIEAILVNNPKLLSEIILEVFLRP
                     PISLNTSFF"
     gene            2133..3566
                     /locus_tag="PK522_00225"
     CDS             2133..3566
                     /locus_tag="PK522_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNC40187.1"
                     /translation="MGCIHLNRLRLNWLLPKQIIWLLLFSVISCVQPAKANSELFMND
                     LIKTTVTEQNKLNADDGKNITLINAINLTLLHNPELAALAKEIGALGGATLQAGLFRN
                     PELSFNVENPGNIQKLRGDINSPDSVAQEVVQQLTTIRIGQLLELGGKRMARVQAAQL
                     NEELAVRDYESRRIEIIARVANVFTEVLAGQERLKLSEETKLLAQNVVDKVVLRVQAG
                     KVPPIEETRAKIGLSTARIEWEQAQRDLASARKRLALMWGSTLPQFDQALGSLETSTP
                     PPDFHRLQELVMSSPMALRAMKNIEHRKALVEVEETRRIPNLTVSAGIVHHAQLGGTT
                     AVAGLMIPLPLFDRNQGNLKEAHVRVNKAEDEHQALELRLKTELTQSYESLTAAWNEI
                     NILRNEILPGAKSAFNVMRRGYELGKFGLLELLDSQRVLFQNQLLYVRALANYQRLMN
                     DIERLIAVPIDTISPSQTNDTQSGKNY"
     gene            3594..5093
                     /locus_tag="PK522_00230"
     CDS             3594..5093
                     /locus_tag="PK522_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC40188.1"
                     /translation="MNKARLIPILIVIIVGIVLGALILTVDKSPGNSPEQAADNKTMQ
                     LEPNQSNTGPRGGSYFSDNEFGLELTIFEQGVPPQFRIYLYEKGKALPPIAAKVSIAL
                     SRLGAPIQLIYFSPEADYLLGDQVINEPHSFELVITAEWNGKTMRWKMSQVEARLEMT
                     NEMLKTIGVEIQTAGPAMIQSTLRLPGEIVLNPDRMVQVVPRLPGVVIAVNFEIGQTV
                     KKGQVLAVVESQILADLHSQYIAAQKRLALAQTVYEREKLLWEEKISAKQDYLAAQLA
                     LNEAKIASDLSSEKLRALGVQPKVGLQAKDLTRYEILAPISGLIVTRTIALGAALKDD
                     VTIFTLADMASVWATITVYPKDLSLIKIGQKASIKATAFDVEGSGIVSYVSPLIGGQT
                     RTATARVILDNQDGRWHPGMFVNIELVSEEIEVPIAVPVQALQALRDWTVVFGRYGDY
                     LEARPLELGRSDGKMVEIIKGLSAGEQYATGNSFAIKADMEKAGTSHDH"
     gene            5132..8287
                     /locus_tag="PK522_00235"
     CDS             5132..8287
                     /locus_tag="PK522_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNC40189.1"
                     /translation="MFERILRLSIDHSGLILVAVLAMALLGMNNYLRLPIDAVPDITN
                     VQVQINTTAAGYSPLEAEQRITFPIEMAMSGLPNLEYTRSLSRYGLSQVTVIFKDGTD
                     IYLVRQLVSQRLQEIRTKLPIGVIPTMGPISTGLGEIFMWTVDAEPDARKVDGTSYTP
                     TDLREIEDWIIKPRMRMVKGITEVNSVGGYVKQFHVTPYPEKLISYGLTLQDLITALE
                     RNNINVGAGYIEKSGEQYLVRVPGQVANLTEIGEIILGSNQGVPIRIKNVADVLIGKE
                     LRTGAATQNGHEVVLGTAHMLIGENSRVVSQAAAEKLAEINRSLPPGITATPVYNRTT
                     LVDKTISTVSNNLLEGAALVIVVLFIALGNLRAALITALIIPLSMLFTISGMVASNVS
                     ANLLSLGALDFGIIVDGAVIIVENCIRRLALEQEKLGRELTTAERFEIVFDASKEVRR
                     ALLFGQVIIMVVYLPVFALSGVEGKMFHPMAFTVLLALLGAVFLSITFVPAAVALFLS
                     GKMMEKEGKAVLWAKKLYAPALDSAMNNKGLTVTIAVVIITLSLLLTTRMGSEFVPNL
                     DEEDIALHALRIPGTSLSTAIEMQNELEETIKRFSEVKRVFSKIGTAEIATDPMPPSV
                     ADIFIIIKPQSEWSGKYRSKEELIAAMEKAVREVPGNNFEFTQPIQMRFNELLSGVRA
                     DVAVKVFGDDLDVMLTIAERIEKIIKNVPGAADVRIEQITGLSVLSIQMDREKMARYG
                     LNGNDVQDAINIAIGGRTAGLIFEGDRRFELQVRLPESIRGDIESLKRLPIKLPSVLI
                     PAVGQNVQPALTPNYVTLAEIASLEIVKGPNQISRENGKRRVVITANVRGSDIGSFVT
                     EAEQQIINSVKLPSGYWLAWGGQFEQLILAAERLQIVIPLALGLVFFLLYMMLNSLRD
                     SLLVFSAVPLAITGGIVALWLRDIPLSISAGVGFIAMSGVAVLDGLVLISFIRDLRQQ
                     GLPLNDAIRTGSLLRLRPVLITTWVESLGFLPMAISTSTGAEVQRTLATVMIGSTVSQ
                     SLLSLLVLPVLYQLTHRNNRS"
     gene            8725..9156
                     /locus_tag="PK522_00240"
     CDS             8725..9156
                     /locus_tag="PK522_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40190.1"
                     /translation="MSENHRNKEIEPGKDSLLDKPAQARRRLLKKSAAIPVIMTLHSG
                     AALARTSNIGTPIPDINAAATGGSSGDILCVHPDSSNGGPLGDGRYDLGTAPMVSGNY
                     QDPDNLENQALECNTRGGIIISATAYTSLSGRGVLPDSSTL"
     gene            9165..9455
                     /locus_tag="PK522_00245"
     CDS             9165..9455
                     /locus_tag="PK522_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr-rel-A system PqqD family peptide chaperone"
                     /protein_id="HNC40191.1"
                     /translation="MEECWKTLNYGSLIIEEWDGEFTVFQPDAGKTHYLNEMSVQILI
                     YIENNASVALSTADICDYLVKKFQLQTDTAFPKQINKTLRRFDELGLVKQIS"
     gene            9472..10380
                     /locus_tag="PK522_00250"
     CDS             9472..10380
                     /locus_tag="PK522_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HprK-related kinase A"
                     /protein_id="HNC40192.1"
                     /translation="MIVEQIPVDQFIAQLSHSGIRVKLGPINVLIRTKVPQLAKQLHF
                     LYTYYDIVENEIVDIHTHVLPKFSFNNPFSSFVRFTVDGQSPFPPFPADQALAALEWG
                     INLVIAVRVNHLLMFHSAAVERNGSLILLPAWPGSGKTTLCTALIHRGYRLFSDEFGL
                     MDPISDFFTPIPRLMPLKNQSIQVIRDYLPEAVLGEEIYNTQKGTIAHVRPPRDHIFK
                     TDQVARAKWIIFPKWTANAPLKLEPLAQSEAFLLLASNSFNYEILGESAFRAVTRLIR
                     KCQCYQLVYSDLDSALAMFNKLTDEA"
     gene            10370..11458
                     /locus_tag="PK522_00255"
     CDS             10370..11458
                     /locus_tag="PK522_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNC40193.1"
                     /translation="MKLSLSSHHQLLLDALINPSTLTQYTYAQWELLLRLARKVKLLG
                     FLAVELERRKLLDTIPVRVVSQLRSSLIRAKKQQQLAFWELDRINWALKSIPIPIIVL
                     KGVAYQLAKLPTAEGRFFVDLDILVPKDDLDQVEKALLKEGWKHREISAYDEHYYRAW
                     SHEIPALIHQERETEIDIHHTISPITSKIKIDSRLLIDAAIASQSGHKILNPTDMALH
                     CVVNLFQNNDLVDNLRDLLDLHDQLQYFASHDSAFWNKLIERANQLRLGHSLYYGMQY
                     SRLIFQTAIPENTEQRLIQKPSVVSQLIMRQLVPLALFPLHPDKASKRAKFARFLLYL
                     RSHWIRMPFYLLIPHLLYKTMLRFKPGK"
     gene            11550..11930
                     /locus_tag="PK522_00260"
     CDS             11550..11930
                     /locus_tag="PK522_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40194.1"
                     /translation="MQKQYKIFVNQIIALLLALLPFGYIPQVQAQASCSGCLCPGDPC
                     RLCPLPAMTSDTVADDEPETCKKIRQEVPPIDSQPGSNEYFASLDQSTMSCVKNGGDV
                     IKNSRRNEIFTSRYYCKPDLSTRK"
     gene            complement(12003..13178)
                     /locus_tag="PK522_00265"
     CDS             complement(12003..13178)
                     /locus_tag="PK522_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HNC40195.1"
                     /translation="MKMHAATVHQNTPSDAIFDTHPEISLDLSHVEAVLKKGYKKPFL
                     IVDRALIRNKFRRFRAVMPRVHPHYAVKANPDPRVLATLIEEGAGFEIASIAELDLLL
                     SLGVPAAEIFYSNPIKSRAYVEYAAAKGVEWYVLDSVEELRKIVSIKPDAKLYLRIDT
                     PNIGSDWPLAGKFGSHLADVKEIISEAVKLKADLAGVTFHVGSQCRNPQNWRVGIERA
                     KSVFADMEQSGLKPRLLNIGGGYPVRHTKPIPSIEVIAEVINAAIEDLSPEIHIMAEP
                     GRYLVSDSAYFVCQVVGTATRNGKRWMYWDAGIFGGIIEVSEGLRYEILTDRKGKKIP
                     WTIAGPTCDSVDILMRDELLPEDLQEGDFIYIPNAGAYTTAYASNFNGFPLPDVIVI"
     gene            complement(13514..14302)
                     /locus_tag="PK522_00270"
     CDS             complement(13514..14302)
                     /locus_tag="PK522_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNC40196.1"
                     /translation="MILTWIILAGLAGGMLSVLFAAFLTLKTSSAWVSTLVSYAIGAL
                     LGAAFINTLPEALAIADDSRSITIIILMGILAFFILEKLVLWRHCHLDECEAHDINQH
                     TMVNIQHDHGRSGIMITLGDIFHNFVDGVLIAAAFLVDVQTGIVTSIAIIAHEIPQEA
                     GDFIILLNSGYSRLQAILLNLLASTATLVGGVLAYFMLNQLNYLIVPMLGIATASMIY
                     VAMADLIPSLHKRPAISATIQQVVLIMMGIGSIWLIEVLFSHHH"
     gene            complement(14452..15210)
                     /gene="pgeF"
                     /locus_tag="PK522_00275"
     CDS             complement(14452..15210)
                     /gene="pgeF"
                     /locus_tag="PK522_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNC40197.1"
                     /translation="MKDWITPEWPSPANVRALFTTRQGGVSQDVEGKYAGLNLGDHVE
                     DDHALVQQNRQLLRNYLPSDPCWLKQIHGSRFVWVDALKSEHHDFEADAALSRTQGVV
                     CAVLVADCLPILLCNRGGSIVGAIHAGWRGLASGVIENAVYGMNCKTSDLIAWLGPAI
                     SNQYFEVGEEVRDTFVNQDGQSSLAFFAATDTGKWFADIFMLAKQRLVNLGINQIYGG
                     GECTYSNPSQFYSYRRDKRTGRMAALIWLSALNV"
     gene            complement(15280..16464)
                     /locus_tag="PK522_00280"
     CDS             complement(15280..16464)
                     /locus_tag="PK522_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC40198.1"
                     /translation="MSLLEKLDQAAAARKAQLPGGIGAFGIPIEAIYSATEARIDGRK
                     ILLFGTNNYLGLSFSSECREAAHAAIDEEGTGTTGSRMANGNYSGHRALEAEFADFYH
                     CRSSIVFTTGYQANLGTISALLGKGDVALIDGDSHASIYDGCILSGADIIRFRHNDKD
                     DLEKRLRRLGERSKNTLIIAEGIYSMLGDQAPLVDIVNLKKQYGSLLLLDEAHSLGVL
                     GETGQGLVEKTGLNHEVDFITGTFSKSLCGIGGYCVSNHEQLDQMRYVSHPYIFTASP
                     SPATIAATRAALKILRTATGLRKALWQNAEQLYSALKDIGYQLGPQPGPIVAIILDNP
                     EQALALWNGLMTANIYVNLILPPAAPEGKSLVRCSINAAHTPEQIHYVSQVLSELYAK
                     LH"
     gene            complement(16461..16718)
                     /locus_tag="PK522_00285"
     CDS             complement(16461..16718)
                     /locus_tag="PK522_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNC40199.1"
                     /translation="MNEVTQEGILKLLCQRLTGLTKADFEITSETNLITQLAIDSIKL
                     LNLIMEIEDNFDISVPVNALTDVITVRDLADLIYRIKSESK"
     gene            complement(16723..17655)
                     /locus_tag="PK522_00290"
     CDS             complement(16723..17655)
                     /locus_tag="PK522_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC40200.1"
                     /translation="MHQVVAVTGATGFIGQNLLDALIHDGWLVNALTRKQPSKNHHKN
                     IKWVLGDLDDHTALLELVTRASAIIHCAGLVRGNSPEVFFHANAIGTENLLKAIASVK
                     LNPRFLLISSLAAREPTLSWYAESKSKAEQIALHYLHDIGISYAIFRPSAVYGPGDKE
                     LLPVFQASRYGIVPVVGNFENRFGLLHVSDLVELICYWLNNESFTNGTYEIDDATPGG
                     YSYQSVAEIAQQILHRPVRCIKLPGKLIQQIASMNLWLSKFLNYSPMLTPGKFRELQH
                     PNWVCDMSLFKQKGINWAPHNSLREALPNLIKSK"
     gene            complement(17662..19500)
                     /locus_tag="PK522_00295"
     CDS             complement(17662..19500)
                     /locus_tag="PK522_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HNC40201.1"
                     /translation="MSSTIIKLGDPTMQESDFHPSDEVSVKMIPTPTLNELSQRLADF
                     ETLTDALEYAAKGVTGYNFYDAKGGLRSVLPYRTLKVNAQSKARRLLSLGFSHGSRVA
                     LIADTSPEFIELFFACRYAGLVPFAMPIPVNLGSHSIYVRQLQGMLESSGASAAIANL
                     DYLSFLKEAGDGLSNGHLKWVGTSAQLDSLPNSDKALKSNTPSETAYLQFTSGSTRMP
                     RGVVITEEALMCNLRGIVRNGLDVNPGDRCASWLPFYHDMGLVGLVLSPMAAQLTVDY
                     LATRDFAIRPLQWLKLISRNRCTIAFSQPFGLKLCALRVRDSDLEELDLSCWRAAGIG
                     AEMIRPEILRNFADKFASAGFSDRAFLPCYGLAESTLAVSFTKINRGIESLKVDTKTL
                     VEKRMAVRVQAEGRKFNEFVNCGRPLPEHTVKIVDENGQELPQMMVGSVLVKGGSIMT
                     GYFNNQEETDKALKSGGWLDTGDIGFLFDGDLYITGRKTDVIIVNGRNIRAQDIEELA
                     EQQSEVRSREASAFGISDSNGATTVVLVVECRLTSMEERQLLINRLQQLVYMAFGVNC
                     LVELVPPHTLPRTSSGKLSRFAARQGFLQRANMIDQLPTASLSGDR"
     gene            complement(19803..21302)
                     /locus_tag="PK522_00300"
     CDS             complement(19803..21302)
                     /locus_tag="PK522_00300"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNC40202.1"
                     /translation="MGELFDRLYIFEMANNHEGSVAHGLKIIDVVANLAKKHQIKAAV
                     KLQLRELETFIHPDYKQREDIKHIKRFESARLNFQDFEELVEAIHHHHLISVATPFDE
                     SSVANCQKLGIQILKLASCSATDWPLMTAMAEVKKPIIASTGGLGISDIDHLVSFFYK
                     RVPAFALMHCVSIYPTPNDRIHMNFLGKMIRRYPYVKVGYSGHESPENRMGVMAAVAK
                     GAQLLERHIGVSAEGIKLNRYSLDPDQADHWVSAAEEMREICGGDDHKFIGEDEIVSL
                     RELQRGVYVKQQVKQGTPILDDDVFFAMPCAEGQLTSGEFGQLRAKFIATQDYVPGAA
                     LYEKTEQDSIRMVREIVHDTKGLLYEAGIELGKDYQIEISHHYGLSKFREMGCVLINL
                     INREYCYKLLIMLPGQKHPPHLHQQKEETFYLLWGDITVNLRDSGEVSMKPGDRLLIH
                     RGILHDFMTKSGAIVAEISTHSIVGDSYYENKEIARLDPIQRKTFVESW"
     gene            complement(21360..22427)
                     /locus_tag="PK522_00305"
     CDS             complement(21360..22427)
                     /locus_tag="PK522_00305"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNC40203.1"
                     /translation="MLSHQKILVVVPARGGSKGVPLKNLRKIGGISLVERVGLLIQKL
                     DWIDRAVVSTDHPEIAKVAEGAGLAVPFWRPVELAGDQVGDIPVLQHALHATEAMDET
                     QYDIIVMLQPTSPLRRAEHVVATVEKLIAGNFDAVWTVSPTDLKYHPLKQLSLDDKGA
                     LNLWDKEGAHIVARQQLHQTYYRNGAAYALTRDCLLNQASLLGAHSFAVIIPEFMLSI
                     DTEEDFAIVEKYLAMRENQHDLNLNNAVINKTHSLGSKVFVIDIDGVIASLIANNDYA
                     LCQPISKNIEMINALYRAGHRIVLFTARGSMTGIDWSEITRQQLTDWQVLHHDLKFGK
                     PAADFYIDDRMILLDETSRFI"
     gene            complement(22448..23533)
                     /locus_tag="PK522_00310"
     CDS             complement(22448..23533)
                     /locus_tag="PK522_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNC40204.1"
                     /translation="MKILGAGSIGNHLAYACRQLGWNVTICDIDPAALRRTRDKIFPG
                     RYGKWDETIRLVQPAEVADEAFEVIIIGTPPDSHIDLALGCLAKTPPQVLLIEKPLCT
                     PLLQSCEQLIPIAKRVGTRVLVGYNHTLTSNTLLAEEWLQEGKLGKILTIDVQIREHW
                     QGIFKAHPWISGPEETYLGYTQRGGGALAEHSHGLAIWQHFARLSGNGRIIEVSAMLD
                     MVNEAGAYYDRLAQLSLRTETGLTGLLVQDVITEPAKKYLRILGSGGYLDWFINIKPG
                     ADAVKLWSQDDVERAQYFEKTRPDDFLPEINHIERILSNPALSSPISLEYGLETMLVI
                     AAAIKSHQERRVIKIDYTRPYGLESLT"
     gene            complement(23577..24962)
                     /locus_tag="PK522_00315"
     CDS             complement(23577..24962)
                     /locus_tag="PK522_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40205.1"
                     /translation="MSLNIAETVILPCETKSREFDAKLLLACFIAEKGIKAIVGAKKV
                     IDGLALSLPRGIYIGKSLTAKSATVLRMLRQLGYTVVAWDEEGLVWATPELYRLTKLD
                     SRTLKELVGLFAWGEANASAIREHPGFSNIPIYLTGNPRTDLLRPELRGLFTQEVEQL
                     IRRYGRFILINTNFSRVNHYYANESHLLNQLRRDENTNLVRNDLKIGLARHKKQLFDH
                     FIEVLPQLADHFPEINIIVRPHPSENHQAWYEAANHKQNVKIIYEGNVVPWIMAAETI
                     IHNSCTTAVESFLLDKIALAYCPVKSEVFDHALPNGLSVQCDNFQQLVEKVANSLQGN
                     SPMNSINRMQYHDLAKQHLASFERTFASERIAQILQELCKKPRRKVPLFSRMAGLGTA
                     YWRSFIKKRIEAVIPNHRNNTTYLRHLFPMTSLQEVENKVNQVSSLLGRFQHLHVNNI
                     AENVFQIEARH"
     gene            complement(25075..25491)
                     /locus_tag="PK522_00320"
     CDS             complement(25075..25491)
                     /locus_tag="PK522_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40206.1"
                     /translation="MFSMMVDIKSKSFVVIILVLLISGSFSEVNATHISRPILSEART
                     EYSQGEKIVVNGWVNYQDQSTPDVLLNAKLLRADGTEVIEVFGISDKQGKFSFEFETN
                     RLLPGSYQIIVTSQCLEVHRPVCTYQSSTLAIDMKE"
     gene            complement(26093..26755)
                     /locus_tag="PK522_00325"
     CDS             complement(26093..26755)
                     /locus_tag="PK522_00325"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNC40207.1"
                     /translation="MKLALFDLDNTLLAGDSDFQWAQFLIEQKVLDREVYEARNIEFY
                     EQYKVGTLDIHEFLDFQLKPLARHPREQLNTWREEFIQKKIRPIIAPGALELIAKHKL
                     ERNLCIIITATNSFVTSPIARILGVDHLIATEPEQKEGQFTGRVSGMPCFREGKITRL
                     EQWLDAHNLTWLSFLESWFYSDSLNDLPLLRKVTHPVAVDPDATLENHARQNDWPIIS
                     LR"
     gene            complement(26752..27435)
                     /gene="hda"
                     /locus_tag="PK522_00330"
     CDS             complement(26752..27435)
                     /gene="hda"
                     /locus_tag="PK522_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HNC40208.1"
                     /translation="MKQLLLDLSLENIPTLENFIPGRNVELLHALNEFLATSSSGNFI
                     YCWGGSGSGKSHLLKSVCDACIRLKRYQVAYFACDQYTEFELSDDLPGCIIVDDVDRL
                     GPEAQIKLFNLYNQARDDCLALFLVSGKTVPTQLQLRQDVITRLSWGLVYQIHELTDD
                     EKGKAMKNHAVNNGFELPAEVCDYLLHYEKRDMPYLIKMIDALGRYSLMLQRPITLPL
                     LRQFLNEVL"
     gene            complement(27586..28089)
                     /locus_tag="PK522_00335"
     CDS             complement(27586..28089)
                     /locus_tag="PK522_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HNC40209.1"
                     /translation="MSHSKILDGYRAIPRHDGIFKEEIHDTYQLKSKLAEPTVCSQCQ
                     AVYHQGRWQWLTAPASAHQGICPACHRIRDDYPAGYLTLQGVFYLEHREEIMHLVRNH
                     EKHQCTEHPLKRIMKIEESVDLTLVTTTDIHLVRGMGEAIHHAYQGDLEFRYNPEENR
                     LRVYWKR"
     gene            complement(28238..28855)
                     /locus_tag="PK522_00340"
     CDS             complement(28238..28855)
                     /locus_tag="PK522_00340"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HNC40210.1"
                     /translation="MIDKAPPRTITLAFTGASGMPYGIRLLEQLLAQHIRVYLLYSQV
                     AQIVAQQEMQLALPSRSQDAEHFFIDYFNVSKDLLKVFGREEWFAPVASGSNPAEAMV
                     VCPCTMGTLSAIASGLNQKLIERAADVMIKENRKLILVPREMPFSAIHLENMLRLARC
                     GVIILPANPGFYHHPKNIQDIVDFVVARILDHLGVEHSLIPRWGE"
     gene            complement(28852..29793)
                     /gene="hprK"
                     /locus_tag="PK522_00345"
     CDS             complement(28852..29793)
                     /gene="hprK"
                     /locus_tag="PK522_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNC40211.1"
                     /translation="MSQISITQLFEDNKEKLGLVWNLGLEYGSNHLSNDDTSCSSQGL
                     IGHLNFIHPNWIQILSNLTIDYLNQLDKASFQQKLDKLAQSSLACLVVADGATVPELL
                     QSFAIKTKIPLFCSSSSSLDVIWVLRSYLAKVLAPSTSKHGVLLDVLGMGVLITGDSG
                     VGKSELALELISRGHGLVADDVVEIHRIGPETLEGCCPPLLRDYLEVRGLGMLNIRTI
                     FGETAVRRRKNMKLIVHLEKTSAADIGSFERLPLSNLDEEILNVKIRKVTIPVAAGRN
                     LAVLVEAAVRNYILQLRGIDNTQEFIKRHEQEMAGDL"
     gene            complement(29780..30250)
                     /locus_tag="PK522_00350"
     CDS             complement(29780..30250)
                     /locus_tag="PK522_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNC40212.1"
                     /translation="MNLVSQFLPESNVIIDLDVASKKRVFEQAGLLFENTSHVARSQV
                     FDSLFAREKLGSTGLGQGVAIPHGRIKGLREATVALVRMKEAIPFDAPDGLPVNIACI
                     LLVPEKATDKHLQILSELAQMFSDKSFREKLLMSKNTKEIHQLITDWIPDNVPN"
     gene            complement(30345..30683)
                     /gene="raiA"
                     /locus_tag="PK522_00355"
     CDS             complement(30345..30683)
                     /gene="raiA"
                     /locus_tag="PK522_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNC40213.1"
                     /translation="MNLNLTGNHVEVTPAMREYVVSKISKITRHFDHVIDVSVILSVE
                     KHKQKAEANVHVRGKDIFVEADGADMYASIDSLVDKLDRQILKYKEKNMERRNHDTIK
                     KKEIDQEPEA"
     gene            complement(31104..31412)
                     /locus_tag="PK522_00360"
     CDS             complement(31104..31412)
                     /locus_tag="PK522_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HNC40214.1"
                     /translation="MLAVKLLFIDKEKEKSEKMTQSIIDCELHDYLEVACLYGYEVQL
                     TLKDQQIIQGKAKNIITTAEKREFLIVENATGLQQIELIQLQKLQVITPNAQFKEIIF
                     "
     gene            31504..32178
                     /locus_tag="PK522_00365"
     CDS             31504..32178
                     /locus_tag="PK522_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNC40215.1"
                     /translation="MKKIIKFVPLATIIMLSSCATISDTQRGTAQGAAIGAGAGAAIG
                     AIFGGGKGAAIGAGSGAVLGAGAGYIWSQRMEEQKKQMESATAGTGVQVSQTTDNRLK
                     LNIPSDISFDTGRDDIKSNFRPILDNFATSLLNNPGTSVMIVGHTDNTGTDAINNPLS
                     VNRAASTRDYLVSKGVPINRIQIDGRGSGEPVAANDTPANRAENRRVEIFVSEIQEEA
                     PPVSKP"
     gene            32297..33247
                     /locus_tag="PK522_00370"
     CDS             32297..33247
                     /locus_tag="PK522_00370"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNC40216.1"
                     /translation="MTELPNQKEGWEKELLEKVVLSTLREQRRTRLWSIFFRSLAFAY
                     LFILLFWGMGLFSDDSTSSSDRHTALIDVRGEIMADSLSSADNVNAGLQKAFKDKNTA
                     GVIIRINSPGGSPVQAGNINDEIRRLRTKHPQIPVYAVVEDICASGGYYIAVAADKIF
                     VDKASIVGSIGVVLDSFGFTGTLEKLGVERRLLTAGENKSFLDPFSPSDPKQREHARK
                     MLGEVHEQFIRIVQHGRGDRLKDSPEIFSGMVWTGQKSIDLGLTDELGNTEYVAREII
                     KAERIIDYTTKEGLAEHFAKRFGKVATGLFINTFSSLELR"
     gene            complement(33270..34166)
                     /gene="rfbD"
                     /locus_tag="PK522_00375"
     CDS             complement(33270..34166)
                     /gene="rfbD"
                     /locus_tag="PK522_00375"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNC40217.1"
                     /translation="MKILLFGKNGQVGWELQRSLAPLGRVIALDRFSQEYCGDITDTA
                     GITQIIRAIAPDVIVNAVAYTAVDKAESEPVLARQINTEATAVLAHEAKRAGAWLVHY
                     STDYVFNGSGNKPWLETDNPAPLNVYGQTKLEGEEAIRETGCLYLIFRTSWVYAVRGN
                     NFIKSILRLANERDQLTVINDQIGAPTGADYLADTTAHAIMMAINRPELSGLYHLVAE
                     GEISWHGYASFIINFAQAQGIDLKLKTNAIVPVPASNFKTPAKRPCNSRLSTDKFQKT
                     FRLNVPQWQIGVSRALTEILER"
     gene            complement(34172..35218)
                     /gene="rfbB"
                     /locus_tag="PK522_00380"
     CDS             complement(34172..35218)
                     /gene="rfbB"
                     /locus_tag="PK522_00380"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNC40218.1"
                     /translation="MILVTGGAGFIGANFILDWLSRIDEPVVNLDKLTYAGNLQNLIS
                     LQSDARHIFVQGDICDGQKLNQLFDNYQPRAVIHFAAESHVDRSIHGPDDFIQTNIVG
                     TFRLLEAARHYWNKLTGKAHYDFRFLHVSTDEVYGSLAPDVPAFTEKHPYSPNSPYSA
                     SKASSDHLVRAYHHTYGFPTLTTNCSNNYGPFQFPEKLIPLVIVNALAGKSLPVYGDG
                     LQIRDWLYVKDHCSAISRVLEAGVPGETYNIGGWNEKPNIEIIRLICDLLNEFCPGDY
                     HRLITYVTDRPGHDRRYAIDAQKIESELAWRPLETFETGIRKTVQWYLAHKDWVANVQ
                     SGEYRKWITTHYQQ"
     gene            complement(35244..36605)
                     /gene="tilS"
                     /locus_tag="PK522_00385"
     CDS             complement(35244..36605)
                     /gene="tilS"
                     /locus_tag="PK522_00385"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNC40219.1"
                     /translation="MGSIRKSSLNGIRDTVEVVLRQYVSEQNHLALALSGGVDSVVLL
                     QILAQISKQFTFNLSAIHVEHGISPNAKQWSDFCQSYCDALGVPLTVFHLSIKKQSQV
                     SLEAAARRGRYAIFKAISADFIVLAHHLDDQAETLQLQLLRGAGLRGLAGMPIMRSLG
                     SAANNVKLLRPLLTVSRDIIQDYALQNKLMWVTDESNDDTTYHRNFLRHQIFPVLEQR
                     YPAYRETLARTSRHIGEAIQLLDGLAENDSQSVMVMGDIHIERLRKLDFLRAKNLLRY
                     IFSRHDIIQPSTQKLEEILRQLWAVKSDNHLHLVFGEFEIRLYKNLVKLLPKAKPPDF
                     DQDQLFYWHGELNWLIEPLGGVIKWSNSTGTGINAKKLMENPVAVRLRKGGERFQPNC
                     RRPRRSLKKIFQEAAIPEWERNDLPLLLSGDQLVWVAGIGIDCGFQVMPGEQGLMPSW
                     HPS"
     gene            complement(36565..37542)
                     /locus_tag="PK522_00390"
     CDS             complement(36565..37542)
                     /locus_tag="PK522_00390"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNC40220.1"
                     /translation="MKTTFLDFEKGIEEFEEKIEQLRFAQDNSALDISAEIAKLQAKS
                     QALTKSVYAKLTPWQISQVARHPQRPYALDYIQHLFTDFEELHGDRNFADDHAIVGGL
                     ARFNGQSIMVIGHQKGRDTKEKIYRNFGMPKPEGYRKALRLMSLAEKFSIPLITFIDT
                     PGAYPGIDAEERGQSEAIGKNLYVMAGLEVPIVSVIIGEGGSGGALAVAVGDMVLMMQ
                     YATYSVISPEGCASILWKSAEKASEAAEIMGITADRLKEMSLIDGIINEPIGGAHRDY
                     PATMLSIKNVLQESLRKLQDISIESLLERRFNRLMGHGKYKEVKPERHT"
     gene            complement(37731..38336)
                     /locus_tag="PK522_00395"
     CDS             complement(37731..38336)
                     /locus_tag="PK522_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HNC40221.1"
                     /translation="MRISFDFGELKLRMTGRTQATALRAIYQLKVTLNYIRPPIWRRF
                     LIASTDSLANLHLVVQIVMGWSDSHLHEFAKGGERYGLPDEDFPSAIHDESKFRISQI
                     LKQEKDKIRYTYDFGDNWDHEIVLEKILPFEMGTKLPTCLKGSRACPPEDVGGPPGYE
                     MFLEALADPSHPEHGGILEWFGEPFDAEHFDLTEVNHLLHN"
     gene            complement(38475..39599)
                     /gene="earP"
                     /locus_tag="PK522_00400"
     CDS             complement(38475..39599)
                     /gene="earP"
                     /locus_tag="PK522_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HNC40222.1"
                     /translation="MSWDLFCRVIDNYGDIGFCWRLSRQLTTEYGLAVRLWVDDLASL
                     QRICPSVDPHQTFQQRCGVQINHWTDPFPDIIPADVVIEAYGCELPEKYIQAMLPSHS
                     RSRQPVWINLEYLSAESWVEEYHGLCSPHPRLPLTKYFFFPGFTANTGGLLREKDLLV
                     QRDSVQHNAANWWLQNDLPIPDETEIVISLFCYDTAPISNLLEAWAHSQTPIRCVIPE
                     GQALTLLRNGLNRSALKAGDVIQQDKLTVQIIPFLPQAQYDQLLWLCDCNFVRGEDSF
                     VRAQWAARPFVWQIYPQADNVHYVKLEAFLNRYSQSLTKESAQALRAFHHGWNGSERL
                     DWNHFWQHHAIFHQHALTWSSQLAVIPDLTFNLVEFCKRQ"
     gene            complement(39636..39884)
                     /locus_tag="PK522_00405"
     CDS             complement(39636..39884)
                     /locus_tag="PK522_00405"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HNC40223.1"
                     /translation="MSIAKVFEIISSSKVSFDDAIKKGVARAADTMTDITSAWVKDQS
                     VEISGGKIVEYRVVLKITFVLKPAPKAAVSKTKAKATK"
     gene            complement(40014..41114)
                     /locus_tag="PK522_00410"
     CDS             complement(40014..41114)
                     /locus_tag="PK522_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter Tol"
                     /protein_id="HNC40224.1"
                     /translation="MEKHVQSFKRSSWSIGFLSLALLCLAMSVTASDFIVEDHQLSDK
                     EVRVGEAYYRADMKWMVYQAEVSESNPFYQIYLKDLVSGKVQQVSPGDGKTTCAWVHP
                     SQEKVLFSSTHEDSDAKAKMLAEIERRKSGEKQSYAWDYDEYYDIYETDFTGGRIKNL
                     TNTLGYDAEASWSPDGKLIAFASNRRAYTEKLSDEEAVLLKKDPSAFIDIYIMNADGS
                     NVKRLTHTNVYDGGPFFSPDGKRIVWRRFSTNGREAEIFTMNIDGSDQKQITHLNMMS
                     WAPFYHPSGQYIIFSTNLHGHRNFELYIVDVDGKKQPVRVTDKDGFDGLPVFTPDGKA
                     ITWTSDRTPEKKGHLFHGKWDHTKALESLSLQ"
     gene            41263..>44064
                     /locus_tag="PK522_00415"
     CDS             41263..>44064
                     /locus_tag="PK522_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNC40225.1"
                     /translation="MKTETRLIVAICLTMIYSLFAMSASASDETFHHQMQITLIPSAS
                     EIQVTSTIQVPKHLLKSDSETKLDFSLHADLSITEMNGAKLSPQQKINTLLARPVPVK
                     RYTVTLPAQQNSFQLSFQGKIHHTVQGPGEEYARSFSYTPGLISEEGVFLANSTAWYP
                     QFENAMVSFQLEVKVPQDWDAVSQGTLLSEQKSSTTQQVVWEEKHPQDDIYLIAGRFE
                     RYTQPATTTITSYVYLRGSDKALAQKYLDTTAQYIAMYNKLLGPYPYTKFALVENFWE
                     TGYGMPSFTLLGPKVVRFPFILHTSYPHEILHNYWGNGVFVDYTQGNWSEGLTAYLAD
                     HLVAEQGRKGEEYRRDVLQKYTDFVNKEKDFPIKQFTSRHSSSSEAVGYGKTMMFFHM
                     LRQTLGDDDFVRLLRAFYQQYKFKQATFQDLLASINHYTRSDFTPFFAQWINQSGAPN
                     LHLQEAQAEPAANGFRLKVTLKQTQPGKPYDLTLPIAIHLEGQPQAYQHSIKLNQTVQ
                     TISIDLPQRPVRIDIDPQFDVFRRLDSREIPSALSQGFGAENALVILPSKADPALLQA
                     YQQLAANWQKTQSIRLEIIQDHQLDKLPVDRTIWVLGWENRFSQPVLSTLESQKVSYQ
                     ANTLQIAQQHYHQAEHAIVLTARQPENADKTLLWVAAHHPSAIAELARKLPHYRKYSF
                     LVFKGDELTNIDKGQWPITQSPLTQKIHQQDDPSFASDHVGSLSPRRTLAELPPLFSE
                     TRMLADITHLASATFKGRELGTPELDLAADYIAQQFHQAGLLPGGDNQRFFQVWQHDV
                     GPPKGIVSLRNVVGILPGSNPQLDKESLVIGAHYDHLGMGWPDVRHDNQGKIHYGADD
                     NASGIAIMLELARQIAPKWQPQRTIIFIAFTGEEANLLGSTYYANHDNNYPTKKIIAM
                     LNLDTVGRLGNNPVTIFGTG"
BASE COUNT        13228 a         9886 c         8312 g        12638 t
ORIGIN      
        1 atcaaaatac tattcaataa aaagataatg aaaaataagt ctctaaaaag ctttattcat
       61 cgtttttggt tttaattacc tttctaccac gatagtaacc agaaggactg atatgatgac
      121 gcaaatgcgt ttcacctgtt gtcggctcaa tagcaagtgg tggatttgtc aaaaagtcgt
      181 gtgaacgatg cataccacgc ttcgatggtg atttcttatt ctgttgaaca gccataaaat
      241 tactactccc aattaatttg attaatgtgt ttgtttcagt gatacaagcg aagaaaacgg
      301 actttcttta tgctcacgtt tcaagatcga ttgcatatct ttattacacc ccccctcctg
      361 atgccgtgga gaaataggta gacttaggat aatttcgtct tctattaatg cacccatgtc
      421 taactcatgc aaggctaaca ccgcatcaat cgtattgtct gcatcatata actcaagctc
      481 acgtgcattt ctcgccaaaa tcaatagcgt atcgatatca acctcatgat ttagcttatc
      541 aaggcagcgc tggcattgaa cagcaatttt tccttttatt tgcaacctca aagtaggtct
      601 agcaagttta tccgtatcgc cctcaataac gtaagcgaac ccttcatcag aatcgatcaa
      661 ttcacgtaga cgatctaact tatcccctgc aattttacca tgccgaattc tcttattctg
      721 agcaaaactt aaagggtcaa taataaaaat attttccata acattgcaat gctatatttt
      781 ttagaaatca gtgtcaaaaa caacgcatct atttataata agaattttct gtgaatcgtt
      841 caattatcac tgcttcgaaa attattttag gctctagctc tcagtatcgt cgagagctct
      901 tatcgcgatt aaaactacca tttgaggtag ttagcccgga agtcgatgaa agcgcattgc
      961 catttgaaac gccagaagta accgcaatgc gattgactaa aaagaaagca agcgtgattg
     1021 cccaatccta ccccgatgcg ctcattattg ctgctgatca agttgctgca atgggaaata
     1081 ttcacctagg aaaaccgtta agtcatgcta aagcaaaaat tcaacttagc atgatgcaag
     1141 gaaaagaagt gaatttctat accgcacttt gccttctaaa tagtcgaact aaccatttac
     1201 aagaacatat cacacgctat attgttaaat tccgccccct aagtgatcag cagattgaaa
     1261 attacttaac aaaggagcag ccctatcatt gtgctggcag cgctaaagca gaagggcttg
     1321 gcattgcatt gattgaaaaa atgactggga acgaccctaa tgcattaatt ggcctaccgt
     1381 tgattgcact cgttgacatg ttactgaatg aaaacgtcga agtactctga tttttgctat
     1441 agggttaaag aagtaccgct taaaaaaaat gatctagatt actcaaacgc tatactataa
     1501 taagcatcgc taaaaaaact agatcgatta aaataggtta tgtttcttat accttaccat
     1561 tcttatctaa taaagacggt ctgcttgctt ttcattataa ccatgctaac gaatacgtta
     1621 ggttggtcat ttaatagcaa ggtatacacc catgagctag agcatcttcg atatagcgag
     1681 ctgtttacag ccaatcctga gaatcaccgc gaattgcatc gtcaactttc tgataatggt
     1741 gaaatcgatg cggccaccca tctttgtctc catgccgccg gacaatacca acccttctac
     1801 ttcaatccaa ttatcacggc tatcatggtt cttaattcaa ttgaagccat actagtaaat
     1861 aaccctaaac tgctttcaga gataatcctt gaagtttttc tccgcccccc catctcatta
     1921 aatacatcat ttttctagtt tagttaaact ggttgatcta gttaaagttt ctacaaaata
     1981 cagctttgtg gtattgcgtc atcaatagga atattgattc tttccttcgc tgttagtaaa
     2041 aattagtagt attaccggtt aggtttcaac gcaactgaat tagatctgtt ggatccgcat
     2101 caagttaagt attttcatat tagagggata gcatgggttg tatacatctc aatcggctaa
     2161 ggttaaattg gttgctacct aagcaaatta tttggttgct gttattctca gtaatcagtt
     2221 gcgtacaacc agcaaaagcc aattctgagc tattcatgaa tgacctgata aaaaccacag
     2281 tgactgagca aaataagtta aatgcggatg atggtaaaaa tattacatta atcaacgcaa
     2341 tcaatttaac tttactacat aatccggaac tcgctgcgct agccaaggaa atcggcgctt
     2401 tagggggagc aacattacaa gcggggttat tcagaaatcc agaattgtca tttaatgttg
     2461 aaaatccggg aaacatccag aaactacgag gcgacattaa ctccccggat tcagttgctc
     2521 aagaagtcgt tcagcagctc actacaatca ggatagggca actacttgaa ttaggcggca
     2581 aacgaatggc cagagttcaa gctgctcaat tgaacgaaga gttagcagta agagattatg
     2641 aatccaggcg aatagagatt attgcaaggg tcgctaacgt gttcaccgaa gtattagcag
     2701 gccaagaacg tctgaaactg tcagaggaaa ctaaattact tgcacaaaat gtagtcgata
     2761 aagttgtatt aagagtacag gctggaaaag tccctcccat tgaagaaaca agagctaaga
     2821 taggtctctc gactgctcgc attgaatggg aacaagcaca acgcgattta gcttctgcac
     2881 gcaaaaggct tgcattaatg tggggtagta cgttgccaca atttgatcaa gcgctaggat
     2941 ctctagagac atccacgcct cctcctgact ttcatcgatt gcaagagctt gtcatgtcca
     3001 gtccaatggc gctccgtgcc atgaagaaca ttgaacaccg gaaagcactc gtagaagtag
     3061 aagaaacgcg ccgcataccc aatctcaccg tcagtgccgg tatcgtccat cacgcacaac
     3121 tgggtggaac cactgcggtt gcgggactca tgattccttt acccttattc gatcgtaatc
     3181 aaggtaattt aaaagaagca catgtccgtg ttaataaagc cgaagatgaa caccaagcgc
     3241 tagagcttcg actaaaaacc gagctcacac agtcctatga atctttgaca gctgcttgga
     3301 atgaaataaa tattttgcgg aatgaaatac tacctggcgc caaaagcgcc tttaacgtaa
     3361 tgcgcagagg ctatgagctt ggtaaattcg gcttattgga gctattggac tcacaacgag
     3421 tcctctttca aaaccagctc ctctatgttc gtgcgctagc caattatcag cgcctgatga
     3481 atgatatcga acgtctaatc gcagtaccaa tcgacaccat ctcgccttct caaacaaatg
     3541 atactcagtc tggcaaaaac tattaaagcg ctaccgaatt aaaaggaaaa attatgaata
     3601 aagcgcgatt gattccaatc ttaatcgtta ttatcgtggg catagtcctc ggagcattga
     3661 tcctaacagt cgataaatcc ccaggaaact cacccgagca agctgctgat aataaaacca
     3721 tgcaacttga acccaatcag tccaataccg gaccacgggg cggcagctac ttttccgaca
     3781 atgaatttgg tttggaactc actatttttg agcagggcgt tccgcctcaa ttccgaattt
     3841 atctttatga aaaaggtaaa gctctgcctc ccattgctgc aaaagtatct atcgcattat
     3901 cacgcctcgg tgcaccgata caactcattt acttttcacc tgaagcagat tacttattag
     3961 gtgatcaagt catcaacgaa ccccactcat ttgaactagt cattactgca gaatggaacg
     4021 gtaaaacaat gcggtggaaa atgagtcagg ttgaagctcg cttggaaatg accaatgaaa
     4081 tgttaaaaac gatcggggtt gaaatccaaa ctgcggggcc agcaatgatt caatctaccc
     4141 ttagattacc cggtgagatt gttcttaacc cagatcggat ggtgcaggtc gtaccacgcc
     4201 tacctggcgt tgttatcgca gtaaactttg aaattggaca gactgtaaag aaagggcaag
     4261 tacttgcagt cgtagagagt caaattctgg cagatctaca tagccaatat attgctgctc
     4321 agaaacgact tgcactggct caaactgtat atgaaaggga gaaattgctt tgggaagaga
     4381 aaatctcagc caagcaagat tatctagcag cacaacttgc actgaatgaa gccaaaattg
     4441 cttctgattt atcctcagaa aaactacgtg cattgggtgt gcagcctaaa gttggcttac
     4501 aagcaaaaga tctaactcga tatgaaattc ttgcacctat ttctggcctt attgtaacga
     4561 gaacgattgc actcggtgct gcgcttaaag acgatgtaac tattttcaca cttgctgata
     4621 tggcttcagt ttgggcaaca attacggttt atccaaaaga cttaagtctc ataaagatcg
     4681 gtcagaaagc ttcgatcaaa gcgactgcat ttgatgtaga aggcagcggt atcgtctctt
     4741 atgtttcccc cctcattgga gggcaaactc gtaccgccac agcccgtgtc attttagata
     4801 atcaagatgg ccgatggcat ccaggaatgt tcgtcaatat cgaacttgtt tctgaagaaa
     4861 ttgaagtgcc tattgccgtt ccggtacaag ccttacaagc cttgcgcgat tggacagtgg
     4921 tttttggtcg ctatggggac tatttagaag caaggcccct agagctcgga cgcagtgatg
     4981 gcaaaatggt agaaatcatc aaaggcttgt ctgccggtga gcaatatgca acaggcaata
     5041 gttttgccat taaggctgat atggaaaaag caggaaccag ccatgatcat taagccaatt
     5101 tttctggata gaaaactcac aaggaaaaaa tatgtttgag cgcattctac gcctatccat
     5161 tgatcatagt ggattgatcc ttgttgctgt attagccatg gcgctattgg gcatgaataa
     5221 ctacctcagg ctcccgattg atgccgtgcc cgatattacc aatgtacaag tgcaaatcaa
     5281 tacgacagcc gctggctatt ctccactaga agctgaacag cgcattacct ttccgatcga
     5341 gatggctatg tctgggctgc ctaaccttga atatactcgt tctctatcgc gttatggctt
     5401 atcgcaagtg acggtcattt ttaaagacgg aacggatatc tacctagtcc ggcaacttgt
     5461 tagtcaacgc ttacaagaaa ttagaacgaa gttaccaatc ggcgttattc cgaccatggg
     5521 ccccatttcc acagggctag gtgaaatttt catgtggacg gttgatgcgg aacccgatgc
     5581 acgcaaagtc gacgggacat catatacccc aaccgatcta cgagaaattg aagactggat
     5641 tatcaaacca cgtatgcgaa tggtgaaagg tatcaccgaa gtcaattcag ttggtggtta
     5701 cgtgaagcaa ttccatgtaa cgccctaccc tgagaaactg atttcttatg ggcttacctt
     5761 acaggatctc attaccgcac tggaacgaaa caatattaat gtaggcgcgg gctacatcga
     5821 aaaaagtggg gaacaatact tagttagagt acccggccaa gtggctaatt tgactgaaat
     5881 tggcgaaatc attctcggca gcaatcaagg tgtcccaatc cgtatcaaga atgtagcgga
     5941 tgtgttaatc ggcaaagaat tgcgaacggg tgcagccaca caaaacggcc acgaagtcgt
     6001 gctgggaaca gcccacatgc tcattggaga aaatagccgt gtggtttcgc aagctgcggc
     6061 cgaaaagctt gctgaaatca atcgcagttt gcctccgggt attactgcca cgcctgttta
     6121 caatcgcaca actttggtgg ataaaaccat tagcaccgtt tccaataatc tactagaagg
     6181 tgctgcgctt gtaatcgtgg ttttgtttat cgcactgggc aatctacgtg ctgcactcat
     6241 cactgctcta atcatcccac tttccatgct atttacgatc agcggtatgg tagccagcaa
     6301 tgtcagtgcc aatctactca gcttgggtgc gcttgacttc ggtattattg ttgatggtgc
     6361 agtcatcatt gtagaaaact gtattcgacg cctagcgcta gaacaagaaa aattagggcg
     6421 tgagctaact actgccgaac gctttgagat cgtttttgat gcttcaaaag aagtgcgacg
     6481 tgctttgctc tttgggcagg taattattat ggtggtctat ctacctgttt ttgcactttc
     6541 gggtgtggaa ggaaagatgt ttcatccaat ggccttcacc gttttattgg cgttactcgg
     6601 ggctgttttt ttgtcaatca catttgtacc tgctgcagta gcgctatttc tttctggcaa
     6661 aatgatggag aaagaaggta aggccgtact ttgggctaag aaactctacg caccagccct
     6721 cgatagtgcg atgaacaaca aaggactcac cgtaaccatc gctgtggtta tcatcacatt
     6781 gtctctatta ttgaccactc gtatgggtag cgagtttgtt cccaaccttg atgaagaaga
     6841 tatcgctctg cacgctcttc gtattcctgg aactagtctt agtacagcaa tcgaaatgca
     6901 aaacgagtta gaggaaacca tcaagcgatt ctcggaagta aagcgcgtat tttctaaaat
     6961 tgggacggct gaaatcgcta ccgatccgat gccgcccagc gttgctgata tttttatcat
     7021 catcaaaccc caatctgaat ggtctggcaa atatcgcagc aaagaagaac tcattgctgc
     7081 tatggaaaaa gccgtacgag aagtacccgg taataatttc gagtttacgc aaccgataca
     7141 aatgcgcttc aacgaattat tgtctggcgt gcgtgctgat gtagcggtaa aagtatttgg
     7201 tgatgacctt gatgtcatgt taaccatcgc cgagcggatt gagaaaatca ttaaaaatgt
     7261 ccccggtgct gcagatgtac gaattgaaca aattaccggt ttatcggtgc tttcgattca
     7321 aatggatcga gaaaaaatgg ctcgctatgg tctcaatgga aatgacgttc aagatgcaat
     7381 caatattgcg attggtggta gaactgcagg gcttattttc gaaggagatc gtcgcttcga
     7441 gttacaagtt cgactacccg agtcgattcg cggtgatatt gaatcactga aacgtttacc
     7501 gatcaagtta ccatcagttt taatcccagc cgttggccag aatgttcaac ctgcgcttac
     7561 acccaattac gtaacattag cggaaatagc cagcctagaa atcgttaagg ggccaaatca
     7621 aatcagtcgc gaaaacggaa aacgccgggt cgttattact gcaaatgttc gtggaagcga
     7681 tattggtagc tttgtaacag aagctgagca acaaattatc aatagcgtta agcttccttc
     7741 aggctattgg ttagcttggg gaggacaatt cgagcagcta atcttagctg cagagcgttt
     7801 gcaaattgtt attccactgg cgctcggcct tgtatttttt ctcttatata tgatgcttaa
     7861 tagcctcagg gacagtttgc tcgttttcag cgcggtgccg ctcgccatta ccggtggtat
     7921 tgttgcctta tggttgcgcg atattccatt atcgatttcg gccggtgtag gatttattgc
     7981 catgtcaggc gttgctgtat tagatggatt agtgctgata tcatttatcc gtgatctcag
     8041 acaacaagga cttccactta atgatgctat tcgaaccggc tctctactaa gactccgccc
     8101 cgttcttata acaacttggg tagaatcact tggtttcttg ccgatggcaa ttagcacaag
     8161 cacgggcgca gaagtacaga gaacacttgc aactgtaatg attggaagta ccgtctctca
     8221 gtcacttctt tcccttctcg tgctaccagt gctttatcaa cttacacatc gtaataatcg
     8281 cagttaattt tagtaaccag gtgatatagt ctgtcgaatt tctttacata tttttttatt
     8341 aactaactta aaggaaaaaa atatctttcc atccacttgt ttctatttga tttacaaaaa
     8401 tattctattg gatggcatgt atcttgcgta ttaaaaaaat aaagctttta ggttatcacc
     8461 ttaaattatg aagattcaat ttgattaaac ctagaaacaa ataaatatta atatatatta
     8521 atgagtatcc cactttgaaa agatttaact cttagagaat aagtgatcta agatactgat
     8581 aatctgtagg taatttgaaa tactcgatca tctttaagta agtgctataa tggctatcga
     8641 tagagattgt aaatagttag catttaaata ctttaatgcg cgtgtaaatc gaattcctcg
     8701 atagccatta ggagtttcgt aaatatgtct gaaaatcata gaaacaaaga aatagaacca
     8761 ggaaaggatt ctttgctgga taagccagca caagcaagaa gacgattact gaaaaaatcg
     8821 gctgcaatac cagtcataat gacactgcat agcggtgcag cacttgcgag aaccagtaat
     8881 atagggaccc ctattcctga tatcaatgca gctgctacgg gtggtagtag cggcgatatc
     8941 ctatgcgtac atcctgatag cagcaatggc ggcccactgg gtgatggccg ctatgattta
     9001 ggaactgcac caatggtcag tggaaattat caagatcctg ataatttaga aaatcaggcc
     9061 ttagagtgta atacaagagg tggtatcatt atttcagcta cggcttatac ttccttaagt
     9121 ggtagagggg ttttaccgga ctcaagcaca ctataagctc attcgtggaa gaatgttgga
     9181 aaacactaaa ctatggttct ttaattattg aagaatggga tggtgaattt actgtttttc
     9241 agccagatgc gggaaaaact cattatttga atgaaatgag tgttcagata ttaatatata
     9301 ttgagaacaa tgcttccgtt gctttatcaa cagcggatat atgtgattat ttagtcaaaa
     9361 aatttcaact acaaactgat actgcttttc ctaagcaaat caataaaacc ttgcgtcgct
     9421 ttgatgaatt aggtttagta aagcagattt cttaaagata aaacaattcg tgtgatcgtt
     9481 gagcagattc cggttgatca gttcatagct cagttatctc attcaggaat ccgagttaaa
     9541 ctgggtccta tcaatgttct aattcgaacc aaggttcccc agctagcaaa acagcttcat
     9601 tttctttata cctattacga tattgtcgaa aatgaaatcg ttgatattca cacgcatgtt
     9661 ctgcctaaat tttcatttaa caatcctttc tcatcattcg ttcgctttac cgtagatggc
     9721 caatcgccct ttccaccttt cccggctgat caggcattag ctgcgttaga atgggggatt
     9781 aatttggtta ttgcggtaag agtcaatcac ttgttaatgt ttcattctgc tgcggtggaa
     9841 agaaatgggt cgctaatact actacctgct tggccaggaa gtggcaaaac tacgctctgc
     9901 actgctctta ttcacagagg atatcggttg ttctcggatg agtttggttt aatggatccc
     9961 atttcagatt ttttcacacc catacctcga ttaatgccac tcaagaatca atctattcag
    10021 gtcattagag attatctacc agaagcggta ctaggtgagg aaatttacaa tactcagaaa
    10081 ggcaccattg cccatgttcg tccccctcgt gatcatatct ttaaaacaga tcaggttgct
    10141 agggcgaagt ggatcatctt cccaaaatgg actgcaaatg cgcctttaaa actggaacct
    10201 ctcgcacaat ctgaagcatt tttattactt gctagcaatt ctttcaacta tgaaatttta
    10261 ggcgaatcag cgttcagagc agtaactcgc cttattagaa agtgtcaatg ttatcagctt
    10321 gtttactctg atcttgatag cgcattagct atgttcaata aacttactga tgaagcttag
    10381 tttatctagt caccatcaac tgttactgga tgcgctcatt aatccaagta cattgactca
    10441 gtatacctat gcgcaatggg aattactatt acggttagca aggaaagtca aactattagg
    10501 ctttctcgcg gtggaattgg aaagaagaaa gctgctagat acaatcccgg taagggtggt
    10561 cagtcaatta cgttccagtt taattcgcgc aaagaagcaa caacagcttg cattctggga
    10621 gttggataga ataaattggg ctcttaaatc tattccgatc cccattatcg tattgaaagg
    10681 tgttgcttat caacttgcta aattgcctac tgctgagggg cgtttttttg tcgatctcga
    10741 tattttggtt cccaaagatg atctggatca agttgagaaa gcattattaa aagaaggatg
    10801 gaagcatcgc gaaatttcag cttatgacga acattattat cgagcttgga gccatgagat
    10861 tcccgcgctg attcaccaag aaagagaaac tgaaatcgat attcatcata cgatctcgcc
    10921 cattaccagc aaaatcaaaa ttgattcacg tttgcttatt gatgcagcca ttgcaagcca
    10981 atccggccac aaaattctta accctaccga tatggctcta cattgcgttg ttaatctatt
    11041 tcaaaataac gatttagtag ataacttacg cgatttactt gatttgcatg accaattgca
    11101 atacttcgca agccatgact cagcgttttg gaataaattg atagaacggg ccaatcagct
    11161 ccggttaggc cattcacttt actatggcat gcaatattct cgattgatat ttcaaacagc
    11221 aatccctgaa aatactgagc aacgcctaat tcagaaacct tcagtagttt cccagcttat
    11281 catgcgacaa ctggtcccat tggccttatt tccactccat cctgataagg cttctaagcg
    11341 agcaaaattt gcacgtttct tgctctacct gcggagccat tggatcagaa tgcctttcta
    11401 cttactcata ccccatctgt tatacaaaac gatgttgcga ttcaaaccag ggaaataaag
    11461 tgaacaacaa tcacttcacg tataatttta ctctctaagt ggaatagctg gtaaattgag
    11521 tattaaattc agataaaacg aggtattaaa tgcagaaaca atacaaaata tttgtgaatc
    11581 agatcatcgc gctattatta gcactacttc cgtttggcta tattcctcaa gttcaggccc
    11641 aggcttcatg cagtggatgc ctttgccctg gagacccatg ccgtttatgc cctttacccg
    11701 ctatgactag cgatactgtt gctgacgatg aacctgaaac ttgcaagaaa attcggcaag
    11761 aagtaccacc cattgactca caaccaggct ccaatgaata ttttgctagc ctcgatcagt
    11821 caacgatgtc gtgtgtaaaa aatgggggag atgtaatcaa aaactcgcgc cggaacgaga
    11881 tttttacttc acgctattac tgtaaacccg atttgagcac tagaaaatag tagtccaaac
    11941 ccactaaccc gttctagtga ttgattaaat ctactagaac gggtaggtat aacgcgtatc
    12001 acttaaatta caattacatc cggtaatgga aaaccattaa agttgctggc ataagccgta
    12061 gtataagcgc cagcatttgg tatataaata aaatcacctt cttggagatc ttccggtaat
    12121 aactcatcac gcatgagtat atcaaccgaa tcacaagttg gtccagcaat agtccaggga
    12181 atcttctttc ctttgcggtc tgtcaatatt tcataacgca atccctcact gacttcgatg
    12241 ataccgccaa atattcccgc atcccaatac atccagcgct taccattacg cgttgcagta
    12301 cccacaactt gacaaacaaa ataggcagaa tcagacacta aatatctgcc aggttctgcc
    12361 atgatatgaa tttcaggtga aagatcttca atggcagcat taataacttc agcaataacc
    12421 tcaatcgaag ggatgggttt agtatgtcta actggatacc ccccaccaat attaagtagc
    12481 cgaggtttaa gtccagattg ctccatgtca gcaaatacac ttttagcgcg ttcaattcct
    12541 actcgccaat tttgaggatt acgacattgt gagcctacat gaaaagttac acctgccaga
    12601 tctgctttta atttcacagc ctcactgata atttctttta catccgctaa atgagaacca
    12661 aattttccgg ccaggggcca atcactaccg atattgggtg tatcgattct caaataaagc
    12721 tttgcatcag gtttaatact aacaatttta cgtaattctt cgacgctatc cagcacgtac
    12781 cattcaacac ctttagctgc ggcgtactcg acataggctc tagatttaat cggattacta
    12841 taaaagattt ctgcagcagg cacgcctaag ctaagcaata aatcaagctc agcaattgat
    12901 gcaatttcaa aaccagcccc ctcttctatt aaggttgcta gaacacgggg atcagggttt
    12961 gccttgaccg cataatgagg atgcacacgc ggcattaccg ctctaaatcg tcggaattta
    13021 ttgcgaatca aagcgcgatc aacaattaaa aaaggttttt tatatccttt ttttaggacc
    13081 gcttcaacgt gggataaatc taggctgatt tctggatgcg tgtcaaaaat cgcatcagat
    13141 ggggtatttt gatgaacagt ggcagcatgc attttcatga ttttgatttc cttccaaaag
    13201 tcactacgtt tcaggaatat gaataaaaac taggtctgtt ggaatagaaa aaattacttt
    13261 gcttttcatg atgacctcct tacaaacact acaataatgg atatgttaaa gggacgaatt
    13321 atagtcttca aatttcaaga atcaagctct ttttaactaa agaaatagcg gcatttttaa
    13381 agcaagaata acttcgcgac tcgctccgtt ataaacgctc acgcagtgga ttactacatt
    13441 gcaagagagg ggaagaattt ttagggtatt caatcaggaa accgttaatg tagtctctga
    13501 tggtatcccc atcttagtga tgatggctaa ataaaacctc gataagccaa atagacccaa
    13561 tacccatcat aatcaaaacc acttgctgta tagttgcaga aattgctggg cgtttatgta
    13621 agctcgggat caagtcagcc atagctacat agatcatact tgcagttgca atgccaagca
    13681 tcggtacaat caaataatta agttgattga gcataaaata agcgagcacc cctcctacta
    13741 atgtagcagt actagccagc agattcaata atatggcctg cagccgacta tagcccgaat
    13801 tgagtaaaat aataaaatct cccgcttcct gcggaatctc atgggcaata atggctatcg
    13861 aggtaacaat ccccgtttga acatcaacta aaaaagcagc agcaatcaga acaccatcga
    13921 caaagttatg aaaaatatcg ccgagggtta tcatgattcc actacgacca tgatcatgtt
    13981 gaatattcac catcgtgtgc tgattaatat catgcgcctc acactcatct aaatggcaat
    14041 gccgccataa caccagtttt tctaaaataa agaatgccaa tattcccatt aatatgatga
    14101 tagtaatgga tcgtgaatca tctgcaattg ctaatgcctc agggagtgta ttaataaaag
    14161 ctgcgccaag taatgcaccg attgcataag aaacgagtgt tgatacccag gcactactcg
    14221 tttttaatgt caagaaggcc gcaaataaaa cgcttaacat tcctcccgcc aaaccagcca
    14281 agatgatcca agttaaaatc ataaaattat tcagaattat ctatcaaaaa aacaaaatcc
    14341 agcacgtaaa catccgagtg ctggattcat ctatacattc acgatcagaa ttaaatctta
    14401 atatagctta tttcagtcat gaccatctaa ccaaagtcag tgtatgccaa ctcaaacatt
    14461 tagcgctgac aaccatatta atgcagccat ccgtccagtt cttttatcac gtcgatagga
    14521 ataaaattgg gagggattac tataagtaca ttcaccccca ccatatattt gatttatccc
    14581 taaattaacc aatctttgtt ttgctagcat aaatatatct gcaaaccatt ttccagtatc
    14641 tgttgcagca aaaaaggcta agctggattg accatcctgg ttaacaaaag tgtctcttac
    14701 ttcttcaccc acttcaaaat actgattact aattgccgga ccaagccaag cgatgagatc
    14761 tgaagttttg caattcatcc cataaacggc attctcgatc actccacttg ctagccctcg
    14821 ccatcctgca tgaatagccc caacaatgct tcctccccga ttacatagca gtatcggtaa
    14881 acagtcggcc actaaaacag cacatacgac cccttgagtc cgcgataagg cagcatcggc
    14941 ttcaaaatca tgatgctcac ttttcaatgc atcaacccag acaaatctag atccatgtat
    15001 ctgtttaagc cagcatggat cgctcggaag ataatttctc aataattgcc ggttttgctg
    15061 aactagcgca tgatcatctt caacatgatc acctaaattc agcccagcgt acttcccctc
    15121 aacatcctga ctcacaccac cttgccgagt tgtaaatagc gctctcacgt tggcaggaga
    15181 tggccattct ggtgtaatcc aatctttcat acgagagaaa atctatctta tagacggata
    15241 gaaaacaatg aagtaattcc cgatatacgt aaaataaaac taatgcaact tagcatataa
    15301 ctctgataac acttgtgaaa cgtaatgaat ctgttcgggc gtatgggccg cattaatgct
    15361 acatcgaact aacgattttc cttcaggagc cgcagggggc aaaattaaat tcacataaat
    15421 atttgctgtc atgagaccat tccataaagc aagcgcttgc tctggattat cgagaataat
    15481 tgccacaata ggtcctggct gagggcctaa ctgatatcca atatctttta atgcgctata
    15541 gagctgttct gcattctgcc aaagcgcttt tcttaaccct gttgcagttc ttaaaatttt
    15601 taatgcggca cgagtagccg caatggttgc aggagaagga gatgccgtaa aaatataggg
    15661 atgactcaca tagcgcattt gatcaagctg ctcatgatta ctaacacaat atccgccaat
    15721 cccacagaga ctcttactga atgttccagt tataaaatca acttcatgat ttagtcctgt
    15781 tttctcgact aatccttgcc ctgtttctcc taacacccct aaggaatggg cctcatccaa
    15841 taaaagtaag ctgccatatt gtttttttaa attgacaatg tcgaccaggg gcgcttggtc
    15901 tcctaacata ctatagatac cctcagcaat aatgagcgta tttttacttc gttcccccag
    15961 gcgacgtaat cgtttctcta agtcatcttt atcattatgg cggaaacgga taatatctgc
    16021 accactgagt atacaaccat cgtaaatact ggcatgtgaa tcgccatcga tgagcgcaac
    16081 atcgcctttt cctaataatg ctgaaatagt tcctagattc gcttgataac ccgtagtaaa
    16141 aacgatactg gaacggcaat gataaaaatc tgcgaactca gcttcaagcg ctcgatgtcc
    16201 agaataatta ccattggcca tccgggatcc cgtcgttcca gttccttctt catcaatcgc
    16261 tgcatgagca gcttctcggc attctgatga aaaactcagc cctaggtaat tattggttcc
    16321 aaataacaat atcttgcgac cgtcaatacg cgcttccgtc gcggaataaa tggcttctat
    16381 cggtatacca aatgcaccaa tcccgcctgg caattgtgct tttctagctg cagcagcttg
    16441 atcgagcttt tctaacagac tcatttcgac tccgatttaa ttcgataaat caaatccgct
    16501 aagtctcgaa cagttattac atcggtaagt gcattaacag gaactgaaat atcaaaatta
    16561 tcctcaattt ccataatcaa atttaatagc tttatcgaat ctattgccaa ttgcgtaatg
    16621 agattagttt ctgatgttat ctcgaagtct gctttagtca gaccggttaa tcgctggcat
    16681 aatagcttta ggattccttc ttgcgtcact tcattcatag ttctatttag attttattaa
    16741 attggggagc gcttcgcgaa gcgaattatg gggagcccag ttgattccct tctgcttaaa
    16801 caaactcata tcacataccc agttcggatg ctgtaattca cgaaatttac ctggtgtcag
    16861 catgggcgaa taattcagaa acttagatag ccataaattc atgcttgcaa tctgctgtat
    16921 taactttcca ggtaatttaa tacacctaac cggtctatgg agaatctgct gagcgatttc
    16981 tgcgacagat tggtaactat atccgccagg tgttgcatcg tcaatctcgt aagttccatt
    17041 cgtaaagctt tcattattta accaataaca aataagctcg actaaatcac tgacatgtag
    17101 caaaccaaac cgattctcga aattaccaac tacaggtacg ataccatagc gagatgcctg
    17161 gaataccggt aacaactctt tatcaccggg tccgtaaaca gctgatggtc gaaaaatagc
    17221 atatgaaata ccgatatcgt gcaaataatg aagcgcgatc tgttctgctt tagatttact
    17281 ctctgcatac catgacaatg tcggttctct tgcagcaagg gatgaaatca ggagaaaacg
    17341 agggttaagc tttactgatg ctatagcttt cagtaaattc tcagtaccta tcgcattagc
    17401 gtggaagaaa acctctggcg agtttcccct aaccaaacca gcacaatgaa taattgcaga
    17461 agctctcgta acaagctcta gaagcgcagt gtgatcatca agatcaccca aaacccattt
    17521 gatattttta tgatgatttt tagatggttg ctttctagtg agtgcgttta ctaaccaccc
    17581 atcatggatt aatgcatcaa gtagattctg cccaataaac cctgtggcac ctgtaaccgc
    17641 cactacttga tgcatttaga attatctatc tccagataat gaagctgttg gtaattgatc
    17701 gatcatattc gcacgctgca agaatccctg gcgtgctgca aatcgagaca atttacctga
    17761 tgaagtccta ggcagtgtat gaggaggaac cagctcaact aagcaattaa ccccaaatgc
    17821 catatacact aactgttgta aacgatttat cagtaattgt cgttcttcca tcgaagtgag
    17881 acgacattca acgactaata caacagttgt tgcgccattt gagtcactga taccaaatgc
    17941 cgatgcttcg cgcgatctaa cttctgattg ttgttcagct aattcttcaa tatcttgagc
    18001 acgtatattt ctaccgttaa cgataataac atccgttttg cgtcctgtta tatagagatc
    18061 gccatcaaat aaaaaaccaa tatcaccagt atcaagccaa ccacctgatt ttaaagcttt
    18121 atcggtttct tcttgattat tgaagtatcc tgtcataatg ctaccgcctt tgacaagtac
    18181 actaccaacc atcatttgtg gtaactcttg accattctca tctacaatct ttactgtatg
    18241 ctctggcaga ggccgaccac aattaacgaa ttcattaaac ttcctacctt cagcttgcac
    18301 tcgcaccgcc attctttttt ccacgagcgt tttggtatcg accttcaagc tctcaatacc
    18361 acgattaatc ttggtaaacg atacagcaag tgttgattca gccaaaccat agcaaggtaa
    18421 aaaagcacga tcactaaatc cagctgaagc aaatttatct gcgaaattcc tcaaaatttc
    18481 tggacgaatc atttctgctc caatgcccgc agcacgccag caactgaggt ctaattcttc
    18541 caaatcagaa tctcgaacac gaagagcaca aagtttaagg ccaaaaggtt ggctaaatgc
    18601 aattgtgcag cgattacgac ttatcaactt taaccactgt aaaggtctaa ttgcaaaatc
    18661 tcgtgtagcc aaataatcga ccgtaagttg agcagccata ggagacaaca caagtccaac
    18721 taagcccatg tcatgataaa acggtaacca tgaagcgcag cgatcgcctg gattaacatc
    18781 taaaccatta cgaacgatac cacgtagatt acacatcaaa gcttcttccg taattacaac
    18841 accccgtggc atacgggtac taccagaagt aaattgcaaa tatgctgttt cgctcggtgt
    18901 attagattta agtgccttat ctgaattagg aagcgagtcc aactgagctg atgttccaac
    18961 ccattttaga tggccattgc ttaatccatc acctgcttct ttaagaaagc ttagataatc
    19021 aagattcgca attgcagcac ttgcaccact actctcaagc attccctgta attggcgaac
    19081 atagatcgaa tgactaccca gattaacagg gataggcatt gcaaacggaa ctaaacccgc
    19141 atagcggcaa gcaaaaaaca actcgataaa ctcgggcgat gtatcagcaa taagcgcaac
    19201 ccggctacca tgtgagaaac ccagtgacag caatctcctt gccttagatt gcgcattcac
    19261 tttaagtgta cgataaggaa gtactgagcg cagccctccc ttcgcatcat aaaaattata
    19321 gcccgtaaca cccttcgcgg catactctaa agcatctgtt aatgtctcaa aatcagccaa
    19381 tctttgactt aattcattaa gcgttggcgt aggtatcatt tttacagata cttcgtctga
    19441 aggatgaaaa tcagattctt gcattgttgg atcacccagc ttgattattg tcgaactcaa
    19501 gatcttcccc tagaagtaat agtgctggtc atcttatgtt tataagcacg acagatactg
    19561 acgctaaccc aacctggata tggtttaaat atactgttat ttcgttgcta aataaacaca
    19621 agtgtctcat attcgttgac gttggagctt atataagggt gattttcctg tcaagacgat
    19681 attatgtaaa tgttgtttaa tagcaacagc tcgattcgga aattttattt atttgataca
    19741 gttcacaaac tatttattaa gaatctgaaa agcagtaaat aaacaatgaa accaataatc
    19801 tactaccaac tctcaacaaa tgtttttcgc tgaatcggat caagtcgtgc tatttctttg
    19861 ttttcatagt aggaatctcc aacaatgcta tgcgtcgaaa tctcagccac aatcgcccca
    19921 gatttcgtca tgaaatcatg caatattcct ctatgtatca acagcctatc gccaggcttc
    19981 atgctaacct cacccgaatc tcgaagattg acggttatat cgccccataa taaataaaaa
    20041 gtttcttctt tctgttgatg caagtgcgga ggatgtttct gacccggcaa catgataagt
    20101 agcttataac agtattctcg atttatcaga ttgatcaaaa cacatcccat ttctcgaaat
    20161 tttgacaaac cataatgatg ggaaatttca atttgataat ccttccctaa ttcaatccca
    20221 gcttcataaa gcaaaccttt tgtatcatgt acaatctccc ttaccattcg aatggagtct
    20281 tgttcggttt tctcatagag agccgctcct gggacatagt cctgtgtggc aataaatttt
    20341 gcgcgaagct gcccaaattc cccgctagtc aactgaccct cagcacatgg catcgcaaaa
    20401 aaaacgtcat catcaagaat cggtgtacct tgttttacct gttgctttac atagacccct
    20461 cgctgaagct cgcgcaacga aactatttcg tcttcaccga taaacttatg atcatcacct
    20521 ccacagattt ctctcatctc ttctgcagcc gatacccaat gatcagcctg atcggggtct
    20581 aaagaatagc gattcaattt aataccctcc gctgacacgc caatatggcg ctcaagtaat
    20641 tgagcgccct ttgccaccgc tgccattacc cccattctat tttcgggtga ctcatgtcct
    20701 gaataaccta ctttcacgta aggataacga cgaatcattt ttcctaaaaa attcatgtga
    20761 atacgatcat tcggtgtcgg gtaaattgaa acacaatgca tcaatgcaaa cgctggcact
    20821 cgtttataaa aaaaactgac taaatgatca atatcagaga taccgagccc cccggtcgag
    20881 gcaataattg gcttttttac ttctgccatt gctgtcatca gaggccaatc ggtagctgaa
    20941 caacttgcca attttaagat ttggattcct aatttttgac aattagctac agatgactca
    21001 tcgaagggag ttgcaactga aataagatga tgatggtgga tagcctctac gagttcctcg
    21061 aaatcctgaa aatttagtcg cgcactctca aaacgtttaa tatgcttgat gtcctctcgc
    21121 tgcttgtaat caggatgaat gaaggtttct aattcgcgta attgtaattt aactgccgct
    21181 ttaatttggt gcttcttggc taaattagca acgacatcga taatttttaa tccatgcgct
    21241 acagaaccct catggttatt agccatttca aaaatataca gccgatcaaa tagttcaccc
    21301 atactcagta tcaaatcaca tttattactt atttattact ttttagttac gctactgact
    21361 tagataaagc ggctcgtttc atccaataaa atcattcgat catcgatata aaaatcagca
    21421 gccggcttac caaatttcaa atcatgatga agcacttgcc aatctgttaa ctgttgtcgg
    21481 gttatttcag accagtcaat accggtcatt gagcctcgcg ctgtaaataa tacaattcga
    21541 tgtcccgccc gatataaggc attaatcatt tcaatatttt tgctgattgg ctgacaaagc
    21601 gcatagtcat tattcgcaat gagtgaagca ataacgccat caatgtcaat tacaaacact
    21661 ttggaaccaa gagaatgtgt tttgttaata accgcattat taagattaag atcatgctgg
    21721 ttttccctca tcgcaaggta tttttccaca attgcaaaat cttcttccgt atcaatactt
    21781 agcatgaatt cagggataat cactgcaaag ctatgggccc ccagtagtga agcttggttg
    21841 agcaaacaat cgcgtgtaag ggcgtaggct gcgccattgc gataataggt ttgatgaagc
    21901 tgctgtcgcg caacgatatg agcaccttcc ttatcccata gatttaatgc tcccttatca
    21961 tctaagctta gctgttttag cggatgatat tttaaatcag taggacttac agtccaaact
    22021 gcatcaaaat tccctgcaat cagcttctct accgttgcaa caacatgctc cgctcgcctt
    22081 agcggtgatg tgggttgcaa catgacgata atatcatact gagtctcgtc catcgcctcc
    22141 gtagcatgga gcgcatgctg gagcacaggg atatctccca cttggtctcc cgccagttct
    22201 actggacgcc agaatggcac agccagtcca gccccctctg cgacctttgc gatttcagga
    22261 tgatcggttg aaaccacggc acgatcaatc caatctaatt tctgaatcaa taaaccaact
    22321 cgttcgacca aagaaatgcc gcctattttg cgcaaattct ttagtggcac gcctttactt
    22381 ccaccacgag caggaacaac aactagaatt ttttgatgag atagcacaat agaatcccga
    22441 gttcaactca tgttaacgat tcgagtccat aggggcgcgt ataatcaatc ttgatcacac
    22501 gcctttcttg atgggatttg atagccgctg caataacaag catcgtctct aaaccgtact
    22561 ccagagagat tggagatgaa agcgctggat tcgataagat acgttcaata tggttgattt
    22621 ctggtaaaaa atcatcgggt cgcgttttct caaaatactg ggcgcgctct acgtcatcct
    22681 gtgaccacaa tttaacagca tcagcgcctg gctttatatt aataaaccaa tctaaatatc
    22741 ctcctgaacc cagaatcctt aaatattttt ttgctggctc tgtgatcacg tcttgcacta
    22801 acaatccggt caatccggtc tcagtccgca aacttaactg agccaaacga tcataatacg
    22861 ctcccgcttc gttcaccata tcaagcatcg cagacacttc aatgatacga ccattcccgc
    22921 tcaagcgagc aaagtgctgc caaatggcta aaccatgaga atgttctgct agtgcacctc
    22981 ctccccgttg ggtataaccg agataggttt cctcgggtcc agaaatccag ggatgcgctt
    23041 taaagatacc ctgccaatgc tcacgaatct gcacatcaat cgtcaaaatt tttccaagct
    23101 tcccctcttg aagccattcc tctgcgagca acgtattact agtcaaagta tggttgtaac
    23161 caaccaagac acgggttcca acccgtttag ctataggaat taattgttca caactttgta
    23221 gaaggggcgt acataagggt ttttcaatca acaatacttg tggtggtgtt tttgcaagac
    23281 accccagcgc aagatcaatg tggctatcgg gaggagttcc gataataatc acttcaaaag
    23341 cttcgtccgc tacctcagcg ggttgcacta gacgaattgt ttcatcccat tttccatatc
    23401 gaccaggaaa aattttatcg cgtgtacgcc taagcgctgc aggatcaata tcacagatcg
    23461 taacgttcca acctaactgg cggcaagcat aggcaaggtg attaccaatc gaacctgcgc
    23521 ctaatatttt cactttaact tccatatttc agagcacact ctaattacct gtattcttaa
    23581 tgcctagctt caatctgaaa tacattctca gcaatattat tcacatgcag gtgctggaac
    23641 cgtcctaata atgaactcac ttgattgact ttattctcaa cttcttgcaa agaggtcatt
    23701 ggaaataaat gcctcaaata agtcgtattg ttacgatgat tcggtataac tgcttcaatt
    23761 cgcttcttga taaatgaacg ccaataggca gtgccaaggc ccgccattct tgaaaacaaa
    23821 ggaactttac ggcgtggttt tttgcataat tcttggagaa tttgggcgat ccgttcactt
    23881 gcaaatgtac gctcaaaaga agctagatgc tgctttgcta agtcatgata ttgcattcgg
    23941 ttaatggaat tcatcgggga atttccttga agcgaatttg ctactttttc tactaattgc
    24001 tgaaaattgt cacattgcac actcagtccg ttcggtagtg catgatcaaa tacctctgat
    24061 tttacggggc aataagcaag tgctatctta tcaagtaaaa aagactccac cgctgtcgta
    24121 caactattat ggataatcgt ttctgcagcc attatccaag gaacaacatt gccttcatag
    24181 ataattttca cattctgttt atgatttgct gcttcatacc aagcctgatg attctcagat
    24241 ggatgaggtc gcacgataat gtttatttca ggaaagtgat ctgccaactg tggaagcact
    24301 tcgataaaat gatcaaagag ttgcttttta tgccttgcaa gccctatttt caaatcattt
    24361 cgaacgagat ttgtattttc atctcttctt aactgattaa gtaaatggct ctcgtttgca
    24421 taataatgat tcacgcgcga gaaattggta ttaataagta taaatcgacc atagcgccgg
    24481 attaattgct ctacttcttg agtaaataac cctctcaatt caggacgcag tagatcagtc
    24541 cgtggatttc ctgtcagata tatcggaata ttagaaaacc caggatgctc acgtatagcc
    24601 gatgcatttg cttcgcccca tgcaaacaaa ccgacaagtt ctttcagtgt ccgagaatcc
    24661 agctttgtca atcggtacaa ctctggcgta gcccacacta gcccttcctc gtcccatgcg
    24721 acaacggtat agcctaattg cctcaacata cgcaaaactg tagcgctttt cgcagtcaag
    24781 ctttttccga tataaatacc tctgggtaac gatagcgcca acccatctat aactttcttt
    24841 gcacccacaa tagcctttat ccccttctca gcaataaaac aggccaatag tagttttgca
    24901 tcaaactctc gtgatttagt ctcacacggt aaaataacag tctctgcaat atttagactc
    24961 atacacgaaa acgtaacgat aaactttttg gaaaaaagat ttagattcta atcaaaaaaa
    25021 aatctcatcg tgtattggca atactgggtg gggctttggc aagatcgttg aaacttattc
    25081 tttcatatca attgcaagcg ttgagctctg ataagtacaa acaggccgat gcacttctaa
    25141 acattgagag gtgacgatta tctgatagct accgggaagt aaacgattgg tttcaaactc
    25201 aaaactaaat ttaccctgtt tgtcgctgat accaaacact tcaatgactt ctgttccatc
    25261 tgcacgaagc aatttagcat ttaacaaaac atcaggtgta gactgatcct gataattaac
    25321 ccacccattt actacaattt tttccccttg ggaatattct gttctagctt ccgataaaat
    25381 cggcctagag atatgagttg cattcacttc agaaaacgaa ccgctgatta ataaaaccag
    25441 aataattact acaaaacttt tacttttaat atcaaccatc atgctaaaca tggcttttct
    25501 tattttcctt tactataaat attaaactat tccgcatact ttcttttttt cttactttct
    25561 taactatagc ttaatcgaaa attagttgtc gatttttttt acatatgaac attaacttac
    25621 ggatcgtatg cgctaacaat tactcagcaa aaccattcta aattcaaaca aaattaatat
    25681 tttatgcttt cttccttgct tatcttatcg atttatatat tgtcgatatt tcttacatac
    25741 gatttttctt atgcactgac aatgacttga tcctaacaga aataatagct ttcagcgtta
    25801 tggcatgagg tttgcttatg aatatacgtg cttaggaata aatgaatcca acaacgcaaa
    25861 tttatgcgaa ataagatgaa aataataata gttcctagaa aataatggat caaattgaaa
    25921 gctgagcact tacaaaagat cacaaagcat cttttgtata aacactggaa tgcaccacgg
    25981 ttaggtccgt ggtgtttttt ttagtaatag taaatgtcca aaaatcgcta ttgacgcttg
    26041 tcaacatagc aataataagt gacgcatcta atttgtagtt ctatgaaact aattatcgaa
    26101 gactgatgat tggccaatca ttttgtcgtg cgtgattctc aagtgttgca tcgggatcaa
    26161 ccgcaactgg atgtgttact ttccttaaga gtggtaaatc attcaaagaa tcactataaa
    26221 accaactttc aagaaaggat aaccaagtaa gattgtgtgc atcaagccat tgctctaacc
    26281 tcgtaatctt tccttcccta aaacaaggca taccagaaac ccggcctgta aattgaccct
    26341 ctttttgctc tggttctgtt gcaattaaat gatctacacc cagtattctt gctatgggcg
    26401 aagtcacaaa gctattcgtt gctgtaatga tgatacaaag attccgttca agcttatgtt
    26461 ttgcgatcaa ctcaagcgca cccggcgcaa tgatcggcct aatttttttt tggataaatt
    26521 cctcgcgcca agtgttcaat tgttctctcg gatgtcgtgc aaggggttta agttgaaaat
    26581 ctaaaaattc gtgaatatcg agagtaccca ccttgtattg ctcatagaat tcaatattgc
    26641 gcgcttcata aacttcacga tccaatactt tctgttcgat taaaaactgg gcccactgga
    26701 aatcgctatc gccagctagt aatgtattat caagatcaaa caaagctagt ttcataacac
    26761 ctcatttaaa aattgtcgta atagcggaag tgtaatcgga cgctgcagca tcagtgaata
    26821 tcgacccagg gcatcgatca ttttgatcag atacggcata tctcgtttct cgtagtgcaa
    26881 caaataatca caaacctctg caggcaattc gaatccatta ttaaccgcat gattcttcat
    26941 cgccttacct ttctcatcat ctgttagctc atgaatctgg taaaccaatc cccagctcaa
    27001 gcgggttatt acatcttgcc ggagctgaag ttgggttgga actgttttac cactcaccaa
    27061 aaacagcgcc aggcaatcat cacgtgcctg attatacaaa ttaaacagtt ttatttgcgc
    27121 ttctgggcct aaccgatcaa catcatcaac gataatacaa cccggtaaat cgtcactgag
    27181 ttcaaattca gtatattgat cacatgcgaa ataagccact tgatagcgtt ttaatcttat
    27241 acaagcatca caaactgatt tgagcaaatg acttttacca gatccacttc caccccagca
    27301 atagataaaa ttaccagaac tcgatgtggc tagaaactca ttcaaggcat gaagcaactc
    27361 aacattacgt cctggaataa aattttctaa cgttggaata ttttctaggg aaagatcaag
    27421 caacaattgc ttcattgagc gattccctga tcagttcttc cgtagcgaca ttaaatacag
    27481 taaatgctat gaaaacaaaa tagcgatcaa aataactgat ttcttactta ctttttttgt
    27541 gctgcagcgc atattaaaca gagaattttt gctaaaactt ggaaattaac gtttccaata
    27601 tactctcaac ctattctctt ctgggttata gcgaaattcc agatccccct gataagcatg
    27661 atgaatcgct tctcccattc ctcgcacaag atgaatatca gttgtcgtta caagggttaa
    27721 gtcaacgctc tcctcaattt tcatgatgcg tttaagtgga tgctctgtac actggtgttt
    27781 ctcgtgattt ctcactaaat gcataatttc ttctcgatgc tctaaataaa acactccctg
    27841 caaagtcaaa taaccggcgg ggtaatcatc ccgaatacga tgacatgctg ggcaaattcc
    27901 ttgatgtgca ctagctgggg ctgttaacca ctgccaccga ccttgatggt ataccgcctg
    27961 acactgtgaa caaactgttg gttcagctaa tttgcttttt aattgatagg tatcgtgtat
    28021 ttcttcttta aatatgccat catggcgagg aatggcacgg tatccatcta gaatcttcga
    28081 gtgactcatc ttagctctcc tatcttaata attgacattc catcttttct tacataaaac
    28141 cttgctctat tttaaacaga ctaagagcaa caagcacaat tcattgacct taagctatat
    28201 ttgtatatag gggcatataa gaaaaaatca attactttta ttctccccag cgcgggatca
    28261 gggaatgctc aacaccaaga tgatcgagta ttctagcgac tacaaaatca acgatgtctt
    28321 ggatattttt ggggtgatgg taaaaaccag gattggcagg caaaatgatg acaccacacc
    28381 gcgcaagtct aagcatattc tctagatgaa tcgctgaaaa tggcatttca cggggtacta
    28441 aaattagttt gcgattttct ttaatcataa cgtctgctgc acgctcaatt aacttttgat
    28501 tcaatcccga agcgatagca gaaagcgtgc ccatcgtaca aggacaaaca accatcgctt
    28561 ctgctggatt cgaacctgat gcaacgggcg caaaccactc ttcacggcca aataccttga
    28621 gcagatcttt ggatacgtta aagtagtcaa tgaaaaaatg ctctgcatct tgagaacgag
    28681 agggtagcgc cagctgcatt tcctgttgcg caacaatttg ggctacttgt gaatacaaca
    28741 aataaacccg tatatgctgc gctaacaatt gctccagcaa tcggataccg taaggcattc
    28801 ccgatgctcc tgtgaaagca agtgtaattg tgcgtggtgg tgccttatct atcacagatc
    28861 ccctgccatc tcctgctcat ggcgcttaat aaattcctgc gtattgtcaa tccctctgag
    28921 ctgtagaata tagttacgga ccgcagcttc tactaatact gctaaattac ggccagccgc
    28981 aacagggatg gttactttcc gaattttcac attcaaaatt tcttcatcaa ggttactcaa
    29041 gggtaaacgt tcaaaagaac cgatatcagc agcactcgtt ttttctagat gaacaattag
    29101 cttcatattc ttccgccgac gaacggctgt ctcaccaaag atggtacgaa tatttagcat
    29161 gccgagcccc ctaacttcaa gataatcacg aagcaacggg gggcaacacc cttccaatgt
    29221 ttctggaccg atacgatgga tttctacaac atcatctgca actaaaccat ggccacgact
    29281 tatcaattct aaagcaagtt cacttttccc aaccccgcta tcaccggtaa taagcacacc
    29341 cattcccagc acgtcgagca atacaccatg cttacttgtg cttggtgcta gaacttttgc
    29401 taaataagat cgtagtaccc agataacatc caaactggat gaagatgagc agaataaagg
    29461 aatcttggtc ttgatcgcaa atgattgtaa taattcaggt acggtcgccc catcagctac
    29521 aaccaaacaa gccaaactac tctgcgctag tttatcaagt ttttgttgaa aagaggcttt
    29581 atcgagctga ttcagataat caatggtaag gttactgagt atctgaatcc aattagggtg
    29641 aataaaattg agatggccga ttaatccttg actagaacat gaggtatcat cattactcaa
    29701 atggttgcta ccatactcca gtcccaaatt ccaaaccaag ccaagctttt ctttattatc
    29761 ttcaaataat tgcgtgatac taatttggga cattatcagg tatccaatca gtaatcagct
    29821 gatgaatttc cttagtattt ttactcataa gtaatttttc acgaaagctt ttatcactaa
    29881 acatttgcgc caattcactt aaaatttgta aatgcttatc tgtcgctttt tcaggtacaa
    29941 gtagtataca agcgatattt accggcaatc catcgggcgc atcaaacgga atcgcttctt
    30001 tcattctcac caatgcaacc gttgcttccc gcaacccttt aattctacca tgtggaatag
    30061 caacgccctg acccaggccc gttgatccaa gcttctctcg ggcaaataag ctatcaaaca
    30121 cctgactccg agccacatgc gaagtattct caaataataa ccctgcttgc tcaaatacac
    30181 gtttcttgct agcaacgtcg agatcaataa ttacattcga ttcaggtaaa aactgagaaa
    30241 caagattcat aagaaaaata aacagttact ttaaacaact actacttact gatcagtgta
    30301 agatcaatct gatcgatgaa atcattaaaa aaattgattg gtaattacgc ttctggttct
    30361 tgatcaattt cttttttctt gatcgtgtcg tgattacgac gctccatatt tttttcctta
    30421 tatttaagga tttgtcgatc taacttatca accaggctat ctattgaggc atacatatca
    30481 gcgccatccg cttcaacgaa aatatcttta ccccggacat ggacattggc ttccgctttc
    30541 tgtttatgtt tttctactga caaaataacg cttacatcga ttacatgatc aaaatgtcgc
    30601 gtaattttac ttatcttcga aacaacgtac tcacgcatag ccggcgtaac ttctacgtga
    30661 ttgcctgtaa gatttaaatt catcatttcc cccttcgatt aagtaatttc attgttctca
    30721 gctaaattga caggccccca tgaacatcct tgccttgtct tttctattca aatttcaaga
    30781 aaaaaacggt aaatgaattc ataatggata tcgaattgag tctattcgct atattaatta
    30841 ttttattatt ggccttaagt aaggatttat agaaataact caagatagtc aacagaactt
    30901 tgggatatca gatctataac aaaaactgat cattagaaaa caaatccaac caatttattt
    30961 cagattaacg gaaaatcgaa tggttaattg tattatttgt aacgcttcaa ttttttaact
    31021 caaatcaata cacactaatt tttttcaatt agcgattata caatacacac taaccttgat
    31081 acgaccccta aaaaggaatt ggtttaaaaa atgatttctt tgaattgtgc attaggtgtt
    31141 attacctgca gtttttgcaa ctgaatgagt tcaatttgct gtaatcctgt tgcattctca
    31201 acgatcaaaa actctcgctt ttcagcagta gtgatgatat ttttcgcttt accttggatt
    31261 atttgttggt cttttaatgt caactgcacc tcataaccat acaagcaagc cacttccagg
    31321 taatcatgca gttcacagtc aatgatggat tgagtcatct tttctgattt ttccttttct
    31381 ttatcaatga acagtaattt tactgctaac attctagaat tgtagaaaat tagttaagca
    31441 gtctctaaca ttgaattttt tgattcatct gctacagtac aaattattat caaggagcaa
    31501 tttatgaaaa aaatcatcaa atttgtaccg cttgcaacca ttatcatgct gagcagttgc
    31561 gcaacgatat cagatactca acggggaaca gcccagggtg cggcaattgg tgcaggtgct
    31621 ggtgcagcca tcggggcaat ttttggtggc ggtaaaggtg ctgcaatcgg cgcgggatcc
    31681 ggcgctgtac tcggtgctgg tgccggctat atttggtctc aacgcatgga agaacaaaag
    31741 aaacaaatgg agtctgcaac ggctggcacg ggcgtgcaag tttcacaaac tacggataat
    31801 cgtttaaagt tgaatatacc cagtgatatt tctttcgata ctggacgtga tgatattaag
    31861 tccaactttc gtcctatatt ggataacttc gccacatcgt tactcaataa tcccggaact
    31921 tctgtaatga tcgttggaca tactgataat actggcacag atgccatcaa taacccatta
    31981 tcggtcaatc gtgctgcaag tacgcgcgac tacctggtta gcaaaggagt accgattaat
    32041 cgcattcaaa ttgatggccg tggctccggt gaacccgttg ctgccaatga taccccagcg
    32101 aatcgtgcag aaaatagacg cgttgagatt tttgtttcag aaatacaaga agaagcgccc
    32161 cctgtttcta aaccctagtc ggtataatta acacaaaaaa gcagggttgc tcgttaatag
    32221 caacgctgtt tcattgggag ataatctatc cccgatcagt agcatcattt actcaaaatt
    32281 tattctataa gacgctatga cagagttacc aaatcaaaaa gaaggttggg aaaaagaatt
    32341 actagaaaaa gttgtgttat caacgctacg cgaacaacgt cggacgcgac tttggagcat
    32401 cttttttaga tctttagcct tcgcttatct tttcatatta ttgttttggg gaatggggct
    32461 atttagcgat gattccacca gctcgtcgga tcgtcacact gcattaatcg atgtgcgcgg
    32521 agaaatcatg gctgacagcc tcagtagcgc agataacgtc aatgcaggtc tccaaaaagc
    32581 gtttaaagat aagaatacgg ctggtgtgat tattcgcatc aacagcccag gaggaagtcc
    32641 agtacaagct ggtaatatca atgatgaaat ccgcagactc cgcactaaac atcctcaaat
    32701 cccagtttat gcagtcgtcg aagatatttg cgcttccggt ggatattata ttgcggtcgc
    32761 tgctgacaaa atctttgtgg ataaggcaag tatcgtagga tcgattggtg ttgtgttaga
    32821 tagctttgga tttactggta ccttagaaaa attaggtgtt gagcgaagat tattgactgc
    32881 gggagagaat aaaagctttc ttgatccgtt ctcaccatct gaccccaaac aaagagagca
    32941 tgcaagaaaa atgcttggcg aagttcatga gcagttcatt cgtatcgttc aacatggcag
    33001 aggtgatcga ttaaaagatt cacccgaaat ttttagtggg atggtttgga caggtcaaaa
    33061 aagcattgat ctcggcttaa ctgatgagtt aggtaacaca gaatatgtgg ctcgcgaaat
    33121 catcaaagca gaacgtatta tcgattacac cacaaaagaa ggtctggctg aacattttgc
    33181 caaacggttc gggaaagttg caacgggcct atttatcaat acattcagta gtttggaatt
    33241 acgctgagta tttttatgac tggcaaagtt taccgctcta ggatttcggt aagagccctc
    33301 gatactccaa tttgccattg aggtacatta aggcggaacg ttttctgaaa tttatccgta
    33361 cttaatcgag aattacacgg tcgctttgct ggcgtcttaa aattacttgc cggtacagga
    33421 acaatagcat tagtcttcaa cttcaaatct atgccctgtg cttgcgcaaa attaataata
    33481 aaactggcat agccatgcca tgagatttca ccttccgcaa caagatggta gagtcccgat
    33541 aactcaggtc gattgatcgc catcataatt gcatgcgctg ttgtatctgc caaataatca
    33601 gcccccgttg gtgcaccaat ttggtcatta atcaccgtca gttgatcccg ttcattcgcg
    33661 agtcgtaata tcgatttaat gaaattattg ccccgtaccg cataaaccca gctcgttcga
    33721 aaaattagat ataaacagcc ggtttctcga attgcttctt caccctctag ctttgtctga
    33781 ccatagacat tgagtggtgc aggattatcc gtttctaacc aaggtttgtt tccacttcca
    33841 ttgaatacat aatccgtaga ataatgaacc agccaagcgc cagcacgttt tgcctcatgt
    33901 gccagaacag cagtcgcttc agtattgatt tgccgtgcta aaactggctc gctttccgct
    33961 ttatcaaccg cagtataagc aacagcatta acaatcacat ccggtgcgat tgccctaatt
    34021 atctgagtaa tgcccgctgt atccgtgata tcaccacaat attcttggct gaatcgatcc
    34081 aatgcgatta ccctacctaa gggcgctaaa ctacgctgca attcccaacc tacttgccca
    34141 ttctttccaa ataacaggat tttcatttaa tttactgttg gtaatgtgta gttatccatt
    34201 ttcgatattc accggattgt acgttggcta cccaatcttt atgggctaaa taccattgca
    34261 ccgttttacg aattccagtt tcaaatgtct ctaggggcct ccatgccagt tcgctctcaa
    34321 tcttctgtgc atcgatcgcg tagcgccgat catgacccgg tcggtcggta acatatgtta
    34381 tcaatcgatg ataatctcca gggcaaaact cattcagtaa atcacagatt aaacgaataa
    34441 tttcaatatt tggcttttca ttccaccccc cgatattgta ggtttctcct ggaacccctg
    34501 cctccaagac acgtgaaata gcactacaat gatctttcac ataaagccaa tctcgaatct
    34561 gcaaaccgtc accatagact ggcaatgact tcccggctaa agcattcaca atcacgagag
    34621 gaatcagctt ctcaggaaat tgaaagggcc catagttatt tgagcaattc gtggtcagtg
    34681 tcggaaatcc ataagtatgg tgataagccc gaactagatg atcgcttgaa gccttgcttg
    34741 cagaataagg actattgggg ctataaggat gcttctccgt aaaagcaggt acatccggtg
    34801 cgagtgaacc ataaacctca tcagtcgaaa catgtagaaa acgaaaatcg taatgcgcct
    34861 ttcctgttag cttattccag taatggcgag ctgcctctag caaacgaaaa gtaccgacga
    34921 tattggtttg aataaaatca tcaggtccat ggatagaacg atcgacatgg ctctccgctg
    34981 caaaatggat caccgctctg ggttgatagt tatcaaacag ttgattcaat ttctgtccat
    35041 cacagatatc gccttgtaca aaaatatgac gagcatcact ctgtaaggaa ataaggtttt
    35101 gcaaattacc tgcataagtc aacttatcta aattaacaac gggctcatca atacggctta
    35161 gccaatccag aataaaattt gctccaataa agcctgcacc acctgtaacc agaatcataa
    35221 atttctcttt acctctaaaa tttttaactg ggatgccatg agggcattag cccttgctca
    35281 cctggcataa cctgaaaccc acaatcaata ccaattcctg caacccagac aagctgatcg
    35341 ccacttagca acaatggtaa atcatttctc tcccattctg gtattgccgc ttcctggaaa
    35401 attttcttca aactccgtcg aggacgccta caatttggtt gaaatcgttc accccctttt
    35461 ctcagacgca cagcaaccgg attttccatt aactttttag catttatccc agttcccgta
    35521 ctatttgacc atttaatcac accacccaat ggttcgatta gccagttcag ttctccatgc
    35581 cagtaaaaga gttgatcttg atcgaaatca ggcggtttgg cttttggtag cagtttgact
    35641 aaatttttat acaagcgaat ctcgaattcc ccaaatacca aatgaagatg attgtctgat
    35701 ttaactgccc acaactgccg tagaatttct tctaattttt gagtgctcgg ctgaatgata
    35761 tcgtgtcgtg aaaaaatata acgcagtaaa tttttagctc gcagaaagtc tagcttgcgt
    35821 aatcgttcaa tgtggatatc tcccatcacc atgactgatt ggctgtcatt ttccgctaaa
    35881 ccatccaata attgaatggc ttcaccaata tgtcgacttg ttcgcgctaa tgtttctcga
    35941 taagcaggat atcgttgctc taatacagga aaaatctggt ggcgcagaaa attccgatga
    36001 taggtcgtat cgtcattgct ttcgtcggtt acccacatta atttattttg taaggcgtag
    36061 tcttgaataa tatcgcgcga aacagttaag aggggtctca atagttttac attattcgcc
    36121 gctgaaccaa gtgacctcat aataggcatt ccagccaatc ctctcagtcc tgcgccacgt
    36181 aacaattgca attggagtgt ctcagcctga tcatctaaat gatgcgctag tacaataaaa
    36241 tccgctgata tcgctttaaa tatagcatag cgaccgcgtc gcgccgctgc ttccaaactt
    36301 acttgagatt gtttcttgat actcagatga aaaacggtca gtggaacccc aagcgcatca
    36361 caataacttt gacagaaatc gctccactgc tttgcattag gactaatgcc atgttcaaca
    36421 tgaatcgcag aaagattaaa agtaaattgt tttgaaatct gagcaagaat ttgcagtaaa
    36481 accactgaat caacgccacc actcaaagca agcgctaggt gattttgctc ggacacatat
    36541 tgccgtaaaa cgacttcaac cgtatcacgt atgccgttca ggcttgactt ccttatactt
    36601 cccatgtccc attaaacgat taaaccgccg ttctaataag ctttcaatag atatatcttg
    36661 aagttttctt agggattctt gtaatacatt ttttatggat agcattgtgg ctggatagtc
    36721 acgatgggct cccccaatgg gttcattgat aatgccatcg attaatgaca tctctttcaa
    36781 tcgatcagcg gtaattccca taatctccgc agcttcagac gctttctcag cacttttcca
    36841 caaaatagag gcgcaacctt caggcgaaat aacagaataa gtcgcatatt gcatcatcaa
    36901 aaccatatca cccactgcta cagccagtgc accaccagat ccaccttcgc caatgatgac
    36961 agaaacgatc ggcacttcta accctgccat tacatagaga ttctttccaa tggcttctga
    37021 ttgcccgcgc tcttcagcat caatcccggg ataagcgcct ggcgtatcga taaacgtaat
    37081 caaagggatt gaaaattttt cagctaacga catcaaccgc agcgccttac gatacccttc
    37141 tggttttggc ataccaaaat ttcgataaat cttctctttc gtatcacgcc ctttctgatg
    37201 accgatcacc atgatcgatt gaccattaaa ccgtgccagc ccacccacga ttgcatgatc
    37261 gtctgcaaaa ttacgatccc catgcagctc ttcaaaatcc gtgaaaagat gctgaatata
    37321 atccagcgca taagggcgct gtggatgacg agcgacttgt gaaatctgcc acggtgttaa
    37381 ttttgcatag acacttttag tcagtgcctg gctttttgct tgtaatttag caatttctgc
    37441 agaaatatcc aaagctgaat tatcttgagc aaaccgtaat tgctcaattt tctcttcaaa
    37501 ttcttcaatt cctttctcaa aatctaaaaa cgttgtcttc atgaaaacta aaatttattc
    37561 catcttaaag taggaaatga tacagcattt accaagaaca ctttaccagc aattgcatat
    37621 ggacacgcca gaacaagaat ttcaattttt gggtaaattg cctaaaattt taaaaaccat
    37681 catcgctatt tttgaaaccg gatgaatcca tgagtaatcc gttcacactt ttagttgtgc
    37741 agtaagtgat tcacttccgt tagatcaaaa tgctcggcgt cgaacggttc accgaaccat
    37801 tccagtatac cgccatgctc cgggtgacta gggtcagcta aggcttcaag gaacatttca
    37861 tacccaggcg ggccaccaac atcctccggt gggcaggcac gacttccttt aagacaggtt
    37921 ggtaatttag ttcccatttc aaagggtagg attttctcta gaacaatttc atgatcccag
    37981 ttatcaccga agtcgtaggt ataacggatt ttgtcttttt cctgtttcaa tatctggcta
    38041 atacgaaatt tggattcatc gtgtatcgct gaaggaaaat cttcatcggg taggccatag
    38101 cgttctccac cttttgcaaa ttcatgcaag tgcgaatcgc tccaacccat gacgatttgc
    38161 acgacaagat gtaaatttgc taggctgtca gtgctggcaa tcaaaaacct gcgccatatc
    38221 ggtggacgaa tataattcaa ggtaacttta agttgataaa tcgcgcgtaa agctgtcgcc
    38281 tgtgtgcgtc ctgtcattcg tagttttaac tccccaaaat caaagctgat tctcatctaa
    38341 attgttgcta acaaccgcaa tctattagat tcttaatatt ataattccat caagatgatg
    38401 gtctggtttc ttcgtagaaa tcgattcatt gttttcctgt agcgctaacc attttgcttc
    38461 ctaagcacct agtcttattg tcttttacaa aattcgacca aattaaaggt taaatcagga
    38521 attactgcta actggctaga ccaagttaaa gcatgttggt ggaaaattgc atggtgttgc
    38581 caaaaatgat tccaatcgag acgctcactt ccattccaac catgatggaa agcccggagt
    38641 gcctgggcag actctttggt caatgactgt gaatatcgat taagaaatgc ttcaagctta
    38701 acatagtgaa cattatcagc ttggggataa atttgccaaa caaagggacg tgccgcccat
    38761 tgagctcgta caaatgaatc ttcccctctg acaaaattgc aatcacatag ccacagtaat
    38821 tgatcatatt gcgcttgagg tagaaatgga atgatttgca cggtaagttt atcctgttga
    38881 ataacatctc ccgcttttaa tgctgatcgg tttaacccat ttctgagtag agtcaacgcc
    38941 tgaccttcgg ggataacaca gcgaatcgga gtctgcgaat gagcccaagc ttctagcaaa
    39001 ttagaaatcg gcgctgtgtc atagcaaaaa agagaaatga cgatctcggt ttcatcagga
    39061 ataggtaaat cattttgcaa ccaccaatta gctgcattgt gttgcacgct atcgcgttgc
    39121 actagtagat ctttctctcg cagtaatcca ccggtattcg ctgtaaatcc tggaaagaaa
    39181 aaatatttcg ttagaggtaa acggggatgc ggtgagcaaa gtccatgata ttcctccacc
    39241 catgattctg cagatagata ttccaagttg atccataccg gttgacggct tcgagaatga
    39301 ctcggaagca ttgcctggat atatttttcc ggcagctcgc acccataagc ttcaatcacc
    39361 acgtcagcag gaataatatc tgggaatgga tcagtccaat gattgatttg cacaccgcaa
    39421 cgctgttgaa acgtttgatg tggatcaaca ctcgggcaaa tgcgctgcag actcgctaaa
    39481 tcatccaccc acaatcgaac agctaagcca tattctgttg ttaactgccg tgataatcgc
    39541 caacaaaagc caatatcacc atagttatcg atgacacggc agaatagatc ccagctcact
    39601 acccagctaa tcgattgata gtatcattgt gccaattact tggttgcttt tgctttggtt
    39661 ttagataccg cagctttagg cgctggttta agaacaaagg ttattttcaa cacgacgcgg
    39721 tactcaacaa tttttccacc tgagatttct acagattgat ctttcaccca cgctgaggtg
    39781 atatcggtca tagtatctgc agcacgggca acccctttct taatcgcatc atcaaaactc
    39841 actttactcg atgaaatgat ttcaaaaact tttgcaattg acatatcaat cccctatgaa
    39901 ttgttattga gaaaaacctt cacaagctca ttttattacc aaatcaaagc taactgtatg
    39961 aatttcatac gcaacaacaa cccaaccaat taacctacaa ttctgactca accttactgc
    40021 aacgaaaggc tttccagtgc ttttgtatga tcccattttc catggaacaa atgccctttt
    40081 ttctcaggtg tacggtcgct tgtccaagta atcgctttcc catccggtgt aaaaacaggt
    40141 aagccatcga atccatcctt atccgttaca cgaaccggtt gtttttttcc gtcgacatca
    40201 acgatataaa gctcaaaatt acgatgacca tgaagattcg ttgagaaaat gatgtattgc
    40261 ccagagggat gataaaaagg cgcccacgac atcatgttta agtgcgtaat ttgtttctgg
    40321 tctgatccat cgatattcat tgtaaagatt tcagcttctc gaccattggt tgagaatcgc
    40381 cgccatacga ttctttttcc atcggggctg aaaaatggcc ctccatcata gacattcgtg
    40441 tgagtaagcc gtttcacatt ggagccatca gcattcatga tgtaaatatc gatgaaagca
    40501 gaaggatctt tttttaataa tactgcttcc tcatcggata atttttcagt ataagccctg
    40561 cgattcgaag caaatgcaat aagcttacca tccggtgacc aagaagcttc ggcatcatag
    40621 ccgagggtat tcgttagatt cttgattctg ccacccgtaa aatcggtttc gtaaatatca
    40681 taatattcat cgtaatccca ggcataagat tgcttctcac ctgatttcct ccgttcgatc
    40741 tcagcaagca tttttgcttt tgcatctgaa tcttcgtgcg tcgatgaaaa caaaactttc
    40801 tcttgcgatg ggtgaaccca tgcacaagtc gttttcccat cccctggaga gacttgctga
    40861 accttaccgc tgaccagatc ttttaaataa atctggtaaa acgggttact ttcagatact
    40921 tctgcttgat agaccatcca tttcatatcg gcccgatagt aggcttctcc aactctcacc
    40981 tctttatccg acaattgatg atcttccaca ataaaatcgg aagcagtaac actcattgcc
    41041 aagcaaagta acgctaaact cagaaatcca attgaccagc ttgatcgctt aaatgattga
    41101 acgtgttttt ccataggact gataaacctc acaaattaat ttgatccatt taaacatagc
    41161 ttgaaaaaga aagtaagcct gccagcttag tttcaaagct attcccaata accataacta
    41221 tctgccaaaa tgcacgacat gatcgaacaa gagaataaaa ccatgaaaac tgagactcgc
    41281 ctgatagtag ccatttgcct cacgatgatt tatagccttt tcgcaatgtc tgctagtgcg
    41341 agcgatgaga cctttcatca tcaaatgcaa atcaccctga tacctagcgc ttctgaaatt
    41401 caagtcacca gcacaataca ggtacctaag cacttattga aatcagatag cgaaacaaag
    41461 ctcgatttta gcttgcatgc tgacttatcc atcacagaaa tgaatggtgc taagctttct
    41521 ccacaacaaa agattaatac gctgctcgcg agacctgttc cagtcaaacg ttatactgtc
    41581 accctccccg cgcaacaaaa ctcatttcag ttatcgttcc aaggaaagat tcaccacacc
    41641 gtacaaggcc ctggtgaaga atatgcacgc agttttagct acacaccggg tctcatttct
    41701 gaagaaggtg tctttctagc aaattcaaca gcctggtatc cccaatttga aaacgcgatg
    41761 gtttcttttc agcttgaagt caaagtacca caagactggg atgctgtcag tcaaggtaca
    41821 ttgctaagtg agcaaaaatc aagcaccacg caacaagtgg tttgggaaga aaaacacccc
    41881 caagacgata tctacctaat cgcgggtcgc tttgaacgct atacccagcc agccactacg
    41941 accataactt cctatgttta tctacgtggc tccgataaag cattagctca aaaatatttg
    42001 gataccaccg cgcaatatat cgcgatgtat aacaagttac tcggacccta tccttacact
    42061 aaatttgcgc tggttgaaaa tttctgggag accgggtatg gaatgccatc ttttacttta
    42121 ttaggcccca aagtcgttcg ctttcctttt attttgcaca cgtcttaccc ccatgaaatt
    42181 cttcacaact actggggtaa cggggtattc gtcgattaca cgcaaggaaa ttggtctgaa
    42241 ggattaactg cttatcttgc agatcatcta gttgccgaac aaggcagaaa aggcgaggaa
    42301 tatcgccgcg atgtactgca aaaatacact gacttcgtta ataaagaaaa agatttcccc
    42361 atcaaacagt ttacttcgcg ccatagctct agctcagaag ctgtgggcta tggtaaaacc
    42421 atgatgtttt tccatatgct gcgtcaaaca ttaggggatg acgattttgt tcgtctattg
    42481 cgtgcttttt atcagcaata caaattcaaa caagcaacat ttcaggattt actagcaagt
    42541 atcaaccact atacccgcag tgactttacg ccattttttg cgcaatggat caaccaaagt
    42601 ggggcgccca atctacacct ccaagaagct caagccgaac cggctgctaa tggctttaga
    42661 ctcaaagtga cgcttaaaca aacacaacca gggaaaccct acgacttaac gctaccgatt
    42721 gctattcatc ttgaggggca accacaagcg tatcagcatt cgatcaaact taaccaaacc
    42781 gttcaaacca tctcgattga tttaccgcag cgccccgtcc gaatcgatat cgatccacaa
    42841 tttgatgtat tcagacgact cgatagccgt gaaattccat cagcactttc tcagggattt
    42901 ggtgcagaaa acgcattagt gatattacct tcaaaggctg atcctgcttt gctgcaggcc
    42961 tatcagcaat tagctgctaa ttggcagaaa acccaatcaa tacgactcga aatcatccaa
    43021 gatcatcaac tcgacaaact gccagtcgat cgcaccatat gggtattagg atgggagaat
    43081 cgatttagtc aacccgtgct gtctacgctc gaatcacaaa aggtttccta tcaagcgaat
    43141 acattacaaa ttgcccagca acactatcat caggctgagc acgcaatcgt attaactgca
    43201 aggcaaccag aaaatgcaga caaaacccta ctatgggttg ccgcacatca tcccagtgcg
    43261 attgcagaac tagcccgaaa attaccccac taccgtaaat acagtttctt ggtattcaaa
    43321 ggcgatgaat taacgaatat tgataaagga caatggccaa ttacgcaatc accgcttacg
    43381 caaaaaatcc accagcaaga tgatccttct ttcgcatctg atcatgtcgg ctcactatca
    43441 ccccgtcgca cccttgcaga attacctcca ctgttctctg aaacacgaat gctagctgat
    43501 atcacgcact tagcgagcgc aacctttaaa gggcgagagc ttggaacccc cgaattggat
    43561 ctagccgcag actatatcgc acagcagttt caccaggcag ggttgctacc cggtggtgac
    43621 aatcagcgtt ttttccaagt ttggcaacat gatgttggtc cacctaaagg tatcgtttcc
    43681 ttacgaaacg tggtgggaat attgccggga tctaacccac aactagataa agaaagcctt
    43741 gtgattgggg ctcactatga tcatcttggg atgggttggc ctgatgttcg gcacgacaac
    43801 caaggtaaaa ttcactatgg tgccgatgat aacgcgagcg gtattgccat aatgctggag
    43861 cttgccagac aaattgcccc aaaatggcaa ccgcagcgaa caatcatttt tattgcattt
    43921 actggagagg aggcgaattt gctgggctca acttattacg caaaccatga taacaactac
    43981 cctacaaaaa aaatcatcgc catgctcaac ctcgatacgg ttggaaggct tggtaacaat
    44041 ccggtaacga tctttggtac cggt
//
LOCUS       DAOQLJ010000003        37248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_329_length_37248_cov_6.452666, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000003 DAOQLJ010000000
VERSION     DAOQLJ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 37248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37248
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_329_length_37248_cov_6.452666"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3281
                     /locus_tag="PK522_00420"
     CDS             <1..3281
                     /locus_tag="PK522_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNC40226.1"
                     /translation="RLGLRVAALDSTAMSMDIRGDFIGKSIESLPHWQGRLYFELKQI
                     DLAVWHRWIPFPGEIDIKQGKGALRGWLTLTEGTINDWLIDADLHRINASLDTNLPKL
                     ELDFLQGRISVKQSNQSGSLVQEWTAHQLNFKLAHQQIVTNPINAIWRQHHDPKPSEA
                     SNYLQIDEIDVGVWANLLEYLPVDASVRKQVEKYSPHGKIEHMQLSWKGSWSEPHAYQ
                     LDTQFNDLSLNNIQMGHLISIHGLSGNIQGTEQEGRLNLNSQSVSLQLRQPFPQSIVF
                     DTLATRVGWKKIAEQESTTYKIEDLAFNNQHLAGSAIRGSYVHKQNKPDWIDIQGNLN
                     QADVNGLSRLLLPFTNKTQLAWIDESSLSGQLKNTQFNIQGNLSDSSANDFSWSFATE
                     MVNISASIGAQWPSITNISGHLTASSTLINAIVSEGNIETIQFKDTSIHIADLQSKKP
                     LLKIKGEATGKTKQIIQLIKESPLTQNTFDIDIPGVITGKGKLQFESTIPILSKEKIV
                     DIDLKGQYQFINNQIDLGPELPAISKVNGKVSFTHNTWDFDEIHAQLLGGPLVIRSKP
                     LNNDKKQIALEGQANFDRFNTRLSQKSPSALQFWLQHIHGTTDWTAIIDMNNSQITAL
                     QIASSLNGVTSVLPAPFSKTHDEIIPLTFTKTAIDSANEKLFCQLGEKVTTEIHRTQT
                     KQGDFIPVRGVMSFGNRSATLPSEKLTLMHGPIPLLEWDKWQEMLDQHEAIVGLSKQQ
                     SRGIDALLTHHIRFDLNVGVLDFLGSRFNDFILHADKQGSEWTTDVTSREVTGKIYWN
                     ATGKKKATARLKKLVVPESLPENGSAERKKHESQNWPIIDLHADEFFYKEKLLGQLTL
                     LAEQNKENWVVDQMEISHKHSIFTAKGIWKNRQEPFSMYAEIKLTTSDIGKFLNRVGY
                     PDRIARGEGSLEGHLSWQGKPFSVDFPTLSGQLKLAAKHGQFTKFKPGMSKLLGIFDL
                     KALPRRLTLDFYDIFSQGFGFDDILGNVRIDHGIATFVDLEIAGSSAYLAVSGEINLD
                     QETQSLRIKMFPSLGLVTPVIGIASMIADLSLKDPFGRVMFNEYAITGTWDEPLVKKL
                     SEEN"
     gene            3332..4195
                     /locus_tag="PK522_00425"
     CDS             3332..4195
                     /locus_tag="PK522_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNC40227.1"
                     /translation="MLNSTNSFSSSNSDKEENSKIRVAAIQMASGPSVSANLEEALRL
                     IELAVTQQAKLVVLPEYFCIMGMQDTDKLKVREKPGEGVIQTFLSETAKRFRIWLIGG
                     SVPLISQIPDKIFNSCLVYNDAGEQVARYDKIHLFGFSHGNERFAEEKTIKAGDKVIA
                     IDSPFGRIGLSICYDLRFPELYRMMGKIDIILAPSAFTAITGKAHWETLIRARAIENQ
                     AYVIAPAQGGYHVNGRETNGDSMIVDPWGGIMNRLSRGPGVVVAAIDPAYQASVRTSL
                     PALEHRCLYPQ"
     gene            4253..5731
                     /gene="tldD"
                     /locus_tag="PK522_00430"
     CDS             4253..5731
                     /gene="tldD"
                     /locus_tag="PK522_00430"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HNC40228.1"
                     /translation="MVNHKTDMQIDREEHFSIAERCLLTPFQLDTHRLDQVFAAMLSH
                     HIDYADLYFQHNRTEGWVLEEGIVKSGSFNIDQGVGVRALSGEKTAFAYSDDISISAL
                     HSAAKATRAIANQGNSSSARIITHNNDHHPLLYLPHDPITSITDSNKVQLLEKLERYA
                     RTLDNRIVQVLASLSGEYEVIMVIRSDGKLVADVRPLVRVSLQVIAEENGRREQGVAG
                     GGGRFDYSYFTDEILSNYAQQAVHQAIINLSAKPAPAGTMTVVLGSGWPGILLHEAIG
                     HGLEGDFNRKGSSAFSDRIGERVAADGVTVVDDGTLVNRRGSLTVDDEGNPAQNTVLI
                     ENGVLRGYLQDSLNARLMKQPLTGNGRRESFAHIPMPRMTNTYMLNGDRAPEEIIASV
                     KQGLYAVNFGGGQVDITSGKFVFSAAEAYMIENGKITYPVKGATLIGNGPDVLTRVSM
                     IGNDLSLDPGIGTCGKEGQSVPVGVGQPTMRIDGLTVGGTGN"
     gene            5821..7524
                     /locus_tag="PK522_00435"
     CDS             5821..7524
                     /locus_tag="PK522_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HNC40229.1"
                     /translation="MTEELTGAEITIRCLQEEGVEYIFGYPGGAVLFIYDELFKQDKV
                     KHILVRHEQAALHAADGYARSSHKVGVALVTSGPGVTNAVTGIATAYMDSIPLVIISG
                     QVPTAAIGQDAFQEVDTVGITRPCVKHNFLVKDITELAATIKKAFFIASSGRPGPVLV
                     DIPKDVTQQKTHFSYPNSIHMRSYNPVTNGNTKQIKKAAELILSAKRPMFYSGGGVIL
                     DNAAGVLTELVQLLDYPCTNTLMGLGGFPATDKHSVGMLGMHGTYEANMSMQYCDVLI
                     AVGARFDDRVIGNPKHFYSEDRKIIHIDIDPSSISKRVKVDIPIVGSVSQVLKELIKL
                     IKASKEKTDAIALKEWWKQINLWRERDCLKYDRSSHVIKPQMVIEKLYAITKGDAFIT
                     SDVGQHQMWAAQFYPFDKPRRWINSGGLGTMGFGLPSAMGVQFANPTGTVACITGEAS
                     IQMCIQELSTCKQYQLPLKIINLNNRYMGMVRQWQEFFHGNRYSESYMDALPDFVKLA
                     ESYGHVGMKIEKPGDIEGALKDAFKLKKRLVFLDFITDQTENVFPMVPGGKGLSEMIL
                     V"
     gene            7528..8019
                     /gene="ilvN"
                     /locus_tag="PK522_00440"
     CDS             7528..8019
                     /gene="ilvN"
                     /locus_tag="PK522_00440"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNC40230.1"
                     /translation="MRHIISLLMENEAGTLSRVAGLFSARGYNIESLNVAPTEDPTLS
                     RMTLVTIGSDEVIEQITKQLNKLIEVVKIIDLTEGDHIERELMLVKVKAIGDGRDEIK
                     RLTDIFRGSIIDVTDKSYTIELTGTSSKLDAFLETIGQGIILETIRTGASGLGRGDRM
                     LKL"
     gene            8086..9102
                     /gene="ilvC"
                     /locus_tag="PK522_00445"
     CDS             8086..9102
                     /gene="ilvC"
                     /locus_tag="PK522_00445"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNC40231.1"
                     /translation="MKVYYDKDADLSLIKKKKVTIIGYGSQGHAHANNLRDSGVKVTV
                     GLRKDGASWDKAKKAGFTVKEFAASIKDADVVMLLLPDEQIASIYKTEIEAGLKKNAT
                     LAFAHGFNIHYGQVVPREDLDVIMIAPKGPGHLVRSTYTQGGGVPSLIAIHQNKSGKA
                     RELALSYAAANGGTKGGVIETTFREETETDLFGEQVVLCGGLTALIQAGFETLVEAGY
                     APEMAYFECLHEVKLIVDLIYEGGIANMRYSISNNAEYGDISRGPRIIDDKTRAEMRK
                     ILREIQTGEYAREFILENRAGAPMLKSSRRIASEHPIEVVGTRLRDMMPWIKKNKLVD
                     QVKN"
     gene            9217..9870
                     /locus_tag="PK522_00450"
     CDS             9217..9870
                     /locus_tag="PK522_00450"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HNC40232.1"
                     /translation="MKPNYPHPILAREGWPHIAIALLIAASVLVLANWQWSIPFWLLA
                     LFVIQFFRDPARAIPSQGNAILSPADGRIVAVEQTEDPYLKREAIKISVFMNVFNVHS
                     NRSPITGKICEKWYFPGSFLNASLPKASLENERNALWIKTKQGIDVTCVQIAGLVAKR
                     IVCHVKPGDELSAGQRFGFIRFGSRVDVYLPTDIKACVNIGDKVYATTTILAEFKSS"
     gene            9970..10752
                     /gene="pssA"
                     /locus_tag="PK522_00455"
     CDS             9970..10752
                     /gene="pssA"
                     /locus_tag="PK522_00455"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNC40233.1"
                     /translation="MSEPNLTRFGIRSRLRRRGIYLLPNLFTTAALFAGFYAIVQAMH
                     GNYEYSSVAIFIAMVLDGLDGRVARLTHTQSEFGAEYDSLSDMVSFGVAPALVIYEWA
                     LRDMDKLGWIAAFIYCACAALRLARFNTNAEVIDKRFFQGLPSPAAAALVAGLIWVAS
                     DFQVQGTDIKWIAWSITLFAGLTMVSNLPFYSGKEINLRKRMPFFTILLLLMFFFVLI
                     PSHPPVVLFGLFSVYALSGYFMKLWLFSKNKKKSETQEQDVT"
     gene            10991..12523
                     /locus_tag="PK522_00460"
     CDS             10991..12523
                     /locus_tag="PK522_00460"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNC40234.1"
                     /translation="MQDRLIIFDTTLRDGEQSPGASMTKEEKIRIARQLEKMGVDVIE
                     AGFPAASNGDFEAVKAVASNVKDSTVCGLARAIESDIQRAGEALSGASAARIHTFIAT
                     SPIHMKNKLRMSPEQVIEQAVKAIKWASAYTDNIEFSPEDAGRSEIDFLCRVLEAVIN
                     AGAKTLNIPDTVGYTMPEQFGRLIRTLRERIPNSDKVIFSVHCHNDLGLAVANSLAAV
                     SNGARQVECTINGLGERAGNAALEEVVMAVRTRQDYFSCDTRIDTTHIVQASKLVSGI
                     TGFPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMKAEDVGWNANKLLLGKHSGR
                     NAFRTRLNELGIELESEEMLNAMFMRFKELADKKHEIFDEDLHALMSDEAHIPEERYR
                     LLSLMAHSETGEPPFARLVMANGSDELHTESDGSGPVDATFRAIEKILKSGAELQLYS
                     VNNITSGTDAQGEVTVRLQKSGRIVNGHGADTDIVVASAKAYLSAINKLYSKLERAHP
                     QV"
     gene            12529..12993
                     /locus_tag="PK522_00465"
     CDS             12529..12993
                     /locus_tag="PK522_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNC40235.1"
                     /translation="MKHLLCYLFIGLCFITNAIHAEEFAFRFQDSTGKVHKLSDYKGK
                     WVLVNFWATWCPPCLEEIPDLVTLYENRSDIMVFGVAMEYSEVKTVMEFAKALSVTYP
                     IILGNRVIAAQFDELSMLPSTYLFDPKGNPAARKVGLLTRADIEAFIQNYPK"
     gene            13264..16002
                     /gene="secA"
                     /locus_tag="PK522_00470"
     CDS             13264..16002
                     /gene="secA"
                     /locus_tag="PK522_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNC40236.1"
                     /translation="MFSNLLKKIFGSRNDRLVKQYTQVVRAINGLESTIAALSDQDLV
                     NKTQAFKEQIANGQSLDELLPEAFAVVREAGKRVVKMRHFDVQLIGGMVLHDSKIAEM
                     RTGEGKTLMATLPAYLNALSGQGVHIVTVNDYLARRDAEWMGQIYRFLGLSVGVVMSQ
                     MPHELKQQAYAADITYGTNNEYGFDYLRDNMVGHPAERVQRVLNFAIVDEVDSILIDE
                     ARTPLIISGQAEGDTDIYIRINRLIPKLTRQENEKSPGDYSVDEKAQQVLLSEAGFEH
                     AEKLLSDAGLLANDTSLYDPININLIHHLNAGLRAHALFTRDQHYVVQNDEVIIVDEF
                     TGRLMPGRRWSEGLHQAVEAKENVSIQKENQTLASITFQNYFRMYQRLSGMTGTADTE
                     AFEFQQIYGLETVVIPTHNPMIRDDRMDKVYRTMKEKYQAVLEDIKECYQRGQPTLVG
                     TTSIENNELLSSLLNKENLPHQLLNAKQHAREADIVAQAGQPKMITIATNMAGRGTDI
                     VLGGNPEPEINRIRMDESLDEPTKAKQIEEIRKVWQARHEEVVKLGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLALEDPLLRIFASDRVANIMTRLKMPEGEAIEHPW
                     VTRAIENAQKKVEARNFDIRKQLLEFDDVANDQRKVIYQQRNELLEAPQDIGETITAV
                     RESILTSLFNRHIPPESIEEQWDIPGLEKELASDYHLTLPIHEWIEKEDDLQSEDILE
                     RILEIAAQHYQQKSELVGNHIIHQYERALMLHSLDNHWREHLSALDHLRQGIHLRGYA
                     QKNPKQEYKREAFELFGSMLEAIKAEVTKILLTVQIKNEQQVETASEVHTPVNVQYQH
                     ATYEDELTQEQQATPEKASQENKTKPFVRKMDKVGRNDPCPCGSGKKYKQCHGKLK"
     gene            complement(16033..17577)
                     /locus_tag="PK522_00475"
     CDS             complement(16033..17577)
                     /locus_tag="PK522_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNC40237.1"
                     /translation="MNEAIKQSLRQAQEMIRSGDLNGASEILEPLKQTYPELTDVAQL
                     WCAMMMRAGRSGEVLPYAAKIFSLAEGDLQKARWAQMMGTACFQLLELETACYHYMTA
                     LNHLMALTKSGKVPADQKSRTVKESTENIFLSGRAERLLWETCAALAANGIRAFPFAG
                     TLLGIVRHGHLLAFDKDLDIAVWMENWDDCCALLEKMGWQRTPMSFNYRNYRDYVHSE
                     IGITLDLCGLQLNKANRIIGGFSLPGYPDEYQRVSIFPKFDLIPKDTPYGTVWFPNPP
                     ETILTAFYGNWRTPNPYWDTVVSAVNLEKFTLLVRCFAYYRLIQRWLSGELVRAWSYA
                     HQIALKDPDDVISLRSRQWLERALTRLNQAIPEWPSNRSQKRVYTRMVADLFHVGHVN
                     FLREAKALGTHLTVFVVSDQRVQENKGKHPVMSQSERAAVVSACKYVDAVVTEGPLYA
                     TPAFMQQQGYDLYTFACSSEKERQEKYKLCAELPASMIKEIDYTDGISTTDLLTRITI
                     QDRNVS"
     gene            17715..18329
                     /locus_tag="PK522_00480"
     CDS             17715..18329
                     /locus_tag="PK522_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulating protein"
                     /protein_id="HNC40238.1"
                     /translation="MAITKEPRPNLSNSGNIFEIIDGIQFLGFGSGGFDANDLLIIGD
                     ELDNLLEGNNGADDHDEIEGGAGNDRLLGLNGNDTLDGDEGNDDLNGGEGTDKLFGGL
                     GDDILRAGFGHDRLTGNDGSDTFGFYAAGFYQVKDFSITQNDRLFFDSQTLGINSLEQ
                     LVTYITNIEQRADGFKVYFGEVATIDIVGANLSAITADMIVFNL"
     gene            18484..18669
                     /locus_tag="PK522_00485"
     CDS             18484..18669
                     /locus_tag="PK522_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HNC40239.1"
                     /translation="MYHSYMHLQTSNNSDHSAANDLRKSINSDALFTGNNEIVIQHRE
                     QQYRLRLTRAGKLILTK"
     gene            18861..19466
                     /locus_tag="PK522_00490"
     CDS             18861..19466
                     /locus_tag="PK522_00490"
                     /inference="COORDINATES: protein motif:HMM:NF021593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HNC40240.1"
                     /translation="MKLLKFPLSQTIYCDNSHNWVNWQQISHITNTKLKNIQNWITVV
                     SAKIFRSHALRSSTPLTTRVIPLIKEKQINPRLLIFANYKPFNKTEGLAGKIILCIGG
                     RIALYSDYQKLVEIAGGNFIGYRGADMQSDQLKKLLDQANMVICPVDCINHEDFFFVK
                     NYCAITGKICTFLARSTLLNFQQGLKILINLSHHNLGTVVS"
     gene            19463..20449
                     /locus_tag="PK522_00495"
     CDS             19463..20449
                     /locus_tag="PK522_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNC40241.1"
                     /translation="MKNKKIDAVSLFIAFLLGVFFILSSQLEAKTIREYWIAAEKVTW
                     NYAPSGKNLIEPEGNLGVWGETLTYTKYQYVGYTDGSFNAPIAQPEWAGLLGPQIRAV
                     VGDTIKVHFLNKTDRPLSIHPHGIFYKKEDEGADGGKGSSVQPGERYTYTWIADEDAG
                     PGPSDPSSIVWLYHSHVMAEEETNLGLIGTLIITRKGKERSRDDPTPKDVTQEFIALY
                     TIFDEENGEEPGLKHAINGRIFGNLAGYQTQLGSRVRWYLVALGNEVDNHTVHWHGQT
                     VLHRGHRTDVIELMPASMTTVDMVPRSAGNWLFHCHVNDHMMAGMSTYWHVK"
     gene            20570..20930
                     /gene="ssrA"
                     /locus_tag="PK522_00500"
     tmRNA           20570..20930
                     /gene="ssrA"
                     /locus_tag="PK522_00500"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(21101..21286)
                     /locus_tag="PK522_00505"
     CDS             complement(21101..21286)
                     /locus_tag="PK522_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40242.1"
                     /translation="MNKEMKWKYNGTRTNDSDEIHIARNAVITIDESFKESVLALIQA
                     VKRLSLAECKLVEVKIK"
     gene            complement(21449..22777)
                     /locus_tag="PK522_00510"
     CDS             complement(21449..22777)
                     /locus_tag="PK522_00510"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNC40243.1"
                     /translation="MQRFIGEAPEKIIDSSTTLFWLRRDLRLEDNAGLFHALNENSTV
                     LALFIFDTVILERLEDKKDARVEFILQSLLILKAALEKLGTSLFVYHGNPTEFFKRVN
                     PRSVYANHDYEPYARQRDNEVQQILAKREIAFKTYKDQVIFDRDEIIKADSSPYTVYT
                     PYSRKWKATLTPAHIENYDTRHLFDHFKKIAPLKFPTLSDLGFGKSGIVFPERTIKRS
                     VIEKYHLQRDFPAIESTSRLSVHLRFGTVSIRMLVQVAMTINEKWLNELIWRDFYHMI
                     LWHFPHAATKSFKPAYDQIPWRTDLTEFSAWCEGQTGYAIVDAGMRELNATGFMHNRA
                     RMITASFLVKHLLIDWRWGEAYFAKKLLDFDLAANNGGWQWTVGSGCDAVPYFRVFNP
                     MLQAEKFDPDQRYVRKWVPELGTPQYPKPIVDHAVARDRALKIFKAALST"
     gene            22858..26055
                     /locus_tag="PK522_00515"
     CDS             22858..26055
                     /locus_tag="PK522_00515"
                     /inference="COORDINATES: protein motif:HMM:NF023167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HNC40244.1"
                     /translation="MLLMVSFLYLRNAVNLLILSVAQCRILLPIPTVLIFFKLVKRLS
                     IWFLIFCSGLALLASILYFSRYALLEALLIHQLQAKNVPLQSFTIRNISLNQVLIRDL
                     SLGTHEELRIDQIDINWSLIDLLKGKLDVVSLKGITIWLDFSGQQPVLGSLHPLLDSP
                     SNDKNGPVTIQLPAIAKLQADIDFRSAQGNLRLNLFGKINQREALTQSAVIEFGLSGF
                     SIKTNGQFNVTIDSTQTIQGLIAIADGSINRPDLEIAKISGETTFALLAMQLRQFKAE
                     LNLDEILFPESQQSVVQAYKQASLDLQLIENHIQLTGALLTTNNAPVLDIQITANKPQ
                     DSNDFDIAMNASGRLSYLPWNLGGREQPSSGTFSLGLQGSGQIPSNVSQSGDSSWLEK
                     IQFEGNAQLDCQALSYETKLLNLSGSLGFKVAINKGMGQANLTPDSIIQLGSADPAWL
                     TQSGLPPALSKDLAKGGTLRFNQTKQDQLSQVNWKLQSKQVDIRPQLFASLRFAKTRA
                     DFMGHGQVELKLNESKAAHGIIDSWNGNFNLSTQIDHMDLGSLTVHKMLMNIPMRTSA
                     CGKNWSVALTEPGQIRFGLSDKTKQFSIKQPLHLVISQLDIDAKQRIDDFSIKHQVSA
                     TLSPFSAWMMSQIEPIEIKIHPGRFALSGQLDKDQPYQAEGKWTGATALLPQSQIKLE
                     NIVASLSLGTKKRALAQLAIGKLYHLADAPFFAPHTLSATINDKTKGKQHIYTLDVSG
                     GLPNLKYFHLKAEQNLDNGFGKLKFNLTPIHFIPGGTQLSDLLPGLGQITDMQGVVHA
                     HTQVDWSKKGVQKSGGAIVADNLSFTHPTFSIAGLRTRIDFDQLLPINTPPQQQIHIK
                     TIDAGIPLSDLNIIYQIESNRNNQPRIKLEDTQLAILDGILTIEPVTIDPLSRDTKIT
                     ITLNNINLKRFFDFIQIEGLAGEGRLDGRVPILLTDSRITIDKSRLMAKSPGILRFQS
                     EKASQMLANAGTEMNLVLDVLEDFHYSELSLSIDKSAEHDLTATLSLLGQNPAVKEGQ
                     MIRLNINLASNLDKILKAISLGYNVSNEILKDLFR"
     gene            26160..26375
                     /locus_tag="PK522_00520"
     CDS             26160..26375
                     /locus_tag="PK522_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HNC40245.1"
                     /translation="MHNIIRLGDQVLAVASLSLFVMMIVACTPTVKVESPQEPITINL
                     NIKLDADIRVKLEEEAKKDIAANPDVF"
     gene            26404..26754
                     /locus_tag="PK522_00525"
     CDS             26404..26754
                     /locus_tag="PK522_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HNC40246.1"
                     /translation="MPPLLKKTTKLNLLGIINSILLLGLLYAFPAWSDNLEQLRASGM
                     IGENAGGFVVARDPSIQGEVEAINAKRKAIYQEKATAQGVSPEQVGKVYASEIFKKVP
                     AGTWIQLNGQWVKK"
     gene            26843..27403
                     /locus_tag="PK522_00530"
     CDS             26843..27403
                     /locus_tag="PK522_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNC40247.1"
                     /translation="MIRENLIKALIAIGVFVVLMAALGFLFEDELATATSWVVERIGF
                     AGLCMILLVTDTFVTPFPPDILLIVIAKSSLSEQWPFYVLILSMVSVFAGMLGWGIGR
                     WLGHFQFAKRLFGEFKDEHREFIRRYGFWAVVIGAITPLPFSVTCWASGVMGLRAVTV
                     FSAAALFRIPRFFIYYWIIASTSQWF"
     gene            complement(27427..28068)
                     /locus_tag="PK522_00535"
     CDS             complement(27427..28068)
                     /locus_tag="PK522_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40248.1"
                     /translation="MIRDQFKEYPVAVSYKNIRAELDTGDIVVFGGQYRMSKIIRLVT
                     RFPASHVASIIRENDRISFIEASEGDMYPEREGVIISQFSNSIPFYKGDIWIARLSAE
                     IRRKADIGKMKAFLQEQVGKGYDYKNMPKAGFDFLDRIGITRSEENLSQMFCSELVAA
                     GLKAAGIFPEDINVSEVSPADIVKYKIFEPTYYQIKAFNYKAIKLPHFNTVVI"
     gene            28557..29765
                     /locus_tag="PK522_00540"
     CDS             28557..29765
                     /locus_tag="PK522_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNC40249.1"
                     /translation="MTQANAYEQYMLELINIERAKVGSQPLTFDGDLNESAENHSTWM
                     IATDTFSHTGVSGSSPGDRMKTAGYIFSGSWSWGENIAWMSTRAPTGLQDEVLQLHTM
                     LMNSSGHRANILNNNFREIGVGFEVGQYQAYEGAFVTQNFAKTNTNPFLTGVAFDDRD
                     DDLRYDMGEGLSNFTVSAKNNTTGAITTTKTTLAGGYELELAAGNYTISFSAPGFTTT
                     TKQASISTKNVKLDLIDPVGSNTINGTSGPDTLQGTTNSDIIFGSGGNDIINGNAGND
                     RIDGGTGNDRIIGANGSDTLTGGSGQDTFVFNTPISGGIDRISDFSSLDDVIHLARST
                     FSGLSTGQLPIAAFHIGTGAHDSTDRIIYNSSTGALLYDADGLGGTGSQLFAQLSPGV
                     IVTNTDFYII"
     gene            complement(29861..30448)
                     /locus_tag="PK522_00545"
     CDS             complement(29861..30448)
                     /locus_tag="PK522_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HNC40250.1"
                     /translation="MLLEKPFNDDEQPINSGINRRFFIKAGLGACIGLVLPQAAYANY
                     TKKTGIRQISFHNLHTGEKVKAVYWEKGRYINGALREIDNVLRDHRTGKRHAIDVRLL
                     DMVQLLHRLLGAKKEFQVVSGYRSPETNRLLAAQRYGVAKHSLHMQGKAIDIRLPEFS
                     LSDVRKAALSMQVGGVGYYPGSNFIHLDSGQVRNW"
     gene            complement(30490..32145)
                     /locus_tag="PK522_00550"
     CDS             complement(30490..32145)
                     /locus_tag="PK522_00550"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNC40251.1"
                     /translation="MERISSFQQLRFSIAFTYKILLCLISILVLLLVTTGNHFAQANA
                     FVLQEKLAHIPVTTANETSLTMLKRFYVKRDYQPIWWGSSSTSGISIDKVISFIGSAE
                     QHGLDNQDYELNKLIQLQKRDGVDSPSLELELTAIQSLLMLAKDLRSGRLLASKADPD
                     WHIEQHRFDPVDYLNDAIRENHLARSLVELPPQSLHYQLLKTALAKYRDLEVQQQNWQ
                     SIPESAAIRPNTLHKSIPLVRKRILRLYNIQNDPKYGIVKIDNERYDSGLVEAVKQFQ
                     EQHGLNPDGIIGKQTIIALNRTPKQRIQQIRANMERLRWMPRNLGERHVMINIAGFYL
                     SAIEGGKHVLEMRIIVGRDYRSTPSFDSQLSQMVLNPYWNVPQSIAVKDLLPKQQQNS
                     AYFASQGIKVFSRHENVPINPDSINWNSIKGGFPYVLRQNPGKKNALGYIKFLFSNPF
                     SIYLHDTPSKQLFQKDVRTFSSGCIRLEKPWELARFALNGNNSGINLTEKINSGETIT
                     INLTKRVPIYLTYLTAWVDDQDHIHFAEDVYGRDKRLLEYARW"
     gene            complement(32250..33212)
                     /locus_tag="PK522_00555"
     CDS             complement(32250..33212)
                     /locus_tag="PK522_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC40252.1"
                     /translation="MNFPNTIDFPHAISAIDAQYHRPGRAAIHLIVEKGVAAIVDTGT
                     KFSIPGVIAVLEHKQISLENVAYVFLTHIHLDHAGGASELMRLLPNTQLVVHPRGAKY
                     MINPAKLIAGVKAVYGEAEFARLYGEIYPIDADRVVEAPDETCIELNGRQLLLLDTPG
                     HARHHYCIFDAQSQSFFTGDTFGVSYRELDVDGLEFVFPTTTPVQFDPDAAHASIDRI
                     MSYHPQFAYLAHYSRIGNLSHHAQQMHALIDEFVHIANRAVTQNERHLYMVQAMQALL
                     ERRLQAHGCKLRPDAIFDLLKVDIKLNTLGLETWLDQAETSNKS"
     gene            complement(33261..35231)
                     /gene="acs"
                     /locus_tag="PK522_00560"
     CDS             complement(33261..35231)
                     /gene="acs"
                     /locus_tag="PK522_00560"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNC40253.1"
                     /translation="MAAIESVLHETRIFPPSVEFVQQANISGNESYRALCAEAENDYL
                     GFWSELAKQHIMWHKPFTKILNDSDAPFFKWFEDGELNISYNCLDRHLRDQPDKVAIR
                     FEADDGKVTHCTYRELYHQVCQFANGLKALNIKKGDRVIIYMPMRIEAVVAMQACARI
                     GAIHSVVFGGFSAKSLHERIVDAGAIAVITADEQTRGGKQHPLKLTVDEAISMGNTES
                     VQSIIVYKHTGTSVPWQNGRDIWWHELIQDKALDCDPEWVNAEHPLFTLYTSGSTGKP
                     KGVQHSSAGYLLGTIVSMQWIFDYKPSDIFWCTADVGWITGHSYVAYGPLAVGATQVI
                     FEGIPTYPDAARFWRIIQNHQVTIFYTAPTAIRSLIKLGVDLPKQYDLSSLRLLGSVG
                     EPINPEAWMWYYTVVGQSRCPIVDTWWQTETGCHMIAPTPGAMPLKPGSCTFPLPGIS
                     VAIVDEAGHDVEQGKGGFLVIKRPFPSQIRTLWGDPARFKKTYFPHDIGDGRYYLAGD
                     SAHRDKDGYFWIMGRIDDVLNVSGHRLGTMEIESALVANPLVAEAAVVGKPHEIKGEA
                     VVAFVVLKEKSPQGEQAQEIAHILRDWVAKEIGPIAKPEEIRFGDNLPKTRSGKIMRR
                     LLRSLAKGEEITQDTSTLENPAILDQLKQSIN"
     gene            35406..36815
                     /gene="leuC"
                     /locus_tag="PK522_00565"
     CDS             35406..36815
                     /gene="leuC"
                     /locus_tag="PK522_00565"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNC40254.1"
                     /translation="MKTLYDKLWEAHVVHAESDKPDSTVLLYIDRHLVHEVTSPQAFE
                     GLKLAGRKPWRTRSILAVADHNVPTTNRKNGISDPTSQLQIETLDQNCDELGITEFKM
                     NDLRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFACLAFGIGTSEVEHVLATQCL
                     VAKKSKSMQVLVEGVLGKGVTAKDIALNIIGKIGTAGGTGYAIEFAGNAIRSLSMEGR
                     MTLCNMAIEAGARAGMIAVDDTTIDYIKGRPFAPKGQMWESAVNYWRTLKSDEGAVFD
                     CVVTVNAAEISPQVTWGTSPEMVTSIEGSVPDPESIFDLSKRNDVESALKYMGIKPNT
                     PIQAIYLDKIFIGSCTNSRIEDLREAASILKGKRIADNIKLAIVVPGSGLVKSQAEQE
                     GLDQIFRAAGFEWREPGCSMCLAMNDDKLLPGERCASTSNRNFEGRQGPGGRTHLVSP
                     AMAAAAAVAGHFVDVRLIH"
     gene            36853..36978
                     /locus_tag="PK522_00570"
     CDS             36853..36978
                     /locus_tag="PK522_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HNC40255.1"
                     /translation="MKLFILLSAFLMTLCLTGCNTLQGFGKDIQRGGEAIERTAQ"
     gene            36987..>37248
                     /locus_tag="PK522_00575"
     CDS             36987..>37248
                     /locus_tag="PK522_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNC40256.1"
                     /translation="MKKFTDCDGLAVPLDRSNVDTDAIIPKQFLKSIKRSGFGQNLFD
                     EWRFLDTGEPGKENLHRKLNPDFVLNFPRYQGARILVTRDNFG"
BASE COUNT        11197 a         7888 c         7781 g        10382 t
ORIGIN      
        1 atcgtttagg gttgcgagtc gctgccttgg atagcactgc gatgtcgatg gatattcggg
       61 gcgatttcat aggcaaatcc attgaatcac ttccacattg gcaagggcgc ctctactttg
      121 aattaaagca aattgatcta gccgtttggc atcgatggat tccattccct ggcgaaatcg
      181 atatcaaaca aggtaaaggt gctctaagag gctggctaac cttaaccgag ggcaccatca
      241 atgactggtt gattgatgcc gatttgcatc gaatcaacgc aagccttgac acaaacttac
      301 ccaaactcga actcgatttc ttgcaaggac gtattagcgt caagcaatct aatcaatccg
      361 ggagcctagt ccaagaatgg acggcacacc aattaaattt caaattagcg catcaacaaa
      421 tcgtaacaaa cccaatcaat gcgatatggc gtcaacacca tgatccaaag ccaagtgaag
      481 catcaaatta tctgcaaatc gatgaaatcg acgttggggt gtgggctaat ttacttgagt
      541 atttgccggt agatgcatca gtgcgtaagc aagttgaaaa gtactcaccg catggaaaaa
      601 ttgaacacat gcagctcagt tggaaaggaa gttggtcaga accacacgca tatcaactcg
      661 atacacaatt caatgattta tccttaaata acattcagat gggacattta atctcaatcc
      721 atgggctcag tggaaacatt caagggactg agcaagaagg gagactcaat ttaaattccc
      781 agtccgtctc tttgcaatta cgacagccgt ttccacaatc gattgtattt gataccttag
      841 ccacaagagt aggttggaag aaaatagcag aacaagaaag taccacttac aaaatcgaag
      901 acctagcatt taacaaccaa catttagcag gatccgccat tcgtggcagc tatgtacaca
      961 aacaaaataa gcctgactgg attgatatac aaggcaattt aaaccaggcc gacgttaacg
     1021 gtctctctcg cttattatta cctttcacta acaagactca actagcctgg atcgatgagt
     1081 catcattatc agggcaatta aaaaatacgc aatttaatat ccaaggtaat ttaagcgatt
     1141 cttccgcaaa cgatttttcc tggtcatttg ctaccgaaat ggtcaatata tcagctagta
     1201 ttggagcgca atggccaagc attaccaata tttcagggca cttgacggct agctctacac
     1261 tgattaatgc aattgtgtca gaaggcaata tcgaaacgat tcagttcaaa gatacttcca
     1321 tccatatcgc tgatcttcag tcaaaaaagc cgcttctaaa aataaaaggc gaagcaacag
     1381 gaaaaacaaa acaaatcatc caattgataa aagaatcacc actaacgcag aatacatttg
     1441 atatcgatat tccaggcgtg ataactggca agggaaagtt acaattcgaa tcaaccatcc
     1501 ccatcctgtc aaaagaaaaa attgttgata tcgatcttaa aggtcagtat caatttatca
     1561 acaatcaaat cgatttgggt cccgagctac ccgctatcag taaagtcaat ggaaaggttt
     1621 cctttactca taacacatgg gattttgatg aaattcacgc acaacttctg ggcgggccat
     1681 tagtaatccg ctctaagcct ctcaacaatg ataaaaaaca aattgcctta gaaggtcagg
     1741 caaatttcga ccgcttcaat acacgcctat cccaaaaatc accgagtgct ttgcagtttt
     1801 ggctacagca tattcacggc acgactgact ggacagcgat catcgatatg aacaatagtc
     1861 aaataacggc cctgcagata gcatcatcac taaacggtgt tacttcggta ttaccagctc
     1921 ctttttctaa aacacacgat gaaataattc cactcacttt tactaaaacc gcgatcgact
     1981 cagcaaacga aaagttattt tgccaattag gagaaaaagt caccactgag attcaccgca
     2041 cccagacaaa acagggagat ttcattccgg tgcgtggtgt catgagtttt ggcaatcgtt
     2101 cagcaacgtt acccagcgaa aaattgacat taatgcatgg ccctattcct ttactcgagt
     2161 gggataaatg gcaagaaatg cttgatcaac acgaagcgat cgttgggctg tcaaagcagc
     2221 aaagtcgcgg tatcgatgct ttactcacac atcacatacg gtttgattta aatgtggggg
     2281 tccttgattt cttaggaagt cgattcaatg attttatttt acatgcggat aaacagggtt
     2341 cagagtggac taccgacgtg accagtcgag aagttaccgg gaaaatctac tggaatgcga
     2401 caggcaaaaa aaaggcaacc gcgaggttaa aaaaattagt tgtacctgaa tctcttccgg
     2461 aaaacggttc agccgagaga aaaaagcacg agtctcagaa ttggccgatt atcgacttac
     2521 atgcagatga atttttttac aaagaaaagc tactcggtca attaacgtta ttagccgagc
     2581 aaaacaaaga aaattgggta gttgatcaaa tggaaatctc ccacaagcac agtatcttca
     2641 ctgccaaagg catctggaaa aaccggcagg aacctttttc aatgtatgcg gagatcaaat
     2701 taacgacgag cgatatcggc aaattcctca accgcgttgg ttaccccgat cgcattgctc
     2761 gtggcgaagg aagcttggag ggccacctgt catggcaagg gaaaccattc tcagttgatt
     2821 tccccacctt atcagggcaa ctgaagctag ctgctaagca tgggcaattt actaaattca
     2881 aacccggcat gagcaagtta ttaggcattt tcgatctcaa agcactgcca cggagactga
     2941 cacttgattt ttatgatatt ttcagtcaag gttttggttt cgatgatatt cttggaaatg
     3001 ttagaattga tcacgggatc gcaacctttg tagatttaga aattgcaggg agctctgcct
     3061 atctcgccgt ttcaggagaa attaaccttg atcaggaaac acaatcattg cggattaaaa
     3121 tgtttccatc gttggggtta gtcacaccgg tgatcggaat cgcttcaatg attgcagatc
     3181 tttcactcaa agatccattt ggaagagtga tgtttaatga atatgctata acaggaacat
     3241 gggatgaacc gctggtcaag aaactatctg aggaaaacta ataaaacatg aatacgctac
     3301 aagctatatc tgctgttttg ggagtcagaa tgtgttaaac agcacaaatt ctttttcttc
     3361 atcaaattca gataaagaag aaaactccaa aatacgggtt gcagcaatcc aaatggcttc
     3421 cggccccagc gtctcggcaa atttggagga agcgctgcgc ctaattgaac tcgcagtcac
     3481 acaacaagca aaactcgttg tactacccga atacttttgc atcatgggaa tgcaagacac
     3541 ggataaactt aaagtccgcg aaaagccggg ggaaggagta atacaaacct ttttgagcga
     3601 aactgccaaa cggttccgaa tatggcttat tggtggatca gttccgctca tctcccaaat
     3661 acccgataaa atttttaata gctgcttagt ttataatgat gccggcgaac aagttgcccg
     3721 ctatgacaag attcatttat ttggtttctc acacggtaat gaacgttttg cagaagaaaa
     3781 aaccattaaa gcgggtgata aagttattgc aattgactcc ccatttggtc gaattgggct
     3841 ttcaatctgc tacgatctaa gatttcctga gctctaccga atgatgggga agattgatat
     3901 cattttagct ccgtccgctt ttactgccat taccggaaaa gcacattggg agacattaat
     3961 tcgcgcacgc gccattgaaa accaggctta tgtgattgca cctgcgcaag gtggatatca
     4021 tgtaaatgga cgcgaaacta acggagatag catgatcgtc gacccttggg gcggaataat
     4081 gaaccgctta tcccgcggcc caggtgtcgt tgtcgcagct atcgatcctg cttatcaagc
     4141 cagcgtaaga acaagcttgc cagcacttga gcatcgctgt ctctatccac aatagatcaa
     4201 agcgtgaacc tattaataca aagtaaagca gtaataaaaa atactagata acatggtaaa
     4261 tcataaaacg gatatgcaaa tagatagaga agaacacttc tctattgcag aacgctgcct
     4321 actaactcca tttcagctgg atacccatcg gctagatcaa gtatttgcag caatgctatc
     4381 gcaccatatt gattatgctg atctttattt tcagcataat cgaaccgagg gttgggttct
     4441 tgaagaaggt attgttaagt cgggcagctt taatattgat caaggtgttg gtgttcgcgc
     4501 tctaagtggc gaaaaaacag cctttgcgta ttccgatgat atcagtatct ctgccctaca
     4561 ctctgcggcc aaagcaactc gtgccatcgc aaatcaggga aattcatctt ctgcgcgaat
     4621 cattactcac aataacgatc atcatccttt gctttactta ccgcatgatc cgatcacaag
     4681 cataaccgat agcaataagg tccaattact tgaaaaactg gaaagatacg cacgtaccct
     4741 cgataatcgc attgtccaag tattagcctc cttatccggt gaatatgagg tcatcatggt
     4801 aattcgtagc gatggtaagc ttgtcgctga tgtaagacca cttgtacgag tctcccttca
     4861 agtaattgcc gaagaaaatg gaaggcgtga acaaggtgtt gcagggggag gcggtcgttt
     4921 cgattactcc tatttcacag atgaaatatt gagcaactat gcacaacaag ccgtgcatca
     4981 agcaatcatc aatttgagtg caaaacctgc gccggctggt acaatgacgg ttgtgcttgg
     5041 tagtggatgg cctggtatct tattacatga ggcaattggt catggattag aaggcgattt
     5101 taaccgtaag ggtagctctg ctttttctga ccgcattgga gaacgtgttg ctgcagatgg
     5161 tgtgacagtc gttgacgatg gaaccctggt caatcgaaga ggatcgctta ccgtagatga
     5221 tgagggaaat cctgctcaaa atacggttct tattgaaaat ggggtactcc gaggttattt
     5281 acaagatagc ctaaatgctc gattgatgaa gcaacccctt accggcaatg gaaggcgcga
     5341 atcctttgcg cacatcccta tgccacgtat gaccaatact tatatgttaa atggagatag
     5401 agcccctgaa gaaattatcg cctcagttaa gcaaggccta tacgcggtca attttggagg
     5461 ggggcaggtt gacattacga gtggaaaatt tgttttttct gctgcggaag cgtatatgat
     5521 agaaaatgga aaaatcacct atccggtaaa aggagcaacc ttaattggaa atggtccaga
     5581 tgttctcaca cgtgtttcga tgatcgggaa tgatttgtcg cttgatcctg gtattggaac
     5641 ttgtggaaaa gaaggtcaaa gtgttccagt aggcgttggg caacccacaa tgcgtattga
     5701 tggattaact gtaggtggca ccggcaacta ggtagtaaag taaattctaa ttttacattt
     5761 ataatcagct ttttaatgaa gcaaccctaa gaaaaatatc aataatagga tgcaatagga
     5821 atgaccgaag aattaactgg tgcagaaatt acgatccgtt gcctacagga agaaggggta
     5881 gagtacattt ttggctaccc tggcggtgct gtattattta tttatgatga gctatttaaa
     5941 caagataaag ttaaacatat cttggtcagg cacgaacaag cggcattgca tgctgccgat
     6001 gggtatgcac gatctagtca taaagtgggt gttgcacttg taacgtcagg accgggtgta
     6061 accaatgcgg ttacaggaat cgccaccgcc tatatggatt caattccatt agtcatcatc
     6121 agtggtcaag taccaactgc tgccattgga caagatgcct tccaagaagt tgacacggtt
     6181 ggaatcactc gcccgtgtgt caaacataat ttcttagtta aagatattac tgagcttgct
     6241 gccacgataa aaaaagcttt ttttattgct tccagtggaa gaccaggccc tgttctcgtg
     6301 gatattccta aagatgtgac acagcaaaaa acacacttta gctacccgaa tagtattcac
     6361 atgcggtctt ataatcccgt caccaacggc aatacaaaac agataaagaa agcagctgag
     6421 cttatcctta gcgcgaaacg ccctatgttc tattcgggtg gcggtgtcat tcttgataat
     6481 gcagctggtg tattgactga attagtacaa ctgctggact atccctgtac aaatacatta
     6541 atggggctgg ggggatttcc agcaacggat aagcattccg taggaatgct cggcatgcat
     6601 ggcacttatg aggccaatat gagcatgcag tattgtgatg ttttgattgc tgttggcgca
     6661 cgttttgatg atcgagtcat tggtaaccct aaacactttt acagcgaaga tcggaaaatc
     6721 attcatatcg atattgatcc ttcctcgatt tctaaacgcg tgaaagtaga tataccgatt
     6781 gttggtagtg tttctcaagt attaaaagaa ctcattaaac tcattaaggc cagtaaagag
     6841 aaaacagatg ccattgcatt aaaagaatgg tggaaacaaa tcaatctgtg gcgtgagcgt
     6901 gattgcttaa aatacgatcg ttcaagtcat gtcattaaac cgcaaatggt cattgagaaa
     6961 ctctatgcga tcacaaaagg agatgcgttt atcacctcag atgtgggtca gcatcaaatg
     7021 tgggctgctc aattctatcc gtttgataag ccacgccggt ggattaattc aggtggatta
     7081 ggtacgatgg gatttggctt accttccgcg atgggggttc aatttgccaa ccctacggga
     7141 acagtcgctt gcataacagg ggaagcaagc attcaaatgt gtatccaaga attatcgact
     7201 tgcaaacaat accaactccc attgaaaatc atcaacctta ataatcggta tatgggcatg
     7261 gttcgacaat ggcaagaatt ttttcatggc aatcgctact cagaatctta tatggatgca
     7321 ctacctgatt tcgtaaagct tgctgaaagc tacgggcatg ttggcatgaa aattgagaag
     7381 cctggtgata ttgaaggtgc tttgaaggac gcgtttaaac taaaaaaacg cctcgtcttt
     7441 ctagatttta ttacggatca aacagaaaat gtattcccga tggtgcctgg tggtaaaggt
     7501 ttatcagaaa tgattttggt ataaaaaatg cgacatatta tttctttatt gatggaaaac
     7561 gaagcaggca cgctatcgcg agtggctgga ttattttctg cacgcggtta taacattgaa
     7621 tcactcaatg ttgcgccgac ggaagatccc actttatcgc gaatgacatt ggttacgatt
     7681 ggatctgatg aagtcattga acagattact aaacaactca acaaattaat tgaagttgtt
     7741 aaaataattg acctcacaga aggcgatcat attgaacgcg aattaatgtt agtaaaagta
     7801 aaagcaatcg gggatgggcg agacgaaata aaacggctaa cggatatctt ccgcggaagt
     7861 atcatcgatg taaccgataa gtcctatacc attgaattaa ctggaacaag ctcgaagtta
     7921 gacgcatttc tagaaaccat cggtcagggt ataatcttgg aaacgattag aactggtgcg
     7981 tcaggtctcg gtcgtggaga tcgtatgctt aaattgtaac taaagtaatt ttgcttcagt
     8041 ctttttgtat ttttgatgct agatattaat aaataaagga tcacaatgaa agtctattat
     8101 gacaaggatg ctgatttatc cctgattaaa aaaaagaaag taacgattat tggttatggc
     8161 tcacaaggtc atgcgcatgc caataatctt cgtgactcgg gcgttaaggt aacggtaggg
     8221 ttacgtaaag atggtgcttc ctgggataaa gctaaaaagg caggttttac cgttaaagaa
     8281 ttcgctgctt caattaaaga tgcagacgtc gtcatgctgt tgctcccaga tgagcaaatc
     8341 gcatcgattt ataaaaccga aattgaagca ggattgaaaa aaaatgcaac gcttgctttt
     8401 gcccatggct tcaatattca ctatggtcaa gttgtaccac gagaagatct cgatgttatc
     8461 atgattgcgc ctaaagggcc tgggcatctt gtcagatcaa cttatacgca aggtggagga
     8521 gtgccctctc ttatcgcaat tcatcaaaac aaatcaggta aagcacgcga attagcatta
     8581 tcgtatgctg ctgccaatgg cgggactaaa ggtggtgtaa tcgaaaccac attccgtgaa
     8641 gagactgaaa ccgatttatt tggagagcaa gttgtactat gtggtggtct taccgcatta
     8701 attcaagctg gatttgaaac attggttgaa gcgggctatg ctccagaaat ggcttacttc
     8761 gagtgcctcc atgaggtcaa gctcattgta gatttaattt acgaaggtgg tatcgccaac
     8821 atgcgttatt cgatttccaa taatgcagag tatggtgata tttcaagagg acctcgaatt
     8881 atcgatgata aaactcgagc tgaaatgcgt aagattctcc gtgagattca aaccggtgaa
     8941 tacgctcgcg aatttattct agaaaaccga gctggcgcgc ccatgttaaa atcgagccgc
     9001 cggatcgctt ctgaacatcc aattgaggtc gttggaacga gactccgcga catgatgcca
     9061 tggatcaaaa agaacaaatt agtcgatcaa gtaaaaaact aatcatcgct cattcaattt
     9121 attgatagat ttactttttc acttgttttt tcgcgcttgc taacccggta ttcttcacaa
     9181 tatcgggttt attattttta gtatgatgaa attgctatga aaccaaatta ccctcaccct
     9241 attctcgccc gcgaaggatg gcctcatatt gctatcgcat tactgattgc agcttctgtt
     9301 cttgtcctag ctaattggca atggtcaatt ccattttggt tgcttgcttt atttgttatc
     9361 cagttctttc gtgatccagc gagggccatt ccttctcagg gaaatgcgat attatctcct
     9421 gctgatggaa gaatcgtcgc agtagaacaa actgaggatc cttatttaaa gcgagaagca
     9481 atcaaaatta gcgtattcat gaatgtattt aacgtacatt caaatagaag cccgattact
     9541 ggcaaaattt gcgaaaaatg gtacttccca ggaagctttc tcaatgctag cctccccaag
     9601 gcttcacttg aaaatgaacg caacgccctc tggataaaaa cgaaacaagg gatagatgta
     9661 acttgcgtac aaattgcagg gttagtcgca aagcgtattg tttgccatgt taagccagga
     9721 gatgaactct cagctggaca acgttttgga tttattcgtt ttggctctcg agttgatgtt
     9781 tatttaccca cagatataaa agcttgcgtt aatattggtg ataaagtata tgcaacaaca
     9841 actatattag ctgaatttaa atcatcataa ctaaaacgat tagttgtaac ctttctagtt
     9901 gatactttaa gaatttattg atgttttata ttgatgtttt attaggcgct tatcttgatg
     9961 ctgtgagcaa tgtctgaacc caatcttact cgatttggta ttaggtcaag actaagaaga
    10021 cgtggaattt acctattacc aaatttattc acgacagctg ccttattcgc gggattctat
    10081 gccattgtgc aagcaatgca tggcaattat gagtattcct ctgtagctat ttttatcgca
    10141 atggtactcg atggtctaga tggtcgtgtt gcacgactaa cgcatacgca aagtgagttt
    10201 ggtgcggagt atgatagctt gtcggatatg gtttcgttcg gggtcgctcc ggctctggtc
    10261 atatacgaat gggcactccg tgatatggac aaactcgggt ggattgccgc ttttatctac
    10321 tgcgcctgcg ctgcactaag actcgcacgc ttcaatacca atgcagaagt gatcgacaaa
    10381 cgcttctttc aagggttacc tagtccagct gctgctgcct tagttgccgg attaatttgg
    10441 gtcgcatcag attttcaagt acaagggact gatattaaat ggattgcatg gtctataacc
    10501 ttatttgctg ggttaacaat ggtcagtaat ctacctttct atagcgggaa agaaattaat
    10561 ctgaggaaaa ggatgccctt tttcaccatt ctacttttac tcatgttttt ctttgttcta
    10621 attccaagcc atcctcccgt cgtattattt ggcttattct ccgtttatgc tttatctggc
    10681 tattttatga aattgtggct ttttagcaaa aacaagaaaa aaagtgaaac tcaagaacag
    10741 gatgtgactt agccagtttt tgttgttgca ggaatataaa aaacatttta tatttcagca
    10801 ccatggaaaa cttaattagt aagcatttcg taaacctgtt gttctctcct aattctgtct
    10861 caagaatagc ttggggctat ctactgcgct tcgcgcgcaa ataattctaa tactttatac
    10921 ctttcttgat ttccctaaaa aaaacgattc tcgttttttt atttaatcgt agtcctgtag
    10981 gagaaaaata atgcaagatc gtttaatcat attcgacacg actttgcgcg atggagaaca
    11041 aagtcctggt gcatccatga caaaagagga aaaaattcgt atcgctcgcc aattagaaaa
    11101 aatgggggtc gatgtgattg aagcaggttt tcctgctgcc tccaatgggg actttgaagc
    11161 ggttaaggct gtcgcaagta atgtaaaaga tagtactgtc tgtggattgg cacgcgcgat
    11221 tgaatccgat attcagcgcg ctggtgaggc attaagtggt gcaagtgccg cgcgtatcca
    11281 taccttcatt gcgacttcgc ccattcatat gaagaataaa ctgcgtatgt ctccagaaca
    11341 ggtcatagaa caggcagtaa aggcgataaa gtgggcaagt gcctacactg ataacatcga
    11401 attttctcca gaagacgctg gaagatcaga aattgatttt ctttgtaggg tgttagaagc
    11461 agtcatcaat gctggtgcta aaacattaaa tattcctgat accgttggct ataccatgcc
    11521 tgagcagttt ggtcggttaa ttcgaacatt gcgtgaacgc attccaaact ctgacaaagt
    11581 catcttttcc gttcattgcc acaatgattt agggcttgca gtggcaaact ctttagcagc
    11641 agtatctaat ggtgcaagac aagttgaatg cactatcaat ggacttggtg agcgcgctgg
    11701 taatgcagca cttgaggaag ttgtcatggc cgtcaggaca cgtcaagact atttttcatg
    11761 tgatactcgt attgatacta cacatatcgt tcaggcgagc aagttagtat ccggtatcac
    11821 gggtttcccc gtacaaccga acaaggccat tgttggcgcc aatgcattcg ctcatgaatc
    11881 gggtattcat caagatggtg tccttaaaca tcgtgaaacc tacgaaatta tgaaagctga
    11941 agatgttggc tggaatgcca acaaattgct gttgggtaaa cactcagggc gtaatgcctt
    12001 tcgcacccga ttaaacgaat taggtatcga gcttgagtca gaagaaatgt taaatgctat
    12061 gttcatgcgg tttaaagaac tcgctgataa gaaacatgaa atctttgatg aagatctcca
    12121 tgccttgatg tcggatgagg ctcatattcc agaagagcga tatcgcctgt tatcattgat
    12181 ggcgcatagc gaaacaggag aacctccttt cgctcgatta gtcatggcga atggtagcga
    12241 tgaacttcat accgaatctg atggaagcgg ccccgtcgat gcaacttttc gtgctataga
    12301 aaaaattttg aaaagtggtg cggagcttca gttatattca gttaataaca ttacaagtgg
    12361 aacggatgct caaggggaag tcactgttcg gctgcaaaaa tcgggaagaa tcgtcaatgg
    12421 acacggtgca gatacggata tcgtcgtggc ttctgccaaa gcttacctca gtgcgatcaa
    12481 taagctatac agcaaactag aaagagctca tccacaggtt taataaaaat gaaacactta
    12541 ctttgctacc tatttatcgg gctctgtttt attactaatg ctatccatgc ggaagaattt
    12601 gcattccgat ttcaggatag cactggtaaa gtccataagc ttagcgatta caaaggtaag
    12661 tgggtactcg taaatttttg ggctacttgg tgtccgcctt gtttagagga gattcccgat
    12721 ttagtgacac tttatgaaaa tcgaagcgat atcatggtgt ttggggtcgc catggaatat
    12781 tctgaagtaa aaacggtaat ggaatttgca aaagcattat cggttaccta ccccatcata
    12841 ctaggtaacc gagtcattgc agctcaattc gatgagcttt caatgctgcc cagcacttat
    12901 ttattcgacc ctaaaggaaa tccagcggcg cgtaaagttg gattgctaac acgcgctgat
    12961 atcgaagctt ttattcagaa ctaccccaaa taatccagaa tgttattttc taatactttc
    13021 tgaaagtagg ttactctaaa atactggatc atgcatcgta tcatctaaaa actatcgatt
    13081 agaaagcagt gagttagctc gggtatgtta agatgttatt tattcggctt cattgggacc
    13141 tgattcgtac aatctcacgc gatacgattt ctggactaag cccaagtagg tgtgcaattg
    13201 agacaagact attatttctt ataatcacta tttcatctta accagtaata aaatttttac
    13261 tttatgttca gtaatctcct taaaaagatt tttggtagcc ggaacgaccg tttagttaag
    13321 caatatactc aggtagtacg tgctatcaat ggattagaat cgacgattgc cgcactcagt
    13381 gaccaagatc tggtcaacaa aacacaagca ttcaaagagc agattgcgaa tgggcaatcc
    13441 ctcgatgagt tgttacctga agcattcgct gtcgttcgag aagctggtaa acgtgtcgta
    13501 aaaatgcggc acttcgatgt tcagctaatt ggcggtatgg tacttcatga ttctaagata
    13561 gcagagatgc ggacaggtga aggtaaaacc ctgatggcaa ctttaccagc ctacctaaat
    13621 gcgctttcag gacaaggtgt gcatatcgta accgtaaatg attatcttgc cagacgtgac
    13681 gctgaatgga tgggacaaat ttatcgcttt ctgggcttat cggttggcgt agtcatgtca
    13741 caaatgccac atgaattaaa gcagcaagct tatgcagcag atatcactta tggaactaat
    13801 aatgaatatg gttttgatta tttacgggac aatatggttg gccacccagc cgagcgtgtt
    13861 caacgggtac tgaattttgc gatagtcgat gaagtagatt cgattttaat cgatgaagca
    13921 cgaacaccat tgatcatctc cggacaagct gaaggtgata ccgatatcta catccgaatc
    13981 aatcgcctaa tacccaaact tactcgccaa gaaaacgaaa aaagcccggg tgactatagc
    14041 gttgatgaaa aagcacagca agtactactc agtgaagctg ggtttgagca cgcagaaaaa
    14101 ttattatcag acgcaggttt gctcgccaat gatacaagtt tgtatgatcc gatcaatatc
    14161 aatttgattc atcacttgaa tgcgggattg cgtgcacacg cactatttac ccgtgatcag
    14221 cattatgtgg ttcagaatga tgaagtcatc atcgtcgatg agtttacggg gcgtctcatg
    14281 ccaggtcggc gttggtccga aggtctgcac caagccgttg aagccaaaga aaatgtttca
    14341 attcagaaag aaaatcaaac actcgcttca ataactttcc aaaactattt tcgtatgtat
    14401 caacgtttgt caggaatgac aggaacagca gatactgagg catttgaatt ccagcagatt
    14461 tatggcttag aaaccgtggt aatccctaca cataatccaa tgatacggga tgatcgcatg
    14521 gacaaagtgt accgcaccat gaaagaaaaa taccaagcgg tacttgaaga cattaaagaa
    14581 tgctatcaac gcggccagcc gacgctagtc ggtacaacct cgattgaaaa taatgagctg
    14641 ctttcttcat tacttaacaa agaaaatctt ccccatcaac tcctaaacgc caaacaacat
    14701 gctcgggaag ccgatatcgt tgcacaagcg ggtcaaccaa aaatgattac catcgcaacc
    14761 aatatggcag gacgcggtac tgatattgta ctcggtggta atcccgagcc ggaaatcaat
    14821 cgtatccgaa tggatgaaag cttagatgaa ccgacaaagg caaagcaaat cgaagaaatt
    14881 cggaaagtat ggcaggcaag acatgaagaa gtcgtcaaat taggtgggtt acatattatt
    14941 ggaactgaac gccatgaatc acgccgtatc gataatcagc ttcgtggccg ttctggacgg
    15001 caaggtgatc ctggctccag tcgattttat ctcgcactag aggatcccct cctacgaata
    15061 tttgcctcgg atcgtgtcgc aaatattatg acgcgactca aaatgcctga aggcgaagcg
    15121 attgagcatc catgggttac tcgcgcgatt gaaaatgcgc agaaaaaagt agaggcaaga
    15181 aatttcgata tccgtaagca attactcgaa ttcgatgatg tcgccaacga tcaacgtaaa
    15241 gtcatctacc agcaaagaaa tgagctccta gaggcgccgc aggatattgg tgaaaccatt
    15301 actgcagtta gagaaagtat attaacaagc ctgttcaatc gccatattcc acccgagagc
    15361 attgaggagc aatgggatat tccagggtta gaaaaagaac tagcaagtga ttatcacttg
    15421 acgttaccca tccatgagtg gatagaaaaa gaagacgatt tacaatctga agatatttta
    15481 gaaagaattt tagaaatcgc agcgcaacat taccagcaaa aatctgagct agtaggtaat
    15541 cacatcattc atcaatatga acgggcacta atgctgcata gtctcgataa tcattggcgt
    15601 gaacatttat ccgccttgga tcatttgcgg cagggtatcc atttacgtgg ctatgcgcaa
    15661 aaaaatccta agcaagaata caaacgagaa gcctttgaac tatttggaag tatgcttgaa
    15721 gcaatcaaag ctgaagtaac aaaaatattg ttaacggtcc aaatcaagaa tgagcagcaa
    15781 gttgagactg catctgaagt tcatacgcct gttaacgtgc aatatcagca tgcaacttat
    15841 gaagatgagc tcactcagga acaacaagcg acacctgaaa aagcatcaca agaaaacaaa
    15901 acaaaaccct ttgtaagaaa aatggataag gtaggtcgca atgacccttg cccctgtggt
    15961 tcaggtaaaa agtataaaca atgccatggt aaattaaagt aacctgctaa ccccacgctg
    16021 gctttaccag tatcatgata cattgcggtc ttgaatggta atacgcgtca gaagatcggt
    16081 tgtcgaaatt ccatccgtat agtcaatttc tttgatcata ctcgctggaa gttcggcaca
    16141 gagtttatat ttttcttgcc gctctttctc ggaagagcaa gcaaacgtat aaagatcata
    16201 gccctgttgc tgcataaatg caggcgttgc atagagcggc ccttcggtca caacagcatc
    16261 aacatattta caggctgaaa caactgcagc tctctcagac tgactcatta cagggtgctt
    16321 acctttattt tcctgcacac gttgatctga taccacaaaa acggttaggt gcgtacccaa
    16381 cgcttttgct tctcgcaaga aattaacgtg cccaacatga aataaatcag cgaccatccg
    16441 ggtataaact cttttctgtg acctattact cggccattca gggatagctt gatttaatcg
    16501 agttaacgca cgttctagcc actggcgact gcgcaaactg ataacgtcat ctgggtcctt
    16561 caatgcgatc tggtgcgcat aactccaagc ccttaccaat tcacccgata accatcgctg
    16621 aatcaatcga tagtaagcga agcagcgtac gagcaacgtg aatttttcta gatttactgc
    16681 tgaaactacc gtatcccaat aaggattggg ggttctccaa ttcccataaa atgcagttag
    16741 tatcgtttca ggtggattcg ggaaccacac agtaccataa ggtgtatctt tgggtattaa
    16801 atcaaatttt gggaaaatag aaacccgctg atattcatct ggatagccgg gaagtgagaa
    16861 tcctccaata attcgatttg ctttatttaa ctgtaagccg catagatcga gtgttatgcc
    16921 aatttcagaa tgaacataat cccgataatt acgataatta aaactcatgg gggttctttg
    16981 ccaccccatt ttctcgagaa gcgcacagca gtcatcccag ttttccatcc aaacggcaat
    17041 atctaaatct ttatcaaacg ctaataaatg accatggcga acaataccca acaaagtacc
    17101 cgcaaatgga aatgcacgaa taccgtttgc agcaagcgct gcacaagttt cccataataa
    17161 tcgctccgcc cggcctgata aaaaaatgtt ctccgttgat tccttaactg ttctggattt
    17221 ctgatccgca ggtacttttc ctgactttgt taatgccatt aaatgattaa gggcagtcat
    17281 atagtgataa caggctgttt ccagctccaa taactgaaaa caagcggtac ccatcatttg
    17341 tgcccaacgc gctttttgta aatcaccctc tgcaagacta aatattttag ccgcgtaagg
    17401 cagtacttca ccagaacgcc ctgctctcat catcattgca caccatagct gcgcaacatc
    17461 cgttaactct ggataagttt gttttaaggg ttcgagaatc tcacttgccc cattcaaatc
    17521 accagaacgg atcatttcct gggcctgccg caaagattgt ttaatcgctt cattcataaa
    17581 aataatcaaa tttgctgtct gatccttgta ttctaccgat tatttgatac tattaagaat
    17641 cactctcatt attatttata ttattagaga tagtgggata aaacaaactt gtttaacttt
    17701 caggagatta caacatggct atcactaaag aacctcgccc caatctaagt aattcaggca
    17761 atatatttga aatcatcgat ggaatccagt ttcttggttt tggttcgggc ggcttcgatg
    17821 ccaatgattt actcattatt ggcgatgaac tcgacaattt gctagaaggt aataatggcg
    17881 ctgatgatca cgatgagatc gaaggaggtg ctgggaatga cagattgctt ggacttaatg
    17941 gtaatgacac gcttgatggt gatgaaggca acgacgatct caatggtgga gaaggaaccg
    18001 acaaattatt cggtggatta ggcgatgata tcttaagggc aggttttgga catgaccgac
    18061 tcaccggcaa cgatggtagt gataccttcg gtttttatgc ggcaggtttc tatcaagtaa
    18121 aagatttttc gattacccaa aatgatcgct tattttttga ttcccaaacg ctaggtatca
    18181 atagccttga acaattagtc acctatatta ccaatattga acaacgcgca gatgggttta
    18241 aagtttattt tggtgaagtt gccacaatcg acattgttgg tgctaattta tcggctatca
    18301 ctgcagatat gattgtcttc aatctctaaa taaacacccc gtggcaagac cacggggaat
    18361 cgcaagttga atacagcgaa gcgattacaa agttttaatt aaaaacgcta taaaaaattt
    18421 gcattgcctc cataactatg tataatgaga atcattctca ttaatgattg tggaggactg
    18481 aaaatgtatc attcatacat gcacctacag acttcgaata acagcgatca ttcggcagcg
    18541 aatgatcttc gtaagtcaat caatagtgat gcgttgttca cgggcaataa tgaaatcgta
    18601 attcaacatc gtgaacaaca atatcgcttg cgtctaacgc gagcaggaaa gcttattttg
    18661 acaaagtaat ttagccaaat cgtaccaagg tcaagacaaa cagctaaatt ataatcacac
    18721 aaaatcaaat gtaattaggt ttctcatcgg tcgctggtaa tctcttctct cctcatttac
    18781 cagcgacttt tttagccagc cagcccaacc cctcatctgg gcaagccagc ctcttttttg
    18841 atgactttag gagcacggct atgaaattac ttaaattccc tttatcgcag accatttatt
    18901 gtgacaacag ccataattgg gttaattggc agcaaattag tcacatcact aatacaaagt
    18961 taaaaaacat tcaaaattgg atcactgttg tatctgcaaa gattttcaga agccacgcat
    19021 tacgctcctc gactccgcta acaacccggg tcattcccct tatcaaagag aaacaaatca
    19081 atccccgctt gctgatcttc gccaattata agcctttcaa taaaacagaa gggttggctg
    19141 gaaaaattat tctgtgtatc ggtggtcgca ttgcgctcta ttctgattat caaaaattag
    19201 tcgaaatagc gggtggaaat ttcatcgggt atcggggagc tgatatgcaa tcagatcagc
    19261 tcaagaaatt gcttgatcaa gccaatatgg tcatttgccc ggttgactgt attaaccatg
    19321 aagatttctt ttttgtaaaa aattactgcg caataactgg gaaaatttgc accttcttag
    19381 cgcgatctac cctgcttaac tttcaacaag gcttaaaaat attgatcaat ctatctcatc
    19441 acaacttagg aactgtagtg tcatgaaaaa taaaaaaatt gatgcagtaa gtttatttat
    19501 agcattccta ctgggtgtgt tttttatttt atcaagccaa cttgaggcaa aaacaattcg
    19561 agaatattgg attgctgcag aaaaagtaac ctggaactat gcaccatcag gaaaaaatct
    19621 aattgaacct gagggaaatc tcggcgtctg gggagaaacg ctcacctata ccaagtacca
    19681 atacgtcggc tacacagatg gtagcttcaa tgcaccgatt gctcaaccag aatgggccgg
    19741 gttactcgga ccccaaatcc gcgctgttgt cggtgatact attaaagttc atttccttaa
    19801 taaaacggat cggcccttat cgattcatcc tcatggtata ttttataaaa aagaggatga
    19861 aggcgcagat ggtggcaaag gtagcagtgt ccaacccggt gagcgctaca cgtatacctg
    19921 gatcgccgat gaagacgctg ggcctggtcc tagtgatccg tcgtccatcg tctggttata
    19981 ccattcccat gtaatggctg aagaagaaac aaacctcggt ttaatcggaa cgctgattat
    20041 tacgcgtaaa gggaaggaac gctcgcgtga tgatcctacc ccaaaagatg ttactcaaga
    20101 atttatcgca ctttatacca tctttgatga agaaaatggt gaagagcctg ggttaaaaca
    20161 tgctattaac ggacgaatct ttggtaatct agcgggctat caaactcaac tgggtagtcg
    20221 ggttcgctgg tatcttgtag cattgggtaa tgaagtagat aatcataccg tccattggca
    20281 tggccaaacc gtattacatc gtgggcaccg tactgacgtc atcgagctaa tgccagcaag
    20341 tatgacaacg gtcgatatgg tgcctcgttc agctggaaac tggttgtttc attgccatgt
    20401 caatgatcat atgatggctg gaatgagtac ctactggcat gtaaaatagt aactaaaatc
    20461 gacaacatca cattttcagc agcaaaaata gcattctgtt aagattcgaa tattgatgct
    20521 tgaactttcg agcatcaata ctatcttagt atcactgcaa ttatctatcg ggggcgatcc
    20581 ggtttcgacg tgggttgcaa agcagcgcag ggcataccga ggatcagtta cctcgtaaat
    20641 acatctgaaa aaaaatagtc gcaaacgacg aaaacttagc tttagccgct taatccggct
    20701 aaactccaca ccagtgggcc tcaacgctgg gtctggcaac agactgtgga gtcattaacg
    20761 aggatcgtgc tttgtagggt tactttacag agtattaaac gataggtaac tcgcttttta
    20821 tcagcctgcc gattggcgga taaggagtta aatcaaataa ttaggctaag tatgtagaac
    20881 tgtctgtaga gggttcgcgg acgcgggttc aattcccgcc gcctccacca ataccaaaga
    20941 ccaacccttc atccggttgg ttttttttca tctactcccg cgtgtttacg gggactcatg
    21001 cggattcttg cggacttcgg ggattcagga aatcccacac ttgctctttc tctctctgat
    21061 cttgcaattg ttgaaccaca gtacactttt ttataaaatc tcactttatc ttaacttcta
    21121 ccaacttaca ctcagctaaa gataaccgct tcaccgcttg tataagtgcc aatacgcttt
    21181 ctttaaaaga ttcatcaatt gttatcacag catttctagc tatatgtatt tcatcggagt
    21241 cattcgttct cgttccatta tatttccatt tcatctcttt attcacgaaa agcaagactc
    21301 cccaaagcgc agcgcacttc atccttttct acatcggtaa caaagtagat aacgctccct
    21361 aaaaaaatgg acaggaatac aatcagacaa aaaagtgtca acttctgctg catcggttct
    21421 gatggttaat taatcacaat aatagctatc aagtagacaa agctgcttta aatattttta
    21481 acgctcggtc ccgcgcaacc gcatgatcaa cgataggttt tggatactgt ggtgttccta
    21541 attctggaac ccactttcga acatagcgct ggtcagggtc aaacttttct gcctggagca
    21601 taggattaaa aactcgaaaa tagggaactg catcacaacc tgaaccgacc gtccactgcc
    21661 atcccccatt atttgccgcc aagtcaaaat ccagtagttt tttagcaaaa taggcttcac
    21721 cccagcgcca atcgatcaat aaatgtttga ccagaaagct tgctgtaatc atgcgcgccc
    21781 ggttatgcat aaatcctgtg gcattgagtt ctcgcatccc tgcatcgaca attgcataac
    21841 cggtttgacc ctcacaccag gcagaaaact cggttaaatc agtccgccaa ggaatctgat
    21901 cataggctgg tttgaatgat tttgttgccg catgcggaaa atgccataga atcatgtgat
    21961 aaaaatcccg ccaaattaac tcattgagcc atttttcatt gatggtcata gctacttgta
    22021 ccaacatacg aatactcacc gttccaaaac gtaagtggac gcttaaacgg ctcgtgctct
    22081 cgatagccgg aaaatcgcgt tgtagatggt atttttcaat tactgatcgt ttaatcgttc
    22141 gctcaggaaa aacaattccc gactttccga agcccaagtc agataacgtt ggaaatttca
    22201 agggggctat tttcttgaaa tgatcaaata ggtgcctagt atcataattt tcaatatgag
    22261 cgggtgtgag ggtcgctttc cacttccgac tgtaaggggt ataaaccgtg taaggtgagc
    22321 tatcagcttt gatgatttca tcgcgatcaa agattacctg gtctttatag gtcttgaaag
    22381 cgatttctct cttggccagg atttgttgca cttcgttatc ccgctgccga gcataaggtt
    22441 cataatcgtg attggcataa accgatcgcg gattaacccg cttaaaaaac tcggttggat
    22501 tgccatgata aacaaataaa gaagtgccta atttttctag tgccgctttc aaaattagga
    22561 gtgactgcaa aataaattca acgcgagcat ctttcttatc ttctaacctt tcaagaatca
    22621 cggtatcaaa aataaagagc gctaatacgg ttgaattttc attgagcgca tgaaacaaac
    22681 ctgcgttatc ttcaagacgc aagtctctcc gcaaccagaa aagtgttgta gatgaatcaa
    22741 tgattttctc gggagcttct ccgatgaaac gttgcaatgt atttccttta atatcaaatc
    22801 atcgcaatac cgggtgagct gagttttgcc aagcaaaatt gcgataacga ttacttcatg
    22861 ttattaatgg tttcattcct ttatttacgg aatgcagtga atctgctgat tttaagcgta
    22921 gcacaatgtc gcatactatt accaattcca accgtactga tttttttcaa actggtgaaa
    22981 cgactaagca tctggttctt aatcttttgt agcggactcg cactcctcgc gagcatcctt
    23041 tatttttctc gctatgcctt actcgaagcg ctgttaattc atcagttaca agccaaaaat
    23101 gttccgctgc agtcattcac aattcgcaat atttctctca atcaagtatt gatacgagat
    23161 ttatcactcg gcacccatga agaacttcgg atcgatcaga ttgatattaa ttggagcctc
    23221 attgatcttc tcaaaggaaa actcgatgtt gtttcattaa agggcattac catttggctt
    23281 gatttcagtg gtcagcaacc cgtgctaggg tcacttcatc ctctgcttga ttcccccagt
    23341 aacgataaaa acggccctgt aactatacaa cttcctgcaa tcgcaaaact gcaagcggat
    23401 attgattttc gctcggctca aggaaattta cgccttaact tatttggcaa gattaatcaa
    23461 cgcgaggccc taactcagtc agccgtcatc gaatttggat tgagtggttt ttctataaaa
    23521 acaaacgggc aattcaacgt tactatcgat tcaactcaaa caatccaagg tcttattgcc
    23581 atcgctgacg gttcgatcaa tcggccagat ttggagattg ctaaaattag cggagaaact
    23641 acgttcgcat tattagcgat gcagcttcgt cagttcaaag ctgagctaaa tttagatgag
    23701 attttgttcc ctgaaagtca gcaatcagtc gtgcaagcat ataagcaagc atcgcttgat
    23761 ctccaactta tagaaaacca tattcagctc actggcgcat tattaactac taataatgcg
    23821 ccagtgctag atattcaaat aaccgccaac aaaccgcaag atagtaatga tttcgatatc
    23881 gccatgaatg cgagtgggcg actaagctac ctaccttgga atctaggggg tagggagcaa
    23941 ccaagctcag ggacattttc cttgggatta cagggatcag gtcagattcc ttccaatgta
    24001 tctcagtccg gtgattcaag ctggttagaa aaaattcagt ttgagggcaa tgcacagctt
    24061 gattgtcaag ctttaagcta tgaaacgaag ctattgaatc tcagcggcag cctgggtttt
    24121 aaagtcgcaa taaataaggg aatgggtcaa gccaatttaa caccggatag cattattcaa
    24181 ttaggcagtg cagacccagc ttggttaact caatccggct taccgcctgc tttgagtaag
    24241 gatttagcga aaggcggaac gctgcgattt aatcaaacca aacaagatca actttcccag
    24301 gtcaactgga aactacaatc taaacaggtt gatattaggc ctcagctttt tgcctcactt
    24361 cgttttgcaa aaacacgtgc agattttatg ggccatggac aagttgaact caagctgaat
    24421 gaatcaaaag cagcgcacgg aataatcgat tcttggaatg gcaattttaa cttatccacc
    24481 caaatcgatc atatggattt gggatcactg accgtccata aaatgctcat gaatattccg
    24541 atgagaacct cagcttgtgg taaaaactgg agtgttgcct taaccgaacc gggtcagatt
    24601 cgattcggtc ttagcgataa aactaaacaa ttctctatca agcaaccact gcatttagtc
    24661 atttctcaac tagacattga tgcgaaacag cgtatcgatg acttttcgat aaaacaccag
    24721 gttagcgcca ccctttcgcc attttcagcc tggatgatgt cgcaaataga gccgattgaa
    24781 ataaaaatcc acccaggaag atttgcttta agtgggcaac tggataaaga ccagccctat
    24841 caggctgaag gaaaatggac tggcgctacg gcactcctcc ctcaatctca gatcaaatta
    24901 gaaaatattg tggcttccct ttcgcttggc accaagaaac gcgctcttgc gcagctcgcg
    24961 attggcaagc tatatcacct tgcagatgct ccattctttg caccacatac cctctcagcg
    25021 actattaacg ataaaacgaa gggaaagcaa cacatttata cacttgatgt atcgggtggt
    25081 ttaccaaatc taaaatattt tcacctcaaa gcagaacaga atctggacaa tggctttggt
    25141 aagcttaaat tcaatttaac tcccattcac tttatacctg ggggcactca gttatcagat
    25201 cttcttccag ggcttggaca aataaccgat atgcaaggcg tggttcacgc ccatactcag
    25261 gtcgattggt ctaaaaaagg agttcagaaa agcggtggtg ctattgtcgc agacaatcta
    25321 tcgtttaccc atcctacttt tagcatagca ggactgagaa ctcgcattga tttcgaccag
    25381 ctattaccga tcaacacacc gcctcagcag caaatccata tcaaaaccat cgatgctggt
    25441 attccattaa gtgatttgaa cattatttat caaatcgaaa gcaaccgtaa taaccagcct
    25501 cggataaagc ttgaagatac ccagttagct attttggacg gaattttaac gattgaaccg
    25561 gtaacaatcg atcccctctc aagggatacc aaaattacca ttaccttgaa taatatcaat
    25621 ctcaagcgtt tttttgactt tattcagatt gaagggctag cgggagaggg tcgcttggat
    25681 ggacgagttc ctattctttt gacggacagt cgaatcacca tcgataaaag tcgtctgatg
    25741 gctaaatctc caggaatatt gcgattccag tctgaaaagg cctcacagat gcttgcaaat
    25801 gccggtacag aaatgaatct tgtcctcgat gtgttagagg atttccatta ttccgaatta
    25861 tcgttatcca ttgataaatc agcagaacat gatctaacag ctacgctttc attactcggt
    25921 caaaatccag cagtcaaaga agggcaaatg atacgactca acattaattt ggcgagtaat
    25981 ttagacaaaa ttctcaaggc aattagttta ggttataacg tttccaatga gattctaaaa
    26041 gatttgttca gataagatta agtttgaacg acctacaata aaaacgatga gtctgatatg
    26101 ctcacggcaa ttcgagtgaa atgcggctct aagcataact ttattaaagg agtgcaatca
    26161 tgcacaacat aatacgattg ggagatcaag tcttagcggt tgcgtcgctg tctttgtttg
    26221 taatgatgat cgtggcgtgt acaccgaccg tgaaggtcga gtcgccacaa gaaccgatta
    26281 cgattaacct caatatcaag cttgatgccg atattcgggt aaagctcgaa gaagaagcca
    26341 aaaaagacat tgcagccaat cccgatgttt tctaaacata ttgcgcttga taaggagaat
    26401 gatatgcccc cattacttaa aaagaccaca aaattaaact tgctaggaat aatcaacagc
    26461 atcctattat taggattact ctatgcgttc ccagcttggt cagataatct ggagcagctt
    26521 cgcgcatccg gaatgatcgg agaaaacgcg ggtggtttcg ttgtagcacg tgatcccagt
    26581 attcaaggag aagtagaggc aatcaatgcc aaacggaaag cgatttatca agaaaaagct
    26641 acggcacagg gtgttagccc tgaacaagtt ggaaaagttt atgcatcaga aattttcaaa
    26701 aaagttccag caggaacttg gattcaattg aatgggcaat gggtaaaaaa ataaccgctt
    26761 acgttcggct caggttgttc tggaattcat gccgttaatt cattcaatta cccaataatt
    26821 cagtttactc aataaaacaa gcatgatacg tgagaacttg attaaggctt tgatagcaat
    26881 tggtgtgttt gtcgttctca tggcagcact cggcttttta tttgaagacg agctagctac
    26941 tgccacaagc tgggttgtag aacgtattgg cttcgctggt ctgtgcatga tcttgcttgt
    27001 taccgatacg tttgtaacgc cgtttcctcc cgatatcttg ctcattgtaa tcgcgaagag
    27061 ctcactttcc gaacaatggc ctttttatgt tctgatactg agtatggttt cagtttttgc
    27121 cggcatgcta ggatggggaa ttggtcgttg gctaggccat tttcaatttg cgaagcgctt
    27181 atttggcgaa ttcaaagacg agcatcgcga atttattcga cgatatggtt tttgggcagt
    27241 tgtgatcggt gctatcacac cgctaccctt ctcggtaaca tgctgggcat ccggtgtaat
    27301 gggtttacgc gcagtaaccg ttttctcagc agcagcctta ttccggatac cccgtttctt
    27361 tatttattac tggataatcg cgtcaacaag tcagtggttc taatcaacgt aaatacattt
    27421 cgttaactaa ataacgactg tattgaaatg gggcagttta attgccttat agttgaaggc
    27481 tttgatttgg taatacgtcg gttcaaagat tttatatttg acaatatctg caggcgaaac
    27541 ctcgctgaca ttgatatctt ccggaaaaat accagcagcc tttaaacccg cagcaactag
    27601 ttctgaacaa aacatttgcg ataaattttc ttcgcttcgc gtaatgccga tccgatccag
    27661 aaaatcaaaa cccgctttcg gcatattttt ataatcatag cctttaccga cttgctcctg
    27721 taagaacgct ttcatttttc caatatcagc ttttcttcga atttcagctg acaaacgggc
    27781 aatccaaata tcccctttat aaaatggaat ggaattcgaa aattgactaa taatgacgcc
    27841 ctctcgctcg ggatacatat caccctcaga agcttcaata aatgagatcc gatcattctc
    27901 acgaatgatc gacgcaacat gggaagcagg aaatcgggtc accaaacgga taattttcga
    27961 catccggtat tgccccccga agacgacgat atccccagta tcaagctcag cacgtatgtt
    28021 tttataactt acggccactg gatattcttt aaattgatcc cgaatcatta ttcccccctt
    28081 ttttgttagg ctaataacac tatcagcatg actggaaagt atcaaagatg aaatacaaag
    28141 tcaaaatcaa acccttattt gcgtcagggc aatcaaataa ttgatcggta ttagttataa
    28201 atgttggtca catcattatt gttttaataa tgcaatactt agcttcctga gatcctgtat
    28261 gagtttgttg tgaatcagtg acaaaattat ctcttctctt atcaagctgt tactgaattt
    28321 cttatcgatg gtatgtagcc tagaagcgtc aaatcactat tacttatttc agtcaattta
    28381 tcaaataatc actcaactaa ttgtttctaa tagaaaataa aatactggca tagttactgc
    28441 taaggttatt ttactcaaat aacttgagtg ctggtgcaat ggtactgcac tgagctactt
    28501 tctttaacgc tcgcatagca attttggcga gcatttcatc taggagtatt tcagttatga
    28561 ctcaagcaaa tgcttatgaa caatacatgc tcgaactcat caatattgaa cgtgctaagg
    28621 tcggctcaca accgcttacc tttgatggcg atttaaatga atcggctgag aaccatagta
    28681 cctggatgat cgctacagat actttctcac acacgggtgt cagcggttcc agtcctggag
    28741 accgtatgaa aaccgctggt tatatttttt ctggatcttg gagctgggga gaaaatattg
    28801 cttggatgag cacacgcgca ccaactggcc ttcaggacga ggtgctacaa ctccatacca
    28861 tgctgatgaa ttcttcaggc catcgggcta atatcctgaa taataatttt cgtgaaatag
    28921 gggtaggatt cgaagtaggc cagtatcagg cttatgaagg cgcatttgta actcagaatt
    28981 ttgccaagac gaataccaat ccattcttga caggcgtagc attcgacgat cgggatgatg
    29041 acttgcgcta cgatatgggc gaaggattaa gcaattttac cgtcagcgca aaaaataaca
    29101 ctaccggtgc gattaccaca accaaaacca cactcgctgg aggatatgaa ttggaattgg
    29161 ctgccggtaa ttacacgatc agcttttccg cacccggctt tacgacaaca acgaaacagg
    29221 catcgattag cactaagaat gttaaactcg atcttatcga tcccgttggc agcaacacca
    29281 tcaacgggac atctggtccg gatacattac aaggcactac aaatagcgat attatctttg
    29341 gtagtggggg taacgatatc ataaacggta acgcaggaaa tgatcgaatt gatggaggaa
    29401 ctggtaatga tcggataatc ggtgcaaatg gatcagatac gctaactggt ggttctggac
    29461 aagatacttt tgtgttcaat acgccgattt cgggaggaat cgatagaatt agcgacttct
    29521 cctcgctaga cgacgtaata catctagcgc gtagtacatt ctcgggatta tctactggcc
    29581 aattaccgat tgctgcattt catattggaa cgggcgcaca tgatagtaca gatcgtataa
    29641 tttacaattc atcaacaggc gcacttcttt acgatgcgga tggcctgggt ggaacaggct
    29701 ctcagctgtt tgcacagctt tcacccggcg tcatagttac caacactgat ttttatatca
    29761 tctaaggcgt agtccctcag cttaggcaat atgttttact aaactggggg aaaaaattga
    29821 attctattta tgaaataact cgacaggcct acattgatga ttaccaatta cgaacttgcc
    29881 cactatcaag atgaataaaa ttagaacctg gataataacc aacaccaccc acctgcattg
    29941 acaatgccgc cttcctgaca tcagaaagcg agaattctgg caagcggata tcgattgctt
    30001 taccttgcat atgtaggcta tgttttgcca caccatatcg ctgtgcagct aacaaccggt
    30061 tggtctcagg cgatcgataa cctgaaacaa cctgaaactc tttctttgca cccaacaagc
    30121 ggtgcaaaag ttgaaccata tcaagtaaac gaacatcgat cgcatgccgc tttccagtgc
    30181 gatgatctct tagtacgtta tcgatttctc taagtgcacc gttaatatat cgtccttttt
    30241 cccagtaaac tgctttaact ttctctccag tatgcaggtt atgaaatgaa atctgcctta
    30301 tacctgtctt tttcgtataa tttgcataag ctgcttgggg caacaccaat ccaatacaag
    30361 cccctaaacc cgctttaata aagaaccttc gattaatacc agaattgatt ggttgttcat
    30421 cgtcattaaa aggcttctct aaaagcatct ttaaccctcc agtctaaaat caaaacttat
    30481 agccaaaaac taccagcgag cgtactctaa tagccgctta tcacgtccat aaacatcttc
    30541 agcgaaatga atatgatctt ggtcgtctac ccaagctgtt aaataagtta ggtaaattgg
    30601 cacacgtttt gttaaattga tggtgattgt ctcaccactg ttaatttttt cagttagatt
    30661 aattccagaa ttatttccat ttaaagcaaa tcttgccagt tcccaaggct tctctaagcg
    30721 aatgcaccct gagctaaatg ttctaacatc tttttgaaac aattgctttg aaggcgtatc
    30781 atgtaaatat atgctaaaag gattcgaaaa taagaacttt atatatccta atgcattttt
    30841 cttgccagga ttttgtctta aaacataggg aaatccccct tttatcgaat tccaattaat
    30901 tgaatccgga ttaattggca cattttcatg tctggaaaaa acttttattc cttgagaagc
    30961 aaagtatgca gaattttgct gttgcttggg tagcaagtct tttacagcaa tactctgagg
    31021 tacattccag tagggattga gtaccatttg cgatagctgg ctatcgaaac ttggagtcga
    31081 tcgataatct cgacctacaa taattcgcat ttccaatacg tgcttgcctc cttcaattgc
    31141 tgacaaataa aaccctgcaa tgttgatcat cacatgccgc tcacctaagt ttcttggcat
    31201 ccaacgcaag cgctccatat tcgctcgtat ttgttgaatc ctttgcttag gggtcctatt
    31261 caacgcaatg atagtttgtt tcccaatgat tccatcggga ttcaagccat gttgctcttg
    31321 aaactgcttt actgcttcaa ctagcccgga atcataccgt tcattatcaa ttttgacaat
    31381 accatactta gggtcattct gaatattata gagccgcagg atacgcttgc gaacgagtgg
    31441 tatgctctta tgcaaagtat ttggacgaat agccgccgat tctggaattg actgccaatt
    31501 ctgctgttga acctcaagat ctcgatattt tgccaatgca gtttttaata attgatagtg
    31561 caagctctga ggaggtagct caactaagga tcgtgctaaa tgattttcgc gaattgcatc
    31621 attgagataa tctacaggat caaatcggtg ttgctcgata tgccagtctg gatcggcctt
    31681 tgaggcgagc aaacgaccac ttctcaaatc ctttgccagc attaataaag actgtatcgc
    31741 tgttaattct aattcgagtg agggtgaatc aacgccatct cgcttttgta attgaataag
    31801 cttatttaac tcataatctt gattatccaa tccatgctgt tcagcgctac cgataaagga
    31861 aatcacttta tcaatactga ttccagacgt ggagcttgat ccccaccaaa tcggctgata
    31921 atctcgtttt acatagaaac gtttcaacat agtcaaactc gtctcattgg ctgtagtaac
    31981 ggggatatgc gctaattttt cctgcaatac gaatgcattt gcttgggcaa aatgattacc
    32041 cgttgtaact agcaataaca ccaaaatact gattaagcac aacagtatct tataagtaaa
    32101 tgcaatgcta aaacgtaatt gctggaaact tgatatccgt tccatatctt ctggctataa
    32161 aaaggtagaa tctttaagat tccaaatcac aacccaatgc gatcacattg attcaaaatc
    32221 gcacacaact accgctgatg ttggataaat caagacttat tcgaagtttc agcctgatca
    32281 agccaggttt ctaatccaag cgtattgagc ttgatgtcaa ctttcaaaag atcaaatatc
    32341 gcatcagggc gcaatttaca accatgcgct tgtaagcgtc gctctagtag cgcttgcatc
    32401 gcttgcacca tatagagatg tctttcattt tgggtaactg cacgatttgc aatatgcaca
    32461 aattcatcaa ttagcgcatg catttgttgc gcatgatgcg ataaattgcc aattcggctg
    32521 taatgtgcca aataagcaaa ttgaggatgg taactcatga tgcgatcgat ggatgcatga
    32581 gcggcatcag gatcaaattg aacgggtgtc gtagtaggaa atacaaattc tagtccatca
    32641 acatccaatt ctcggtagga aaccccaaag gtatcaccag taaaaaatga ctggctctgt
    32701 gcatcaaaga tgcaataatg atggcgcgca tgaccaggag tgtctagcaa caacaattgt
    32761 ctaccattta gctcaataca agtctcatca ggcgcttcca ctacccgatc tgcatcgatc
    32821 ggatatatct ctccatagag tcgcgcaaat tctgcttcac cgtaaactgc tttgacaccc
    32881 gcaatcaatt tggcaggatt tatcatatat ttcgctccac gcgggtggac tactaattgc
    32941 gtattaggaa gcaatcgcat caattcgctt gctccacctg catgatccaa atggatatgc
    33001 gttagaaaaa cataggctac gttttcgagt gaaatctgct tgtgctccag tactgcgata
    33061 acaccaggaa ttgaaaattt tgttccggta tcgacgattg ctgcgacacc cttctcaacg
    33121 atcaaatgaa tcgctgcgcg tcccggtcgg tgatattggg catcaatcgc actgatcgca
    33181 tgaggaaaat caatagtatt tggaaaattc atagaattat tattaaaaat ctttatgtaa
    33241 ccaattcaca agaaaattgg tcaattgatt gattgcttta attggtcaag aatagctgga
    33301 ttttctagcg tcgaagtgtc ttgcgtaatt tcttcgcctt ttgcaaggga acgaagcaat
    33361 cgccgcatta tttttcctga tcgtgtctta ggaagattat caccaaaccg aatttcttct
    33421 ggttttgcaa tggggccgat ttcctttgct acccagtcac gaaggatatg ggcgatttct
    33481 tgagcttgct caccttgcgg tgacttttct ttaagcacaa caaaggctac aacagcttct
    33541 cctttgatct catgcggctt accaacaacg gctgcttcag caactaatgg attagccacg
    33601 agcgcggatt caatttccat ggtccctaag cgatgtcccg atacattcaa tacatcatca
    33661 attctcccca taatccagaa atatccgtcc ttatcacgat gtgctgagtc acccgctaaa
    33721 taatagcgcc catcaccaat atcatgagga aaatacgttt tcttaaaacg cgctggatca
    33781 ccccataggg ttcgaatttg agaaggaaag ggacgtttga tgactaagaa tcctcctttg
    33841 ccttgctcaa catcatgacc cgcttcgtca acaatcgcta cactgatgcc cggtagagga
    33901 aatgtacatg accctggttt gagcggcata gcgcctggtg taggggcaat catgtggcag
    33961 cctgtttccg tctgccacca agtatcgacg atggggcaac gtgattgacc tacaacagtg
    34021 taataccaca tccacgcttc cggattgata ggctctccca ctgaaccaag caaccgcagg
    34081 gaagacaagt catattgttt tggtaaatct acccccagct tgatcaaaga tcgaattgct
    34141 gtcggggccg tataaaagat tgttacttga tgattttgaa taatacgcca gaaacgcgct
    34201 gcatcagggt aagtgggaat accttcgaat attacctgtg ttgcgcccac agcgagcggg
    34261 ccatacgcaa catagctatg cccggtaatc caccctacat cggctgtaca ccagaaaata
    34321 tctgaaggct tataatcaaa aatccactgc atgctgacaa tggttccaag caaataacct
    34381 gctgaggaat gttgcacgcc tttgggttta ccggtagatc ctgatgtgta gagcgtaaac
    34441 aatggatgtt ctgcattcac ccattccgga tcacaatcga gcgccttatc ttggatcaac
    34501 tcatgccacc aaatatcacg accattttgc catggaacgc tagtaccggt atgtttatac
    34561 acaatgatag attgaacgga ttccgtattg cccatactga tcgcttcatc aacggtcaat
    34621 tttagtgggt gttgcttacc acctcgcgtt tgctcatcgg cagtaataac cgcaatcgct
    34681 cccgcatcaa cgattctttc gtgaaggctt ttagcagaaa acccgccaaa cactactgag
    34741 tggatcgcac caatccgtgc acaagcttgc atcgcaacga ccgcctcaat gcgcattggc
    34801 atataaatga tgactcggtc acctttcttg atattcaatg ctttaagtcc attcgcaaat
    34861 tgacagactt gatgataaag ctcacgatag gtgcaatgag ttacttttcc atcatccgct
    34921 tcaaatcgaa tggctacttt atcaggttgg tctcttaaat ggcgatccag gcagttataa
    34981 gaaatattta attcaccgtc ctcaaaccat ttaaaaaaag gagcatctga atcattgagt
    35041 attttggtaa aaggcttgtg ccacataata tgttgcttgg ccagctcact ccagaaaccc
    35101 agataatcat tttctgcttc agcacataac gcgcgatagg actcattccc cgaaatattc
    35161 gcttgctgca caaattcaac cgaaggcggg aaaatgcgcg tttcgtgtaa cacggattcg
    35221 attgcagcca taaaataatc cttttgatcc tgttttaatt aaatatccat tggagtaatt
    35281 gtaacattca catccttgga atgaagtagg cgtaatttcg caaaagctga tgatcgaatc
    35341 cagacaagtt atttcttgtt taagcgagat aggataatat tcttgttctt ttagacgatt
    35401 gaaaaatgaa gaccttatac gacaaattat gggaagctca cgtagtacat gcagaatcgg
    35461 ataaacccga tagcactgtg cttttgtata tcgatcgcca tttagtacat gaagttacta
    35521 gccctcaggc attcgaagga ttgaaattgg ccgggagaaa accttggaga acccgttcca
    35581 ttttagcagt cgctgaccac aatgttccta ccaccaatag aaaaaatggt atcagtgacc
    35641 ccacctcgca attgcaaatc gaaaccctag atcaaaattg tgatgagtta ggaatcaccg
    35701 aatttaaaat gaatgatcta cgtcaaggaa ttgttcatgt gattggccca gagcaaggtg
    35761 caacgctacc cggaatgacc gtggtgtgcg gtgattccca caccagcact catggtgcat
    35821 ttgcatgctt ggcttttggg atcggtacct cagaggtgga acatgtactc gccacacaat
    35881 gcttggtagc taaaaagtct aaatcaatgc aagtattagt agaaggagtg ctaggtaaag
    35941 gcgtaactgc caaagatatt gctttaaaca ttattggcaa gattggaaca gccggtggaa
    36001 ccggctacgc aattgaattt gctggaaatg cgatccgatc actctccatg gaaggaagga
    36061 tgacactctg caacatggct atcgaagctg gcgcacgcgc tggaatgatt gctgtcgatg
    36121 atactacgat cgattatatt aaaggtcgac cattcgcacc caaaggccaa atgtgggaaa
    36181 gtgcggttaa ctattggaga acattaaaaa gtgatgaggg tgcagttttt gattgtgtcg
    36241 taacagttaa tgccgctgaa atcagcccac aagttacctg gggaacttct cctgaaatgg
    36301 ttacgtcgat cgaaggctcc gttcctgatc ccgaatcgat tttcgatctc agcaaacgta
    36361 atgatgtaga gagtgcgctg aaatatatgg gcatcaaacc taatacaccg atccaagcga
    36421 tttatctcga taaaattttt attgggtcgt gcactaactc acgtatcgaa gatttgcgag
    36481 aagctgcatc aattctaaaa ggtaagcgta ttgcggacaa cattaaattg gcaatcgttg
    36541 ttcctggctc tggtttagtg aaatcccagg ctgaacaaga aggccttgat caaatctttc
    36601 gcgctgccgg atttgaatgg cgcgagcctg gctgctcaat gtgtttagcc atgaatgatg
    36661 ataagcttct gccaggggaa cgctgtgctt ccacgtcaaa tcgtaacttc gaaggtagac
    36721 aagggcctgg aggaagaacc catttggtaa gtccggcgat ggcagcagcc gccgctgtcg
    36781 ctggtcattt tgtagatgtt cgtttgatac actaatggtt aactgacaaa ccatgaaaag
    36841 gagataacaa ccatgaaact attcatactt ttatcagcat tcttgatgac gctatgccta
    36901 acgggttgca atacgttaca aggttttggt aaagacattc agcggggcgg cgaagccatc
    36961 gagagaacgg ctcaataaca taagccatga aaaaatttac tgattgtgat ggattggccg
    37021 taccgcttga tcgatcgaac gtagataccg atgcaattat tcctaagcaa ttcttaaaat
    37081 caattaagcg ctcgggtttt ggtcaaaatt tattcgacga atggcgattc ttagatacag
    37141 gagaaccagg aaaagaaaat cttcaccgaa aattaaatcc tgattttgta cttaatttcc
    37201 cacgttatca gggtgctcgc atcttagtga cgcgcgataa tttcggct
//
LOCUS       DAOQLJ010000004        35475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_366_length_35475_cov_6.278713, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000004 DAOQLJ010000000
VERSION     DAOQLJ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 35475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35475
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_366_length_35475_cov_6.278713"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /gene="rseP"
                     /locus_tag="PK522_00580"
     CDS             <1..906
                     /gene="rseP"
                     /locus_tag="PK522_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNC40257.1"
                     /translation="EGESVSTWQDARWALLTQAVDRNPVVVVETVNSHGQTALRKLDF
                     SQIHTDDLDANFLEGIGFIAHQPSMDPIIGQVIAGGVGHQAGLMEGDEILAINQKEIL
                     KWEELVREIRTNPGQLIELEVLRKGQIIDVMITPESVNENGQKVGKVGIAPKVDPSMV
                     EKYLINVSYSPIVAFYKACWKTWEMTAFTFRMLGKMLIGEVSWKNVSGPITIADYAGQ
                     SAQMGVASFLGFLALISVSLAVLNLLPIPVLDGGHLMYYLIEIIRGAPLSEKTMALGQ
                     QIGMALLFTLMIFAVYNDIYRLVAS"
     gene            909..>1228
                     /locus_tag="PK522_00585"
     CDS             909..>1228
                     /locus_tag="PK522_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNC40258.1"
                     /translation="MKFSFFLIFLGLFYASTLSAFESFIVRDIRVEGIQRTEAGTVFS
                     YLPVKVGEVLDETKATSAIKTLYTTGFFKDVKLKADNGVLIVQLQERPAIAEITINGS
                     KEFE"
     assembly_gap    1229..1328
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1329..3116
                     /gene="bamA"
                     /locus_tag="PK522_00590"
     CDS             <1329..3116
                     /gene="bamA"
                     /locus_tag="PK522_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNC40259.1"
                     /translation="KNDQYSKQKLSADLEVLRSYYLDRGYLEFSIDSTQVSITPDFKD
                     IYITINVTEGPQYTVSDIKMAGNFLVPEEELRKLIKLEAGGVFVRERLTESIKLITDR
                     LGDEGYAFANVNASPEIDKEKRETTFTFYIDPGRRVYVNRINITGNDRTRDEVIRREF
                     RQMEGAWHSTSQINRSKQRTDRLDFFTEVNVETPPVANKPDQVDININVKEKPTGAIM
                     FGAGYSDLEGIILNGSVAQNNIFGTGNFLSLSVNTGRINKVIAASWTNPYWTINGVSL
                     GVDVFRRDINTAALTNVGVFNTDTAGATVRFGIPIAENDIVSLGVGGEYTHIDLKNNS
                     PQRYKDFVEGTQDRFDDKGNLIKGTKGFGSSVTNLPLTLQWARDRRDSAIWTTSGTTH
                     RLFGEFSAPFAEMNYYKVSYEQKWFFPISRLFTVMVDGEIGFGEGYAGDPFPFFKNFF
                     AGGFNSVRGYNINTLGPQEQLLNLQGEPIANRFAVVGASKRAVGTIELLFPAPFMRDE
                     KTVRLAAFFDGGNVANSWSDFLDFNNDTGPFANISYFRFSAGLAVTWISPMGPLRVSI
                     AQPLNPRDRDPFHRGDDIQRFQFQMGQQF"
     gene            3238..3702
                     /locus_tag="PK522_00595"
     CDS             3238..3702
                     /locus_tag="PK522_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNC40260.1"
                     /translation="MKIGVVNTEKILRESAPAVRAQKKIEKEFLTRDEQLKKVAAQVR
                     EYQDQLERNNKGITDEERRVKERELASLTRQYQRTQQQMREDLNARQNEEYGLILERV
                     NNAIKIIAEKEGYDLILQLQDSVYRSQRIDITNQVIEVLIEQDATLPTQSAK"
     gene            3736..4185
                     /gene="fabZ"
                     /locus_tag="PK522_00600"
     CDS             3736..4185
                     /gene="fabZ"
                     /locus_tag="PK522_00600"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNC40261.1"
                     /translation="MSTVMDIHEILKYLPHRYPLILVDRVIDLEPNVRILAFKNVSIN
                     EPYFVGHFPHHPVMPGVLIVEALAQAAALLTLKSVKAENDKNQVYYFVGIDGVRFKKP
                     VMAGDQLMLTVEITRQIKGVWKYQAHADVDGQRVTDAELMCTARAID"
     gene            4212..4784
                     /gene="rnhB"
                     /locus_tag="PK522_00605"
     CDS             4212..4784
                     /gene="rnhB"
                     /locus_tag="PK522_00605"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNC40262.1"
                     /translation="MMYEAAWVCGVDEAGRGPLAGPVYAASVILGADCSIQGLKDSKL
                     LSEKKRQSITLLIKEHAKAWSIASASVVEIDQLNILQASLLAMKRAIEALPIMPKIAL
                     VDGKHAPRLNCEIRTIVNGDRLIPEISAASILAKTARDAEMIALHERFPVYGFNQHKG
                     YPTKAHFEAIQRHGICEIHRRSFSPCRCFN"
     gene            4917..5399
                     /locus_tag="PK522_00610"
     CDS             4917..5399
                     /locus_tag="PK522_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC40263.1"
                     /translation="MLIDKDTVVSLHFFKLTNQEGEVIDESSTPVSYLHGGYGSMFPS
                     VELALQGKEAGYDLSLLMEPEDTFGEYDSELIRVEPRNAFPDNVQKGMQFEGSANNSE
                     DQMVYTVIDVTDDTVVVDGNHPLAGIALKFECSVMDVRAATLEEISHGHVHGPHGHHH
                     "
     assembly_gap    5403..5502
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5863..10401)
                     /locus_tag="PK522_00615"
     CDS             complement(5863..10401)
                     /locus_tag="PK522_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC40264.1"
                     /translation="MFSEENEMTTGEFLIQFFPEITLAEQVLLLEQNNAEFIKPIMPT
                     NPNLVLAKVKDPASLDFTLNSLSNHSNVRFVEQNSVVKVESTSDDSYYTSGNLWGMYG
                     DLSTPANIYGSQASEAWNTGFIGTTKTVLGVIDTGIDYTHPDLFQNIWINQKEISTTL
                     RGSLTDVDSDGVISFVDLNHAANVTYVSDLNNNSRIDAGDLLADSRWEDGNDLDSNGY
                     IDDIVGWDFVNNDNNPMDDAQHGTHVAGTIGAMGGNGTGVVGVNWNIQMIPLKALGPS
                     GGTSLTTTAAIDYYTNASLAVGTGQNFVGTNNSWGGSGYSQSNYDAVVRTALAGNLFI
                     AAAGNSSLNTNTSANYPSNFNTTAAAGFDAVVSVAALTSSGALASFSNYGNVTVDLGA
                     PGQSIYSTVPGNSYAALQGTSMAAPHVAGAVALYASALPSATPQQILNALLSSVTPTS
                     SLATTTVTGGRLDIGTLMAGVTPPTGVTVAGGTYTGTTANNIMNGGAGNDILYGLAGN
                     DKLNGLAGNDILEGGAGSDTLNGGEGTDIYLFSLATDHKAAEVFDYGTTGIDEVRFTA
                     TTSGTLTLYAGDVGIEKVVMGTGVAANAVTSGTTVLNVNSSTVTNSLQIIGNAGINTI
                     TGTSYVDQLDGMDGADIYIINSAIHHSAAEIADSGTTGNDEVRFSALVPGTLTLYSGD
                     TGIERVTLTGTAALNINAATVGNGLTILGNAATNVLVGTNFVDSLNGGSGSDIYLINS
                     ASEHSQAEISDSGTSGIDEIRFATTSGNILTLYAGDVGVEQVVIGTGMNSTADTSGTI
                     SAGIDASLAVKGLSLIGNAGSNSLKGSAFNDVIFGGLGVDTLLGHEGSDIYIMESSTD
                     HSSAEINDSGNVGVDEIRFTATTTGTLTLFSGDTGIEQIVIGTGTAATAVTTGTTALN
                     VNASALLAPVMITGNAGNNILTATSGNDTLNGGSGNDVLKGGEGSDIYLISAAIDHGV
                     AEIEDNGSNGVDEIRFSASIASTLTVFSGDIGVERVVIGTGTASNAISSGTAALHVNA
                     SSAANALTIMANAGNNSITGTAFNDVIDGKAGADILDGRNGADIYLISTDAEHKLAEV
                     ADTGTSGIDEIRFASIVAGTTLTLYAGDTGIERVVIGTGIESVANTTAMTSLNVNANA
                     SSNALIIIGNAGDNLITGTAYHDQLNGGNGSDIYIVSTATHHAQAEIADTGNVGTDEL
                     RFAATIASTLTLFAGDTGIDQVVIGTGTKTSAITTGTVALNINASAVTNSLRLIGNNG
                     PNTLSGTAYADILDGRSGIDSMNGGNGADIYIISTSAEHTTAEIADSGVSGVDEIRFA
                     ATAASTLTLFAGDTGIEKVVIGVGLADTAITTGTAAIKIDAAAIGNSLTIIGNNGANT
                     LIGTAFADVFDGNNGNDILTGGAGADKFVFSSQPNSTSNKDTITDFQSGSDVLHFSLN
                     VFTGLGTNPGNLASEQFWSAANAVTAHDADDRLIYNTTSGGLYYDQDGLGGNPAIQIA
                     LLGTTAHPSLTHSDIVLI"
     gene            10874..12088
                     /locus_tag="PK522_00620"
     CDS             10874..12088
                     /locus_tag="PK522_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HNC40265.1"
                     /translation="MNGIQKYFLVLTSLMLVSNLSFASTSNCTQYSTSAIYIASTGLV
                     CLQQVKVIDQSSIQLYEASLQWLGSDNPNQFSLVHADLDTTPEQLSIPSFSAENGMLA
                     IPKVDIPRQFGTERYSVNLTLAPNSDSYLFNLDSISVYINPDHVPRVTWKPYGMLNSN
                     ERRAVDALGMSLPYARLSDAIYDFDNTAVDQWDLIDKISRDSGMQAGVYQHRETFELA
                     LAFRGTETCDFPCSLDETKEYLRDLATDTALTFGFDDAQFKHALNYGKDIVNRFPNRS
                     ITLTGHSLGGGLAQAVGAVLGLKTFAFNSAPVPDDFFDDNPVSLPESALNELIHVLAD
                     IHDPVSNTDETGKFYINSKHAAPLIQFDFDLKEITPNRLAELDKLRFNRHGITKFFEN
                     ASSLMTIYANGW"
     gene            12128..14719
                     /gene="mutS"
                     /locus_tag="PK522_00625"
     CDS             12128..14719
                     /gene="mutS"
                     /locus_tag="PK522_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNC40266.1"
                     /translation="MDIESKHTPMMQQYLRIKAQHGDKLLFYRMGDFYELFYEDAEKA
                     AKLLDITLTQRGMSAGEAVKMAGVPHHAAEHYLAKLVKLGESIAICEQVGDPATSKGP
                     VAREVTRIITPGTLTDAALLDGKSDSILVALTCHKSVLGLAWLNLAAGQFWVLETTLD
                     NLLSEIERIRPAEIIIPESFDIPTELKEYGVIRQLPPNQFDFDNAVRNLTTLFRTHDL
                     SGFGCDQLHAALQAAGALYHYLYLTQGTNLAHIAGLKIEHDSAYIRMDAVTRKNLEIS
                     ETLSGETSPTLMSLFDTCATNMGSRLLKHWLHHPLRNDAMIQNRLESVAFLKNNLKGA
                     PHFTRIKQLLRPIADIERITTRIALKSARPRDLSSLRDSLIALPEMAETIANHLNAHL
                     NQLANALIPDANLVKLLRQSLQSEPALLLREGGVIADGFDEELDELRMLHSNCGEFLL
                     QLETREKTRTGIPTLKVEYNRVHGFYIEVTHIHTDKIPADYRRRQTLKNTERYITPEL
                     QAFENKALAAKDKALAKEKQLYEALLEQLTPFITLMQQIARSIAEIDVLSTFAERAET
                     FNYVMPTLTEEASITIQDGRHPVVETQVDQYIANHLNLGASSNDKKMLIITGPNMGGK
                     STYMRQTALIVLLAHCGSFVPANCARIGPIDQIFTRIGAADDLAGGRSTFMVEMTEMA
                     NILNYATPQSLVLVDEIGRGTSTFDGLALAFAIARYLLNKSQSYTLFATHYFELTNLV
                     NESERIANVHLGVVEYQNRIVFLHTLNEGPASQSYGIHVAELAGVPKPIIREAKKILA
                     HLEREANHQNPQPDLFSAISQDETIDSLPLSHPVMSHLNTLKPDELSPREALVELYKL
                     KSLLDEQ"
     gene            14968..15882
                     /locus_tag="PK522_00630"
     CDS             14968..15882
                     /locus_tag="PK522_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40267.1"
                     /translation="MAVRITPRLVELTYEAALKAFWRKEALRKFLRASHVAETFLATW
                     APEESKRDFLDKTFQELQRTDRGKAVIFEMARYLSEQTTFPDLRNWEDSAQKITDATK
                     AVAELKAWLKTQDEEIRSEKEREEAKTKARDEQQKIQRSLTDKAKLQQRLDALHPAVG
                     TQQGGYDFQNWFYDLLDFCEIQNRRPYVSAGRQIDGSLTFDGTTYLVELKFTGSQADA
                     TDIDSLRSKVDDKADNTMGIMVSISGYSSVALRQASGRKTTLLLVDAMHLYLFLSGTP
                     SFGDIISRLRRHASQTGEAYLAVGKFGG"
     gene            16360..17040
                     /locus_tag="PK522_00635"
     CDS             16360..17040
                     /locus_tag="PK522_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HNC40268.1"
                     /translation="MIPDRKQSTLVLVCKRPKMGVSKQRLAVKLGREKTLVIAKALLD
                     CALEDAAEWQGPIVIAPADDADSEWAVSLLPEARQIEVFPQRLGNLGQRLNALDQSLR
                     CVGHTQLVYIGSDAPVLQSVDYIACQQALQHYDTVLIPASDGGVALMASNLHWPELAD
                     LPWSTSQLGKALVVRCESAGQSVALLEQRADVDEFADCVKLISNLNDDPRPARQTLLK
                     RIKSIIDP"
     gene            17047..17733
                     /locus_tag="PK522_00640"
     CDS             17047..17733
                     /locus_tag="PK522_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNC40269.1"
                     /translation="MPSLSIIIPCYRDHAQVAVLLQQLRMLSALPNEIIVVDAAGDSA
                     CQAICMQYDAQWHHSEPCRGKQLLQGAQVATGDILWFLHADTRVSPNSISSIMQAVER
                     GAIGGYFRFVFDGPHAWPMLILEPAIALRSRFGTPYGDQGIFVRRIAYFKVGGHAPWP
                     LFEEVPLVKGLRNLGHFQPLLDSIWVDSRRWQRDGWWHRTLRNRMLALKFILGASPDT
                     LAKSYHSQSS"
     gene            17745..18791
                     /locus_tag="PK522_00645"
     CDS             17745..18791
                     /locus_tag="PK522_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC40270.1"
                     /translation="MREVVEAYYGETLTCSADLQTNACCTDEGMPSHLKPLMAKIHPE
                     IANRYYGCGLIAPPLLTGLTVLDLGCGAGRDAYLLSQMVGEQGQVIGVDMTEAQLAAA
                     TQYQSYHRDVFGHAASNVRFLQGDIEHLDELNLADGSVDIIVSNCVINLALDKSAVLK
                     EAFRVLKPGGELYFSDVYSDRRIPAVLASDPILYGECLGGALYWNDFLPLAKQCGFID
                     PRLVSDRPITIGNTLLADKVGNIRFYSATYRLFKLNDLEPACEDHGQSVVYRGSIPDH
                     PHHFTLDKHHRIETGRQFPVCGNTFRMLHDTRFSDHFDFYGTFDRHFGIFPGCGMGVP
                     FDQDDSRQPSSGCC"
     gene            18830..19762
                     /locus_tag="PK522_00650"
     CDS             18830..19762
                     /locus_tag="PK522_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNC40271.1"
                     /translation="MSRSDDWYQTPQGEPRGFIESRKLEEVWFHTGTACNLACPFCLE
                     SSKPGDNRLQQIRFADVEPFIHEALTLGVQQFSFTGGEPFVNKDFVRILATALSYRPC
                     LVLTNATTPLIKRLHQLAPLLKQPHSLHFRVSLDHFDEVQHDAGRGQGMFKLALEGMR
                     HLYGMGFTVSIAHQTIANCDTATVARCFEEVLTAHGLPGNLHRIEFPEFHSPASQVSV
                     PVITPHCMNTYQTEQTRQRFMCAFSRMVVKQAERMRVYACTLVDDDPDYALADTLTDS
                     LAIPVSLKHHRCYSCFRYGASCSEYRSPASVTIP"
     gene            19759..21900
                     /locus_tag="PK522_00655"
     CDS             19759..21900
                     /locus_tag="PK522_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC40272.1"
                     /translation="MNKRIWVLLGLLTLIGLYLGFDLGRFLNLETFKTYQHELVEFYQ
                     QQPWWVISVYSISYIVMAALSLPGAGVMSLAGGALFGFWVGVPIVLLSATLGATLAFL
                     AARYLFGNWVEHYLGHRLLTINQALQRDGIFYLFSLRLIPAIPFFIINLLMGLTTLPM
                     LSFFWVSLVGMLAGTAVYVNAGTQLAAIDDPAEIFSPIILSSFLLLALFPWLAKAAIA
                     ISKRHRRYARWKKPARFDRNLIVIGAGAAGLVTAYIAAATRAKVTLIESHKMGGDCLN
                     YGCVPSKSLIRTAHFLNEVRKAQSLGINPVAVEYQFQDVMARVQRVIQTIAPHDSIER
                     YTQLGVEVIQGKARITSPWTVSVNDQILSTRTIVIATGAHSAIPDLPGLDQVRYYTSE
                     TIWSLQQPPKHLIVLGGGPIGCELAQAFARLAIPVTLVARNRLLMREDNEVAALIESA
                     LQADGVKILIQCKAIRCEVIGGEQRLFLRDQAGIETMLVFDALLCAVGREARVTGYGL
                     EELGINLLPTGTIETNDRLQTIYPNIYACGDVVGPYQFTHIAAHQAWYAAVNGLFDMI
                     KRFRVDYRVIPWSIFTDPEVAHVGLSEQEAQTRKVSYEVTRFDLKELDRAIADEAAYG
                     FIKVLTVPGKDRILGVTIVASHASDLIAEFVLAMKHGLGLNKILSTIHSYPSWSEANK
                     YVAGEWKRQHINPRLLDWIERFHTWRRGGDR"
     gene            complement(21908..25366)
                     /gene="mfd"
                     /locus_tag="PK522_00660"
     CDS             complement(21908..25366)
                     /gene="mfd"
                     /locus_tag="PK522_00660"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNC40273.1"
                     /translation="MQSKIVLPAPGQFLRYTGLTGSSDTLLLTKLIQSLAPSRAVAII
                     AANALDAQRLFEEIPFFLPEAKIYLLPDWETLPYDTFSPHYDLISERLATLYQVMNAA
                     YDILIVPATTALYRICPKAFLAAHTFFIKQGSTLDLPHFRNQMTLAGYSHVNQVLSPG
                     EYSVRGGLVDLFPMGSALPYRIDLFDDEIETIRTFDADTQRSIYPVSEIRLLPAREFP
                     LDETGRSQFRVNFREKFEGDPSRSKIYKEISKGSTPAGIEYYLPLFFEQTATIFDYLP
                     QDSLICLHQDVYTATDQFWRDTQSRYQLLRGDTDRPLLPPVDLFLTTDNFFGHLKSYA
                     RIQITSQQLEQSDEVLAQSPPSVRVDRHAANPLEKLAGFISAFNSTGGRILLIAESIG
                     RREIIYEYLQDHGLSPGICQSHAEFNSSNSPFMLCAGPIQSGFVLSAELGSDQFAFIT
                     ENELYATHMHGRRNRDSRKTTSTDTLLRDLSEIKIGDPVVHEQHGIGRYLGLVNLDFG
                     EGNSEFLALEYEGNDKLYVPVSHLHLIGRYSGVSPESAPLHKLGSGQWEKAKRRAMQQ
                     VRDTAAELLNLYAQRASRQGHTFKFKQRDYEAFAEGFGFEETTDQAAAIQAVITDLTS
                     GKPMDRLICGDVGFGKTEVALRAAFIAVADGQQVAVLVPTTLLAEQHFQNFSDRFGLI
                     ADQWPVKIAELSRFRSAKEQTLALDLLSKGQIDIIIGTHKLIQEKVKFKNLGLVIIDE
                     EHRFGVRQKEQLKKMRAEVDVLTLTATPIPRTLAMSLEGLRDFSIIATAPQRRLAIKT
                     FVHPFAEGIIREACLRELKRGGQIYFLYNEVSTIQSMFDKLTRLLPEARIHIAHGQMR
                     ESELEHVMRDFYQQRFNLLLCTTIIETGIDIPSANTIIIYRADKFGLAQLHQLRGRVG
                     RSHHQAYAYLLTPDEEALGAQAKKRLEAIQSMEELGSGFYLAMHDLEIRGAGAVLSES
                     QSGEMQEIGFNLYSTMLDSAIKSLKAGQEPDLQHPLGVATEIKLHVPALLPETYCNDI
                     HERLVLYKRMANCEDDGQLDDMQRELIDRFGLLPEPARALLDSHRLRIAAKPLGILRI
                     DASSESILIQFVPNPPIAAERIISLIQSSREYNLSGPDKLKIQTKILDVTERVKRIRK
                     VMDQLV"
     gene            complement(25397..26662)
                     /locus_tag="PK522_00665"
     CDS             complement(25397..26662)
                     /locus_tag="PK522_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNC40274.1"
                     /translation="MISPFQPGGELEHFLTFATSLAIGLLIGLERERSQAAKAGLRTF
                     ALVSMLGTLAALLSQKTNSPWLLLAGFLLVGTMTIIANLRNHEDPDPGTTTVTAILIC
                     YSLGVLVWYGDTTLAVMLAIVTTILLYFKTELHGLTQNLERRDLISMLQFAVLTFIIL
                     PILPDKNYGPYEAFNPYQIWLMMVLISGISLTGYVALRFIGHRYGAVLLGVLGGLVSS
                     TATTMVFTRRSEENINLAKLAVVVILLANLTVLIRLAVISAALSPKVLPMLLPVLGSG
                     LILGLTVTIFWWRDFTQQEELPIPETKNPTEFSTAAGFGVLYALVLFMSTWLSDIAGS
                     SGLYMVAIISGLTDVDAITLSSLRLLELGKLQISEAVTAITLAIISNIIFKLGLIFII
                     GSSLLNKRTAFGMIMTAIGLAGALFIIVN"
     gene            complement(26655..28403)
                     /gene="recJ"
                     /locus_tag="PK522_00670"
     CDS             complement(26655..28403)
                     /gene="recJ"
                     /locus_tag="PK522_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNC40275.1"
                     /translation="MPNITTRVFPQAIFDWLVQQGCSPILARIYAARGIEQPSQLETA
                     LSKLLPFEQLKNIAEMAVVLADAIADQKRLLIIADYDSDGATACALAIRALRKFGACV
                     DYLVPNRFEYGYGLTPEIVNLAATSNSEYRPDIIITVDNGISSIEGVEEANRLGIRVL
                     ITDHHLPGEHLPNAAVIVNPNQPSCPFSSKNLAGVGVIFYVMLALRAELRKRGVFNNC
                     FKEPNLASLLDLVALGTVADVVKLDWNNRVLVKHGLHRIRNGKACTGIYALLKVAKRD
                     YQRISTNELGFVIGPRLNAAGRLDDMTLGIECLITDDEGRAFEIASELDRLNRQRREI
                     EATMRDSALDQLALNIKFQQPAESVYSICLYDPNWHQGVIGLIASRVKEKFHRPIIIF
                     APGNEGEIKGSGRSIAGINLRDALDLVSKRNPGLIKKFGGHAAAAGLTIMEHDFQRFQ
                     AAFEQIAQQLLTPADLTQQIETDGDLNETELSLALAQSFAEVVWGHGFPEPTFNARFY
                     VENQRIVGEKHLKLKLRKSISNTSYDAILFFQAEPLPECIQAVFRLQANEFNGNTTLQ
                     LLIEHWSDNNNEIKND"
     gene            complement(28480..29337)
                     /locus_tag="PK522_00675"
     CDS             complement(28480..29337)
                     /locus_tag="PK522_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNC40276.1"
                     /translation="MNHSSPKISWFEHIGSLLMRQPEDREQLVALLHSAYERNLLDAD
                     ALTMIEGVMHVSEMQVRDIMIPRSHMDTIDISEKPAEFIPFVIEKGHSRFPVTDGSKD
                     QMIGILLAKDLLRYYSEQESFNVREMLRPAVFIPESKRLDILLKDFRSNRNHIAIVVD
                     EYGGVAGLVTIEDVLEQIVGDIEDEYDFDENEANIVAETNGHYRVKAITEVASFNETL
                     GAAFSDQDFDTIGGVVTNKFGRMPKNGESVIIDHYRFTVLRADSRRLHLLKVEKIDAA
                     NPEASDQTD"
     gene            complement(29418..29828)
                     /gene="ybeY"
                     /locus_tag="PK522_00680"
     CDS             complement(29418..29828)
                     /gene="ybeY"
                     /locus_tag="PK522_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNC40277.1"
                     /translation="MVQYVSKFPETPNRHHFRKWVKAALVQSAEIVIRIVDQEEGLSL
                     NQRFRGKAYPTNVLTFTYDDSSPLAGDIVLCAPVVIREAQEQHKSIDAHYAHLTIHGV
                     LHLQGYDHETDLEAVDMEQLESQLMTQLGYPDPY"
     gene            complement(29884..30861)
                     /locus_tag="PK522_00685"
     CDS             complement(29884..30861)
                     /locus_tag="PK522_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNC40278.1"
                     /translation="MKPKPVEISFSPTDNQRLANLCGALDENLKQIENALDVNIARRG
                     EHFVLKGQSNQTEIAAQVLRNFYNQSHHQLSIEQVQLGLIEAANLSSQLSQSIDPAID
                     ATSSPKLMTRRNNLHARSLRQGQYLQQIQDHDIVFGIGPAGTGKTYLAVASAVDALER
                     EMVSRIILVRPAVEAGERLGFLPGDLVQKVDPYLRPLYDALYDLMGFDKTSRQFERNT
                     IEIAPLAFMRGRTLNQSFIILDEAQNTTPEQMKMFLTRIGFGSKAVITGDITQIDLPK
                     HQKSGLIDAEQVLKQIKGIAFTHFRSEDVVRHPLVQRIVNAYEQHSEQA"
     gene            complement(30946..32271)
                     /gene="miaB"
                     /locus_tag="PK522_00690"
     CDS             complement(30946..32271)
                     /gene="miaB"
                     /locus_tag="PK522_00690"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNC40279.1"
                     /translation="MNNKLYIKTFGCQMNEYDSDKMADVLHSAKGLELTDKPEEADVI
                     LFNTCSVREKAQEKVFHDLGRVRHLKDKNPNLLIGVGGCVASQEGHEIIKRAPFVDLV
                     FGPQTLHRLPELIRKRELTGRSQVDISFPEIEKFDHLPPARTEGATAFVSIMEGCSKY
                     CSFCVVPYTRGEEVSRPLNDILVEVAHLTLRGVKEVNLLGQNVNAYRGEMDSGEIVDF
                     ALLLEFIHEIPDIERIRFTTSHPKEFTTRLIDVYAKLPKVVNHLHLPVQSGSDRVLAA
                     MKRGYSAIEYKSIIRKIRALRPNISISSDFIIGFPGETEADFEATMKLIEAVYFDESF
                     SFIYSPRPGTPAADLPDDTPQEIKLERLHRLQEKIAQHATLISHNMVGTTQRILIEGY
                     SKKSADELCGRTENNRMVNFIGDPNRIGQFIDIKITAALPHSLRGEILK"
     gene            32411..32866
                     /locus_tag="PK522_00695"
     CDS             32411..32866
                     /locus_tag="PK522_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HNC40280.1"
                     /translation="MPDSINNLTSSSQDKDGLHILRLHDSWIMRWLYLGVGITALLLG
                     ILGIFLPILPTTPFILLAAACFARSSERFHVFLLGNRITGPIIYEWCMHRSIPRRVKR
                     WVYLMITLSFGSSIVIVSAIWLKLMLVILAIVLTLFIWRIPVRDRAEAG"
     gene            complement(32863..33351)
                     /locus_tag="PK522_00700"
     CDS             complement(32863..33351)
                     /locus_tag="PK522_00700"
                     /inference="COORDINATES: protein motif:HMM:NF023125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HNC40281.1"
                     /translation="MQTSQFADLYQRQMIILEKSLTQSALSTQLIQNYLSAFYQTDSH
                     FGKMTFRIGQYCDILSRLMIGAKLTNALFITACNPHSQLQCTQINDLLQIQLHKQLTY
                     YSPWIYPGESIDPRNHWPAESSYLTLGIDLLHSIQIGIQFKQNAIVWCNVDAVPRLIL
                     LR"
     gene            33391..34107
                     /locus_tag="PK522_00705"
     CDS             33391..34107
                     /locus_tag="PK522_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HNC40282.1"
                     /translation="MKLFILIILFFFALNVIAEESSRNIVPDTMTERVRACTICHQDG
                     DHDQARSTYYPRIAGKLEGYLFNQLRHFRDGRRYHQPMALLLENMSDDYLQAIARHFS
                     QLNVPYPSPESIDLKSNEIQLVEKLIRLGDPQRNIPACSACHGDTLMGNTPYIPGLLG
                     LPSAYLTAQLSGWRSGSLIRGQVTNCMSEIAKQLSYDEIIAISRWLATQAVEGEAQKV
                     LSPDLIKRCQSIFLEGVAQE"
     gene            34104..35378
                     /locus_tag="PK522_00710"
     CDS             34104..35378
                     /locus_tag="PK522_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC40283.1"
                     /translation="MSPYYFCFTSGFARKCALGYLVFFVLFGVQINLISAAPGEQDLA
                     RGEYLTRVGNCLGCHTAKGGVPFAGGRRLSTAFGTFVTPNITPDKETGIGHWTEEDFW
                     LAMHEGKSRDGRMLYPAFPYTEFTKVSRADVKSIFQYLQSIAAVSQVNPAHEIPFPYN
                     SKPLLFGWRKLFFKQGIYEADPTQNEEWNRGAYLVQGLGHCDACHTARNEVGAKKDAA
                     VGGGQMMGANWYAPSLYSRKEASVTDWSLEAIIELLSSGVSKHANVSGPMATVVQQSL
                     QYWSPQDIRAMAVYLKSLPDKTAQENVRYPEMPESAKRYLAQGEKVYEKHCQDCHGEV
                     GQGIPGIYPALANNRSVTLPVPLNLIRAVLNGGYPPTTHLNPQPYGMPPFQQLLRDDE
                     IAQVISYIRNAWGNRAGLVTIVDVDQGRGGNH"
BASE COUNT         9702 a         7467 c         8067 g        10039 t
ORIGIN      
        1 gagggtgaat cagtatcaac ttggcaagat gctcgatggg cgttactaac tcaagctgtt
       61 gatcgtaatc cagttgtagt agtagaaaca gtaaactccc atgggcagac tgcattaaga
      121 aaactggact tcagtcaaat tcatacggat gatttagatg ctaattttct cgaaggcatt
      181 ggttttattg cccatcaacc tagtatggat cccattatcg gtcaagttat tgctggtggt
      241 gttgggcatc aagcgggatt aatggaagga gatgaaattc ttgccattaa ccagaaagag
      301 atactgaagt gggaggagtt ggtgagagag attcgtacca atcctgggca gttaattgaa
      361 ctggaagttt taagaaaagg gcaaattatc gatgtgatga ttacgcctga atcggtaaac
      421 gagaacggtc agaaagttgg aaaagtaggg atcgcaccaa aagttgatcc gagtatggtt
      481 gagaaatatc taatcaacgt gagttattct cccattgtgg cattctataa agcctgctgg
      541 aagacttggg aaatgacggc gtttactttt cgtatgttgg gtaagatgtt gattggagaa
      601 gtttcatgga agaatgtgag tggcccaatt acgatcgccg attatgcagg ccaatcagcg
      661 cagatgggcg ttgcttcttt tcttggtttt ctagctttga tcagcgtgag tttagcagtt
      721 ttgaatttgt tacccatacc agtactcgat ggtgggcact tgatgtacta tctcattgaa
      781 ataattcgtg gcgctcccct ttcagagaag accatggcac tgggccaaca gattgggatg
      841 gctttacttt ttacactgat gatttttgcg gtttataacg atatctatcg cttagtagct
      901 agttaagaat gaagtttagc tttttcttga tttttctagg tttattttac gcatctacac
      961 tgagtgcatt tgaatctttt atcgttagag atattcgtgt cgagggaata caacgtacag
     1021 aagcaggtac ggtatttagt tatttaccag tcaaagtagg tgaagtactt gatgaaacaa
     1081 aagcgacttc cgcaattaaa acactctaca caacaggttt ttttaaggat gtaaagctca
     1141 aagcggataa tggtgtattg atcgtccaat tacaagaacg tccagctatt gctgaaatta
     1201 cgatcaatgg ctccaaggaa ttcgagaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnaa aaatgaccaa tattctaaac agaagttgtc agcggatctt gaagtattgc
     1381 gatcatatta tcttgatcgg ggttatcttg aattcagcat tgactctacg caggtttcga
     1441 tcacccccga ttttaaagat atctatatta cgataaatgt tacagagggg cctcagtata
     1501 cggtatcgga catcaagatg gcgggtaact tcttggtgcc agaagaagag cttcgtaaat
     1561 taattaaatt agaagctggc ggtgtttttg ttagagagag gctaactgaa tcgattaagc
     1621 taattactga tcgcctcgga gatgaaggat atgcgtttgc gaatgtcaat gcgtcacccg
     1681 agatagataa agagaaaagg gaaaccactt tcacttttta tattgatccg gggcggcggg
     1741 tctatgtgaa tcggatcaat attactggaa atgatcgaac gcgtgatgaa gtcattcggc
     1801 gggagtttcg tcaaatggaa ggcgcatggc attcaacttc gcaaatcaat cgttccaagc
     1861 aacgtacgga tcgacttgat ttctttacag aagtcaatgt agaaacaccg cccgttgcaa
     1921 ataaacctga tcaggttgat atcaatatca atgtcaaaga gaagcccact ggagcaatca
     1981 tgtttggcgc cggctactcg gacctggagg gtattattct gaacggttct gttgcacaga
     2041 ataatatctt tggaaccggt aattttctca gcctttccgt caatacggga cgtatcaata
     2101 aagtcattgc cgcttcgtgg actaaccctt attggacgat taatggtgtg agtctcgggg
     2161 ttgatgtttt taggcgtgat atcaatacag cagcgctaac gaatgtcggt gtttttaata
     2221 ccgatacggc gggtgcaacg gtgcggtttg gtattcctat cgcagaaaac gatatcgtat
     2281 ctctcggtgt tggcggtgaa tatactcata ttgacctaaa aaataatagt ccacagcgtt
     2341 ataaagattt tgtagaagga acccaagaca gatttgatga taaaggaaat ttaatcaaag
     2401 gaacaaaagg atttgggagt tccgttacaa atttaccact aactcttcaa tgggcacgtg
     2461 acagacgcga tagcgcaatt tggacgacgt cgggtacaac gcatcggttg tttggtgagt
     2521 ttagcgcgcc atttgctgag atgaactatt acaaagtcag ctatgagcaa aaatggtttt
     2581 tcccaatttc gagattgttc actgtgatgg ttgatggtga gattggattt ggggaaggct
     2641 atgcagggga tccctttcca tttttcaaga acttctttgc gggtgggttt aactcggttc
     2701 gtggctataa catcaatacg ttaggccctc aagagcagtt gcttaattta cagggagaac
     2761 caatagcaaa tcgttttgct gtagttggtg caagtaagcg cgcagtcggg accatagaat
     2821 tactgtttcc agctcctttt atgcgagatg agaaaacagt acgcttagcg gctttttttg
     2881 atgggggtaa cgtggcgaat agttggtcag acttcttgga ttttaataat gatactgggc
     2941 cgtttgcgaa tatttcatat tttagatttt cagcaggact agctgtaact tggatatcgc
     3001 cgatgggacc gctcagagtg agcattgctc aaccgcttaa tccaagagat cgcgatccat
     3061 tccatcgcgg tgatgacata caaagattcc aatttcagat gggacaacag ttctgaggta
     3121 cgattgagcg agcgagccag caattatatt cataggattt gttacagtaa gcattatctt
     3181 ttgtttgctg catgctttct tttgttcttg tcatgctcga ttaccctagc taaagaaatg
     3241 aaaatcggcg tagtaaatac tgagaagatc ctaagagaat ctgctcccgc agtcagagca
     3301 cagaaaaaaa ttgagaagga gtttctaaca cgtgatgagc agcttaagaa agttgctgct
     3361 caagtgcgag aataccaaga tcaattggaa cgaaataaca aaggtattac agacgaagaa
     3421 agaagagtta aggaacgtga attggcaagc ctgacacgtc aatatcagcg tacacaacag
     3481 caaatgagag aagatctgaa cgcgcgtcaa aacgaagaat atggcttgat cttagaacga
     3541 gtcaataacg caataaagat catcgctgag aaagaaggct atgatttgat tctgcagtta
     3601 caagattcgg tttaccgtag tcagcgcatt gatattacca atcaagtaat tgaagtgctt
     3661 atcgagcaag acgctacact tccgactcaa tcagcaaaat agcctgctta cccaaatgga
     3721 caaaggaaaa ataatatgag tactgtgatg gacatccatg aaattctgaa atatttaccg
     3781 catcgctatc ctttgatatt agtggatcga gtgattgatc tcgaacccaa tgtaaggatc
     3841 ctggcgttta agaatgtatc cattaatgaa ccctactttg tcggtcattt tccccatcat
     3901 cccgtgatgc cgggagtatt aatcgttgaa gcattagcac aggctgctgc actgctaacc
     3961 ctgaaatccg tgaaagcgga gaatgacaag aatcaagttt attattttgt tggtattgat
     4021 ggtgttcggt ttaaaaaacc ggtgatggcg ggtgatcagt taatgctaac cgtagagata
     4081 acaaggcaaa ttaaaggagt ttggaagtat caggcgcacg ctgatgtgga tgggcaacgt
     4141 gtgacagatg cagaattaat gtgtacggct agggcgattg attagtagtt ataaaatcgc
     4201 agatgaatta aatgatgtac gaagcggcct gggtctgtgg tgttgatgaa gctggaagag
     4261 gtccactagc ggggccagtc tacgcggcga gtgttatttt aggagctgat tgctcaattc
     4321 aaggtctaaa agactctaaa ttactcagtg aaaaaaaacg ccaatcaatc accttactga
     4381 tcaaggaaca tgctaaagcg tggtcaattg cttctgcatc ggttgttgag atcgatcaac
     4441 ttaatatttt acaagcgagc ttgctcgcga tgaaacgagc gattgaggca ttacctataa
     4501 tgccaaagat tgctcttgtc gatggaaaac acgcgcctcg gttaaattgt gaaataagaa
     4561 cgattgtaaa tggagatcgc ctaattcctg agatttcagc agcatcaatc ttagcaaaga
     4621 ctgcacgtga tgctgagatg atagcgcttc atgagcgttt tccagtatat ggatttaacc
     4681 agcataaggg ctatcctact aaagctcact ttgaggcaat tcagcggcat ggcatctgtg
     4741 aaattcatcg cagaagtttt tctccctgtc gatgtttcaa ttaagctttg atttgtaggg
     4801 tgaaattaag ccatgattaa gaagaagcgg attcatataa atgaatgatg gctatttgtt
     4861 gctatattga gcctaatcat ttgaacgatt tatttaccaa ccctgatgag gaaaatatgc
     4921 ttatcgataa agataccgtg gtatcgctgc atttttttaa attgactaac caggaagggg
     4981 aagttattga tgaatcaagt acaccagtga gctatctgca tggtggttat gggagtatgt
     5041 ttccatccgt ggagttggcc ttacaaggca aagaagcggg ctatgatttg tcgttattaa
     5101 tggagcctga agatacattt ggagaatacg atagcgagtt gattcgtgta gaacctagga
     5161 atgcttttcc tgataatgtg cagaaaggaa tgcagttcga aggaagcgca aataattctg
     5221 aagatcaaat ggtttatacg gtgatcgatg taacggatga taccgttgtc gttgacggta
     5281 accatccgct agctggcatc gccttaaaat ttgagtgttc ggtcatggat gtaagagcag
     5341 caacactgga agaaatatcc catggccatg ttcatggtcc tcatggacac catcattaat
     5401 cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngttactgt ggcctagaca
     5521 aagggcatac ctggtacata ttactagcga tggtgtataa cttgaaaaga ttgcccaggc
     5581 tctttgctga acatcgaatg actacgcaaa tatagaggac agtgtgcttc ctctaccgga
     5641 aatagccggt ttagtgggac tgattacacg tttatttgtt gggaaacggc taaaaagatt
     5701 caactcaacc acaaattaac tcagaatttc tctgaaatac gaaatttgcg tcattttgca
     5761 aagatctcac cgaaatgggt atttcggcct aatcgaagaa tactggggaa aaaattccta
     5821 cgttccccag tttgctaaag ctaacaaata aaagcttatc gattaaatca acactatatc
     5881 actatgcgtc aaacttggat gtgcggttgt tccaagcaag gcaatttgta tagctggatt
     5941 accccctaaa ccatcctgat cgtaatatag accgccactg gttgtattat aaatcaacct
     6001 atcatcagca tcatgcgctg taactgcgtt tgctgctgac caaaattgtt cacttgctaa
     6061 attacctgga ttagttccca aaccagtaaa tacgttaaga ctaaagtgaa gtacatctga
     6121 gccactctga aaatcggtaa tcgtatcttt attactcgtg ctattgggtt gggagctgaa
     6181 tacaaactta tcggcccctg ctccaccagt aagaatatcg ttaccattat taccatcgaa
     6241 aacatcagca aatgcagtac caataagcgt atttgcccca ttatttccta taatggtgag
     6301 gctattgcca attgccgctg catcaatttt gatagctgcg gtaccggtgg taatagcagt
     6361 atcagctaac cctacaccaa tgaccacttt ctcaatccct gtatcaccag cgaacaaagt
     6421 tagtgtactt gcagctgttg cagcaaaacg aatttcatcc acgccggaca ctccagagtc
     6481 tgcaatttca gcagtagtat gttcagcaga agtggaaata atataaatat cagcgccgtt
     6541 acccccattc atgctatcaa tccctgatct accatcaagt atgtcagcat aggctgttcc
     6601 agaaagagta ttagggccat tgttgccaat taatctcaag gaatttgtaa ctgctgatgc
     6661 attgatattg agtgcaacgg tgccggtagt gatagcgcta gtttttgtac ctgtaccgat
     6721 aacgacctga tcaataccgg tatcaccagc aaatagtgtg agtgtactgg caatcgtagc
     6781 agcaaaacgt aactcatctg tgcccacgtt cccggtatca gctatttctg cttgtgcatg
     6841 atgagttgct gtgctgacga tataaatatc agaaccattt ccaccattca actgatcatg
     6901 ataggccgtt ccggtaatta aattatcccc ggcatttcca ataataatca atgcattgga
     6961 tgaggcattt gcattaacat ttagtgatgt cattgcggtt gtgttagcta cactctcgat
     7021 cccggtaccg attacaaccc gctctattcc tgtatcacct gcataaagtg tcaatgttgt
     7081 accagccacg atcgaggcaa aacgtatttc gtctatccca gatgtacccg tatcagcaac
     7141 ttcagctaat ttatgttcgg catctgtact gattagataa atatcagccc cattacgacc
     7201 atctagtatg tctgccccag cttttccatc gataacatca ttaaaagccg tcccagtaat
     7261 tgagttgttc cctgcatttg ccatgatcgt aagcgcattt gcagcactgc tcgcattaac
     7321 atgaagtgca gcagtacctg agcttatcgc atttgaggcg gttccagttc caatcacaac
     7381 gcgctctaca ccgatgtctc ccgagaaaac agtcaaagta cttgctatcg aagcactgaa
     7441 tcgaatttca tcgaccccat tcgaaccgtt atcctcaatc tcagcaacac cgtgatcaat
     7501 tgcagcagaa ataagataaa tatccgagcc ttcacctcct tttagaacat cgttgccact
     7561 tcccccattc agcgtatcat tacccgatgt tgctgttagt atattgttcc cagcatttcc
     7621 agtaatcatt accggggcca agagcgcgct agcattgacg tttagcgctg tggtccctgt
     7681 tgtaactgct gtagcagcag ttccggtccc aatcacaatc tgttctatgc cggtatcacc
     7741 cgaaaataaa gttagcgttc cagtagtggt cgccgtaaat cgaatttcat cgacacccac
     7801 attcccacta tcattgattt ctgctgaaga atgatcagtc gaactttcca taatatagat
     7861 atccgaccct tcatgaccga gcaaggtatc aacgcctaac ccaccgaaaa ttacatcatt
     7921 aaaagcactg cctttgagtg aattagatcc tgcattgccg atgaggctca atcctttcac
     7981 agcaaggctc gcatcaatac cagccgatat agttcctgag gtatcagccg tgctattcat
     8041 acctgttcca attacaacct gttcaacccc aacatcccca gcataaaggg ttagaatatt
     8101 accgctcgta gtagcaaatc gtatctcatc gataccactc gtgccactat cactaatttc
     8161 tgcctgacta tgctctgatg ctgagtttat caagtagatg tcggaaccac tcccaccatt
     8221 taaactgtca acaaaattag tgcctactaa aacatttgtt gctgcatttc ctaggatagt
     8281 gaggccatta ccaacagtgg ctgcattgat atttagtgcg gctgttcctg ttagcgttac
     8341 ccgttcaata cctgtatcgc ctgaatataa agttagcgtg ccagggacaa gcgctgaaaa
     8401 acgcacctca tcgttaccag tagttccact atcagcaatt tcagcagcgc tgtgatgaat
     8461 tgctgagttg attatataaa tatccgctcc atccatacca tccagctgat caacgtagct
     8521 agttcctgta atggtattta tacctgcatt tcctatgatt tggagtgagt ttgtaactgt
     8581 acttgaattg acgttgagca ctgtcgtacc tgatgttaca gcatttgccg caactccagt
     8641 tcccataacc actttctcta ttccgacatc tcccgcataa agtgtcaaag taccgctcgt
     8701 tgttgcagta aaacgcacct catcgatacc cgtcgttcca taatcaaaaa cttctgctgc
     8761 tttatggtct gttgcgagtg agaaaagata gatatccgtt ccttctccac cattaagcgt
     8821 atcggatcca gcgcctcctt caagaatatc attgcctgct aacccgttta acttgtcatt
     8881 ccctgccaat ccatagagaa tgtcattacc cgcaccaccg ttcatgatgt tattggcagt
     8941 cgttcctgta taagtcccac ctgcgacagt cacaccggta ggcggtgtaa cgccagccat
     9001 taacgttccg atatctaatc gtccccccgt aactgttgtc gtagctaaag agcttgtcgg
     9061 tgtaacagag cttagcagtg cattcagaat ttgctgaggt gtcgctgatg gcagtgcaga
     9121 tgcatacagc gcgactgccc ctgccacgtg aggtgcagcc atagaggttc cttgtaacgc
     9181 cgcatagcta ttgccaggaa ccgtagaata gatgctttga cctggagcac ctagatctac
     9241 tgttacgttg ccatagtttg agaaagatgc gagcgcaccc gaacttgtta atgctgctac
     9301 cgaaacaacc gcatcaaagc ctgctgctgc ggttgtattg aaatttgatg ggtaatttgc
     9361 agatgtgttg gtattcagac tactattgcc agccgcagca ataaagaggt taccggctaa
     9421 tgctgttctt actactgcat catagttact ctgggaataa ccacttcccc cccaagaatt
     9481 gttagtgcct acaaaattct ggcctgttcc taccgctagg ctggcattgg tataataatc
     9541 aatcgcagca gttgtcgtta aagatgttcc gccagaaggt cccagtgctt tgaggggtat
     9601 catctgaata ttccaattca cacccactac acccgttcca ttaccgccca ttgcgccgat
     9661 cgtacctgcc acatgagtgc catgctgcgc atcgtccatc ggattattat cattattaac
     9721 aaaatcccaa ccaacgatat catcgatgta gccatttgaa tcaagatcgt taccatcttc
     9781 ccaacgactg tcagctagca aatctcctgc atcaatccga ctgttattat tcaaatcact
     9841 gacataagta acattcgctg catgattcaa atcaacaaag cttataactc catcgctatc
     9901 cacatcggta agagatccac gtagcgtggt actaatctct ttttgattaa tccaaatatt
     9961 ttgaaaaaga tctggatggg tataatcaat accggtatca attacgccaa gaactgtctt
    10021 tgtggtacca atgaatccag tattccaagc ttcactcgct tggctgccat atatattggc
    10081 aggggttgag agatcaccat acattcccca gaggttacca ctggtgtagt agctatcatc
    10141 actggttgat tcaaccttaa caactgaatt ttgctctaca aatctaacat ttgaatgatt
    10201 gcttagtgaa tttaaagtga aatcaagtga ggcgggatct ttaactttgg ccaatactaa
    10261 atttggatta gttggcatga ttggttttat aaactcggcg ttgttctgtt caagtaataa
    10321 aacttgctct gctaatgtta tttctggaaa gaattgaatt aaaaactctc cagtcgtcat
    10381 ttcattttct tcactaaaca tagtttccgt acacctctcg atagaaggat ttcattttga
    10441 aaatacttca gttatgtaat gcttgtttct agataggtat gccgccaaaa ttagactgtt
    10501 ggtgactatc acaattgtga ttgttgtgat tgcttgaaaa aaaggggaaa aatatgtaac
    10561 ggtataaaaa tcgatacctt gataaccgct gcaaaatgag aagttaccgt tgataggtat
    10621 aagcatgaat gaggtgtaat caagacataa agcaatttaa atcgcagtgc ttacttgaat
    10681 tgcttgtaaa acgattaaac ccaagattct tactgagcgt atcttagatt tttttgtagc
    10741 gcatgaaaca ttactaatgc gcacctcaaa gtttagtaaa atctgactat ctataaagtc
    10801 gccaggcatg atcgcaatga ttgcatttgc cccagcttga tacctaacga ttcaatttta
    10861 tggaatgaat tttatgaatg gcattcaaaa atacttctta gtattaacga gtctaatgct
    10921 tgttagtaac ctaagttttg cttccacctc caattgcaca caatactcaa cttctgcaat
    10981 ttacattgcc tcaaccggtc tcgtctgttt acagcaagta aaggtcattg atcaaagtag
    11041 tattcaactt tatgaagctt ccctgcaatg gcttggttct gataatccca accagtttag
    11101 cttggtccac gcagatttag atactacgcc tgagcagctg agcatacctt ctttttcagc
    11161 tgagaatggc atgctggcaa taccgaaggt agatattcct aggcaatttg gtactgaacg
    11221 ctattccgtc aatttgacgc tggctcccaa tagcgattct tatctattta atcttgactc
    11281 gatatcggtt tatatcaatc ctgaccatgt gccaagagtt acttggaaac cttatggcat
    11341 gcttaattcc aatgaacggc gtgctgtcga tgcgttgggt atgtcgcttc cttacgcgcg
    11401 actctccgat gcaatttatg attttgataa tactgcggtt gatcagtggg acttgatcga
    11461 caaaattagc cgagattcag gcatgcaagc tggcgtttat caacatcgtg aaaccttcga
    11521 acttgctctc gcctttcggg gcacagaaac ctgtgatttc ccttgtagct tggatgaaac
    11581 aaaagaatat ttgcgtgatt tagcgactga tactgcgctg actttcggat ttgatgatgc
    11641 tcagttcaaa cacgccttaa attatggcaa agatattgtg aatcgctttc ccaatcgtag
    11701 tattacgcta acagggcact ctctcggtgg tggcttagca caagccgttg gtgctgtttt
    11761 aggtttaaaa acatttgcat ttaattctgc gccagttccg gatgattttt ttgatgataa
    11821 tccggtgtcg ttaccagaat cagccctcaa tgaattaatt cacgttctcg ctgatatcca
    11881 tgacccagtt tccaacaccg atgaaaccgg caagttttat attaattcaa agcatgcagc
    11941 gcctttaatt cagtttgatt tcgacttgaa agaaatcacg cctaatcgtc tcgcagaatt
    12001 agataaattg agatttaatc gacatggaat cactaaattc tttgaaaacg cgagtagttt
    12061 gatgacaatt tacgctaacg gatggtaaag attagcaatc gtcaaagatt aatacttcac
    12121 aaactcaatg gatatagaaa gtaaacatac acccatgatg cagcagtatc ttcgcatcaa
    12181 agctcaacat ggagacaaat tattgttcta ccgtatgggt gatttttacg aattatttta
    12241 cgaagatgca gagaaagcag ctaagcttct tgatattaca ttgacccagc ggggcatgtc
    12301 tgctggcgaa gcagtcaaaa tggctggtgt tcctcaccat gctgcagagc actatcttgc
    12361 caaactcgtt aagcttggag aatcaatcgc tatttgtgag caagttggtg atcctgcaac
    12421 cagcaaaggc cctgtggcac gcgaagtaac tcgaattatt acgcccggca cgctaaccga
    12481 tgcagcacta ctcgatggaa aatccgatag cattctagtg gcgttgacat gtcacaagtc
    12541 tgtgctaggc cttgcatggc ttaatctcgc ggcgggtcaa ttctgggtgc ttgaaacaac
    12601 gctagacaat ttgctcagtg aaatagaacg tatccgtcct gctgaaataa tcatcccaga
    12661 gtcattcgat attccaactg aactcaaaga atatggcgta atcagacagc ttccgcccaa
    12721 tcaatttgat tttgataatg cagtacgaaa tttaacgaca ctatttagaa cgcatgatct
    12781 ttcgggattt ggctgtgatc agttgcatgc ggcacttcaa gcagccggtg cgctttatca
    12841 ctacctatac cttacccaag gtactaatct cgcgcatatt gctgggttga aaatagaaca
    12901 tgacagcgcc tatatccgta tggacgctgt tacacgtaaa aatcttgaaa tctcagaaac
    12961 attgagcgga gaaacctctc ctaccttaat gtcactattc gatacttgcg ccaccaatat
    13021 gggaagccgc ttactcaagc actggcttca tcatccactc agaaacgatg caatgattca
    13081 aaatcgcctg gaaagcgtag cgttcctaaa aaataatcta aaaggtgcgc cacactttac
    13141 tagaatcaag caattgctta gaccgatagc agatatcgaa cgcattacca cgcgcattgc
    13201 cctcaaatct gcaagaccgc gcgatttatc tagcctgcgc gatagtctca ttgcacttcc
    13261 tgaaatggct gaaaccatcg ccaatcacct caacgcccat ctcaatcaat tagcgaatgc
    13321 tttaataccg gatgcaaatc tggtcaagtt attacgccaa tcattgcaat cggaacctgc
    13381 gctattatta agagaagggg gagtcatcgc cgatggattt gatgaagagc tcgatgagtt
    13441 gaggatgctg catagcaatt gtggagaatt cttactacaa ctagaaacga gagaaaaaac
    13501 acgaacggga attcccacgc tcaaagttga atacaatcga gtgcacggat tctacattga
    13561 ggtcactcat atccatacgg ataaaatccc ggctgattat cgccgtcggc aaacactcaa
    13621 gaacactgaa cgctacatca cccctgaatt acaagcattc gaaaataaag ctctggcagc
    13681 taaggataag gcgcttgcca aggagaaaca actatatgaa gcattattgg agcagttgac
    13741 tccgttcatt actcttatgc agcaaatcgc tcggagcatt gccgagatcg atgttttaag
    13801 tacctttgcc gagcgggctg agacatttaa ttatgtcatg cccaccctga cagaagaagc
    13861 aagcattacg attcaagacg ggcgccaccc tgtggtagag acccaagttg atcaatatat
    13921 cgcgaatcac ttaaatttag gcgcatcgag caacgataag aaaatgctaa tcattacggg
    13981 ccctaatatg gggggtaaat caacctatat gcgccaaact gcactcatcg tcctgctagc
    14041 gcattgcggt agttttgtgc cagcaaattg tgcgcgtatc ggtccaattg atcagatctt
    14101 tactcgaatc ggcgctgctg acgatttagc aggagggcga tcgacattca tggtcgaaat
    14161 gaccgagatg gccaatattc tcaattatgc aaccccgcag agccttgtat tagtcgatga
    14221 gatcggccgc ggcacctcta catttgatgg cttagcgctt gcttttgcga ttgctcgata
    14281 cttattgaac aagagccaaa gctatacgct atttgccacc cattattttg aactaacgaa
    14341 tcttgttaac gaatcagaac gaatcgcaaa tgttcattta ggcgtcgtcg aatatcaaaa
    14401 tcggattgtt tttttacaca ccctcaatga ggggcctgca agccaaagct atggcatcca
    14461 tgttgcagag ctggcaggtg ttcccaaacc gatcataagg gaagccaaga aaattctggc
    14521 ccatttagaa cgcgaagcaa atcaccaaaa cccacaaccc gacctatttt cagcgatcag
    14581 ccaggatgaa acgattgatt cactccccct ctctcaccct gtgatgagtc atttaaatac
    14641 tttaaaaccc gatgaactca gccccaggga agcattagtg gaactctata agctaaaatc
    14701 attactcgat gaacaatgat ggtgagcagg attacccata attgctgata aaaaaccaat
    14761 ccagcaatct agtttcttaa agttaccttc tctatttgtg gcattaaata gtctcgttat
    14821 aatgatagcc aacttgggaa gttatctgaa aaaataagga gatatcatga acgattccgt
    14881 attagatttc atttgtgacg taatagattg cgtcatttcg aacgtctaat tctagttatg
    14941 tttcttagcg agtggcattg ctgaacaatg gcagtacgta tcactcctcg tctcgttgag
    15001 ctgacttacg aggctgctct aaaggctttc tggcgaaagg aggcactccg caagttcttg
    15061 cgggcgtctc acgtcgcgga gacgtttctt gccacttggg cgccggagga aagcaagcgc
    15121 gacttcctcg acaagacgtt tcaggagctt cagcgaacgg accgaggaaa ggcagttatc
    15181 ttcgaaatgg ctcgctatct ctccgagcag acgacttttc cggacctccg caactgggaa
    15241 gactctgcgc agaagatcac ggacgccaca aaggcagtcg cggagttaaa ggcttggcta
    15301 aagacgcaag acgaagagat tcgaagcgaa aaggaacgag aggaagccaa gaccaaggcg
    15361 agagatgagc agcagaagat tcagcgaagc ctcaccgaca aggcgaagct acaacaacgt
    15421 ctcgacgcgc ttcatcccgc cgtgggcacc caacaaggtg gttacgattt tcagaattgg
    15481 ttctatgacc tcttggactt ctgtgagatc cagaatcgcc ggccgtatgt cagtgccggc
    15541 aggcagatcg acggttcgct gacattcgac ggtactacct acttggttga gttgaagttt
    15601 acgggaagcc aagctgacgc tacagacatc gattcgttac gttccaaggt ggacgacaag
    15661 gctgacaaca ccatggggat aatggtctcc atatccgggt actccagcgt cgcactgcgt
    15721 caagcatccg gacgaaagac aacgctcctg ctcgtcgatg ccatgcatct ctatctcttt
    15781 ctgtcgggca cgccgtcctt cggagacatc atctctaggc tgcggcgcca tgcctcgcaa
    15841 actggagaag cttatctagc ggtgggcaaa tttggtgggt aggaggcata acctccgcca
    15901 gcaggttatg cgggccgtta ggttttgtaa ttccatcata taagagtaag gggtcaggtc
    15961 ttgtaggttc acaaaaatgg cacggaatat aaaggaactg ttgatcaagc ttcgtcgctt
    16021 tttgcaaaac aaacatcagt tagaaattta cacgtgttcg acgtcctcaa acgaatagcg
    16081 aggctgaacg cggcattaaa actgtcttgg aggtgtggca tgcacaaacc gttttacctt
    16141 atgaaaagat cttgagttca gccttgtcag attcgtcaac ttctataata ctgtgaaacc
    16201 tcatgctgga attagcagca tcactcggca tgaaaaatta acaaactttt acttcggatt
    16261 gtaaccaacc ctaacgattc tcacggctta agtgtttatg tagccgtcta aaattaggaa
    16321 gtgggatcgt ttaatggact gataaaggtt caaattgtaa tgatacctga ccgaaagcaa
    16381 agtactttag ttctggtttg taagcgacct aaaatgggag taagcaagca acgattagcc
    16441 gttaaattgg gtcgtgagaa aacgttggtg attgcaaaag cgctgcttga ttgtgctttg
    16501 gaggatgccg cagagtggca agggcctatt gtcatagcgc ctgctgatga tgccgattct
    16561 gagtgggctg tttcattact acctgaagca aggcagatcg aggtttttcc tcagagacta
    16621 ggtaatctag gacaacgatt aaatgcgctt gaccaatcgt tgcgctgtgt cgggcatact
    16681 cagctagtat acatcggtag tgatgcgcct gtgctgcaat cggtggatta cattgcttgc
    16741 caacaggcat tgcaacatta cgataccgtg ttgattcctg caagtgatgg aggagtagcg
    16801 cttatggcga gtaatcttca ttggccagag ttagcagact taccctggag caccagtcaa
    16861 ttaggaaaag cccttgtcgt gcgttgcgag agtgctgggc aatctgttgc attgctcgaa
    16921 caacgtgctg acgtggatga gtttgcagat tgtgtaaaat taatatcaaa tttgaacgat
    16981 gatccgcgac ccgcaagaca aactttacta aaacgcatca aaagcattat tgacccttaa
    17041 aacatcatgc caagcctaag tattattatt ccttgttatc gtgatcacgc tcaagtcgca
    17101 gtgttgcttc aacagctcag aatgttatcg gctttaccca atgaaataat tgtagtagat
    17161 gcagcgggtg attcggcatg tcaggcgatc tgtatgcagt atgatgcgca atggcatcac
    17221 agtgagcctt gtcgggggaa gcagttattg caaggtgctc aagttgcgac gggtgatatt
    17281 ttgtggtttc ttcatgccga tactcgcgtt tcaccgaatt cgatttcaag cattatgcag
    17341 gcggttgagc gtggcgcaat tgggggttat tttcgctttg tattcgatgg accccatgct
    17401 tggcctatgt tgattctgga acctgccatt gcactcagaa gtcgtttcgg aacgccttat
    17461 ggtgatcaag gaatttttgt cagaagaatc gcttatttca aagtgggagg tcatgcaccg
    17521 tggccacttt tcgaagaagt tcctttagtg aagggattac gcaacttagg acattttcaa
    17581 cctttattgg attcaatctg ggtagattcg cgacgttggc agcgcgatgg ctggtggcat
    17641 cgcacattgc ggaaccgcat gttagcattg aaatttatat tgggcgcatc gcctgataca
    17701 ttagcgaaat cctaccattc tcaatcgtct taaaggagaa agctatgaga gaggttgttg
    17761 aagcgtatta tggcgaaacg ttaacgtgtt cagctgattt gcagactaat gcctgctgta
    17821 ccgatgaggg catgccgagt catttaaagc cattaatggc caagattcat cctgaaattg
    17881 caaatcgtta ctatggttgc ggtcttatag cgccaccgct gttaactggg ttaacggtgc
    17941 ttgatctggg ttgtggtgct gggcgtgatg cttatctctt gtcacaaatg gttggtgaac
    18001 aaggccaggt catcggggtt gatatgactg aggcgcaatt agcggctgct acccaatacc
    18061 aatcgtatca tcgcgatgtt tttggtcatg cagcgagtaa tgtacgattc ttacagggtg
    18121 atatcgagca ccttgatgag cttaatctag ccgatggcag tgttgatatc atcgtctcga
    18181 attgtgtgat caaccttgcc cttgataaat cggctgtttt aaaggaagcc tttcgagtat
    18241 tgaagccagg tggggagctc tatttctcag atgtttatag cgatcggcgt atacctgcag
    18301 tactagcaag cgatccgatt ttgtatggag aatgcttggg tggggcactg tactggaatg
    18361 actttttacc gcttgcgaaa cagtgtggat ttatcgatcc acggctggtg agtgatcgac
    18421 caatcacgat tggaaatact ttacttgcgg ataaagtagg gaatattcga ttttattcgg
    18481 caacctatcg cttgtttaag ttaaatgatc ttgagccggc ttgtgaagat cacggtcaat
    18541 cggttgtata tcgaggatcg attcccgatc acccgcatca ttttacgctg gataaacacc
    18601 atcggatcga gactgggcgt cagtttcctg tttgtggcaa cacctttcgt atgttgcacg
    18661 atacccggtt tagcgatcat tttgattttt atggcacatt tgatcggcat tttggcattt
    18721 tcccgggttg tggtatggga gtcccgtttg atcaagacga tagtcgtcag cctagcagtg
    18781 gctgttgttg acgatggatt tatcaatccg caatgtccat aagagagaca tgtcgcgttc
    18841 agatgattgg tatcaaaccc ctcaaggtga gccgcgtggg tttattgaat cgcgcaagct
    18901 agaggaagtt tggtttcata ccggtacagc ctgtaatctt gcttgccctt tttgtttaga
    18961 aagctccaaa ccaggagata atcgattaca gcaaatacga tttgcggatg tagaaccttt
    19021 tatccatgaa gcgcttacgc tgggtgttca acagttttca ttcaccgggg gagaaccgtt
    19081 tgttaataag gatttcgtgc gcatacttgc aactgcattg tcgtatcgac cttgtttagt
    19141 gctgaccaat gcaacaacgc ccttgatcaa acgattacat cagcttgcgc cgctgttgaa
    19201 acagccacac tcacttcatt ttagagtgag tctggatcat tttgatgagg tgcaacatga
    19261 tgcagggcgc ggtcaaggga tgttcaaact tgcgttagag gggatgcggc atctctatgg
    19321 tatgggtttt accgtttcga ttgcgcatca aaccattgca aattgcgata cagcgacggt
    19381 tgcaagatgc tttgaagaag tactgacggc tcacggtctt ccaggtaatt tacaccgtat
    19441 cgaatttcct gaatttcatt ccccggcatc tcaggttagt gttcccgtta tcacgccgca
    19501 ttgcatgaat acgtatcaaa ctgagcaaac ccgacaacgc ttcatgtgcg cttttagcag
    19561 aatggtggtt aaacaagcag agcgcatgcg tgtttatgct tgtactttag tcgatgatga
    19621 tcccgattat gcattagcag ataccttaac tgatagttta gcgattcccg ttagcttgaa
    19681 acatcatcgc tgctacagtt gcttccgcta tggtgcttcg tgtagcgagt atcgatcacc
    19741 tgcatctgtc acgattccat gaataagcgc atatgggtat tgcttggatt gctgacgcta
    19801 attggtctct atttgggttt tgatcttggg cgttttctga atttagaaac attcaaaacc
    19861 tatcaacatg aactagtaga gttttatcag cagcaacctt ggtgggtgat tagcgtttat
    19921 tccatcagtt acatcgtcat ggctgctttg tcattgccag gtgcaggcgt gatgtcactc
    19981 gcgggcggtg ccctatttgg gttctgggtc ggggtcccaa tcgtgttgtt gtcagctacg
    20041 cttggcgcaa cccttgcgtt tcttgcagca cgctatcttt ttggaaattg ggttgaacac
    20101 tacttgggtc atcgcttgct gaccattaat caagcgcttc agcgtgatgg tattttttat
    20161 ctgttctcgt taaggttaat tccagcaatt ccttttttta taatcaattt actgatggga
    20221 ttaacgacat taccgatgct cagtttcttt tgggttagtc ttgtcggtat gttggcaggt
    20281 accgcagttt atgtgaatgc agggacgcag cttgcagcca ttgatgatcc ggctgagatt
    20341 ttctcaccga ttattctttc ttcgtttcta ttgttggcac tttttccgtg gcttgctaaa
    20401 gcggctattg ccatcagcaa acggcaccga cgctatgcac gctggaaaaa accagctcgg
    20461 tttgatcgca atttgatcgt aattggagct ggtgcggcag ggcttgtaac tgcttatatt
    20521 gcagcagcaa cacgagctaa agtaacgctc attgaaagcc acaaaatggg aggagattgc
    20581 ctgaactatg ggtgcgtacc ttccaaatcg ctcattcgta ccgcacattt tctgaatgaa
    20641 gttagaaagg cgcaatccct gggaattaac cctgtcgcag ttgagtatca atttcaggac
    20701 gtgatggcgc gggtacaacg cgttattcaa acgatagccc cccatgattc gatcgagcgt
    20761 tatacacagc ttggcgtcga agtcatccaa gggaaagcgc gtattacctc accgtggacg
    20821 gtttccgtca atgatcaaat tttgtcgacc cgtaccattg tgatcgcaac gggtgcacat
    20881 tctgctattc ctgatcttcc ggggcttgat caagttcgct actacacatc agaaactata
    20941 tggtcgctgc aacaacctcc caagcatttg atcgtactag ggggagggcc tattggttgt
    21001 gagctggctc aagcttttgc acggttggcg attcctgtta cgcttgttgc acgtaatcga
    21061 ttgttgatgc gtgaagacaa tgaagtcgca gcattaatcg aatctgcttt gcaagcagat
    21121 ggtgtgaaaa ttctgataca gtgcaaagcg attcggtgtg aagtgattgg tggcgagcag
    21181 cgattgtttc ttcgtgatca agcaggtatc gaaaccatgt tggtattcga tgcactgctc
    21241 tgcgctgttg gtagagaggc gcgcgtgact ggatatgggc tagaagaatt gggaatcaat
    21301 cttttaccaa ctggaacgat cgaaaccaat gacaggctcc aaacgattta tcccaatatt
    21361 tacgcgtgtg gtgatgtcgt gggaccctat caatttaccc atattgcagc acatcaggct
    21421 tggtatgctg cggtaaatgg attgtttgat atgatcaagc gctttagagt ggattaccgt
    21481 gttattcctt ggtctatttt cacagaccct gaagtggccc atgttgggtt atccgagcaa
    21541 gaagcacaaa cccgtaaggt gagttatgaa gtgacgcgat ttgatctaaa agaactggat
    21601 cgcgcgattg ccgatgaagc ggcatatggt tttatcaagg tgctaacagt tccagggaaa
    21661 gatcgtattt taggggttac gatagttgct agccatgcaa gcgatctaat cgcggaattt
    21721 gtgctggcca tgaagcatgg acttggactt aataagattc tcagtacgat tcacagttac
    21781 ccaagctggt cagaagccaa caaatacgtt gcgggagaat ggaagcgcca acatatcaat
    21841 ccacgtctac ttgactggat tgaacgattt catacgtggc gaaggggcgg ggatagatga
    21901 attgagttca gaccaattga tccatgactt ttctgatgcg tttgacgcgc tctgtgacgt
    21961 ctaaaatttt ggtttgaatt tttagtttat cagggccaga aaggttgtac tctcggctac
    22021 tttggatcaa agaaataatc cgctccgctg caatcggtgg attaggcacg aattgaatga
    22081 gtatgctttc gctactcgca tcgatacgca ggatacccag tggtttcgca gcaatccgta
    22141 accgatgcga atccaaaagg gctcgcgctg gttcaggcag caatccaaaa cgatcaatga
    22201 gttcgcgctg catatcatcg agctgaccat cgtcttcgca attggccata cgtttataaa
    22261 gcacgagtct ttcatgaatg tcgttgcaat aggtttctgg tagcagcgcg ggcacatgca
    22321 atttgatctc agttgcaacc ccgagggggt gttgtaaatc gggttcttgg cctgctttaa
    22381 gcgatttgat cgcactatcg agcatcgtac tatagagatt gaagccgatt tcctgcattt
    22441 caccgctttg ggattcactc agtacagcgc ccgcgccgcg aatttcgagg tcgtgcatgg
    22501 ccagataaaa accagaaccc aattcttcca tggattgaat ggcttcgagt ctttttttag
    22561 cctgagcacc taatgcttct tcatccggtg ttagtaagta ggcataggcc tgatggtggg
    22621 agcgcccgac tctacctcgc aattgatgta actgtgccaa gccaaattta tctgcacggt
    22681 aaatgatgat tgtattcgcg cttggaatat caataccggt ttcaatgata gtggtacaaa
    22741 gcagtaaatt gaagcgttgt tgataaaagt cgcgcatgac atgctccaac tcgctttcgc
    22801 gcatttgtcc gtgagcgata tggatacgtg cttcagggag gaggcgagtt agcttatcaa
    22861 acatactttg aatggtgcta acttcattgt ataaaaaata aatctgacca ccgcgcttta
    22921 attcccgtaa gcaagcttct cgaatgatcc cttcagcaaa ggggtggacg aacgtcttaa
    22981 ttgctagcct tctttgcggt gccgttgcga tgatagagaa atcgcgtaac ccttccaatg
    23041 acatggctaa tgtgcgcgga ataggggtag ccgttagcgt cagaacatct acttccgcgc
    23101 gcatcttctt caattgctcc ttctgccgta caccgaatcg gtgttcttca tcgatgatga
    23161 ctaaccctag gtttttaaac tttacttttt cttggattaa tttatgcgta ccgatgatga
    23221 tatcaatctg tcctttactc aataaatcta gggcgagtgt ctgttctttg gctgaacgaa
    23281 agcgcgaaag ctcagcaatt tttaccggcc attgatcagc aatcaaacca aatcgatcag
    23341 aaaagttttg aaaatgctgc tcggccaaaa gggtcgtggg aacgagtact gccacttgct
    23401 ggccgtcggc gactgcaata aatgccgcac gtagcgctac ttcggtttta ccaaacccga
    23461 catcaccaca aatcaaacga tccataggtt taccggaagt gaggtcggtg atgacagctt
    23521 gaattgcagc tgcttgatcg gtagtttctt caaaaccaaa gccttccgca aaagcttcgt
    23581 aatcacgttg tttgaatttg aatgtgtgac cttgacgcga agcgcgttgc gcatatagat
    23641 taagtaattc ggctgctgta tctcggactt gttgcatcgc tcggcgtttg gctttttccc
    23701 attgtccact accaagcttg tgtagcggtg ctgattcagg ggaaacgcca ctgtagcgac
    23761 caatcagatg caaatgcgat accggaacat agagtttgtc gttgccctca tattcaaggg
    23821 ccagaaattc gctgttacct tcaccgaaat cgagattgac taatcccaga tagcgaccaa
    23881 taccgtgctg ttcgtgtacg acaggatcgc cgattttgat ctcagaaaga tcgcggagta
    23941 gggtatctgt cgaagtggtt ttgcgagaat ctcggttacg tcgcccatgc atatgggtag
    24001 cgtaaagctc gttctccgtg atgaaagcaa attggtcgga accaagttct gcgctcagca
    24061 caaaaccact ttgtatcggt cctgcgcaga gcatgaatgg gctgttgctg ctattgaatt
    24121 cagcatggct ttgacagatc ccaggggata agccgtggtc ctgtaaatac tcgtaaatga
    24181 tttcgcgccg accgatactc tcagcaatta ataaaatcct gccgccagtg gaattgaatg
    24241 ctgagataaa accagctaat ttttccagcg gattagcagc atggcggtca acccttacgc
    24301 ttggtggtga ctgtgccagc acctcatccg attgctcgag ttgctgtgag gtaatttgaa
    24361 tacgtgcgta agattttaag tggccgaaaa aattgtctgt cgttaaaaat aggtcgacag
    24421 gcggcagtaa agggcgatcg gtatcgccgc gcaataactg ataacgcgat tgagtgtcac
    24481 gccagaattg atcggtagcg gtataaacat cttgatgtaa gcaaattaaa ctgtcttgag
    24541 gtagataatc aaagattgtt gccgtttgct caaagaaaag cggtaggtaa tattcaatcc
    24601 ctgctggggt tgaaccctta ctgatttctt tatagatttt actgcgagaa ggatcaccct
    24661 caaatttttc ccggaaattg acacggaatt gtgaacgacc tgtctcatcg agtgggaatt
    24721 cacgtgcagg tagtaaccga atttcactaa ccggatagat gctacgctgc gtatcggcat
    24781 cgaaagttcg aatcgtttcg atttcatcat caaacagatc aatacgataa ggaagtgcac
    24841 tgcccattgg aaacagatcg actagcccgc ctcttacgct atattcccca ggagatagca
    24901 cttgattcac atgggaatag ccagccaggg tcatttgatt gcggaaatgc ggtagatcga
    24961 gcgtactgcc ttgcttgata aaaaaagtgt gagcagctag gaatgcttta gggcagatgc
    25021 gatatagcgc agtagttgcg ggtacgatca aaatatcgta ggccgcattc attacttgat
    25081 agagcgtcgc tagtcgttcg gaaattaaat catagtgagg tgaaaaggta tcgtaaggta
    25141 atgtttccca gtcaggtaat aaataaattt tggcttcagg gagaaagaat ggaatctctt
    25201 caaataatcg ttgagcatcg agcgcattag ccgcgataat ggctactgct cgggagggtg
    25261 ctagcgattg aatgagtttc gtcagcaata acgtatcact cgagcccgta aggccagtat
    25321 agcgcaggaa ttggccagga gccggtaaga ctattttaga ctgcatggga aaatcggttt
    25381 tttatcgaca aattgcttaa tttactatta taaataaggc gcccgcgaga ccaattgcgg
    25441 tcatgatcat tccaaaagca gttcgtttgt tcaggagtga actaccaata atgaaaatca
    25501 aacccagctt aaaaataata ttcgagatga tcgcgagcgt aatcgcggtg actgcttccg
    25561 atatttgaag cttaccgagc tctaacagcc gaaggctact caacgtgatg gcatcgacat
    25621 cggtaagccc tgagataatt gcgaccatgt ataaaccgct actgcctgca atatcggaga
    25681 gccaggtact catgaataac acaagtgcgt aaagtacgcc aaatcctgct gcagttgaaa
    25741 attcggttgg attcttggtt tctggaatag gcagttcttc ttgctgcgta aagtcacgcc
    25801 accaaaaaat ggtaacggtt agacctaaga taagaccact accgaggact ggcagcagca
    25861 tcggtaatac cttaggagaa agtgctgcac tgatcacggc gagccggatg agtacggtga
    25921 gattggcaag caagatcaca actaccgcaa gcttagctaa attaatattt tcttcgctgc
    25981 gacgtgtaaa aaccatcgtt gtagccgtgc tggaaactaa tccaccgagt acgccaagta
    26041 gcacggcgcc atagcgatga ccgataaaac gtaaagcaac gtaacctgtg agactaatgc
    26101 ctgaaatcaa taccatcatc aaccagattt gatagggatt gaatgcttca taaggcccat
    26161 aatttttatc cggtagaata ggcaagatga taaaggtgag aacagcaaat tgtagcatcg
    26221 atatcaagtc acgccgttcg agattctgtg ttagtccgtg tagctcagtt ttaaaataaa
    26281 gtaatatcgt tgtcacaatc gcgagcatga cagcgagggt tgtgtcgcca taccagacca
    26341 gaacacccag gctatagcag atcaaaatgg ctgtaacagt ggttgttcca ggatcagggt
    26401 cttcgtggtt tcttaagtta gcgataatcg tcatggtgcc gacaagcaag aatccagcta
    26461 aaagtaacca gggtgaattg gttttttgtg ataacaatgc tgctagtgtg ccgagcatcg
    26521 agactaatgc aaatgtgcgc aatccagctt ttgcagcttg acttcgctcg cgctccaggc
    26581 cgattaagag cccgattgcg agactggttg caaaggttag aaaatgctca agttctccac
    26641 ctggttggaa tgggctaatc attttttatt tcgttattat tatctgacca gtgttcaatt
    26701 aatagttgta gtgtcgtgtt tccattgaat tcattcgctt gcaagcgaaa gacagcttga
    26761 atacattccg gtaacggttc agcctggaaa aataaaatcg catcatagct ggtatttgaa
    26821 atcgattttc gcagttttaa ttttaaatgt ttttcaccta cgatacgttg attttccaca
    26881 taaaaccgcg cattaaaggt tggctcagga aaaccatgac cccaaacaac ttcggcaaac
    26941 gattgcgcaa gcgctaaact taattcggtt tcgtttaaat ccccatcggt ttcaatctgt
    27001 tgcgttaaat cagccggagt caacaattgt tgagctattt gctcaaaggc tgcttgaaaa
    27061 cgttgaaaat cgtgctccat gatggtcagt cctgcggctg cagcgtgacc accaaacttt
    27121 ttgatcaatc ctgggttacg tttggatacg agatcaagtg cgtcccttaa gttgatgcca
    27181 gcaatcgaac gtcccgaccc tttgatttcg ccttcattcc ctggcgcaaa aataatgatg
    27241 ggccgatgaa atttttcttt gacgcgagaa gcaatcaatc caatgacgcc ttgatgccaa
    27301 ttaggatcat agagacaaat gctatagaca gattcggcgg gttgttgaaa ttttatattg
    27361 agcgctagct gatccagtgc agaatctcgc atggttgctt ctatttcgcg gcgttgccga
    27421 ttgagcctat ctaattcact tgcaatttca aacgctcgac cttcatcatc ggtaattagg
    27481 cattctatac ctaacgtcat atcatccaat cgacctgctg cattgagtcg tgggccaatc
    27541 acaaagccaa gctcattcgt cgagatacgt tgatagtcgc gttttgcgac tttgagcaac
    27601 gcgtagatac ctgtgcaggc tttaccattg cgaatacggt gtaggccatg tttgaccaaa
    27661 actcggttat tccagtcaag cttaacgaca tctgctactg tacctagtgc aacgagatca
    27721 agtaggctag cgaggttcgg ttccttaaaa cagttattaa aaacgcctct tttgcgtaat
    27781 tctgcgcgaa gtgccagcat gacgtaaaag atgaccccga cccctgctag atttttgctg
    27841 gaaaatgggc agctaggttg gttcggattg acgataacgg ctgcattggg gaggtgttca
    27901 cctggtaagt gatgatcggt aatgagtacg cgtattccta aacggtttgc ttcttcaacg
    27961 ccttcaatgc tgcttatccc gttgtcgacg gtaatgatga tatcaggtcg atattcggaa
    28021 ttactggttg cagcaaggtt gacgatttca ggggttaggc cgtagccata ctcaaagcga
    28081 ttgggtacta gatagtctac acaggctcca aattttctaa gtgcccgaat agctaatgca
    28141 caggcagttg ccccatccga atcataatca gcaataatga gtaagcgttt ttggtcggcg
    28201 atggcgtctg ctaggactac tgccatctca gcaatatttt ttagctgctc aaatggcaat
    28261 aattttgata acgccgtctc taattgactc ggttgttcaa ttccacgtgc tgcgtagata
    28321 cgcgctagaa tcggggagca accttgctga actagccaat caaaaatagc ttgagggaaa
    28381 acacgggtag taatattagg catttgccta atagtttagc atggagtaaa acaggattta
    28441 gcgggagctt cagacccatg aacacgggcg tgaatcattt taatcagtct gatctgatgc
    28501 ctcagggtta gcagcgtcga tcttttcgac cttgagtaaa tgcaggcgcc gactatcagc
    28561 gcggagtacc gtgaatcgat aatgatcgat aatcacagac tcaccattct taggcattct
    28621 accgaattta ttagtcacga cacctccaat cgtatcaaaa tcttgatcac tgaatgctgc
    28681 gccgagtgtt tcattaaaac tagcaacctc agttatggct ttgacacgat aatgcccgtt
    28741 cgtctctgca acaatatttg cttcgttctc atcgaaatca tattcgtctt caatatcacc
    28801 gacaatttgc tctagcacat cttcgatggt taccaggcca gcgacaccac catactcatc
    28861 cacaacaatt gcgatatggt tgcgattgct acgaaagtct tttagcaaga tgtcgagtcg
    28921 cttggattca gggataaaca cggcgggacg cagcatttca cgaacattaa aactctcttg
    28981 ctcagaatag taccgcagca aatccttagc gagcagtatt cctatcattt gatctttact
    29041 tccatcggtt actgggaaac gagaatgccc cttctcgatg acaaaaggga tgaattcagc
    29101 aggtttctca ctgatatcga tggtatccat atgggagcgc ggaatcatga tgtcgcgaac
    29161 ttgcatttcc gacacatgca ttacaccttc aatcatggtt agtgcatccg catcaagcaa
    29221 attcctttcg tacgcagaat gtaagagcgc tactagctgt tcgcgatctt ctggttgtcg
    29281 catgaggagt gaaccgatat gctcaaacca actgatcttg ggagaggaat gattcatgat
    29341 tagtctcatc atttataagt tatgtgttat ttggatgtag ctaaaacaag ttattaatat
    29401 ctgcaacgct tatctaacta ataggggtct ggatagccca gttgagtcat gagctgtgat
    29461 tcgagctgtt ccatatctac cgcctctaaa tcagtttcat ggtcataacc ctgaaggtgc
    29521 aatacaccgt gaatggtgag gtgtgcgtaa tgggcgtcaa tacttttgtg ttgttcttgc
    29581 gcttcacgaa ttactaccgg cgcgcatagt acaatatcgc cggctaaagg cgatgagtca
    29641 tcataggtaa atgtcaaaac attggtagga taagcttttc ccctaaagcg ctgatttagt
    29701 gataggccct cttcttgatc aacgatgcga attacgattt ctgcgctttg tactaatgct
    29761 gctttgaccc atttacgaaa atggtggcga ttcggtgttt cggggaactt tgaaacatat
    29821 tgaaccatca gcgataattt attcgaggtt ttctcaacag tattgctcgg aatagagata
    29881 ggtttatgct tgttcgctgt gctgctcata agcattaaca attctttgta cgagggggtg
    29941 acgcaccacg tcttctgatc tgaaatgagt aaaagcaata cctttaattt gctttaacac
    30001 ctgctctgca tcaattaatc cacttttctg atgtttgggt agatcaattt gagtgatatc
    30061 accggtaatc actgctttag atccaaagcc gatgcgtgtc agaaacatct tcatttgctc
    30121 gggtgtcgta ttttgtgcct catctaggat gatgaaagat tgattcagcg tgcggccgcg
    30181 cataaatgcc agtggtgcaa tttcaattgt gttgcgctcg aattgacgac tcgttttatc
    30241 aaatcccatt aaatcataca gtgcatcata aaggggacgt aggtaaggat ctactttctg
    30301 aactagatcg cctggtaaaa aaccaagtcg ttctcctgct tcaacagccg gtctcactag
    30361 aatgatccgc gataccattt ctcgttcgag tgcatcgact gcgcttgcaa ctgcaagata
    30421 agttttacct gtacctgcgg ggcctatgcc aaaaacgata tcatgatctt gaatctgttg
    30481 gaggtattgg ccttgacgta aacttcgcgc atgaagatta tttcgacgtg tcatcaactt
    30541 tggtgatgag gtggcgtcaa ttgcaggatc aatcgactgg ctcagttgac tcgataaatt
    30601 agcggcttcg atcaagccta attggacttg ctcaatactt aactgatgat gggattgatt
    30661 ataaaaattc ctaagaactt gcgctgcgat ctctgtttga ttggattgcc ccttgagaac
    30721 aaaatgctca cctcgtctgg caatattaac atctagggca ttttcgattt gtttgagatt
    30781 ttcatctaat gccccgcaga ggttagcaag gcgctgatta tccgtggggg agaaagaaat
    30841 ttctacaggt ttaggtttca aaacgataat taattgaaaa cgattagcgc attctcgcag
    30901 gggttaaatg agtttgcaag tgttaaaagc tatcctctta atcgtttatt tcagaatttc
    30961 tccacgcagg gaatggggca acgccgctgt aattttaata tcgatgaact gaccgattcg
    31021 attcggatca ccaataaagt taaccatgcg attattttct gttctaccgc ataattcgtc
    31081 tgcacttttc ttagaatagc cctcaataag gatacgttga gttgtgccaa ccatattatg
    31141 actgattaaa gttgcgtgtt gcgcaatttt ttcttgtaaa cggtgcagtc gctctagttt
    31201 gatttcttga ggtgtatcat ccggtaagtc agcagcaggg gttcctggtc gtgggctata
    31261 aatgaagcta aacgattcat cgaaataaac tgcttcgatc aatttcatgg ttgcttcaaa
    31321 gtcagcctct gtttcacctg gaaagccaat aataaaatca gaactaatcg agatattagg
    31381 acgtaaagcc cttattttac ggataatgga tttgtactcg atcgcactat agcctcgttt
    31441 cattgcagcg agaactcgat cagaacctga ttgaacgggt aagtggaggt gattcactac
    31501 tttgggtagc tttgcataaa catcgatgag tcgagttgta aattccttgg gatgcgaggt
    31561 ggtgaagcgg atacgttcta tatctggaat ttcatgaatg aattcaagta ataatgcaaa
    31621 atcgacaatt tcgccgctgt ccatttcgcc gcgataggcg tttacatttt ggccgagtaa
    31681 gttgacttct ttaacgcctc gcaacgttaa atgggcaacc tcaaccagaa tgtcattcag
    31741 tgggcgtgat acttcttcac cacgggtata gggaacaaca cagaagctgc aatatttact
    31801 gcaaccttcc atgatagaaa caaaggccgt agccccttcg gtgcgcgcag gaggcaaatg
    31861 gtcaaatttc tcgatttctg gaaaagaaat atcaacttga gaacgacccg ttaattcacg
    31921 ctttctgatt aattcaggta aacgatgtaa ggtttgagga ccaaacacga gatcaacaaa
    31981 aggggcacgc ttaattatct catgcccttc ttggctagcg acgcatcccc ctacgccgat
    32041 tagcaagttt ggattcttat cttttaaatg ccgaaccctc ccaagatcat ggaaaacctt
    32101 ttcttgcgct ttttcacgaa ctgagcatgt attgaacaag ataacatcgg cttcttcagg
    32161 tttatcggtt aactcaagac ctttagcgga atgaaggaca tcagccattt tatccgagtc
    32221 atactcgttc atttggcaac caaatgtttt aatataaagt ttattattca ctgattaaaa
    32281 aaattaaact gcagaaagca gaacaattat ttagtatata tgggttgtgc tcaggattct
    32341 atgcttatcc ttaggcaagt acttactaca gggttacttt aattccttct agccgcaacc
    32401 ccaaattatc atgcctgatt cgataaataa tttaacttcc agttcacaag acaaagatgg
    32461 tctgcatatt ctgcgtctac atgattcatg gattatgcgc tggctttacc tgggagtggg
    32521 tatcacagcc ttattgctgg gtattttagg gatatttctg cctattttgc cgactacccc
    32581 ttttatttta ttagcagccg catgttttgc aaggagctcc gaaagattcc atgtgttttt
    32641 actcggtaat cgtatcactg gaccgattat ctacgagtgg tgcatgcatc gcagcattcc
    32701 acgtcgcgtc aagcgttggg tttatttgat gattactttg tcttttggaa gctcgatcgt
    32761 tattgtgtca gcgatatggc tcaaactaat gctagtaatt ctagctatcg tgctgactct
    32821 atttatttgg cgcattcctg taagggatag agccgaagct ggttagcgta acagaattaa
    32881 ccgaggaact gcatcaacat tacaccaaac gattgcgttt tgtttgaatt gtatcccgat
    32941 ttgaatcgaa tgaagtaagt cgattccaag cgtaagataa ctggattctg ctggccagtg
    33001 atttcgtgga tcaatactct cgcctgggta aatccagggt gaataatagg ttaattgctt
    33061 atgaagttgg atttgtaata gatcattgat ttgagtgcat tgaagttggc tatgaggatt
    33121 gcaagcagtg ataaatagcg cattggttag ctttgctcca atcatcaagc gtgaaagtat
    33181 gtcacaatat tgaccaattc tgaacgtcat ttttccaaag tggctatctg tttgatagaa
    33241 ggcactaaga tagttttgga ttagttgggt tgagagcgcg ctttgcgtta agcttttttc
    33301 tagaatgatc atttgacgct gataaagatc agcaaattgg gatgtctgca tatcgaagga
    33361 aaggtgttat taggatatga gtgagcacgt atgaagctgt ttattttaat cattttattt
    33421 ttctttgcgc taaacgtgat tgcagaggaa tcatcacgta atattgttcc tgataccatg
    33481 actgagcgcg ttagggcatg cacgatttgt caccaggatg gagaccatga tcaagcgagg
    33541 agtacttatt atcctcgcat cgctggaaaa cttgaagggt atttatttaa ccagttacgc
    33601 cactttcgag atgggcggcg atatcaccaa cctatggcgc tcttacttga gaatatgtct
    33661 gatgactatt tgcaagcgat agcacgtcat ttttctcaat tgaatgttcc gtatccttca
    33721 cctgaatcga ttgatttaaa atctaacgaa attcaattag tagaaaaact cattcggttg
    33781 ggtgatccac aacgcaatat accagcatgt agtgcttgtc atggtgatac cttaatgggt
    33841 aatacaccgt atattccagg tttattgggc ttacctagcg cttatttgac agcgcaattg
    33901 agtggctggc gaagcggaag tttgattcgt gggcaagtaa cgaattgcat gtctgaaatc
    33961 gctaagcagc ttagctatga tgagattatt gctatatcga gatggttggc tactcaagct
    34021 gtagaaggag aagctcaaaa agtgctttcg cccgatttga taaagcgatg tcaatcgatt
    34081 tttctggaag gagtagcgca agaatgagcc cttattactt ttgttttact tcgggttttg
    34141 ctagaaagtg cgctctaggt tatctcgtat tttttgtttt gtttggagtg caaataaacc
    34201 tgatttctgc tgctccggga gaacaggact tagcgcgagg tgaatatctt acgcgagttg
    34261 gaaattgtct aggctgtcat acagcaaaag gaggcgttcc atttgcaggt ggacggcgat
    34321 tgtcgactgc atttggtacg tttgtgacgc ccaatattac gccggataag gaaactggaa
    34381 ttggccattg gaccgaagag gatttttggt tagcgatgca tgagggaaaa tctcgagatg
    34441 ggcgaatgct ctatcctgca tttccttaca ctgaatttac caaggtatca cgagctgatg
    34501 taaagtcgat ttttcaatat cttcaatcga ttgctgcggt ttcccaggtt aacccagcac
    34561 atgagattcc ttttccttat aactctaagc ccttgttatt tgggtggcgg aagctttttt
    34621 ttaaacaagg catttatgaa gcggatccaa cacaaaacga agaatggaat cgtggcgcct
    34681 atctggttca aggattaggg cattgcgatg cgtgtcatac agcgcgtaat gaagtcggcg
    34741 ctaaaaaaga tgctgcagtt ggaggtgggc aaatgatggg cgcaaactgg tacgcacctt
    34801 ctctttattc ccgtaaagag gcgagtgtta ccgactggag tcttgaagct attatcgaat
    34861 tgctttcttc aggcgtatca aagcatgcga atgtttctgg tcctatggca acggttgtgc
    34921 agcaaagttt gcaatattgg tcgcctcaag atataagagc catggcagtc tatcttaagt
    34981 cgttaccaga taaaactgca caggaaaatg ttcgctatcc agaaatgcct gagagcgcta
    35041 agcgatatct tgcgcagggt gagaaagtct atgagaagca ttgccaagat tgccacggcg
    35101 aggttggtca aggcatacca ggtatctacc ctgcgcttgc taataatcgc agtgtcacct
    35161 tacccgttcc gcttaatctc atcagagctg tgctaaatgg gggatatcca ccgactactc
    35221 atctcaatcc acaaccttat ggcatgccac ctttccaaca attgctcaga gatgacgaaa
    35281 ttgcacaggt gatttcatat atcagaaatg cctgggggaa tcgagcgggt ttagttacga
    35341 ttgtggatgt ggatcagggt agaggcggca atcattgaag ttggagtggc tataagaatc
    35401 tgagcttatg ctcctttagt ttgggatgat tcggattgcg catgatgttt ttctttgtgt
    35461 aatttccact tgagg
//
LOCUS       DAOQLJ010000005        33925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_400_length_33925_cov_9.121760, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000005 DAOQLJ010000000
VERSION     DAOQLJ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 33925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33925
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_400_length_33925_cov_9.121760"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PK522_00715"
     CDS             <1..651
                     /locus_tag="PK522_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40284.1"
                     /translation="KQFSEKLSAVLTSSLTQTYTVVYNRTTRKITISAPSNFSILIGS
                     GSHTTTTLYENLGYTGGIDLSGFNSYVADSTAGYEYNPQFYLLDYIPLEHNVKSVQAS
                     INETGSGAIEVIRYGTKRYTEFSIELITNNKFVGDSIWVSSKTGVEDTLNFLNYATQK
                     SRIEFMPDGYDPATYYTLILESTESDQNGTGFKLMEMLEYGAGYYKTGKLVWREVT"
     gene            653..1483
                     /locus_tag="PK522_00720"
     CDS             653..1483
                     /locus_tag="PK522_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40285.1"
                     /translation="MSVVNNQDATAEVFNAAFASKTADNTSTGKQTLSRVGSGASVVD
                     VQQAINDNIADIASAQSDIDIHVADVANPHSVTKAQVGLGAVDNTSDLDKPVSTAQQA
                     ALDLKIDLTEKGAANGVVPLNGSSLIDSTYLPSYVDDVLEYANFAAFPVTGETGKIYV
                     ALDTSKIYRWTGSIYVEVSQGVTDHTQLSSIGTNTHAQIDTHIASTSNPHSVTKTQVG
                     LSNVDNLQQLPISYLDTDGTLAANSDVKVASQKATKTYVDTQILANSGSGIVTALIFG
                     "
     gene            1493..2302
                     /locus_tag="PK522_00725"
     CDS             1493..2302
                     /locus_tag="PK522_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40286.1"
                     /translation="MKKFITPAYTFTPGGSGVGTVDLSGISGFNVKNLVAVINQTRGV
                     VIYATGQSSTRYTNVTGTTVTLFYDTTGQSGSDVLQVIYEDNSTDQPVSVSSSALPTG
                     AATSANQNTGNTSLSNIDTATTGINAKLNSNFGASSNAVRVAAQLGNATAIADFAAGN
                     STAQTLRTVIATNQPAIATKSPVNASGSTVSASISAVTTVTAPANAVEVIVQADDTNT
                     ANLRFAVNGTATTTSGIQLQPGRSETLKIGSDISIIPESGTQKYNIIWVTQ"
     gene            2299..4233
                     /locus_tag="PK522_00730"
     CDS             2299..4233
                     /locus_tag="PK522_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40287.1"
                     /translation="MIRQLILALLISTQAFAGLPPTTSKGISDTANQTTFNFQFPQLV
                     PTRTGTTTSLSPPENGAFVKNYIRNGNAESGATTGWAVYDDGAAVPVDGTGGSPVLTL
                     AASNSGPLAGTYSYLNSKGNAISGFGNGFSYDFTIDQSDTAKVLQIQYDYLLTGAYTL
                     GSDSTTGTWVTYLYDVTNAQLIQPTSYKLLGGGRFIGNFQTNSNSTSYRLIIHNSQVQ
                     LSPATFKFDSVIITPTVYAFGTPITDWQSYTPTFTGFGTAASIECQWRRVGSNVELRN
                     KFTTGTPTGVEARVSLPTGLTIASTSTVPSIQPLGQVYRGTTGAANIYYAQATAGLTY
                     LTIAKQDSVGPFASTLGTSFGSAEVVYLQSSVPIQGWSSSVQTSDQADSRTLIEAYNN
                     NAGTVLTANTTNIDFSTKVTDTHGAWSGTVFTANAPGNYSIKGSISCTTGASRAFYAY
                     VNGTQNKLVAVTTTGNVTPFVADTFLNANDTFSLRSNASCTLNAVATDHWINITRSPS
                     SSVISATETVAASYYASANQTTAAQINFDTKEYDTHGAVTTGAGAWKFSAPYSGLYSI
                     NVTGYSTSNTNVSIFKNGSNYKYLFYTVPSENGRTGSAQVRLSAGEYVDIRPSASVQI
                     NGGAQNAVVTSRIEILRVGN"
     gene            4243..4626
                     /locus_tag="PK522_00735"
     CDS             4243..4626
                     /locus_tag="PK522_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40288.1"
                     /translation="MLCDFSNLESDSRFELIRDGYLFALSRHKKEVLIYSLRMNWICD
                     LLEKSKIGKENAKKIYTKSYDYLIKNDYNRFIKYFAFQICSLASDLCDEFNCKEKMDD
                     DFKKFESLIVVYSEDIEEIKAYIGG"
     gene            4629..4961
                     /locus_tag="PK522_00740"
     CDS             4629..4961
                     /locus_tag="PK522_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40289.1"
                     /translation="MATYSNNTTIKIGSPVAIRNTGSGSTTYTVPNNCHLDLIYTNVD
                     GAGSGLNFYIKSSANTFIYNYTGNTTQTHLFENSGLRLPAGSVIYMDGSVAKSVREII
                     GTLFINTP"
     gene            4958..5191
                     /locus_tag="PK522_00745"
     CDS             4958..5191
                     /locus_tag="PK522_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40290.1"
                     /translation="MKMIDEKTGVPMFAVITAVPTLFGFIIWLSSISFTANSAKSEVA
                     ELKSKQNAQNELLLNIKEDLTLIKYKLEIKEKR"
     gene            5191..5412
                     /locus_tag="PK522_00750"
     CDS             5191..5412
                     /locus_tag="PK522_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40291.1"
                     /translation="MDVLNHLIEVVGNAANAPIMLSIGIAVEFAFRMFKTEKPKSIIY
                     MVADIIKKSGELLTKVGTLLDKVLPQRLK"
     gene            5414..5611
                     /locus_tag="PK522_00755"
     CDS             5414..5611
                     /locus_tag="PK522_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40292.1"
                     /translation="MNQVSAIASALIEIITLGYKVYDLIHEAKRKGWVSDGRSLSQQI
                     AEAKTDEERAELAKRLFNHIK"
     gene            5828..6271
                     /locus_tag="PK522_00760"
     CDS             5828..6271
                     /locus_tag="PK522_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HNC40293.1"
                     /translation="MQKMNDEAPRWLQVASQELGISEIVGLDANKRILQYNSCTSLNA
                     QSDEVPWCSSFVNWCVKQSMLKGTFSAAARSWLSWGYECEPRYGCIVILRRGNSETQG
                     HVGFLVDHDDLFVHLLGGNQGNKVSIQKFAKHEVIGYRWPFKVVA"
     gene            6276..9008
                     /locus_tag="PK522_00765"
     CDS             6276..9008
                     /locus_tag="PK522_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40294.1"
                     /translation="MAYQLSSRAKTLLNQTNIEPNIVLCIGDYGCFGASITKVKVKIG
                     MPGLKIGGGWKIGGTIQDENIVDYISLDGTTTDITQQLDLDKGGATSTQTVDVRLVDI
                     NGEITRLISPGFDLNDILYTDAQLYLGFKDGAFPQDYVDLFIGKVQSIKSGSGYIDLT
                     IAHPDELKRAEIFPKFETSLVADAPYYSKSIQDLTYYQSGNYNGIVQVRYLNIPFIGD
                     VANITVSGGLITASIESGVTKANTIKNAIRDHFEANNLVQVMVSGTGTNAQTTQATTS
                     LEVSDELFLESVDGLYLPHGDIFKTYVRIDDEIIEYTGIDTTLKKLTGCVRRSLNSLG
                     KSHKIDDAVQSFYKLGDNTDASNAIDLALMLMLSDGIGSPYLTGIEDLQFYNFGTLTN
                     YPNGLLITGVDAVRRYNIRAGDLFTVSGASNPANNFTDQVIEAVDVIDLGTIVYIAGQ
                     SFVSEPMSAALGSVKSQYNVLPDGCGFIPNQVDIDRFKQIQSRFPSSIANYELYLKDT
                     VKPKDLINTDLFLPSALYSIPRQGKASIGISAPPLYEGGTKTLDITTVKNPSKLKMTR
                     TVSKYFYNSIIYKYNEDSVDDRALSGLIIYSADSVNRIASPNRPYTIEAKGVRPSPTN
                     TQLITRNCKRYLQRFQFAAEQIPVEPDYKTGYTIEVGDSVVFGDSELQVSDSVSGTRD
                     FKPRVFEVVNKSFNWKTGKITLNIVDTNYSASVRYGVWSPASMVKAGSTTTNIKVLNS
                     YGSTSERDKWTQYFGRTVRVRSKDYSISQNVKLVGFDPTYDDTMIVDTLGFTPLDGYI
                     VDTPIYNDLNLAGDSFYKAVHPFWTPTLTVVSGTSSTEFEVSVGDAAKLFEGSTIRIH
                     NADYTVDSAEKTIKVSSVVGTTVTCDDIGFTPSAGQLINLVGFVSDDGNPYVWV"
     gene            9015..9566
                     /locus_tag="PK522_00770"
     CDS             9015..9566
                     /locus_tag="PK522_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40295.1"
                     /translation="MATEVTQIKQKIYEEDVETGAAASEETMTKFGGSLNLLLDKTIF
                     PVHFDIHGPYNITGVPDAKVDKFYCVPFDAEIVGSHFYTGENPGSSGSTEIDILRKPL
                     SGGTASIFSTRPIIPASAGAEADIIQTYLPSASTIRGATGGTDAVLSSTQLDAYDVLK
                     FSFIDKPVGTVDKCGFVLWLRAR"
     gene            9568..9646
                     /locus_tag="PK522_00775"
     tRNA            9568..9646
                     /locus_tag="PK522_00775"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9600..9602,aa:Thr,seq:agt)
     gene            complement(9647..10324)
                     /locus_tag="PK522_00780"
     CDS             complement(9647..10324)
                     /locus_tag="PK522_00780"
                     /inference="COORDINATES: protein motif:HMM:NF018122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HNC40296.1"
                     /translation="MKTFNELRKLNVSEHIEKKNGISYLSWTYAVDELLQADPTATWE
                     FLPHMNINDTVMVQCKVNAFGKSMTMHLAVFDYKNQAIKNPNACQISNSMMRCLVKCI
                     ACFGIGLYIYAGEDLPEKDESQKKIIPDQPTAEDPQTGFSTDGIYRIPSGTHKGRTLP
                     QVIDIIGQRKLEDTIISFEDRIAKKSPYSGFTMEQMQKFVSEATAFVNSLESENSEFD
                     SFMKGGK"
     gene            complement(10321..10521)
                     /locus_tag="PK522_00785"
     CDS             complement(10321..10521)
                     /locus_tag="PK522_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC40297.1"
                     /translation="MNHTPGQYIFMLRKLANMTQVELSKRSGVSQSNISKVEHDSKGV
                     MVDDFIKLLDAMGRKIKIVRKK"
     gene            complement(10518..10796)
                     /locus_tag="PK522_00790"
     CDS             complement(10518..10796)
                     /locus_tag="PK522_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40298.1"
                     /translation="MQNNNELPKIPEITFVVSKGNIREEYGSYKEFRDWFYDECDKEL
                     CQAYKEADGDSKRGCDGIKEWIDYTFTDDDYLDFLDEKGWKFERVVKK"
     gene            complement(10853..11416)
                     /locus_tag="PK522_00795"
     CDS             complement(10853..11416)
                     /locus_tag="PK522_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40299.1"
                     /translation="MKVLVACEYSGVVRDAFKKRGHNAISCDLLPTDKPGQHYQGDVF
                     DIINDGFDLMIAHPPCTYLCNSGVCWLHKDESRWDKMRLGANFFKRLLNCSIPKIAVE
                     NPIMHKYAKEIICVNQSQIIQPWQFGHGETKATCLWLKNLPLLRPTNIVEGREQRLHK
                     LPPSKDRWKIRSTTFQGIADAMANQWG"
     gene            complement(11473..12048)
                     /locus_tag="PK522_00800"
     CDS             complement(11473..12048)
                     /locus_tag="PK522_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40300.1"
                     /translation="MKVLVACEFSGTVREAFRRRGHDAWSCDLLPTEIPGNHIQGDAS
                     TILNAGWDLIIAHPPCTYLCVPGAHYLHKQNDRWGKMLSAREFFLLCLNANTKKLCVE
                     NPLPHRYAELPKYSQIINPWQFGQEFSKRTCLWIKGLPLLMPTKIVSKGERYTRKDGT
                     TSNSKWYAKASQKERSKTFQGIADAMASQWG"
     gene            complement(12106..12300)
                     /locus_tag="PK522_00805"
     CDS             complement(12106..12300)
                     /locus_tag="PK522_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40301.1"
                     /translation="MKKAKAITAYGESDISEIIKDIFSQMQSPEGQRFKAVHMLDGLV
                     MINIHGKTFLINVSEKDSLN"
     gene            complement(12297..13271)
                     /locus_tag="PK522_00810"
     CDS             complement(12297..13271)
                     /locus_tag="PK522_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNC40302.1"
                     /translation="MSLKERQDYCVKIIKELRAELNRVPTSADFKNKLPRINVTELFG
                     SHDGLLRAAGLLEAIEEFEGPQPRQPRILVLDLELKPIIAYVWGLFDQNIGLDQIIQD
                     WSIMSWAAKWIGESNVMYEDVSGEGDYSNDERIVRLMWHKLDEADVIITQNGKKFDEK
                     KLNTKFEKYKLGRPSPYRHIDTLKIKKKHFGLTSNKLAYSTDYFNETYKKLDHGKFAG
                     MKLWIECLNRNPEAWAEMKEYNIYDVLSLEELYLNSLRHWDDTINFGTYTQKAGCCPN
                     CGSEDLFEDKFNYSKTGAFQTYRCKNCGTISQSKINEIHQNTRKAFLK"
     gene            complement(13550..13798)
                     /locus_tag="PK522_00815"
     CDS             complement(13550..13798)
                     /locus_tag="PK522_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40303.1"
                     /translation="MKFKYWLCEHNQIDIAQGRMERVRLYPRKPKQCAEQWCDHNNFL
                     EVIIDVIHPKKDAKNKDKPAREKLGHIRKKRQSTQQEV"
     gene            complement(13795..14061)
                     /locus_tag="PK522_00820"
     CDS             complement(13795..14061)
                     /locus_tag="PK522_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40304.1"
                     /translation="MKIKLQNTPQFILGIIFNPHYCFSIMFGWVEFSIMFFDNKTILD
                     TLPDFPPIHWPCQKCGAEDEGQGCNMDNAGNVTNCEKCSEVILK"
     gene            complement(14140..14286)
                     /locus_tag="PK522_00825"
     CDS             complement(14140..14286)
                     /locus_tag="PK522_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40305.1"
                     /translation="MKCPKCGGEWRCGCNSCKKRNIDGKVLAVFDEDEYASCGYCGLR
                     MHHE"
     gene            complement(14283..>14477)
                     /locus_tag="PK522_00830"
     CDS             complement(14283..>14477)
                     /locus_tag="PK522_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40306.1"
                     /translation="QLTATTQALDAAVEVIEAALRLQDLWLYHGKISVEHAHEANALN
                     LMKHRFEESLEKIKALRGGE"
     assembly_gap    14478..14577
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14924..15088)
                     /locus_tag="PK522_00835"
     CDS             complement(14924..15088)
                     /locus_tag="PK522_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40307.1"
                     /translation="MIGFMIFLYMVISAFMFFAMIDEGAEVCLAWAIFWPLLLGKKAC
                     IGFVKEWNDL"
     gene            complement(15085..15444)
                     /locus_tag="PK522_00840"
     CDS             complement(15085..15444)
                     /locus_tag="PK522_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40308.1"
                     /translation="MNTTEEIKPSEPVTNKKAEYPDLSGKFRRWLLSYYSGCIGDFGP
                     LNDDDLEIAFKAGYDAAMKESVITDSMMIDFLIKNINNTYLLEINDKWIINLDFDNEY
                     ETPRDAIRAAMKESGSL"
     gene            complement(15858..16205)
                     /gene="ssb"
                     /locus_tag="PK522_00845"
     CDS             complement(15858..16205)
                     /gene="ssb"
                     /locus_tag="PK522_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNC40309.1"
                     /translation="MVNRVFILGRMGQKPEVKHTANGQAIANISIATTEKFSDKQGNK
                     QEKTEWHRVVAFGKQAEIIGEYTDKGSKIYVEGKIQTRSYEKDGITHYSTEVIVNDFR
                     LLDSKKEQQDAPF"
     gene            16486..17178
                     /locus_tag="PK522_00850"
     CDS             16486..17178
                     /locus_tag="PK522_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40310.1"
                     /translation="MITIKFNNWDKYNKRQKDIKNPFWFSMSNNFFYDPEFYDFSAEE
                     KCTFFYLLAEASRNGCYGEFNTTETHYSRITGYSKQTLYKTLDKLLKIKVAAVSRQDG
                     GVNPASTEQNKTEQDNIKYMSDFEKSDAFDFEEIYKAYPRRLGDMKKGGGINKLKRLI
                     KTQTDFDDALNAVKRYNQYCIETKKVKTELVKMFSSFFDANGDWREWITFTADKPKSS
                     MDAWLEKYKDEA"
     gene            17168..17653
                     /locus_tag="PK522_00855"
     CDS             17168..17653
                     /locus_tag="PK522_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40311.1"
                     /translation="MKRSEFAEQMGRLIDVYGEKSYPSPRTDLIYNWASKMQVESFSQ
                     IVSKLIAECDRPPLLNKFKDAHQELGILKNRPIVDCVYCGGTGFICDDAPLPTVKRCI
                     CEAGDEKTKYIARFKGFPVAAFPSQAKLAEIRQAASDRAAKLIKQTMLNKTIPNDNQQ
                     D"
     gene            17704..17943
                     /locus_tag="PK522_00860"
     CDS             17704..17943
                     /locus_tag="PK522_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40312.1"
                     /translation="MKFNNAPVDPLKIQLIHDMLNSPEVNALWLEFVHLGNVPQVKHE
                     LRAIAFNRYAKARDAFLGLPALNASALEEKFINLK"
     gene            17950..18219
                     /locus_tag="PK522_00865"
     CDS             17950..18219
                     /locus_tag="PK522_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40313.1"
                     /translation="MKTNPNDLAYPLPNHNFTDKNREQYIARLAPGLTKREYFAAMAM
                     QGLNANGELVKALAIKGDVDYPGEFIATMACNSADALIDELNKEK"
     gene            18221..18370
                     /locus_tag="PK522_00870"
     CDS             18221..18370
                     /locus_tag="PK522_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40314.1"
                     /translation="MAPLKKGYSAKTVSKNIKTEMSHGKSQKQAVAIALSEAEKAKKK
                     KKKKK"
     gene            18470..18874
                     /locus_tag="PK522_00875"
     CDS             18470..18874
                     /locus_tag="PK522_00875"
                     /inference="COORDINATES: protein motif:HMM:NF020354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HNC40315.1"
                     /translation="MKLERNALLIGGLMSKQKQLPESVIESQIFHYLRGRGVLCFKSI
                     QAGFFDPVRKVFRKQASPYFRKGVSDCLGLLPDGRFLAIEIKARYGKVSPEQQRFIDD
                     VNNSGGVAFVARSVQDVVDKLSSIIGSSTPMK"
     gene            18941..19576
                     /locus_tag="PK522_00880"
     CDS             18941..19576
                     /locus_tag="PK522_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40316.1"
                     /translation="MRKIKHWLAAAGLAIASLFGYHHTIPETRVIEVIPQPATTPTPI
                     ATPIVEEIHPKIDSKRINISCDSTCTHQEKIDLARIVAKLEAVQSSKCVTDYLKAPDT
                     ALDVKQLDGMTLDAAIQKVLTSKVSTQLTYYYEGRNWFTKTLVIGYENGDGKVHANRA
                     AWDYMSDCEKAGNLAHEISHQLGFHHDFERTARRPYSFPYVIGSAVEACCH"
     gene            complement(19588..19737)
                     /locus_tag="PK522_00885"
     CDS             complement(19588..19737)
                     /locus_tag="PK522_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40317.1"
                     /translation="MDLEYISLTYMQEAQNGNGSNHPGPRSTDEWDRVDVIPWYSFSV
                     TYNFL"
     gene            19966..20409
                     /locus_tag="PK522_00890"
     CDS             19966..20409
                     /locus_tag="PK522_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40318.1"
                     /translation="MADDQNKRPPWRPSEYKPEFCEMLIDHMAQGLSFETFGAVVDVV
                     KATLYRWVDEKPEFRDAKEMGTLKSQAFWEKLGTDNIINKSDSESQGQGYGWSKSRSI
                     NAAIYKLHMANKFGWREKTEVSSDQEKPLTITMNYERKKKPKADE"
     gene            20402..21757
                     /locus_tag="PK522_00895"
     CDS             20402..21757
                     /locus_tag="PK522_00895"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HNC40319.1"
                     /translation="MSELYSKPNFSDFSPRVIPYQSDVVDFLDEWDFSSGTPEILLSG
                     SVGSAKSILMAHLIVRHCLENPGARVCLARKALPDLKDTIFKEVLEHISEDFTEGKHY
                     WVNNSSAKITWWNGSEIISRSWADKKYKKARSLKLSMVVFEELTENNDDDKEAFDTLK
                     ARLRRIPTVKENILIAATNPDSPGHWVHKYFIDSDKRTRKVFYSVTTDNPFLDPVYIE
                     QLKQDYDPKMARRMLYGEWLEINEERVYHSYDPENNFIKGPYQINNHYPIILAFDFNI
                     GAGKPMSSCAKQWDGRSFHIFDEVIVQGARTEEVADEWIEKGIVKQGQRIIIRGDASG
                     DAKDTRSKLSDYDILRKKFAAVGAIVEFQVPRANPPVRRRHNLVNAYCLNDQGAIRLF
                     VYEKAKTCHEGMRLTALKKGGDYIEDDSKPYQHVTTAIGYSVVYQHNLINTNQVYSSG
                     R"
     gene            21761..23230
                     /locus_tag="PK522_00900"
     CDS             21761..23230
                     /locus_tag="PK522_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40320.1"
                     /translation="MLNLLNPSVRKQIIEETQGNENVERKKQSFIQSEVYKDRIYQPV
                     KKYLEPFYSEKTINETPIIASVNLARRIVNKEASLYCREPERKFYGLTDAQIELIKQV
                     YDDMKIDTIMQRINQNYKLENQTHCYIVPRAGKLKMIPMMAYQLDVVPSSADSEIGEV
                     YLVNGFDRIAGNVQVTTTGDSTDEMIADEDDYKKARKAISVWSPMFNFIMDEEGNIIN
                     TEDTSNPIGGVVPFVDVFSSKDSEYWVRIGQSVTDFTIQYNAALTDMSNIVRMQGFGQ
                     AWLKSGPDQKMQNVQIGPNKILHLPTDPNGGQSAEFGYANANPDLSGSLSYIEGLLSS
                     FLTSRGVDPKTVNAKGEAMTYTSGLDRLLAMVEQFEATEADTEVMECAERQILKIVIA
                     YLNTYAGTPVLPNYPSVTLPLDATVDVEFQKPTAIQSDSEKLANIQMRLELGLIDQID
                     AIAEDRNIDREEAQVIFDKMTGVQSQNNQQSQDMTQQDG"
     gene            23223..23798
                     /locus_tag="PK522_00905"
     CDS             23223..23798
                     /locus_tag="PK522_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40321.1"
                     /translation="MAEPGIKLSQDKISQTIDLNELVGKDISSDERLVTRIGQAIIDY
                     MDQRVENGMGLGRQRLKSPYSKEYQDSLEFKAAGKSKGHVNMRLSGDMMAAVDLLEVD
                     GAKITYGIEDSEQAAKAYGHQTGFKGHPTLEGTGNKREFFGITTDELKKYVLKEFEQD
                     IKTSSASNTNQEQGLINAVRSLKTLADFFGG"
     gene            23800..24321
                     /locus_tag="PK522_00910"
     CDS             23800..24321
                     /locus_tag="PK522_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40322.1"
                     /translation="MFKFNGPDEEEFRNRLNKMIKDPVLLNELGDVMIKDIKFTARKG
                     VNPITGGKFIPLSKKWIAEREKISQSTPVNDAFKPARSNVTLSGQLLDALRRSATNGI
                     LSIFFDGIHRPYRAKYSEMRKKQATTRQIGKPIPNNELAQYVNEIRPFFYVREKLLPQ
                     LKTVVIRYIRRNL"
     gene            24359..24937
                     /locus_tag="PK522_00915"
     CDS             24359..24937
                     /locus_tag="PK522_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40323.1"
                     /translation="MSVDNPSVGDATNETSGQPSNKDSVAYDTYRKVLGEAKSLKAKL
                     AEYETKEAEREQSILAEQGKLKEALDLSIKKQKELEANLSAKDKAFAKKVFTKEVESV
                     ALQLGARKEALEDIVKVGDWSSVEIDEDFTINQEQLKASLANLAKSKPFYFVGNPKAP
                     SDVSTAANGAPKGKALNELTKEDILAQLKNLK"
     gene            24959..25810
                     /locus_tag="PK522_00920"
     CDS             24959..25810
                     /locus_tag="PK522_00920"
                     /inference="COORDINATES: protein motif:HMM:NF023081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HNC40324.1"
                     /translation="MAANNLTNTKQDLIAALVQRELKEKASLAPFVMDLSSLAVKGSK
                     SVSVPKLSSFTVQSRTLGSAASPNAALTDAKDTINLDKHKIVLFSYDSHDEQQSTIDY
                     MASAIGRASSAHGRQVNSDILTMWSSVAGLNVNGGTPADITADDILDMREFLIKNFAD
                     MTKTALIIAADQEKAMLKLAEFSRYDYRGGGTAPVVNGQIGFVYGVPVIINQQVAAQQ
                     AFMVNMEGSGYAFQQAPSVAEQSDISYGTGGKLVAVDCDYGVGGLQLGEGTAASGKSP
                     LIAKLAD"
     gene            25894..26052
                     /locus_tag="PK522_00925"
     CDS             25894..26052
                     /locus_tag="PK522_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40325.1"
                     /translation="MLKTNARAGASFQYFDINQFTDHNGKLRWIAWYYSDIGSIGELD
                     GTAEQLQG"
     gene            26323..26583
                     /locus_tag="PK522_00930"
     CDS             26323..26583
                     /locus_tag="PK522_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40326.1"
                     /translation="MRKKFNPFSGTFDFIGTTSSDSDSNCVILLNSVSIESTGDLEIT
                     DEACIEVKNSGVTFAQRINNTVTVNNNGEMIIDANSEVEVSA"
     gene            26583..27161
                     /locus_tag="PK522_00935"
     CDS             26583..27161
                     /locus_tag="PK522_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40327.1"
                     /translation="MSQLKLQIGSVPTTPASGWITIYPKGDKKLYYKDDAGNESVLDI
                     SDRLILNGPGAPASTTGQDGDYYIDNTNTLLYGPKTSGSWGSPVTMVGPQGPTGATGA
                     TGATGATGATGAAGTDGNVWRNGNGTPSNGLGVDDDYYLDDVTSDYYKKVSGAYVLQG
                     NLNSRNTNIDGGHANTIYGGTLPIDGGYSTSF"
     gene            27171..28379
                     /locus_tag="PK522_00940"
     CDS             27171..28379
                     /locus_tag="PK522_00940"
                     /inference="COORDINATES: protein motif:HMM:NF037250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40328.1"
                     /translation="MAQQIQLRRDTSSNWASNNPILAQGELGLETNTGQFKIGDGSTA
                     WNSLSYGGLVGPAQTNPIMGYGSGLDGDVTLSAGITTLTRNMYYNNLTLTGTARINVS
                     GFKIFVKDTLDITVAGPAAIYAIGNDGASATTQTGGAAGAALASTANGGSTAGSAGGT
                     GTAAAGAASAAATVASPANGGASGASGAGGSGSSGAGGASGATVIPTLSLPVNHFQTD
                     LNRGNSLLLGGNGARGGGAGGGDGTNLGRGGGGGGGGGGVLHVFSRKINRSGSTSVGC
                     FSVKGGMGGNGATGSVGNIGGGGGGGGGGGGWAFILYDQLLGTTSTQTIDASGGDGGI
                     GGNGFGTGIGGNGGNGGVAGRITIVNAINRTGFDISVGSGAGTGGTASGVTGGAGGAG
                     DVLKLNLQGV"
     gene            28382..28840
                     /locus_tag="PK522_00945"
     CDS             28382..28840
                     /locus_tag="PK522_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40329.1"
                     /translation="MITRDILDYNGDVIGSMDFPDGTSDEYISSVLSQYAVEPIVDND
                     SYLKFSIIERKKFADELLERFKLRNINEGINALQGMWMHHRLRAYPVSFGGMNFTIDI
                     MNLAISGDLEIACLCLLYGDTDDGTETYHWLTSDRKAYLINELKSFLGWN"
     gene            28840..29073
                     /locus_tag="PK522_00950"
     CDS             28840..29073
                     /locus_tag="PK522_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40330.1"
                     /translation="MKKMTMIILGIVFVCLVLWDVYAMVKSGGSEASISSVIINFAYK
                     MPMFSFSSGFVCGHLFWRMRTNKDTKHIDDQGE"
     gene            29073..29777
                     /locus_tag="PK522_00955"
     CDS             29073..29777
                     /locus_tag="PK522_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40331.1"
                     /translation="MAIFGNIELEDIVQVNDKTRLNCVKSFVSKDEAAISLVRIKPEA
                     SGSFIQVGTTNQKEYYLDWEYSTPGNKTVTLEITTNETAQTFTKNIMVVNAASDKLLS
                     CDANLIAIEPDILKWVPIGRNSFLNVHRKALDLILDWLDSIRVWRDDGTKLTKEDISY
                     TDDLNKLSTYITLELIFMGISNKVDDVFLNKAREYRNKAQEMKNRGRIQADLNGDQEL
                     DKAENVDMRSFDLVRR"
     gene            29774..30205
                     /locus_tag="PK522_00960"
     CDS             29774..30205
                     /locus_tag="PK522_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40332.1"
                     /translation="MSLQSVRPYITARLTALGYKEHVDPFNDENIPASLVDGAFHQLM
                     LPISGVDRNSVAQGLDVPVQVKVLMTGYRNPEEALTQSIANAESIISDITLHSNYVNN
                     APPITGVFLDSLEFGPYSVESNDNIVQAVFVFRFKVYTCVE"
     gene            30227..31156
                     /locus_tag="PK522_00965"
     CDS             30227..31156
                     /locus_tag="PK522_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40333.1"
                     /translation="MACNTQTYNLGIRNILLGSDRPQKTCIFTKADVSSSLQNKYIVM
                     HEPVTQAKHYFWFNVATAGTDPAVPNATGHAVAISSNATKNAVATALQTVIDALAWAS
                     ATVSGNEVEVTMTADGYAYEARDALSSLSKTGFSVNVAQFGSVQFDAGGTNGDITFTI
                     EEQTKEIKSPQTGDFLLAEIRRGSNVTASFELKDSSAESIRRALNFYGQTIVTDDAAS
                     EVITGHGSNNLFKSTDDVADQVILRPTDKAGDADASEDFTIHKARLKLGEITFSAENE
                     LVLPIEVMGYLDTSKSGFANLFSYGNGSALPSA"
     gene            31170..31427
                     /locus_tag="PK522_00970"
     CDS             31170..31427
                     /locus_tag="PK522_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40334.1"
                     /translation="MEFVFEAKEKKMTLNLYGTKYQIRVPKMKESQELQNKIKNADPG
                     DVVQIYMDFFAGIGLPVESLDSLDAIDFQELISFILYPKKS"
     gene            31454..31714
                     /locus_tag="PK522_00975"
     CDS             31454..31714
                     /locus_tag="PK522_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40335.1"
                     /translation="MCRFYGWSDDYVESLDIRTFIDYWNAVTVIEARERLVDLNVAMI
                     PHAEDKDRKKFYKELEKKAYPVRKSTGKKLTNKELMDILSRR"
     gene            31724..33745
                     /locus_tag="PK522_00980"
     CDS             31724..33745
                     /locus_tag="PK522_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40336.1"
                     /translation="MADQKIELEIVLDDGSIKKAFGTIRKEAEETSKETQGMFGAFGN
                     AGVAFVALNQGLELFAKLGDSIKGVAKEVINLSLEAEKVRATQAQFTQFTQQAGIATE
                     QFSKEIEKSIAGLIDDDDALQIANEAMIRLGSTAQRLPQIFELARKAAAAGFGDMASN
                     AEAFTTAIQTGQTKALKQHLGLIVDVTKAQKEYAKEAGIGVDKLNEQQRAVANANAIL
                     DAASKKFNNVDTDVKSFSESIQRLNVAIAEQGEKTAVWFDRVFGSATKGIINTLTDAI
                     NGNGAAVAAQKVKVEDLGKAIEDAEKKIISIQNNLSNARTDTQFSFLNNQLNETNKLL
                     DALRQRQSDSQNFAALGNANDLLSKNIKLSWEKEQADASNSKLAQARAEEQMRIQQAL
                     LQSSQSFQQQEIAARQQSAQLIIDDDKRQEEMAAIHQEQINQIAINGLIARQNIETQF
                     SNEKGFNDAQRAELRRQQIEAENAQIIASQEAFQNQSLNSFQKYSIEQQKKFQQLGSM
                     VKITFINGIGSAFVAFGQALAKGEDAMAAFGKALLGVLGDIAIQMGQMFIWQGLGFSA
                     NPLMPGSGAGLIAAGIGLSILGGVLKGLAGGGGATGSASAGGGVAAGGPESGSFNAAA
                     PVADTRIAPNTVVNFTVQGDILDSDSTQTRIVQLLNDAIDTKGAVVRGL"
     gene            33745..>33925
                     /locus_tag="PK522_00985"
     CDS             33745..>33925
                     /locus_tag="PK522_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40337.1"
                     /translation="MALMTRAKFYFGIEVEQDNNLIDIDEGSGEVSIEIPVGSYSPKQ
                     FSEKLSAVLTSSLTQT"
BASE COUNT        10282 a         6650 c         7140 g         9753 t
ORIGIN      
        1 aagcaattta gcgaaaagct ttctgctgtc ttaactagca gtttaactca aacttatact
       61 gttgtttaca acagaacgac tagaaaaatt actatttcag ctccttctaa tttcagtatc
      121 ttaatcggtt caggatctca taccacaacc acactctatg agaatcttgg atacactgga
      181 ggtattgatt taagcggatt caatagctat gttgctgatt ctacggctgg ttatgaatac
      241 aacccacagt tctatctgct tgattatatt ccactagagc ataatgttaa atctgttcag
      301 gcgtcgatca atgaaactgg atcaggcgcg attgaagtaa ttcgctacgg tacaaaacga
      361 tataccgagt tcagcattga attaattact aacaacaaat tcgtaggtga ttcaatttgg
      421 gttagctcta aaactggagt tgaggacact ttaaacttct taaattatgc gacgcaaaag
      481 agccgcattg agttcatgcc tgatggctat gatcctgcta cttattatac acttatacta
      541 gaatctaccg agtcagatca aaatgggact ggttttaaat taatggaaat gctggaatat
      601 ggtgcaggat actacaaaac aggtaaactg gtttggagag aggtaacgta aaatgtctgt
      661 tgtaaataat caggatgcga cagcagaagt tttcaacgca gcatttgcta gtaagacggc
      721 tgacaatact tctacaggta aacagactct ctcgcgtgtt ggttctggtg catctgttgt
      781 agatgttcaa caagcaatca acgataacat tgcagacatt gcgagtgctc agtctgacat
      841 tgacattcac gttgctgatg ttgcaaatcc tcactctgta actaaagcac aagttggttt
      901 gggcgctgta gataatacat cggatttaga taagccagtt tctacagctc agcaagctgc
      961 gcttgattta aagattgatc tcacagaaaa gggagccgct aatggtgttg ttcctcttaa
     1021 tggttcatcg ttaattgatt ctacatatct accatcatac gttgacgatg ttttagagta
     1081 tgcaaacttt gctgcatttc cggtaactgg tgagactgga aagatttatg tagcactaga
     1141 cacatcaaaa atatatcgtt ggactggttc aatttatgtt gaagtttctc aaggtgtgac
     1201 agatcacacg caactttcat caattggaac caatacacac gcacaaattg acacgcatat
     1261 tgcatcaact tcaaatcctc acagtgtaac aaaaactcaa gttggattga gcaatgttga
     1321 taatcttcaa caacttccga tttcatatct tgatacagat ggaacacttg ctgcaaattc
     1381 agatgttaaa gttgcatcac aaaaggcaac aaagacttat gttgacacac aaattttagc
     1441 taatagtggg agcggaattg taaccgcttt aatttttgga taggatctaa ttatgaaaaa
     1501 gtttatcaca ccagcttata catttactcc aggtgggtct ggtgttggta ctgttgattt
     1561 aagtggaatc agtgggttta atgttaagaa tttagtagct gttatcaacc aaactcgcgg
     1621 ggttgtaatc tatgcgactg gtcaatcatc aactagatat acaaacgtaa caggaacaac
     1681 tgtaactctt ttctacgaca caaccggtca atctggttct gatgttctac aggttatata
     1741 tgaagataat tcaacagacc aacctgtaag cgtttcttca tctgctcttc ctactggagc
     1801 agcaacaagc gcaaaccaaa atactggtaa tacaagtctt agcaatattg atacagcaac
     1861 aactgggatt aacgcaaaac ttaactctaa cttcggtgca tcatctaatg ctgttcgtgt
     1921 tgcggctcaa cttggtaatg cgaccgcgat tgcggatttt gctgcaggta actctaccgc
     1981 tcaaacatta agaacggtta ttgcaacaaa tcaaccggcg attgcaacaa agtctccagt
     2041 aaacgcaagt ggatcaactg taagtgcatc aatttctgct gttacaacag taaccgctcc
     2101 agctaatgcc gtagaggtga ttgttcaagc tgatgacaca aatactgcta acttgagatt
     2161 tgccgtaaat ggtactgcta ctacaacttc aggaatacag cttcagcctg gaagaagtga
     2221 aacacttaaa attggttctg atatttcaat tattccagag tctggtactc agaagtataa
     2281 tattatttgg gtgacacaat gattagacaa ttgattttag cattattaat ttcaacgcaa
     2341 gcatttgcag gattgcctcc aaccacatca aagggcatta gcgatactgc aaaccaaact
     2401 acctttaact ttcagtttcc tcagttagtt ccaactagaa ctggtacaac cacctcgctt
     2461 tctccaccag aaaatggagc attcgttaag aactacataa gaaatggaaa tgctgagagc
     2521 ggtgctacta ctggctgggc tgtttatgat gacggcgctg ctgttcctgt agatggtact
     2581 ggtggtagtc ccgttttgac attggccgca tctaactctg gaccacttgc cgggacttat
     2641 agttacttaa acagcaaagg caatgcgatt tccggctttg gtaatggttt tagctatgat
     2701 ttcacaattg accaatcaga tactgctaag gtcttgcaaa ttcaatatga ctatttgttg
     2761 actggtgcct ataccctagg gtctgattca actactggga catgggttac ttatctttac
     2821 gacgtgacta acgcacagtt gattcagcca acttcttaca agcttctcgg tggtggtcgt
     2881 tttattggta acttccaaac caatagcaat agtacgtctt accgtttaat cattcataat
     2941 tcgcaagttc aacttagccc tgctacgttt aagttcgatt ctgtaattat tacgccaact
     3001 gtttacgctt ttggcactcc gattacggat tggcagagct atacacccac gtttactggt
     3061 tttggaactg cagcgtcaat tgaatgtcaa tggagaagag ttggcagcaa tgttgaacta
     3121 agaaataagt ttacaaccgg aacaccaact ggagtagaag caagagtttc attacctacc
     3181 ggattaacta ttgccagcac ttcaacggtt ccgagtatac aaccacttgg tcaagtttat
     3241 agaggtacga ctggtgctgc caatatatat tacgcccaag ccaccgctgg tcttacatat
     3301 cttacaatag caaaacaaga tagcgttggt ccattcgcta gtacgttggg aacatctttc
     3361 ggttcggcag aggttgttta tcttcaatcg tctgttccaa tccaaggatg gtcttcctct
     3421 gttcagacaa gcgaccaagc ggatagtaga acattgattg aagcatataa taataatgct
     3481 ggaaccgtac taactgcaaa cactacaaac attgatttta gtactaaagt cacagatact
     3541 catggcgctt ggagtgggac tgtttttaca gcaaacgcgc ctggaaacta ttcaataaaa
     3601 ggaagcatat cctgtaccac tggcgcatca agagcatttt atgcttatgt taacggtact
     3661 cagaataaat tggttgcagt aacaacgaca ggcaacgtta ctccttttgt tgctgataca
     3721 tttttaaatg ctaatgatac attctcatta agaagtaacg catcatgtac gcttaatgct
     3781 gttgctacag atcattggat aaacattacc cgctctccat catcatcggt aatttctgct
     3841 actgagactg tggcggcgag ttactatgca agcgcaaacc agacaactgc ggcgcagatt
     3901 aactttgaca ctaaagagta tgatacgcac ggtgccgtca cgaccggagc aggagcatgg
     3961 aagttttccg ctccatattc tggcttgtat tctatcaacg tcaccggata ctcaacaagc
     4021 aataccaacg tatctatttt caaaaacggg tctaactata aatatttgtt ttatacggtc
     4081 ccgtcagaaa acggtagaac gggttcagct caagttagat tgtcggctgg agaatacgtg
     4141 gacattaggc caagcgcatc tgttcaaata aacggcggcg cacaaaacgc tgtggtaaca
     4201 tccagaattg aaatcttgag agtcggaaac taaggagata aaatgctttg tgatttttca
     4261 aatttagaaa gtgattcaag atttgaatta ataagagatg gatacctatt tgctcttagt
     4321 agacacaaaa aagaggtatt gatctattct ttgagaatga attggatttg tgatttacta
     4381 gaaaaatcta aaattggaaa agaaaacgct aagaaaattt acacaaaatc ttacgattat
     4441 cttatcaaga atgattacaa tagattcatt aaatatttcg catttcaaat ttgctcatta
     4501 gcatctgatc tttgcgatga atttaactgt aaagaaaaga tggacgacga ttttaaaaaa
     4561 tttgaatcac ttattgtcgt ttatagtgaa gatattgaag aaataaaagc atatattggg
     4621 ggttaaaatt ggcaacttat agtaacaata caacaattaa aataggttct cctgtagcaa
     4681 ttagaaacac ggggtctggt tctactactt acacagttcc aaacaactgc catttagatt
     4741 taatttatac aaatgttgat ggcgcaggta gtggattgaa tttctatatt aaaagctcgg
     4801 ctaatacgtt tatttataac tatacaggga atacaactca aacacattta tttgaaaata
     4861 gcggacttag acttcctgct ggttcagtta tttatatgga tggatcggtt gcaaaatcag
     4921 tacgtgaaat tattggaaca ttatttatta atacaccatg aagatgatag atgaaaaaac
     4981 tggagttccg atgtttgccg tgatcacagc ggttccgaca ttgtttggtt ttatcatctg
     5041 gttatcgtcg atttcattta ctgctaatag cgcaaaaagc gaagttgcag agcttaaatc
     5101 aaaacaaaac gctcaaaatg aattgttgct caatattaaa gaagacctta cgctaataaa
     5161 gtataaacta gaaattaagg agaagagata atggacgttt taaatcatct tattgaagta
     5221 gttggtaatg cagcaaatgc gccaatcatg ttgagcattg gtattgctgt agagtttgct
     5281 tttcgtatgt tcaagactga aaagccaaaa tctatcattt acatggttgc agatatcatt
     5341 aaaaaatctg gagagttact cactaaggtt ggtactctct tagataaagt attgccacaa
     5401 cgattgaagt aatatgaatc aagtttctgc cattgctagc gcgctcatcg aaatcattac
     5461 cctgggatat aaggtctatg atctgatcca tgaggcgaaa agaaaagggt gggttagtga
     5521 tggcagaagt ttatcacaac aaattgcaga ggctaaaaca gatgaagaac gggctgagct
     5581 ggctaagcgt ctttttaatc acattaaata gctcttgcgc atttggatat ggtcaacgtc
     5641 gttgtgaaag tcctgatatt cctattaggc ccatttctat tatctgtatc agcaatgctt
     5701 ctggtattgg tcagtgttac aaccctgcta ctggaaaaaa tgaacctcgc tcgatggaaa
     5761 atcacgtttg caaagatgtt gcagacttta accgtcaaga agagtggata gagcaagttc
     5821 tggagtcatg caaaaaatga atgatgaagc tccacggtgg ttgcaagtag catctcaaga
     5881 gttaggcatt agcgagatcg tcggactaga tgctaacaag cgcattcttc aatataactc
     5941 atgcacatca ttaaatgcgc aatcggacga ggttccttgg tgcagctcgt ttgttaactg
     6001 gtgcgttaaa caatcaatgt taaagggcac gttttccgct gctgctagat catggctatc
     6061 atggggttat gagtgcgaac ctagatatgg gtgcattgtt attttaaggc gaggtaatag
     6121 cgagactcaa ggccatgttg gatttcttgt tgatcatgat gatttattcg ttcatttgct
     6181 aggtggtaac caaggtaata aagtatctat tcaaaaattc gcaaagcatg aagttattgg
     6241 ataccgatgg ccttttaagg tggtagcata ggcttatggc ttaccagcta tctagcagag
     6301 caaagacgtt attaaatcag acgaacatcg agccgaatat tgttttgtgt atcggtgact
     6361 atggatgctt tggtgcttcg attactaagg ttaaagttaa gattggaatg ccaggcctta
     6421 agatcggtgg cggctggaag atcggcggca cgattcagga tgagaacatt gtcgattata
     6481 tttccttgga tggcacaact actgacataa cgcaacaatt agaccttgat aaaggtgggg
     6541 ccactagcac tcaaacggtg gacgttcgcc tggtagacat caatggcgag attacccgtt
     6601 taattagccc aggattcgat ttaaacgaca ttctctatac agacgcgcaa ttatacctag
     6661 gatttaaaga cggcgcattt ccgcaagatt acgttgatct attcattggg aaggtgcaga
     6721 gcattaaaag cggatctggg tatattgatt taaccattgc ccaccctgat gagctgaagc
     6781 gcgcagagat attcccaaag tttgagacat cgcttgttgc agatgcgcct tactattcaa
     6841 aatcgattca agacctaaca tattatcaat cgggtaacta taacggcatt gtgcaagttc
     6901 gctacttaaa cattcctttt attggtgacg ttgcaaacat tactgtttct ggtggattga
     6961 tcacggcaag tattgagtca ggcgttacta aggccaatac aattaagaat gcaatccgcg
     7021 atcatttcga ggctaataac ttagtgcagg tgatggtgtc cggcactggt acaaatgcgc
     7081 agacaacgca agctacaaca agtttagaag tatctgatga gctattccta gagtcagtcg
     7141 atggtctata tcttccgcat ggggacatat tcaaaacata cgttagaatt gatgatgaaa
     7201 tcatagaata cactggaatt gatacaacac ttaaaaagct tactggatgt gttcgccgat
     7261 ctcttaattc tctgggcaag tctcataaga tcgacgatgc ggtacaatca ttctataagc
     7321 taggtgataa tacagatgcg tcaaatgcga ttgatctagc attaatgcta atgctatctg
     7381 atggcattgg ttctccatat ctaactggga ttgaagacct acaattttat aacttcggaa
     7441 cattaacaaa ttatcctaac ggattgctaa ttacaggagt agacgctgta agacgatata
     7501 atattcgcgc tggtgatttg ttcacagttt caggcgcatc taatccagcc aataacttca
     7561 cagatcaagt gattgaagcc gttgatgtga ttgaccttgg aactattgtt tatatcgctg
     7621 ggcaatcgtt cgtatctgag ccaatgagtg ctgctcttgg aagtgttaag tctcaatata
     7681 acgttctacc tgacggttgc ggattcattc ctaaccaggt agacattgat agattcaagc
     7741 aaattcaatc tcgattccct tcatcaatcg ctaactatga gctttatttg aaggatacag
     7801 ttaagccgaa agacttaatt aatactgatc tattcttacc ttctgcgctc tactcaatcc
     7861 ctcgccaggg taaggcatct attgggatta gcgcaccacc actttacgag ggcgggacta
     7921 aaacgcttga tataaccacg gttaagaatc catctaaact gaagatgacc agaactgtgt
     7981 ctaagtattt ctacaactct attatttata aatacaatga ggactcagta gatgaccgcg
     8041 ctctgtctgg tttgatcatt tactcagccg attctgttaa tcgtattgca tcaccaaata
     8101 ggccttacac cattgaagct aaaggcgtta gaccatcgcc aactaatact caattaatca
     8161 ctagaaactg taagcggtat cttcaacggt tccaattcgc agcggagcaa ataccagtag
     8221 agcctgatta taaaactgga tacacgattg aagtaggtga ttctgtcgta ttcggtgact
     8281 cagaacttca agtaagcgac tcagtaagtg gaactcgtga ttttaaacct cgcgttttcg
     8341 aggtagttaa taaatcattc aattggaaaa ctggtaagat tactttaaac attgttgata
     8401 ctaactactc ggcaagcgtt cgctatggtg tttggtctcc tgcatcgatg gttaaggctg
     8461 gatcaactac aactaacatc aaggttttaa actcatacgg gtcaacatca gagcgcgaca
     8521 agtggactca atactttgga cgtaccgttc gagttagatc gaaagattac tctatatcac
     8581 aaaacgtcaa gctggttgga tttgatccta catatgatga cacaatgatt gttgatacgt
     8641 taggattcac tccattggat ggatatattg tcgatactcc gatttacaat gatcttaacc
     8701 ttgcaggtga ttcattctat aaagcagttc atccgttctg gacgcctaca ttgaccgtag
     8761 tcagtggaac tagctcaact gagtttgaag tatctgttgg agacgctgca aaattatttg
     8821 aaggctctac tattcgcatt cataatgcag attatactgt ggatagtgca gagaaaacga
     8881 ttaaggttag ttctgtagtt ggtactactg tgacctgtga tgacattggt tttactccga
     8941 gtgcggggca attaattaac ttggtagggt ttgtctcaga tgatggcaat ccttatgtat
     9001 gggtgtgagg gactatggca actgaagtaa cacaaattaa acagaaaatt tacgaggaag
     9061 acgtagaaac aggagcggct gcatctgaag agaccatgac caaatttgga gggtctttga
     9121 acttgctact tgataagaca atcttccctg tgcatttcga tattcatggg ccatataaca
     9181 tcactggagt gcctgatgca aaggtagata aattctattg cgttccattt gatgcagaga
     9241 ttgtcggtag ccatttctac actggagaaa atcctggttc atccggatca actgaaattg
     9301 atattttgcg caagcctcta tctggaggaa cggcaagcat tttctctact cgcccgataa
     9361 ttccagcaag tgctggagcc gaggcagata ttatccaaac ttatcttcca tcagcatcta
     9421 ctattagagg cgctactggt gggactgatg ctgttctttc gtctactcaa ctagatgcgt
     9481 atgatgtatt gaagtttagc ttcattgata agccggttgg aactgtagat aagtgcggat
     9541 tcgttttatg gttacgcgct agataatggc gcattagctt aacggtaaag ctgctcacta
     9601 gtgacgacta tttgagaaat cctgttcgac tcaggatgcg ccgccattat tttccaccct
     9661 tcataaagct atcaaactca ctattttcgc tctccaatga gttaacgaat gcggtagcct
     9721 ctgatacaaa cttttgcatt tgctccattg taaaacctga gtacggactc ttcttcgcaa
     9781 ttctatcttc aaagctaatg atagtatcct ctagcttacg ctggccgata atgtcgataa
     9841 cctgcggaag tgtgcggccc ttgtgagtgc ctgatggtat tctgtaaata ccatcggtag
     9901 aaaagccagt ttgcgggtct tctgctgttg gttgatctgg gatgattttc ttttgtgatt
     9961 cgtctttctc tggaaggtct tcacccgcat aaatataaag accaatcccg aagcaagcaa
    10021 tgcacttaac aaggcaacgc atcattgaat tactaatctg acaggcgtta gggtttttaa
    10081 tggcttgatt cttataatcg aatacagcaa ggtgcatagt cattgatttg ccgaatgcat
    10141 ttactttaca ttgaaccata acggtatcat taatgttcat gtgcggaaga aattcccaag
    10201 tcgcagttgg atcagcttga agcaattcat ctactgcgta tgtccatgat agatagctaa
    10261 tgccattttt cttttcaatg tgttcgctta cgtttaattt tcttaactcg ttaaatgtct
    10321 tcatttcttc ctcactatct taatttttct tcccattgca tccagcaact taataaagtc
    10381 gtcaaccata acgcctttgc tatcgtgttc aaccttgctg atatttgatt ggcttacgcc
    10441 gctgcgcttg gatagctcaa cttgagtcat atttgcaagc tttctaagca taaaaatata
    10501 ttgtcccggg gtgtgattca tttcttcacc actctttcaa acttccatcc tttttcatca
    10561 agaaagtcta aataatcgtc atctgtaaat gtgtagtcga tccattcttt aatgccatca
    10621 cagccacgtt ttgaatcgcc gtctgcttct ttgtatgctt ggcaaagctc tttgtcacat
    10681 tcgtcataga accagtctct aaactcttta taagagccat actcttcacg aatattgccc
    10741 tttgatacaa cgaaagtaat ttcagggatt ttaggtaact cgttattgtt ttgcatgaat
    10801 aaagaatatc gcaaaacgaa tatttttact aattagtagt tctaatggtg agctatcccc
    10861 actgattagc catagcgtct gcaatccctt ggaatgtagt agagcgtatt ttccatctat
    10921 ctttagatgg tggaagttta tgcaaccgct gctctcgccc ttcaacaata tttgtaggcc
    10981 ttaacaaagg aaggttttta agccataagc aagtggcttt agtttcaccg tgaccaaact
    11041 gccatggttg aattatttgc gattgattga cacatataat ttctttcgca tatttgtgca
    11101 tgataggatt ttcaactgcg atttttggaa tagaacaatt aagtaaacgc ttaaaaaagt
    11161 ttgctcctaa tcgcatttta tcccacctag attcgtcttt gtgtagccag catacaccac
    11221 tgttgcatag gtaagtacat ggagggtgtg cgatcattaa atcaaagcca tcattaataa
    11281 tatcaaaaac gtctccttga tagtgctgcc ctggtttatc ggttggcaat aaatcgcaac
    11341 taatagcatt atgaccacgt tttttaaaag catctctaac aactccagaa tattcgcaag
    11401 caactaaaac tttcatgggt ctcttataca ataactgcgc attggttaaa tattagtagt
    11461 tctaatgatc gactatcccc attgtgacgc cattgcgtcc gcaatgcctt ggaatgtttt
    11521 actccgctct ttttgtgatg ctttagcgta ccactttgaa ttggatgtag ttccatcttt
    11581 tcgcgtatat cgctcgcctt ttgaaactat ttttgttggc atcaacagcg gcaatccctt
    11641 aatccataga cacgttcttt ttgaaaactc ttgaccgaat tgccaaggat taattatttg
    11701 agaatatttt ggaagttctg catatctatg gggcaatgga ttttctacac atagtttttt
    11761 tgtatttgca tttaaacaca ataaaaaaaa ctctctagca gacaacattt ttccccacct
    11821 atcattttgt ttatgtagat aatgtgctcc tggtacgcat aaataagtac atggtgggtg
    11881 tgcaataatt aaatcccatc cagcatttaa tattgtacta gcatctcctt gaatatgatt
    11941 tcctggtatt tcagtaggta gtaaatcaca tgaccatgca tcatggccgc gccttctaaa
    12001 tgcttctcta acagtaccgc tgaactcaca agcaactaaa actttcatta tagtgttata
    12061 ttataaatgc cattatatta aatattacta tttctaatat gatgactaat ttagactgtc
    12121 tttttctgat acgttgatta ggaatgtctt gccgtggata ttgatcataa ctaaaccatc
    12181 gagcatatgt acggctttaa atcgttgtcc ttcaggcgat tgcatttgag aaaagatatc
    12241 cttgatgatc tcgctaatat cggactcacc gtaagctgta atggcctttg ctttcttcac
    12301 tttaaaaacg ccttcctagt gttttgatga atttcattaa tcttagattg ggagatggtg
    12361 ccgcagttct tacagcgata agtttggaaa gcgccagtct ttgagtagtt gaacttatcc
    12421 tcgaataaat cctcgctgcc acaattaggg caacatccag ccttttgagt gtaggtacca
    12481 aagttaatag tgtcgtccca atggcgaagg ctatttagat atagctcctc aagagagaga
    12541 acatcataga tattgtattc cttcatttca gcccatgctt ctgggttacg gttgaggcat
    12601 tcaatccata gcttcatgcc agcaaacttg ccgtggtcta gcttcttgta ggtctcattg
    12661 aagtaatcgg ttgagtaagc gagcttgtta gacgttaacc caaagtgctt tttcttaatc
    12721 ttaagcgtat caatgtgacg gtaaggggat gggcggccta gcttgtactt ttcaaacttg
    12781 gtattaagtt tcttctcatc gaacttttta ccgttctgag taatgatcac atcggcctca
    12841 tccagtttgt gccacatgag tcttacaatg cgctcatcgt tcgagtagtc gccctcgcct
    12901 gatacatcct cgtacatcac gttactctca ccaatccact tagcggccca tgacataatt
    12961 gaccagtctt gtataatttg atctagtcct atattttgat cgaacagacc ccacacatag
    13021 gctattattg gttttaattc taagtctaag acaaggatac gaggttgccg tggttgtggg
    13081 ccttcaaact cttcaatggc ctctaacagc ccagcagcgc gaagtaggcc atcgtgggaa
    13141 ccgaatagct cagttacatt gattctaggt agcttgtttt taaaatcggc tgaggtgggg
    13201 actcggttaa gttcagctct taactcttta atgatcttaa cgcagtaatc ctggcgctcc
    13261 tttagactca ttaatacccc cttgtgataa gggtgaatca ttccagcatg aatgcaatca
    13321 tttaatgaga gatagaagcc tatgggagtc cttaataatc tgctcgccat gctttgcgaa
    13381 tagctcacaa aaccattctg tcgtttggtc tcgatctagg gataattcat aaaaggataa
    13441 ttcccattga tagcaatgca cgatttcgtg cgttatggtg atgatatcaa aggaagattt
    13501 tctaaagtgt atttttctag cattgagatg agctatagca tggcactttt taaacttctt
    13561 gttgtgtact ctgtcgcttc ttacgaatat gtcccagttt ttccctagca ggtttatctt
    13621 tgttctttgc atccttttta ggatgaatca catcaatgat cacttcaagg aagttgttat
    13681 ggtcacacca ttgctcggcg cattgctttg gtttccttgg atataatcgc acccgctcca
    13741 ttcggccctg tgcaatatca atctggttat gctcgcaaag ccaatatttg aatttcattt
    13801 taaaataact tcagagcatt tctcgcaatt agtcacattt ccagcattgt ccatattgca
    13861 accttgcccc tcatcttccg caccacactt ttgacaaggc caatgaatag gggggaaatc
    13921 gggaagtgtg tccaatattg ttttattgtc aaaaaacata attgaaaact cgacccaacc
    13981 aaacataatg gaaaaacaat aatgcggatt aaaaatgatc cctaatataa attgtggcgt
    14041 attttgtaat tttattttca taacttaatt ttaactctca ctttggcgct ctgggcgttg
    14101 ctgtaaagtt aaatattgtc ggccctcttc ttcaaaccat tattcatggt gcattcttag
    14161 gccgcaatag ccgcatgatg catactcatc ctcatcgaat acggcgagaa ctttgccatc
    14221 aatatttctt tttttgcatg aattacaacc gcagcgccat tcaccgccac attttggaca
    14281 tttcattccc cacccctcaa agctttgatt ttttcgagcg attcttcaaa tctatgcttc
    14341 attaaattaa gtgcattggc ctcatgtgcg tgttcaaccg atattttacc atgatataac
    14401 cacaagtctt gcaatctcag tgctgcctct attacctcca ccgcagcgtc gagagcctga
    14461 gtggttgcgg tgagttgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntca
    14581 ttccttcacc cccaaaaact tgcgctcgat tttcgctgcc caaaatttgc atgacgtatg
    14641 aactaggcta ttttctgttg atatggcctc atcacttttc agaaactcac acacgcgcct
    14701 cacagtttcc tcgtctatcc cttggctgtt tagagaacgg acgtggatca agctatatat
    14761 ccattgaggc ggcccttgca ttactggttg cgaaaatgca tcaaaacagt ttggcatctc
    14821 gtcatcacac ggtattggat caatccaaaa ctccctcggt tttctttggt ctttcacttg
    14881 ctcacacttt ccgctctcgc ttcgtcgatc ctctgtttgt gggtcataaa tcattccatt
    14941 cttttacaaa tccaatgcaa gctttttttc cgagtaaaag cggccaaaat attgcccatg
    15001 caaggcacac ttctgctcct tcatcaatca tggcaaaaaa cataaacgcg ctaataacca
    15061 tatataaaaa aatcataaaa ccaatcataa actcccactc tctttcatag ccgctcgaat
    15121 cgcatccctt ggcgtttcat attcattgtc aaaatctaaa ttgataatcc acttgtcatt
    15181 aatctctaat aaataggtgt tatttatatt tttaattaaa aaatcaatca tcatcgaatc
    15241 agtaatcacc gactctttca ttgctgcatc atatccggct ttaaatgcta tctctaagtc
    15301 atcatcatta agtggcccaa agtctccgat gcatccgcta taataactca aaagccatcg
    15361 tctaaatttt ccagataagt ctggatattc tgccttttta tttgtcaccg gctcactcgg
    15421 cttgatttct tccgttgtat tcatgaaaaa tccatctgac attttcagca tattagccct
    15481 tttgattcat ccaataaata attcgacatg aaacatattg ggaaggaagt aaataacccc
    15541 atctcgtatc gatgctgcat tcctctggat ttttacaatc cattgctcgg atagtgccgc
    15601 caaaaattcc aaaaataatc gttaatataa aaagcatact gagtaattct ctacaatttg
    15661 acttcatatc tctccaaatc accccagcgg ttgaaaccta atcagtctta attaattaga
    15721 ccgctggggc ttctctctgc accattgcag aaaattattt atggcctgta cctttacaat
    15781 aagcacatga gttgcctgga tattgcccca tgccagtgcc atcgcaaaac gtgcaataac
    15841 tcgcaaaaat atacatatta aaaaggcgcg tcctgttgct ctttcttact gtccagcaat
    15901 cggaaatcgt taacgatgac ctcagtcgag taatgagtga tgccatcctt ctcatagctc
    15961 ctcgtctgaa tcttaccctc aacgtaaatc ttagagcctt tatcggtgta ctcgccaatg
    16021 atctctgctt gctttccaaa tgccactact cgatgccatt cagtcttctc ttgcttgttg
    16081 ccttgcttgt ctgagaattt ctcagtagtg gcgatagaga tatttgcaat ggcttgacca
    16141 tttgctgtgt gtttcacttc gggcttttgc cccattcgac caagtatgaa tacgcgatta
    16201 accataactt cctttcaatt ccccgataaa accactcaca attccatcgg ggataactac
    16261 gatatatagc tatgggaata cttaatcata tttcttacgc aattagtaat tagtagaagt
    16321 ttataattaa atctggctgt attagttttg cttataatga aagcggctcg gttagacgct
    16381 tgaaatctat gccgagccgt aataggtttg tttccatcta caaaaacaaa tgatgggtcg
    16441 atattaccca tctttggatg aattgcaaca ttgaaggggt ggggaatgat cacaattaaa
    16501 tttaataact gggacaaata taacaagcgg caaaaggata taaaaaaccc tttttggttt
    16561 tcgatgagta ataatttttt ctatgaccct gagttttacg atttctcagc cgaggaaaaa
    16621 tgtacctttt tttatcttct tgcagaggca tctaggaacg gttgttatgg cgaatttaat
    16681 acaaccgaaa cgcattactc aagaattacc ggatatagta aacagacttt atataagaca
    16741 ttggataagc tattgaaaat aaaggtcgcg gcggtttcgc ggcaagatgg cggcgtcaat
    16801 cctgcctcta cagaacagaa caagacagaa caagacaata taaaatatat gtccgatttt
    16861 gaaaaatcag acgcattcga ttttgaagaa atttataagg cgtaccctcg aaggcttggt
    16921 gatatgaaaa agggtggtgg cattaataaa cttaaacggt tgattaaaac acaaacagat
    16981 tttgatgatg cattaaatgc tgttaagcgt tacaatcaat attgtatcga gacaaaaaaa
    17041 gttaagaccg agctggtgaa aatgtttagc tcattctttg atgccaatgg tgattggcgg
    17101 gagtggatta ccttcacagc agataaacct aaaagcagca tggacgcatg gctggagaaa
    17161 tacaaagatg aagcgtagcg agtttgcaga acaaatggga aggcttattg atgtgtatgg
    17221 ggaaaagtca tacccttcac caagaaccga tttaatttat aattgggcaa gcaagatgca
    17281 ggttgaatca ttttcgcaaa ttgtttcaaa gctcattgca gaatgcgata gaccaccgtt
    17341 attgaataaa ttcaaagatg cgcaccaaga attaggcatt ctaaaaaata ggccgattgt
    17401 tgattgcgtt tactgtggcg gtactggttt catttgcgac gatgctccac ttccaaccgt
    17461 taagcgctgc atttgtgaag ctggcgacga gaagacaaaa tacattgcaa ggtttaaggg
    17521 gtttccagtt gccgcatttc catcacaagc aaagcttgca gagattcgac aagctgcgag
    17581 cgatagagcc gcaaagctaa ttaaacaaac gatgttaaat aaaacaattc ctaatgacaa
    17641 tcaacaagac tagataagta acactaatta gctcccatct aaattgtatg attgagtgtg
    17701 ggtatgaagt tcaacaatgc accagtagac cctctcaaga ttcaacttat ccacgatatg
    17761 ctaaatagcc ctgaagtgaa tgcgctatgg ctagagttcg tacaccttgg aaatgtgccg
    17821 caggtgaagc atgagctaag agcgattgcg tttaatcggt atgctaaggc ccgtgacgca
    17881 tttcttggtt taccagcatt gaatgcaagt gcccttgaag agaagtttat taatttaaaa
    17941 taggtgatta tgaaaacaaa tccgaatgat ctagcatacc cattaccaaa ccataatttt
    18001 accgataaga acagggaaca atatatcgca agactggccc caggattaac aaagcgagaa
    18061 tacttcgcag caatggcaat gcagggctta aatgctaatg gtgagctagt aaaagcatta
    18121 gctataaaag gtgacgttga ttatccagga gagtttattg ctacaatggc ttgtaattct
    18181 gccgatgcgc taattgatga attaaacaag gagaaataaa atggcgccgc ttaaaaaagg
    18241 ttattcagct aaaactgtaa gtaaaaatat taaaactgaa atgagtcacg gaaaaagtca
    18301 aaagcaagct gttgcgattg cattgagtga agctgagaaa gctaaaaaga aaaagaagaa
    18361 aaagaaatga aagccatctt ctttgcaatc ttagtaatcg tttgcgtatt ctactacaag
    18421 ttcacacttg atagacctgc aaacgcttgc gccgattcgt atttgattaa tgaaactgga
    18481 aagaaatgca ttactgattg gcgggttgat gagtaagcag aagcaacttc ctgaatctgt
    18541 aattgaatcg cagatatttc attatcttcg aggccgtgga gttctttgct ttaaatctat
    18601 ccaggctggt ttttttgatc ccgtccgtaa ggtgttccgc aagcaagctt ctccatactt
    18661 ccgcaaaggt gtgagcgatt gtcttggatt gcttcctgat ggccgctttc tcgccattga
    18721 gatcaaggct agatatggga aggtaagtcc agagcagcag cgattcattg atgatgttaa
    18781 taacagcggt ggggttgcat tcgtggcgcg ttctgttcaa gatgttgtag ataaattaag
    18841 ttcaatcatt ggatcaagta ccccaatgaa gtgaaagcaa gccctgggca tttcctaacc
    18901 cccgtttggg agtgtcgagg gcttgaattg aaaggcaaga atgagaaaga ttaaacattg
    18961 gttagcagca gcaggattag caatagcgtc attattcgga tatcatcaca ctatccctga
    19021 gactcgcgtc attgaagtta tcccccagcc agcgacgaca ccaacaccaa tcgcaacgcc
    19081 aattgtcgag gaaattcatc ctaaaatcga ttctaagcgc atcaacattt catgcgactc
    19141 aacgtgtacg catcaagaga aaatcgatct agctcgaatt gtggcgaaac tagaggcagt
    19201 gcaatcaagt aagtgcgtta ccgattactt aaaagctcct gacacagcgc ttgatgtgaa
    19261 gcaattggat ggaatgacgc ttgatgcagc gattcagaag gttctaacta gcaaggtatc
    19321 aactcaacta acgtattact atgagggccg taactggttc actaagacgc tcgtaatcgg
    19381 atatgagaac ggtgatggca aggttcacgc taaccgcgca gcttgggatt acatgagcga
    19441 ttgcgagaag gctggcaact tagctcatga gatttctcac cagctaggat ttcatcatga
    19501 ctttgagcgt accgctagac gcccttactc gtttccgtat gtgatcgggt cggctgttga
    19561 ggcgtgttgc cactaaaact atcttgatta taagaaatta taagtcacac taaaactgta
    19621 ccaagggatt acgtcgactc gatcccattc atcggtgctc ctcggcccag ggtggttgct
    19681 tccgtttcca ttctgggctt cttgcatata agttaaacta atgtattcta gatccatgcc
    19741 atctcagtga gtaatggctt atctatcttc agtgaggagg gtacttgtca gattcaattc
    19801 aatttcaagc agtattcaat aaagctcaga ctaccataga cggcggtctt agagtttctt
    19861 tagacctaga tacatcacaa gctcaagcaa tggttgacct aatacgactt cagggacgca
    19921 ccttagaggt tgctattatc ccacatccac agaaagaaga tacccgtggc agacgaccag
    19981 aataaacgcc caccgtggag accctcagaa tataaaccag agttttgtga aatgcttatt
    20041 gatcacatgg cgcaaggtct tagctttgaa acatttggag cggttgtcga tgtagtgaaa
    20101 gcaactctct atagatgggt ggatgaaaag ccagagttta gagacgctaa agagatgggc
    20161 acccttaaat cacaggcatt ttgggaaaaa ttaggaactg ataatatcat taataaatct
    20221 gattcagaat ctcaagggca aggttatggc tggagtaaat cgcgctctat caatgcagca
    20281 atttataaac tccacatggc taataaattt gggtggagag agaagacaga agtttcatct
    20341 gatcaagaga agccgctaac aatcacaatg aattacgagc gcaagaagaa gccgaaggct
    20401 gatgagtgag ctatactcta agccgaattt ttctgatttt agccctcggg ttattcctta
    20461 tcaaagcgac gttgtagact tcctagatga atgggatttc tctagtggta cccctgaaat
    20521 tcttttatct ggcagcgtag gatctgcgaa gtcaattcta atggcccatc ttatcgtgag
    20581 gcattgtctt gagaatccag gtgctcgggt gtgtcttgct cgtaaagcat tgccagatct
    20641 taaagatact atcttcaaag aagttctaga acacatcagc gaagacttca cagagggtaa
    20701 gcattactgg gttaacaact ccagcgctaa gattacttgg tggaacggaa gcgagatcat
    20761 ttccagatca tgggctgata agaagtacaa aaaagcgcgt tcgttaaagc tttcaatggt
    20821 tgtatttgag gagcttacag agaacaatga tgacgacaag gaagcattcg acacactaaa
    20881 ggcccgtctt cgtcgtatcc ccaccgttaa agagaacatt ttaatagcag caaccaaccc
    20941 agactctcca gggcattggg tgcataagta tttcattgat tcagataagc gcactcgcaa
    21001 agtattttac tcagtcacta cagacaaccc atttcttgat cctgtttata tcgagcagct
    21061 taagcaagac tatgatccta aaatggcccg tcgaatgctc tatggtgagt ggttagagat
    21121 taatgaagag cgagtctatc actcatatga tccagaaaat aatttcatca aaggcccata
    21181 tcaaataaac aatcattacc cgattattct agcgttcgac ttcaacatcg gagcaggaaa
    21241 gccaatgagc tcatgcgcta aacaatggga tggtcgatca ttccacatat tcgatgaagt
    21301 aattgttcaa ggtgctcgaa ctgaagaagt ggctgatgag tggattgaaa aaggcattgt
    21361 caagcaaggt caacgcatca tcattcgagg cgatgcttca ggagatgcta aggacacgcg
    21421 ctcaaaacta tctgactacg atattctccg taagaagttc gctgcggttg gggcaattgt
    21481 tgaatttcaa gttccacgcg ctaatcctcc agttcgtcgt cgacataacc tagttaatgc
    21541 gtactgcttg aacgaccagg gagcaattag actattcgtt tacgagaagg ccaagacttg
    21601 tcacgagggg atgcgactca ccgcgcttaa gaaaggtggg gactacattg aggacgattc
    21661 caagccttat cagcacgtca ctacggccat cggctatagt gttgtatatc agcacaattt
    21721 aattaatact aaccaagtat acagctcagg gagatagaga atgctaaacc ttttaaatcc
    21781 aagtgtccga aagcagatca ttgaagaaac tcaaggtaat gaaaacgtag agcgtaagaa
    21841 gcaatcgttt atccaaagcg aagtctacaa agaccgtata tatcaaccag ttaagaaata
    21901 cttagagcca ttctattctg agaagacaat taacgagacg cctatcatcg catccgttaa
    21961 ccttgcccgt cgtatcgtga acaaagaggc ttcattgtat tgccgtgagc ctgagcgcaa
    22021 gttctatgga ctcactgatg cacaaattga acttatcaag caagtctacg acgacatgaa
    22081 gattgatacc atcatgcagc ggatcaatca aaactataaa ctagagaatc aaactcactg
    22141 ctacatcgtc cctagagctg ggaagttgaa aatgattcct atgatggcat atcaactcga
    22201 tgtagttcca tcgtctgctg attcagagat tggtgaagtg tatctagtga atggcttcga
    22261 ccgtatcgct ggcaacgtgc aagtgaccac tactggtgac tcaactgatg agatgattgc
    22321 cgacgaggat gattacaaaa aggctcgcaa ggccatctct gtttggtctc caatgttcaa
    22381 cttcatcatg gatgaagagg gcaatattat taatacagaa gacacatcta accctatcgg
    22441 cggtgttgtt cctttcgttg atgtattctc tagcaaagat tcagagtatt gggttcgtat
    22501 cggtcaatca gttactgact tcacaattca atacaatgca gctcttaccg acatgagtaa
    22561 catcgtgcgt atgcagggat ttggtcaagc atggcttaag tcaggcccag atcagaagat
    22621 gcaaaatgtg cagattgggc cgaataagat tttacatctt ccaactgatc caaatggtgg
    22681 tcaatcggct gagttcggat atgcaaacgc taaccctgat ctaagcggtt cacttagcta
    22741 tattgagggt ttgctctcta gctttttaac ttctcgtggt gttgatccta agacagtcaa
    22801 cgctaagggc gaggcaatga cctatacttc aggactagat cgattgcttg caatggtaga
    22861 gcagtttgaa gctaccgaag cggatactga agttatggaa tgcgccgaga gacagattct
    22921 taaaatagtt atcgcatatc ttaacactta tgcagggact ccagtattgc caaactatcc
    22981 aagcgttact cttccacttg atgcgacagt agacgttgaa tttcaaaaac ctacagctat
    23041 tcaatccgac tccgagaagc ttgccaatat tcaaatgcgc ctagagcttg gcctaattga
    23101 tcaaattgat gccattgcag aagatagaaa tattgaccgt gaagaggcac aagtgatctt
    23161 tgataaaatg acaggggtgc aatctcaaaa taatcaacaa agccaggata tgactcaaca
    23221 agatggctga accagggatt aaactctctc aagataagat ttctcaaacc atcgatctaa
    23281 acgaattggt tggtaaggat atctctagtg atgagcgttt agtaactcgc attggtcagg
    23341 ctattattga ttacatggat cagcgagtag agaacggaat gggccttggt agacagcgtc
    23401 ttaagtcacc atactctaaa gagtatcaag atagcctaga gttcaaagct gctggcaagt
    23461 cgaagggtca cgttaatatg cgtctatctg gcgacatgat ggcggcagtt gatttgctag
    23521 aagttgatgg ggcaaagatc acttatggaa ttgaagacag tgaacaggct gctaaagctt
    23581 atgggcatca aacaggattc aaaggacacc caacacttga aggaactgga aataaacgtg
    23641 agttctttgg aatcacaaca gacgagctta agaagtacgt cctcaaagag ttcgagcaag
    23701 atatcaagac aagctctgca tctaacacta atcaagagca aggactaatc aacgcagtaa
    23761 gatcattgaa gacattggcc gatttctttg gaggttaata tgtttaagtt taatggtcct
    23821 gatgaagaag agtttagaaa tagactcaac aaaatgatta aagatccagt gttacttaat
    23881 gagcttggtg atgtgatgat taaagatatc aagttcactg ctcgcaaggg tgttaatccg
    23941 attactggcg gtaagttcat cccactatct aagaagtgga ttgctgagcg tgagaaaatc
    24001 tctcaatcaa caccagttaa cgatgcgttt aaaccagcta gatcaaacgt aacattaagt
    24061 ggacaactcc tagatgcgct aagaagatca gcaacaaatg gaatcctcag cattttcttt
    24121 gacggcattc atcgcccata tcgggctaaa tattcagaga tgcgaaagaa gcaagcaacc
    24181 actagacaga tcggtaaacc tattccgaac aatgaacttg cgcagtatgt taacgagatt
    24241 agaccattct tctatgtgcg agaaaagcta ttgccacaac ttaaaacggt tgtaattcgt
    24301 tacatacggc gtaacctgta aacaataaca cttgaaaaat gacaaggaga actaaataat
    24361 gagtgtagat aatccaagcg tcggtgacgc aactaacgag accagtggtc aacctagtaa
    24421 caaggattct gtggcgtatg atacttacag aaaagtttta ggtgaggcta aatcacttaa
    24481 ggctaagtta gcagaatacg aaacaaaaga agctgaaaga gagcaaagca ttctagcaga
    24541 gcaagggaag ctcaaagaag ctctagacct ttcgattaaa aaacaaaagg aactagaagc
    24601 aaatttaagc gccaaggata aggcatttgc taagaaggtt ttcaccaagg aggtggagtc
    24661 tgtggctcta cagttaggtg ctcgaaaaga agctttagaa gatattgtta aggttgggga
    24721 ttggtctagc gttgaaattg atgaagattt tacaatcaat caagagcaac ttaaagcctc
    24781 tttagccaat cttgcaaaat caaaaccgtt ttactttgtt ggtaatccaa aagctccaag
    24841 cgatgtttcg actgcggcaa atggtgcacc aaagggtaag gcactgaacg agctaaccaa
    24901 ggaagatatt ttagctcaac tcaaaaactt aaaatagttt caagaaaggg actaaaacat
    24961 ggcagcaaat aacctgacca atacaaaaca agacttgatt gcagctctcg ttcagcgtga
    25021 gttgaaagaa aaagcgtcac tcgcaccgtt cgtaatggat ctctctagcc tcgcggttaa
    25081 aggttctaag agcgtatctg taccaaagct ttccagcttc actgtacagt cccgcactct
    25141 cggaagtgca gcatctccca acgcagcgtt gactgatgct aaagacacaa tcaacctcga
    25201 taaacataaa atcgtgttgt tctcttacga cagccacgat gagcaacaaa gcaccatcga
    25261 ctatatggca tctgctattg gtcgcgcttc ttctgctcac ggacgtcaag ttaactccga
    25321 catcttgact atgtggtctt ctgtagctgg tctcaatgtt aacggaggaa ctccggcaga
    25381 tatcactgct gacgatattc tcgatatgcg cgagttcctc attaagaact tcgcagatat
    25441 gactaaaact gcattgatta ttgctgctga ccaagaaaaa gcgatgctca aattggccga
    25501 gttctctcgc tatgactatc gtggcggcgg aactgctcca gtagtaaatg gacaaatcgg
    25561 ttttgtttac ggagtaccag taatcatcaa ccaacaagtt gcagcacaac aagctttcat
    25621 ggttaacatg gaaggttctg gttatgcatt ccaacaagct ccatctgttg cagagcaatc
    25681 tgacatcagt tatggaactg gcggtaaact cgttgctgtt gactgtgact acggtgtagg
    25741 aggtctccaa ctcggtgaag gtacagcggc ttctggtaag tctccactta tcgctaaact
    25801 cgctgactaa tcgaggttca tctgtctact ttacacagta gaacattacc aaattttatc
    25861 acggcgcagt ccccactggg gctgcgtcag ttgatgctta agacgaatgc aagagcgggc
    25921 gcgtcttttc aatatttcga cattaaccaa ttcacggatc ataacggtaa acttcgttgg
    25981 atcgcgtggt actatagtga cataggaagc attggtgaat tagatggcac agccgaacag
    26041 cttcagggat agagactaca caaagtttgt agattctcca acgaggccag gaaagtcagc
    26101 agtagaggta gtaggtgatt tgaccgtttc tggtggtgaa tttgcgcccc catctaactc
    26161 tacttgctac acatacgaga ctggaacaga cggaatttat tttacagaga tttataaatt
    26221 ctatgaatca ggaacaccag caactccagt aaatcttctt aagacaatca ctctttatta
    26281 tacagacgca caattccaga atgattttgg aggtgtggtg tcatgagaaa aaagtttaat
    26341 ccattttctg gaacttttga tttcattgga acaacatcaa gtgattcaga tagtaattgt
    26401 gtaattcttt taaactctgt tagcattgaa tcaactggtg atttagaaat tactgatgag
    26461 gcctgtatag aagtgaaaaa ttctggagtt acattcgcac aaagaatcaa taatactgtt
    26521 accgtaaata ataacggaga aatgattatt gatgcaaact ctgaagtaga ggtatcagcc
    26581 taatgtcaca acttaaacta caaatcggtt cagtacctac aacgccagcg agtggttgga
    26641 ttacaattta tccaaaaggc gataaaaagc tatattacaa agacgatgct ggaaacgaat
    26701 cagttcttga tatttcagat cgcctcattt tgaatggccc tggtgctcct gcatcaacaa
    26761 ctggtcaaga tggtgattac tacattgata atacaaacac gcttctttat ggaccgaaga
    26821 cttctggttc ttggggtagt cctgttacta tggttggtcc acaaggtcct acgggagcaa
    26881 ctggtgcaac aggagcaact ggagcaacag gagcaactgg agcagcaggt acagatggaa
    26941 acgtatggcg taacggaaat ggtaccccat caaatggtct tggtgtagac gatgattatt
    27001 atcttgatga tgtaacttct gattactaca agaaggtatc tggagcatac gtgcttcaag
    27061 gaaatttaaa ctcaagaaat acaaatattg atggtggaca cgctaacact atctatggtg
    27121 gaactctgcc aattgatggt ggatattcaa cttcatttta aggagataca atggcacagc
    27181 aaattcaact aagaagagat acatcatcaa actgggcatc aaacaatcca atacttgctc
    27241 aaggtgaact aggtttagaa acaaacaccg gacaatttaa aattggtgat ggttctactg
    27301 cctggaactc tctctcttat ggtggtttag ttggtcctgc acagacaaat cctattatgg
    27361 gttatggttc tggattagat ggagatgtta ctcttagtgc tggtatcacc actctcacaa
    27421 gaaatatgta ctataacaat cttacgttga ctggtactgc gagaattaac gtatcaggat
    27481 ttaaaatctt cgttaaagat actcttgata ttactgttgc tggacctgct gcaatttatg
    27541 ctattggtaa cgatggagca agcgcaacaa ctcaaactgg tggagctgct ggtgctgcat
    27601 tggcatcaac agcaaacgga ggatcaactg ctggatctgc tggaggtact ggtactgctg
    27661 ccgctggagc tgcaagtgct gccgccacag tagcatctcc tgcaaatgga ggcgcatctg
    27721 gagcaagtgg tgccggtgga tctggttcaa gtggtgctgg tggtgcatct ggtgctacag
    27781 ttatcccaac tctatctttg ccagttaacc attttcaaac agaccttaac agaggtaact
    27841 ctttattgct tggaggaaac ggagctcgcg gcggcggtgc tggaggtgga gatggaacta
    27901 accttggtcg cggcggtgga ggcggcggcg gcggtggagg agttcttcat gtattttcca
    27961 gaaaaattaa ccgtagcgga tcaacatcag ttggatgttt ttctgttaag ggtggaatgg
    28021 gtggaaacgg tgctactggt tctgttggta acattggcgg cggcggtgga ggcggcggcg
    28081 gcggcggtgg ttgggcattc attttgtacg atcaactatt aggaacaaca tcaacacaaa
    28141 caattgatgc aagtggtgga gatggtggta ttggtgggaa tggatttggt actggtatcg
    28201 gaggaaacgg tggaaacggt ggtgttgctg gaagaattac catagtaaat gcaatcaaca
    28261 gaacaggttt tgatatttca gtaggttctg gtgctggtac tggtggaact gcatctggtg
    28321 taactggcgg tgctggtggt gctggagatg ttttaaagct taatcttcaa ggggtttaat
    28381 aatgattact agagacattt tagattataa tggcgatgtt atcggaagca tggattttcc
    28441 agatggaaca tctgatgaat atatttcatc tgtattatct caatatgcag ttgaaccaat
    28501 agtggataac gattcttatt taaaattctc cattattgag cgcaagaaat ttgcagatga
    28561 gttgctagag cgtttcaaac taagaaatat aaacgaaggt attaacgcac ttcagggaat
    28621 gtggatgcat cacagactta gagcgtatcc agtatctttc ggtggaatga attttactat
    28681 tgatattatg aatcttgcaa tcagtggtga tcttgagatt gcttgcctgt gtcttctata
    28741 tggagatact gatgatggca cagaaacata tcattggcta acttcagata gaaaagcgta
    28801 tctaattaat gagcttaaat cattcttagg gtggaactaa tgaagaagat gacaatgatt
    28861 attcttggaa ttgtttttgt atgtctcgtt ttgtgggatg tttacgcaat ggttaagagc
    28921 ggtggaagtg aagcgtctat ttcatctgta atcatcaatt ttgcgtataa aatgcctatg
    28981 ttttcattct caagcggatt tgtatgcggt cacctatttt ggagaatgcg aacaaataaa
    29041 gacacaaaac atattgatga ccagggggaa tgatggctat ttttggaaat attgagctag
    29101 aggatattgt tcaggttaat gacaagacaa gacttaattg cgtaaaatct tttgtatcta
    29161 aagatgaggc tgcaatttct ttggttagaa ttaagcctga agcatctggt tcatttatcc
    29221 aagtaggaac tacaaaccaa aaagaatact acctagactg ggaatattct acccctggaa
    29281 ataaaacagt aacgctagaa attacaacaa atgaaacagc gcaaacgttc actaaaaata
    29341 ttatggttgt aaacgctgct agtgataagt tgctgagttg cgatgcaaac cttattgcaa
    29401 tagagcctga tattttaaaa tgggttccta ttggacgtaa ctcattctta aacgtacaca
    29461 gaaaagcact agacttaatt cttgattggt tagattcgat tcgagtgtgg cgagatgatg
    29521 gaacaaagct aacaaaagaa gacatttcct acaccgatga tctaaataag ctttcaacct
    29581 acatcacact tgaactgatc ttcatgggaa tttccaacaa ggtcgatgat gtgtttttaa
    29641 ataaagctcg tgagtacaga aataaagcac aagagatgaa aaaccgtgga cgcattcaag
    29701 cggatttaaa cggcgatcaa gagcttgata aggctgagaa cgtagatatg cgatcttttg
    29761 atttggtgag acgatgagct tacaatcggt tagaccatac atcactgcta gattaactgc
    29821 acttggatat aaagagcacg ttgacccatt caatgatgag aatattccag cgagtttagt
    29881 cgacggagca ttccatcagc taatgcttcc tattagtgga gttgatcgaa acagcgtagc
    29941 gcaaggacta gacgttcctg ttcaggttaa agtcttgatg actggatata gaaacccaga
    30001 agaggcgcta acgcaaagca ttgccaatgc tgaatcgatt atttcagata taactttaca
    30061 ctctaactat gtgaataatg ccccaccaat cactggcgtt tttcttgatt ctttagagtt
    30121 tggcccgtac tctgtagaga gtaacgataa catcgtacag gctgtatttg tgtttcgttt
    30181 caaggtttat acttgcgtag aataaaaccc aaaggagggg tgaaaaatgg cgtgtaatac
    30241 tcaaacctac aatctaggta ttcggaatat tcttttgggt tctgatcgac cacagaagac
    30301 ttgcatcttc actaaagcag acgtttctag ttcattgcaa aataaataca ttgttatgca
    30361 tgagccagta acccaggcta aacattattt ttggtttaac gtagcaactg caggtactga
    30421 tcctgctgtt ccaaatgcta ctggtcacgc tgttgcgatc tcttcaaatg ctactaaaaa
    30481 tgctgttgca actgcccttc aaactgtaat cgatgcactc gcatgggctt ctgcaactgt
    30541 tagcggtaac gaagtcgaag tcactatgac agcagacgga tacgcatacg aagcgcgcga
    30601 tgctctcagt tctttgtcta aaactggctt ctccgtaaac gtagcacaat ttggatcagt
    30661 tcaatttgat gcaggtggaa ctaatggaga catcactttc acaatcgaag aacagactaa
    30721 agaaattaag tctccacaga ctggcgattt cttgcttgct gagattcgtc gcggttctaa
    30781 cgtaactgca tctttcgagt tgaaagactc ttctgctgaa tctatccgcc gcgctcttaa
    30841 cttctacggc caaacaatcg ttactgacga tgcggcttct gaagttatca ctggtcacgg
    30901 atcaaataac ttgttcaagt ctacagatga tgttgcagat caagttatct tgcgcccaac
    30961 cgataaagct ggtgatgcag acgcttctga agatttcact attcacaagg ctcgccttaa
    31021 acttggtgaa attacattca gtgcagaaaa cgaacttgta ttgccgatcg aggttatggg
    31081 ataccttgat acaagtaaga gcggatttgc taacttgttt agctatggaa atggctctgc
    31141 acttcctagc gcataattga gagggataaa tggaatttgt ttttgaagct aaagaaaaga
    31201 aaatgacctt gaacttgtac gggactaaat accaaattag agtccctaaa atgaaggagt
    31261 cacaagaact tcaaaacaaa attaaaaatg ccgatcctgg tgatgtagtt cagatctata
    31321 tggacttctt cgctgggatc ggtcttccgg ttgagtcatt ggattcattg gatgctatcg
    31381 acttccaaga gttgatttca ttcatcctgt atccaaaaaa aagttaactg attacgccat
    31441 agaaaaagca atattgtgcc gattctatgg ctggtcagat gactatgttg agagtttaga
    31501 tataagaaca ttcattgatt actggaacgc tgtaacggta atcgaagcta gagaacggtt
    31561 agttgattta aacgtggcaa tgattccaca tgctgaagat aaagatcgca agaagttcta
    31621 taaggaactt gaaaagaaag cttatccagt gagaaaatca acaggtaaga agctcactaa
    31681 caaagagctt atggacatat tgagcagacg ataaaggaat attgtggctg atcaaaagat
    31741 tgaattagaa attgtcctag acgatggctc gattaagaag gcctttggca ctattcgcaa
    31801 ggaagctgaa gagacatcca aagaaactca agggatgttc ggagcttttg gaaatgccgg
    31861 agtcgcattc gtagcattaa accaaggttt agaactattc gccaaactcg gtgattcaat
    31921 aaagggagtt gctaaagaag ttattaacct ctctttagaa gctgaaaaag ttcgtgcaac
    31981 acaagctcaa tttacacaat tcactcaaca ggcaggaatt gcgacagaac aatttagcaa
    32041 agaaattgaa aagtctattg ctggactaat agacgatgac gacgccctgc aaattgcaaa
    32101 tgaggcaatg attagacttg gatcaacggc ccaaagactt cctcaaattt ttgaacttgc
    32161 tagaaaagct gctgctgctg gatttggtga catggcatcg aatgctgagg cattcactac
    32221 agcaattcaa acaggacaga caaaggctct gaagcaacat ttggggttaa ttgtagatgt
    32281 aactaaagca caaaaagaat acgcgaaaga ggctggcatt ggtgttgata agctaaatga
    32341 acaacaaaga gcagttgcta acgctaatgc aattctagat gccgctagta agaaattcaa
    32401 taacgtagat acagacgtta agtcgttctc ggaatcaatt caaagattga atgttgctat
    32461 tgctgaacag ggtgaaaaaa ctgctgtttg gtttgatcgt gtatttggat ctgcaacaaa
    32521 aggaattatc aatacattaa ctgatgcaat taacggaaat ggagccgctg ttgctgctca
    32581 aaaagttaaa gttgaggacc ttggtaaagc aattgaagat gctgaaaaga aaatcatttc
    32641 aattcaaaac aatctatcaa atgctagaac agatacccaa ttcagtttct taaataatca
    32701 gttaaatgaa actaataagc tcttagatgc tttaagacaa agacagtcag attctcaaaa
    32761 tttcgctgct cttggaaatg ccaacgatct tctaagtaaa aatattaagc tttcatggga
    32821 gaaagaacag gccgatgcgt ctaattctaa actagcgcaa gctagagcag aagaacaaat
    32881 gcgtattcag caagcattgc ttcaatcttc tcaatcattc caacagcaag aaatcgcagc
    32941 aagacaacaa agcgcacagt taattataga cgatgataaa cgccaagaag agatggctgc
    33001 aattcatcaa gagcagatta accagattgc tattaatggt cttattgcta gacagaatat
    33061 tgaaactcag ttttcaaatg aaaaaggatt taacgacgcg caacgagcag aattaagaag
    33121 acagcagatt gaggcagaaa atgcacagat tattgcttca caagaagcat ttcaaaatca
    33181 atctcttaat agctttcaaa agtattctat tgagcagcaa aagaagtttc agcagcttgg
    33241 ttctatggtg aagatcactt tcattaatgg catcggtagc gcatttgttg ctttcggtca
    33301 ggcacttgct aaaggcgaag acgctatggc agcattcggc aaggcattgc ttggcgttct
    33361 aggtgacatt gcaattcaaa tggggcaaat gtttatctgg caagggttag gatttagtgc
    33421 gaacccattg atgcctggga gtggtgctgg attaattgct gctggtattg gtttatcaat
    33481 tcttggtggc gtattgaaag gattggctgg tggaggtgga gcaacaggta gcgcaagtgc
    33541 tggaggagga gtggcagcag gtgggccaga aagcgggtcg tttaatgcgg cagctcctgt
    33601 ggccgatact cgtattgcac ctaacactgt cgttaatttc accgtccaag gcgatattct
    33661 tgattcagac tctactcaga cacgaattgt tcaattacta aatgatgcca ttgataccaa
    33721 gggcgcagta gtgaggggct tataatggcg ctaatgacta gagcgaaatt ctatttcggc
    33781 atcgaggttg agcaagataa taacttgatc gacattgacg aaggtagcgg tgaggtttct
    33841 attgagattc ctgtaggctc atactctcca aagcaattta gcgaaaagct ttctgctgtc
    33901 ttaactagca gtttaactca aactt
//
LOCUS       DAOQLJ010000006        31901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_441_length_31901_cov_6.424637, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000006 DAOQLJ010000000
VERSION     DAOQLJ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 31901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31901
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_441_length_31901_cov_6.424637"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /gene="rseP"
                     /locus_tag="PK522_00990"
     CDS             complement(<1..716)
                     /gene="rseP"
                     /locus_tag="PK522_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNC40338.1"
                     /translation="MTILTTIIAFIVALGVLITFHELGHYWVARWSGVKVLRFSIGFG
                     QPLFKKSIGKDQTEWVLAALPLGGYVKMLDEREGEVLPHELPRAFNQQSVYKRFAIVA
                     AGPIANFLLAILLYWILFMLGVNGVKPVIGPIKQATPAAFAAFEEGEIIQKIEGESVS
                     TWQDARWALLTQAVDRNPVVVVETVNSHGQTALRKLDFSQIHTDDLDANFLEGIGFIA
                     HQPSMDPIIGQVIAGGVGHQA"
     gene            complement(782..1969)
                     /gene="ispC"
                     /locus_tag="PK522_00995"
     CDS             complement(782..1969)
                     /gene="ispC"
                     /locus_tag="PK522_00995"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNC40339.1"
                     /translation="MTTVRKITLLGSTGSIGENTLDVVARHADRFQVCALTANKSVEK
                     IFLQCQRFHPQYAVMLDHKSAAALENKLKAVGLDTQVLSGVESLEKVASLPEVDIVMA
                     AIVGAAGIRPTIAAARSGKSVMLANKETLVMAGRIFIDIVKQNQATLLPIDSEHNAIY
                     QSLPKYFDGNLTTAGVRRILLTASGGPFRRFSERELEHVTPEQACAHPNWVMGKKISV
                     DSATMMNKGLEVIEAHWLFDAAPEMIEVVIHPQSVIHSMVEYIDGSVLAQLGNPDMRT
                     PIAHALGFPDRMESGVKSLNIFEIARLDFEAPDLGRFPCLRLAYQALAKGGNMPAVLN
                     AANEVAVESFLTGKISFTAIPDMIEHVMGSVSSAEIDTLDDVLAADSAARLAAQEWLL
                     CKV"
     gene            complement(1966..2793)
                     /locus_tag="PK522_01000"
     CDS             complement(1966..2793)
                     /locus_tag="PK522_01000"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNC40340.1"
                     /translation="MLKLRIITAICLLLFFLSALFFLPPILWGMLLLGMAIVASREWC
                     TLGQFNTKQTLVYLIITTILGGELLFVLSEVVDVNPITTDIVWPYAVSLVFWVLIVPF
                     LLTLSKPARNTAMMMLIGWLVLLPTLLAMYQLRTIDPMILLIFMSVVWISDTAAYFVG
                     KKFGKHKLAPQISPGKTWEGVMGALLAVLCYAMALGYYLEETISFVIMLTFAVIGLAI
                     LGIIGDLFESQLKRQVGIKDSGTILPGHGGVLDRIDALTSTLPTATLIYLIYSHVTL"
     gene            complement(2845..3645)
                     /gene="uppS"
                     /locus_tag="PK522_01005"
     CDS             complement(2845..3645)
                     /gene="uppS"
                     /locus_tag="PK522_01005"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNC40341.1"
                     /translation="MPRNPSSTREIPVTNDTPRHIAIIMDGNGRWAKKRFLPRVAGHK
                     HGVEAVRAVIKACIERKVGYLTLFAFSSENWRRPEEEVKFLMQLFLGALEQEVTKLNE
                     NNICFKVIGDLSRFDPKIIEFIRQGEKLTANNTRLIFTVAANYGGRWDIMQAIRKMMI
                     QLRDLNENFEEEDLSQYFSMSYAPEPDLFIRTGGECRISNFLLWQLAYTELYFTETLW
                     PDFNAHELDLAIQSYQQRERRFGRTSEQLQDRTSVDKIRPKIAIKNKV"
     gene            complement(3650..4207)
                     /gene="frr"
                     /locus_tag="PK522_01010"
     CDS             complement(3650..4207)
                     /gene="frr"
                     /locus_tag="PK522_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNC40342.1"
                     /translation="MIADVKKAAEQKMQKSLEALKTDFGKVRSGRPHTGLLDQVLVDY
                     YGTPTPISQLANITLPDARTIGVTPWEKKMLSPIEKAIRDSDLGLNPMTTGDMVRVPM
                     PPLTEERRRDLTKIVKNEAEAARVAMRNTRRDANAQLKELLKDKKISEDDDRKGQDDI
                     QKLTDRYIVEVEKLLQTKEAELMAV"
     gene            complement(4227..4946)
                     /gene="pyrH"
                     /locus_tag="PK522_01015"
     CDS             complement(4227..4946)
                     /gene="pyrH"
                     /locus_tag="PK522_01015"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNC40343.1"
                     /translation="MATITYKRILLKLSGEALMGDDKYGINRDVVEHIVIEITGAIQL
                     GVEIAIVVGGGNIFRGVKSAGAGMDRVTADYMGMLATLMNALALQDALSRSGIISRVQ
                     SALRIDQVVEPYIRNKAIRYLEEGKVVIFAAGTGNPFFTTDTAAALRGMEINANIVLK
                     ATKVDGIYSSDPKLNSDATRFKSLSFDEAITKNLQVMDATALTLCRDQNLPIGVFSIF
                     KVGALKRILLGEDEGTSVNNR"
     gene            complement(4961..5857)
                     /gene="tsf"
                     /locus_tag="PK522_01020"
     CDS             complement(4961..5857)
                     /gene="tsf"
                     /locus_tag="PK522_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNC40344.1"
                     /translation="MADITAGMVKDLRELTGLGMMECKKALVETNGDMKAAEDLLRIK
                     SGAKASKAAGRIAAEGVIGAYVSADQKQGGLVELNCETDFVAKNTDFINFAKALAQLV
                     ADKKVNNADELSSMVLPEGETVEAFRQSLVMKLGENISIRRSACYTAKGKLATYLHGT
                     KIGVMIDYSCEDEVLGKDLAMHIAASKPLCVSSDQVPHDLVERERQIFKAQAAESGKP
                     ANIIEKMVEGRVSKYFAEVTLLGQPFVKDPDQTVEKLLAAKSGKVHAFTLFVVGEGIE
                     KKSGDFAAEVMAQVSQMKENKS"
     gene            complement(5917..6642)
                     /gene="rpsB"
                     /locus_tag="PK522_01025"
     CDS             complement(5917..6642)
                     /gene="rpsB"
                     /locus_tag="PK522_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNC40345.1"
                     /translation="MSVTMRQMLEAGVHFGHQTRFWNPKMSQYIYGERNKIHIINLEK
                     TLVMFNEAMQYLRNVTAKSGTVLFVGTKRQARDILSEEAIRCGSPYINQRWLGGTLTN
                     FKTIRQSIKRLQDMEASVQNGILDKLTKKEGLDFQRQLEKLNNSLGGIKDMRGLPDVM
                     FVIDVGYQKGAIVEANKLGIPVVGIVDTNHNPAGVQYLIPGNDDSSQAIRLYARAAAD
                     AILEGREQSIQEIVEMSGEAAES"
     gene            complement(6861..7589)
                     /gene="fnr"
                     /locus_tag="PK522_01030"
     CDS             complement(6861..7589)
                     /gene="fnr"
                     /locus_tag="PK522_01030"
                     /inference="COORDINATES: protein motif:HMM:NF008365.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HNC40346.1"
                     /translation="MSALKKYIASQAISKCASCSLRELCLPVGLDAHETEVLSDLIDH
                     KYKIQRGEYLLHAGMRFKSLYAVRNGFFKSYILDSEGRQQVTGFYMTGELLGLDAISA
                     EIHTCDTVALEDSEVCEVPFERLEEISCSIPLLMHQFHKIMSREIVQDHRVMVLLGNM
                     KAEERIAAFLLNLSERFVKRGYSATSFVLRMTREEIGSYLGLKLETVSRSFSKLQDEG
                     MITINNKHVELHDIHRLTRKISSY"
     gene            complement(7649..7954)
                     /locus_tag="PK522_01035"
     CDS             complement(7649..7954)
                     /locus_tag="PK522_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC40347.1"
                     /translation="MKKKLWLLSMVGVLALVSGCSDKYTPSASATPESIFKEACVKCH
                     SPVNGKVMALSPEMANTAAIIERIKNGKGFGMPAFPNLTGETAEGLAQYILDNSVTK"
     gene            8387..9976
                     /locus_tag="PK522_01040"
     CDS             8387..9976
                     /locus_tag="PK522_01040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC40348.1"
                     /translation="MKNSKTLTEIEIAREALRQLAVNRKLPTPDNYRSLYYQIAGVEI
                     IDFFPEKTLKIIIEKLPRNNSDQQQIIQSFEAAVSKGDWESLGAILIDLLSGNVIKQN
                     PISNDHSNDHHLEKDNVQPNETGKLITSILPRELQDQLGRLFGDCLSILLSTSPEISK
                     DAESLAREVRVISESGQLSQFTDKLKQFNYQLQYAAEDQGELRTSLLKLFQLVIDNIS
                     ELVVDDAWLQGQIAIVREMITEPLDLRLIDNVERQIKELIYKQGLLKKNLIDNQSRLK
                     LMLSSFVDRLAEFTQTTGDYHASLGVYAEEISQAEDISELSDVLDRIIQQTRQVQSSA
                     KRSHDELLELRSRAESAEKEVDRLQSELLHASELIRHDALTGALNRKGLEEAIEREVS
                     RKYRDGSSLCIALLDIDNFKKLNDTHGHEAGDDALVHLAAVVRKSLRPQDTLARFGGE
                     EFVVLLTGTGVNDSVTVMQRVQRDLTRCFFLHQNEKILITFSCGIAELAENEDPKTTI
                     KRADQAMYLAKRSGKNRVLAA"
     gene            complement(9995..11647)
                     /locus_tag="PK522_01045"
     CDS             complement(9995..11647)
                     /locus_tag="PK522_01045"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC40349.1"
                     /translation="MKSIKTKFVTFAIIAISIPSLSLGLLSYFKNEGLVRNHVTRELR
                     VLASNVSRELDLWINENYHAVSVLSVSNLIINELALSARDSVSKSSNHQGSRQLVLSK
                     YLSSFQEKLPTIVELTLFDLNGNVVASSIPESLNSVIRGNEQHDMLTSNIKIKPPYRS
                     DRYDTAVLELYSPVLSYDEKVIGSILATLNMEKFMSSLVDNTDFSLGEITLLGYAGNT
                     ILSSNHKINYSASLDSKTLKQLQDYGGEALAFKGLTHSKVIGLISKSKEAPIIVLVER
                     DYAEAIAAWIELRNLFMSLVGLLIVIVGGIAFYIGHSIVASLSYLIEGTKRILNGDLN
                     VQINEIKADEIGQLTASFNKMTENLRCSQAEILAAHEAMQKQNQQLKVLSITDSLTEL
                     YNRNKLDAILIDQIARFERNHRPFSVVMMDIDHFKTLNDNYGHVIGDEILVSVSQAIS
                     KSIRSVDFAARYGGDEFVVILTETDAASAVITAERIRAQVAINVNSQIKEVSLSITLS
                     MGIIQSEIDDKTPKNILSRVDGALYKAKNSGRNRIYCAHHPS"
     gene            complement(11819..12547)
                     /locus_tag="PK522_01050"
     CDS             complement(11819..12547)
                     /locus_tag="PK522_01050"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HNC40350.1"
                     /translation="MNINQDYLIDSQNEVTHILRTFLEQQTKVLLYLIGENISPVPTT
                     VLNINPSQDELIVGYYPEAYFNQRAQLAERLVCVATHNGIRVEFESFSIGKTWFGRDA
                     AYRLDFPKVISRVQRREYFRMSPLMKQQPRCAILDKDQQVIGFTILDISCGGMSIKHD
                     RPNIKKLEVGEIYPDCSIELASPIGLIKTPIRIRNISEMPSKSNQIYTRYGCEFIDLP
                     SKTRMMIQRYIIKLEQDSRSKDWM"
     gene            complement(12576..12902)
                     /gene="fliE"
                     /locus_tag="PK522_01055"
     CDS             complement(12576..12902)
                     /gene="fliE"
                     /locus_tag="PK522_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNC40351.1"
                     /translation="MDKSNINQILTQLRLTSDAISGSLKPDNAQFNPKEADFSNILKS
                     AVDQVNATQQTAGQLSQEFMKGGSDTNLHDVMISLQKANISFQSMIQIRNRLVTAYQE
                     IMNMQV"
     gene            complement(12923..14248)
                     /locus_tag="PK522_01060"
     CDS             complement(12923..14248)
                     /locus_tag="PK522_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNC40352.1"
                     /translation="MNELSILIVEDDQDLLEAICTTVKLSGYKALGASNGTAALDLLN
                     SQQIGLVISDVQMQPIDGNILLKRIKETYPDIPVLLMTAYGEINKAIAAMRAGASDYL
                     LKPFSPDYLLTYIKNYLVPANNQRNNYEEVIAKDPRTSAMFSLAKRVAESSATVMLIG
                     ESGSGKEVLARYIHQHSPRLGKPFVAINCAAIPENLLEATLFGYEKGAFTGAAQAQPG
                     KFEQANEGTLLLDEISEMPLGLQAKLLRVLQEREVERIGGRKPVKLDIRLLTASNRDI
                     FSLVKQGAFREDLYYRLNVFPLEIAPLRERPLDIEPLVHRILQSPNKGIKGRNYSLTK
                     LALLALRQYSWPGNVRELENVIQRAMILATDEIDVMHLNLPEQARSDVTLPFDTSDLS
                     QSKDIKSLEREHILRTLAEVNGSRKLAVQRLGISERTLRYKLQQYRLVG"
     gene            complement(14261..15484)
                     /locus_tag="PK522_01065"
     CDS             complement(14261..15484)
                     /locus_tag="PK522_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC40353.1"
                     /translation="MKAESNVRLGKSDADHHQLMHAFTVFSQASEQLSTVYRDLQQQV
                     EHLTNELALANGELQHQLAQKENLSQQLSLLLSALPGGVVAIDNEGVIESANPVALSI
                     LGEPLLQCTWQQILEDRLLATDVANEWNLRKETHSDFRRISIESSASEDSGKMVLLIQ
                     DITEAYTNQERIKREQRLAVMGRMAANLAHQLRTPLSTALIYATHLSNSELPTQERSS
                     FAVKTIERLQYLNQLINSMLRFVKGELVKKEVIDISVLLSDIQSLMLPHMLKKNLQFV
                     VKDGGEGAALTVNYEELCSSLIALLENAMQASPVGGRISLTCDLTTDEVFFTVADQGN
                     GIDIANEERLFEPFFTTRPEGTGLGLAIVHSAIKQMGGDVQVKLLTDRGSEFILRLPR
                     TSLSNITNSNLSSSI"
     gene            15716..17359
                     /gene="fliF"
                     /locus_tag="PK522_01070"
     CDS             15716..17359
                     /gene="fliF"
                     /locus_tag="PK522_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNC40354.1"
                     /translation="MATVSEEIGTKTNRFSAIAQLSGQKKLGLMLAAAASIALLVGIA
                     IWSQSPDYRVLYTNITEQDGGAIINALQQMNIPYQFSEGNGTILIPGTQVHEVRLRLA
                     SQGLPKGGISGFEILENQKFGSSQFLEQVNYQRALEGELARSIQSLSAVQSARVHLAI
                     AKPTVFVRERQQPSVSVLLNLYPGRVLTTEQVSAIIHLVSSSIPSLPVKNVTVVDQNG
                     NLLSTPKKEGNETGLDDSQLKYIHELEQNINKRIETILLPITGPNNVRAQVTADVDFS
                     RTERAEEIYKPNNIATEAASIRSQQKSDSMSANRPESGVPGALSNRPPAAAEAPVDVA
                     AGEGDTKNQVPVDTRSESTTNYELDKTIQHTRQSVGRINRLSAAVVINYRGKMDEAGN
                     LIHEPLAAEEIEKINSLVKQAMGFDENRGDTLTVTNSQFNILEEEIAEIPFWKQPDMI
                     LLAKEIGKQLIIAALVLFFLLKILKPFLKTLLPPPPPPPPAPLQATAGLEESNNLALS
                     HQQEVVSHEQNITRAKQLALNEPIIVANVIKEWVSDNER"
     gene            17349..18347
                     /gene="fliG"
                     /locus_tag="PK522_01075"
     CDS             17349..18347
                     /gene="fliG"
                     /locus_tag="PK522_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNC40355.1"
                     /translation="MSDDGILKSAILLMSLGEEEAAEVFKYLGPKEVQKLSEAMSSLN
                     NVKRDKIESVVHEFCTLASDRTSLGQNSLAYIRNVLIRALGEEKAASLLDRMTEKGND
                     TSGIENLKWMDTAAVAELIKNEHPQIIATILVHLERDQASAILALFTERLRNDVILRI
                     TTLDSIQPTALRELNSVLNKLLSGSDNIKKAPKGGIRTTAEMLNFIPSSLEGNIIEHI
                     TQYDESIAQKIMDEMFVFDDLLTVDDQGIQLLLREVQSDSLIIALKGAKEELRNKIFK
                     NMSQRAAEMLREDLEIKGPVRVSEVEAAQKEMLKTLRQLADDGKVALGGKGGDGFV"
     gene            18443..19108
                     /locus_tag="PK522_01080"
     CDS             18443..19108
                     /locus_tag="PK522_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HNC40356.1"
                     /translation="MNSPSVIPKKQLSSFQKWELGSFETNLANNTTQLDESVDQVAKE
                     TISLPTAEQIEAIYQQAKNEGYQAGYQEGKQHATVEMETTIQHLQVLMVKFEEELQHI
                     DQTVAQELLELAIEFARKIVTQSLELKPELIIPIIQDAIQHLPSAMQPLHIILHPEDA
                     TLVRIQLENQLSHSKWKIYENSQIERGGCKVEAGGCEIDATLASRWKTILSKVLLENH
                     QPN"
     gene            19116..20513
                     /gene="fliI"
                     /locus_tag="PK522_01085"
     CDS             19116..20513
                     /gene="fliI"
                     /locus_tag="PK522_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HNC40357.1"
                     /translation="MNHCSSWQSFLNNCKQIVSHSNSLIISGTLTRVAGLVMEAVGLR
                     LAVGSTCTVFLPNGHNVSAEVVGFSGDRLFLMPSSDVYGLVPGAKVVPTEYASDHPRI
                     GKNWRPRRRAADRTKQINVGYELLGRVLSGAGEPLDQLGIIHTEQTVPLYSRPFNPLE
                     RAPIDMPLDVGIRAINTLLSVGRGQRMGLFAGSGVGKSVLLGMMARYTKADVIVVALI
                     GERGREVKEFIENILGQEGLARSVVIAAPADTSPLMRLQGAAYATAIAEFFRDEGKNV
                     LLIMDSLTRFAMAQREIALAVGEPPATKGYPPSVFARLPQLVERAGNGRQGSGSITAF
                     YTVLSENDDPNDPIADNARAILDGHIVLSRRLADSGHYPAIDIESSISRVMPHLASQE
                     QLEWTRRFKNLYARYHRSHDLISVGAYVPGSDPELDLAIKIYPALESFLQQGIFEHEN
                     IAESLQKLSKLLTLR"
     gene            20554..21000
                     /gene="fliJ"
                     /locus_tag="PK522_01090"
     CDS             20554..21000
                     /gene="fliJ"
                     /locus_tag="PK522_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HNC40358.1"
                     /translation="MATTQSLKTLVEFAQQKMDASAKKLGFLNSKQQEAEKKLNLLID
                     YRRNYQAHFVNTKNTGMNSIEWTNYLAFIGKLDAAIVEQRKVTINAQKETEIVKTEYQ
                     DSRRKLKSYHTLLQRIKHHHAIKLEKHEQKLLDEFVTIARQHKTEG"
     gene            21075..22280
                     /locus_tag="PK522_01095"
     CDS             21075..22280
                     /locus_tag="PK522_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNC40359.1"
                     /translation="MLNLTSIQETLLPSVNPDINSPLTDSAGLNQSNSTPFQEILGKT
                     LAEDLKTELSADITDTETAISNELLIEATNAVEIPISLMAETNTANSNSMTSFSLADS
                     SNILRGAQNQINPSLLNTIEENILQITPLLDTALENSDAPLATLISDNLKTTISTSSA
                     SNQTYTTFLQAQAYQNNSIPSINTSPYSALNQFTQLLTRDASSEEVDITPVNFAEIGK
                     ILPISPDSSLVAQSNLITQTVSPSSSTPLLPIHPHHVATEFGKPAWGNDFNQQITWLA
                     TQKHQIAELHLHPAELGPVEVILHISNDQSTQVSAQFTSPHLAVREAIESALPRLREM
                     MAENGITLGNTTVGAETSQQHANQQHGSIASNANENSTTTVATIQGLSTEFNGSVLPR
                     HQGIINTFA"
     gene            complement(22358..23263)
                     /locus_tag="PK522_01100"
     CDS             complement(22358..23263)
                     /locus_tag="PK522_01100"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC40360.1"
                     /translation="MKKTLLIIGCGDIALRTAPLLARYYRLLGLTRRTENFSKLRSHQ
                     ITPLLGDLDQQASLKRLSGIADAIIHLAPPPNMGLKDTRTRHLLAALSRQHSRAKGRI
                     PHRLVYISTSGVYGDRDGQWVGEHNPVKPRNDRAWRRFDAECQIRKWGTDTGSNVTIL
                     RVPGIYAIDRLPIARLKQGTPALLEKEDSYTNHIHADDLARIIVAAFRYGKNSRIYNA
                     CDDSSLKMGDYFDLVADTFKLPHPPRISRTQAKEQIPAGLLSFMEESRRLTNDRMKRE
                     LRIKLLYPTVHEGISKVIPDKNCLR"
     gene            23302..24348
                     /locus_tag="PK522_01105"
     CDS             23302..24348
                     /locus_tag="PK522_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HNC40361.1"
                     /translation="MSYQVIVKPSNHTFTIETGESILQAALRQGISLPYGCRNGSCGA
                     CKGKILQGHVDYGRYDETTLTEQDKQNGLALFCCASALSDNLVIECHEVDTLKDIRIR
                     TMPCRVHKLDRVAPDVMVIYLKLPTNERLQFLPGQYIDILLKDGKRRSFSLANAPHDD
                     EFLQLHARNYPGGVFSEHVFTRMKEKDILRIEGPLGSFYLREPPSDKTPLIFLASGTG
                     FAPIKSILEHILSTLPFQESHRKLLLYWGVRVKADLYLNDLVEQWANTYKNFSYIPVL
                     SDALPTDQWKGRTGLVHQAVLEDIPNLASYQVYACGSPLMVKAAYQDFTQLRNLPAAQ
                     FFSDAFIPSDNQMP"
     gene            complement(24352..25530)
                     /locus_tag="PK522_01110"
     CDS             complement(24352..25530)
                     /locus_tag="PK522_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HNC40362.1"
                     /translation="MRLVLWVLFLFAAAVGIVYVAQFNSGSVLFNVPPFKVELTINNF
                     FILLIVAFFIFYVLLKILARILGFSFYFRRKRINDRTLVGLKAFFEGKYDRAQKAAAS
                     ALRLNSSPIINGINAALAARAAHKLEDYTARDEFLAASEEVAPGEHTLRLITQAELLL
                     EEGRHKEALDALHSLYSLGGLQSITALQLELKAQQMAQNWDAVMDLVNILANRYPYGK
                     GSIEQLKHHAQQESIKKNSTNLDLLNKYWHSLNSMEQLDSRLAATAAKGYIALNDMAT
                     AQRVIEQSLDSKLDNELITLYSKCLGNSVSWQIQRAENWLSKNPNNAELLLTLGKLCT
                     YCELWGKAQNYLEASLSIEPSRAAHLALAQLFEKLDKRELASDHYHKGLGFTLQKQAI
                     "
     gene            complement(25597..26304)
                     /locus_tag="PK522_01115"
     CDS             complement(25597..26304)
                     /locus_tag="PK522_01115"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNC40363.1"
                     /translation="MERIQHTKHRSSQQGALLAFLVLIIVLAAMGWFWAKKSGHLVDL
                     KHAIAGYLDKVDLTGKKPRQLLADIETELSNAEKQLGAIKATLSENDQAIAEKLASIS
                     QVIETLPLAMDRHFVKLDFALPHSETLQNSKLYLFLSEICKDLQKLISIQKIDDSSIE
                     IISPAQADQLRKNIQLKLTLAQLALSSEDRADLQSNLGEVVASMDRHFDKQAESVVEV
                     LKVLDSLHSYRLEDKES"
     gene            complement(26326..27090)
                     /locus_tag="PK522_01120"
     CDS             complement(26326..27090)
                     /locus_tag="PK522_01120"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNC40364.1"
                     /translation="MPALSGLSILVTRPAHQATYLVNRIKSLGGEPLLFPVLEITDVE
                     DVRPLLSIIDRLHEFDFAIFVSPNAVDKALPLICKNTPFPSHLKVAVVGKGSAQKLSS
                     YGIDKVILPAHRFDSEALLELEILKQVQQKRIVIFRGNEGRKLLGETLTNRSAIVEYA
                     ECYQRNKPKTDVSALLAYWSRGSVHAVTITSSEGLHNLFDMVGEVGQQLLKNTPFFTA
                     HERIAEIAKDLGLKDINLTESGDDGLIAGLLSYFNR"
     gene            complement(27113..28057)
                     /gene="hemC"
                     /locus_tag="PK522_01125"
     CDS             complement(27113..28057)
                     /gene="hemC"
                     /locus_tag="PK522_01125"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNC40365.1"
                     /translation="MPNSAAIPSKIVIASRESLLAMWQANFIKNRLRELYPQTEISIL
                     GMTTQGDQILDVTLSKIGGKGLFIKELEQALADGRADIAVHSMKDVPMNLPPGFVLAA
                     ITEREDPRDAFVSNQYKDLSELPAGSVVGTSSLRRESQLRARFPQLIIQPLRGNVQTR
                     LRKLDEGQYAAIILAAAGLKRLDLSYRITALLEPEQSLPAVGQGALGIECLDNRPDLV
                     RLMEPLHDRETAQCVEAERAMSRVLGGSCQVPLGGFAQITDNVLTLRGFVATPDGKRI
                     VSDELNGKPESGATLGEQLAQNLMAQGADKILAALVVS"
     gene            28189..30990
                     /gene="ppc"
                     /locus_tag="PK522_01130"
     CDS             28189..30990
                     /gene="ppc"
                     /locus_tag="PK522_01130"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNC40366.1"
                     /translation="MDVDAGLNDNSDEDSEEGKNTPLRHDIRMLGRMLGDTLREHEGE
                     ATFNLVESIRQTAIRFRRDGDPVAGQELDAILNRLKNKETIAVVRAFSYFSMLSNIAE
                     DLHHNRRRRIHLRAGSPPQSGSVTLALERALQSGINGKALEDFFDKAVVSPVLTAHPT
                     EVQRRNILDCQLTIQRLLTERDRTQLTPNEARHNEEAIRAAIQILWQTRMLRSVRLTV
                     HNEIENGLIYYSYTFLSQVPYIYAKIEDLLERRLGDQAPPVASFLKIGSWIGGDRDGN
                     PFVTHQVMLHAAERHSALVFDYYINEVYRIGQTMSLAERLIKASDELEQLAAASPDIP
                     ASRIDEPYRKAFLGIYARLIATSQRLGHAITPRNQVKAGESYADSAELVHDLDIIIHS
                     LKKNKSHWIARGALRDLRRAADVFGFHLAPLDMRQHSKIHEQVIADLFAQSVHKRDYL
                     QLTPSERQALLLDEITSPRTLYSPYFKYSATTQSELRILKMAAEIQHRFGHAALPNYI
                     ISMTTSVANILEVVLLLKEVGLLHSGENPRLGLNIIPLFETIGDLRGCGAMMDELFSV
                     PYYRKLLESRGNEQEVMLGYSDSNKDGGFLTSNWEIYKAEINLTEVFAKHQIELRLFH
                     GRGGTVGRGGGPSYQGIMAQPPHSVNGQIRVTEQGEVVASKYTDPEVGRRNLETLVAA
                     TIEATLLSHETIQADVTRYHQAMETLSSHAFSAYRNLVYETPGFKQFFLESTPVREIA
                     GLHIGSRPASRKPSDRIEDLRAIPWVFSWSLNRTIIPGWYGFGSAVEKFIQQHDQDET
                     GLKLLQEMYAKWPLMQTLLSNMDMVLAKTDLGIASRYAELVTDVSLREQIFSRIQEEW
                     QRSVKWLFQITGHSELLEDNPTLARSIQIRTPYIDPLNHLQIELLRRYRAGGDVEEQV
                     HRSIHLTINGVAAGLRNSG"
     gene            complement(31059..>31901)
                     /locus_tag="PK522_01135"
     CDS             complement(31059..>31901)
                     /locus_tag="PK522_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNC40367.1"
                     /translation="DQIDGGAGNDKLYGGLGNDLLVPDTGIIGNDSIDGGDGIDTINY
                     QRQGLISGVTVDLSLITSQNTGGAGIDLILNVEQVQGSDFNDTITGSNLDNWLNAGMG
                     SDIINGGDGNDAIFSGAGDLLGDSLYGGNGDDKIRGGTGNDVLDGGSGADLLRPNRGA
                     DSLTGGSDSDTFIFTISSSGTGSDSNPIAPDLITDFNGLEDTIILQSTEWSPNSVVFL
                     GNAPFTASGVSEVRLTYDLGLGTQRIEVDIEGNGSADIDSVINVIGTPIVADDLVFVS
                     TYPF"
BASE COUNT         9579 a         7285 c         6000 g         9037 t
ORIGIN      
        1 gcttgatgcc caacaccacc agcaatgact tgaccgataa tgggatccat actaggctga
       61 tgggcaataa aaccaatgcc ttcaagaaaa ttagcatcta aatcatccgt atgaatttga
      121 ctgaagtcca attttcttaa tgcggtttgc ccatgggagt ttactgtttc tactactaca
      181 accgggttac gatcaacagc ttgagttagt aacgcccatc gtgcatcttg ccaagttgat
      241 actgattcac cctcaatttt ttgaatgatc tcgccttctt caaaagcagc aaaagcagca
      301 ggtgttgctt gctttattgg acctatcact ggttttacac catttacacc tagcataaaa
      361 aggatccaat aaagtaaaat tgctagcaag aaattagcaa taggacctgc cgctacgatt
      421 gcaaatcgtt tataaacaga ctgctgatta aatgctcgtg gcaattcatg cggtaacact
      481 tcgccttctc gctcatccag cattttcaca tacccgccta atggaagcgc tgctaatacc
      541 cactctgttt ggtccttccc aatcgatttc ttgaataagg gctgtccaaa accaatggaa
      601 aatcttaaaa cctttacacc gctccagcgc gccacccaat aatgacccaa ctcatggaac
      661 gtaatcaaca cgcctaatgc aactataaaa gcgatgatag ttgttaatat cgtcatatcg
      721 aattagtcaa aaattgtata accttactag ccattcatta tacgcgcagg cagaaaacat
      781 gttacacctt gcacagtagc cattcttgtg ccgccaatcg cgcagcgcta tcagcagcca
      841 atacatcatc gagtgtatct atttcagctg agctcacact acccatgaca tgctcaatca
      901 tgtctggaat agcagtaaaa gatatctttc ctgtaaggaa agactcaacc gctacttcat
      961 tagcagcatt gagtactgct ggcatattgc cacctttagc taaagcctga taagcaaggc
     1021 gtagacaagg gaaacggcca agatctggcg cttcaaaatc gagtctagct atttcaaaaa
     1081 tattaagtga cttcacacca ctttccattc tatctggaaa accaagtgcg tgcgcgatag
     1141 gtgttcgcat atcgggatta cctaattggg caagtactga gccatcaata tattccacca
     1201 ttgagtgaat gacactctgt ggatgaataa cgacttcaat catttccggt gcagcatcaa
     1261 acaaccaatg tgcctcaatg acttctaatc ctttattcat cattgtcgca gaatcgacag
     1321 agattttctt tcccatgacc caattagggt gggcgcaagc ttgttctggc gtaacatgtt
     1381 caagttcacg ttcagaaaag cgccggaagg ggcctcccga agctgtcagt agtatgcgcc
     1441 ttactcctgc cgtagttaga tttccatcaa aatacttagg taacgattga tatatcgcat
     1501 tgtgctcact atcaatgggt aataaggtcg cttgattttg cttcacaata tcaataaaaa
     1561 tacgaccagc catgaccaac gtttctttgt ttgccagcat gactgattta cctgagcgtg
     1621 ctgcagcaat cgttggtctt atccctgccg caccgacgat tgctgccatt acgatatcga
     1681 cttcagggag tgacgcaact ttttctaagg actcaacacc ggataatact tgtgtgtcga
     1741 gaccaaccgc ttttagctta ttttctaacg ctgcagcgct tttatgatca agcattactg
     1801 catattgggg atgaaaacgt tgacattgta aaaatatttt ttcaacactt ttatttgctg
     1861 ttaaagcgca aacttgaaat cgatcagcgt gccttgccac aacatcgagt gtattctcac
     1921 cgatactccc agtcgatccc aatagggtta tttttctgac tgttgtcata atgttacgtg
     1981 tgaataaata agataaatca aggtggccgt aggtagcgtt gatgtcagtg catcaattcg
     2041 atccagaaca ccaccatgcc ctggtaaaat cgttccacta tctttgattc caacctgacg
     2101 tttcaattga gattcaaaca gatcgccgat gattcccaga atagccagcc caattacagc
     2161 aaaagtcagc ataataacaa agctgatggt ttcttctaga taataaccta gtgccattgc
     2221 atagcataaa accgcaagca aagctcccat aacaccctcc caagtttttc ctggactgat
     2281 ttgaggagcc aatttatgtt ttccaaactt ctttcccaca aaataggcgg ctgtatcaga
     2341 tatccaaact acgctcatga aaataaggag aatcattgga tcaattgttc ttaactgata
     2401 cattgccagt aaagttggaa gcaaaactaa ccatccaatt aacatcatca tcgctgtatt
     2461 tctggcgggt tttgataagg ttaacaagaa tggcacgatc aaaacccaga acactagcga
     2521 caccgcataa ggccacacga tgtcagttgt tataggattt acatccacca cttcacttaa
     2581 gacgaaaagt aattcaccac ccaatatcgt cgtgatgatt aaatacacca gcgtttgctt
     2641 agtattaaat tgacctagcg tacaccattc tcgagaagca acgattgcca ttcctagcaa
     2701 taacattccc cagagaatag gaggcaagaa aaataatgcg gatagaaaaa acagcaacaa
     2761 acaaattgct gtaatgatac gtaatttcag cataggcgga ctttaacccg ccaatataaa
     2821 tcaaaaatag ccgtaaacat cgatctaaac tttgtttttg atcgctattt taggccttat
     2881 tttatcaaca gaagtacgat cttgaagttg ctcgctagta cgaccaaaac gacgttccct
     2941 ctgttgatag gactgaattg ccaaatcaag ctcatgcgca ttaaaatctg gccataatgt
     3001 ctcagtaaaa tataattctg tataagctag ctgccaaagc aagaaattac taattcgaca
     3061 ctcaccacct gtacgaataa ataaatcagg ttcaggcgca taactcatgg aaaagtactg
     3121 cgacaaatct tcttcttcga aattttcatt tagatcacgt aattgaatca tcattttacg
     3181 aatagcctgc ataatatccc atcgcccacc gtaatttgca gctaccgtaa agatcaagcg
     3241 agtattattt gctgtgagct tctctccttg acgaataaac tcaattatct tagggtcaaa
     3301 ccttgataaa tcacctatca ctttaaaaca gatattattt tcatttagct tggtaacttc
     3361 ttgttcaagc gcacctaaaa aaagctgcat caagaactta acctcttcct ctggtcgtcg
     3421 ccaattttca ctgctaaatg cgaacagcgt taaataaccc accttgcgct ctatacatgc
     3481 tttgatcact gcacgaacag cttcaacacc atgtttgtgt ccagccactc ttggtaaaaa
     3541 acgtttcttt gcccatcgac cattaccatc cataataata gcaatatggc gtggagtatc
     3601 atttgttacc ggaatttcac gagtcgaact cggatttcgt ggcatatatt taaacagcca
     3661 ttaactcggc ttcttttgtt tgcaatagtt tttcaacttc aacaatatat cgatcagtta
     3721 atttctgaat gtcatcttga ccttttcgat catcatcttc agagattttt ttatctttaa
     3781 gcagctcttt taattgagca tttgcgtccc tacgcgtatt ccgcattgca acgcgtgctg
     3841 cctcggcttc attttttacg attttagtta aatcacgtct tcgttcttcg gtcaacggtg
     3901 gcatgggcac acgtaccata tcgccagtag tcattggatt caaccctaga tcagaatctc
     3961 taattgcttt ctcaatcgga gataacattt ttttttccca aggcgtaaca ccgattgttc
     4021 gagcatctgg caatgttata ttagccaatt gactaatggg agtcggggta ccatagtaat
     4081 cgacaagaac ctgatccagt aatcctgtgt gaggtctccc acttcgcact ttaccaaagt
     4141 cggttttaag tgcttctaaa cttttctgca tcttctgctc agcagctttt tttacatcag
     4201 cgatcatatc aagaaatctc catcaatcat ctattattga ccgaagttcc ctcatcctct
     4261 cccaggagta ttctttttaa cgcacccact ttaaaaatgc taaaaacccc gattggtaaa
     4321 ttttgatccc tacataatgt caatgcagtt gcatccatta cctgaagatt ttttgtaatt
     4381 gcttcatcaa aggataaact tttgaatcgt gttgcatcag agttcaattt gggatcactc
     4441 gaatatattc catcaacttt cgtggcttta agaacaatat ttgcatttat ctccatacct
     4501 cttagagccg ctgcagtatc cgtcgtaaaa aatggattac ctgttcctgc agcaaaaatc
     4561 accacttttc cttcctctaa ataacgaatc gccttattgc gtatataggg ctccacaact
     4621 tgatcaatcc gcaatgcgga ctgcactctt gatataattc cgctacgtga taaagcatct
     4681 tgcaaagcca aagcgttcat aagcgtggcg agcattccca tataatctgc ggtcactcta
     4741 tccataccag cacccgccga cttaacaccc ctaaatatat tccctccgcc tacaacaatc
     4801 gctatttcaa cacccagttg gatcgctccg gttatctcaa ttacaatatg ctcaaccaca
     4861 tctcggttaa ttccatactt atcatccccc attaaagctt cgccagaaag ttttaataaa
     4921 atacgcttat aagtaatggt tgccattggt tggtttaaga tcaactctta ttttctttca
     4981 tttgacttac ttgtgccatt acttcagcag caaaatcacc tgatttcttc tctatccctt
     5041 cacccacgac gaaaagtgtg aatgcatgga ccttacctga ttttgcagcc aaaagcttct
     5101 cgactgtctg atctggatct ttaacaaaag gctggcccaa taatgtcacc tcagcaaaat
     5161 attttgaaac cctaccttca accatctttt cgatgatatt agctggttta cccgattctg
     5221 cagcttgtgc tttaaaaatc tgcctttctc gctccactaa atcatgagga acttgatcac
     5281 tagaaacgca tagtggttta ctcgcagcga tgtgcattgc tagatcttta cctaaaacct
     5341 catcttcaca tgagtaatca atcattaccc caatttttgt accatgtaaa tatgtcgcaa
     5401 gcttaccttt tgctgtataa caagcactgc gcctaatact aatattttca cccaatttca
     5461 ttacaagcga ctggcgaaaa gcctctacgg tttctccctc tggcaaaacc atagaactta
     5521 attcatcagc attattaact tttttgtcag ccaccagttg cgctaaagct tttgcaaaat
     5581 tgatgaaatc agtatttttc gctacaaaat cagtctcaca gtttagctca accaaaccac
     5641 cttgcttctg atcggccgaa acatatgcgc caatcacccc ttctgctgca atacgcccag
     5701 cggccttact tgccttcgct ccactcttaa tccgcaaaag atcttcggct gctttcatgt
     5761 cgccgttcgt ttcgaccaaa gcttttttac attccatcat gcctaagccg gttagctcac
     5821 gcaaatcttt taccatacct gcggtaatat ccgccatatc tactccacaa acccagaatc
     5881 aaaattattt atagaaacta taccaaaaaa taccctctaa ctctcagcag cctctccact
     5941 catttcaacg atttcctgta tcgattgctc tcgaccttcc agaatcgcat ctgctgctgc
     6001 acgtgcgtaa agacgtattg cctgactcga gtcatcatta cccgggatca aatattgaac
     6061 tccagcagga ttatgattgg tatcaacaat accaactact ggtataccta gtttattagc
     6121 ctcgacaatc gctccttttt gatatccgac atcaatgaca aacatcacat caggcaaccc
     6181 tctcatgtct ttaataccgc caaggctatt attaagcttc tcgagttgac gctgaaaatc
     6241 gagaccttcc ttttttgtta acttatcgag tatgccattc tgaacggagg cttccatatc
     6301 ctgcaagcgc tttatcgact gccgaatggt tttaaaatta gtgagtgtac cgcccaacca
     6361 gcgctgatta atataaggag aaccacaacg aattgcttct tcagaaagaa tatcgcgagc
     6421 ttgtcgtttc gtacccacaa acagcactgt tccactcttt gccgtaacat tacggagata
     6481 ctgcatagcc tcattaaaca tcaccaatgt tttttccaaa ttgatgatat gaattttatt
     6541 tcgctctcca taaatgtact gagacatttt aggattccag aaccgagttt gatgaccaaa
     6601 gtgcacaccc gcttccaaca tttgacgcat tgtaactgac atataaaatc ctctagggtt
     6661 agtcctccac ttgcctcata tcatgattgc ttagcaccct acatcgacaa gtgtgtgtat
     6721 ttaaataaat cgtaagatag atcgcgacta gtgtcggatc tatctaaggc tgcattatag
     6781 cacgcctctg ttgacactca agaaagcgca aaactttaat ttagctagta acgaacaaat
     6841 gaacgttggc ttcaatcacc ctaatagcta ctaattttac gagtcaggcg atggatatca
     6901 tgcaattcta catgcttatt attaatggta atcatccctt catcctgcaa cttggagaat
     6961 gagcgactta cagtttcaag cttaagaccc agataactcc caatctcttc tcgcgtcatt
     7021 ctcagaacaa agctagtcgc agagtacccg cgctttacaa atcgttctga caaattgagc
     7081 aagaatgctg ctatacgttc ttctgctttc atattaccaa gcaaaaccat cacgcgatga
     7141 tcttgaacaa tttcccgact catgatttta tgaaactgat gcatgagtaa aggaatactg
     7201 cagctaattt cttcgagtct ttcaaatgga acctcacaaa cctcactatc ctcaagcgca
     7261 acagtatcac acgtatggat ttctgcactg atggcatcca ggcctagcag ctcccccgtc
     7321 atgtaaaaac cagtaacctg ctgacgcccc tcagaatcaa ggatataaga tttaaaaaaa
     7381 ccattcctga cagcataaag ggatttaaat ctcataccag cgtgcaaaag atattcccca
     7441 cgctgaattt tatacttatg atcgatcagg tcgctcaaaa cctctgtttc atgcgcatcg
     7501 agaccgactg gcaagcataa ctcacgtagg ctacacgatg cgcatttgga tattgcctga
     7561 gaggcaatat atttttttag agctgatatg ttattactca aggttctgga tagttagtaa
     7621 ttcgaactca gaaaacagtg atattttttt attttgttac actattatct aggatatatt
     7681 gcgcgagccc ttccgctgtt tcacccgtca aattaggaaa cgctggcata ccaaaacctt
     7741 taccattttt gattctttca atgattgctg cggtattagc catctcggga ctcagcgcca
     7801 ttactttccc atttacgggg ctatggcact tcacacaagc ttctttaaaa atactttcgg
     7861 gtgttgcgct cgcactaggg gtatatttat ctgaacaacc acttacaagc gctaatacac
     7921 caaccatgct taacaaccat aatttttttt tcatgcgcct tttcctccct tgaatatctt
     7981 tatttattta acttaactga ttcgttatac attttaagtg tatcacaatg aagtaaggat
     8041 attaccttct gaaagcaaca cgctccggtt ttgaatagcc aagcaacgta atacactaga
     8101 attcggtgtg tattaatttc attaaaacct aatactagga actaaatagt attgttttca
     8161 tcgcttaact ttagaaaaga ttcactaaac tagcgaaaag ttgctcaaaa aattctcaaa
     8221 taattaaacg agaatttcgt cggggatctc agcaatgctc aaattggttt tgctggtatc
     8281 aacttattaa caaagcgaac ctagagctat tatgatttaa gaaaccagtt atcgctatac
     8341 tgcattcgta ctttatgcat atagcaagat tttcctattg gtacacatga aaaattcgaa
     8401 aacactaaca gaaattgaaa ttgctcgaga agcattacgc cagctcgctg taaatcgcaa
     8461 attaccaacg cccgataatt accgttcgct ttattatcaa attgctgggg ttgagataat
     8521 tgatttcttc ccagaaaaaa cattaaaaat aatcatagaa aaattacctc gcaataattc
     8581 tgatcaacaa cagatcattc agtcatttga agctgcagta tcaaaaggtg attgggagag
     8641 tttgggagca atactgatcg atttattaag cggtaatgtg attaagcaaa atccaatttc
     8701 aaacgatcat tctaatgatc atcaccttga aaaagataac gtacaaccca atgaaactgg
     8761 caaattaatt acatcgatcc tccctaggga attacaagac caattagggc gtttatttgg
     8821 ggactgttta agtatattgc tatctacctc accagaaatc tcaaaggatg ctgagtcact
     8881 tgctagggaa gttcgagtta tctctgaatc tgggcagctt tcacagttta ccgataaact
     8941 taagcaattt aactatcaac tgcaatatgc tgcagaagac cagggcgaac tcagaacttc
     9001 gctcttaaaa ttatttcaac tcgtaatcga taatatcagc gaattagtcg ttgatgacgc
     9061 ttggctccaa ggacagatcg ccattgtcag agaaatgatt actgagccgc tcgacttaag
     9121 gttaattgat aatgtcgaac gacaaataaa ggaactcata tacaaacaag ggcttcttaa
     9181 aaaaaatctg attgataatc agtcccgact gaagctcatg ctttcatcct tcgttgatcg
     9241 gttagcggag tttacacaaa ctaccggtga ttatcacgct agtctaggtg tttatgctga
     9301 agaaattagc caggcagaag atatttcaga actttctgat gttcttgaca gaatcatcca
     9361 gcaaactcga caagttcaat cttcagcaaa acgctcccat gatgaacttt tagaactccg
     9421 ctcccgtgct gagtccgctg agaaagaagt cgatcgattg caatctgagc tcctgcatgc
     9481 aagtgaactg attcgccacg atgcattaac gggcgctctt aatcgcaagg gtttggaaga
     9541 agccattgag cgagaagttt ctcgaaagta tcgtgacggc tcaagtttgt gtattgcatt
     9601 actcgatatc gataatttta aaaaattaaa cgatacacat ggccatgaag cgggcgatga
     9661 tgcgttagtc catttagcag cggtcgttcg caaatccctt aggccccaag ataccctggc
     9721 acgttttggt ggtgaggaat ttgttgtttt actaacggga acaggggtta acgatagcgt
     9781 aaccgttatg cagcgtgtcc agagagattt aactcgctgc tttttcctgc atcaaaatga
     9841 aaagatattg attaccttta gctgcggtat cgctgagctc gctgaaaatg aagatcccaa
     9901 aaccacgatc aagcgtgctg atcaagcaat gtatctagcc aaacgatcgg gcaaaaatag
     9961 agtactcgca gcatgacatt aaggaatttg cttttcagct agggtgatgt gcgcaatata
    10021 ttctatttct acctgaattt tttgctttat aaagcgcacc atcaacgcgt gataatatat
    10081 ttttaggggt tttatcatcg atctcacttt ggattatccc catacttaat gtgatcgaaa
    10141 gactaacctc tttgatctga gaatttacat ttatagctac ttgagcacga atcctttcag
    10201 cggtaattac agcagatgct gcgtccgtct cagtcaatat tacaacaaat tcatcaccac
    10261 catatcgcgc cgcaaaatca acacttcgga ttgatttgct aattgcttgt gagactgata
    10321 ccaaaatttc atcgccgatg acatgaccgt aattatcatt taatgttttg aaatgatcga
    10381 tatccatcat tacaaccgaa aatggtcggt gatttcgttc aaatctcgct atctgatcaa
    10441 taagtatagc gtcaagcttg ttgcggttat acaactcagt caggctatcc gttattgaaa
    10501 gaacttttaa ttgctggttt tgcttttgca ttgcttcatg tgcagccaat atttcagctt
    10561 gactacatct taagttctcc gtcatcttgt taaagctcgc tgtcaactga ccaatttcat
    10621 ccgctttaat ttcatttatt tgtacattaa gatcaccatt taaaatacgt ttcgttcctt
    10681 ctatcaaata gctcagcgag gccacgatag aatgaccaat ataaaaagcg attccgccca
    10741 cgatcacaat tagcaaacct actaaactca taaatagatt acgtaactcg atccaggcag
    10801 caatggcttc agcatagtct ctttctacta aaacgataat aggggcctct ttcgattttg
    10861 agattaaccc gattaccttt gaatgagtaa gtcccttaaa agccaaggcc tctccaccgt
    10921 aatcttgtaa ctgctttaaa gttttcgaat cgagcgaagc tgaataattt atcttgtgat
    10981 tactactcag aattgtattt cccgcgtacc ccagcagagt gatttctcct aaagaaaaat
    11041 cggtattatc aactaaacta ctcatgaatt tttccatatt cagcgttgct aatattgagc
    11101 cgatcacttt ctcatcatag gagagaactg gggagtaaag ctctagaaca gcggtatcat
    11161 atcgatcact tcgatagggt ggcttaattt tgatattact cgtaagcata tcgtgctgct
    11221 cattaccgcg aataacacta tttagagact ctggtatgct acttgcaaca acatttccat
    11281 ttaaatcaaa aagagttaac tcaacgattg ttggaagctt ttcttgaaac gaacttaaat
    11341 attttgataa aacaagttgt ctcgatccct ggtggttgct acttttgctc acagaatcac
    11401 gagctgataa agctaactca ttgataatca ggttagatac agaaagtaca ctgacggcat
    11461 ggtaattttc attaatccat aaatctaatt cgcggctaac attactagca agtacccgta
    11521 attcacgagt cacatgattt ctcactagcc cttcattttt aaaataggag agtagtccga
    11581 gtgatagcga tggaattgat attgcaatta ttgcgaaagt aacaaactta gttttaatac
    11641 ttttcaatct gtttcctaag aatagcgcta taaagagatt ttattctcta gcaacacaaa
    11701 aaacaaagtt tactattaca aatcagagat actgggcagg attaactaag ctatataaat
    11761 tcagatccaa taatcgtaca aagcagttaa aaacactgca taactttttt caagattatt
    11821 acatccagtc tttacttctg ctatcttgct ctagtttaat gatgtaacgt tgaatcatca
    11881 ttcgagtttt actgggcaaa tcaataaact cacacccata tcgggtataa atttgattac
    11941 ttttagaagg catttcagaa atattcctaa tacgaattgg tgtcttaata aggccaattg
    12001 ggctagcaag ttctatgctg caatcaggat aaatctcgcc aacttcaagc ttttttatat
    12061 taggacggtc atgtttgatc gacatccctc cacaactgat atcaagtatc gtaaagccaa
    12121 tcacctgttg gtctttatcc aaaatagcac accttggttg ttgtttcata agcggcgaca
    12181 tccgaaaata ttctcttcgt tgtacacgtg aaattacttt cggaaaatct agccgatagg
    12241 cagcatctct accaaaccaa gttttcccaa tcgagaaact ttcgaactca actcgaatac
    12301 cattatgtgt tgctacacag actaatctct ctgcaagttg ggctcgctgg ttaaaatatg
    12361 cttcaggata ataacctaca atcaactcat cttgtgaagg gttaatattt aaaactgtgg
    12421 ttggcactgg actgatattt tcaccaataa gataaagcaa aactttagtc tgttgctcta
    12481 ggaacgttct cagaatatga gtaacctcat tctgggaatc aatcaaatag tcttggttaa
    12541 tattcattca gatcagaagg gcatttattt aagatttata cttgcatgtt cataatttcc
    12601 tgataagccg ttaccaatct atttcttatc tgaatcatcg actgaaacga gatgttggct
    12661 ttctggagtg aaatcattac atcgtgcaaa ttagtatcag atccaccttt cataaactcc
    12721 tgactcaatt gccctgcggt ttgctgagta gcatttactt gatcaaccgc tgatttcaga
    12781 atattactaa aatctgcctc cttgggatta aactgagcat tatctggctt taaggaacca
    12841 gaaattgcat cagaagtaag ccttaattga gttaagattt ggttaatatt agatttatcc
    12901 actacagccc tcctatcgat tctcacccaa ctaggcgata ttgttgcaat ttataacgta
    12961 atgtacgctc agatatcccg agcctctgaa ctgccaattt acgcgagcca tttacttcag
    13021 caagtgttct aagaatatgc tcacgctcta aagatttaat atccttcgac tgagatagat
    13081 cactcgtgtc gaacggtagt gtcacatcag atcgcgcttg ctcgggtaaa tttaaatgca
    13141 ttacatcaat ctcatctgtt gctaaaatca ttgcacgttg aataacattc tctaattcac
    13201 gaacatttcc tggccaagag tattgcctaa gcgcaagcaa tgctaatttg gtcaaagagt
    13261 aatttcgccc ctttattcct ttattcggac tctgaagtat tcgatgaacc aaaggttcaa
    13321 tatccagtgg gcgttccctc agcggtgcga tttctaaagg aaaaacatta agtcgataat
    13381 ataaatcttc ccgaaaagcg ccttgcttta ctaaagaaaa aatatcacga ttcgatgctg
    13441 ttaaaaggcg aatatctagc ttaacgggct ttctaccacc aattctctct acttcacgtt
    13501 cttgaagcac tctaagcaat ttagcttgca aacctaacgg catttcagat atctcatcca
    13561 gcaataaagt accctcattt gcctgctcaa acttaccggg ttgagcctga gctgcgcctg
    13621 taaatgcccc cttttcatat ccaaataatg ttgcttccaa taaattctca ggaatagcag
    13681 cacaattgat agccacaaat ggtttgccca aacgcggtga atgctgatgg atataacggg
    13741 ctaaaacctc cttcccactg ccactttcac caattaacat gacagttgca gaagactctg
    13801 cgacacgctt ggctaaagaa aacattgcgc tagttcgggg atctttagcg ataacttcct
    13861 cataattgtt gcgttgatta ttcgcaggta ctaaataatt cttaatgtag gtcagcaaat
    13921 aatccggtga aaagggtttt aacaagtaat cgctggcacc tgcccgcatt gctgcaatcg
    13981 ctttattaat ttcaccataa gcggtcatca gcagcacagg gatatccgga taagtttcct
    14041 taattctttt caacagtata ttcccatcta tgggttgcat ctgcacatcg ctaattacca
    14101 atccaatctg ttgggaatta agtaaatcaa gcgctgcagt gccattcgat gcccctaagg
    14161 ctttataacc agacaactta actgtcgtgc atattgcctc cagtaaatcc tgatcatctt
    14221 ctacaatcag aatcgatagc tcattcattt caataatcct ctaaatagaa ctggatagat
    14281 ttgaattggt aatattgctt aagctagttc ttggcaatcg gagaataaat tcactacctc
    14341 gatcagtaag taactttact tgcacatccc cccccatttg tttgattgcg ctatgaacga
    14401 ttgctaatcc cagaccggtt ccttctgggc gtgtcgtaaa aaaaggctca aatagccgct
    14461 cttcgtttgc aatatcaata ccatttcctt gatcagctac tgtaaaaaac acttcatcgg
    14521 tagttaagtc acaagttaat gaaatacgtc cacccacggg tgaagcttgc atcgcatttt
    14581 caagtagtgc tattagcgag ctacaaagtt cttcataatt aacggttaaa gctgccccct
    14641 caccaccatc tttaacaaca aactgcaaat tcttttttag catgtggggt agcatcaggc
    14701 tttgaatatc ggataataaa actgagatat ctatcacttc tttttttact aactcacctt
    14761 tcacgaaccg caacatacta ttaatcagtt gatttaaata ctgcaatcgc tcaattgttt
    14821 ttacggcaaa gctgcttctc tcctgagttg gtaactcact attgctcaag tgcgtagcat
    14881 aaatcaatgc cgttgaaagc ggggtacgca attgatgtgc taaatttgct gccattcttc
    14941 ccattactgc aagtctttgt tcgcgcttga tccgttcttg atttgtatag gcttcagtga
    15001 tgtcttgaat taataaaacc attttccccg agtcttccga ggcactgctt tcaattgaaa
    15061 tacgacgaaa atcgctatgt gtttcttttc ttaaattcca ctcgttagca acatccgttg
    15121 ccaataaacg atcttcgagt atctgctgcc aggtacattg caataacggc tcacctagaa
    15181 tagacagagc aactggattt gcactttcaa taacaccttc attatcgata gcaacgaccc
    15241 cgcccggtaa agctgacaac aacaaactaa gctgttgaga aagattctct ttttgtgcaa
    15301 gttgatgttg taactcgccg ttagccaaag ctaattcatt cgttaaatgt tcaacttgtt
    15361 gctgcaaatc gcgataaaca gttgaaagct gttcggacgc ttgactaaaa acagtaaaag
    15421 catgcatcag ctgatgatga tcagcatctg attttcctaa tcgaacgtta gattcagctt
    15481 tcatgcttac aaaatttaat ggtttttcca gatgttgtgc agcttagcat tagccacctt
    15541 gcacatagag atgaaataaa gctagcaaaa ttattctgtt caatcattca gaagtaataa
    15601 aaatcctgat aatcgcatta tcgaatttaa gttaccgata cttcatttta aacagcatct
    15661 atattcaata tttaacacat tgattggtaa ctaaaagtag ttaggaggat tgtgcgtggc
    15721 tacagtatca gaagaaattg gaaccaaaac aaatcgattt agcgctattg ctcaattatc
    15781 agggcaaaag aaactaggtt taatgctagc agcggcggct agcattgcat tattggttgg
    15841 aatcgcaatc tggagtcaga gccccgatta tagagtgcta tacactaata taaccgagca
    15901 agatggcgga gctatcatta atgcattaca gcaaatgaat ataccttacc aattctctga
    15961 aggtaatgga accattttaa ttccaggaac acaagttcat gaggttaggt tacgcttggc
    16021 aagccaaggg cttcctaaag ggggtatctc cggtttcgag attttagaaa atcagaaatt
    16081 tggttccagc cagttcttgg aacaagttaa ctatcaacgt gcattagaag gtgagttggc
    16141 gcgttctatt cagtcccttt ctgctgttca aagtgctcgc gtacatttag caatcgctaa
    16201 gcccaccgtc tttgttcgag aacgtcaaca acctagcgta tccgtattac taaatttata
    16261 cccaggaaga gtgctaacca cagaacaggt aagcgctatc attcatttag tttcaagtag
    16321 tattccaagt cttccagtaa aaaatgttac cgttgttgac caaaatggca atttacttag
    16381 cactcccaaa aaggaaggaa atgaaactgg cctagacgac agtcagctca aatatattca
    16441 tgaattagag caaaacatta ataagcgcat tgaaacaatt ttgcttccaa taactggtcc
    16501 caacaatgta cgggctcaag ttactgccga cgttgatttc tctagaacgg aacgcgcaga
    16561 agaaatctac aagcctaata atattgcaac cgaggctgct tcgattcgga gtcaacaaaa
    16621 atcagactcc atgtcagcta atcgacccga gagcggtgta ccaggagcgc tatcaaatcg
    16681 tccacctgct gctgctgagg cacctgtaga tgtcgcagct ggtgaagggg acacaaaaaa
    16741 tcaagtaccc gttgataccc gctccgaatc aactacgaat tacgaattag acaaaacaat
    16801 tcagcacaca cgtcaatcag tgggacgtat taaccggcta tctgcagccg ttgtcatcaa
    16861 ttatcgcgga aaaatggatg aagcgggaaa cttgatacat gaaccacttg ctgcagaaga
    16921 aatcgagaaa attaatagtc tcgttaaaca agcgatggga tttgatgaaa atcggggaga
    16981 tacacttact gtaaccaata gtcaatttaa tattctggaa gaagaaattg cagaaattcc
    17041 tttctggaag caaccagata tgattttgct tgcaaaagaa attggtaagc agcttattat
    17101 tgctgctttg gttttattct ttttgcttaa aatattaaaa ccattcctaa aaaccttgtt
    17161 gccacccccg ccacctcctc caccagcacc gcttcaggca accgctgggt tagaagagag
    17221 taataactta gcattgagcc atcagcagga agtagttagc catgagcaga atataactag
    17281 agctaagcaa ttggctttaa acgaaccaat catagtagca aatgtgatca aagaatgggt
    17341 atctgacaat gagcgatgac ggtattctta aaagcgcaat tcttctcatg tcattaggag
    17401 aagaagaggc agctgaagtt tttaagtatc ttggaccgaa agaagtccag aaattgagtg
    17461 aagccatgtc aagtttaaac aatgttaaac gtgacaagat tgagtccgtc gtacatgagt
    17521 tctgtacgct agcaagtgat agaacttcgc tcggtcaaaa ttcgctagca tatattcgta
    17581 atgttctaat ccgcgcttta ggggaagaga aagcggctag cttactggat cgaatgacgg
    17641 aaaaagggaa cgatactagt ggtatagaaa acctaaaatg gatggatact gctgcagtag
    17701 cagagttaat caaaaatgag cacccacaga tcattgcaac catattggtt catcttgaaa
    17761 gagatcaggc cagcgcaatt ctggcattat ttacggagag gttacgcaat gatgttattc
    17821 ttcgtatcac aacccttgac agcattcagc ctactgcact gcgtgaattg aatagtgtat
    17881 taaataaatt gctatctgga agtgacaata ttaagaaagc gcccaaaggg ggaatacgca
    17941 cgacagctga aatgctaaat ttcattccca gttcgctaga aggcaatatt atcgagcaca
    18001 taacacagta tgatgagagc attgcacaaa aaatcatgga tgagatgttt gtatttgatg
    18061 atttactgac tgttgatgat caaggtatcc aactcttact cagagaagtt cagtctgatt
    18121 cactgatcat cgctctcaaa ggcgccaaag aagaattacg taataaaatc tttaagaata
    18181 tgtcacaacg ggctgcagaa atgttgcgcg aagatcttga aatcaaaggc ccagttcgtg
    18241 tttcagaagt tgaagctgcg cagaaggaaa tgttaaaaac cctccgacaa ctagcagatg
    18301 atggaaaagt agcgcttgga ggcaaaggtg gcgatggttt tgtataacag ctaaagatag
    18361 ctctcaatct aacaaactac cgtgatcatg gacctacacc tgctaattca tatcgaacta
    18421 ttggataagg ataaacatac aaatgaattc cccatcggtc attcctaaaa agcagctttc
    18481 gtcctttcag aaatgggagt taggttcatt tgaaacaaat ttagcaaaca acacaacaca
    18541 attagatgaa agcgtcgatc aagttgctaa agaaaccatt tcacttccta cagcagaaca
    18601 aatcgaagcg atctatcaac aagcaaagaa tgaaggctat caggcaggct accaagaagg
    18661 caagcaacat gcgactgtag agatggaaac cactattcaa catctccaag tcttaatggt
    18721 caagtttgag gaggagcttc agcacattga tcaaaccgtt gcgcaagagc ttctcgaact
    18781 cgctatcgaa tttgcgagaa agatcgttac tcaatcgctt gaattaaaac ctgagttaat
    18841 cattccaatt attcaagacg ctattcaaca cttgccatct gcgatgcagc cgcttcatat
    18901 catactgcat cctgaagatg cgacgctggt tcgcatccaa ttggagaatc agttatcgca
    18961 ttcaaagtgg aaaatctacg aaaacagcca gatagaaagg ggaggatgta aagtagaagc
    19021 gggagggtgc gagatagacg ccacactcgc ttcgcgctgg aaaacaattt tatcaaaagt
    19081 tttgctagaa aatcatcaac cgaattaggg taagcatgaa ccattgctcc tcatggcaaa
    19141 gttttctaaa caactgcaag caaattgtca gtcactcaaa ttcactcatt attagtggta
    19201 cattgacccg tgtagcgggt ctagtgatgg aagcggtagg attgagactt gctgtcggca
    19261 gcacttgcac cgttttcctt cccaatggcc ataatgtctc tgctgaagtc gttggattct
    19321 caggagatcg cctatttcta atgccttcta gtgatgttta tggactagtg cctggggcga
    19381 aagtagttcc caccgaatac gcgagtgatc atcccagaat cggcaaaaac tggcgcccaa
    19441 gaaggcgagc tgccgaccgc acaaagcaaa ttaatgttgg atatgaacta ttagggaggg
    19501 tactgagtgg tgccggagag ccccttgatc aattaggtat tatccatacc gagcagacgg
    19561 ttcctttata tagcagacca ttcaacccac tagaaagggc ccccattgat atgccccttg
    19621 atgtaggcat tcgcgctatt aatacgctac tcagcgtggg ccgaggccaa agaatgggac
    19681 ttttcgcagg aagtggcgtt ggtaaaagtg ttttgctcgg aatgatggca cggtacacca
    19741 aagccgatgt gattgtcgtg gcgttgattg gcgagagagg cagagaagta aaagaattca
    19801 ttgaaaacat actcggacaa gaagggctcg ctcgttcagt tgttatcgca gcgccagctg
    19861 atacctctcc ccttatgcgc ttgcaaggtg cggcttatgc aactgctatc gcagaatttt
    19921 tccgtgatga gggaaaaaat gtactactca tcatggattc gctaactcgg tttgccatgg
    19981 cacagcgaga aattgcatta gcagtcggtg agcctcccgc cactaaaggt tacccaccat
    20041 cggtatttgc gcgtttacca caattggtgg aacgtgcagg aaatggtaga caaggtagtg
    20101 gctcaatcac tgcattttat accgtcctat cagaaaatga tgatcccaat gatccaatcg
    20161 cagacaatgc ccgcgccatc ctcgatggac atattgttct ctcacggcga ttagcggatt
    20221 ctggccatta tccggcaatc gatatcgaat catcgatcag ccgtgttatg ccgcatttag
    20281 cttcacaaga acaattggaa tggacccggc gtttcaagaa tctttatgcg cgttatcacc
    20341 gcagccacga cttaattagt gtcggcgctt atgtcccagg aagcgatccc gagcttgatt
    20401 tagcaataaa aatttatccc gcactagaat ctttcctcca gcaagggatt ttcgagcatg
    20461 agaacattgc agaaagcctc caaaaattat ccaagctact cacacttcgg tagctcgctt
    20521 ttgaatcact acccatagta aatagcaaac cgaatggcaa ctacccaatc tcttaaaacg
    20581 cttgtcgagt ttgctcaaca aaaaatggat gcttcagcca aaaagctcgg atttcttaat
    20641 tccaaacagc aagaagctga gaaaaaactt aatctattga ttgattaccg acgaaattat
    20701 caagcccatt ttgtcaatac aaaaaacact ggaatgaatt caattgagtg gactaactat
    20761 cttgcattta ttggcaaact ggatgccgca atcgtcgagc agcgcaaagt aaccatcaat
    20821 gcacaaaaag aaacagagat tgttaaaaca gagtatcaag attctcgccg taaactaaag
    20881 tcctatcata cgctgctaca gagaattaaa catcatcatg ccatcaagct tgagaagcat
    20941 gaacaaaagc tcctggatga gtttgttact attgccaggc aacataaaac agaaggctag
    21001 gccccaagaa aatctgatcc tggcataaca attgcatgag gatagatatt aatttagtaa
    21061 ctcacctatt agccatgtta aatcttacaa gcattcagga aacattactg ccatctgtta
    21121 atcccgacat taattcaccg ctcacggaca gcgctggtct aaatcaatct aactcgaccc
    21181 cattccaaga gatattggga aaaaccctag cagaagatct aaaaaccgag cttagcgccg
    21241 acattactga cactgaaaca gccatatcga atgagttact tattgaagca accaacgcag
    21301 tcgaaatacc tatctccttg atggctgaaa caaacaccgc taacagcaat tcaatgacta
    21361 gcttctcatt agcagattct tccaatatcc tgaggggggc acaaaatcaa atcaatccat
    21421 cgctactcaa tacgatagaa gaaaatattt tacaaatcac gccactcctt gacacagcgc
    21481 ttgaaaatag cgatgcgcca ttggcaacgc taataagtga taatttaaag acgaccattt
    21541 ctactagctc agcaagcaac cagacttaca caactttttt gcaagcccaa gcttatcaaa
    21601 acaatagtat accgagtata aatacttcgc cctactctgc tctgaatcag ttcacccaat
    21661 tattaacacg tgacgcttca tctgaagaag tcgacatcac cccggtaaat tttgccgaga
    21721 tcggcaaaat tttgccgatt tctcccgatt cttctttggt agcacaaagc aacttgataa
    21781 ctcaaacggt gtctccttcc agctcaactc ccttattacc aattcatcca caccatgttg
    21841 cgactgaatt tggcaagcca gcatggggca acgatttcaa tcagcaaata acctggttag
    21901 ccacccagaa gcatcagatc gcggaattac atctacatcc ggccgaactc ggtccggtag
    21961 aggtcatttt gcacatcagt aacgatcaat cgactcaggt atctgctcaa ttcacctctc
    22021 cgcatcttgc ggtacgcgag gcaattgaat ccgcgctacc tcgattacgt gaaatgatgg
    22081 cagaaaatgg aattaccctg ggaaatacca cggttggtgc tgaaacatct caacaacatg
    22141 ctaatcaaca gcatggttca attgcttcaa atgccaatga aaatagcaca acaacagtag
    22201 caacaattca gggattatca accgaattta atggcagtgt tcttccaagg catcaaggta
    22261 ttatcaatac atttgcatga ctcgaaacac ctttgagtag aattgaggaa tagccaagct
    22321 cgcccgctca aacaacctga tagcacggta tttcaaatta acgcaagcaa tttttatcgg
    22381 ggatcacctt ggatattcct tcatgcacag ttggataaag caatttgatg cgcaactctc
    22441 gtttcattcg atcatttgtt aaacgccgtg attcttccat gaaagatagc aggccggctg
    22501 gaatctgttc tttggcttgt gtacgcgaaa ttctaggtgg gtggggtaat ttaaaagtgt
    22561 ccgcaaccaa atcaaaataa tcacccattt ttaaactcga atcatcacat gcgttataaa
    22621 ttctactatt tttcccataa cggaaagcag ctacgataat ccgcgccaga tcatcggcat
    22681 ggatgtgatt agtataacta tcttccttct ctagtaatgc tggagtacct tgctttagcc
    22741 gagcaatggg caatcggtct atagcgtaga tgcccggcac ccgcaatata gtgacattgc
    22801 tacctgtatc ggtgccccat ttcctaatct gacattctgc atcaaatcgc ctccaagctc
    22861 gatcattacg tggcttaacg ggattatgtt ctccaaccca ctgcccatcc ctatcaccat
    22921 aaactccact tgtgctgata tacactagcc gatgtggtat ccttcccttt gctctggaat
    22981 gttgtcgact cagcgctgcc aaaagatgcc gcgtacgagt atcttttaaa cccatattag
    23041 gaggtggcgc cagatgaata atggcatctg caatcccaga caaccgcttg aggcttgctt
    23101 gctgatccaa atcacccaac aatggggtga tttgatgtga acgtagttta ctaaaatttt
    23161 ctgtccgacg agtcaatcca agcaatcggt agtacctagc gagaagcgga gctgttctga
    23221 gtgcaatgtc cccacatcca ataatcagta acgttttttt catttcagcg tatcaaatta
    23281 atcgttttaa atttttaaac tatgtcttat caagtcatcg tcaagccaag caatcatact
    23341 tttactatcg aaacagggga atcgattctt caagcagctc taagacaagg catttcactc
    23401 ccttacggct gccgcaatgg ttcatgcggc gcttgcaaag gaaaaatttt gcaaggacat
    23461 gtcgattacg gtagatatga tgaaacaaca ctcactgaac aagacaaaca aaatggcttg
    23521 gctttgtttt gttgtgcatc ggcattatcg gacaacttag tgatcgaatg tcacgaggtc
    23581 gataccctta aagacatcag aatacgcacc atgccctgtc gggtccacaa acttgaccgt
    23641 gtagcacctg atgtgatggt tatttatctc aagcttccta caaatgaacg tttgcaattc
    23701 ttgccaggtc aatatatcga tattttactc aaagacggca aaagacgtag cttttcatta
    23761 gcaaacgctc cccacgacga tgagtttttg caactgcatg cacggaatta ccctggtggg
    23821 gttttctctg agcatgtttt cacccggatg aaggaaaaag atatattgcg aattgaaggg
    23881 ccgttgggat cattctattt gcgggaaccc cctagtgata aaaccccact aatttttctt
    23941 gcgagtggaa ccggtttcgc cccgattaaa agcattcttg agcatattct ctctacactt
    24001 cctttccaag agagtcatcg caagttattg ctttactggg gagtgcgcgt aaaagcagat
    24061 ctttacttaa atgacttggt cgaacagtgg gctaacacct acaaaaattt tagctatatt
    24121 ccggtattgt cggatgcttt acccactgat caatggaaag gtagaaccgg tctagtccat
    24181 caagccgtat tggaggacat tcccaatcta gcatcctatc aggtttatgc ctgcggttct
    24241 cctttaatgg tcaaggcagc ttatcaagat ttcactcaat tgagaaatct acccgccgcc
    24301 cagttctttt ctgatgcgtt tattccctct gataatcaaa tgccttagct tttatattgc
    24361 ctgcttctgt aaagtaaaac ctaaaccttt atgataatga tcagaagcta gctcacgttt
    24421 atccagtttc tcaaataatt gagcaagggc caaatgcgct gcacgacttg gttcaattga
    24481 taaacttgct tctaggtaat tttgtgcttt accccacaat tcacaataag tacacaactt
    24541 acctaatgtt agaagaagct cagcattatt aggattttta ctcaaccagt tctctgcacg
    24601 ttgaatttgc caactgacac tattaccaag gcattttgaa taaagtgtaa taagctcatt
    24661 atctaatttg ctgtctagac tttgctcaat gactctttgt gcagttgcca tatcattcaa
    24721 cgcgatataa ccttttgcag ctgtggcagc caagcgactg tccaattgct ccattgaatt
    24781 caaactatgc cagtatttat tgagaagatc taagttcgta ctatttttct tgatgctctc
    24841 ttgctgagca tggtgcttga gttgctcaat cgagccttta ccataaggat aacgatttgc
    24901 aagaatattg accaaatcca tgactgcatc ccagttttgg gccatttgct gcgccttcaa
    24961 ctccaactgc aatgccgtaa tactttgcaa gccacctaaa gaatataaag aatgcaacgc
    25021 atcaagcgct tctttatgac gaccttcctc tagtagcaac tcagcttggg ttatcagccg
    25081 aagcgtatgt tcaccgggcg caacttcttc agaagcagct aagaattcat cgcgtgccgt
    25141 gtaatcttct agcttatgcg ctgctcgtgc tgccaatgct gcgttaatac cattaatgat
    25201 cggagaactg ttcaatctaa gggctgaggc tgcagctttc tgagctctat catattttcc
    25261 ttcaaaaaat gcctttaagc caaccagggt tcggtcattg attcttttgc gtcgaaagta
    25321 aaaactaaac cctaaaattc gcgctaggat tttaagcaaa acatagaaaa taaagaacgc
    25381 aacaattaat aaaatgaaaa aattgttaat cgtcaactca accttaaaag gcggcacatt
    25441 aaataaaacc gagccactat tgaattgagc aacatataca atcccaactg ctgctgcaaa
    25501 aagaaataaa acccaaagca ctagtctcat tcatctcctc cctcatttgt aacttgaata
    25561 tagtgtgcca tttcgatggt tctgaaataa ttgttattat gattctttgt cttctaaacg
    25621 atagctatgc agcgaatcaa gtactttgag cacttcgaca actgattctg cttgtttatc
    25681 aaagtgacgg tccattgaag caacgacctc gccaagatta gattgaagat ctgcacgatc
    25741 ttcagatgat aaagctagct gtgctagcgt cagttttaat tgtatatttt ttcgtaactg
    25801 atcagcttga gctggcgaga taatttcaat gctcgaatca tcgattttct gaatactgat
    25861 cagtttttga agatctttgc aaatttcaga taagaacaaa tatagcttac tattttgcaa
    25921 agtttcggaa tgcggcaatg cgaaatccaa cttaacaaag tgacgatcca tcgcaagcgg
    25981 taaagtctcg atcacttgag aaatggatgc aagcttttca gcgattgctt gatcattctc
    26041 ggagagcgtc gcttttattg caccaagttg cttttccgca ttactcaatt cagtttcaat
    26101 atcagcgagt aactgacgcg gcttcttccc cgttaaatca actttatcta aataccctgc
    26161 aattgcgtgt ttcagatcga caagatggcc actcttcttt gcccagaacc agcccatcgc
    26221 tgcgagcaca attatcagaa caagaaaagc caacaaggcg ccctgctggg aagatcgatg
    26281 tttagtatgc tggattcttt ccatatttcc aataaaccat ataaattaac gattaaaata
    26341 actcaacaga cccgcaatca aaccatcatc accgctctca gtcaaattaa tatctttcaa
    26401 accaagatct ttcgctattt ctgcaatccg ctcatgcgcg gtgaaaaacg gtgtattttt
    26461 taacaattgt tggccaactt cccctaccat gtcaaataaa ttgtgtaatc cttcactact
    26521 ggttatcgtt actgcatgaa cagatccacg cgaccaatac gctagtaaag ccgacacatc
    26581 agtttttggt ttattacgct gatagcactc agcgtattcg acaatagcac tgcgattggt
    26641 taaggtttca cctaataatt ttcgcccttc attaccgcga aaaatcacga tacgtttttg
    26701 ctgaacctgc ttcagtatct cgagctccag caatgcttca ctatcaaacc gatgcgctgg
    26761 taagattact ttatcaatcc cataagaaga tagcttctgt gcgctcccct taccgaccac
    26821 agctactttt aggtgagaag gaaaaggggt atttttacaa attaagggta gtgccttatc
    26881 aaccgcgtta ggactgacaa aaattgcaaa atcaaattca tgcagacgat cgattatgct
    26941 gagtaacggt cgaacatcct caacatcggt tatctcgagt accggaaata ataaaggctc
    27001 accccccagt gatttaattc ggttaacaag ataggttgcc tggtgagccg gacgcgtgac
    27061 taaaattgac aatccagaaa gcgcaggcat aggaattaat catgcacagc ggctatgaga
    27121 ccacaagtgc agctagtatt ttatcagcac cttgtgccat caagttttgg gctagctgtt
    27181 cacccagcgt agccccagac tcaggtttcc catttaattc atcgctaacg attctttttc
    27241 catctggagt cgctacaaac ccacgcaaag tcaacacatt gtcggtaatt tgcgcaaacc
    27301 ctcccaatgg tacttggcaa cttccaccca acactctact catcgcgcgt tcagcttcta
    27361 cacattgcgc ggtttcccta tcgtgcaagg gttccatcaa gcgaactaaa tcggggcgat
    27421 tatcaagaca ttcaattcct agtgcaccct gaccaaccgc tgggaggctt tgttcaggtt
    27481 ctagtaacgc agtgattcgg taggataaat caagtcgttt caaaccggct gcagccaaga
    27541 taatcgcagc atattgacct tcatcaagct tacgcaatct tgtttgcaca ttaccacgca
    27601 ggggttgaat aattaattgt ggaaaccgtg cgcgtaattg ggattccctt ctcaagctcg
    27661 atgttccaac aacgctacca gcaggcagct ctgataggtc tttatattga ttcgatacaa
    27721 acgcatcacg cggatcctca cgttctgtga tagcagctaa gacaaaccct ggtggcaaat
    27781 tcatgggaac atctttcatg gaatgaaccg caatatctgc gcgaccatca gcaagcgctt
    27841 gctcgagttc tttgataaac aatcccttgc caccaatttt tgacaaagtt acatccagga
    27901 tttgatcacc ctgtgttgtc attccaagta tgctgatttc ggtttgtggg tataattcac
    27961 gtaaacgatt tttaataaaa ttagcttgcc acattgcaag aaggctctcg cgtgaagcaa
    28021 tcacaatttt agaaggaata gctgctgaat ttggcatggt actggtattg aaaaatgact
    28081 aagcttattt aatttacgct cattctagca tgatccgttt taatataaac tgcacgaaaa
    28141 tagaataagc agtatttcga acacaggcat aaagaggggg tacgagtgat agatgttgac
    28201 gccggattaa acgataactc tgatgaagac tcagaggagg ggaaaaacac gcccctgcgg
    28261 cacgacatcc gaatgttggg ccgcatgctc ggagatacac tccgtgagca cgaaggggaa
    28321 gcgaccttca atctcgttga gtcaatcagg caaaccgcta ttcgtttccg acgtgatgga
    28381 gaccctgtag cgggccaaga gctcgatgcc attctcaatc gcctaaaaaa taaagaaact
    28441 atcgcagtgg tacgggcatt tagctatttt tctatgctgt ccaatatcgc tgaagattta
    28501 catcataacc gccgccgtcg tattcatttg cgagcgggat ctcctcccca gtccggtagc
    28561 gtaacactcg cattagaacg agcgctgcag tctggcatta atggcaaagc attagaagat
    28621 ttttttgaca aagcggtagt atcaccagtc cttacagctc acccaaccga agtccagcgc
    28681 agaaatattc tagattgcca acttacgatt caacgcttat tgaccgaacg ggaccgcact
    28741 caattgacgc ccaacgaagc gcgccataac gaagaggcga ttcgcgccgc aatccagatt
    28801 ttatggcaaa cgcgaatgtt acgttcggtg cgactaacgg tacacaatga aattgaaaat
    28861 ggcctcatct attatagtta taccttcctg agccaagtcc cctacattta tgcaaaaatt
    28921 gaagacttgc ttgaacgtcg cttaggggac caagcccctc ccgtagcatc ttttctgaaa
    28981 ataggtagct ggataggagg ggatcgggat ggcaatccat ttgtaacaca ccaggtcatg
    29041 ctgcatgcgg cggaacgcca ttcagctttg gtttttgatt attatattaa tgaagtctat
    29101 cgcatcggcc aaacgatgag cttggccgag cgactgatta aagcaagcga tgaattagaa
    29161 caattagccg ctgcatcacc ggacattcct gctagccgca ttgatgaacc ttatcgaaaa
    29221 gcttttttag ggatctatgc acgtctgatt gccaccagtc aacgattggg gcacgcgatt
    29281 acgcctcgca atcaggtcaa agctggggaa tcctatgcgg atagtgcaga actcgttcac
    29341 gatctcgaca ttattatcca ttcgttaaaa aagaataaat cgcattggat tgcaagaggc
    29401 gcattacgcg atttgcgtcg cgcagcggat gtattcgggt ttcacttggc acccttagat
    29461 atgcgccagc acagtaagat ccatgagcaa gttatcgcag atctttttgc acaaagcgtg
    29521 cacaaaagag attatttaca gctcacgcca tcagaacgcc aggcgctact cttagatgaa
    29581 atcacgagcc ccaggacact ctattcgcct tattttaagt attcagctac aacacaaagc
    29641 gagctaagaa ttttaaaaat ggcagccgaa attcagcatc ggtttggtca tgcggcacta
    29701 cccaattaca ttatttccat gaccaccagc gttgccaata tcttggaagt agttttactc
    29761 ctcaaagaag ttggtttgct ccattctgga gagaatccaa gactcggttt aaatatcatc
    29821 cccttgtttg agacaattgg tgatctaagg ggttgtggcg caatgatgga tgaactcttt
    29881 tcagttcctt attaccgtaa attgctagaa tcacgcggta acgagcaaga agtcatgctg
    29941 ggctactctg acagcaataa agacggcggc ttccttacct ctaactggga aatctacaaa
    30001 gctgaaatca atttaactga agtctttgct aagcatcaaa tcgaattacg attattccat
    30061 ggtcggggag gcaccgttgg ccgaggcggg gggccaagct atcagggcat tatggctcaa
    30121 cctcctcata gtgttaatgg gcaaatccgt gtgacggaac aaggtgaagt tgtcgccagc
    30181 aaatataccg atcccgaagt tgggcggcgc aatctagaaa cactggtcgc tgcaacgata
    30241 gaagcgacat tattaagcca tgaaacgatc caggctgatg tgacgcgtta tcatcaagca
    30301 atggaaaccc tttcttcgca tgcgttttca gcttatcgaa atctcgtata tgaaacacca
    30361 ggatttaagc aatttttctt ggaatcaaca cccgttcgtg aaattgcagg ccttcatatc
    30421 ggaagccggc ccgcttctag aaagccatcg gatcgtattg aagatttacg tgccattcca
    30481 tgggttttta gctggagctt gaatcgaacc attattccag gttggtatgg atttggctct
    30541 gccgttgaaa aattcattca acaacatgat caagatgaaa ctgggttaaa actgctacag
    30601 gaaatgtacg ctaaatggcc attgatgcaa acattgctgt caaacatgga tatggtactt
    30661 gccaaaactg atctgggtat tgcatcgcgc tatgcggaat tagttaccga tgttagcttg
    30721 cgtgagcaaa tctttagtcg catccaagaa gaatggcaac gtagtgtcaa atggcttttc
    30781 caaatcacgg gtcatagcga attactcgaa gataacccca cgctcgctcg cagtatccaa
    30841 attaggacac cgtacatcga tccgctcaat cacctacaaa ttgaattgtt gcgtcgctat
    30901 cgtgcgggtg gtgatgttga agaacaagtc catcgctcca ttcaccttac catcaacggt
    30961 gttgcggcag ggcttaggaa tagcggttag tatcgattct ttgaaggaac tgaaccttgt
    31021 cagttccttc tacattccca gattgattga ctatcttctt agaaaggata agtagataca
    31081 aacaccagat catccgccac gatcggcgtc ccgattacat taatcaccga atcgatatca
    31141 gcacttccat tgccttcaat atcgacttcg atacgctgtg ttcccaaccc tagatcataa
    31201 gttagccgta cttcactcac accactggca gtaaatggag cattgcctag aaataccacg
    31261 ctattcggtg accattctgt cgattgcaaa attattgtat cttctaaacc attaaaatct
    31321 gtaatgagat ctggcgcgat cgggtttgaa tcacttccag tcccgctact gcttatagta
    31381 aatataaacg tatcgctatc actcccaccg gttaggctat ctgcaccgcg gttaggcctg
    31441 agcaaatccg cccccgaacc accatccaag acatcgtttc ccgttccacc acgaatttta
    31501 tcgtcaccat taccgccata aagactatct cctaggagat caccggcacc actgaatatt
    31561 gcatcattac catcgccacc attaatgata tcgctaccca tcccagcatt aagccaatta
    31621 tctaggttag agccggtaat cgtatcattg aaatcgctac cctggacttg ttctacatta
    31681 agaatgaggt caatacctgc tccaccggta ttttgcgacg taatcaaact caaatcgact
    31741 gtcacaccac tgatcaaacc ctggcgctga taattaatcg tatcgatacc atctccacca
    31801 tcaatactat cgtttccaat aatccccgta tctggaacca agagatcatt tcccaaacca
    31861 ccatagagtt tgtcattccc agcgccacca tcaatctgat c
//
LOCUS       DAOQLJ010000007        31533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_451_length_31533_cov_6.913527, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000007 DAOQLJ010000000
VERSION     DAOQLJ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 31533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31533
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_451_length_31533_cov_6.913527"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1795)
                     /locus_tag="PK522_01140"
     CDS             complement(<1..1795)
                     /locus_tag="PK522_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNC40368.1"
                     /translation="MIQLIQVLMFVMALVITTNETFASTESAQFSINLFQDGLPISGA
                     EVSVISDQFGRPDARFIDSSATSYAWLAKDGSPITTSANGSIAGKLPPGIYQIRIRTQ
                     RDQFFSFELPLRPTEDVQILVTFYDDPEKTLLNIESSEAGVLAGTEKAPITAESSGEG
                     TIVAQVVSAETQKPIKDVQLFISGLNQRLRTDDQGQVQINIPIGAYSVSLLHSAYNSQ
                     TRDSVEINKDQLTELKFSLTPAGVELAEYVVLEPHLAGSLTAVIEEQKSSAEVATIMS
                     AEQFSRSGDGDAASALRRASGLTLVGGQFIFIRGLGERFSSTLVNGAAIPSPDPTRRV
                     VPLDIFPTSVLESVLVQKTYSPDRPAEFAGGTIELRTRGIPDQFFFNFNGSIGVIDNS
                     TFAKGLTYKGGDLSFLGYDDGTRALPNSLADIVKDGGKLEPKTPFNPNGQTPAELEQI
                     GEGLATDWDIKRKDNPPLGNMQAAMGDVFNLGNFRAGYIASAGWNQEIRNQNEVSKLF
                     VATQRNDDQLLKVQDFDVKRTLREVQLNGYFGADVEYKDKHRLFTKTMFLRQSVDEGR
                     ISEGFTDNNPTEIRRTRLRLFSNQLLMQQVGG"
     gene            complement(2087..3253)
                     /locus_tag="PK522_01145"
     CDS             complement(2087..3253)
                     /locus_tag="PK522_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC40369.1"
                     /translation="MMLIATKRAIIVAIVLISGYFQPALAKEEKPQSDDFIELAAIML
                     KDGHYDRALLALQSVNLDDEKTDLARFYTLQGLAYMNLNDFDASKDSFQTAIKHGQKD
                     PLIYAYLAQIHFSLKDYQGVLNVIDKTGEHKSKNPALTSIQAQSYWHLQKTVQAIETL
                     NKGHQLFPSDYRFLKLKVFYFVQLQLYQEAANLGREYLARSKAQAEEYVAIGNALRLS
                     REFQEALKIMEIARLKFPDNEVVAKLLAHIYLDQGMLNAAAHILEQAARINSDLLSEA
                     AEVYRRAGRLHKALMLNSGINDQKVKLKQRLSILLALKQYDQAASMEASLYRVGLFDD
                     QSIRYALAYAQFSSGRFQQASKHLDFLTEPELFKKGIELRRLMEVCKEEPWKCA"
     gene            complement(3275..3913)
                     /locus_tag="PK522_01150"
     CDS             complement(3275..3913)
                     /locus_tag="PK522_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNC40370.1"
                     /translation="MIKKPMNKHIAATISMLMGPILVFGMVILMNKFADSLDKTPAQE
                     VTEISMVKQPPPEPKQEIKKVEPKKQPVRSNTPPPFKGLGSSLSGIDLGLLGFDNGGM
                     TGVDESLLGKTGNAVMTEDLVDVPPKPKSRSSFRYPPAAKKNGIKGYVVLSVLVDTDG
                     SVKQVQVLESNPSGVFDDAALQGVRSWQFEPAKYKGDSVRVWAKQKIRFDLS"
     gene            complement(3938..4342)
                     /locus_tag="PK522_01155"
     CDS             complement(3938..4342)
                     /locus_tag="PK522_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNC40371.1"
                     /translation="MRYSEPPETETAIDMAPLIDIVFILLIFFMVTTTFVKDMQLELE
                     RPKASSAVSSSSKAIRLFIDRNGDTYMDGEPVRIWLIQSKLRDLLSTSTNKVVLVVTD
                     EGVPAGKLVEVVDQARLAGAQSVGVATRKEAG"
     gene            complement(4373..4966)
                     /locus_tag="PK522_01160"
     CDS             complement(4373..4966)
                     /locus_tag="PK522_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNC40372.1"
                     /translation="MTLSEIVSLVEELFQAGGLVMPPLFCCVLLLWYGLGYRFWVLKT
                     PQKMGVRDLLSFYREGKFRDAQSIVEQAIQKGVALKKKQSQYLSYQLDAAFYEFEREI
                     ARFSVLVKIIVIISPLLGLLGTVIGMVETFDSLASMALFTQTGGIAGGISQALFTTQM
                     GLAVAIPGLLAHSFLSRKQQQIEKDLLQVKKLLCRLT"
     gene            complement(4988..6523)
                     /locus_tag="PK522_01165"
     CDS             complement(4988..6523)
                     /locus_tag="PK522_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNC40373.1"
                     /translation="MKPFLLVGLFCYLISTAWAQETTPEVSQATETITTEPASDKKTN
                     TNNPGKAASAKKNTTSDSKPVNLETAYKREYAFLDAQKRELKAQLDKYKASANSKEQE
                     LIRKINALERESVSRSAKIDQFNSQIVEAERTQAAVSERNDALEITYAQSELTLKQYG
                     IEMPPPIKEGKENDLEKTTFLLQQGLTLIQKLGEIQSKAGKFFLESGKQAEGSIIQLG
                     NIAAFGVSADGGGALVPAGNGEFKVWKEPAAEIATALNNNEQPDYLKLFLFESRTTAI
                     DETPDKTVLSIIESGGLIGWVIVGLGALALLLAFIRAFLLRKNSTDNKQLADRIIALI
                     AEGRLAEAKKICESGGSAISRVLASTLRHLKDDREHMEDIVHEAILQEAGPLDRFGSA
                     IMVIASVAPLLGLLGTVTGMITTFDVITEFGTGDPKLLSGGISIALVTTELGLIVAIP
                     TLMLGSLLNAWARNIRRDIEHSALRATNAFLGGKSGNSVATDPGAGNDPETQSFRLQP
                     QPI"
     gene            complement(6520..7290)
                     /locus_tag="PK522_01170"
     CDS             complement(6520..7290)
                     /locus_tag="PK522_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HNC40374.1"
                     /translation="MKKCFEGLLTLIGLSIITLYAPTVTADNLENLAKSLAKIRGEVE
                     ELQSQLDLEKENHNSRIASLSSQLADLSVEERRQKISIEKLQQSIEKLTAESKQAEHS
                     GDNLKPSLILFLAEYKRFIQAGLPFKIEDRLKAIQELEDNLNNKLIDPKKAVNHAWSL
                     LEDEVRLSKENGIYQQTIELNGEKILADIAKLGTIFLYFQTRDGRFGMAQHTEGSSWK
                     FVSMDTKKDKTQISQLFDALKKQIRQGYFELPNPLKKL"
     gene            complement(7924..8757)
                     /locus_tag="PK522_01175"
     CDS             complement(7924..8757)
                     /locus_tag="PK522_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNC40375.1"
                     /translation="MTADNNNPRLAVLIDADNTFKVIDLIDELFEEISKFGDASVRRI
                     YGDWTQNQLSRWKEILPKHAIQPIQQYANTKGKNATDSALIIDAMDLLYTAPLEGFCI
                     VSSDSDFTRLAIRFRESGKLVYGFGEEKTPESLQAACNEFKYFEILSQNKHIGIGDSV
                     ATQAEDIPNKSKTSLKKKTTKSVSFSSEVTTIPGTKKNSRELAMDTKLVSLIRSAIEA
                     ISDEDGFAYMGEVKKHIVKNFSAFDSRNYGYAKFSDLIKAIGLFEVDTQNQHIKDKRK
                     K"
     gene            complement(9007..11124)
                     /locus_tag="PK522_01180"
     CDS             complement(9007..11124)
                     /locus_tag="PK522_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HNC40376.1"
                     /translation="MSSKLHPDSKLKINDITCNESSNPSMMEIIEQRKISRREFLKSS
                     VTATAGITTMTLLGTAAMESMASFAEDISKHGLLLGFNPVSPNLQPMIDGITVPEGYT
                     ARVLISWGDSINDKPDWDTVSAMDEETQLHCYGAHTDGMHFFPWPGQAGNQRGLLVSN
                     SEYCDPPLVNNITPASDYAKVPITLAMVRTQQAAHGVNIIMIEKKRKKWSLSRNWYFN
                     QRITGNTPCVISGPAAGHELMKTAADATGKNVLGTLNNCSHGYTPWGTYLTCEENWNG
                     YFSNESGDVIGELDLDRKLNILKGQSRYGVVKGGFGYRWHEVDERFRADLHPHEPHRF
                     GWVVEIDPRSPWDKPIKRTALGRMKHEGAQVVVDRQKHVAFYMGDDERNEYIYKFVCA
                     KKWNGNPQVTDPDMLDKGTLYVARFNADGSGDWMPLIWGQNGLTPENGFGSQAEVLIK
                     TRQAADRLGATMMDRPEWGAAHPETGEVYMTLTNNSRRGNTPASSNQIDGTSNAGSAN
                     PSVDAANPRPDNDYGHIIRWREDDANVTATHFSWDIFVQCGDRDSAKALGASYNPAGS
                     DGYKGNINGDDYGAPDGLWFDKDGRLWIQTDQQGDAKGDWINIGGNVMMCANPQTGET
                     RRFLTSPPNCEVTGVVTTPDGRSMFVGIQHPGEDWKINFTDNSSWPDNGYNGPTTFTG
                     AVVSKPRSSVIVITKDDGGVIGT"
     gene            11770..12285
                     /locus_tag="PK522_01185"
     CDS             11770..12285
                     /locus_tag="PK522_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40377.1"
                     /translation="MKKLSLILVITTIMLVSSFNLMAACAPWVQTRVTQPDYAVLDAA
                     LTSAIQTRLKNKLNATKDEPTYSALRIEAEGLAATIANGRVVITMPDGTVVVDTSKGA
                     NNTYDNFKAKTINENHNSRIAILHSQLYDCGLGLETKLSTTDNTKENYLARRLGAYLN
                     SAGTVRLSQKL"
     gene            12662..13978
                     /locus_tag="PK522_01190"
     CDS             12662..13978
                     /locus_tag="PK522_01190"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0008889 - glycerophosphodiester
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNC40378.1"
                     /translation="MDFERTMAIIASCMLFSITFSMVTKNSLAASDHGQKDIGQVKVS
                     EIKKTNIQLGPRPFFLVDDMSEGALKNKLKSCANKVSRKTEFSIGHRGAALQFPEHTK
                     ESYLAAARMGAGIIECDVTFTQDRELVCRHSQCDLHTTTNILAVPELAAKCSVPFSPA
                     EIDPITGEIIKPASAQCCTSDITLAEFKMLNGKMDASNPAATTPEAYLGGTPAWRTDL
                     YASKGTLLTHRESIELFKQLGVKMTPELKAPSVPMPFQGDFTQQDYAQKLIDEYKLLG
                     IKPEKVFPQSFSLSDVLYWIQNESEFGAQAVYLDGRYDDPTFDPQNSASWNPSMSDLV
                     SQGVKIIAPPMWVLVTVENNRIVPSVYAKAAKEAGLDIITWTIERSPPLIANGDWYYQ
                     TIDSVVNKDGDQYVLLDVLTKQVGILGIFSDWPATVTYYANCMDIK"
     gene            complement(14053..15237)
                     /locus_tag="PK522_01195"
     CDS             complement(14053..15237)
                     /locus_tag="PK522_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40379.1"
                     /translation="MKKHVNEQKHLVGSLLRSGLPITWFRCKGNATNDCLIEFALDHN
                     RSKSILYRRLGYRFLLRRYLPQWVRISILQQPISTQYEMHSALVYEPPGDSIHTIIAF
                     PGLSVHGYRDHRMDAVARAFSYLGFRVIIPCVSDIEQLIIHPSTISKFAALIEAIATH
                     SELNPNHRQLGIFSASYSGGVALLAASHPQISSSIKAMCLIGTFADFRNVIRFVLDQE
                     EIDEYARFILLRNLLSQSSYRNPEVTQLFDIAVADNGFKKKNPLLPYSLQNTSKEATH
                     FFCQLLEDAFFRHQVAQSALEEIDRREHWLNRFDLDKQLTHIDFSISLIHGYHDQVIP
                     SHESIFLHKRLAQLGKKSHLILTSLLDHGDVVLNKNFFVETDKLAQGLGFFVHELCES
                     TS"
     gene            complement(15155..16165)
                     /locus_tag="PK522_01200"
     CDS             complement(15155..16165)
                     /locus_tag="PK522_01200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNC40380.1"
                     /translation="MKIALITDAWQPQVNGVVTTLVELVAELEKFDHQFMIIHPGLFN
                     TRPCPGYAGIDLAIRPRKHLQKLLLQYNPDAIHIVTEGPLGWAARSICLKEGWFFTSA
                     FHTKFPEIFKASLGVPVFVGYTVLKHFHQPSSGVMVPTQSVLEGLQKRGFQNLKSWSH
                     GVDTELFRFHEKPAEFPALGRLPRPISLFVGRISYEKNIQAFLDLDIPGSKVVSGVGP
                     LESDLKRRYPNVLWLGILPRNELVQLYASADVFVFPSAADTFGLVLLESMATGTPVAC
                     FPVDGPLEVIGDSGAGAMLPDLKEAVLSAFEIPREKARERAETFSWESAAKRFADYLV
                     PV"
     gene            complement(16162..17103)
                     /locus_tag="PK522_01205"
     CDS             complement(16162..17103)
                     /locus_tag="PK522_01205"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNC40381.1"
                     /translation="MRDHYAYLHNQFNKPTDNWQSNSELDDSPEENYKSSVRAIFISD
                     LHLGTTGCQASALLDFLKTYSSEQLYLVGDIIDGWQLRRRWYWPQAHNDVIQKLLRKV
                     RKGCKVIYVPGNHDEFAREFLGHSFGGIEIVEEAVHETVDGRKFWVIHGDYFDGVIQC
                     AKWLAYVGDNLYEFTLKLNRYLTSLRARLGLPYWSLSQYLKYKVKTAVNYVLSFEQAV
                     AAEASRRGLNGVICGHIHHAEIRDINGVTYCNDGDWVESLTALVEHFDGSIELIHWPQ
                     HALQLTNTVEIPLTEGCLPIGKPVVTNKIKDNEKVLA"
     gene            complement(17106..17954)
                     /locus_tag="PK522_01210"
     CDS             complement(17106..17954)
                     /locus_tag="PK522_01210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HNC40382.1"
                     /translation="MSESIMATAEMLQDSFFRDSTSSIPEVGQCATTKLESQTNIVTT
                     WARHLSEVREAQKLRYQVFATEMGASLPNILSGYDIDIFDDYCEHLLVRDRKTWQVIG
                     TYRVLTPAQAKLAGKLYSDSEFDLRALQPLREHLAEIGRSCVHKDYRQGNVILALWKA
                     LVQFMTQNKLDDIVGCVSIPMNMQVKDGQIGMGYAAASIWRQMKESHLASQELQVIPR
                     LPLPVEQLDQTLDIDPPALVKGYLRVGAKVLGAPAWDPDFNTADLPMLMRMRNLPERY
                     RKHFLG"
     gene            complement(18300..19898)
                     /locus_tag="PK522_01215"
     CDS             complement(18300..19898)
                     /locus_tag="PK522_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40383.1"
                     /translation="MTRFSKLNIRLTAITAAVMSAFAINVQAATATYNPSDNTVDLPI
                     VEVLDGATSNFFSAKLQLVNGNELVLVDAQPIAPAKNVQRNVYDSATTAVHIASVDAG
                     GAEFYAKLKLIPGSNPLRFSLEQLVNNQFESCPDFATPGPIANSCILSGEIRNRNITL
                     TKNTQWILSGGVFIGGDKTENATITINPGTKIVGQQGADYLWIRRGSKILAEGTPDNP
                     IVFSGPNEQAAGEWGGVVLNGFARINGCNEGTAVCEVANEAITTEFSGGNNDADSSGI
                     MKYVQILFAGFAVRPDEELNGLTLNAVGSGTVLQFIQVHRGLDDGIELFGGTANLKHI
                     VLSGIEDDSLDWGLGWRGKAQFVLIQQSASDGDHGIEADNNPNNPDIAPRSKPVLSNM
                     TLIGSPVSQASGALLRRGTGVNIWNSIFTGFQNCLTLDGTATSALVGQASELSINNSF
                     VNGCQNSFAESGGATASTADWFNSQTGNKAQDPLLNGFLPNAGSPVLQAGATLSDSYF
                     KPVSYAGAFRDANDNWTQEWTFPF"
     gene            20223..22034
                     /locus_tag="PK522_01220"
     CDS             20223..22034
                     /locus_tag="PK522_01220"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC40384.1"
                     /translation="MEASQPDTKNPIWIARETLKHLATQRIAPTPDQYQAIYYQIAGI
                     NPPKHDVSDSLANNLLAILRTLSLPQAANRYVVDINLAVGNENWPTVAQLVGQLIQLQ
                     ASPNQQLERPWEELLLMLWDARGKASDDHTRRDELAYTVALFAGQPEKLNHELNTLIT
                     HWLNKPHEIEVFDNENSPVEIMPMDSLAQENCSQTQYPWQQWQALVVNTLRDGLIPRL
                     TSHQHWIDEIEFIIQAIEAIEQPENIDWIQKRLRQCWIHMALRIDQEQRLIDGLLSVL
                     ALFVEHLSLPSDLDPWVTHQLDNIRRNLAKPLTLHRIYDAESILKELVYQQGLLQHTL
                     TEAQQTLKAMISVFLERLGVVTEATGEFQHKIDHYTERVHQTSQAVDFKSLLEELMED
                     TRHMQLDMLRARDELVKTQQQADAAQQRVNELRQQLRQISEHNRDDRLTGALSHRALV
                     ETFSAEQSHMSQSGKPLCLALLDVDNLSELNAQLGRQAGDDTLTGLVQVIKTVIREGD
                     SVIRYGSEEFLILLPNTPLSTATDLLRRLQRELTKRFFMNNNKRLLITFSAGVTRCLS
                     QESREQALDRADYAVFLARKQGKNQVAVVEAPEIPVN"
     gene            22551..23153
                     /locus_tag="PK522_01225"
     CDS             22551..23153
                     /locus_tag="PK522_01225"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNC40385.1"
                     /translation="MFTGIIEAVGIIDQISQNPKGVSLQIDAGKLDLSDVKIGDSIAV
                     NGICLTVTKQGECYFAVDVSHETLRCTKGLKNLGSQVNLEKAMRLSDRLGGHLVSGHV
                     DGVGEVIQFEPEGENYRLSIQAPDALLKYIVVKGSVTINGVSLTVNRIEGKVFDINLI
                     PHTLAETNLKLLKVGEAVNLETDLLARYVERLMTQSINQI"
     gene            23162..24274
                     /gene="ribBA"
                     /locus_tag="PK522_01230"
     CDS             23162..24274
                     /gene="ribBA"
                     /locus_tag="PK522_01230"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011113009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNC40386.1"
                     /translation="MTISSIETIVSDFKAGKMVILVDEEDRENEGDLVLAAEFVTPEA
                     INFMAKFGRGLICLTLTEERCHQLKLSPMVAANHSSHGTNFTVSIEAASGVTTGISAA
                     DRARTIQVAVKKEAKPEDIVQPGHIFPLKAQKGGVLVRAGHTEAGCDLAQMAGLMPAA
                     VICEILKDNGEMARLPDLVEFANTHQLKIGTIADLIHHRSLTESLVSRIAERTIQTVF
                     GEFDLKAYHDKTANITHLALVKGQLQSEKEILVRVHEPLSVIDVLDIADCSHSWSIND
                     AMRVIAEAGSGVIVMLHRKGDAGILTERIMATKSWYPDQIKTDLRDHGIGAQILKDLG
                     VRKMRLLATPRKMPSMTGFGLEVTGYLESSNKQTAT"
     gene            24315..24794
                     /gene="ribH"
                     /locus_tag="PK522_01235"
     CDS             24315..24794
                     /gene="ribH"
                     /locus_tag="PK522_01235"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNC40387.1"
                     /translation="MGYYDNIPEIETNLDGSELRIGIVMSRFNIEIGEGLLGACTAEL
                     VQLGVQEANIQLVTVPGALEAPVTLQKMALSDQFDALIALGAVIRGETYHFEVVANQS
                     AGGLMKAQIETGIPIINAILTTDTEDQASSRMSQKGKDAARAAIEMANLFDKLVEFE"
     gene            24806..25300
                     /gene="nusB"
                     /locus_tag="PK522_01240"
     CDS             24806..25300
                     /gene="nusB"
                     /locus_tag="PK522_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNC40388.1"
                     /translation="MKEAPAKSGPKPAPKFKNRRRSARELALQGIYQWRLTASEAGFI
                     EAQLRSASFFARVDAVFFSDLLQGVIKHADELEQKIAPCLDRTFQELSPIELSILLLS
                     TYELIYHPNIPYRPIINESIELAKTFGGTDGHKYINGVMDKLATQLRGTEVALHAKKT
                     AKFH"
     gene            25401..26372
                     /gene="thiL"
                     /locus_tag="PK522_01245"
     CDS             25401..26372
                     /gene="thiL"
                     /locus_tag="PK522_01245"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNC40389.1"
                     /translation="MESEFDIIRRYFTRPTKKALLGIGDDAALIAYNATTDLAISTDT
                     LVVGHHFFPDTDPSQLGHKALAVNLSDMAAMGAVPRWLTLAITLPRDAPQTNSAWLQA
                     FAHGFFALADRYQVELIGGNTTNGPLNICVQIIGEIPRNKALKRSNAQLNDDIWVSGQ
                     LGNAALALAHLKQQLVLTSEALDVCLPALHTPTARVALGQQLIDLAHSAIDISDGLLA
                     DLGHILSSSNLAAVIQLETIPHSWVWNRYAAHPQALEWLLSGGDDYELCFTAPETERK
                     KIHTLSKELATPLTCIGKILQGEGLSIVNSTGTVIEHTKKGYDHFLS"
     gene            26353..26898
                     /locus_tag="PK522_01250"
     CDS             26353..26898
                     /locus_tag="PK522_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNC40390.1"
                     /translation="MTTSFPEPYRSNFKLPPLKPDIAFVYSRLAHFLAFAAGAGLSKY
                     APGTIGTLVAFPIYWLLQLLFDPIALLLVIDIFFIIGIWACGITGKALHAPDHSGMVW
                     DEIVAFLLVLYFIPDHWLWQISAFILFRFFDIVKPPPIKYYEKKFHGGVGVMFDDLLA
                     AFFTLLCLAGWKSIVLFESYF"
     gene            26908..27423
                     /locus_tag="PK522_01255"
     CDS             26908..27423
                     /locus_tag="PK522_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HNC40391.1"
                     /translation="MIVDDSVLSAYAQQVGVLLTSKHLRLVTAESCTGGWIGQSITAI
                     AGSSGWYECGFITYSNQAKHRMLGVQATTLLKYGAVSEQTAREMVTGALKMSTAQVAI
                     SVTGVAGPSGGSKEKPVGMVCFAWEFKQSAIHSTTHFFSGDRESVRRQAVAAALLGII
                     ELLEGISPSLV"
     gene            complement(27412..28026)
                     /locus_tag="PK522_01260"
     CDS             complement(27412..28026)
                     /locus_tag="PK522_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HNC40392.1"
                     /translation="MEINQKTDNKRISAVARFLFLSRWLQLPLYLGLVLAQCVYVFHF
                     WVELTDLVGAIMGNKESLKHILHIVTPEEAEAPLKITETAIMLVVLGLIDVVMISNLL
                     IMVIIGGYETFVSRMNLEGHPDQPEWLSHVNASVLKIKLATAIIGISSIHLLKTFINA
                     DAYDEKTLIAQTSIHITFLISAVAIAYTDRLMSQTLSHGEASNK"
     gene            complement(28148..29035)
                     /gene="sucD"
                     /locus_tag="PK522_01265"
     CDS             complement(28148..29035)
                     /gene="sucD"
                     /locus_tag="PK522_01265"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNC40393.1"
                     /translation="MAILINENTRIIVQGFTGRIGTFHAQEMIDYGSKVVGGVTPGKG
                     GQTHLGLPVFNTVKEAVEKVGAEASIVFVPPAFAADSIMEAADAGIKYCVSITDGIPT
                     QDMMTVKSFLRRFLKEQRMMMTGPNCAGTISPGRAMLGIMPGHIYMRGNVGIVGRSGT
                     LGYEAADQMRRLGIGITTSVGIGGDPIIGSSHRDVLEKLEQDSETKVALMIGEIGGPQ
                     EVEAGIFAKENMTKPLVAYIAGLTAPTGRRMGHAGAIISSAGESAAEKVERLKELGVT
                     ICPTPSLMGQTVAEVLAKL"
     gene            complement(29048..30229)
                     /locus_tag="PK522_01270"
     CDS             complement(29048..30229)
                     /locus_tag="PK522_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate--CoA ligase subunit beta"
                     /protein_id="HNC40394.1"
                     /translation="MDIHEYQAKEILAQYGVKIAEGGLAHTVEEAVQRSREIDGNVWV
                     VKAQIHSGARGKAGGIKVCKTHPEVEAAAGSLLGKKLVTHQTGAAGKLCSKLYIEAGT
                     DIARELYLSFLIDRSHERIIMVGSAQGGMEIERLAVTDPDAIIKIHIEPAVGLQDFQA
                     RTMAFALGLGEDLLNHAVKTIKGCYRAMRDLDANILEINPLVVTKSNELMALDAKMSF
                     DENALFRRQKIAELRDKTQIDPREVAAAEAGLSYVGLDGDIGCMINGAGLAMATMDMI
                     KLAGGEPANFLDVGGGASAERTEKAFRLVLADQGVKAMLVNIFAGINRCDWIAEGVVQ
                     AVKNIDMKIPLVVRLSGTNVEEGCRIIDESGLPIITAETLADAAEKVVKARNEVVAKQ
                     S"
     gene            complement(30278..31228)
                     /locus_tag="PK522_01275"
     CDS             complement(30278..31228)
                     /locus_tag="PK522_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HNC40395.1"
                     /translation="MSHTLYETKIQRVQRCELAVPGSRPEMFEKALKSGVDFIFLDLE
                     DAVAPDDKIQARKNIIQAINDLDWKGHGITLSVRINGLDTQYMVRDVVDLVEQAGHKI
                     DTILIPKVGVYGDVYMVEAMLNQLEMQQRLNNRIGIEALIETALGMANVEDIARNGAS
                     GRLEALHFGVADYAASNRARTTNIGGLNPDYPGDQWHAAISRMTVACRAYGLRPIDGP
                     FGDIQDPDGYKQAARRAAALGCEGKWAIHPTQIALANEVFTPLSAEVEKAKRILAALK
                     EAAAQGKGAASLDGRLIDAASERMANNIVKAAEAIAAKNK"
BASE COUNT         9052 a         7211 c         6328 g         8942 t
ORIGIN      
        1 agccacccac ttgctgcatc aataattgat tggaaaatag ccgtagcctc gttcttcgta
       61 tttcagtcgg attgttatca gtgaaccctt ctgaaattct tccttcatca acggactgtc
      121 tcaagaacat agttttagta aataatcgat gcttatcctt atactcgaca tccgcaccaa
      181 aataaccatt gagctgaact tcgcgcaatg ttcttttaac atcaaagtct tgtactttga
      241 gtaattggtc atcgtttcgc tgcgtcgcta caaacagttt actcacttca ttttgattgc
      301 gaatttcttg gttccaccct gccgaagcaa tataacctgc cctaaaatta cctaagttaa
      361 atacatcccc catcgccgcc tgcatattac cgagcggagg attatccttg cgtttgatat
      421 cccaatccgt tgcaagccct tcgcctattt gctctaattc agccggcgtt tgcccattcg
      481 gattgaaggg cgttttaggc tccaatttac cgccatcttt gacaatatct gcgagggagt
      541 tcggcaatgc acgcgttcca tcatcatagc ctagaaaact cagatctccc cctttgtagg
      601 ttaatccttt tgcgaaggtc gaattatcga taacaccaat cgatccatta aaattgaaga
      661 aaaattggtc cgggatacct cgcgtgcgga gctcaatggt acctcccgca aattcagcgg
      721 gtcgatctgg cgaataagtt ttttgaacta aaacactttc taatacgctc gtaggaaaaa
      781 tatccagtgg caccacgcgt cgtgtgggat cagggctcgg aatcgccgcg ccattaacaa
      841 gcgtcgaaga aaaacgttct cccagcccac gaataaagat aaattgaccg cccaccaagg
      901 ttaacccaga tgctcttcgg agcgctgaag ccgcatcgcc atcaccactc cggctgaact
      961 gttcagcact catgatcgtt gcaacttcag cggatgactt ttgttcttca atcacagcag
     1021 tcaaactccc ggcaagatgc ggttccaaca ccacatattc tgcaagctcc actccagccg
     1081 gtgtcaggct aaattttaat tcggttagct gatccttatt gatttctaca ctatcacggg
     1141 tttggctgtt ataagcggaa tgtaataatg agacgctata cgcaccaatc ggaatattga
     1201 tttgcacttg gccttgatca tcggttctga gcctctgatt caaaccacta ataaataatt
     1261 gcacatcctt gatgggtttt tgtgtctcag ctgaaacgac ttgtgcaacg atcgtaccct
     1321 caccagatga ttcagcagta atcggtgcct tctcagtgcc tgcgagaacg cccgcctctg
     1381 agctttcgat attcaataat gttttttcag gatcatcata aaacgtgact aaaatttgta
     1441 catcttctgt tggacgtaag ggcaattcaa aagagaaaaa ttgatctctt tgagtacgaa
     1501 ttctgatttg atagattcct ggtggtaact tacccgcaat actcccattg gcacttgtcg
     1561 taatcgggga accgtcttta gcaagccatg cataggatgt tgccgaacta tcaataaaac
     1621 gagcatcagg acgaccaaat tgatcgctta tcactgatac ctcagcacct gagattggta
     1681 aaccatcttg gaataaattt atcgaaaatt gtgcggattc ggtacttgca aatgtttcat
     1741 ttgttgttat aaccaaagcc attacaaaca ttaaaacctg aataagctgg atcatcttca
     1801 atcgttattc tttttattaa attactgcga gtacagtggt ctattagggt taaaagccgg
     1861 aatttatcta tgcaaaagga gatttagctg atgcgagagt agacagggca aatcgcactg
     1921 acctacttaa agcaagccaa tactactgat ggattgttaa attaatgtga cagaaataag
     1981 aatgctggct tagcagcgtg gtggagatga atgaccgcga ttacggtgtc aggtataaac
     2041 cgattctttg ccaatacacg attgcgaagc taagtaacta cctaacttaa gcgcatttcc
     2101 aaggctcttc tttacagact tccatcagac gacgtaattc aatgcctttt ttgaataact
     2161 cgggttctgt taagaaatcg aggtgcttgc tggcttgctg aaatcgtccg ctggaaaact
     2221 gcgcataggc aagcgcataa cgaatactct gatcatcaaa caagccgaca cgatataaac
     2281 tcgcttccat actggctgcc tgatcatact gtttaagggc gagcagaatc gataatcgtt
     2341 gcttgagttt aactttttga tcattgattc ctgaattgag catcaaagcc ttatgcaaac
     2401 gtcccgcacg acggtaaact tctgcagcct cagacaataa atctgaattg atacgtgccg
     2461 cctgctcaag gatatgtgct gctgcattca gcatgccttg atccagataa atatgtgcaa
     2521 gcaattttgc gactacctcg ttatctggaa atttcagccg tgctatttcc atgattttaa
     2581 gggcctcctg aaattctcga ctgagcctta atgcattacc aatcgccaca tattcttctg
     2641 cttgggcttt cgagcgagca agatattctc ggcctagatt agcagcttcc tgatacaact
     2701 gaagctgcac gaaataaaaa actttcaatt tcaaaaaacg ataatcactt ggaaaaagct
     2761 gatggccttt attcagtgtt tcgatggctt gcacagtttt ttgtaaatgc caataggact
     2821 gtgcctggat cgatgtcaac gcggggttct tacttttatg ttcacctgtt ttatcaatga
     2881 cgttgaggac accttgataa tccttaagtg aaaaatgaat ttgggctaga taagcataaa
     2941 taagtggatc tttctgccca tgcttgatcg cagtctgaaa actatctttg gatgcgtcaa
     3001 aatcattgag attcatgtag gccaaaccct gcaaggtata aaagcgcgct aagtcggttt
     3061 tttcgtcatc tagattgacg ctttgcagtg ctagtaatgc gcgatcatag tggccatcct
     3121 taagcatgat agctgctaac tcgataaaat catcagattg tggtttttct tccttcgcca
     3181 aggctggctg gaaataacca ctaatcagaa cgatcgcaac gattatggct cgctttgtag
     3241 ctatgagcat catcaaacac ctagttttct attcttaaga taaatcaaag cgaattttct
     3301 gcttagccca aaccctaacg gaatcccctt tatacttggc gggttcaaat tgccacgacc
     3361 gaaccccttg caaagccgca tcgtcgaaaa caccgcttgg attagactcc agcacttgta
     3421 cttgtttgac cgacccatcg gtatctacta aaacagaaag aacaacataa ccttttatcc
     3481 cattcttttt tgctgcagga ggatagcgaa aagaactacg tgatttaggt tttggaggaa
     3541 catcgacaag atcttctgtc ataacggcat taccggtttt accaagcaaa ctctcatcaa
     3601 cgccagtcat accgccatta tcgaaaccca ataaacccag atcgattcct gacaaagaag
     3661 aacctagacc cttaaaggga ggaggggtat tagaacgaac cggttgtttt ttaggttcca
     3721 ctttcttgat ctcttgcttt ggctccggtg gaggctgctt taccatactg atctcggtaa
     3781 cttcttgcgc aggcgtttta tctaatgaat ctgcaaattt attcatgaga atcaccatac
     3841 cgaagactaa aattggcccc atcagcatcg atatcgtcgc tgcaatatgc ttattcattg
     3901 gtttttttat catgcaaata ccccattcat tttttattta tccagcttct ttgcgtgtag
     3961 ccacaccgac actctgagcc ccagccaagc gtgcttgatc aacaacttca accaacttac
     4021 cggctggaac accttcatcc gtaacgacta gcaccacttt atttgttgaa gtgctcaata
     4081 aatctctgag tttgctctga ataagccaga ttcttactgg ctcaccatcc atataagtat
     4141 cgccattgcg atcgatgaat aatcgaatgg ctttactaga agacgaaact gcgctactcg
     4201 ctttaggtcg ctctagctct aattgcatat ctttgacaaa ggttgtggta accataaaaa
     4261 aaatcaacaa aatgaataca atgtcgatca acggtgccat atcaatcgct gtctcagttt
     4321 caggcggttc gctatatctc ataatgtgat cttattcata atatctatct tattaagtca
     4381 acctacataa caatttttta acttgaagca aatccttctc gatttgctgt tgtttcctgc
     4441 tcaaaaaact atgtgcgagc aaccctggaa tcgccactgc caaacccatc tgggttgtaa
     4501 acaaggcttg cgaaatacca ccggctatac cgcccgtttg agtaaataaa gccatactag
     4561 ccaacgaatc aaatgtttca accattccga ttacggttcc taacaagcct aacaagggag
     4621 aaataattac gatgattttg actaaaacag aaaatcttgc gatctcacgc tcaaactcat
     4681 aaaaagctgc gtcaagctga taagagagat actggctctg cttttttttt agcgcaacgc
     4741 ccttttgaat ggcctgctcg acaatacttt gcgcatcccg aaactttccc tcacgataaa
     4801 aactgagtaa gtcccttaca cccatttttt ggggtgtttt taaaacccag aagcgataac
     4861 caaggccgta ccacagtaat aacacacagc aaaacaaggg cggcatgacc agccctccgg
     4921 cttgaaataa ttcttcaaca agactaacaa tctcactcaa tgtcatgcgc caaccttatt
     4981 ccagcggtta gattggctgt ggttgcaatc taaatgactg agtttcagga tcattcccag
     5041 ccccaggatc tgtcgcgact gaattacccg attttccacc tagaaaggca ttggttgccc
     5101 ttaatgcaga gtgttcgata tcacgtcgaa tattccttgc ccaagcatta agcaaggaac
     5161 caagcattag tgttggaatt gctacgatca atcccaattc ggtcgtcact aaagcaattg
     5221 aaatcccacc tgaaagtaat ttgggatcgc ctgttccaaa ttcagtgatg acatcaaatg
     5281 ttgtaatcat tcccgtgact gtacctaata aaccgagcag cggtgcaacc gaagcaatga
     5341 ccataatcgc cgaaccaaac cgatctaatg gacccgcttc ttgcagaatg gcttcatgca
     5401 caatatcttc catatgctct ctatcatctt taagatgacg aagcgtggag gctaaaacac
     5461 gagaaatggc agatccgcca ctctcacata tctttttggc ttcagccaaa cggccctcag
     5521 cgataagtgc aataattcgg tcggctagtt gcttattatc cgtactattt tttcgtaaca
     5581 aaaaagcgcg aatgaaagcc aataaaagtg cgagagcgcc caggccaacg ataacccatc
     5641 caatcaaacc acctgattca ataatgctta gcactgtttt atcaggggtt tcgtcaatcg
     5701 cagtggtacg ggattcaaac aaaaatagct tcaaataatc cggctgctca ttgttattca
     5761 aggcggttgc aatctctgct gcaggctctt tccatacttt gaactcacca tttccagcgg
     5821 gaactaaggc tcctccccca tccgcactga caccaaacgc agcaatattg cctaactgga
     5881 taatagaacc ttctgcttgc ttaccagatt ctaaaaagaa ctttcccgcc ttagattgta
     5941 tttctcctaa tttctgaatc agcgtaaggc cttgctggag taagaaagta gtcttttcta
     6001 gatcattttc ctttccttcc ttaattggag ggggcatctc aatcccatac tgcttcaagg
     6061 tcagctccga ctgagcatag gttatttcca aagcatcgtt gcgctcactc accgccgcct
     6121 gagtccgctc agcttctaca atttggctat tgaactgatc gatttttgcc gagcgtgaaa
     6181 cagattcacg ttccagcgca ttaatttttc gaatcaactc ttgttctttg ctattagcgc
     6241 tggctttgta tttatccagc tgcgctttca attctcgctt ctgcgcatcc agaaacgcat
     6301 actccctttt atacgcagtt tcgaggttaa caggtttcga atctgaggtt gtgtttttct
     6361 tagctgatgc tgccttgcct ggattattgg tattcgtttt tttatccgaa gcaggttcag
     6421 tagtaattgt ttccgttgct tgcgacactt caggcgtcgt ttcctgcgcc caagctgtgg
     6481 aaatcaaata acaaaacaac ccgactaaaa gaaatggttt cataattttt tcaaaggatt
     6541 cggtaactca aagtaacctt ggcgtatttg tttttttaat gcatcgaaaa gctgactgat
     6601 ttgagtttta tctttcttag tatccattga aacaaacttc caactcgatc cttcagtgtg
     6661 ctgcgccata ccaaaccgcc catcacgcgt ttggaaataa agaaaaatgg ttcccaattt
     6721 ggcaatatcc gccagtatct tttcaccgtt taactcgatc gtttgctgat aaataccatt
     6781 ctctttactt aaccgcacct catcttccag caatgaccaa gcatgattga ccgccttttt
     6841 aggatcgata agcttattgt tgagattgtc ttctagctct tgaatggctt taagtcggtc
     6901 ttcaatctta aatggcaagc ctgcttgtat gaatcgctta tattcagcca aaaatagaat
     6961 caagcttggc tttaaattat caccagaatg ttcggcctgc ttagattctg cagttaattt
     7021 ctcaatcgat tgctgcagtt tttctatgct gattttctgc cgtctttcct ctacgctcaa
     7081 atctgctaat tgagatgaga gagaagcgat ccgactatta tggttttctt tctctagatc
     7141 caactgagat tgcaactcct cgacttcacc gcgaattttt gcgagtgatt tcgctaaatt
     7201 ctccaagttg tctgcagtaa cggtgggtgc atacaacgta atgatactca gcccaattaa
     7261 cgtcagcagt ccctcaaaac attttttcaa tagatatagc attgcaaaaa acctcaactg
     7321 gaattgtttc acttggtagc agaaaatcga taatggtctg ctcagtattg gcagcgccaa
     7381 atcgattatt tccgttatga atgtggtcca acaagctact tagaattgct agtatcgtac
     7441 tgggcgaatg ttacattttt atgtcaatca cgccagaaag ggagtttaat cagggataag
     7501 aatgaatgat cagtcgctat gatttagaga aattgctggg acggctaaga tcgcttgaaa
     7561 atctaccaat ctgtcatgaa gcggtaatat tcaaccgtat aatgataact accaagtgct
     7621 tcccttttct tggtctgggt accggtgctt ttcccctgtc tgtttcatat tcgcatccgc
     7681 actggtaccc gtcttacctc attttaggat tgacctagtc tagagcctaa ctaattcaat
     7741 tctgaatgat ataaggaaca aattagtaat aataaggcct ccaagaatca ctagatctag
     7801 tgaaaacaaa agaaataaca aatgcggtat tgcaagactt tcctctgttt acagtagttt
     7861 atctgataga cgaaactata actatgaaaa cagttgctaa tgaatttggc gcgtaaaatt
     7921 cgcctatttt ttccttttgt ccttaatatg ctggttctgg gtgtctactt cgaacaaacc
     7981 aatggcttta attaggtcac tgaattttgc gtaaccataa tttcgcgaat caaatgccga
     8041 gaagtttttc acaatatgtt tcttcacttc tcccatgtaa gcaaaaccat cttcatcgga
     8101 aattgcttca attgcggagc ggatcagcga taccagtttg gtatccattg ccagttccct
     8161 ggaatttttt ttggtgccag gtatggttgt aacttcgctc gaaaatgata ctgatttagt
     8221 agttttcttt tttaggcttg ttttggattt gttaggaata tcttccgctt gtgtcgcaac
     8281 actatcacca attccgatat gcttgttttg cgagagaatt tcgaagtact tgaattcatt
     8341 gcaagcagct tgcagtgatt ccggtgtctt ttcctcacca aaaccgtaga caagtttccc
     8401 agattctcgg aatcgaattg caagcctagt gaaatcgcta tcactggaaa cgatacaaaa
     8461 accttccagc ggggccgtat agagtaaatc catggcatca atgatcaatg cgctatccgt
     8521 tgcattctta cctttggtat tggcatattg ctgaattggc tggatggcat gtttgggcaa
     8581 gatttccttc catcgactca actggttttg agtccaatca ccataaatac gcctgacact
     8641 ggcatcgccg aattttgata tttcctcgaa taattcatca atgagatcga tcaccttaaa
     8701 ggtgttatct gcgtcaatca atacagctag cctcggatta ttgttgtcgg cagtcattga
     8761 tcagcccaag ataatcaaaa tttgtagtgt acatttagga tagttactta aatcatctga
     8821 agccagaagt atatagaggt ctttgaaaaa tcagataaga atctcgtttt attgtaattc
     8881 ggctagagtg aattgaatat accagatatg gattctgaat cttagtagca ctagatgata
     8941 ctgaagctca tgatgaagtc agaaattatg agccccagct atttgaacga ttagatgtag
     9001 cagtgcttaa gtaccaataa ctccaccatc atctttagtg atgacgatca ccgatgaacg
     9061 tggtttagat acgacagcgc cggtaaaagt ggtagggccg ttgtatccgt tgtccggcca
     9121 gcttgaatta tcagtaaagt tgatcttcca atcttcaccg ggatgttgga taccaacaaa
     9181 catgctacgg ccatcagggg tagtcaccac acccgttact tcacagtttg gtgggctggt
     9241 taagaaacgc ctggtctcac cggtttgtgg attagcacac atcatgacat taccgccgat
     9301 attgatccaa tcgccttttg catcaccttg ttgatcggtt tgaatccata agcgaccgtc
     9361 tttatcgaac cacaaaccat ctggcgcacc gtagtcatca ccgttaatat tccctttata
     9421 accatcagag cccgcagggt tgtaactcgc tcccagagct ttggcggagt ctctatcacc
     9481 acattgcaca aaaatatccc aagaaaagtg cgttgcggta acatttgcat catcttcgcg
     9541 ccaacgaatt atatggccat agtcattatc tgggcgtgga ttagcagcat ctaccgaagg
     9601 attagcgcta ccagcattgc tcgtaccatc aatttgatta ctcgacgcag gagtattgcc
     9661 gcggcggctg ttattggtca gtgtcatata gacttcgcca gtttccggat gcgctgcacc
     9721 ccattcaggt cgatccatca tcgtcgcacc taaacgatct gccgcttgac gggttttaat
     9781 caacacttct gcttgactgc caaagccatt ctcaggagtc aatccatttt ggccccagat
     9841 cagcggcatc caatcaccac tgccatcggc gttaaatctc gcaacataca gcgtaccttt
     9901 atcaagcata tcgggatcgg tcacctgcgg attgccattc cattttttgg cacagacaaa
     9961 cttataaata tattcattgc gttcgtcatc gcccatgtaa aaagcaacgt gcttctgccg
    10021 atcaaccaca acttgagcac cttcatgttt cattcgccca agtgcagtgc gctttattgg
    10081 cttatcccaa gggctgcgtg gatcgatctc aaccacccaa ccaaaacgat gcggctcatg
    10141 aggatgtagg tcagcacgga accgttcgtc cacctcgtgc caacgataac caaagccacc
    10201 ttttactaca ccgtaacgag actgaccttt gagaatgtta agttttcggt caagatctaa
    10261 ttcaccgatg acatcaccac tctcattaga aaaatagcca ttccaatttt cttcgcaggt
    10321 taggtaggtt ccccaagggg tataaccatg tgaacagtta ttcaatgtac ccagtacatt
    10381 tttaccggta gcgtctgcag cagtcttcat aagctcatgc cctgccgctg gaccactgat
    10441 aacacaaggc gtgttgcctg tgatgcgctg gttaaaatac caattacggg aaagtgacca
    10501 tttcttccgt ttcttttcta tcatgataat gttgacacca tgcgcagctt gctgagtacg
    10561 aaccattgca agggtgatcg gaactttggc ataatcgctt gcaggggtaa tattgttgac
    10621 taatggaggg tcacaatatt cactattact cactaataat ccacgctggt tcccagcctg
    10681 ccctggccag gggaaaaaat gcataccatc cgtatgggcc ccataacaat ggagctgtgt
    10741 ttcctcatcc attgctgaaa ccgtatccca gtcaggttta tcgttaatgg aatcacccca
    10801 ggatatcaac actcttgctg tataaccctc tggcactgtt attccatcaa tcatcggttg
    10861 caaattaggg cttactgggt taaaacccaa aagtaggcca tgcttagaaa tatcttcagc
    10921 aaaacttgcc atactttcca tcgctgcagt tcccaggagg gtcattgtgg tgattcctgc
    10981 cgttgcagtc acggatgact tgagaaattc acgtcgtgat atttttcttt gttcgatgat
    11041 ttccatcatc gatggattac tgctctcatt acaggttatg tcattaattt tgagcttact
    11101 atcgggatgc agtttgctac tcatattacc tccactttta atgtaatttt cgatacagca
    11161 aacttagttt caaaaagtga cagtttgata acacctcgat taattttttt tgatgatttg
    11221 atgactaatt atcaaagtgt cggtatcgaa aagttgcagt tagtagcagt ggagggtctc
    11281 aagccaagaa agctatgatt taatcaaaaa acaacgcaga ttgctgtaag caatttagaa
    11341 acaatcttgt gaggtaacaa aaaaactaag ggatcagctt gactgtcatg aagcagtaac
    11401 actcaactgt ataatggtgt gtactaagta cctcccttct gttgctctgg gtaccagtgg
    11461 tttctccctc tgtgggtata ctactcactt tacactggta cctgtttttt cttcgtatct
    11521 tcatatcatt tcgatgaaaa taacttgatt ggctaaaata accaaaatgg cgactaaaac
    11581 gatattactc ctaacactct ctaacaaata tttaatgtaa ttgacatata tctgacatca
    11641 atagccttta gtatcggcgt tgtataaatt caaccgttgt ttcgaattta tatccacatc
    11701 gattcttaca aacaggatcg tttatgtagc gtacagatat tttcctttat attaaatgga
    11761 gatctaaaaa tgaaaaaact ttcgttaatt ctcgtcataa caacaatcat gctggtatct
    11821 agctttaatc taatggccgc atgtgctccc tgggttcaaa ctagagtcac acaaccagac
    11881 tatgctgttt tagacgctgc tttaacttcg gctatccaaa cccgcttaaa aaataagcta
    11941 aatgcgacta aggatgaacc aacttatagt gctttacgca ttgaagcaga aggtttagct
    12001 gcaacgattg caaatggccg agtcgtcatt acaatgccgg atggtaccgt agtagttgat
    12061 acatcaaaag gggcaaataa cacttatgat aatttcaaag ctaaaactat taacgaaaac
    12121 cacaattctc gaatcgctat tctacatagc caactctatg actgtggttt aggccttgaa
    12181 accaaactaa gtactactga taacacaaaa gagaattatt tagctaggcg cttaggcgca
    12241 tacttgaata gcgccggaac agttagactt tctcaaaaac tgtaaaatct aaccaacaat
    12301 caattccttt aattgggtag tgccgcgact acgaggttac atttttgtgt gcctcgtagt
    12361 ctataactac cctatcactc agaaaatatt cttatttacc tcgttaaatt ccccaataag
    12421 gagccactaa cgcttcttct gtagtttctt tatacagaat cgttgtgaag aaaaacattc
    12481 gctagcaatg gatgtttttt caatccagtt tttttacagc cttttcagat aactctattc
    12541 ttaaaaaatt aacaaagctg aaattgttca gacataaacc aaagctaaag tgtttataca
    12601 gtgcactgtt tccattcgtt ttaacttgta atcgctttac tttttagaaa aggagaatct
    12661 catggatttt gaaagaacca tggcaatcat tgcatcctgc atgttatttt caattacctt
    12721 tagtatggta accaaaaatt cacttgctgc atcggatcac ggtcagaaag acattggtca
    12781 agtaaaagtg agtgaaatta aaaaaaccaa tattcaatta ggacctcgcc cattttttct
    12841 ggttgatgat atgagcgaag gtgccttaaa aaataaatta aaatcatgcg ccaataaagt
    12901 atcgagaaaa accgaatttt caatcggcca ccgtggcgca gcccttcaat tccctgaaca
    12961 tactaaagaa tcctatttag cagcggctcg aatgggtgct ggcattattg aatgcgatgt
    13021 gacgtttaca caggatcgtg agttagtctg tcgccattcc cagtgtgacc tgcacacgac
    13081 gactaacatt ctcgcagttc ccgagcttgc cgctaaatgc tcagttcctt tttcacctgc
    13141 tgagattgac ccaataacgg gagaaattat taaaccggcc tccgcacaat gctgcacaag
    13201 cgatatcaca cttgcagaat ttaaaatgct caacggtaaa atggatgcaa gcaatcctgc
    13261 tgctacaact cctgaagcct atttgggcgg aaccccagct tggagaaccg atttatatgc
    13321 aagtaaaggt accctactta ctcatcgaga aagcatcgaa ctttttaagc aattaggtgt
    13381 aaaaatgacg cctgaactca aagcacccag cgttcccatg ccatttcaag gggattttac
    13441 acagcaagat tatgcacaaa agttaatcga tgaatataaa cttttaggga ttaagcccga
    13501 gaaagttttc cctcagtctt ttagtttaag tgatgtactc tattggattc agaatgaatc
    13561 tgaatttgga gcgcaagcgg tatatctaga tggacgatac gacgatccta cttttgatcc
    13621 tcaaaactct gctagttgga acccgagtat gtcggatctc gtttcacaag gcgtcaaaat
    13681 tattgctcct cctatgtggg tattggtgac agtagaaaat aaccgcattg ttccttcagt
    13741 atatgctaaa gctgccaaag aagcaggctt agatatcatt acctggacca tcgaacgctc
    13801 ccctccacta attgcaaatg gtgattggta ttatcaaacc atcgatagcg ttgttaataa
    13861 agatggagat caatatgttt tattagatgt acttaccaag caggtcggta tcttgggaat
    13921 attttcagat tggccagcaa cggtaactta ttatgcaaat tgtatggata taaagtagta
    13981 aactaacgga gcaggcaagg atggggaagg ctctccatcc ttgtggtcaa atgatatcaa
    14041 aaatttttac ccttaacttg tgctctcgca taactcatgc acaaaaaaac ctaatccttg
    14101 agccagctta tcagtttcaa cgaagaaatt tttgtttaac acaacatctc catgatcgag
    14161 tagcgatgtt aatatcaagt gacttttctt acccaactgc gctaagcgct tatgcaagaa
    14221 aattgactca tgactcggga tcacttgatc atgataacca tgtatcaaac taattgaaaa
    14281 atcgatatgc gtaagctgct tatctaaatc aaatcgattt aaccaatgtt ctcttcgatc
    14341 gatttcttct aaagcacttt gggctacttg atgcctaaaa aaggcgtctt ccaataattg
    14401 acaaaaaaaa tgcgttgctt ctttagaagt attttgtaac gaataaggta gcaatggatt
    14461 ttttttctta aaaccattat ctgccaccgc aatatcaaat agctgagtta cttctggatt
    14521 tcgatatgag ctttgagaaa gtaggttccg caataaaata aagcgtgcat attcgtcgat
    14581 ttcctcctga tccaggacaa atcggattac attcctaaaa tctgcaaatg taccaattaa
    14641 acacatcgct tttatggagg atgaaatctg tggatgcgaa gcggccaata gcgcaacccc
    14701 gcctgaatat gaagctgaga aaatacctag ctgacggtgg tttggattca actccgaatg
    14761 agtcgcaatt gcctcaatta acgctgcaaa cttactgatc gtcgatgggt gaatgataag
    14821 ctgttcaata tccgaaacgc acggaataat cactcgaaaa cccaagtatg aaaatgctct
    14881 agcaacagca tccatccgat gatccctgta cccatgaaca gacaaaccag ggaaagcaat
    14941 aatggtatga atggaatcac cggggggctc gtacactaaa gcagaatgca tctcgtactg
    15001 cgtgctaata ggctgttgca agatgctaat tcttacccat tggggtaaat aacggcgcaa
    15061 cagaaaacga taacctaatc gccgatacaa tatagacttg cttctattat ggtccaaagc
    15121 aaattctatc aagcaatcat tcgtagcatt tcctttacac cggaaccagg taatcggcaa
    15181 accgcttcgc agcagactcc caactaaatg tttctgctcg ttcacgtgct ttttcacggg
    15241 gtatctcgaa tgcgctgagt acagcttctt ttaaatcggg caacatagca cctgctccac
    15301 tatcaccaat gacttcaagc ggcccatcca ctggaaagca agcgactggc gtacccgttg
    15361 ccatcgattc cagtaaaaca agaccaaacg tatcagcagc gctcgggaaa acaaatacat
    15421 ccgcgcttgc ataaagctga accagttcgt ttctgggtaa aatacctaac caaagaacgt
    15481 tgggatagcg tcttttaagg tcactctcga gtggccctac cccactaacc actttactcc
    15541 cgggaatatc caaatctaga aacgcttgaa tattcttttc atatgaaatt cgacctacaa
    15601 ataaggaaat cggtcttgga agtctcccta acgcaggaaa ttcagccggt ttctcatgga
    15661 atcggaacaa ttcagtatcc acaccatgtg accaagattt aaggttttgg aacccacgtt
    15721 tctgaagacc ttctagtacc gattgagtag gtaccattac ccctgaggaa ggttggtgga
    15781 aatgcttgag cacggtatat cccacgaaca ctggaacacc cagtgacgcc ttgaaaattt
    15841 caggaaattt agtatgaaag gcagaagtaa aaaaccaacc ttctttgagg catattgatc
    15901 gcgctgccca gcctagaggc ccttccgtta caatatgaat tgcatcagga ttatattgca
    15961 ggagtagctt ttgcaaatgt tttcgtggcc gaatagcaag atcaatgccg gcataaccag
    16021 ggcaaggtct ggtattaaat aagcctggat gaataatcat aaattggtga tcaaattttt
    16081 caagctctgc cacaagttcg actaaagtgg ttacgacccc attgacctgg ggctgccagg
    16141 catcagtaat taaagcaatc ttcatgcaag caccttttcg ttatccttaa tcttatttgt
    16201 tactacgggc ttacctatcg gtaagcagcc ttccgttagc gggatttcta ccgtattggt
    16261 tagttgcagg gcatgctgag gccagtgaat cagctcgata cttccatcaa aatgctctac
    16321 taatgcggtt agactttcaa cccagtcacc atcattacaa taagtaacac cattgatgtc
    16381 acggatttca gcgtgatgga tatgtccaca aatcacacca ttgagtcctc gtcgacttgc
    16441 ttcggccgca acggcttgtt caaaggagag cacataattg accgctgttt taactttata
    16501 tttaaggtac tgagaaagcg accaataggg tagacccaat cgagctcgca gcgaggttaa
    16561 gtatcgattc aacttcagtg taaattcgta gagattatca ccgacgtagg caagccattt
    16621 tgcacactga atgacgccat caaaataatc accatgaatt acccaaaatt tgcggccatc
    16681 gactgtttca tgaactgcct cctcgacaat ttcgatgcca ccaaacgagt gtcccaagaa
    16741 ttcacgtgca aattcatcat gattacctgg cacataaata actttgcagc ctttgcgtac
    16801 cttacggagc aatttctgta tcacatcgtt atgagcctga ggccaatacc aacgccgacg
    16861 taactgccag ccatcgataa tatcgccgac cagatacaac tgctcgctcg agtaagtttt
    16921 caaaaaatct aacaatgctg aggcttggca acctgttgtg ccaagatgaa gatcagaaat
    16981 aaaaatcgca cgaacgcttg atttatagtt ttcttctgga gagtcatcca gttcactgtt
    17041 tgattgccag ttatcagtgg gtttattgaa ttggttatgc aaataagcgt aatgatcgcg
    17101 catacttacc ccaagaagtg tttacgataa cgttcgggca aatttctcat gcgcattagc
    17161 atcggcaaat ctgcagtatt gaaatccggg tcccaagctg gggcgcctaa cactttggcg
    17221 cctaccctta gatagccttt gactaatgcg ggaggatcaa tatcgagcgt ctgatctaat
    17281 tgctcaaccg gtaaaggtaa acgtggaata acctgtaatt cttgacttgc caggtgactt
    17341 tccttcatct gccgccaaat acttgctgct gcatatccca tcccaatttg accatccttt
    17401 acctgcatat tcatgggaat actgacgcat ccgacaatgt catccagctt attttgcgtc
    17461 atgaactgaa cgagtgcttt ccaaagtgcc agaatgacat taccctgacg ataatcctta
    17521 tgtacgcagc tacggccgat ctctgcaaga tgctcgcgca acggttgcaa cgcacgtaaa
    17581 tcaaattcac tatcactgta taattttcca gctaatttag cttgtgcagg tgtcaaaacg
    17641 cgataggttc caatgacttg ccatgtttta cgatcccgta ccagaagatg ctcacaataa
    17701 tcgtcaaaga tatcgatatc atagcctgat aaaatattag gaaggcttgc acccatctca
    17761 gttgcaaata cttgatatct taatttttga gcttcgcgca cttccgataa gtgtcttgcc
    17821 caagtcgtta caatattggt ctgactttcg agctttgttg tagcacactg gcctacctca
    17881 gggatactac tggtgctatc tctaaaaaat gaatcttgca gcatctctgc agtcgccatg
    17941 atcgattcac tcattactga ttcctccaaa tataaagaaa ttaagtcgta aaactcattg
    18001 aatctctaca tatttttatc gaatcatgtg atggcgctat gtcagtttta tgaagttctt
    18061 gttaccgcca ctgagttgct cttttttttg ataaggtgta atgattcgaa aaagaaaatt
    18121 taaggcaacc ctaacggttc aggattactc tcgtaggagc tcagcagcca aagaaacagt
    18181 gactttcttt gagaaattga attgttcatc tactaatgaa aaagccccta cttagctaca
    18241 taagtaaggg cttcttccag taagaattga atctgctagt ctttagcgat ccataacgct
    18301 tagaaaggga aagtccattc ttgagtccag ttatcattgg cgtccctaaa cgcaccggca
    18361 taactaactg gcttaaagta gctatcactc aatgtagcgc ctgcttgtaa cactggtgag
    18421 cctgcattag gtaagaagcc atttaataat ggatcctgtg ctttattccc ggtttgtgaa
    18481 ttaaaccagt ctgctgtaga tgcagttgcg ccacctgatt ctgcgaagct attctgacac
    18541 ccgtttacaa aggaattatt gatgctcaat tcactcgctt gaccgaccaa cgcagatgtt
    18601 gccgtaccat caagcgttaa gcagttttga aatccagtaa atattgaatt ccaaatattc
    18661 acgcctgtcc cacgtcttaa taaagcacct gacgcctgag aaacaggaga accgatgaga
    18721 gtcatatttg ataacacggg ttttgagcga ggcgcaatat ctggattatt cggattattg
    18781 tcggcttcga taccatgatc accatcactt gctgattgtt gaatcagtac gaattgtgcc
    18841 ttacctctcc aacccagccc ccaatccaga ctatcatctt caatgccact cagcacgata
    18901 tgcttgagat ttgcagtacc accaaacagc tcgataccat catctagccc acgatgaact
    18961 tgtatgaatt gaagcacggt tccagatcca actgcattta gtgttaaccc attgagctct
    19021 tcatcgggcc gaactgcaaa gccggcaaac aaaatttgca catatttcat gattccgctg
    19081 ctgtcagcat cattattacc acctgaaaat tcggtcgtaa tggcttcatt cgcaacttca
    19141 catacggcag ttccttcatt acagccattg atacgcgcaa agccatttaa tacgactccc
    19201 ccccattcac cggcagcttg ctcattgggg cctgagaaaa caataggatt atcaggcgta
    19261 ccttctgcta agatcttaga accacgtcta atccaaagat aatctgctcc ttgctggcca
    19321 acgatttttg taccgggatt aatcgtaatc gtcgcatttt ccgtcttatc acccccaata
    19381 aaaacaccac ccgacaaaat ccactgggta tttttagtca acgtaatatt gcggtttctg
    19441 atttcaccac tcaatataca gctattagca atcgggcctg gtgtcgcaaa atctggacaa
    19501 ctctcaaact gattattcac gagctgctct aatgaaaagc gcagcggatt tgaacctgga
    19561 atcaatttca atttagcgta aaattccgca ccccctgcgt cgaccgaggc gatatgaaca
    19621 gcagtagtcg ctgaatcata cacattgcgt tgcacatttt ttgcgggtgc aatcggttgt
    19681 gcatcaacta ggacaagctc attaccattt acaagttgta gcttggcact gaaaaaattc
    19741 gatgttgcac catctagcac ctcaacgata ggtaaatcaa ccgtattatc agatggatta
    19801 taggtagcgg ttgctgcttg cacattgata gcgaatgcac tcattacagc ggctgtgatc
    19861 gcggttagtc ggatatttaa cttactaaat ctagtcataa cttacttccc tcatgtttaa
    19921 aaactcgaag gaaaaagact agcaatcaat gatgaattta atgttacaga aaaatgaaag
    19981 atttgtgacg atcaatatta agtaaagcca taatagtgat aagcgccctt aatgaaacaa
    20041 taggcgcaat ttttgtaggg tgcagcaaat ttgagctaag tgctatcttc aaattggttt
    20101 aacgaataaa caattttggt actttttatt gcggtaattt tcaagtaggc tgttgacgat
    20161 tgctgctaac atcgtttgga aatgaaaatt tcaccgtgtt atcgcgttaa atatcgcaga
    20221 atatggaagc aagccaacct gatactaaaa atccaatctg gatcgctcga gaaacgctaa
    20281 agcatttagc gactcagcgt attgcgccaa cccccgatca gtatcaagca atttattatc
    20341 agattgctgg cataaatcca cccaagcatg atgtgtcgga ttcattagcc aataatttat
    20401 tggcaatact acgaacgctt agcttacctc aggcagcaaa tcgctatgtg gttgatatta
    20461 atttagcagt cggcaatgaa aattggccga ccgttgctca attggttggg cagctgattc
    20521 aattacaagc atcgcctaat cagcaactag agcgaccatg ggaagaatta ctgctaatgc
    20581 tatgggacgc acgtggaaaa gccagcgacg accatactcg acgtgatgaa ttagcgtata
    20641 ccgtagcact atttgctggt caacctgaaa aacttaatca cgagctcaat accttaatca
    20701 cgcactggct gaataagccc catgaaattg aggtatttga taatgaaaat tctcctgttg
    20761 aaatcatgcc aatggatagt ctggcgcagg aaaattgttc acaaacccag tatccgtggc
    20821 aacaatggca agcgctcgtt gttaacacat tgcgcgatgg cctcattcct cgccttactt
    20881 ctcaccagca ttggattgat gaaattgaat tcatcatcca agcgattgaa gccatcgaac
    20941 aacctgaaaa tatcgactgg atacagaaaa gacttcgaca gtgctggatt catatggcgc
    21001 tgcgcatcga tcaagaacaa aggctgatcg atggactgtt aagcgtactt gcgctattcg
    21061 tagaacatct atcacttcca tcggatcttg atccctgggt aacacatcaa cttgataaca
    21121 ttcgacgaaa tttagcaaag ccactaacgc tccatcgcat ttatgacgcg gaatcgatac
    21181 tcaaggagct agtttatcag caagggctgt tacagcatac cttgactgaa gcgcagcaaa
    21241 cgcttaaagc catgatttcg gtattcttag aacgtttagg ggttgtaaca gaagcaacgg
    21301 gtgaatttca gcacaaaata gatcactata cagaacgtgt tcaccaaacg agtcaggccg
    21361 ttgattttaa gtcgctattg gaagagctga tggaagatac tcgccatatg caactcgaca
    21421 tgctccgcgc tcgggatgaa ttagtaaaaa cgcaacaaca agcagatgct gcacagcagc
    21481 gtgtcaacga attacgacag cagttgcgtc agatcagtga gcataatcgc gacgatcggc
    21541 ttaccggtgc gttaagtcat cgcgccctag tcgaaacgtt tagtgcagaa caaagccaca
    21601 tgtcgcaaag tggtaaacca ttatgcttag cgttgctaga cgtcgataac ttgagtgaat
    21661 taaatgccca attaggccgg caggcgggcg atgacacgtt aaccggtttg gtccaagtga
    21721 taaaaacagt aataagggag ggtgacagcg taattcgata tggctctgaa gaatttctca
    21781 ttttgctgcc caatacgccg ctaagcacgg ccaccgattt attacggcga ttacaacgtg
    21841 aattaacaaa acgcttcttt atgaacaaca ataaacgctt gttgataacg tttagtgcag
    21901 gagtcactcg atgtctatcg caagaaagtc gagaacaagc cttagatcgc gctgactatg
    21961 ctgtttttct tgcgagaaaa caaggtaaaa accaggttgc tgtagttgaa gctcccgaaa
    22021 tacctgtaaa ttaggcccga tgcacctcta gctagccgca atttttaacc atttctctgc
    22081 ctctagataa tctgccccca tctcttctag accagaagaa tcacaaattc acactagctg
    22141 cagagtcggt gtcgaagtag cacaagccgt taaaactgcc atacagctaa taactaatat
    22201 tattcgcatg atttactttt ccttctcctg atatatagcg tggaatggcg ctacactgtt
    22261 cgatttgctt tcaatgtcct aattttagaa aagtatagca gcaagaccgt cacaggctag
    22321 taatattatg ttagttgttt ttttgctacc tgatattatg aggtagtagt cgagagaaaa
    22381 tattttctgg tactaagaga taaattttaa ctgttgaaaa tagcaaaaat atactgccat
    22441 taccttaaaa tcttgagaaa aactcagcgc ctcgataaaa aaatacttat ttctaatctc
    22501 atgcacagta agttttatta gtgacctccc ccaagttaag gagtgtacag atgtttaccg
    22561 gaattatcga agcagtcgga ataattgatc aaatctcaca aaacccaaaa ggagttagct
    22621 tacagattga tgctggaaag cttgatctga gcgatgtcaa gatcggtgat agcatcgcgg
    22681 ttaatggtat ttgcttgacg gtcacgaaac agggtgaatg ttactttgct gtggatgttt
    22741 ctcatgaaac attacgctgc accaaaggtt tgaagaatct aggtagtcag gttaatctcg
    22801 aaaaagcaat gcggctatcg gatcgtttag gcggacatct agttagtgga catgtggacg
    22861 gcgttggtga agttatacaa ttcgaacctg aaggggaaaa ttatcgactc agcattcaag
    22921 cgcctgatgc gttgctaaaa tatatcgtgg ttaaaggatc tgtaaccatc aacggcgtga
    22981 gtcttacggt taatcgtatc gaaggaaagg tatttgatat taatttaatt ccccatacat
    23041 tagcggaaac caatttgaaa ttattgaaag taggtgaggc agtaaatttg gaaaccgatt
    23101 tattagcgcg ctacgtcgag cgtttaatga ctcaatcgat caatcaaatt taggtacgac
    23161 tatgacgatt agtagcatcg aaacaatagt aagcgatttt aaggcgggca aaatggtcat
    23221 tctggtcgat gaagaagatc gagaaaatga aggcgatcta gtcctggctg ccgagtttgt
    23281 tacaccagaa gcgattaatt ttatggcaaa gtttggtaga ggactaattt gtttgacgct
    23341 gactgaagag cgttgtcacc aacttaaatt atcaccgatg gtagcagcca atcattcatc
    23401 tcacgggact aattttactg tttcaatcga agcagccagc ggtgttacaa ccggtatttc
    23461 tgctgctgat cgggcacgaa ctatccaagt cgctgttaaa aaagaagcta aaccggagga
    23521 tattgttcag cccggtcaca tttttccgct taaagcgcag aaaggagggg ttctggttag
    23581 agctggccat actgaagcgg gttgtgatct cgctcaaatg gcaggactta tgccagcagc
    23641 cgtaatttgt gaaatactca aagataacgg tgaaatggcc cgactgccgg atttagtgga
    23701 atttgcgaac acgcaccaac ttaaaattgg tacaatagcc gaccttattc accaccgcag
    23761 tttaaccgaa agtttggtat cgcgtattgc ggagcgaacc atacaaaccg tttttggtga
    23821 atttgatttg aaagcctatc acgacaaaac tgcgaatatt acacatcttg cgttagtcaa
    23881 aggacagctt cagtctgaaa aagaaattct ggtgagagtg cacgaaccgt tgtcagtcat
    23941 tgatgtgttg gacattgctg actgtagcca ttcttggagt attaacgatg cgatgcgggt
    24001 aattgctgag gccggttcag gcgttatcgt tatgttacat cgcaaaggcg acgctggaat
    24061 cttaaccgag cgcattatgg caacaaaatc ttggtatcct gatcagatca agactgattt
    24121 acgcgatcat gggattggcg ctcaaattct gaaagatctc ggtgttcgta aaatgcgttt
    24181 actggctacg ccgcgcaaaa tgccaagcat gaccggattt ggtttagaag tgactggtta
    24241 tttagaatcg agcaacaagc aaaccgctac ctgaggatac gtgtttaaaa aaggcacata
    24301 ggaattgaat tagcatgggt tattacgaca acataccaga aattgaaact aatctagatg
    24361 gctccgaatt gagaattggc atcgtgatga gtcgctttaa tattgaaatt ggtgaaggat
    24421 tattaggtgc ctgtactgct gagctggtac aacttggtgt gcaagaagcc aatattcagt
    24481 tagtaaccgt acccggtgca ttagaagctc ccgttacgct acaaaaaatg gcattgtccg
    24541 atcaatttga tgcactcatt gcattgggtg ccgttattcg gggcgaaacc tatcattttg
    24601 aagttgtcgc taatcaatct gcaggcgggt taatgaaggc tcagattgaa acgggcattc
    24661 cgatcatcaa tgcgattctc accacggata ccgaagatca ggcttcgtca cgaatgtctc
    24721 aaaaaggaaa agacgcagcg agagcagcca ttgaaatggc taatcttttc gataaattag
    24781 ttgaatttga atgaaaacag ttgccatgaa agaagcaccg gccaaatcag gcccaaaacc
    24841 tgctcccaaa ttcaaaaatc gacgccgctc tgcacgagag cttgcgctgc aaggtatcta
    24901 tcaatggcgc cttaccgcaa gcgaagctgg ttttatcgaa gcacagctga gaagtgcaag
    24961 cttcttcgca agagttgatg cggttttttt ttctgattta ttgcaaggtg tcatcaaaca
    25021 cgctgatgaa cttgagcaga aaattgcacc ctgcctggac agaacgttcc aggaacttag
    25081 ccccattgag ttgtcgatac tcttgttgag tacgtatgaa cttatttatc accccaatat
    25141 tccctatcgt ccaattatca atgagtcaat cgagcttgca aaaacttttg ggggaaccga
    25201 tggccataaa tatatcaatg gtgtcatgga caaattggca actcagctac gtggcacaga
    25261 ggttgcattg cacgcaaaga aaacagccaa gttccattga gtccaccata aaaatctatt
    25321 cacttgctca acaaaaatga cctgcacgat tctagaatcg cattacgatt agcaaaacag
    25381 tcaacaccat aatttagagc gtggaatctg aatttgatat cattcgtcgc tattttacac
    25441 gtcccacaaa aaaagctttg ctgggcattg gcgatgatgc cgcactgatt gcttataatg
    25501 caaccaccga tcttgctata tctaccgata ccttggtagt aggccatcat ttttttccag
    25561 atacagatcc tagtcaatta ggccacaaag ctttggcagt taatctttct gacatggcgg
    25621 caatgggcgc tgttccacgc tggcttactt tagcaatcac attacccaga gatgcaccac
    25681 aaactaattc ggcctggtta caggcttttg ctcacgggtt cttcgcgtta gctgaccgct
    25741 atcaagttga gttgattggt ggaaacacga cgaatggacc acttaatatt tgtgttcaaa
    25801 ttatcggtga aattcctaga aataaggcat taaaacgtag caatgcacaa ctgaacgatg
    25861 atatttgggt ctcagggcag ctcggtaacg ccgcacttgc acttgcccat ctcaagcagc
    25921 agcttgtttt aactagtgag gcgctcgatg tttgtttacc cgcactccat accccaactg
    25981 caagggtagc gctaggacag cagctaatcg atctagcgca tagcgcaatc gatatttctg
    26041 atggtttact tgccgattta gggcatatcc tttcaagttc gaatctagcc gctgtcatcc
    26101 agcttgagac cattccacat tcttgggttt ggaatcgcta tgcagcgcat ccgcaagccc
    26161 tcgaatggct attgtcaggc ggtgatgatt acgagctttg ttttacggcc ccggaaaccg
    26221 agcgcaaaaa aattcatacg ctctcaaaag aattagctac cccactgacg tgtatcggaa
    26281 aaatattaca aggagaaggt ttatctatag taaactcaac tggaacagta attgagcata
    26341 cgaaaaaagg ctatgaccac ttcctttcct gaaccgtatc gatccaattt caagcttcct
    26401 ccccttaagc cggatatagc ctttgtatat agccgcttag cacacttttt agcctttgct
    26461 gccggtgcag gactcagtaa atacgcaccg ggcacgattg gaacccttgt tgctttccct
    26521 atctattggc tgctgcagtt gttatttgat ccaatcgcac ttttgctcgt catcgatatc
    26581 tttttcatta ttggcatttg ggcgtgcggc atcaccggga aagcgctgca tgcacccgat
    26641 cattcaggta tggtgtggga cgaaatcgtt gcgttcttgc ttgttttata tttcatacca
    26701 gaccattggt tgtggcaaat cagtgcgttt atcttatttc ggttttttga tatcgtgaag
    26761 ccgccgccca ttaaatacta cgaaaaaaaa ttccacggtg gggtaggcgt aatgtttgat
    26821 gacttactcg cggctttttt taccctcctg tgtctagccg gatggaaatc gatcgtctta
    26881 tttgaaagtt atttttagcg cactgcaatg atcgtcgatg attcagtact ttctgcttat
    26941 gcgcaacaag ttggtgtttt gctcaccagc aagcatctaa ggttggttac tgctgaatca
    27001 tgcaccgggg gttggatagg gcaatccatt acggctattg cagggagttc aggctggtat
    27061 gaatgtggct ttatcacgta tagtaatcaa gcaaagcacc ggatgttggg cgttcaagcg
    27121 acaacattac ttaaatatgg cgctgtttca gaacaaactg cgcgagaaat ggtaacaggt
    27181 gctcttaaaa tgagcaccgc acaagtagcg atatcagtaa ctggtgttgc cggtccgagt
    27241 ggtggttcga aagaaaaacc cgtaggaatg gtatgtttcg cctgggaatt taaacaaagc
    27301 gcaattcata gcacgacaca ttttttttca ggtgaccgtg agtcagtcag gcggcaggcc
    27361 gtggctgccg ccttgcttgg tatcatcgag ttactcgaag gaatttcacc ttcacttgtt
    27421 tgaggcttcg ccatgactca acgtttgtga catgaggcga tccgtataag caatcgctac
    27481 agcagagatc agaaaagtga tatggatcga tgtctgagca atcaatgttt tctcatcata
    27541 agcatctgca ttgatgaatg ttttcagtag atgaatcgat gaaataccaa tgatcgcggt
    27601 tgcaagcttg atcttcagaa ccgatgcgtt aacatgcgac aaccattccg gttgatctgg
    27661 gtgcccctca agattcattc gtgatacaaa agtttcatag ccaccgatga tcaccatgat
    27721 cagcaaattg gaaatcatta cgacatcaat taaacccagc acgaccagca taattgcagt
    27781 ttcagtaatt tttagcggtg cttctgcctc ttcaggtgtt acgatatgta aaatatgttt
    27841 gagtgattct ttgtttccca taatggcacc aaccagatcc gttagctcaa cccaaaaatg
    27901 aaaaacataa acacattgcg ccagcacaag acccagataa agcggtagtt gtagccagcg
    27961 tgacagaaaa agaaaacgtg caaccgcaga aattcgtttg ttatcggttt tctgatttat
    28021 ttccataact ctcaagttca gtttttgaat taacaaagat aacccgtaaa gtatgaacct
    28081 tcacaggata attcaagcga tcaggccaag ccgcttgtac ttggcctaat tgtataaaga
    28141 cacgaaatta tagttttgct aaaacttcag caacggtttg ccccatcaaa gaaggggttg
    28201 gacaaatcgt gacgcctaat tcttttagtc gttcgacttt ctcagccgca ctctcaccag
    28261 cagaagagat aattgcgcca gcatgaccca tacggcggcc agtaggtgca gtaagtcctg
    28321 caatgtaagc caccaaaggc ttcgtcatat tctctttagc aaagataccc gcttcaactt
    28381 cttgcggtcc accaatttca ccgatcatga gcgccacttt ggtttcagag tcttgctcca
    28441 gtttctccag tacatcgcga tgggaactac caatgatcgg atctccacca atgccaacag
    28501 acgttgtaat accgattcct aatcggcgca tttggtcagc agcctcatag cccaatgttc
    28561 cggaacgacc aacaatgcca acattaccgc gcatatagat atgaccgggc atgatgccga
    28621 gcatggcgcg tccaggacta atcgttcccg cacaattggg gcctgtcatc atcatacgtt
    28681 gctctttcag gaagcggcgt agaaaacttt tgacggtcat catatcttgg gtcggaatac
    28741 catcagtaat ggaaacacaa tatttgattc cagcatcagc cgcttccata attgaatcgg
    28801 ctgcaaatgc gggtggtaca aaaacaatgc tagcttccgc gccaactttt tccacggctt
    28861 cttttaccgt gttaaatacc ggtaagccta aatgcgtttg ccccccttta ccgggcgtta
    28921 ctccgccaac aaccttagaa ccataatcga tcatctcctg tgcgtggaaa gtaccgatcc
    28981 taccagtaaa tccctgaacg ataattcgtg tattttcatt tattaaaatg gccactgtgt
    29041 gctcccctta actttgtttt gcaaccactt cattgcgcgc tttaaccact ttttctgcag
    29101 catcagccaa agtttccgcc gtaatgatcg gcaagccgct ttcatcaatg atgcgacaac
    29161 cttcttcgac attagtaccg gataaacgga caacgagtgg aattttcata tcgatgttct
    29221 taactgcttg cacaacccct tccgcaatcc agtcgcaacg attaatacct gcaaaaatat
    29281 taaccagcat ggctttcaca ccttggtccg ccagaacgag acggaatgct ttttcagttc
    29341 gttccgcaga ggcgccgcca cccacatcaa gaaaattagc cggctcgcca cctgccagtt
    29401 tgatcatatc catggtagcc atggctaaac ctgcgccgtt gatcatgcaa ccgatatcac
    29461 catctaaccc aacatagctc aggcccgctt cagcggctgc aacttcgcgc ggatcaattt
    29521 gggttttgtc acgtagctca gcaatttttt ggcggcgaaa tagtgcattt tcgtcaaaac
    29581 tcattttggc atccagcgcc atgagttcat tgcttttggt caccactaat ggattgattt
    29641 ccagaatatt ggcatccaaa tctcgcatag cacgataaca ccctttgatg gtttttaccg
    29701 catgattgag cagatcttca cccaacccca atgcaaaggc catggtacgt gcttggaaat
    29761 cttgcaggcc cactgccggc tcaatatgga ttttaatgat ggcatccgga tccgttactg
    29821 ctaaccgttc gatctccatt ccaccttgtg cagagccaac catgatgatg cgttcatgac
    29881 tgcgatcaat caaaaaagaa agataaagtt ctcttgcaat atcagttccc gcttcgatat
    29941 aaagtttaga acaaagcttg ccagcagcac ccgtttgatg tgtgaccaat ttctttccaa
    30001 gcaaggaccc tgcggcagcc tcaacctcag ggtgtgtttt acaaacttta ataccacccg
    30061 ctttgccacg tgcgccggaa tgaatttgtg ctttgacaac ccatacatta ccatcgattt
    30121 cgcgactacg ttgcacggct tcttcaacgg tatgtgcaag tcctccttcc gcaatcttaa
    30181 cgccatattg agccaagatt tctttggctt ggtactcatg aatatccaat gcctttcccc
    30241 ctattaattc aatcaatcat ataaaactta gtaatggcta tttatttttg gcagcaatgg
    30301 cttctgctgc tttgacgata ttattagcca tccgctcaga tgccgcatca atcaatcgac
    30361 catccaacga cgcagcgcct ttaccctgtg cagccgcttc tttcaatgcc gctaaaatcc
    30421 gcttggcttt ttcaacttca gcagatagcg gtgtaaatac ttcatttgct aatgcgatct
    30481 gagtcgggtg aatcgcccat ttaccttcac atcctaaggc tgctgctctt cgcgccgctt
    30541 gtttatagcc atctggatct tgaatatctc caaaaggccc atcaatcggt cgcaaaccat
    30601 aagcacggca cgctaccgtc atgcggctaa ttgctgcatg ccattgatcg ccaggataat
    30661 caggattgag tccaccaata ttggtcgttc tagcgcgatt acttgccgca taatcagcca
    30721 caccaaaatg cagcgcttcc aaacgaccag aagcaccatt gcgcgcaata tcttcaacat
    30781 tcgccatgcc cagcgctgtt tcaatcaaag cttcaatgcc gattctattg tttaagcgct
    30841 gctgcatttc cagttgattg agcatcgctt ccaccatata cacatcacca tagacaccga
    30901 ctttgggaat caaaatagtg tcgattttgt gcccagcttg ttcgactaaa tccacaacat
    30961 cacgcaccat gtattgtgta tccaaaccat tgatgcgcac tgatagcgtg ataccatggc
    31021 ctttccaatc aagatcattg atagcctgaa taatattttt tcttgcttga attttgtcat
    31081 ctggcgcaac ggcatcttcg agatccaaga aaataaaatc gaccccactt ttcagtgcct
    31141 tctcgaacat ttcaggacgt gaacccggca cagctagctc acaacgctgc acgcgctgaa
    31201 ttttagtttc atacaaggtg tgactcattt ttcctccagt aaaaaaatct tgttttaatg
    31261 cgccgaacta cgatgaaaaa aaggaaacaa aatcaactca aagctggatt aaccgtcaag
    31321 ctggcttcaa taggtgggcg ttatgtggtc cagaatttca aaataattgg gcactattta
    31381 tcatttaaat gcagcattat accacttgca cccgtggaat tttggtgaag gtaatcattg
    31441 cacggtcaag gatcgtgata aatttcacag ccagtaacca ttgttgtggt tcaataaagc
    31501 tgaatacctt gttaagagag ttaaactact aac
//
LOCUS       DAOQLJ010000008        29802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_496_length_29802_cov_6.749050, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000008 DAOQLJ010000000
VERSION     DAOQLJ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 29802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29802
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_496_length_29802_cov_6.749050"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1056)
                     /locus_tag="PK522_01280"
     CDS             complement(7..1056)
                     /locus_tag="PK522_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40396.1"
                     /translation="MIAKAKINITSVVGIREAIEQIFVLIRKHIDAYAQNTEDRMQIE
                     SCQYYVHQLNGMLEMLELEGVLFVSQKMEGLIEALLQEKVESPSQTRVVLKQATRAIY
                     RYLDALIDGISDNPATLLPIYRKLMQVQGIKEISESDLFFPDLRQDLPLPKTIPEGDP
                     SQKLSTVKKVRSEFQTGLLKWLKDTSNKEGLKQMFDAVNLAEQIPAPIEQRTFWWLSS
                     GFLDGLIHQDESIDLSTRRLCGKIEQEIRHFDKDAHVVANQLMREILYRVARSSSNSE
                     RIKEIENVFDWRASLSSFDQLANAVAGPDEDSIQPILDAMHEILLGANDQWREYSAGN
                     QESLEVLRQLFMRFK"
     gene            complement(1149..3149)
                     /locus_tag="PK522_01285"
     CDS             complement(1149..3149)
                     /locus_tag="PK522_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNC40397.1"
                     /translation="MAQNRFWLYGGIFLIFVFIALATMFVSLRYTKQSASYLEMIGRM
                     QIYNQNLTNASQQALLGNEIAFVQLQDSKNYLNQFSELLRGENKYPETLPTSMHVALS
                     DELLDEYQRVWRIKENKIGLLINSRDVLINVAGIANDINIASIRLQELLYELSVQMEQ
                     LGGLSKEVMITKLIGMLARSLTKSVNTLLSGEFVISDITEQLTKEREQLLVLLKALSD
                     GDSDLGIPALKDKRVLEKLVPIQALSKAIDDQVRIIQKQAPEVIAAKLALHDSFKINE
                     VLLSTLDKLKSKIEEQEASAKTWQDLVFYLSIGLVLGSLIIFGRTFSDYLQRQKAVGE
                     REVESAQKAILRLLDEMGALSEGNLTVRAAVTEDITGAIADSINYTVEELQKLVVQIN
                     QAGTNVNEVSSQAQRVSTQLLAATHGQSRQIEEATVSVLGMAESINTVASAAEEGANV
                     AKHSLTAAEKGAIAVRDAIMGMNEIRTSIQETAKRIKRLGESSQEIGEIIALISDITE
                     QTNVLALNAAIQAASAGEAGKGFSVIAQEVQRLAEHSAESTKQISSLVRAIQGDTKET
                     IAAMERSTYGVVEGTKRANATGEALEEIEEIANRLAQLVAKIFDATQIQTKSVDKVTK
                     NMENILAITRQTNDGTSQNAKLVKQISGFASELKASVAKFKV"
     gene            complement(3488..4999)
                     /gene="ilvA"
                     /locus_tag="PK522_01290"
     CDS             complement(3488..4999)
                     /gene="ilvA"
                     /locus_tag="PK522_01290"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HNC40398.1"
                     /translation="MRHDYLERILTARVYDVATETPLEKAANLSARIHNQVFLKREDT
                     QQVFSFKLRGAYNKMAKLSPTALKRGVIAASAGNHAQGVALAAQKLGSSATIVMPATT
                     PQIKINAVMAMGATVLLHGDSYNDAYEHAMTIAQQKETTFIHPYDDPDVIAGQGTIGM
                     EILRQHGAGIHAIFVPIGGGGLIAGIAAYVKRLYPNIKIVGVEPVDSDAMYRSLQKGR
                     RVNLSQVGLFADGVAVRHVGKETFRLCREFVDEVILVDSDSICAAIKDVFEDTRAILE
                     PSGALSVAGLKHYVAREKLLHRSLVAVASGANMNFDRLRYVSERAELGEQREAVLAVS
                     IPEEPGSFKKFCSMLGTRSITEFNYRYADSKQAHVFVGVSVQNREEVVKLIKSLEANG
                     LRTEDLSGNEMAKLHVRHMVGGRSKEVKHEILYRFEFPDRPGALMKFLNNMSHHWNIS
                     LFHYRNHGADFGRVLVGIQVPLSEKVDFQKFLDNLGYRYWNESHNPAYKLFLL"
     gene            complement(5337..7157)
                     /locus_tag="PK522_01295"
     CDS             complement(5337..7157)
                     /locus_tag="PK522_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HNC40399.1"
                     /translation="MLYRLNKAMQSMAYGIVSLFCLVLSTGSVLAKDNEVSFALTDNA
                     GRWFDTGTAIAGNRSIAVATPGVRVNFSGNSNTVHTRTSLIYPTGAAGMPFNTEPKKG
                     GDSITLTTPGLYVFTCSIHPYMFGAVIVDDPATSGLDLGASISLINGITVPTSSDLAT
                     RLLRTFFIATNPANWQNYASNAPWHITYPNVDVRVDIGVVNLPAVLNARYGNDISLPA
                     LSNPATQAVGEVWVATQFETTSGKSKPGTVTAVDGSSWQVTRKVALPSINMNNPHNMW
                     TDKDQNMIYVTQWFDKKLTVYNRQTGALIRNVSVGEAPAHVMTLTDNDRIHVTNNGDT
                     RTDSVMELSPLATNVLRRIDIGRGNPHAHWMSHDGKFMVTPNVLTGDTSQYNFRTNDV
                     EAILPVSGPLSHPLATGMMPDASKYYVANLLDSTITVVDMESHNVLKHINLIANYHPV
                     TGAITGPVGALPIQTPVSPNGKNMVTANTLTGTITILDTATDEIVAMLPCDPGCHGVQ
                     YGAKLGGGYYAYVTSKFSNRMLVVDPDPNNDGNPADAAIVGSIGLFATNRTLKDATIS
                     GNAGMGGQGILPIPVVYNGWVQKLPSNWSNLLTPAQRNPW"
     gene            complement(7235..8032)
                     /locus_tag="PK522_01300"
     CDS             complement(7235..8032)
                     /locus_tag="PK522_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40400.1"
                     /translation="MSSRDDDNSSGRSSDDGINHDINDDHGRGRGSDDGVNHDINDDH
                     GGRRNDDRSSDDNSSGGINVSGNHKAFKFEIVNGQVTAVYEVKDGELKSKSLTDGGRK
                     SYTVDGNDVIRTEIKPFGTEITRYSDDNGDGIYLRTSEQWQVSSNSNGVTPKFTDIIH
                     FSHTSGDDRIAVRGGEDCRGGNGADDFVVREAAHLHIGDFSSREGDLLIFDTGLGLTS
                     RDQLASYVTDTHHDGQNFIVNFGSDVSITLVGVQADQVSWNDVSVLS"
     gene            8923..10347
                     /locus_tag="PK522_01305"
     CDS             8923..10347
                     /locus_tag="PK522_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNC40401.1"
                     /translation="MSISCESFSNDQIRRGRQNTVIDDVDSRAMGLGGDDERLRPKAT
                     RHFQYGNTDISFTGAATLLDFSFENNFSFRENDAVDQFRFRPLVDLNTIFDFPKGYSL
                     FTEFRLRDSIKLQDDTQPSNFFELQVREFFLTAPILETIPIDLTLGRQQFFDLRRWYF
                     TDRMDAARLSAKSGPFDLFTAISTPVLVTNHSLQVFDNIAIKKEQMDILFNLDYALTE
                     RSFISGYLILGNKHQDRIEPGLLRNENPMWSGIRFIGDEKFKLASSESEFLSNLLKPR
                     IQYWLDAAFVSGSADSKKISGLGIDLGVTAIARKFQAKPYVTFGYAYGSGDSNPENDT
                     DHNFRQTGFQANSGRFGGVVNFDYYGVLYNPELRNMHILTAALGIRPTERSSIELVYH
                     RYAQDHAFARLPRDLRDGPITFSRNPDGIHSHLGDELDLVLGYRGINNVRLRSRTGYF
                     LPGAAYGEQRGSAFFTRLDVQFFF"
     gene            10412..11734
                     /locus_tag="PK522_01310"
     CDS             10412..11734
                     /locus_tag="PK522_01310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNC40402.1"
                     /translation="MKISVIGTGYVGLVTGACLAEMGNDVMCVDIDEKKIALLQEGKI
                     PIYEPGLESLVERNQAYGRLSFTTDIKKSTQHGLAQFIAVGTPPDEDGSADLQHVMAV
                     ARAIAEHMQEYKLIIDKSTVPVGTAKKVQAVITETLYRQGKVIDCSVVSNPEFLKEGN
                     AVEDFMKPERIVVGADDRKAIGLMKILYAPFNHNHNRLVLMDTASAELTKYAANAMLA
                     TRISFMNELALLAEKLGADIEHVRHGIGSDSRIGHHFLYAGCGYGGSCFPKDVQALQH
                     IAKEQDHELKILGAVELTNNRQKKVLLEKIVTKFNGNLNGKRIAIWGLAFKPNTDDMR
                     EAPSRILIEGLWSHNATVVAYDPAAMEQAKRIFGNNPRLQLADTPYSALENADALAIV
                     TEWKAFRTPDFSLIKNKLKQPIIFDGRNMYDPEAVRVEGIEYYSIGRN"
     gene            11746..12765
                     /locus_tag="PK522_01315"
     CDS             11746..12765
                     /locus_tag="PK522_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HNC40403.1"
                     /translation="MKTLVTGAAGFIGMHVTKKLLEDQFDVIGLDNLNDYYDIKLKED
                     RLKQLQAFKNFRFIALDIADKIAMEKIFNEEKFQLVINLAAQAGVRYSLKNPYAYINS
                     NIMGFMNILEGCRHHPVSHLVYASSSSVYGINTKMPFSVTDNVDHPASLYAATKKSNE
                     LMAHTYSHLFGIPTTGLRFFTVYGPWGRPDMSPSLFAHAIFQEKPIDVFNHGKMQRDF
                     TYIDDIVEGVIRILKKPATADPLYSRINPNPARSDAPYAIYNIGSHTPILLDDFIGEL
                     ETVIGKKAIKHLLPMQPGDVEATYASVADLKKAVGFEPKTSLSDGIKQFIDWFRKYYG
                     YTHQN"
     gene            12835..13740
                     /gene="galU"
                     /locus_tag="PK522_01320"
     CDS             12835..13740
                     /gene="galU"
                     /locus_tag="PK522_01320"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNC40404.1"
                     /translation="MKKITKAIFPVAGMGTRFLPATKASPKEMMPVIDKPLIQYAVEE
                     AVSAGITEMIFITGRNKRSIEDHFDKAYELESALELRGKQETLAELRSILPKHISCIY
                     IRQAEALGLGHAILCARPIICNEPFAVLLADDLIDAEKPVMQQMVEIYANNPYSILGI
                     QNVDPHKTNEYGIVECDLGPKNLHKITNMIEKPEPHNAPSTLGIVGRYILNPRIFRHL
                     EKITPGAIGEIQLTDAIASLRLEESVLAYQFAGTRHDCGSKLGYLKATIALALKHPEL
                     GKELSNYLSTQHALNLATHEDYTPA"
     gene            complement(13785..15332)
                     /locus_tag="PK522_01325"
     CDS             complement(13785..15332)
                     /locus_tag="PK522_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNC40405.1"
                     /translation="MRTDKSSHLSSGKRIALYPGAFRPPHAAHMHAVQYLANHPDIDE
                     VVIIISGRFRHIPGTDLALGPEVAEAIWQIYLEGLTNVRVVIASQTAVSYALDYFDKV
                     LPDQTLLFCIGEADLQSGDSRFYQIHQRAKETGISASIIMVPTGDLKIRSTALRASLV
                     DVENGSQIFMDCLPNQLNLAQQMQVWAICQNGWKTVIEVTRDRIQKVLSYSMQVRLAT
                     QLLPGKNRLDPVFFTQTEQGEHWVIKFAGSSLEVENNAQTDRPKSRNRLSVERLALKW
                     LNHHFRSGIKFPETLFWMKKEKMLIMTRLGFTGHSLAHRLSEGYFDRWVLNQVNAFLA
                     DNYHADSPAEAFWGDQSDDKYQWQHILEEHLKSARSHFFNNDFDQYLRQLQIKSLAAT
                     CHQLVHLDLQPKHILLNDSEIAIVDWESSSTFGDPAFDLGKLLGHFLFYAIQAQQTES
                     IVCLIEEVVLTFQLRTIGTCDHDFISRVYGFIGSTLLSLGQNIKESQFKQLTACVRKI
                     EFTYYSN"
     gene            complement(15399..16157)
                     /locus_tag="PK522_01330"
     CDS             complement(15399..16157)
                     /locus_tag="PK522_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40406.1"
                     /translation="MNQTWLLPLQQALERLDRSVTFFYRDDDAGWDTPALLRLLDLFD
                     DLQLPIDLAVIPKAITPELAAILNTRLVQYHNLIGLHQHGYAHVNHQTVGRKCEFGEM
                     RQFDQQLHDIVAGKEILHDYFGNRIDPIFTPPWNRCSQTTLDVLNTLPFTVLSRAEKA
                     TPFSLNQMVEISIHIDWLKRTHGERWSREQVIRLIAEKILMNSSIGIMLHHQEMDQED
                     LIAFKQFARLIERHPNIRHKRMRDLTDQIESAMR"
     gene            complement(16154..17329)
                     /locus_tag="PK522_01335"
     CDS             complement(16154..17329)
                     /locus_tag="PK522_01335"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC40407.1"
                     /translation="MKKPRLLFYCQHSMGMGHLVRSFRLAEVLVDAFDVVFLNGGPIP
                     SGISPPVDITLINLPALGMSEQDHLLFSHDTRYSVDEAKSIRQKMVLQAFDHYQPEVI
                     LIELFPFGRKKFADELIPLLSNAHQKNPKPLVLSSLRDILVQKRADQHRFENQVSQTL
                     NRYFHAVLLHCDPAFATLSEYFKPTIALQVPVFNTGFVAPPVADPGYSHNPGIIVSAG
                     GGIVGGELYRTAIHAQKLIWKEIAIPMTLVAGPFLPEEEWQHLVRIGHDVGGLTLHRT
                     VSGLMSLLSPCNISVSQCGYNTAMELLQSGVKALVVPFCTAHEDEQMSRAQKLMSAGA
                     VKLLHPALMTDKTLAGAILELWQAEKFQSKPLNTQGALNTLEIILRLRRTEYGGEVQ"
     gene            complement(17360..19111)
                     /locus_tag="PK522_01340"
     CDS             complement(17360..19111)
                     /locus_tag="PK522_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC40408.1"
                     /translation="MKKNDHAQSSLKRIFQIFLPMLWEYRKALFIAYLWSFIAVAAII
                     LSPWPLKFVIDYVLIDKALPDWLTHLTLGLSPVAQVLLLALVLALISAIGAFASATEK
                     NRNAKIREQLDLDVRERLLNHIQSVPAILFDNQKSGELVLRLVDDGQKVVRLLTKTTP
                     VIFRYLTTTLFAFVAMCMVSPFLGILGGLIVLMLSILVRRYAGPLQEASRFKRKTEGD
                     VSALAQEIIKGLANTQAHGMEHEVSQRFQSKTRISKRAGVEETRVAVLMELSMQLANG
                     LAVSLVIGGGGYLVLQDQLSLGSLTVCLAYMTQLLKPVEKINELAATVSRALVRGEYL
                     IHLFDQFSPIPDRPNATHLLQPKGIIRLEDVGYAYSSRDNNQSQPVLQHVNLVLEPNK
                     LVVLVGPSGSGKSTLLKLLLRMMDPTEGTLLFDGVPYQNIQLKHLRRQFAVMMQRNHV
                     FSGTLRTHFFIPGQDIDDQKIWNALELVAMADYVKQLPQELDSPLKEDGLNLSGGQRA
                     RLDLARTFLLDRPVLLLDEPLANVDPESSQIIMSALDRIRVGRSCLVATHQMEMAKRA
                     DQIVQLKNGKLLVGGFA"
     gene            complement(19108..20520)
                     /locus_tag="PK522_01345"
     CDS             complement(19108..20520)
                     /locus_tag="PK522_01345"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNC40409.1"
                     /translation="MAMVGHENKELPASDYYDACLLHMASLLDADLMADVLKAQLFPF
                     QPETFVVVSCKLVRVKYKPLKNCLAVYDFTVEHILTGEKRHFYITCRTYEFQGSRRRF
                     DRAVRQHDRNPSDFPAIFHVPELEVIGWVFPCESKLLSLPFISHAHYLKHVVLPRLIE
                     SLCGKDWQIVKFSSTVMHYVPEHTCTIRVELSLTRQGQKLAKTVFGKCYYNEAGEQTF
                     SIMRKLHESSLNGITPLRFPAPLFYDAELKLLWQEGLSGQTLRDATAGVESRRELLPQ
                     AARAIALFHRIPVDHQLSVVKITDMLTTLVNRMEVLSHVTPLDRRKLNQVILSLHRTV
                     PILAPTEIVTLHGDVHPQNLFLQNQSVAFIDLDSVVAGPAYHDVASWLCGMLYWGAMQ
                     HRSLQTLLPELGFFVTNYNQYAPFRVDEQDLSWFIAMALINERIFRCITRFKSGRLQI
                     INELLDWAEQFACGQQRIAI"
     gene            complement(20523..22439)
                     /locus_tag="PK522_01350"
     CDS             complement(20523..22439)
                     /locus_tag="PK522_01350"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNC40410.1"
                     /translation="MNSPSKPTLIWDTSYDALSPAQRLNYFSERQHIHFFPVLDREMT
                     EEAFVDDVLDNRFNFNGEVFLLTDPIDWLCNPSPDIEWHILLHKFYYAPGLGKAWVET
                     QDRRYLEKWVELITSWMEQTPIDFIAPDVTGRRIQNWIYSWYYFVASNRTERIDPQFH
                     DRFLDSIQKQVNYLCTHLAPARNHRTIALYSIFLASIVFPETTDAKHWQDFSLREIYQ
                     NVLQDLLPDGVQCELSTDYHHLVLKNYLAIRLLAKLNQITIQPEFDERLNRALDFAMF
                     AHKPDGEVPSFSDGDVRSFEDCLLQGASLYQREDLLFVGTRGQQGIAPSQRNAGFDVS
                     GYYILRSGWGQENTSYQDERYLMIDCGPLGEGNHGHFDALSFEAAAFGRSLIVDPARY
                     TYHEAKEVNWRVKFRSTESHNTVLVDGKNQTRYLAGLKRYKIKGPQPVTQLHAFQTQR
                     RVDYFHGSTRSSEYDALHHRQIAFIQGEYWIIVDSLYSASWHEYEQLFHLSTHAFQSV
                     QTIQDENCCQVITPNLVIATPANQNHQLIVETGYVSRRYGEKKEAPVLRFRKRAKNTR
                     FVSVLYPFKDGNPDIYVSIVEMNAGNHFEVNCLVYQKSGLGFADSCQFPKPELVTGEH
                     FSWDRKRIHSGDRI"
     gene            complement(22432..23766)
                     /locus_tag="PK522_01355"
     CDS             complement(22432..23766)
                     /locus_tag="PK522_01355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC40411.1"
                     /translation="MPQTNQAQIAYILKGFPRLTETFISNEIYLLEQLGTKLVLFSIK
                     PGELNKQHEVIQKIKSPLTYLPSVTSLSGSYLLIWVLRNIRPYLKHHANMVWVSPGRY
                     FATFRKACALAWQHRNGFLRLRKVFIKEFIQAGFIANQIQSRPNILLLHAHFCHGATT
                     VAWFVSQMTGLPFSFTAHAKDIYQKQLNPGRLLEQKLAAAQFVTTCTKTNHDYLTALH
                     DENCPIHAVYHGINTKKFIPRTNYQLNVGQPLILAVGRHVEKKGFVYLIEACAKLHLA
                     NIDFQCLIIGESGDQTDMIKTTIQNKKLESQVVLRNAVTQEELRELYQQATIFALPCV
                     ITKDGDRDGIPNVMAEAMATGLPIVSTPISGIPELVEDQVNGLLVPPRDVEGLAGALK
                     LLLADEMLRHKLGKAARQTICEQFDATVTIRSLQQLFQQALTYSKQHKPDNE"
     gene            complement(23753..25084)
                     /locus_tag="PK522_01360"
     CDS             complement(23753..25084)
                     /locus_tag="PK522_01360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC40412.1"
                     /translation="MSDRSNQVDVIHPNRVTDDSVRVSTRVGILVRCYPKLSETFILG
                     EILGLEKENIPLHIFSLFPPSDQLVNPAVNFVKASVTTLANGDSAMSMHIIKKHLQFF
                     VSHPKAYLSMLARILFSNEYGKIKDFWFAGKLATELISQKITHLHVHFISTPAAIGRI
                     ASDLCNITYSISAHAKDIYLSRADELAKKLHTAAFTVTCTQYNWVYLRSKTNYPEKIH
                     CMYHGINLAPFNQVLAASNDQPTDLPLILSVGRLREKKGFSTLIAACKKLSDLGQPFE
                     CIIAGYGPEQDRLQLQIDTLSLQNQVKLVGKKSHPEIIQLYSRARVFTLPCRVASDGD
                     RDGIPNVMLEAMAMGLPVVSTIVSGIPEVITHGENGLLLEPDDVTGLAAALLKLIRDP
                     LTGKAFGMKGRALIEEKFSTSRNLEILIGLLKQADQTLQKSSNKVNANATN"
     gene            complement(25081..26424)
                     /locus_tag="PK522_01365"
     CDS             complement(25081..26424)
                     /locus_tag="PK522_01365"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC40413.1"
                     /translation="MKRIMIYSHDTFGLGNIRRMLSIAEFIAAGREDYSILLITGSPM
                     LHAFRISQKIDYIKLPSLQRDIEGNYAVRSLALSMDEIIAIRANIILTTVEDYQPDIL
                     IVDKKPLGICAELQPAMEYLNRGENARHVRRLLLLRDILDSSEVTREIWKKRGYYDAI
                     EQYYDRVLVVGQSEIFDLTKEYAFPPAAQKKLEYCGYIRREAGLKGRQVVRSELGLSD
                     QHKLVFVTPGGGKDGFRLITNYLMGIDRYKNDSVYSLVLCGPEMLEEHVVTIKALAHG
                     KSRCLIQSFTDDLMSYMAAADVVVSMFGYNTACEILTLGKPAVVVPRIHPVREQWIRA
                     ERLAELGLVKIIHPELLESESLYQAIDSLLFQGERLRIPEQQINLNALPVIKQIIADE
                     IDIQNQLVNLSTGVTKTLSCLDRQPMEFDQQLAGQSKSLTPDLSLQSSQIEEKEL"
     gene            26728..27618
                     /locus_tag="PK522_01370"
     CDS             26728..27618
                     /locus_tag="PK522_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HNC40414.1"
                     /translation="MSTIPPTLVSALKQVLRPLIKLMLAKGLTFTYLIELLKEIFVEI
                     ADKDYKINNKPSTDSHLSLLTGIHRKDIKRLRNYSQTVEEAIPPNVSLGSRLVSIWTS
                     ETRFLDENNQPKPLPRFIKEGGEISFEGLVASVSKDIRSRVVLDEWLRIGIVHFNDEK
                     CVCLNTAAFVPSQGFDEKAYYFGESLHDHAAAATSNLLGGAKPYLERSVYYDALSTSS
                     IQQLAEQSEKLGMETLLAINKQAMALEQEDILEKNSKYRMRFGIYFYSEPVTPPISNN
                     ELDQPLPRSLVEAENNITQS"
     gene            27615..29411
                     /locus_tag="PK522_01375"
     CDS             27615..29411
                     /locus_tag="PK522_01375"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNC40415.1"
                     /translation="MKPWLHFLLHTVTLVITILLAVPNVFAKNNCETSLTQVNPAAGI
                     GGTGSKVAESGGGGTGIYAGGGIGGTGHPEKGIGGTGSKLAEDGGIGGTGIIGTITGF
                     ASVCVNGVEVHYDNNTPVTVDGRLSNARELAVGQVVITRAIGSGQEVTAKHIAVVHAA
                     VGPISHINPLTREMHVLGQVVQVTESRDLSNFKPGDWAQISGHRLANGAIIASRIEAA
                     TTATDAKINGHITHVDAKGFEVNGARIQHDAKSFPTKVSVGMEIAVAGHWNGNHLQAQ
                     HMQIEPTRQAIGQVSHLVIEGYVHAVRGKEINIANQPITLDSNAKVTGDNKEDLKLNQ
                     RVQVSGRVGPDQRIIADRVELKPESPTSLPERIDRNQTENDNRNKKSDSTNELGPRPN
                     NKSDEDKSNQNRSGESDRGKENSKRESSDHSGKQEAASDNKSRSSESLSSGKDGRDQT
                     DRQSQSTRSEDSERDQKNLSSDHDSRKSDSTSSSDTIAKDKLEKPIEEKPSNSSEKTD
                     SKLRLDHPSDLSKQDREQSESKTIDADSQMVPERMPMEKSQISDQPEKSTISDRDSLD
                     RPERPRDSDDLTERPSTDLNNIRDVMPDRIRD"
     gene            29431..>29802
                     /locus_tag="PK522_01380"
     CDS             29431..>29802
                     /locus_tag="PK522_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40416.1"
                     /translation="MSQTITRFSNRSIWTQTAVALCFLWNCSPISANGLSLSGGMDFT
                     TGNYGGSSATDIWYAPFTARYDYKKFSFRTTIPYLNLTGPGNVLGPGIGGVTIGDGGI
                     VPGVGGGSIGGGSGGVFVCNEE"
BASE COUNT         8764 a         6745 c         5901 g         8392 t
ORIGIN      
        1 tctcggttat ttgaatcgca tgaagagctg gcgcagtact tccaagcttt cttgattccc
       61 agcactatat tcccgccatt gatcatttgc tccaagcaaa atttcatgca tggcatcgag
      121 aattggttga attgaatctt catcgggccc tgccactgca ttagccagtt gatcaaagga
      181 ggacagagat gcccgccagt cgaaaacatt ctcaatttct ttaatacgtt cgctattgga
      241 agagctgcgc gctacgcgat aaagaatttc acgcattagt tgattcgcaa ccacatgcgc
      301 atctttatca aaatgacgaa tctcttgctc aatttttccg cataatcgac gtgtcgagag
      361 atcgatcgat tcatcttgat gtatcaatcc atccaagaat cctgaagaca accaccaaaa
      421 agttctctgt tcgatagggg caggaatttg ctccgccaaa ttgacggcat cgaacatttg
      481 ctttagtccc tctttattag aggtatcttt aagccatttg agcaatcccg tctgaaactc
      541 actccgtact tttttaaccg ttgataactt ctgagatgga tctccttcag gtatcgtttt
      601 aggtaaggga aggtcttgac gaaggtcagg aaaaaataaa tcgctttcag aaatttcttt
      661 gatgccctgt acttgcatca atttccgata aataggcaat aatgttgcag gattatcact
      721 gatgccatca atgagtgcgt ccaaataacg ataaatggct ctagtagctt gtttgagtac
      781 aacccttgtt tgtgagggag actctacttt ttcttgcagc aatgcttcaa tgagtccctc
      841 catcttttgg ctcacgaata aaaccccttc aagctcaagc atctcaagca tgccattgag
      901 ctggtgaaca taatactgac aagactcaat ttgcattcta tcttcagtat tctgcgcata
      961 ggcatcaata tgtttacgaa ttaacacaaa aatttgttca atcgcttctc tgatccctac
     1021 aacagaagtt atgttaattt tagctttagc aatcattcaa ttacccttca ttaatcaaat
     1081 gcgtgatacg aatccttcga ttccttactt tggtttctaa cttaatcaca atccaatcaa
     1141 aatgatcgtt aaaccttaaa tttggcaacc gacgctttta gctccgatgc aaatccagaa
     1201 atctgtttaa ccagttttgc gttttgtgaa gtgccatcgt ttgtttgccg agtaatcgcc
     1261 aaaatgtttt ccatattttt agtgacttta tctaccgact tagtttgtat ttgcgttgca
     1321 tcaaatattt tcgcgaccag ctgcgctaga cgattagcaa tttcttcaat ttcttccaac
     1381 gcttcacccg tggcattagc acgttttgta ccttcgacaa caccgtaagt acttctctcc
     1441 atcgctgcaa tcgtttcctt tgtatccccc tggatcgccc gcacaagtga actgatctgt
     1501 ttggtcgatt cagcagaatg ttcagcaagc cgctgaactt cttgtgcaat tacagaaaat
     1561 cctttccctg cctcacctgc agacgcagct tgaatggctg catttaatgc taaaacattg
     1621 gtttgctctg taatatctga aattaaggca ataatttcac cgatttcttg tgaactttca
     1681 cccaatcgtt tgatacgctt tgcagtctct tgaatggaag tacgtatttc attcattccc
     1741 ataatggcat ctctaacagc gattgcacct ttctcagctg cagttagcga atgtttcgcg
     1801 acattggcgc cttcttctgc agcactcgca accgtattga tcgattccgc catacccaaa
     1861 acagaaaccg ttgcctcctc gatttgccgt gactgaccgt gtgttgcagc taacagttgc
     1921 gttgaaactc gttgggcttg ggatgaaacc tcattaacat ttgtacccgc ttgattgatt
     1981 tgcacgacta atttttgcag ctcctcaacc gtataattaa tcgaatcagc aatggcaccc
     2041 gtgatatctt ctgtaactgc agcccgcacc gttaaattgc cttccgaaag tgcgcccatc
     2101 tcatctagca accttagaat tgctttttgt gctgactcga cctcgcgctc acctactgcc
     2161 ttttgccgct gcagataatc tgaaaatgtc cggccaaaaa taataagtga tcccaaaacc
     2221 agtccaatcg aaaggtaaaa aaccaaatcc tgccacgtct tagcagaagc ttcttgctct
     2281 tcaatcttac tttttaactt atcaagcgta ctcagtaaaa cttcattgat tttaaaagaa
     2341 tcgtgcaaag caagttttgc agcaatgact tcaggggctt gtttctgtat tatccggact
     2401 tgatcatcaa tcgcttttga aagtgcctgg attggtacca atttttctaa cacgcgttta
     2461 tctttaagcg caggaatacc caaatcgcta tcgccatcag atagcgcctt gagaagtacc
     2521 aatagttgtt cacgctcttt tgttaattgc tcagttatat ctgaaattac aaattcacct
     2581 gacaacaatg tattaacact ttttgtaaga cttctagcaa gcataccgat tagcttggtt
     2641 atcataacct ccttcgataa tccaccaagc tgctccattt gcaccgataa ttcgtagagc
     2701 aattcctgca gtcggatact cgctatattg atatcattcg ctattccggc aacattaata
     2761 agtacatccc ggctattaat cagtaggcca attttatttt ccttgatacg ccacacacgc
     2821 tgatattcat cgagtaattc atcagataat gcaacatgca tcgaggtagg gagggtttca
     2881 gggtatttat tctcgcctct taaaagctcc gagaactgat tcaaataatt tttactatct
     2941 tgaagttgga caaaagcaat ctcattgcct aataaagctt gctgagaagc atttgtcaaa
     3001 ttctgattat agatttgcat gcggcctatc atttcaagat aagatgcact ttgtttggtg
     3061 tatcggagac tcacgaacat ggttgcaagc gcaatgaaga caaagatcag aaatatgcct
     3121 ccatataacc agaatcgatt ttgggccatc cgatttttct gtgaaagatt tattttttgt
     3181 cccctacttc tatttgtagt ttgatttttt ctgatcaatt caggtgttaa ctttagtctg
     3241 gctagtatcc cttccatttc tttttttctc gattgtttga ataacagaaa ctcagaccat
     3301 caattgggat tattctcaat tcaagcagaa tatctgtcct acaaaaaaac caaaaattaa
     3361 ataaagccta aaagtatgtc cttttcttaa aatccaaaaa cagccatgtc actcgaagta
     3421 ggatttatta ttatcgtttg accctagttt cttctctcct catacttcct gtttaacgcc
     3481 accctttcta caacagaaac aacttgtatg ccggattatg gctctcattc cagtagcgat
     3541 aacctaagtt atcaagaaac ttctggaaat cgactttctc actgagtggc acttggatac
     3601 caactaatac gcgaccaaaa tcagcaccgt gattacggta gtgaaacaag ctaatattcc
     3661 agtggtggct catgttattt aaaaatttca tcaacgcacc tggcctatct ggaaactcaa
     3721 aacgatagag gatttcatgc tttacttctt tagagcgccc tcccaccatg tgtcgaacat
     3781 gcaattttgc catttcattg ccgcttaaat cttcggtacg aagtccgttc gcttccagac
     3841 ttttgatcaa cttaacgact tcttcacgat tctgaacgga cacgcccaca aagacatgtg
     3901 cttgcttaga atctgcatac cgatagttaa attcggtaat actgcgtgtt ccgagcatac
     3961 tgcaaaactt tttaaagcta ccgggttctt cgggaatcga aacagccagc acagcctcgc
     4021 gttgctcgcc cagctccgct ctttccgaaa catagcgtag tcgatcaaaa ttcatatttg
     4081 caccggatgc gacagcaacc aatgatctat gtagcaattt ctcacgtgca acatagtgtt
     4141 taagtcccgc aactgatagc gcaccagaag gttcaagaat cgcccgtgta tcttcgaaaa
     4201 catctttaat tgcagcgcaa atggaatcgg agtcaaccaa tatcacttca tcaacaaact
     4261 cacggcacaa tcgaaacgtc tccttgccaa catgcctcac tgccacgcca tctgcaaaca
     4321 aacccacctg ggaaaggttg acacgtcggc ctttttgtaa cgagcgatac atggcatccg
     4381 aatcaacggg ctcaacccca acaattttta tattaggata aagtcgtttc acgtaagcgg
     4441 cgattcccgc aatcaaccca ccaccgccaa tcggcacaaa aatggcatga atccctgcgc
     4501 catgttggcg cagaatttcc attccaatcg tgccctgtcc cgcaatcaca tcaggatcat
     4561 cataaggatg gatgaatgtc gtttctttct gctgcgcaat tgtcatggca tgctcgtaag
     4621 catcattata tgaatcacca tgaagcaata cggtagcacc catcgccatg actgcattga
     4681 ttttgatttg aggtgttgta gccggcatga caatagttgc cgacgaacct aatttctgcg
     4741 cagccagtgc taccccttgc gcatgatttc cagcggaagc ggcaataacc ccgcgcttaa
     4801 gagctgttgg tgagagtttt gccattttat tgtaggcacc cctcaactta aatgaaaata
     4861 cctgctgcgt atcttctctt ttcaagaaga cttgattgtg gatacgtgct gatagattgg
     4921 ccgctttttc taacggcgtt tcagttgcga catcatacac tcgggcagtc aggattcttt
     4981 cgagatagtc atgcctcata aaatgccagc ttatacaaag cttaataaat gttagattag
     5041 tctagtttta agtatagcag gattctatca tgctaaatga atcgtagatg taattcaacg
     5101 ttttgctatc gattaacatt ctgcacagct ttccagcata gtgtttagga agcataacta
     5161 cattcctgac tgatctatcg aaatcaattc ctagaaaaaa caaaaaccaa gctgaacgtc
     5221 catatcagct tggttttatt ttgtaactta taagttttta atctagccgc aaggttaaat
     5281 ggcagattca aatattaact gacagcataa tcttcgctaa cgtttaaaag tagtagttac
     5341 catggattgc gctgcgcagg tgttaacaga ttactccagt tagaaggtag tttttgtacc
     5401 caaccattgt acacaacggg aataggaaga atcccttgcc cacccattcc ggcattacca
     5461 gaaatggtgg catcttttaa cgtacgattc gtagcaaaaa gtccgatact ccccacaata
     5521 gccgcatctg ccggatttcc atcattatta ggatcagggt caaccactag catgcgattc
     5581 gagaatttac tggttacata tgcataataa ccgcccccga gcttagcacc atattgcaca
     5641 ccgtgacagc ctggatcaca gggcaacatc gctacgattt catctgttgc agtatcgaga
     5701 atggtaatcg tcccggtcaa tgtattagcg gtcaccatat ttttcccatt gggtgaaacc
     5761 ggagtttgga tcggtaaagc acctacgggc cctgttatcg cacccgtaac cggatggtag
     5821 tttgcgatta aattaatatg tttcaacaca ttgtgagatt ccatatcaac gaccgtgatc
     5881 gtgctatcga gtaagttggc cacataatat ttgctcgcat ccggcatcat accagtcgct
     5941 aaaggatggc ttagcggtcc actgactggc agaatcgctt cgacatcatt agtcctaaaa
     6001 ttatattgag atgtatctcc tgttaacaca ttaggcgtta ccatgaactt accgtcatgg
     6061 ctcatccaat gtgcgtgagg attgccacgc ccaatatcaa ttcggcgcaa cacattagtt
     6121 gctaacggag acaattccat cactgaatcg gttcgcgtat ccccattatt ggtgacatga
     6181 atccgatcat tatcggtcag tgtcataaca tgggcgggtg cctcgcccac tgaaacattc
     6241 ctgatcagcg caccagtttg gcggttataa acggtcaatt ttttatcaaa ccattgtgta
     6301 acgtagatca tattttgatc tttgtcagtc cacatattat gtggattatt catattgatc
     6361 gacggtaatg cgactttacg ggttacttgc caggaagaac catcgactgc ggtcacagtt
     6421 ccgggcttag atttcccaga tgtcgtttca aattgcgttg ccacccagac ttcaccgaca
     6481 gcttgcgtag caggattact taaggctggc agactaatat cattcccata gcgcgcattg
     6541 agtactgcag gtaggttcac aactccaata tcgacacgta catccacatt ggggtaagta
     6601 atatgccaag gcgcattgct tgcatagttt tgccagtttg ctggattggt tgcaataaag
     6661 aaagtacgca gtaatcgcgt tgccagatcg ctactggtcg gtactgtaat gccattgatc
     6721 aagctaatcg aagcgcctaa atcaagcccg cttgtggctg gatcatctac gatcactgca
     6781 ccaaacatat acgggtgaat gctgcacgtg aacacataga ggccaggggt tgtcaaagta
     6841 atactatccc cacccttctt aggctcggta ttaaatggca tgcccgctgc gccagtgggg
     6901 tagatcaaac tggtccttgt atgaaccgta tttgagtttc cagaaaaatt aacacggaca
     6961 ccgggtgttg caacggcaat tgaacgattt cccgcaattg cagtgccggt atcgaaccaa
     7021 cgcccagcat tgtcagttaa tgcaaaactg acttcgttat cctttgctag aacgcttcct
     7081 gtcgatagta ccaaacagaa caaacttact atgccataag ccatggattg catcgcttta
     7141 ttgagtcgat atagcatcaa aaatcccccc ttactatttt gatttactga ttgtgaagaa
     7201 attattatcc cctcacaatc gccatttacg atgtttaact taaaacactc acatcattcc
     7261 agctaacttg gtcagcctga acacctacta aggtaatgga cacatctgag ccaaaattaa
     7321 cgatgaaatt ttgaccatca tgatgcgtat cagtaacata gctcgctaac tgatctctgg
     7381 atgttaatcc taatcccgta tcaaaaataa gcaaatcacc ttcacgtgag ctaaaatcac
     7441 cgatatgtaa atgtgctgct tcacgcacaa cgaaatcatc cgctccattc ccaccgcgac
     7501 aatcttcccc acctctcacc gcaatacgat catcaccgct ggtatgcgaa aaatgaatga
     7561 tgtcagtaaa tttaggcgtg actccattgg aattagaaga aacctgccat tgctcagaag
     7621 tacgtaaata gattccgtcc ccattatcgt ctgaataccg ggtaatctct gttccaaatg
     7681 gtttaatttc agtacgaata acatcattac catcgacggt atagctcttt cttccaccat
     7741 ccgtcagtga tttagatttt aattccccgt ctttcacttc ataaactgcg gttacttggc
     7801 catttacaat ttcaaacttg aaagctttat gatttccaga aacattgata cccccagaag
     7861 agttgtcatc gctgcttctg tcatcattgc gccgtccgcc atgatcatcg ttaatatcat
     7921 ggttaacgcc atcatcacta ccacgtcctc taccatgatc gtcattgata tcatgattga
     7981 tcccatcatc actgctacga ccgctgctat tgtcatcatc acgtgagctc atgtttttct
     8041 cctaaagtta attaattaga aatgaagcat tattaaatta cctaccgaaa tgcatggaat
     8101 tttttcacgc atgagaaaaa ttatatgtgt gaaaatttta cacgtcaact atttttttat
     8161 tgatgtaata aaaatttact ccggcttata tgttcagttt tattttattt attagcgcta
     8221 gataaaactt gtgcaagctt acccaaagcg tcatacgcaa caagcgcatc gtaaacgctt
     8281 atttctatta ccgagcgtac tgcaacaata aatattattt gatgctattg gttaaaaaaa
     8341 gaactgtgaa cagcttctga ggagctcagg aagttattaa gaagcaagag ttattttgtt
     8401 ccttttattc aggttgaggt aattaccatt gatatacacc gaaggctagt gaaaaagtaa
     8461 acaagcacta tcttactcct tggacatggg ccataaagag cgcctaagga atcggatatt
     8521 tttatttatc gataacattc aattttttct accgaccaac aatcaggctt actagcgcct
     8581 gactctattt agtttagcgc ttccaaattt tctctcattt gatttgcagt tactcatcta
     8641 aatttatttg acacgggaaa ttaacccacg tatattaaat ttagatcgag ggaaataatc
     8701 actcgtttat gagtttcaaa ctcaataaaa agagccatgc ggtatgaaat ctatatttag
     8761 ccatttcaat taaattgcta ggaccttaca aatcgatctt cttacgaccg gacaaaatat
     8821 cgcccatgca aactcatgaa taccaaaaca ttaagaagat atatagaccg ttttcacata
     8881 tctgcgacat gtttggtttg tcaaattcta tgtataacta ttatgtcaat cagttgtgag
     8941 tctttctcta atgatcaaat caggcgggga aggcaaaaca ccgtcattga cgatgttgat
     9001 tcaagagcaa tggggcttgg aggagatgat gagcgattgc gacctaaagc aacaaggcac
     9061 tttcaatacg gaaatacaga cattagtttc acaggcgctg ccacactgct tgatttttct
     9121 ttcgaaaaca acttctcatt tcgggaaaat gatgcagtag atcaatttag atttagacca
     9181 ttagttgatc tcaatacaat ttttgatttt ccaaaaggct attctttatt cactgaattt
     9241 cgtctacggg atagcataaa actacaagac gatactcaac caagcaattt cttcgaactg
     9301 caagtacgtg agttttttct gacagcgccc attttggaaa ccatcccaat tgatttaact
     9361 ttaggtagac aacagttttt tgatttacgg cggtggtatt ttactgatcg aatggatgcc
     9421 gcacgtttat cagctaaaag tgggccattc gatttattta cagccatctc cacaccagtt
     9481 ttggttacca accactcatt acaagttttt gacaatattg caatcaagaa agaacagatg
     9541 gatattcttt tcaatcttga ttatgcctta acagaaagat ctttcataag cggatatcta
     9601 atcttaggta acaagcatca ggacagaatc gaacctgggt tgcttagaaa tgaaaatcca
     9661 atgtggagcg gcataagatt cattggtgat gaaaagttta agctcgcatc cagtgaatca
     9721 gaatttcttt caaatttatt aaaacccaga attcaatact ggttggatgc tgctttcgtt
     9781 tcaggtagcg ctgattcaaa aaagatcagc ggattaggaa ttgatcttgg tgttactgca
     9841 attgctcgaa aatttcaggc aaaaccttat gtcacctttg gctatgcgta cggatccggt
     9901 gatagcaatc cagaaaatga tactgatcat aatttccgcc agacaggctt tcaggccaat
     9961 agcggaagat ttggtggcgt tgtcaacttt gattactacg gagttttata taaccccgag
    10021 ctccggaata tgcacatttt gactgctgcc ctgggaattc gcccaaccga acgttcttct
    10081 atcgaactag tttatcaccg ctatgctcaa gaccatgctt ttgcaagatt accgcgagac
    10141 cttcgagatg ggcctatcac attcagtcga aatccggatg gtattcattc ccatctcggt
    10201 gacgaacttg atttggttct cggctatcgt ggcatcaaca atgttcggtt acgttccaga
    10261 actggctact ttttaccagg agcagcctac ggcgaacaga gaggatcagc attcttcaca
    10321 cgtttggatg tgcagttctt tttttagcgt atttcgatat ttaacaacct tcgtaattca
    10381 aagtaattaa cacaaacaat aagggacgaa aatgaaaatc agcgtgatcg gtacaggcta
    10441 tgttggatta gttacaggcg cttgcctagc tgagatgggc aatgatgtga tgtgtgttga
    10501 tatcgatgaa aaaaaaattg ccctgcttca agaaggaaaa atacctatct atgagccagg
    10561 acttgaaagc ctggtagaaa gaaatcaagc ttatgggcgt ctttccttca caaccgacat
    10621 aaaaaaaagc actcaacacg gtttagcgca atttatcgct gttgggacac cgcccgatga
    10681 agatggatca gctgatctac agcatgtcat ggctgtagca cgcgctattg cagaacacat
    10741 gcaagaatat aaattaatca tagacaaatc cactgtccct gtaggaaccg ctaaaaaggt
    10801 acaagcagtg attacggaaa cgctttatcg ccaaggaaaa gtaattgatt gcagtgttgt
    10861 ttcgaatccc gaatttttaa aagaagggaa tgcggtagaa gattttatga aaccagagcg
    10921 cattgtagtt ggcgcagatg atcgcaaagc cattggtctg atgaaaatcc tgtatgcacc
    10981 ctttaatcat aatcacaatc gcctagttct aatggataca gcatccgcag aattaaccaa
    11041 atatgctgcc aatgcaatgc tggcgacacg catatccttc atgaacgagt tagcattact
    11101 tgcagaaaaa ttaggtgcag atattgagca tgttcgtcat ggaatcggtt ctgattcacg
    11161 cattggtcat cattttcttt atgcgggttg tggttatggt ggatcctgct tccccaagga
    11221 tgtacaagca ttacaacata tcgctaaaga gcaagatcac gaattgaaaa ttcttggggc
    11281 agttgaatta accaacaacc gtcaaaaaaa ggttttgtta gaaaagatcg ttacaaaatt
    11341 taatggcaac ctaaatggta aaagaattgc aatttgggga ctcgcattta aaccgaatac
    11401 agatgatatg cgagaagcac caagtcgtat tttgatagaa gggttgtggt cacataacgc
    11461 gactgtagtt gcatatgatc ctgcggccat ggagcaagct aaacgaattt ttggtaacaa
    11521 tccacgttta caactcgccg atacaccgta ttcggcacta gaaaatgccg atgccctcgc
    11581 gattgttaca gaatggaaag cattccgtac accagatttc tcattgatca aaaacaaact
    11641 caaacaaccc atcattttcg atgggagaaa tatgtacgac ccagaggcgg tccgtgttga
    11701 aggaatcgaa tactattcga tcggtagaaa ttagccctta aaacgatgaa aacacttgtc
    11761 acgggtgctg ctggatttat cggtatgcat gtcaccaaaa aactcttaga agatcaattc
    11821 gatgtgatcg gtcttgataa tcttaatgat tattatgata tcaagctcaa ggaagatcgc
    11881 cttaagcaac tacaagcttt taaaaatttc cgcttcatcg cgcttgatat cgcagacaaa
    11941 atcgcaatgg agaaaatttt taatgaagaa aaatttcagc tagtcatcaa cctcgctgcc
    12001 caggctgggg ttcgttattc actcaagaat ccctatgctt atattaacag caatataatg
    12061 ggattcatga atatcctgga aggttgtcgc catcatccag tcagtcatct tgtgtatgcg
    12121 agcagctcca gtgtttatgg tattaacact aaaatgccat ttagcgtcac cgataacgtt
    12181 gatcatccag ctagcctgta tgctgccact aaaaaatcga atgaattaat ggctcacacc
    12241 tatagtcatt tatttggaat tcccactacg ggacttcgat tctttactgt ttatggccca
    12301 tggggtaggc cagatatgtc accaagtctg tttgctcatg caatttttca agagaagcct
    12361 attgatgtat tcaatcatgg gaaaatgcag cgcgatttta cttacatcga cgacattgta
    12421 gagggcgtaa tacgcatcct aaagaaacca gccacagctg atccgctata ttcccgaatc
    12481 aatcccaacc cagctcgtag cgacgctcct tatgcgatat ataacatcgg tagccataca
    12541 cctattctat tagatgattt tattggagaa ctcgaaacag tgatcggtaa aaaagcgatc
    12601 aagcatttat tacccatgca accaggagac gttgaagcta cctatgcttc tgtggcagat
    12661 ttaaaaaaag cagtcggttt tgagccgaaa acttcgctat ctgatggaat taagcaattc
    12721 atcgattggt tcaggaaata ttatgggtac acccatcaaa actagaatga ctagtcaaac
    12781 gagtaaatca aatgagtcat ttatctcaat cacccaaagc aggagaccgt catgatcaag
    12841 aaaataacta aagctatctt tccagttgca ggcatgggca cacgttttct accagcgact
    12901 aaagcgagtc ccaaggaaat gatgccggtt atcgataaac cattaattca atatgcggtt
    12961 gaagaagctg tatctgcggg aattaccgaa atgatcttca tcacaggacg aaataagcgt
    13021 tcaatcgaag atcactttga taaagcctat gaacttgagt cagcacttga gttaaggggt
    13081 aaacaggaaa ccctagccga attgcgcagt atcttgccca aacatattag ctgtatctat
    13141 atccgacaag cggaagcgtt aggactgggc catgccatct tatgtgcgcg cccgatcatt
    13201 tgtaatgaac catttgctgt tctacttgct gatgatctaa tcgatgcaga aaaaccagtt
    13261 atgcaacaaa tggtagaaat ttatgccaat aatccttact caattctcgg aatacaaaac
    13321 gtagatccac ataaaactaa tgaatatggc attgtagaat gtgatttggg tccgaaaaat
    13381 ctccataaaa ttaccaatat gattgagaaa cccgaacccc ataacgcgcc ttcaactctg
    13441 ggcattgttg ggcggtatat acttaacccc aggatcttca ggcacttgga gaagataacg
    13501 cctggcgcca taggagaaat ccaactaaca gatgcaatcg catctttacg cctagaagaa
    13561 agtgttcttg cttatcaatt tgcaggtacc cgccatgact gcggaagcaa attaggctac
    13621 ctcaaagcaa ccattgcgct tgcactcaaa cacccagaat tgggtaaaga gttgtcaaat
    13681 tatttgtcaa cccaacatgc tctaaatttg gctacccacg aagactatac ccctgcatga
    13741 tttgaaattt tcacttaaac cagcacaact cttctggaga ttttctaatt tgaatagtaa
    13801 gtaaattcga tttttctaac acacgctgtc aactgtttaa attggctttc ttttatattc
    13861 tgtcctaatg aaagcaatgt actcccaata aagccataga ctctcgaaat aaaatcatga
    13921 tcgcaagtac cgattgtgcg taactgaaac gtcaacacga cttcctctat cagacacacg
    13981 attgattctg tctgttgtgc ctgaattgca taaaacaaaa aatgaccgag tagtttcccc
    14041 aaatcaaagg caggatcgcc gaatgtactg ctcgattccc aatcaacaat cgcaatctca
    14101 ctatcgttca acaagatatg ctttggttga agatctaaat gcactagttg atgacacgtc
    14161 gctgcaagac tcttaatctg tagctgtctg agatactgat cgaaatcatt attaaaaaaa
    14221 tgacttcggg cgctttttag gtgctcttcc agaatgtgct gccattgata cttatcgtca
    14281 gactggtctc cccaaaatgc ctcagcagga gaatctgcgt gataattatc agcaagaaac
    14341 gcattgacct gatttaacac ccatcgatca aaataccctt cagacaatcg atgtgcaagt
    14401 gaatgacctg taaaccccaa tcgagtcatt atcagcattt tttctttctt catccaaaag
    14461 agcgtttctg gaaatttgat gcctgatcga aaatgatgat tcaaccattt cagcgctagc
    14521 ctctccacgc tcaatctatt gcgcgatttt ggtcgatcag tttgggcatt gttttctacc
    14581 tcaagcgaac tacctgcaaa cttaataacc caatgttcac cttgctctgt ttgggtaaag
    14641 aatactggat ccagtcgatt ctttcctgga agcaattgag tagccagcct tacttgcatg
    14701 gaataggata atactttctg tatacgatcg cgtgtaactt caatcacagt tttccagcca
    14761 ttttggcaaa tcgcccaaac ttgcatctgt tgagcaagat tcaattgatt aggtaggcaa
    14821 tccatgaata tctgactccc attctcaaca tctactagag atgcacgcaa ggctgtagaa
    14881 cgaatcttca aatctcccgt gggcaccatg atgatcgatg cagagatacc tgtttccttt
    14941 gctcgctgat ggatttgata aaatcgcgaa tcgccagatt gaaggtccgc ttctcctata
    15001 caaaaaagta aggtctgatc tggcaatacc ttatcaaaat aatcgagcgc ataactgaca
    15061 gcagtctggc tagcaattac gactcgcaca ttggttaacc cttcaagata aatctgccag
    15121 atcgcttctg ctacctcagg acctagcgct aaatcagtgc cgggtatatg ccggaacctc
    15181 ccagaaatga taattacgac ttcatcaata tctggatgat tcgccagata ttgaactgca
    15241 tgcatatgtg ctgcatgggg cggacgaaat gctcctggat acagagcaat ccgtttacct
    15301 gaagacaaat gcgatgattt atcggttctc atggattagt cgatcaaata gggatagcac
    15361 ggtgcccact ttcagcccga gaccaaaata caatctactc atctcattgc ggattctatc
    15421 tggtcagtca agtcacgcat ccgcttgtgc cgaatattgg gatgtcgctc tatcaaccga
    15481 gcaaattgct taaaggcgat taaatcttct tgatccattt cctggtgatg cagcatgatt
    15541 ccaatgcttg aattcattaa aattttttct gcgatcagtc ttataacttg ctcacgactc
    15601 caacgttccc catgagttcg ctttagccaa tcgatatgaa tcgatatttc aaccatctga
    15661 ttaagggaaa acggagtagc tttttctgct cgagacaaaa ctgtaaatgg taatgtattg
    15721 agcacatcca gggtcgtttg actacagcga ttccaaggtg gtgtaaaaat gggatcgatg
    15781 cgattaccaa aataatcatg caagatttct ttacctgcta cgatgtcatg cagctgctgg
    15841 tcaaattgcc gcatttcgcc aaattcacat tttctgccta ccgtttgatg attgacatgc
    15901 gcatagccat gttgatgtaa tccaataaga ttatggtatt gcacaagcct ggtattaagt
    15961 attgctgcca gctcaggagt aatcgcttta gggattaccg cgagatcaat aggcaactgc
    16021 agatcatcaa atagatcaag taatctcaat aatgccggag tgtcccaacc cgcatcatca
    16081 tcacgataaa agaaagtaac cgatcgatcc aatcgctcca gcgcttgttg tagtggaagc
    16141 agccatgtct gattcattgt acttcgcccc catattctgt tcgtctcagc ctgagaataa
    16201 tctcaagcgt atttagcgcc ccctgagtat tgaggggctt actctgaaat ttttccgctt
    16261 gccacaactc caagatagct ccagcaagtg ttttgtcggt catcagggcc gggtgaagca
    16321 gcttaacagc tcccgcgctc atcagcttct gcgcacgact catttgttca tcttcatggg
    16381 cggtacaaaa tggaaccact aatgctttca caccagattg gagtagctcc atcgccgtgt
    16441 tatagccaca ctggctgaca gaaatgttgc agggagataa caacgacatc aaccctgata
    16501 ccgttcggtg aagcgttagt ccaccaacat cgtgtccgat tctcacgaga tgctgccatt
    16561 cctcctcagg aagaaatggg ccagcaacca gcgtcattgg aattgcgatt tctttccaaa
    16621 ttagtttctg tgcatgaatt gcagttcgat ataactctcc tcctacaatg cctcctccgg
    16681 cagaaacaat gattccagga ttgtgcgagt aacccggatc agccacagga ggagcgacga
    16741 aacccgtatt aaaaacagga acctggagtg caatcgtagg cttgaagtat tcgcttaacg
    16801 tggcaaaagc agggtcgcaa tgaagcagaa ctgcatgaaa gtaacgattc aaagtttggc
    16861 tgacttggtt ttcaaatcga tgctggtcag cacgcttctg taccaagata tcacgcaggc
    16921 tagacaacac cagtggtttc ggattctttt gatgggcatt cgatagcaaa ggaatcaatt
    16981 cgtccgcaaa tttctttcgc ccaaaaggaa atagctcaat tagaattacc tccggttgat
    17041 aatgatcgaa cgcttgcaga accattttct gccgaatcga ttttgcctca tctacgctat
    17101 aacgcgtatc gtggctgaac aacaaatgat cctgctcaga cattcccaac gcgggaaggt
    17161 ttatcaatgt tatatcgaca ggggggctaa tacccgatgg aatcgggcct ccattgagaa
    17221 aaacaacatc aaaggcgtct accaatactt cagcaagtct gaaagaacga actaagtgtc
    17281 ccattcccat ggaatgctgg caataaaata gcagccttgg ttttttcaca ttaagtttct
    17341 taaattaaag aaagcagagt catgcgaaac caccaaccaa caatttgccg ttcttcagct
    17401 ggacaatttg atctgcacgt ttagccattt ccatttggtg cgttgcaaca agacaactgc
    17461 gaccgactcg aatacgatcc aacgcgctca taataatttg actggactct ggatcaacat
    17521 tggcaagcgg ttcatcgagc aataacacgg gcctgtcgag taagaaagta cgcgctaaat
    17581 cgagcctagc gcgctgccca cccgacagat taagcccatc ctctttaagc ggggagtcta
    17641 actcttgtgg tagctgctta acatagtccg ccatagcaac taactctagg gcattccaga
    17701 ttttctgatc gtcgatatcc tgccctggaa tgaaaaaatg ggttcgcaat gttccagaaa
    17761 aaacatgatt acgctgcatc atgacagcaa actgccgccg caaatgttta agctggatat
    17821 tctggtaagg aactccatcg aaaagcaaag taccttcagt cggatccatc atacgaagta
    17881 ataacttcag taatgtactt ttcccactac cactgggccc gaccaataca acgagtttat
    17941 tcggctctaa tacaaggttt acatgctgca aaacaggttg cgattggtta ttatctcgcg
    18001 atgaataggc ataaccaaca tcctctaggc gaattatccc tttaggctgc aagagatgag
    18061 tcgcattagg acgatctggt attggtgaaa attgatcaaa caaatgaatc aggtactctc
    18121 cacgaaccaa ggcacgagaa acagtggcag ccagctcatt gattttttcc accggcttta
    18181 ataattgtgt catgtaagcc aaacatactg tcaaactgcc aagtgaaagt tgatcctgga
    18241 gcaccaaata cccacctcct ccaatgacga gggaaactgc cagcccattg gccaactgca
    18301 tactgagttc catcaatacg gcaacacgtg tctcttcgac gccagcacgc ttactgatcc
    18361 gcgttttact ttgaaatcgc tgactgactt catgttccat accatgagct tgtgtatttg
    18421 ccagtccctt aattatctct tgggctaatg cagacacgtc tccttcagtc ttacgtttaa
    18481 aacgcgacgc ttcctgaagt ggccctgcat atcgtctgac caatatcgac aacattaaga
    18541 caatgagtcc ccctagtatt cctaggaatg gcgaaaccat gcacatcgcc acaaaagcaa
    18601 atagtgttgt cgtaaggtag cgaaaaatga cgggcgttgt tttagtcaat aatctgacaa
    18661 ctttctgacc atcgtctact aaccgaagaa cgagctcccc acttttctga ttatcaaaca
    18721 gaatggcagg aacgctttga atatgattga gtaatcgttc acgaacatct agatcaagtt
    18781 gttcacgtat ttttgcattt cgattctttt ctgttgcaga tgcaaaagca ccgatcgctg
    18841 atataagcgc gagcactaac gcaagtagca atacttgtgc aacaggagat aatcctagcg
    18901 ttaaatgagt taaccaatca ggtaacgcct tatcgatgag cacataatca ataacaaatt
    18961 ttagtggcca gggggataga ataatcgcgg caactgcaat gaaactccat aagtatgcga
    19021 tgaataaggc ttttctatac tcccataaca tgggtagaaa aatttgaaat attcttttca
    19081 aactagactg cgcgtgatca ttctttttca tattgcgatt cgctgctgac cacatgcaaa
    19141 ttgttcagcc cagtctagaa gttcattgat tatttgtaac cggccagatt taaaccgtgt
    19201 tatacaccga aaaatccgct cattaataag tgccatcgca ataaaccagc tcagatcctg
    19261 ctcatcaact cgaaatggtg catactggtt atagttcgtt acaaaaaaac ctaactctgg
    19321 cagcagcgtt tgcagagatc tatgctgcat cgcaccccag taaagcatcc cacaaagcca
    19381 gcttgcaacg tcatggtaag ctggcccagc aacaacgcta tcgagatcaa taaaagcaac
    19441 cgattggttc tgaagaaata aattctgtgg atgcacatcc ccatgcaaag taacaatctc
    19501 agtaggcgcc agtataggta ctgtgcggtg taacgataaa ataacctggt ttagtttgcg
    19561 tctatcgagt ggcgttacat gactcaaaac ttccatacga ttaaccaacg ttgtaagcat
    19621 gtcagtaatc ttgaccaccg aaagctgatg atctactgga atgcgatgaa ataatgcgat
    19681 tgcccttgcc gcttgtggca ataactcccg gcgtgactcc acaccagccg ttgcatcgcg
    19741 aagggtctgg ccacttaaac cttcttgcca aagtagcttc aactctgcat cataaaatag
    19801 tggtgcgggg aagcgaagtg gcgtgattcc gttgagggaa gattcgtgta gctttctcat
    19861 gatactgaaa gtttgctccc ctgcctcgtt gtagtaacat ttaccaaata ctgttttagc
    19921 aagcttctgg ccttgcctag tcaacgatag ctcaacacga atggtgcagg tatgctctgg
    19981 cacatagtgc attaccgtac ttgaaaattt aacgatctgc caatctttac cgcacaggga
    20041 ctcaatcagt cgcggtaaca ctacgtgctt caggtaatgt gcatggctta taaatggcaa
    20101 gctcaatagt ttcgattcgc aggggaaaac ccaaccaatg acttctagtt cagggacatg
    20161 aaatattgct ggaaaatcag atgggtttct atcatgctgc cttaccgctc tatcaaaacg
    20221 tcgcctagac ccttggaact cgtaggttct gcaggtgatg tagaaatgtc gtttctcccc
    20281 agtcaatata tgctcaacag taaaatcata aaccgcaaga caatttttaa gcggtttata
    20341 cttaacgcga acgagcttac atgagacaac cacaaatgtt tctggttgaa atggaaacaa
    20401 ttgcgctttc aacacatcgg ccattaaatc ggcatcaagc aatgaggcca tgtgcagaag
    20461 acaagcatca taataatcag aagcaggtag ttctttattc tcatgtccaa ccattgccat
    20521 aatcaaatcc ggtctcctga gtgtatgcgc tttctatccc agctaaaatg ctctccagta
    20581 accaattctg gtttagggaa ttgacaacta tcagcaaaac caagcccact tttttgataa
    20641 accaggcaat tcacttcaaa gtgattacct gcattcattt caacgatgga aacataaata
    20701 tccggattac cgtctttaaa gggatatagc actgatacaa accgcgtatt ttttgcgcgc
    20761 tttctaaatc ttagaacagg cgcttctttt ttctcaccat aacgcctaga aacatatcct
    20821 gtttctacaa ttaattgatg attctggttt gctggtgtag caatcaccaa attaggtgta
    20881 atgacttgac agcaattctc atcttgaatg gtttgaacag actggaatgc atgcgtggat
    20941 aagtgaaaga gttgttcata ttcgtgccaa gatgcgctgt acaagctatc aacaataatc
    21001 cagtattcac cttgaataaa tgcaatttgt ctatgatgca gtgcatcgta ctcagaactt
    21061 cgtgtcgaac catgaaaata atcaacacga cgctgagttt gaaatgcatg cagttgggta
    21121 actggttgcg gaccttttat cttgtatcgc ttcaaacccg caagatatcg ggtctgattc
    21181 ttgccatcga ctagcaccgt gttgtgcgac tctgtgctac gaaattttac ccgccagtta
    21241 acctcttttg cctcgtgata ggtatatcgt gcaggatcaa cgatcagtga gcgaccaaaa
    21301 gctgctgctt cgaaacttaa tgcatcaaag tgcccatgat tgccttcacc aaggggaccg
    21361 caatctatca ttaagtaacg ctcgtcctga taggaagtat tttcctgacc ccacccactt
    21421 ctaagaatat agtacccact gacatcaaaa ccagcattcc gttgagaagg cgctatccct
    21481 tgctgccccc gagtgccgac aaatagcaaa tcttctctct gatacaatga tgccccttgg
    21541 agcaagcaat cctcaaaact tctaacatca ccatcactaa aagaaggaac ctccccatcg
    21601 ggtttatgtg caaacatcgc aaaatccaaa gcacgattca agcgctcgtc aaactctggt
    21661 tggatagtaa tttgatttag ttttgccagc agtcgaatgg ctagatagtt tttcaacacc
    21721 aagtgatgat aatctgtcga caattcacat tgcacaccat caggtaataa gtcttgcaac
    21781 acattttgat atatttctcg cagcgaaaaa tcttgccaat gctttgcatc agtagtttca
    21841 gggaaaacaa tcgaagcaag aaaaattgaa tatagcgcaa tagttcggtg atttcgggct
    21901 ggagcgagat gagtgcacaa gtagttgacc tgtttttgga ttgaatcaag aaaccggtca
    21961 tgaaactgtg gatcaatccg ctcagtccga tttgaagcaa caaaatagta ccaactgtag
    22021 atccagtttt gaatgcgacg acctgttaca tcaggagcga taaaatcaat tggagtctgc
    22081 tccatccaac tggttattag ttcaacccat ttttctaaat atcttctatc ttgggtctca
    22141 acccatgctt tgccaagccc tggcgcataa taaaacttgt gtaatagaat atgccattct
    22201 atatctggac ttgggttgca cagccagtct attgggtcgg tcaataaaaa gacctctcca
    22261 ttaaaattga agcgattgtc tagaacatca tcaacaaagg cctcctcggt catttctcta
    22321 tccagcacag gaaaaaaatg gatatgctgc ctttcactaa aataatttaa acgctgcgcg
    22381 ggactcagtg catcatagga agtatcccag atcaaggtag gtttactggg gctattcatt
    22441 atccggctta tgctgttttg aataagttag tgcttgttga aatagttgct gcaacgagcg
    22501 aatggttaca gtcgcgtcaa actgctcaca aatcgtttgc cgtgccgctt ttcctagctt
    22561 atgccgcagc atttcatcag ctaacagtaa tttcagtgcg ccggctagtc cttccacatc
    22621 tctaggcgga acgagtaaac cgttgacttg gtcttccact agctctggaa tacccgatat
    22681 aggggtagaa acgattggca gtccagtggc catggcttcc gccatcacat tagggattcc
    22741 gtctcgatcc ccatctttag taatgacgca gggcaacgca aatatcgttg cttgctgata
    22801 aagttcccgg agttcctctt gagtcactgc gtttctgagc acgacttggg attctagttt
    22861 cttattctga attgtcgttt tgatcatgtc tgtttgatca ccggactctc cgataatcaa
    22921 acattgaaaa tcaatatttg ccagatgaag tttcgcgcac gcctcgatta aatagacaaa
    22981 tccttttttc tctacatgac gccctacagc taaaattagc ggctgcccca catttaattg
    23041 gtaattagtt cgtggaataa attttttggt attgatccca tggtacacag catgaatagg
    23101 gcaattctca tcatgcagcg ccgtcaaata gtcatgattg gtttttgtgc atgtcgtgac
    23161 aaattgagcg gctgctaatt tttgctctaa gagtcgcccg ggattgagct gcttctgata
    23221 aatatcctta gcatgggcag taaagctaaa aggtagtccc gtcatttggc ttacaaacca
    23281 agccactgtc gttgcgccat gacagaaatg cgcatgcaac aacaaaatat taggacgact
    23341 ttgaatttga ttggcgataa aaccagcttg gataaattct ttaataaata ctttgcgtaa
    23401 gcgtaagaaa ccattccggt gctgccatgc taaagcacaa gctttccgaa aagtagcaaa
    23461 ataacggcct ggactcaccc ataccatgtt tgcatggtgc tttaaataag gtcttatatt
    23521 tctaagtacc cagatcaaca aataactacc ggataatgat gtgactgaag gcaaataagt
    23581 caaaggagac ttaatcttct ggatgacctc atgttgtttg tttaactccc ccggtttgat
    23641 agaaaacaag acaagttttg tgcctaattg ctctagcaag tatatttcgt tcgaaataaa
    23701 ggtttcagta agcctaggga accccttcaa tatataggct atttgggctt gattagtttg
    23761 tggcattggc attcacctta ttactgcttt tctgtagcgt ttgatcagcc tgcttcaaca
    23821 aaccaatcag tatctccaga tttctacttg tagagaactt ttcctcgatc agcgctcttc
    23881 ctttcattcc aaacgccttg ccagtgagtg gatctcgtat cagcttcaat agtgcggcag
    23941 ctagcccagt aacatcatcc ggttcaagca ataagccatt ttctccgtgc gtaatcactt
    24001 ctgggatacc ggaaactatc gttgaaacca cgggtaaacc cattgccatt gcttctaaca
    24061 taacattggg aattccatca cgatcgccat ctgatgccac acggcatggt aatgtaaata
    24121 cccttgcccg actgtataac tgaataatct ctggatgtga ttttttcccg actaatttca
    24181 cctggttttg gagtgaaagc gtatcgattt gtagctgcaa cctatcttgt tcagggccat
    24241 aaccagcaat aatgcattca aaaggctgac ccaagtcgct taattttttg caagcagcga
    24301 ttaatgtgga aaatcctttt ttctcacgta aacgtcctac actcaatata agcggtaaat
    24361 ctgtaggctg atcattgctc gctgcaagta cctgattaaa aggcgctagg tttataccat
    24421 ggtacatgca atgaattttt tctggataat tcgtttttga tctaagatag acccagttat
    24481 attgggtaca tgtgaccgta aaggcggcag tatgcaattt ctttgctaac tcatcagcac
    24541 gagacaaata aatatctttg gcgtgcgccg aaatactata agtaatgttg caaaggtcgc
    24601 ttgcgatccg cccaatcgcg gcgggtgtag aaataaaatg tacgtgaaga tgtgtaattt
    24661 tttgactgat cagctcagta gccagtttcc ctgcaaacca aaaatctttg atcttcccat
    24721 attcattgct gaataaaata cgtgccagca ttgatagata agctttagga tgagatacaa
    24781 aaaattgcaa atgttttttg atgatatgca ttgacattgc actatcacca tttgccagtg
    24841 tcgttaccga tgctttgaca aaattgacag caggattaac tagctgatcg cttggcggaa
    24901 ataacgaaaa aatatggagt ggaatgtttt ctttttctag tcctaaaatt tctcccaaaa
    24961 taaatgtttc agaaagttta gggtaacacc taaccaatat gccaacacgt gtactcactc
    25021 gcactgaatc gtcagttaca cgattcggat gaatgacgtc gacctgattg gaacggtcac
    25081 tcataattct ttctcttcaa tttgcgatga ttgcagagat aaatcggggg tcaaggactt
    25141 ggattgtccc gctagctgct gatcaaattc cattggttgg cgatctaaac acgagagtgt
    25201 cttagttacc cctgtagaga ggttgacaag ctgattttga atatcaattt catcagcgat
    25261 gatctgcttg attacaggaa gcgcattcaa gtttatctgc tgctctggta tccgtaatcg
    25321 ttcaccctga aataacaatg aatcgatggc ttgataaaga ctctcacttt ctaaaagctc
    25381 cgggtgaatg attttaacca aacctaattc tgctaggcgc tcagcccgta tccactgttc
    25441 tctaacaggg tggattctag gcacgacgac agcaggtttt cctaacgtca aaatttcgca
    25501 cgccgtgtta tagccaaaca tcgatactac gacatcagct gccgccatat agctcattaa
    25561 gtcatcagtg aatgactgaa tcaaacatcg ggattttccg tgagcgagcg cttttatggt
    25621 tacaacatgc tcttcaagca tctctggacc acataacacc aatgaatata ccgaatcgtt
    25681 tttataacga tcgatcccca tcagatagtt ggtaattagt ctaaatccat cctttccacc
    25741 acctggcgtc acaaatacca acttatgctg atcgctgagg ccgagttcac tccgtactac
    25801 ttgccggcct ttgagtcccg cttcacgacg gatgtagcca caatactcaa gtttcttttg
    25861 tgctgctgga ggaaaggcat actctttggt caaatcgaag atttctgact ggccaacgac
    25921 cagaactcga tcataatatt gctcaatcgc atcgtaatat cctcttttct tccaaatctc
    25981 acgtgtgact tcagaactat caagaatatc gcgtagcaat aacaatcttc gaacatgccg
    26041 agcattctca cctcgattta agtattccat ggcaggctgt agttctgcgc aaattccgag
    26101 tggtttctta tcgacaatca aaatatctgg ctgataatcc tcgactgtgg tcaggataat
    26161 gttggcgcgt atggcaataa tctcatccat actcagtgcc aatgagcgga cagcatagtt
    26221 gccttcgata tcacgttgca gagaaggcaa tttaatgtaa tcgattttct gggaaatccg
    26281 gaaagcatgt agcatgggtg atccggttat gagcaaaata ctgtaatctt cgcgcccagc
    26341 cgcaatgaac tcagcaatcg ataacatgcg ccggatattg cccaacccga aagtgtcatg
    26401 cgaataaatc atgatgcgtt tcattattta tatccctact tttcagtttg ataacgttca
    26461 gtaccttaaa tttggtttca ggattccaag tcgcctttac ttaacgaaac agaattatta
    26521 tcgtaaatcc aattatatgt gtgaatattt ccctcgattt gatataatat acgcatggtc
    26581 gattcctgtg tcaacacatt gatgtaaata tttcccacac atatgataga attgctgcta
    26641 taactgattg gcaaggatcg aatatttatc tagcaacgaa ccgagataca aacttttccg
    26701 ataatcagaa ttacaataaa tataacaatg tcgactattc cacccacact tgtatccgca
    26761 ttaaaacaag tgctgcgtcc gctgattaag cttatgctag ccaaagggtt aacattcact
    26821 tacctaattg aattactaaa agaaattttt gtagaaatcg ctgacaagga ttataaaatc
    26881 aacaataagc cctcgacaga cagtcactta agtttactca cagggattca tcgtaaagac
    26941 atcaagcgac ttcgaaatta ctcccaaaca gtagaagaag ccattccgcc caatgtttcc
    27001 ttaggatctc gcttagtgag catttggacc agtgaaactc gtttcctaga tgaaaacaac
    27061 caacccaagc ctttaccccg atttatcaaa gaaggaggag aaatttcctt tgaagggcta
    27121 gttgcaagcg tcagtaaaga tattcgttca cgcgtagtgc tggatgaatg gctacgcatc
    27181 ggcatcgttc atttcaatga tgaaaaatgc gtttgcctga atactgctgc ttttgtaccg
    27241 agtcagggat ttgatgaaaa agcctattac ttcggagaaa gtctccatga ccacgcagca
    27301 gcagctacta gcaatctact cggcggtgcc aaaccttact tagaacgcag cgtttattat
    27361 gacgcactga gtacatcttc tattcaacaa ttagcggagc aatcagaaaa actcggaatg
    27421 gaaacgctgt tagcaatcaa taagcaagca atggccttgg aacaggaaga tattcttgaa
    27481 aagaattcta agtatcggat gcgctttggg atttatttct atagcgaacc ggttacaccg
    27541 cccatatcca ataatgaatt ggatcaacca cttccgcgca gcttagtgga agctgaaaat
    27601 aacataacgc aatcatgaaa ccctggcttc acttcctctt acatacagtc acgctcgtaa
    27661 tcacgattct attggctgtt ccgaatgtat ttgccaaaaa caattgtgaa acgagtctaa
    27721 ctcaggttaa tcccgctgct ggcattggtg gaactggttc aaaagtagct gaatcgggcg
    27781 gtggtggcac tggaatctat gcaggaggag gaatcggtgg tacaggtcat cctgagaaag
    27841 gaatcggtgg cacgggtagc aaacttgctg aagatggcgg tatcggtggc accggcatca
    27901 tcggaaccat cactggtttt gccagcgtct gcgtcaatgg tgttgaagtt cattacgata
    27961 ataatacacc tgtaactgtg gacggtcggc tttccaatgc ccgtgagctt gccgttggac
    28021 aggtggtcat cacccgtgca ataggttctg gacaagaagt cactgccaaa catattgcgg
    28081 tcgttcatgc ggcagttggc cccatcagcc atatcaatcc actgactaga gaaatgcatg
    28141 tgttgggaca agtcgtacaa gttactgaat cccgtgatct ttctaacttt aaaccaggcg
    28201 attgggctca aataagtgga caccgtttag ccaacggcgc aattattgca tcacggattg
    28261 aagcagcaac taccgcaacg gatgccaaaa tcaatggcca tatcacacat gtcgatgcca
    28321 aagggtttga ggtcaatggc gctcgaatac aacatgatgc gaagtctttc ccaacaaaag
    28381 tttcagtagg aatggaaatt gcggtagccg gacattggaa tggcaatcat ctgcaagcgc
    28441 agcatatgca aattgaacca acacgacaag ccattggcca ggtaagtcat ctcgtaatcg
    28501 agggctacgt ccatgccgta cgtggtaaag aaataaacat tgccaatcag ccgatcacac
    28561 ttgactccaa tgcgaaggta acaggtgata acaaagaaga tcttaagtta aatcaacgtg
    28621 tccaggtcag tggtcgagta ggtcccgatc agagaattat tgctgatcgg gtagaattaa
    28681 aaccggaatc accaacatcg ctaccagaaa gaatcgatcg gaatcaaacg gaaaacgata
    28741 atcggaacaa aaaatcggat tcgaccaatg aactggggcc tcgcccaaac aataaatccg
    28801 atgaagataa atctaaccag aatcgatctg gtgaatctga ccgcggaaaa gagaacagca
    28861 aacgtgaatc atccgatcat tcaggaaaac aggaggctgc ttctgataat aaatcccgtt
    28921 ctagcgaatc tctcagctct ggaaaagatg ggcgagatca gactgaccgt caatcacaat
    28981 caactcgatc tgaagattcc gaacgtgatc agaaaaactt gtcttcagat catgattcac
    29041 gaaaatcgga ttcaacgagt tcatcagata ctattgctaa agacaaattg gaaaagccca
    29101 ttgaagaaaa accatctaac tcgtcagaaa aaacagatag caagctaaga ttagatcatc
    29161 cctctgattt atcgaaacaa gaccgcgaac aatctgaatc aaaaaccata gacgctgatt
    29221 cacaaatggt tcctgagcga atgcctatgg agaaatcaca aatttcggat caaccagaaa
    29281 aatcaacgat ctcagatcga gattctctgg atcggcccga gcgccctagg gattctgatg
    29341 atctaactga acgcccaagt accgatttaa acaatatcag agatgtgatg cctgatagaa
    29401 ttcgagatta acccctgcta atttataact atgagtcaaa ccattacccg attttctaat
    29461 cgatcgattt ggacacaaac agccgttgca ctctgttttc tgtggaattg ctcgccaatc
    29521 tcggcaaatg gactcagctt gagtggcggt atggatttta ccaccggtaa ctacggcgga
    29581 tcatcggcaa cggacatctg gtatgcgcca tttacagcac gctacgatta taaaaaattc
    29641 tcattcagaa caaccatccc ttatctcaat ttaacgggcc caggcaatgt actcgggcct
    29701 ggtattggtg gagttacgat tggtgatggt gggatcgtcc ctggagtcgg tggtggaagc
    29761 atcggtggcg gaagtggggg ggtttttgtt tgtaatgaag aa
//
LOCUS       DAOQLJ010000009        29574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_501_length_29574_cov_7.292998, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000009 DAOQLJ010000000
VERSION     DAOQLJ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 29574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29574
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_501_length_29574_cov_7.292998"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..833
                     /locus_tag="PK522_01385"
     CDS             324..833
                     /locus_tag="PK522_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HNC40417.1"
                     /translation="MVRRTLTALAASLLLLPVSVLAAGYGPAGCGLGSMVIGSKPGME
                     QVFAATTNGTSGSQTFGITSGTSNCGDHGLVNLSKEREVFAQENYTSLVKEMAQGKGE
                     SLSTLASLYQCPATTHGEFGTLVQEKFDYLVANDQTTSAELLSKLDGQLMQHPNLANS
                     CNIKLSALN"
     gene            899..2812
                     /locus_tag="PK522_01390"
     CDS             899..2812
                     /locus_tag="PK522_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNC40418.1"
                     /translation="MSYRILSFGLVFCFFLFSHLALANNEEALRYLIELQQQAQQKKL
                     AQARVWHRLLKYNPQLLRGVMSEADGLDFFNAPQGKIDSDAELAATLASFFLPLSDVP
                     EGKEHPQCNFPARFKWLNQQLSFDPQRLDIQSCDRLDRWLGVLDPVGVTLVFASYFMN
                     NPASMFGHTLIRIDSRRTGPDNHLINYGANYAAIPDTDNAFLYALKGLIGSFQGRFAI
                     FPYYTKVQEYNNWESRDLWEYELQFNEDQLHMLLLHLWELGGTYFDYYYFQENCSYHV
                     LSLLEIANPDLFLKDSFFFSVIPTDTVKVVLAQEGLVKQVVYRPAVLNLMNDKRLRMT
                     SDERKLFEALIKGNITIENPSFTELPVRSQANLLNAYMDYRQYYSMQEDSNHEIKIPR
                     AVLLARSRLPPTEDNDQTIRFSSPPHQGHGTDRFRLGWGHNDREPFVEFAYRPAYHDF
                     MAKNVGYDKNSEIIFMDFKLRYYLESERVRLDQAKLLSITSFNPYDPLFARTSWRGEI
                     EIDTLRDRDCHYCNSFKGSYGRGMAYRPQFFSPLLLFSFIDVKTEFSPHLNQNYRLGG
                     DVEVGAYYDITDNLRVKLAGSYQVFFLGESKRFFTAQFLTRYAIAQDLDIRMELNRYD
                     HNHEGIFAINYFF"
     gene            complement(2796..3656)
                     /locus_tag="PK522_01395"
     CDS             complement(2796..3656)
                     /locus_tag="PK522_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC40419.1"
                     /translation="MKAFRLLMLLSTWLLTSCSSVLYYPSQNRLYYDPKSVGFSPTDI
                     YFTDAAQRKLHGWWFPSTITPAKATVIFFHGNAENLTSHFMHLAWITAEGYNLFIFDY
                     PGYGLSEGKPTPQSCVEAGHAALDWVLSHDPENLPMIVYGQSMGGIIALRTVIDKRED
                     INLKLVVAESTFDSFQQIARHKLSQHWFTWPLQPLSYLLLSDRWAPTHLETIAPVPVL
                     VIHGQQDNVVEPQFGEAIYEKLSEPKSIWRIPNGNHTDIFWQHEKKYRTVFLNFLTQL
                     DLNQSVRKNN"
     gene            complement(4011..4601)
                     /locus_tag="PK522_01400"
     CDS             complement(4011..4601)
                     /locus_tag="PK522_01400"
                     /inference="COORDINATES: protein motif:HMM:NF038127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DVUA0089 family protein"
                     /protein_id="HNC40420.1"
                     /translation="MKKMFLAVCIVFALSVAQVGAAPISSSFTGSFNTDDDVEFFSFA
                     VTEPSLVTLRTWSYAGGTNAEGNTISAGGFDPIITLFNGTGNLIDDNDDGAGVAADPV
                     TGAEFDAVLSIFLDIGNYTASLTQYANFAIGPNLADGFTGSGVTGFIDADGNTRTSAW
                     AFDIVISELPEPTSVALLGLGLVSLAFTRRSIKKIS"
     gene            5168..5608
                     /gene="msrB"
                     /locus_tag="PK522_01405"
     CDS             5168..5608
                     /gene="msrB"
                     /locus_tag="PK522_01405"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNC40421.1"
                     /translation="MINWSQILMLAKLGNPPPSQRVEKTEAEWKSLLTDEQYFVLRQK
                     GTEPPHSSKLCTQFEPGLYACAGCGTELFDASSKFNSGTGWPSFTQPVAENVVAYYLD
                     TSYGMQRIEVNCNICGGHLGHAFPDGPEPSGLRFCINALSLKKV"
     gene            5635..6645
                     /locus_tag="PK522_01410"
     CDS             5635..6645
                     /locus_tag="PK522_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HNC40422.1"
                     /translation="MTLQHNPLIASQLPIRLVIHGGAGAISRARFTKEREQTYQQTLR
                     EALLAGYAVLNAGGSSIEAVTEAIVVMEDSPLFNAGKGSVFTHEGHNELDAAIMDGAN
                     RMAGAVAGVKTIRNPIRAARAVMTKSNHVMLIAEGAEAFARDQRLEIVDATYFYTQQR
                     WEQLQRTIERERVQHQQAIEADSLLVEKDDKYGTVGAVALDRSGNLAAGTSTGGLVNK
                     RYGRVGDSPIIGAGTYADNRSVAVSGTGTGEMFIRTAAAHSIATRVQLKQLSITEAAD
                     QILAEIAAIGGDGGVIVLDKSGHYTMRFITSGMYRGTIGGDGVPWVGIFPEAEIALNA
                     KK"
     gene            complement(6691..8019)
                     /locus_tag="PK522_01415"
     CDS             complement(6691..8019)
                     /locus_tag="PK522_01415"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNC40423.1"
                     /translation="MPSIASMIKKPRISHYYQFILVGLVLLCLSVLTFTVHANSSAQV
                     TSTRVWAGPEYTRVTLESNLPIKYTLSLQSSPDQIVLDLENTGLNPPLGSLPTKIDAK
                     DPLIKKAHIEYLSSNGVRLTLALKTKAVPRTFTLDPMDKFGYRLVLDIFPPDRAATKP
                     NGHHDDPLMALVKASEEKIATKAKNANKPEITLASFNPRPEKQKTIIVAIDAGHGGKD
                     PGAIGPRGSQEKDITLSIAKKLKAKIDKEPGMHAVLTRTGDYFLPLAVRRTKARSANA
                     DLFISVHADAVPRNDARGSSVYTLSENGATSTTASWLAQKENESDRIGGIKLNTKDHY
                     LRKTLIDLSINATINDSIKLAGTVLSAIGNVNHLHKRQVEQAGFAVLKSPDIPSILVE
                     TAFISNPTEEEKLKSENYQDRMASAIVSGIKRYFSNSRAIPTRTELAKVN"
     gene            complement(7998..8495)
                     /gene="tsaE"
                     /locus_tag="PK522_01420"
     CDS             complement(7998..8495)
                     /gene="tsaE"
                     /locus_tag="PK522_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNC40424.1"
                     /translation="MHNRDCSEAVPNEEQQYCLHLIDEAATLAFGAKIATTLHAGLII
                     FLKGDLGAGKTALTRGILRGMGYQGKVKSPTYNLVEIYNFSRLYLYHFDFYRFNDYSE
                     WEAAGFRDYFNSETICLVEWPEKGGDLLPTADLTIKLQFANRGRFAGICANTTQGKKC
                     LALLA"
     gene            8490..9575
                     /gene="queG"
                     /locus_tag="PK522_01425"
     CDS             8490..9575
                     /gene="queG"
                     /locus_tag="PK522_01425"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNC40425.1"
                     /translation="MQEEISQNGTVDPVSLKQSITNWAHQLGFQDIRIANAEADMSSV
                     ETGLSTWLAMGFHGDMDYMAKHGIKRTRPNELVPGTSRVISVRMNYLPSDVKESWSVI
                     EDGHQAFISRYALGRDYHKVLRGRLQKLVEKMEVAIGKFNYRVFSDSAPVMEVEWASK
                     SGLGWRGKHTLLLSREAGSFFFLAEIYTDIPLPVDEPSKNYCGSCTRCLEVCPTNAIV
                     APYKLDARRCISYLTIELKGSIPETLRPLIGNRVYGCDDCQLICPWNKFAKPTQEPDF
                     AVRNGLDDSSLVALFAWTQDEFETKLAGSPIRRIGYQQWLRNLAVGLGNAPSSPSIIQ
                     ALKDRSNDPSTLVQEHVHWALKQHEAR"
     gene            complement(9597..9962)
                     /locus_tag="PK522_01430"
     CDS             complement(9597..9962)
                     /locus_tag="PK522_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HNC40426.1"
                     /translation="MKKIFASKLFLLLVIFFLLPTTSMAKDYPTKVGEKLGTGIANVV
                     TGVAEVPKTMMITGRKEGATYGMTAGLFIGMIHMAGRLFTGALDIATFPVPTTPIPNP
                     TFIWDDFDRETSYNAWRMR"
     gene            complement(10031..11716)
                     /locus_tag="PK522_01435"
     CDS             complement(10031..11716)
                     /locus_tag="PK522_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNC40427.1"
                     /translation="MTTSSTASNFIRNIIEDDNRSGKWNQRVETRFPPEPNGYLHIGH
                     AKSICLNFGLAKDYAGFCHLRFDDTNPEKEAQEYVDAICDSVKWLGFDWGKHIYYASD
                     YFEQLYAFAEFLIENGKAYVDSSSAEEIRQLRGTLNQPGINSPYRDRTIKENLDLFRR
                     MRAGEFPDGSHVLRAKIDMSSPNINLRDPVIYRIRHIHHQRTGDKWCIYPMYDFTHCI
                     SDALEKITHSLCTLEFEDHRPLYDWVLDQLQDKVPCHPQQIEFARLNLTYSVMSKRKL
                     IELVENKLVDGWDDPRMNTLAGIRRRGYTPEAIRLFTERIGISKADSWIDMTVLEDCL
                     REDLNERALRRIGVLNPLRLIIDNYPENQEEECYAPNHPQRPELGKRTLSFTKTLYID
                     QDDFMENPSKGYFRLSPGAEVRLRYAYIVKCTSIIKNDRGQIEAIHCTYDPDTKSGTP
                     GADARKVKGNIHWLSSKYARSAEIRLYDRLFTQPYPDAGEQDFKTYLNPDSKKIAMAY
                     LEPSLFEVQPEQIFQFERHGYFIADRVDTSPGKPVFNRAVTLRDTWGKIAGQG"
     gene            11897..13405
                     /gene="lysS"
                     /locus_tag="PK522_01440"
     CDS             11897..13405
                     /gene="lysS"
                     /locus_tag="PK522_01440"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNC40428.1"
                     /translation="MTEESELTPSQDENHIIAERRAKLAELRKRANPFPNHFRRDSFA
                     MSLQDTYCNQTKEDLEGQQISVKVAGRMMFKRLMGKASFATIQDMSGKIQLYVSNDHT
                     GEAAHEAFKHYDLGDILGAQGTLFRTKTGELSVRVTHLQLLTKSLRPLPEKFHGLTDQ
                     EQKYRQRYLDLITNENTRKVFIVRSKVIQAIREFLVARDYLEVETPMMHPIPGGATAR
                     PFSTHHNALDMPLYLRIAPELYLKRLVVGGMEKVFEINRNFRNEGISTRHNPEFTMLE
                     FYEAYQDYTYLMDLTESMLREVARAVMGTTHITYQEQEMDLAKPFAKLTITQAIQKYF
                     PEYSTAQLNDREFLISRLQALNIAYKPEDGVGGLQLSLFDETIEHLLFEPTFIIDYPA
                     EVSPLARRNDANPAITDRFELYIAGREIANGFSELNDPEDQAARFLEQAKAKDAGDNE
                     AMHYDGDYIRALEYGLPPTAGEGIGIDRLIMLFTDSPSIRDVILFPQLRKED"
     gene            13482..14168
                     /locus_tag="PK522_01445"
     CDS             13482..14168
                     /locus_tag="PK522_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40429.1"
                     /translation="MLSVQTENKHLLIWVVTVLIFFMIKEVSAQNYPPPEIIQGSRHA
                     INEQTNPKTQLADNPATQVLPPQPKTRSAVTYSTRSVLQKDSTDTSAHNSVRIRSVEA
                     GRIVRPYGQPACVNCGVINYVYPSGHARSGGGYGNAMAGIPGGVVAIPLGEDGEGRFI
                     APIGSILGNTSPYPNQISQGQTGGSVMYYDIGVMLENGTQTVVRQETKPHFNIGERVK
                     LIDGVLLPSP"
     gene            complement(14172..15350)
                     /locus_tag="PK522_01450"
     CDS             complement(14172..15350)
                     /locus_tag="PK522_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HNC40430.1"
                     /translation="MKAHSFNTQRRQLLRLSGLVLGTLLTQSFWTSRNSWASSTPLSS
                     VGPLQLSDKNGVRLPKGFTSRIVAASGQNLFGYRWHSAPDGGATFTTEDGGWIYVSNS
                     EIDKKMGGTGALHFNSEGTVINAYSILKNTSRNCSGGTTPWQTWLSCEEIERGYVWEC
                     DPFGKNKAQVRPELGCFRHEAIAFDVNSNKLYLTEDQPDGCLYRFTPYEMMDSGHVSL
                     DEGFLEVAEVIVSGHAKIIRWHRLPDPSAAAIETRKQVKHCTQFNGGEGIWYYGGKVY
                     FTTKGDNRVWVYNTQNHSLHIIYDASHYTSPVLIGVDDITSNELGELLVAEDKGNMQI
                     VILSGSTIKPLLQLVGHDQSEITGLAFSPDGSRLYFSSQRGKTGRNENGVTFEISGFR
                     "
     gene            complement(15362..16039)
                     /gene="pgl"
                     /locus_tag="PK522_01455"
     CDS             complement(15362..16039)
                     /gene="pgl"
                     /locus_tag="PK522_01455"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNC40431.1"
                     /translation="MLERRHYENINTLSTAVADFAADRLHTALQQNDVASLVIPGGST
                     PKFYLPTLARLPINWQKVMITLSDERWVDTRSQDSNEFLIRNCFLNCMENPARFIGLK
                     TTHDHPSLALLTINDRLSQLPLPINLAILGLGEDGHIASIFPGHSINSASNSLCEVAV
                     PPIAPSIRISLAFSLLASSDHIIVVVTGQNKRKRLEQFIDSQDPQIPFIRLVQQTHSP
                     ITIFESN"
     gene            complement(16033..16677)
                     /locus_tag="PK522_01460"
     CDS             complement(16033..16677)
                     /locus_tag="PK522_01460"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNC40432.1"
                     /translation="MLFLNWQAIIFDFDGVIVESGDIKTQAFGELYRSYGPEVEAAVM
                     EYHKQNGGMSRYRKFHYFQEQLLHQPPLTPQEEQDLDQRFSELVIEAVINSESVPGAE
                     ALIHNAYKHIPLFIASGTPEKELQRIVEQRGLASYFIDVRGSPQTKQALIAEILGAHA
                     LTPEKVLMVGDAMADYLGAKANNTAFLGRVRPGDTNPFPADVKVVSDLHPLLTC"
     gene            complement(16681..17625)
                     /locus_tag="PK522_01465"
     CDS             complement(16681..17625)
                     /locus_tag="PK522_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC40433.1"
                     /translation="MNNASVSDKMVVVFGASGFLGSHVADALSGAGYQVRLFDHISSP
                     YQRIDQEMIVGDIMDIDQVIEATRGAHAVYNFAAIADIDEAHNKPVATATINVLGNMH
                     VLEAARIAGANRFVFASSVYVYSESGSFYRASKQAAERFTETYHDRYGLDYTILRYGS
                     LYGRRADLRNGIYRMLHEAIQKHSITYHGSGNAMREYIHVEDAARMSVQVLSPEFANR
                     HLILTGQERMRIRDVMTMISEIMPWNVELHFEEANAISHYEITPYAFQPRIGRKLVLN
                     EHVDIGQGILDCIREIHQKICHTEDEDNAFPSTSDNKA"
     gene            complement(17618..18553)
                     /locus_tag="PK522_01470"
     CDS             complement(17618..18553)
                     /locus_tag="PK522_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNC40434.1"
                     /translation="MSKIVISTSSFDIQNNPYIERLTKRGMQIVLNPYQRKLSEDEIL
                     TLLGNDTIALLAGIEPLTKKVLTAARGLKVIARCGTGLDSVDLEAAHQCNILVSNTPE
                     APAQAVSELTMGLILSVLRQICSTHRLIQDGQWPRTQGHLLAAQTVGIIGLGHIGRRV
                     ATLCLAFGARVIAHDPYLQQAHPDIPLVNFEQLIKEANIISLHIPYSQGTHHLIDADT
                     LSRMKPGTVLINAARGGLIDENALVEALTSGHIAGAALDVFEQEPYQGPLLNCSNVVM
                     TSHMGSLAKESRQRMEHEAAENLLNGLITAGLLNE"
     gene            complement(18550..19311)
                     /locus_tag="PK522_01475"
     CDS             complement(18550..19311)
                     /locus_tag="PK522_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNC40435.1"
                     /translation="MSLKERLARSELTIGSWITLGHPAIAEIMASAGFDWLVLDMEHS
                     VMELSEAQIIIQVLDKQQCPAIVRLTSNHPDQIKRVMDAGATGVMIPMIKSAEDAKAA
                     VSSVYYPPQGQRGVGLARAQGYGARFQEYRQWLIENAVIVVMIEHIKAVEQIDSILAV
                     PGVDAYIIGPYDLSGSMGMPGELDHPQVQDAIKHVLDAGKRLQKPGGIHVIEPDSAAL
                     QQRIRSGFSFLGYSLDIRILDTICRTHLQSIKKQL"
     gene            complement(19326..20075)
                     /locus_tag="PK522_01480"
     CDS             complement(19326..20075)
                     /locus_tag="PK522_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNC40436.1"
                     /translation="MKIIAIIPARMGSTRFPGKPLANLLGRTMIEHVYKRVALSKFIS
                     ATYIATCDEEIRQAATAFGAPVIMTADTHERASDRIAEAVANIKGDLIVMVQGDEPMT
                     HPEMIDTAVRPFENDSQLGCVNLVRRIEQEADYHDVNTIKVVMNQQGDALYMSRQPIP
                     TLSKAGFSATQAYKQVCIIPFRRDVLLRYTQLAPTPLEKLESIDMLRLLEHGYPVRMI
                     ETQYNSQAVDTEADLARVAQLMATDPLLSQY"
     gene            20325..21605
                     /locus_tag="PK522_01485"
     CDS             20325..21605
                     /locus_tag="PK522_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40437.1"
                     /translation="MKALFFLRHYNDIDHITPIIAKWVECGHHCDVVLIGASRFRKDF
                     RIAYLSKLPGTRVNHIREVFSAWLFLKWRLQMLLLTGNLRRLFIGPFVAWLAKRYDAD
                     RRLPLWQETTNNLLSRCFDQTNQGIVAFDWIERNSVIAVEWVETVIGMARARGLGTVS
                     LPHGDSPHANQLIRRGEWKVGPDPVFSTARIFDRLAVPNELCAKRFEPFLPKQAIAVL
                     GSPRYCEEWLDKLSQLMPPSSLEHAERKLKIVMFLRKANFTTFWEEVSEVVHLLATFP
                     NVELIIKPHTRSGWKQSLTRSKSIKQLANVKIADDQMHSIQLMNWADVCIDLATSVVF
                     EAVRLKKPVLAADYLHAGRSAIAVYMPETELRCRDDVYEKIQRFLTEGTEDFYIESHR
                     QHFLDQMLDVGGKDVLAHYIALLESTAKLNEKKQ"
     gene            21640..22407
                     /locus_tag="PK522_01490"
     CDS             21640..22407
                     /locus_tag="PK522_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNC40438.1"
                     /translation="MLDFLKKLVVMAYMLKGGQTNVTVRNQPLVISVDHWRVLGRAKN
                     YSIKEPDTLDWLDGFEPGTCYFDIGANIGQFSLYPAKKYGQKVEIYAFEPQSNNYYAL
                     NKNIYLNQLKDSITAYCVAISGESKFDKLYVPKFIPGGNRSQFGEESQAAMGVPTSHI
                     QGMFGITLDDLCEKWNFPYPHYMKIDVDGIEISILRSASKVLKHPNLKSVIVELGTPL
                     EQQEAIAIMKQAGLELKHQSTRNWGETCFIFERTSTA"
     gene            22440..23705
                     /locus_tag="PK522_01495"
     CDS             22440..23705
                     /locus_tag="PK522_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40439.1"
                     /translation="MKALFFLRHYNDIDHMTPVIAKWIETGHICDVVMISKKRFDGDY
                     RIEYLKHLQGVRLAHIRDLLPVFDFFMWRLQTLLLVRSSYRSFLKPLVMFLATIFRQE
                     KRKKVWEKVSRYLLKRSFNDLNNGVVAFDWIERNSETAVEWVDVFVNLARNQGLGVVS
                     LPHGDSPHASQLIRHGEWLLKPDVSFSAAGMFDRVVVPNALCAIRFRPFLDDRKLVVL
                     GSPRYCEEWLTKLKSLLPESPLQNSRSRLKIVLFLRKANFTTFWEELSEVVQMLSTFE
                     EVELAIKPHTRGGWKQSFTKSAAIQHLKNVKIIESDIHSIHLIHWADVVIDLATSIAY
                     EAIRAKKPLLAADYLHAGRSAAAQYMPETELRCRDDVYERIHHFLQNGCENYYIEGNR
                     QKFITEMLESPGTDVLPHYVGLLEALASK"
     gene            23767..25005
                     /locus_tag="PK522_01500"
     CDS             23767..25005
                     /locus_tag="PK522_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNC40440.1"
                     /translation="MSEKQISMTYLLSERLISVEWRERFIRFSLIFFCFALWQKWSFF
                     YAFFLLSGAWILDGGLRNFKQLIREPLVQGILVFCSVVAIGLLWGDYIETNQNKWKKY
                     FILLTFIPFFALLNKDSARFPWVSNTFLIVYGSVLLVGAYQHFVQGLIGVPALDISYL
                     SFSAILGVGVITTIFLAQVSQTILQNMIWWFISALMLLLQFNQGARTVLFATMVAAII
                     LIFFMGQKKRKMRLIGISSFIGLALLLMATNSVMQERLSLVSHDWAAIKLGNYETSLG
                     YRLAIWDIGLNGIQEQPWIGHGTGNPEVYFEKETQTYKDGIYRNLPAFQKTSHYHNDW
                     IEVGMHWGGLGIMALAFLIFSWLRIFKENQLANLGIAFVSYIVLAGMTDTFLLYNRVP
                     PILLVITALLVCWQRSKMKS"
     gene            25061..25741
                     /locus_tag="PK522_01505"
     CDS             25061..25741
                     /locus_tag="PK522_01505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HNC40441.1"
                     /translation="MKRFEPLQSEHLVDLIPVNPAIECYMQGLIERRTDSVLVEMEAL
                     AKQKNFPIVGRLVGTSLEVLAKMINAKRVFEFGSGYGYSAYWFARAVGLNGQVICTDN
                     KLANKEQAEIFLSSVDLWKRVTFYIGYAQAVFSQTEGFFDICYNDVDKSEYPSVWKMA
                     REKIKPGGLYIADNVLWHGKVIAENNTDEWTQAILEHNQLIFDDPEFDAFINPTRDGV
                     MVARRKMA"
     gene            25817..26074
                     /locus_tag="PK522_01510"
     CDS             25817..26074
                     /locus_tag="PK522_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HNC40442.1"
                     /translation="MQIHVYDTYVKAKDGHIMHFDVFTSVRDDQKAIGYAKQWLTSIG
                     EEDAIVSSKECRFCHSQGAPDDVIEAINKDGYFIYKMEGCR"
     gene            26118..26633
                     /locus_tag="PK522_01515"
     CDS             26118..26633
                     /locus_tag="PK522_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HNC40443.1"
                     /translation="MKQVPFKRSLFIALFLACGLSHAADPKMIGEFNDWTAYVYMEGK
                     NKVCYMVSKPKKEEGSYTKRGSVYALLTHRPAENSKNVFSFMAGYPFKKESEVTVSIG
                     NQNFKLFTDNETAWAQDSDTDNKIANAIRNGSALIIKGVSARDTATTDTFGLKGSSSA
                     YGAISKECGIK"
     gene            26681..27028
                     /locus_tag="PK522_01520"
     CDS             26681..27028
                     /locus_tag="PK522_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HNC40444.1"
                     /translation="MDEVITIYQKPTCSKCRATLELLTQSGEEFQSINYYEDPISEDL
                     LRELLQKLNMTAREIFRSDEPAARGLQSATDEELLKRMAANPDLIQRPIVVRGEKAIL
                     ARPPEQVLQLLKK"
     gene            27268..27702
                     /gene="rplM"
                     /locus_tag="PK522_01525"
     CDS             27268..27702
                     /gene="rplM"
                     /locus_tag="PK522_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNC40445.1"
                     /translation="MKTFSAKPLEVQHDWFLIDATDKVLGRLATAIAHRLRGKHKPIF
                     TPHVDTGDYIVVINVDKIKVTGNKAEDKKYYRHSGYPGGIYETTFKKMHARFPARPLE
                     KAVKGMLPKGPLGYAMIKKLKIYAGDTHPHSAQQPKNLEIKA"
     gene            27706..28098
                     /gene="rpsI"
                     /locus_tag="PK522_01530"
     CDS             27706..28098
                     /gene="rpsI"
                     /locus_tag="PK522_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNC40446.1"
                     /translation="MAINYNYGTGRRKSSVARVFIKPGKGAFVVNDKPLDEYFSRETG
                     RMVVRQPLELTGNTANFDIMVNIHGGGESGQAGAIRHGITRALIDYDETLKPVLSKAG
                     FVTRDAREVERKKVGLRKARRRKQFSKR"
     gene            28176..29213
                     /gene="argC"
                     /locus_tag="PK522_01535"
     CDS             28176..29213
                     /gene="argC"
                     /locus_tag="PK522_01535"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNC40447.1"
                     /translation="MIKVGIVGGTGYTGVELLRILAQHPEVSLKVITSRQEAGLAVDE
                     LFPSLRKYVSLKFVDPEKAKLDKCDIVFFATPNGVAMRQARELVDAGVKVIDLSADFR
                     IKDISEWEKWYGMSHACPELVSEAVYGLPEVNRKQIKKARIIANPGCYPTAVQLGFLP
                     LIEAKVIDTNSLIADTKSGVSGAGRKAEVHTLFPEAADNFKSYGVPGHRHLPEIRQGL
                     SNVAGKLVGLTFVPHLTPMVRGIHATLYAKLMSIKTKVDLQTLFEERYKNEHFVDVLP
                     AGSHPETRSVRGSNYCRIAVHRPQGGQTVVVLAVTDNLVKGAAGQAVQNMNLMFGFSE
                     TLGIEHVPILP"
     gene            29279..>29574
                     /gene="erpA"
                     /locus_tag="PK522_01540"
     CDS             29279..>29574
                     /gene="erpA"
                     /locus_tag="PK522_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNC40448.1"
                     /translation="MQLNSLEENQAIQGQLVFTDSAASKVKQLIEEEGNPNLKLRVFV
                     SGGGCSGFQYGFTFDELENEDDLVIEKEKVKLLIDSMSYQYLIGAEIDYVENSQG"
BASE COUNT         8355 a         5753 c         6766 g         8700 t
ORIGIN      
        1 caccattgtt cgtgatttag agggtaatcc attgccagaa tcctgtggtg aagggattga
       61 agaagttctc gtcaaaccat cggcggctca aattaatcgt gacatgccca ttcgctttaa
      121 tgggcaacaa gtctggccac aaaattaaaa aagcttaggt ttgttttttt gtaatccgaa
      181 ttcctgaaga taaattccaa atcaatagag tcattgacaa gcgtaatggg cttgagtaca
      241 ttgatttctg ttctatcgaa aaataaattt ttcctagatc agtttgcgag acgttttgtt
      301 actaaaatta tggagaataa attatggtaa gacggacact cactgcattg gcagcctcat
      361 tattgcttct tcccgtttcg gttctggctg caggatatgg cccggcaggt tgtggtctag
      421 gttcgatggt cattggctct aaaccaggga tggaacaagt gtttgcggca acgacaaacg
      481 gaacatctgg ttctcaaacc tttggaatta cttcaggtac ctcgaactgt ggcgatcatg
      541 gccttgttaa tctgagcaag gaacgtgaag tgtttgctca ggaaaattat acgagcttag
      601 tcaaagaaat ggcgcaaggt aagggggaaa gcctctcaac cttagcgagc ttgtatcaat
      661 gccctgcaac gacacatggt gagtttggga cgctagttca ggaaaaattc gattatttag
      721 ttgcgaatga ccaaactact tcagccgaat tattgagcaa gctcgatggt cagttgatgc
      781 agcatcccaa tttagcgaat tcttgcaata tcaaattatc cgcacttaat taatcatgtt
      841 acggtagata tgaatttgtt tcatatctac cgattcagtg ggtgttgagc attactttgt
      901 gtcgtatcgt attctcagtt ttggtttagt tttctgtttt tttttatttt ctcatcttgc
      961 actagcgaat aatgaagaag ctctccgtta tttgatagag cttcaacaac aagcacagca
     1021 gaaaaagcta gcacaagccc gtgtttggca tcgattactc aaatacaatc cgcaactctt
     1081 acgtggtgtt atgagtgagg cggatgggct ggattttttt aatgcgcccc aaggaaaaat
     1141 agattctgac gctgaattag cagcaaccct cgcaagtttt tttttaccgc tttctgacgt
     1201 tcctgaaggt aaggagcatc ctcaatgtaa cttccccgcc cgttttaaat ggctcaatca
     1261 gcaactttca tttgatccac aacgactcga catacaaagc tgcgaccgtc tcgatcgttg
     1321 gttgggtgta ttagatcccg tgggggttac tttagttttt gcttcctatt ttatgaataa
     1381 cccggcctcg atgtttggcc atacactgat ccgcattgat agccggagaa caggccccga
     1441 taatcacttg atcaattatg gcgcaaacta tgcagcgata ccggatacgg ataatgcttt
     1501 tttatatgca ttaaaaggtc tgattggctc attccaaggg cgttttgcga tttttcctta
     1561 ttacaccaaa gtgcaagagt acaacaattg ggaaagtcgt gatctttggg aatatgaact
     1621 tcagtttaat gaagatcaac tacacatgct gttgttacac ttgtgggaac tggggggcac
     1681 ttattttgat tattattatt ttcaggagaa ctgttcttat catgtgctct cattgcttga
     1741 aattgccaat ccggatttat ttctgaaaga ttcgttcttt tttagtgtta ttccgacaga
     1801 tacggttaaa gtggttttgg cccaggaagg tctagtcaaa caagtcgttt atcgaccggc
     1861 tgtattgaat ttgatgaacg ataagcgcct aagaatgacg tctgatgagc gaaagctttt
     1921 cgaagcgttg atcaaaggta acataactat cgagaacccc tcgtttacag agcttcccgt
     1981 tcggtcacaa gcgaatctgc tcaacgccta catggattat cgccagtact acagtatgca
     2041 agaagatagc aaccatgaaa tcaagattcc acgcgcagtt ctattagcta ggagtcgatt
     2101 gccaccgact gaagacaatg atcaaaccat tcgtttttcg agtccacccc atcaagggca
     2161 tggcacagat cgctttcgtt taggctgggg acacaatgat cgtgagccat tcgtagagtt
     2221 tgcctatcgg cccgcatacc atgatttcat ggcaaaaaat gtgggttatg ataaaaactc
     2281 tgaaatcatt tttatggact tcaagcttcg gtactacctt gagtcagaac gcgtcagatt
     2341 ggatcaggct aaactcttgt cgattacttc gtttaatccc tatgacccgc tatttgcacg
     2401 tacatcctgg cgtggggaaa ttgagattga cacgctgaga gatcgggatt gccattattg
     2461 caattctttc aaagggagtt atggaagagg catggcttat cgcccccagt ttttctcccc
     2521 actattatta ttctctttta ttgatgttaa aactgaattt tcaccgcatc taaatcaaaa
     2581 ttatcgcttg ggtggcgatg ttgaggtggg cgcatactat gatattaccg ataatttacg
     2641 cgttaaactt gcagggagtt atcaggtttt tttcttagga gaaagcaaac gtttctttac
     2701 agcgcagttt ctaactcgat atgcgattgc gcaagatctc gatatcagaa tggaattgaa
     2761 ccgttacgac cataaccacg aaggaatttt cgctatcaat tattttttct gactgattga
     2821 tttaaatcga gctgggttag gaaatttaga aacactgtac gatatttctt ctcatgctgc
     2881 cagaaaatat cagtatggtt tccattggga atacgccata tcgatttggg ttcagagagc
     2941 ttttcataaa tggcttcacc gaattggggt tcgacaacgt tgtcttgttg tccatggatg
     3001 acgagcacgg ggactggcgc aattgtttcc aaatgagtgg gtgcccagcg atcgctgagc
     3061 aataaataac tcaacggttg caatggccaa gtaaaccagt gttgcgacag tttatgacgc
     3121 gcaatctgtt ggaatgaatc aaaagtactc tctgcgacca ctagctttaa gtttatatct
     3181 tcccgcttat cgatgactgt tcgtaacgcg atgatgccgc ccatgctttg tccatagaca
     3241 atcatgggaa gattttcagg atcatggcta agtacccaat cgagtgccgc atgacccgct
     3301 tctacacagc tttgcggtgt cggttttcct tcggacaatc cataaccggg gtaatcaaaa
     3361 ataaataagt tatagccttc agccgtgatc caggcgagat gcatgaaatg cgaggtcaaa
     3421 ttttctgcat tgccatgaaa aaaaataacg gttgctttcg caggcgtgat ggtcgagggg
     3481 aaccaccagc catgaagttt tcgttgagcg gcatcggtaa aatagatgtc agtaggggaa
     3541 aatccaactg acttagggtc atagtaaagt cgattctgac taggataata gagaacactg
     3601 gagcaactgg tcagtaacca tgtactcagt aacatgagta aacgaaatgc tttcaattta
     3661 agtatcagcg tggccctttg ctctaatacc gggttgtcaa aataagaatc gcgagttcag
     3721 aacataacac aactacgcag tttcttgagg cattttgtta atggcattac cttccagatt
     3781 aatatgttct atggagaatt atatgtagag cgttcggtgg cgttgtatct attcgcgatt
     3841 gcttgcaggt ggccagatcg gtaccgtaga tgcgcgacat ctggattgaa ttttaaccgc
     3901 caactggagc gattgaagtt agtctgatga tatttgtgct actggataga gttgtgacgg
     3961 agaacggcaa gcagggctaa taccaagctt gccgtttgtt catgaatcaa ttaactgatt
     4021 ttcttgatgc tgcgacgagt gaaagccaaa ctaacaagac caagaccaag aagtgcaacc
     4081 gaagtgggtt caggaagttc tgaaatcaca atatcgaatg cccaagcact tgttcgtgta
     4141 tttccatcgg catcgataaa cccggttact cccgacccgg taaatccatc tgccagattc
     4201 gggccaattg caaaattggc atactgtgtc aaagatgctg tgtagtttcc tatatcgaga
     4261 aaaatgctaa gcacggcatc aaattcagcg ccagtaacag gatcggctgc tactcctgcg
     4321 ccatcatcat tatcatcgat caaattacct gtgccattaa aaagcgtaat aataggatcg
     4381 aagccacccg cagatatagt atttccctca gcatttgttc cacctgcgta agaccatgtg
     4441 cgaagtgtaa caagtgatgg ttcagtcaca gcaaaactga agaactctac gtcatcatca
     4501 gtgttaaagc taccagtaaa cgaagaactg ataggagcag cgccaacttg agcaactgat
     4561 aaagcgaata ctatacatac agcaagaaac atttttttca tttaaatctc caaatatggt
     4621 taaaaagctg cagatttaac cataacgcag ttatttctaa agcaaatttt atgccattct
     4681 aattacttgt tgattaacaa atgcttaagc ttattttcga tagatgaatc actagcagtg
     4741 taaattaatc cgacagtaac taacgattag gcatcaatta tttggaaaat gaattatgtt
     4801 tttattagaa gaagcatgtt atagaggtat aaatgatctc acggcccaaa agtaaaatgt
     4861 tgcatatgag agattcaggc aatgagcata gcaagtgtaa aaaatattga catcttggta
     4921 taaatgcgca gaaaaaggcc tacacttcca tgatttcaaa tgcttactga ttgtatgact
     4981 gtacgcgttg gtagtggcta gctagaatca tttaagaata tcccatttcc tcttatcatg
     5041 gcggtgatcc tgtaagttta agcttagcca tattcataaa ctgagcatga tatcgtgatg
     5101 gtcttaacat tgaaagcgtt agcaacctgc actatgattc ggtgttattt aaggatatgg
     5161 aaataaaatg atcaattggt cacaaattct gatgttggca aaattgggta atccccctcc
     5221 atctcagcgc gtagaaaaga cagaagctga atggaagtcg ctgctcaccg atgagcaata
     5281 tttcgtttta cgccagaaag gaaccgagcc ccctcacagt tcaaaattat gtacgcaatt
     5341 cgagcctggt ttatatgcct gtgcaggttg cggcacggaa ttattcgatg catcatccaa
     5401 gtttaacagt ggaacaggat ggcctagttt tacgcaaccg gttgcagaga atgtagtggc
     5461 ttattatctt gataccagtt atggcatgca gcgtatagaa gtgaattgta atatttgcgg
     5521 tgggcatttg ggccatgctt ttcctgatgg tccagaaccg agtggactaa ggttttgtat
     5581 taacgcgctt tcgctcaaga aagtataact ggctttaagg aacatcaatt tctcatgacg
     5641 ctgcagcata atcccttgat agcatctcaa ttaccgattc gactcgttat tcatggcggt
     5701 gctggtgcta tttcgcgtgc tcgctttaca aaagagcgtg agcaaactta tcagcagaca
     5761 cttcgagagg cattactcgc aggctatgct gtgttgaatg ccggtggaag tagtattgaa
     5821 gcagtgaccg aagcaattgt cgtcatggaa gattctccac tctttaatgc agggaaaggg
     5881 tcggttttta cccatgaagg ccataatgaa ctagatgctg cgattatgga tggtgctaac
     5941 cgtatggctg gtgcggtagc aggggttaaa acgattcgta atcccattcg tgctgcacga
     6001 gcggttatga ctaaaagcaa ccatgtcatg ctgattgctg aaggggcgga agcttttgct
     6061 cgcgatcaaa ggctagagat cgttgacgcg acttatttct atactcagca acgctgggag
     6121 caattgcaaa gaacgattga gagagagcgt gtgcagcacc aacaggcaat agaagctgat
     6181 tcgttgttgg ttgagaaaga tgataaatat ggaacggtcg gtgcagttgc gcttgatcgc
     6241 agtggaaatt tagcggcagg gacttcgact ggaggacttg tcaataagcg ttatggacgt
     6301 gttggcgatt caccgattat tggcgctggt acttatgcgg ataatcgatc agtggctgtt
     6361 tcgggcacgg gtactgggga gatgttcata cgaaccgctg cagcacatag cattgcaaca
     6421 cgagtgcagc taaagcaact atcaattacc gaagccgcgg atcaaatact agccgaaatt
     6481 gctgcaatcg gtggtgatgg tggggtcatc gtgctcgata agagtggtca ttacaccatg
     6541 cgttttataa cttcaggtat gtaccggggg acaatcggcg gggacggagt gccttgggtg
     6601 ggaatttttc cagaagcgga gattgctttg aatgctaaaa aatagtcgtg gatcgatttg
     6661 gagaatcaat ccttcgaagc gtgatgcttc ctaatttacc tttgctaatt cagtcctggt
     6721 tggaattgcg cggctattac taaaatagcg tttaattccg gaaacaatcg cgctagccat
     6781 tctatcttga tagttttcgc tttttaattt ctcttcttca gttggattac tgataaatgc
     6841 agtttcaact agaatggatg gaatatccgg agatttaagc actgcaaacc ctgcttgctc
     6901 tacttggcgt ttatggagat gattaacgtt gcctatcgct gaaagcacgg taccagcaag
     6961 tttaatactg tcattaatgg tggcattgat cgataaatca atgagtgttt ttctaaggta
     7021 atggtcttta gtatttaact taattccacc gatccggtca gattcatttt ctttttgcgc
     7081 tagccagctt gcggtagttg aagtggcacc attttcagat aatgtataaa cggaagatcc
     7141 acgcgcatca ttcctaggaa ctgcgtcggc atggacagaa atgaacaagt ctgcatttgc
     7201 actgcgggcc tttgtgcgac gaactgcaag cggtaagaaa taatccccgg tacgcgtgag
     7261 taccgcgtgc atacccggtt ctttatcaat tttagctttt agcttcttgg caattgaaag
     7321 cgtaatatct ttttcttgtg aacctctggg cccaattgcc cctggatctt ttccaccatg
     7381 acctgcatca atggcaacaa tgattgtttt ttgtttttcc ggtctaggat tgaagctggc
     7441 taaggttatt tctggtttat tagcgttttt agctttagtc gctatttttt cctctgatgc
     7501 tttaacaagt gccattaacg gatcatcgtg gtgcccattt ggttttgtcg cagcgcgatc
     7561 cggtggaaat atatcgagaa ccagacgata gccgaatttg tccatcgggt ccaaggtgaa
     7621 ggttctaggc acagccttcg ttttaagtgc taaggttaac cgaaccccat ttgaagataa
     7681 atattcaatg tgagccttct ttattaaagg atctttggca tcaatttttg tagggagact
     7741 tcctagtggt ggatttaggc cggtattctc aagatcgagt acgatttgat cagggctgct
     7801 ttgtaagcta agcgtatact taatcggtag attggattca agggttaccc gggtatattc
     7861 aggcccggcc caaactcgcg tggaagtaac ttgcgcactc gaatttgcat gcactgtaaa
     7921 tgtcaacacg cttaaacaca atagaacaag ccctaccagt ataaactgat agtaatggct
     7981 aatgcgcggt ttttttatca tgctagcaat gctaggcatt ttttaccctg agtggtattc
     8041 gcgcaaattc cagcgaatcg acccctgttg gcaaattgaa gttttatcgt taaatctgct
     8101 gttggcaata aatctccccc tttttctggc cattcgacta aacagatcgt ttcagaatta
     8161 aagtaatcac ggaaaccagc tgcttcccat tccgaataat cattgaatct ataaaaatca
     8221 aagtgataca agtataacct agaaaaatta tagatttcaa ccaaattata tgtcgggctt
     8281 ttgacttttc cttgataacc catgccacgc agtattcccc gtgttagcgc tgtttttcca
     8341 gcaccgaggt caccctttaa aaaaatgatt aagccggcat gaagcgtagt tgcgattttt
     8401 gcgccgaatg ctaaagttgc agcttcgtca attaaatgta agcaatattg ctgctcctca
     8461 tttggcacag cctctgaaca atcacgatta tgcaagaaga aatctcccag aatggaacag
     8521 ttgatccggt ttcactcaaa caatcgataa ccaattgggc ccatcaactg ggttttcaag
     8581 atatccggat cgccaatgct gaggcggata tgtcatcagt tgaaacaggg ctgtctacat
     8641 ggttagcgat gggtttccat ggtgatatgg attatatggc aaaacacggc attaagcgca
     8701 cacgaccgaa tgaactggtc cctggtacat cgcgggttat ttcggtacga atgaattatt
     8761 tgccgagtga cgtgaaagag agttggagtg tgatcgaaga tggccatcaa gcgtttattt
     8821 cccgttatgc tctcgggcgg gattaccata aagtgctgcg gggtcgtttg caaaaactcg
     8881 ttgaaaaaat ggaagtagcg atcggcaaat ttaattatcg cgtgttttcc gatagcgctc
     8941 cggtcatgga agtagaatgg gcatcaaaat cagggcttgg atggcgtggt aagcatacat
     9001 tgctcttgtc tcgcgaagcg gggtcttttt tttttctagc agaaatttat acggatatac
     9061 cgctccctgt tgatgagccg agtaagaatt attgcgggag ttgtacacgc tgcttggaag
     9121 tatgcccgac caatgcaatc gttgctcctt ataaattaga tgcacggcgt tgtatttctt
     9181 atctcacgat tgaactcaaa ggcagtatcc ccgaaacttt gcgtcctttg attggtaatc
     9241 gggtttatgg ctgtgatgac tgccaattga tttgtccctg gaataaattt gctaagccta
     9301 cccaagagcc tgattttgca gtccggaatg gcctagacga tagctcgctg gttgcattgt
     9361 ttgcgtggac tcaagatgag tttgaaacga agcttgctgg tagcccaatt cggcgcatcg
     9421 gttaccaaca gtggctcaga aacctggcag tagggcttgg aaacgcaccg agttcaccct
     9481 ccattattca agcgctaaag gatcgcagta acgatccctc aacgctagtt caagaacatg
     9541 ttcattgggc acttaagcaa catgaggcta ggtagcccat ttacaatatg caataactat
     9601 cgcatgcgcc atgcgttata tgaagtttcg cgatcaaaat catcccaaat aaaggtagga
     9661 ttgggaatgg gtgttgtagg aactgggaat gttgcgatgt caagtgcccc agtaaacagg
     9721 cggcccgcca tatggatcat accgatgaaa agtcctgcgg tcattccata ggttgctcct
     9781 tctttacgtc ctgtgatcat catcgtttta ggcacttccg ctacacccgt caccacattt
     9841 gcgattccgg tacctaattt ttcacctact ttggttgggt aatctttggc catactggtg
     9901 gtaggaagta gaaaaaagat cacgagtagc aagaagagtt ttgatgcaaa aatttttttc
     9961 atgttatttt tttcccaaaa aaacggaatc atacactatt gaattatcga ttggcttcga
    10021 tgattgaaga ttatccttga cctgcaattt ttccccaagt gtcgcgtaac gttacggctc
    10081 gattgaatac gggttttcca ggcgaagtat ctacgcggtc cgcaataaaa tagccgtgtc
    10141 gctcaaattg gaaaatttgc tcaggctgta cttcaaacaa tgacggttca agataagcca
    10201 tggcaatttt tttggaatca gggttgaggt aagttttaaa atcctgttca cctgcatcag
    10261 gatagggttg ggtaaataac cgatcatata gccttatttc tgcagaacga gcgtatttac
    10321 tggaaagcca atggatattt cctttaactt tacgcgcatc agcgccgggt gttccgcttt
    10381 tggtatcggg atcataagtg caatggatcg cttcgatttg tccgcggtcg ttcttgataa
    10441 tagaagtgca ttttacgata taggcgtagc gtaggcgtac ctcggccccg ggagataagc
    10501 ggaaatagcc tttgcttgga ttctccatga aatcatcttg atcaatgtaa agcgttttgg
    10561 tgaaagaaag cgtacgttta cctaattcag gccgttgtgg gtgattaggc gcatagcatt
    10621 cttcttcttg gttttctggg tagttgtcaa taatcaaacg aagtggattc agtaccccta
    10681 ttctccgtaa agcgcgctcg ttcaaatctt ctcgtaaaca atcctcgagc acggtcatat
    10741 cgatccaaga atctgctttg ctgatcccga tccgctcggt aaagagtcgt attgcttccg
    10801 gcgtatagcc acgccgacga atgcctgcta gtgtattcat tcttgggtca tcccaaccat
    10861 cgactagctt attctcaaca agctcgatca gcttacgttt gctcatcaca ctgtaggtta
    10921 gatttaatcg agcaaattcg atttgttgtg ggtgacaagg aaccttgtct tgcaactgat
    10981 cgaggaccca atcatacaat ggacgatggt cttcaaattc cagtgtgcaa agtgaatgcg
    11041 tgatcttttc taaggcgtcc gaaatacaat gggtaaaatc atacatgggg taaatacacc
    11101 atttatcgcc ggtacgttga tgatgaatat ggcgaatacg gtaaataact ggatcccgca
    11161 aattgatatt gggagaactc atatcaattt ttgctcgcaa tacatgactg ccatctggaa
    11221 attcacccgc acgcatacgc cggaataagt ctagattttc tttgattgtg cgatcacggt
    11281 acgggctatt gatccctggt tggtttaatg tgccccttaa ttgccgaatt tcctcggcag
    11341 atgagctatc aacataggct ttgccgtttt cgataagaaa ttcagcgaat gcataaagct
    11401 gctcaaaata atctgaggca tagtaaatat gttttcccca gtcaaaacca agccatttga
    11461 cagaatcaca gattgcgtca acatattcct gcgcttcttt ttctggattg gtgtcgtcaa
    11521 accggagatg gcaaaatccc gcatagtcct tagccaatcc aaaatttagg caaatacttt
    11581 ttgcatggcc aatatgcaag tagccatttg gctctggggg gaagcgagtt tcaacccgct
    11641 gattccattt gcctgaacgg ttatcgtctt cgatgatgtt acggataaaa tttgaagctg
    11701 tagaagatgt cgtcacaaaa attctttcga ttgattagat tgagaatgta gttatcagcg
    11761 aactatatat aatatggtat taatcgcttt atattatagt gcgtgattcg tttgataaaa
    11821 cgagaataag caaatagttg ggcatttgtt ttctgtacct agctaattat ccgtaattcc
    11881 tacctaatct aatcatatga ccgaagaatc cgagttaact ccctcccaag atgaaaacca
    11941 tattatcgct gagcgacgcg ccaaacttgc tgagctaagg aagcgagcca atccatttcc
    12001 aaaccatttt aggcgtgata gctttgctat gtcgcttcag gatacttact gtaatcagac
    12061 gaaagaagat ttggaaggac agcaaatttc cgtgaaagtt gctggacgaa tgatgtttaa
    12121 gcgcctcatg ggtaaagcca gttttgcaac gattcaggat atgagtggca aaattcagct
    12181 ctatgtgtcc aatgatcata ctggggaggc tgcgcatgaa gcatttaagc actatgatct
    12241 tggagacatt ctcggtgcgc aaggcacttt attcagaacc aagacaggcg aactttcagt
    12301 gcgtgtcacg catttacagt tattaacgaa gtcattgcga cccttacctg aaaaatttca
    12361 tggacttacg gaccaggaac aaaaatatcg tcaacgctac ctggatttaa ttaccaatga
    12421 gaatacacgc aaggtattta tcgttcgttc gaaagtgatc caagcaattc gggaattttt
    12481 ggttgctaga gattatcttg aagtggaaac accaatgatg catccaattc caggaggagc
    12541 aactgcgcga ccgttctcga ctcatcataa tgcactcgat atgcccctgt atctgcggat
    12601 tgctcctgaa ttatatttga aacgattagt cgtcggtgga atggagaaag tgtttgaaat
    12661 taatagaaac ttcaggaatg aagggatatc tactcgacac aatccagagt ttacaatgct
    12721 ggaattttac gaagcatacc aagactatac ctatctaatg gacttgacag aatccatgct
    12781 gcgtgaagtg gcgcgggctg taatgggaac gacacatatt acctatcaag aacaagaaat
    12841 ggaccttgca aaaccttttg caaaactcac cattacccaa gcgattcaaa agtattttcc
    12901 cgagtattcg acggctcaac ttaatgatcg agagttcttg attagtcggt tacaagcact
    12961 caatatagcg tacaaacctg aagatggggt gggtggcctc caactttcat tgtttgatga
    13021 aacgattgaa catttgttat ttgaaccgac ttttattatt gattatcctg cagaagtatc
    13081 accccttgct cgtcggaacg acgcaaatcc agcaattact gatcgttttg aattgtatat
    13141 tgcgggacga gagatcgcga atggattctc agaattgaac gatccagagg atcaggctgc
    13201 acggttcctt gaacaagcta aagccaaaga tgccggtgat aatgaagcca tgcactatga
    13261 tggagattat attcgggcgc ttgaatatgg tttaccacca acagcaggcg agggaatcgg
    13321 aatcgatcga ttaatcatgc ttttcacgga tagtccgagc atacgtgacg tcattttatt
    13381 tccgcaactc cgtaaggagg attgaactta ttcccattta acaaggcctt atttcagtcg
    13441 atgatatagt aacaattgat actagttcgg agaaactgat aatgttatct gttcaaactg
    13501 aaaacaaaca tttattgata tgggtagtca ctgttcttat ttttttcatg ataaaagaag
    13561 tgagcgcaca gaattatcca cctcctgaaa taatacaagg atcacggcat gcaataaacg
    13621 agcagactaa ccccaagacc cagctcgctg ataatcctgc aacgcaagta ttaccacctc
    13681 agcctaaaac gagatccgca gttacttatt ctactcgaag tgtgctccag aaagactcaa
    13741 ccgatacctc ggcacacaat agtgtacgta ttcgaagtgt cgaagcaggg agaattgtaa
    13801 ggccttatgg tcaacctgct tgtgtgaatt gtggtgtgat taattatgtt tatccttctg
    13861 gccacgcaag gagtgggggt ggatatggga atgcaatggc aggaattcct ggcggtgttg
    13921 ttgccattcc tttgggtgaa gatggggaag gtcggtttat cgcaccgatt ggtagtatat
    13981 taggaaatac ttcgccgtat cctaatcaaa ttagccaggg tcagactggt ggtagcgtta
    14041 tgtactacga tatcggggtg atgttggaaa atggaacgca aaccgtagtt aggcaagaga
    14101 ctaagccgca ctttaatata ggtgagcgag tcaaactgat cgatggcgtt ctattgccaa
    14161 gcccttaatt gttatcggaa tccgctgatc tcaaacgtaa caccattttc attccgcccc
    14221 gtttttcctc gctgtgaact aaaatagagt cgagagccgt cagggctgaa tgcaagtcct
    14281 gtaatttcag actgatcatg accaacgagc tgtaacaatg gcttaatcgt gcttcctgac
    14341 aaaatgacaa tttgcatatt gcctttgtct tcggccacga ggagttctcc tagttcattg
    14401 gaagtaatat catcaacacc tatcaataca ggggaagtat agtgtgatgc atcataaatg
    14461 atatggagtg aatggttttg cgtgttatag acccaaactc gattatctcc tttagtagtg
    14521 aagtatactt ttcctccata gtaccaaata ccttctccac cgttgaactg cgtacagtgt
    14581 ttgacttgtt tgcgtgtttc aattgcagct gcagatggat cagggagtcg atgccaacga
    14641 attatttttg catgacccga tacgataact tctgctactt ccagaaatcc ttcatctaag
    14701 gatacgtgac ctgaatccat catttcatag ggggtgaaac gatataggca gccatcgggc
    14761 tgatcttcgg ttagatagag tttattactg ttgacatcga aagcgatggc ttcgtggcgg
    14821 aaacaaccga gttcgggtct cacctgggct ttgttttttc caaatggatc acattcccat
    14881 acatagccac gttcaatttc ttcacacgat aaccaagttt gccatggcgt ggtgccgcct
    14941 gagcaatttc gtgaggtgtt ttttaaaata gaataggcat tgatcaccgt tccttcagaa
    15001 ttaaagtgaa gtgcaccagt accccccatc tttttatcga tttcactatt tgaaacataa
    15061 atccagcccc catcctcagt cgtaaacgtt gctccaccgt caggtgctga atgccatcga
    15121 tagccaaaaa gattttgccc tgaagcagca acgattcttg aggtaaatcc cttaggtaag
    15181 cgaactccat ttttatcgga aagttggagg gggcctactg atgaaagtgg tgtggaactt
    15241 gcccagctat ttcgagatgt ccagaaggat tgggtaagca gtgtaccaag cactaaaccg
    15301 cttaacctta ataactgacg gcgctgggta ttgaacgaat gagctttcat aagttttcag
    15361 gctaattgga ctcaaaaatt gtaatagggg agtgagtctg ttgaacaagc ctgatgaaag
    15421 gtatctgggg atcctgacta tcgatgaatt gctcaagacg tttcctttta ttttgaccgg
    15481 taacgacaac gataatgtga tcactggaag cgagcagcga aaatgccaag ctgatgcgaa
    15541 ttgagggcgc tattggtgga actgctactt cgcataatga attactcgct gagtttatag
    15601 aatgaccggg aaaaatagaa gcgatgtgac catcttcccc taagcctaat atcgctaagt
    15661 taatcggtag aggcaattgt gaaagccgat cgttgattgt caataaagcc agagaaggat
    15721 gatcatgagt ggtttttaaa ccgatgaagc gtgctggatt ctccatgcaa tttaagaagc
    15781 aatttctgat cagaaactcg tttgaatctt gtgaacgggt atccacccag cgttcgtcac
    15841 tcagcgtgat catcaccttt tgccaattta tgggcaagcg tgctaatgtt ggcaagtaga
    15901 atttgggtgt gcttccaccg ggaatcacta aactcgcaac atcgttttgt tgcaaagctg
    15961 tatgtaaacg atctgctgca aaatcagcga cagcggttga aagtgtattt atattttcat
    16021 agtgccgacg ctctagcatg ttaataaggg gtgtagatca gatacaactt tgacatcggc
    16081 tgggaaagga ttggtatcgc caggccggac tcgacctaga aaagcggtat tgttggcttt
    16141 tgcaccaaga taatcggcca ttgcatcacc aaccatcagg actttctcag gagtcagcgc
    16201 atgtgcacct agaatctcag caatcagtgc ttgtttggtt tgtggtgagc cacgtacatc
    16261 gatgaaataa gatgccagtc cgcgttgttc gacaatgcgc tgtagttctt tctcaggtgt
    16321 gcctgaagca ataaacaggg gaatatgctt ataagcattg tgaatgagcg cttcagcacc
    16381 cggaaccgat tcgctgttaa tgactgcttc aatgaccaat tcagaaaatc gttgatccaa
    16441 atcttgctct tcttggggtg ttagcggtgg ttgatgtagg agctgttctt gaaaataatg
    16501 aaattttcga tagcgagaca ttccgccatt ttgtttatga tattccatca cggctgcttc
    16561 aacttccggt ccataggagc ggtatagctc accaaatgcc tgtgttttga tatcaccgga
    16621 ttcaacgatg acgccatcaa aatcaaagat gatcgcttgc caattcaaaa atagcatcta
    16681 ttaggcttta ttatctgaag tggaagggaa tgcgttatct tcatcttccg tatggcagat
    16741 tttttggtga atttccctga tacagtcaag tattccctgc ccaatgtcaa cgtgctcatt
    16801 taggactagt tttcttccga ttcgaggttg gaaagcatag ggtgtaattt cataatgcga
    16861 aatcgcattg gcttcttcaa agtgcagttc aacattccat ggcatgattt cagaaatcat
    16921 tgtcattaca tcgcgtatcc gcattctttc ttgtcccgtt aaaatcagat gtcggttagc
    16981 aaattcgggg gagagaacct gaacactcat ccgtgccgcg tcttcaacat gaatatactc
    17041 tcgcatggca tttccgctac catgataagt gatactatgt ttttgtatcg cttcgtgtaa
    17101 cattcggtaa attccattgc gcagatcggc ccgtcgacca tataaagagc cgtagcgtaa
    17161 tatcgtataa tctaggccat atcgatcgtg gtaagtttca gtaaaacgtt cagcagcttg
    17221 cttgctggct cgataaaacg accctgactc tgaatagaca taaacactac tagcgaaaac
    17281 aaagcgattt gctcccgcga ttctcgctgc ttctaggacg tgcatgttac ccaatacatt
    17341 gatggtagct gttgcaactg gtttattatg agcttcatca atgtccgcga ttgcagcaaa
    17401 attatagaca gcatgagcgc cacgcgttgc ttcaataacc tgatcgatat ccatgatatc
    17461 gcctacgatc atctcttgat cgatgcgctg gtagggagaa gaaatatgat cgaacaatcg
    17521 aacttgataa cccgctcctg acaatgcatc tgccacatga ctgcctagga agccgctagc
    17581 accgaatacc acgaccattt tgtctgatac agaagcgtta ttcattgagt agacctgctg
    17641 tgattaatcc attcaataaa ttctcagctg cctcatgttc cattcgctga cgagattctt
    17701 tagccagtga tcccatatga gaagtcatga caacattact gcagttaagc aacggtcctt
    17761 ggtaaggttc ttgctcaaat acatccaacg ctgctccagc aatatggcca gaagtaagtg
    17821 cttccacaag tgcattttcg tcaatcaagc ctccccgtgc agcgttgatt agaaccgtac
    17881 cgggcttcat acgacttagg gtatctgcat caatgagatg atgcgtacct tgagaataag
    17941 ggatatgaag tgaaatgata ttcgcttcct tgatgagttg ctcaaagttg actaatggaa
    18001 tatcaggatg tgcttgctgt aggtaaggat catgtgcgat gactctggcc ccaaaagcaa
    18061 ggcacaatgt tgcaacgcgt cgaccgatat ggcccagccc aataatccca accgtttgag
    18121 cggcaagaag atgtccctgc gttcttggcc attggccatc ctgaattaat cggtgagtgg
    18181 agcaaatttg tcgcaacaca gaaaggatta gtcccatcgt taattctgaa actgcctgag
    18241 caggggcttc aggtgtattg gaaactagga tgttacactg gtgagcagct tctaagtcga
    18301 cgctatctaa accagtacca caacgggcga taactttgag gcctcgagca gcagtcaaaa
    18361 ctttcttagt gagtggctca ataccagcca gaagggcgat tgtatcgttg ccaagtaaag
    18421 tgagaatttc gtcttcactg agcttgcgtt ggtagggatt gagtacaatc tgcattcccc
    18481 tcttggttaa tcgttcgatg taagggttat tctggatatc aaacgatgaa gtagatataa
    18541 cgattttact cataattgtt tttttatcga ttgtaggtgc gtgcgacaga tggtatctaa
    18601 aattcgtatg tctagactat agcccaagaa actaaagccc gaacgaatgc gctgctgtaa
    18661 agctgctgaa tccggttcga tgacatgaat accgccaggt ttttgtaatc gcttacccgc
    18721 atctaaaacg tgtttgatcg cgtcttgcac ttgagggtga tctagctccc caggcatgcc
    18781 cattgaaccg gaaagatcat aaggcccaat aatataggcg tctacaccag gaaccgctaa
    18841 aattgaatca atctgctcaa ccgctttgat atgttcaatc atcaccacga tgactgcatt
    18901 ttcgattagc cattgacggt actcctggaa gcgggcacca tagccctggg cgcgtgctaa
    18961 tccaacacca cgctgtcctt gcgggggata atagaccgag ctcactgcgg ccttggcgtc
    19021 ttcagcagac ttgatcatgg ggatcatgac tccagttgcg ccagcatcca tgacgcgttt
    19081 gatttgatcc gggtgattgg atgtcagtcg aacgattgca gggcactgct gtttgtctaa
    19141 tacttgaatg atgatttgag cttcgctgag ttccattaca ctatgttcca tgtccagaac
    19201 cagccaatca aatccagccg atgccatgat ttcggcaata gcaggatgcc ctaatgtgat
    19261 ccaggaacca atcgtcaatt cagagcgagc aagcctttct tttagcgaca tcgtgtagtc
    19321 ctatatcaat attgagacag taagggatca gttgccatca attgagcaac acgagccaaa
    19381 tcagcttcgg tatcaactgc ttgggagttg tattgggttt ctatcattct gactgggtag
    19441 ccatgttcga gtaatcgaag catatcgatg gattccagtt tttccagcgg tgtaggcgct
    19501 agctgcgtat accgtagcag tacatcgcga cgaaagggaa tgatacaaac ttgcttataa
    19561 gcctgggtcg cagagaatcc agctttagat aacgttggaa taggttggcg cgacatataa
    19621 agtgcatccc cttgctgatt catgacgact ttaatcgtat tgacatcatg gtaatcagct
    19681 tcttgttcga tacgacgcac caagttaaca cacccgagtt ggctatcgtt ttcaaaaggt
    19741 ctgaccgcag tatcgatcat ttctggatgt gtcatcggct catcgccttg gaccatcacg
    19801 attaaatccc ctttgatatt agcaacggct tctgcaatcc gatcactcgc tcgttcatgg
    19861 gtgtcggccg tcatgataac aggtgctcca aatgcggtag cagcttggcg aatttcttca
    19921 tcacaggttg caatataagt tgcagaaata aatttactta aggccactcg cttgtagaca
    19981 tgctctatca ttgttctacc gagtaaattg gccagtggtt taccgggaaa gcgtgtcgat
    20041 cccatgcgag cgggaataat tgcaattatt ttcattagtc gatataaatt tttagggtca
    20101 aattactaac aataacagga aaaagcgttt gtagtgatgg gttgtattct aaaaggaagt
    20161 ttaagtctgt atgttcctga agatgataat ccgcgaattg attatgtttt ttggtaaata
    20221 aaatcgcgca cgtgtaaaat tacaaattca ttatttcaat tatcacagca acacctaaac
    20281 ttttttccta cgctttacgc atccactaca atcataacat aggtatgaaa gcgctctttt
    20341 ttcttcgtca ttataatgat atcgatcaca ttacaccgat tatcgccaag tgggtcgaat
    20401 gtgggcatca ttgtgatgtg gtgctgattg gtgcgagtcg ttttcgaaaa gattttcgga
    20461 ttgcctatct aagtaagctg ccaggaacaa gggttaatca tattcgtgag gttttttcgg
    20521 cttggttatt cttgaagtgg cgtttgcaaa tgttgttgtt gaccggtaat ttacgtcgtt
    20581 tattcatagg tccttttgtt gcatggcttg caaagcgtta tgatgcagat cgaaggctgc
    20641 cattgtggca ggaaactaca aacaatttat tatcgcgatg ctttgatcaa acaaaccaag
    20701 gtatcgtcgc ttttgactgg atagaaagaa attctgtgat tgcagtcgaa tgggtcgaga
    20761 cagtgattgg catggcacgt gccagggggc tgggtactgt ttcattgccc catggcgata
    20821 gcccccatgc gaaccagctt atccgcagag gtgagtggaa agtgggtcct gaccccgtgt
    20881 tttccacggc cagaattttc gaccgactcg ctgtgcccaa tgagttatgc gcaaaacgat
    20941 ttgagccgtt tttgccgaaa caagcgattg ctgtcttggg ttctccgcgt tattgtgagg
    21001 aatggcttga taaattgagt caattgatgc ctccctcgtc gctggaacat gcggagcgta
    21061 aactcaagat tgtgatgttt ctgaggaaag ctaatttcac gacgttctgg gaagaagtaa
    21121 gtgaagtggt tcatttgcta gcaacatttc ctaacgtaga attaattatc aagccgcata
    21181 cccgcagcgg ctggaaacaa tcgctcaccc gaagtaagtc gataaaacag ttagccaatg
    21241 tcaaaatagc ggatgatcag atgcattcta tccaactgat gaattgggca gacgtttgta
    21301 tcgatttagc gacctccgtc gtattcgagg cagtacgact caaaaaacca gttctcgctg
    21361 ctgattatct gcatgcgggg cgttctgcta tcgcagttta tatgccagaa acggaattgc
    21421 gttgccgaga tgatgtttat gaaaaaattc agcgatttct taccgaagga actgaggatt
    21481 tttatatcga aagccatcgc caacattttt tagatcaaat gctagacgtt gggggaaagg
    21541 atgtattggc acactatatt gcgctactcg aatcgacagc aaaactgaac gagaaaaaac
    21601 aataatggca gttatacccc gatttcattc aggaatttta tgctggattt tttaaagaaa
    21661 ctcgttgtaa tggcttatat gctcaaggga gggcaaacga atgtcactgt gagaaatcag
    21721 ccgctcgtga tttctgtaga tcattggaga gtcttggggc gcgcaaaaaa ttattcgatc
    21781 aaagaacccg atacactaga ttggttggat ggttttgaac caggaacttg ttattttgat
    21841 atcggtgcaa atattgggca attctcgctt tatccagcta agaaatatgg gcaaaaagta
    21901 gaaatttatg cttttgagcc ccaatcaaat aattattacg cactgaataa gaatatttat
    21961 cttaatcagc ttaaagactc cataacggcc tactgtgttg ccatatcggg tgaaagtaaa
    22021 tttgataaat tgtatgtgcc aaagtttatt cctggcggta atcgttctca attcggtgaa
    22081 gaatcgcaag cagctatggg ggtgccaact tctcatattc aaggaatgtt tggaattacg
    22141 ttagatgact tatgcgagaa atggaatttt ccttatcctc actatatgaa aatcgatgta
    22201 gatggcattg aaatttctat tcttagatca gcttctaagg tacttaagca cccaaatctt
    22261 aaatctgtca ttgtcgagct gggtaccccg cttgagcaac aagaagcaat cgctatcatg
    22321 aagcaagctg ggcttgagtt gaaacatcaa tccactcgta attggggaga aacttgtttt
    22381 atttttgaga gaaccagcac ggcatgatat ttcgctttat ctgttattgc tgattgtttg
    22441 tgaaagcact cttttttctt aggcattaca acgatatcga tcacatgacg cctgtcatag
    22501 caaaatggat cgagacgggt catatttgtg atgttgtaat gattagtaag aagcgctttg
    22561 atggtgatta ccgaattgaa tatctaaaac atttgcaggg agttcgtcta gcacatattc
    22621 gagacttact gccagttttt gattttttca tgtggcgatt gcaaacacta cttcttgtca
    22681 gaagttcgta ccgctcattt cttaaacctt tagtaatgtt tctggctacg atttttcggc
    22741 aagaaaaacg aaagaaagtt tgggagaaag tatcacgcta tttattaaag cgtagtttca
    22801 atgatctaaa caatggggtt gttgcatttg attggatcga acgtaattca gaaacggctg
    22861 ttgaatgggt tgatgtgttt gttaatcttg cacgtaacca aggtttgggt gtggtttcgc
    22921 tgccccacgg agatagtcca cacgcaagtc agttgatccg tcatggggaa tggttgctta
    22981 agccggatgt atcgttttct gctgctggaa tgtttgatcg agtggtcgtg ccaaatgcgt
    23041 tatgtgcaat acgctttcgt ccgtttctag atgatcgaaa gctagtggtg ctagggtcac
    23101 ctcgctattg tgaagaatgg ctaacaaaac ttaagtcatt attaccagaa tctccattgc
    23161 aaaattccag aagtcggtta aagattgtgc tattcctcag aaaagctaat ttcacgacat
    23221 tttgggaaga attaagtgaa gttgttcaaa tgttgtcaac gtttgaggag gttgaattag
    23281 cgattaagcc tcatacgcgg ggaggatgga aacaatcttt cactaaaagt gcggcaatcc
    23341 agcatttaaa gaatgtcaaa attatcgaga gtgatatcca ctcaatccac ctgattcatt
    23401 gggcagatgt tgtgattgat ctagctactt cgatcgctta tgaagcgata agagcaaaaa
    23461 aaccgttgct tgctgcagat tatcttcatg ctggacgctc agcagctgct cagtatatgc
    23521 cggagactga gctgcgctgt cgtgatgatg tttacgaaag aatccatcat tttcttcaga
    23581 atggctgtga aaattattac atcgagggaa atcggcagaa attcattacg gagatgttgg
    23641 aatctcctgg aacagatgtt ttgcctcatt acgttggact gcttgaagcg ttagcttcaa
    23701 aataattctt tcctatctaa aaattttatc tgaattcaag ctatcgttaa acgatgggat
    23761 accttaatga gtgaaaaaca aatcagcatg acctatttat tgagtgaacg tttgattagc
    23821 gtcgaatggc gggagcgatt tattcgattt tccttgattt tcttttgctt tgcactctgg
    23881 cagaaatggt cattctttta tgcttttttc ttgttatcag gggcatggat actcgatggc
    23941 gggctaagaa acttcaagca actgataaga gaaccgctcg ttcaaggaat attggttttt
    24001 tgtagtgttg tggcgattgg gttgttgtgg ggtgattaca ttgagaccaa tcagaataag
    24061 tggaagaaat attttattct cttaacgttt attccttttt ttgcgctgct gaataaagat
    24121 agcgcgcgtt ttccatgggt ttctaatacg ttcttaatcg tctacggtag tgtgttgtta
    24181 gtcggcgcct atcagcattt tgtgcaaggg ttaatcggtg ttcccgcatt agatatttct
    24241 taccttagtt tttctgcgat attgggcgta ggtgtaatta ccacaatttt tttagctcaa
    24301 gtgagccaaa cgatattgca gaatatgatc tggtggttca tttctgcgct aatgctgctg
    24361 ctacagttca atcagggagc aagaactgtc ttgttcgcaa cgatggtagc tgcaattatt
    24421 ctgatctttt ttatgggcca gaagaaaaga aaaatgaggt taatcggaat ttcgtcattt
    24481 atcggtttag cgctgctgct tatggctacg aattcagtga tgcaagaacg attgagtctc
    24541 gtgagtcatg attgggctgc gattaaacta ggcaattatg aaactagtct gggttatcga
    24601 ttagcaatat gggatattgg tttgaatggt atacaagaac agccttggat aggtcatgga
    24661 accggtaatc cagaggttta ttttgaaaaa gagactcaaa cgtataaaga tggaatttat
    24721 cgtaacttgc ccgcatttca aaaaacttcg cattatcata atgattggat tgaagttgga
    24781 atgcattggg gtggattggg tattatggcg ttggcttttt tgatattttc ttggttaaga
    24841 atatttaaag aaaatcaatt agctaatcta ggaattgcgt ttgttagcta tatagtactg
    24901 gctgggatga cggatacttt tcttctctat aatagagtac cgccgatatt attggtgatt
    24961 acagcattgc tggtatgctg gcagcgctca aaaatgaaga gctgaataag aaatgacaat
    25021 ctttgttacg ctagtgtagg actacataag ggatcctatc atgaaaagat ttgaaccgtt
    25081 acagagcgag catctagttg acttgattcc ggttaatcca gcgatagaat gctacatgca
    25141 aggattaatt gagagaagga cagattctgt attagttgag atggaggcct tagcaaagca
    25201 aaaaaatttt cccattgtag gtcgcctagt tggcacgtca ttagaagttt tagctaagat
    25261 gattaacgca aaacgtgttt ttgagtttgg tagcggctat ggctattctg catattggtt
    25321 tgcacgtgct gttggattaa atggacaggt gatctgcacg gacaataagc ttgccaataa
    25381 agagcaagca gaaatctttt tatcatccgt cgatttatgg aaaagggtaa ctttctatat
    25441 cgggtatgct caagcggttt tttcccaaac ggaaggtttc tttgatatct gctataacga
    25501 tgtagataaa agtgaatatc cgtcagtatg gaaaatggct agagaaaaaa tcaaaccagg
    25561 tggtttatat attgcagaca atgtactctg gcatggaaaa gtaatcgcag aaaataatac
    25621 cgatgaatgg acgcaagcaa ttctggagca taatcaattg atttttgacg atcctgagtt
    25681 cgatgcgttt attaatccaa ctcgtgatgg ggttatggta gcaagaagaa aaatggcata
    25741 ataagcgcac tggattcagt cgattctgta attgaattga tttttattta cgcattaatt
    25801 ttttaaggat agatttatgc aaattcatgt ctatgatact tacgttaaag ctaaggatgg
    25861 tcacatcatg cattttgatg tatttacttc cgttagagac gatcagaaag cgattgggta
    25921 tgcaaaacag tggctaactt cgattggtga ggaagatgct attgtttcaa gtaaggaatg
    25981 taggttttgt catagccaag gagcgccaga tgatgtgatt gaagccatta ataaagatgg
    26041 ttatttcatt tacaaaatgg aaggatgcag ataacttctg ggcagttagt tattaaccca
    26101 attccaagga ttgatagatg aaacaagtac cttttaagcg gagtcttttt attgctttat
    26161 tcttagcatg tggattgagc catgctgctg accctaaaat gataggtgaa tttaatgatt
    26221 ggacagccta tgtttacatg gaaggtaaga ataaagtttg ttatatggtg agtaaaccta
    26281 aaaaggaaga agggagctac acgaaacgtg gtagcgtata tgcgctgttg acgcatcggc
    26341 ctgctgaaaa cagtaaaaat gtttttagct ttatggctgg gtaccctttt aagaaagaaa
    26401 gtgaagttac ggttagtatc ggtaaccaaa actttaagct ttttacagat aatgagactg
    26461 cttgggctca agatagtgat acggataaca aaatcgcgaa tgccattcgt aacggcagtg
    26521 cgttgatcat taaaggtgtg tctgctaggg atacagcaac gacggatacg tttggattga
    26581 agggttcgtc atcagcttat ggcgcaattt ctaaagagtg cggcattaag tagcagcaat
    26641 ttattaatat gcgtcttcgt gtttaggatt aatagtttaa atggatgagg tcataacgat
    26701 ttatcaaaaa ccaacttgca gtaagtgcag agctaccctt gaactgctta ctcaaagtgg
    26761 tgaggaattc caaagtatta attattatga agatcctatt tcagaggatt tattgcgcga
    26821 gcttcttcaa aaactcaata tgacagcacg cgaaattttt cgatctgatg agccagcggc
    26881 tagaggtttg cagtcagcga ccgatgagga gctattgaaa cgaatggctg caaatcctga
    26941 tttgattcag cgacctattg ttgtcagggg tgagaaagcg atcttggcta gaccacccga
    27001 acaagtttta cagttattaa aaaaatagga ttgcttcatt gcgctatgta tctggatttc
    27061 atcgatttaa actaatcagc aagcttatag gatcattttt gagtgattgt tttatcctag
    27121 ttgtaacgga tggctaatgt agggtaaaat cggcggtttg gatttcagat taaattagca
    27181 gcgattattc tatttcataa gatattttgg ttaaatagaa tttagaatgt taatttaaga
    27241 aattatcgta aagtgagaga tacttaagtg aagacctttt cagcaaaacc attagaagtt
    27301 caacatgatt ggtttctaat cgatgcaacc gataaagttt tagggcgact ggcaacagcg
    27361 atcgcacatc gtttacgtgg aaaacataaa cctattttta ctcctcatgt tgacactgga
    27421 gattacatcg ttgttatcaa tgttgataaa ataaaagtaa ctggaaataa agcagaggat
    27481 aaaaagtact atcgtcatag cggttatcca ggaggaattt atgaaacaac ctttaagaag
    27541 atgcatgctc gttttcctgc aagaccgctt gagaaagcag taaaaggtat gctaccaaaa
    27601 ggaccgctgg gatatgctat gattaagaag ctgaaaatat atgcaggtga tacgcatccc
    27661 catagcgcac aacaaccaaa aaatttagag ataaaggcgt aatttatggc gataaattat
    27721 aattatggaa cgggtcgaag aaaaagctca gttgctcgag tatttataaa gccaggtaaa
    27781 ggtgcttttg tcgttaatga taaaccactc gatgagtatt tttctcggga aacgggtcgc
    27841 atggttgttc gccaaccgct ggaattaacg ggaaatactg ctaattttga tataatggtt
    27901 aatattcatg gcggaggaga atctggtcag gcaggcgcaa ttcggcatgg tattacacgt
    27961 gcgctcattg attatgatga aacattaaaa ccggttctta gtaaggcagg gtttgtaaca
    28021 agagatgcgc gtgaagtgga acgcaaaaaa gttggtctgc gtaaagctag gcgacgtaag
    28081 cagttctcta aacgttaaac gtatctgttt ctcattaaag ccgcctttat caggcggctt
    28141 tttattttgt acattttatt tttcattgat tcgatatgat taaagtaggg atcgttggtg
    28201 gaactggtta tacaggagtc gagttgctgc gtatcttagc gcaacatcca gaagttagtc
    28261 tgaaagtgat tacttctcga caagaagcag gtttggctgt cgatgagtta tttccgagtt
    28321 taagaaaata tgtttcatta aaatttgttg atcccgaaaa agccaagcta gataaatgtg
    28381 atatcgtgtt ttttgccact cccaatggag tagctatgcg ccaagctcga gagttagtgg
    28441 atgcgggtgt taaggtgatt gatctatcag cagattttag gataaaagat atttctgagt
    28501 gggaaaagtg gtatggtatg tcgcatgcct gccctgaact tgtttctgaa gcggtatatg
    28561 gattaccaga agttaatcgc aaacagataa aaaaagcacg tatcatcgca aatcctggtt
    28621 gctatccaac agcggttcag ttagggttct taccattaat tgaagctaag gtaattgata
    28681 ccaattcatt aattgcagat acaaaatcag gtgtatcagg cgctggtcga aaagcagaag
    28741 tacatacact ttttccggaa gcggctgata attttaaatc ttatggcgta ccgggtcatc
    28801 gtcatcttcc ggaaattcgg cagggtttgt ccaatgtagc gggtaaactg gtgggcctaa
    28861 catttgttcc acacttgact ccgatggttc gtggaattca tgctacgtta tatgcgaaat
    28921 tgatgagtat taagactaaa gttgatcttc aaacattgtt tgaagaacgc tataaaaatg
    28981 agcattttgt tgatgttcta cctgcaggca gtcatcctga gactcgttca gtgcgaggct
    29041 ctaactattg tagaattgcg gttcatcgac cacagggcgg acaaacagtc gtggttcttg
    29101 cggttaccga taatttagtt aaaggagcag ccggtcaggc tgttcaaaat atgaatttaa
    29161 tgtttggttt ttctgaaaca ttagggattg aacatgttcc aatattaccg taaagcaaac
    29221 ttaattcgtt tttttgctta caaatatcaa tgaataactg cgaatgggag gagcatagat
    29281 gcaattaaat tctttagaag aaaatcaagc gatacaaggc caacttgttt ttactgatag
    29341 tgctgcgagt aaggttaagc agcttatcga agaagaggga aatcctaacc taaagctaag
    29401 ggtttttgtc agtggtggtg gatgctctgg ttttcagtac ggttttactt ttgatgaatt
    29461 agaaaatgaa gatgatttgg taatcgagaa agagaaagtc aagttgctga ttgattcaat
    29521 gagctaccag tatttgattg gtgctgaaat cgattatgta gaaaattctc aagg
//
LOCUS       DAOQLJ010000010        28540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_527_length_28540_cov_6.533860, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000010 DAOQLJ010000000
VERSION     DAOQLJ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 28540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28540
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_527_length_28540_cov_6.533860"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="PK522_01545"
     CDS             complement(<1..736)
                     /locus_tag="PK522_01545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480962.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNC40449.1"
                     /translation="MQLNPSEISELIKSKIEGLSASSEIRTQGTIVSLTDGIVRIHGL
                     SDVMQGEMLEFPGNTYGLALNLERDSVGAVILGSYEHLTEGDTVKCTGRILEVPVGEG
                     LLGRVVNALGQPIDGKGPISAQGKEPIEKIAPGVIWRKSVDQPVQTGLKAIDAMVPVG
                     RGQRELIIGDRQTGKTAVAIDAIINQKGENMICIYVAIGQKASSIANVVRKLEEHGAM
                     AYTIVVVASASESAAMQFIAPYSGCTM"
     gene            complement(748..1284)
                     /locus_tag="PK522_01550"
     CDS             complement(748..1284)
                     /locus_tag="PK522_01550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNC40450.1"
                     /translation="MAESITIARPYAEAVFKLAKEKNSLSHWSEMLSNAAQITEVAPV
                     QELIGNPNVSASKLKEIIFNIGGSKLDGEVKNLLSILIDNDRVTMIPRLVEMYEQLRA
                     NHEGVLEANIISAFSMSDRQLEKLVAALENKFNRKVKAKVSIDQELIGGVKIEIGDEV
                     IDSSISGKLEAMSIALKS"
     gene            complement(1284..1757)
                     /locus_tag="PK522_01555"
     CDS             complement(1284..1757)
                     /locus_tag="PK522_01555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNC40451.1"
                     /translation="MNINFTLISQAIAFSVFVWFTVKFVWPPLLRAIEERQKQIADGL
                     AAGERGRKELELADQRSSDVLKDAKQRAADIVTQAEKRAAEIIDEAKSVAKVEGDRII
                     AGAKAEIAHEIFSAKEELRQQVSGLVLTGATKILRREIDAKAHADLLTAIETELK"
     gene            complement(1793..2065)
                     /gene="atpE"
                     /locus_tag="PK522_01560"
     CDS             complement(1793..2065)
                     /gene="atpE"
                     /locus_tag="PK522_01560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HNC40452.1"
                     /translation="MENLQYLAMIQAYTGIGIGLMIGLGAAGACIGVGVMCSRFLEGA
                     ARQPEMIPTLQGKVFLLLGLTDASFIIAVGLAMLFAFGNPLLAVVQ"
     gene            complement(2121..2924)
                     /gene="atpB"
                     /locus_tag="PK522_01565"
     CDS             complement(2121..2924)
                     /gene="atpB"
                     /locus_tag="PK522_01565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNC40453.1"
                     /translation="MAAELTPTSYINHHLTNMTTTVGDGSFWTLHLDTLVMSVMLGIM
                     GMGFLWLIVRKATPGVPSKTQAFVELLVEFIDNQVKNTFHGNRHVFIAPAALTIFVWV
                     LLLNAMDLLPIDIMAWVYEHVFGLHNWRSVPTTDVNTTFALALSIWLLTIFFSIKVKG
                     LGGWMHELFCTPFGTNIILWPLNLLFNMIEYISKPLSHSLRLFGNIYAGEIIFLLLGM
                     WAATGVSGAFFSAILGAGWAIFHILIVSLQAFIFMMLTVVYLAMAHESH"
     gene            complement(2947..3324)
                     /locus_tag="PK522_01570"
     CDS             complement(2947..3324)
                     /locus_tag="PK522_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HNC40454.1"
                     /translation="MSWIRNEPLRRVVFWQTLATGLATLVCGWLMGMHGAISALLGGL
                     VSIAASLAYAVLIDRHGGYTAGDVVKTALKAEAIKISIMIVLLLGVFLLYKEVVPIVF
                     IGVFIVAAIIFSMALFVTTAKNK"
     gene            3505..3825
                     /locus_tag="PK522_01575"
     CDS             3505..3825
                     /locus_tag="PK522_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNC40455.1"
                     /translation="MLLTTTPMIEGKRITQYCGIVAGEAVLGANVLKDLFAGIRDFIG
                     GRSGTYEKELQRARDIALNELKDKAKELGANAVIGIDIDYEVLGKENGMLMVSASGTA
                     VIFE"
     gene            4131..4532
                     /locus_tag="PK522_01580"
     CDS             4131..4532
                     /locus_tag="PK522_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40456.1"
                     /translation="MLVKYNLNHQAVTYYWKPISRYQQFQLVGEFYYQSAFKKLVENH
                     GNRGVYFECLATLIPENTNAFDSQAIRIDIKGMTVGYFNRNDARKFRIRLNTNKLAGQ
                     ITSCNAVIEGGRLLNDGRRSNYGVSLAINQL"
     gene            complement(4555..5349)
                     /locus_tag="PK522_01585"
     CDS             complement(4555..5349)
                     /locus_tag="PK522_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNC40457.1"
                     /translation="MTYVKLTEVVECMLDAGTKKANLSIKDMLIRGALAGAFLGYATS
                     LAVLVIVQTGLGIWGAIVFPVGLVMIVLLGLELATGNFAVIPIAVKDGRTRIGRLFHN
                     WFWVLVGNLIGGVGYAFLFVLVITKLGHVDPINLDQAIKLVALAEQKTIAYAAIGFGG
                     MATAFISAMLCNWMVALGSVIAFTSTSTGGKIAAMWLPTMTFFALGYEHAIVNMFVIP
                     AGMMLGANITFLEWWTWNGIPVILGNILGGMVFTGLALYITTRGQG"
     gene            complement(5389..6600)
                     /gene="hemW"
                     /locus_tag="PK522_01590"
     CDS             complement(5389..6600)
                     /gene="hemW"
                     /locus_tag="PK522_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNC40458.1"
                     /translation="MVEPKTIAPVLNFQSPHLLELPPLSLYIHFPWCIKKCPYCDFNS
                     HEIGSGANDLPEKEYLSALLRDLEAALPQIWGRSISSIFIGGGTPSLLSASGLEQLLS
                     QVRMLLLLEPSVEITLEANPGTFESKKFEAFREAGVNRLSIGVQSFNTEQLLKLGRIH
                     NANEARHAIEISQHYFDNINLDLMYALPNQTIEEARVDIETACTTGVPHISAYHLTLE
                     PNTMFYQYPPQLPDDDLSADMQALIEQTLKSSGFAQYEISAFAHAGHRSHHNLNYWQF
                     GDYLGIGAGAHSKISFRDKIVRQMRYKHPKKYLEVLLSQSVTPIQMEQELEVADRIFE
                     FMMNSLRLIEGFRLEDFRSRTGLPIIAAQQQLKVAEQMGLIHRDCHRIVPTDIGRKFL
                     NDLLQIFLPNK"
     gene            complement(6606..7199)
                     /gene="rdgB"
                     /locus_tag="PK522_01595"
     CDS             complement(6606..7199)
                     /gene="rdgB"
                     /locus_tag="PK522_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNC40459.1"
                     /translation="MQQLIIASNNTGKLREIEQILVPLEIQILPQKDLGVTEIEEPYQ
                     TFVENALAKARHASEQTGLPALADDSGICVQSLGGAPGIFSARYAGEPKSDQRNNQKL
                     IETLKFNSDRAAYYYCIMVLIRSANDPQPIIADGYWFGEIILEPRGEGGFGYDPHFFV
                     PELGKTAAQLTSEQKNQYSHRSKALVKLTQMLVNCRF"
     gene            complement(7207..7929)
                     /gene="rph"
                     /locus_tag="PK522_01600"
     CDS             complement(7207..7929)
                     /gene="rph"
                     /locus_tag="PK522_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNC40460.1"
                     /translation="MQRKNNRATYQIRPISVTRRYTKYAQGSVLIECGDTKVICTASI
                     SDKVPPFLKGSGQGWLTAEYGMLPCSTHERIQREASKGKQSGRTMEIQRLIGRSLRAI
                     TNLALIGERTIQIDCDVIQADGGTRTASITGAYVALHDAIDTLLEQKIITSSPILDQV
                     AAISVGIYQGIPIIDLDYTEDSACDTDMNVVMTGNLGLVEVQGTAEGVTFNRGELNTM
                     LDMAQQGIQELIGIQKSALALS"
     gene            complement(8003..8836)
                     /gene="map"
                     /locus_tag="PK522_01605"
     CDS             complement(8003..8836)
                     /gene="map"
                     /locus_tag="PK522_01605"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNC40461.1"
                     /translation="MQSTMEKVVAIQIKTPKQVEIMRIAGRLAAEVLDHITPHVLPGV
                     TTGELDEICHHYMVNVQKTIPAPLNYAPPGYSPFPKSICTSVNHQICHGIPGAKKLKN
                     GDIVNVDVTVIYDGYHGDTSRMYYVGEPSIQARRLCEVTYESMWRGIEEIKPGKYLGD
                     IGHAIQNMAEKAGYSVVREFCGHGIGAKFHEEPQVLHYGRSGTGIALKAGMVFTVEPM
                     INAGKAAIRHLPDGWTVVTKDGSLSAQWEHTVLVTDTGYELLTVSAGTPPKPTSYQIT
                     T"
     gene            complement(8867..9469)
                     /locus_tag="PK522_01610"
     CDS             complement(8867..9469)
                     /locus_tag="PK522_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40462.1"
                     /translation="MKIFFYLLLFINIITLVIIAIVELNSNKNNRVMEFHAEKIRILP
                     AQVACLEWLSLYGVALQQARTELPKLNLDHFVIESPMDEITVYIIHIAALKTKREVDR
                     QLAQLQKLNIAYQLIQENVGYLQKDSIMLGMYQERSAAMRKLEELSSMNVNNAIISER
                     DIEQVKYIFLEPTEDVRIYLQQLVEKFPDSYLKLTQCDRF"
     gene            9585..10229
                     /locus_tag="PK522_01615"
     CDS             9585..10229
                     /locus_tag="PK522_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNC40463.1"
                     /translation="MKVIKNIVIITALIFSNQILADATKTVQGDAEKGKEIASGVCAG
                     CHNADGNSVIPTNPTLAGQHAEYLLKQLKDFKMEEDTPAKRDNPIMSSMVAPLSVDDM
                     QDLAVFYAQQKTQPANNNVDESQLELGKRVYNGGNLENSVPACSSCHSPNGAGIPPLF
                     PKLAGQHADYTLSQLETFRQGTRANDINNTMQMIVLRLSAQEKKAVAEYISTLK"
     gene            complement(10328..11608)
                     /gene="hemL"
                     /locus_tag="PK522_01620"
     CDS             complement(10328..11608)
                     /gene="hemL"
                     /locus_tag="PK522_01620"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNC40464.1"
                     /translation="MNISNQQLYDLSQQYIPGGVNSPVRAFKSVGGTPIFFQRGEGAY
                     FWDVEGKCYIDYVGSWGPLILGHAHPQVVKAVQETAINGLSFGAPTKAELDLAELLCK
                     LLPSLEQVRLVSSGTEAGMSVIRLARGYTGRSHIIKFEGCYHGHDDALLVKAGSGALT
                     FGHPSSAGVPAETAAHTLVLNFNDVEGLKRTFTELGEQIAAVIVEPVAGNMNLVKATP
                     AFLSELRSLCSRYGSVLILDEVMTGFRVGLTCAQGMYQIQPDLTVLGKVIGGGMPLAA
                     FGGKREIMQCLAPVGGVYQAGTLSGNPVSVAAGLATLREIQAEGFYSTLAVRTTQLTD
                     GLTAMARKHNVAFCAQAVGGMFGLYFSSKLPQSFSEVMACDKEAFNRFFHAMLKEGIY
                     FAPSAFEAGFVSSAHSEDDLSKTLAAADRVFSSW"
     gene            complement(11645..12301)
                     /gene="thiE"
                     /locus_tag="PK522_01625"
     CDS             complement(11645..12301)
                     /gene="thiE"
                     /locus_tag="PK522_01625"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNC40465.1"
                     /translation="MKVNQTRSKTPMVQRSRIEGLYAITPDIENTSDLVEKVDQVLSS
                     GIQYIQYRNKSIAHEQRVNQACAIKKRCQRYGAKLIINDHLDIALAVNADGLHVGKVD
                     VSIQTARTYLGKHKMIGVSCYNQVDLALAAEKEGADYVAFGAFYASKTKIDTVEANPD
                     LLIQAKKVLQIPIVAIGGINVTNAALLIFRGCDAIAVSDGLFNTANIPSIAKKFKRLF
                     "
     gene            complement(12276..13103)
                     /locus_tag="PK522_01630"
     CDS             complement(12276..13103)
                     /locus_tag="PK522_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HNC40466.1"
                     /translation="MLQPPPIVLSFAASDPSGGAGIQADILTLASMGCHPLSVVTAIT
                     VQDTVGVESFLPLESEWVADQARIVLEDMPVHAFKIGVLGSVEAIVTIAEVVADYPNI
                     PLVLDPVLASGRGDSFANDEILQAMRDLLFPQTTIITPNSLEARRLMQENEEDPEIVD
                     LSQSAKSFLRLGCQYVLITGTHENTSKVINTLYDKNGVVRVDEWQRLPGSFHGSGCTL
                     SSAISAALAHGMSVSEGVYEAQDYTWNTLKSGFRPGMGQYLPDRFFWASNEGESDQK"
     gene            13156..13344
                     /locus_tag="PK522_01635"
     CDS             13156..13344
                     /locus_tag="PK522_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNC40467.1"
                     /translation="MSTDQNPVYHRYMCLICGYIYDEAEGSPQEGIPPGTRWEDVPIN
                     WTCPECGARKDDFEMVKL"
     gene            13344..13733
                     /gene="gloA"
                     /locus_tag="PK522_01640"
     CDS             13344..13733
                     /gene="gloA"
                     /locus_tag="PK522_01640"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HNC40468.1"
                     /translation="MRILHTMLRVGNLDNSLQFYTQVLGMKLLRQKDYPEGKFTLAFV
                     GYQHESEGAVLELTHNWDTEKYDIGTGYGHIAIEVADAYQACDSVRKLGGRVIREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKNE"
     gene            complement(13795..14511)
                     /locus_tag="PK522_01645"
     CDS             complement(13795..14511)
                     /locus_tag="PK522_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNC40469.1"
                     /translation="MQYGRLFLIPSPLGEGAIDWIIPSHVKQCIANLTYFVVEHPKTA
                     RHFLKQIALQRPIQAITLQTLDKHTPINELDSLLGPIYAGNDMGLLSEAGCPAIADPG
                     ANLVRIAHEKQIQVVPLVGPSSILLALMASGLNGQCFSFHGYLPIDKVSCAKKIIQME
                     SESFKFNQTQIFIETPYRNKKLFELLAMHCQGSTHLCIASNLTLQDEGINTKTIKDWQ
                     TNLPDFEKKPTVFLLHRYQT"
     gene            complement(14594..14977)
                     /gene="apaG"
                     /locus_tag="PK522_01650"
     CDS             complement(14594..14977)
                     /gene="apaG"
                     /locus_tag="PK522_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNC40470.1"
                     /translation="MDNEKKYDIDIVVRTAYLSEQSDEEAGRHVFAYTITISNTGSIA
                     SQLISRHWIITSGDGNVHEVRGLGVVGEQPLLKPGDSFEYTSGTAISAFAGSMKGSYQ
                     MVAEDGVHFSVEIPEFILSVPRVLH"
     gene            complement(15016..15429)
                     /locus_tag="PK522_01655"
     CDS             complement(15016..15429)
                     /locus_tag="PK522_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC40471.1"
                     /translation="MTEATSGNDLVGKKIMIVDDSNTIRRSAEIFLAQSGCEVILATD
                     GFDAMAKIIDHQPDVIFLDIVMPRLDGYQACMLIKKNPRFHSVPVIMLSSKSGLFDRA
                     RGRMVGSDEYLTKPFTKEGLLNTIKKHTQQQNKVA"
     gene            complement(15550..17007)
                     /locus_tag="PK522_01660"
     CDS             complement(15550..17007)
                     /locus_tag="PK522_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNC40472.1"
                     /translation="MSHFLSVANLLGKIIAVFALTMLIPYGIAIWSDDGSSTIFSDSI
                     LVTFISGIVISLITLPYKKELQIRDGFLLVVLVWLLLPCFGMLPLLNYFPELSIAKAY
                     YEATSGLTATGGTVLTGLDTLPYSINLWRGEMVWLGGMGLIVLAVAILPMLGVGGRQL
                     FKAEIPGPMKEAKLTPRIAETAKGLWLVYLVLTIACLIAYKLAGMNWFDAIMHAFTTL
                     GLGGFSSHDASYGYWNSPLIEFVAMVFMLISGINYATHFLVWRTNSLSSYQNDPEIRP
                     YIYIVLSSCIFLAVYLWLMGVYDDPLHALRYAAFNTISVATTTGYSNTDYSLWPIFAP
                     LWMLFLSGFCTSSGSTGGGIKMIRVRILYRQFFREMITIMHPQAISPVKLGRNIISNQ
                     IIFAVLVFLFVYLSSIILITLLLTLSGLDEMTAFSAAVASFNNLGPGLNQIGPATTYA
                     SLSDFQIWICSFSMLLGRLEFYTLLIVFTAAFWRK"
     gene            complement(17021..18466)
                     /gene="trkA"
                     /locus_tag="PK522_01665"
     CDS             complement(17021..18466)
                     /gene="trkA"
                     /locus_tag="PK522_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNC40473.1"
                     /translation="MKILILGAGRVGSTVAESLVSESNDITVVDIVSGQLALLQDRLD
                     LRVVVGNASYPSVLKRAGAEDADMILAVTESDEINLVSCKLAASLFNTPTKIARIRSA
                     EYLEHSEIFAKDNFCVDFAICPEQIVTEYIENLIEFPEALQVLDFAHGKISLVAVKAL
                     QGGPLVGQQLQELRRHVPNVDTRVAAIFRKDRPILPEGDTIIEVGDEVFFIAATEDIR
                     AVMAELRSMDKPAKRIMIAGGGKIGKRLAYVLEKNYQIRVIERDVKNCEYLAQELQDT
                     LVLQGDVTDEELLESENVAEMDVFCALTNDDENNIMASLLAKKMGARKVIALINRSIY
                     VDLVQSSGIDIAISPAQVTIGSLLAYVRQGDVAVVHSLRRGAAEALELIAHGDANSSH
                     VVGKKIEEVQLPKGATIGAIVRGLPPGEERWENKAGDKENDSGSRNKAKVIIAHHDTV
                     IESEDHVIVFAINKKMIRQIEKLFQVSASFL"
     gene            complement(18528..19322)
                     /locus_tag="PK522_01670"
     CDS             complement(18528..19322)
                     /locus_tag="PK522_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNC40474.1"
                     /translation="MLEQVITIVKRVAEKDVMPRFMQVSRQHKGDGSFYTEADLAAQA
                     SLVTELTKLYPVVVMGEEMSQQQQLQCWEDGAAGLWSIDPIDGTSNFYNGIPYFAISV
                     ALMQNGRGLLGVIYNPATKELFYAERGKGAFLNGVALPIRKSTASLQSAVANVDLKRL
                     SRKLAAKVAANPPYASQRNYGACALEWCYTAAGYFDLYLHGGQKPWDYAAGSLILEEA
                     GGHMCGFEHDDYWLQTPWQRSVIAALDLQLFEQWRDWVREQYLLLK"
     gene            complement(19315..19881)
                     /locus_tag="PK522_01675"
     CDS             complement(19315..19881)
                     /locus_tag="PK522_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNC40475.1"
                     /translation="MASLETPVCEFGWKAVDFDLLGVDGKRYSLSSSRGRSGLLVMFI
                     CNHCPYVKAIRDRIIRDIKELEAYGINSIAIMSNDPLDYPEDSFENMAKVAKEYNYPF
                     PYVWDESQEIAKRYGAVCTPDFFGFNDKLELQYRGRFDASRKEAAPLDAHRDLFEAMV
                     QVAKTGKGPMDQIPSIGCSIKWRTDGNA"
     gene            19929..20729
                     /locus_tag="PK522_01680"
     CDS             19929..20729
                     /locus_tag="PK522_01680"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNC40476.1"
                     /translation="MDHDQPVFTHINPLTMPPRFYCNHPLTINETILLPSGVSHHAAH
                     VLRLKKNNQVILFNGQGGEFTGQIEQIDKNGVTVQIQQFHDINRESPVMTELVQAICT
                     NEKMDWIIQKAVEVGVNRIQPIMTNRCVVHLHGEKVKKRAEHWQQIIHSACEQCGKNK
                     IPELLPLVSYTDWLSQFKNNQADRSITSSTKLMLTPTAQIKLSELQPNNKDFFILLVG
                     PEGGLTLSEEKLAITTGFILIRLGQRTLRTESAGLAALASIQALWGDY"
     gene            complement(20767..21243)
                     /locus_tag="PK522_01685"
     CDS             complement(20767..21243)
                     /locus_tag="PK522_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HNC40477.1"
                     /translation="MNSIYRHCPNSFAKRHFLCSGFTLIELMIAVAVLGIIAAVALPS
                     YQDHVRRTNRAEAKSILLEMSQLLERNYTAVNRYDQKSDGTGFTLSTTQSPKTGTAKY
                     TIQFAAGSLTQNAYTLQAVPTGSMTGDACSTLTLTNTGVKGVGAGATLDAATCWQR"
     gene            complement(21311..24697)
                     /locus_tag="PK522_01690"
     CDS             complement(21311..24697)
                     /locus_tag="PK522_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HNC40478.1"
                     /translation="MKNQVFTGFIIVLLISLLIPYRAHATLALSDVPLFLTTAVDSNI
                     MFTLDDSGSMQFEIMPEEKIYGGVVYMFPRAESIYSGNVYSNASVDFDSNNPYAVALR
                     SSYVNKIYYDPAIRYQPWSNADGSLMSNASITCAPHNPTNTGVGCRDLTTNNTETASW
                     LNSDASLSTSSKTFYPAVYYLYQSGAILSTTSYTKVEIKSSIATYTGSVDRTDCASAP
                     TCSYAEEIQNFANWYTYYRSRILLSRAGIGKAFAAQGSNMRVGFGAINKGNTSIDGVM
                     AYTIVRGVRPFTSSNKSTFFSDLYNHPIYTYGTPLRWAIDDVGRYFQRTDDKGPWGET
                     PGSSGGTQYVCRQNYHILMTDGYWNDGFSGVGNADNTEGSSITNDTPNAAPATYKYTP
                     TLPYSDSFSDTLADVAMYYWKTDLRTDLANKVPTNGKDPAFWQHMVNFTVGLGVSGTL
                     TELPSGAGSWPDPTTSNGAKIDDLWHAAVNSRGEFFSAQNPTAFATGLSQVLNNISAR
                     TSSAAAVTTNTSRLNSGTRIYQAKFNSSDWSGQLLSYNLNSDGSIGTVQWEASAVIPN
                     HSSRKIFTHNGTNGIAFTTSNFSLLTSSQQNALNTNIGGVNDGQGANRVAWLRGDKST
                     ELAQGGSFRNRGAGILGDIINSDLFFVRSLNFSYDGLVSGTPGQTTYYNYVQANESRT
                     PVIYTGANDGMLHAFNADTGVELFSYVPSSVYSKLSSLTSPSYTHRYIVDGNAYAGDA
                     YIGATPSWRTILLGTTGGGGKGIFALDITDPTNFSASNVLWEFTDADLGYVHGQAKIA
                     ILNNGDWVAIISNGYNSTSDKAFLYIVNLQTGALIKKIATNSTTSNGLSAPALVDTNG
                     DKMIDYVYAGDLQGNVWKFDISHTNTSQWDVAYGSGGTKYPLFTARNSSNQVQPITSP
                     LEIGVHPNGGFFIYFGTGQFIASGDNTNTQVQTLYGIWDNGTRISETNRSTLQQQTIE
                     TQTTSSPFRRTVSTNAVNWASKRGWYMDLLKPPSSSNQGERSIVMPLLNFGRILFTTL
                     IPSSDPCASGGSNWVMLLNGETGGMLTAPHFDTDKNGLLNSSDQIIAGVGSDGVRSES
                     VAISAGNLLHLIAGTTTGSVETITVSGNPLTPRRSWRQIQ"
     gene            complement(24716..25210)
                     /locus_tag="PK522_01695"
     CDS             complement(24716..25210)
                     /locus_tag="PK522_01695"
                     /inference="COORDINATES: protein motif:HMM:NF025061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HNC40479.1"
                     /translation="MTNQKGVVLITGLVFLVMMTILGVTAMQNTVLEERMAGNLRDEN
                     LAFQAAEAALREGERFLEQITLPTFNGSDGLYHHASSPAPDPMTWTGWTTSGKAISTS
                     ITGAASQPRYIIEQLVSVPFQGNKGSAQQSPVTNMSNMYRVIARGVGGTDSAVVVLQS
                     IYRR"
     gene            complement(25256..26293)
                     /locus_tag="PK522_01700"
     CDS             complement(25256..26293)
                     /locus_tag="PK522_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HNC40480.1"
                     /translation="MKKVINQQYGFTLIELMVAMTIGLLLLGGVITILSSSHQTYRVN
                     DALSRLQENARYAFQLLSRDIRMAGYLGCVGDGVPIVNTLNNPSDFLWKLGQPIEGFE
                     ASSSSAWTPALPSPSGTIPSPLGGRDIVVIRGVDGTDVKVIQHPGGNPPGSADLKVTV
                     GSGLQEDDIVLVTDCLAAAVFQVTNVNTSAGQDNVVHNTGNPTANTPGNLTKALGKEF
                     TGGELIKISTRSYFIRMNSNPTPFPSLYRKVGAGNAEELVEGVEDMQIEYGEDLDGNS
                     TADVYRTADAVTNWAEVVSVRISLLMQSLENNVATDAQTYTFNGTITTPNDRRLRQVF
                     TTVVTLRNRTS"
     gene            complement(26301..26744)
                     /gene="pilV"
                     /locus_tag="PK522_01705"
     CDS             complement(26301..26744)
                     /gene="pilV"
                     /locus_tag="PK522_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HNC40481.1"
                     /translation="MLSTNIKNQIATQKGVTMIEVLVSVIVLSIGLLGLAGLQSAGLT
                     HNQSASFRSTATMMTYSILDSMRANRTEASNGNYNIALGAAKPTGDATYQKDLNNWMT
                     ELALRLPTGLGSVNVDNNNNVTITIQWDDSRGSMAAQQFMMATRL"
     gene            complement(26738..27259)
                     /locus_tag="PK522_01710"
     CDS             complement(26738..27259)
                     /locus_tag="PK522_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HNC40482.1"
                     /translation="MHLIVNGGFTTLELMITLSILAILASVAVPSYQGMMVQSRLTTQ
                     TNELVTTLHFTRSEAIKRGSRVTICKSNNGASCTTASNWHDGWIVFTDTGVSGTVDGA
                     DQVLRVFPALKGSTLMSGGNFQNWLSYMPNGRALSGNFQNDTFRFCASAKRRDVVING
                     TGRPQIDKSVTTC"
     gene            27371..28408
                     /gene="thiO"
                     /locus_tag="PK522_01715"
     CDS             27371..28408
                     /gene="thiO"
                     /locus_tag="PK522_01715"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HNC40483.1"
                     /translation="MSQFLLIGAGIIGLTSAVHLLKEGFSVTVLEKGEVGQESSWAGG
                     GILSPLAPWDYSEPVTQLVGYSTSIYSKWVDELSLSTEIDPEFNECGMLVLPPYDSVK
                     AHQWCISHNIKEEINHKSFWSPIHQSALEEAIFLPRVAQIRNPRLLAALQCEVIKRGG
                     VIKEFCEVSRLNLKNKSVVSISTTSGNYSADGYIITAGAWSRKILGKYALNLNIIPIQ
                     GQMLLYKFDAPPIPSILLQKELYLIPRLDGHLLIGSTMENVGFNKQITQAAYDKLYKK
                     ASRLLPEIETKHLIKHWAGLRPASPSNIPTIGRHPDLHNLFINSGHFRYGVTMAPGSA
                     EILLNEIMGKP"
BASE COUNT         8715 a         6594 c         5092 g         8139 t
ORIGIN      
        1 ccatcgtaca gccagaataa ggtgcaataa attgcatcgc tgctgattct gacgcagaag
       61 caacgacaac tatcgtatac gccattgcgc catgttcttc caattttctg actacgttcg
      121 caattgatga agctttttgg ccaattgcta catagatgca gatcatgttt tcaccttttt
      181 ggttaatgat cgcatcaatt gcaacagcag tttttccagt ttggcggtca ccaatgatca
      241 attcacgctg ccctcttcca accggaacca tggcatcaat cgcttttaat cccgtttgca
      301 ctggctgatc aactgatttt ctccagataa cgcccggtgc aatcttctca atgggttctt
      361 tcccttgcgc agaaattggt cccttcccat cgatcggctg acccaatgca ttaacaacgc
      421 gcccaagcaa tccttcacca acaggaactt ctaaaatacg gccggtacat ttaaccgtat
      481 ctccttctgt taaatgctca taggaaccta aaataaccgc accaaccgaa tctctttcca
      541 agttcagcgc taagccatag gtattccctg gaaactccag catttcacct tgcataacat
      601 ctgaaagtcc atggatacga acaattccat ctgttagcga tacaatggtt ccctgtgtgc
      661 gtatttctga cgacgctgat agtccttcta tcttactttt aatcagctca ctaatttctg
      721 atggattcaa ctgcatttca ttaactccta gctttttagg gcaatagaca tagcttctag
      781 tttaccggat atcgagctat caataacctc gtcgccaatc tcgattttta ccccaccgat
      841 taattcctga tcaatactta ctttcgcttt taccttacga ttgaatttat tctcaagcgc
      901 agctaccaat ttctccagtt gcctatctga cattgaaaag gctgaaatga tattcgcctc
      961 taagacgcct tcatgatttg ctcttaactg ctcatacatc tcaactaacc ggggaatcat
     1021 cgtaacccta tcattgtcaa tcagaatcga tagcaaattc tttacttcgc catcaagttt
     1081 actaccgcct atattgaaaa taatctcctt tagcttcgat gccgaaacgt ttggattacc
     1141 aatcaattct tgaacaggag caacttctgt aatctgcgca gcgttactta acatttccga
     1201 ccaatgcgat aagctatttt tttcctttgc aagcttaaaa acagcttctg catagggacg
     1261 agctatagtt attgactcag ccattacttt aactctgttt caattgctgt taataaatca
     1321 gcatgcgctt ttgcatcaat ctcacgccgc aaaatttttg ttgctccagt taaaacgaga
     1381 ccacttactt gttggcgtag ctcttctttt gcactaaaaa tttcatgcgc tatttctgct
     1441 ttagcacccg caataattcg atcaccctct acttttgcaa ctgattttgc ttcatcaatg
     1501 atttcagccg ctcgtttttc tgcctgagtg acaatatcag ccgcacgttg ctttgcatct
     1561 ttaagaacat cagatgatcg ttgatctgca agttcaagtt cttttcttcc acgctctcca
     1621 gcagcaagtc catccgctat ttgtttttga cgttcttcaa tcgcacgcag tagcggcggc
     1681 cacacaaact taactgtaaa ccaaacaaat acggagaagg caatcgcctg tgaaataagt
     1741 gtaaaattta tattcattgg gcagctttgt taaaattttt cttgtttaaa tactattgaa
     1801 cgacagctaa caaaggatta ccaaaagcaa atagcattgc caaaccaact gcaataatga
     1861 atgaagcatc ggtcagcccc aataacaaga atactttacc ttgcaaagtt ggaatcattt
     1921 caggttgacg cgccgctccc tctaaaaaac gactacacat cacacccaca ccaatacacg
     1981 ctcccgccgc acccaatcca atcattaacc caatgccaat tccggtgtaa gcttgaatca
     2041 tcgcgagata ctgcaaattt tccatatcat ttccttttta tttaatgaat aaaactaaat
     2101 ttctaactaa aacaacaacc ttaatgggac tcatgcgcca ttgcaagata aacaacggtt
     2161 agcatcataa atataaaggc ctggagacta acgattaaaa tatgaaaaat cgcccaacca
     2221 gcccctaaaa ttgcactaaa gaatgcgcct gatacaccag tagccgccca catccctaac
     2281 aataagaaaa taatttcacc agcatagata tttccaaata atcgcaatga atgagaaagc
     2341 ggcttagata tatactcaat catgttaaag agtaaattta gtggccataa gatgatattt
     2401 gtaccaaagg gcgtgcagaa caattcatgc atccaaccac ctaacccttt tactttaatg
     2461 ctaaaaaata tcgtcaacag ccatatcgat aacgctagcg caaatgttgt attaacatca
     2521 gttgtaggca cactacgcca attatgtaag ccaaacacat gctcataaac ccaagccatt
     2581 atgtcaatgg gcaacaaatc catcgcatta agtagcaaaa cccaaacaaa aattgtcagg
     2641 gctgccggcg ctataaatac atgtctattt ccgtggaaag tatttttaac ctgattatca
     2701 ataaactcga ccaataactc aacaaaagct tgcgttttac tgggcacacc cggcgtcgcc
     2761 ttacgcacaa tcaaccaaag aaaacccata cccattatgc ccaacatcac cgacataact
     2821 agcgtatcaa gatgcagcgt ccaaaatgaa ccatcaccca cagtggtggt catatttgtt
     2881 agatggtgat taatataaga agttggtgta agttctgctg ccattatttt tagcttttta
     2941 ttcccattat ttattttttg cagttgttac aaatagagcc atactgaaga taattgccgc
     3001 gacaatgaat accccaataa acacaattgg aacaacttct ttatataaca aaaatactcc
     3061 caaaaggagt acgatcatta tcgaaatctt aatcgcttct gctttaagcg ccgtcttcac
     3121 tacatcaccg gctgtatatc ctccatgacg atcgatcaat acagcatatg ccaatgaagc
     3181 agcaatactg actaaaccgc ccagtaaagc agaaattgcg ccatgcattc ccattaacca
     3241 tccacaaaca agagttgcca aacccgttgc tagcgtttgc caaaatacaa ccctacgtaa
     3301 tggttcattc cgtatccaag acatataatc tttgccagaa ccagaaagta cgtaaataca
     3361 ctttacttta aagtcccaga ttttaacctg cctggcaatt tacgtcaacg aagcaaatga
     3421 ctttgactcg acaaatgccc tcgaaatata ctaggaataa agatgtagaa aacataaata
     3481 ataatctcaa gatttaaatt aatcatgcta ctaacaacca caccgatgat tgaaggcaaa
     3541 cgtatcacac aatactgcgg tattgtcgcc ggagaagctg tgctcggtgc caatgttctg
     3601 aaagatcttt ttgctggtat tcgtgatttc atcggtggac gatcaggaac ctatgaaaaa
     3661 gaactacagc gcgcccgcga tatcgctctt aatgaattaa aagataaggc taaagaactg
     3721 ggcgcaaacg cagtcatcgg tatcgacata gactatgaag tattaggcaa agaaaatggc
     3781 atgttgatgg tatctgctag tggcacagct gttatcttcg aatgagccag tattaacata
     3841 accgctttct atctatcttt ctcagaaaaa ttcaactttt ttgatgcatt cagtgatatt
     3901 gcgtcaattt attttacaac cagcttacca tcattaaacc aataaacaca taaccctctg
     3961 attaataatc acaataaata actggcatta ttgttgctct ctatagctac taaaaccttc
     4021 ttggcgtaaa gaattcaaca tcccatgttt cttaagtaac cgatggatac agattcatta
     4081 catcagcaag gcaattcatt tttattttta gattaggcta tcgatagaac atgttagtta
     4141 aatacaattt aaaccatcaa gcagtcacat attattggaa gcctatttca cgctaccaac
     4201 aatttcagtt agtcggagaa ttttattatc agtccgcttt taagaagcta gttgaaaatc
     4261 atggcaatag aggggtttat ttcgaatgcc tcgccacgct cattccagaa aacaccaacg
     4321 catttgatag tcaagccatt cgtattgata ttaaggggat gactgttggt tattttaatc
     4381 gtaatgacgc tagaaagttc cgcatacgat taaatactaa taaactcgct gggcaaatta
     4441 caagctgtaa cgcagtgata gaaggtgggc gtctcttaaa tgacgggcgc cgtagtaatt
     4501 atggcgtatc gcttgctatt aaccagcttt agcaaaaaat cagaacgcgt taccttatcc
     4561 ttgtcccctt gttgttatat agagcgcaag gcctgtgaaa accattccac cgagaatatt
     4621 acctaaaatc actggaatac cattccacgt ccaccattcc aggaacgtta tatttgcacc
     4681 caacatcata cccgcaggaa taacaaacat attcacaatg gcatgctcat aacccaacgc
     4741 aaaaaaagtc atggtcggca accacattgc agcaattttt cctcctgttg aggtagatgt
     4801 gaaagcgata actgacccta acgctaccat ccagttacat agcattgcgc tgataaaggc
     4861 tgtagccatt cccccaaaac caattgccgc gtaggcaatt gtcttctgct ctgcgagtgc
     4921 aactaacttt attgcttgat ctagattaat tggatcaaca tgaccgagtt tcgtgatgac
     4981 caaaacaaaa agaaatgcat aacctactcc cccgatcaaa tttccgacta aaacccaaaa
     5041 ccaattatga aacaaacgtc ctatacgagt tcggccatct tttaccgcaa tcgggatcac
     5101 tgcaaaattt cctgttgcta attcgagccc tagtagtacg atcatcacca accctacggg
     5161 aaacacgatt gctccccaaa ttcccaggcc tgtttgaaca ataaccaaaa ccgcaagact
     5221 tgttgcatag cctagaaatg ctccggcaag agcccctcga atcagcatat ctttgatcga
     5281 aagatttgct ttcttggttc ctgcatccaa catacattca acaacttcgg ttaattttac
     5341 ataagtcatt ggatttcaat cacgctttct gagaacgata ctgataagct atttattggg
     5401 caaaaaaatc tgcaatagat catttaaaaa ttttctgcct atatcggtcg gcacgatacg
     5461 atgacaatcc cgatgaatca atcccatctg ctctgcaact ttaagctgct gttgtgcagc
     5521 aataatgggt aatcctgttc gagaacgaaa atcttctaga cgaaatccct cgataaggcg
     5581 tagtgaattc atcataaatt cgaagatacg atcagctact tcgagctcct gctccatttg
     5641 aataggcgta actgattggc tcaacaatac ctcaagatat ttcttggggt gcttatagcg
     5701 catttggcga acgatcttgt cacgaaagct aatcttactg tgcgcgcccg cgcctatccc
     5761 caaataatca ccgaactgcc aatagttcaa gttatgatga gaccgatgtc cagcatgcgc
     5821 aaaagctgag atttcatact gagcaaaccc agaactcttc aaagtttgct cgatcaatgc
     5881 ttgcatgtca gcagacaaat catcatctgg caattgcggt ggatattgat aaaacattgt
     5941 attcggttct aacgttaaat gataagccga aatatggggc acgccagtag tacaagcggt
     6001 ttcgatatct actcgcgctt cttcaatcgt ttgattgggc aatgcgtaca ttaaatctaa
     6061 attgatatta tcaaaataat gctgtgaaat ctcgattgca tggcgggctt cattggcatt
     6121 atggattcta cctaacttta acaactgctc tgtgttaaag ctttgaacgc ctattgaaag
     6181 acgattcacg ccagcttcac ggaatgcttc gaattttttt gattcaaatg tacctggatt
     6241 ggcctctaag gtaatttcaa cggaaggttc caatagcaat agcatcctga cttgagacaa
     6301 tagttgctca agtcctgatg cactcaataa gctaggcgta cctcccccaa taaaaatact
     6361 actaatagat cgcccccaaa tttgaggtaa tgcagcctct aaatcgcgca acagcgcaga
     6421 taaatattct ttctcgggca aatcatttgc cccactccca atttcatgtg aattaaaatc
     6481 acaataaggg cattttttga tacaccaggg aaaatgaatg tacaaactga gcggaggtag
     6541 ctcaagtaag tgaggtgatt gaaaatttaa aactggcgca atcgttttgg gttcaaccat
     6601 aaaaactaga aacgacaatt cacaagcatt tgggtcagtt ttaccagcgc cttcgaacga
     6661 tggctatatt gattcttttg ctctgaagtt aactgcgctg cagtttttcc taattctggt
     6721 acaaagaaat gcggatcata gccaaatcca ccctcacccc ggggctctag gattatttca
     6781 ccaaaccaat agccatcagc gatgattggt tgtggatcat tagcactgcg aattagcacc
     6841 ataatgcagt aataataggc tgctcgatct gaattaaatt tgagtgtctc aataagcttt
     6901 tgattatttc gctgatcgga tttgggctca cctgcataac gagccgaaaa gatacccggc
     6961 gcccctccta atgattgcac acaaatccct gaatcatctg caagtgcagg caatcctgtt
     7021 tgttcactcg catggcgtgc ttttgctagt gcattttcaa caaatgtttg ataaggctcc
     7081 tcgatttcag tcacacccaa atctttctgt ggcagtattt gaatttcaag tggcaccaag
     7141 atttgttcaa tttctcgtaa tttacccgtg ttattacttg caatgattaa ttgctgcata
     7201 cctttcctaa ctgagcgcca gcgctgattt ttgtatgcca atcagctctt gaattccttg
     7261 ttgggccata tcgagcatcg tattcaattc accacgatta aaagttaccc cttctgcagt
     7321 tccttgtact tctactagac caagattccc tgtcataacc acattcatat cggtatcaca
     7381 agccgagtcc tccgtgtaat caagatcaat gattggaata ccctgataaa tccccaccga
     7441 aatagctgcc acttgatcga gaatagggct actggtgata attttctgtt ctagcaatgt
     7501 atcgatagcg tcatgtaaag caacataagc accggtaatg ctcgctgtac gagttccacc
     7561 atctgcctga ataacatcac aatcaatctg tatggtacgt tcaccgatta aggctaggtt
     7621 agtaatcgcc cgaagtgaac gaccaatcaa tcgttgaatc tccatggtcc gtccagattg
     7681 cttgcccttt gaagcctctc gctgaattcg ctcgtgagtg gaacagggaa gcattccata
     7741 ctctgcagtt agccaacctt gaccacttcc tttaagaaac ggaggaacct tatcagagat
     7801 actcgcggta caaataactt tcgtatcacc gcattcaatt agtactgatc cttgagcata
     7861 tttcgtatag cgtcgcgtta ctgatatggg acgaatttga tacgtagctc gattattctt
     7921 acgctgcatg agttatcatt attagaattt agcaatcctc ttttaacaag aggaaatgaa
     7981 ggtggattct acattaaaac cactaagtgg ttatttgata ggaagttggt ttaggtggag
     8041 tccctgctga aaccgttaac aattcatagc ctgtatcggt cactaacacc gtatgctccc
     8101 actgtgctga caaactaccg tcttttgtca ctacggtcca accatcaggc aaatggcgaa
     8161 ttgcagcttt tccagcattg atcatcggtt ccaccgtaaa taccattccc gctttaagag
     8221 caatccctgt ccctgatcgg ccataatgaa gcacttgagg ttcttcatga aatttagcac
     8281 cgatgccatg gccacaaaat tctctgacaa cactataacc agccttttca gccatatttt
     8341 gaattgcatg gccaatatca cccaggtact ttccaggttt tatttcttcg attccccgcc
     8401 acatggattc ataagttact tcacataatc gcctagcctg tatggaaggc tccccaacat
     8461 aatacatacg actcgtatcg ccatgataac cgtcataaat tactgtcaca tccacattaa
     8521 cgatatcccc attcttaagt ttcttggcgc ccggaatacc atgacagatt tgatgattga
     8581 ctgacgtaca aatcgatttt ggaaaagggc tatacccagg cggtgcgtaa ttgaggggag
     8641 ctgggatagt tttttgcaca ttaaccatat aatgatggca aatctcatct agttcacctg
     8701 tcgtaactcc tggcaataca tgtggtgtaa tatggtccag tacttcagca gccaacctac
     8761 ccgcaatccg cattatttct acttgctttg gcgtttttat ttgaatggcc acaactttct
     8821 ccatagttga ctgcaatttt ttgttttttt catttctgtt gtatttctag aatcgatcgc
     8881 attgagttaa tttaagatag ctatcaggaa atttttcaac aagctgctga aggtaaattc
     8941 tcacatcttc cgttggttct aaaaatatgt attttacttg ctctatatca cgctccgaaa
     9001 taatggcgtt attcacattc atactactta actcctcaag ctttctcatt gcagccgatc
     9061 gctcctgata cattccaagc ataatcgagt ctttctgaag gtagccaaca ttttcttgaa
     9121 tgagttgata agcaatattt agtttttgca attgggcgag ttgtctatcc acttcccgtt
     9181 tggtttttag tgctgctata tgaatgatgt aaactgtaat ttcgtccata ggcgattcga
     9241 taacaaaatg atctagatta agtttgggaa gctcagttct agcttgttga agtgccacac
     9301 cgtataaact tagccactcc aaacacgcaa cttgtgcagg taaaatccta attttctcag
     9361 catgaaactc cataactcta ttatttttat tcgaattcaa ttcgactatc gcaataatta
     9421 ccaacgtgat gatgttgata aacaataata agtaaaaaaa tattttcata tataattgaa
     9481 ttattaccta ttaacgttta ctgttgttag caaaagttgg tagaattcgt cgttaatcgt
     9541 agtgtttttt tattgaaacc ttaaaaaaca acaatttctc aaaaatgaaa gttattaaaa
     9601 atattgtaat cattaccgcg ttgatatttt ctaatcaaat tctggcagat gctaccaaaa
     9661 ctgtacaagg tgatgccgaa aagggtaaag aaatcgcttc aggtgtttgt gcgggttgcc
     9721 acaatgcgga tggcaatagt gttatcccaa caaatccaac gcttgcagga cagcacgctg
     9781 aatatcttct gaaacaatta aaagatttta aaatggaaga ggatacacca gccaaacgag
     9841 ataatccaat catgtcatcg atggtcgcgc cgctcagcgt agatgatatg caggatttag
     9901 ccgttttcta tgcccagcaa aaaacacagc ctgctaacaa taacgtcgat gaatctcaat
     9961 tagaattggg gaaacgagta tataacggtg gcaatctaga aaattcagtg ccagcgtgct
    10021 caagctgtca ctcaccgaat ggagcaggta taccaccact cttccccaag ttggctggac
    10081 agcatgcgga ctatacctta tctcagctag aaactttccg gcaaggtacg agagcaaacg
    10141 atatcaataa tacgatgcaa atgatcgtat tacgtctgag cgcgcaagag aaaaaagctg
    10201 tcgctgaata tatttcaact ttgaaataac taatcagcac tgcttactca agcttcatac
    10261 aaaaaagcct gcctcaaaat tttgaagcag gcttttttta tttatttctc aataattgat
    10321 taatttccta ccaggaacta aatactcgat ctgcggcagc taacgtttta gatagatcat
    10381 cctcagaatg cgctgaagaa acaaatccag cttcaaatgc cgaaggcgca aagtaaatac
    10441 cttcctttag catcgcatga aagaaacgat taaatgcttc tttatcacaa gccattacct
    10501 ctgaaaaact ttgaggaagc ttactgctaa aatataaacc aaacatgccg cccacagctt
    10561 gcgcacaaaa tgccacgtta tgtttccttg ccatagctgt taagccatct gttaactggg
    10621 ttgttctgac ggcaagtgtt gaataaaaac cttctgcctg aatttctcgt agtgttgcta
    10681 agcccgctgc aacagaaaca ggatttcctg aaagggtgcc tgcttgataa acgccaccga
    10741 caggcgctag gcattgcatg atctctcgtt tcccgccaaa ggctgcgagg ggcatccccc
    10801 ccccgatcac tttacctaaa actgtcaaat cgggttgaat ttggtacatc ccttgtgcac
    10861 aggttaaacc aacacgaaag cctgtcatta cttcatcaag aatcagaaca ctaccataac
    10921 gagaacataa gcttctcagc tctgataaga aagctggcgt tgccttaact aagttcatgt
    10981 taccggctac cggctcaacg ataaccgctg caatttgctc acccaattca gtgaaagtac
    11041 gctttaatcc ttctacatca ttaaaattaa gcacaagcgt atgtgctgcc gtctcagcag
    11101 gaacacctgc cgagcttggg tggccaaaag tcaatgcacc agaacctgct ttaactaaca
    11161 atgcatcatc gtgaccatga tagcagcctt caaattttat gatatgactt ctcccggtat
    11221 acccacgcgc taaccgaata acgctcatcc cggcttctgt tcctgagcta accagacgca
    11281 cttgttctaa cgatggtagt aacttacaaa gtagttcggc cagatccagt tctgcttttg
    11341 tcggagcacc aaaagatagc ccattaattg cggtttcttg aactgcttta acaacctgag
    11401 gatgtgcatg tcccaatatt aaaggtcccc aggaaccgac ataatcaata taacatttac
    11461 cttcaacatc ccagaaataa gcaccctccc cccgttgaaa aaaaatgggc gtccccccta
    11521 ctgatttaaa cgctcttacc ggtgaattaa cgcctccagg tatatattgc tgggaaagat
    11581 catataactg ctgattactt atattcattt ggtttctcaa ttaattaaaa aaatatgaat
    11641 ttgctcaaaa taaacgttta aatttttttg cgatagaggg aatattagca gtattaaata
    11701 aaccatcact tacagcaatg gcatcacagc cacggaaaat aagcaaagcc gcattcgtta
    11761 cattaatacc cccaattgcc acaataggga tttgtaaaac tttcttcgct tgaatcagta
    11821 aatcggggtt cgcttcaacg gtatcgattt tggtttttga agcataaaac gcaccgaaag
    11881 caacataatc ggcgccttct ttctcagcag ctagcgctaa atcgacctga ttgtagcaag
    11941 aaacccctat cattttatgc ttacccagat aagttcttgc tgtctggata gaaacatcaa
    12001 ctttcccaac atgcaaccca tcagcattga cggcgagcgc gatatccaaa tgatcattga
    12061 taattaattt agcgccatat cgctgacacc gcttcttaat cgcacaggct tgattcactc
    12121 gttgctcatg agcaatagat ttatttcgat attggatgta ctgaataccg gatgatagta
    12181 cttgatctac tttttcaacc aaatcgcttg tattctcaat atccggagta atcgcataca
    12241 atccttcgat tctagatcgc tgcaccatcg gtgtcttact tctggtctga ttcaccttca
    12301 ttggaagccc aaaaaaaccg atcaggcaaa tattgtccca tccctgggcg aaatcctgat
    12361 tttagcgtgt tccaagtata atcctgcgct tcatatacac cttctgatac tgacattcca
    12421 tgcgccaatg cagccgatat cgctgaagac aaagtacaac ctgatccgtg gaatgatcca
    12481 ggcaaccttt gccactcatc aactcgaaca acaccattct tatcataaag cgtattaatt
    12541 actttagaag tgttttcatg tgtacctgta atcaagacat attgacagcc taaacgcaaa
    12601 aaagattttg cactttgact caggtcgact atttcaggat cttcttcatt ctcctgcatt
    12661 aaccttcttg cttccaaact attaggtgta atgattgtcg tttgtggaaa aagtaaatcg
    12721 cgcatcgctt gaagaatttc gtcattagca aatgaatcgc cacgacccga tgcaagcacc
    12781 ggatcaagca caaggggtat attaggataa tccgcaacca cttcagcaat ggtcacgatt
    12841 gcttcgacac tccctagcac accgatttta aatgcatgaa ccggcatatc ctcaagcacg
    12901 attcgcgctt gatctgcgac ccattccgac tcaagcggca ggaaactttc cacaccaact
    12961 gtatcttgga ccgtaattgc agtaactaca gaaaggggat gacaacccat actcgctaat
    13021 gtaagaatat cagcttgaat gcctgcacca ccactcggat cacttgcagc aaaggatagt
    13081 acgataggtg gtggttgtaa catgcaataa tcccgtaaaa tagtatttta acttaaaagg
    13141 atatatcaat ctattatgtc aaccgatcaa aatccagttt atcaccgtta catgtgcctg
    13201 atatgcggat atatttacga tgaagcagaa ggttccccac aagagggcat tcccccgggt
    13261 acacgttggg aagatgtgcc catcaactgg acatgtccag aatgcggtgc tcgtaaagat
    13321 gattttgaaa tggttaaact ttaatgcgca tacttcatac gatgcttcga gttggaaatc
    13381 tcgacaactc gcttcagttt tatactcaag tcctcgggat gaaattgctc cgtcagaaag
    13441 attatccgga aggaaaattt accctagctt tcgttggtta tcagcatgaa tcagaaggtg
    13501 cagttctcga gctcactcac aattgggata cagaaaaata tgatatcggg accggctatg
    13561 gacacattgc gatagaagta gcggatgcat atcaagcctg cgatagcgtt agaaaattag
    13621 gtggtagagt cattcgtgaa gcaggcccca tgaaacatgg aacgacagtc atcgcatttg
    13681 ttgaggatcc tgatggttat aagattgaat tcattcaaaa gaaaaatgaa taagcaaaag
    13741 gactttgtat gacaaagtag caagaaatta ctcaaaaaac tctccacctt gctcttaggt
    13801 ttgataccga tgtagtagaa aaactgttgg ttttttttca aaatctggca aattagtttg
    13861 ccagtctttt attgtttttg tgttaattcc ttcatcttgc aacgtaagat tgctcgctat
    13921 acataagtgc gtgcttcctt ggcaatgcat agccagcaat tcgaataact tcttatttcg
    13981 ataaggtgtt tcgataaaaa tctgagtttg attaaatttg aatgattccg actccatctg
    14041 aattatcttc ttggcacacg ataccttatc gattggaagg tatccgtgga aggaaaaaca
    14101 ctgtccgttt agcccagaag ccattagtgc caacaatatc gaagatggcc ccactagagg
    14161 aacaacttgt atttgctttt catgagctat tcggactaga tttgcaccag gatcagcaat
    14221 agccgggcat cctgcctctg atagcaaacc catatcattt cctgcataaa taggcccaag
    14281 caagctgtct aattcattaa taggtgtatg tttatccagg gtttgtagcg tgatagcttg
    14341 aatcggtcgt tgcaaagcaa tttgctttag aaaatgtctc gcggttttcg gatgctctac
    14401 aacaaagtac gttaaattcg caatacactg cttaacatgg cttggaatga tccaatcaat
    14461 ggctccttca cctaagggag aaggaatgag aaatagccta ccatattgca tacaagggta
    14521 agaatataac gcggctactt aataatgctc ggtagcgagt agcatcaatt aactttatag
    14581 cttaagacaa aaatcaatgt agaactctcg ggacacttaa aataaattca ggtatttcta
    14641 ctgaaaaatg aacaccatcc tcagcgacca tttgatagct tcctttcata gagccagcga
    14701 atgcagatat cgctgtgcca cttgtatact caaaactatc accaggcttt agcaagggtt
    14761 gttcaccgac gacacccaac ccccgcactt catgtacatt tccatcacca ctcgtaataa
    14821 tccaatgtcg gcttatcagc tgcgaagcaa ttgaacccgt attggaaata gtaattgtgt
    14881 aagcaaacac atggcgaccc gcctcctcat ctgattgctc cgatagataa gcagttctaa
    14941 ccactatatc gatatcatat ttcttttcat tatccatgtt ctaaccacta taaccataca
    15001 aaataggtta ttttatcaag caactttatt ttgttgctgc gtgtgttttt ttatcgtgtt
    15061 gagtaaacct tctttggtaa aaggcttagt taaatattca tctgatccga ccattcttcc
    15121 acgagcacga tcaaataagc cacttttact tgataacata attaccggaa cagaatgaaa
    15181 gcgggggttc ttcttgataa gcatgcaagc ttgatagcca tccaggcgag gcattacgat
    15241 atcaagaaaa ataacatcag gttgatgatc aatgatttta gccatcgcgt caaaaccatc
    15301 ggtcgctagg ataacttcac aacccgactg agccaggaaa atttcagcac tgcgacggat
    15361 cgtattgctg tcatcaacga tcatgatctt tttccctact aaatcatttc cacttgttgc
    15421 ttccgtcatt tctatgctcc cttttattct cgtctatata tggaaagcac tccatcactt
    15481 ccccttgaac tagcaaacga ttcattaaca caatagctct attatagggc gaaacataac
    15541 gatacagaat cacttccgcc agaaagctgc tgtaaataca atcagtaatg tataaaactc
    15601 cagtcgtccc aataacatag agaagctaca tatccagatt tggaaatctg aaagcgatgc
    15661 gtaagtagtt gccggaccga tctgatttaa tcctggccct aggttattga aactcgcgac
    15721 cgctgctgag aaagcagtca tctcatcaag tccactcagt gtaagcagca acgttattaa
    15781 aatgatcgaa gataaataaa caaataaaaa aacaagcact gcaaaaataa tctgatttga
    15841 gataatgttt cgaccaagct taacaggaga aatagcttga ggatgcataa tcgtaatcat
    15901 ttctcggaaa aattgtcggt acaaaatacg tacccgtatc attttaatac caccacccgt
    15961 tgaccctgac gaagtacaaa agcctgacaa aaacagcatc cataagggtg caaaaatagg
    16021 ccataaacta tagtccgtgt tgctatagcc agtcgtagta gccactgaaa tagtattaaa
    16081 agccgcatag cgaagcgcat gcaaaggatc atcataaacg cccattaacc acaaataaac
    16141 tgcaagaaaa atgcagctcg ataacacgat atagatataa ggtcttattt ctgggtcatt
    16201 ctggtaagaa gaaaggctat ttgttcgcca aactaaaaag tgagttgcat agttaattcc
    16261 actaatgagc atgaatacca tcgccacaaa ctcaattagg ggtgaattcc aatatccata
    16321 gctagcatcg tgagaagaga aaccaccgag gcccagcgtg gtaaatgcgt gcattattgc
    16381 atcgaaccaa ttcattcctg ctagcttgta agcaatcaaa caagcaattg tcagaaccaa
    16441 gtagacaagc cataacccct ttgctgtttc tgcaatcctg ggggtcaatt tagcttcttt
    16501 cataggacca ggtatttctg ctttaaataa ctgcctgccc ccgaccccaa gcataggcaa
    16561 gattgcgaca gctaacacaa tgaggcccat tccacccagc cataccatct cccctctcca
    16621 aagattaatc gaataaggca atgtatccaa tccggtcaaa accgttccac ctgtcgcggt
    16681 tagtcctgaa gttgcctcat aataggcctt tgctatactc aattcaggga aatagttaag
    16741 taaaggaagc ataccaaaac atgggagcaa taaccacact aacacaacca gtaaaaaacc
    16801 gtcccgaatc tgcaattctt tcttataagg aagagtgata aggcttataa caatgccaga
    16861 tatgaatgta accagaattg aatcactaaa aatagtagat gagccatcat ctgaccaaat
    16921 tgcaatccca taggggatta acatggtcaa agcgaacaca gcaatgatct taccgagtaa
    16981 attcgcaacc gataaaaaat gactcattaa atattagtta tcacaaaaat gatgcgctca
    17041 cctgaaacaa tttctcgatt tgtcgaatca tttttttatt aatagcaaat acaatgacat
    17101 gatcctccga ttcaatcacg gtatcatggt gcgctataat cactttggct ttatttctgc
    17161 ttcccgaatc gttttcttta tcaccagctt tattctccca tcgttcttct ccgggaggta
    17221 gtcctcttac aatggcacca atagtagcgc ccttgggtaa ctgaacttcc tctatttttt
    17281 ttccaaccac atgggaggaa ttagcatcac catgcgcaat caactctagc gcttccgccg
    17341 cgccacgccg taagctatgc actactgcaa catcaccttg tctgacataa gcgagcaatg
    17401 aacctatcgt cacttgcgct ggagaaatcg caatatcgat gccactgctt tgaactaaat
    17461 caacatagat actgcgatta atcaaagcaa taactttacg tgcccccatt tttttggcga
    17521 gcaatgaagc cataatattg ttttcatcat cattagtcag tgcgcagaac acatccattt
    17581 cagctacatt ttcactttcc aacaattcct catcagttac atcaccctgc aacactaatg
    17641 tatcttgaag ttcttgcgcc aaatactcac aattcttgac atctcgctca atcactctaa
    17701 tttgatagtt tttttcaagt acatatgcaa gacgtttacc gattttaccg ccgccagcaa
    17761 tcatgattcg ttttgctggc ttatccatac tacgtagttc tgccattaca gccctaatat
    17821 cttcagtcgc agcaataaaa aagacctcat caccgacttc aatgatcgta tcaccttctg
    17881 gaagtatggg ccgatctttc ctgaatatag cagcaacacg tgtatcgaca ttgggtacat
    17941 gacggcgcaa ctcttgcaac tgctgaccca ctaatggtcc cccttgcagc gcctttaccg
    18001 ctactaaact tatttttcca tgagcgaaat ccaatacctg cagcgcttct ggaaattcga
    18061 ttagattctc aatatattct gtaactatct gttcagggca aatagcaaaa tctacacaga
    18121 aattatcttt tgcaaaaatt tcagaatgct caagatattc ggctgagcgg attcgggcaa
    18181 tttttgtggg tgtattgaat aagcttgctg caagtttgca agataccaga tttatttcat
    18241 cactctccgt taccgcaaga atcatatcag catcttcagc gccggcacgc tttaatactg
    18301 aaggatagga ggcatttcca acaacaaccc tgagatccaa acgatcttgc aacaaagcaa
    18361 gctgtcccga aacaatatca actacagtaa tatcattcga ttcactaacc aagctttctg
    18421 ctactgtaga accaactcgc cccgcaccta aaataagaat cttcaaattg tattactcaa
    18481 tcttttaaca agaaaagaat agcttcttga atggtaaaca tactcactta tttgagcagt
    18541 aaatattgct ccctaaccca atcacgccac tgttcaaaca attgcaaatc taaagcggca
    18601 ataactgagc gctgccacgg tgtctgtaac caataatcat catgctcaaa accgcacata
    18661 tgacctcctg cttcctccag aataaggctt cctgccgcat aatcccaagg cttttgtcca
    18721 ccatgcaaat agagatcaaa ataaccagca gcggtataac accattccag ggcgcaagca
    18781 ccgtaattcc gctgcgaagc ataaggaggg ttagctgcta cttttgcagc taattttcga
    18841 gataaccgtt ttaaatcaac atttgccaca gcgctctgta atgaagcagt cgattttctt
    18901 atcggcaagg caactccatt gaggaaagca cctttccctc gctcagcata gaatagttct
    18961 tttgtggctg gattataaat aaccccaagt aagcctctcc cgttttgcat caaagcaaca
    19021 gaaattgcaa aataaggaat tccgttataa aaattagacg tgccatcaat cggatcaatg
    19081 ctccacaatc ctgcagctcc gtcttcccag cattggagtt gctgttgttg cgacatttct
    19141 tcgcccatca caactacagg atatagcttt gttaattctg tcactaagct cgcttgcgca
    19201 gcaagatccg cttctgtata gaaacttcca tctcctttat gttgacgact tacctgcata
    19261 aagcgtggca taacatcctt ttctgctacg cgcttgacta tggtaataac ttgctcaagc
    19321 atttccatca gtacgccact ttatcgagca accaatgctc ggtatttgat ccatcggccc
    19381 tttaccagtt tttgccactt gtaccatcgc ttcaaataaa tcacgatgcg cgtctaaagg
    19441 agcagcttcc tttcgtgatg catcaaaacg tcctcgatat tgaagctcta atttatcatt
    19501 aaatccaaaa aagtctggtg tacaaacagc tccatagcgt tttgcaattt cttgagattc
    19561 atcccagaca taaggaaaag gataattata ttccttagcc actttggcca tgttttcaaa
    19621 tgagtcttct ggataatcca atggatcatt agacatgatt gcgatggaat taatgccata
    19681 cgcctcaagc tcttttatat cgcgaataat acgatctctg attgctttca cataaggaca
    19741 atgattacat ataaacatca ccaatagccc actccttccc ctggatgaag acaagctata
    19801 tcgctttcca tcgaccccca gtaaatcaaa atcaactgct ttccatccaa actcacaaac
    19861 tggtgtttcc aaactagcca taccgacttc tccaaactat tgttcttttt aatatctata
    19921 atattatcat ggatcatgat caacccgttt tcacgcacat caatccactc actatgcccc
    19981 cacgtttcta ttgtaatcac ccactaacta ttaacgaaac tatcttgctg ccaagtggag
    20041 tttcacatca tgcagctcat gttttaaggc tcaaaaagaa caatcaagtt attttattta
    20101 acggtcaggg gggtgagttc acaggtcaaa ttgagcaaat tgataaaaat ggtgtgacag
    20161 tacaaattca acagttccac gatattaatc gtgaatctcc tgtgatgact gaattagttc
    20221 aagctatttg tacaaatgaa aaaatggatt ggatcattca aaaagcagtc gaagttggtg
    20281 ttaatcgcat ccaaccgatt atgacaaatc gttgcgtcgt tcatcttcat ggagaaaaag
    20341 ttaaaaaacg agccgaacac tggcaacaaa tcatccattc ggcttgtgag caatgcggaa
    20401 aaaataaaat acctgagctt ttgccattag tttcatatac tgactggctt agccaattta
    20461 aaaacaatca agctgatcgt tcaattacat catcaaccaa attaatgctc acaccaacag
    20521 ctcaaataaa attgtcagag ctccaaccca acaacaaaga tttttttatt ttactggtag
    20581 gacctgaggg tggtttaacg ctctcagaag aaaagctagc aattactaca gggtttatac
    20641 tgattcgcct cggtcaaaga acattgcgaa ctgagagcgc tggtttagca gcattagcaa
    20701 gtatccaggc tctttggggc gattattgat caaattaggg taatttttat aacaaaagaa
    20761 tcattcttaa cgctgccagc aggtagccgc atctagagta gcgccagctc ctaccccttt
    20821 tacaccagta ttcgtcagtg tcaatgttga acatgcatca ccggtcattg atccagtcgg
    20881 aactgcttgt aaagtatagg cattctgagt aagtgaacct gctgcaaatt gaatcgtata
    20941 ttttgcagtc ccagttttgg gagattgtgt tgttgataaa gtaaaaccag tcccatcact
    21001 tttctgatca taccgattca cagctgtata gttccgttcc aataattgtg acatctcgag
    21061 caaaatactt ttagcttcag cacgatttgt tcggcgcaca tgatcttgat aggatggaag
    21121 tgcaaccgct gcaataatac cgagtaccgc tactgcgatc attagttcta tcaaggtaaa
    21181 acctgaacac aaaaaatgtc tctttgcaaa agagttaggg caatgacgat aaatggaatt
    21241 cattatttct cccaaataag caatttaaac tcagatcaag ttcttattaa aaaatttgtg
    21301 atgcaataat ctactgaatt tgccgccaag aacgtcttgg agtcagtgga tttccactaa
    21361 ccgtaattgt ttcaaccgaa ccggtcgttg tccctgcgat caaatggagc aagttacctg
    21421 cactaatcgc aaccgactca cttcgaaccc catcagatcc tacaccagca ataatttgat
    21481 cgctactgtt cagtagaccg tttttatctg tatcaaaatg aggtgctgtt aacatccctc
    21541 ccgtttcacc attcaataac atcacccaat tgctaccacc ggatgcgcaa ggatcacttg
    21601 aaggaattag agttgtaaat aagattcttc caaaattaag gagtggcatg acaatagagc
    21661 gttctccttg attacttgaa ctgggaggtt tcaataaatc catataccac cctcgcttac
    21721 tcgcccagtt aaccgcattt gtagagacag ttcgtctgaa tggcgaagat gttgtttgag
    21781 tttcaatcgt ctgctgctgc aatgtactac gattcgtttc acttattctc gtaccattat
    21841 cccaaatacc atacaaagtc tgaacttgag tattggtatt gtcgccggat gcaataaatt
    21901 gaccggttcc aaaataaata aaaaaaccac cattcggatg cactccaatt tctagtggag
    21961 atgtaattgg ttgtacctga ttactcgagt tccttgctgt aaataacgga tatttggttc
    22021 caccactacc atatgccaca tcccattgcg atgtattcgt atgcgaaata tcaaatttcc
    22081 acacattacc ttgcaaatct cccgcataaa cataatcgat cattttgtcg ccattggtat
    22141 ccacaagcgc gggtgcagat aatccattac tagttgtact gttagttgcg attttcttaa
    22201 tcagtgcccc agtttgtaga ttaactatat acaaaaaggc tttgtcactc gtactattat
    22261 agccattact aataatcgca acccaatcac cattattcaa aatagcaatc tttgcctgac
    22321 catgtacata accgagatcg gcatctgtaa actcccagag aacattagaa gctgaaaaat
    22381 tagttggatc agtaatatca agcgcaaaaa ttcccttacc acccccacca gtcgttccta
    22441 ataaaatagt tctccacgag ggtgtcgctc ctatatacgc atctccggca tacgcattgc
    22501 catccactat atagcgatgt gtatagcttg gtgatgtcaa agaacttaat tttgaataaa
    22561 ctgaacttgg tacataactg aaaagctcaa cgcctgtatc agcattaaaa gcatgcagca
    22621 ttccatcatt tgctcctgta taaataacag gagttctact ttcatttgct tgaacgtaat
    22681 tataataagt ggtttgacct ggcgttcccg aaacgagacc atcatagcta aaatttaatg
    22741 acctaacaaa aaataaatca gaattgatga tatcaccaag tattcctgca ccgcgattac
    22801 gaaaacttcc cccttgtgct aattctgtcg acttatctcc tctcaaccaa gcaactcgat
    22861 tagcaccttg cccatcatta accccaccga tatttgtatt taaggcattc tgctgagaac
    22921 tggttaataa agaaaaatta ctcgttgtga atgcaattcc attggttcca ttatgcgtaa
    22981 aaatttttcg acttgagtgg ttcggtataa ccgcggacgc ttcccattgc acagttccta
    23041 tggaaccatc ggaattcaaa ttataggata gtaactgacc actccaatca ctgctattaa
    23101 acttagcttg atagatacga gtacccgagt taagccggct tgtattagtt gttacggctg
    23161 ctgcagagct cgttctagct gaaatattat ttagaacctg acttaaacca gttgcgaaag
    23221 cagtaggatt ttgagcacta aaaaactcac cacgactatt taccgccgca tgccataaat
    23281 cgtctatttt ggcaccatta ctagttgttg gatctggcca acttccagcg cctgatggca
    23341 gctcagttaa agttccacta acaccgagtc caacagtaaa attcaccatg tgttgccaga
    23401 aagcaggatc cttaccgttt gtaggaacct tattggctaa atcagtcctc aggtcagtct
    23461 tccaataata cattgcaaca tcagccaatg tgtcactgaa gctatcagaa taaggtaacg
    23521 ttggagtata tttataagtt gcaggagcag cattgggtgt atcgttggtg attgagctac
    23581 cctctgtatt gtcagcatta ccaactcctg aaaacccatc attccagtaa ccatcagtca
    23641 ttaaaatatg ataattttga cgacacacgt attgtgttcc accgcttgaa ccaggcgttt
    23701 ctccccaagg acctttatca tcggttcttt gaaaatatct accaacatca tcgatagccc
    23761 atcgaagtgg ggtgccatac gtataaattg gatgattata aagatcgcta aaaaaagttg
    23821 acttattact agaagtaaat ggtctaacgc ccctaacaat ggtataagcc attaccccat
    23881 ctatactggt attaccttta tttattgcgc caaaacccac ccgcatatta ctcccttgtg
    23941 cagcaaatgc cttgccaatt cctgcacgtg aaagcagtat ccgagagcgg taataagtat
    24001 accaattagc aaaattctga atctcttctg cataactaca agtgggtgca cttgcacaat
    24061 cagtgcgatc aacactacct gtataagtcg caatcgaaga tttaatttct actttcgtat
    24121 aactagtcgt cgacaatatt gccccgctct gataaagata atataccgca ggataaaaag
    24181 ttttagatga ggttgaaaga ctagcatcac tatttaacca actggctgtt tccgtattat
    24241 tggttgtcaa atccctacaa ccaacaccgg tattagtggg attatggggt gcacaggtaa
    24301 ttgatgcatt actcatcagt gagccatctg cattactcca gggttggtac cttattgctg
    24361 gatcataata aatcttatta acgtaactgg aacgcaatgc aactgcataa gggttatttg
    24421 aatcaaaatc tactgaagca ttactataaa cattcccact ataaatactc tctgcacgtg
    24481 gaaacatata aacaacccca ccgtatattt tctcctctgg catgatttca aactgcatgg
    24541 atccagaatc gtcaagcgta aacataatat ttgaatcaac cgctgtagtc agaaataaag
    24601 gtacatctga taacgccagc gttgcatgag ctcgatatgg aattagcaag gagattagca
    24661 atacgataat aaatcccgta aaaacttgat tcttcaatta agccactcct ttgaattatc
    24721 gacgataaat actttgcagt acgactactg ctgaatctgt gccaccgact ccccttgcaa
    24781 tcacgcgata catattagac atatttgtta ctggcgattg ttgtgcgcta cctttattcc
    24841 cctgaaaggg gacactgaca agctgttcaa taatatagcg tggctgactc gctgctcccg
    24901 taatcgatgt gcttattgct tttcctgaag tcgtccaacc tgtccatgtc attgggtcgg
    24961 gcgcaggaga cgatgcatga tgataaagcc catcagaacc attaaaagta ggtagcgtta
    25021 tttgttccaa gaatcgctcg ccttccctta aagcagcctc agcagcttgg aacgcaagat
    25081 tttcatcacg aagatttcca gccattcttt cctctaaaac ggtattttgc atcgccgtta
    25141 caccaagaat agtcatcatg accagaaaaa ccaaacccgt gataagcact accccttttt
    25201 gattagtcat tgatatttct tgatttctca tttgtatgat gttcaacaat ctgttttaag
    25261 aagtgcgatt gcgcaatgta actacggtag taaacacttg gcgtaatctc cgatcattgg
    25321 gtgttgttat ggttccatta aacgtataag tttgcgcatc agtagctaca ttattttcca
    25381 atgactgcat caaaagactt attcgaacac taacaacttc agcccaattc gtaactgcat
    25441 ctgctgtacg ataaacatct gctgttgagt taccatccaa atcttcccca tactcgattt
    25501 gcatatcctc aacaccttca accaattctt cagcattacc tgcacctacc ttacgataaa
    25561 gcgaaggaaa aggcgttgga ttagaattca ttcggataaa ataagagcgt gtagaaattt
    25621 tgatcaactc cccacccgta aattcttttc ccagcgcttt agtaaggtta ccaggcgtgt
    25681 ttgcggttgg atttccagtg ttatgaacaa cattatcttg cccggcagaa gtatttacat
    25741 tggtaacttg gaaaacagca gctgcaaggc aatccgtaac aagtacaata tcgtcttctt
    25801 gaaggccgct gcctaccgta actttcaaat cagcggatcc aggtgggtta ccgcctgggt
    25861 gttgaatcac tttaacatcc gtaccatcta ccccccgaat cacaacaata tctctcccac
    25921 ccagaggact gggaatagtc cctgatggtg atggtaatgc aggagtccat gccgaagaac
    25981 tacttgcttc aaatccttct atgggttgcc ctagcttcca aagaaaatct gatggattgt
    26041 ttaaagtatt aacaataggt actccatccc ctacacaacc taagtatccc gccatgcgga
    26101 tatcacgcga tagaagctgg aatgcatagc gagcattttc ttgtagccga gacaatgcgt
    26161 cattgactcg atatgtttgg tggctagacg atagtattgt aataacacca cccagcaata
    26221 acagcccgat cgtcattgca accattaact ctattagtgt aaatccatac tgctggttga
    26281 ttactttttt catttgcacc tcagagccgg gtagccatca tgaattgctg agcagccatg
    26341 gagcctcgac tgtcatccca ctgaatagtg atcgtcacat tattattgtt atccacattt
    26401 actgatccca accctgtagg taatcttaac gctaattcag tcatccaatt attgagatcc
    26461 ttctggtatg ttgcatcgcc agtaggttta gctgctccca aggcaatgtt atagttacca
    26521 ttactcgcct cagttcgatt agcacgcatg ctatcaagaa tgctataggt catcattgtc
    26581 gctgttgaac gaaagctcgc actctggtta tgagtaagtc ctgcggattg gagtccagca
    26641 aggccaagca aaccaataga aagaacaatg actgatacca acacttcgat catcgttaca
    26701 cccttttgtg tagctatttg attttttata ttggtactta gcatgtcgtt accgatttat
    26761 ctatttgcgg acggcctgta ccgtttatta caacatcgcg ccttttcgca ctagcacaga
    26821 acctaaatgt atcgttctga aaattacctg ataacgcacg gccattcggc atataagaaa
    26881 gccaattctg aaaatttcca ccactcatta gtgtacttcc tttgagcgca ggaaaaactc
    26941 gaagtacctg atctgctcca tcgaccgttc ctgaaacacc cgtatcagta aatacaatcc
    27001 agccatcgtg ccaattgctc gctgtcgtgc agctggctcc attattgctt ttacagatcg
    27061 tcacccttga acctcgctta attgcttcag atcgggtaaa gtgaagcgtt gttacaagtt
    27121 cattggtctg ggtcgtcaag cgtgattgaa ccatcatgcc ctgatagctt ggcacagcaa
    27181 cagaagccag gatagcgagg atactgaggg ttatcatcag ttccaacgtt gtaaaaccac
    27241 catttacaat taaatgcatt attattcctg ttcattcttc atccacacaa agtaacggtt
    27301 attaaaaaat caactttaat tgattaaaaa atgacatcta cttaataaaa cggaaaaatc
    27361 atctaataaa atgtcacaat tcttgcttat tggtgctgga attattggcc ttacatcagc
    27421 agtacaccta cttaaggagg gattttctgt tacagttttg gaaaaaggag aagtaggcca
    27481 agaatcatct tgggctggtg gtggcatcct atcgccccta gccccgtggg attattctga
    27541 gcctgttacg caacttgtag gctacagcac aagcatatat agcaagtggg tggacgagct
    27601 ctcgttatct acagaaatag accctgaatt caacgaatgc ggcatgttag tgctacctcc
    27661 ctatgattca gtaaaagcac atcaatggtg catttcacac aatatcaagg aagaaataaa
    27721 ccataaatct ttctggagtc caattcacca atctgctcta gaagaagcga tctttcttcc
    27781 tcgtgttgct caaattcgca acccacgctt attagctgca cttcaatgtg aagtgattaa
    27841 gcgaggaggc gtgattaaag aattttgtga agtaagtaga ctaaatctaa aaaataagtc
    27901 cgttgtatct attagcacaa ctagtggtaa ttattctgca gatggctaca tcattactgc
    27961 aggagcatgg agtagaaaaa tattaggaaa atacgcatta aatctcaata tcatcccgat
    28021 acaagggcaa atgctattat acaagtttga tgccccacct atcccgtcta ttctcctcca
    28081 aaaagaacta tatctgattc caagacttga tgggcactta ttgattggaa gtacaatgga
    28141 aaatgttggg tttaataagc aaataaccca ggctgcctat gataagttat ataaaaaagc
    28201 atcacgactt cttccagaaa tagaaactaa gcacctaatc aaacattggg ctggcttaag
    28261 gccagcctcg ccctctaata ttccaaccat aggacgtcat ccagatttac ataatctctt
    28321 catcaatagt ggccactttc gctatggcgt aactatggcc ccaggaagtg cagaaatcct
    28381 tttaaacgaa ataatgggaa agccgtagcc ttttgatatt tctttttatc aaaagggatg
    28441 gaatcagcta ggaaaaatcg aattctccta aaacttaaaa ttgcttttct aaagcaatag
    28501 cttctgcctt aggtaaagga aaaacaactg attcaacaac
//
LOCUS       DAOQLJ010000011        27599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_567_length_27599_cov_6.064842, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000011 DAOQLJ010000000
VERSION     DAOQLJ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 27599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27599
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_567_length_27599_cov_6.064842"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PK522_01720"
     CDS             complement(<1..735)
                     /locus_tag="PK522_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40484.1"
                     /translation="MNKLEKYKQKYFRGFLFFLSLWFLINPLKALELSPYAEGPLLEI
                     ITQMPEGTWKKVNANSFSEVWTPPELRPLNLLSNPPPSKLISAWSSFAWDSNRGDLIL
                     YGGGHANYSGNDVYRWRSRDLKWERASLPSQIAVIAGTSASTAIDGVDAAPASAHTYD
                     NSIFLPIIDRYLVLGGALYNTGGPYIRQSETDPTVTRITGPYLFDPEKASANQVGGTT
                     GSHVQRVYAFPEITGGEMWQNRDIHKH"
     gene            complement(931..1764)
                     /locus_tag="PK522_01725"
     CDS             complement(931..1764)
                     /locus_tag="PK522_01725"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HNC40485.1"
                     /translation="MIKQRRTVFFLSDRTGITAETLGHSLLTQFDGIEWVKVSIPFLD
                     EIGKIKVAIDQINQIAVQEGVRPLVFSTLLNPEILEQVRQANCRVYDFFAMFMGSIEQ
                     ELNQPFARIAGRSHGLQHHLTYFKRINAINYVLAHDDGVNPKNFAKADIILIGVSRTG
                     KTPTCLYLAMQYGIAAANYPLTQEDMGSMRLPKVLESVQDKLFGLTLSAAQLHLIRQE
                     RRPNSEYASLAQCQHEIQWQQSLFKLMNIPYLDTTAISIEEISAVILDRCGLKRQLYG
                     L"
     gene            complement(1767..4145)
                     /gene="ppsA"
                     /locus_tag="PK522_01730"
     CDS             complement(1767..4145)
                     /gene="ppsA"
                     /locus_tag="PK522_01730"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNC40486.1"
                     /translation="MKAYIKWFDELSMRDIALVGGKNASLGEMYSHLTKLGVNVPAGF
                     ATTTEAYRDFLEANDLSQRIKHELDQLDVDDVAMLAITGKAIRTWLIEAVLPDQLLEA
                     ISIAYEKLEKSAGGEVVSFAVRSSATAEDLADASFAGQQETLLNVSGLDQIIQAIKIV
                     FASLYNDRAIAYRVHQNFPHDKVYLSVGIQKMVRSDLGASGVMFTLDTESGFQDAIFV
                     TSAYGLGETIVQGIVNPDEFYVYKQGLRSGKSAILSRRLGTKLIEMVYENSFKQDTFT
                     VTRDVSVERRQRFSLSDQQIEQLGQQALLIEQHYGRPMDIEWALDGLDGKLYIVQARP
                     ETVKSRAGTSLERYHLTQKGMVLSEGRAIGQKIGQGTAKVIFSIDQMHEIQSGDVLVT
                     DMTDPDWEPIMKRAAAIVTNRGGRTCHAAIIAREMGIPAVVGCGEATSQIKPGSSVTI
                     SCAEGDTGYVYEGLLAFEHSVADMGQLPQLPIKMMMNVGNPSHAFAFSQIPNQGIGLA
                     RLEFIISNMIGIHPKAVLEFDQLPSDLQALILGRSAGYANPVQFYVEKLVEGIATLAA
                     AFYPNPVIVRTSDFKSNEYANLIGGERYEPKEENPMIGFRGASRYISPQFQACFELEC
                     QALRKVRDEMGLTNVEIMIPFCRTLEEAQQVTALLASQGLSRGRNGLRVIMMCEIPSN
                     ALLAEEFLQFFDGFSIGSNDMTQLCLGLDRDSSLVAHAFDERNPAVKKILKMAIDACR
                     TQGKYIGICGQGPSDYPDFAAWLNEVGISSLSLNPDSIVETWLYLASLTKQN"
     gene            4429..4605
                     /gene="ssrS"
                     /locus_tag="PK522_01735"
     ncRNA           4429..4605
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK522_01735"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            4775..5371
                     /locus_tag="PK522_01740"
     CDS             4775..5371
                     /locus_tag="PK522_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNC40487.1"
                     /translation="MNFIKINLLIPFTLSLSCALWFSGCVPLVATGVGVGAGTGALMS
                     EDRRSTGIFIEDEAIELKTSRRITQQLGGNVHINVTSFNRNVLLTGEAPNEATKSEIE
                     KLALSIDNVRKVFNEITIAENSTLSSRTNDSIITSKVKGRFVSERKFRVNYVKVVTEN
                     SVVYLLGLVTREEGEDASQIASTTSGVTRVVKVFEYLN"
     gene            5404..6771
                     /locus_tag="PK522_01745"
     CDS             5404..6771
                     /locus_tag="PK522_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNC40488.1"
                     /translation="MLPSELVAQIQKIIPSERFYTDPVDCYAYGYDNSRKIFPPEAVV
                     FPITTDEVVAIVSLCNRYKIPITPRGRGTGTAGGSIPDQGGIVLSLERMVKILNIDPG
                     NRVVVSEPGVLNQEIQDTVKPYGFFWPPDPSSANFSSIGGNLATSAGGPHAVKYGTTR
                     DHVLGIKAVTATGDIVKTGCYTTKGVVGYDLTRLLIGSEGTLAVITEATLKLTPLPKC
                     KSGLVAYFQDATSCAKAIVAIMSLPQGPSALEFLDKNSLNLIRNRFPELIPIEANTLL
                     IIEVDGSENELSEAMATIKSSCDIDGILSIEHSNNPHDLWKARKALSPLLRDIAPKKI
                     NEDIVVPVNSLPQFLDDLDKICTKFQIANVNFGHAGNGNIHVNLLIDPNNSDENLRAE
                     ACLDEIFDLVIALKGTLSGEHGIGREKRAFIHKEIDDPTLTIMKSIKTLFDPNNILNP
                     GKIFP"
     gene            complement(6886..7782)
                     /gene="cyoE"
                     /locus_tag="PK522_01750"
     CDS             complement(6886..7782)
                     /gene="cyoE"
                     /locus_tag="PK522_01750"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNC40489.1"
                     /translation="MSVNTSTSQSVLDRLQQFYRLTKPRVVSLIVFTAVIGMFLAVPG
                     AVPLDTLVLGTIGIAFVAGAAAALNCLVEQKFDAVMARTRGRPLPQGKVSVPETLFFL
                     GLIGGLGLFILHQWINPLTMWLTLGTFVGYAIIYTVILKPLTPQNIVIGGASGAMPPV
                     LGWAAVTGEVTADALLLFLIIFAWTPPHFWALALYRKTEYAQIGMPMLPVTHGDKFTR
                     LHVLLYTLILCVVTILPYVSQMSGLIYLFSAIVLDAIFFYYAIKIYLNYSDQLARAAF
                     KYSILYLALLFTALLVDHYFYI"
     gene            complement(7816..8400)
                     /locus_tag="PK522_01755"
     CDS             complement(7816..8400)
                     /locus_tag="PK522_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40490.1"
                     /translation="MSNEVARSNRTKFIALILLMLSPVMASVILHNIKFRPDSTVNYG
                     ELLEVKAIHGQAHEIENNTIFRERQLHGKWSFAMIDSGNCEEYCQKKLYQMRQVRLAQ
                     NTEKDRINRIWFIDDQIVPNQEIKKEFEGTVLLTVNDNKFLDQFPAVSSRKDHIYVID
                     PMGNLMMRYPRDANPSKMVNDIKLLLKLSHLEHK"
     gene            complement(8372..9094)
                     /locus_tag="PK522_01760"
     CDS             complement(8372..9094)
                     /locus_tag="PK522_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HNC40491.1"
                     /translation="MIIKDYQFKPKWWILVLSVILIIIFTRLGLWQLSRADEKDSKQQ
                     LLDQLAHEAVLTLPSSRVELSDLLFRNVEVRGQFIASHTIFLDNKVYRGAVGYHVLTP
                     MRIENSNMHVLINRGWVALGKNRSTLPEIFTPTDVITLSGIALSPTIRTMKISDEIST
                     GKVWLAFNFEEYQAASKLELQPILLMQLDNSINDGLIRELERQDLGASKNMSYAFQWF
                     FLAITVFILLLALNVKRSSPQQ"
     gene            complement(9212..10060)
                     /locus_tag="PK522_01765"
     CDS             complement(9212..10060)
                     /locus_tag="PK522_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNC40492.1"
                     /translation="MSHGTGHYYVPSPSKWPIVGSSALLFLGFGAAFTMNKIESGYWM
                     LAIGFAILTYLLFGWFGEVARESESGKFGKQEDKSFRWGMSWFIFSEVMFFAAFFGAL
                     YYMRQLSVPWLSDMEHQLIWPGYTGGWPTAGPGITEAFTPMGAWGLPAINTMLLLTSG
                     VTITYAHWALKKNDRSGLKKWMLATVALGIAFLCCQIYEYGHAYADLNLKLTTGAYGS
                     TFFMLTGFHGFHVTVGTIMLIVIYFRCAAGHFKPEHHFGFEGVAWYWHFVDVVWLGLF
                     IFVYLL"
     gene            complement(10090..10287)
                     /locus_tag="PK522_01770"
     CDS             complement(10090..10287)
                     /locus_tag="PK522_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HNC40493.1"
                     /translation="MQKSEKASLWQVVKAVFWSFVGIRKKSDLEKDAEMIQPSQVVIG
                     GLIGGLFFVVSILLVVKWVTS"
     gene            complement(10320..10850)
                     /locus_tag="PK522_01775"
     CDS             complement(10320..10850)
                     /locus_tag="PK522_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HNC40494.1"
                     /translation="MMYNVAKINIAMLRKLLVFSVIMFAFGYALVPFYEKFCEVAGIN
                     NLLKPDVADKDIKVDTTRLVMIQLDSNVRGLPWQFKALQSNIRIHPGESIEVMYEISN
                     NSDQETSGQAIPSYSPRVLEKYLKKFECFCFTKQVLKPGETRQMPVKFVIDPQIPADI
                     TTMTISYTFFSLGAVN"
     gene            complement(10980..12560)
                     /gene="ctaD"
                     /locus_tag="PK522_01780"
     CDS             complement(10980..12560)
                     /gene="ctaD"
                     /locus_tag="PK522_01780"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HNC40495.1"
                     /translation="MAVVQDVHGHGHDDHHPTGIMRWVCTTNHKDIGTMYLWFSFAMF
                     LVGGFLAMGIRAELFMPGIQILEPELFNSFTTLHGLIMVFGAIMPAFVGLANWQVPMM
                     IGAPDMAFARMNNWSFWILPVAATLLVSSFFVPGGAAAGGWTFYPPLSTQGGMGVDMM
                     IFAVHLLGASSIMGAINIITTILNMRAPGMTLMKMPLFVWTWLITAYLLVLVMPVLAG
                     TVTMLLTDRNFGTSFFNAAGGGDPVLFQHIFWFFGHPEVYIMILPAFGVVSEIIPTFS
                     RKPLFGYSSMVYATASIAILSCVVWAHHMFTVGMPSVGQLFFMYATMLIAVPTGVKVF
                     NWTATMWRGSMTFEVPMLFSIGFIFLFVIGGFSGVICAIVPIDIQVQETYYVIAHFHY
                     VLVSGALFAIFGAVYYWLPKWCGRMYDEKLGKWHFWLSMFFFNLTFFVQHFLGLAGMP
                     RRVPDYALQFADFNMVSSIGGFGFGLTQLFFLYIVIKCIRSGEKAADKPWEGGTTLEW
                     TLPSPAPYHSFETPPTIK"
     gene            complement(12613..13431)
                     /gene="coxB"
                     /locus_tag="PK522_01785"
     CDS             complement(12613..13431)
                     /gene="coxB"
                     /locus_tag="PK522_01785"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNC40496.1"
                     /translation="MSSKSVATRVGAALLTFYSSLAMSSKYNFPEPQSAIAQDVYDQH
                     IFAMWICLAIFIAVFGVMFYSILKHRKSLGYKAANFHHSTLVEIIWTAIPCAILVVMA
                     LPATKTVLAMKDTSEPDITIKVTGYQWMWGYDYLQGEGEGISFYSKLSTPRAQIENKE
                     PKGENYLLEVDNNLVVPVGKKVRVIVTANDVIHAWWVPAFSVKQDAIPGFVRDAWFKA
                     DRTGIFRGQCTELCGKDHGFMPIVVEVVEPEKYAQWVADKQKASASAMTTAALN"
     gene            complement(13968..16355)
                     /locus_tag="PK522_01790"
     CDS             complement(13968..16355)
                     /locus_tag="PK522_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNC40497.1"
                     /translation="MNRPVTLEPVSGQNLDINYQLDFSKRLQAVAARINAANNIDEIM
                     LEVSKDICALFNADRLTIYTISEDKSYIVSRVKTGLDSFQDLKLPITEQSIAGFAGLN
                     KKIVNIADVYDRGELMKLSSQLHFLKEVDKRTGYRTKQMLAAPILNVGDNELIGIVQV
                     INNKEDKPFSQIVVAGISEICQILAVAFKQRQKQQYLARSKYDHLISDSIISAAELEM
                     AARNAREKGCDLEDILIDEFQIKPSQIGEALSLYFKVPYEPYRADRIKPMEILKNLKR
                     DFVESNQWLPIDDAKEGLIVLALDPERIKVLRMVNQVFPKHKIVYTVCTHPEFKATLD
                     LFYGGVGSTNLVDTGNINDMLFDLKEGEEEVVVETDETSAASDNELVKLVNKIIMDAH
                     RMGVSDIHIEPYPGKEKTKIRFRKDGSLMPYIEIPASYRNPLVTRIKIMCDLDISEKR
                     KPQDGKIKFKKFAPLDIELRVATIPSAGGLEDVVMRILAAGEPIPLEKMGFTPRNLDE
                     LKTIISKPYGLFFVCGPTGSGKTTTLHSILKYLNTSETKIWTAEDPVEITQKGLRQVQ
                     MNVKAGLTFPVVMKSFLRADPDIIMVGEMRDKETTSIGIEASLTGHLVFATLHTNSAP
                     ESIIRLLDMGMDPFNFSDALLGILAQRLAKRLCSKCKKPHTAGSDEIQSLLAEYCEEL
                     KNIERFRTNPDAAYKRIEGDWIKQYGNEQGQITLYQSVGCDSCNGTGYSGRVGLHELL
                     TASDALKKNIQEHARVADMLVTALSEGMRTLKQDGIEKVLQGITDIHQIRAVCIK"
     gene            complement(16375..17139)
                     /locus_tag="PK522_01795"
     CDS             complement(16375..17139)
                     /locus_tag="PK522_01795"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HNC40498.1"
                     /translation="MKLRVLGCSGGIGKGSSTTSMLLDDDILIDAGTGVSNLTLDELV
                     KIKHIFVTHSHLDHIALIPFLIDTVGCLREFPLIVHAIPPTLEAIQKHLFNWEIWPDF
                     TKIPNGENPYLTYKSLAIGNEVDLEGRKITPLPANHVVPAVGFRLDSGMASLVFTGDT
                     TTNDALWVEVNKIQNLKYLIIEVAFCNAKKDIAMRSKHLCPSMLAEELEKLEQEVEIF
                     ITHLRQGEADLTMQEIERCAGRFNSRRLMSNQIFEF"
     gene            complement(17224..17649)
                     /locus_tag="PK522_01800"
     CDS             complement(17224..17649)
                     /locus_tag="PK522_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNC40499.1"
                     /translation="MLKMLLPVDGSEACSKAISQFISILGWYKELPTIHLLNVQYPLQ
                     GDISMFISQDNIKQYHQEEGLKDLMQVRKLLDTAKVAYQYHVAVGEPAETIIQFAQAQ
                     QCDQIVIGPRGLGVVTGMLLGSIASKLIHLSDIPVLLVK"
     gene            complement(17696..18418)
                     /locus_tag="PK522_01805"
     CDS             complement(17696..18418)
                     /locus_tag="PK522_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNC40500.1"
                     /translation="MTIYEPEFWVAALQIIAIDIVLGGDNAVIIALACRKLPEKQRNQ
                     GIFWGVFGAIFIRVLLVFFALNLLTLPFLKIVGALLLIWIGIKLLMPESSDNEHQVDA
                     GTTLLGAIKTIIIADTVMSLDNVIGIAGAAKGDIGLVVFGLLISVPIIVWGSKFVMKL
                     MTRFPVTIVIGAGLLGWIAGDMSTTDTVTKVWIEDHAAYLRWVAPIVTTLIVIITGKW
                     LAARAVRKAMVLTNLGDNNKSI"
     gene            complement(18437..19594)
                     /locus_tag="PK522_01810"
     CDS             complement(18437..19594)
                     /locus_tag="PK522_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNC40501.1"
                     /translation="MSDHDTTTTNPLPKIGLVLTGGGARAAYQVGVLLAIAEMVPAHA
                     RSPFSIICGTSAGAINAAGLAMSATHFSTGVRQLQKVWCNLHVDMVHRADLLGLIRNT
                     LHLLGSLFSSGLARRPVSLLDNAPLRSMLACRLPFRGIQRSIHAGALHALGITVWGYT
                     SGQSVTFFQGNKSILPWKRSQRVGVASYIGIQHLLASSSIPFIFPAVRVNREYFGDGS
                     MRQFAPLSPALHLGADRVLVIGVNPKDIVTSERIKVTSYPPLAQIAGHAMNSIFADSL
                     NVDLERLNRINETLALIPPNARKESSLTLRPIQSMVIAPSQRINEIALQHAHQLPHTM
                     RYLYRAVGAMGSSGSGLLSYVLFEASFCSALIDLGYRDAMQLRTEILKFIQ"
     gene            complement(19587..19844)
                     /locus_tag="PK522_01815"
     CDS             complement(19587..19844)
                     /locus_tag="PK522_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40502.1"
                     /translation="MIHTQRQEQELSMLRSEIEMLMSERQSLLRTVGAAAVFIANLDD
                     KILLGDICKSAKVLSASLNDLPEETLSDALEKVNSKFISCE"
     gene            20003..20956
                     /gene="pip"
                     /locus_tag="PK522_01820"
     CDS             20003..20956
                     /gene="pip"
                     /locus_tag="PK522_01820"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HNC40503.1"
                     /translation="MKLFELYPEIEPYNQGMLPLDTMHTMYWEQSGNPSGIPVLFLHG
                     GPGAGSTSHHRRFFDPASYQIVIYDQRGAGKSIPLGEIRNNTTPHLIEDIEALRKHLG
                     IDRWLIFGGSWGSTLALAYGESYPDRCLGFILRGIFLCRKSEIDWFLYGLRTLFPEPW
                     EEFVSELAPSEQKDILATYYTRLMDLDPAIHMPAARAWGRYEGRCSTLLPSSETVSYF
                     SSDGVALGLARMEAHYFKHNIFLPENSLLDNISKLQHHPAIIVQGRYDAVCPIVSAND
                     LHHAWPSAEYLIIDDAGHSAWELGIRSALVEATEKFKSIDR"
     gene            complement(20969..21637)
                     /gene="bioD"
                     /locus_tag="PK522_01825"
     CDS             complement(20969..21637)
                     /gene="bioD"
                     /locus_tag="PK522_01825"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNC40504.1"
                     /translation="MSIGFFVTGTDTNVGKTLVSCSLIHAFAKTRRSVVGMKPIAAGC
                     ENGRWLDVDQLSAASTRQFDRAMINSYHLLAPVAPQIAANYEAVDIDIAVIQQAFHIV
                     QKAVDIVIVEGVGGFVVPLTSQTDTSDLAVALNLPVVMVVGLRLGCLNHALLTAEAIR
                     ARRLPLVGWVANMIDPQMDDITENIEVLRRRLACPLIGILPYNPHIDASVSANLLDIN
                     PLLM"
     gene            complement(21645..22529)
                     /gene="bioC"
                     /locus_tag="PK522_01830"
     CDS             complement(21645..22529)
                     /gene="bioC"
                     /locus_tag="PK522_01830"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HNC40505.1"
                     /translation="MIYEHSLDKRLLRRSFEKAAETYDHAAVLQREICDRMLSRLDFI
                     KYLPDFILDMGSGTGYGTQKLRERYDRSTIIALDIALNMHLQAYRPISGWRKWISLVK
                     RHSIDYVTGDIEQLPLQKESVNMIWSNLALQWCNDLKQSMTECHRVLKPGGLIMFSTF
                     GPDTLSELRKTFKAVDSYQHINRFIDMHDIGDILVHAGFAEPVMDMEYITLTYQDVAS
                     IMRDLKAIGAHNVMIGRKKGLMGKRAWQQIIDRYELLRQSGKLPATYEVVYGHAWKPE
                     SKLALLKATTKQKLGISE"
     gene            complement(22519..23277)
                     /gene="bioH"
                     /locus_tag="PK522_01835"
     CDS             complement(22519..23277)
                     /gene="bioH"
                     /locus_tag="PK522_01835"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HNC40506.1"
                     /translation="MHRLHVESIGEGPDLVMLHGWAMHSGIWSSVQARLSDKFRLHLI
                     DLPGHGHSAQIPAFSLEQMVEVIATTLPSSCILCGWSLGGQIAIKLALRLPKQINKLV
                     LVATTPSFIKREHWQWGMDEATVQLFMSNLEKQYVQTINRFFTLQMKGSSDSSTALAQ
                     LRNNFFGSVKPNEQSLKVGLQILLTTDLREEVGNLTQPVLLIHGKNDVITDCEAAEWM
                     HQQLTDSKLDLYQRCGHAPFLSYPNQFVASLYDL"
     gene            complement(23317..24486)
                     /gene="bioF"
                     /locus_tag="PK522_01840"
     CDS             complement(23317..24486)
                     /gene="bioF"
                     /locus_tag="PK522_01840"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNC40507.1"
                     /translation="MLEELVRRLEQRKNDGLFRYRLTRQSPQTPHVKIATQSNLSFAS
                     NDYLGLANHPEVAAALCRGTLQYGVGSGASHLISGHSEVHHELENALAKFVELPNALL
                     FSTGYMANIAVVTTLVGHHDAVFADKLNHASLNDAALLSKAKLIRYPHLDLESLERAL
                     ARTNAPNKLVLTDAVFSMDGDIAPIKALLTLCQKHGAWLLVDDAHGFGVLGRAGKGTL
                     HRGLEPVDLNSPHLIYMATLGKAVGVFGAFVAGQPALIEMLIQFARTYGYTTAMPSAL
                     ACAIMQSLVLLEQEEWRREKLARLIALLKDHYQSSRWPLLASATPIQPLLIRESSTAI
                     LLSELLRREGILVPAIRPPTVPMGQARLRISLSASHHEEEVIRLAETLQRAERTI"
     gene            complement(24490..25485)
                     /gene="bioB"
                     /locus_tag="PK522_01845"
     CDS             complement(24490..25485)
                     /gene="bioB"
                     /locus_tag="PK522_01845"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041356864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNC40508.1"
                     /translation="MDSILSNIDQKEVNRIDHQQKEMRWNVEQIQILFDLPFNDLIYQ
                     AQSVHRQYHDANTLQLSTLISVKTGGCPEDCGYCPQAARYHTNVEDQDLLSTEEVVSA
                     AKIAKAEGATRFCMGAAWRGPKQRDIVKIAEIIRDVKALGLETCATLGLLKPGQAEQL
                     KQAGLDYYNHNLDTSPEYYQEIITTRDYSDRIHTLEQVRGAGINVCCGGIVGMGESRK
                     TRAALIAELANLDPYPESVPINYLVQVEGTPLYGTESLDLLEFVRTIAVARITMPKAM
                     VRLSAGRRMMPEAVQALCFLAGANSIFYGDKLLTTGNPAIESDKALFEKLGLRTV"
     gene            25580..26344
                     /locus_tag="PK522_01850"
     CDS             25580..26344
                     /locus_tag="PK522_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNC40509.1"
                     /translation="MSNSNTTLINNRTIIEQLLLPHRCVLCSIQQQGSLCQACKTSLP
                     KLDPNCCTLCLTPLPTFTHCGACLNTPPLWHYIVAGYRYAFPVDVMIQKLKYGLDLTL
                     TPILASFIVSKIDRNPLPDAIIPVPLHPEKMKARGFNQAIEISRYVSKQTGIRVLSDI
                     CLRVKNTPSQIELPWKKRQQNVRNAFRCQSDLTNKHIAILDDVMTSGATVNALAKEII
                     KQGATKVSVWVVARSLLSQKSQNNNQSLSQLSDEFL"
     gene            26331..26819
                     /gene="trmL"
                     /locus_tag="PK522_01855"
     CDS             26331..26819
                     /gene="trmL"
                     /locus_tag="PK522_01855"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HNC40510.1"
                     /translation="MSSFNIVLYQPEIPPNTGNIIRLCANTGSSLHLVKPFGFQLNDK
                     QLLRAGLDYHEFINLITHNNWEDCMRSFANKRIFAVSTKGKQRLDQVNFLKEDTFLFG
                     SESRGLPAEILASFPDERKIFIPMLPHRRSLNLSNAVAIIVYEAWRQIGYTAEESSAM
                     TN"
     gene            complement(26838..>27599)
                     /locus_tag="PK522_01860"
     CDS             complement(26838..>27599)
                     /locus_tag="PK522_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HNC40511.1"
                     /translation="IVEATETFSRTLGARFGVQNATQMGNRNLGISGNLDHSSGLASG
                     LLSPGGGQNLNVNLPASGLAAAAGGPAALGLSLIKINNGTLVNMELSALETDSRGRVI
                     ASPRLVTANRVEAVIEQGTEIPFQIVSQLGRPQIQFKKAVLSLRVTPQITPDDNVIMR
                     LRVNQDTRGQDTPAGPAIDTKQITTEVLVDNGGTVVIGGIFESADRLNRNQLPLLGDI
                     PVLGNLFKNSLRREDRRELLIFVTPRILNDNLSMR"
BASE COUNT         8404 a         6266 c         4990 g         7939 t
ORIGIN      
        1 atgtttgtgg atatctctat tttgccacat ttcaccacca gtgatctcag gaaaagcata
       61 aacacgctgc acatgggatc ccgtagtacc acccacttga tttgctgaag ctttttccgg
      121 atcaaataaa tacgggcctg ttatccgggt aactgtagga tcggtttcac tttggcgaat
      181 atagggacca cccgtattat acaaagctcc gcctaataca agatatcgat ctataattgg
      241 aagaaaaata ctgttatcgt aagtatgcgc tgaagcggga gcagcatcta caccgtcaat
      301 ggcagttgac gctgaagtcc ctgcaataac tgcaatctga ctcggtaacg aagcacgctc
      361 ccacttcaaa tcccgagacc gccaacggta gacatcgtta cctgaataat ttgcatgtcc
      421 accaccataa agaattaaat ctcctctatt actatcccat gcaaaactac tccatgctga
      481 aatcagttta cttggtgggg gattactaag aagattgagc ggccttaatt caggcggcgt
      541 ccagacctct gaaaaagaat ttgcattgac ttttttccat gtaccctcag gcatttgagt
      601 aattatttcg agcagaggac cctctgcata aggactaagc tctagcgctt ttagtggatt
      661 tatcaaaaac cacaagctca gaaaaaataa aaaacctcga aaatattttt gcttatattt
      721 ttctaactta ttcatttttt tacccttatt ctgttgtttt cttgataagc tagtgatatc
      781 tatgtgaaat gtcaacaagc cggagcaatt ttattgatct atagccagaa tatcagaata
      841 gtcaacgaat ttgatttttt tcactgcttt aactcaatgc gtatcaacta ccaatgatct
      901 tttttaggcg taatcgcttt gatgcctctg ctataaccca taaagctggc gttttaatcc
      961 acaccgatcc aaaataactg cgctgatttc ctcaatcgaa atcgcggtgg tatctaaata
     1021 gggaatattc attaatttaa acaatgattg ttgccattga atttcgtgct ggcattgtgc
     1081 gagtgaagca tactcactat tcggacggcg ctcttgtcta attaaatgaa gctgggctgc
     1141 gctgagcgtt aagccaaaca atttatcctg aacggattct agcactttgg gtaatcgcat
     1201 cgagcccata tcctcttgag tcagtggata atttgcagca gcgattccat actgcatcgc
     1261 taaataaagg caagttggtg tcttaccggt tcgcgatacc ccaatcaaaa ttatatctgc
     1321 tttagcaaaa tttttaggat taacgccatc atcatgcgcc aaaacatagt taatggcgtt
     1381 tattcttttg aaataagtca aatggtgctg aagcccgtga gaacgtccag caatccgcgc
     1441 aaaaggttga tttaattctt gctcgatcga acccataaac attgcaaaga aatcatagac
     1501 gcggcaattg gcttggcgaa cctgttccaa gatctctgga ttgagtaaag tactaaaaac
     1561 taaagggcga acaccctctt gcactgcaat ttgattgatt tgatcgatag ctactttgat
     1621 tttcccaatc tcatcaagaa atggaatgct aactttaacc cactcgattc catcaaattg
     1681 tgttaacaag ctatgaccta atgtttcagc ggtaatacct gttcggtcag aaagaaaaaa
     1741 aacagtacgc ctttgtttaa tcacacttaa ttttgttttg tcaggcttgc caaataaagc
     1801 caagtttcaa caatcgaatc gggatttaac gaaaggctac taataccaac ttcattaagc
     1861 caagcagcaa aatcaggata gtcagagggt ccctgcccac aaataccaat atacttgcct
     1921 tgagtccgac aagcatcgat ggccattttc agtatttttt taacggcagg atttcgttca
     1981 tcaaatgcgt gagcaactaa actcgaatcg cgatcaagtc caagacacag ttgtgtcata
     2041 tcatttgatc ctatcgaaaa cccatcaaag aactgtaaaa attcctcggc tagcaaagca
     2101 tttgaaggaa tttcgcacat catgattaca cgcaagccat ttctccctcg agaaagtcct
     2161 tgagatgcca ataatgctgt aacctgctgt gcttcttcta aagtacgaca aaaaggaatc
     2221 ataatttcca cattggttaa ccccatttca tcgcgaactt tgcgtaacgc ctggcattcc
     2281 aactcgaaac aagcttgaaa ctgcggtgaa atataacgag aagcaccacg gaaaccaatc
     2341 attgggtttt cctcttttgg ctcatagcgc tcaccaccga ttaaattcgc atactcattg
     2401 gatttaaaat cggatgtacg cacaatcact gggttaggat aaaaagcagc agcaagtgtc
     2461 gcaatacctt caaccaattt ttcaacataa aactgaacag ggtttgcgta tcccgcacta
     2521 cggccaagaa ttagtgcttg cagatcggat ggcaattgat caaattccaa cactgctttg
     2581 ggatgaatac caatcatatt gctgataatg aactctagac gcgctaatcc aataccttga
     2641 ttgggtattt gcgaaaatgc gaatgcatga gaaggattcc caacattcat catcatctta
     2701 attggtagtt gcggtaactg acccatatct gctaccgaat gttcgaatgc taataagcct
     2761 tcataaacat aaccggtatc cccttctgcg catgaaatcg taactgaact acccggtttt
     2821 atctgagaag tcgcctcacc acaacccaca acagccggaa tgcccatttc cctcgcaata
     2881 attgccgcat gacatgttct tccaccacgg ttcgtcacaa tcgctgcagc gcgcttcatg
     2941 ataggctccc aatcaggatc cgtcatatca gtgaccaata catcaccgga ttgaatctca
     3001 tgcatttgat cgatactgaa aattaccttc gctgttcctt gaccgatttt ttgcccaatt
     3061 gcgcgacctt ccgatagaac cattcctttt tgagttaagt gatatcgttc tagcgatgtg
     3121 ccagctcggc ttttaacggt ttctggtcta gcctgcacaa tatatagctt tccatctaac
     3181 ccatcgagcg cccattcgat atccataggc cttccataat gctgttcgat caataaggcc
     3241 tgctgaccca attgctcaat ctgttgatca gataatgaga atcgttgacg acgctcaaca
     3301 gaaacatccc gagttacagt aaatgtatcc tgcttgaagg aattctcata aaccatctcg
     3361 atcaatttag tgccaagccg tcgtgacaag attgcagatt taccagaacg cagcccttgt
     3421 ttataaacat aaaactcatc aggattgaca atgccctgca cgatcgtttc acccagtcca
     3481 taagccgatg taacaaaaat tgcatcttgg aaacccgatt ccgtatccaa agtaaacatg
     3541 acgccacttg cccccagatc actgcgcacc attttctgga tacccactga aagataaacc
     3601 ttatcatgag ggaaattttg gtgaacacga tatgcaatag cccgatcatt atagagcgat
     3661 gcaaacacga ttttgattgc ttgaataatt tgatctagcc cacttacatt cagtagcgtt
     3721 tcctgctgac ccgcaaatga agcatcggca agatcctcag cagtcgctga tgatcgaaca
     3781 gcaaatgaaa ctacttcacc gccagcgctt ttctcaagct tctcataggc aattgaaata
     3841 gcttctaata actgatccgg taacacagcc tcgatcaacc acgttcgaat agctttaccg
     3901 gtgattgcca acattgcaac atcatcgaca tctagttgat caagttcgtg tttgatacgc
     3961 tgactaagat cattcgcttc taaaaaatcc cggtaagctt cagtagtggt tgcaaatcct
     4021 gcgggaacat taactcccaa tttcgttaaa tgactataca tctctccaag cgatgcattt
     4081 ttccccccta caagggcaat atcgcgcata ctcaactcat caaaccactt aatatatgct
     4141 ttcatctcga atcatgacca gtcaattgat tgctcgtaat taataatctt atcactataa
     4201 gtataaatgg ggattgataa gggttcattt gaaacatttt gagacattga taagcttaag
     4261 tacttacaca ctcattacct aatttaaaag ttttgacatg ggtgaattaa agcgtagaat
     4321 aattttaaag gaacaagtta gtgaaattgg atcttgtagc agcatcatta ggtcaaaggt
     4381 tggcattatt ggcttatacc gatgtagcta gcttttgctt ctaacgcttc tcccctgcgg
     4441 tgttcgtgag ggctcaaatt ctttgaacca attcttgaaa tttggctgcg agctcgatta
     4501 atgctgttgt gatcgtcttt ttagacgaac ctgatgcaaa ctgcttgtgg ccaccttgga
     4561 cccaaaggtt caagatatcg gtctaacggc atctgcgggg ggctacctct caaaatattc
     4621 cttcagagta gtctggaatt tttaacagat agttgcttat aattaactca ttcaaatcat
     4681 ttaacttgaa gctaatgctt tagcagaaga taatggttta agttgtttat atcacctgaa
     4741 taccaaaaat taataatcta gcatagttaa ccttatgaat ttcatcaaaa ttaacttatt
     4801 gattcctttt acacttagtt tgtcttgtgc tttatggttt tctggctgtg taccgcttgt
     4861 cgcaaccggt gttggtgtag gcgctggtac gggcgctctg atgagtgaag atcgccgaag
     4921 tacaggtatt tttattgaag atgaggcaat cgaattaaaa acgagtcgaa gaattactca
     4981 gcaacttggt ggaaatgttc atatcaatgt aactagcttc aatcgaaatg tacttctcac
     5041 tggagaagcg ccaaacgagg caaccaaaag tgagattgaa aaacttgcgt tgagtattga
     5101 taatgttcgc aaggttttca atgaaatcac catagctgaa aatagcactt tatcatctag
     5161 aacgaatgat tcaatcatca cgtccaaggt aaaaggtcga tttgttagtg agagaaaatt
     5221 tcgggttaac tatgttaaag tggtgacaga aaatagtgtg gtctatctcc ttggtttggt
     5281 gactcgagaa gaaggtgaag atgcctcaca aatcgctagc acgacttctg gtgtgactcg
     5341 cgttgtaaaa gtgtttgaat acctaaatta gcattactga tcacgtcttc gctcatttat
     5401 tcagtgctac catctgagtt agttgcacaa atccaaaaaa ttattccttc ggaacgcttc
     5461 tacacggacc ctgtcgattg ctatgcctat ggatacgata atagtcgaaa aatttttccg
     5521 cctgaggctg ttgtttttcc aattactaca gatgaagttg tagcaatcgt ttcattatgt
     5581 aatcgataca aaataccaat tacaccaaga ggaagaggaa ctggaaccgc cggaggtagc
     5641 atacccgatc aggggggaat cgtattatca cttgaacgaa tggtaaaaat acttaacata
     5701 gacccaggaa atcgtgtcgt tgtctcagag ccaggcgtat taaatcaaga aattcaagat
     5761 acagtaaagc cttacggctt cttctggcca cctgatccat cgagtgctaa tttttcgagc
     5821 ataggcggaa atttggcaac aagcgcaggg ggccctcatg cggtaaagta tggaacgaca
     5881 cgcgatcacg ttttagggat aaaggcagta actgctactg gagatatcgt aaaaaccggc
     5941 tgctatacca ccaaaggagt ggttggctac gatttaactc gattactgat tggatcagag
     6001 ggaacactag ctgttattac tgaagccacg ctgaaattaa caccgttacc caaatgcaaa
     6061 agtggattag tcgcttattt tcaagatgcg accagctgtg ccaaagccat tgttgccatt
     6121 atgagtttac ctcaagggcc tagtgcactt gaatttttag ataaaaactc actaaatctt
     6181 attcggaatc gattccctga gctgatacca atcgaagcta ataccctgtt aatcattgaa
     6241 gttgatggtt cggaaaatga attatctgag gcgatggcta ctataaagtc tagctgtgat
     6301 attgatggga tattatcgat tgaacacagc aataatcccc atgatttatg gaaagctcgg
     6361 aaagcattat cacccctctt aagggatatc gcgcccaaaa aaatcaacga ggatattgta
     6421 gttccagtaa attcactacc tcaatttcta gatgatttag ataaaatctg taccaaattt
     6481 caaatagcga atgttaattt tgggcatgca ggtaatggca atatccatgt gaatctactg
     6541 atcgatccaa ataattcaga cgaaaattta cgcgcggaag cttgtctcga tgagatattt
     6601 gatttagtaa tcgcgctcaa agggacatta tcaggtgaac atggtattgg tagagaaaaa
     6661 cgcgctttta tacataagga gattgatgat cctacgctaa cgatcatgaa aagtatcaaa
     6721 accctatttg atccaaacaa tatactaaat cctggaaaaa tttttcccta atctagcggg
     6781 caacacttta cgttacggtg ataagcattt caggctataa aaaaacctta cacaagaatc
     6841 tatcttgtgt aaggttttct cgttaactag taatttttct ctacatcaga tataaaaata
     6901 atggtcaacg agtaatgctg taaataataa agccaagtaa agaattgaat atttaaacgc
     6961 tgcgcgtgct agctgatcac tgtaattgag ataaatcttt atcgcataat agaaaaatat
     7021 tgcatccaaa actattgcac taaataaata gatcaaccca ctcatctgcg aaacataagg
     7081 cagaatcgtc acaacacaca aaatcaaggt ataaagcaaa acgtggaggc gggtaaattt
     7141 atcgccatga gttaccggta gcatcggcat accaatttgc gcatattccg ttttgcgata
     7201 cagtgctaaa gcccagaaat gcggaggtgt ccaagcaaaa ataattaaaa acagcaatag
     7261 agcatctgcc gttacttccc ctgttacagc agcccagcct aagacaggag gcatagcacc
     7321 tgaggcacca ccaatcacaa tattctgagg cgtaagtggc tttaagataa cagtgtaaat
     7381 tattgcatag ccaacaaacg tacctaatgt tagccacatt gttagtggat ttatccattg
     7441 atgcaagata aataacccta acccaccaat taatccaaga aaaaataaag tctcgggaac
     7501 acttaccttt ccttgcggca gtggtcttcc tcttgttcta gccataaccg catcgaattt
     7561 ttgttcaact aaacaattta gtgcagcggc agcaccggca acaaaagcaa ttccaattgt
     7621 tccaagcact aaggtatcaa gtggtactgc acccggaact gctagaaaca ttcctattac
     7681 agcagtaaaa acgatcaacg aaacaacacg aggtttcgtt aaacggtaaa attgctggag
     7741 tctatctaaa actgactggc ttgtacttgt gttaacactc atatcttata ttaccctcct
     7801 aaaatacaat tcaaattact tatgttctaa atgagatagt ttcaataaca gtttaatatc
     7861 atttaccatt tttgatggat tagcatcccg agggtaacgc atcattaaat ttcccatagg
     7921 atcaatgaca tagatatgat cttttcttga agaaacggct ggaaattgat ccagaaactt
     7981 attatcgtta acagttaata atactgttcc ttcaaattct tttttgattt cttgatttgg
     8041 tactatttga tcatcaataa accaaattct atttattctg tctttctcag tattctgggc
     8101 taaacgtact tgtcgcattt gatacaattt tttttgacaa tactcttcac agttgccgga
     8161 gtcgatcata gcaaaagacc acttcccatg taattgtctt tctcgaaata tcgtattatt
     8221 ttcaatctca tgcgcttggc catgtattgc ctttacttct agcaattctc catagttaac
     8281 agtgctatca gggcgaaatt taatattgtg caaaatcact gacgccatta cgggcgatag
     8341 cataagcaat atcaatgcta taaattttgt cctattgctg cgggctactt cgtttgacat
     8401 ttaatgctaa taataaaata aaaacagtaa ttgctaaaaa gaaccattgg aatgcataac
     8461 tcatgttttt agaagcacct aaatcttgtc gttctagctc acgtatcaat ccatcattaa
     8521 ttgaattatc gagctgcatg agcagaatgg gttgtagttc taacttactg gctgcctgat
     8581 attcttcaaa attaaacgct aaccatacct taccggtaga aatttcatcg gaaattttca
     8641 tcgtcctgat tgttggagaa agagcgatgc cagaaagggt aattacatcc gttggcgtaa
     8701 aaatttcagg caatgtagat cggtttttac caagtgccac ccagccacga ttaattaaca
     8761 catgcatatt actattttca attcgcattg gtgttaacac gtgatatcca acagcacctc
     8821 gataaacttt gttatcaagg aatattgtat ggcttgcaat aaattgaccc cttacttcta
     8881 cattccgaaa taatagatcg ctcaactcaa cccgcgaaga aggcagtgtt aaaactgctt
     8941 catgtgctaa ctgatctagt aattgctgct tcgaatcttt ttcatctgca cgagaaagct
     9001 gccacagacc taatcgtgta aagataataa ttaatattac tgataaaact agtatccacc
     9061 attttggttt gaactgataa tctttgataa tcattcatca atcaacttct aggcttctac
     9121 ttataattat tctctttttc tatttttata atcatactga tatcgctatc agtatgatgt
     9181 attttcctta cttcacaaca tttattagta cttataacaa atacacaaat ataaataaac
     9241 ctaaccaaac tacgtcaaca aagtgccagt accaggccac accctcaaat ccaaaatggt
     9301 gctctggctt aaagtgacct gctgcacatc tgaaatagat gactatcaac ataatagtac
     9361 cgactgttac atggaaacca tgaaatccag ttaacatgaa gaaggtagac ccataagcac
     9421 ctgtcgtcaa tttaagattt aaatctgcat atgcatgacc atattcataa atctggcagc
     9481 ataagaatgc aataccaagt gcaaccgttg ctaacatcca tttttttaag ccactgcgat
     9541 catttttctt aagcgcccaa tgagcataag taatcgtcac acctgaagtc aatagcagca
     9601 tcgtattaat tgctggcaaa ccccaagcac ccattggtgt gaatgcctcc gtaattccag
     9661 gacctgctgt tggccaacct ccagtatatc ccggccatat caactgatgc tccatgtccg
     9721 ataaccaagg taccgaaagc tgtctcatgt agtaaagtgc gccgaagaag gctgcaaaga
     9781 acattacttc agaaaagata aaccagctca ttccccaacg aaatgattta tcttcttgtt
     9841 taccaaattt tcccgattca ctttctctag caacctcacc aaaccatcca aataacaaat
     9901 aagttagtat ggcaaaacca attgctagca tccaataacc cgattcaatc ttgttcatgg
     9961 taaatgcagc accaaaccct aaaaatagca gtgctgacga ccctacaatg ggccatttgg
    10021 atggtgacgg tacataataa tgaccggttc cgtgactcat atcattcctc ctgaatttat
    10081 tttaatcaac tagcttgtta cccatttaac tactaataag atgctgacta caaaaaacaa
    10141 tccaccaatc aatcccccga ttactacttg actaggctga atcatttctg catctttttc
    10201 taaatcactt ttttttctta ttccaacaaa cgaccaaaac actgctttaa caacttgcca
    10261 caatgaagct ttctctgatt tctgcattta taagttcatc caaatttact tttcctattc
    10321 taatttaccg cgccaagcga gaaaaaagta taagaaattg tcatcgttgt tatatctgca
    10381 ggaatctgtg ggtcaattac aaatttaact ggcatttgtc tagtttcacc tggttttagc
    10441 acttgctttg tgaaacaaaa acattcaaat ttcttcaaat acttttcaag aacgcgcgga
    10501 ctataactgg gtatcgcctg accacttgtt tcttgatctg aattgttact aatttcatac
    10561 attacctcga tggattcacc aggatgaatt ctaatattcg actgtaacgc cttaaactgc
    10621 cagggcaatc cacgtacatt tgaatctagt tgaatcatta ccagacgtgt tgtatcgacc
    10681 tttatgtctt tatcagccac atctggtttc agtagattat tgataccggc aacttcacaa
    10741 aatttctcgt aaaacggtac cagcgcgtaa ccgaaggcaa acataatcac tgagaaaacg
    10801 agtaattttc tcaacattgc tatattaatc ttggctacgt tatacatcat tgcttgatta
    10861 gaacagttac agtaaatatc aaaaccatca ttgccaatag aaaaaacgct gttttaacat
    10921 ttctccaaat gtgctttcta tctttatcat cctgtgaatc catactctcc tcacattaat
    10981 tacttgatgg taggaggtgt ttcaaagcta tgataaggtg ctggtgacgg taatgtccat
    11041 tctaaggttg ttccaccttc ccatggttta tcagcagctt tctctccact ccgaatacac
    11101 ttgattacga tatacagaaa gaatagctga gttaagccaa atccaaaacc accaatgctt
    11161 gataccatat taaaatcagc aaactgcaaa gcgtagtcag gaactctgcg tggcatgcct
    11221 gctaatccta agaaatgttg cacaaagaaa gtcaagttga agaagaacat ggataaccag
    11281 aaatgccatt tacccaattt ttcatcatac atacgaccac accatttagg taaccaataa
    11341 taaacagcac caaagattgc aaatagcgca cctgatacaa gcacatagtg gaaatgtgca
    11401 attacatagt aggtttcttg aacttgaata tcgattggca cgattgcaca aatcacaccg
    11461 ctaaaaccac caattacaaa taagaagata aatccaatcg aaaacagcat tggcacttca
    11521 aaagtcattg aaccacgcca cattgttgcc gtccagttaa atactttcac accagttggc
    11581 acagcaatca acatagttgc atacatgaag aatagctgac cgactgatgg catgcccact
    11641 gtaaacatgt gatgcgccca aacgacacaa gataaaattg caatcgaagc cgttgcataa
    11701 accattgaag aatatccaaa caatggttta cgagagaatg ttggaatgat ttcagataca
    11761 accccaaatg ctggcagaat catgatatac acttctggat gaccaaagaa ccagaaaata
    11821 tgctggaata atacaggatc accaccacct gccgcattga agaaacttgt accaaagtta
    11881 cggtctgtta acagcatggt gacagttcca gctaacacag gcatcaccaa caccagcaaa
    11941 tatgccgtaa ttaaccaagt ccagacaaat aatggcattt tcatcaaggt catccctggc
    12001 gcgcgcatat tcaatatcgt tgtgatgata ttaatagcgc ccatgattga tgatgcgccc
    12061 aacaagtgaa ccgcaaaaat catcatatca acgcccatac caccttgagt cgacagtggc
    12121 ggatagaatg tccaacctcc agcagcagca ccgccaggaa caaagaagga gcttactaac
    12181 aaagtagccg ccacaggcag aatccagaaa ctccagttgt tcatacgagc aaatgccata
    12241 tcaggcgcac caatcatcat tggtacttgc cagtttgcca accctacaaa cgcaggcatg
    12301 atggcaccga ataccataat taacccatgc aatgtggtaa aagagttaaa cagctcaggc
    12361 tctagaattt gaatacccgg cataaataac tcggctctta tccccattgc caagaatcca
    12421 cctaccaaaa acattgcaaa actgaaccat agatacatgg ttccgatgtc tttatggttt
    12481 gttgtacaaa cccaacgcat tatccccgtt gggtgatggt catcatgacc atgaccatga
    12541 acgtcttgaa ctactgccat gatttactct ccttagtgtt tctactattt atattacaaa
    12601 aaaactaaat aattaattta aagccgctgt tgtcatcgcc gatgctgaag ctttttgttt
    12661 atcagctacc cactgagcat atttttctgg ctcaaccacc tcaacaacaa tcggcatgaa
    12721 accatgatct tttccacaca attcagtaca ctgcccacgg aatatccctg tccgatcagc
    12781 cttaaaccag gcatcacgca caaatcctgg aattgcatct tgcttaacac taaacgcggg
    12841 aacccaccaa gcatgaatta catcattagc cgttacaatc acccttactt tcttcccaac
    12901 cggcacaacg agattattat ccacttctaa caaataattt tcacctttcg gttctttatt
    12961 ctcaatttgc gctcttggtg tagataattt actgtaaaag cttattcctt caccttcacc
    13021 ttggagataa tcatagcccc acatccattg atagccagta actttgatag ttatgtctgg
    13081 ctctgaagtg tctttcatcg caagtactgt ttttgttgct ggcaaagcca ttacaaccaa
    13141 aattgcacaa ggaattgctg tccagataat ctcaactaaa gtgctgtgat gaaaattggc
    13201 cgctttatac cctaaagatt tacggtgctt cagaattgaa taaaacatta cgccgaaaac
    13261 cgcaataaaa atcgccaagc aaatccacat cgcaaatata tgctgatcat agacatcttg
    13321 agcaattgcc gattgcggct ctggaaaatt atatttactg gacatcgcta agctcgagta
    13381 aaaagtaaga agcgccgccc ccactcgagt tgctactgat ttactactca tatattcccc
    13441 cccacatatt aatacaaact tacttgtcta ttttgacaaa ccaaaaactt taaaactctt
    13501 aatagctaat tcttttaatt accttaactc aaagtaaatt tagctataaa actaaaacaa
    13561 agttatctat caagatatct agatagtttt tttaccagac taccattctg aaccaggtta
    13621 agattctatc atgctgagta tgctcaaaca agggaaaatc atgattttta tagggaaatt
    13681 accctatcaa aattagggta atttccaaat tagcagcaca ctaaaattag ataatgagaa
    13741 aaaaacaaaa tgcgaatctg gtcaaaataa ataccaagca cttattttgc agaagagatt
    13801 gatgacttat ttagataaaa ataaataagt tattctcata taagaataat tagaaatttt
    13861 caatagataa ctgcttatca tcagattttt gagctaattt tcgcattttc aaatcgcctc
    13921 catcccaata accttatgga aattgtggca attgtaaaga taatgattta tttaatgcaa
    13981 acagctctta tttgatgaat atcagtaatt ccttgcaata ctttttcaat gccatcttgc
    14041 tttagcgtgc gcattccttc acttagcgcg gtaactaaca tatctgcaac acgcgcatgc
    14101 tcctgaatat tttttttcaa cgcatcactg gcggttagca gctcatgcaa accgaccctg
    14161 ccagaatacc cggtcccatt gcaactatca caaccgactg actgataaag cgtaatttgc
    14221 ccttgctcat taccatattg cttaatccaa tccccctcaa tccgtttgta agcagcgtca
    14281 ggatttgttc taaagcgctc aatattcttg agctcttcac agtattccgc taaaagtgac
    14341 tgaatttcat cgcttcctgc cgtgtggggc tttttgcatt tactgcataa ccgctttgcc
    14401 aggcgttgcg ctaatatgcc caatagtgca tctgaaaaat taaatggatc cattcccata
    14461 tctagcaaac gaataattga ctctggtgcg ctgtttgtat gtaacgtagc aaaaaccaag
    14521 tgcccggtca gtgaagcctc aatcccgata ctcgttgttt ctttatctcg catctcaccg
    14581 accataataa tgtcgggatc tgcacgcaag aatgatttca tcacaactgg aaaagtcaaa
    14641 cctgctttga cattcatctg aacttgcctc aatcctttct gggtaatttc aaccggatcc
    14701 tctgctgtcc aaatttttgt ttcagatgta ttcaaatatt ttaggattga atgtaacgtt
    14761 gtagtcttcc ctgaaccagt cggtccacaa acaaaaaata aaccataggg tttactgata
    14821 attgtcttaa gttcatcaag attacgtggc gtaaagccca ttttttcaag tggtatcggt
    14881 tcacctgcag caagaatacg catcactaca tcttctaagc cgcctgcaga aggtatcgtt
    14941 gcaaccctca actcaatatc aagtggcgca aatttcttaa attttatttt tccgtcttga
    15001 ggttttcttt tctctgaaat atctaaatca cacataatct tgatacgcgt taccaacgga
    15061 ttacgataac ttgcaggtat ctcaatataa ggcattaatg aaccatcttt gcgaaagcgg
    15121 atttttgttt tctcttttcc tggatatggt tcgatatgta tatctgaaac ccccattcga
    15181 tgcgcatcca taataatttt gttgaccaac ttgacaagtt cattatccga tgcagctgat
    15241 gtctcatccg tttcaacaac aacctcttct tctccctcct tgagatcaaa aagcatatcg
    15301 ttgatattgc ctgtatctac aaggtttgta ctaccgactc ccccataaaa caaatcaagc
    15361 gttgctttaa attctgggtg cgtacatacc gtgtaaacta tcttatgctt aggaaaaacc
    15421 tgattaacca tccggagcac tttgattctt tccggatcta gcgcaagcac tatgagacct
    15481 tcttttgcgt catcaatagg aagccactga ttactctcca caaaatcacg tttaagattt
    15541 ttcagaatct ccatcggctt gattcgatca gctcgataag gctcataggg taccttaaaa
    15601 tacaaagata acgcttcgcc aatctgtgaa ggtttaattt gaaactcatc aattaaaata
    15661 tcttcaaggt cacagccttt ctctcgagca ttccttgcag ccatttccag ctctgcagct
    15721 gaaataattg aatctgaaat caagtgatca tattttgaac gagccaagta ttgttgcttc
    15781 tgcctttgtt taaaagccac tgctaatatt tggcaaatct cacttatgcc cgcaactacg
    15841 atctgtgaaa acggcttatc ctctttattg ttaattacct gaacaattcc gataagctca
    15901 ttatcgccca cgtttaaaat cggagctgct agcatttgct tggttcgata accggttcgc
    15961 ttatcaacct cttttaaaaa gtggagttgt gagctgagtt tcatcaactc accacgatca
    16021 tatacatctg ctatattaac aattttttta tttaggccag caaagccagc gatactctgt
    16081 tcggtgatcg gtaatttcaa gtcttggaat gaatctaatc ccgttttgac ccgtgaaaca
    16141 atgtaagact tatcctcact gatcgtataa atcgtcagcc gatccgcatt aaacaacgcg
    16201 caaatatctt tgctgacttc aagcatgatc tcatcgatat tattagcagc gttgattctt
    16261 gccgcaactg cttgcaaacg tttagagaag tctaattgat aattgatatc taaattctgg
    16321 ccagatactg gctctagcgt aaccggtctg ttcatggttg ctttatccaa agtgctaaaa
    16381 ttcaaaaatc tgattactca ttaatcggcg tgaattaaat cgccctgcac atctttcaat
    16441 ttcttgcatc gttagatcag cctcgccttg gcgcagatga gttataaaga tttccacctc
    16501 ttgttctaat ttttccaact cttcagctaa catgcttggg cataaatgtt ttgatcgcat
    16561 tgcgatatct ttcttcgcat tacaaaacgc aacctcaata attagatatt tcaagttttg
    16621 aatcttattt acctctaccc ataacgcatc atttgtagtg gtatcaccgg taaaaactaa
    16681 actagccata ccagaatcta aacggaatcc aactgccggt acaacgtgat tagcgggtag
    16741 tggcgtaatc ttccttccct ctaaatcaac ttcatttcca attgctaaag atttgtaagt
    16801 taaatagggg ttttcaccat taggaatttt ggtaaagtca ggccaaattt cccaattaaa
    16861 aagatgtttc tgaattgctt ctagcgtagg aggtatggca tgaacaatca gtggaaactc
    16921 tcgcaaacaa cctactgtgt caataagaaa aggaatcagt gcgatatgat ctaagtgaga
    16981 gtgcgtaaca aagatgtgtt taatttttac caattcatcc aaagtcaaat tgctaacacc
    17041 cgttcccgca tcaattagta tgtcatcatc aagcaacatt gatgtagtac ttgatccttt
    17101 gcctatccct ccactacagc ctaatactct aagcttcaaa atatatcccc cttattcgaa
    17161 tatttaacct gatttttaga tatttgcttt cgataataag cagggaatta cattggattc
    17221 taattatttg actaacagaa ctggaatatc agacagatgg atcagtttgc tagcaataga
    17281 acccagtaac atacctgtta ctacgcctaa tccccttggt ccaataacaa tttgatcaca
    17341 ttgctgtgct tgggcaaatt gaataatggt ttcagcaggt tctccaacag caacatgata
    17401 ttggtacgcg acttttgctg tatccaacaa tttacgcact tgcatcaaat cctttaatcc
    17461 ctcttcttga tgatattgtt tgatattgtc ttgactaata aacattgaaa tatcaccttg
    17521 caatggatat tgcacgttca gtaaatgaat ggtcggtagt tccttatacc atccaagaat
    17581 agagataaat tgcgaaattg ctttactaca agcctcagaa ccatccacgg gtaacagcat
    17641 ttttaacatg acatttcctc aaatgttgtg tgatttttca cagaatccaa aatcatcaaa
    17701 tcgatttatt attatcacca agattcgtta ataccattgc cttcctgact gcacgggctg
    17761 ccagccattt tccagtaata attacaatca atgtcgttac aatcggtgca acccaacgca
    17821 gatacgcggc atgatcttct atccaaactt tggtaacagt atccgttgtt gacatatcac
    17881 ctgcaatcca acctagcaaa ccagccccaa tcacaatagt tacaggaaac cgagtcatca
    17941 acttcataac gaatttactt ccccaaacta tgatgggcac actgataagt aatccaaaaa
    18001 caacaagacc aatatcaccc tttgcagcgc ctgcaatgcc aattacattg tccaaactca
    18061 ttacagtgtc tgcgataatg atcgttttaa tagcgccaag caaagttgtc ccagcatcga
    18121 cttgatgctc attatccgaa gactctggca tcaataactt aataccaatc catatcaata
    18181 gtaatgcgcc aacaattttt aaaaagggca gcgtcaaaag attcagcgca aaaaatacta
    18241 ataggacacg tataaaaatc gccccaaaaa caccccagaa gattccttga ttacgttgtt
    18301 tttctggtaa tttgcggcag gccaaggcaa taattaccgc attatccccg cctagcacaa
    18361 tatcaattgc aataatttgt agtgccgcga cccaaaattc gggctcataa attgtcattt
    18421 tgttatttcc ttcagattat tgtatgaatt taagaatctc agttcttaac tgcattgcat
    18481 cacggtaccc taaatcgatt aaagcgctac aaaaagaagc ttcaaataaa acatagctta
    18541 ataacccaga cccactagaa cccattgcac ctaccgcacg atagaggtag cgcattgtat
    18601 gcggaagctg atgcgcatgt tgcagtgcaa tctcattgat cctttggcta ggcgcaatca
    18661 ccatcgactg aatgggtcgc aatgtcagac tgctttcctt tctagcattt ggaggaatga
    18721 gtgcgagcgt ctcattaatt ctattcaaac gttctaaatc aacatttaaa ctatcagcaa
    18781 agatactgtt cattgcatgt ccggctattt gggcgagcgg tgggtagctc gtgactttga
    18841 tccgttcact agtaacaata tctttggggt taacaccgat cacgagcacg cgatcagcgc
    18901 ctaaatgcaa tgcaggacta agcggtgcaa actgacgcat ggaaccatcc ccaaaatact
    18961 ctcgatttac ccgcacggct ggaaagataa atggaattga tgaagaagcg agcaagtgct
    19021 gaatgccaat ataggaagcc acgcctacgc gttgcgaacg tttccaagga agaatacttt
    19081 tgtttccctg aaaaaacgta acggattgcc ctgaagtata tccccatacg gtaatcccta
    19141 gtgcatgcaa ggctcctgca tggatacttc tctgaatacc cctaaatggg agccgacaag
    19201 ccaacattga tcgtaatgga gcattatcca atagcgatac gggtctccga gctaatccac
    19261 tcgaaaataa agaacctaat aaatgaaggg tatttcgtat caatcccagc aaatctgcac
    19321 gatgcaccat gtcaacatgc aaattacacc aaactttctg cagttgcctg accccagttg
    19381 agaaatgtgt cgctgacatg gccaaacctg ctgcattaat tgctccagca gaagtgccac
    19441 agatgatgga aaatgggcta cgcgcatgcg ccggaaccat ttcagcgata gcaagcagta
    19501 ccccaacctg gtaagcagcg cgcgcgccgc cacccgtcaa tacaaggcca attttgggta
    19561 atggattagt ggtagtggta tcgtggtcac tcacagctga tgaatttaga atttaccttt
    19621 tcaagtgcat cggataatgt ttcctcaggc aaatcattca agctagctga taataccttc
    19681 gccgatttac aaatatcgcc taataatatt ttatcatcaa gatttgcaat aaatactgct
    19741 gctgcaccta ctgtccttaa aaggctttgt cgctcactca ttagcatctc tatctctgag
    19801 cgtaacattg ataattcctg ctcttgtcgt tgtgtatgta tcataaatta cttcctatgc
    19861 ctggtatact aagatgaact taacaacaaa ctataacagt atcttctatc ccttaaacag
    19921 ccaagctctt tggaatttaa tcgtcttttc gaattccttt gaaatcgatc accaccaacg
    19981 aacaatttaa taacaacctt aaatgaagct atttgaacta tacccggaaa ttgagcctta
    20041 taatcagggc atgttaccgc ttgacaccat gcatactatg tattgggaac aatctggtaa
    20101 cccatctggc atccctgttt tatttttgca tgggggccct ggtgcaggat ccacatcaca
    20161 tcatcgccgc ttcttcgatc cagcgagtta tcaaattgtt atctatgatc aacggggtgc
    20221 tggcaaatcc attcccctcg gtgaaatccg taacaacact acgccccatt taattgaaga
    20281 tattgaagca ctccggaagc atcttggtat tgaccgctgg ttgatcttcg gcggatcatg
    20341 gggaagcact ttagcgctag cctacggaga aagctatcct gatcgctgct tgggctttat
    20401 tttacgagga attttcttat gccggaaaag tgaaatagat tggttcctct atgggttacg
    20461 cactctcttt ccggaaccct gggaagaatt tgtatcggaa ttggctccat cggaacaaaa
    20521 agatatactg gctacctatt atacgcgtct aatggatctt gatcctgcta ttcatatgcc
    20581 tgcggcccga gcttggggac gctatgaggg tcgttgctcc actttacttc caagctcaga
    20641 aacagtaagt tatttctcaa gtgacggtgt tgcgttaggg ttggcacgta tggaagcaca
    20701 ctacttcaaa cataatatct ttctgcctga aaactcatta ctcgacaata taagcaagct
    20761 ccaacaccac cctgcaatca ttgtacaagg tcgatatgat gcggtttgtc caatcgttag
    20821 cgcaaatgat ttgcatcatg cctggcctag tgccgagtac ctcattattg atgatgcagg
    20881 acattctgcc tgggaactag ggattcgatc cgcattagtt gaagcaacag aaaaattcaa
    20941 atcaatcgat cgataatatt tgattgcttc acattagaag cgggtttata tccaacaaat
    21001 ttgcagatac actcgcatca atatgcgggt tatagggtaa tatcccaatt aatgggcatg
    21061 ccagtctgcg cctcagcact tcaatattct ctgtaatatc atccatttgg gggtcaatca
    21121 tattggccac ccatcctact aaaggtaaac gtcttgcacg aatcgcttca gcagtcaaca
    21181 aagcatgatt caaacaaccg agacgcaatc ccaccaccat aacgaccggt aaatttaatg
    21241 cgaccgccag atcactcgta tccgtctgag aagttaacgg aacgacaaac ccaccgacgc
    21301 cttcgacgat aacgatatct accgctttct gcacaatatg aaatgcttgt tgaatcactg
    21361 caatatcgat atcaaccgct tcataatttg ctgcaatttg aggtgcaact ggcgccaata
    21421 aatgataaga gttaatcata gcgcgatcaa attgccttgt agatgctgca cttagctgat
    21481 cgacatcaag ccatcgacca ttttcgcaac cggctgctat cggtttcata ccgacaaccg
    21541 atcgcctcgt ttttgcaaaa gcatgaataa gcgagcagct aaccagtgtt tttcctacat
    21601 tggtatcagt tcctgtcaca aaaaaaccaa ttgacattac actattattc gctgattccg
    21661 agtttttgtt ttgttgtagc tttaagcaaa gctaattttg attcaggttt ccaggcatga
    21721 ccataaacca cttcatacgt cgcaggcaat ttaccagatt ggcgtagcaa ttcataacga
    21781 tcgatgatct gctgccatgc tctttttccc atgaggcctt ttttacgccc aatcataaca
    21841 ttatgggcgc caatcgcttt gaggtcacgc atgatactag ccacatcttg ataagtcagc
    21901 gtaatgtatt ccatatccat gactggttcg gcaaaaccgg catgaactaa tatatcacca
    21961 atatcatgca tgtcgataaa acgattaatg tgttggtatg aatcgaccgc tttgaaggtt
    22021 tttctcaatt cggataaggt atcgggaccg aaagtactga acatgatcaa tccacccggc
    22081 ttcaagacac gatgacactc cgtcatggat tgctttaaat cattgcacca ctgcagcgcc
    22141 aaattcgacc aaatcatatt aacggattct ttctgtaagg gtaattgttc aatatcacca
    22201 gttacataat cgatagaatg gcgttttacc agtgaaatcc atttcctcca ccctgaaatt
    22261 ggtcggtaag cttgcaaatg catatttaaa gcaatatcaa gtgcaataat tgtagaacga
    22321 tcatagcgct ctcgtagctt ttgagtacca taaccagtcc cactccccat gtctaagata
    22381 aaatcaggaa gatatttaat gaaatcgagc ctagataaca ttcggtcaca tatttcacgc
    22441 tgtaaaaccg cagcgtgatc atacgtctca gctgcttttt caaatgatcg tcgcagtaaa
    22501 cgcttatcta aactatgttc ataaatcata caagctagct acaaactgat tagggtaaga
    22561 taaaaaggga gcatgcccac aacgctgata taaatccaat tttgaatcgg tcagctgttg
    22621 atgcatccat tctgcagcct cacaatcggt gattacatca ttttttccat gaattaatag
    22681 aacaggttgc gttaaattgc ctacttcttc acggagatcg gtggttaata aaatttgtag
    22741 tcccacttta agcgattgtt cattaggttt cacagatcca aaaaaattat ttctgagctg
    22801 cgctagtgcc gtcgatgaat cggagctccc tttcatttgc aaagtaaaga accgattgat
    22861 ggtttgaaca tattgctttt ctaagttgct cataaaaagt tgaactgtcg cttcatccat
    22921 tccccattgc caatgttcgc gttttatgaa gctgggtgtc gtagcaacta aaactaattt
    22981 attaatttgc ttgggtaaac gtaaagcaag tttaatcgca atctgtccgc ctaatgacca
    23041 accacataaa atgcatgatg aaggcagcgt tgttgcaatg acttcaacca tttgttcaag
    23101 cgaaaaagca ggtatctgcg cactatggcc gtgccctggt aaatcgatta agtgcaaacg
    23161 aaatttatcg gagagccttg cctgcacact actccagata ccgctatgca tggcccaacc
    23221 atgtagcatc acaagatctg gcccttctcc aatcgattcc acatgcaaac gatgcataac
    23281 ttattactac ttaccttatt ctcatgctga tcttatttaa atcgttcgct ccgcacgctg
    23341 caatgtttca gccaagcgaa tgacctcttc ttcgtgatga ctcgctgata gggaaatacg
    23401 caaacgagcc tgacccattg gaaccgttgg cggtcgaatc gctggaacca gtatcccttc
    23461 tctccgcagt agctcactta acaaaatcgc cgtactactt tccctaatta acaatggttg
    23521 aatgggtgtt gccgaagcta gcaatggcca acgagaagat tgataatggt cctttaataa
    23581 ggcaatgagt cgagcaagtt tctctcgcct ccattcttct tgctccagca aaaccaggct
    23641 ctgcatgatt gcacaagcca gtgcagaagg cattgctgtg gtatatccat aagtacgtgc
    23701 aaactggatc aacatttcga ttaatgcagg ctgaccggct acaaatgcac cgaaaacccc
    23761 tactgcttta cccagtgtcg ccatatagat gagatggggc gagtttaaat caaccggctc
    23821 caggccacga tggagtgttc cctttccagc acgacccaac accccgaaac catgcgcatc
    23881 atcaaccaat aaccaagctc catgtttctg acagagcgtt agcaacgctt taatcggtgc
    23941 aatatctcca tccatactaa atacagcatc agttagtacg agcttgttag gagcatttgt
    24001 tcgagcaagc gctcgttcaa ggctttctaa atcgagatgg ggataccgaa tcaactttgc
    24061 tttagaaagc aatgcggcat cgttaagtga cgcatggttc agcttgtctg caaatactgc
    24121 atcatggtgc cctactaagg tcgttacaac ggcgatattg gccatataac cggtagaaaa
    24181 taatagtgca ttgggtaact cgacaaactt agctagtgca ttctccagtt catggtgaac
    24241 ctccgagtgc ccgctgatca aatgcgaagc accggaaccc acaccatatt gaagcgtgcc
    24301 tcgacaaagc gccgcagcga cttctggatg attagccaac cctaaataat cattactagc
    24361 gaaagaaaga ttagattgcg ttgctatttt tacatggggt gtttgtggac tttgtcgcgt
    24421 caaacgatag cgaaacaatc catcattttt gcgctgctct agccttcgaa ccagttcttc
    24481 aagcattgcc taaacagtgc gcaagcctaa tttctcaaat agcgctttat ccgattcgat
    24541 ggctggattt ccagtggtta ataatttatc cccataaaaa atagaattag cgccagccag
    24601 aaaacaaagt gcctgaaccg cttccggcat catacgtcga cccgctgata gcctcaccat
    24661 cgccttaggc atggtgattc gcgcaacggc aatggtacga acaaattcta ataaatcgag
    24721 cgactccgta ccataaagcg gtgttccctc aacctgtacg agataattaa taggcaccga
    24781 ctccgggtaa ggatcaaggt ttgccaattc agcaattaat gccgcacggg ttttgcggga
    24841 ttcccccatt ccgacaattc caccgcaaca aacattgatc cctgctcccc tgacctgctc
    24901 cagtgtgtgg attcgatcac tatagtcacg tgttgtaata atttcttgat agtactcggg
    24961 cgaggtatct aaattatgat tgtaataatc gagaccagcc tgcttgagtt gttcggcttg
    25021 acctggtttg agcaagccta atgtagcgca ggtttcaagc ccaagtgctt taacatcgcg
    25081 aattatttca gctattttaa ctatatcgcg ttgtttaggt cctctccaag cggctcccat
    25141 acaaaatcgt gtagcgccct ctgctttggc aatttttgcg gccgaaacca cttcctccgt
    25201 tgaaagcagg tcttgatctt caacattggt atgatagcgc gcagcttggg gacagtaccc
    25261 acagtcctca gggcaccccc ctgttttaac tgatatcaaa gttgaaagct ggagtgtgtt
    25321 agcatcgtga tattggcgat gaacagactg cgcctgatag attaaatcat taaagggtaa
    25381 atcaaataga atttgaattt gttcgacgtt ccatcgcatc tctttctgct gatgatcgat
    25441 tcgattgacc tctttttgat caatattact taaaatggaa tccatactat aaataaatca
    25501 gttaataaat gactaagaat tctcttatgt tagcgggtaa gaattgaatt gcaccgaatc
    25561 atcacggatt aaacactgat tgtcaaattc taacactaca ttaattaaca accgaacaat
    25621 aattgagcaa ttattgttgc ctcatcggtg tgtattatgc tcgattcagc aacaaggcag
    25681 cctatgccaa gcatgcaaaa caagcctacc taaactcgat ccaaactgct gtacactttg
    25741 cttaactcct ttaccaactt ttacgcactg tggagcttgt ctcaatacac caccactgtg
    25801 gcactatatt gtcgcagggt atcgttatgc ctttccagtt gatgttatga ttcaaaaact
    25861 caaatatgga ttggatctta ccttaacacc tattttggct agttttattg tatcgaagat
    25921 cgatcgcaat ccacttccag atgccatcat tccggttcca cttcatcctg agaaaatgaa
    25981 agctagagga ttcaatcagg ctattgagat tagtcgctac gtctctaaac aaaccggtat
    26041 tcgagttctc tccgacattt gtttacgtgt caaaaataca ccttcacaaa tcgaattacc
    26101 ctggaaaaag agacaacaaa atgttcggaa tgcttttcgg tgtcaatccg atctaacgaa
    26161 taaacatatc gctatattgg atgacgtaat gacaagcggc gctacagtta acgctttagc
    26221 caaagagata atcaagcaag gcgctaccaa agtcagtgta tgggtcgtag cacgatcact
    26281 tctttcccag aaatcccaaa ataacaatca atcattatca cagttatctg atgagttcct
    26341 ttaatatcgt tctctatcaa ccggaaattc cacccaatac tggcaatatc attcgacttt
    26401 gcgcaaatac aggatcaagc ctacatcttg tcaagccatt tggttttcaa ttgaatgata
    26461 agcaactgct tcgggccgga ttggactatc acgagtttat caatctgatc acgcataaca
    26521 actgggaaga ttgcatgcgt agctttgcaa acaaaaggat tttcgccgtt tcgacgaagg
    26581 gaaaacaacg cttggatcag gttaactttc ttaaggagga tacctttctt ttcggttcgg
    26641 aaagccgggg gttaccggct gaaatactgg caagcttccc cgatgagcgg aaaattttta
    26701 ttccgatgct accgcatcga cgcagcttaa atctctcgaa tgcagtcgcc attattgttt
    26761 atgaagcttg gcgacaaata ggttatacag cagaagaatc atcagcgatg actaattaat
    26821 gaaacccaaa cttcctgcta acgcattgat aaattgtcat tgagaatacg tggtgtaaca
    26881 aaaataagta attcccgccg atcttcccgt cttaaactat ttttgaataa gttacccaag
    26941 actggaatat cgcccaacaa gggtagttga ttgcgattca gtcgatctgc tgattcaaaa
    27001 atacccccaa taaccactgt tcccccatta tcaacgagca cttctgtagt gatctgtttg
    27061 gtgtcgatcg ctgggcctgc tggtgtatct tgtccacgtg tatcctgatt cacacgcaat
    27121 ctcatgatca cattatcatc cggcgtgatt tgcggggtta ctctaagact aagtacagct
    27181 ttcttaaact gaatttgcgg ccttccgagc tgactcacaa tctggaatgg aatttcggta
    27241 ccttgttcga tgaccgcctc gactcgattt gctgttacca gccgaggact cgcaattacg
    27301 cgtcctcgac tatccgtttc caaagctgat aactccatat taaccagcgt gccattattg
    27361 atttttatca aactcaatcc taatgcagct ggcccacccg ctgctgcagc cagaccactt
    27421 gcaggtaagt tgacatttaa attctgcccc ccccctggac taagcaatcc agatgccaag
    27481 ccactggaat gatccaaatt accggaaatt ccaagattac gattacccat ctgggttgca
    27541 ttttgcacac caaagcgcgc gcccaatgtg cgactgaatg tctccgtcgc ttccacaat
//
LOCUS       DAOQLJ010000012        27108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_588_length_27108_cov_6.193065, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000012 DAOQLJ010000000
VERSION     DAOQLJ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 27108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27108
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_588_length_27108_cov_6.193065"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2885
                     /locus_tag="PK522_01865"
     CDS             <1..2885
                     /locus_tag="PK522_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC40512.1"
                     /translation="DGHMMMRLTFEIGTDIDIAQVQALNRQRLAEPQLPAEVIRQGIT
                     VKKTSPDLLAVVALSSTDPQHDTVYLSNYALLRVLDNIKRLSGVGDAIIFGEQNYSMR
                     IILDPIRMAQLNVTPSEIIAVVQDQNRDFPAGRIGREPTAQGTELTIPVITHGRMSEV
                     HEFEEIIIRAFPDGSMLKLKDVANIELGARSYDLQGRWNGKPNTFLLTFLAPGANALE
                     TVERVRAEMERLAKMFPAGVAYDIPYDTTKFIEVSIEEVVKTLAEATLLVLLVVFLFL
                     QNWRATLIPAVAVPVSLIGTLIGMSALGFSINTLTLFGMVLAIGIVVDDAIVVVENVE
                     RHMNQGGLSSRDAARKAMDEVTGPVIAIVLVLSAVFLPVAFLSGITGELYKQFAITIA
                     LSVILSGFVALTLSPALCALVLKPSHNVSSNLFWKLFNQSFSWLQTRYVGVVSIALKR
                     WVIALAIFLALMVIIVGLFNRIPQSFIPEEDQGYFITVVQLPDGASLTRTTAVLDKVE
                     SFFQSISAVHSTDTLAGQNFVFGTRGTNQATMFVPLNHWDTRKAEQDQIQTLIKRAYQ
                     EFATIPEALILAFNAPAISGLGSTGGFSAQIQDPSGGDLVEFAAIAQEFVTKATEHPA
                     IAIASTNFKISAPQFFIEVDRERAKSLGVSISEIFDTLQAYFGSWYINDFIKYGRVYR
                     VQTEAMPEYRSNPDNLNHIHVRAQSGSSQTMIPLSSVVTTQFNSGPDPVTHFNGFNSA
                     MVLGSAAPGYSSGQALDALQAVADEILLPKGYALDWSGISLQERKAGGQSLLVFAFAL
                     LMVFLVLAALYESWSIPFAVIFAIPLGILGALLAIWLRDLSNDIYFQIGLVTLIGLAA
                     KNAILIVEFANQNYRAGQPLIDATLEAAKLRFRPIIMTSMAFILGVFPLVIASGAGAA
                     SRHSIGTGVFGGMLAATFLAIFFVPIFFVIVNRIAHRVRLRNTRKV"
     gene            2958..3056
                     /gene="ffs"
                     /locus_tag="PK522_01870"
     ncRNA           2958..3056
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK522_01870"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3116..4723
                     /gene="dnaX"
                     /locus_tag="PK522_01875"
     CDS             3116..4723
                     /gene="dnaX"
                     /locus_tag="PK522_01875"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNC40513.1"
                     /translation="MILTQVLARKWRPKVFTELAGQDHIVKAIANSLDRNRLHHAYLF
                     SGTRGVGKTTIARILAKALNCDQGITSQPCGQCTTCLSIDQGTFVDLVELDAASNTQV
                     DHMRELLESAYYAPSVARFKIYLIDEVHMLSKSAFNAMLKTLEEPPNHVKFILATTDP
                     QKLPITVLSRCLQFNLKQIPPALIVSRLQHILSREQIKADLPALQLIARAACGSLRDA
                     LSLLDQAIAYGNGVVEENQVRDMLGTIDLDYLYDLLEALSTQDGRAMIAIADQMEARS
                     LSFDSALQDMGVLLHRLALAQIAPQAIDEAMLDRERFFDLVKRFSPEEIQLYYQIVLH
                     GRHDLSLAPDDYAGFTMTLMRMLAFVPEQDLASFEYNADDKKAEPAILIPNAKPVDEK
                     PVTHDSSQAEQWADSDYTSQQWLSIVKQLKLSGMAKMLAQHCEAKFLSEEKIALYVPE
                     LHKHLLEKRYQDRLKQALEDYFKKTIDIKFSIGTLSGMTVAAIEDQEKQLKQSQAIAA
                     IEADPFVREVVENFDAKLIVSSIKPIN"
     gene            4740..5063
                     /locus_tag="PK522_01880"
     CDS             4740..5063
                     /locus_tag="PK522_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNC40514.1"
                     /translation="MKANLGNIMKQAQQMQENMKAMQEQLASIEIEGQSGAGMVKVVM
                     TCRHDVKRVSIDPSLLGDDKEMLEDLIAAAVNDAVRRVETTTQEKMSGLTGGLGLPPG
                     FKLPF"
     gene            5132..6028
                     /locus_tag="PK522_01885"
     CDS             5132..6028
                     /locus_tag="PK522_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNC40515.1"
                     /translation="MRTSRPLRSPRKPSQPTENRSQPKRVDQAVTHKLQKLLAQSGLG
                     SRREMEALIAEGQVSINGKVAKVGDRISTGDLIRVKNRLIRINTGSRLPRIMFYHKPE
                     GEIVSCHDPEGRPSVFDKLPQLKSSRWVSIGRLDFNTSGLLIFTTSGELANQMTHPRF
                     QIEREYAVRIIGQLTPEQMTQLTRGVELEDGIAKFDMIVEQGGEGSNRWYHAVIKEGR
                     NREIRRMFEKLGIAVSRLIRVRFGPIRLPPYLKRGMLSELKENEVRQIMALFNKMIEP
                     KIVVPRVQSNQSKFTSNRFSST"
     gene            complement(6000..6923)
                     /gene="prmB"
                     /locus_tag="PK522_01890"
     CDS             complement(6000..6923)
                     /gene="prmB"
                     /locus_tag="PK522_01890"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNC40516.1"
                     /translation="MTHSNNTAPTLIQQAKTELRTVRDWLRLTTSYFSQAQLFFGHGF
                     PTAYDEAAHLILHVLHLAPNQLDLFLDAQIIESERNAMIELIEKRVSERIPITYLTHE
                     AWLGDFNFYVDQRVIIPRSFIAELLQTQLSPWISNPISVTRALDLCTGSGCLAILLAH
                     AFENAQIDAVDISPSALEVAHINIENYDLASSIHLIHSDLFSKLGNKRYDLIISNPPY
                     VNAESMDDLPEEFRHEPSIALAGGFDGLAIVRNILSNAADFLNDEGLLIVEIGHNRSA
                     LEQLYPRMPFTWLETSAGDQFVFMLKKNDLK"
     gene            7027..7509
                     /gene="tadA"
                     /locus_tag="PK522_01895"
     CDS             7027..7509
                     /gene="tadA"
                     /locus_tag="PK522_01895"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNC40517.1"
                     /translation="MMDEEWMRLALGLAEKAQELDEVPVGAIIVKNGEIIGQGYNQPI
                     KTNDPSGHAEMIAIRAAGQQIQNYRLEECTLYVTLEPCAMCIGAIFHARLRRLVFAAS
                     DPKTGVCGSILDLPAEKRLNHHLEVHKGLLAEESARLLRQFFYGKRHLDKLARYENSN
                     "
     gene            7493..7972
                     /locus_tag="PK522_01900"
     CDS             7493..7972
                     /locus_tag="PK522_01900"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNC40518.1"
                     /translation="MKIQINIEQQQLDLFDREERLVKRYLISSAKNGIGQVRGSYCTP
                     LGKHIIRAKIGDKQPVNTVFVKRRPTGEIYTPQLAAQYPGRDWILTRIFWLSGCEVGF
                     NRLGIVDTMRRYIYIHGSPDSAVMGKPGSIGCIRMHNDDLLELFDKVPVGTSVNITQ"
     gene            complement(8068..9336)
                     /locus_tag="PK522_01905"
     CDS             complement(8068..9336)
                     /locus_tag="PK522_01905"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC40519.1"
                     /translation="MSLVATVIPIEKASSKILVSMFMIYTAFICVSQSWANDRQETAK
                     QERVLLKADRIFDGRTLHEDHALLIDNGKVIEIGPAPQIRKRGGKELDLGDATILPGF
                     IDLHAHLALQNVSHHAVLRHGVTTVRDVGGPLRAPAGGDGSLRVLTTGPIITAPGGYP
                     IPVFGQHGHGGGDHGHSDVALVVETPDQAREAVRHLIQGGAVNIKIALEPGGEPGAPW
                     TTGHAPSVMPPWPLLPLEIIQAIVDETHTLGKKVTAHVGEQQGVELALAAGVDEWAHV
                     PCMEISGDLLQQAVQQGVRIVTTIDTLSLCPGVYSNTLRLAQLGAQFYYGAEIAHIEI
                     PWGIDARELQLMLHLTGMTPLALFETATAKAGEALGLSPLGTLIVGAPADIIAVKGNA
                     FENVKLLEYPDLVISGGRLIVNHFPSKKAK"
     gene            9669..10607
                     /locus_tag="PK522_01910"
     CDS             9669..10607
                     /locus_tag="PK522_01910"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNC40520.1"
                     /translation="MKQVKLISVLLSTLIFLIISSAIVMAKSSELEFRGAVTNIAIPD
                     GDGDSIVKLKLITSHSPDFEVPIIVLGATEIESNGYHIPLSELEVGDVVKINGFFGED
                     GIVAESIYVLDGGLGKFRLRGLIKGVEPVEDGIFMQVLGIDILVDESTSIRSRGLGVV
                     NNLPISALQVGESVDVKGRYLDDTFFADRVEVGKRPDEHVRVKGKIIDLTNDIIEIVT
                     IHEAHLIVVRTGTTKIYGSLAEGEFVEVEGYLNQKLDVIAEKIIVDDDRNGYSRDYHH
                     YESKRSDDDRFGVINRNYGKHHGKKNGRDKDEDRDD"
     gene            complement(10659..12020)
                     /locus_tag="PK522_01915"
     CDS             complement(10659..12020)
                     /locus_tag="PK522_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40521.1"
                     /translation="MKDISTIILLIFTYGLMLNTVAGTFLFAEDNETADVITHPQGYQ
                     GQGTNLIVTVGISPSSLHGTEIALPIQNAINTWNQLIPTLGNIVSDERIPFNHFDFES
                     VVLHELGHCLGLAHPNLSSESGLSGENRNYTQTTRGSNNRYDLNSGSDGIAGSNDDLR
                     GDDANLHWFRRKNNNPFTIAEKIDITTYSRNLSDLPVGHTFAANGDRTVANLLGYQNT
                     EAVMQQGIIAGETQRTLTADDVATLKLAMSGLDRFAGTPDDYTLALTFIGFTDDADIV
                     VSFNNSRSSFAVCETRAEQLNSKHFAITRSRIYFNTNVQWYFNDLTSPNPPKLSYPSP
                     TIAANNQTGTLQLSRNDTLLLTVSLDPGVLLENPAEYWIRAITPTETFWLNDRFEFIP
                     SSQPIRAHVGEFNAINQFIIMKSSLKALQPGNYSITFAVDDNQDGIANGTFHNTVNIT
                     ITP"
     gene            complement(12052..12684)
                     /locus_tag="PK522_01920"
     CDS             complement(12052..12684)
                     /locus_tag="PK522_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40522.1"
                     /translation="MTRLFRSFPLVLKISSTLQMLLGLVLLIIGLSISHAENLLKSET
                     IIAHFEEKQSSTRNFNENITVTIFADGYVKVYRPSYYKKSGIFSGHLDPVALNQLWNQ
                     LTSPSLLNFNETEIQHRLKTAKNQQEKNTLLLQRVSDAPTIIIEIYPNRYMPTASIDQ
                     NNWNTTKRITWYALQWTVENFPELEELHELMEVRELFENILESSELKSLN"
     gene            12869..13561
                     /locus_tag="PK522_01925"
     CDS             12869..13561
                     /locus_tag="PK522_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC40523.1"
                     /translation="MILLASSYEEMLDEWRRSLTDVSSVLCINDHELLNHELARRKPP
                     VLLIDHDFLGNDGSKKIEGLLALSPKTQIIISSPGISDEMEWKLFKSGIRGCYRDSIE
                     PGEIKRVVKAVWQGELWIRRKLVHHMLDELVVTTKEKKHIERAVNNLLENLTKREYEI
                     AILVGRGESNKQIARRLDITERTVKAHLTEIFRKLAITDRIKLALLVKDTAVSSNQTV
                     LHHSNNFPIASA"
     gene            complement(13619..14908)
                     /locus_tag="PK522_01930"
     CDS             complement(13619..14908)
                     /locus_tag="PK522_01930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC40524.1"
                     /translation="MTTSQSQISNLLQKAAHCHQQGNFQDAEACCLKVCALVPNQPDT
                     LHLLAIIYAQTKQYELANNYFIKAITVAPQRADFLGNYANALWEQGNIDDALINCEKS
                     LALNSNQADVHNILGNVYLAQNRIEAAIGCFRNALQYRPNFPQALNNLGNALQKINKM
                     EEAVKSYSNALGLNENYPEAYNNLGQALKTLGRIEEARNYFLKAIALRPNFTQAILNH
                     QEVAMIWLAPLEGNKLYLRRYCKEDASYLRQCYQNTQFMGQYNHYIPRHQRAEELALK
                     LDEANKMHPCQLKSIDWVIMKKGANQPIGIANLVGIEFAHRRAEFLIGLPDTNNHAKG
                     LGLEATLLVLDFAFNKVNLNKITSIVYEDNTVSQQNALALGFIQESMLRDHIFDSTSG
                     KFLNLWGIGMTMNDFRNNLRIAKLSDRLLGKNITLVP"
     gene            complement(14986..17820)
                     /locus_tag="PK522_01935"
     CDS             complement(14986..17820)
                     /locus_tag="PK522_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNC40525.1"
                     /translation="MATNTKTNVLSTASFTKNSKANNDSFENLSSNNTTYVLDVLAND
                     SGGQSQTLWSIDDSASSSAPPSPDLLSPDEIGAINLSEKGANIWITEEGKVAYELTPD
                     LASALKALPQGENLVDTFSYAMRQGKGNNPLSWATATVTFAGVNDAPELTGAPTILSD
                     GIVNQPYSILATDLLSGYTDADGDPLSISNLTATHGLLTTIPDGWLFTPDSDYSGPVE
                     LNYDVIDNQGGSLPAQQNFTVITPGDNTAPSFLSSRPTDDQTTFGNDENIFLYFDEAI
                     KAGTGVITISNGLDTRVIDATDTSQISFTSSKGSHYVTINPHDDLIPNTTYNVQMGAG
                     AITDLVGNPFAGIADENTLNFTTVLSLPQLIGSNPWDDATQFKIDSNIELYFDETVMA
                     GSGDIIISNGVDTRIIPINDTNQVSFSKDGSTITINPTADLIPNTDYNVQIASGVITN
                     LAGNPYLGINDSRTLNFTTIPSNPLLIGSYPWDDSSEFQVDSDIVLSFDEGVKTGIGN
                     ITITNEADSTDSRVISVNDATQVLFDDSGYVIINPMDNLASNAEYSIQIDSGAIIDLA
                     GNAYAGISDNTSLNFTTIPTNPLLNWSNPPDDFNHFQVENNIELFFNESIIAGSGNII
                     ISNGVDTRIIPITDTSQVSFDQYYSAIVTINPTDDLIPNTTYHIEIESGVINDLAGDP
                     FLGISDDTTLNFTTTLNPFLIGSSPWDDSTDFKIDDNLWLSFDETVKAGVGNITITNE
                     TNPADSRMISVDDITQVSFDDFGNVIINPTADLIPNTLYSIQIDNTAITDLAGHAYIG
                     ISDNTTLNFTTIPSNPRLSFSTPADDTILFPTDGNITLSFDEQITAGIGNIIITNESD
                     TSDTHTFAINDTSQVTISGSKVTIDPSTDLMPNTTYNIQIAEGVIKDLAGNTYKGIQD
                     ADTLDFTTGDAFQTSTVGINEFNLPILPLG"
     gene            complement(18149..19114)
                     /locus_tag="PK522_01940"
     CDS             complement(18149..19114)
                     /locus_tag="PK522_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40526.1"
                     /translation="MDRLTIIILMMLVSNIAIANYFNTHRSQKIIGSFVTEKSQIQEV
                     NSFSNAFAFAPASPRDITQYLDENKGRMLPVISISRLLLDEQTGMYHHDVTSIVEAIA
                     KAKHKDTEILFLMDEPFWWVRRACEEKGKAEACLDVANRYVETLSTFRIAGQLLRKHL
                     PGSGIIHIEAWAELVLQKKAYPEENVVMLDDAEYLGFDCYGGIEFCGSDEYGYYSQFE
                     YGTWVWDAMLAMESQQAIGRKLFVIPGTFLADGHFADIDTLINQIGFYGWILNQSDKI
                     GGFGAFLWGDMVENNKPFTGGRNILQATNFLKVIARYYGVKTNEK"
     gene            complement(19304..20203)
                     /locus_tag="PK522_01945"
     CDS             complement(19304..20203)
                     /locus_tag="PK522_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNC40527.1"
                     /translation="MRIGIDLGGTKIEGVVLDRNSTELLRKRIETRQVEGYQAILHKI
                     AELVLLLENEVGKPCSVGIGTPGAISAVTGKMKNSNTACLNGQPLLEDLQALLNRPLR
                     IANDANCFTLSEALNGAGTGYNVVFGVIMGTGVGGGIVFKGQLHSGHQHIGGEWGHNI
                     LESNGPDCYCGQKGCVETFLSGPGLAADFYRNGGNTEITTNGIVALAAQGDSLAEAAM
                     QRFFDRFGRAMATVVNILDPDVIVLGGGLSNIDRLYTEGRAKVAQYVFNDQFITPMLR
                     NIHGDSSGVLGAAQLWRHDDAFE"
     gene            20312..21583
                     /locus_tag="PK522_01950"
     CDS             20312..21583
                     /locus_tag="PK522_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC40528.1"
                     /translation="MKFETIAIHGGYSPDPTTKAVAVPIYQTTSYAFDDTQHGADLFD
                     LKVTGNIYTRIMNPTTAVLEQRMAELEGGIGALGLASGQAAITYSILTIAEAGDNIVS
                     VGTLYGGTYNLFAHTLPQYGLTVRFADYRNPENFKAMIDEKTKALYCESIGNPLGNVV
                     DIAALAEIAHERGVPLIVDNTVPTPYLCRPFEYGADIVVHSLTKYLGGHGNSIGGVLI
                     DSGKFPWADHPNRFKRLNTPDVSYHGVVYTEALGPAAYIGRARVVPLRNMGAAISPFN
                     SFLILQGIETLPVRMDRICSNTLVVAQYLKTHPKVSWIKYAGLPEHPEHPLTQKYMSG
                     KASGILTFGVQGGIAGGARFQDALKLITRLVNIGDAKSLACHPASTTHRQLSAEEMQK
                     AGVSEDMVRLSIGLEHIDDLIDDLEQALAAV"
     gene            21811..22362
                     /locus_tag="PK522_01955"
     CDS             21811..22362
                     /locus_tag="PK522_01955"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HNC40529.1"
                     /translation="MKKYIHFPSTFKKAMLGLSILIGSLSNLGHAQDQLGLLPLSSLR
                     VSDAQAYETCPAVNPSIRHADFVVILQRQANGPINTETVEFSTHDGTALSGVNYVPVS
                     GKLTFLPGETVKSVRVIILSSQNTDDSYFFLTLSNPSIQTVLRDFNGTASIAHLDPEN
                     ETCRNRVSLNADPRLPDPPPVPK"
     gene            complement(22422..23138)
                     /locus_tag="PK522_01960"
     CDS             complement(22422..23138)
                     /locus_tag="PK522_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNC40530.1"
                     /translation="MRYAVLIIGIYHYKNVPPLRATSDAEDIANLFAEMSLAYPFSSQ
                     TLLLDTQATELAIINALDALAGETDENTLVFIYFAGHGVRAAQPGRFWYYLLPFDGKA
                     DDLTQLEGSAISMEKFSNKLGAINALQWVLVLDCCKAGSIAEHLSRSLPKENNRNWAI
                     LAATTGDSNSYALPHRRHSCFTQYLLEGLSGRAIDQSGTVRIMNLIDYIQRNIQQEPI
                     LQQPVLKARLHQNFVLKHCM"
     gene            complement(23161..23553)
                     /locus_tag="PK522_01965"
     CDS             complement(23161..23553)
                     /locus_tag="PK522_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40531.1"
                     /translation="MRTALYVLKPTTVSIQPIDPLDHHAMLFAYDSNVPEQSATGIIN
                     LTTGIYLITSRNQLDISGDNIHTENFEKDLGNEQLHQICGGMTEPHISIRGKSEVPDI
                     STHYEKLEITTTHESIKQFFQIERGIEI"
     gene            complement(23654..23872)
                     /locus_tag="PK522_01970"
     CDS             complement(23654..23872)
                     /locus_tag="PK522_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40532.1"
                     /translation="MNMSLAIPKTAIISIIFVITSLTSLSARAEFVTPRGLGSPEQVS
                     IVFQPSIQHLTQSGFIYEDDPDEGGQVE"
     gene            complement(24063..24332)
                     /locus_tag="PK522_01975"
     CDS             complement(24063..24332)
                     /locus_tag="PK522_01975"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNC40533.1"
                     /translation="MKKLFILLLTFSLFACQGIPENVTVVNGVNANQYLGTWYEIARL
                     DHSFERGLEQVTANYSKNADGSIKVINRGFNTEKQAWREAEGKVR"
     gene            complement(24344..24796)
                     /locus_tag="PK522_01980"
     CDS             complement(24344..24796)
                     /locus_tag="PK522_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HNC40534.1"
                     /translation="MKVWVDADACPVVIKEILFKAANRTNIMTTLVANKLIYVPPSPN
                     IRTMQVENGFDVADNKIVELLEAGDLVITADIPLAAQVIERGGYALNPRGEFYAKENI
                     QQHLSMRNLLEELRASGIETKGPSTFNQSNRQAFAAQLDRFLTGVIKR"
     gene            complement(24938..26383)
                     /locus_tag="PK522_01985"
     CDS             complement(24938..26383)
                     /locus_tag="PK522_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC40535.1"
                     /translation="MRAVSDAGYTTPTPIQAKAIPQVLSGGDLLAGAQTGTGKTAGFT
                     LPLLHILSNTPVKYPAEKHRPRCLILTPTRELAAQVEESVKIYGKYLKLTSTVIFGGV
                     NINPQTSRLRKPLDILVATPGRLLDHANQHNVDLSGIEILVLDEADRMLDMGFIRDIK
                     KLLAMLPKQRQNLLFSATFSDEIKQLADGLLHNPAFVEVSRRNTATELVEQTVHLVEQ
                     ALKRDLVSFLIKQNNWQQVLIFTRTKHGANRLADKLIKDGIAAMAIHGNKSQGARTKA
                     LAEFKSGQIPVLVATDIAARGLDIDQLPQVVNFELPNVPEDYVHRIGRTGRAGTTGAA
                     ISLVDVEEIKFLKNIEKLIKREIPKVAVEGFIPPAKLETESPRLFREQQPNRSRMPKQ
                     GQPNQGQSKQKQNKQNQPKRSQTQGRDRNSHANDSSNSKPAIGYGQGKLISPAPQKGK
                     LLSEAARHQAQVQMPLFNPKPPRYRGGGRGH"
     gene            complement(26595..26809)
                     /locus_tag="PK522_01990"
                     /pseudo
     CDS             complement(26595..26809)
                     /locus_tag="PK522_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611295.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         7582 a         5465 c         5960 g         8101 t
ORIGIN      
        1 atgatgggca catgatgatg cgcctcacat tcgaaattgg aaccgatatc gatatcgctc
       61 aagtacaagc cttgaatcga caacgactag ctgagccgca attaccagcc gaagtaattc
      121 gacagggcat tactgtcaaa aaaacttcac cagatctttt agccgttgtt gcgttaagtt
      181 ctacggatcc tcaacacgat actgtttatc tatcaaatta tgcgctactc agagttcttg
      241 ataatattaa gcgattatct ggtgtaggtg acgcaattat atttggtgag caaaattatt
      301 cgatgcgaat tatccttgat cctattcgga tggcacagct caatgtaacg ccgagcgaaa
      361 ttatcgcggt ggttcaggac cagaatcgcg attttccagc gggacggatt ggtcgtgagc
      421 ctactgcaca aggaacggaa cttacgattc ctgtcattac gcatggcaga atgagtgaag
      481 tccatgagtt tgaagaaatc attatccgtg cttttcctga tggatcgatg ttgaaattaa
      541 aagatgtggc caatatagaa ttaggtgccc gttcttatga tcttcaaggc cgctggaatg
      601 gtaaacccaa taccttcctt ctaaccttct tagccccggg tgctaatgca cttgaaacag
      661 tggaacgagt acgtgccgag atggagcggc tcgctaaaat gtttccagct ggggtagcgt
      721 atgacattcc gtacgataca accaaattta ttgaagtatc gatcgaggaa gtggttaaaa
      781 ctttagctga agcgacatta ttggtgctac tcgtagtttt cctctttttg cagaattggc
      841 gtgctacgct gattcctgca gtggctgttc cggtatcatt gattggtaca ctgattggta
      901 tgtcggcact gggtttttca atcaatactc ttacgctatt tgggatggtt cttgcaatcg
      961 gaatcgtggt cgacgatgcg attgttgtcg tggagaatgt agagcgtcac atgaatcaag
     1021 gaggactttc gtctcgagat gcggcccgga aagcgatgga tgaagtaacg ggtccggtga
     1081 ttgcgattgt tttggtattg tcggccgttt ttttaccggt ggctttttta agtggaatta
     1141 ccggtgaatt gtataagcag tttgccatca cgatcgcgct atcagtcatt ctttctggtt
     1201 ttgttgcttt gacgttgagc ccagcgcttt gtgcgttggt actgaaaccg agtcataatg
     1261 tttcttccaa tctgttttgg aagctgttta atcagtcatt tagttggtta caaactcgtt
     1321 atgttggagt tgtatcaatt gcattgaaac gatgggtgat tgcactcgct atttttcttg
     1381 ctttgatggt cataatagtt ggtttgttta atagaattcc tcaaagtttt attcctgagg
     1441 aagatcaagg ctattttatc actgtggtgc aattgcccga tggcgcttcg ctaactcgaa
     1501 caacagcagt gctcgataag gttgaaagtt tttttcaatc aatttctgcg gttcattcaa
     1561 cggatacatt agcgggccag aattttgtgt ttggcacacg gggcactaat caggcaacca
     1621 tgtttgttcc cctgaatcac tgggatacac gaaaagcaga gcaggaccaa attcaaacct
     1681 tgattaagcg cgcttatcaa gaatttgcaa cgattccaga ggcattgatt ctagcgttta
     1741 atgcgcctgc aattagtgga ttaggctcta caggaggatt ctccgcacaa atccaagatc
     1801 cgagtggggg cgatcttgtc gagtttgctg caatagcgca agaatttgta actaaagcaa
     1861 ctgagcatcc cgcaattgca atagcgagta ccaatttcaa aatcagcgca ccgcagtttt
     1921 ttattgaggt tgatcgggaa cgtgcaaaat cgttaggcgt ttctatttca gaaatttttg
     1981 atacgctgca ggcttatttt ggcagttggt atatcaatga ttttattaag tacggccgtg
     2041 tttatcgtgt gcaaacagaa gcgatgccgg aataccgttc gaatcccgat aatcttaatc
     2101 atattcatgt gcgtgcccaa tccggttcat cacaaacgat gatccccttg agttctgtcg
     2161 taacaactca atttaacagt gggcctgatc ctgttaccca ttttaacgga tttaattccg
     2221 caatggtgct tggaagcgcg gcaccaggct atagttcagg gcaagcgttg gatgccttac
     2281 aagccgtagc ggatgaaatt ctactcccca aagggtatgc cctcgattgg agtggcatat
     2341 cgttgcaaga acgtaaagca ggcgggcaat cgctattagt atttgctttt gcgctattga
     2401 tggttttctt agtattagcc gcattgtatg aaagttggtc gattccattt gccgtgattt
     2461 ttgctattcc actaggaatt ttaggggcat tattggcaat ttggttgagg gatttaagta
     2521 acgatattta ttttcagatt ggattagtca ccttgattgg attagcagca aaaaatgcaa
     2581 tattaatcgt tgaatttgcc aatcagaatt atcgagcggg acaaccactg attgatgcaa
     2641 cactggaagc agcaaaacta cgtttccgac ccattatcat gacatcaatg gcttttattc
     2701 taggtgtttt tccattggta attgcatcag gggctggtgc tgcaagccgt cactcgatcg
     2761 gaacgggtgt ttttggagga atgttggcag ctactttttt ggccatcttt tttgtaccca
     2821 tttttttcgt catcgtaaat agaattgcac accgagtgcg tttgaggaat acgcgaaagg
     2881 tttgaatggt gattcgaaaa aatggatatg ctttaatttc ggattgtgat ctgaatcatg
     2941 tacaatagca aacctaaggc gggcctcccc gcattgtacg gtagtgaacc tggtcaggtc
     3001 cggaaggaag cagccacaac tattttatac aagtgccggg ggtcaggctc gccaccttcc
     3061 aaactttgat acgcagcgtt tccttttcgt ttatcaacct tcctcaaagt atactgtgat
     3121 cttaacccaa gtgcttgcca gaaagtggcg acctaaagtt tttactgaac tggcagggca
     3181 ggatcatatc gtcaaagcaa ttgcaaattc gcttgatcgg aatcgtttgc atcatgccta
     3241 tttgttttcg ggaacgcgtg gtgttggtaa aacgacaatc gcccgaattt tagcgaaggc
     3301 cctcaattgt gaccaaggca ttacctctca accctgtggg caatgcacga cttgcctcag
     3361 tattgatcaa ggaacctttg tggatttggt tgaattggat gctgcatcaa atacgcaagt
     3421 agatcatatg cgcgagctac ttgaaagcgc ttattatgcg ccatccgtgg cgcgttttaa
     3481 gatttatctg atcgatgaag tccatatgct ttctaagtcc gcatttaatg caatgctgaa
     3541 aacacttgaa gaacctccca atcatgttaa gtttattcta gcgacgacag atccccagaa
     3601 attgcccatt accgtacttt cgcggtgcct gcaatttaat ttgaaacaaa ttccacctgc
     3661 gctgatcgtg agccgattac agcatatctt atcacgagag caaataaaag ctgatttacc
     3721 agcgttacag ctaatcgcga gagcggcctg tgggagtcta cgcgatgcac ttagcttact
     3781 cgatcaggca atcgcctatg gaaatggtgt ggtcgaagaa aatcaggttc gagatatgtt
     3841 aggaacgatt gatctggatt atctctatga tttgcttgaa gcattgtcaa ctcaagatgg
     3901 gcgtgcaatg atagcgattg cagatcagat ggaggcccgt agcttatcgt ttgatagcgc
     3961 gttacaagac atgggggtat tattgcatcg tctcgcttta gcgcagattg cgcctcaagc
     4021 gattgatgaa gccatgctag atcgcgagcg tttctttgac ttggtaaaac ggttctcgcc
     4081 tgaggagatc caactgtatt accagattgt gcttcacggt cgtcatgatt taagcttagc
     4141 accagatgat tatgccggtt tcacaatgac gcttatgcgc atgctcgctt ttgttccaga
     4201 gcaagattta gctagctttg aatacaatgc agatgataaa aaagctgagc cagcgatact
     4261 catacctaat gctaaaccag ttgatgaaaa gcctgtcact catgattcca gtcaagcaga
     4321 gcagtgggct gatagcgatt atactagcca acagtggttg tccattgtta agcagctaaa
     4381 gttgagtggt atggcgaaaa tgcttgcaca gcattgtgaa gcaaaatttt tgtcagaaga
     4441 aaaaatagcc ttatacgttc ctgaacttca taaacatttg ttggagaagc gttatcaaga
     4501 caggcttaaa caggccttgg aagattattt taaaaaaaca atagatataa aattttcgat
     4561 tggtaccctt tcaggaatga ccgttgcagc gattgaggac caagaaaaac aacttaagca
     4621 atcgcaggcc attgctgcga tcgaggcgga tccctttgtc agagaggtgg ttgaaaattt
     4681 cgatgcaaaa ttaattgttt cttcaattaa acctataaat taaaggagag aatcaaatga
     4741 tgaaagcaaa tttaggcaat atcatgaagc aagcacagca aatgcaggaa aatatgaaag
     4801 cgatgcagga gcaacttgct agtatagaaa tagagggtca atctggggca ggtatggtta
     4861 aagttgtgat gacttgtcgc cacgatgtca agcgagtaag tatcgatccg agcttgttgg
     4921 gagatgacaa agaaatgctg gaagacttaa ttgcagccgc tgtgaatgat gctgttcgtc
     4981 gagtcgaaac gacaactcag gaaaaaatga gtggcttgac gggcggtcta ggattaccgc
     5041 caggatttaa attacccttt tgattttcta tcagaacgga cggtatgttg tatcgtgaat
     5101 cataatgaat tgctctgctt gaaggtgttt tatgcgaaca agtcgccccc ttagatcacc
     5161 tagaaaaccg tctcaaccaa cggaaaatcg atcgcaaccg aaaagagttg atcaggccgt
     5221 tacgcataag ctgcaaaagc tattagcaca aagtggttta ggttcgaggc gggaaatgga
     5281 ggcattgatt gctgagggac aagttagtat caatgggaaa gttgctaaag ttggggatcg
     5341 aattagtacg ggtgacttaa ttcgggttaa aaaccgactg atccgaataa ataccggtag
     5401 tcgtcttcca agaatcatgt tctatcataa accagaaggt gagattgtta gttgccatga
     5461 tccagaggga agaccttcag tctttgataa gctgccacaa ttaaaatctt cgcgttgggt
     5521 ttcgattgga agattagatt ttaataccag tggtttattg attttcacga caagcggtga
     5581 attagcaaat caaatgacgc acccacgctt ccagattgag cgcgaatatg cagtacggat
     5641 cattggtcaa ttaacgccgg aacaaatgac tcaattgact cgtggcgttg agcttgaaga
     5701 tgggatagcc aagttcgata tgattgtcga gcaagggggt gaaggttcca accgatggta
     5761 ccatgcggtc atcaaagaag gccggaatcg agaaattaga aggatgtttg agaaactggg
     5821 aattgcggtg agtcgcctga ttcgtgttcg gtttggccct attcgtttac caccttactt
     5881 gaaacggggc atgttgtccg aactcaaaga aaatgaagtt cgccaaataa tggctttatt
     5941 taataaaatg attgaaccaa agatcgttgt tcctcgggtt cagtccaatc aatcgaaatt
     6001 tacttcaaat cgtttttctt caacataaat acgaactgat caccagcact tgtttcaagc
     6061 caagtaaacg gcattctagg atatagctgc tccaaggcag accgattatg gcctatttcc
     6121 acaatgagta ggccttcatc atttagaaaa tctgctgcat ttgacaaaat gttacgaaca
     6181 atggcaaggc catcaaaacc tccggctaga gcaatggagg gttcatggcg aaactcttcg
     6241 ggaagatcat ccattgattc cgcattaacg tagggtggat tgctgataat gagatcatag
     6301 cgcttgttac ccagcttaga gaaaagatcc gagtgaatca agtgaatgga agaagctaaa
     6361 tcatagttct caatattaat atgggctact tctagcgcgc tgggagaaat atcaaccgca
     6421 tcaatttggg cattttcgaa agcgtgcgcc agtaagatcg cgaggcaacc cgatcccgtg
     6481 cagagatcta atgcgcgagt caccgaaata gggttgctga tccaaggtga tagctgggtt
     6541 tgtaataatt cagcaataaa cgatctcggt ataatgacgc gttgatcgac gtaaaaatta
     6601 aaatctccta accatgcttc gtgggtgaga taagtaatag gtattctttc agaaactcgt
     6661 ttttcaatta attcgatcat tgcattacgc tcggactcaa tgatttgagc atctagaaat
     6721 aaatcgagtt gattcggcgc aagatgtaac acgtgtaaaa taaggtgtgc tgcttcatca
     6781 taagcggttg gaaagccgtg tccaaagaat agttgtgctt gactaaagta gctagtagtg
     6841 aggcgtagcc agtcgcgtac agttcgtaat tctgttttgg cttgttgaat tagggtgggg
     6901 gcagtattgt ttgagtgtgt cacgatcaat ctagatagaa gtttgtgata aaaaattgta
     6961 taagtttaga atgatagcgt ggaatggttg gatatcgaat aaattgtgat tttagatagc
     7021 ctgggtatga tggacgaaga atggatgcgg ttggcgcttg gattagcgga aaaagcgcaa
     7081 gaattggatg aagtccctgt tggtgcaatc atcgtcaaaa acggtgaaat tattggtcaa
     7141 ggttataacc agccgatcaa aacaaatgat ccctcgggcc atgctgaaat gattgcgatt
     7201 cgcgctgccg gtcaacagat tcaaaattac cggttagaag aatgcacgct ttatgtcacg
     7261 ctggagcctt gcgcgatgtg tattggtgca atttttcacg cccgattgcg ccgccttgtg
     7321 tttgctgcaa gtgatcctaa aacgggtgta tgtggaagta ttttggattt gcctgcggaa
     7381 aaaaggctta atcatcatct ggaagtgcat aaaggtctgc ttgctgaaga gtcagctcga
     7441 ttgttgaggc agttttttta tgggaagcgt catttagata aactagctcg gtatgaaaat
     7501 tcaaattaat attgaacaac agcagcttga tttgtttgat cgtgaagaaa gattggttaa
     7561 acgctattta atttcttcgg ctaagaatgg catagggcaa gtaagaggga gttattgtac
     7621 gcccttgggt aagcatatca ttcgtgcaaa aattggcgat aagcaaccgg ttaacacggt
     7681 tttcgtcaaa cggcgaccca caggtgagat ttatacgccc caattagcgg cccaatatcc
     7741 tggccgagat tggattctga cacgaatctt ttggttatcg ggttgtgaag tcgggtttaa
     7801 ccggctagga atagtcgata ccatgcgtcg gtatatttat attcacggga gcccggatag
     7861 tgcggtaatg ggtaaacccg gttcgattgg ctgtattcgt atgcataacg atgatctatt
     7921 agagcttttc gataaagtgc cggttgggac atctgtcaat atcacgcaat gatttttaca
     7981 ttgctgagcc taaatagcta aatcattgca gtcttatgag aattcactcg ttattgcaac
     8041 gatttagctg attgtttgat cgtccgatta tttggctttc ttagaaggaa aatgattaac
     8101 aattaatcga ccacctgata ttactaaatc agggtactcc aacagtttaa cattttcaaa
     8161 ggcatttcct tttactgcaa tgatgtcggc aggcgcacca acaatgagcg tgcccagtgg
     8221 tgatagaccc aatgcttcac ctgctttagc agttgcggtc tcaaataacg ctaatggcgt
     8281 cattcctgtc agatgaagca tcaattgtaa ttctcttgca tcaatacccc atggtatttc
     8341 gatatgggct atttctgcgc cataataaaa ttgtgcgcca agttgagcca gccttagcgt
     8401 atttgaataa acgccaggac ataaagacaa ggtgtcaatg gtcgtcacaa ttcttacccc
     8461 ttgttgtact gcttgctgaa gtaaatcccc cgagatttcc atacagggaa catgcgccca
     8521 ttcatctact ccagcagcga gcgctaactc aacgccctgt tgctcaccta cgtgtgcagt
     8581 tacttttttt ccaagtgtgt gcgtctcatc gacaatcgct tggattattt caaggggtaa
     8641 taagggccag ggtggcatca cggatggcgc gtgcccagtt gtccatggtg cgcccggctc
     8701 tcctcccggt tcaagcgcta tttttatatt aactgctcca ccttgaataa gatgtcgaac
     8761 ggcttccctt gcttgatctg gggtctcaac tactaaagca acatcgctat ggccgtgatc
     8821 accgccgcca tggccatgct ggccaaagac agggattgga tagccgccag gtgctgtgat
     8881 tatcggtccg gttgtcagaa cacgcaggct gccatcgcct cctgctggtg cccgcaatgg
     8941 cccacctaca tcccgaacag ttgtcacacc atgacgaaga actgcatgat gagaaacatt
     9001 ttgcagcgct agatgagcat gtagatcgat aaaaccaggc agaatagttg catctcctaa
     9061 atctagttcc tttccaccac gcttgcgaat ttgtggggct ggtccgattt cgatcacctt
     9121 gccattatcg attaataacg catgatcttc atgcagtgtg cgtccatcaa aaatacgatc
     9181 tgcttttaat aagacgcgtt cttgctttgc tgtttcctgg cggtcatttg cccaactttg
     9241 cgatacacag atgaatgctg tgtatatcat gaacatactt actaagattt tacttgatgc
     9301 tttctcgatt ggaattacag ttgcaaccaa actcatcatt ccttcctttt tgttgtagtt
     9361 atttgaattt attttcttaa ataatgcagc gacgtgtggc cattatagga ataaaaatta
     9421 atgatggaat gtgacaaatt gtcgcagcac aattgactgc gattattatc tgaatactgc
     9481 cagcattaaa tctggccgcg tcgaattttt ttacagccta agcattaata gatagacaaa
     9541 tctagattaa tcattaattt gtagaaatgg catagatatt gcttttttta cttggtacaa
     9601 gtatccacaa ttttcggttg agatttttta taaacaaaaa agtattttgg agttctctag
     9661 aggaaataat gaaacaagta aaattgatct cagtattatt atcaacgctg atatttttga
     9721 taatctcttc tgccatcgtg atggcaaaga gttcagagct tgagtttaga ggtgctgtaa
     9781 cgaatatcgc tattcctgat ggcgacggtg atagcattgt taaactgaaa ttgattacca
     9841 gccactcccc tgattttgaa gtgcctatca tcgtgttagg tgccacagag attgagtcga
     9901 atggttacca tatacccttg tctgaattag aagttggtga tgtcgtaaaa attaacggtt
     9961 tctttggtga agatggcatt gttgcagaat caatttacgt gctagatggt ggattaggta
    10021 agtttcgctt acgcggttta attaaggggg ttgagcctgt cgaagatggc atttttatgc
    10081 aggtgttagg tattgatatt ctagtcgatg aatccacctc aattcggagt cgaggactgg
    10141 gcgtggttaa caatctgcct atatctgcgc ttcaagtcgg tgaaagtgtc gatgtgaaag
    10201 gtagatatct cgatgacaca ttttttgcgg atcgcgttga agttggtaag cgtccagatg
    10261 agcatgtgcg cgtaaaagga aaaattattg atcttaccaa tgacatcatt gaaattgtaa
    10321 cgattcatga agcccatttg atcgtggttc gaacaggaac taccaaaatc tatggaagcc
    10381 tagcagaagg tgaatttgtt gaagttgaag gttatcttaa tcagaagcta gatgttatcg
    10441 ctgaaaaaat tattgtagat gatgatcgga atggatattc tcgtgattat catcattatg
    10501 aatcaaagcg aagtgatgat gatcgttttg gagttatcaa ccgcaattac gggaaacatc
    10561 atgggaaaaa gaacggtcgt gataaagacg aggaccgtga tgattaatac tgtgtaatgc
    10621 tgagatctaa ttgcgatcag tgttaatctt tgatgagttt atggcgtgat cgtaatattg
    10681 acggtattgt gaaatgtgcc attggcaata ccgtcttgat tgtcatcgac agcaaatgtt
    10741 atagagtaat tacccggttg tagggctttg agcgagcttt tcatgatgat gaattgattg
    10801 atcgcattga attctccaac atgcgcccgt atcggttgtg aggaaggaat aaattcaaat
    10861 cgatcattta accagaaagt ttcggttggg gtgatcgcac gtatccaata ctctgcaggg
    10921 ttttctagaa gtacccccgg atctaaagat actgtcagta aaagtgtatc gtttctggaa
    10981 agttgtaagg ttcccgtttg attatttgct gcaatcgtag gactcggata tgagagtttc
    11041 ggtggattgg gtgaagttaa gtcattaaag taccattgca cattcgtatt gaaatagatt
    11101 ctactccggg taatcgcaaa atgttttgaa ttcagttgtt cagctcgggt ttcgcaaact
    11161 gcaaacgatg aacgactgtt attgaagctg acgacaatat ctgcatcatc cgtaaatcca
    11221 atgaacgtta gagctaacgt atagtcatca ggtgtgccag caaatctatc tagcccactc
    11281 atggcaagtt ttaatgttgc aacatcatct gctgtgaggg ttcgttgtgt ttctccagcg
    11341 atgataccct gctgcataac ggcttcagta ttttggtaac caagcagatt cgctacagtt
    11401 cgatcaccat tggcagcgaa ggtatgccct accggtaaat cacttaaatt ccgactataa
    11461 gtggtaatat caattttttc tgcgatagta aaagggttat tattttttct cctaaaccaa
    11521 tgcaagtttg catcatcacc tcgtaaatca tcatttgagc cagcgatacc atcgcttcca
    11581 ctattcagat cgtatcggtt atttgagccc cgagtggttt gggtatagtt acgattttct
    11641 cccgaaagcc ctgattcaga agataaattg ggatgtgcaa gacctaagca atgcccgagc
    11701 tcatgtaata ccacggattc aaaatcgaaa tgattgaacg gaattctttc atcgctaacg
    11761 atattgccaa gcgtgggaat taattgattc caagtattaa ttgcattttg aatcggtagt
    11821 gctatttcag tgccatgaag tgaagaaggt gagatgccaa ctgtaacgat tagatttgtt
    11881 ccttgaccct gataaccttg aggatgggtg ataacgtctg ctgtttcatt atcttctgca
    11941 aataagaaag taccggctac ggtattcagc attagcccat aggtaaatat tagtaaaatg
    12001 atcgtgctga tgtccttcat tatgtacttc gacgactatt ggtaaaaagg attaattaag
    12061 cgactttagc tcagaagatt ccagaatatt ctcaaatagt tctctaactt ccatcaattc
    12121 gtgcagttct tctaattctg gaaagttttc tactgtccat tgtagtgcgt accaagtgat
    12181 tcgctttgtc gtattccaat tgttttgatc aatactggca gttggcatat atcggtttgg
    12241 ataaatttct atgatgattg taggcgcatc tgaaacacgt tgtagcaata acgtattttt
    12301 ttcctgttga tttttagcag ttttcagacg atgctgaatc tcagtttcat tgaaattaag
    12361 taggcttggc gaggttaatt gattccacaa ttgattcaac gcaactggat caaggtgtcc
    12421 agagaatatc cctgattttt tataataact aggacgatat acttttacgt aaccatctgc
    12481 aaagattgtc acggtgatat tctcgttaaa atttcgagtg ctactttgct tttcctcgaa
    12541 atgggcaatg atagtctctg atttcagtaa attttctgca tgtgaaatag acagtccaat
    12601 aattagcaga actaacccaa gtaacatttg caatgtactg gagattttta acactagggg
    12661 aaagcttcga aataatcgag tcatttaaat attctcttgc aaaataatag tcggtgcacc
    12721 tagagattta gagtggtttt ttgcttaatt tctacctcga taaaaataaa attaggggtt
    12781 aatttaattt cccgattgaa ttaagccact gaccataatg tcgttaatac tgacattcta
    12841 aacttagttt aattcaaggg agccaattgt gatattgctt gcaagttcat atgaagaaat
    12901 gctagatgaa tggagaagaa gcttaactga tgtttcgtcc gttctttgca tcaatgatca
    12961 tgaattgctg aaccatgaat tagcgagaag aaaaccgcca gtacttctaa tagaccatga
    13021 cttcttaggg aatgatggtt caaagaaaat agagggttta cttgccttaa gccctaaaac
    13081 tcaaatcatt atatcaagcc caggtatttc agatgaaatg gaatggaaat tatttaagtc
    13141 gggaatcagg ggatgttatc gtgattcgat tgaacctgga gaaataaagc gagttgttaa
    13201 agctgtttgg caaggagaat tgtggattcg gcgcaagctg gttcatcata tgttggatga
    13261 gctggtggtt accacaaaag agaaaaaaca tattgagcgt gctgttaata atctgcttga
    13321 aaatttaacg aagcgagaat atgaaattgc tatcttggtc ggtcgtggtg aaagcaataa
    13381 acagattgcg agaagattag atataaccga gcgtactgtt aaagcccatc taacagaaat
    13441 ttttcgtaaa ttagcaatta ccgatcgaat aaaactagcg ctacttgtta aagatactgc
    13501 agtctcttca aatcagactg tgcttcatca ttctaataat tttcctatag cttctgccta
    13561 attaactacg atttagccag ataaacggtt agatatggat aagatgagtt ggatcttatc
    13621 aaggcacaag cgtaatattt ttacctaaaa gccgatccga taacttagca atgcgcagat
    13681 tattgcgaaa atcattcatg gtcatgccga ttccccagag gttcagaaat tttccactcg
    13741 ttgaatcaaa gatatgatca cgtaacatac tttcttggat aaaacctaag gctaaagcgt
    13801 tttgttgaga tacggtatta tcttcataaa cgatagacgt aattttgttc aaattgactt
    13861 tattaaaagc aaaatcgaga actaataaag tcgcttccag ccctaaccct tttgcgtgat
    13921 tgtttgtatc tggcaagcca ataagaaact cggcacggcg gtgagcaaac tcaataccaa
    13981 ccagatttgc gataccgata ggttgattcg ctcctttctt cataatgacc caatcaatgg
    14041 atttaagttg gcaaggatgc attttattcg cttcgtctaa ttttaaggca agctcctctg
    14101 cgcgttgatg gcggggaata tagtgattgt attgccccat aaactgggta ttttgataac
    14161 attggcgaag atagcttgca tcttctttac aatagcgacg taaataaagt ttgtttccct
    14221 caagtggagc gagccagatc atggcaactt cctggtgatt taagattgct tgagtaaagt
    14281 taggacggag tgcaattgct ttgagaaaat agtttcttgc ttcctcgatg cgacccagtg
    14341 ttttgagtgc ctggccgaga ttattgtaag cttctggata attttcattt agcccgagcg
    14401 cattggaata gcttttaact gcttcctcca ttttgtttat tttttgaagc gcattaccta
    14461 aattatttaa tgcctgcgga aaattagggc gatattgcaa agcgtttcga aagcagccaa
    14521 tggcagcttc tatacgattt tgtgcaagat aaacattacc cagaatatta tggacatctg
    14581 cttgattcga gttaagtgct agcgatttct cgcagttaat taacgcatcg tcaatattgc
    14641 cttgttccca taatgcattt gcgtaattac caagaaaatc agcgcgttga ggagcaacgg
    14701 ttattgcctt gataaagtaa ttgtttgcca attcgtattg ctttgtttgc gcgtaaataa
    14761 tggcaagtaa atggagcgta tcaggttggt taggcactaa tgcacaaact ttcaggcagc
    14821 aggcttccgc atcctggaaa ttgccttgct gatgacaatg cgccgctttc tgtaacaaat
    14881 tagaaatttg agactgagac gtagtcattg tgaacagggt attattaatt tcgctgactt
    14941 accctaaagg tttggatatt tcagcgaaat tagacgtgtt actttctaac ccagcggaag
    15001 aatgggtaga ttgaattcgt taatacccac tgtcgaggtt tggaacgcat caccggtagt
    15061 aaaatcaagc gtatcagcat cttggatacc tttataagta ttacccgcga gatctttaat
    15121 aacgccttcg gcaatctgaa tgttgtaggt tgtatttggc atcaagtctg tcgatggatc
    15181 gattgttacc ttgctgccac tgatcgtaac ttggctggta tcgttaattg caaaggtgtg
    15241 tgtgtctgag gtatccgatt catttgtgat gatgatgtta ccaattccag ctgttatttg
    15301 ctcatcaaag ctcaatgtga tatttccatc cgtaggaaaa agaattgtat catctgctgg
    15361 cgtactgaag gataggcgtg ggtttgatgg aatcgtcgtg aagttaagcg ttgtgttatc
    15421 cgagatgcca atataagcat gacccgcaag atcagtgatt gcagtattat cgatttgaat
    15481 actatataaa gtattcggaa tcaaatcagc tgtcggatta atgatgacat taccaaaatc
    15541 atcaaatgat acttgagtta tgtcatctac tgagatcatg cgactatcag ctggattggt
    15601 ttcattcgtg atcgtaatat ttccaacacc tgcttttacg gtttcatcaa aactaagcca
    15661 aagattatca tcaattttga aatcggtgga atcatcccag ggggagctac ctattaagaa
    15721 tgggttaagt gtcgtcgtga aatttagtgt tgtatcatcg ctaatgccta ggaatgggtc
    15781 acccgctaaa tcgttaatta caccactttc gatttcaata tggtaagtag tattgggtat
    15841 taaatcgtct gttggattaa tagtaacaat agcgctatag tattgatcaa agctgacctg
    15901 gctagtgtct gtaattggaa tgatgcgtgt atctactcca ttgctgatga tgatattgcc
    15961 actaccagca attatacttt cgttgaagaa gagctcgatg ttattttcta cttggaaatg
    16021 attgaaatca tctggtgggt tgctccagtt cagcaaggga ttcgttggaa tggttgtgaa
    16081 attgagcgat gtgttgtcgg agataccagc atatgcattt ccagctagat cgataattgc
    16141 accggagtct atttgaatac tatattcagc attggaggct aggttgtcca taggattgat
    16201 gatgacataa ccagaatcat caaatagaac ttgagtagca tcattgacag agattacccg
    16261 actatcagtt gaatcagctt cattcgtaat cgtgatatta ccaatacctg tttttactcc
    16321 ctcatcaaag ctaagaacga tatcactatc gacctggaat tcactactat catcccaagg
    16381 atagctaccg atcagtaaag gattagaagg gatggttgta aagttgagag tccgactgtc
    16441 attaatacca aggtatgggt tgcccgctaa attggttatc acgccacttg cgatttgaac
    16501 attgtagtca gtgttaggaa ttaaatcagc ggttgggttg atggtgatcg ttgagccatc
    16561 tttactaaaa gaaacttgat tggtgtcatt gattggaatg attcgtgtat cgactccatt
    16621 actaattatg atgtcaccgc taccggccat tactgtttca tcaaaataaa gctcaatatt
    16681 gctatcaatt ttaaattgcg ttgcatcatc ccacggatta ctaccaatta attggggaag
    16741 tgaaagtacg gtagtaaagt ttaaagtatt ttcatcagca atccctgcaa aagggtttcc
    16801 aactagatcg gtaatggccc cagcacccat ttggacattg taagtggtat taggaatgag
    16861 atcatcgtgt ggattgatcg ttacataatg acttcccttg ctgctggtga aactgatttg
    16921 gcttgtgtca gttgcatcga tgacacgcgt atctaaccca ttgctaatgg taataacgcc
    16981 cgttccagct ttgattgctt cgtcaaaata caagaaaata ttttcatcat tgccaaatgt
    17041 cgtttgatca tcggtaggtc tgctagacaa aaatgaaggt gctgtattat cgcccggagt
    17101 aattaccgta aaattttgtt gggcgggtaa agacccacct tggttatcga taacgtcgta
    17161 gtttaattcg actggaccac tataatcaga atcaggcgta aataaccatc catcagggat
    17221 tgtagtcaat aatccatggg tagcagttaa gtttgaaata gacaatggat cgccatcggc
    17281 atccgtatac cctgataaca aatcggttgc caagatactg taaggttgat tgacgattcc
    17341 atcactcaat atggttggtg cgcctgtaag ctcgggggca tcattaacgc ccgcaaacgt
    17401 caccgtagca gtggcccaac tcaggggatt gttgcctttg ccttgacgca ttgcatagct
    17461 gaaggtgtca actaaatttt ctccttgagg taaggccttt agggcagacg ctaaatcggg
    17521 tgttagctca taggccactt taccctcttc ggttatccag atatttgccc ctttttcgct
    17581 taggttaata gcaccgattt cgtcgggtga caagagatca ggagaagggg gggcagagct
    17641 gctcgcgcta tcatcaattg accagagtgt ttgagattgg ccaccggaat catttgccag
    17701 gacgtctaat acataggtag tgttattgct agaaagattt tcaaaactat cgttattggc
    17761 tttggagttc ttcgtaaagc tagctgttga taaaacattg gtctttgtat tagtcgccat
    17821 aaacctatcc tctaaaatag aaaatgatcg agtttgatat aaatttatcg aagtggggct
    17881 atccatacct gaatcagcag atgtttccac aatgaaaaat ctatgtacat agaatatcag
    17941 agatcttaca aaataatgta aatttcatat ttcagaaaaa ttctgagcta atttgtggga
    18001 aaggttgagc tgcttgcgat ttttccgcga atgagcgtgt aatttagctg ataatcacag
    18061 taagctacca cgagtaggta aaggtggcct tcctgctcgt tcccgtgaat atttgtttac
    18121 attaattcga taaaaaactt agaatgcttc atttctcatt tgttttcaca ccatagtatc
    18181 tcgcaataac tttcaggaag ttggttgctt gcaatatatt cctacccccg gtaaaaggtt
    18241 tattgttttc aaccatgtcg ccccaaagga acgcgccaaa ccccccgatc ttgtctgact
    18301 ggttaagaat ccaaccataa aatcctattt ggttgatcag cgtatcgata tctgcaaaat
    18361 gtccatctgc caggaatgtt cctggtatca cgaacaattt tcgtccgata gcttgttgag
    18421 attccatcgc tagcatggca tcccacaccc atgtcccgta ttcaaactgt gagtaataac
    18481 catactcatc agagccacag aattcgatac ctccataaca atcaaatccc aggtattctg
    18541 catcatctaa cataaccaca ttttcttcag gataggcttt cttttgtagt acgagttcag
    18601 cccaggcttc gatatgtata atccctgaac ctggtaaatg tttgcgcagt aattgcccag
    18661 caattcgaaa agttgataag gtttctacat atcgattagc aacatcgaga caggcttctg
    18721 cttttccctt ttcttcacag gcacgtcgga cccaccaaaa cggctcatcc attaaaaata
    18781 gaatttctgt atctttgtgt ttagctttag caattgcttc gacaatgctc gtaacatcgt
    18841 gatggtacat gcccgtttgc tcatcgagca ataaccgact aatgctaatg actggaagca
    18901 ttctgccttt attttcatct agatactgcg ttatgtctct cggagatgcc ggagcaaatg
    18961 caaaggcatt actaaacgaa ttgacctctt gaatttgcga tttttcagtc acaaacgatc
    19021 caatgatttt ttgtgaccga tgtgtattaa agtaattagc aatcgcaata ttcgagacta
    19081 gcatcatcag tatgattatc gttagcctgt ccatcgtcgt tctccttcat cagaaactta
    19141 ttcattgatg aagacgatgg tagatttatg gcaagttaac gtcggtgcat aaactcactc
    19201 aaggaccaga tataaatcac actttatctg ctaactattc gaagtattgc aaatataaaa
    19261 ttgaaatgat tttctcgatt atttatattt acccaaattg taactattcg aaagcatcat
    19321 cgtgacgcca caactgtgca gccccaagaa caccggagct gtcgccatga atattgcgca
    19381 acatgggtgt tataaactga tcattaaaca catactgcgc taccttagct cgaccctcgg
    19441 tatacaatcg atcgatatta gataatccgc cacccaacac gatcacatca gggtcaagta
    19501 tattgacgac tgttgccatg gcacggccaa atcgatcaaa aaaacgttgc atagcagctt
    19561 ccgccaagct atcaccttgt gctgcaagcg ccacgatccc attggttgta atttcggtat
    19621 taccgccatt acgataaaaa tccgctgcca ggccagggcc tgagagaaac gtttcgacac
    19681 agcctttctg tccacaatag caatcaggcc cgtttgattc caaaatattg tgtccccatt
    19741 caccaccaat gtgctgatgc cctgagtgca attgcccttt gaatacaata ccgccaccca
    19801 cacccgtgcc catgatgacc ccaaatacca cattgtagcc tgttccagca ccattgagcg
    19861 cttcacttaa ggtaaagcaa ttcgcatcat tagcaatacg cagcgggcga ttcaatagtg
    19921 cttgtagatc ttcaagtaaa ggctgcccat tcaggcaagc ggtgttggaa ttcttcatct
    19981 tgccagttac agccgagata gcgccagggg tacctattcc aactgaacag ggtttaccga
    20041 cctcgttctc aagcagcaat acgagttcgg ctattttgtg caagattgcc tgatagcctt
    20101 ccacctgacg tgtttcaatc cgtttcctca gtaattctgt gctattgcga tccagcacaa
    20161 ccccttctat ttttgttcca cctaaatcaa tgccaattcg catttattta tccttaatga
    20221 aattaattca acgttgctcg tcatgttgct attgggcttt taatgaacta ctctaaactt
    20281 attttactga aaacagatac tgaggtgatt gatgaaattt gaaacgattg ctatccacgg
    20341 gggttatagc ccagacccca cgaccaaggc agttgctgtt ccaatttacc aaacaacttc
    20401 ctatgctttt gatgatacgc agcatggcgc tgatttattc gatttaaagg ttacgggtaa
    20461 catctataca cggattatga atccaacgac tgccgtcctt gaacagcgta tggctgagct
    20521 ggaaggaggt atcggcgctc tcggtctggc atccggtcaa gcagcgatca cttactcaat
    20581 tttgacgatt gcagaagcgg gagataatat tgtttcagtc ggtacgttat atgggggaac
    20641 ctataattta tttgcccata ctcttccgca atatggcctc accgtgcgct ttgctgacta
    20701 ccgaaaccca gagaatttta aagcaatgat cgacgaaaaa accaaagcac tctattgcga
    20761 gtctattggt aatccactcg gtaatgtcgt ggatattgca gcgttagcgg aaattgctca
    20821 tgagagaggc gttccattga ttgtagataa tacggtaccg actccttatt tatgccgccc
    20881 ttttgagtac ggcgctgata ttgttgtcca ttctttgact aaatatttag gggggcatgg
    20941 caacagtatt ggcggtgtgc tgattgattc aggaaaattt ccttgggcgg atcatccgaa
    21001 tcgattcaaa cgtctgaata cccctgatgt atcctatcat ggcgttgttt atacagaagc
    21061 attgggaccg gccgcctata ttggccgtgc acgcgttgtt ccattacgca acatgggggc
    21121 tgccatttca ccttttaata gtttcttaat attacaaggc attgaaacgc tccccgttcg
    21181 catggaccgt atctgctcga atacactagt cgttgcgcaa tatctcaaaa cacaccccaa
    21241 agttagctgg atcaagtatg cgggcttgcc tgaacaccct gagcatcctc tgacacaaaa
    21301 atacatgagt ggcaaagcat caggcatact gacttttggc gttcaagggg gtattgcggg
    21361 cggagcgcgc tttcaagacg cgcttaagct cattacccgg ctcgtcaata ttggtgatgc
    21421 caaatcatta gcttgccatc cggctagtac cacgcatcgt caattgagtg cggaagaaat
    21481 gcaaaaagcc ggggtatcgg aagatatggt acgcctatcc attggattag agcatatcga
    21541 tgatttaatc gatgacttgg agcaagcgct tgctgcggta tgatgcaaac gctaaattct
    21601 tcatctggat gaccctttag actttatcct tgataaatca gttttcagca aaacctctct
    21661 ccaagcataa ctattgccct gaagtgtgat atttcacaca gcgttacgat ctcaataaaa
    21721 ctagcatgaa actgctcaag cgcgattaag cttgagtaat cacaatacac gatcttgaaa
    21781 tcgtaactat ataaagcaag gagctaattt atgaaaaaat atattcattt tccatcgact
    21841 ttcaaaaaag cgatgcttgg attgagtatc ctaataggca gtttatcaaa tctaggtcat
    21901 gcacaagatc aacttggctt acttccactc tccagtttac gggttagtga cgcacaagcc
    21961 tatgaaacat gtccagctgt aaatccatcg attcggcatg ccgattttgt ggtaatttta
    22021 cagcgccagg ccaatgggcc catcaacact gagacagtag agttttcaac ccacgatggg
    22081 acagcattat cgggtgtaaa ttatgtgcct gtaagtggaa aattaacgtt tctacccggt
    22141 gaaacagtca aatctgttcg tgtcataatt ttgagcagtc aaaatacaga cgatagttac
    22201 tttttcttaa cgctttccaa tccgagtatc caaacggtac ttcgtgattt taatggaaca
    22261 gccagtattg ctcatcttga tccagaaaac gaaacttgtc gaaatcgagt cagcttaaat
    22321 gctgatccaa ggctaccaga cccgccccca gtaccaaaat aattctaagg caggactgga
    22381 tcaattcatt tccatcgaat tgatccagca agctaatgcg cttacatgca atgtttcaat
    22441 acaaaatttt gatgtaagcg tgctttaagc actggctgtt gcaaaatagg ctcctgctga
    22501 atgttacgct gaatataatc gattaaattc atgatacgta ccgtaccact ctgatcaatg
    22561 gccctaccac ttagtccttc caataaatat tgagtaaaac aactatgtct gcggtggggc
    22621 agtgcatatg aattactgtc tcctgtggta gcagccaaaa tagcccaatt tcggttattt
    22681 tcttttggta aagaccgact caaatgctct gcaatgctac ctgctttgca gcaatcaaga
    22741 accagaaccc attgcaaggc attgattgcg cccaatttgt ttgaaaattt ttccatgctg
    22801 attgcgctcc cctctaactg agttagatcg tcagctttcc catcaaacgg aagcaagtaa
    22861 taccaaaacc gaccaggttg ggctgcacgg acaccatgcc cagcgaaata aatgaaaacc
    22921 aaagtatttt catcagtttc cccggctaaa gcgtccagtg catttatgat tgccaattca
    22981 gttgcttgcg tatccaacaa taaagtttgg ctagaaaacg ggtacgccaa actcatttct
    23041 gcaaataaat tggcaatatc ttccgcatca cttgtagcac gcaatggtgg aacattttta
    23101 taatggtaaa ttccgattat caaaactgcg tagcgcataa tttcagctaa gtctagcgag
    23161 ttaaatctcg atacctcgct cgatctgaaa aaattgctta atcgattcat gggttgtagt
    23221 gatttcaagc ttctcatagt gagtggaaat atctggcacc tcgcttttcc ctcgaatcga
    23281 gatatgcggc tcagtcattc caccacaaat ttgatgaagc tgttcattcc caagatcttt
    23341 ctcaaaattt tctgtatgaa tattatcacc gctgatgtcg agttgatttc gtgaggtgat
    23401 tagatagatc ccagttgtaa gattgataat gccagtagcg ctttgctcag gcacattcga
    23461 atcataagca aataacattg catgatgatc cagtgggtca atcggttgaa tcgaaacagt
    23521 agtcggtttg agaacatata gagcagtacg catcgtaatt cctcttcatt aactttgaaa
    23581 ttttgcttaa gtaatagatt tagctacctt cctcatatta agaatggcgt atgttgaatt
    23641 agattagatc ctgctactca acctgaccac cctcatccgg atcatcttca taaataaaac
    23701 cggattgtgt cagatgctga atcgatggct gaaacacgat acttacttgt tcaggagaac
    23761 ccaagcctcg tggagtaacg aactctgccc ttgctgacaa agaagtgagg cttgtaatta
    23821 cgaaaattat agaaataatt gcagttttag ggattgctaa gctcatgttc attattttct
    23881 ccttggattt cgtctttcaa ttagtatggg ttattacaaa accaaaataa cctttaatga
    23941 atttacaatc tgtgagaaaa ctcacaccga aatgctgagc ttactgaatt ctcaatgagt
    24001 taaggctggt aaggaatttt tttgatgctg ctgctaaccg ttcaaagaaa taatggcaga
    24061 atttacctta ccttaccttc cgcctccctc caagcttgtt tttctgtatt aaaaccgcgg
    24121 ttaatcactt taatactacc atctgcgttt ttactgtaat tggccgtcac ttgttctagg
    24181 ccacgttcaa agctgtggtc tagccgtgca atttcgtacc aagtgcctaa atattggttc
    24241 gcattcacgc catttactac agttacgttt tcaggtatgc cttggcaagc aaatagggaa
    24301 aaggttaata acaatatgaa taattttttc atcttagccc gctttatctc ttgatgacgc
    24361 ctgttagaaa tcggtctaac tgtgctgcaa atgcttgcct attcgattga ttaaacgtgc
    24421 ttggaccctt tgtttctatg ccgctggcgc gaagttcttc caataagttt cgcatgctta
    24481 agtgctgctg aatgttttct ttagcgtaaa actcccccct cggatttaac gcatatcccc
    24541 ctctttcgat aacttgtgca gccagcggaa tatcggcggt aatcaccaaa tcacccgctt
    24601 ccagtaactc cactatttta ttgtctgcga cgtcaaaccc gttttcaacc tgcatagtgc
    24661 ggatattggg cgagggtggc acatagataa gcttattagc taccagcgtg gtcatgatgt
    24721 ttgtgcggtt tgcagcttta aagagtattt ccttaatgac aacagggcaa gcatctgcat
    24781 ctacccacac tttcataaga gctcactttt cgtacaaatt gtgatgtaac gcagtgcccg
    24841 cacgctcggc gcaatacaac aaactgtctc gcgttctgta ctccgtaggc ttcgtactcc
    24901 ttcacatgtt gataaattat tgcgcattac tttttagcta atggcctctg ccaccgccgc
    24961 ggtatcgcgg cggtttgggg ttgaataaag gcatttgcac ttgtgcttgg tggcgcgccg
    25021 cttctgaaag taatttacct ttttggggtg ctggagatat caatttaccc tgcccatatc
    25081 ctatcgcggg tttgctgtta gatgaatcat tggcatggga gttacgatct cgcccttgcg
    25141 tttgactccg cttgggctga ttttgcttat tctgtttttg tttcgattgc ccctgattgg
    25201 gctgtccttg cttaggcatt cttgagcgat taggttgttg ctcacgaaat aatcttggtg
    25261 actcagtctc tagtttagcg ggcggtataa agccttccac tgctactttt gggatttcgc
    25321 gtttgattaa cttttcgata ttttttaaaa acttgatttc ctcgacatca accaaactaa
    25381 ttgccgcacc agtagttccc gctcggccag tgcggccaat tcgatgcaca taatcctcag
    25441 ggacattcgg tagctcaaaa ttgactactt gcggcaattg gtcaatgtcc aagccacgtg
    25501 cagcaatgtc tgtcgccact aacacaggga tttggccaga tttaaattct gccaatgctt
    25561 tggtacgtgc gccttggctt ttgttgccgt gaatcgccat cgccgcaatg ccgtctttaa
    25621 ttaatttatc tgccaatcgg ttagcaccat gcttagtgcg agtgaatatc agcacttgct
    25681 gccaattgtt ctgcttgatt aaaaagctca ccaaatcgcg tttaagtgct tgttcaacca
    25741 aatgcacagt ctgctcaacg agttcagttg ccgtgttacg gcgagaaact tccacaaaag
    25801 cagggttatg cagcaagccg tcagccagtt gtttaatttc atccgagaac gtggccgaga
    25861 acagcaggtt ttgacgctgc ttgggcaaca tcgctaatag ttttttaatg tcacggataa
    25921 aacccatatc caacattcta tcggcctcat ccagcactaa aatctcaatg ccggataaat
    25981 ctacattgtg ttggttagca tggtcaagca aacgaccagg tgtggcgacc agaatatcca
    26041 aaggtttgcg caagcgcgaa gtttgcgggt tgatgttcac accaccaaaa atgacagtag
    26101 aggtgagttt taaatatttt ccgtagattt taacggattc ttctacctgt gcggcaagct
    26161 cgcgcgtcgg tgtgaggatc aagcagcgtg gacgatgttt ttctgccggg tatttaacag
    26221 gcgtgttgct gagtatgtgc agcaaaggca gcgtaaagcc cgccgttttg cctgtgccag
    26281 tttgtgcgcc agccagcaaa tcaccaccgc ttagtacttg tggaatagcc ttggcctgaa
    26341 tgggggttgg tgtggtataa cccgcatcgc taacggcgcg cataatgggt tcagataaat
    26401 ttaagtcagc gaacgtaatg tttgctgagg gagttttgaa tgacaaagta aattcctaaa
    26461 aattatagtc catgagttgg cctgttacct ttgaggcaac acccatcgag acaaacaaat
    26521 aggtgaggta tgctgccaag aatggagtct atcgattaaa cagttagtaa atttttatag
    26581 tattgatgcg aaaattatgt tggcgataaa taacccaatc tttcttgctt gtcccgtcag
    26641 ttatagaaca cgatatattc ggtaatttcc tgaatggctt gttgtcgtgc tttgagttct
    26701 cgatgatgca ccaattcggg ttttaatatc gcccagaaac tttccattag cgcattgtca
    26761 tagtagcccc cttgcaaccc atggaaatga tcatactaaa ttgctgtaat aaattcggac
    26821 aatcgcacgc acagctgtct accgtgaaag gggaactaga tgtggagttc tcattttcgt
    26881 cagattttaa tctttggatt gcggattcag catggcgggg ggtattaatt tcccaagagc
    26941 taccaaaagc gtactctttt gatcttttct attttcagaa accccctaaa ggtaggttcc
    27001 taagggtaaa taaatcaacc gaatatgttt ataggtaacg gtgaatgatt atctggcgga
    27061 gagagaggga ttcgaaccct cgataagctt tttgtgccta tactccct
//
LOCUS       DAOQLJ010000013        25969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_624_length_25969_cov_6.625068, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000013 DAOQLJ010000000
VERSION     DAOQLJ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 25969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25969
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_624_length_25969_cov_6.625068"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="PK522_01995"
     CDS             complement(<1..1314)
                     /locus_tag="PK522_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HNC40536.1"
                     /translation="MADNVQNQSIALAHVRKNEEGKWKEHLLHEHLERVSYLAGQSAT
                     TFGSEDWARIAGLWHDLGKYREKFQQYIKSASGYDAEAHIEDATGRVDHSTAGAIHAI
                     EKLGLQGRILAYLIAGHHAGLPDWNNAEGSQSVLFQRIQNGKQNGYLQEALDAMPPAE
                     ILNQSRPQSLPPNGSLALWLRMLFSCLVDADFLDTEAFMDDKRSGLRSWYPSIVALKS
                     AFDQHMADKAANAKDSDVNRIRAEVLRQCREKAKLPSGLFSLTVPTGGGKTLSSMAFS
                     LNHAVHHGKDRVIYVIPYTSILEQTAEIFRSIFGNDNVIEHHSNLDPDREDARSRLAT
                     ENWDAPIIVTTNVQFFESLFAAKTSRCRKLHNIVNSIVVLDEAQLLPPEFLAPILHVI
                     QDLTNSYKVSFVLSTATQPAFSPRPKFTGLKNVCELMDDPERLYVD"
     gene            1588..1866
                     /locus_tag="PK522_02000"
     CDS             1588..1866
                     /locus_tag="PK522_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HNC40537.1"
                     /translation="MARIVKCIKLGHEAEGLDAPTYPGELGKRIFENVSKQAWGQWIK
                     HQTLLVNEYRLNLADIKARKYLAGQMEAYFFGEGVDKPTGYVAPSDNT"
     gene            1954..4035
                     /gene="ppk1"
                     /locus_tag="PK522_02005"
     CDS             1954..4035
                     /gene="ppk1"
                     /locus_tag="PK522_02005"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNC40538.1"
                     /translation="MNDLSNSVQFLNRELSQIEFNRRVLAQAENKEMPLLERLRFLCI
                     VSSNLDEFFEVRVAGLKEQIKLDAPGFGLDGMMPRQAFKRVSEQIHQLVAQQYQLLNE
                     SILPELEQQGIRFIRRASWNEVQRDWIKAYFFRELIPMLTPIGLDPSHPFPRVLNKSL
                     NFIVELEGKDAFGRNSGIAIVQAPRILPRIIRLPSDVSTEEHSFIFLSSILHAHVGEL
                     FSGMQAIGCFQFRATRNSDLFVDEEEIKNLRIALQGELPQRHLGDAVRLEVADSCPPH
                     MITFLLDQLSLTQDDLYQVSGPVNLVRLMRVPDQIDRPQLKFPLFIPGLPFSSQKKKN
                     NDIFHQIRKNDILLHHPYQSFQPVIHFIQQASSDPDVVAIKQTVYRTGSDSTIMEALI
                     HAASKGKEVTVVVELLARFDEEANINWAGRLEEVGAHVVYGVVGHKTHSKMAMIVRRE
                     EGRLRRYVHLSTGNYHTGTAKLYTDFGLITCHEEICADVNAVFFQLTSLGKASKLKHL
                     WQSPFSLHHQIMDAINREAENAKAGKPAHIIAKMNALLEPGIIRALYAASNAGVIIHL
                     IVRGACALRPGVPGLSTNIKVRSVIGRFLEHSRIFYFRDNNQHHVYLSSADWMDRNFF
                     RRIEICFPVLDSRLKKRIITEGLKPYLKDNVQAWEMDCNGYYKRKVSHRGKQFCAQEA
                     LLAKLVAAQQT"
     gene            4051..5595
                     /locus_tag="PK522_02010"
     CDS             4051..5595
                     /locus_tag="PK522_02010"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNC40539.1"
                     /translation="MTREIELKLTIPPGHIKAIAQLPILSSLSIAPPKKQKLYSIYYD
                     TPDLSLRNQRCSLRIRRIGKQWIQTIKSGGNITAGLHQHNESEIPLNKAQPDLDHLQQ
                     TGVITLAQQRKIIRTLKPIFITQFTRDTHTLQTKDGSIIEFCLDHGKITADHLNDSIH
                     EIELELKSGNSAQLFQLALDIAQLTDFPIRLENVNKAERGYKLFIGDRTPPTKAASIE
                     INTKTPFLDALSKILSSCLLQLTKNENGVLFSEDPEYLHQMRVAIRRLRSAFIIFKPL
                     LSEQHQSITFFEQELKWLMHLLSPVRNWYVFANEILPPIISSCPDVEPIKVIQKLGQK
                     QRSKYTQQVQTNLQSKRYLAFILKLNLWIYDLRHDIKLESSISIGSFSRSYLLDQHSA
                     ITKNKEKLEKMKPAALHALRIQIKKQRYAAEFFYSLYAKQECFKYYVQLLAILQGALG
                     SINDNYMIDQQLESILSSQKQKKLARPIGVIHGWKAYITTQMKADLKNNWKEFLTMSP
                     FQRNGQ"
     gene            complement(5668..6732)
                     /gene="fba"
                     /locus_tag="PK522_02015"
     CDS             complement(5668..6732)
                     /gene="fba"
                     /locus_tag="PK522_02015"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646216.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HNC40540.1"
                     /translation="MALVSLRQLLDHAAENGYGLPAFNVNNLEQIQAIMQAADECNSP
                     VIMQGSAGARKYAGEAFLRHLIAAAVEAYPHIPIVMHQDHGASSAVCVNAIRSGFSSV
                     MMDGSLEEDAKTPSSYEYNVDVTSRVVAIAHAVGVSVEGELGCLGSLESGMGEAEDGH
                     GAEGVLTHDQLLTDPEQAADFVKKTGVDALAIAIGTSHGAYKFTRKPTGDILAIERIK
                     EIHKRIPNTHLVMHGSSSVPQEWLEIIREYGGEMKETYGVPVEEIQEGIKYGVRKVNI
                     DTDIRLAMTGAIRRHLAKNKSEFDPRKFLKDATAAAKDICKARFEAFGCAGQASKIKP
                     IMLDNMATKYAKGELKAIIK"
     gene            complement(6826..8292)
                     /gene="pyk"
                     /locus_tag="PK522_02020"
     CDS             complement(6826..8292)
                     /gene="pyk"
                     /locus_tag="PK522_02020"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNC40541.1"
                     /translation="MFRRTKIVATLGPACSDPAVLKRMIVAGVDVVRINFSHGTREQH
                     IAFAELTRSLAHSVGCTVGVLADLQGPKIRIGKFEQGKIWLNVGDEFILDANCQLGNK
                     ERIGLDYKELPNDVEEGAILLLDDGRIVLSVNRVKGSEIFCEVVQGGPLSNNKGINRK
                     GGGLSAPALTSKDIEDIKIAADLKADYLAVSFPRSGEDIRLARSLMQEAGGNSLIMAK
                     IERTEAILALDDIMEASDALMVARGDLAVEVGDAAVPALQKRMIRSARANNRLVVTAT
                     QMMESMITNPIPTRAEVSDVANAVLDGTDAVMLSAESAAGQFPVESVEAMARVCLEAE
                     KEYTVNQSLRRSPDTPPVSIEEAIARATMYTAGNLKIRAIAALTQSGKTALFMSRRSS
                     KVPIFALSPQKDTLGKMTLFRGVYPVEFGEGLYDPEVILDLAEDELFKRGAVRDGDVI
                     IMTIGEPVGKAGGTNTMKIVRVGDCRKPLNIDNFEANI"
     gene            complement(8314..9495)
                     /locus_tag="PK522_02025"
     CDS             complement(8314..9495)
                     /locus_tag="PK522_02025"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNC40542.1"
                     /translation="MSVIKLVDLDLKGKRIFIRADLNVPIADGKVTSDARITASMATI
                     KHCLERGGKVMVTSHLGRPEEGVWSEENSLKPVADNIAARLNKPVRLIKDWVNGGFDV
                     AEGELVVLENCRINKGEKKNVDETAQKYASLCDIFVMDAFGTAHRAEASTHGVAKYAP
                     VACAGILLTEELDALTKALDKPARPMVAIVGGSKVSTKLTVLESLSEKVDQLVVGGGI
                     ANTFLKAAGKNIGKSLCEADLVPTAKVLMDKMQQRNATIPIAVDVVVGKKFDANEPAI
                     TKSADTVADDDMIFDIGPNSAQELVDIIMKAGTVVWNGPVGVFEFDQFGGGTRRIAQA
                     IAETQAFTLAGGGDTIAAIQKYDIYDKVSYISTAGGAFLEFLEGKKLPAVEILESRAK
                     Q"
     gene            complement(9567..10568)
                     /gene="gap"
                     /locus_tag="PK522_02030"
     CDS             complement(9567..10568)
                     /gene="gap"
                     /locus_tag="PK522_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNC40543.1"
                     /translation="MAIKVGINGFGRIGRMVFRASIEKFSDIDVVAINDLLEPDYLAY
                     MLTHDSVHGRFSGTVAIEGNTLVVNGKRIRLTAIKDPAELKWNEVGADIVVEATGLFL
                     TKETCEKHLTAGAKKVIMTAPSKDDTPMFVYGVNDKAYTGQTIVSNASCTTNCLAPIA
                     KVLNDTWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGV
                     VIPELNKKLTGMAFRVPTSDVSVVDLTVELNKDATYDEICSVMKSTSQGTMKGVLGYT
                     DQKVVSTDFRGESCTSVFDAEAGISLDKNFVKVVAWYDNEWGYSCKVLEMVKVIASK"
     gene            complement(10638..12653)
                     /gene="tkt"
                     /locus_tag="PK522_02035"
     CDS             complement(10638..12653)
                     /gene="tkt"
                     /locus_tag="PK522_02035"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNC40544.1"
                     /translation="MGAFGDFKAPVFKNLTSAIRALAMDAVQKANSGHPGMPMGMAEI
                     AEVLWIHHMRHNPSNPKWADRDRFVLSNGHGSMLIYALLHLTGYDVSIEDIKNFRQLH
                     SKTPGHPEYGYTPGVETTTGPLGQGITNAVGMALAEKILANEFNRPGFDIVNHYTYVF
                     LGDGCLMEGISHEACSLAGTLGLGKLICFYDDNGISIDGHVEGWFTDDTPKRFEAYGW
                     HVIPNVNGHDPEAIEAAIEEAKKKQDKPSMICCKTVIGMGSPNKANTHAVHGAALGDE
                     EIAATRPHIGWHHLPFEVPKEVYDAWDARTKGKKLESEWNQKFSEYSQKYPKEAAEFT
                     RRVNGDLPKDWSAYVDGVVGRVNAKGETIATRKASQNAIEGLAPALPELLGGSADLAG
                     SNLTLWSGSKGVSSESGGNYIYYGVREFGMSAIMNGLSLHGGFIPYGGTFLMFSEYAR
                     NALRMAALMKIRSIFVFTHDSIGLGEDGPTHQPVEQTATLRLIPNMDVWRPCDTVEST
                     AAWARAIERKNGPTTLIFSRQNLPFQKREADVIKLIDKGGYILSEAADGKPRAIIIAT
                     GSEVGLAMAAQKTLADEGIHVRVISMPCTNLFDRQDKAYQEAVLPKGIKRVAVEAGVT
                     DYWRKYVGLEGEIIGIDTFGESAPAEVLFKHFGVTTENVVNAIKRII"
     gene            complement(12792..14054)
                     /locus_tag="PK522_02040"
     CDS             complement(12792..14054)
                     /locus_tag="PK522_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNC40545.1"
                     /translation="MANNTILVVDDEIGIRELLSEILSDEGYRVVLAENAEQARVCRN
                     QSRPDLVLLDIWMPDTDGITLLKDWANSGVLTMPVVMMSGHGTIDTAVEATRIGAVDY
                     LEKPIPLQKLLSTVKQALRGGQSKPALTISLANLGKGPQIVELKKKLEQIVNLVSPVL
                     LVGESGVGVEHCARFLHRVNTPWVEPEQLSFLADNPVGILEQVKNGLLFFREIGGLNK
                     LEQKGLLLLLGKLEKFNVRLVCATTQPLAELTAQGTYNSKLYEILSNLTINVPSLRTH
                     REDIPELANQLLSTSIETGEVPFVHFSTAALNTLRNYDWPGNLAQLKSVVHSLALTCT
                     GSEISAEEVQQVLTPPLSPTMSATPDIPLNVSLREARDLFEKNYFEKLIDQESGNMTR
                     VAERAGLERTHLYRKIKMLGIKLRGHGG"
     gene            complement(14118..16292)
                     /locus_tag="PK522_02045"
     CDS             complement(14118..16292)
                     /locus_tag="PK522_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC40546.1"
                     /translation="MKYLILLVTGLSVVMLFLLATAGANTDFLGKFYRWLIVLNVFFV
                     LLLLGIVGFLLWRLRKRLKTRVFGSRLAFRLLVVFSLMAILPGALVYGISVQFLGKSI
                     ESWFDVKVDQALEGGINLGQTMLDNLLEELEKKAKVTALALADSPSQTLSSLNQLLAH
                     SYIQEATLFNQDGKAIAFSSLDDVALFPEIPNATAMRHIRMQKSYSAVESIPNKGLFL
                     RVLVPVNVLSLEDDIRVLQVLQQVPSGLAENAEIVQTGYRNYQELVLSKQGITRLYSV
                     TLTFALLLALFSALASASLISEKLSAPLGMLAEGTRAVAQGDFSRRHQVFSNDELGIL
                     TESFNLMTEQLDLARVNAQQSQHEIESARAYLESILANLSSGVMVFDDKLKIRTVNRS
                     AEQILQIPLIYLEGLAIDECVEKEPKFHLLASEIQNGFGSTQSGEWQRQVLHFSESKD
                     QVLLLRGTRLPESSGGGGIVVFDDITGLLQVQRTVAWGEIARRLAHEIKNPLTPIQLS
                     AERIQHRLMDKLSGQDAKILQRSTETIVNQVEALKKMVNEFSEYARIPELEMVQIDFN
                     RLIHEVLALYETSNAKAENSQCPEIKLALSSDLLLIRGDPARLRQVIHNLLQNAQDAL
                     ADTKNGTITVSTDMISGNVRLCIIDNGKGFAEEIKTLAFEPYVTTKPRGTGLGLPIVK
                     KIVDEHNGAIQIENTQPHGAKVTITLPAINAGQVASTMNRLA"
     gene            complement(16296..16919)
                     /locus_tag="PK522_02050"
     CDS             complement(16296..16919)
                     /locus_tag="PK522_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HNC40547.1"
                     /translation="MPHFIKYNGMNWLERLVSMLILGGVLIGVTIQSAHSEGNIKIKS
                     FALEATDAGYQINVDAAIELNSTLEQALEKGIVLYFVIKFALIDTKWYWVDDEVARSK
                     LRIGLSYNALTRQYRLSHRSKSQGFDTLIDALQELGTQHYIPVEEKSELKPNMEYLAS
                     LQVWLDASRLSKPFQLESLSSKEWNLSSEKKEWRTKLPAAPSFSIQR"
     gene            complement(16897..18174)
                     /gene="rsmB"
                     /locus_tag="PK522_02055"
     CDS             complement(16897..18174)
                     /gene="rsmB"
                     /locus_tag="PK522_02055"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNC40548.1"
                     /translation="MMKIQQLAVDSIEIVLSGKNLTLVLQALWHHNPLLSLQQRGAIQ
                     DIVYGVLRYLGQLEGVLSQLVKRPLSDKRLRNLLLVSLYQLHYSKSPDYAIVDCAVAT
                     TRKLTKNKGSAGLVNAILRNFLRNDTALLDLANAHETGRFSHPQWWIDKLRYQYPQQY
                     EAILLANNHHPPMTLRVNRHKTSVIDYQALLNRCEIESCQVWDNALMLSKPVPTQKLP
                     MFEQGYVSIQDAGAQLAAPFLDIRDGMTVLDACAAPGGKSTHLMEVANIQLTVLDNDQ
                     QRLGRVSENFKRLGIEPFQQVCGDAALPSSWWDGKCFDRILADVPCSASGVVGRHPDI
                     KWLRRITDLATFANMQQNILDALWPLLKSEGKLLYVTCSVFFEENGQQVTNFLDKHPD
                     ARLLPIVHNEVNNGQLIPNSSHDGFYYASFYKI"
     gene            complement(18259..19185)
                     /gene="fmt"
                     /locus_tag="PK522_02060"
     CDS             complement(18259..19185)
                     /gene="fmt"
                     /locus_tag="PK522_02060"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNC40549.1"
                     /translation="MRIVFAGTPAFAAIALKALIDAQFQIVLVLTQPDRPAGRGLKLQ
                     ESEVKKVASEYSIPVLQPVTLKSEVTQEQLKSFAADVIIVAAYGLILPESILQLPLQG
                     CLNIHASLLPRWRGAAPIQRAIQAGDQETGITIMQMDQGLDTGDILLQRTIPIDPSDT
                     AQSLHDNLAQLGAQCIIEALGLLQQGKLLAVEQNDEEVCYARKIEKDEAKIDWSLDAA
                     VIDRNIRAFNPFPVAFTSYQSLSLKIWQARIEQKLSGQPGEIVNIDNDGITVACGKHA
                     IVLEVIQRAGGRKLPVKDFLKGNPMKRGEFMV"
     gene            complement(19208..19735)
                     /gene="def"
                     /locus_tag="PK522_02065"
     CDS             complement(19208..19735)
                     /gene="def"
                     /locus_tag="PK522_02065"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNC40550.1"
                     /translation="MAILKILQYPDERLHKIASVVPAITRDTRVLVENMAETMYHASG
                     IGLAATQVNVHQRVIVIDTSETHDQLNVFINPEIITKAGDAEFQEGCLSLPGIFDKVK
                     RAELVTVRALNLQGESFEMQAEGLLAVCIQHEIDHLEGKVFVEYLSQLKQSRIQTKLK
                     KQQRLTKPSVLSMSV"
     gene            19857..20888
                     /locus_tag="PK522_02070"
     CDS             19857..20888
                     /locus_tag="PK522_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNC40551.1"
                     /translation="MRTISIIAMILAISIFSDRAAKAKDIELLANAPERYVVSQGDTL
                     WGIASRYLKDPWQWTEIWDMNQKQIKNPNNIYPGDIIILVRAADGSSKLMFANEKATI
                     KLSPKMRIEKLEAEKIPSIPSKKLKPFLSQPLVIEKNGLDRAPLILGSSDNRVILSTG
                     NTIYVQNMPFDQGMAWQVFRAGKPLIDPEDNDRVLGHEAVYLGDVEVSNFDAISSVRI
                     THSVQEILKGDRLIPASTLHVDDYIPRAPDFPVTGRIISVYGGVTEIGQNAIIALNKG
                     SSDGLEVGHVLAVYRKSTAKLQDSKIISLPDERIGLVFLFRVFDKVSYALVLHSAQAI
                     KVFDAVQTP"
     gene            20923..22026
                     /gene="dprA"
                     /locus_tag="PK522_02075"
     CDS             20923..22026
                     /gene="dprA"
                     /locus_tag="PK522_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNC40552.1"
                     /translation="MNIQPDIESWLTLSFIEGLGDESFRKLLLTFKHPTDILNAEAQL
                     LERYVKKPIAADIANRRVDQKKIIATLKWLEDPANAIITLNDDNYPKRLLNIADPPPL
                     LYLKGQRELLNTPSIAIVGSRNGTPQGIVNASAFAEAASSAGLCVVSGMALGIDTAAH
                     QGALLGSSSSIAVVGTGLDIVYPAKNHQLAHQLAKEGALISEFPLGMPAIGRNFPRRN
                     RIISGISLGCLVIEATLKSGSLITARQALEQGREVLAVPGSIHSPLSKGCHALIKQGA
                     KLVENINDILDEFNYQPLNLANPLATDEAIKQTYAQENTILLSHLDYEATDIDKLCIR
                     SGLTVETVSAMLLALELDGIVASLPGGRYQRIR"
     gene            22084..22542
                     /locus_tag="PK522_02080"
     CDS             22084..22542
                     /locus_tag="PK522_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HNC40553.1"
                     /translation="MFEILIYLFENYFDSENCPDSETLTRKLTLAGFGEDEISQILGW
                     LTDHTKHEAGKYPVTLSQSKSFRIYSSSELEVIDVESQGFILFLEQAGVLNSLQRELL
                     IDRILQMDGSSSCLDKIKLVVMFDLWVQNQLTNHDLIEKLFIASNPRNRH"
     gene            22711..25197
                     /locus_tag="PK522_02085"
     CDS             22711..25197
                     /locus_tag="PK522_02085"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HNC40554.1"
                     /translation="MSKKLIIAEKPSVANDIAKVLGNFTKEADYFENDEYILSSAVGH
                     LLELAVPEEYEVKRGKWSFDHLPVIPPHFDLIPLEKTATRLKTLTKLIKRKDVDTLIN
                     ACDAGREGELIFRYIVRHSNTQKPIKRLWLQSMTPSAIKEGFNNLLNDSDLQPLAEAA
                     VSRSESDWLVGINGTRAMTAFNSQEGGFHKTTVGRVQTPTLAILVEREEAIKKFIPKN
                     YWELHATFSTNSHSYKGKWFDEKFTKSKENAEARAERIWDLANAEAIRLKCLGKSGTV
                     SEESKPAKETCPLLYDLTSLQREANSRFGFSAKSTLGLAQALYEKHKVLTYPRTDSRA
                     LPEDYLETVKETLQSLTASPYHEFASQILVSDWVKPNKRIFNNAKISDHFAIIPTSLE
                     PKKLNEAELKLYDLVTKRFLAIFYPSAEYLVTTRITRVENETFKTEGKILVNPGWQIV
                     YGKSSPSADSDQENLTLAAIGPNATVKTEEIDTIANQTRPPARFNESTLLSAMEGAGK
                     LVEDEELRAAMSSKGLGTPATRAAIIEGLVSEHYLLREGRELLPTAKAFSLITLLRGL
                     KIPELISPELTGDWEYKLRQIEQGQLKRQDFMNKIAEMTKHMVDQAKSHRGETISGDF
                     AVLKTPCPKCGGILHETYKKFQCQQCDFALWKILAGRQFEVDEMETLISQREVGPLLG
                     FRSKMGRLFTASIKLTDTFEMKFEFGNGDDLEAEPIDFSNHQLLGTCPQCNHHVYEHS
                     TNYVCEKAVGPSPTCNFKVGKVILNRVIEQEQIMKLLTHKKTDLLTNFISKKGRPFSA
                     YLVVNADNKIGFEFEERKPSKVRSKKTSKN"
     gene            25310..25939
                     /locus_tag="PK522_02090"
     CDS             25310..25939
                     /locus_tag="PK522_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HNC40555.1"
                     /translation="MFRDRNDAANQLAKQLDHYRDLRPLILAIPRGAVPMGKMIADML
                     SGDLDVVLVRKLRAPGNPEFAIGSIDESGWTYLADYAGQFVHDSQYIEAEKAAQLKTM
                     QAQRKQYTPIRPPFDPENRIVIVIDDGLATGSTMIAALHTLKAKHPKKLICAVPVAPP
                     DTLEKIKPYADEIVCLNSPRDFYAVGQFYQDFPQVSDEEVIQSLKLSRH"
BASE COUNT         7556 a         6112 c         4949 g         7352 t
ORIGIN      
        1 atcgacataa agccgctcgg gatcatccat caattcgcat acattcttta atccagtaaa
       61 ttttgggcgc ggcgagaaag cgggttgcgt tgccgtggat aggacgaaac tgactttgta
      121 actatttgtt aaatcctgta tcacatgtaa aatcggcgca agaaattccg gtggcagcag
      181 ttgtgcttcg tctaatacca ctatgctgtt gacgatattg tgcagcttgc ggcaacggct
      241 ggtttttgcc gcaaataacg attcaaaaaa ctgcacattg gtggtaacaa taataggggc
      301 atcccaattt tctgttgcta accgggagcg cgcatcttca cgatcgggat caagattgct
      361 gtgatgctcg atcacattat cgttaccgaa aatactccga aagatttcgg ctgtttgctc
      421 cagaatgctg gtgtaaggaa tgacataaat cacccgatcc ttaccgtgat gaacagcgtg
      481 attgagtgaa aaagccatgc tcgacaatgt ttttcctcca cctgtcggta cggtcagtga
      541 aaataagcca gaaggtaatt tggctttttc gcggcactgc cgcagtactt cagcgcgaat
      601 gcgattgaca tcgctatctt tagcgttggc agctttatcg gccatgtgtt gatcgaatgc
      661 gcttttcagc gctactatcg aaggatacca ggagcgcaag ccgctccttt tatcatccat
      721 gaaagcttcc gtatccagaa aatccgcatc caccaagcag gaaaacagca ttctgagcca
      781 caatgccagc gatccattgg gcggcagaga ttgtggacgg gattgattga gaatttcagc
      841 cggtggcatc gcatcaagcg cctcttgcaa atagccattt tgtttaccgt tttgtatacg
      901 ctgaaatagc accgattgac taccttccgc attgttccaa tccggcaaac cggcatgatg
      961 cccggcaatc agataagcta gaatacgccc ttgcaatccg agtttttcga tggcatgaat
     1021 agcaccagct gtggaatgat cgactcgccc tgttgcgtct tcgatatgcg cttctgcatc
     1081 gtaaccgctc gccgatttga tgtattgctg aaatttctcg cggtatttac ctaaatcatg
     1141 ccagagtcca gctatccttg cccaatcttc actaccaaac gtagttgcag attgacccgc
     1201 aagatatgaa actctctcta gatgttcatg aagcaagtgt tctttccatt taccttcttc
     1261 atttttcctt acgtgagcaa gcgcaatgct ctgattttgt acattatctg ccataataat
     1321 aagcgactaa ccattacgaa tagtgtatta cctaagatct ttacataaat ttatcctcat
     1381 agaactacta aaaaatcagt tttctctcgt ttaatttcag taatgaatgc agatttattt
     1441 tattgcaacc tctaatatta tttgtttagt cgaaatagaa cgccgccatc acagtaattc
     1501 ttagcttctc cacgcagtta ttagtacaat aaaatgcttg ttaaagaagt tttttaaccc
     1561 taccttctca ttttaactat taaaactatg gcaagaatcg ttaaatgcat caaactagga
     1621 catgaagcag aagggctcga tgcccctact taccccggag aactcggaaa gcgtattttt
     1681 gagaatgtgt caaaacaagc ttggggtcaa tggatcaagc accaaacttt gctggtgaat
     1741 gaatatcgct tgaacttggc tgatatcaaa gcacgaaaat atctagcggg acaaatggaa
     1801 gcttattttt ttggtgaagg cgtagacaaa cccaccggtt atgttgcacc cagtgacaat
     1861 acataaaaac atcacaatca tccggtaatc tactgtattt ttctaggcac caacccatgc
     1921 tgtggatacc gttgaaaaag cagtgcacca acaatgaatg atctatctaa ttcagttcag
     1981 tttttaaacc gagaactcag tcagatcgag tttaatcgtc gtgttcttgc ccaggcagag
     2041 aataaggaga tgccgttact cgaacgacta cgttttttgt gcattgtcag tagtaatctt
     2101 gatgaattct ttgaggtccg tgtcgctgga ctaaaggaac agattaaatt agatgcgccc
     2161 ggttttggtc tagatggaat gatgccacga caagccttta aacgagtttc agagcagatt
     2221 catcaactcg tggcgcaaca gtatcaattg ctcaatgaaa gtatcctacc tgagctagag
     2281 cagcagggca ttcgttttat ccgcagagca agctggaacg aagtacagcg agactggatt
     2341 aaagcatatt tcttccgtga attaataccg atgttaacgc ccattggtct tgatccctct
     2401 catcccttcc cacgcgtatt gaacaaaagc ctaaacttta tcgtggagct ggaaggtaaa
     2461 gatgcgtttg gccgtaattc gggtatcgcg attgttcaag ccccccgcat tctacctcgc
     2521 atcattaggc tgcccagtga tgtcagtaca gaagagcata gttttatatt tctatcatcg
     2581 attttgcatg ctcatgtggg tgagcttttc tcaggtatgc aggcgatagg ttgttttcaa
     2641 tttcgtgcaa ctcgtaacag tgatcttttc gttgatgaag aagaaatcaa gaatctgcgt
     2701 attgcacttc agggcgagct tcctcagcga catttgggcg atgcggttag acttgaagtg
     2761 gccgacagtt gtccgcccca tatgattacc ttcctactcg atcaattaag cctcacacaa
     2821 gatgatctgt accaagtgag tgggccggtt aatctcgtaa ggctaatgcg cgttcccgat
     2881 caaattgacc gcccccaact taaatttcca ttgttcatac ctggcctacc cttttcatca
     2941 caaaagaaaa aaaataacga tattttccat caaatccgca aaaacgatat cttgttgcat
     3001 catccttatc agtcttttca accggttatt catttcatac agcaagcttc aagcgatccc
     3061 gatgtcgttg caatcaagca aactgtctac cggactggct ctgattcaac cattatggaa
     3121 gcactgattc atgctgcaag caaaggcaaa gaagttactg tcgtcgtgga actcttagca
     3181 cgatttgatg aggaggccaa tatcaattgg gccggccgcc ttgaagaagt cggtgcccat
     3241 gtggtttatg gtgtagttgg ccataaaact cattctaaaa tggcgatgat cgtacgtcgt
     3301 gaggaaggca gattgcgacg ctatgttcat ttaagtacgg gcaactatca taccggcacc
     3361 gcaaagcttt ataccgactt tggtttgatt acctgtcacg aagagatttg tgctgatgtt
     3421 aatgcagtat tttttcagct cacgagcctt ggtaaagcca gcaagttaaa acacttatgg
     3481 caatcaccat ttagtctgca tcatcaaatc atggatgcga ttaaccgaga agctgaaaat
     3541 gcaaaagcag gtaaaccggc tcatatcatt gccaaaatga atgcattact ggaacctgga
     3601 attattcgag cgctttatgc agcctctaat gcaggcgtaa ttatccactt aatcgtaagg
     3661 ggggcttgtg cgctccgccc tggtgtacct ggcttatcca caaatatcaa ggtacgttca
     3721 gtgattggtc gtttcctcga gcactcccgt attttttatt ttcgtgataa taaccagcat
     3781 catgtttatc tatcgagcgc agattggatg gatcgaaatt ttttcaggcg aatcgaaatt
     3841 tgcttcccgg ttctggattc acgtctcaag aagcgcatta tcactgaggg attaaaacct
     3901 tatttaaaag ataatgttca agcttgggaa atggattgca atggctatta caagcgaaaa
     3961 gtttcacatc gcggcaaaca attctgtgcg caagaagcat tgctcgcgaa gctcgtggcg
     4021 gctcaacaaa cttaagctcc aaagcctcct atgacacgag aaatcgaact taaactcacc
     4081 attcctccgg gtcatatcaa agcgatagca caattaccta tcctgagttc attatcaatc
     4141 gctcccccga aaaaacagaa actttatagt atttattacg atacacccga tttatcgcta
     4201 agaaatcagc gctgttcgtt gcgcatacgc cgcattggca aacaatggat tcaaaccatt
     4261 aaatcaggtg gaaatattac agcaggtcta catcaacaca atgaatctga gattccctta
     4321 aataaggctc aacctgattt agatcactta caacagacgg gtgttatcac ccttgctcag
     4381 cagcgcaaaa tcattagaac attaaagcca atctttatta cacaatttac gcgtgatacc
     4441 cataccttac aaaccaaaga tggcagtatt attgaatttt gcttagacca tggaaaaatt
     4501 actgcggatc atctcaatga ttccatccat gaaatcgagc tcgaactaaa atcgggcaat
     4561 tctgcccagc tgttccagct cgccttagac attgcacagc tcaccgattt tccaatccgg
     4621 cttgaaaatg tgaataaagc agaaaggggt tacaaactgt ttataggcga tcgtacgccc
     4681 cctaccaaag cagcatccat tgaaataaat accaagactc cattcttaga tgctttaagt
     4741 aagattcttt cgagttgcct cttgcaatta actaagaatg agaatggcgt cttatttagt
     4801 gaagatcccg aatatctcca ccaaatgcgt gttgcgattc gccgacttcg ctccgctttt
     4861 attattttta agccactact ttccgaacag catcaatcga tcaccttttt tgaacaggag
     4921 cttaagtggt taatgcactt gctaagtcca gttagaaatt ggtatgtttt tgccaatgaa
     4981 atcctaccgc caatcatctc tagttgccct gatgttgagc caattaaagt tattcaaaag
     5041 ttgggacaaa agcaacgcag taaatatacc caacaagtgc aaacaaatct ccaatctaag
     5101 cgctacctag cgtttatttt aaaactcaat ttatggattt atgacttgcg tcacgacatc
     5161 aagctagaat cgagtatatc gattggatcg ttttcccgat cctacttact tgaccagcat
     5221 tctgccatca ccaaaaataa agagaagctg gagaaaatga aacctgccgc cttacatgct
     5281 ttacgaatcc aaatcaagaa acagcgctat gcagcagaat ttttttactc actctatgcg
     5341 aagcaggaat gtttcaaata ctacgttcaa ttactcgcta tactccaagg tgcgctggga
     5401 tcaatcaatg ataactatat gattgatcag caacttgaat ccattctctc atcacaaaag
     5461 caaaaaaagc tagcacgacc aataggcgtt attcacggat ggaaagcgta tataactacg
     5521 caaatgaaag cagacttaaa aaacaactgg aaggaatttc ttactatgtc accatttcag
     5581 agaaatggtc aataattgag ggtaggggtt agtatgtggt tgattaaaga ttgctcagta
     5641 atcaaccaca tttatatctt cttttgatta tttaataatc gcttttagtt caccttttgc
     5701 atatttcgtt gccatgttgt caagcatgat aggtttgatt ttacttgctt gaccggcaca
     5761 tccaaatgct tcaaaacgtg ccttacatat atctttagcc gcagccgtcg catcttttag
     5821 gaatttgcgt ggatcaaact cgctcttgtt tttagcaaga tggcggcgaa tagcacctgt
     5881 catcgccaaa cgaatatcag tatcgatatt gacttttctg accccatatt taatcccttc
     5941 ttgaatttct tcaacgggaa caccataggt ctctttcatt tctccaccat actcacgaat
     6001 gatttcaagc cattcttgag gaacagagct cgatccatgc attaccaaat gcgtatttgg
     6061 tatccgttta tgaatttctt taatccgttc aatcgcaaga atatcgccag ttggcttacg
     6121 tgtaaattta taagcgccat gtgaggttcc aattgcaata gcgagtgcgt ctacgccagt
     6181 cttcttaaca aaatcagctg cttgctctgg atcagttaat aactgatcat gagttaacac
     6241 cccttctgca ccgtggccat cctctgcttc acccattcct gattcgagtg aacctaggca
     6301 acccagttcc ccttcaactg atacaccgac tgcatgggcg atcgctacga ctcgagaagt
     6361 aacatcaaca ttatattcat aagaagaagg tgttttagca tcttcttcta atgatccgtc
     6421 catcatcacg ctggaaaaac cactgcgaat tgcattgaca cataccgctg aagaagcgcc
     6481 atggtcttga tgcatcacga taggaatatg aggataagct tctacagccg ccgcaatcaa
     6541 atggcgcaga aaagcttcac ctgcatattt tcttgcgcca gcagaacctt gcataattac
     6601 agggctatta cattcgtcag cagcctgcat aatcgcttga atttgttcta aattatttac
     6661 attaaatgct ggtaaaccat agccattttc tgcagcatga tccaacaatt gacgcaatga
     6721 aacaagtgcc atctatattc tccttcctaa atattaccta attaaaaaaa tatcaaaata
     6781 aaatcttaaa tgagtcttct cggtgattaa atcgctattg attgatcaga tattagcctc
     6841 aaaattatcg atattcagcg gcttcctaca atcgcccact cgaacaattt tcatcgtatt
     6901 ggtgccccca gctttaccaa caggttctcc aatcgtcatg ataattacat ctccatcacg
     6961 aacggcgcca cgcttaaata actcatcttc tgccagatca aggatcactt ctggatcata
     7021 taatccttca ccaaattcga caggatagac ccctctaaaa agcgtcattt tacccagcgt
     7081 atctttctgg ggactcaacg caaatatcgg gaccttcgag cttctccgcg acataaacaa
     7141 tgctgttttt ccactttgtg ttaacgcagc aatcgctcta attttgagat tacctgctgt
     7201 atacatcgtt gcacgcgcta ttgcctcttc tatactcaca ggaggcgtat cgggcgagcg
     7261 tcgtaaactt tgatttaccg tatattcttt ctctgcttcg agacaaactc tggccattgc
     7321 ttctactgat tctacaggaa actggcctgc agcagactca gcggatagca ttactgcatc
     7381 agttccgtca agaacggcat tggctacatc agacacttcc gccctggttg gaatcggatt
     7441 tgtaatcatc gattccatca tttgtgttgc agtaacaacc aatcgattat ttgcacgcgc
     7501 cgaacggatc atgcgcttct gcagtgcggg tactgctgca tcccctactt ctacggctag
     7561 atcaccacga gcgaccatta atgcatccga tgcttccatg atgtcatcca atgcaagaat
     7621 agcttcagtt cgttcaatct ttgccatgat taagctattt ccacctgctt cctgcatcaa
     7681 cgaacgtgct aggcgaatat cttcgccaga acggggaaag gatacggcta gataatcggc
     7741 tttaagatct gctgcaatct taatatcttc aatatcttta ctcgttagcg caggtgcgct
     7801 aagccccccg cctttgcgat tgatgccttt attgttcgaa agtggcccgc cttgtacgac
     7861 ttcacagaat atttcacttc ctttgactct gtttacgctt aaaacaatgc gcccatcgtc
     7921 caataatagg atcgcacctt cttcaacgtc attgggtaat tctttatagt cgagaccgat
     7981 tctctcttta tttcctagct gacagttagc atccaggata aattcgtcgc ccacattgag
     8041 ccagatttta ccttgttcaa attttcctat tcgaatttta gggccttgta agtcagctaa
     8101 aaccccgaca gtacaaccca cagaatgcgc taaggagcgt gtcaattcag caaaagcgat
     8161 atgttgctct ctcgtcccat gggagaaatt gattcttaca acatcaactc ctgccacaat
     8221 catgcgtttg agtacagctg gatcgctaca agctggaccc agtgtagcaa ctattttggt
     8281 tcttctaaac atgaccgagg cagcctaagt aaactattgc tttgctcttg actccagaat
     8341 ttctacggct ggaagtttct tcccttctag aaactctagg aaagcgcccc cggctgttga
     8401 aatatatgaa accttatcgt aaatatcgta tttttgaatt gcggcaatcg tgtctccgcc
     8461 gccagccagt gtaaatgctt gtgtttctgc aatggcttgc gcaattcgtc gagtaccacc
     8521 accaaattga tcgaactcaa atacaccaac tgggccattc caaacaactg tgcctgcttt
     8581 catgatgata tcaaccaact cttgcgcact attaggacca atatcaaaaa tcatgtcatc
     8641 atccgcaacg gtatcagccg atttagtgat agcaggctca tttgcatcaa attttttacc
     8701 aaccacgaca tcgaccgcaa tgggaatcgt tgcattccgt tgctgcattt tatccatcaa
     8761 taccttagct gtaggaacca agtcggcttc acacaatgat ttaccgatat tcttaccagc
     8821 cgccttcaaa aaggtatttg caatcccccc cccaacgacg agttgatcaa ctttttccga
     8881 gagtgattcc aatacagtaa gttttgttga aacttttgaa ccaccaacaa tagccaccat
     8941 cggccttgct ggcttatcca atgctttggt taacgcatca agctcttctg ttaataaaat
     9001 tccagcacat gcaaccggtg catatttagc aacgccatgt gttgatgctt cagcccggtg
     9061 tgccgtacca aacgcatcca ttacgaaaat atcgcacagg ctagcatatt tttgtgccgt
     9121 ttcatcgaca ttcttttttt cacccttatt gatccggcaa ttttctagta cgactaactc
     9181 gccctcagcc acatcaaacc cgccattcac ccagtcttta ataagtcgaa ccggtttgtt
     9241 aaggcgtgca gcgatattgt ctgcaactgg ttttagtgaa ttttcttcac tccatactcc
     9301 ctcttctggc cttcctagat gagaggtaac cattactttc cctccccgct ctaggcagtg
     9361 tttgatagtc gccatggatg ctgtaatcct ggcatccgat gttacctttc catctgcgat
     9421 gggaacattt aggtcagcac ggataaaaat ccgtttacct ttcaaatcaa gatcgacgag
     9481 tttaatgact gacacgttaa ctccattaaa actaattaga agattctggt cagcaaatca
     9541 atcattaact gaccagcttt gacgctttac ttgcttgcaa tgactttaac catctctaac
     9601 actttgcaag aataacccca ttcgttatcg taccaagcga ctactttcac gaagttttta
     9661 tccagagaaa tgcctgcttc agcatcaaaa acagaagtgc agctttcacc acgaaaatca
     9721 gttgaaacaa ctttttgatc ggtatatccc agtacgcctt tcatggtccc ctgagaagta
     9781 gatttcatta cgctacaaat ctcgtcataa gttgcgtctt tgttgagctc aaccgtcaaa
     9841 tcgacaaccg atacatcaga tgtcggtacg cgaaatgcca taccagtcaa ttttttattt
     9901 aattctggaa taacgacacc taccgctttt gcagcacctg ttgaagatgg aatgatattc
     9961 tcaagaatac cacggccacc acgccaatct ttgttagatg ggccatcaac tgttttctga
    10021 gtagcagtag cagcatgcac tgttgtcatc aaaccacgtt taatgcccca cgtatcattt
    10081 aatactttag caattggtgc taagcaattt gtcgtacaag atgcatttga aacaatggtt
    10141 tgacccgtat aggctttatc gttaacccca taaacaaaca tcggcgtatc atctttagaa
    10201 ggcgctgtca taattacttt ttttgctcca gctgtcagat gtttctcaca ggtttctttt
    10261 gttaagaaca aaccggttgc ttccacaacg atatctgcac ccacttcgtt ccatttaagt
    10321 tcagccggat ctttaatggc tgtgagacgt atgcgctttc cattgacgac caatgtattg
    10381 ccttcaattg caacggtgcc gctgaatcga ccatgaacag aatcatgcgt aagcatataa
    10441 gccaaataat ctggctccag caaatcatta attgcaacta catcaatatc gctaaatttt
    10501 tctatcgatg ctctaaaaac catacgaccg atacgaccaa aaccattgat tccaacttta
    10561 attgccataa tttttctact cctcaaatca attactaaaa taaaactagt acagcatctt
    10621 gagaaaactc atcgtgctta aattattctt tttattgcat ttacaacatt ctctgtcgta
    10681 acaccgaaat gcttaaacaa aacttcagcg ggcgcagatt caccaaacgt atcaatgcca
    10741 ataatttcac cttcaaggcc tacatattta cgccaataat cagttacacc cgcctcgacc
    10801 gcaacacgct tgattccttt aggcaagacc gcctcttgat acgccttgtc ttgtcgatca
    10861 aataaattag tacacggcat cgaaatcact cgtacatgaa ttccttcatc agcaagcgtt
    10921 ttttgtgctg ccattgcaag ccccacttca gatccagtcg caattattat tgctcgtggc
    10981 ttgccatccg ctgcttcaga taaaatataa ccacccttgt caattagctt gataacatca
    11041 gcctctcgtt tttggaaagg taaattttgg cgactaaaaa tcagcgttgt aggaccattc
    11101 ttccgttcaa tcgcacgtgc ccaggcggct gttgattcca ctgtgtcaca aggtctccaa
    11161 acatccatat ttggaattaa acgtaacgtt gctgtttgtt ctaccggttg atgcgttgga
    11221 ccatcctcac ctaacccaat tgaatcatga gtaaacacaa aaatactccg aattttcatt
    11281 aacgctgcca ttcgtaatgc atttcgtgca tattctgaaa acatgaggaa agtaccgcca
    11341 taaggaataa aaccaccatg cagcgacaat ccattcatga ttgcgctcat tccaaattcc
    11401 cttacaccat aataaatgta attaccgcct gattcgctac ttaccccttt tgaaccagac
    11461 cacaaggtaa ggttagagcc agctaaatct gctgatcccc ccagtaattc aggaagcgca
    11521 ggcgccaagc cttcaatggc gttttgggaa gctttacgtg tagcaatcgt ttcacctttt
    11581 gcattcactc ttcccactac accgtcaaca tacgctgacc aatctttggg taaatcacca
    11641 ttgacccggc gcgtaaactc agcagcctct ttaggatatt tctggctata ttcagaaaat
    11701 ttttgattcc actcactttc aagtttctta ccttttgtgc gtgcatccca tgcatcatag
    11761 acttctttag gcacttcaaa gggcagatgg tgccagccta tatgcggtct tgttgcagca
    11821 atctcttcat caccaagcgc cgctccatgc accgcatgcg tattggcttt attcggtgaa
    11881 cccatgccaa taaccgtttt gcaacaaatc atcgatggtt tatcttgttt cttcttagcc
    11941 tcttcgattg cggcttcaat tgcttctgga tcgtgaccat tcacattcgg gatcacatgc
    12001 caaccatagg cctcaaagcg cttaggtgta tcatcagtaa accagccttc aacatggcca
    12061 tcgatagaga ttccgttgtc gtcatagaaa caaatgagct tacctaaacc aagcgttcct
    12121 gctaatgaac aggcttcatg agagattcct tccatgagac agccatcacc taaaaataca
    12181 tacgtatagt gatttacaat atcgaaaccg ggtcgattga attcatttgc aagaattttt
    12241 tctgcgagtg ccatacccac cgcattggtg attccttgcc cgagtgggcc agtcgttgtt
    12301 tccacccctg gtgtataacc atactcagga tgtcctggtg ttttagaatg aagttgtctg
    12361 aaatttttta tatcttcaat tgacacatca tatccagtta agtggagcaa agcgtaaatc
    12421 agcattgagc catggccatt agacaataca aaacgatcac gatcagccca ttttggatta
    12481 ctaggattat ggcgcatatg atgaatccaa agcacctcag caatttctgc catacccatt
    12541 ggcatgcctg gatggccaga attagccttt tgtactgcat ccatcgccaa tgcacgaatt
    12601 gcgcttgtta gattcttgaa tactggcgct ttaaaatccc caaatgctcc catttatata
    12661 ctagctcccc aataatgatc aaaacgaaac cttccctgtc aaaaaattga caaggcgagt
    12721 cggcaaaaac tgttattatc tactaactta ggattcgcta caatatcgat tccacgatgt
    12781 gatgttgaaa cttaaccacc atgaccgcgt agtttaattc caagcatttt aatcttccga
    12841 taaagatgcg tccgttcaag acctgccctt tctgcaacac gtgtcatatt gccactttcc
    12901 tgatcaatga gcttctcaaa ataatttttc tcaaacaaat cccgcgcttc tcgcaacgaa
    12961 acattgagcg gtatatctgg tgtagcagac atggtgggcg ataatggggg cgtaagtact
    13021 tgttgaactt cctcagcgct aatttcactg ccagtacatg ttagcgccaa agaatgcaca
    13081 acactcttca actgtgccaa atttccaggc cagtcataat tacgcaatgt gttaagggct
    13141 gctgtactaa aatggacaaa aggaacttca cctgtttcaa tgctcgttga aagaagttga
    13201 ttggcaagct ctggaatatc ctcacgatgt gtccttaatg agggcacgtt aatcgttaga
    13261 ttactgagta tctcatacaa ttttgaattg taggtacctt gcgcagttag ctctgccaag
    13321 ggttgcgtcg ttgcacaaac caaccgaaca ttaaattttt ccaactttcc aagcaaaagt
    13381 agcaaacctt tctgttctag cttattaaga ccaccgatct ctctaaaaaa gagcagccca
    13441 tttttcactt gctctaaaat tcccacgggg ttatctgcca aaaaagaaag ttgctccggc
    13501 tcaacccagg gagtattaac gcgatgcaag aagcgagcac aatgctcaac ccctacaccg
    13561 gattcgccca ccaacaaaac cggcgacacc agattgacaa tttgctcgag tttcttcttc
    13621 aattcaacga tttgaggccc cttgcctaaa ttggccaaag aaatcgttaa cgctggctta
    13681 ctttgccctc cccgaagcgc ttgcttgact gtgctcaata atttctgaag gggaatcggt
    13741 ttttctaaat aatcaaccgc accgatgcgc gttgcttcaa cagcagtatc aatcgttcca
    13801 tgtcctgaca tcatgacaac tggcatagtc aatacaccac tatttgccca gtccttaagt
    13861 aaggtaatac catccgtatc gggcatccat atgtcgagta atactaaatc gggccgactc
    13921 tgattacgac aaacgcgcgc ctgctccgca ttttcagcca atactactcg gtagccttca
    13981 tcacttagaa tctcagacaa taattctcgt atgccaattt catcatcaac aactagaata
    14041 gtattgttgg ccatatatca ctcctttcgt atccgagttc aatctgcttc cttggtcccc
    14101 attatgcttt accactatca ggccaatcgg ttcattgtcg atgccacttg cccagcgtta
    14161 atcgccggca acgtaattgt aacttttgct ccatggggtt gggtattttc aatttggatt
    14221 gcaccattat gttcatcaac aattttttta acaattggca atccaagtcc tgtacctctt
    14281 ggcttagtgg ttacgtaagg ctcaaaagcc agtgttttaa tttcttcggc aaaacccttt
    14341 ccattatcga taatgcacaa cctaacattt cctgaaatca tatcagtact caccgtaatc
    14401 gtaccattct tagtatctgc tagcgcatct tgagcattct gtagtaagtt atgaatcact
    14461 tgtcgcaatc ttgccgggtc accccgaatt aacaaaagat cagaggataa cgctaatttt
    14521 atttctgggc actgcgaatt ctcggcttta gcattagagg tttcgtacaa agccaaaacc
    14581 tcatgaatta accgattaaa atcaatctga accatttcca attcaggaat acgcgcatat
    14641 tcactaaact catttaccat ctttttcaag gcttcaactt ggttgacaat ggtttcagtc
    14701 gaacgctgta aaattttagc atcctgtccg cttaatttat ccattaacct atgctgaatc
    14761 cgctctgcag aaagttggat cggggtcagt ggatttttta tttcgtgcgc cagtcgacgc
    14821 gcaatttccc cccaggccac ggtacgctgc acctgcaata atcctgtaat atcgtcaaac
    14881 acgacgattc caccaccgcc tgaagattca ggcaatcgcg tcccacgtag cagcaaaact
    14941 tggtctttac tttcactaaa atgcaaaacc tgcctttgcc attctccact ttgagtggac
    15001 ccaaatccat tctgaatttc tgatgctaaa agatgaaact taggctcttt ctcaacacac
    15061 tcatcgatcg ccaggccttc aagataaatt aatggaattt gtaagatttg ctccgcgctg
    15121 cgattaaccg ttcttatttt tagtttgtca tcaaatacca tcacgccaga cgaaagattc
    15181 gccagaatac tttctaaata agcacgcgca ctttcaatct catgctgact ttgttgcgca
    15241 ttaacgcgag ccagatctaa ttgctccgtc attaagttaa acgattccgt taaaataccc
    15301 agctcatcat tgctaaaaac ctgatgtcgc ctgctgaaat caccctgagc aacggcgcgt
    15361 gtcccttctg cgagcatacc cagcggcgca cttaattttt cactgataag agaagcgctc
    15421 gctagcgctg aaaaaagcgc taacagcagt gcaaatgtta atgtcacact atatagtcgc
    15481 gtaatcccct gttttgatag taccaactcc tggtaatttc gatacccggt ctgaacgatc
    15541 tcagcatttt cggcaaggcc agaaggcact tgctgcaaca cttgcaatac gcgaatatcg
    15601 tcttccagac tcagcacatt aacaggaacg agaacccgta aaaacaaccc tttattagga
    15661 attgactcaa ctgcgctata gctcttttgc attctaatat gtcgcatagc agttgcatta
    15721 ggtatctctg gaaataacgc gacatcatca agacttgaaa acgcaatggc ttttccatct
    15781 tgattgaaca aagtcgcttc ttgtatatag gaatgtgcta aaagttggtt taatgaagaa
    15841 agagtttgag acggcgaatc agcaagcgct agtgctgtaa ccttcgcttt tttttccagc
    15901 tcttccagta aattatccag catggtttgg cctagattga ttccaccttc aagcgcttga
    15961 tcaactttga catcgaacca agattcgata ctcttcccga gaaattgaac tgagatcccg
    16021 tataccaatg ccccagggag aattgccatt aatgaaaaaa ccactaacag tcgaaacgcc
    16081 aaccgcgaac caaaaactcg tgttttaagc ctttttctta gcctccatag taagaagcca
    16141 acgataccga ggagcagcaa cacaaaaaaa acattcagta caatcagcca acgataaaat
    16201 ttccctagaa aatcggtatt cgcccctgcc gttgccaaga gaaacagcat gacgacactt
    16261 aatcctgtaa cgagaaggat aagatatttc actgattatc tttgaattga aaatgatggc
    16321 gctgcaggca gtttagtgcg ccattctttt ttttctgaac tcaagttcca ttctttggaa
    16381 ctcaatgatt caagttggaa gggcttagat aagcgagaag catcaagcca tacttgtaag
    16441 ctagctaagt attccatgtt gggcttaagc tccgatttct cttcaaccgg aatataatgc
    16501 tgagtgccca gctcttgaag tgcatcgatt aatgtatcga acccttgcga tttggatcgg
    16561 tggctcagcc gatactggcg ggttagtgca ttataactta ggccgattct taatttactt
    16621 cttgctacct catcatccac ccagtaccac ttggtatcaa tcaatgcaaa tttaatcaca
    16681 aaatataaaa caatcccctt ttcgagggcc tgctctaagg tactatttaa ttcaattgcc
    16741 gcatcaacat taatctgata accagcatcg gttgcctcca atgcaaatga tttaattttt
    16801 atgttgcctt cactatgggc cgactgaatg gttacaccaa tgagtacccc ccctaatatc
    16861 agcatactaa ccagtctttc caaccaattc atgccgttat attttataaa atgaggcata
    16921 ataaaaacca tcatgagagg agttaggtat caattgccca ttattaactt cattatgtac
    16981 gattggaagt aagcgagcat caggatgttt atccaagaaa ttcgttactt gctgaccgtt
    17041 ctcttcaaaa aaaacagaac aagtcacata gagcaatttc ccttcacttt taagaagtgg
    17101 ccacaatgca tctaaaatat tttgttgcat attcgcaaac gtagcaagat cggtgatacg
    17161 ccgcagccac tttatatcag ggtgtcggcc aactactccg gaagcactac aagggacgtc
    17221 tgccagaatt cgatcaaagc atttcccgtc ccaccatgaa gatggtaatg ccgcatcccc
    17281 acagacttgc tgaaagggtt caattcctaa tcgcttaaaa ttttctgaca cgcgacccaa
    17341 acgctgttgg tcattatcga gaaccgtcaa ttggatattt gccacttcca ttaaatgagt
    17401 gcttttcccc ccgggagctg cacatgcatc aaggacagtc attccatcgc gaatatccag
    17461 aaaaggcgca gctaattgag cccccgcatc ctggatcgaa acatagcctt gctcaaacat
    17521 cggtaatttt tgtgtgggta ctggcttaga taacataagt gcattatccc atacttggca
    17581 gctttcaatc tcacaccgat taagtagcgc ttgataatcg atgacgctgg ttttgtgtcg
    17641 attcactcgc aacgtcatgg gaggatgatg attattcgcc agcaaaatcg cttcatactg
    17701 ctgtgggtat tgatatctta atttatctat ccaccattgt ggatgcgaaa atcttcctgt
    17761 ttcatgtgca ttggctagat ccaacaaagc cgtatcattt cttaaaaaat ttcttagaat
    17821 cgcattaacc aaacctgctg aacctttgtt tttagttaat ttccgagtcg ttgcgacagc
    17881 acaatctaca attgcgtagt caggtgattt actataatga agctgatata agcttaccag
    17941 cagtagattt cgcaaccgtt tgtctgacaa gggtcgcttt actaattggc tcaacacccc
    18001 ttccagttgg cctaaatatc gcagcacacc ataaactata tcttgaatag cgccgcgctg
    18061 ctgcaatgat agtaacggat tatgatgcca gagcgcttgc agcaccaagg ttaaattttt
    18121 cccagacaaa actatttcga ttgaatctac agcaagctgc tgaattttca tcataccaat
    18181 atcagctatg ctactccctc ctaaatgccc taattctagt taaactacag aatgggttaa
    18241 tcagtgaccg aagcacgact aaaccatgaa ctcaccccgt ttcatgggat tccctttaag
    18301 aaaatccttt actggtaatt ttctcccccc ggctctttga atcacttcca gaactatcgc
    18361 gtgcttacca caagcaacgg taataccgtc attatcgata ttaacaatct caccgggctg
    18421 cccagaaagc ttctgttcaa ttcgtgcttg ccatattttc aacgatagcg attgatagct
    18481 agtaaaagca acgggaaacg gattaaatgc gcgaatattg cgatcgatca cggctgcatc
    18541 aagactccaa tcgatttttg cttcatcctt ttcaatttta cgcgcataac aaacttcctc
    18601 atcattctgc tcaacggcca acaattttcc ttgttgtaat agaccaagcg cttcaataat
    18661 acattgcgca cctaattgcg ctaggttgtc gtgcaatgat tgcgccgtat cggatggatc
    18721 gattggaatg gttctctgca ataatatgtc acccgtatct aaaccctgat ccatttgcat
    18781 gatcgttatc cctgtttctt gatcaccagc ttgaatggcg cgctggatcg gggcagcacc
    18841 cctccacctc ggcagaagag atgcgtggat atttaaacat ccctgtaacg gtaactgcaa
    18901 aattgattct ggcaagataa gtccataggc agcaacgatg ataacatctg ccgcaaaaga
    18961 ctttagctgc tcttgcgtta cctctgattt taaagtaacc ggttgtaaaa ctggaattga
    19021 atattcactt gcaactttct tgacttcact ttcttgcagt ttcaatcctc ggccagcggg
    19081 tcgatcaggt tgcgttagaa cgagcacaat ttgaaattgc gcatcgatga gtgcctttaa
    19141 tgcgattgca gcaaaagcag gggttcctgc aaaaactatc ctcataaaaa tcgaacccga
    19201 tgtatcatca aacgctcatc gataacaccg aaggtttggt aaggcgctgc tgtttcttta
    19261 atttggtctg gattcttgat tgctttaatt gcgacaagta ctcaacaaaa accttaccct
    19321 ccaaatgatc gatttcatgt tgtatacaca ccgcgagcaa accttctgct tgcatctcga
    19381 aggattcccc ctggagattt agcgccctga cggtaaccag ctcagcacgt ttaaccttat
    19441 caaatatgcc aggtaacgat aaacaccctt cttgaaactc agcgtcaccc gccttagtta
    19501 ttatttctgg gttaataaat acattgagtt ggtcatgtgt ttcggaagta tcgatcacaa
    19561 ttacacgttg atgaacatta acttgggtcg cagccaaacc aattcctgat gcgtgataca
    19621 tcgtctctgc catattttca actaacacac gagtatctcg agtaattgct ggcacaactg
    19681 acgcaatctt atgcaatcgc tcatctgggt actgtaatat tttcagaatt gccataacaa
    19741 atatgcgtaa ttcacgctac tgaataccga agttaaatcg taaaatcgcg gcgatgataa
    19801 tcgaatttaa atccattttg ttttgtcccc actacactct tggaaaaaat aaatttatgc
    19861 gtactatttc cattatagcg atgattttag ctatcagtat attttctgac cgggctgcaa
    19921 aagcaaaaga tatcgaatta ctcgccaatg cgcctgaacg atatgttgta tctcaagggg
    19981 atacgctttg gggaattgcc tctcgctatc ttaaagatcc ctggcaatgg acagaaattt
    20041 gggacatgaa ccaaaaacaa atcaagaacc caaataacat ctaccctggt gatattatta
    20101 ttctagtccg agctgcggat ggaagcagta aactaatgtt tgccaatgaa aaagcaacga
    20161 tcaaactctc acccaaaatg cgcattgaga aacttgaggc tgagaaaatt ccatcgattc
    20221 catcaaaaaa actcaagccg ttcttaagtc aacctttagt cattgaaaaa aatggattgg
    20281 atcgggcgcc attaatatta ggctccagcg acaatcgcgt tatattaagc actggtaaca
    20341 cgatctatgt acaaaatatg ccttttgatc aaggcatggc ttggcaagtt ttccgtgcag
    20401 gaaaaccact cattgatccg gaagacaacg atcgcgtcct tggccatgag gcagtatatc
    20461 taggcgatgt agaggtctca aattttgatg cgatcagctc agttagaatt acccattcgg
    20521 tgcaggaaat tctcaagggc gaccgcctga ttcctgcttc tacactacat gttgacgatt
    20581 acattccccg tgcacctgat tttccggtca caggacgaat catttcagtt tatggcggtg
    20641 taactgaaat cggtcaaaat gcgattatcg cccttaataa aggatcatcg gatggattag
    20701 aagtaggtca tgtgcttgct gtttatcgta aaagcaccgc caaattgcaa gacagcaaaa
    20761 tcatctcgtt accggatgaa cgcattggat tagtttttct atttcgagtt tttgataaag
    20821 tatcgtatgc tcttgttttg catagcgctc aggcgattaa agtatttgat gccgtgcaaa
    20881 caccataaaa aaaacaattt aataaaaaca atttgattgg ttatgaatat tcagccagat
    20941 attgaatcct ggctaaccct cagcttcatt gaaggacttg gtgatgaatc cttccgtaag
    21001 ctgcttttaa cttttaaaca tcctacggac atcctcaatg ccgaggctca acttctcgag
    21061 cgctatgtaa aaaagccaat tgccgcagat atcgccaatc gtcgcgttga tcaaaagaaa
    21121 ataatcgcta cactgaagtg gcttgaggat cctgctaatg caatcattac attaaatgat
    21181 gacaattatc ccaagcgatt gctcaatatt gccgaccctc ctccactgct ctatctaaaa
    21241 ggtcagcgcg aactactcaa tactccctcc attgcaatcg tcggaagccg taacggtacg
    21301 cctcaaggca ttgtcaatgc gagtgcattt gctgaagccg caagcagtgc tggcctttgc
    21361 gtcgtaagcg gtatggcatt gggtattgat acagctgctc accagggcgc tctgctcggt
    21421 tcttccagta gtattgctgt tgtgggaacc ggattggata tcgtttatcc agccaagaat
    21481 catcagctag cccatcaatt agccaaagaa ggcgctctca tttctgaatt tcccttgggt
    21541 atgccagcaa tcggaagaaa ttttcctcgt agaaaccgaa tcatcagtgg catcagcttg
    21601 ggttgtctcg tcatcgaagc gactttgaaa agtggctcgc tcatcacagc tcgacaagcg
    21661 cttgaacaag gacgtgaagt cctggcagtt ccaggatcaa ttcattcacc tttatcgaaa
    21721 ggttgccatg cgttgattaa gcaaggtgca aaacttgtag aaaatattaa tgacattctc
    21781 gatgagttta attatcaacc gcttaacttg gctaatccgt tagcaactga tgaggcgatc
    21841 aaacaaactt acgctcaaga aaacacgatt ttactttctc atttagatta tgaagcaact
    21901 gatatcgaca aattgtgtat tcggagtggc ttgactgtgg aaacagtatc cgccatgcta
    21961 ttagctctgg aattagatgg gattgtggct agtttaccag ggggtcgata tcaacgaatt
    22021 cgttgattaa caatacaatt cattaaaatt ctaatgcgaa atataatcat gaagagcgaa
    22081 gcgatgttcg agattttaat ttacttattc gaaaattatt ttgattctga aaactgccca
    22141 gattcagaga cgttaacacg gaaattaaca ctggcaggat ttggagaaga tgagattagc
    22201 cagattcttg gctggttaac ggatcatacc aagcatgaag caggaaagta cccggtaact
    22261 ttatctcaaa gtaaatcgtt tcgaatttat tcttcttctg agttagaagt cattgacgtt
    22321 gaaagtcaag ggtttattct ttttcttgaa caagccggtg tgctcaattc attacaacgt
    22381 gagctgttaa ttgatcgtat tcttcagatg gatggcagta gttcctgctt ggataaaatc
    22441 aaattagtcg tcatgttcga tctatgggta caaaaccagc taacgaatca cgatttaatc
    22501 gaaaaattat ttattgccag caacccacgc aatcgacact aaagttgact catgatgagt
    22561 ctctaatgcc atgaaaaaac ttaaaaaaat aacctcatcg atctgatcac ccttcaataa
    22621 ccttggttac tcaatctggc cagcatcaga tgaagtacta acataaccca atcacctaaa
    22681 aaaatatcta ataaagtaat aacagaattt atgagtaaaa agctgattat cgctgagaag
    22741 ccttctgttg caaacgatat tgccaaggtt ctaggaaatt ttacgaaaga agctgattat
    22801 ttcgagaatg atgagtacat tctatcctct gctgtcggac acttgctcga attagcggtc
    22861 cctgaagaat atgaagtcaa acgcggcaaa tggagctttg atcacttacc ggtcataccc
    22921 cctcattttg atctaatccc tttagaaaaa acagcaacac gcttaaaaac actaacaaaa
    22981 ctcatcaagc gaaaagatgt cgacacgtta atcaatgctt gtgacgcggg ccgagagggt
    23041 gaactgatat ttcgctatat tgtgcgccat agtaataccc aaaagcccat caaacgcttg
    23101 tggcttcaat ccatgacgcc atccgctatc aaagaagggt ttaataatct actcaatgat
    23161 agtgatctac agcccttggc cgaggctgca gtcagtcgct ctgaatcaga ctggttggtt
    23221 ggaatcaatg gcactcgtgc catgaccgcc tttaattccc aagaaggggg atttcataaa
    23281 acgacggtcg gccgagttca gacacctacg ctcgcaatct tagtcgagcg agaagaagcc
    23341 atcaaaaaat tcatcccgaa aaattactgg gagctccatg ccaccttcag tacaaacagc
    23401 cattcttata aaggcaaatg gtttgacgaa aaatttacta aaagtaaaga aaatgctgag
    23461 gcacgcgcag aacgtatctg ggatttagca aatgcagaag cgatcaggct aaaatgtctc
    23521 ggtaaatcag gcactgtcag cgaagaaagc aaaccagcca aagaaacttg cccgctactt
    23581 tatgatttaa ctagcctgca aagagaagcc aatagccgct ttggattttc agcaaaatca
    23641 accctcgggc ttgcccaagc gctttatgag aaacataaag ttttaaccta tcccagaacg
    23701 gattcacgcg cgctaccgga agattatctt gaaaccgtca aagaaaccct acagtcttta
    23761 acagcgagtc cctatcacga attcgccagt caaattcttg tttctgattg ggtgaaaccc
    23821 aataagcgca tttttaacaa cgctaaaatt tcagatcact ttgcgatcat tccgacttca
    23881 ctcgagccca aaaaactaaa cgaagcggaa ttgaagctct atgatttagt caccaagcgt
    23941 tttcttgcga ttttttatcc ctccgctgaa tatctcgtta ccacaagaat cactcgtgtt
    24001 gaaaatgaaa ccttcaagac ggaaggtaaa atcctggtca atccaggatg gcaaatcgtc
    24061 tatggcaaat caagccctag cgctgatagc gatcaagaaa atttaacttt agcggcaatt
    24121 ggccctaacg caactgtcaa aacagaagaa atcgatacca ttgccaatca aacccgcccg
    24181 cccgcaagat tcaatgaatc aacgctacta tcggccatgg aaggcgctgg aaaattagtt
    24241 gaagatgaag aactcagagc tgcgatgagc tcgaaaggac taggcacacc agcaacacgc
    24301 gctgcaatta ttgaaggctt agtctctgag cattatttgc tgcgtgaagg aagagagtta
    24361 ctccccacgg ccaaagcatt ttcattgatc acattattac gcggattaaa aattcctgaa
    24421 ctgatctcgc ctgaattgac aggagattgg gaatataaac ttcggcaaat tgagcaaggt
    24481 caattaaagc ggcaagattt tatgaataaa atcgctgaga tgaccaagca tatggttgat
    24541 caagcaaaga gtcaccgtgg agaaaccatt agtggtgact ttgcagtgct taaaacaccg
    24601 tgccccaaat gcggcggtat tttgcatgaa acctataaaa aatttcaatg ccagcaatgc
    24661 gattttgcgt tatggaaaat attggcggga cgccagtttg aagtcgacga aatggagaca
    24721 ttgatctcgc aacgggaagt aggtccttta ctcggcttta gaagcaaaat gggacgttta
    24781 ttcactgcca gcatcaaact cactgatacc tttgaaatga agtttgaatt tggcaatggt
    24841 gacgatttag aagcagaacc aattgatttc tccaatcatc aattactggg aacatgccct
    24901 caatgcaatc atcacgtcta tgaacatagc accaactacg tctgtgagaa ggcagttggc
    24961 cctagcccaa cctgcaattt caaagtcggg aaagtcattc tcaatcgtgt gattgagcaa
    25021 gaacagataa tgaaattact tacacataaa aaaacagatt tattaacgaa tttcatctcc
    25081 aaaaaaggcc gacctttctc tgcctatctt gtagttaacg ctgataacaa aattggtttt
    25141 gaattcgagg aaagaaaacc atcaaaagta cgaagtaaga aaacaagcaa gaattgaatt
    25201 tctcagaatg aatacctaac cctcacacta tttctttaag ttatacttag ttaggaaatt
    25261 caatcgtaaa cagttcattc aataacgcga ccaagcgatt tgattcaaaa tgttccgtga
    25321 tcgtaatgat gcagcaaacc aattagctaa acaactcgat cattatcgcg atcttcgccc
    25381 gctcatttta gcgattccac gcggtgccgt tccgatggga aaaatgattg ctgatatgct
    25441 ctcaggtgat ttggacgtag ttcttgtcag gaagttgcga gcaccaggta accccgagtt
    25501 tgctatcggt tccattgatg agagcggttg gacctatcta gcagattacg cgggacaatt
    25561 tgtgcacgat tctcagtata ttgaagcgga aaaagctgct cagttaaaaa cgatgcaagc
    25621 gcaacgcaag caatataccc caattcgccc gccattcgat ccagaaaatc ggatcgtaat
    25681 tgtgatcgat gatggtttgg ctacaggatc caccatgatt gcagcattac atacattaaa
    25741 ggccaaacat ccaaaaaaac taatctgtgc tgtacccgtc gcaccacccg ataccttaga
    25801 aaaaataaaa ccctacgctg acgaaatcgt ttgtctcaat tctccgcgtg atttctacgc
    25861 agttggtcag ttttatcaag actttccgca ggtcagcgac gaagaggtca ttcaatcctt
    25921 aaagctcagt cgccattaat ctcatgctgc ggtatcggtt aaataatga
//
LOCUS       DAOQLJ010000014        25278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_643_length_25278_cov_5.808310, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000014 DAOQLJ010000000
VERSION     DAOQLJ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 25278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25278
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_643_length_25278_cov_5.808310"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1100
                     /locus_tag="PK522_02095"
     CDS             165..1100
                     /locus_tag="PK522_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNC40556.1"
                     /translation="MDTLEQQLSERLGRIHARQRLGIESDHKQQVFGKGLNFFHPENW
                     YATHTLIRFAIQLVGLYGRGQRNARNLQVRENSFYLAHLPEAFHQFRILHLTDLHVDM
                     DDRNLTALIETIIDLPYDICVLTGDYRAQTFGTIDHAIAGMQRLIAMLKQPIYGVLGN
                     HDSIRMLPALESMGVIMLMNEAVKLEKDGESIFLAGVDDPHYFRVDNLEKAAQTIPHD
                     AVSLLLSHTPEIYRQAAHADFDMMLCGHTHGGQICLPGGIPVTLDSSCPRYLGAGKWQ
                     YRTMQGYTSVGAGTSIVNARFNCPPEVTIHQLLKN"
     gene            complement(1097..1576)
                     /locus_tag="PK522_02100"
     CDS             complement(1097..1576)
                     /locus_tag="PK522_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HNC40557.1"
                     /translation="MVEITLLIWLICVNGAPILLAKILGKRFNIPIDGNRLWRDGQPL
                     FGQSKTIRGILVALLVSIGAGSLLQLPIVMSITFGLLSMVGDLTSSFVKRRLGYKPSA
                     MALGIDQIPESLFPLLGIQFWLTLSFSQIVGIVLTFFCVELMLSRILFWLRIRKTPY"
     gene            1760..2155
                     /locus_tag="PK522_02105"
     CDS             1760..2155
                     /locus_tag="PK522_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40558.1"
                     /translation="MKIALFKVSTIAATTLAVMALSLPASSAEAADIRVQCEKRSNRS
                     KVSVDGNNLISGNYSAKITSGANTKVSAYKPSVGDEIEFDFDSDRNDVAAGAIRISSS
                     FIQNGQVTGELINEKGFTVASSTSACRIK"
     gene            complement(2222..3385)
                     /gene="dinB"
                     /locus_tag="PK522_02110"
     CDS             complement(2222..3385)
                     /gene="dinB"
                     /locus_tag="PK522_02110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNC40559.1"
                     /translation="MNQSSRRIAHLDMDAFYASVELLRYPQLKGLPVVIAGRAEHKPK
                     IQADGQLQFSRLSDYIGRGVITTSTYEARALGIYSAMGVMKAAQLAPQAILLPVDFTA
                     YRHYSRLFKAAVVEIAPEIEDSGIDEIYIDLTTLADDNHTLAKRIKQAVLNATGLTCS
                     IAITPNKLLAKIGSDLNKPDGVTILSAEDIPTRIWPLPARKINGIGPKAEKKLIALGI
                     TTIGQLAEAELHWLQIHFSHNYAIWLYEAARGIDDRPVLTCSEPKSVSRETTFERDLH
                     PIRDRSILTAELIALCKRVANDLNRKAYQGRTIGIKIRFEDFNTITRDITLGKSVIDA
                     TAILSATRNCLRRVKLDKKIRLLGVRVSSLVALAEMQPLEDLHQVELPLSSNR"
     gene            3579..4190
                     /locus_tag="PK522_02115"
     CDS             3579..4190
                     /locus_tag="PK522_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40560.1"
                     /translation="MFTKIFKGLGLSLLVTGVLVGCTTTQKITSSARTTTEQLLISEA
                     LKRSLPKQTNAPLPIPQGSVVKLDVIGLTGDKDIVKGAVAGWLGQHGYIAQDEPATYR
                     MNIMVDSIGTEQANTFFGIPPISAALIPISLPKLAFYEAEYQTGYSRIHLDIFEVPSG
                     RFVGSTSSFIGDTYFNAHTILFLFKLKRTDLTSPPEVGTFMGH"
     gene            complement(4271..4831)
                     /gene="pgsA"
                     /locus_tag="PK522_02120"
     CDS             complement(4271..4831)
                     /gene="pgsA"
                     /locus_tag="PK522_02120"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNC40561.1"
                     /translation="MPFNLPNLLTWLRIFAIPLFVGIFYIPGLSPFGQNLVATIIFSV
                     AAITDWLDGYLARTLNQTSAFGAFLDPVADKLMVAAALIILLHLERLDAPIALIIIGR
                     EITVSALREWMAQIGKAKSVAVSFMGKIKTTAQMIAIPLLLYHETIGESFNSHLVGTW
                     FIYLAAFLTLWSMIYYLKAAIPHALK"
     gene            complement(4905..6722)
                     /gene="uvrC"
                     /locus_tag="PK522_02125"
     CDS             complement(4905..6722)
                     /gene="uvrC"
                     /locus_tag="PK522_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNC40562.1"
                     /translation="MSEVAFDGKVFAAALPNLPGVYRMLNAAGEVIYVGKAIDLRKRV
                     SSYFQKSMLGPRIQLMVSQITGIETTVTRSEAEALLLENNLIKSLNPRYNILFRDDKS
                     YPYVKLSQHPFPRLAFYRGTIDHRHQYFGPFPNAGVVRESIQLLQKVFRLRTCEDSVF
                     HHRTRPCLLYQIKRCSGPCVNLIEQSDYQADVKSASLFLQGKQNEVIRNIADKMQQAS
                     NQMDYEQAAVFRDQMQALRKIQEKQFVDSGKSLDADVIAGVKASDGSEKVCVNLVMIR
                     GGLHLGDKSFFPQNIQGYEMVDVLEAFIAQHYLNRSIPPIIIIDTKIQTKILQSLLTE
                     QANRKVTIISNSIGERRMWLDMATANADLALKQQLQRQASQEDRLQILQQALNLSNLN
                     RIECFDISHTAGEATVASCVVYDHFAMQNNEYRRYNITGVEPGDDYAAMKQALHRRYA
                     KVAEGQGKIPDLILIDGGKGQVSVAQEILTELGLADIPFLGVAKGEDRKPGLEQLVFP
                     SGEKPLKLPADHPGLHLIQQIRDEAHRFAIQGHRARRNKARTVSTLEEIAGIGAKRRQ
                     SLLGRFGGLKGVLTASVEELQQTEGISQALAERIYRELH"
     gene            complement(6742..6927)
                     /locus_tag="PK522_02130"
     CDS             complement(6742..6927)
                     /locus_tag="PK522_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HNC40563.1"
                     /translation="MPYVNIRVAGTLTREQKQKIAEEITDTLERIGNKPKSYTYISFD
                     EMPHDTWAIAGKLLGDD"
     gene            complement(6944..7597)
                     /locus_tag="PK522_02135"
     CDS             complement(6944..7597)
                     /locus_tag="PK522_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNC40564.1"
                     /translation="MGIDSSLVHCRRCSRLASFLDAVKAQYPTYHARPVAAFGDINPK
                     LLIVGLAPGMHGANRTGRPFTGDYAGILLYQTLFKFGLANQNESISSTDALCLLHTRI
                     TNAVKCLPPENKPKPCEIKQCNDYLSNEIIHFIEQGGKGILALGNVAHQAVLMAFNLK
                     LKRYPFSHGAIYVLPNELKLYDSYHCSRYNTQTKRLTQEMFEQVFSEICADLAIPNH"
     gene            7924..9462
                     /gene="murJ"
                     /locus_tag="PK522_02140"
     CDS             7924..9462
                     /gene="murJ"
                     /locus_tag="PK522_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNC40565.1"
                     /translation="MNLLKALATVSSMTLISRILGFVRDVLIARIFGAGVATDAFFVA
                     FRIPNLLRRLFAEGAFSQAFVPILAEYKNNRSEAETRALIDHVTTLLGVTLFVVTLIG
                     IVAAPLIIYISAPGFSGDIEKFSLTVTLLRITFPYILFISLVALAGGILNTYSQFSVP
                     AFTPVLLNLSFIGCSLWVVPYVDPPVLALAWAVFLGGILQLLFQIPYLIRIKRMPRIR
                     FKTKDTGAGRVLKLMLPAIFGVSIGQISLLINTIFASLLITGSVSWLYYADRLMEFPA
                     GLLGVALGTVLLPSLSRHYASHSNEEYSRLLDWGLRLTVLLTLPAAVALALLATPLIT
                     TLFYYGAFTAEDVWMTRQALIAYSFGLLGLIMVKVLAPGFYARQNIKTPVKIAVLTLA
                     ATQVMNLIFIIPLQHAGLALAIGLGACLNAGLLYYKLRTNDIYQPLPGWLKFMVKILV
                     AVIVMGTTLWLSSGNDSLWLQMNAIDRALRLTFTVCIGAIAYFITLWLLGFRLKDFSQ
                     LGTS"
     gene            complement(9472..10281)
                     /gene="folE2"
                     /locus_tag="PK522_02145"
     CDS             complement(9472..10281)
                     /gene="folE2"
                     /locus_tag="PK522_02145"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNC40566.1"
                     /translation="MNKQIDMPIADVQSSLDTRQIAIDRVGIKAIRHPVRVADKGGGV
                     QHTVAMFNMYVNLPHNFKGTHMSRFVEILNSHEREISVESFEDILRAMVTRLESDSGH
                     IEMTFPYFVNKSAPISGVQSLLDYEVTFIGEVKQGEYRFTMKVVVPVTSLCPCSKKIS
                     DYGAHNQRSHVTVAVRTNSFVWIEDVIKIAEEQASCELYGLLKRPDEKYVTERAYDNP
                     KFVEDIVRDVAEVLNHDDRIDAYVVESENFESIHNHSAYALIERDKHRKQI"
     gene            complement(10441..12285)
                     /gene="dxs"
                     /locus_tag="PK522_02150"
     CDS             complement(10441..12285)
                     /gene="dxs"
                     /locus_tag="PK522_02150"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNC40567.1"
                     /translation="MYPLLDRINSPIELRKLDRKQLPQLADELRQFLIESVASTGGHL
                     SSNLGTIELTIALHYIFNTPYDRVVWDVGHQTYAHKVLTGRRAGMAKLRMKGGVAGFP
                     RRDESEYDAFGTAHSSTSISAALGMAVASQLDDATRRAIAIIGDGAMSAGMAFEALNN
                     AGVMDANLLVILNDNDMSISPPVGALNNYLAKLMSGRFYATARRAGEKVLGVVPPVLE
                     LARRAEEHVKGMMTPGTLFEEFGFNYIGPIDGHDLDVLLTTLSNIKHLDGPQFLHVVT
                     RKGAGYKLAEEDPILYHGVGKFDPHKGIIAKAASKPTYTQVFGDWLCDMAALDTRLIG
                     VTPAMREGSGLVKFSQEYPNRYFDVGIAEQHAVTFAAGAACDGLKPVVAIYSTFLQRA
                     YDQLIHDVAIQNLPVVFAIDRAGLVGADGPTHAGSFDLSYLRCIPNITVMVPADENEC
                     RQMLYTAFQLNTPSAVRYPRGSGIGVKIQKKMNALPIGRGEIRRSGSKIAILAFGTLL
                     TPCLEVGEVLDATVANMRFVKPLDDELVSDLAKTHDLLVTVEENTIMGGAGSAILESL
                     MASSVTVNVLQLGLPDVFLDQGDPVQMLADCGLDKAGILKSIQDRLAK"
     gene            complement(12338..13228)
                     /locus_tag="PK522_02155"
     CDS             complement(12338..13228)
                     /locus_tag="PK522_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNC40568.1"
                     /translation="MSVNFQEWVKNCQHQLELFLEERLPNNRTAPERLHDAMRYAVLG
                     GGKRVRPLLAFAAGELGGADISRVRVVAAAVELIHAYSLVHDDLPCMDDDVLRRGKPT
                     CHIQYDEATALLVGDSLQSLAFQLVAEYPVTDNPTDQLEMIKQLAQASGSRGMAGGQA
                     IDLASVGKSITLPELESMHIHKTGALIRAAVMLGAHCGNQLSSHDLSRLDHFAKCIGL
                     AFQVVDDILDSEATTATLGKTAGKDAQQNKPTYVSILGVKQARELAESLQQDAYKALS
                     EIKSTQRLLQVTDFIIQRHF"
     gene            complement(13218..13475)
                     /locus_tag="PK522_02160"
     CDS             complement(13218..13475)
                     /locus_tag="PK522_02160"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNC40569.1"
                     /translation="MKKKTTETLIVEPESFEAASAELESIVIKMESGQMTLEASLAAY
                     KRGMELLQYCQKALQNAQQQIRVLEDGNLRDFTETESINER"
     gene            13926..14405
                     /locus_tag="PK522_02165"
     CDS             13926..14405
                     /locus_tag="PK522_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40570.1"
                     /translation="MENSLDQIIKYFEEVPLWPFVLLAGLLLFSLGIEFVNRRRRIDA
                     VEYFDSTFLTELAGLYPQATRWPEDLAEHLQESFPVMRDAFENLRGFLPQKQLREYNA
                     AWNNFFDFCRTNGQSHRNVNVKSVSDQPSELNQSAQDIETFHKLVSALLAYSNQFKR"
     gene            14522..15655
                     /locus_tag="PK522_02170"
     CDS             14522..15655
                     /locus_tag="PK522_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNC40571.1"
                     /translation="MSEIVVRPVDTYQRMGHFIEVPWLVYKDDPNWVPPLRLERRFHY
                     SRFNPFFEHAEWQAWVAYKDNHAVGRISAQIDSLHRQHYGQQTGHFGTLECINDEAVF
                     ATLINSAEQWLIEHDAFNITGPFNLSINQECGVLVEGFDTPPVVMMPHSRKWYDSLLQ
                     QYGYQSARDLLAYWVDVDFTAPKAMQAVNQRYRDQIKLRTLRRKHFNEEMETLRDIFN
                     DAWSENWGFVPFTKAEFSELGNSLRWLVPEDYIQIAELEGRPVAFMAGIPNLNEIFSQ
                     LNGSLFPFGWIRLIKLLKSRAIKTGRIPLMGVRKQYQKTPIGLALAFAVIDAPRQIGI
                     RQGIKAVEMSWILEDNSPMRSILKSIGSKEYKRYRLYEKTLVA"
     gene            15662..16468
                     /locus_tag="PK522_02175"
     CDS             15662..16468
                     /locus_tag="PK522_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNC40572.1"
                     /translation="MNSNLNYAALVLAADRTANDPIALKTQVPCKAFAPIDGKPMIIR
                     VLDTLIASPTVKSITLCGPPQALLPECPELHERILHGQIGWITNQNSPSLSAAKGLEQ
                     LDPDIPVLLTTADHALLTPEIVEYFLKSASDNEYDATVGAINYEIIHAAFPESKRTVI
                     RLQDGKYCGCNLFAFKPRGRLLVSYWQKVEAQRKRPWKLISQTIGWQAVFKYLLGFLT
                     LNQALSHIEKQIGLSVKIATLPFPEAGVDVDTVSDLMFVEALLKKKAENT"
     gene            16502..17578
                     /gene="lptF"
                     /locus_tag="PK522_02180"
     CDS             16502..17578
                     /gene="lptF"
                     /locus_tag="PK522_02180"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HNC40573.1"
                     /translation="MKIIEKYITRELLIPFFVVIGILIGLFSSFSIARFLTSAVTETL
                     GMIAMLKLVSLRTIIALEVLVPIAFYVAIIYGLSRLNKDQELNIIRSSGFGDNRILFT
                     ILVIALPISIISGVLSLYARPWAYAESYILDAQAEAELNADRFQAGRFYGSEKSGRVI
                     FVQSKDDTGQHMENVFHFVRKGENSEIIVAKQAHQPYQTRLDQRPETTLFEGYIYQLG
                     NNEHKDSAIQFEKLTYYNENESVMNYRRKAASTRTLWDSEQPRDIAELQWRLSRPVAT
                     ILLALIAVSFTRTIPRQSKSDKTFLIAAMVFALYYNLSGLAQTWVEQAVIPEIPGVWW
                     LYSLLLIAVILFLPSIRQKIANLS"
     gene            17575..18636
                     /gene="lptG"
                     /locus_tag="PK522_02185"
     CDS             17575..18636
                     /gene="lptG"
                     /locus_tag="PK522_02185"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HNC40574.1"
                     /translation="MKTAYSYIAKQVIIGMVIATFVLLPLFSFFDLLDQLDDVGKGTY
                     RTADAFLYVAFLLPRRFIQIAPFIALLGTVGALGKLAVNSELVALRIAGLSPLKISLA
                     PVSIGLLLLVLIMILEQFVAPQLHQRAISHRSIALDKSAELGKGLGIWARDEQHILRI
                     GEMLPTQQAIDIEIMYFDHDGYLSTHTYAEFATIFEDYWELNEVTVRTFSANKISSTQ
                     ASSVAWQSFLNADDIGTLTKPPESLSPAELMRHAAFLQSTGQKANAYIMAIWRKIGGG
                     IMIIAMILLAIPFIFGSIREGLGGKLILAAVIGVSVYLLDQIIANAGLLLHMNPVLTS
                     ILPSVALISVAIYFLKRVY"
     gene            18663..19892
                     /locus_tag="PK522_02190"
     CDS             18663..19892
                     /locus_tag="PK522_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNC40575.1"
                     /translation="MSTNTYIYIYEDSEVKLWGLSGRERLERMVQSFQHINLIDDPLK
                     TPDDSPVLLLRADHLFDIRVISTLIHLEGEIVLCNQAGTPVALRTVGNRVPSRIEALR
                     HTTRLSAIHGMKQYSIQDLQVGLQQKLKKKEHPYVLPIAVNQKEALEWELFAGSYKGV
                     TDLVTKWLWPIPAFWVTHLCIRWKITPNQVTVLSIVLALLAGIAFWYGHYASGLIMGW
                     IMTFLDTVDGKLARVTITSSRIGDVMDHGLDLIHPPLWYLAWGIGLTATELPLANLEF
                     LVWLIFIGYIGGRICEGLFEFWLAPFTLFIWQKIDSFNRLITARRNPNLILLTASWFV
                     GRPDIGFILVAGWHILSTGFLAWRLFKAWQAKHEQGTLTSWMEAIDPVLDRKQIAVKV
                     FTRVPLAEKDDQNRARA"
     gene            19879..20874
                     /locus_tag="PK522_02195"
     CDS             19879..20874
                     /locus_tag="PK522_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HNC40576.1"
                     /translation="MHALSSTSTSTSLRIGLIYNPLGGLARKHHHAISTILSELPEVL
                     QWEISTVDDFDNAVNALIQSKIEWLIIIGGDGTIQGVFTRIFSRLPTSQWPSISIIPG
                     GTTNMTAFDLGIHDKPNLILERLKQHLNQSTNNSVLKTINKAVMRIEQTGQPTLYGMF
                     LGLGLIARGVKFSRSRIKQIGITGNIFTTIIVLRSLIGTFLGRPHPTWAPVQIMLPHT
                     NEANRSQPYLLAMISTLDRILMGIHPYWGQEKASLHATFVAQHCTRFWLAMLKIILKQ
                     GRSLKKEHGYLSYNTESLELLLDDEYIVDGELFYASSQQGPLKISAKEMVKFLIL"
     gene            20904..21860
                     /locus_tag="PK522_02200"
     CDS             20904..21860
                     /locus_tag="PK522_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40577.1"
                     /translation="MKSPQLPPALIEEMAALSAQAVSIEFDAFVSALKQRFGDSLDAI
                     LLYGSCLHTTDLAEGVADFYVLVDDYQNAYAEHYLQFFNRILPPNVFYLEVNYQEKIY
                     RSKYAVLTTSHFEKAASDWFHPYIWARFAQPSRILSARDEIIRQRINTALAQSVVKFL
                     RTTLSAQPENTLPVEAIWTNGLMLTYAAELRTEKQTRAQKLTQLSLNEYTRLTTAALP
                     ALQDMMERSESQHYYHIRSTRFKNNLTKFQWWLRRWQGRILSILRLGKATLTFRDCAD
                     YAAWKIERHTGVRIEVTPMLRRHPILLGYKIIWKLFRRGIVR"
     gene            21900..22556
                     /locus_tag="PK522_02205"
     CDS             21900..22556
                     /locus_tag="PK522_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HNC40578.1"
                     /translation="MRQLFRWSTWFTYGLLLTLTACSTMYYSGLEKIGIPKRDVLVHR
                     VEKARDTQEETKEQFKSALEHFTAVTNFKGGDLEATYKKLNDEYESSIAKAAEVRDRI
                     SDIEDVSQALFNEWEQEIAQYTNQAMKSSSQQKLTATRSHYKQLINTMKQAETKIQPV
                     LAVFKDQVMFLKHNLNAKAIASLKGELGTLQSDVSTLISSMEKSINEANSFISKMEQK
                     "
     gene            complement(22730..23203)
                     /locus_tag="PK522_02210"
     CDS             complement(22730..23203)
                     /locus_tag="PK522_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40579.1"
                     /translation="MYTINHNKTIEIVNFLLERYCSLGYPVDIKETTKVLSLSKDDVL
                     ISGVCIDKATDGRIFPYTVMDNFLEELMCGESCCSVGRFVGNSKMTLKDIVFFWSYIH
                     QFMLSEGITRLLSTATERHARHYSNLLNWEIYSAPIYIPEYRVRARLLKYDLKQF"
     gene            complement(23397..23870)
                     /locus_tag="PK522_02215"
     CDS             complement(23397..23870)
                     /locus_tag="PK522_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HNC40580.1"
                     /translation="MPIIFLLIFLGCAGLIGYAIYLQLFEDLLPCPLCVAQRIAYWVI
                     GITALLAYFNQTRSAIVKICRGLVIFFAMIGAVIAGRHAWLIRFPESFECGISPEEAF
                     LNALPLAQWWPTMFEANGDCADTSWKFLFLTIPDWSLITFMLIGITALLSGLKRK"
     gene            complement(23887..25095)
                     /locus_tag="PK522_02220"
     CDS             complement(23887..25095)
                     /locus_tag="PK522_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HNC40581.1"
                     /translation="MDDLIFFATCPRGLESVLATELAQLSGTSIEVVQGGVIFRGNWQ
                     TCYRVNLESRIASRILWRIAQQNYRSEADIYRITFQLPWQTWFSPKLTIRVKVSAIKC
                     ALRSLDFVTLKIKDAVCDKFRKIKNTRPDVDTANPDIRIHGFLDQQQLTLYLDTSGEA
                     LFKRNLRKSSGNAPIRENLAAGILALTGWKPGVPLLDPMCGSGTFLMEAAQISSRIAP
                     GINRHFAFEQFTLFDSPVWKQLKEAALNQQLPCTSQLIFGSDLSGYAIADARENLISN
                     HLAEIVQLKQANILEISPPHQLGGIIVMNPPYGVRMSEQQLLADFYPKLGDRLKKKFS
                     GWCAYILTADPSLPKLIRLNPSRRTPLFNGALECRLLEYRMVEGSMRKGEPRSSDSVS
                     ANLAVISSDQ"
     gene            complement(25136..25270)
                     /locus_tag="PK522_02225"
     CDS             complement(25136..25270)
                     /locus_tag="PK522_02225"
                     /inference="COORDINATES: protein motif:HMM:NF016156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNC40582.1"
                     /translation="MFFSGTLYIVSFTEMRSLGMVAPIGGLSLMSAWLILAYAIWSEK
                     "
BASE COUNT         7489 a         5479 c         4904 g         7406 t
ORIGIN      
        1 aatggatgaa tttataatag atgccatatt tagtgttgtt tttaagtaag tctatgaata
       61 gtagtaatta agttataaaa atttgtcagc aatttcgcgc aagtagcgat tcgactcatt
      121 ctctgataat ttgctaacta tcaatcaacg attaacttca tttcatggat acccttgagc
      181 aacaattatc tgaacgattg ggacgcattc atgcacggca gcgactcggt atcgaaagcg
      241 atcacaagca acaagttttc ggaaaagggt tgaatttttt tcacccagaa aactggtacg
      301 ctacacatac gctaattcga tttgccattc aattagttgg cttgtatggg cgcggtcaac
      361 gtaatgcacg taatttacaa gtcagagaga actcgttcta tttagcgcac ttgcccgaag
      421 catttcatca attccgtatt ttacatttga ccgatttgca tgtggatatg gatgatcgta
      481 atcttactgc gctcatcgaa acgatcattg atttacccta cgatatttgt gtgctcactg
      541 gcgattatcg cgcgcaaacc tttggcacta tcgatcacgc tattgcaggc atgcagcggt
      601 tgattgcgat gctcaagcaa ccgatttacg gtgtacttgg caatcacgat tctattcgta
      661 tgctacccgc gctggaatca atgggcgtga ttatgttaat gaatgaagca gtcaaactag
      721 aaaaagatgg tgagtcgatt tttttagcag gtgtcgatga tccacattat ttccgtgtgg
      781 ataatcttga aaaagctgct caaaccattc cacacgacgc ggtatcgttg ttgctgtcac
      841 atactcctga aatttaccgc caggctgccc atgctgactt tgatatgatg ttatgtggac
      901 atacccatgg tgggcaaatt tgtttacccg gtggcattcc tgttacgctt gattcgagct
      961 gccctcgcta tttaggggcg ggcaaatggc aatatcgcac gatgcagggt tatacctcag
     1021 tgggtgctgg cacttcgatt gtcaatgctc gttttaattg ccccccggaa gtcactattc
     1081 atcagctgtt aaaaaactaa taaggtgttt tacgtatccg caaccaaaat aagatacgtg
     1141 acagcatcag ttcgacacag aaaaaagtca atacaatccc aacaatttgt gaaaagctaa
     1201 gtgttagcca aaactggatt cccagtaatg gaaacaagga ttcaggaatt tgatcgatac
     1261 ctagcgccat tgcgctcggt ttgtatccta atcgccgttt gacaaaactg gaagttagat
     1321 caccaaccat cgataacaat ccgaacgtga tactcattac gataggtaat tgcaataaac
     1381 ttcccgcccc gatcgatacc agtagcgcaa ccaaaatccc ccgaattgtc ttggattgac
     1441 cgaatagcgg ttgaccatca cgccacaagc gatttccatc gattggtata ttgaagcgct
     1501 ttcccaatat ttttgctaac aaaatgggcg ccccattcac acagatcaac caaatcagca
     1561 atgtaatttc caccattatc ttttctttac ctttgaatgt tgtcagtcat tttagatttc
     1621 cacatttatt tttgacaaaa attttgttga catgggattt tttcacacat atactgaaaa
     1681 aagcgtggga aatatatccg cgtttattga ttgtttattg cataaatctc actgttaaat
     1741 aatttaaagg agtcaaaaaa tgaaaatcgc cctgtttaaa gtatcaacca ttgctgcaac
     1801 cacattagca gtaatggcgc tatcgctacc tgccagtagt gccgaagctg ccgatatccg
     1861 cgttcaatgc gaaaaacgtt ccaaccgctc taaagtttct gttgatggaa acaacctgat
     1921 ttcaggtaac tacagtgcaa aaatcacttc gggcgcaaat accaaagtat ctgcttacaa
     1981 accctcagtc ggtgatgaaa ttgaatttga ttttgatagt gaccgtaatg acgttgctgc
     2041 tggtgccatt cgtatttcaa gcagctttat tcagaacggg caagtaacgg gtgaattgat
     2101 taatgagaaa ggattcacgg ttgcatctag taccagcgca tgccgcatta aataacgatt
     2161 aatttgttac tttttgggcc ctgaaatttc ccctctattt tttcagggct tttttttcaa
     2221 cttatcgatt gctggataaa ggtaattcaa cctgatgcag atcttccaaa ggttgcattt
     2281 ctgcaagtgc gactaaggag ctaacccgta cgccaagtaa acgtatcttt ttatctaact
     2341 tgacacggcg taagcagtta cgggttgctg ataaaatcgc agtagcatca atcactgact
     2401 ttcctagcgt aatatcacga gtgatcgtat taaaatcctc aaaacgaatt ttgatgccaa
     2461 tcgtccttcc ttgataggcc ttacgattaa gatcattggc aacgcgttta caaagggcaa
     2521 tcaattctgc tgtcaaaatt gatcgatcac gaatcgggtg taaatctcgc tcaaatgtcg
     2581 tttcacgact cactgatttg ggttcagaac acgttaagac aggacggtca tcgatccctc
     2641 gggcagcttc atacaaccaa attgcgtaat tatggctaaa gtggatttgt agccagtgca
     2701 actctgcttc ggctaattga ccaatagtgg ttattcccag tgcaataagt tttttctccg
     2761 ctttcgggcc gataccattt atcttacgag ctggaagtgg ccaaatacga gttggaatat
     2821 cttctgcact caatattgtc acgccatcgg gtttattgag atctgatcca atcttggcaa
     2881 gtaatttgtt cggcgtaatc gcgatcgagc aagtcaaacc ggttgcattc agcactgctt
     2941 gtttaatacg ttttgcaagt gtatggttat cgtctgctag ggtggttaaa tcaatataaa
     3001 tctcatcaat gccagaatct tcaatttcag gtgcaatttc aacgacagct gctttgaata
     3061 atctcgaata gtggcgatat gcggtaaaat ctactggtaa taaaattgct tgaggtgcta
     3121 attgtgcagc tttcatgaca cccatagcag aataaatacc caatgcgcga gcttcatacg
     3181 tcgaagtagt aatgacacca cgtcctatgt aatcgctcaa acgcgaaaat tgtaattgac
     3241 catctgcttg aattttcggt ttatgttccg ccctccccgc aatcacaaca ggcaatcctt
     3301 ttaactgcgg ataacgcaac aattcaacgg atgcatagaa tgcatccata tcgagatgag
     3361 caatgcgtcg agatgattga ttcactccaa ttaaattgat tagttgtaag taaaattact
     3421 aaaagtttat tttatcgtga tgaataagaa atcatgctta gcaacttaca taaacttaat
     3481 tttgacaggg tttttcctat taggaattta tgttaatgtg ccctcgttct aattaatgtc
     3541 gatcaaaaca ctcattcatc acaacaagga gttgcttcat gttcacgaaa atcttcaaag
     3601 gattaggtct aagcttatta gttacgggcg ttttggtcgg ttgtactaca acccaaaaaa
     3661 taaccagttc agcaagaaca acgactgagc agctattaat cagtgaagcc ttaaaacgta
     3721 gccttcccaa acaaactaat gcaccattac cgattccaca aggttcagtt gtaaaactcg
     3781 atgtgatagg actgactggt gataaagaca tagtcaaagg tgctgtcgct ggatggcttg
     3841 ggcagcatgg ttacatcgct caagacgaac cagcaacgta tcgcatgaat atcatggtag
     3901 attcgattgg aaccgaacaa gccaatacgt tctttggtat ccccccgatc tctgcggcgc
     3961 tgattccgat ttcattgcca aagctcgctt tctatgaagc tgagtatcaa acgggttatt
     4021 ccagaatcca tctcgatatt tttgaagtac cgtccggtcg attcgttgga tcaaccagtt
     4081 cctttattgg tgatacctat tttaatgccc atacgatact gtttctattt aaactcaaga
     4141 gaacagacct aacttcacca ccagaagttg gtaccttcat gggacactaa atccagccta
     4201 atccaaaccc ctgtgcttta gaaaaacaat cttttcctaa agcacaaaaa acttaaaact
     4261 tttcagcaca ttatttgagt gcatgtggaa tagctgcttt caaataataa atcatcgacc
     4321 acagcgtcag aaacgcagca aggtaaataa accaagtacc cacgagatga gaattaaatg
     4381 attctccaat cgtttcatgg tatagcagca atggaatggc aatcatttgt gcagtcgttt
     4441 tgattttacc cataaatgaa acggctacac tctttgcttt accgatttga gccatccatt
     4501 cacgaagtgc cgaaacagta atttcacgac caataatgat caaagcaatg ggtgcatcga
     4561 gtcgttctaa atgcaaaaga ataatgaggg ccgctgccac cattaattta tctgctacag
     4621 gatcaagaaa agcaccaaaa gctgaagttt gattgagtgt ccgtgcgaga taaccatcta
     4681 accaatcggt aatggctgct acactaaaaa taatggtcgc cactaaattc tggccaaaag
     4741 gcgataatcc aggaatataa aaaatcccga caaataacgg aattgcgaaa atcctaagcc
     4801 aggttagaag gttaggtaaa ttaaagggca ttggataatc agtaagaaaa agtaatgact
     4861 agtgagatgc attgaaaatg aatgtacgga tgcgcagtgt aatgtcaatg taattcccga
     4921 taaatacgtt ctgccaatgc ttgactgatg ccttctgttt gctgtaattc ttcaacgctc
     4981 gctgttaaca cgcctttcaa tcccccaaaa cgccccaata aactctggcg acgtttagca
     5041 ccaatgcctg cgatctcttc gagtgttgag acggttcttg ctttatttct tctcgcacgg
     5101 tgaccttgaa tcgcaaaacg atgcgcttca tcgcgaattt gctgaatcag atgcaaacca
     5161 ggatgatcgg ctggtaattt taacggtttt tctcctgatg gaaaaactaa ttgttcaagc
     5221 cctggtttac gatcttctcc ttttgccacg cctagaaaag gaatatctgc tagccccaat
     5281 tcagtcaaaa tttcctgtgc tacactgact tgacctttac cgccatcgat tagaatcaag
     5341 tctggaattt taccctgacc ttctgcaact ttagcataac gccggtgcaa cgcttgcttc
     5401 attgcagcat aatcgtcacc cggttcgaca cccgtaatgt tatagcggcg atattcatta
     5461 ttttgcatgg caaaatgatc ataaacaacg caggaagcaa ccgtagcttc tcctgcagta
     5521 tgactaatat caaagcactc aatccgatta agatttgata aatttaaagc ctgctgcaaa
     5581 atttgcaacc ggtcttcttg actcgcttgc cgctgcaatt gttgttttag cgctagatca
     5641 gcattggctg tagccatgtc cagccacata cgacgttccc caatcgagtt gctaataatg
     5701 gtaactttcc ggttcgcttg ctcagtcaac aacgattgca gtattttcgt ttgaatttta
     5761 gtatcaatga tgatgatagg aggaatactt cggtttaaat agtgctgcgc aataaatgct
     5821 tcaagcacat caaccatttc gtagccttgt atattttgtg ggaaaaagct tttatctcct
     5881 aagtgtaaac cacctctaat cattaccaag ttgacacaga ccttctctga tccatcggat
     5941 gctttgacac ctgcaatcac atcagcgtct aaggatttcc cgctatcgac aaattgcttt
     6001 tcttggattt tccgtaacgc ctgcatttga tcgcgaaaga cagctgcttg ctcatagtcc
     6061 atctgatttg atgcctgctg catcttatct gcaatatttc taattacttc gttctgttta
     6121 ccctgcaaaa aaagcgaagc acttttaaca tctgcttgat aatcagattg ttcgatcaag
     6181 ttaacacagg gcccactaca gcgctttatt tgatagagta agcaaggtcg agttcgatga
     6241 tggaaaacac tatcctcaca ggtccttaac cgaaaaacct tttgcagcaa ttggatactt
     6301 tcacgtacaa ccccagcatt gggaaaagga ccgaagtact ggtggcgatg atctattgtg
     6361 ccccgataaa aagccaaacg aggaaaagga tgttgactta gctttacata aggatacgat
     6421 ttatcatccc gaaacaaaat gttatatcga gggtttaaac ttttaatcag attattctca
     6481 agtaacagtg cttcagcttc tgaacgtgtc accgttgtct caattcctgt aatttgtgac
     6541 accattaact gaatacgtgg ccctagcatc gatttctgaa aatacgatga aacccgtttg
     6601 cgaagatcga ttgccttccc cacatagatg acctcacctg ctgcattcag cattcgatat
     6661 acgcctggta aatttggtaa tgccgcagca aaaaccttac catcaaatgc aacttcagac
     6721 acgatgttta atgcttatta atcaatcgtc acccaacaat ttaccagcga tagcccacgt
     6781 atcatgaggc atttcatcaa aagaaatata cgtatacgat ttgggcttat taccgattcg
     6841 ttcgagtgta tctgtaattt cttcagcaat tttttgtttc tgttcacgag tcaatgtacc
     6901 tgcaacacga atattcacat aaggcattcg atttctccta agattaatga ttgggaatag
     6961 ctaagtcggc acaaatttct gaaaacacct gttcgaacat ctcctgagtc aggcgcttgg
     7021 tttgagtatt gtagcgacta caatgataac tgtcataaag cttgagttca ttgggtaaaa
     7081 cataaattgc cccatggcta aatgggtagc gctttaattt taaattaaac gccatcaaca
     7141 cggcctggtg agccacattc cccagcgcca gtatcccttt gccaccctgt tcaatgaaat
     7201 gtattatttc attcgataaa taatcattgc attgcttgat ttcacagggc ttaggtttat
     7261 tttcaggtgg cagacatttt actgcattcg taatacgcgt atgaagcaag cacaatgcgt
     7321 cagtgctcga aatcgattca ttctgatttg ccaaaccaaa tttgaataac gtttggtaaa
     7381 gcaaaatgcc tgcataatct cctgtaaaag gcctcccagt gcgatttgcg ccatgcatcc
     7441 ctggcgcaag accaacgatc aacagtttcg gattgatatc tccaaatgct gcgactggtc
     7501 ttgcatgata agttgggtat tgtgctttta cagcatctaa gaatgaagca agacggctac
     7561 agcgtcgaca atgaaccaac gacgaatcaa ttcccatcga tagaaaattt agattaatcc
     7621 taaattacac ttcttagatt tagaaaaact taaccatcag ataaatataa ccaacagcaa
     7681 atactaatgc gacgacaatg gcaacttttt tcaacataaa cgatttcctt tcattccatt
     7741 cttaaattaa ttgatatttg atattttatt atcaatctgc acagattgaa aaaaagagct
     7801 tcattcattt acccaaacta acaatattta ttgatcacaa tttcttaaac ccacgtaaaa
     7861 taggctcttt tcaaacataa tatgcgctca ccaatttgtg gttaggcttc tttcccaaat
     7921 aaattgaatc tactcaaagc actggctacc gttagtagca tgacattgat ttcccgtatt
     7981 ctaggatttg tacgcgatgt attgatcgcg agaatttttg gtgcaggtgt cgctacagat
     8041 gctttttttg ttgcattccg aatccctaac ttattacgca gattatttgc ggaaggagcc
     8101 ttctcacaag cttttgttcc cattctagcc gaatataaaa ataaccggag tgaagctgaa
     8161 acgcgagcat taatcgatca tgtgacaaca ttattaggcg ttacactgtt tgttgttaca
     8221 ctcattggta tcgtagcagc tcccttgatt atttatatca gcgctcctgg attctctggc
     8281 gatattgaaa aattctcgct cactgtcacc cttttgcgca tcactttccc ctatatttta
     8341 tttatttcat tggttgcact ggccggtggc atcctaaata cctacagtca attttcagta
     8401 cccgcattta ccccagtctt attgaattta tcattcatcg gctgctcatt atgggtcgtt
     8461 ccttatgttg atcctcctgt acttgcactc gcatgggctg tttttttagg ggggatttta
     8521 cagcttctat tccaaattcc ttatttaatt cgaattaagc gcatgccgcg catacgattt
     8581 aaaaccaagg ataccggcgc agggcgagtg cttaagctaa tgttacctgc tatttttggc
     8641 gtatcgattg ggcaaatcag cctattaatc aacacgattt ttgcttcttt actcatcaca
     8701 ggaagcgttt cgtggcttta ttacgcagat cggttaatgg aatttcctgc gggactttta
     8761 ggtgtcgcac ttggaacggt tttgctacct tcgttatctc ggcattatgc gagtcacagt
     8821 aacgaagaat actcgcgtct gctggactgg ggattacgtt taacggtttt attgacttta
     8881 cctgctgctg tggcattagc attgcttgca accccactaa tcaccacact gttttattat
     8941 ggtgcattta cagcggaaga tgtttggatg acacggcaag cgctcattgc atatagcttt
     9001 ggattactgg gactcattat ggttaaagta ctcgcacctg gcttctatgc gcgacagaat
     9061 atcaaaacgc cagtcaaaat tgctgtatta acactcgctg ccacacaagt catgaattta
     9121 atatttatca tcccgttgca gcatgctggc ttagctttag cgattggttt aggtgcttgc
     9181 cttaatgcag gactacttta ctataagctg cggactaacg atatctatca acccttacct
     9241 ggttggctta aattcatggt taaaattcta gttgccgtga ttgttatggg caccaccctt
     9301 tggttgagta gtggaaatga cagcttatgg ttacaaatga atgccatcga ccgtgcactc
     9361 cgtttaactt tcacggtttg tattggggca attgcttact ttataacact gtggctctta
     9421 gggtttcgct taaaggattt ttctcaactc ggtactagtt aattatcaaa cttatatttg
     9481 tttccgatgt ttatctcgtt caattaaagc ataagccgag tggttgtgta tggattcaaa
     9541 attttcagat tcaaccacat aagcatcgat tcgatcatcg tgattgagta cttcagctac
     9601 atcccgaacg atgtcttcaa caaatttagg gttatcatac gctctttccg taacatattt
     9661 ttcatcgggg cgtttcaata acccatataa ctcgcaagaa gcttgctctt ctgcaatttt
     9721 aataacatct tcaatccaaa caaaactatt cgtgcgaact gcaacagtaa cgtgcgaacg
     9781 ttgattatga gcaccataat cagaaatctt ttttgaacaa ggacaaagac tcgtaactgg
     9841 caccacgact ttcattgtaa aacgatactc tccttgcttt acctcgccaa tgaatgtaac
     9901 ttcataatcg agtagacttt gtacaccgga tatcggagcc gacttattaa caaagtatgg
     9961 aaaggtcatc tcaatatgac ctgaatcgga ctcgagtcga gtaaccatcg cccttagaat
    10021 atcttcaaac gattccaccg atatttcgcg ttcatggcta ttcagtattt ccacgaaacg
    10081 tgacatatga gtgcctttaa aattgtgagg taaattaaca tacatgttaa acattgctac
    10141 cgtatgctgg actccccctc ctttatccgc cactctgact ggatgtcgta tcgctttgat
    10201 accaactcgg tcgatcgcaa tttggcgtgt atcgagtgaa ctttggacat ccgcaatcgg
    10261 catatcaatc tgtttgttca tgttgattcc tctgaaaata gtcattactg cgacgttgct
    10321 agaattcttg tgttttttct gaaaggtaac cgcattgcta tacccaacat agaccgcgtt
    10381 acaagtaatg tgactacctt caattcaaat ggttcttagt agcaattcac tacaacggaa
    10441 tcacttagcc agcctatctt ggatcgattt tagaatgccc gccttatcta acccacaatc
    10501 tgcaagcatc tgtactgggt cgccttgatc aagaaaaaca tctggcagcc ccagttgtag
    10561 aacattcaca gtaacactac tcgccatgag tgattcaaga atagcgcttc ctgctccgcc
    10621 catgatggta ttttcttcaa cagtcactaa taaatcatgc gttttagcca aatcactcac
    10681 cagttcatca tccaacggtt ttacgaagcg catattggca accgttgcat caagcacttc
    10741 gcctacttct aaacagggtg taagtaaagt tccaaaagct aaaatcgcaa ttttactacc
    10801 tgagcgacga atctctccgc ggccaatcgg taacgcattc atttttttct ggattttaac
    10861 gccgatacct gaaccacgag gatagcgaac agcagaaggt gtattgagtt ggaacgcagt
    10921 atataacatc tgccggcact cattttcgtc agcgggcacc ataactgtga tattagggat
    10981 acatcttaaa tagcttaaat caaaacttcc cgcatgcgtt ggtccatcag caccgaccaa
    11041 cccagctcga tcaatcgcaa atacaactgg caaattttga attgcaacat cgtgtatcag
    11101 ttgatcatag gcgcgctgta ggaaggtcga gtaaatcgct accacgggtt tcaatccatc
    11161 acaagctgcg ccggctgcaa aggtaactgc atgctgttct gcaataccga catcaaaata
    11221 gcgattaggg tattcttgag aaaatttgac taaaccggag ccctcgcgca ttgcgggggt
    11281 aacaccaatt aaacgcgtat ccagtgcagc catatcacat agccaatctc caaatacttg
    11341 agtataagtt ggtttgcttg cggctttggc aataataccc ttatgaggat caaactttcc
    11401 aaccccatga tataaaatag gatcttcttc ggcaagctta taacctgcac ctttgcgcgt
    11461 gacgacatgt aagaattgtg ggccatcgag atgcttgata ttgctcaatg tcgtcaataa
    11521 aacatcgaga tcgtgtccat cgatcggccc aatatagttg aagccaaact cttcaaataa
    11581 agtacctggc gtcatcattc ctttcacatg ttcttcagcg cgtctcgcga gctctaaaac
    11641 cggtggtacc acccctaata ctttctcacc cgctcggcgt gccgtggcat aaaatcgtcc
    11701 agacatcaat ttagccaaat aattattgag tgcgcctaca ggaggcgaga tagacatatc
    11761 gttgtcgttc agtatcacaa gtaaattcgc atccatcaca cccgcattat tgagcgcttc
    11821 aaaagccatg cctgcactca tggcaccatc cccgatgatg gcaatggctc gtctagtcgc
    11881 atcatccagt tgagaagcaa cagccatgcc aagggcagcg ctaatcgacg tactcgaatg
    11941 ggccgtccca aatgcatcat attcgctctc gtcacgacga ggaaaaccag cgacacctcc
    12001 tttcatcctt aacttggcca tcccagcacg acgaccggta agaactttat gcgcataagt
    12061 ttgatgacca acatcccaaa ccacgcgatc atagggtgtg ttaaatatat aatgtagtgc
    12121 gatagtcagt tcgattgtgc caagattaga agacaagtgt cctcctgtac tggcaaccga
    12181 ctcaattaga aattggcgca attcatccgc gagctgcgga agctgctttc gatctaactt
    12241 tcttagctct atagggctat taatacgatc tagtaatgga tacattcaac acagaataaa
    12301 attattttca aaaattaaga aggtaagatt gtcaaaacta aaagtgacgt tgaatgatga
    12361 aatcagttac ttgtagcaaa cgctgggttg attttatctc gctgagtgct ttgtatgcat
    12421 cttgttgtaa cgattcagcc aattcgcgag cttgcttaac acccaagata ctgacataag
    12481 tcggtttatt ttgttgagcg tcctttccag ctgttttacc cagtgttgcc gtcgttgctt
    12541 ctgaatcgag aatatcatct accacttgaa aggccaagcc aatacatttt gcaaaatgat
    12601 ctaatcgact taaatcgtgt gaagaaagtt gattaccgca atgcgctccg agcatcacag
    12661 ccgctcgaat taatgccccg gttttatgga tatgcatcga ttcaagttct ggcaaggtaa
    12721 tagattttcc gacactcgct aaatcaatgg cctgtccacc agccatacct ctcgatcctg
    12781 atgcttgagc caattgcttg atcatttcta attgatcggt aggattatca gtgacagggt
    12841 attctgccac cagctgaaaa gcgagacttt gcagactatc gcccactaaa agtgcggttg
    12901 cttcatcata ctgaatatgg caagtcggtt taccccgacg taaaacatca tcatccatgc
    12961 aaggtaaatc atcatgcacg agagaatacg catggatcaa ttcaacagcc gctgcaacga
    13021 ccctgacacg agaaatatct gcgccaccga gctcacctgc cgcaaaagct agcaatggtc
    13081 gaacacgctt accccccccc aacacagcat agcgcatcgc atcatgtaaa cgctctggcg
    13141 ctgttctatt gttcggaagc cgctcttcta aaaatagctc taattgatgc tggcaatttt
    13201 taacccactc ctgaaaatta acgctcattg atactttccg tttcagtaaa atcacgtaaa
    13261 ttaccgtctt cgaggacacg aatttgttgc tgtgcatttt gcaaggcttt ttggcaatac
    13321 tgcaatagct ccattcctcg tttataagct gctaatgatg cttccagcgt catttgacca
    13381 ctctccatct ttatcacgat actttctaat tcagcggatg cagcttcaaa gctttccggc
    13441 tcaacgatta atgtttcagt tgtttttttt ttcacgattt ttctaaagaa atcatttgta
    13501 tactttgatt atatccattg aattcaaatc aatccctatg cactgcttct tttaaagcct
    13561 tttaaagctt ttgtttcttt aatgttaaaa aggttaaaaa tctggttatt ttaacctgca
    13621 taattgggtt catgctattt tgattctaaa tagtatttaa aataatagta aattagctta
    13681 cttcagataa tttggacaga atatttttat aaaccaatgg ataaaaaaca tgcaccgtat
    13741 catcctacaa aatcaaaact gctttctacg ctatctgctt taaaacatat tgctactcgc
    13801 tatatcctat ggcaatatca agcaaaatcg atatttaata ctctctcaat ataaaaattg
    13861 agccagtaaa atattacgtc ttactttgtt tataacttat ttttaagaat agaatcgagg
    13921 acattttgga aaattcactt gatcaaatca ttaaatactt tgaagaagta ccactttggc
    13981 catttgttct attagctggg cttcttttat tctcacttgg tattgagttt gtcaatcgta
    14041 ggcgacggat agatgcggtt gaatattttg atagcacatt cctaacagaa ttagctggct
    14101 tatatcccca agctacccga tggcctgaag atctcgctga acaccttcaa gaaagcttcc
    14161 cagtgatgcg tgatgcattt gaaaatttga gaggattcct accccagaag cagttacgtg
    14221 aatacaatgc agcttggaat aatttttttg atttttgccg taccaatggc caaagtcacc
    14281 gtaatgtcaa tgtaaaatct gtatcggatc agccatcaga acttaatcaa tcagcccaag
    14341 atatcgagac atttcataaa ctagtatctg ctttgctcgc ctatagcaac caatttaagc
    14401 ggtagtaaac gattactctt tgcatgcatt gcttgcgttt attggaatct aagctgagtt
    14461 atcactgtaa cttagtcttt taaattgatc atcaaagttt ttttatctga taattaaatt
    14521 gatgagtgag atcgttgttc gtcccgttga tacttatcag cggatgggac atttcattga
    14581 ggtaccctgg ttagtttata aagatgatcc gaactgggtc ccaccccttc gattagaaag
    14641 acgatttcat tactctagat ttaatccttt cttcgaacac gctgaatggc aagcctgggt
    14701 agcttataaa gataatcacg cggtaggaag aatcagtgcg cagatcgatt ctttgcatcg
    14761 gcagcattat gggcagcaga caggccattt cggcacacta gagtgtatta atgatgaagc
    14821 cgtgtttgca acgcttataa atagtgctga gcaatggcta atcgagcatg acgctttcaa
    14881 tattacgggt ccattcaacc tatcgatcaa tcaggaatgt ggcgtattag tggaaggctt
    14941 cgatacccca ccggttgtta tgatgcctca ttcgcgtaaa tggtatgact cgttgctaca
    15001 gcaatatggc tatcaatcag cacgtgatct ccttgcttat tgggttgatg tcgatttcac
    15061 tgcgcccaaa gccatgcaag cagtcaacca gcgctatcgc gatcaaatta aactaaggac
    15121 gctcagacgc aagcatttta atgaagaaat ggagacacta agagatatct ttaatgatgc
    15181 ttggtccgaa aactggggat ttgttccttt taccaaagct gagttctctg aactgggtaa
    15241 tagcctacgc tggttagttc ccgaagatta catccaaatc gcagaactag agggcagacc
    15301 cgttgcattc atggcaggta ttcctaatct caatgagatt ttttcacaac tcaatggtag
    15361 tctctttcct ttcggttgga ttcgattgat taaattactc aaatcccgtg ccattaaaac
    15421 gggccgtatt cctctgatgg gggtaagaaa acaataccaa aaaacaccaa ttgggcttgc
    15481 ccttgccttt gcagtaatcg atgcgcctcg acaaattgga attaggcaag gtatcaaagc
    15541 ggttgaaatg tcatggatcc tcgaagacaa ttcacctatg cgtagtatat taaaaagtat
    15601 tggtagcaaa gaatataaac gctatcgttt atacgagaaa acacttgtag cataacatcc
    15661 gatgaattca aacctaaatt atgctgcgct ggtacttgct gcagatcgta ctgcaaatga
    15721 tcctatcgcg ctaaaaacgc aagtaccgtg taaagctttt gcacctatcg atggaaaacc
    15781 aatgataata agagtactag atacccttat tgcaagccct acagttaaat ccattacgct
    15841 atgtggacca cctcaggccc tgctaccaga gtgcccagaa cttcatgaac gcatcttaca
    15901 tgggcagatt ggttggatta caaatcagaa ttcaccgagt ttaagcgctg caaaagggct
    15961 ggaacaactt gacccagata ttcccgtgct tctcaccacg gcagatcatg ctttactgac
    16021 ccctgaaatc gtcgaatatt ttctaaaatc ggccagtgat aatgaatacg atgcaacagt
    16081 tggtgcaatc aactatgaaa taattcatgc ggcattccct gaaagcaaac gaactgttat
    16141 tcgattacaa gatggtaaat attgtggctg taatttattt gcatttaaac ccagagggcg
    16201 attactggtt tcctattggc agaaggtaga agcccaacga aagcgtccct ggaagctcat
    16261 atcgcaaacc attggatggc aagctgtttt caaatatcta ttgggtttct tgacattgaa
    16321 tcaagcgctt tcgcatatcg aaaaacaaat tgggttaagc gtaaaaatcg ctacgcttcc
    16381 ttttccagaa gctggagttg atgtagacac ggttagtgac ttgatgtttg ttgaagcgct
    16441 gttaaagaaa aaagcagaaa atacttaatt aagcattaca cgtttctctc agagtgaaaa
    16501 gatgaaaata atagaaaaat acatcactcg cgaattattg attccttttt tcgtggtcat
    16561 tggaattcta attggactat tctctagctt tagtatcgca cgttttctaa ccagtgcagt
    16621 gactgaaacg cttggtatga tcgcaatgct taagctcgtc agtcttagaa ccatcattgc
    16681 gttggaagta ctggttccaa tcgccttcta cgttgccatc atttatgggt tgagtcgatt
    16741 aaataaagat caggaattga atattattcg ttcatccggg tttggagaca accgcatttt
    16801 atttacaatt ttggtaattg ctttacctat tagtatcatt agtggcgttc tctctcttta
    16861 tgctcgtcca tgggcatatg cagaaagtta tatcttagat gctcaggcag aagcagaatt
    16921 gaatgcagat cgatttcaag ctggtcgatt ctatggtagt gaaaaaagtg ggcgggttat
    16981 tttcgttcag tcaaaagatg ataccggtca gcacatggaa aatgtatttc attttgtcag
    17041 gaaaggggaa aatagcgaaa ttatcgttgc aaaacaagca caccaaccct atcaaacaag
    17101 attggaccaa agacctgaaa caacgttatt cgagggttat atctaccaac tcggcaataa
    17161 cgaacacaaa gatagtgcga tacagtttga aaagttaacc tactataatg aaaatgagag
    17221 tgtaatgaat tatcgtcgta aagctgcatc aacacgaacg ctttgggatt cggagcaacc
    17281 tcgcgatatt gctgaattac aatggcgact atcacgtccc gttgcgacaa ttttacttgc
    17341 actcattgct gtctcattta cgcgcacgat tcctcgccaa agtaaaagcg ataaaacttt
    17401 cttaattgct gccatggttt ttgcgcttta ttataattta agtggacttg cacaaacttg
    17461 ggtggagcaa gcggttattc ctgaaatacc gggtgtttgg tggctttata gcttgttgtt
    17521 gattgcagtc attttatttc tgccgagtat tcggcaaaaa atagctaatc tatcgtgaaa
    17581 actgcataca gctatattgc aaagcaagtc attatcggca tggtgattgc tacttttgta
    17641 ttattaccgt tattcagttt ttttgattta ttagatcaac ttgacgatgt gggaaagggt
    17701 acatatcgta cggccgatgc ctttctctat gttgcatttt tactaccgcg cagatttatt
    17761 caaattgcgc cctttatcgc attattagga accgtcggcg ccttaggtaa actcgctgtg
    17821 aattcagagc ttgtagcgtt aaggattgcg ggactatccc ctctaaagat cagcttggca
    17881 ccggtttcta tcggactact gctactggtt ctaatcatga tccttgagca atttgttgca
    17941 ccacaactgc atcaacgagc aatttcgcac cgctcgattg cattagataa aagtgcagaa
    18001 ctaggaaaag gattgggcat ctgggctcgc gatgagcaac atattcttcg gattggtgaa
    18061 atgctaccca cgcagcaagc aatcgatatc gaaattatgt attttgatca cgatggatat
    18121 ttatccactc atacctatgc ggaattcgca acaatcttcg aagattattg ggaactcaat
    18181 gaagtcaccg ttagaacgtt ctctgcaaat aaaatttctt cgacgcaagc aagcagtgta
    18241 gcgtggcaat cattcttgaa tgccgatgat atcggaacct taaccaaacc gccagagagt
    18301 ctgtcacccg ctgaattaat gcgacatgcg gcgtttttac aatcgacagg ccaaaaagcg
    18361 aatgcctaca ttatggctat ctggcgaaaa atcggcgggg gaattatgat tatcgcgatg
    18421 attctacttg cgataccttt tatttttggc tcaattcgtg aagggcttgg cggtaaatta
    18481 atactggctg ctgtcatcgg ggtcagtgtt tatttattag atcagatcat tgccaatgca
    18541 ggactactat tgcatatgaa tccggtcctg acttctattt tacctagcgt tgcactcatc
    18601 agtgttgcga tctatttctt gaagcgagtc tattaagtta cttaacaacc acttaacacg
    18661 ctatgtcaac aaatacttat atttatatct acgaagatag tgaagttaaa ttgtggggac
    18721 tatccggtcg tgaacgatta gaacgcatgg tgcaatcatt ccagcatatt aatttgattg
    18781 atgatccgct taaaactcca gatgattcgc ctgtcctgtt attaagagcc gatcatttgt
    18841 ttgatatacg tgttatttct actttaatcc acctagaagg tgaaattgta ttatgcaatc
    18901 aagcaggaac ccctgttgcg ttacgaacgg ttggaaatcg ggttcctagc cgaattgagg
    18961 cgttacgcca cacaacacgc ctttctgcaa ttcatggaat gaagcaatat tccattcaag
    19021 atctgcaagt cggccttcaa caaaaattaa agaaaaaaga gcacccttat gtcctaccaa
    19081 ttgcagtcaa tcaaaaggaa gcgcttgaat gggagctttt tgctggctct tataaaggag
    19141 tcaccgatct cgtcacaaaa tggttatggc ctatccctgc tttctgggtt actcatttat
    19201 gcattcgctg gaaaataact cctaaccaag ttaccgtatt aagtattgta cttgctttac
    19261 tagcaggcat tgctttttgg tatggtcatt atgcgagtgg cttgattatg ggatggatca
    19321 tgactttctt agatacagtc gatggaaagt tagcacgagt cacgattacg tcgagtcgaa
    19381 tcggcgatgt tatggatcat ggcttggatc tcattcaccc tcccttatgg tatctagctt
    19441 ggggcatcgg tttaacggct accgagctgc cattagctaa cctagaattt cttgtttggt
    19501 taatattcat tggctatatc ggtgggcgta tttgcgaagg attatttgaa ttctggctag
    19561 ccccttttac attattcatt tggcagaaaa tcgactcttt taaccgcctt atcacagcaa
    19621 gacgtaatcc taatctaatt ttattaacgg ctagctggtt tgttggacgt cctgatatcg
    19681 gttttatact ggtcgcgggc tggcatatac tatcgactgg atttctcgca tggcggttat
    19741 tcaaggcatg gcaagcaaaa catgaacaag gcacactcac ctcatggatg gaggcgatcg
    19801 atccggtact cgatcgcaaa caaatcgcag tcaaagtttt tacacgagta cccttagcag
    19861 agaaagacga tcagaatcgt gcacgcgctt agctcaacct ctacatcaac atcactgcga
    19921 attggtctca tttataaccc attaggtggt ttagcgcgga aacaccacca tgcgatatcg
    19981 actatcctga gcgaattacc tgaggtttta cagtgggaaa tatcaacggt tgatgatttc
    20041 gataacgcag tgaatgcgct cattcaatct aaaatagagt ggctcatcat tattggcggt
    20101 gatggaacaa ttcaaggtgt tttcacgcgt attttttcaa gacttccaac gagtcaatgg
    20161 ccttccatct cgattatccc ggggggaaca accaacatga cagcctttga tctgggaatt
    20221 catgataaac cgaacctaat cctggagcgt ctcaaacagc atttaaatca atccacaaat
    20281 aattcggtac tcaaaacaat caataaagca gtgatgcgta tcgaacaaac aggccaacca
    20341 actctatatg gcatgttcct cggattaggg ctgattgcaa gaggtgtgaa attttcgcgc
    20401 agccgcatca agcaaattgg catcaccggt aatattttta caactatcat cgtacttcgc
    20461 tctttgattg gcactttttt ggggcgccct catccaactt gggcaccggt acagatcatg
    20521 ttaccacata caaatgaagc taatcggtca caaccctact tactagccat gatcagtaca
    20581 ttagatcgca tcttgatggg gatacacccc tattgggggc aagaaaaagc atccctacat
    20641 gcgacatttg tagcgcaaca ctgtactcga ttttggctcg ctatgttaaa aatcatccta
    20701 aaacaaggtc gatcccttaa aaaagagcac ggctatctaa gctacaatac cgaatcactc
    20761 gaattactac ttgacgatga atacattgtg gatggtgaac ttttttacgc atctagccag
    20821 caagggccgc tcaaaatttc agccaaagaa atggttaaat ttctcatact ctgaggttaa
    20881 aatttgtgat gaatgaaact agcatgaagt ctccgcaatt accaccggca ctgattgaag
    20941 aaatggctgc actcagcgcc caagctgtct ccattgaatt tgatgctttc gtgtcagcac
    21001 tcaaacaacg cttcggggat tcattggatg ccattctatt atatggttct tgcctgcata
    21061 caacggattt agctgagggt gtcgctgatt tttatgtact cgttgatgat tatcagaatg
    21121 cttatgcaga acactatctc cagttcttta atcgcatctt acccccgaat gtattctatc
    21181 ttgaagtcaa ttaccaagaa aaaatttacc gttctaaata tgctgtgctc acaaccagcc
    21241 attttgaaaa agcagctagt gattggtttc atccttacat ttgggcacga tttgcccaac
    21301 cctcacgtat tctctctgct cgagatgaaa tcattcgcca aagaatcaat acagctttag
    21361 cacagtcagt tgttaaattc ctgagaacaa cgctatccgc acaaccagaa aatactttac
    21421 cagtggaagc aatttggacc aatggcttaa tgctaacgta tgcagccgag ttgcgtactg
    21481 aaaagcaaac acgggctcaa aaattgactc aactcagttt aaatgaatat acccgcctaa
    21541 ccaccgctgc acttcccgct ttgcaagata tgatggaaag atctgaatct cagcattact
    21601 atcatatccg ctcaacgcga ttcaaaaata acctgacaaa atttcagtgg tggttgagac
    21661 gatggcaagg gcgaattctt tctattttac gtctaggtaa agccacactg acgtttcgcg
    21721 attgtgcaga ttatgcggca tggaaaatag aacgtcatac cggtgttcgg attgaagtga
    21781 ccccgatgct gagacgccat cctatcctct taggttataa aatcatttgg aaactttttc
    21841 gtagaggtat tgttcgttaa caattacatt ataaataatc ttatctttgg gagaatttta
    21901 tgcgtcagtt atttcgttgg tcaacatggt ttacatatgg gttgttatta accctaactg
    21961 cttgttcaac tatgtactac agtggacttg aaaaaattgg tattcccaag cgcgacgttc
    22021 ttgttcatcg cgtcgaaaaa gcacgtgata cccaagaaga aaccaaagaa caattcaaat
    22081 cagctttaga acatttcacc gcagtgacca actttaaagg tggagatctc gaagcaactt
    22141 ataagaaact taatgatgaa tatgaatcga gtattgcaaa agcagcagaa gttcgtgatc
    22201 gaattagcga cattgaagat gtttcgcaag cgttattcaa tgaatgggag caagaaatcg
    22261 cacagtatac gaatcaagct atgaaaagta gtagtcagca aaagctaaca gcaactcgtt
    22321 cacattataa acaattgatt aataccatga agcaagcgga aacaaaaata caacccgttc
    22381 tcgctgtatt caaagatcaa gtcatgtttc ttaagcataa cttgaacgca aaagcgattg
    22441 cttctcttaa aggtgaatta ggaacgctac aatctgacgt cagtactttg atttcatcga
    22501 tggaaaaatc cattaacgag gctaattcat ttatcagcaa gatggagcaa aagtgatcgg
    22561 agtttaaaaa aatttcacta taactgaaga ggcaggccat tgaacaatcc ggcttgcttc
    22621 agtatcaccc aattacaatt ttttcgttac ttggttagaa gaaaggtaat ttaatgggcc
    22681 catcagattc agtgggtcta ccactaactg aatctaacga atccatcaat tagaattgtt
    22741 ttaaatcata tttcagaaga cgagctctaa ctctgtactc aggaatatag atcggtgctg
    22801 agtagatttc ccaattgagc aagttagaat aatgcctagc atgcctctcg gtagctgtac
    22861 ttagcaagcg tgtaatacct tcagataaca taaattgatg gatataggac cagaaaaaaa
    22921 caatatcttt caatgtcatt ttactatttc caacaaatcg tccaaccgaa caacaagatt
    22981 ctccacacat caactcttct aagaaattat ccatcactgt ataggggaat atcctgccat
    23041 cagttgcttt gtctatgcaa acacctgaaa taagcacatc atctttacta agacttagaa
    23101 ctttggtcgt ttctttaata tcaaccggat aacctaacga gcaataacgt tctaataaaa
    23161 aattaacaat ctctattgtt ttattatgat tgattgtgta catattggcc ccaaaaggag
    23221 ctcataaagc atttactgct aataccattc ctattccgaa atttaatttt caggatatag
    23281 aaataaaacg aaacaaactt aaagattaaa aactaaaata attcattgaa aattcagcgc
    23341 aaagcaatgg tgacataagt tttatttatc ttcaacccac cccaatagaa caaacactat
    23401 ttacgtttca accctgaaag caaagcagta ataccgatga gcataaatgt gatgagtgac
    23461 caatcgggaa tcgtaagaaa taaaaatttc caactggtat ctgcacaatc gccattcgct
    23521 tcaaacattg tgggccacca ctgcgcaagc ggtaatgcat tcaagaaagc ttcttcagga
    23581 ctaataccac attcgaatga ctcaggaaaa cgaatcaacc atgcgtgtcg accagcaatc
    23641 actgcaccta tcattgcaaa aaaaataacc aaaccgcggc aaatctttac aatagctgat
    23701 cttgtttggt taaaataagc cagtaatgct gttattccta ttacccaata agcaatgcgt
    23761 tgtgctacac acaatgggca cggcagtaag tcttcaaata attgcagata tattgcataa
    23821 ccaatcaatc ctgcacagcc cagaaaaatc aataaaaaaa taataggcat atctaactaa
    23881 tgagaattat tgatcagagg aaatcacagc taaatttgct gatacagaat ccgaagagcg
    23941 gggctctcct ttacgcatac taccttctac cattcggtat tcgagtaagc gacattccaa
    24001 tgcaccatta aatagaggag tgcgacgcga aggattcaac cgaattaatt taggtaatga
    24061 tggatctgct gtcaaaatat aagcgcacca accgctaaat ttctttttaa gccgatcccc
    24121 taatttagga taaaaatcgg caagtaactg ctgctcgctc atgcgcacac cataaggagg
    24181 gttcatgaca ataatgcctc caagctgatg gggtgggctg atctcaagta tattggcttg
    24241 cttaagttga acgatttcag ccaagtgatt agaaatcaaa ttttcacgtg catcagcaat
    24301 cgcatatccg gataaatcac tgccaaaaat aagctgagat gtacagggta gctgctgatt
    24361 caaggcagct tctttcaatt gcttccatac tggagaatca aaaagcgtaa attgttcaaa
    24421 agcaaaatgg cggttaatgc ctggcgcaat acgactactg atttgcgctg cttccataag
    24481 aaaagtacca ctaccgcaca taggatccaa taagggaaca cctggtttcc agcccgttaa
    24541 agcgagaata cctgcagcca gattttctct aatgggtgca ttcccagaac tcttccttag
    24601 attgcgttta aataatgctt cgccggaagt atccaaatac aatgtcaatt gctgctgatc
    24661 aagaaagcca tgaattcgga tgtcaggatt cgctgtgtcc acgtctggcc gggtattttt
    24721 tattttccta aatttgtcac aaactgcatc ttttattttg agtgtaacaa aatcaagact
    24781 gcgcaatgcg catttaatgg cgcttacttt tacacgaatc gttaatttag gagaaaacca
    24841 tgtctgccaa ggtagttgaa acgtaatacg ataaatatcc gcttcactac gataattttg
    24901 ttgtgctatc cgccagagta tacgactagc aatacggctt tctagattca cgcgatagca
    24961 agtttgccaa ttaccgcgaa aaataacacc gccttgtacg acttcgatcg atgtaccact
    25021 taactgcgct aattcggttg caagcaccga ctcgagaccg cgcggacatg tggcaaaaaa
    25081 aattaaatca tccatcctac aatcaaaaat ttacattgct aacggtgtat tcacttcatt
    25141 tctcagacca aatggcatag gcaagaatta accaggcgct cattaacgat aaaccgccaa
    25201 tgggggcaac catccctagt gaccgcattt cggtaaagct gacaatgtaa agtgtgccag
    25261 agaaaaacaa tatcccgg
//
LOCUS       DAOQLJ010000015        24281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_690_length_24281_cov_5.812144, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000015 DAOQLJ010000000
VERSION     DAOQLJ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 24281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24281
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_690_length_24281_cov_5.812144"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PK522_02230"
     CDS             <1..534
                     /locus_tag="PK522_02230"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNC40583.1"
                     /translation="LSPGQQVIAAYTPGAFADGVRQRVSPQLYYHYGPLGIMSEYVIS
                     QQTITQGNFSDKVTHDAFQVQISWVLFNGNASFRRIRPNKAIDLETMNSWGAVQLVAR
                     YAELHLDANAFKNDLLNPNLSTQSASDFGVGINWYLNRNIKLQLNYDQTHFTKGAENG
                     VDRPDEKILFSRLQITF"
     gene            complement(569..1057)
                     /locus_tag="PK522_02235"
     CDS             complement(569..1057)
                     /locus_tag="PK522_02235"
                     /inference="COORDINATES: protein motif:HMM:NF025447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4262 domain-containing protein"
                     /protein_id="HNC40584.1"
                     /translation="MPHPKPSNDYERKLVANIAKYGWQCTSVRSPEGDTDSVNFSYTI
                     GLNSSYGQPEFIIFGLPVETAHSILSLVADAAASGKPIDVSQPSSTLIKNYEVVFVPV
                     PRDQFSEYLCSVIWWEQGKDFAAVQIIWPDKNGLFPWHLNAPESFKMSQPILGIYTDT
                     KG"
     gene            1337..3385
                     /gene="ligA"
                     /locus_tag="PK522_02240"
     CDS             1337..3385
                     /gene="ligA"
                     /locus_tag="PK522_02240"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNC40585.1"
                     /translation="MVSPPEAVIQQIQFLRATIELHNYRYYVLNEPTIPDAEYDRLFR
                     ELQQLEQQYPELITLDSPTQRVGTAPLDEFSQFTHRTPMLSLSNAFEAAEVETFDRRL
                     CDEITVHTIEYSVEPKFDGLAVSLVYAQGLLTVGATRGDGYTGEDITHNLRTIQSIPL
                     RLPLDNPPAYLEVRGEVMILKADFIQLNQQQEKKGEKLFANSRNAAAGSLRQLDPNIT
                     AQRRLTFFAYGIGILEPPSESLKRQSDVMTFLTSLHFPIAKEHQIVQGTIGLLKYYQW
                     MMSQREQLPYDIDGVVYKVNDLNDQIKLGFVSRAPRFAIAHKFPAQEAVTQLLDIDVQ
                     VGRTGTLTPVARLAPVFVGGVTVTNATLHNEDEIKRKQIMIGDFVIVRRAGDVIPEVV
                     AIVPEQRSVNVTAFTMPDHCPACGSRAVRLTGETIIRCTGGLFCPAQRKQAILHFASR
                     RAMDIEGLGEKLVDQLVDHAIVRTPADLYKLGIAALAQLARMAEKSANNTLHAIEHSK
                     HTTLTRFIYALGIRHVGETTAKDLARYVGQLDRLMAMNVEQLQQVHDIGPIVAESIVD
                     FFTEPHNREVIEQLRAAGVHWDENPEALEYSPSSKVQGKTFVLTGTLPTLSREAAKTL
                     IEQSGGKVTNSISTKTHYVVAGAEPGSKYHKAIELDIPILDEATLLNLLETQTIKPTS
                     "
     gene            3390..3938
                     /locus_tag="PK522_02245"
     CDS             3390..3938
                     /locus_tag="PK522_02245"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HNC40586.1"
                     /translation="MKETLENAQQILSHAELICPPQSLNLAIERLSKEISDVLSKQFP
                     LVLSVMGGAIVFTGQLLPLLNFPLDFDYVHASRYNHAFQGSTLHWKIAPPEQVSGRVV
                     LVLDDVLDEGYTMTAIRQRVLAMGAKQFYCAVLADKSIGKEKPLQADFVGITLPNRFV
                     FGFGMDIKGMWRNLPAIYAIKD"
     gene            3942..4688
                     /locus_tag="PK522_02250"
     CDS             3942..4688
                     /locus_tag="PK522_02250"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HNC40587.1"
                     /translation="MLAIIGGSGMTKLSCLEISRRQIVRTPYGEPSGPLMFGKINDCE
                     IVFLARHGHGHTIPPHLVNYRANLWALHQFSPQRVVAVVSVGGIRADLVPGSIVVPDQ
                     IIDYTYGRKFTFFDNTDRPITYTDFTYPYCQETRAHLLEAAEKSGELLIDGGVYAVTQ
                     GPRLETAAEVNRLENDGADMIGMTGMPEAILAKELDLPYAAIAAVANHAAGRGTSARA
                     IPLEEVYAVLEKAMISVRNILEHMVIEHGD"
     gene            4678..5211
                     /gene="def"
                     /locus_tag="PK522_02255"
     CDS             4678..5211
                     /gene="def"
                     /locus_tag="PK522_02255"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNC40588.1"
                     /translation="MAIKTVLRMGDPVLLQVAKKVEQFDTPELQTLLQDMHDTMASLN
                     GAGLAAPQIGVSLQVVIFGVDYNPRYPDAETVPYTILINPKITPLSKKMEEDWEGCLS
                     VPGMRGKVPRYTKIRYQGVDQHGNPIDRSVEGFHARVVQHECDHLIGILYPMRIRDFR
                     QFGFTDVLFPGQEIMDD"
     gene            complement(5229..5642)
                     /gene="hemJ"
                     /locus_tag="PK522_02260"
     CDS             complement(5229..5642)
                     /gene="hemJ"
                     /locus_tag="PK522_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HNC40589.1"
                     /translation="MLWIKSFHIIFMVTWFAGLFYLPRLFVYHAMCTDQAGNDRFKIM
                     ERKLFYGIMTPGALLTIVFGIWLWLGYGFAGAWLHAKLALVVVLIAYHIYCRKILLDF
                     KHDRNQHSHVYYRWFNEIPVVILIAAVILVVVKPW"
     gene            5712..7055
                     /locus_tag="PK522_02265"
     CDS             5712..7055
                     /locus_tag="PK522_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40590.1"
                     /translation="MNFRLLSFLLLFYGTSVIASNTYLEDPDSYYARLKQLQPGDHLQ
                     LKPGVYRKGLPIHHLPGNRLAPITISGPSGQQSAIFIGRPGHNTVSIIDSSHITIRNL
                     VIDGIGLPVDGIKCEGHANWAHHITLENLQIYGHDHNQEIVGISTKCPAWNWIIRHNI
                     IKNAGTGIYLGNSDGRAPFIAGLIEYNLITHTMGYNLQIKHQLPRPSIPDIPIEKQHT
                     IIRHNVFSKADQSSTGKAARPNLLVGHWPTDGNGKDDQYLIYGNFLYQNATESLFQGE
                     GRIAFYNNLLVNHQGNAIRLQPRSAQSDHISIFFNTILTSEIDIFLAKPKNKSNSTYW
                     ITHNAIFTGKSATASSLTKNNLVYDYPLAGHYLIQPYLMPGQMNLNLIPQDRMVPKLN
                     INHLKNHLDWNVDFDGEISEQRFGAYTNEKRSRWYPNLEIKPVLTTKQKNNLTTH"
     gene            7070..9250
                     /locus_tag="PK522_02270"
     CDS             7070..9250
                     /locus_tag="PK522_02270"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNC40591.1"
                     /translation="MKTLLVITLLIIYGSLYPGDFLTPEPDALEKFFTDLRWTTSLSD
                     MLGNVMLFVPFGLVSSLVTVTHYHSRTRLVLFFFSGFILAFALQIAQVWLPSRSAILA
                     DVIWNLVGLCIGIIIAHFVKKFTQTRIHALDTKIIIPLATLILWMISELVPLVPAIDL
                     QKFKDALKPLFLEFDLDFPTALLHMAGILLAASVLKVVTHSPLRWLTLILLTVIVGKI
                     TIISLQLDITTLVGLTAGYLFSIALLYLETSKLLAISFWVLLLAGTLERITPFVLASD
                     GTLSLIPFATMLKGSMQTSAVGLSQSLFIYTALLWLTQKMGGNFKGITIAVVIWTVFI
                     ELTQMTLLGRTADITEPLLVIFISWVFAEFQHYTNKTQKQSIPDKPYTDSSYSSPQAI
                     STIPVQGNGFLLVSIILMLGITGAIWFILRLPGIPYNLKELFLGNGNLLFIFIFVQAL
                     FWIGTGAAWVSHKVATSIRPYLILPCWTMLVSLISLILLSISVTQESIADIAGSNNLY
                     WFVMNRDIWGENWKPIFEWIGPDTVSILERIVRYTALYSPLIIFLILFTLPMFEQKKL
                     TIQQSTLLIIVTLLWLWLAKAIAFDWSSTDNLNELIARDGPMGWGGGGYLYALLLVLC
                     FNATLLAKTVNFRLSSIFSFALTIAALPLCWWLLTNGLEANVEKYGNTFSGTQFLLGP
                     DRKQALPEIELFIRWSIVQIGFVTVIGFGIRNYYQIKSLASKIK"
     gene            9357..9746
                     /locus_tag="PK522_02275"
     CDS             9357..9746
                     /locus_tag="PK522_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HNC40592.1"
                     /translation="MSKPFIIFLWLTVALFFFSSQAVMAQGYSTMGSGGRTGQSYIAM
                     SGEKLATGVVNIATGIVELPKTVILTSQRDGAPYGLTVGLITGIMHSVGRTVFGALDV
                     VTFLIPTPPTVRPKYIWENFDKETTYG"
     gene            9916..10272
                     /locus_tag="PK522_02280"
     CDS             9916..10272
                     /locus_tag="PK522_02280"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNC40593.1"
                     /translation="MIFTQDKSEKRTHERVTLRTQATILLSDRKAIKVRTLDIALGGM
                     AIVADGNPNSGVVFNIQLTLPLHTDIPPLFEAKVRVAHSVLSGKEGGFKIGLQFIDLN
                     DAAESMLLQFLEQNSK"
     gene            10481..11995
                     /gene="ubiB"
                     /locus_tag="PK522_02285"
     CDS             10481..11995
                     /gene="ubiB"
                     /locus_tag="PK522_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HNC40594.1"
                     /translation="MRFLRLLKILLIALRFGLDDILLSHAKLKILRPLTLLNPFRTRL
                     KLPRAVRLRLALEKLGPIFIKFGQMLSTRRDLLAQDFADELAKLQDQVPPFPSDLAIA
                     TLERVYEKPVNEVFLQFERQPIASASVAQVHLAVLHNGTEVAVKILRPAIAPIISHDV
                     ALMETTAWLLESIWPDGKRLKLREVVSEFARHLEDELDLVREASNCSQLRRNFLDSPL
                     LFVPEVYWDYCYPEVMVMERVKGIPISHVDTLREHNIDIPQLARMGVEIFFTQVFRDG
                     YFHADMHPGNIFVGSDGRYIAIDFGIMGSLSDEDKNYLAQNFLGFFQRDYRRVAQTHV
                     DAGWAPRNTRVDDFEAAIRAVCEPIFDKPLKEIYFGRILLRLFQASRQFNVEIQPQLV
                     LLQKTLLNIEGLGRDLDPDLDLWKTAKPFLENWMSEQIGWRGLLTHLQKEAPNWATIL
                     PEFPRLIHYHLSQERAQVLEDRLTDLVTQEKRQSRLLALLATLLAALLLVQIYL"
     gene            12050..12358
                     /locus_tag="PK522_02290"
     CDS             12050..12358
                     /locus_tag="PK522_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNC40595.1"
                     /translation="MSYYQKHIFFCVNQRENSERCCNDHHAQVMRDYAKDRIKTLKLS
                     GKGKIRVNNAGCLDRCNEGPVIVIYPDNIWYTYVDQEDIDEIIEEHLINDRIVDRLKI
                     "
     gene            12391..13017
                     /locus_tag="PK522_02295"
     CDS             12391..13017
                     /locus_tag="PK522_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNC40596.1"
                     /translation="MKNAVEQKLFVIGPAGKIETIITFPIDKPSGIAIIAHPHPLYQG
                     NMDNKVVYITNKALIEKGFITAKFNFRGVGASEGKYAEGVGEISDVLAVTRFMQENYG
                     ATLNHALLVLAGFSFGGAIQAFAAQQLNPHKLILIAPSVARMNTPQVADHAGATLIIH
                     GDHDDIVPLQSVIDWANPQGIAVTVIPDAAHFFHGKLTILKQTIMNFC"
     gene            complement(13037..13939)
                     /locus_tag="PK522_02300"
     CDS             complement(13037..13939)
                     /locus_tag="PK522_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNC40597.1"
                     /translation="MLHVTLRQLKVFESVARHLSFSKAAEELFLTQPAVSLQIKQLEE
                     VVSLPLFEQIGKRIHLTEAGQELYQYSRAIAQQLSDMELALDELKGLERGKLNIAVVS
                     TANYFAPNLLAKFCQRYKGVTVSLHVSNRETVLKHLADNLTDLAIMGQPPDGIDITAE
                     SFMENPLVIVAPPQHPLCSQQNIPVKRLEEEVFLVRELGSGTRNAMERFFVEQGIRIN
                     KGMEADTTEAIKQAVQAGMGLGVMSLHTIQLELETHRLKILSVEGFPIIRHWYIVHRK
                     HKRFSNVAKTFKAFLLIEAPALMS"
     gene            14140..15561
                     /locus_tag="PK522_02305"
     CDS             14140..15561
                     /locus_tag="PK522_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="HNC40598.1"
                     /translation="MAVKTYNAGVKEYRQTYWMPEYTPLDTDILACFKITPQPGVDRE
                     EAAAAVAAESSTGTWTTVWTDLLTDLDYYKGRAYRIEDVPGDDTCFYAFIAYPIDLFE
                     EGSVVNVFTSLVGNVFGFKAIRGLRLEDVRFPIAYVKTCGGPPHGIQVERDMLNKYGR
                     ALLGCTIKPKLGLSAKNYGRAVYECLRGGLDLTKDDENVNSQPFMRWRQRFDFVMEAI
                     HKAERETGERKGHYLNVTAPTPEEMYKRAEYAKEIGAPIIMHDYITGGFCANTGLANW
                     CRDNGILLHIHRAMHAVIDRNPHHGIHFRVLAKILRLSGGDHLHSGTVVGKLEGDRGA
                     TLGWIDLMRESFIKEDRSRGIMFDQDWGSMPGVFPVASGGIHVWHMPALVAIFGDDAC
                     LQFGGGTLGHPWGNAAGAAANRVALEACVEARNQGIQIEKEGKAILTNAAKHSPELKI
                     AMETWKEIKFEFDTVDKLDVAHK"
     gene            15627..15983
                     /locus_tag="PK522_02310"
     CDS             15627..15983
                     /locus_tag="PK522_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HNC40599.1"
                     /translation="MSEMIDYKSRLSDPASRKFETFSYLPAMDKEQIKKQVQYIVSKG
                     WNPAVEHTEPEYLMSNYWYMWKLPMFGETDVDRILKETEACHKANPNNHIRLVGYDNF
                     AQSQGTSMVIYRGKTV"
     gene            16102..16905
                     /locus_tag="PK522_02315"
     CDS             16102..16905
                     /locus_tag="PK522_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNC40600.1"
                     /translation="MSEAIKQYLIKDQPYYHPVADEVALYEAAYSVRMPMMLKGPTGC
                     GKTRFVEYMAWKLKKPLITVACNEDMTASDLVGRFLLDAQGTRWQDGPLTTAARHGAI
                     CYLDEVVEARQDTTVVIHPLTDNRRILPLEKKGELIHAHTDFQLVISYNPGYQSLMKD
                     LKQSTKQRFGALDFNYPDHDSETEIVAHETGIDKKIADKLVSIAERARNLKGHGLDEG
                     ISTRMLIYAGSLIAKNVDAHAACRVALVRPITDDPDMRDALDAAVSTFF"
     gene            16942..19269
                     /locus_tag="PK522_02320"
     CDS             16942..19269
                     /locus_tag="PK522_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HNC40601.1"
                     /translation="MSINLSDYTELLESLPPETMALLENTWHDATRVFSPRGLDNYIK
                     GTSALQGLGKGRSLVEAWIDEAPQVAKEIGEDVIGDLATTLLMLASKTSGAVIELIMS
                     TSTTAAKRLGDADLFRNYLQFLNTLLAQAPRGVRPMLDKLDVLFGQLTLGGLRRWALW
                     GAHAHRTNYEEQINYFSLSSKESLAVLQRERKGTLFVDIQRRINIYLRALWARDFFMR
                     PTSGDFETRQGYRPYIENYLIHLPDAYDAHGEIAASEVYRAAAAHAAAHLVETKYPIS
                     AESLNPLQMAVISVIEDARVEALSIKRFPGLRQIWAKLHDANPDMATTIGDYLNRLAR
                     ALLDPDYQDPDEWIVEGRLLFAQAQDRLSENQLSWEIGVQLAHKIMGKKIPFNPRTDV
                     LTAPYLDDNRYFWEFEEFNFERSQTAGYEEPKQIRKYVNVMELANEIDVETAGDDAQE
                     IWVVGTEFFPYEDMGVSFNEMEGKEPVSPPFHYAEWDYQIQLERPTWVTVQEKRAKAG
                     DIVLVDSIATQYKREISRMKFLLDAMQPQGVQRIRKLEDGDEIDINAAISSTIDIRMG
                     QQPDPRIMMRSVRKIRDISVLVLLDLSESTNETVSGQEFSVLDLTRQATVLLADAINK
                     IGDPFAIHGFCSDGRHDVEYFRYKDFNQPYDEQPKAKLAGMTGQLSTRMGAAIRHASH
                     HLKQQRSSKKLLLIITDGEPADVDVRDPQYLRYDTKKAVEEAGRSGAITFCMSLDPRA
                     DQYVSRIFGAKNYMVVDHVERLPEKLPLLYAGLTR"
     gene            19311..19613
                     /locus_tag="PK522_02325"
     CDS             19311..19613
                     /locus_tag="PK522_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40602.1"
                     /translation="MSQQVYAGIDLDENGGLTHLGRIVLDAWVFGILPETETCTGWSH
                     AQMQNLYEEVYAAWEPFAHLPSRLPPELSAKHEKLYGQAITIAKRKGWSAELGEDD"
     gene            complement(19660..20067)
                     /locus_tag="PK522_02330"
     CDS             complement(19660..20067)
                     /locus_tag="PK522_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40603.1"
                     /translation="MDITCYRSQEIKREVRLLPATTYNLACQLITRCTTDHLFVPIRA
                     MQYLAILDKKEFVFIDGERKCWVDIAWQNFQTQNRTSLDQPVAYEVVYYRENQEELML
                     RLQKEFPSALQILANKAAIKSPAQIIPFPVINT"
     gene            20104..21903
                     /locus_tag="PK522_02335"
     CDS             20104..21903
                     /locus_tag="PK522_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HNC40604.1"
                     /translation="MRATRLNKPPANRNDWKTIRSLLPYLWEFKGRVLIALMLLLLAK
                     LANVSVPLILKEIIDALDPAQTIVTLPLFLLIAYGVLRFCSTLFGELRDAIFAKVTQR
                     AIRRVAIRVFSHLHSLSLRFHLTRQTGGVSRDIERGSRGISFLLNFMLFNILPTLLEI
                     SLVMVILISKYDIWFFIITFITLLSYIALTVFVTEWRMIFRRTMNDMDSKANTRAIDS
                     LLNYETVKYFNNEAWETQRYDEHLQQWEKAAVRNQTSLATLNTGQSAIIAIGVTLLMM
                     LAASKVVSGEMTLGDLVLVNAFLLQLYMPLHFLGFVYREIKHSLADMERMFTLLAENE
                     EIQDQPNAIALNISHASITFKNVNFSYESNRQILFDVNFSIPSGSTLAVVGHSGSGKS
                     TLARLLFRFYDTSSGAILINGHDIKQITQKSLRSMIGIVPQDTVLFNESIYYNIAYGR
                     PDASEAEILAAAKSAHIHDFVQSLPNQYDSQVGERGLKLSGGEKQRIAIARAILKNPL
                     ILIFDEATSALDSRTEKNIQHELKQLSKNRTTLIIAHRLSTIIDADQILVMDKGRIVE
                     QGNHQQLLATNGIYAQLWEIQQREEDTTGSITI"
     gene            22106..23038
                     /locus_tag="PK522_02340"
     CDS             22106..23038
                     /locus_tag="PK522_02340"
                     /inference="COORDINATES: protein motif:HMM:NF037256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-containing carbonic anhydrase"
                     /protein_id="HNC40605.1"
                     /translation="MLDSIKKMLQQLMGSSSEDSNQATDITFYTLGKFNLGVGKIGPD
                     NRTDIDQSVLKEMYRRVAAGEFNIPVSGTLPEICVDGRTNRQGSRINAPSAAGGSLTM
                     VYGRDLGDSGALNDKTEMQLTIETMDTLKGKGYTTAVHGDDHSNCGCGACAHAQKVYQ
                     HITDKIDPIANLTGNYGIVISDTEKASIMGKAKNRLNHPSFFAEDRSAIIQACQDHGA
                     NYEELVDAHNELGIAINMRQGTTIDRKAIRTTFGPQYDLFVVDAWTFATAAQELALES
                     NKQDADRIAKAITIQNVATASVLGHSSLPIIPIG"
     gene            23202..23918
                     /locus_tag="PK522_02345"
     CDS             23202..23918
                     /locus_tag="PK522_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC40606.1"
                     /translation="MSNENNGSSWKLEMQILIIEDDFAIATNLYDYLESRGYGVDVAY
                     DGIAGHCLAVTQHFDVILLDLGLPKLDGLTICQKLRQESQIDTPILMLTARDTLDDKL
                     KGFDHGADDYIVKPFALKEVEARIVALHKRHSGKVTSRTLVVGDLLFDPKTMSITFAG
                     SAVKLPPKCIRLLALMMSEPGRVFGRKELESAVWGNTQETSDTLRTHMHVLRRSLSQA
                     GGYDPIETVHGLGYHLISRA"
     gene            23911..>24281
                     /locus_tag="PK522_02350"
     CDS             23911..>24281
                     /locus_tag="PK522_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNC40607.1"
                     /translation="MRKHRLRHGLRQRVALALAIFCIFFVSIFGISLYVVSGDIEEDH
                     IEQILRMEMEHLIYRYEQHHDIRPQIGSNLEGYIITSIADEKKLPSYLQGLNFDYHTI
                     FLRQEEIRVAVKHINEIKFLVA"
BASE COUNT         7076 a         5315 c         4977 g         6913 t
ORIGIN      
        1 ctttcacccg ggcagcaagt catcgcggcc tatacaccag gtgcatttgc tgatggagtt
       61 cgacaacgcg tttcaccgca gctttattat cactatggtc cgcttggcat catgagtgaa
      121 tatgtcattt ctcaacaaac tatcacacag ggcaatttca gcgataaagt tacccatgac
      181 gcattccaag ttcaaatatc atgggtatta tttaatggca acgcttcttt tcgtagaatc
      241 cgacctaaca aagcaatcga tttggaaaca atgaatagct ggggagccgt gcagctagta
      301 gcgcgctatg cagagcttca tcttgatgca aatgctttta aaaatgacct acttaatcca
      361 aatttatcaa cacagtctgc gagtgatttt ggcgtaggta ttaactggta tctaaaccga
      421 aatatcaaac tccaattaaa ttatgatcaa actcacttca caaaaggtgc agaaaatggg
      481 gttgatcgac ctgatgaaaa aattcttttt tcccgtttgc aaataacttt ctgactttga
      541 ttatctaaaa acacagttaa ctagcttatt aacccttcgt atctgtgtaa attccaagga
      601 taggctggct catcttgaaa gattcaggtg cattaagatg ccagggaaac aagccattct
      661 tgtcaggcca aataatctgt accgcagcaa aatctttacc ctgttcccac caaataacgg
      721 aacaaagata ttcagaaaat tggtctcgcg gtactggaac aaaaacgacc tcataattct
      781 taatcagcgt cgagcttggt tgagagacat cgatcggctt acctgaagcc gctgcatcgg
      841 caactagact taaaatacta tgcgcagttt ctactggtaa tccaaaaatg ataaattcag
      901 gctgtccata gctgctattc aaaccgatcg tatatgaaaa atttactgaa tctgtatcac
      961 cttctggtga acgaacactg gtacattgcc aaccatattt tgcaatattt gcaacaagct
     1021 ttcgttcata atcattacta ggtttaggat gtggcatgaa tcattccctg actttcagtt
     1081 caataagttt tttgtagctg ctagctcaat ttaagtatcc tggtctgcaa taattgccta
     1141 cgctaatttg gatatttcaa gctgattctt atgaaatcta tcgaattgat ttagtccgat
     1201 cataccaaat tctatttgac ttataaccta gcgcataatg agattcaacc tactcaccct
     1261 ttacctctgc catgatgact aatggtatga tttggatttt ccaacaccgt cccgtcacaa
     1321 ctaatcaagc ttagcaatgg taagcccacc tgaagcagtt attcagcaaa tccaattctt
     1381 acgagcgacg atcgagttac ataattatcg ctattatgtg ctgaatgaac ccacaattcc
     1441 ggatgctgag tacgatcgtc tgtttcgtga attacaacaa ctggagcagc aataccctga
     1501 attaatcaca ttggattcac ccactcaacg cgtcggaact gcgccgctgg atgagttctc
     1561 tcaattcacg catcgtaccc ccatgctatc cttgagcaat gcgtttgaag cagctgaagt
     1621 tgaaacattt gaccggcggc tctgtgatga aattacagtg cataccattg aatacagcgt
     1681 tgaaccgaaa tttgacggac tagccgttag tttagtgtac gcgcaagggc tacttaccgt
     1741 tggtgcgacc cgtggtgatg gttataccgg cgaagatatc acacataact tacgcactat
     1801 ccagtcgata ccgttacgtt tacccctaga caatcctcct gcataccttg aagtacgggg
     1861 agaagtcatg attctcaaag ctgattttat acaactcaat cagcaacaag aaaaaaaagg
     1921 ggaaaaatta tttgccaact cacgtaacgc cgctgctggt tcactccgac aactcgaccc
     1981 taacataacc gctcagcgcc gactcacttt ttttgcatat gggatcggta tccttgagcc
     2041 gccctctgaa tcgcttaagc gacaaagtga tgtcatgact tttttgacct cgctacactt
     2101 cccaattgct aaagaacatc aaatcgtaca aggtacaatc ggtttactaa aatattacca
     2161 gtggatgatg tcgcaacggg aacagttacc ttacgacatc gatggtgtcg tttataaagt
     2221 taatgactta aatgatcaaa tcaaacttgg ctttgtttct cgagcacccc gctttgcaat
     2281 tgcccataag tttccagcac aagaagcagt aacgcaatta ctcgatattg atgtgcaagt
     2341 tggtcgcaca ggaaccctca ctccggttgc ccgactagca cctgttttcg tgggtggagt
     2401 aaccgtcacc aatgcaacat tgcataatga agatgaaatc aaacgtaaac aaatcatgat
     2461 tggtgatttt gtcatcgtac gaagagccgg cgatgtcatt ccagaagtcg ttgcaatcgt
     2521 tccggaacaa cggtcagtta atgtaactgc tttcaccatg ccagaccact gtccggcatg
     2581 cggatcacgc gcagtaagac tgactggtga aacgatcata cgttgcacgg gtggcttatt
     2641 ttgcccagcg caacgcaaac aagcgattct gcattttgct tcacgccgtg cgatggatat
     2701 tgaaggctta ggtgagaaac tggttgatca attagttgat catgccattg ttcgcactcc
     2761 tgctgatctt tacaaattgg gtatcgctgc actagctcaa ttagcgcgta tggctgaaaa
     2821 atcagcgaac aatacgctcc atgctataga acacagcaag catacgaccc tcactcgatt
     2881 tatctatgcg ctgggtattc gccatgtagg cgaaacaact gccaaggatt tagcacgtta
     2941 tgttggacaa ttggatcgat taatggcaat gaatgtcgaa caattgcagc aagtacacga
     3001 tatagggcct atcgttgcgg aaagcattgt cgattttttt accgaacctc ataatcgtga
     3061 ggtgatcgaa caactccgcg ccgcaggtgt acattgggat gaaaatccag aagccttgga
     3121 atattcacct agttccaagg tgcaaggtaa aactttcgtg ctcaccggca cactccctac
     3181 cctatcacgc gaagcagcaa aaactttgat tgagcaatcc ggtggcaaag ttacaaacag
     3241 tatttctacc aaaacccatt acgtggtggc tggcgctgaa cctggcagta aataccacaa
     3301 agctattgag ttagatatcc ctattcttga tgaagctaca ttgcttaatt tgctagaaac
     3361 tcaaacgata aaaccaacct catgatgtta tgaaagaaac cctggaaaat gcacaacaga
     3421 ttctaagcca cgcggagcta atctgcccac ctcaatcgct caatttggct atcgaacgtt
     3481 tatctaaaga aatcagtgat gtactatcaa aacaatttcc actggtactc agcgtgatgg
     3541 ggggtgctat cgtttttaca ggacagctat taccattgct taactttcca ctcgatttcg
     3601 attatgtaca tgcaagtcgc tataatcatg cttttcaagg ctcaaccttg cactggaaaa
     3661 tcgcaccacc agaacaagtc tcaggtcgag tagtccttgt tttggatgat gttctcgatg
     3721 aagggtatac gatgaccgca attcgccaac gcgtactcgc aatgggagca aaacaatttt
     3781 attgtgcagt attggctgat aaatcgatcg gtaaagaaaa accgcttcaa gctgattttg
     3841 tcggtattac attaccgaat cgatttgttt ttggttttgg aatggatatc aaaggaatgt
     3901 ggcgaaattt acctgcaatt tatgcgatta aggattaatc aatgcttgcg ataatcggtg
     3961 gcagtggaat gaccaagctt tcttgcttgg agatatcgcg ccgtcaaatt gtgcgaacac
     4021 cctatggcga accatcgggc ccgctcatgt tcggaaaaat caatgattgc gaaattgtgt
     4081 ttctggctcg tcatggacat ggccacacga ttcctccaca tcttgtaaat tatcgtgcta
     4141 atttatgggc gctgcatcaa ttcagccccc agcgagtcgt agcagtagta tctgtaggcg
     4201 gtattcgcgc tgatttagta ccaggcagca ttgttgtacc tgatcagatc atcgattata
     4261 cctatgggcg caaattcacc ttctttgata acacggaccg ccccattacc tacacggatt
     4321 ttacttatcc ctattgccaa gaaacacgcg ctcatttact agaagctgct gaaaaatcgg
     4381 gtgagctcct tattgatggg ggcgtttatg cagtcacaca aggtcctcgc ctcgaaacag
     4441 ccgctgaggt caatcgacta gagaatgatg gtgctgatat gattggtatg acaggtatgc
     4501 ctgaagccat tttagccaaa gaactcgatc taccctatgc tgcaattgct gcagttgcaa
     4561 accatgcagc tggtcgcgga accagtgcgc gtgcaattcc ccttgaagaa gtttatgctg
     4621 tactggaaaa ggcaatgatt agtgtccgta atattctaga acatatggta attgaacatg
     4681 gcgattaaaa ctgttttaag aatgggtgat ccggtattac tccaggttgc caagaaagta
     4741 gagcaatttg atactccaga attgcaaacc ctcttgcaag acatgcatga tacgatggct
     4801 tcactcaatg gtgcaggctt agctgcccct caaattgggg tcagcttgca agtcgtcatt
     4861 tttggcgttg actataatcc acgttatcct gatgcagaaa ctgtccccta cacgattttg
     4921 atcaatccaa aaatcacacc gctatccaag aaaatggaag aagattggga agggtgctta
     4981 agcgttcctg ggatgcgcgg gaaagttccg cgctatacca aaatacgcta tcaaggtgtt
     5041 gatcaacatg gaaatcccat tgaccgaagt gttgaaggct ttcatgcgcg ggtagtgcag
     5101 cacgagtgtg atcacctcat tggaatttta tatccgatgc gaattagaga tttcagacaa
     5161 tttggtttta cagatgtctt atttccaggg caggaaataa tggatgacta acgaattgat
     5221 caccccaatc accaaggttt cactacaacg agaattacag cggcgattag aatcacaacg
     5281 ggaatttcat taaaccaccg ataatagaca tgactgtgtt gattccgatc atgtttgaaa
     5341 tccagtaaga tttttctgca atagatatga taggcaatca atactacgac cagcgcaagt
     5401 ttagcgtgta gccaagcgcc agcgaaaccg taccccagcc atagccatat tccaaaaaca
     5461 atcgtcaata aagcacccgg ggtcataatt ccatagaaca acttgcgttc catgatttta
     5521 aatcgatcat tacctgcctg atcagtgcac atagcatgat aaacaaacaa gcgtggcaag
     5581 tagaataatc cagcaaacca ggtgaccatg aaaatgatat gaaacgattt tatccaaagc
     5641 attgtacttt cttcccaaga atgtttttaa tttaagtttt aaataaagat tataacgggt
     5701 gtagcttaca attgaatttc cgattgctct ctttcttgtt gttattttat ggtacttcag
     5761 taatagcgag taatacttac ttagaagacc cagatagcta ttacgcacga ttgaaacaat
     5821 tacaacctgg cgatcatttg cagctaaaac caggcgtcta ccgaaaagga ttacctattc
     5881 atcacttacc aggcaatcgt cttgcgccaa tcacaatttc tgggcctagc ggtcaacaat
     5941 cggctatttt tatagggcga ccgggacata atacggtaag tattattgac tcaagtcata
     6001 tcaccattcg taatctcgtc attgatggaa ttgggttacc cgttgatgga atcaagtgtg
     6061 aaggtcatgc aaattgggca catcacatta ccttggaaaa tttacagatt tatggccacg
     6121 atcataatca agaaattgtg ggaatctcaa ctaaatgccc cgcctggaat tggattattc
     6181 gccataatat tattaagaac gcgggaacag gaatttatct tggaaattcc gatggtagag
     6241 caccttttat cgctggtctg attgaataca atttgatcac ccataccatg ggctataacc
     6301 tccaaataaa acatcaactc cctcgcccga gtattcctga tattcctatc gagaaacaac
     6361 acaccatcat ccgacataat gttttcagca aagctgatca atcaagtacg ggaaaagcag
     6421 cgcgacctaa tttactggtt ggtcactggc ctactgacgg caatgggaaa gatgatcaat
     6481 atctcatcta tgggaacttc ttatatcaga atgctactga atcattattc caaggtgagg
     6541 gcagaatcgc cttctataat aacctactgg taaaccatca aggcaatgca attcggttac
     6601 aaccccgctc agcgcaatcc gatcatatca gtatattctt taacaccata ctgacctccg
     6661 aaattgatat ttttttagct aaacccaaga ataaatcaaa ttcgacctat tggataacgc
     6721 ataacgccat ttttactgga aaatcggcta ctgcatcaag tctgaccaaa aacaacttag
     6781 tttatgatta tccattagca ggacactatc ttatccaacc ttacttaatg ccaggccaga
     6841 tgaatttaaa tctcattcct caggatagaa tggttcccaa actcaatatc aatcatttaa
     6901 aaaaccacct tgactggaat gtggattttg atggggaaat cagtgaacaa cgttttggtg
     6961 cttatacaaa tgaaaaaaga tctcgctggt atcccaactt ggaaatcaaa ccagttttaa
     7021 caacgaagca aaaaaataac ttaaccacac actaagccaa tcaattcata tgaaaactct
     7081 gctggttatc actttattga tcatttacgg atcactgtat ccgggggatt tcttgacacc
     7141 tgagcccgat gctctcgaaa aatttttcac agatttgcgt tggacaacat ctctgagcga
     7201 tatgctggga aacgtgatgc tcttcgttcc ctttggtcta gttagtagct tagtgactgt
     7261 tacacattat cactctcgca cacgattagt cctattcttt ttttctggtt ttatcctcgc
     7321 tttcgctctg caaattgcgc aagtctggct accgtcgcgt tcagccattc tcgctgatgt
     7381 tatttggaat ctggtaggcc tatgcatagg aatcatcatt gcacattttg taaaaaaatt
     7441 tacacaaaca agaatccatg ccttagacac taagattatt atccccttag ccactctcat
     7501 cctctggatg atcagtgaat tggtaccact tgtgccagcc attgatctac agaaatttaa
     7561 ggatgcactt aaaccccttt ttctcgaatt tgatcttgat ttccctaccg cgctactcca
     7621 tatggcagga attttattag cagccagtgt tttaaaagtg gttactcatt cgccactacg
     7681 ttggctaaca cttatcttgt taacagtgat cgtcggtaaa attactatca ttagcctaca
     7741 actcgatatc actacgctag tcggtttaac agccggctat ttattcagta tcgccctgct
     7801 atatcttgaa acatccaaac tcttagctat aagcttctgg gtacttttat tagcaggtac
     7861 tttagaacgc ataacccctt ttgtgcttgc ttccgatggc acacttagct taattccttt
     7921 tgccactatg ttaaaaggct ctatgcaaac cagtgcagtg gggttatctc aatcgctgtt
     7981 catctatact gctttactct ggctaaccca gaaaatgggc ggaaacttta aaggaattac
     8041 aattgcggtt gttatttgga cagtcttcat agaacttact caaatgacgt tacttggacg
     8101 cactgcggat attactgagc cgttattggt catcttcatt tcctgggtat tcgctgagtt
     8161 ccagcactat accaataaaa ctcaaaaaca atcgattcct gacaagccat atactgattc
     8221 gagttactcc tcaccccaag ctatcagtac aatccctgtt cagggaaatg gctttttgct
     8281 ggtatcgatc atcctgatgt taggaataac cggtgcgatt tggtttatcc tacgcttgcc
     8341 aggtatccct tacaacctta aagagttatt ccttggaaat gggaacctgt tgtttatctt
     8401 tatttttgtt caagcgcttt tctggattgg cactggagca gcttgggtaa gtcacaaggt
     8461 agccacgagt atcaggcctt acctaatatt accatgctgg actatgttgg ttagccttat
     8521 tagtcttatc ttactctcta ttagtgtgac acaagaaagt atcgccgaca tcgccggctc
     8581 caataatctt tattggtttg tgatgaaccg agatatctgg ggagaaaatt ggaaacctat
     8641 ttttgaatgg attggccctg atacggtctc gattctcgaa cgaatcgtta gatatactgc
     8701 tttatattct cctctcatta tttttttaat tttgttcaca ttgcctatgt ttgagcaaaa
     8761 aaaattaacc attcaacaat caacccttct tatcatcgtt acattactct ggctctggct
     8821 ggctaaagct atcgcctttg actggtcttc aacagataat ctgaatgaat tgattgctcg
     8881 agatggtccc atgggatggg gaggtggagg ttatctctat gccttgttgt tggtgctttg
     8941 ctttaacgcc acactactcg caaaaaccgt taattttcga ttaagcagta tattctcgtt
     9001 tgcactcact attgccgcat tacccctctg ttggtggcta ttaaccaacg gtttagaagc
     9061 taacgttgaa aaatatggaa ataccttctc aggaactcag tttttactag gaccagatcg
     9121 gaaacaagcg cttcctgaaa ttgaactgtt tattcgttgg agtatcgttc aaattggctt
     9181 tgtgacggta attggttttg gcattcgcaa ctattatcaa attaaatcac tggcttctaa
     9241 gataaaatga gcgaaattgc atccttaatt caattctatc tttcagctta atatgaatta
     9301 gaattcacgc aatagtccaa gctgttatta attcctttta cttacaggag aaagtaatga
     9361 gtaagccttt tataatattc ttatggttaa cagtagcact gttctttttt tcatctcaag
     9421 cggtaatggc acagggttac tcaaccatgg gcagcggagg tagaacaggg caaagctaca
     9481 tcgccatgtc gggcgagaag ctggcgactg gtgtcgttaa tatcgcaacc gggattgtag
     9541 agttacctaa aaccgttatc ttaaccagtc aacgcgatgg agcaccctac ggtttaaccg
     9601 ttggccttat cactggcatt atgcatagcg ttggccgcac tgtattcggc gcattggatg
     9661 ttgtgacttt tctgattcca acccctccaa ccgttcgtcc aaaatacatt tgggaaaact
     9721 ttgataaaga aacgacctat ggttaattag tggctcgatc agtgtggatc aataagcatt
     9781 tatcaatcgc attcatgcat attgatccac ttttaaaatg acccgtctgc actttacgtt
     9841 tagcaagaaa catacactgg atttcttcca tcaaaaaaat aaaacatcct agatgtagct
     9901 aataagcgat gaagcatgat cttcactcaa gataaatccg agaaacgtac acacgaaaga
     9961 gtaaccctgc gaactcaagc aacgatcctt ttgtcggaca gaaaagcaat aaaggttcgt
    10021 acgcttgata ttgcattagg tggcatggca attgtggcgg atggtaatcc taactctggc
    10081 gttgttttta atattcaact tacgctccca ctgcatacag atattcctcc tctatttgaa
    10141 gcaaaagtaa gggtagcgca tagtgtctta tcaggtaaag aaggaggatt caagattggc
    10201 ttacaattta tcgacctcaa tgatgctgcc gagtcaatgc tattgcagtt tctggagcaa
    10261 aattcaaagt aaaggtgcct cttaacggta tttccaacca gattggaata gccatccaat
    10321 catcggcact aatcaaagca cattactaac aaacagcact ttttttgtta tgcgctatga
    10381 tcacaaaatt tcatttcaga caaattgcac agaaacaagt taaaattggc agcaatgcaa
    10441 tagtcgatga ctcgtgattt accaacctaa ttacaaataa atgcgttttc tacgcctcct
    10501 aaaaatctta ttgattgccc tccgcttcgg cttagatgat attttactaa gccatgcaaa
    10561 acttaagatt ttaagacctc ttacactgct gaatcctttt cgtacacgtt taaagttgcc
    10621 gcgtgctgta cggcttcgtt tggcactcga gaagctaggg ccgattttca tcaagttcgg
    10681 tcaaatgctt tcaacacggc gtgatctctt agcgcaagat tttgctgatg agttagctaa
    10741 attacaagat caggtccctc cttttccttc tgatttggca atcgccacat tagagcgtgt
    10801 ttatgaaaaa cctgtcaatg aagtattttt acaatttgag cgacaaccca ttgctagcgc
    10861 ttcggttgct caagttcacc ttgccgttct gcacaatgga accgaggtag cagtcaaaat
    10921 tctaaggccc gctattgcac cgattatttc gcatgatgtt gctttgatgg aaacaaccgc
    10981 ttggctatta gaatcaattt ggccggatgg aaaacgccta aaattacgtg aagtcgtgtc
    11041 ggaatttgca agacaccttg aagatgaact tgatctcgtt cgtgaagcgt ctaactgtag
    11101 tcagcttcgt cgcaattttc tcgattcacc tttattattc gttccagaag tctattggga
    11161 ttattgttat cctgaagtaa tggtaatgga acgggttaaa ggaattccaa ttagtcatgt
    11221 tgataccttg cgtgaacata atatcgatat ccctcaactt gctcgtatgg gcgttgaaat
    11281 attctttaca caagtattcc gagatggtta tttccatgct gatatgcacc ctggtaatat
    11341 ctttgtaggt tcggatgggc gttatattgc aatcgatttt ggaattatgg gatcacttag
    11401 cgatgaggat aaaaactact tagcgcaaaa tttcttaggc ttctttcagc gtgactatcg
    11461 gcgggttgcg caaacccatg tcgatgccgg atgggccccc aggaataccc gtgttgacga
    11521 cttcgaagct gctatccgtg ctgtctgtga gcctattttt gacaaaccac tcaaagaaat
    11581 ttattttgga agaatcttat tgcgcctatt ccaggcatct cgccaattta atgtagaaat
    11641 tcaacctcaa ctcgttttgt tacaaaaaac actactcaac attgaaggtc tggggcgaga
    11701 tcttgatcct gatcttgatt tatggaaaac agccaagcct ttcttggaaa actggatgtc
    11761 agagcaaatt ggttggcgcg gtctgctcac ccatttacaa aaagaagccc ctaattgggc
    11821 cacaattctt cctgaatttc cccgactcat tcattaccac ctgagtcaag aaagggcaca
    11881 agtactggag gatcgactca ccgacctagt tacacaagaa aaaaggcaaa gccgcttatt
    11941 agctttgctc gccacattac ttgctgcact gttactggta caaatatact tataagttat
    12001 ctactttagc aatctagaac gattcttttg cctaattttt atcacgctaa tgagttatta
    12061 tcaaaaacat atcttttttt gcgtcaatca acgtgaaaat agtgagcgtt gttgcaacga
    12121 tcatcatgca caagtcatgc gtgattatgc gaaagaccgt ataaaaacgc ttaaactaag
    12181 tggaaaaggt aaaattcgtg tcaataacgc ggggtgtctt gatcgctgta atgaaggtcc
    12241 tgtcatcgtc atctaccctg ataacatctg gtatacctat gtggatcaag aagatattga
    12301 cgaaatcatt gaagaacatt taatcaatga ccgtatcgtc gatcgcctca aaatttagtt
    12361 tgcaaaaaag ccacttgatc gcaagccaat ttgaaaaacg cagttgagca aaaattattt
    12421 gttatcggcc cagccggcaa gatagaaacg atcattacgt ttccaattga taaacctagc
    12481 ggcatcgcca ttatcgcaca cccacatccc ctgtatcagg gaaatatgga taataaggtg
    12541 gtttacatca ccaacaaagc gctgatcgag aaaggattca tcactgctaa atttaatttt
    12601 cgaggcgttg gtgcaagtga aggaaagtat gcggaaggag tgggggaaat tagcgatgta
    12661 ttagcagtga cccgattcat gcaagaaaat tacggggcaa cactcaatca cgctttattg
    12721 gtcttagcag gcttctcatt tggtggtgcg attcaagcct ttgccgcaca gcagctcaat
    12781 ccgcacaaac tgattctaat cgcaccttct gttgcacgca tgaatacacc acaagtggct
    12841 gatcatgccg gagcaacctt aatcatccat ggcgatcacg atgatatcgt accgctgcaa
    12901 tccgttatcg attgggcgaa cccccaaggc attgcagtca ccgtcattcc agatgcagca
    12961 catttttttc acggaaaact caccattctt aaacaaacca tcatgaattt ctgttaaatc
    13021 taaataatag tttccatcaa ctcatcaatg cgggagcttc aatcagcaaa aacgctttaa
    13081 atgtttttgc cacgttagaa aaccgcttgt gtttacggtg cacaatatac cagtgacgaa
    13141 tgatgggaaa tccctcaaca ctcaatattt tgaggcgatg agtttcaagc tctaactgta
    13201 tagtatgcaa ggacattacc cccagaccca taccagcttg aactgcttgt ttgatggctt
    13261 cagtggtatc agcttccatg cctttattaa ttctaatgcc ttgctcaaca aaaaaacgct
    13321 ccattgcatt gcgtgttccc gatcccaact ctctaacaag aaaaacttct tcctcaagcc
    13381 gtttaaccgg gatattttgt tgactacata aaggatgctg tggtggggct acaatgacga
    13441 gcgggttttc catgaaagat tcagcggtaa tatcgatccc gtccggaggt tgtcccataa
    13501 tggccagatc ggttaaatta tcagccaaat gcttaagtac tgtttcccgg ttagatacgt
    13561 gcaaactaac cgttacccct ttataacgct gacaaaattt tgctaacaag tttggcgcaa
    13621 aataattagc tgtactcacc accgcgatat ttaattttcc gcgctccaat cctttcaatt
    13681 catctagcgc caattccata tctgacaact gttgagcaat cgcacggcta tattgatata
    13741 gttcttgacc tgcttcagtc aaatggatgc gtttcccaat ttgctcaaat agtggtagcg
    13801 acacaacttc ttccagttgc ttgatttgca atgaaacggc aggctgcgtt aaaaagagct
    13861 cctctgcggc cttagagaaa ctcagatgac gtgcaaccga ttcgaatact tttaattgac
    13921 gcaaggtaac atgcaacata gactaaatcc tgagataaga tcggtttatg tataagtaaa
    13981 atcttatcat atccatcaga attattaatt tgttcttatg cttatctctc gctatagtta
    14041 tccctgaatt gtatgtaatt caacattaac aacgtttcac cctatacctt ctcaagttct
    14101 ttgtagcttt ggcaggattc tcactaagga gactttacga tggcagtaaa aacctacaac
    14161 gctggtgtta aagagtaccg gcaaacctat tggatgcctg aatatacccc gcttgatacc
    14221 gatattctgg catgtttcaa aattacccca caacctggtg ttgatcgtga agaagccgct
    14281 gcagcagttg cagcagagtc ttctactgga acatggacca ccgtttggac tgatttattg
    14341 accgatctcg attactacaa aggtcgtgct tatcgtattg aagatgtacc cggtgatgat
    14401 acttgcttct atgcatttat tgcctatccg atcgatttgt tcgaagaagg ctccgttgtc
    14461 aacgtgttta cctccctcgt tggtaatgtt ttcggcttca aagcaatccg tggcttacgc
    14521 ttggaagacg tgcgcttccc tatcgcttat gtgaaaacct gtggtggccc tccgcatggc
    14581 attcaagttg aaagagatat gctcaataag tatggtcggg cattgcttgg ctgtaccatt
    14641 aaacccaaac tgggtctctc ggctaaaaac tatggtcgtg ctgtgtatga gtgcttacga
    14701 ggtggtcttg atctcactaa agatgatgaa aacgtcaata gccaaccctt catgcgctgg
    14761 cgtcaacgtt ttgactttgt gatggaagca attcacaaag cagaacgtga aacaggggaa
    14821 cggaagggcc attatctaaa tgtaactgca ccaaccccag aagagatgta caaacgtgcc
    14881 gaatacgcaa aagaaatcgg tgcaccgatc attatgcatg actacatcac aggcggtttc
    14941 tgtgccaata cggggctagc aaactggtgt cgtgataacg gtatcctact ccatatccat
    15001 cgtgcaatgc acgcggtgat cgaccgcaat ccgcaccatg gtatccattt ccgcgtactg
    15061 gcgaagattc tacgtctatc tggcggtgat catctgcact ccggtaccgt agtgggcaaa
    15121 ttagaaggtg atcgcggtgc aaccctcggt tggattgact tgatgcgcga aagcttcatt
    15181 aaggaagatc gcagtcgtgg cattatgttt gatcaagatt ggggttcaat gccaggcgta
    15241 ttcccagttg catcaggtgg gattcatgta tggcacatgc ctgcattagt tgcaattttt
    15301 ggtgatgatg catgcttaca attcggtggt ggtacattag gccatccttg gggtaatgca
    15361 gcgggtgcgg cggctaaccg tgttgcgttg gaagcttgtg ttgaagcacg taaccaaggt
    15421 atacaaattg aaaaagaagg aaaagctatc cttaccaatg ctgctaaaca tagccctgaa
    15481 cttaaaatcg cgatggaaac atggaaagaa attaagttcg aatttgatac cgttgacaag
    15541 ttagacgtcg ctcataagta acattgattg attcctctga actttaaaat tcagaggaat
    15601 accaaaccat accaggagtt ttaataatgt cagaaatgat tgattacaaa agtcgattaa
    15661 gcgatcctgc tagtcgcaaa tttgaaacct tctcctatct gcctgcaatg gacaaggaac
    15721 aaattaagaa acaagttcaa tatatcgtga gcaaaggctg gaatcctgca gttgaacata
    15781 ccgaacctga gtatttgatg agtaattatt ggtatatgtg gaaactgcct atgtttggtg
    15841 aaaccgatgt tgatcggatt ctcaaagaaa ctgaagcttg ccataaagcc aatccaaaca
    15901 atcatattcg tctcgttggg tacgataatt ttgcgcaaag ccaaggaaca tccatggtga
    15961 tttatcgcgg aaaaacagtt taatacgcag cacgggatta tccgtcttac aacccccgct
    16021 tgctgcaaag caacgggggt ttttatttcg tttcaaatga tttagtacga ttttaccaag
    16081 cgttatccgt tggagagaaa aatgagtgaa gccataaagc aatatttaat caaagatcaa
    16141 ccttactatc accccgttgc agacgaagtg gccctctatg aagctgccta ctcagttagg
    16201 atgccgatga tgctcaaggg accaacgggt tgtggtaaaa cccgattcgt agaatatatg
    16261 gcatggaagc tgaaaaaacc gctgattacc gttgcttgca atgaggacat gaccgcttcc
    16321 gatttagttg ggagattctt actcgatgca caaggtacgc gttggcaaga tgggccgcta
    16381 acgacagcag cacgtcatgg cgccatctgt tatttggatg aagtcgttga ggccagacaa
    16441 gacacaaccg tcgtcatcca cccgctgaca gacaatcgcc gtattttacc gctggaaaaa
    16501 aagggggagt tgattcatgc gcacactgat ttccagcttg tcatctctta taatcctggc
    16561 tatcaaagcc tcatgaaaga cttaaaacaa tcgaccaaac aacgctttgg tgcgttggac
    16621 tttaactacc ccgatcatga ttcagaaacc gagattgttg cccatgaaac cggtattgat
    16681 aaaaaaatag cggacaaact cgtttccatt gcagaacggg cacgtaatct aaaaggtcac
    16741 ggattagatg aaggcatatc aactcgtatg ctcatctacg caggaagtct tattgcaaaa
    16801 aatgtagatg cgcatgctgc gtgtcgagtc gccctagtac ggcctatcac agatgatccc
    16861 gatatgcgtg atgcgctcga tgctgcggtt tctactttct tctaaattgt tttttatcga
    16921 taacaatcat cgttaaatca aatgagcatt aaccttagtg attacacaga actgctagag
    16981 tcgctaccgc ctgaaaccat ggcgttattg gaaaacactt ggcacgatgc aacccgtgta
    17041 ttctcgccgc gagggcttga taattacatt aaaggtacat cagcgttgca aggattgggc
    17101 aaagggcgct cccttgtcga agcctggatt gacgaagcac cacaagttgc taaagaaatt
    17161 ggcgaagatg tcatcggtga tttagcaact acgctactca tgctcgcttc taagacatcg
    17221 ggcgccgtta tcgagttgat catgtcaaca tcgacgacag ccgccaaacg attaggtgat
    17281 gcggatttat ttcgtaacta cttgcaattc ctcaatacac ttttggcaca ggcgccacgt
    17341 ggtgttcgtc caatgctcga caaattggac gtcctatttg gtcaactcac actaggcggt
    17401 ctgagacgtt gggccttgtg gggagctcat gcccatcgga ctaactacga agagcaaatc
    17461 aattatttca gcttgagttc aaaagaatcc ctcgctgttc ttcaaagaga gcggaaaggg
    17521 actttatttg ttgacattca acgtcgaatt aatatttatt tacgtgcatt gtgggcacgt
    17581 gattttttta tgcgccccac ttctggggat tttgaaacgc gccaaggtta tcgaccttat
    17641 atcgagaatt atttgattca tttgcccgat gcttatgatg cgcatggcga aattgctgca
    17701 agtgaagtct atcgtgcagc cgcagcccat gctgctgctc atttggtcga gaccaaatac
    17761 ccaatttctg ctgaaagcct taatccatta caaatggctg ttatttcggt tatcgaagat
    17821 gcgcgcgtcg aggcactctc gattaagcgt tttcctgggc tgcgtcagat atgggctaaa
    17881 ctccatgatg caaaccctga tatggcaaca acgattgggg attatctgaa tcgcttagca
    17941 cgagcactgc ttgatcctga ttaccaagat cctgatgagt ggatcgtcga aggtcgacta
    18001 ctctttgcgc aagcgcaaga tcgcctcagc gaaaatcaac tttcctggga aattggtgtg
    18061 cagcttgcgc ataaaattat gggtaaaaag attccattta accctcgaac cgatgttctg
    18121 acagcaccct acttagacga taatcgctat ttctgggaat ttgaagaatt caattttgaa
    18181 cgctcgcaaa cagctggata cgaagagccc aaacaaattc gtaaatacgt taatgtgatg
    18241 gagcttgcca acgaaatcga tgttgaaaca gcgggtgacg atgcccaaga aatttgggtt
    18301 gtcggcactg aattctttcc ctatgaagat atgggtgtct ctttcaatga aatggaaggt
    18361 aaagagcccg tttcaccgcc gtttcattat gctgagtggg attaccaaat tcagcttgag
    18421 cgccccactt gggtcaccgt acaggagaaa cgcgcaaaag caggggatat tgtgctcgtc
    18481 gatagcattg caacacaata caaacgtgaa atttctcgca tgaaattttt attggacgca
    18541 atgcaaccac aaggggtgca acgcattcgg aaactcgaag atggggatga aatcgacatt
    18601 aatgctgcga tttcatcaac tatcgatatc cgcatggggc aacagccaga tccgcgcatt
    18661 atgatgcgct cggttcgtaa gatacgcgat atatctgtgc ttgtcttgct tgatttatcc
    18721 gaatcaacca atgaaaccgt ttcaggccag gaattttcgg tgctcgatct gacacgacaa
    18781 gcaaccgtgc tgttagctga tgcaattaat aaaattggtg atccctttgc tatccatgga
    18841 ttttgctcag atggtcggca cgatgtggaa tattttcgct acaaagattt caatcaaccc
    18901 tacgacgagc agcctaaagc caaattggcc ggcatgacag gccaactatc gactagaatg
    18961 ggagccgcga tccgtcatgc gagccaccac ctaaagcaac aacgctccag taaaaaatta
    19021 ctactgatca ttactgatgg agaacctgct gatgtcgatg ttagagatcc ccaatatttg
    19081 cgctacgata ccaaaaaagc agttgaagaa gcaggcagat cgggtgccat tactttctgt
    19141 atgagtttgg atcctcgggc cgatcagtac gtttcgcgaa tttttggcgc taaaaattat
    19201 atggtcgttg atcatgttga gcggcttcca gaaaaattac ctttgctgta tgccgggctt
    19261 acccgataat tagtcacact acgaaattaa accttggaat cattgcgagt atgtcacaac
    19321 aagtctatgc aggaattgat ttagatgaaa atggaggact tacgcattta gggcgtattg
    19381 tcttagacgc ctgggttttt ggtattctac ccgaaacaga aacctgtact ggttggagtc
    19441 atgctcaaat gcagaactta tatgaagaag tctatgctgc ttgggaaccc tttgcacacc
    19501 tacctagccg ccttccacct gaattaagcg caaaacatga gaaactttat ggccaagcga
    19561 ttacgattgc caaacgtaaa ggctggtcag cagaacttgg ggaagacgat taatatcaac
    19621 taaccttaag aatcaatctc atctacgcac tctcgaccat taagtattga tcactggaaa
    19681 cggaatgatt tgtgcaggcg atttgatagc cgctttattt gcgagaattt gcagtgcact
    19741 cggaaattcc ttttgaagcc ggagcatcag ttcttcttga ttctctcgat agtaaacaac
    19801 ttcgtaagcc actggttgat ctaaggaagt acgattttgg gtttggaaat tctgccaggc
    19861 aatgtcaacc caacattttc gctcgccatc gataaaaaca aattcctttt tatcgagaat
    19921 agctaaatac tgcatagcgc gaatcggcac gaataaatga tccgtggtac accgtgtgat
    19981 tagctgacat gctaaattat aagtcgtagc ggggagaaga cgcacctcac gtttaatttc
    20041 ttggctacgg taacaagtaa tatccatcat tctttctccc tatattcatt tacattaaat
    20101 ctaatgcgcg cgactcgcct aaataaacct cctgccaatc ggaatgattg gaaaacaatt
    20161 cgctccctat taccctattt atgggaattt aaaggccgtg ttctcattgc cttaatgtta
    20221 ctgctactcg caaaacttgc caatgttagc gtaccattga tccttaaaga aatcatcgat
    20281 gcgctagacc ctgcacaaac gatagtaacg ctaccacttt ttttactgat tgcttatggt
    20341 gtcttacgat tctgcagtac cttatttggc gaattacgtg acgccatttt tgcgaaagta
    20401 acacaacgcg caattcgacg tgtcgcaatt cgagttttca gtcatttaca ttcactatcg
    20461 cttcgttttc atttaacaag acaaacaggc ggtgtatccc gtgatatcga acgtggttcg
    20521 cgtggtatct cgtttttatt gaatttcatg ctcttcaata ttctaccgac gttacttgaa
    20581 atcagtctgg tcatggtcat actcatttct aaatatgata tttggttttt tatcataact
    20641 tttataacgt tactttctta catcgcgttg actgtttttg tcaccgaatg gcggatgatt
    20701 tttcgccgca ccatgaacga tatggactca aaagccaata cccgcgcgat tgatagcctg
    20761 ttgaattacg aaaccgtcaa atacttcaat aacgaagctt gggaaactca gcgctacgat
    20821 gaacacctac aacaatggga aaaagccgct gttcgtaatc aaacctcgct agctaccctc
    20881 aacacgggtc aaagcgcaat tatcgcaatc ggcgttactc tgctcatgat gttggctgcg
    20941 agtaaagtag tttctggcga aatgacctta ggtgatttag tgttagtcaa cgcatttctc
    21001 ttacaactct atatgccact ccatttctta gggtttgttt accgtgaaat caagcattct
    21061 ttagctgaca tggaaagaat gtttacgcta ttagctgaga atgaagaaat acaagatcaa
    21121 cctaatgcaa tcgcattaaa tatttctcac gcctcaatca catttaaaaa tgtcaatttt
    21181 agttatgaat ccaaccgcca aattcttttt gatgtcaact ttagcattcc ttccggcagc
    21241 acactggccg tcgttggtca tagtggctct ggaaaatcca cattagcacg cttattgttt
    21301 cgtttttacg atacatcgag cggtgcaatt ctaatcaatg gccatgacat caaacagatc
    21361 acacaaaaaa gtttacgctc gatgattggc atcgttcccc aagacaccgt gctgtttaat
    21421 gaaagcattt attacaatat cgcttatgga agacccgacg catcagaagc agaaattcta
    21481 gcagccgcta aatcagccca tatccatgat tttgttcaaa gccttcccaa tcaatacgac
    21541 tctcaagtcg gtgaaagagg attaaagcta tcaggtggcg aaaaacaacg tatcgccatt
    21601 gcccgagcca tcctcaagaa cccattgatt ctaatttttg atgaggcaac atccgcacta
    21661 gattctagaa ctgagaaaaa tattcagcac gaactcaaac aattatccaa aaatcgaact
    21721 acgcttatca tagcgcatcg cctttcgacg attattgatg ctgatcaaat tttagtgatg
    21781 gataaagggc gcatcgttga gcaaggaaac catcaacaac tcctcgctac caatggaatt
    21841 tacgctcagt tatgggaaat acagcaaagg gaagaagata caacgggatc cattacgatt
    21901 tgattaatgc tcatttcgct cgcatcaact atgagcttta tcacgattcc ttcacaaatc
    21961 acaccgtaca atcttagcca tgtaatttat ttcattcaca ttaacgttag agcaatgtga
    22021 atgaataatt tttcttgaca tctgaataag gtttttttat cgtcagttaa atttgaaaaa
    22081 taacatcact tataggggaa gaaatatgct tgatagtata aagaagatgt tacagcagct
    22141 aatgggaagt agcagtgaag attcaaatca agcaacagat ataacatttt atacactcgg
    22201 caaatttaat ttgggtgtcg gtaaaattgg acctgataat cggacagata tcgaccaaag
    22261 cgtattaaaa gaaatgtacc gccgtgtagc agctggcgaa tttaatatcc ctgtttcagg
    22321 gaccttacca gaaatttgtg ttgatggccg aaccaatcga caaggttcac gaatcaatgc
    22381 gccttctgca gcaggtggaa gtttaacgat ggtgtatggc cgagatcttg gtgactctgg
    22441 cgcattaaat gataaaaccg aaatgcaatt aaccattgaa accatggata ccctcaaagg
    22501 aaaagggtat acaaccgcag tgcatggcga tgatcatagc aactgtgggt gcggtgcctg
    22561 tgctcatgca caaaaagttt atcaacatat tactgataaa atcgatccga tcgctaattt
    22621 aactggaaat tatggcattg taatcagcga cactgaaaaa gcgtccatta tgggtaaagc
    22681 aaaaaatcgt ctgaatcatc caagcttttt tgctgaagat cgatccgcca tcatacaggc
    22741 ctgtcaggat catggtgcga attatgagga actcgttgat gctcataatg agctgggtat
    22801 cgcaatcaat atgcgacaag gtactacgat agatcgtaaa gcgattagaa caactttcgg
    22861 acctcaatac gatctttttg tggttgatgc gtggactttt gcaactgccg cccaggaatt
    22921 agcactagaa agtaataaac aagatgctga ccgaatcgcc aaagccatca ccatacaaaa
    22981 tgtagcgacc gcatctgttc ttgggcactc atctctaccc atcataccaa ttggctaatt
    23041 attggattat tggctcaaac ctcaatcttt tgctattgta ttcataaata ggtgccaaag
    23101 aggctagttg tgaattttct agcctcttgc atctgaaatc attctcctaa agtagcacac
    23161 acaatataat caatggacaa ttgagttttt aataagactt aatgtctaat gaaaataatg
    23221 gatccagttg gaaattagaa atgcagattt tgattattga agatgatttt gcgattgcaa
    23281 cgaacctgta tgattatctt gaatctcgag gatatggggt tgatgttgcc tatgacggaa
    23341 ttgcgggaca ttgcttagct gtcacccaac attttgatgt aatcttgcta gatctgggat
    23401 tacccaaact agatggatta acgatctgtc aaaaacttcg ccaggaatcg caaatcgata
    23461 cccccatttt aatgttgact gcgcgcgata cccttgatga taaattgaaa ggcttcgatc
    23521 atggtgctga tgattacatc gttaaaccct ttgcgctcaa agaagtagaa gcgcggattg
    23581 ttgcacttca taaacgccat agtggcaaag taacgagtcg tacgctggtc gtcggggatt
    23641 tattatttga ccctaaaacc atgtccatta cttttgctgg ctcagctgtc aaattaccgc
    23701 caaagtgtat ccgtttgctt gcgctcatga tgagtgaacc tggccgagtg tttggacgca
    23761 aagaacttga gtccgcagta tggggcaata ctcaagaaac cagtgatacc ctgcgcactc
    23821 atatgcatgt attaagacga tcattatctc aagcaggtgg ctatgatccc atcgaaacag
    23881 tgcatggact gggttatcac ttaatatctc gtgcgtaaac atcgattaag gcatggtcta
    23941 cggcaacggg tcgcactagc gcttgcaata ttttgtatct tttttgtcag tatctttgga
    24001 attagtcttt atgtcgtttc aggcgatatc gaagaagatc atatcgagca aattctcaga
    24061 atggaaatgg agcatctcat ttaccgctat gagcaacatc atgacataag gccacaaatc
    24121 ggctctaatc ttgaaggtta tatcattact tcgatcgccg atgaaaagaa acttcctagc
    24181 tatttgcaag gacttaattt cgactatcac accatatttc tccggcaaga agaaatccgg
    24241 gtagcagtca aacacatcaa cgaaatcaaa tttctagttg c
//
LOCUS       DAOQLJ010000016        23184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_753_length_23184_cov_5.915561, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000016 DAOQLJ010000000
VERSION     DAOQLJ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 23184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23184
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_753_length_23184_cov_5.915561"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /gene="aceE"
                     /locus_tag="PK522_02355"
     CDS             complement(<1..846)
                     /gene="aceE"
                     /locus_tag="PK522_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110996.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNC40608.1"
                     /translation="MNPHDIDPQETQEWLDALESVLINEGPERAHYLLERLVEKARRS
                     GAYLPYSANTAYINTIPSGKEERSLGDHAIEHRIRSYVRWNAMAMVLRANRDTNVGGH
                     IASFASAATLYDVGYNHFWHAPSSERGGDLIYVQGHSSPGIYAYAFLMGKLSTQQLDN
                     FRQETGGNGLSSYPHPWLMPDFWQFPTVSMGLGPLMAIYQARFMKYLGSRNLVETTGR
                     KVWAFMGDGEMDEPESLGAITLASRENLDNLIFVINCNLQRLDGPVRGNGKIIQELEA
                     AFRGAG"
     gene            complement(895..1752)
                     /gene="folD"
                     /locus_tag="PK522_02360"
     CDS             complement(895..1752)
                     /gene="folD"
                     /locus_tag="PK522_02360"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNC40609.1"
                     /translation="MSALIIDGKAIANKVRDALKQRTEKLAQLGLQPGLAVILVGDNP
                     ASGIYVRNKAKACKEAGIHSEIYQFPGDTSQMTVLEHIHTLNHDPKIHGILIQLPLPA
                     HFDTQKIIESIAIEKDVDGFHPCNIGALATGNARFYPCTPYGVMTLLKEFNLTIEGQH
                     AVVVGRSNIVGKPMAYMLLEKGATVTICTSKTQNLSQYTQSANILVVATGKPQMITAA
                     MVRPGSVIIDVGINRLGDGRLCGDVDFASVKEKVGYITPVPGGVGPMTITMLLSNTIE
                     AAERVQSAS"
     gene            complement(1785..2447)
                     /locus_tag="PK522_02365"
     CDS             complement(1785..2447)
                     /locus_tag="PK522_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40610.1"
                     /translation="MDNNQINQTKSEDETDSLNNCIQYARTILNEQLARIKRKKYDFA
                     PEFKFMTIQLYLVGVMWQFYEKQQQMAPEVAREKAFEALHSMMIKDGAKPKRAEKQIT
                     FIKEMAKLEDGDEALAIHIGYESQPDDESLSEVFDHYVDEPRVSGALWRSYDRGKKII
                     LLGGLLVGMAAVWFVTIFIPESSELAILATGLVAACLFILPTFLIGLLIYRSKIKQSK
                     HS"
     gene            2698..4455
                     /gene="argS"
                     /locus_tag="PK522_02370"
     CDS             2698..4455
                     /gene="argS"
                     /locus_tag="PK522_02370"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNC40611.1"
                     /translation="MTVPKQNNFKVHFTELLYQATEKILPPNCEIFLELARPKQVEHG
                     DYSSNIALQLARFLHKNPKEIAQSLIQLLPDSPYLEKAEVAGNGFINLFLKKSAKQQF
                     LLQVLQQGSFFGTTDIGKGNQIQVEFVSANPTGPLHVGHGRGAAYGASLANILRANGY
                     SVTREYYVNDAGRQMDILTLSTWLRYLELCGVSIPFPGNAYQGEYVIDMAKMIKKAHG
                     DRYVHEPHALIARVTPASESSISNEDEQLDELIQVAKKTLSEDYAYIHQFVLTEQLGD
                     CRNDLMEFGVEFDTWFSEQSLFDNGSIAKVIAMLEKNNMLYQQDSALWFRSTRFGDEK
                     DRVVQRENGQYTYFASDIAYHLNKFERGFSHIINVWGADHHGYIPRVKGAMQALSLDP
                     KKLEIALVQFAVLYRDGKKASMSTRSGEFVTLRELRKEVGNDAARFFYVLRKSDQHLD
                     FDLDLAKSQSNDNPVYYVQYAHARINSVITQWGEAVSILSNAPTDSLSNEAELTLIQK
                     LIDYPDTLEAAAKERAPHFLAFYLKDLASAFHSYYNSTRFLVPEQELKLARLALIAAI
                     QQVIANGLNLLGVTAPNEM"
     gene            4476..5105
                     /locus_tag="PK522_02375"
     CDS             4476..5105
                     /locus_tag="PK522_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNC40612.1"
                     /translation="MSRDYKSRGGASSKGGGSLILGIFIGYALGLVSAIGVWLYINQA
                     PSPFLTEDKTANRPKEELLSKPTQTEKSVVSNTNNNNSTEESKSRFDFYKILPGIDEP
                     DSGNVPNAKEIAKASPQAESVQRGANSYFLQIGSFKSAHEAEKVKAELAFLGMMAAIQ
                     PTESSDKGTWYRVRVGPFSDIHEIDQIRASLQENQIESNIIRLPTSNMP"
     gene            5160..5807
                     /locus_tag="PK522_02380"
     CDS             5160..5807
                     /locus_tag="PK522_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HNC40613.1"
                     /translation="MKKLTKLFILLLIGCVSSVGVSVASTEIVEGKDYTVLSTPQATD
                     DARSIEVIEFFWYGCPHCYDLHPHIDQWRKNAPKDVKFRYVPAIFRANWTVAAKTFYA
                     MEALGISKDLHDKVYDAIHAKKIDLAKESTLFEWVEKQGVDRKEFVNAYNSFSVQNMV
                     NRASQMTKQYKLTGVPALVVEGKYLTSGKMTGLPRDTILVLDALIEKARKEKSSQ"
     gene            5919..8219
                     /locus_tag="PK522_02385"
     CDS             5919..8219
                     /locus_tag="PK522_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNC40614.1"
                     /translation="MGVANKSMARKSSDNPLPPKLAKLLRESGCLVLGAIALYLVLIL
                     LSFNRADPGWSHSENLNQITNTGGKAGAWLSDLLLYLFGASAWWLVALFIAAIIWSYR
                     RIDIVSLFDPRSLIISLIGFVALLMASSGIEALRFNTLNIALPLAPGGILGETISQGF
                     SRLLGVTGASLSFFILFAIGFSLFSGISWVRIAEKIGETIELSCVTLWQSWLTWLDRR
                     AGKLSSLERSEHVDDLKKDLSTHPPLKIEIPDTSIPKSSRILKEKQTPLFSELPDSIL
                     PPLYLLDEPKVDVELLSNETLEFTSRLIERRLQEFGVEVKVVSAHPGPVITRYEIEPA
                     TGVKGNQIINLVKDLARALSVASIRVVETIPGKTTMGLEIPNPKRQIVRLHEILGSQA
                     YADTESPLTIALGKDISGRPIVSDLGKMPHALVAGTTGSGKSVAINAIILSLLYKASP
                     EQVRLILVDPKMLELSVYEGIPHLLTPVVTDMRDAASALNWSVAEMERRYKLMSALGV
                     RNLAGYNQKIQEAKKAGQPLVTPTHIVSDDATPLEEMPMIVVVIDELADLMMVMGKKV
                     EKLIARLAQKARAAGIHLLLATQRPSVDVITGLIKANIPTRIAFQVSSKIDSRTILDQ
                     MGAEALLGQGDMLYLPPGSGYPQRIHGAFVADHEVHKVVEYLKQQGEPRYIEAVLKAG
                     DEEGDFNGESSENNRPSGETDLLYDEAVAIVLKSRRASISMIQRQLRIGYNRAARLVE
                     DMERAGLVSAMQSNGNREVLAPDRGD"
     gene            8277..8891
                     /gene="lolA"
                     /locus_tag="PK522_02390"
     CDS             8277..8891
                     /gene="lolA"
                     /locus_tag="PK522_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HNC40615.1"
                     /translation="MIRFVIVVVLLFLSLAAQASAVEHLREFVQKTQTFKAVFHQKLL
                     DQNFQVVQEASGSMMFQRPGKFRWIYEKPYQQLIVGDGKRVWFYDQDLNQVTVRQLDL
                     AIGNSPAALLAGSSAIENDFNLAEIQIEDEIDWLEATPKHKETNFELIRMGFAKTGEL
                     REMILRDNFGQFTLLVFSEINFNPNLASELFQFDPPSGADVISD"
     gene            8884..10203
                     /locus_tag="PK522_02395"
     CDS             8884..10203
                     /locus_tag="PK522_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNC40616.1"
                     /translation="MTELDFSSRPPLSPPLAERLRPRSLEEVIGQTHLLGIGRPLRNA
                     FETGKPHSMILWGPPGSGKTTLARLMSHSFDAEFIAISAVLSGVKDIREAIDRAKSIY
                     QQSQRHTVLFVDEVHRFNKAQQDAFLPYVEQGLITFIGATTENPSFEVNRALLSRAQV
                     YTLAPLSQEELQQLFDRTHQSVLKTLNFSYEAKQLIIQFADGDARRLLNLLEQLSIAA
                     EAEAYNTIDTNEVRKALTRSQRNFDKGKEAFYDQISALHKSIRGSSPDAALYWMCRML
                     DGGVDPMYIARRLIRAAVEDIGLADPRALTMTLDACQAYERLGSPEGELALAQATLYL
                     ACAPKSNAAYLAYKKARSMIEHDTSHPVPLHLRNAPTQLMQEMGYGKQYHYPHDEPEA
                     YAAGENYFPETLSPVTFYYPTTRGLESKITEKLEKLAEMDRQHKKTS"
     gene            11282..12664
                     /locus_tag="PK522_02400"
     CDS             11282..12664
                     /locus_tag="PK522_02400"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNC40617.1"
                     /translation="MDTSQSISIWVFGIGEYGEETFRRFLHLLTQQHIAIQDYCTPCP
                     YSGIIYSNKVTPELLNKLYELSNHGQVQVLACCQEAISGEAIWQLMEAGASDVLVCTK
                     DNLIIHEITARLRRWESISSLLNSPIVQNALVGKSQTLQTILRQVIEVAHFTNVSVLV
                     LGESGTGKELLANLIHTLDNRSEKGELIILDCTTIVPELSGSEFFGHERGAYTGATSS
                     RDGVFALANGGTLFLDEVGELSLTMQTQLLRVIQEQTYKRVGGNAWQRTAFRLICATN
                     RDLATQVQRGKFRADLYYRIASYICHLPPLRERSEDILPLANYFLKKNCPNQDIPIID
                     KPVEEYLLRRQYPGNVRDLKQVIARMLCRYAGKGPITVGCIPPDERPNLSEEWKEWRD
                     MNFERSIRIALSQGMGLKTISKNTEETAIRIAVDEANGNLQRAAQRLGVTDRALQLRR
                     AAQRQENSEP"
     gene            complement(12734..13486)
                     /locus_tag="PK522_02405"
     CDS             complement(12734..13486)
                     /locus_tag="PK522_02405"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNC40618.1"
                     /translation="MMIIDCHCHAGSGDGFTGPWDTRAPLRDYLRRATVAGIQRSVLF
                     AAFHSDYAAANKEVAKIVASQPDRFYGFAFVHAARDRRRVHELIETAVRQYGFVGIKV
                     HRHDAPITREICEVARAFALPVLYDVVGEVSVCELLAQEYPDVNFIIPHLGSFADDWR
                     AQSAIIDHLVRHPNIYTDTAGVRRFDLLEQAVKRAGAGKILFGSDGPWLHPGIELAKI
                     KALRLSPQQEKLIMGVNLQNLIFPNQKNQAIA"
     gene            complement(13519..14946)
                     /locus_tag="PK522_02410"
     CDS             complement(13519..14946)
                     /locus_tag="PK522_02410"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNC40619.1"
                     /translation="MKPVKQLASPVDLYSPVIDYQQISRARFVEELKAFIHFPSVSAQ
                     PEHDQDTRRCAAWLANHLRKIGMEKVMIVTTQGHPVIYAEKCHIPGKPTVLIYGHYDV
                     QPADPLAEWQFPPFEPVIRGDHLYGRGASDDKGQLFTHVKALEVLLRSGQGLPVNIKC
                     LFEGEEEMGSPHLPAFVVVNQQMLAADCAVVSDTQIPSARQPAITYALRGSINFELEI
                     IGQNRELHSGVLGGAIRNPLQVLCEMIAQLHDRTGRINIPNFYDRVRKWDAQEWAYMK
                     KIEPSDQSILRDAGVGVGWGERGYSIYERTTIRPAITVTSIHGGYQGEGVKAAIPTRA
                     LAKLNFRLVPDQNLREIDQLFRGYLKQVTPPGIRLQIRTSAPSRPVLMDRKHPVVMAA
                     VHAYRHGFGTAPVFLRNGGTIPVVNLIQDILHIPVVLMGFGLPDDRIHGSNEKFYLPN
                     FFNGIKTSIRFLAEVGKWQRTSRVQ"
     gene            complement(15005..16270)
                     /locus_tag="PK522_02415"
     CDS             complement(15005..16270)
                     /locus_tag="PK522_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40620.1"
                     /translation="MKTPIHFPIESFAFNFKFGDREYDRFINTRRGFSWETEVSQSQR
                     LPSSSGVTACPKGLICLDHTHVPKQVDPVHPGRFITEVKSKLNVKNMNPGFIDGADNI
                     ITDRSNTGLQTCLVRLLTSQFHHYLSHDSLQKKSASWNDRVRVGLVDLSGTKLVQPDF
                     AGWGSTVAMYGASVPKILALHAAFQLRSDLRSLAVNNGIAAGKALELAAVNQWKAKGL
                     KSGFPDLIWLFDIRNKTGNLGTLDFSVKARKVFQGIMHNAEAGELIIRVGFPYMASVT
                     WQAGLFHPSRGGLWLTTSYGKGQWGNNPIKGVQSANVTALSAATYFTLLAQGRLVDDV
                     SSKEMQTWLRGGCMTKHFPQGLGLVASKCGVWSDYWHDCALIARGRIRYVVVVLSRTK
                     PSQFPKYTQLIRELDNLIVRNNQIPRVAC"
     gene            complement(16378..18060)
                     /locus_tag="PK522_02420"
     CDS             complement(16378..18060)
                     /locus_tag="PK522_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNC40621.1"
                     /translation="MNQEMNFETIPLELTPEMGGEILEVESERGIRRFAGPKSRNLQS
                     GIRSGLRSSRTKRPPIKRSRSALRGFRRPFGSARYEPIQIVDEPYSSEPEPPRSERTR
                     WVQDCLNHAMGTQLPVNGIMGSKTRSVIRNFQRQQGIRASGIVGPDTEEAIKSICTSN
                     TAALSANGEELENQTLGLPGAHLQSQLTWLPNNANPRGYTLDEAITEIGGGIYIAVDV
                     SGKMPKILKVGQASDFNTRLPKYRSRTVKRKIKGKIQHVLPDLRFYLARVSGCRGGSC
                     GVGGLDTQVERAISRLLFRTHRAAATRPQWHREKYTIATVLGPLTVENILPKPLIHLL
                     NKAYTATGTDVYNQPITTYPSIPASQSSLLLDPATNPFWEFSPHRDLYGELEYPFSEA
                     EELELAAELLSISNEEELDLFLGKLFKGIGRGLKKFGRFIGKKVLPALGKGLKAIGKV
                     ALPIVGKALGSFIPIPGVGTAIGGAIGTAVSKALELEFNGLPSEEVELEMARRFVRIA
                     ATAARQAALANPHASPEAIANTALVAAARMHVPNLPHRLGSGSPGLGGFTGR"
     gene            complement(18089..19204)
                     /locus_tag="PK522_02425"
     CDS             complement(18089..19204)
                     /locus_tag="PK522_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC40622.1"
                     /translation="MAVINFASALYLGMHHPSQLLQSWQQLTLGVPAALAEPPGAEEV
                     AGKLAQLQGSEQGVLLPSTLHLFWDLFGMLHQQPVALFLDDGAYAIARWGVEQFRAKG
                     GDVYIFRHHDPEALLAQIKLHAQRKKHPVVIADGFCPSCGHAAPINDYLEIVRRFGGL
                     LVLDDTQALGILGASPAWCPPYGEGGGGLLRWSGNFGPDIVVGSSLAKGLGVPVAVLA
                     GSKALVKQFKSASDTRIHGSPPSVAVIQAARHSLNVNQAHGDLLRHRLAQRVAQFRKG
                     LETIGWGAIGGWFPVQILRGIVGDNAVRVHNYLSQKGVQAVLSRPRNSGEAHLSFLIT
                     ARHTSEEIDQCIDLLNTLTRIISRVANRSGKPAYSGF"
     gene            complement(19210..21354)
                     /locus_tag="PK522_02430"
     CDS             complement(19210..21354)
                     /locus_tag="PK522_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40623.1"
                     /translation="MNIRSKKIQNCEAPCHGTLRVHLHDSGENKLPIRGADIEVLEWN
                     LGEWRSIETRTTDENGYATFKLLGGDAHRYGLAFKNAFGFSGEPVGCSDDELQEELIS
                     IDCDCTKRISLGAPVNFTQQLAMCGPRSEEQLCQDIVAGQPAKLRISHSAALGNPEIK
                     LSSNQGILSQPSIFEDGMDRVYEIAIDTTGMQGNYSINAAFKHPDGSSIATNLAGTAM
                     RNVQHIDARTTTSLTRTETSFTEDTAFWTGIRNSAEALSFNNYLIFMDWIFCGKQKPR
                     ELSQFESDRYDEKNRIWRGKLLKNRFLPFTDSDAYRVVKAATEAFVMVNCGVHRDDLQ
                     PFDAIKDEAYLNRRDLPIPPYGLNQAFKNDYLVGIDGNGDVLPYLAVIRRKLPDIPIK
                     IKPFEDAANNGTGKIDADECFGLLQEKLTNPCLLELIWSYWHEEGMLVQTTNAITRRF
                     QNVRGSEQDPLSNLEMDPLRPLNNVLWGYIQDEQHRLTVNRRNYEYDHHYGLRLEGKA
                     VQNFRPADTRSKFLEAFHHLLRLCTVFYKQDDDTTVKADAFPVLNALKEVHLILSQGA
                     HNQFGDLPSTARIEMLMQQWLLARPEFRELLPTRIMVAYPEPWMDRVDAMKKLQGWTD
                     TSVLHFRNLGMFGEQLLLSIRWGHWSDEFEPVKALNWARFFRPQAQGYIHAYRAATGV
                     DLSADPMVNTRVDSTLPSVLLQKRLASQQRAM"
     gene            complement(21430..22719)
                     /locus_tag="PK522_02435"
     CDS             complement(21430..22719)
                     /locus_tag="PK522_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40624.1"
                     /translation="MAAASYATWMLEQEARALLTRLKRVKPFALHETMLPAASLLPAA
                     QVGIEQYLMKGRRELRRLIHRYIAWLRGPFGRRAKPAHAQRLFSLLRLRFNAVLTQFD
                     LFNDALTQRSESEIGVWLSGLEIVSADALAIPGHYYEVPPVICYLDRGVGAAIRRTRT
                     RLPGGGENPVAVIRIPRERMVGSGIASSLIHEVGHQSAALLNLVNSLRPVLQGMQRGS
                     GAYSKAWSLWERWVSEIIADFWSIARIGIASTLGLMGVVSLPRAFVFRLNRDDPHPSP
                     WIRVKLSCAIGQALYPHPQWQRLAQLWEAYYPIDGLDTAQQLNYQALQATLPGFVALL
                     VNHRPASLHGRSLAEVLQVQNRQPHRLKALFLAWQQQPAKMYQVSPVLAFAVIGQARA
                     DGRMTPEQESALLGKLLTHWALRSTLAVSATCAMRSV"
     gene            complement(22726..>23184)
                     /locus_tag="PK522_02440"
     CDS             complement(22726..>23184)
                     /locus_tag="PK522_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40625.1"
                     /translation="VAGKFIKSPVGQQLGGLLKGAAKQALPMVGSAIGGYFGGGSGAK
                     IGSQVASTAGRIFGLELEGLSHEDQEYEAAKRFVQFAGTAAKNVALSPQAVNPKAVAQ
                     SAVAAAARQLAPGLVRNNPVVTSGPCSACGRGTMSGRWVRRGSKIILLGV"
BASE COUNT         6475 a         5543 c         4937 g         6229 t
ORIGIN      
        1 gccagcgccc ctaaatgcgg cttcaagctc ctggatgatt tttccatttc ccctaacagg
       61 gccatctagc cgctggagat tacaattaat gacaaaaatc aaattgtcga gattttcacg
      121 cgatgcgagg gtaatagcgc cgagcgactc tggctcatcc atttccccgt cacccataaa
      181 agcccaaact ttgcgccccg tcgtctcaac taaattccga cttcctagat atttcataaa
      241 acgggcctga taaatagcca taagtgggcc cagccccatt gataccgtag gaaattgcca
      301 aaaatccggc atcaaccaag gatgaggata tgacgaaagc ccatttcctc cggtttcttg
      361 acgaaaatta tcgagctgct gcgtcgataa cttccccatt agaaaagcat acgcataaat
      421 tccaggggat gaatggcctt ggacatagat taaatcaccc ccacgttcac tactcggcgc
      481 atgccaaaaa tgattataac caacatcata gagcgtagct gccgaagcga aacttgcgat
      541 gtgcccccca acattggtat ctcgatttgc gcgcaatacc atcgccatcg cattccaacg
      601 cacatatgag cgaatacgat gctcaattgc gtgatcgccc aatgaacgtt cttctttgcc
      661 agatgggatc gtattaatgt aagcagtatt ggcgctgtaa ggaaggtaag cacccgaacg
      721 tcgtgctttc tcaactaagc gttccaataa ataatgcgct cgttccggtc cttcattgat
      781 gagcaccgat tccagtgcat caagccactc ttgcgtttct tgcggatcaa tatcgtgtgg
      841 attcatatcg agtaactgta atgttcaatt aaaggataag caatatcctt gattttagct
      901 agcagattga acgcgctcgg cagcttcgat cgtgttactt aatagcatgg taatggtcat
      961 gggaccgaca ccccctggga caggggtaat atagcctact ttttctttca cggaagcaaa
     1021 atcaacatca ccacaaagac gaccatcacc tagccgattg attcctacat cgatgataac
     1081 cgatcccgga cgaaccattg cagcagtaat catttggggt tttcccgttg caaccactaa
     1141 tatattggca ctctgggtat attgggataa attttgtgtt ttggatgtgc aaatcgtaac
     1201 ggtcgcaccc ttctctagca gcatataagc catcggtttg ccaacgatat tacttcgacc
     1261 cactacaacc gcatgctgtc cttcgatagt taaattaaac tccttcaata aggtcattac
     1321 accataaggt gtgcacggat agaaccgcgc attacctgtc gctaatgcgc ctatattgca
     1381 aggatgaaac ccatctacat ctttctcgat cgcaatcgac tcgataattt tttgcgtatc
     1441 gaagtgagcg ggtaatggaa gttgtatcaa aataccatgt atcttgggat catgatttaa
     1501 ggtatgaata tgctcaagca cggtcatttg tgatgtatca cccggaaatt gataaatctc
     1561 agaatgaatc cctgcttctt tacaagcttt cgctttatta cgcacataaa taccggatgc
     1621 cggattatct ccaactaaga taaccgcaag cccaggctgc aaccctaatt gggcaagttt
     1681 ctcggtacgt tgcttgagtg catcccgaac tttattggca atggctttac catcgatgat
     1741 taatgcactc ataatttttt aagacaaccc cccgcttatt gtctttaaga atgtttactt
     1801 tgtttaattt tgctgcgata gatgagcaaa ccaattaaga aagtaggcaa gataaataaa
     1861 caagcggcca ctagccccgt tgccaaaatc gcaagctcgc tactttctgg tatgaaaatt
     1921 gtgacaaacc ataccgctgc catcccgact agcaagccac ccaataaaat aattttctta
     1981 cctcgatcat aggatcgcca taatgcacct gaaacccggg gttcatccac ataatgatca
     2041 aagacttcgg ataagctctc gtcgtccggt tgagactcat atccaatatg aatggctaaa
     2101 gcctcatcgc catcttcaag ttttgccatc tctttgatga aggtaatttg tttttcggca
     2161 cgcttaggtt ttgcgccatc tttgatcatc atcgaatgga gcgcttcaaa agccttttca
     2221 cgcgccactt caggtgccat ctgttgctgt ttttcataaa attgccacat cactccaacc
     2281 agataaagct ggatcgtcat gaatttaaat tcaggcgcaa agtcatattt tttacgtttg
     2341 attcgagcca gctgctcatt cagaatcgtt cttgcgtatt ggatacaatt atttaaggaa
     2401 tcagtttcat cctcgctttt tgtttgattt atttgattat tatccatatt gcaaccttta
     2461 atttttctta gccaattatt taatgtgcac tattatatac ttgcaagcga tgtaaatcac
     2521 attactgtct cgttaagtgc tgatgagaag gttgatactt gatgcgaaat aaccaataac
     2581 gttctcttca gaagaaaatg gctcgttatg aatattaagg ttagtttaaa caaatcactc
     2641 gcataaaatc accttatcct cgatataatt tacgttttac ttaaatctct tttgctcatg
     2701 accgtcccca aacaaaataa ttttaaagtt catttcactg aattactcta tcaagcaact
     2761 gagaaaatat taccgcctaa ctgtgaaata tttctggagc tggcacgtcc taaacaagta
     2821 gaacatggtg actattccag caatattgcg ctgcaattag ctcgtttttt gcacaaaaat
     2881 cctaaagaaa tcgcccaatc gctcattcaa ctacttccag attcccctta tttggaaaaa
     2941 gcagaagttg cgggaaatgg ttttatcaat ttatttctca aaaaatctgc aaagcagcaa
     3001 tttttattac aggtattaca gcaaggcagt ttttttggga ctaccgatat tgggaaaggg
     3061 aatcaaattc aggtcgagtt tgtatcagca aatccaacag gccctttgca tgttggtcat
     3121 ggaagaggtg ctgcatatgg tgcaagcctt gcaaatatct tacgtgcaaa tggctattcg
     3181 gtaacccgtg agtattatgt aaatgatgcg ggaagacaga tggatattct tacattatca
     3241 acctggttac gctatctcga gctttgtggt gtatccatcc catttcctgg aaatgcttac
     3301 cagggagaat atgttatcga tatggccaag atgataaaaa aagcgcatgg cgatcgatat
     3361 gtacatgaac cgcatgcatt aatagcacgg gtcaccccgg caagtgagtc atcaatctcg
     3421 aacgaagatg aacaactcga tgaattgatt caggtagcaa aaaaaactct tagcgaagat
     3481 tatgcctata tacatcagtt tgttttgact gaacagttag gggactgccg gaatgattta
     3541 atggaatttg gtgttgaatt tgatacttgg ttttctgagc aatcactttt tgataatgga
     3601 tcgatcgcaa aagtaatcgc catgctcgag aaaaataata tgctctatca gcaagatagt
     3661 gcactttggt ttcgttcaac tcgatttggt gatgaaaaag atcgtgtcgt ccagcgtgaa
     3721 aatgggcaat atacctattt tgcttccgat atcgcttatc atctcaataa attcgagcgt
     3781 gggttttctc atatcatcaa tgtatggggg gccgatcacc atggttatat cccacgagtc
     3841 aaaggcgcaa tgcaggcact atccctcgat cccaagaagt tggaaattgc attagtacaa
     3901 tttgccgtgc tttatcggga tggcaagaaa gcctccatgt ctacccgctc cggtgaattc
     3961 gtcaccctaa gagaactacg caaggaagtc ggtaatgatg cagcacgatt cttttacgta
     4021 ttacgaaaaa gtgatcagca tttagatttt gatttggacc tcgcaaaatc acaatcgaat
     4081 gataatccgg tttactatgt acaatatgct catgctcgga taaatagcgt tattacgcaa
     4141 tggggtgaag cagtttcgat actttccaat gcgccaactg attcgttaag caatgaagcg
     4201 gaacttactt tgatacagaa attaattgat tatcctgata cacttgaagc tgctgcaaaa
     4261 gaacgggccc ctcacttcct tgctttctat cttaaagatc tggcgagtgc gtttcatagt
     4321 tattataatt caacgcgctt tcttgtaccg gaacaggaac ttaagttagc aaggctggca
     4381 ttaattgcag caatccaaca agttattgct aatgggttaa acttattagg tgtaacagct
     4441 cccaacgaaa tgtaactgca gttttataga acattatgag tagagattat aaatcaaggg
     4501 gaggagcatc atcaaaaggt ggtggttcgc ttattcttgg tattttcatt ggctatgcac
     4561 tgggtttagt cagcgcaatt ggtgtatggc tctatatcaa tcaagcgcct agtccttttt
     4621 taacggaaga taaaacagca aaccgaccga aagaagaact tttatccaaa ccaacccaga
     4681 cagaaaagag tgtcgttagc aacaccaata acaataactc gacagaagaa agtaagtctc
     4741 gctttgattt ttataagata cttcctggaa tcgatgaacc cgattcagga aatgtgccaa
     4801 atgctaaaga aatagcaaaa gcttctcccc aagctgagag cgttcagcgt ggggcaaata
     4861 gctatttctt acagatcggt tctttcaaga gtgcacatga agctgagaaa gtgaaagctg
     4921 aacttgcatt tttaggtatg atggctgcca tccaaccgac tgaatcatca gataaaggca
     4981 cctggtatcg cgttcgtgtg gggccattct cggatatcca tgaaattgat cagattcggg
     5041 cttccttaca ggaaaatcaa attgaatcaa atataattcg attaccaacg agtaatatgc
     5101 cttaatggat atgttatcct aaaatgattt ctaacattcc ttttaccatt tgcgatgcta
     5161 tgaaaaaact aaccaagctt tttatcttgc ttttgattgg ctgtgtaagc agtgtaggcg
     5221 tatcagtagc ttctactgaa attgttgaag gcaaagacta taccgttctt tcaacaccac
     5281 aagctaccga tgatgcccgc agtatcgaag tcatcgagtt tttctggtac ggctgcccac
     5341 attgctatga tttgcatcct catatcgatc aatggcgcaa aaatgcgcct aaagacgtaa
     5401 aattccgcta tgttccggca attttccgtg caaattggac cgttgctgct aagacattct
     5461 atgccatgga agcactcggt atttcgaaag atttgcatga taaagtttat gatgcgattc
     5521 atgccaaaaa aatcgatctc gctaaagaat cgactttgtt tgaatgggta gaaaaacaag
     5581 gcgttgatcg gaaggaattt gttaatgcct ataactcttt ctctgtgcaa aatatggtca
     5641 accgtgcatc tcaaatgacc aaacaatata aattaacagg tgtacctgca ctggtagtcg
     5701 aaggaaaata tctaacaagc ggtaaaatga ctgggttacc acgtgatacc attcttgttc
     5761 tcgatgcgtt aattgagaaa gcccgcaagg aaaaatcatc gcagtaagat taatcaagga
     5821 gtgccctgct atcacatttg agcgaccgtg aatgtgatga taatgaagta gcagtgatat
     5881 aatctgcagg tcactgctta cctttcccag gaattcacat gggcgttgcc aataaatcaa
     5941 tggcgaggaa atcgagcgat aatcctctgc ccccaaagtt agctaaatta ttacgtgagt
     6001 cgggttgtct ggttctaggc gcgatagcgc tatatcttgt actgatattg ttaagcttta
     6061 atcgagctga tccgggttgg tctcacagcg aaaatcttaa tcaaataacc aacacaggcg
     6121 gtaaagcagg tgcatggtta tccgatttat tgctatattt atttggtgcc tcagcttggt
     6181 ggttagtcgc tttatttatc gcagcgatta tctggagtta ccgacgaata gacatcgtca
     6241 gcttattcga tccacgctct ttaatcatat cgttgatcgg ttttgtggct ttgttgatgg
     6301 caagtagtgg cattgaggca ctacgattta ataccttgaa tattgcatta ccgctagcac
     6361 caggtggaat attaggtgaa acgattagcc aaggtttctc acgattactt ggagtaaccg
     6421 gcgcttcatt aagttttttc attttatttg caattggatt tagccttttc tcaggaattt
     6481 cctgggtacg gatcgctgag aaaataggtg aaaccattga gttatcctgc gttactttat
     6541 ggcaatcatg gttgacatgg ctagatcggc gggcagggaa attatcctct ttggaacgaa
     6601 gtgagcacgt tgatgatttg aaaaaagatt tatcaaccca tcctcccctc aaaatagaaa
     6661 tcccagatac atcgattccc aaatcgtctc gtatccttaa agaaaagcag acgccattat
     6721 tttcagagct cccagactca attcttcccc ccttatattt attggatgag ccgaaagttg
     6781 atgtagaact cttgtcgaac gagaccttgg aattcacttc ccgcttgatt gagcgaaggt
     6841 tacaagaatt tggggtagag gtaaaagtcg tatcggccca tccgggccct gtcattacgc
     6901 gttacgagat cgaaccagcg actggcgtaa aaggcaatca aattattaac ctcgttaaag
     6961 atttagcacg cgcactttca gtcgccagta ttcgtgttgt agaaactatt ccaggcaaaa
     7021 caaccatggg attagaaatc cccaacccta aacgacaaat cgtgcgttta catgaaattc
     7081 tcggttcaca agcttatgct gatacggaat cacctttgac tattgcactc ggtaaggata
     7141 tcagcggtcg gcctatcgtc tccgatttag gtaagatgcc gcacgcatta gtggctggaa
     7201 caaccggttc aggtaaatcc gttgcgatca atgcgattat tttaagtttg ctctataaag
     7261 catcaccaga acaagtaaga ttgattctgg tcgatcctaa aatgcttgag ctttccgtat
     7321 atgagggcat tccacatctg ctcacccctg tggtaactga tatgcgcgat gcggcgagtg
     7381 cattgaattg gtctgtagca gaaatggaac ggcgctacaa gctgatgtct gcgttaggtg
     7441 tacgtaacct cgccggctac aatcagaaaa ttcaagaggc taaaaaagcc ggtcagccgc
     7501 tggtaacacc gacccatatc gtatccgatg atgcgacacc actcgaagaa atgcccatga
     7561 tcgtggtcgt gatcgatgaa ttggctgatt tgatgatggt catgggtaaa aaagttgaga
     7621 aacttatcgc gcggctggcc caaaaagccc gtgcagcggg tatccattta ttacttgcta
     7681 cgcagcgacc ttccgttgac gtcattactg gtttgatcaa agcaaatatc ccaacccgaa
     7741 tcgcattcca agtttcgagt aaaattgatt cacgtaccat tctggaccaa atgggtgcgg
     7801 aagcgttact cggtcaaggt gatatgcttt atctcccacc cggaagcggt tacccacagc
     7861 gtattcatgg tgcatttgta gcagaccacg aagtccacaa agtagtggaa tacctcaagc
     7921 agcaaggtga accacgctat attgaggctg ttctcaaagc gggtgatgaa gaaggcgatt
     7981 tcaatgggga atccagtgag aataatcgac cttcgggaga aactgatttg ctgtatgatg
     8041 aagcagtggc aatcgttttg aaatcacgac gcgcatcgat ctcaatgatt cagcgacaac
     8101 ttcgtatcgg ttacaatcga gcagcacgct tagttgaaga tatggaacgc gctggtctcg
     8161 tctctgctat gcaaagtaat ggaaatcgtg aagtactcgc gcccgaccgc ggtgattaag
     8221 caatgcccgt tatggataat attattaaac tcgtcatcgc tattttgtag ggggttatca
     8281 ttcggtttgt aatcgtagtc gtcttattgt ttctttcttt agcggcacaa gcttctgctg
     8341 tagaacatct aagagaattt gtgcagaaaa cacaaacttt taaagcagtc tttcatcaga
     8401 aattactcga tcaaaatttt caagtcgttc aagaagcaag tggctcaatg atgtttcaac
     8461 gtcctggtaa atttcgctgg atttatgaga aaccctatca acaattaatc gttggtgatg
     8521 gtaagcgagt atggttttat gaccaggatc tcaaccaagt aacagtgcgc cagcttgatc
     8581 tcgcgattgg gaatagccct gctgcgctac ttgcgggtag cagcgcaatt gaaaacgatt
     8641 ttaatcttgc agaaatccaa attgaagatg aaattgattg gttggaagca acaccgaagc
     8701 ataaggaaac caactttgaa ttgattcgga tgggttttgc aaaaacaggt gaactgcgcg
     8761 aaatgattct tcgcgataat tttggtcaat ttaccctact ggtttttagt gaaataaatt
     8821 tcaaccctaa tcttgctagc gagctgtttc agtttgatcc acccagtggg gctgatgtaa
     8881 tcagtgactg agcttgattt ttcttctcga ccacccttat ccccaccgtt agcagaacgc
     8941 ttacggcctc gatcactcga agaagtaatc ggccaaactc acctcctcgg tatcggtagg
     9001 cccctacgta atgcctttga aacgggaaag ccccattcga tgattctgtg gggacctcct
     9061 ggtagcggaa aaacgacgct agctcgcctg atgtcccatt cgtttgacgc cgaatttatc
     9121 gcgatatctg ccgttttatc cggcgtcaaa gatattcgtg aagcaattga tcgtgcgaaa
     9181 agtatttacc agcagtcaca acgtcatact gtgttatttg tagacgaagt ccatcgcttt
     9241 aataaggcac aacaagatgc ctttttacca tatgtagaac aaggtttaat taccttcatc
     9301 ggggcaacaa ccgaaaatcc atcctttgaa gttaaccgcg ctttattatc tcgcgcccaa
     9361 gtttatacac tagccccttt atctcaggaa gaactacagc agctttttga tcgtacgcat
     9421 caatccgtat taaaaacact gaacttttca tatgaagcta agcagttgat tattcaattt
     9481 gcggacggcg acgccagacg attgctcaat ttactcgaac agcttagtat cgccgctgaa
     9541 gcagaagctt ataacacaat tgataccaat gaagtacgca aagcgttaac acgaagtcag
     9601 cgtaattttg ataaaggtaa agaagcattt tatgaccaaa tttccgcact ccataaatcc
     9661 attcgcggtt cttccccgga tgcggcattg tattggatgt gtcgcatgct cgatggaggc
     9721 gtcgacccca tgtatatcgc acgaagatta attcgagctg cagtagaaga tatagggcta
     9781 gctgatccac gcgccttaac catgacactc gatgcatgcc aagcttacga acgattagga
     9841 agtcctgaag gtgaacttgc attagcccaa gccacgcttt atttagcctg tgcccctaag
     9901 agcaatgctg cttacctagc ctataaaaaa gcccgatcca tgatcgagca tgatacttcc
     9961 cacccagtac cactgcatct gcgcaatgcg cctacacaat taatgcaaga aatgggctat
    10021 ggaaagcagt accattaccc tcacgacgaa cccgaagctt atgccgcagg cgaaaattat
    10081 ttcccggaaa cactttcccc tgttactttt tattacccaa ccacacgcgg actagaaagt
    10141 aaaattactg aaaaactcga gaaattagct gaaatggatc gacagcacaa aaagacatca
    10201 taggattatc aaaaaatcat caaagtggga atcatcagaa tcaaagaaga aacaagtgag
    10261 tttgattcct ttgatcttcg taatatctga gctcaaggac ttacgcagct tttcgcacag
    10321 gctcggtaga tagaatgatg agtgggcgtc ttgttatccg tacaccaact atgaatactt
    10381 cggttgaagt ccaagttttg gggcttgatc tctccgaaaa agaacagcaa agcggaaagt
    10441 atgtttgatt tgccgtgatt gctctagtca atgaagatca tcagatcctg gaacgcgatt
    10501 ttgagatgct tgatcgaacg ccaatttgaa atggcaacag attcaatctt catggtatcc
    10561 cccatttttt taagctagtt aaccgattta gctgtcatct taccaaactt gaataaattc
    10621 acaaatgtaa tgattggcta aaatctattg acaattagag ataattattc tatataaaaa
    10681 taggagttta taatttttga ttcctatatc atttataaaa cgcatttgcg ttgcaccgtg
    10741 tgccgattga ggttcggtga ggagttcttc aacgtggaag ggaaagaaaa acctgcttca
    10801 acaagctatt gatcattttc atagagttga taatttcgct ttactagtta aatttgaaat
    10861 catccgttaa gtaatcactg attgatctgc actacttgtt tgtgatctct agattttcta
    10921 ctggagaagc gacggttgtc gataaaggct atcacagcaa gcacatacga caaccgatag
    10981 taaaaaagtt aaatctgtga tgccagaaaa gtaacacgct ggcgaataac gcagataggg
    11041 attgggcttc gttcaatata gatagcggta tttgcgtggg cagcatcttt gcccagctaa
    11101 aacggtatcg agcaatcgta cacgatataa caagctgaaa agcaatacgg aagcttgttc
    11161 actaacacgc attatctcta tgtatgccca tgttaagtca acagacccaa gaaaatggga
    11221 caactccagt tcctaattta tttctggctc gttgctcaat actttgtgga tacttttcgc
    11281 aatggataca tctcagtcta tctcaatctg ggttttcggc attggtgaat acggagagga
    11341 aacctttcga agatttttgc acctcttaac ccaacagcac attgcgatac aagattattg
    11401 cactccttgt ccttattcag gaattatcta tagcaataaa gtcaccccag aactacttaa
    11461 caaattgtat gaattaagca accacggtca ggtgcaggtc cttgcttgtt gccaagaagc
    11521 aatttctgga gaagcaatct ggcagttgat ggaagcaggc gcctcagacg tgctggtttg
    11581 tacaaaagat aatctaatta ttcatgagat caccgctcgc ctccgccgtt gggaatccat
    11641 cagttctcta ctcaactctc caatagtcca aaatgcttta gttggtaaaa gccaaacgtt
    11701 gcaaaccata ctacggcaag tcatcgaggt cgcgcatttt accaatgtct cggtattagt
    11761 ccttggagaa agtggtaccg gcaaggaatt gctggctaat ctgattcaca cgctcgacaa
    11821 tcgttctgag aaaggcgagc ttatcattct agattgcacc acgatcgtgc cggaattatc
    11881 gggcagcgaa ttttttggac atgaacgggg agcctacaca ggcgccacct cttcacggga
    11941 tggcgtattc gcactcgcaa atggtggcac actttttcta gatgaagtag gggaattatc
    12001 cctaacaatg caaacacagc ttttgcgggt aatccaagaa caaacttaca aacgcgtcgg
    12061 tggcaatgcc tggcaacgta ctgcattccg gctgatttgc gcgaccaacc gcgatctcgc
    12121 aacacaagtc cagcgcggga aattccgtgc tgatctgtat taccggatcg caagctatat
    12181 ctgccatttg ccaccgttgc gcgaacgatc tgaagacatt ctaccgttgg cgaattactt
    12241 cctaaaaaaa aactgtccaa accaagatat tcccattatt gataagccgg ttgaagaata
    12301 tctcttacgt cggcaatatc caggtaatgt gcgggacctt aaacaagtca tcgcacgaat
    12361 gttatgccgc tatgctggta agggtccaat caccgtcggt tgtattccac ctgacgaacg
    12421 accgaatttg tcggaagagt ggaaagaatg gcgcgatatg aattttgagc ggtcaatccg
    12481 catagcgctt agtcaaggca tgggacttaa aacgatcagc aaaaatacgg aagaaacggc
    12541 aattcgtatt gcggtagatg aagcaaatgg caatttgcaa cgtgccgcac aacgtctcgg
    12601 tgtgactgat cgtgcgttac agttgcgccg ggcagcacaa cgtcaggaaa atagcgagcc
    12661 ttgatgatca cgctatcatc accggttgac tcgattgcaa aaaaacgaat tacgctgtgt
    12721 tgtactgttt ggatcaagca attgcctgat tcttttggtt agggaaaatt aagttttgta
    12781 aattgactcc cataatcaat ttttcttgct gtggcgaaag gcgtagtgcc ttaatctttg
    12841 ccaattcaat gccaggatgt agccatggcc catccgaacc aaacaaaatc ttgccggctc
    12901 cagctcgttt caccgcttgt tccagtaaat caaaacgccg cactccagcc gtatcggtat
    12961 agatattagg atgacgcacc aaatgatcaa tgatagccga ttgcgcacgc cagtcatcgg
    13021 caaaactgcc aagatgagga atgataaagt taacgtccgg atattcctgc gctaataact
    13081 cacaaaccga tacttctccc accacatcgt aaagcacagg caaagcgaaa gctcttgcca
    13141 cctcacagat ctcccgggta atgggtgcat catggcgatg cactttgatt cctacaaatc
    13201 catattgtct gacagcggtt tcaatcaatt catgtactcg cctgcggtct ctggccgcat
    13261 gcacaaatgc aaatccataa aacctgtccg gctggctggc tacaattttt gctacttctt
    13321 tattggcagc agcgtaatcc gaatgaaatg cggcaaataa aacactgcgc tgaattcctg
    13381 ccacggtggc acgccgcaga taatctctta atggtgcgcg cgtatcccaa gggccggtaa
    13441 acccatcacc gcttcccgca tggcagtggc aatcaataat catcattacc tccctaatca
    13501 gctattcttt tgattttcct actgcaccct actggtgcgc tgccatttgc caacttcagc
    13561 cagaaatcgg atactcgtct ttatgccgtt gaaaaaattc ggcaaataga atttctcatt
    13621 cgatccatga atacgatcgt ctggtagacc gaagcccatc agcaccacag ggatatgcag
    13681 aatatcctga attaaattga ccactggaat cgttccacca ttgcgcagaa atactggtgc
    13741 tgttccaaat ccgtgtcgat aagcatgcac cgcagccatt actacaggat gtttacgatc
    13801 catcagcaca ggtcgggatg gcgcagaagt gcgaatctgt aaccgtatcc ctggtggagt
    13861 aacttgcttg aggtaccccc ggaatagctg atcaatttcc cgtaaattct gatctggaac
    13921 caaccggaag ttcagttttg ccagcgcacg cgttggaatc gctgccttaa caccctctcc
    13981 ctggtaacca ccatgaatac tggttaccgt gatcgcaggt cggatcgtgg tgcgctcata
    14041 tattgaataa ccacgctctc cccaaccgac tcccactcca gcatcgcgga gaatgctttg
    14101 atcggatggc tctatttttt tcatgtaagc ccactcctgt gcatcccatt ttcgtacgcg
    14161 atcataaaaa ttgggaatgt tgatgcgtcc ggtgcgatca tgtaattgcg caatcatctc
    14221 acacagtact tgtaagggat tacgaatcgc accgcccaaa acgcctgagt gtaattcccg
    14281 attttgccca atgatctcca gctcaaaatt gattgatccc cttaaggcat aagtaatggc
    14341 aggttgccta gcagatggaa tctgggtatc cgacaccaca gcacaatcag cagcaagcat
    14401 ttgctgattg accaccacga aagcaggtaa atgggggcta cccatctcct cttcaccctc
    14461 aaacaagcac ttgatattaa cgggtaatcc ctgaccgctg cgaagcaata cttccaatgc
    14521 cttgacatgg gtaaagagct ggcctttgtc atccgaagcg cctcttccat aaagatgatc
    14581 tccacgaatc accggctcaa aaggcggaaa ttgccattca gcaagcggat cggctggttg
    14641 cacatcatag tggccataga tgagcaccgt aggttttccc gggatatggc acttctcagc
    14701 gtaaatcacg gggtgacctt gcgtggtcac gatcatcact ttttccatcc caatcttacg
    14761 cagatgattg gctaaccaag ctgcacaacg ccgagtatcc tgatcgtgtt caggttgtgc
    14821 gctcacgctc gggaaatgaa tgaatgcttt tagttcttct acaaaacggg cacgagatat
    14881 ttgctgataa tcaataactg gagaataaag atcaactggc gaagctagct gctttacagg
    14941 tttcacaaat ttctcgcttg attagataaa cctggtcccg atcgattctc aagccattct
    15001 aaacctaaca ggccacccga gggatctggt tattccggac aatcaagttg tccaactcgc
    15061 gaataagctg ggtatatttc ggaaattggc tgggttttgt tctgctcaga acgacaacaa
    15121 catagcgaat cctaccgcgc gcgattagag cacaatcgtg ccaataatcc gaccacacac
    15181 cgcatttgga tgcgaccaat cccaggcctt gtgggaaatg tttggtcata caacctcctc
    15241 gtaaccaggt ttgcatttct tttgacgata catcatccac aagcctgcct tgcgcgagca
    15301 acgtaaagta agtagccgct gacaacgcgg tcacattcgc gctctgtacc ccttttattg
    15361 gattattacc ccactgacct tttccatagg aagtggtaag ccataaacct ccgcgactgg
    15421 gatgaaaaag ccctgcctgc caagttaccg aagccatata cggaaaccca actcggataa
    15481 taagctctcc agcctctgca ttgtgcataa tgccttgaaa cactttgcgt gcttttacgc
    15541 taaaatccag ggtacccaaa ttgcctgtct tgttgcgaat atcaaacaac caaatcagat
    15601 ccggaaaacc actctttagc cctttagcct tccattgatt aactgctgcc aactccaagg
    15661 ctttgcctgc agcaatgcca ttgtttacag ccaagctgcg caaatcactg cgcagttgga
    15721 aagcagcgtg aagcgctaat atcttcggta cactggcacc atacatcgct accgtcgatc
    15781 cccatcccgc aaaatcaggt tggaccaatt tagtgcccga aagatcaacc aaaccgaccc
    15841 gaactcggtc gttccaggaa gcagattttt tttgcagact atcgtgactc aagtaatggt
    15901 ggaattgcga ggtcagcagt cggaccaagc aagtttgcaa gcccgtattg ctccgatctg
    15961 tgatgatgtt atctgcccca tcaatgaatc ctgggttcat attttttaca tttaacttgg
    16021 attttacctc agtgataaat cgcccaggat ggactgggtc aacttgcttg ggaacatggg
    16081 tatgatcaag gcagatcaac cctttgggac aagcggtcac tccactcgaa gaaggcaatc
    16141 gctgactttg tgagacctca gtttcccagg aaaaaccacg ccgagtatta ataaatctat
    16201 catattcacg gtcaccgaat ttaaagttaa atgcaaatga ctcaattgga aagtgaattg
    16261 gggttttcat ctacactccc tcctttcttg tttaggcagg atgcctttgc aaagtatttc
    16321 atgtgtccca tcacagcgat gtgacgatca cggtaaaacc aatgaacgct accggtatta
    16381 tctaccggta aaaccaccta atcctggact accacttcct agtcgatgag gaagattcgg
    16441 aacatgcatt ctagctgctg ccaccaaagc agtattggcg atcgcttcgg gactggcatg
    16501 cggattagcc aatgctgctt gtctagccgc tgtcgcggca atgcgaacaa aacgccgagc
    16561 catttccagt tccacttctt ctgaaggcaa accgttaaat tcaagctcta gtgctttact
    16621 gacagctgtt ccaattgcgc caccgatagc cgtaccaaca cctggtatgg ggataaacga
    16681 tcctaacgct ttgccaacaa tcggtagcgc aaccttaccg atcgctttca aacctttacc
    16741 caatgcgggc aacactttct tgccgatgaa acggccaaat ttcttaagtc ctctaccgat
    16801 tcccttgaac aatttaccaa gaaataaatc cagctcttct tcgttagaaa tagacaataa
    16861 ttcagcagcc agctctagct cttccgcttc gctgaagggg tattcgagtt ctccatataa
    16921 atcccgatgg ggagaaaatt cccaaaatgg gttggtagca ggatctagaa gcaacgatga
    16981 ctggctcgcc ggaattgaag gataggtagt aatcggttgg ttatagacat cggttcccgt
    17041 tgccgtataa gccttattta atagatggat cagaggcttt ggaagaatat tttctacggt
    17101 cagaggccct aatacggtag caatggtata tttctcccgg tgccattgtg gacgtgttgc
    17161 tgctgcccga tgagtcctaa acaataatcg cgaaatggcg cgctcaacct gtgtatcaag
    17221 accacccacc ccacagctgc cacctctgca tccactaaca cgagccagat agaacctcag
    17281 atccggcagt acatgctgaa ttttaccttt aatcttgcgt ttgactgttc tagatctgta
    17341 tttcggtaaa cgggtattaa agtccgaagc ctgcccaact ttgagtattt taggcatttt
    17401 cccgctaaca tccactgcaa tatatattcc accgcctatt tcagtaattg cttcatcaag
    17461 tgtatagccg cgaggattag cattattagg taaccaagtt aattgggact gcaaatgagc
    17521 accaggcagc ccaagtgttt gattctctaa ttcttctcca ttggcactta atgctgcagt
    17581 gttactggtg cagattgatt tgatggcctc ttccgtatcc ggcccaacga taccgcttgc
    17641 tcttattcct tgctgacgct gaaaattccg aatgacactg cgagttttag accccatgat
    17701 tccgttcacc ggaagttgcg tccccatcgc gtgattcaga caatcttgta cccaacgagt
    17761 acgttcagaa cggggtggtt ctggttcact ggaatagggt tcgtctacaa tctgaatagg
    17821 ctcatagcgc gcacttccaa atggtcgccg aaatcccctc aaagcagatc gcgatcgctt
    17881 aatgggcgga cgtttggtgc ggcttgacct caacccggaa cgaataccag actgtaaatt
    17941 acgactttta gggcctgcaa aacggcggat tcctcgctcg ctttctactt ccaatatttc
    18001 tcctcccatt tcaggagtaa gttctaaagg tatggtttca aaattcattt cttgattcat
    18061 gattcacctc tcgcatagag ttatcttttt agaacccaga atacgctggc ttaccactgc
    18121 gatttgcaac tctgctgata atgcgtgtca atgtattgag taggtcgata cattgatcaa
    18181 tttcctcgct agtgtgccgc gctgtaatta aaaaacttaa gtgcgcctcg ccgctattgc
    18241 gaggccgtga caataccgct tgaacaccct tctgggatag gtagttatgc acgcgcaccg
    18301 cattatctcc aacgattcct cgcaaaattt gtacaggaaa ccaaccaccg atggcacccc
    18361 aaccgatggt ttccaaacct ttgcgaaatt gagcaactcg ttgcgccaat ctatgccgta
    18421 acaaatcccc gtgagcttgg ttcacattaa gtgaatgcct ggcagcctga atgactgcta
    18481 ctgacggcgg gctaccatgt atccgggtat cacttgcgga tttaaactgt ttgactagcg
    18541 ctttacttcc cgcaagaact gctaccggca cacccaaacc ttttgccagt gatgaaccca
    18601 ccacgatatc cggcccaaaa tttccgctcc agcgcagcag gccacccccg ccttctccat
    18661 aaggaggaca ccatgctggt gacgctccca aaattcccaa agcttgcgtg tcatccaaca
    18721 ccagtaaacc accaaaacgc cttacgatct ccagataatc attaatcggc gctgcatgcc
    18781 cacacgatgg acagaaacca tcggcaatga ctacggggtg cttcttcctt tgtgcatgca
    18841 acttgatttg cgccaacaat gcttcaggat cgtgatgtcg gaaaatataa acatcacccc
    18901 ctttggcacg aaactgctct accccccagc gagcaatcgc ataagcgcca tcatccagaa
    18961 ataatgccac tggctgctgg tgcaacatac caaacaagtc ccaaaataga tgcaatgtgg
    19021 aaggtagcaa taccccttgt tcgctccctt gtaactgtgc caatttccca gccacttcct
    19081 cagcaccagg tggctcggca agtgctgcag gaacgcccag tgttagctgc tgccaggatt
    19141 gcaaaagttg actggggtga tgcattccca ggtatagtgc tgaagcaaag ttgatcaccg
    19201 ccatgcgcct cacatcgccc gttgctgact tgctaaccgt ttttgcagta gtactgacgg
    19261 caacgttgaa tctacacgcg tattgaccat tgggtctgct gacaaatcga cacctgttgc
    19321 tgcacgatag gcatgaatat aaccctgtgc ttgtggtcgg aaaaagcgcg cccaattgag
    19381 cgcttttaca ggctcaaatt cgtcgctcca atgtccccaa cgaatggaaa gcaaaagctg
    19441 ctcgccaaac atacccagat tacggaaatg cagcacgctg gtatcggtcc agccttgcaa
    19501 tttcttcatg gcatcgacac gatccatcca gggttcaggg taggccacca taatgcgtgt
    19561 tggcaataac tcccggaact cgggtctggc caacaaccat tgctgcataa gcatttcaat
    19621 gcgagccgtc gacggcaagt cgccaaactg attgtgcgcc ccctgagaaa gaatcaaatg
    19681 tacctctttc agggcattga gtaccggaaa cgcatcggcc ttaaccgtag tatcgtcatc
    19741 ctgtttataa aatacggtgc acaaccgcaa cagatggtga aaagcttcaa gaaatttcga
    19801 tcgcgtatca gcaggtcgga aattctgtac ggctttacct tccagacgca agccataatg
    19861 atgatcgtac tcataattgc gccgattaac cgtcaagcga tgctgttcat cctggatata
    19921 accccacagc acattgttga gtggccgtag cggatccatc tcgagattgg aaagtggatc
    19981 ttgttcggag ccacgtacat tctggaatcg ccttgttatg gcattggtgg tttgcaccag
    20041 catcccttct tcgtgccaat aggaccatat gagttcaagc aagcaaggat tggtaagttt
    20101 ttcttgcagc agcccaaaac attcgtcggc atcaatcttt cctgttccgt tgtttgccgc
    20161 atcttcaaat ggtttgatct taatcgggat atcgggtagc ttacggcgga taacagccag
    20221 atagggtaat acatcgccat taccatcaat tcccactaga taatcatttt taaaagcctg
    20281 attcaaacca tagggtggta tgggtagatc acgccgattt aaataagctt cgtctttaat
    20341 agcatcaaag ggttgtagat catcacgatg cacaccacaa ttcaccatca caaacgcctc
    20401 agtggctgct ttgacgacac gatacgcgtc ggagtctgta aacggtaaga aacgattctt
    20461 caataattta ccccgccata tcctattttt ttcatcatag cgatcgcttt caaattggct
    20521 tagctctcgt ggtttctgtt tgccacagaa aatccagtcc atgaagatca gataattatt
    20581 aaatgaaagt gcttctgcgc tattacgaat acccgtccaa aatgccgtat cttctgtgaa
    20641 agaagtttct gttcgggtaa gactggtcgt cgttctagca tcgatatgct gtacattccg
    20701 catcgctgtg cctgctaagt ttgtagcaat tgatgagccg tcgggatgct tgaaagctgc
    20761 attaatcgaa taattgccct gcataccggt ggtatcgata gctatctcat aaactcgatc
    20821 cattccatcc tcaaaaattg aaggctgaga caggatgccc tgattagaag acaatttaat
    20881 ttctggatta cctaaagctg cactatgcga aatacgcaat ttagctggtt gacctgccac
    20941 aatatcctga caaagttgct cttcagatct tggaccacac atcgccagtt gctgcgtaaa
    21001 atttaccggg gctccaagac taatccgttt cgtacaatca caatcaatcg aaatcaattc
    21061 ttcttgtagc tcatcgtctg aacatccgac cggctctcca ctaaaaccga aagcattctt
    21121 aaaagctaag ccataacgat gcgcatcacc tcctagaagc ttaaaggttg catagccatt
    21181 ttcatcggta gttcgagttt caatagatcg ccattcaccg agattccatt ccagaacttc
    21241 aatatccgct cctctgatcg gtagtttatt ctcacccgag tcatgcaagt gaactcttag
    21301 cgtgccatgg catggggcct cacagttctg tatttttttg gaccttatat tcatatcatc
    21361 ctcctgatta gatcgttaaa atgatttcgt tggtattggt tggtattggc ctgttatcga
    21421 cgataaaggt tatactgacc gcattgcaca ggtcgcagat actgccaagg tgctgcgcag
    21481 cgcccagtga gtcagcagtt ttcccagcag ggcgctttcc tgttctggcg tcatacgccc
    21541 gtcggcacgt gcttgaccga tcacagcaaa agctagaacc ggtgatactt ggtacatttt
    21601 tgctggctgc tgttgccatg ctagaaacag tgccttaagg cgatgaggtt gtcggttttg
    21661 cacctgtaag acctcagcta ggctacgccc atgaaggctt gcagggcggt gattgactag
    21721 aagcgccaca aaacccggca atgttgcttg taatgcctga taattcagct gttgtgctgt
    21781 atccagccca tctattggat agtaggcttc ccatagctgc gctaggcgtt gccattgtgg
    21841 atgcggataa agtgcttgcc cgattgcgca acttagtttg acgcgtatcc acggggatgg
    21901 atgcggatca tcgcggttca agcggaatac aaatgcgcgc ggcaaactta cgacacccat
    21961 cagcccgagc gttgaagcaa tgccaatgcg agcaatcgac caaaaatcag cgataatttc
    22021 tgaaacccac cgctcccaca atgaccaagc cttactatat gcgccactac cgcgttgcat
    22081 tccttgcaaa accggccgca aagaattgac caagttaagc aatgctgcag attgatgccc
    22141 tacttcatga atcaaggaag aagcaatacc gctccccacc atgcgttccc gcggaatccg
    22201 aatcactgct actggatttt ctccaccacc tggtaatcgt gttctggtgc ggcgaattgc
    22261 tgcaccaaca ccccgatcca gatagcagat aaccggcggc acctcgtaat aatgtccagg
    22321 aatcgccagt gcatccgctg aaacgatttc aagaccagaa agccaaacac caatctcgct
    22381 ctcactacgc tgcgtcagcg catcgttgaa taaatcaaat tgcgtcagca ccgcattgaa
    22441 tcgcaaccgc aacaacgaaa atagacgctg tgcgtgggca ggttttgccc tacggccaaa
    22501 tggcccgcgt aaccaagcaa tgtagcgatg aattagcctt ctcaattctc gccgtccctt
    22561 catcagatac tgctcaatgc cgacttgcgc agcgggtaac aatgatgctg cgggtaacat
    22621 ggtttcatgc aaagcaaaag gcttgacacg tttcaagcga gtcagtaatg cacgggcctc
    22681 ttgctcgagc atccatgtag cgtaagaagc ggcagccatg gtaaattaaa ctccaagcaa
    22741 aataattttg cttccacgcc gtacccaacg gccactcatc gtaccccgac cgcaagcgga
    22801 acagggccca ctagttacaa ctggattatt gcgaaccaga cctggggcaa gctggcgcgc
    22861 agccgctgct acggcagact gtgcaaccgc tttcggattg actgcctgcg gtgataaggc
    22921 tacgtttttt gcagcagtac cggcaaactg aacgaaacgc ttagccgctt catactcctg
    22981 atcctcatga ctcagccctt ccaattccag gccaaaaatc ctccctgcgg ttgaagccac
    23041 ttggctgcca atcttggcac cactaccgcc accgaaatat ccaccaatcg cgcttcccac
    23101 catcgggagt gcttgcttgg ctgccccttt caataaacca ccaagctgct gtcccactgg
    23161 agacttaata aattttccag caac
//
LOCUS       DAOQLJ010000017        22816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_773_length_22816_cov_6.349238, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000017 DAOQLJ010000000
VERSION     DAOQLJ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 22816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22816
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_773_length_22816_cov_6.349238"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..2192)
                     /gene="fusA"
                     /locus_tag="PK522_02445"
     CDS             complement(99..2192)
                     /gene="fusA"
                     /locus_tag="PK522_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNC40626.1"
                     /translation="MSRKTPIERYRNIGIMAHIDAGKTTTTERVLFYTGVSHKLGEVH
                     DGAATMDWMEQEQERGITITSAATTCFWSGMAKNYPEHRINIIDTPGHVDFTIEVERS
                     LRVLDGACTVFCAVGGVQPQTETVWRQANKYKVPRLAFVNKMDRSGANFNRVQEQIKS
                     RLKALPVPIQLPIGAEDKFEGIVDLIKLKAIYWDETSQGMRYDERDVPEDMLDSVKAA
                     RESMIESAAEASEELMNKYLESGELAAEDIKNGIRIRTLANEIVPMLCGSAFKNKGVQ
                     AMLDAVIDFLPSPLDKPAIQGLKSNGEKDERHASDEEPFSSLAFKIATDPYVGQLIFF
                     RVYSGVVNSGDTVYNSVKQKKERVGRLLQMHANQRDEIKEVRAGDIAAAVGLKEVITG
                     DTLCDTEHIITLERMDFPEPVIHVAVEPKTKNDQEKMGIALNRLAQEDPSFRVRTDEE
                     SGQTIISGMGELHLEIIVDRMKREFSVEANVGAPQVAYREAIKKKVEIEGKFIKQSGG
                     RGQYGHVWLRMEPNEAGKGFQFIDEIKGGVVPREYIPAVEKGLIDSLTNGVLAGYPVV
                     DVKIALFDGSYHDVDSNENAFKMAASIAFKDGMKKATPILLEPIMSVEVETPPDFMGN
                     VVGDLSSRRGMIQGMDDTVGFKIIKAEVPLAEMFGYSTVLRSATQGRATYTMEFKHYS
                     EAPKSVAEAIINKNK"
     gene            complement(2258..2728)
                     /gene="rpsG"
                     /locus_tag="PK522_02450"
     CDS             complement(2258..2728)
                     /gene="rpsG"
                     /locus_tag="PK522_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNC40627.1"
                     /translation="MSRRREIPKREILPDPKYSNIEVAKFVNVLMTRGKKSVAERIIY
                     GALSQVEKKTGKNPVEVFTIALGNIRPIVEVKSRRVGGANYQVPVEVRSVRRNALAMR
                     WLRDAARKRNEKSMDLRLAVELIEASEGRGGAIKKREEVHRMAESNKAFSHYRF"
     gene            complement(2774..3151)
                     /gene="rpsL"
                     /locus_tag="PK522_02455"
     CDS             complement(2774..3151)
                     /gene="rpsL"
                     /locus_tag="PK522_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNC40628.1"
                     /translation="MPTTSQLIRKPRQDKRVKSKVPALESCPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGFEVSSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQGRSKYGSKKPKVS"
     gene            complement(3335..5362)
                     /locus_tag="PK522_02460"
     CDS             complement(3335..5362)
                     /locus_tag="PK522_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HNC40629.1"
                     /translation="MSKPVSLLFGVHAHQPVGNFPEVLEDAHIRCYKPFLHVVHRYPD
                     FKFAVHFSGWLLDYLFDHHPEDMKLLKAMVKRGQVELFSAGDTEPVLATIPNRDRIGQ
                     LETFSKKLNKKLGQRPQGAWLTERVWESTVIPALVDCGISYVIVDDYHFLCAGKTKAE
                     LNGYFTTEEDNQRLDLFPISEALRYKLPFSPASDAIAYIESLSEHQAASQRVAAIYFD
                     DIEKFGIWPETYQWVYEKGWLEQFIQGVLASPQIMTSHYRDYHSSEKSRGIIYLPTTS
                     YIEMNEWTLPADLANRYADLVQQSKVSGSYEHNKPFLRGGIWKNFFSRYPESNWMHKR
                     MLSLSQRFASLPVKQQSATMREKLYASQANDAYWHGLFGGLYLPHLRRAVFNNIVELE
                     GMLDLIAPRPTIYIEDTDLDGVEEIYLNNGILQVISKLDGAASICEFDAYKLKHNFGD
                     TLRRQEEHYYHKIKYDMPNLQQSGSGIASAHDRLSFKHQISSADLEGGRAAQALFVES
                     IDDHLVMYQFESKHDHGIKFKADFDGYKINKKIELIENQLLITYTSKSRVEGYLKTEF
                     NLAMPSCDGVGGKYIRDDLVLGGFGQELELMDFISIFLEDSTLKGRLLLSVSNESRFS
                     SKPFYSVSQSEGGFEKIMQAIKLVLVWPCIPERLTISLTVVELGGVVEVYS"
     gene            complement(5470..7176)
                     /locus_tag="PK522_02465"
     CDS             complement(5470..7176)
                     /locus_tag="PK522_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNC40630.1"
                     /translation="MNSLNLILMWHMHQPDYRDHETGEFLLPWVYLHAIKDYTDMAYH
                     LEKHPRVKAVINFVPILLEQLEDYSDQFLQETLRDPLLRLLITPDLNLISLEDRMFIL
                     NQCFQSNHDTMLKPYRAYQHLRDIYETLIGNGDAEMSYVSGQYLADLLTWYHLAWTGE
                     SVRKNCELVGKLMQQGKSFTYSDRKQLFELIGTLIQDLISRYRKLAVSGQIELSTTPH
                     YHPLSPLLIDFNSAYDSLPDAVLPSSSDYPGGRDRVAYHLELAKESHKQRFHSYPFGV
                     WPAEGGISKDFVEILAASELKWTASGEGVLAQSLRKSYPDRQLPERGEYLYRAYKVLT
                     NKEDVICFFRDDKLSDMIGFEYAKWYGRDAAENFVHSLERIHHEVKDAEDPVVTIILD
                     GENAWESYPYNGYYFLNDLYEMIGNHLAIKTSTFRDYLVHLKESDTEAVFLPNLAAGS
                     WVYGNFSTWIGSHDKNLAWDILCKAKKSYDAVLKIGNLTDEEQAAAKRQLASCESSDW
                     FWWFGDYNSSQAVASFDRLFRKNLANLYSLLKQPIPSELSEPISYGNSNARMIETMRR
                     SN"
     gene            complement(7215..8462)
                     /gene="glgC"
                     /locus_tag="PK522_02470"
     CDS             complement(7215..8462)
                     /gene="glgC"
                     /locus_tag="PK522_02470"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNC40631.1"
                     /translation="MATRNTLALILAGGRGSRLHNMTDWRAKPAVPFGGKFRIIDFPL
                     SNCVNSGVRRIGVVTQYKAQSLIRHIQRGWSFLDGRFKEFVELLPAQQRTEETWYQGT
                     ADAVFQNLDILRNHDPSYVLILGGDHIYKMDYSKLLDEHIQKGADMSIACLEVSFEEA
                     RAFGVMAVDEQWRITSFAEKPENPTAIPGQPDRALASMGIYVFNSQFLFEQLIRDHDD
                     NASTHDFGKDIIPYLVNSQYRVYAHRFTNSCVNMASGIPYWRDVGTIDAYWEANIDLT
                     TVTPDLNLYDEDWPIWTYQEQLPPAKFVFDDDNRRGQALDSMVSGGCIISGATIRRSL
                     LFSNVQVRSFGIIEDSVILPNVDIGRHARLKNVVVEKNCIIPEGLRVGFDAEEDGKRF
                     HVTKKGITLITPEMLGQGIHELR"
     gene            8666..10873
                     /gene="glgB"
                     /locus_tag="PK522_02475"
     CDS             8666..10873
                     /gene="glgB"
                     /locus_tag="PK522_02475"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNC40632.1"
                     /translation="MKSKQLSTVSYPLQHELLTARLHDPFAYLGAHPVSNETVVRAFL
                     PHAEKVWLQTEAGFTEMACAHVDGLFEWRGKDSIPKCYRIRMRKKNDAAPENLHEFYD
                     PYSFPLQISDLDLYLFNEGKLHQAYRMLGAHHLTINDIAGIRFSVWAPNAERVSVVGD
                     HNGWDGRVHPMAVHHQSGVWELFIPGFPGHGIYKYEIRNRHSGEILLKTDPFANYHEI
                     RPNNAAYTPTHSSFTWSDDLWMNQRKHWDWLHAPLNLYEIHVGSWKRHPDGKFFNYRE
                     LAHHLIPYLLEMGYTHVELLPISEHPLDESWGYQTTGYFAVTSRYGSPEDFKFFINAC
                     HQANIGVILDWVPAHFPQDSFSLARFDGTALYEHEDPRLGFHHDWGTFIFNYGRNEVK
                     SFLLSSAHYWLNNFHLDGLRVDAVASMLYLDYSRQHGEWLPNKYGGRENLEAIDFLRE
                     LNIMVHDAFPGALTFAEESTAWPAVSRPTYVGGLGFSMKWNMGWMNDTLSYIRQDPIY
                     RRYHHNQLTFNQLYAYSENFVLPFSHDEVVHGKGSLLGKMPGDAWQKFANLRLLLTYQ
                     MTCPGKKLNFMGNEFAQAREWQVNQQLDWNLLDYKLHRGVQLAIHDLNHLYLETPALH
                     QLDFEQAGFCWADCNDADQSVISYLRYAQDGSFSLIVLNFTPVPRENYRIGVPARGIY
                     HEVFNSDSTYYGGGNVGNPKQIFSVAEPWLEYQDSVIITLPPLAGIILALTPA"
     gene            10979..12079
                     /gene="nadA"
                     /locus_tag="PK522_02480"
     CDS             10979..12079
                     /gene="nadA"
                     /locus_tag="PK522_02480"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNC40633.1"
                     /translation="MQANTIDFENYELLQDEICAQRIVAAKAKLGKKAVILAHHYQRA
                     DVYCHADLTGDSLKLSYLAAQTDADYLVFCGVHFMAEVADILSSPQQIAILPDLSAGC
                     SMADMANLSKVERAWRELSEILNPDEKITPVTYINSSADLKAFCAEHGGIVCTSSNAT
                     KVLQWSFEQREKVLFFPDQHLGRWSGYKMNIPLDEMLIWDFDEPMGGLSEAQIKKAKI
                     LLWKGHCSVHQMFQPQHIIRFRNQYPDGIVISHPECSFEVCKASDYVGSTEYIIKTIR
                     EAAPGTRWLVGTELNLVGRIAEEFKAQGKIVQFMSPMVCMCSTMARIDPQHLAWSLEN
                     LVAGKIVNQIKVPDQEAQLAKLALERMLAISK"
     gene            12150..12902
                     /locus_tag="PK522_02485"
     CDS             12150..12902
                     /locus_tag="PK522_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNC40634.1"
                     /translation="MTNTLHIATYNIHKGFSLFNQRIVLHEMREKLRTLGTDIIFLQE
                     VVGAHRKHAMRFSNWPSNPQYEFLADSVWRDFAYGKNAVYDEGHHGNAILSRFPIIRW
                     ENIDISAHRFEQRGLLHCEMDIPGWQEPLHCICVHLGLFKRGQAMQLRAIEKRLTEII
                     PPTAPLVIAGDFNDWHGSAKRILTESMKLVEVFEQTHGRTARSFPSLFPLLRLDRIYV
                     RGFQVKNAVVHQSPTWARISDHAALTANIVRR"
     gene            12899..14074
                     /gene="clsB"
                     /locus_tag="PK522_02490"
     CDS             12899..14074
                     /gene="clsB"
                     /locus_tag="PK522_02490"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HNC40635.1"
                     /translation="MNNPDFIDHNKIILLNNGSQYFPALVSAIEQAKYEIHLQTYIFK
                     YDATGIIISEALTRAAKRGVAVHLLMDGFGSLDFPNKIIEKLITEGVQVLIYRKELLT
                     FKFLKHRLRRMHRKVVVIDAQIAFIGGINIIDDHDPRASQPLPRFDHAVRIEGPLLAN
                     IYPVVKRLWMLVAWVHFKKRWIKKLPELKPQLKSSGNQRAALVIRDNIRHRRDIEHAY
                     LKAIATAEEEIIIANAYFLPGRNFRKALIHAARRGVRVILLLQGKIEYRIQHYASRAL
                     YGNLLEAGIKIYEYHKSYLHTKVAVIDKYWSTVGSSNIDPFSLLLAREANIIVTDHAF
                     SSELRANLQQALHESNPVSARYWKKRAWLDRLLNWISYYTLRFLQGTLGYGQKSTRI"
     gene            complement(14085..14849)
                     /locus_tag="PK522_02495"
     CDS             complement(14085..14849)
                     /locus_tag="PK522_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HNC40636.1"
                     /translation="MVKPVLLVALVILLLTHYLTEHVNASWRTASRESVGLAPSPEQT
                     PEALIQVYGARAYGWRGYFGVHTWIAVKPSNANAYTVYEVIGWYLRRGGSVVAIHEQI
                     PDRRWFGNMPQLLIEKRGEGVDALIERVEQAAYQYPYHYEYWIWPGPNSNTFTAWISR
                     AVPELDLDLPPTAIGKDFLGKEWVATAPSGHGWQWSMFGLLGVTVSRVEGFELNILGL
                     TFGIDPDPFAIKLPLIGRIDIPILDLLSASSTIQEK"
     gene            complement(14914..15798)
                     /locus_tag="PK522_02500"
     CDS             complement(14914..15798)
                     /locus_tag="PK522_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNC40637.1"
                     /translation="MRKLILLTILSLVVTAVSANQSSSELFLDDLPVIDKDPNELFAL
                     AVKYEHAEGFPEDKTKAAELYCEAARLGSADAQFALGWMYANGRGVERDDSIAAQLFE
                     MAAEQGHHYAQKMIQYTPAMLPVSLPACLIPSIEIFDDEEDSDYTYPEGPVLHLVHKL
                     APDYQLDPKLVLALISIESAFKVKAISPKNAQGLMQLIPATAQRFQVKDTFDPEENIK
                     GGMAYLRWLLAFFKGDVTLAVAAYNAGEGAVEKYRGVPPYRETLNYVEKIRGRYKKVT
                     HPYQAEVVSRASILVSAR"
     gene            complement(16196..16522)
                     /locus_tag="PK522_02505"
     CDS             complement(16196..16522)
                     /locus_tag="PK522_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HNC40638.1"
                     /translation="MMLLPGQRMKEEVKQRLQRTETWVQGFFILFFVFVSGVVESLII
                     MIMFFQYVSILVTGKTNTHLLRLGKYLAAYVYQIVLYLTFNTGQRPFPFTIWPTEASD
                     KEGSKT"
     gene            complement(16652..17356)
                     /locus_tag="PK522_02510"
     CDS             complement(16652..17356)
                     /locus_tag="PK522_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNC40639.1"
                     /translation="MLISNLDSLSIRLSLLLNDCLRLCTSILSGGLLLLPFLATEVLA
                     QQSARNVEGCSIRRGTFCVDMSLNGADLQNTNLANSQFTRSILSGANLTKAVLDEANL
                     SDAQLRGTILNQASLIKTNARRARFNDAQLVGANLRSAILQNADFSGANLTDTVLTSA
                     DLTNAKLVGASLNNANIQRANLRSAHLQNASLVNANLQGVDFTGADLSNTDLSGANLD
                     QAIFTMARTKGCKGCP"
     gene            17617..18186
                     /locus_tag="PK522_02515"
     CDS             17617..18186
                     /locus_tag="PK522_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40640.1"
                     /translation="MRTSLKPIKGILIYTLILFTVSLIYFIYAFSVYPSSEEQETYLH
                     EIGEGFGKTGLALLGLIYFRTFLKLLLGKGKLAQRLLPEYQPPFDANLFDQLLGFLNR
                     THVYVGIAAVAILLLHAAMMGLTQHLHILFFPALLALIIWQALFGLFLTWRYSTTELK
                     QFSYLVHAQFVTGIAIGIFAFFGHILIDD"
     gene            18358..18534
                     /locus_tag="PK522_02520"
     CDS             18358..18534
                     /locus_tag="PK522_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40641.1"
                     /translation="MNKNKRVASLLLLITLIPFLSGCWTLVAAGAGAYGGYKMKEKGY
                     TLQNPITKEKNKKD"
     gene            18565..19716
                     /locus_tag="PK522_02525"
     CDS             18565..19716
                     /locus_tag="PK522_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNC40642.1"
                     /translation="MFSLSLRFSLITSLMAILLLSGCQSLFMSSAPLPAPVAKPLPVA
                     IASHEFSFDPTQEDVVGTLQITTVNEEDTLSDIARRFNLGYEEIVSANPGVDPWIPKV
                     GTPIVLPTQFILPEGPRRGIVINLAAMRLFYFPPKSKNSKLQKVITHPLGIGRVEWKT
                     PQGMTSIIAKKENPAWIPTASIRKEHAANGNPLPAIVPPGPDNPMGTHVLRLGWTNYA
                     IHGTDKPPSIGLRGSHGCLRMYPEDITGIYDAVPVGTPVRVVNQPHLLGWRGDHLYLQ
                     TYPILEDDKRNHKQRLRQSLANIRSTSKAKLGVRPQNKIDPVLLKEIIQSERTVSLPI
                     TQAPTPLLTLLDQHALKVQNTLPRYATWSGDSSQQLTVAEIVKMAEGEP"
     gene            complement(19738..20259)
                     /locus_tag="PK522_02530"
     CDS             complement(19738..20259)
                     /locus_tag="PK522_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HNC40643.1"
                     /translation="MRKVMLLIGCSVAALPSVFAESIIRPGLWEVTTKSDLLQLVPHV
                     PAEQMQQITRLAKQYGLEMPQISNGAANSKMCITPEMAKQEVPSYFFEGQTGCTVKNA
                     SRTGNRYQMELTCSNAQFKGEGYAEGVFMTPESFSGQAEFDSVIQGSPIVAHAETSGR
                     WVGEHCDVVTPIR"
     gene            20484..21824
                     /gene="msrA"
                     /locus_tag="PK522_02535"
     CDS             20484..21824
                     /gene="msrA"
                     /locus_tag="PK522_02535"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNC40644.1"
                     /translation="MKTILTAAILFIMIITSLAIGKTITQNSGKSNQIMNSTTDYIVF
                     GMGCFWGAEKRMSEIPGVIDVESGYAGGDIVNANYQQILAHEKALRAGKAEARNHAEV
                     IKVTFDPEKVSLETVLAKFWENHNPTQGDRQGNDIGSNYRSAIYYHNPAQLAQAQVTQ
                     QVYQHALTAAGYGAITTEILPLKNYLTAEEYHQNYLQKNPNGYCGLGGVGVKYPQANQ
                     TLSQNPNATDAKIAELLKPLKGSELNHAQQLVIFEAENCEYCKLFDQDILNYWQAEVP
                     VVATLNSNPPEGWTLEKPLFAAPTIVLFRNGQEVARYTGYNGEITNFWKWLGFQLLSP
                     EQQKIAFQHGTEPPFTAPNLDEKRPGRFVDPITGATLFLSKTKFDSKTGWPSFFDPVE
                     GSVTLHNDDSLGMRRIEVRSASSGIHLGHVFNDGPAPSYKRYCINGNVLKFIPD"
     gene            complement(21860..>22816)
                     /locus_tag="PK522_02540"
     CDS             complement(21860..>22816)
                     /locus_tag="PK522_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNC40645.1"
                     /translation="DKNLKYHGLVQAFSRTNRILNEKKSQGNIVCFRNLKAATDEAIA
                     LFSSKNAKETVLLEPYESYVQQFNQATEALLAIAPTVASVDTLPDEKAELAFVTQFRE
                     LLRLRNILTTFADFNTDDLALAAQPFEDYKSKYLDIHDKVRKRQAVDKVSILDDVDFE
                     LSLIHRDEINVAYILNLLVSLNKLAPQEAKKRQKEIIDLVAGEVQLRSKRALIEAFMQ
                     ENLPKLQPNDNVIQAFESYWADRKQQAFSELCSDENLIPPALEKLLSHYAFANRLPRD
                     QEIVDALNFKPKILERKPVVQRVAEKIKSFIDTFIEGMGGSV"
BASE COUNT         6666 a         5547 c         4273 g         6330 t
ORIGIN      
        1 tatcgtcccc acattcacat gcggcttcaa tcgctcaaat ttactcttcg ccataattca
       61 gttctaccct ataatttaat aatctatgaa aatcaaaatt atttattctt attaataatc
      121 gcctcagcca cacttttagg cgcttcagaa taatgtttaa attccattgt ataagttgcc
      181 ctaccttgtg tagcagatct taatactgtc gagtatccaa acatttcagc cagaggaact
      241 tcagctttaa taattttaaa accaactgta tcatccattc cttgaatcat gccacgtcta
      301 gaagacaaat cacctaccac gttacccata aagtctgggg gagtttctac ttcgactgac
      361 ataatcggct ccaaaagaat cggggttgct tttttcatac catctttaaa tgcgattgaa
      421 gccgccatct taaaagcatt ctcatttgaa tctacatcat gatatgaccc atcaaacaac
      481 gctattttta catccaccac gggataacca gccaatacgc cattagtaag agagtctatc
      541 aatcccttct caacagcagg gatgtactct cttggcacca cccccccctt tatctcatca
      601 ataaattgaa aacccttccc tgcttcattt ggctccattc ttaaccaaac atggccatac
      661 tgtcctcgcc cgccactttg cttgataaat ttaccttcaa tctctacttt ctttttaatt
      721 gcttccctat acgccacttg cggagcacca acattagcct ccacactaaa ttcccttttc
      781 atacgatcta caataatttc caaatggagc tcacccattc cagatataat tgtctgacct
      841 gactcctcat cggttctaac tctaaacgaa ggatcttctt gtgccaatct attaagcgct
      901 atgcccattt tctcttgatc gttttttgtt tttggctcaa ccgcaacatg aataacaggc
      961 tcaggaaaat ccattctttc cagcgttatt atatgctccg tatcacatag tgtatcgcct
     1021 gtgattactt ctttcaaacc gactgcagca gcaatgtcac ccgcacgcac ttcttttatc
     1081 tcatctcttt gattagcatg catttgaagc aatcgaccaa ctctttcttt tttctgctta
     1141 actgagttat atacagtatc accagaattt acaactcccg aataaacacg aaagaaaatc
     1201 aattgcccaa cataaggatc agtcgctatc ttaaaagcca gactagaaaa tggctcttca
     1261 tcacttgcat gacgctcatc cttttctcca tttgatttta atccctgtat tgccggctta
     1321 tctagcggtg atggtaaaaa atcgattacc gcatcaagca ttgcttgaac gcccttattc
     1381 ttaaatgccg agccgcaaag catgggcaca atttcgtttg caagcgtcct aatccttatt
     1441 ccatttttta tgtcttccgc agccaattct ccactttcta gatacttatt cattagctct
     1501 tccgaagcct ctgcggcaga ctctatcatt gattctctcg cagcctttac agaatctagc
     1561 atatcctcag gaacatcacg ctcatcatat ctcattcctt gagaggtttc atcccaatag
     1621 atcgccttta attttattaa atccacaatc ccttcaaatt tatcctcagc accaattggc
     1681 aattgaatcg gcactggcaa cgccttaagt ctcgatttaa tttgctcctg aactcgatta
     1741 aaattagcac ccgatcgatc cattttattt acaaacgcca atcttggtac cttatattta
     1801 tttgcttgac gccaaaccgt ctctgtttgc ggctgaaccc cacccaccgc acaaaaaact
     1861 gtacaagccc catccaaaac ccttagcgaa cgctccacct caattgtaaa atctacgtga
     1921 ccaggagtgt ctataatgtt aatgcgatgc tctggataat ttttggccat cccgctccaa
     1981 aagcatgtcg ttgctgcaga cgtaatcgtt atacctcttt cctgctcttg ctccatccaa
     2041 tccatagtcg cagccccatc atgcacctca cccaatttgt gagacactcc ggtgtaaaac
     2101 aaaacccgct ccgtagttgt tgtttttccg gcatctatat gagccataat gccaatatta
     2161 cgataacgct ctataggtgt ttttctagac acattaaacc ttactaactt taattaaact
     2221 gaattctcta gcgctttcta aaaaatctta aaaacctcta gaatcgataa tgtgagaatg
     2281 ctttatttga ctccgccatg cgatgcacct cttctctttt ctttattgcg ccgccacgtc
     2341 cctcggatgc ctctatcaat tctacagcca aacgcaaatc cattgatttc tcatttcttt
     2401 tacgggcagc atctctaagc cacctcatag ccagcgcatt acggcgcacc gatctgacct
     2461 caactggcac ctgataattt gcaccaccta ccctacgact tttaacctca acaatcggtc
     2521 ttatattgcc caatgcaatt gtaaaaactt ctacaggatt tttacccgtc tttttctcta
     2581 cttgcgaaag tgcaccataa ataattcttt cagccacaga ctttttgccg cgagtcatca
     2641 aaacattcac aaatttagcc acttcaatat tgctatactt tggatcaggc aaaatttctc
     2701 gctttggtat ttctctacgc cttgacattt acctaccttg aaaaaaattc aaataaaatc
     2761 aaccaaattc cgactaagat actttaggtt tttttgaacc atacttagat cgaccttgct
     2821 ttctatcttt cactcccgcc gtatccagac taccgcgaac cgtatgatat cttacaccag
     2881 gaagatcctt tacacgccca ccccgtatca acaccacaga atgctcttgc agattatgac
     2941 cttctccacc aatatagctc gacacctcga agccatttgt taatctcact ctagcaactt
     3001 tcctcaaagc agaatttggc tttttaggtg ttgttgtata tacacgtgta cacacacccc
     3061 ttttttgcgg gcaactttcc aaagcgggaa ccttactctt aactctttta tcttgccgtg
     3121 gctttctaat taattgactt gttgttggca ttttataatc tatgacttac atctttcatt
     3181 taacttattt aactagctag cttgcttaac tcgtagctta ctagccacat aattttcata
     3241 acgcataata attgacctaa ctatacgcca ggccgataag cgcatatagc cagatacaaa
     3301 agattgggga ttctatgggg tttaaatcca tatgtcaaga atacacttca accaccccgc
     3361 ctaactccac taccgttaac gaaatcgtca gtcgctcagg tatgcatggc caaactagca
     3421 ccaatttaat agcctgcata atcttttcaa acccaccttc agactgtgat actgaataaa
     3481 aaggtttact actaaaccta ctttcatttg ataccgaaag taacaaccta ccttttaatg
     3541 tactgtcttc tagaaagata ctgatgaaat ccattaactc taattcttgc ccgaatccgc
     3601 ccaaaactaa atcatctcta atgtatttgc caccaactcc atcacaacta ggcatagcca
     3661 agttaaattc tgtcttgagg taaccctcta ctcgggactt agaagtatac gttatcagca
     3721 attgattttc aattaattca atctttttat tgattttata accatcgaag tctgctttaa
     3781 attttatccc gtgatcatgt tttgattcaa attggtacat cactaagtga tcatcgatag
     3841 attcaacaaa aagtgcttgc gctgctctgc ccccttctaa atccgctgag ctaatttgat
     3901 gtttaaaact caaccgatca tgtgccgatg caatacccga tccactttgt tgtaaattag
     3961 gcatatcata tttaattttg tgataataat gctcttcctg cctacgaagg gtatcaccga
     4021 agttgtgctt aagcttataa gcgtcaaatt cacaaatact agctgcccca tcaagctttg
     4081 aaatcacttg caatattcca ttattcaaat agatctcttc tactccatcc aaatctgtat
     4141 cttctatgta gatagtcggt cttggtgcaa ttagatccaa catcccttca agctcaacga
     4201 tattattgaa aacagcacgc ctaagatgtg gaagatataa accaccaaat aaaccatgcc
     4261 aatatgcatc attggcttgt gaggcataga gcttctcgcg cattgttgcg ctttgctgtt
     4321 taaccggaag cgaggcaaaa cgctgtgata aactcagcat ccgcttatgc atccaattcg
     4381 attcaggata ccgtgagaaa aaattcttcc agataccccc ccttagaaag ggtttattgt
     4441 gttcatacga tcctgatact ttagattgct gaactaaatc agcataccga ttcgctaaat
     4501 ctgcaggcaa agtccactca ttcatctcga tataagaagt tgttggaaga taaatgattc
     4561 ctcgagattt ttctgaacta tgataatccc gataatgact tgtcattatt tgaggagaag
     4621 caagtacccc ctgtataaac tgttctaacc accctttttc ataaacccat tggtaggttt
     4681 caggccaaat tccaaatttt tcaatatcat caaaatagat tgctgcaact cgttgacttg
     4741 ccgcttgatg ttccgagagt gactctatat atgcaattgc atcactcgca ggagaaaaag
     4801 gaagtttgta gcgtaacgct tcagaaatcg gaaaaagatc cagtctctga ttatcttctt
     4861 ctgtcgtaaa atagccattg agctcagctt tcgtttttcc ggcacaaaga aaatggtaat
     4921 catctacgat gacatagctt attccacaat caactaatgc aggaataaca gttgactccc
     4981 aaactctctc tgttagccat gctccctggg gccgctgccc taatttttta tttaattttt
     5041 tagagaatgt ttctagctgt ccgattcgat cgcgattggg tattgttgct agaacaggct
     5101 ccgtatcacc tgcgctaaac aactcaactt gacctcgctt taccattgct ttgagcaatt
     5161 tcatatcctc aggatgatga tcgaataaat agtcgagtaa ccacccagaa aagtgaactg
     5221 caaatttaaa atcaggatat cgatgtacta catgcaaaaa aggtttatag cagcgaatgt
     5281 gcgcgtcttc taagacctct ggaaagtttc caacaggctg gtgggcatga acaccaaaaa
     5341 gcaatgatac gggttttgac atgactcaat ttccctcacg aagggtagaa taattacacg
     5401 cgtctggata gactcatact caattctgac ataaccaaat tctgagtaat tactcaacaa
     5461 aaaaatgatt taatttgaac gtcgcatcgt ttctatcatg cgagcattac tgttgccata
     5521 gctaatgggt tctgataatt cactcggtat tggttgttta agtaggctat atagattcgc
     5581 aagattcttg cgaaataggc gatcaaaact tgcaacagcc tgtgaggaat tataatcgcc
     5641 gaaccaccaa aaccaatctg aactttcgca tgatgcaagt tgccgtttag ctgctgcttg
     5701 ttcctcatcg gttaaattac ctattttcaa gaccgcatca taacttttct tagccttaca
     5761 taggatatcc caagccaaat ttttgtcatg gcttccgatc caggttgaaa aattaccata
     5821 aacccagctc cccgcggcca aattcggcag aaaaacagcc tcagtatccg attcttttaa
     5881 atgcactaag tagtctcgaa acgtggaagt tttaattgcc aaatgattcc ctatcatttc
     5941 gtaaagatcg tttagaaagt agtatccatt ataaggatag gattcccatg cattttctcc
     6001 atctagtatg atcgtaacca cgggatcctc agcatccttt acctcatgat gaatcctttc
     6061 cagtgaatgt acaaaatttt ctgcagcatc ccgaccatac catttagcat actcaaatcc
     6121 gatcatatcc gataacttgt catcacgaaa aaaacaaatc acatcttctt tatttgttaa
     6181 tactttataa gcgcgatata aatactcacc ccgctcaggt aactgcctat caggatagga
     6241 tttacgcaag ctctgagcca aaaccccttc accactcgca gtccatttca actccgatgc
     6301 agcaagaatt tctacgaaat cctttgaaat cccaccttct gcaggccata caccaaatgg
     6361 gtaagaatga aagcgttgtt tatgagattc tttagccaat tcaagatgat aagcaacacg
     6421 gtctcgtcct ccaggataat cagaacttga tggtaaaaca gcatcaggaa ggctatcgta
     6481 ggctgaattg aaatcgatca ataagggtga aagtgggtga taatgtgggg tagttgataa
     6541 ctcgatctgt cctgatactg caagtttgcg atagcgcgat atcaaatcct gaattaatgt
     6601 tccgatcaat tcaaataact gcttgcgatc actataggta aaacttttac cctgctgcat
     6661 cagttttcca actaattcac aatttttgcg aacactctca cccgtccagg ctaggtgata
     6721 ccaagtcaac aaatcagcaa gatactgacc tgaaacgtaa ctcatctctg cgtccccgtt
     6781 gcctatcaac gtttcataga tatcccgtaa atgctgatag gctcggtagg gtttgagcat
     6841 cgtatcatga ttactttgaa aacactgatt caaaataaac attcgatcct ctagtgaaat
     6901 caagttcaaa tctggagtaa tcaataagcg tagtagtgga tcccgcagcg tttcttgcaa
     6961 aaattgatca gagtaatctt caagctgttc aagaagtata ggtacaaaat tgatcactgc
     7021 ctttactctt ggatgtttct ctaagtgata ggccatatcg gtgtaatctt tgatcgcgtg
     7081 caagtaaacc cacggaagca aaaattcacc agtttcatgg tcccgatagt caggctggtg
     7141 catatgccac attaaaatta aattaagcga attcattccc aaacgatcca acaaaaaatt
     7201 taaaaattcc tcacttaacg taattcatga ataccctgcc caagcatttc aggcgtaatt
     7261 aacgtaattc cttttttagt tacatgaaac cgcttaccat cctcttcagc gtcaaaccct
     7321 accctaagcc cttctggaat gatgcagttt ttttctacaa caacattttt taaacgtgcg
     7381 tgtcgtccaa tatcgacgtt aggcagtatc accgaatctt caattattcc aaaacttcta
     7441 acctgaacat tggaaaacaa taaagagcgg cgaatcgtcg caccactaat aatacagccg
     7501 cccgacacca tggaatccag cgcttgccca cgccgattat catcatcaaa aacaaacttg
     7561 gcgggcggca gttgctcttg gtatgtccag ataggccagt cctcatcata aagattaaga
     7621 tcgggagtca cggttgtcag atcgatattt gcttcccaat aagcatcaat cgttcccaca
     7681 tccctccaat aaggaatacc tgatgccatg ttgacacaac tattggtaaa gcgatgcgca
     7741 tacactcgat attggctatt gactaaataa gggataatat cttttccaaa atcatgcgtt
     7801 gaagcattgt catcatgatc ccgtatcaat tgctcaaata aaaactgtga attaaaaaca
     7861 taaatgccca tactagcgag cgcacggtct ggctgccctg gaatcgcggt agggttctct
     7921 ggtttctctg caaagctcgt tatgcgccat tgctcatcaa ccgccataac tccaaacgct
     7981 cgcgcttctt caaacgaaac ctccaaacac gcgatagaca tatccgcgcc tttttgaata
     8041 tgctcgtcca gcagcttgct gtaatccatc ttataaatat gatcgccacc taaaatcaat
     8101 acatagctag gatcatgatt gcgcaatata tccaaattct gaaacaccgc atcggcagtc
     8161 ccttgatacc aagtttcttc tgttcgttgt tgagcaggca ataattcaac aaattcctta
     8221 aagcgaccat caagaaaact ccaccctctt tggatatggc gaattaaact ttgcgcttta
     8281 tactgagtta cgaccccaat ccgacgaaca ccggaattca cgcaattaga aagtggaaaa
     8341 tcaatgatac gaaacttgcc accaaagggc acagccggtt tggctcgcca atcagtcatg
     8401 ttatgcaagc gactgccgcg gccacctgcc aaaattagtg caagggtatt tcgtgtcgcc
     8461 atacaacatt cccccgataa aaaagtttac tttctaaccc tgaattacta tcccaatgat
     8521 ttataaacaa aaaagtgcgt ctttgaaatc taaaatcagg tatcactttc ccatcgccac
     8581 aggctgattt aaactgtggc tgttcatcga attgtttatt cttctattgt tccatcttac
     8641 ttgatcccta atagcattac tagccatgaa atcaaagcag ctatcaaccg tttcttaccc
     8701 tctacagcat gagctgctta cagctagact tcatgatcca tttgcttatc taggcgcgca
     8761 cccggtttcc aatgaaaccg ttgtaagagc tttcttaccc catgcagaaa aagtttggct
     8821 gcaaactgaa gcaggtttta ctgaaatggc ctgtgctcat gtcgatgggc tctttgaatg
     8881 gcgcggtaaa gattcaatcc caaaatgcta tcgcatcaga atgcgtaaaa aaaatgatgc
     8941 ggcaccagaa aatttacatg aattttatga cccttactcg ttcccactgc aaatttctga
     9001 tttagattta tatttattta atgaaggtaa attacaccaa gcctaccgaa tgctaggggc
     9061 gcatcactta acgatcaatg acattgcagg aatacgcttt tcggtttggg cgcccaatgc
     9121 cgagcgagtc agtgtcgtag gagatcacaa tggctgggat gggcgtgtcc atcccatggc
     9181 cgttcatcat caaagtggtg tttgggaatt atttatacca ggatttccag gtcatgggat
     9241 ttacaaatat gaaattcgta atcgccatag cggtgagata ctactaaaaa ccgatccttt
     9301 tgcaaattat catgagattc gaccgaataa tgctgcatat acaccgactc actccagttt
     9361 tacatggagt gacgatctat ggatgaatca aagaaaacat tgggattggc ttcatgcacc
     9421 gcttaatcta tatgaaattc atgttggatc atggaaacgc caccccgatg gtaaattctt
     9481 caactatcgt gagttagcgc accatttaat tccttattta cttgaaatgg gttataccca
     9541 tgtcgagtta ttgcccattt ccgaacatcc ccttgatgaa tcgtggggct atcaaacgac
     9601 aggttacttt gcagtaacaa gccgctatgg ctcacctgag gatttcaaat tttttatcaa
     9661 tgcctgccat caggcaaaca taggcgttat tctggattgg gttcccgcgc attttccgca
     9721 agatagtttc tcactggcac gatttgatgg aactgctctc tacgagcatg aagaccctcg
     9781 gctcgggttt catcacgact ggggtacttt tatcttcaat tatggtcgga atgaagtgaa
     9841 atcgttttta ctatccagtg cgcattactg gttaaataat tttcatctcg atggattacg
     9901 ggtagatgcc gtggcctcaa tgctttatct cgattactcc cgtcaacacg gcgaatggct
     9961 accgaataaa tatggcggtc gtgaaaatct cgaagccatc gatttcctgc gcgagctcaa
    10021 tatcatggtt catgatgcat ttccgggcgc actcacattt gcagaggaat caactgcatg
    10081 gcctgcggtt tcacgtccaa cttatgtggg aggactcggt ttctcgatga agtggaatat
    10141 gggctggatg aatgatacgt tgagctacat tcgccaggat ccgatttatc ggcgctatca
    10201 tcataatcag cttaccttca atcaactcta cgcttattct gagaactttg tattaccctt
    10261 ttctcacgat gaagtcgttc atggaaaagg gtctttatta ggtaagatgc ccggtgatgc
    10321 ttggcagaag tttgcaaatt taaggctatt attaacctac caaatgactt gcccaggaaa
    10381 aaaactaaac ttcatgggca atgaatttgc acaagcccgt gaatggcaag tcaatcaaca
    10441 actcgactgg aacctactcg attacaagct gcatcgcggc gtacaattag ccatacatga
    10501 cttaaatcat ctataccttg aaacacccgc acttcatcaa ctcgattttg aacaagcagg
    10561 tttttgctgg gcagactgta atgacgccga tcaatcagtc atcagttatt tgcgttatgc
    10621 gcaagatggg tcattcagcc tcatcgtcct gaatttcaca ccggttccac gggagaatta
    10681 tcggatcggt gtacccgcac ggggtattta tcatgaagtt ttcaatagcg attcaaccta
    10741 ctacggcggt ggaaatgtcg gtaatcccaa acaaatcttt tctgttgcag aaccctggtt
    10801 agaatatcaa gattccgtga tcatcacatt accgccttta gctggaatta ttttagcgct
    10861 tacccctgct tgatcattga caagttttta aatcgatatc ctctttaaac ttgatctcca
    10921 accaccatat aataaaagca actcgtactc aaatataggg ctaaccgtta acatgaccat
    10981 gcaagcgaat acaatcgatt ttgaaaacta cgaattacta caagatgaaa tttgtgcgca
    11041 aagaatcgtt gcagccaaag ccaagctagg gaaaaaggcc gttattctgg cgcatcatta
    11101 ccaacgcgca gatgtttact gtcatgctga tttaacgggc gattcgctca agctttccta
    11161 cttggctgcc caaaccgatg cagattattt ggtattctgt ggcgtacact tcatggcaga
    11221 agtagcagat attctctcaa gcccgcaaca aatagcgatc ttacctgatc tctcagcggg
    11281 atgctcaatg gcagatatgg caaacctttc caaagtagag cgcgcctggc gtgagctttc
    11341 agaaattctt aatcctgatg aaaaaatcac ccctgtcact tatattaatt ccagtgctga
    11401 tctaaaggct ttctgtgctg aacacggagg catcgtttgt acgtcgagta atgcaacaaa
    11461 agtcttgcag tggtccttcg aacaacgtga aaaagttcta ttcttccccg accaacattt
    11521 gggacgttgg agtggctata aaatgaacat acccttggat gagatgttga tatgggattt
    11581 tgatgaaccg atgggaggat tgtctgaagc gcagatcaaa aaagcaaaaa ttttattatg
    11641 gaaaggacac tgctcagttc atcaaatgtt tcaacctcag cacattatcc gtttccgcaa
    11701 ccaatatcca gatggtatcg taatctcaca tccggaatgt agctttgaag tctgtaaagc
    11761 atcggattat gtcggttcaa cagaatacat catcaaaacc attcgcgaag cagcgcctgg
    11821 aacaagatgg ttagtaggca cagaattaaa tctagtcgga cgaatcgccg aagaatttaa
    11881 agcacaagga aaaatcgtgc agttcatgtc gcccatggtt tgcatgtgtt caaccatggc
    11941 tcgaattgat ccacaacact tggcttggtc actagaaaat ctggtggccg ggaaaatcgt
    12001 aaaccaaatc aaagtacccg atcaggaagc gcaattagct aaactggcgc ttgaaagaat
    12061 gctagcgatc tccaaatagg aggaagtgaa tcatcttttg gatactgcgg cgctttgtgt
    12121 tctggaaaat tgattgatta agccaattta tgaccaacac cttacacatc gcaacgtata
    12181 atattcacaa gggattttcc ttgttcaatc agcgcattgt cttacatgaa atgcgggaaa
    12241 aactaagaac gctaggtact gatatcattt ttcttcaaga agtggtggga gcgcatcgca
    12301 agcacgccat gcgctttagc aattggcctt cgaaccctca atatgaattt ctagcggatt
    12361 ccgtgtggcg cgattttgct tacggtaaga acgctgttta cgatgaaggt caccatggca
    12421 atgctattct cagtcgattt cccattatcc gttgggaaaa tatcgatatt tccgcacatc
    12481 gttttgagca acgtggctta ctccactgcg aaatggatat tccaggatgg caagaaccac
    12541 tccactgtat ttgtgtgcat ttaggtctat tcaagcgtgg ccaagcaatg caactgcgtg
    12601 ccattgaaaa acggttgact gaaatcatcc ccccaacagc cccactcgtt attgctggtg
    12661 attttaatga ttggcacggc tctgcaaaac gtattttgac cgaatcgatg aagctagtag
    12721 aagtttttga acagacccat ggcagaacag cgcgaagctt cccttcccta tttccattat
    12781 tgcgcttaga ccgtatctat gtcaggggtt tccaagtaaa aaatgccgtc gttcaccaaa
    12841 gcccaacgtg ggctagaata tccgatcacg cagcacttac tgcaaatatc gttcgtcgat
    12901 gaacaaccct gattttattg atcacaataa aatcatatta ctaaacaatg gttctcagta
    12961 cttccccgct ttagtatcag ctattgagca agccaaatac gaaatccatc tccaaactta
    13021 tatttttaag tacgatgcca caggaatcat catatcagaa gcactaaccc gggcagccaa
    13081 acgaggcgta gccgttcatt tactcatgga tggatttgga tcacttgatt tccccaacaa
    13141 aatcatagag aagctaatca cggaaggggt tcaagtgcta atctaccgta aggaattgct
    13201 tacctttaag ttcttaaaac atcgattacg gcgtatgcat cgcaaagtcg tggtaatcga
    13261 tgctcaaatt gcctttatcg gcggcatcaa tatcatcgat gatcatgacc ctagggcgag
    13321 tcagccgtta ccccggttcg atcacgcagt cagaatagaa ggcccactat tggcaaatat
    13381 ttatcctgtg gtgaaacgat tatggatgct cgttgcgtgg gtacacttca aaaaacgatg
    13441 gattaagaag ctgccagaat taaaaccaca actcaaatca agcggtaatc agcgtgccgc
    13501 actggtcatt cgcgataata tccgccatcg acgtgatatc gagcatgctt acttaaaagc
    13561 aatcgccacc gcagaagaag aaattattat cgctaatgct tatttcttac caggaagaaa
    13621 ttttagaaag gcgttgatcc acgcagcccg acgaggtgtc agggttatat tattgcttca
    13681 agggaaaatt gaataccgta tccaacacta tgcctcgcgt gcactatatg gaaatttgct
    13741 tgaggcgggt atcaaaatct atgaatatca caagagttat ttacacacaa aagtagctgt
    13801 catcgataaa tactggtcaa ccgtcggttc atccaacatt gaccccttta gtctgctgct
    13861 agctcgggaa gccaatatta tcgttactga tcatgcattt tcttcagaac tacgcgccaa
    13921 cttacagcaa gcactgcacg aatccaatcc ggtttctgca agatattgga aaaaacgcgc
    13981 ttggctcgat cgactgctaa actggattag ctattacaca ctaagatttt tgcaaggaac
    14041 gctcggttac gggcaaaaga gtacacgtat ttaattgcag aaaattattt ttcttgaatc
    14101 gttgatgaag ccgataataa atctagaatc ggtatatcga tacgaccgat gagtggtaac
    14161 ttaatcgcaa atggatcggg gtcaatccca aacgttaacc ccaagatatt gagttcaaat
    14221 ccttcaaccc gactcacagt cactccaagc agcccaaaca ttgaccactg ccagccatga
    14281 ccgctgggcg ctgttgctac ccattctttc cctagaaaat ctttgccaat tgctgtcggt
    14341 ggtaagtcca aatctagttc aggcacagca cgtgaaatcc aagcagtgaa ggtattcgaa
    14401 ttagggccag gccatatcca atactcgtaa tgatagggat attggtaagc tgcttgctca
    14461 acccgctcaa tcagcgcatc aaccccctct ccacgttttt caataagcaa ctgtggcata
    14521 tttccaaacc agcggcgatc cggtatttgc tcatgaatgg caacaaccga accccctcgt
    14581 cttaaatacc aaccgattac ttcataaacg gtataagcat tcgcattcga tggtttgacc
    14641 gcaatccagg tatgaactcc aaaataccca cgccaaccat atgcacgggc accataaacc
    14701 tgaattaatg cttccggagt ttgttccgga cttggagcta atccaaccga ctcacgactc
    14761 gctgtgcgcc agcttgcgtt aacgtgctcg gtaagataat gcgtgagtaa cagaatgacc
    14821 aacgctacta acagaacagg tttaaccact tgtcagtgtg atccgtgatt aaatcgatcc
    14881 aggtaacaaa acgaactccc taagaaaacg ggattatcgc gctgaaacca agatcgaagc
    14941 gcggcttacg acctcagctt gataaggatg cgttactttc ttatagcgcc ctctaatttt
    15001 ttccacataa ttcagtgttt cccgataagg tggaacgccc cggtacttct caaccgcgcc
    15061 ttccccagca ttataggccg cgactgcaag cgtcacgtca cctttgaaga aggctaacag
    15121 ccagcgtaag taggccatcc cgcccttgat attttcctct ggatcaaatg tatcctttac
    15181 ttgaaagcgc tgtgccgtcg ctggtatcaa ttgcatcaat ccttgcgcat tcttaggaga
    15241 aatcgcttta accttgaatg cggactcaat cgagataagt gccaaaacga gtttaggatc
    15301 taattgataa tcaggcgcta atttatgaac caaatgaagt acagggccct ctggataggt
    15361 gtaatcactg tcttcttcat catcaaaaat ttcaatgctc ggaatcaagc aagcgggaag
    15421 cgaaaccggt aacatggccg gtgtatactg aatcattttc tgtgcataat gatgtccctg
    15481 ttctgcggcc atctcaaaca attgtgcggc gatactatca tcgcgttcta caccccgccc
    15541 attcgcgtac atccaaccca gtgcaaattg cgcgtctgcc gatcctaagc gtgccgcttc
    15601 gcaatataac tctgctgctt tggttttatc ttcaggaaaa ccttcagcat gttcatactt
    15661 aactgctagc gcaaataatt cattaggatc tttatcgata accggcaagt catctaaaaa
    15721 tagctctgat gaactttggt tggcagaaac tgcggtaaca actaatgaca aaatagtgag
    15781 taatattagt tttcgcataa aaaatgattt ttctgacaaa ttccgaataa acgcctgcac
    15841 agttgaactt aatcgaaaga atctgaccgc tcttactaaa agtagacaat ggataaatac
    15901 caaaaagtcc aatacgatcg aatgttgatt ttactgcgtc ggctactgaa cagcaaaacc
    15961 tgggctgttt ggttttgtta ctaatacttt ctttcccatt actaaagcag ctactatgcc
    16021 agcgataatg aatgctttta aatcagcaag ataacagcac gacccggagc caacttaaaa
    16081 aaagtgataa attttccgac acttgcttta tgttaaacca aaccacatag gatttgtcat
    16141 taaacgacaa attaccaaag atatcgctgt gaaaaatcac agcaacgaag aattgttagg
    16201 tcttgcttcc ttccttatcg ctagcctcag ttggccagat agtaaaggga aaaggacgtt
    16261 gcccggtgtt aaacgtcaaa tacaaaacga tctgatacac gtaagctgcg agatacttcc
    16321 ccaatctgag caaatgcgtg tttgttttcc ccgttaccaa aatggaaacg tactgaaaaa
    16381 acataatcat gataatcaaa gattcaacaa caccagaaac aaaaacaaaa aacaagataa
    16441 aaaaaccttg tacccaggtc tctgttctct gcaatcgttg cttcacttct tctttcattc
    16501 tttgcccagg aagtagcatc atatccgtac tatgcgttga gagtagacta cacagtaaat
    16561 gaatttataa agattagcaa aactctttat agatcagtgt gaacaaaaaa caaccaaact
    16621 agtgctagcc aatcaacccc tcaataagat cttagggaca gcccttacat cccttagtac
    16681 gcgccatggt aaagatagct tgatcgagat tggcaccact cagatcggta ttactcaaat
    16741 cagcgccagt aaaatcaacg ccttgcaaat tggcattcac caaggatgcg ttttgtaaat
    16801 gggcagaacg cagattcgca cgctgaatat tcgcattatt taatgatgcc ccaaccagtt
    16861 tggcgttagt caaatccgca ctcgttagaa cggtatccgt taaatttgcg ccactaaaat
    16921 cagcattttg tagaatcgca cttcttagat ttgctcccac caattgcgca tcattaaatc
    16981 gtgctcttct tgcattcgtt ttgattagcg aagcttgatt tagaatcgtt ccgcgtaatt
    17041 gcgcatcaga taagtttgct tcatcgagca ccgccttggt cagattggcg ccagataata
    17101 tggaacgggt aaattgtgaa ttcgccagat tggtattctg cagatcggcg ccatttagac
    17161 tcatatcaac gcaaaacgta ccacgtcgaa tactacaccc ttcaacattg cgcgcgcttt
    17221 gttgcgcaag cacctccgta gcgagaaatg ggagtagcaa cagcccacca ctaaggatgc
    17281 tagtacataa gcgcaaacaa tcattcagca ataaacttag gcgaatcgat aatgaatcga
    17341 gattgctaat taacataagc gagcgcaact ccataaaaac gttttgaaat taatcggcaa
    17401 agataattta agcggactgc ctaccacagc tattcttact cggctgggca aatgtactga
    17461 ggatttgagg ggatgtcaaa cctttcatct cggctagcgc atcgcttctg gcaaatcaat
    17521 cctgaaatca caaaacccat caattcactt cacttttagc gatatacttc atagtaccgt
    17581 aacccttgcc gattcgttaa tccccattta tttccaatga gaacatcgct aaaacccatt
    17641 aaaggtattt tgatttacac ccttatttta ttcaccgtat cgctaatata cttcatctat
    17701 gcgttttcgg tttatcccag tagtgaagaa caagaaactt atctccatga gattggtgaa
    17761 ggatttggaa aaacagggct agcattactc ggtttgatct attttcgtac tttcttaaaa
    17821 ctcctcctgg gaaaaggaaa gttagcacaa cgcttacttc cagagtatca accgccattt
    17881 gatgcaaact tgtttgatca actactcggg tttctgaatc gcacccatgt ctatgtcggg
    17941 attgcagcgg tagcaattct cttattgcat gctgccatga tgggattgac gcaacatttg
    18001 catattctct ttttcccagc gctgctggct ttaatcatct ggcaggcact attcgggcta
    18061 tttctcacgt ggcgttatag caccaccgaa ttaaagcaat tttcatatct ggtccacgcc
    18121 caatttgtaa ctggcatcgc catcggtatt tttgcgtttt ttggacatat tctgatcgat
    18181 gactaagcgg taaaaattgc tcagtaagca gcgtggcaaa aacccaacac ttatcatcgt
    18241 tctaagctaa ctcattttgt tcatcgctta aaaagtgcta taaatcgaac tagatgtgca
    18301 gcaagctgcg atcatttcta agcatcaaca gaatccaatc gaataggggc gcctaatatg
    18361 aataaaaata aacgcgtagc tagcctattg ctacttataa ccttaatacc ctttctatcc
    18421 ggctgttgga cattagttgc ggctggtgca ggtgcctatg gcggctacaa gatgaaagag
    18481 aaaggctaca ccctgcaaaa tccgattacg aaggaaaaaa acaaaaaaga ctaattctga
    18541 ataataacct cgtagagttt tcccatgttt tccttatcat tacgcttctc actcattacg
    18601 agcttaatgg cgatcttgct gttgagcggt tgccaatctt tgtttatgag tagcgcgccg
    18661 cttcctgcac ccgttgctaa gccactgccg gttgccatag cgagtcacga atttagcttt
    18721 gaccctacac aagaggatgt cgtcgggacg ttacaaatca caacggtcaa tgaagaagat
    18781 acactttccg atattgcccg tcgcttcaat ttgggctatg aagaaatcgt cagcgcaaac
    18841 ccaggtgtcg atccttggat accgaaagtc gggactccta tcgtacttcc gacccaattc
    18901 atactccccg agggtccacg ccgtggtatt gtcatcaatc tcgcggccat gcgcctgttt
    18961 tacttccctc ctaagtccaa aaatagcaag ctgcagaaag tcatcaccca ccccttaggc
    19021 atcggcagag tggaatggaa aacaccgcaa ggaatgacct cgatcatcgc taagaaagaa
    19081 aacccggctt ggataccaac cgcctccatt cgaaaagagc acgctgccaa tggcaacccg
    19141 ctcccagcaa tcgttccgcc gggcccagac aatccaatgg gaacccacgt actaaggcta
    19201 ggctggacta actacgcgat tcatggcaca gataaaccgc ccagtatcgg tctgcgaggg
    19261 agtcatggtt gtctccgcat gtatccagag gatataaccg gtatctatga tgccgtaccg
    19321 gttggaacac cagtgcgcgt cgtcaatcaa cctcatttgt taggatggcg cggcgatcat
    19381 ttatatttgc aaacctaccc cattctggaa gacgacaaac gcaatcacaa gcagcgactt
    19441 cgtcagtcat tagcgaacat acgttcaacc tccaaagcta agcttggggt acgcccacaa
    19501 aataaaattg atcccgtact ccttaaagaa atcattcaaa gtgagcgcac cgtctctttg
    19561 ccaattacgc aggcacccac tccactactc acgttactcg atcagcacgc gctcaaggtc
    19621 caaaatacgt taccgcgcta cgcaacttgg agtggcgatt caagccagca actaacggtt
    19681 gcggaaattg taaaaatggc cgaaggcgaa ccttaatcga tcgttggaca ttatccatta
    19741 gcgtattggc gtgacaacat cacaatgctc accgacccag cgcccagacg tctcagcatg
    19801 ggcaacaatc ggtgaacctt gaatcacgct atcaaactca gcttgcccag aaaagctttc
    19861 tggggtcatg aacacacctt ctgcataacc ctcgccctta aactgggcat tactgcaagt
    19921 aagctccatc tgataacgat tcccagtgcg ggaagcattt ttaacggtgc atcctgtctg
    19981 gccctcaaag aaataagaag gcacttcttg ttttgccatt tccggcgtaa tacacatttt
    20041 tgagttagca gcaccattac tgatctgggg catttctagg ccatactgct tggccaggcg
    20101 ggtaatttgc tgcatttgct ctgcaggaac atgcggcacc aactgaagta aatcagattt
    20161 cgttgtaacc tcccacaatc caggtcgaat gatgctttca gcaaaaactg agggtaacgc
    20221 agcaactgaa cacccaatca ataacataac ctttcgcata acatcctctt taataaatat
    20281 actgataaaa agcaatctac ctggctaata ttagaaacta ccatcttaac agagctgctc
    20341 taatgctttc tttagaactc gccattagtg tccagttcta taaagctcta aattttaaag
    20401 cccgagaaac gatggctcgc tcacagaact aaaaacagca cgttatgtcg aaatttaagt
    20461 catcaagcat gaaaggtaaa acaatgaaaa caatccttac agcagccata ttattcatta
    20521 tgataatcac ctccctcgcc attggaaaaa ccattacaca aaattccgga aaatcgaatc
    20581 aaatcatgaa ttcaactaca gattatattg tcttcggtat gggttgcttc tggggtgctg
    20641 agaagcgcat gagcgaaatt cccggtgtca tcgatgtgga aagcggctat gccggcggcg
    20701 atatcgtcaa tgcaaactat cagcaaattc tagcccatga aaaagcgtta cgtgctggaa
    20761 aagctgaagc tcggaaccac gctgaagtca tcaaagtgac gttcgatcca gaaaaagtat
    20821 cgctggaaac cgtgctggca aaattctggg aaaatcataa ccccacccaa ggcgatcgcc
    20881 agggcaatga catcggcagc aattaccgca gcgcgattta ctaccacaac cctgctcagc
    20941 tagcccaggc acaagttact cagcaagtct accagcacgc gcttactgcc gcaggatatg
    21001 gcgccatcac cacggaaatc ttgcctttaa aaaattacct gacagcggaa gaatatcatc
    21061 agaattattt gcaaaagaat cctaatggct actgtgggtt gggcggtgtc ggcgtaaagt
    21121 acccgcaagc aaaccaaacc ctgagccaaa atcctaacgc aacagatgcc aaaattgctg
    21181 aactcctgaa accgctcaaa ggcagcgagc taaaccacgc gcaacaactg gttattttcg
    21241 aagcggagaa ctgtgaatac tgcaagctgt tcgatcaaga tattcttaac tactggcaag
    21301 cggaagtccc cgtcgtcgca accctaaact ccaatcctcc cgaaggctgg acattggaaa
    21361 aacccctgtt cgcagcacct accatcgttt tatttcgcaa tggccaagaa gtcgcgcgct
    21421 acaccggcta taacggggaa atcaccaatt tctggaaatg gctcggattc cagctattat
    21481 cccccgagca acaaaaaatt gcgttccagc acggcacgga accgcccttt accgcgccta
    21541 atctcgatga aaaaagaccg gggcgctttg tcgatcccat tacgggcgcc accctatttc
    21601 tgagcaaaac gaaatttgac agtaaaaccg gttggccaag tttcttcgac cccgtcgaag
    21661 gatcggtaac actgcataac gacgattcgc tcggtatgcg ccgaatcgaa gtccgcagcg
    21721 caagttccgg catccatctc ggtcatgtgt tcaacgatgg ccctgcaccc agctataaac
    21781 gctattgcat taatggcaac gtattgaaat tcataccgga ctgaaaatca ggagcgtgtt
    21841 tacagtcacg ccagcaacct caaacactcc cgcccatgcc ttcgataaag gtatcgataa
    21901 acgatttgat tttttcagcc acccgttgaa caaccggttt gcgttccagg attttgggct
    21961 tgaaattcaa cgcatcgaca atttcctggt cacgcggtaa acggttggca aacgcataat
    22021 ggctgagcag tttttccagc gctggtggta tcaagttctc atcgctgcat aattcactaa
    22081 aagcctgctg tttacgatcc gcccaatagc tctcaaacgc ttgaatcaca ttatcgttag
    22141 gctgcagctt cggcagattt tcttgcatga acgcttctat caatgcgcgt ttactgcgca
    22201 attgcacttc acccgcaact agatcgatga tttctttttg ccgcttcttg gcttcttggg
    22261 gggctaattt gttgaggctc accagcaaat tcagaatata agccacattg atctcatcgc
    22321 gatgaatcag gctcagctca aaatccacat catcgaggat cgatactttg tcgaccgctt
    22381 gccgcttcct gactttatcg tggatatcca gatatttgct cttatagtct tcgaaaggct
    22441 gcgcggctag cgccaaatcg tcggtattaa aatccgcaaa agtcgtcaaa atgttacgca
    22501 agcgcagcaa ctcgcgaaac tgcgtcacaa acgccaattc cgccttttca tcgggtaaag
    22561 tatccacact ggcaacggtc ggcgcaatcg ccagcaatgc ttccgttgcc tgattaaact
    22621 gctgcacata gctttcatag ggctccagca ataccgtttc cttggcattt ttactggaaa
    22681 atagcgcaat cgcctcatcg gtcgctgctt tcagattgcg aaagcaaacg atattgccct
    22741 gcgatttctt ttcattgaga atacggttgg tgcgcgaaaa cgcttgcacc aatccatgat
    22801 atttgaggtt tttatc
//
LOCUS       DAOQLJ010000018        22463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_790_length_22463_cov_5.756426, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000018 DAOQLJ010000000
VERSION     DAOQLJ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 22463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22463
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_790_length_22463_cov_5.756426"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PK522_02545"
     CDS             <1..579
                     /locus_tag="PK522_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNC40646.1"
                     /translation="ISSSSSQIILSPENPGRFILVPRHSSEIRNFIPIGFLDAFYIAA
                     DSCLFIPNATLYHFGILTSTMHNAWMRTVCGRLKSDYRYSAGIVYNNFPWPEPSDTQR
                     KTIETAAQAVLDARAQFPSSTLADLYDPLAMPPELVRAHQTLDRAVDAAYGKKSFASE
                     AERVAFLFERYQAIMSTLPIVSAKKGRKKRGK"
     gene            complement(746..1528)
                     /locus_tag="PK522_02550"
     CDS             complement(746..1528)
                     /locus_tag="PK522_02550"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNC40647.1"
                     /translation="MNKILTIGLLVSIGFTTHLAATPLHWNLNTSTDNCNNSGVGNTC
                     EFTSNTGEEIKVNAYSTNNNNGTGAFAKATLTVWSGGIGVKNPFDSNESGSPNHATDN
                     DGKDDLVVFQHYDPSYLFTGFQIGWKQNDADITAWIGGNNLGANYDFTGKHFSDLAGL
                     GFTKFTFNNVSVNTTQDLDPDTIGRYLIIAPTEFDSRTNCGPRHRSACDQYDYFKISQ
                     ISGDEPVTVPEPGTLALFGISLFGLWLSSSLRSLRLRLPALN"
     gene            complement(2048..2689)
                     /gene="pyrE"
                     /locus_tag="PK522_02555"
     CDS             complement(2048..2689)
                     /gene="pyrE"
                     /locus_tag="PK522_02555"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNC40648.1"
                     /translation="MSDYKKAFIELAIGCNVLRFGEFKTKAGRLSPYFFNAGLFNNGN
                     ALRQLGQFYAKAIIAAQLPFDMLFGPAYKGIPLVSTIAIALAELGYNYPFCFNRKELK
                     DHGEGGITIGAPLSGRVLIADDVISAGTSIGESVNLIRAESATPCGVIIALDRLEQGS
                     NKLSAVQEVNNLHHIPVISIIDLNDIVDYLKEATNMRQNLQAIQAYRERYGTQ"
     gene            2710..3483
                     /locus_tag="PK522_02560"
     CDS             2710..3483
                     /locus_tag="PK522_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNC40649.1"
                     /translation="MRIITLNVNGIRAAAKKGLFNWLPLQQADVICVQELKAGIDDMS
                     PEMKAFDGYQGYFHCAEKKGYSGVGVYTRNKPDQVIEGVGIPGIDSEGRYLRVDFGSL
                     SIVSIYLPSGSSGEHRQDAKFYFMEHFFPFLEEFSKEGREIILCGDWNIAHKEIDLKN
                     WRSNQKNSGFLPEERAWLSAVFEELKFVDVFRRLNSESEQYTWWSNRGQAWTNNVGWR
                     IDYQIATASIADKATQVSIYKQERFSDHAPLIIDYDYQL"
     gene            3480..4853
                     /locus_tag="PK522_02565"
     CDS             3480..4853
                     /locus_tag="PK522_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HNC40650.1"
                     /translation="MKNTSLIAWLDACRIYLHPRVMGMLALGFSAGLPLLLILGTLSF
                     WLREAGIDRTTIGHLSWIGLAYGFKWLWSPLVDRMPLPILTKMLGRRRAWLLLSQITI
                     VIALIGMASTDPLLNLTHMVFFALAVAFASATQDIALDAYRIEAVAIELQGAMAATYQ
                     AGYRLAMILSSAGVLWIAASLDTMPTGYDYRAWQIAYFVMAAFMGIGVLTTLIIQEPA
                     VKISQLAVENEKRVEAMIARWNLNHRLAQLLLWFYGAVIAPFRDFIVRHRYNAFLILA
                     LIAVYRISDVVMGVMSNPFYVDMGYTKDEVATVSKVYGVLMTIAGAAVGGVLIMRIGI
                     IKTLFIGAILSAVTNLLFVWLTVIGHSLPGLIFTISADNLSAGIASSAFIAYLSGLTH
                     SAYSATQYALFSSVMLLLPKFIAGFSGQFVDAYGYAWFFLSTALLGLPVLILVWLVSR
                     IDFHSSE"
     gene            5055..5909
                     /locus_tag="PK522_02570"
     CDS             5055..5909
                     /locus_tag="PK522_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNC40651.1"
                     /translation="MQSKTITGLKNWISFFNKAEIPVLKLTGNELFLLLQQEKNPSAR
                     SVANIIKRDPLMTVKLLRYFCQHKHRSLEHEVMQAEQILIMLGVDKAMKNVLPEPTVE
                     ELLREQKVEVLINLLHLVHRSHIASNYAFEWAVRLHDTHFEEIRIAALLHDISELLMW
                     CFAPTEMLRIREMQKKDKTLRSYLAQKQVLGFTLADLQSELVLRWSLPELLITLMDDH
                     NASLQRVRNVVLAVNLARHSANGWDDAALPDDYTAIGELLRMPVHDVKIIVGAKNIEP
                     VNSQQSEH"
     gene            complement(5925..6791)
                     /locus_tag="PK522_02575"
     CDS             complement(5925..6791)
                     /locus_tag="PK522_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNC40652.1"
                     /translation="MITSMTGYATATQEISSGLISMELRSVNNRYLDIQFKLPDEFRS
                     FEAGMRELLNTKIKRGKIDCRLTFQSYPDLNLPQQLNSQVIRQLLDLNNTVQSNFIAA
                     RSLSVAEILKWPGILVSDSSPTTDLQEACNNLLQITLTELVSTRLREGEKLKAILIDR
                     LTQMRQLTSALSPRIPTLIKNFQEKLILRLQEAQIDCNDDRIRQELVLFASKIDIDEE
                     LSRLNTHLDEVEQTLLKGGASGKRLDFLMQELNRESNTLGSKSLDVEVSKISVELKVL
                     IEQMREQVQNIE"
     gene            complement(6845..10621)
                     /gene="hrpA"
                     /locus_tag="PK522_02580"
     CDS             complement(6845..10621)
                     /gene="hrpA"
                     /locus_tag="PK522_02580"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HNC40653.1"
                     /translation="MSISQHLSAENLARRSNNLPKPKYSDELPICKQRHKILDAIRSN
                     SVIIVSGETGSGKTTQLPKFCLELGRGVSGLIGHTQPRRIAARSVAIQIATELSSPLD
                     HAVGYKVRFSDNINQDTYIKLMTDGILLAETQSDPYLQKYDTIIIDEAHERSLNIDFL
                     LGYLKQLLPKRADLKLIITSATIDAERFSHHFNNAPVIEVSGRTYPVEIRYHAKDNNQ
                     DEEEYDLQQSILDATDELLRTGIGDILIFLPGEREIKETSETLRKHRFNHFSDPGKLE
                     ILSLYARLSYAEQQRVFHKSNAVSRIILATNVAETSLTVPGIRYVIDTGVARINRYNH
                     RNKIEQLLVEKISQASANQRAGRCGRVMSGICIRLYAKEELETRPLYTDPEILRTSLA
                     SVILRMKSLKIGDIENFPFIQPPLPRMIADGYQSLSELGALDIHQKLTTIGWQLAKFP
                     IDPKIARMILAAKQENCLSEVLIIASVLSLADPRERPFDQQEAADRAHLPFHDERSDF
                     IGFLKLWNFFDELLKHKKSKKKLITQCHEHFISYRKMREWREIHGQLHTLVLEFGLRP
                     NQVPASYDEIHRALLAGLLSNIGFKSENEGEYLGARGIKFFIYPGSTLRKAKAKWIVA
                     AELVETTKLYARCAATIDVTWLEKIAGKLCKKHFFDPHWEKKTAQVMVYERVTLYGLM
                     IVPKRSVTYGKMNPKEAREIFIRNALVLGDYETNAQFLKHNRQLIQDIEELEHKTRRQ
                     DVLVEEQDIFTFYDKIIPDNILNGSSFESWRKQVEQDNPHLLYLSREYLMRHKADTAF
                     ETLFPESISIFDQQLPLTYRFEPGHAMDGVTVTAPLHSLNKLKANQFQDLVPGLIREK
                     ITWCFKALPKPIRRNLIPIAEHVTHFLDYRDKQKHSLSLLEALSEYIFSITGTKISSD
                     TWDTKLMPLHLQMNYRIIDDAGKELGVNRDLIELQDQFGQTAQLIFSQNNAANQLGIE
                     RDQITQWNFGQLPEEVTFIRAQKQLTGYPALVDQIDHVAIRLFDTIEVAHNHMRSGVK
                     RLLILELKDPIKLLEKKLPSTKSDSLVLSHLIDSETLKQDMFAAIYDRALIGDEPLPR
                     NEHEFSQLKQKGRIRLNSVADTLIQFIHDLALEYQAFKQKLSTVNPHHAQIKQELNNQ
                     LQALIYPNFLQQTDWVRLPHLLRYLKGMLVRLDKYPNNPARDNQHALAISVLWNQYRQ
                     RYEKHSKRGFKDPKLEEFRWQIEELRISLFAQELKTPYPVSVKRLQKLWEQVHE"
     gene            complement(10700..11170)
                     /locus_tag="PK522_02585"
     CDS             complement(10700..11170)
                     /locus_tag="PK522_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNC40654.1"
                     /translation="MDNIWFKPYTLDYLEGLRNANMGIHIGIHFSEIGPNFLKARMPV
                     DQRTTQPFGILHGGASCVLSETLGSVSAWMTIDPEKYRAVGLEINANHIRAVTKGYVI
                     GICTPLHVGRRTQVWQTDITEETTGKRVAISRLTVAIIEQGTLSNQKETVIVEK"
     gene            complement(11285..11710)
                     /locus_tag="PK522_02590"
     CDS             complement(11285..11710)
                     /locus_tag="PK522_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40655.1"
                     /translation="MNDLAAWEYFDNTWLFAGLVAILLSILLKYWKINTTSSINRSTE
                     QFILKSQKILLALAFVFLTGNLLIPTTKEANEKNEFNQPHQSNVTTPTPIGNTRNELL
                     SSPPAPDRIRSNRPTLTQPLDYNKNSIAEDRSEGIVNDY"
     gene            complement(11783..12820)
                     /gene="mltB"
                     /locus_tag="PK522_02595"
     CDS             complement(11783..12820)
                     /gene="mltB"
                     /locus_tag="PK522_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HNC40656.1"
                     /translation="MRLPIPKNKLKKDIHRLPTYYMVILVLGVCMLLWGATAIGSPTR
                     PEIKTFIDEMVAEHGFNRPKLEAIFSKVQFQPSIINLISTPSSSVPWSQYRSRFVNLQ
                     RIKNGVSFWNQHANVLQRANKEYGIPEEIIVAIIGVETSYGASTGKHRVIDALSTLAF
                     DFPKRAEFFRSELVQYLLLAQEQNFDLLGIKGSYAGAIGIPQFMPGSYRRFAVDFDGD
                     GKVDLANSAADAIGSVANYLKEYGWEMGGPIVVQSSVKADRLQEILESGIEPKHTIKQ
                     LQEASIIPLQSFENERLAALIELKDNENFQYWLGFKNFYVITRYNRSSFYAMSVHQLA
                     DAIRNARGSLR"
     gene            13121..13447
                     /gene="trxA"
                     /locus_tag="PK522_02600"
     CDS             13121..13447
                     /gene="trxA"
                     /locus_tag="PK522_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HNC40657.1"
                     /translation="MAQHIHYITDATFEAEVLQSPTPVLVDYWAEWCGPCKMIAPILD
                     EVANEYNDRLKVAKLNIDENQHIPQKYGIRGIPTLMLFKNGNIEATKVGALSKSQLTA
                     FIDSHL"
     gene            13576..14835
                     /gene="rho"
                     /locus_tag="PK522_02605"
     CDS             13576..14835
                     /gene="rho"
                     /locus_tag="PK522_02605"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNC40658.1"
                     /translation="MRLSDLKSLHVSELIKMAVANEVEGANRLRKQDLIFALLKNQAR
                     KNESIFGEGTLEILQDGFGFLRSPDTSYLAGPDDIYISPSQIRRFNLHTGDSVDGEIR
                     PPKDGERYFALVKVDKVNNEPPENSKRKILFENLTPLFPTERLVLERDIRAEENTTGR
                     IIDLIAPIGKGQRGLLVASPKSGKTVMLQHIAHSIAANHPDVILMVLLIDERPEEVTE
                     MIRSVRGEVISSTFDESAARHVQVADMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALVDTGSRMDDVIYEE
                     FKGTGNMEIHLDRRMAEKRIYPAINVNRSGTRREELLLPQEILQKIWILRKLLYPMDD
                     MDAMSFLLDKIKATKNNADFFDSMRRV"
     gene            complement(14914..15384)
                     /locus_tag="PK522_02610"
     CDS             complement(14914..15384)
                     /locus_tag="PK522_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNC40659.1"
                     /translation="MGFCVFLGLMTWYLGKPPSIRFYETKSEAPLTAPLAPGINMALD
                     MQSSVAVRLSEPLHVELFKGNVYFDIQKQATHQLEVKVGSTIIKDFGTRFSIQLHKDG
                     TGHIAVTDGHIKIHSPSGNVFQINAYEEAEFDETGISKHRLLSEKNIAPWHILR"
     gene            15825..16874
                     /locus_tag="PK522_02615"
     CDS             15825..16874
                     /locus_tag="PK522_02615"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HNC40660.1"
                     /translation="MTDIVNLLGQEADQLLAHVCQGIPKANLQIPGPDFIDRVLLNSD
                     RKPGVLRNLQALYNHGRLAGTGYLSLLPVDQGVEHSGGASFAPNPLFFDPENIVRLAI
                     EGGCNGVASTLGVLGMVARRYAHKIPFILKINHNELLTYPNKFDQTLFASVDQAFEMG
                     ACAVGATVFFGSEESRRQIQEISEAFAHAHELGMVTILWAYTRNTAFKTPEKDYHVSA
                     DLTGQANHLAVTLEADIVKQKMAENNGGYTALKFGKTNAKVYSELTSDHPIDLVRYQV
                     ANCYMGRVGMINSGGESGKDDLHQAVRTAVINKRAGGMGLISGRKAFQKPFDEGVKLL
                     NAIQDVYLSQQITVA"
     gene            complement(16944..17792)
                     /gene="rpoH"
                     /locus_tag="PK522_02620"
     CDS             complement(16944..17792)
                     /gene="rpoH"
                     /locus_tag="PK522_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HNC40661.1"
                     /translation="MTDICALPSVVGGNIESYIQAVNRCPILSQEEETGLARSLRNDG
                     NIEAAHKLVLSHLRVVVAVARGYLGYGLPHADLIQEGNVGLMKAVKRFDPERGVRLIS
                     FAIHWIKAEIHEFIMRNWRLVKIATTKQQRKLFFNLRSMKQSLDTMSQAEVNTVAKQL
                     GVKPEEVVEMETRFSGRDVSLEPSGDDEDDITSPIHYLTDGSDPAQQVEQEQIARLYH
                     DKLQESLASLDDRSRHIIEARWLREQDTATLHELADELGVSAERIRQIEAKAIQKIKS
                     TVGAVL"
     gene            complement(18028..19269)
                     /gene="glcF"
                     /locus_tag="PK522_02625"
     CDS             complement(18028..19269)
                     /gene="glcF"
                     /locus_tag="PK522_02625"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HNC40662.1"
                     /translation="MQTNLADFVKKNQNSTEAEAILRSCVHCGFCLATCPTYQLLGDE
                     LDSPRGRIYLMKQVLEGQAVTQKTQLHLDRCLTCRACETTCPSGVRYSHLLDISRGIV
                     EEKVARNRYSELKRFAIRKFLTSRSIFKFSVRIAQLLRAFMPAKLKAAIPPLQRSAVN
                     WNNSRHPRKMLVLDGCVQPTLAPNINAATARVLDKLGISLIKVEEAGCCGALAYHLNA
                     QQQGLDAMRHNIDVWWPLIETHQIEAIVISASGCGVTVKEYGHFLQYDAAYAEKAQVV
                     AALAKDISEIITAELENLTVLLKETSLNHPGKLAFHSPCTLQHGMKIRGVVEKILSSA
                     GITLCQVDNPHLCCGSAGTYSILQPTLSQQLLKDKVIALSANGPAQIVTANIGCLMHI
                     QSGTTLPVKHWIELLDTLIHT"
     gene            complement(19273..20346)
                     /gene="glcE"
                     /locus_tag="PK522_02630"
     CDS             complement(19273..20346)
                     /gene="glcE"
                     /locus_tag="PK522_02630"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HNC40663.1"
                     /translation="MQNLIDHFIQSIQSAASARRTLLIQGSGSKHFYGNPAQQAEMDV
                     LDITPYSGIVDYDPSELVITAKAGTRLVELESLLLQSNQMLAFEPPHFTEHTTLGGTI
                     AAGLSGPRRAYQGSVSDFVLGIRMLDGQGNDLRFGGRVMKNVAGYDVSRLMVGSLGTL
                     GVILEASLKVLPRPETELSICLEMNQSQAIEKIHQWASKPLPISASCYHENKLLIRLS
                     GSPAGVSTACQKIGGELLESSDSFWRSLRNHTHPFFTSGKRLWRLSVKSTLLPIALEG
                     EQLIEWGGALRWLATDGQENDQTLRQQIGDLNGHATLFYGDKSNIQAFHPLSPSMLKI
                     HQKLKQQFDPLSIFNHGRMYSEF"
     gene            complement(20388..21842)
                     /locus_tag="PK522_02635"
     CDS             complement(20388..21842)
                     /locus_tag="PK522_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNC40664.1"
                     /translation="MSKQDLINQFRTFLSADSVLFESEDLKPYECDGLSAYRQVPLIV
                     VLPETEEQIISILQICHTTQTPVVARGAGTGLSGGALPIDNGVLLSLAKLKRILEVDP
                     VARTARVQPGVRNLSISQAAEPFGLYYAPDPSSQIACTIGGNVAENSGGVHCLKYGLT
                     VHNILKLRVLTIEGDILEIGSEALDSPGYNLLALMTGSEGMLGIVTEVIVKLIPRPEK
                     AQLVMAAFDDVQKAGDAVAQVISAGIIPAGMEMMDRITIHAVEDFLHAGYDLDAEAIL
                     LCEADGTHEEVEAEINKINTIMQQSGAIKISTSRDENERLRFWAGRKAAFPAAGRVSP
                     DYYCMDGTIPRKHLAQVLQGIESFSTEFGLRCMNVFHAGDGNLHPLILYDANQPGELE
                     KTEAFGARILEMCIELGGTITGEHGVGIEKIDQMCIQFKTKERALFHQVKNAFDPTGL
                     LNPGKAIPSLHRCAELGGMHVHQGKDKFPSLPRF"
     gene            complement(21845..>22463)
                     /locus_tag="PK522_02640"
     CDS             complement(21845..>22463)
                     /locus_tag="PK522_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNC40665.1"
                     /translation="PSRNWRICIITAIFAEKLSKYGCNYADAPVSGGDIGAQNATLTI
                     MVGASESVFNEIQPLLALMGSTITLVGDVGSGQICKIANQIIVALTIEAVAEALLFTS
                     KAGADPQKVRQALMGGFAASRVLEVHGNKMINRTFEPGFKIALQQKDLNIALSCASEL
                     GMSLPNTAVTQALYNACGAQGGNKWDNSALVRILEIMANHEIQQH"
BASE COUNT         6149 a         4489 c         4971 g         6854 t
ORIGIN      
        1 atctcttcct cctcctctca aatcatttta tctccggaaa acccggggcg attcatattg
       61 gttccacgac attcgtctga gatccgcaac tttattccca ttggctttct cgatgcattt
      121 tacattgcag cagatagttg tctttttatt cccaacgcca cgctctatca tttcggcatc
      181 ctcacttcca cgatgcacaa cgcctggatg cgcacggttt gtgggcgctt gaaaagcgat
      241 taccgctatt cagccgggat cgtctacaac aatttcccgt ggccggaacc cagcgatacc
      301 caacgcaaaa ccatcgaaac cgccgcacag gccgtgctcg atgcccgcgc gcaatttccc
      361 agttccacgc tggccgattt gtacgacccg ctagcgatgc cgccagaact ggtgcgcgcg
      421 catcagacac tcgaccgcgc cgtcgacgcc gcgtacggca aaaaatcgtt cgcttccgaa
      481 gccgagcgcg tagcgttttt gttcgagcgc taccaagcga ttatgagtac tttgccgatc
      541 gtgtcagcta aaaagggtag aaaaaaacgt ggtaaataat ttacaccatt ttttgatgat
      601 gttactcgtg ccgttatttg atttaatcag ttctattggt gaacttatat taaacccaaa
      661 gagagttgtt aacttattgg aaacctatca taaaatacaa aaaacaccct actgcaagca
      721 gtagggtgtt atggcttaat tatgtttagt ttaatgcggg gagtctcagg cgtaaactgc
      781 gtaaagagct gctcaaccat aatccaaata agctaatgcc aaacaaagcc agtgtgcctg
      841 gttcagggac ggtcactggt tcatcgccgc taatttggct aattttaaaa taatcatatt
      901 gatcacacgc actgcgatga cgagggccgc aattcgttct tgaatcaaat tcagtgggtg
      961 caatgattag ataacgtcca atcgtgtctg gatccagatc ttgagtggta ttcactgaaa
     1021 cgttgttaaa cgtgaattta gtgaatccca agccggctaa atctgaaaaa tgtttacccg
     1081 taaaatcgta gtttgcacca aggttattac cgccgatcca ggctgtgatg tccgcatcgt
     1141 tctgtttcca gccaatttgg aatcccgtaa aaagataact gggatcatag tgctgaaaga
     1201 cgacgaggtc atctttgcca tcattgtcgg tcgcatggtt tggcgagcca ctttcattcg
     1261 aatcaaatgg attttttacg ccaatcccac cagaccaaac ggttaacgtt gctttagcga
     1321 acgctccggt accattatta ttattggttg agtaagcgtt tactttgatt tcttcaccgg
     1381 tattgctggt gaattcacag gtattaccaa cccccgagtt gttacaatta tcggttgatg
     1441 tatttaaatt ccagtgcagt ggcgttgcgg ctaagtgtgt agtaaaacca atactgacta
     1501 ataatccgat cgttaaaatt ttgttcatct tgatcctttt aagaaaatat taaaaatgca
     1561 gaattcctct tgacttattt agagaaatta gtaaaagatt aatagcaatt catgtgccat
     1621 gaatataaat actttaataa acaataagtt atgcaactat ggttatttct gagggtatcg
     1681 atttgtaatg tttatcgaca gcgtaggttg atggggttat tactttattg aaggctattt
     1741 aattatttat aaaaataaaa aaactgcaag atgcgggtgg ttattgagtt tagtagattt
     1801 gactgaggga attttaggaa atagctagga aaacttgctt ttattcagca tttaaattga
     1861 attgattaca gtaaattagt gtccaatggt ttagatattg cgatcgtcag tgatgtacat
     1921 agcaatttct gttttgagtt tctccttgtt tgacaaattg tggcttgaat taggttgaag
     1981 tacgtgacga ggcccctcag accctaccca atgtggtagg gttttttttg tattattatt
     2041 ttactgatta ctgcgtccca tagcgctcac gataggcctg aatggcttgt aagttttgtc
     2101 gcatatttgt agcttctttt aaataatcga caatatcatt gagatcaata atgctgatta
     2161 ccgggatatg gtgcaagttg ttgacttcct gaacggcaga tagcttattt gaaccttgct
     2221 ctaaccgatc gagcgcaatg attacaccac aaggcgttgc tgattcagcc cggatgagat
     2281 tgacggattc tcctattgaa gtccctgcag aaataacatc atcagcaatg agtacgcgcc
     2341 cactgagtgg tgccccaatc gttatgcccc cttcaccatg atcttttagc tcttttcgat
     2401 taaagcagaa agggtaattg taaccaagct cagcaagcgc tatcgcgatg gtgcttacca
     2461 atggaatacc tttgtaagct ggaccaaata acatatcaaa gggaagttgg gcggcgataa
     2521 ttgctttagc gtagaattgg cctagttgac gcaaagcgtt accattattg aataagccgg
     2581 cattaaaaaa gtagggtgat aatcggcctg ccttggtttt gaattcacca aagcgaagga
     2641 cattacagcc aatggctagt tcgataaatg cttttttgta atctgacata gtttaattta
     2701 gtagcataaa tgagaattat aacgcttaac gtaaatggga ttcgtgctgc agccaaaaaa
     2761 gggcttttta attggttacc gttgcaacag gctgatgtaa tctgtgtgca ggagctgaaa
     2821 gcagggattg acgatatgag tcctgaaatg aaagcatttg acggctatca gggctatttt
     2881 cactgtgctg aaaagaaagg gtacagtggt gttggggttt atacacggaa caaaccagat
     2941 caggtgattg aaggcgttgg tattccggga atcgattcag aaggtcgcta tttgcgtgtc
     3001 gattttggct cgttaagcat tgtttcaatt tatcttcctt ctggctccag tggtgagcat
     3061 cgccaggatg ctaaatttta ttttatggaa cattttttcc cctttctgga ggaattttca
     3121 aaggagggaa gagaaattat tctgtgtggt gattggaaca ttgcccataa agaaatcgat
     3181 ttgaagaatt ggcgatccaa tcaaaaaaac tctggatttt taccggagga aagggcgtgg
     3241 ttatcagcgg tatttgaaga actgaaattt gttgatgtgt tccgtcgcct aaattcagaa
     3301 tccgagcagt atacctggtg gtcaaaccgg ggccaggcat ggactaataa tgtaggctgg
     3361 cgtatcgatt atcaaattgc aacagcttcg attgcggata aagcaacaca agtttccata
     3421 tataagcaag aacgtttttc agatcatgcg ccactgatca tcgattacga ctaccaatta
     3481 tgaaaaatac ttcgctaatc gcatggctag atgcatgccg gatttatctt catccgagag
     3541 tgatgggaat gttagcgctg ggtttttctg cgggactgcc tttattgctg attctaggca
     3601 cattatcatt ttggttaaga gaagcaggca ttgatcgaac cacgatcgga catttaagtt
     3661 ggattggctt ggcttacggc tttaaatggt tgtggtcccc cttggtcgat cgtatgcctt
     3721 tgcctatctt gactaagatg ctcggtcgtc gccgtgcatg gttgttattg tcacagatta
     3781 cgattgtgat agcactgatt ggtatggcaa gcacagatcc gctattgaac ctaacgcaca
     3841 tggttttttt tgcattagcg gttgcttttg cctccgcaac ccaggatatc gcacttgatg
     3901 cttatcggat cgaggcggtt gcaatagagc tacaaggtgc gatggcggca acttatcaag
     3961 cgggctatcg attggctatg atactttctt cagcgggtgt tctttggatt gcggcttcgc
     4021 tcgatacaat gcctacgggt tatgattatc gtgcttggca aatcgcctat tttgtaatgg
     4081 cggccttcat gggaattggt gtgctcacaa cactgattat ccaagagcca gcggtgaaaa
     4141 tcagtcagct tgctgttgaa aacgaaaaac gagtagaagc gatgattgct cgctggaatt
     4201 taaatcatcg acttgcccaa ttattactgt ggttctatgg tgcagtaatc gcgccctttc
     4261 gggattttat tgtgcgtcac cgctacaatg cattcttaat cttagcattg atcgcagttt
     4321 atcggatttc tgatgtggtc atgggggtga tgagcaatcc tttttacgtc gatatgggct
     4381 ataccaaaga cgaagtcgcg acagtttcaa aagtttatgg tgttctgatg acgattgcag
     4441 gcgcagcggt ggggggggtg ctcattatgc gaatcgggat tattaaaacg ctgtttattg
     4501 gtgcaatact atctgcagta accaatctat tgtttgtatg gttgacggta atcggccata
     4561 gtttacccgg attaattttt accatttcag ctgataatct ttcggcagga attgcttcat
     4621 cagcgtttat agcttatctc tcaggtctga cgcattctgc ttattctgcg acgcaatacg
     4681 cattgtttag ctcagtgatg ctacttttac ctaaatttat cgcaggattt agtgggcagt
     4741 ttgtcgatgc ctatggctat gcctggtttt tcctcagtac tgcactacta ggattaccgg
     4801 ttctgatctt ggtttggctt gttagtcgta tcgatttcca ttcatcggaa tagcccgagt
     4861 gtcgtaatgc ttggcctttt tctctctttg aattgagcta atctcatagg attggtaaac
     4921 aataggtaat ctaccagaat gactacttga tacgctaaag cctttgctga gtaatatgat
     4981 taataatccg gaagttaata atttttcata gattgccgtc atatgaatca ttatcgattt
     5041 ttattgttaa gtctatgcaa tccaaaacga tcactggatt aaaaaactgg attagttttt
     5101 ttaataaagc agaaatccca gtcttgaaac taacgggtaa tgagctgttt ttattgcttc
     5161 agcaggaaaa aaatcctagc gctcgcagtg ttgcaaacat cattaaacga gatccactga
     5221 tgactgtgaa attacttcgc tatttttgtc agcataagca caggagtctg gaacacgaag
     5281 tgatgcaagc tgagcagatc ttgattatgc ttggggttga taaggcgatg aagaatgttt
     5341 taccagagcc gacggttgaa gaattgttgc gcgagcagaa agttgaagtt cttattaatt
     5401 tacttcattt agttcatcgc tcccatattg cctcaaacta tgctttcgaa tgggctgttc
     5461 gtctccatga tacgcatttt gaagaaatac gtattgccgc attgctacat gatatttcag
     5521 aattattaat gtggtgtttt gcgcccacgg agatgctaag aattcgagaa atgcaaaaaa
     5581 aagataaaac actacgaagt tatcttgctc aaaaacaagt actcggattt acgctagccg
     5641 atttgcaatc agaattagtt ctgagatggt cattacccga gctgttgatt acgcttatgg
     5701 atgaccacaa tgcgtcacta caacgtgtgc gtaatgtcgt attggcagta aacctagcgc
     5761 gccattctgc aaatggatgg gatgatgcag cattaccgga tgattatact gcaattggtg
     5821 aattattaag gatgccagta catgatgtga agataattgt cggcgcaaaa aatattgagc
     5881 cggtcaattc tcagcagtct gaacattagc taataattat tagattattc gatattctgg
     5941 acttgctcac gcatctgctc aatcagtacc ttaagttcaa ctgaaatttt tgatacttca
     6001 acatcgagtg atttcgaacc caatgtattc gattctcgat ttaactcttg cattaagaaa
     6061 tcgagacgtt ttccacttgc cccaccttta agtagggttt gctcgacttc atcgagatgc
     6121 gtattgagtc gtgataattc ttcgtcaata tcgattttac ttgcaaataa aacaagttct
     6181 tgtcggatac gatcgtcatt gcaatcaatc tgtgcctctt gcaagcgtaa gattaatttt
     6241 tcctgaaaat tcttaatgag tgttggaata cgtggtgata acgctgaagt taactgacgc
     6301 atttgggtta gtcgatcaat caaaatcgct ttaagtttct caccttcacg taatcgtgtt
     6361 gagacgagtt ccgtcaacgt tatttgtagt aagttgttac acgcttcttg taaatcagtg
     6421 gttggcgaag aatcactcac taagatgcca ggccatttaa gaatttctgc aacactcaag
     6481 cttcgcgcag caataaaatt tgattgaact gtattattta gatccagtaa ttgacggatt
     6541 acttgggaat taagctgttg gggaaggttt aaatcgggat aggattgaaa ggttaggcgg
     6601 caatcaattt ttcctcgttt tatttttgta ttcagcaatt cacgcatacc cgcttcgaag
     6661 ctacggaatt catcgggtag tttgaattgg atatcaagat aacgattatt aacagaacga
     6721 agttccatgg aaatcaggcc actggaaatt tcctgggtcg ctgtggcgta tcctgtcatg
     6781 ctagtgatca taatgcgatt atcctgttga catttaataa tgtgtaagct tacccctgtt
     6841 ttctctactc atggacttgt tcccataatt tttgtagacg cttaactgaa acagggtaag
     6901 gtgtttttaa ttcttgagca aataatgaaa tcctcaattc ttcgatttgc cagcgaaatt
     6961 cttcaagctt tggatctttg aatccacgct tgctgtgttt ctcatatctt tggcgatatt
     7021 gattccataa tactgagatg gcaagcgcat gctggttatc acgtgctgga ttgttagggt
     7081 acttatctaa ccgaacgagc attcctttta agtaccgaag taagtgtgga agtcttaccc
     7141 agtcagtttg ctgcaggaaa ttaggataaa tgagcgcctg aagctgattg ttcaattctt
     7201 gttttatttg agcatgatgc ggattgacag tggacagttt ctgtttgaat gcctgatatt
     7261 cgagcgcaag atcatggata aattgaataa gtgtgtcagc gacggagttt aaacgaattc
     7321 ggcctttctg cttcaactgg gaaaactcat gttcattccg tggtaaaggt tcatcgccaa
     7381 tcaaggcacg atcatagatt gcggcaaaca tatcttgctt gagtgtttct gaatcgatga
     7441 gatggcttaa tactaacgaa tcagacttgg tgcttggtaa tttcttttct aatagcttaa
     7501 tagggtcttt taattccaaa atgagcagtc gtttgacgcc acttcgcata tgattgtgcg
     7561 ctacctcgat agtatcaaat aagcgaattg caacatggtc aatctgatca acgagcgctg
     7621 ggtatcctgt taattgtttt tgtgctcgaa taaaagtgac ttcctcaggg agttggccaa
     7681 agttccattg cgtaatttga tcacgttcta taccaagctg atttgctgcg ttattttgcg
     7741 aaaagatcag ttgagcagtt tgcccaaatt gatcttgtaa ttctattaaa tcacggttta
     7801 ccccaagctc tttcccggca tcatcgataa ttcgataatt catttgcagg tgtaatggca
     7861 ttaattttgt atcccaagta tcggatgaga tttttgtacc tgttatgcta aaaatatact
     7921 cagaaagcgc ttcaagtaag gaaagcgaat gcttttgctt atcacggtaa tctaggaaat
     7981 gggttacatg ctcagcaata ggaatgagat tgcgtcgaat tggcttaggt aatgctttaa
     8041 agcaccaggt aattttttca cgaattaaac cgggtactaa atcttgaaac tgattcgctt
     8101 ttagtttatt taatgaatga agtggggcag ttactgtaac gccatccata gcgtgacccg
     8161 gctcaaaacg ataggtgagt gggagttgtt gatcaaaaat ggagattgat tcaggaaata
     8221 gcgtttcaaa agctgtatct gctttatggc gcattaaata ttcccggcta agataaagca
     8281 aatgggggtt atcctgctct acttgtttgc gccagctctc aaatgagctg ccattcaata
     8341 tattgtcggg aattattttg tcgtaaaaag taaaaatatc ttgttcttcg actaaaacat
     8401 cttgacggcg ggttttgtgt tcgagttctt cgatgtcttg aatgagctgg cgattatgtt
     8461 taaggaattg agcattggtt tcgtagtcgc caagaaccaa tgcattgcga ataaaaattt
     8521 cccgagcttc tttggggttc atcttcccat acgtaacgct gcgcttcggc acaatcataa
     8581 gtccatagag cgtaacgcgt tcatatacca taacttgtgc agttttcttt tcccaatggg
     8641 gatcaaaaaa atgtttttta caaagcttgc ctgcaatttt ttcaagccat gtcacatcaa
     8701 tcgtagcagc acatcgtgca tagagctttg tcgtttcaac gagctcagct gctacaatcc
     8761 acttggcttt tgcttttctt agcgtagagc caggataaat aaagaatttt atccctctag
     8821 cgccaagata ctccccttca ttctcacttt taaacccaat attgctaagt aaaccggcta
     8881 gtaaggcacg atgaatttca tcataacttg ctggaacttg gttgggcctt agtccaaatt
     8941 ccagaactaa cgtatggagt tggccgtgaa tttcacgcca ttcccgcatt ttgcgatacg
     9001 aaataaagtg ctcgtgacat tgtgtgatta attttttctt tgattttttg tgtttaagca
     9061 attcatcaaa gaaattccaa agtttcagaa atccaataaa atcagagcgc tcgtcatgaa
     9121 aggggagatg agcacgatct gctgcttctt gttgatcaaa gggtcgttca cgcggatctg
     9181 ccaaacttaa taccgatgca atgatcaata cttcgcttaa gcagttttct tgtttggcgg
     9241 caagaatcat gcgtgcaatt ttgggatcaa ttggaaattt tgcaagttgc cagccaatgg
     9301 tcgtcagttt ttgatgaata tctagggcgc ctaattcgct gagcgattga tagccatccg
     9361 caatcatgcg aggtaaaggc ggctgaatga atggaaagtt ctcgatatca ccaatcttga
     9421 gggatttcat gcgcaggatg accgaagcca atgatgtgcg gagaatctct gggtcggtgt
     9481 atagtggacg tgtttcaagc tcttctttag cgtagagtcg aatacaaata ccactcatga
     9541 cacggccgca tcgtcccgca cgttgattcg ctgaagcttg tgaaattttt tcaacgagta
     9601 actgctctat tttattgcga tggttatagc gattaattcg tgcaacacca gtatcaatga
     9661 cgtagcgaat gcctggaacg gttaacgaag tttcagcgac attggttgca aggatgatgc
     9721 gactgactgc gtttgattta tgaaatacac gttgctgctc agcataagat aatctggcat
     9781 agagggatag aatttctagt ttcccaggat cactgaaatg attaaaacga tgcttacgaa
     9841 gggtctcgga cgtttcctta atttcacgtt caccgggtaa gaaaattaat atatccccta
     9901 ttccagtgcg tagaagctca tccgtagcgt ccaaaatgga ttgctgcaga tcgtattctt
     9961 cctcatcttg attattatct tttgcgtggt agcgtatttc gactgggtag gttctgcctg
    10021 agacttcgat tacgggcgca ttgttaaaat ggtgcgagaa gcgctcagca tcaatggtgg
    10081 ccgaagtgat aatcagcttt aagtctgctc gtttcggtaa taattgcttg agataaccta
    10141 ataaaaaatc aatgttaagg ctgcgctcat gagcctcatc gatgataatc gtgtcgtatt
    10201 tttgcagata agggtcgctt tgcgtttcag caagtaaaat accatcagtc atgagcttga
    10261 tataggtgtc ttgattgatg ttatctgaga aacgaacttt gtaacccaca gcgtgatcga
    10321 gcgggctact cagttctgtt gcgatttgta ttgcgacaga tcgggcggca atgcgacgag
    10381 gctgtgtatg cccgattaac ccagagactc ctctaccaag ctctaggcaa aatttaggaa
    10441 gctgcgttgt tttgcctgat cccgtttcac cactaacaat aatgactgag ttagatcgaa
    10501 tcgcgtccag gatcttgtgg cgctgcttgc agataggtaa ttcatctgaa tacttgggtt
    10561 tgggtaaatt gttactcctt cgtgccagat tttcagcaga taggtgctgg gaaatactca
    10621 ttcgggctaa cgaacactta ccgatttaaa ggttgcaggg ggcgaaggca attatttgct
    10681 ataaaagatc gcaatgaaat tatttttcga caataacggt ctctttctga ttactcaagg
    10741 ttccctgttc aatgatggct acggttaatc gagagatcgc gacacgtttg cctgtggttt
    10801 cttcggtgat atcggtttgc catacttggg tgcgtcgacc aacatgaagg ggggtgcaga
    10861 taccaatcac atatcctttt gttacagcac ggatatgatt ggcattgatt tctaagccga
    10921 ctgcgcgata tttttctggg tcaatcgtca tccatgccga aacgctgcct aatgtttcag
    10981 acaagacaca gcttgcgcca ccgtgtaaga ttccaaaagg ttgtgtggtt cgttgatcaa
    11041 caggcatacg cgctttcaga aaattaggcc caatttcaga aaaatgaatg ccgatatgaa
    11101 tgcccatgtt ggcattgcgt agcccttcca agtaatcaag ggtatagggt ttaaaccaaa
    11161 tattatccat tgttatgtgt gcttctgaag agaaatgtaa acactttcca ctgtcaatag
    11221 cgattaggaa agcagctatt ttaattttat ttaggaagcg ctagtaacgt tagggtaatc
    11281 gatcttagta atcattaacg attccttcgc tgcggtcttc tgcgatgctg tttttattat
    11341 aatcgagcgg ttgcgtcaat gttgggcgat ttgatcgtat tcggtcaggc gctgggggcg
    11401 aagatagtag ttcatttcta gtgttaccga tgggggtagg cgtcgttaca tttgattggt
    11461 ggggttggtt gaattcattt ttttcattgg cttcttttgt tgttggaatg agcaaatttc
    11521 ccgttaagaa aacaaaagcg agggccagta aaattttctg acttttaaga ataaattgtt
    11581 cagtactgcg gttaattgag ctcgtcgtat taattttcca gtattttaat aatatagaaa
    11641 gaaggattgc tacaagaccc gcaaataacc aggtattatc aaaatattcc caggccgcta
    11701 aatcattcat tgttcaaaag ctagaattaa tctggttctt tggcttatta ctcatgatca
    11761 aggaaaatca atacgttgtt gcctatctta atgagccgcg cgcatttcga attgcatcag
    11821 ctaattgatg cacggacatt gcataaaacg agctgcgatt atagcgcgtg atgacataaa
    11881 aattcttaaa acctagccaa tattgaaagt tttcgttatc tttcagttcg atgagtgcag
    11941 cgagccgctc attctcgaac gattgtaatg ggataatact cgcttcttga agttgcttga
    12001 tggtatgttt gggttcgatt ccgctctcaa gtatttcctg taagcgatca gcttttacac
    12061 tggattgaac gacaattggt cctcccattt cccatccata ttcttttaaa tagttagcaa
    12121 cgctaccaat tgcatcggct gcactatttg ctagatcaac tttaccatca ccgtcaaagt
    12181 caacagcaaa tcgtctataa cttcctggca taaattgagg aataccaatt gcaccggcgt
    12241 aagaaccttt gatgcccagt aaatcaaaat tttgttcttg agcaagtaat aaatattgaa
    12301 cgagttcact ccgaaaaaac tcagctcttt taggaaaatc aaatgcaagt gtactgagtg
    12361 catcgatgac gcggtgtttt ccagttgatg caccatagga tgtttctaca ccgatgatgg
    12421 caacgattat ttcttcaggg ataccgtatt ctttgtttgc gcgctggagt acattggcgt
    12481 gctgattcca gaaactaact ccatttttaa ttcgttgcag attaacaaag cgcgaacgat
    12541 attgactcca aggaactgag gatgagggag tcgaaatcag attgataatt gaaggctgga
    12601 attgaacttt gctaaatatg gcttccagtt tgggcctatt aaatccatgc tcagcgacca
    12661 tttcatcgat gaaagtctta atttcaggac gagtgggaga ccctattgcg gtagctcccc
    12721 agagcagcat acaaacccca agtaccaaaa taaccatgta gtaggttggg agcctgtgaa
    12781 tgtctttttt taatttattt ttaggtattg gtagcctcat aatagactga tataaagttg
    12841 ggaagcttga tttaatattg attatttagg ctgtcttaat ccaaaataga gtctactcgt
    12901 aaatatattg ggatgtcatc gaatattttt ccgaatgata tgcattgctt gcttcccatt
    12961 ttcttttttt ttatatacta tatccattat actgattaca catcccaatt taatcccatt
    13021 ccagcttaaa aagtcttaat gacaatataa ctaaacttaa tttcgaaatt aattaagtta
    13081 atggttatca atatctgaaa tcgatacata cgaggtattc atggctcaac atattcatta
    13141 tataactgat gcaacatttg aagcggaggt tttgcaatca cccaccccgg tgctagtcga
    13201 ttattgggct gagtggtgtg gtccttgtaa aatgattgca cctattcttg acgaggtggc
    13261 caacgaatat aatgaccgcc ttaaagtcgc taagctaaat atcgatgaga atcaacacat
    13321 cccccagaaa tatgggattc gtggaattcc aacgttaatg ttatttaaaa acggcaatat
    13381 tgaagcgact aaagtgggcg ctttatcgaa gtctcagcta actgccttta ttgatagcca
    13441 cctttgagat aactaatcaa gctatttgac aacaaattaa agaatcaatt tgtattcctt
    13501 tagtgatcag tcagcaggtt acaacatcta agtctcttca agtttttctt ttctgatctt
    13561 tcaatcatat tctccatgcg cttatctgat ctaaaaagcc ttcatgtttc cgaattaatt
    13621 aaaatggcag ttgcaaatga agtcgaagga gccaatcgcc tgcggaagca agatttaatt
    13681 tttgccttat taaaaaacca agcacgtaag aatgagagta tttttggtga aggtactttg
    13741 gaaattttgc aagacggttt tggcttcttg cgctctcctg atacttcgta cttagcaggt
    13801 cctgatgata tttatatttc tccaagtcaa attcgacgtt tcaacctgca tactggggat
    13861 tcggtagatg gagagatacg tcctcctaag gatggtgagc gttattttgc tttagtgaaa
    13921 gttgataaag tcaataatga gccgcctgaa aattctaaac gcaagattct gtttgagaat
    13981 ttaactcctt tattccccac tgagcgttta gtactagaac gcgatatccg ggccgaggag
    14041 aatacgactg gaagaataat cgaccttatt gcaccgatcg gtaaaggaca aagagggtta
    14101 ttggttgcta gtccaaaatc tggaaagaca gtgatgctgc aacatattgc acactcgatt
    14161 gcagccaatc accctgatgt tattttgatg gttttgctga tagatgagcg ccctgaagaa
    14221 gtgaccgaaa tgattcgttc agtaagaggt gaagttatct cttccacttt tgatgagtct
    14281 gctgccaggc acgtacaggt tgcagatatg gtaatagaga aggccaaacg actagttgaa
    14341 cataagaaag atgtcgtgat attgcttgat tcgattactc gtttggcgcg tgcttacaat
    14401 acagtcgtcc ctgcttcagg taaggtactt actggtgggg ttgatgctaa tgctttgcag
    14461 cgccctaagc gattttttgg tgccgctcgg aatattgaag aaggtgggtc attgaccatt
    14521 atcgccactg cacttgtgga tacaggatct cgtatggatg atgtgatata cgaggaattc
    14581 aaaggtacgg gtaatatgga gattcattta gatcgccgaa tggctgagaa gcgtatttat
    14641 ccagccatta atgtcaatcg ttctggaaca cgaagagaag aattattgct gccacaagag
    14701 atacttcaaa aaatatggat cttgcgcaaa ctgctttatc caatggatga tatggatgca
    14761 atgtcattct tgttggataa aattaaagca actaaaaata atgctgactt ttttgattca
    14821 atgcgcagag tataattgaa ttaggcaggg ttaatattta gtactatttt cttacccccc
    14881 cactagcaaa tctctgattg tatattgagg ggattaccgc aagatgtgcc aaggcgcgat
    14941 gtttttttcg ctgagcaagc ggtgtttgct aatgccagtt tcgtcaaatt cggcttcctc
    15001 gtacgcattg atctggaaaa catttcctga agggctatgg atcttgatgt gcccgtcggt
    15061 aactgcaatg tggcccgtgc cgtccttatg taattgaatg ctaaaacggg tgccaaaatc
    15121 tttgatgatg gtgctaccta cttttacttc caattgatgg gtggcctgtt tctgaatatc
    15181 aaaataaaca tttcctttaa atagctcgac gtgtaacggc tcacttagtc tcacagcaac
    15241 cgagctctgc atatctagcg ccatattgat acccggtgct agtggcgccg ttaaaggggc
    15301 ttccgatttc gtttcgtaaa agcggatact cggcggtttg cccaagtacc aagtcatcaa
    15361 tcctagaaat acacaaaagc ccatgatcgc actatgcacg aaaaagcgct tccagctaaa
    15421 aacgaaagta taaggttcaa cgaccggggg tttggggtcc tgcattgctt cggtaaaaaa
    15481 tgaaaagaag cattatatac cgatgacgat cattgctttc ctaaatgtga tggatgagct
    15541 gagtgattgt tgattggctg gctgtaggcc atgaagctca gcagtggggc cgcaacgcaa
    15601 ctgcggatgg agtaagctat agcttaaagg ggaagcaatt tgtgagtggg aggagcgcta
    15661 tattcaagaa cacgaaacat cgctgggtaa ggcgcgatta actagtggtg gaatacggca
    15721 aagatggtgt ttgtcgtcgt ggttttatcg atgttttgag ttgctttgtt acctgatgct
    15781 atttttgggc gtatactcaa aaaaatggtg ataaggagta agcaatgact gatattgtta
    15841 atttattggg tcaagaagca gatcaattat tagcgcacgt ttgccaaggc attcctaaag
    15901 caaatctaca aatacctggg cctgatttca tcgatcgggt gctactgaat agcgatcgta
    15961 aacctggcgt attgcgtaac ctgcaggcac tttataacca tggtcgcttg gcgggaaccg
    16021 gctatctttc attactgcca gtggatcaag gagttgaaca ttccggcggt gcttcgtttg
    16081 caccgaatcc attgtttttc gaccctgaaa atattgttcg acttgcgatc gaaggggggt
    16141 gtaatggagt ggcatcgact ttaggtgtat tgggtatggt tgcgcggcgt tatgcgcata
    16201 agattccgtt cattctgaag atcaatcata acgagttatt aacttacccg aataaattcg
    16261 atcagacgtt atttgcgagt gttgatcagg cttttgagat gggcgcttgc gcggtgggcg
    16321 ccacggtttt ttttggctct gaggaatcgc ggcgccaaat acaggaaatt tcagaagcct
    16381 ttgcgcatgc gcatgagcta ggaatggtga ctattctttg ggcgtataca cgcaataccg
    16441 ccttcaagac acctgaaaaa gattatcacg tgagcgcgga tttgacgggc caagcgaacc
    16501 atttagcggt gacgctcgaa gcagatatcg tcaagcaaaa aatggctgag aataatgggg
    16561 gctacacagc gcttaaattt ggtaaaacca atgccaaggt ttatagcgaa ttaactagcg
    16621 atcatccgat tgatttggtt cgatatcaag tagccaattg ttatatgggc cgagttggca
    16681 tgattaattc ggggggtgaa tccggcaaag acgatttgca tcaagcggtg cggacagcgg
    16741 tcatcaataa gcgagctggc gggatggggc tgatttctgg tcggaaggct tttcaaaagc
    16801 catttgatga aggcgttaaa ttactcaatg ccattcagga tgtttacctc agtcaacaaa
    16861 ttactgtggc ttagcgctgc attcgcttaa ttattgagtt agctgtgctt cttgacctga
    16921 agcacagcca ttaaggtaga ttgttataga accgccccaa cggtactttt gattttttga
    16981 attgcttttg cttcaatttg gcgaatgcgc tctgctgata cgcctaattc atctgcaagt
    17041 tcatgcagcg ttgcagtatc ttgctctctt aaccagcgcg cttcgataat atggcggcta
    17101 cgatcatcta aactggcaag cgattcttgt aacttatcat gatataagcg ggcgatttgc
    17161 tcttgttcga cttgttgagc gggatcggaa ccatccgtta agtaatgaat tgggctagtg
    17221 atatcatctt catcgtcacc actcggttct agtgaaacat ctcgaccgct aaagcgagtt
    17281 tccatttcga ccacttcttc cggtttgaca cctaattgtt tagctaccgt gtttacttca
    17341 gcttgcgaca tcgtatccag gctttgtttc atactcctga gattgaagaa aagtttacgc
    17401 tgttgcttcg ttgtggcgat tttaaccaac cgccaattac gcatgatgaa ctcatggatt
    17461 tcagctttta tccagtgaat cgcaaatgaa attaatcgta ccccgcgttc gggatcaaag
    17521 cgttttaccg ctttcatcaa gccgacattt ccttcttgga taagatcagc atggggtaat
    17581 ccatagccta aataaccacg ggctaccgca actacaaccc gtaaatgcga taggacgagt
    17641 ttatgtgcag cttcaatatt gccatcattt cttagtgagc gagctaggcc tgtttcttct
    17701 tcttgtgaca gaatggggca acgattaact gcttgaatat aactttcgat attaccaccg
    17761 acgacagaag gtagcgcaca gatatcagtc atattattcc tatctccttt actgttggga
    17821 tgattatttg caaaagattt atcttgattt tagcactcgc tgcataagag tgctaataat
    17881 ttttaaaggt tcccaaactg aattgatttc gttgagaata aatcgattgc ttcttgttca
    17941 ataaagaaaa aagccatatt ttctgccata tttcagtcaa aacaactcac tgttggttca
    18001 acttatttaa cgggggaggg gcggtgttta agtgtgtatc agtgtatcca gtaattcaat
    18061 ccaatgtttg acgggtaggg tggttccact ctgaatgtgc atcaagcagc caatatttgc
    18121 agtaacgatt tgcgctggtc catttgcaga taatgcaatt accttatctt ttaggagttg
    18181 ttgagatagt gttggctgca aaattgagta tgtacccgct gatccacaac aaagatgagg
    18241 gttatcaacc tgacacaagg tgataccggc tgaactgagg attttctcaa cgacacctcg
    18301 aattttcatt ccatgttgca gtgtacaggg tgaatgaaag gcaagcttgc cgggatgatt
    18361 tagcgatgtt tctttcaata gtaccgttaa attttccaat tcagccgtaa tgatttcact
    18421 aatatcttta gcaagtgcag ctactacctg ggctttttca gcataagctg catcatattg
    18481 cagaaagtgt ccatattctt tgacggtgac gccgcaacca ctggctgaaa ttacgattgc
    18541 ttcgatttga tgtgtttcga tcaaaggcca ccaaacatcg atgttatggc gcatcgcatc
    18601 taatccttgt tgttgggcat tgagatgata ggctaacgcg ccacagcacc cggcttcttc
    18661 taccttgatc aaagaaatac ctagtttatc gagaacgcgt gctgtagctg cattaatatt
    18721 gggagccagt gtgggttgaa cacaaccatc cagtacaagc atttttcttg ggtgacgaga
    18781 attgttccaa tttacagccg aacgctgcaa cgggggtata gctgctttaa gtttagcagg
    18841 cataaaagcg cgtaataact gcgctatcct aaccgaaaac ttaaatatcg atcgactggt
    18901 tagaaattta cggattgcaa agcgcttcag ctcgctatag cgattacgtg ccaccttttc
    18961 ttctacgata ccgcggctta tatctaatag gtggctataa cgaacgccgg aagggcaggt
    19021 tgtttcgcag gcccgacacg tgagacaacg atccaagtgt aactgcgttt tttgcgtaac
    19081 cgcttgacct tccaacactt gcttcattaa ataaattcga ccccgggggc tatccagctc
    19141 atcgcctaat aactggtagg ttgggcaagt cgcaagacaa aatccgcaat gtacgcagga
    19201 tcgaagaatg gcttctgctt cagtactatt ttgatttttt tttacaaaat cagcgaggtt
    19261 ggtttgcatt gtttaaaact cggagtacat ccgtccgtga ttaaagatac tgagcggatc
    19321 aaattgttgt tttaattttt gatgaatttt tagcatagac ggggaaagtg gatgaaaggc
    19381 ttgaatgttt gatttatcgc cataaaataa cgttgcatgc ccatttagat cgccaatttg
    19441 ttggcgaagg gtttgatcgt tttcttgccc atcggttgct aaccagcgta aagccccccc
    19501 ccattcaatg agttgctcgc cttccaatgc gataggtagt agagtagatt tgacggatag
    19561 tcgccagagt cttttgccag aagtaaaaaa agggtgggta tggttacgca gcgatcgcca
    19621 gaatgaatca ctcgattcga gcagttctcc gcctattttt tggcaagcag tactaacccc
    19681 agcgggcgaa cccgataacc taatgagtaa tttgttttca tgatagcaac tggctgaaat
    19741 cggtagtggc ttacttgccc actgatgtat tttttcaatg gcttgtgatt gattcatctc
    19801 caaacaaata cttaattcag tttccggtct tgggagtact tttaatgaag cttctaaaat
    19861 gacccccaag gttcctaatg agccaaccat taatcgagaa acatcatacc ctgcaacatt
    19921 cttcatgact cgaccgccaa atcgtaaatc attaccctga ccatcgagca tgcgtatgcc
    19981 tagtacaaaa tcgcttaccg agccctgata ggcgcgtcta ggtcctgaca atcctgctgc
    20041 aatggttccg cctaatgtag tatgctcagt gaagtgggga ggctcaaaag caagcatttg
    20101 atttgattgc agcaacaatg attctagttc aaccagtcga gtacccgctt tggcggtgat
    20161 aactaattca ctcggatcat aatcaacgat tcctgagtag ggagtaatat caagcacatc
    20221 catctctgct tgctgagcgg gattaccata aaaatgctta ctaccacttc cttggatgag
    20281 caaagttctt cgggcacttg ctgcagactg aatactttgt ataaagtggt caattaaatt
    20341 ttgcatgagt ttgcttgaaa gacttgagtt tgaagcctag ttatttacta aaatcgaggc
    20401 aatgaaggaa atttatcttt tccttgatga acatgcattc cacccaattc agcgcagcga
    20461 tgtaaagaag ggatagcttt gcctggattc agtaagccag taggatcaaa cgcatttttg
    20521 acctggtgaa aaagcgcacg ttccttagtc ttaaattgta tacacatctg gtcaattttt
    20581 tcgataccta caccatgttc gccggtaata gtgcctccca attcgatgca catttcaaga
    20641 atacgtgcac cgaacgcttc cgttttttca agctcaccgg gttgatttgc atcatataaa
    20701 atcaatggat ggagattgcc gtctcccgca tgaaagacat tcatacagcg taagccaaat
    20761 tcagttgaaa atgattcgat accttgcaat acctgagcta gatgcttacg tggaatcgta
    20821 ccatccatac agtaataatc aggcgataca cgaccggcag caggaaatgc cgctttacgg
    20881 cctgcccaga aacgaaggcg ctcgttttca tcgcgcgaag tgctgatttt aattgcacca
    20941 ctttgctgca tgatagtatt gattttattg atttctgctt cgacttcttc gtgggttcca
    21001 tcggcttcgc agagtaatat tgcttcagca tcaaggtcat agcctgcatg caaaaaatct
    21061 tctactgcgt ggatagtaat tctatccatc atttccattc cggcaggaat aatcccagca
    21121 ctaatgactt gtgctactgc atcacctgct ttttgcacat cgtcgaacgc agccatgact
    21181 agctgagctt tctcgggcct aggaattaat ttaacgatta cttcagtgac aatgccgagc
    21241 ataccttcgc tacctgtcat caaagcgagt aggttataac cagggctatc caaagcttca
    21301 ctaccgattt caaggatatc accttcaatc gttagcactc tcaatttaag gatattatgg
    21361 acagtcaatc catattttag gcaatgtact ccgcccgaat tttctgcaac attaccgcca
    21421 atcgtgcaag caatttgaga tgaaggatca ggcgcataat acagcccaaa tggttcggct
    21481 gcttgactaa tcgagagatt acgcacaccc ggttgaactc gtgcagttcg tgcaactgga
    21541 tcaacttcaa ggatgcgttt aagctttgct agggatagta aaacaccatt atcgatcggt
    21601 aatgcacctc cggataggcc agttcctgca ccgcgcgcaa caacgggcgt ctgtgtcgta
    21661 tggcaaattt gtagaatgct gataatttgt tcttcagttt ccggtaatac aacgatcagg
    21721 ggcacttgcc gataggcaga taaaccatca cactcgtatg gctttaaatc ttcagattca
    21781 aacaatactg aatctgcaga aaggaaggta cgaaattggt taattaggtc ttgtttagac
    21841 atcgttaatg ctgttgaatc tcatgattag ccattatctc aagaatacga acaagtgctg
    21901 aattatccca tttatttcct ccttgagcac cgcatgcgtt atacaaagcc tgagtaactg
    21961 ccgtatttgg taaactcatt ccaagttctg aagcgcatga tagcgcgatg ttcagatctt
    22021 tttgttgtaa cgctattttg aagcctggtt caaatgtccg attaatcatt ttattgccat
    22081 gcacttcaag cacacgagat gctgcaaatc cgcccattaa tgcttgcctg actttctgtg
    22141 ggtcagcacc agctttcgat gtaaataata atgcttctgc tactgcttcg atagtcaacg
    22201 caacgatgat ttggtttgct attttacaga tctgtcctga acctacatct ccaactaagg
    22261 taattgtgct acccattagc gctaatagtg gttgtatttc attaaacaca gattctgaag
    22321 cacctaccat gatggtaagc gtagcgtttt gtgcgccgat atcccctccc gaaactggcg
    22381 catctgcata attacagcca tatttactga gtttctcagc aaatatggct gtaattatgc
    22441 agatgcgcca gtttcgggag ggg
//
LOCUS       DAOQLJ010000019        22359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_797_length_22359_cov_7.030084, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000019 DAOQLJ010000000
VERSION     DAOQLJ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 22359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22359
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_797_length_22359_cov_7.030084"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="PK522_02645"
     CDS             <1..1255
                     /locus_tag="PK522_02645"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNC40666.1"
                     /translation="AGQWPESLKPGFEPARLQWLIDQEQSQQRLQWWVLQTSAQGYFV
                     CQPGSPETTSPQLPSAVLESLSAIPPTYQVLSGSTLSRRGIVRPAQVFSAADAPLVVE
                     TSMQRVELEAIQKPAWALRIKANRSGLAVDLPVWQGKSVTAPWQPGTGSSAGRWQLPV
                     PFGLDEWGLYADLTVREVTQRFRWIVPGTFTMGSPENEAERREDETQHLVTLTQGFWL
                     ADSVCTQALWQAVMGGDNPAHFRDSPNHPVEQVSWNDVQQFIQTLNAQFPDIQARLPT
                     EAEWEYACRAGTTTPFSFGGNITSEQVNYHGEYPYAGAEKGRYREKTVPVKSLPPNPW
                     GLYEMHGNVLEWCGDWYGTYPAEAVVDPIGPVTGQGRVLRGGAWGGPDLRSSLFGFRL
                     ALGHKPASKAGKAGETGRQGTDREA"
     gene            1255..2157
                     /locus_tag="PK522_02650"
     CDS             1255..2157
                     /locus_tag="PK522_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNC40667.1"
                     /translation="MNPQLLPPAFPTSWANEWGEDEFGLYLDCNVNGIVQRFRWIVPG
                     TFTMGSPENEAEREEDETQHFVTLTQGFWLADSTCTQALWLAVMGNNPTFPIIIGENL
                     FDNLSDKVKATQQQLFSNDDLDPSLVHPVTRVSWDDVQKFIQTINAQLPDLQARLPTE
                     AEWEYACRAGTITPFSFGNNITPEQVNYHGEYSYAGAEKGLHRGRTVPVKSLPPNPWG
                     LYEMHGNVWEWCADRYTAYPSFAVIDQSGSATKEYRSLRGGSWASHAGRVRSAFRDGD
                     EANFNDGVIGFRLALGRKASTSQV"
     gene            2349..3191
                     /locus_tag="PK522_02655"
     CDS             2349..3191
                     /locus_tag="PK522_02655"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNC40668.1"
                     /translation="MSGIFISYRRHDSQSAAGRLADFLERSFGADQIFRDVETIEPGV
                     DFVQAIEKAIASCQVMLVVIGPRWLTITDQQGNRRLDKADDWIRIETKLALQRNIRVI
                     PVLVENAQPPTMDALPDEIKSLARREAHELSDKRWDYDVQCLIEALEKIPGIRRKVQT
                     TTFSQNAPVQPVTTRLTTKLGKVLAWIGGIFVALIMIGVFLDDTTTSITPPLSPPLTP
                     VTPQPLMQGLPSGSPTSTCGCWGYVEVGATRPNPQCASGIDTVVMCQGVCAGGGAPWQ
                     SLCQ"
     gene            3361..4221
                     /locus_tag="PK522_02660"
     CDS             3361..4221
                     /locus_tag="PK522_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNC40669.1"
                     /translation="MDKSALLSALAAEIEQGKMIFPTSTSAAINIKKMLDDPDCELDK
                     VTRLVQAEPLLATKVVAVANSVVFNRSGKRVTDVRSAISLLGFNMVRNLATAILTRQL
                     AGKAANSEVVTQLWKHSAYVASLAQVIARRVTKQNPDTAMFAGMIHEISWFYLLSREK
                     DFPGLTDGNFASSWVSDEDLDDETEIECEIKIGTAILKALAVPAPVVEGITCLWEGYL
                     AYPPTTLGDTLLFADQLSPIRSPFVLPSDETGEDIRAKMDTLTDQETLSEILRDSEEE
                     VKSLTEALGT"
     gene            complement(4286..4717)
                     /locus_tag="PK522_02665"
     CDS             complement(4286..4717)
                     /locus_tag="PK522_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40670.1"
                     /translation="MSFISVLLNAFLSYVYLAYYPAVLQFVFEPDAAMIITGIVLFVL
                     LLMLARRSSIRNSFGTNYRLRPGITGSLIGCFGLLACYVAVNIMMEQPLPGSPSGQLL
                     ATIVDQIGPWITAAVIAGIGLQFVLFGLRLLRDTFHSKIYH"
     gene            5032..6102
                     /gene="aroG"
                     /locus_tag="PK522_02670"
     CDS             5032..6102
                     /gene="aroG"
                     /locus_tag="PK522_02670"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HNC40671.1"
                     /translation="MKYQTDDLRIVGMHELTPPVELHREYPVTEVATETVYTARQEIH
                     EILHGHDDRLLVITGPCSIHDVEAALEYAARLKHQREKFKQQLLIVMRVYFEKPRTTI
                     GWKGLINDPDLDNSFHINKGLRMARHLLLDLNRMGIPSATEYLDLISPQYLSDLIAWA
                     AIGARTTESQGHRELASGVSCPIGFKNGTYGNLNIAIDAIAAASRPHHFLSVTKEGTT
                     AIFATKGNEDCHIILRGGRKPNYDAASVTSAVEELQKAKLPARLMVDFSHANSHKDYR
                     RQVEVAENVAQQIAEGSHAIFGAMIESHLVEGNQKAEGKPREQLVYGQSITDACINFE
                     TTEQVLQQLAHAVDKRRKRRKR"
     gene            complement(6104..7360)
                     /locus_tag="PK522_02675"
     CDS             complement(6104..7360)
                     /locus_tag="PK522_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNC40672.1"
                     /translation="MFLKVAGYSLLYRKFSVLLTVLSVTLSTFVLLGIEHIRTEARES
                     FSNTVSGVDLIVGARTGQLNLLLYSVFHIGHATNNISWASYQALLTDQKLAWAIPISL
                     GDSHRGYRVVGTTGDFFRHYRYGEQQPLTFAAGHALVHRFDVVLGAQVAKNLGYRLGD
                     SIHLVHGIGQTQFSLHDERAFNIVGILKPTGTPIDHALYISLEGMEAIHLNWRDGVKL
                     PSHSSSDTEIDTRPLTPKAITAIFVGLQTKMATFQVQRHINDYRKEPLMAILPGIALT
                     DLWQITRTMENTLQLIAALVLLSSLLGLSTLLLSSIRERQREIAVLRAIGARPSFVFW
                     MIELEALLITLSGIALALIALVMATQSAQTVLAENYGLFITPFTLNPAALTVLGIIII
                     SALLLATIPALSAYRVSLHAGLALKQ"
     gene            complement(7350..8057)
                     /locus_tag="PK522_02680"
     CDS             complement(7350..8057)
                     /locus_tag="PK522_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC40673.1"
                     /translation="MSSPAPPQAVMLRDLQFQYPGKATTYVLDIPFWSIAQGDRLFIQ
                     GPSGSGKSTLLNLIAGILIATCGKIEVLGQDLSELSPAQRDRFRAQHIGVVFQQFNLI
                     HYLSVIDNVRLAAYFANSNLAEVTTRAKHLFAALNLNESLFTQQADRLSVGQQQRVAI
                     ARALINQPEILIADEPTSALDQATRDRFIKTLLTLSSAQNTTLIFVSHDNTLAPHFQH
                     HINLAELNRAGEAQHVS"
     gene            8155..8802
                     /locus_tag="PK522_02685"
     CDS             8155..8802
                     /locus_tag="PK522_02685"
                     /inference="COORDINATES: protein motif:HMM:NF023164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HNC40674.1"
                     /translation="MNPGLRNRNMAILLLAVMLLLVACGKESSDKPNTSALTPSTAQQ
                     ALDSSFKTIEWIDLMPQDDLDALLNPPDYLDEIEDGSEEDQLSNQFQTAIAQASDDRY
                     QQALVSTRIKPEFDNQKIRLPGFIVPLEFGKNQTVTRFFLVPYFGACIHLPPPPPNQI
                     VYGVFDKGLKVDVLHEPFWINGVLHTTLMENDIAKAAYTMTVTEIVPYVDEEIAN"
     gene            complement(8799..9365)
                     /locus_tag="PK522_02690"
     CDS             complement(8799..9365)
                     /locus_tag="PK522_02690"
                     /inference="COORDINATES: protein motif:HMM:NF022439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2796 domain-containing protein"
                     /protein_id="HNC40675.1"
                     /translation="MMKLSQFTFSQFLLGLLGWLFSGASLLAADAHHTHGAHVHGEAE
                     LYIIYEHSRLFIEFTSPAINLIGFEHPPQNEKQQNALSHAKQVLAEGKQLFQIESPHC
                     QLETATASAPYIDGTPSAEHFHPQGEHPDFHASYTFNCQANTKLTTIHTTLAAKFPGI
                     ERIKVLWIAYNQQGSTQLTANHATISLN"
     gene            9576..9848
                     /locus_tag="PK522_02695"
     CDS             9576..9848
                     /locus_tag="PK522_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNC40676.1"
                     /translation="MNKSDLIDAIAKSASISKASAGEALNGALAAIKEALKKNESVTL
                     VGFGTFKVGKRAARTGRNPRTGAEIKIKAAKVPKFAPGKALKDAVN"
     gene            9873..9948
                     /locus_tag="PK522_02700"
     tRNA            9873..9948
                     /locus_tag="PK522_02700"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9906..9908,aa:Val,seq:tac)
     gene            9984..10060
                     /locus_tag="PK522_02705"
     tRNA            9984..10060
                     /locus_tag="PK522_02705"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10018..10020,aa:Asp,seq:gtc)
     gene            10140..12026
                     /locus_tag="PK522_02710"
     CDS             10140..12026
                     /locus_tag="PK522_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNC40677.1"
                     /translation="MFDFVHRKKTIVQIILFLVMLPFLFWGIQSYRNESQSAVATVEG
                     EEIPRWEFDQAMRNQHDRLRNMLKDNFNSAMLDSPEMRLAVLEGLIQQKIMHREAEEI
                     GLTVLDSRLASEIQSISAFHDENGKFSYQRYEELLRRQQMTPVIFEDKVGAEILQQQL
                     LEPLSSSAMVADAIVQAVIRLSEVKREINQVEIAPDQYLSEVNPDEDAIKTYYDQHQA
                     EFLLPERVRIEYLVLSLNDLARQETVTSEEVRNYYEDHQSDYGQPEERLASHILIAVK
                     DTTSEEAHAEAKRKVEDILAQVTQDPDKFAELAKEFSDDSGSAKSGGELGFLGKGVLV
                     KEFEDALFQMEPNEIRGPVETAFGFHIIKLSEIKPAQVAPVEEVEDSIEEMLKRQKIT
                     SRFGEIAEEFSNIVYEQSDSLLPAADRFNLTVKQSDDWINKKSREPVEIANEKLMQAL
                     FSEDVIKEGRNTDAIEVMPDTFVSARVLEHKPATAQSLEVVRDQIVEKLKLQLAAEKT
                     VEEGSDKLARLIAGETDLVDWGDTPKTVSYMQSQGLDIETLRAIFQADVTQLPAYVGV
                     TNLQGKYNLVRINQVIEPDAADKEKLQSFSGQLKQMLLQEELAAYQASLRQRYSVKIR
                     PESY"
     gene            complement(12020..12805)
                     /locus_tag="PK522_02715"
     CDS             complement(12020..12805)
                     /locus_tag="PK522_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC40678.1"
                     /translation="MGFLANKRILITGLLSNRSIAYGIAKAMQREGAELAFTYQGADL
                     RDRVAKLANEFNSTLLFSCDVQDDQQITQCFEDLGKHWDSLDGIVHSIAFAPREALTG
                     DYLESVNREAFHIAHDVSSYSFAALAKAGLKLMEGRNAALLTLSYLGAVRVMPSYNVM
                     GLAKASLEANVRFMASSLGKKGIRVNAISAGPIKTLAASGIGNFGKLLGYAEKVAPLR
                     KNVTTEEVGNVAAFLCSDLASGITGEITYVDAGFNTVAFDLDQ"
     gene            complement(12888..13991)
                     /locus_tag="PK522_02720"
     CDS             complement(12888..13991)
                     /locus_tag="PK522_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HNC40679.1"
                     /translation="MSFHLFQINELYSNANGAIQFIELTIGNINGESFWQGQSIDVIQ
                     GNAVHSFRFTTNLPNPNTANKSVLIATQGFADLNFITPDYIVPDGFLFTSGSATVNFA
                     GADAVTYANLPINSVHSIDRNGTIQINSPKNFAGVTGTVPSNTILGTSGPDQLVGTNA
                     DNTIEAGDGNDRLNGLLGNDLLNGGTGIDTAIYSDNSSAYTINSTKDGISISGPEGSD
                     TLINMERLDFLDKNLAFDLAQGQSAGNTVRIIGAAFDANNIIPEYVAIGLQLFDSGQS
                     LLNVSELVVNSALFLSMTGSTSNEAFVNHVYQNVVGESPSATERDLYVGMLKGSGGTL
                     TQAELLILAANAVVNETNINLVGLLQNGAEFSA"
     gene            complement(14043..14879)
                     /locus_tag="PK522_02725"
     CDS             complement(14043..14879)
                     /locus_tag="PK522_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC40680.1"
                     /translation="MARPLRIEFPGSFYHVTSRGDRHEPIYLDNADRINWLALFGHVC
                     QRFNWICHTYCLMDNHYHIVVETIDGNLSKGMRQLNGVYTQAFNRRHDRVGHVYQGRY
                     KAILIEKDNYLLELSRYVVLNPVRAGIVKNVDQWPWSSYSAMIGKSDCPNWLQTKWVL
                     SQFSKQRKRAIAAYINFVHAGIGLPSIWNDLQGQIYLGKEAFIKKMQQHVQLDKNISE
                     IPRIQRSTQAKPLSYYSSFKERNTAIVAAYQTGNYTMKAIADEFGVHYATVSRIVKKA
                     EI"
     gene            14974..15420
                     /locus_tag="PK522_02730"
     CDS             14974..15420
                     /locus_tag="PK522_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNC40681.1"
                     /translation="MYQRILIPIDGSATSSCALKEAIKLAQEHSAQLALVNVYEDILY
                     WVDESYINYEELQETIRVNAEKLLAKGQTEVIQAGLPVETKLLEAKNDRIENVIIGEA
                     DRWHADLIVIGTHGRSGFSRLMLGSVAEGVARLTSVPVLLIHCAEK"
     gene            complement(15601..16374)
                     /locus_tag="PK522_02735"
     CDS             complement(15601..16374)
                     /locus_tag="PK522_02735"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HNC40682.1"
                     /translation="MRKILIHCLESIGLKVLLSEIYFHLRTIGIKKVKPQIIRSLPHD
                     STAFTQGLAYYNHTLYESTGLVGHSRLRCIDPENGNLINEIEIDGEWCEGIAVVNNRL
                     IQLTYTSGRAIVYDVNSLKPYNEFSYEGEGWGLARCGNLFLMSNGTNRLFYRDENFKI
                     ISELPVWLNQRQLNQINDIECVGDKIFANVLFENCIYEIDNKTGRVRHIIDCREIVKN
                     SGRRNAQDVLNGIAYVPSTKTFYITGKCWPMLFEILIPE"
     gene            complement(16419..17600)
                     /locus_tag="PK522_02740"
     CDS             complement(16419..17600)
                     /locus_tag="PK522_02740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC40683.1"
                     /translation="MTPSNTETITWKLVSTSSQANIDSEAFKKKMLLDQDPRWLITFV
                     TGEDKQLARFVLEQSGAIIASATFFVHPSALRIAISDWTLFSIPVKRYTLFAEIWFDP
                     TITADKSLYLLALFAQLRKELSINEVIFLQAVVSDSALSDVLAQKSSLSTSYHVLNYG
                     EPYNHRYIDFGGDYNHYLSQLSSSTRSDLKRTQKRFVSAVNGQYKTICYQSPEEVGTF
                     LDAAMAVSALTYQYQLLGAGLRDRNILENRLLTTAKLGWFHSYVLFVDQKPVAFQVGH
                     LYQECYYAQEIGYDPDWAKLQVGIFLHTEIISNLLATPDLVKRFDFGNDDNTHKQRLS
                     NAQTTEKYVYLIPRHWRNNLFVFTMKITNRLSTSVGHLLNKYGLRKRVRQLLWKLGVM
                     K"
     gene            complement(17754..18956)
                     /locus_tag="PK522_02745"
     CDS             complement(17754..18956)
                     /locus_tag="PK522_02745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC40684.1"
                     /translation="MSNTTLEWIDLASLGSEVRQPVLNQIERLIQKHPDANIDHDPYW
                     LSAKCGNDKSSKLFVCITPDNTVAGYAPFFIHPSALSFQLFGISVYDYRIRRYCITAT
                     PLLAEEYQSLSSALLEKLLITLREIIGDRDSLFGLGVEKSSNFSQFILENTELRKYYQ
                     VLPYGQSYFRRLISLPKDFEAYLKGLGNSTRYEMRRALRLLEKDTEITTSYRIFTQPE
                     EVVELLQLLQQISLKTYQHQLLNLGINDNAENRHTFELAARKGWLRSLILFSNNEPIA
                     FQHGFLYNKCFYLTHIGYDPAWAKWSVGTITHAYLIQNLIEVGAEKFDFLYGDNGSKA
                     RLSNDQREEQNFYIVPRKFPLSFIASMLQAFNFVMNTLGRAIEKFGIKTLIRRFLRRR
                     ATSSSEKN"
     gene            complement(19366..20919)
                     /locus_tag="PK522_02750"
     CDS             complement(19366..20919)
                     /locus_tag="PK522_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNC40685.1"
                     /translation="MKKLVKQFDPFRLLALILWLCPTLALFLFGLIWLWQIDGFVYWL
                     IALILCSGAGYLLQQQLIRRDRQLLSEAVTEPNPDWPPSAEIAWQKVDQLANQCDPQA
                     WSIEDASWIVELGRQTLDTVSHCYHPDAEKPYLELTVPHTLLIIEQASRDLQRDITEN
                     IPFSDRFTIGDLLRLQRWKDKAEQAINVYRAGRALVNPMHALFGEIWQHLRQRSFSLA
                     RDELYRWFLRAYIRKVGYYAIDLYSGRRPIIDKTATVPTESPPTEPQQEKTRYPSEAS
                     HPPLRFLVLGRSNTGKSSLINALFGKLTTASDILPDTTRQFSSFAIEREGMEQALIFD
                     GPGCDSQLFDEQQMIIAALNADLIFWVTAANRPDRQDERRFLDHLRAEQLKRLNHRPP
                     SIIVVVSHIDLLRPASVWQPPYNLIDPTDTKAMNIRAAMQFIATDLAIPIEQIIPVCL
                     KPGREYNVQDTLWACILDQQDVAMRVRLLRCLDARKQTEDWALLRRQMISAGRFLWSL
                     PKSNEKRVI"
     gene            complement(20923..22137)
                     /locus_tag="PK522_02755"
     CDS             complement(20923..22137)
                     /locus_tag="PK522_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HNC40686.1"
                     /translation="MKLSKITDWLTYWQKLQTILADPKIDEAALDASLRQVRQKIPLP
                     VFWLIGKTQSGKTSIIHALTGSEAAEIGNGLQPCTRFSSFYDFPAQMPVVRFLDTRGL
                     GEIDYDPSEDIHYCESQAHLLIAVMKVADLQQQIVLKVLQTVRTRHPDWPIILVQTGL
                     HELYGPHDQHLTPWPFDQDPLPNEVPTDLQRALVAQRQTAIALPGSAPMLWVPVDLTL
                     PEDGFSPTHYGLEPLWKAIELVLPLGLQRQLAGEKEIQDFFARTAHQHIVGYSLTAAG
                     LGALPAVDLVMVTTLQAKLLRDLAKLYGQKWNKQTTIEFFSLLGTAITSSYFVRMIGR
                     TLTKLIPGVGQTVGAVWGASASAATTYALGKAAVYFFTQRQNGLNINPELLRKAYAEA
                     LEASASLLKQRI"
BASE COUNT         6214 a         4653 c         5378 g         6114 t
ORIGIN      
        1 ggcagggcag tggccagaat cgttgaaacc gggttttgaa cccgcacgct tgcaatggct
       61 gatcgatcaa gagcaatccc agcagcggtt gcaatggtgg gtattacaaa ccagcgcaca
      121 aggctatttt gtttgccaac ccggttcacc agaaacaacc tcaccgcaat tacccagcgc
      181 ggtgttggaa tcattatccg ccattccacc gacctatcag gttttatcgg gcagcacact
      241 atcacggcgt ggtattgtac gtccagcgca agtgttctct gcggctgatg caccactggt
      301 tgtggaaacc agtatgcaac gggtcgaact cgaagcgatt caaaagccag cctgggcatt
      361 acgcatcaaa gccaatcgtt cagggttggc ggttgattta ccggtttggc aaggaaaatc
      421 agtcactgcg ccttggcagc cgggcactgg atcgagcgcg gggcgttggc aattgccagt
      481 accgttcggt ttggacgaat ggggtcttta tgcggatctg acggtcagag aagtgacaca
      541 acgttttcgc tggattgtgc cgggcacatt tacgatgggt tcaccggaaa acgaagcaga
      601 acgaagagaa gatgaaacgc agcatcttgt cacattgacg caaggctttt ggctggcgga
      661 tagcgtttgc acgcaagcat tatggcaagc agtgatgggg ggtgataatc cagcccattt
      721 ccgggattca cccaatcatc cggtagaaca agtcagttgg aacgatgtgc agcagtttat
      781 ccaaaccctc aatgcgcagt ttccagatat acaagcacgg ttaccgaccg aagcggagtg
      841 ggaatatgcc tgccgcgcgg gcacgacgac ccccttctcg tttggcggca atattacgtc
      901 cgaacaagtg aattatcatg gtgaatatcc ttatgctgga gctgagaagg ggaggtatcg
      961 agagaaaacg gtaccggtta aatcattgcc ccccaatccc tggggattat acgaaatgca
     1021 cggcaatgtt ctggaatggt gtggcgattg gtatggcact tatccagccg aagcagtggt
     1081 agacccaata gggcccgtta ccggacaagg ccgcgtgttg cgcggcggcg cctggggcgg
     1141 gcctgacctc cgtagcagcc tctttggctt ccggcttgcc ctagggcata agcctgccag
     1201 taaggccggt aaggctggtg agacaggccg tcaaggcacc gatagggagg catgatgaat
     1261 ccacagctac taccgcctgc ctttcctact tcctgggcca atgaatgggg tgaggatgaa
     1321 ttcggcttat acctcgattg taatgttaac ggaatcgtgc aacgctttcg ctggattgtg
     1381 ccgggtacgt ttacaatggg ttcacctgaa aacgaagcgg aaagagaaga agatgagaca
     1441 cagcattttg ttaccttgac acagggattc tggcttgccg atagcacttg cactcaagcg
     1501 ttatggctag ctgttatggg aaataatcct actttcccta ttattatagg agaaaattta
     1561 ttcgataatt tatcggataa agtgaaagca acacagcagc aattattttc taatgatgat
     1621 ttagatcctt cactcgtcca tccggtaaca cgagtgagct gggatgatgt acaaaaattc
     1681 atccaaacga ttaatgcaca gttaccggat ttgcaggcaa ggttaccgac cgaagcggag
     1741 tgggaatatg cgtgccgtgc gggcacgatt acgcccttct cttttggcaa caatattaca
     1801 cccgaacaag taaattatca tggtgagtat tcttatgctg gcgctgagaa ggggctacat
     1861 cgtggaagaa ctgtaccggt taaatcattg ccccctaatc cctgggggtt atacgaaatg
     1921 cacggtaacg tttgggaatg gtgtgcggat cggtacaccg cttatccttc atttgcagtg
     1981 atcgatcaat cagggtcagc tactaaagaa tatcgttcat tgcgtggtgg atcgtgggcc
     2041 agccatgctg gtagggttag atctgctttt cgtgatggag atgaagctaa ttttaacgat
     2101 ggtgttattg gtttccggct tgccctcggt cgaaaggcga gcacaagcca ggtgtgagtc
     2161 agcggcagcc gagtggccgt agcggagcaa ggcctagcgc tgccgcacag tattaaatca
     2221 gctattccgc catgaggatg ttagcgccaa aacaaagcat tacaaaccga tgccatcgca
     2281 ttgagatcgc ataaaacctg cgtataatga ggacaggtaa cccatcgata caagtggagt
     2341 ggaatagtat gtcaggcatt tttatcagct accgacgcca tgatagccaa agcgcagccg
     2401 gtaggttggc tgatttttta gagcgcagct ttggggcgga tcaaattttt cgcgatgtcg
     2461 aaacaatcga acccggggtc gattttgtac aagcgatcga aaaggcgatt gcctcttgcc
     2521 aagtcatgct ggtggtgatt gggccacgct ggctcacgat taccgatcaa cagggcaatc
     2581 gtcgtttgga taaagccgat gattggattc gcatcgaaac taagttggcg ttgcagcgca
     2641 atatccgcgt gattccggtg ttagtggaga atgcgcaacc cccgaccatg gacgcattac
     2701 ccgatgaaat caaatcgctg gcgcggcgtg aagcgcatga actcagcgat aaacgctggg
     2761 attatgatgt gcagtgtttg atcgaagcgc tggaaaagat tcccggtatt cgtcgcaaag
     2821 tacaaacgac gaccttttca cagaatgcac cggtgcaacc ggttaccact cgtttgacga
     2881 cgaaattggg taaagtgcta gcctggattg gtggaatttt cgtggcgctg attatgatcg
     2941 gcgtattctt ggatgacacg acgacatcga taaccccacc gttatcgccg ccgctcacgc
     3001 cggttacacc gcaaccgttg atgcaaggat taccttccgg ttcgccaact tcgacgtgtg
     3061 ggtgttgggg ttatgtggaa gtcggcgcga cacgtcccaa tccgcagtgc gcttccggta
     3121 tcgatactgt ggtgatgtgt caaggcgttt gtgcgggtgg tggcgcgcct tggcaatcgt
     3181 tgtgccaata gtaccttaaa ggcgctacta cgcttttttg atcgcgctga agctgaataa
     3241 ccctaccaaa agccatttta tttcagcctt ttagcgcaac atttttgcgt agtattatct
     3301 cctatgattg actttagaga gtgtcgtgct ggattttgca ggcggccgta ggagtgtgac
     3361 atggataaga gtgcattact cagcgcattg gcggctgaaa ttgagcaagg gaaaatgatt
     3421 tttccaactt cgaccagtgc agcgatcaat atcaaaaaaa tgctggatga tcctgattgt
     3481 gagttggata aagtgacgcg attggtacaa gctgaaccgt tgctagcaac caaagtagtg
     3541 gcagtggcta actcggtggt gttcaaccgc tccggtaaac gtgtcaccga tgtacgttca
     3601 gcgattagct tgttgggttt taacatggta cgcaatttgg caaccgcgat tttgacacgt
     3661 cagcttgcgg gtaaggcagc caatagcgaa gtcgttaccc agttatggaa gcattcggcg
     3721 tatgtggcat cgctggcgca agtaatcgcg cgccgcgtta cgaaacaaaa ccccgatact
     3781 gcgatgtttg ccggcatgat tcatgaaatc agttggtttt atttgttgtc gcgtgaaaaa
     3841 gattttccag ggctaaccga tggcaatttt gcgagttctt gggtgagcga tgaagatttg
     3901 gatgatgaaa ctgaaatcga gtgtgaaatc aaaatcggta ctgcgatcct caaagcgctt
     3961 gccgtgccag cgccggttgt tgagggtatt acttgcttgt gggaaggata tttggcttat
     4021 ccaccgacga cgctcggcga taccttgctg tttgccgatc agttatcccc aatccgttca
     4081 ccgtttgtat tgccgtcaga tgaaaccggc gaggacatca gagcaaaaat ggatacactg
     4141 accgatcaag aaacgttgag cgagattctc agagattcag aagaagaagt taaatcgctt
     4201 accgaagcgt taggcactta gtttttgatt acacgctgag cgctgccttt aatcagcgct
     4261 tcaacctgca atcgtgttct tttcttcagt gataaatctt ggaatgaaac gtgtcgcgta
     4321 gcagtcgcag gccaaacaat acaaactgca agccaatgcc cgcaatgacg gcggcagtta
     4381 tccaaggacc gatttgatcg acgatagtgg ctaataattg cccactggga ctacccggta
     4441 acggttgctc catcatgata tttaccgcaa catagcacgc gagcaagcca aaacaaccaa
     4501 tcaagctgcc agtaatgcct gggcgtagtc gatagtttgt accgaaggaa ttacgaatcg
     4561 agctgcgcct agcgagcatc agtaacagta caaatagtac gattcctgta ataatcatgg
     4621 cagcatcggg ttcaaaaacg aattgcagca ccgcaggata atacgctagg taaacataag
     4681 acagaaatgc attgagaagc acactaatga agctcataac acacctcctg ataggaatgg
     4741 attgttagca tggagtgccg tcatcgcatc gatcgccagg atagaggttg tttgatagcc
     4801 ccgttatcat cggatcgagg gtggtttgat gctagcaaaa ttcccaaccg aggcatgtga
     4861 caaaaaacac acgaattcga cgataaattg tccttaagtt tccaaacttt tgttgagcgt
     4921 tatcaaaatt tcccttcgcg cttagaatcg cctctaggtt aaaataggcg ctttatttcc
     4981 aactgatcaa tcgctggttt ttagccagtt tgtttaaaca agggaaacac catgaaatac
     5041 cagaccgatg atttacgcat tgtcggcatg catgaattaa caccgcccgt ggagttacac
     5101 cgggaatatc ccgtgactga ggtcgcaact gaaaccgtat ataccgcacg gcaagaaatc
     5161 catgagattt tgcatggcca cgatgatcgc ctattggtga ttacagggcc ttgctcgatc
     5221 catgatgtag aagctgcgct ggaatacgca gcacgcttga aacaccagcg tgagaaattc
     5281 aaacagcaac tgttgatcgt aatgcgggtt tatttcgaaa agccgcgtac gaccatcggt
     5341 tggaaaggat tgatcaatga tcccgatttg gacaatagtt ttcatatcaa taaggggctg
     5401 cggatggcgc ggcatttatt gctcgatttg aatcgcatgg ggattccctc tgctaccgaa
     5461 tatctggatt tgatcagccc acagtatttg tcggatttga ttgcttgggc ggcgattggc
     5521 gcacgtacca cggaaagcca aggacaccgt gagctagctt ctggcgtttc ctgccccatc
     5581 ggattcaaga atggtactta tggcaatttg aatattgcta tcgatgcaat tgcagccgcg
     5641 tcgcgcccgc accatttttt gtcagtgacc aaagaaggga ccaccgcgat ttttgcgacc
     5701 aagggtaatg aggattgtca catcatttta cggggtggtc gtaagccgaa ttatgatgct
     5761 gcaagcgtga cctccgctgt cgaagaattg cagaaagcaa agctaccggc gcgcttgatg
     5821 gtggatttta gccatgccaa cagtcacaaa gattatcgtc gtcaagtgga agtcgctgag
     5881 aacgttgccc agcaaattgc cgaaggttcc catgcgattt ttggtgccat gatcgaaagc
     5941 catttggtcg aaggaaacca aaaagcagag ggtaaaccgc gtgagcaatt ggtgtatggt
     6001 cagagcatta ccgatgcttg catcaatttt gaaaccactg agcaagtatt gcagcagttg
     6061 gcgcacgcag tggataaacg tcgtaagcgc cgcaaacgtt gatttattgc ttaagggcga
     6121 gcccagcgtg caatgaaacg cgataggcgc tcaaggcagg gatggttgcc aatagcaaag
     6181 cactaataat tatgataccg agcacggtga gtgcggcagg attgagtgtg aaaggggtga
     6241 tgaatagacc gtaattttca gccagaaccg tttgcgccga ttgggttgcc attactaatg
     6301 caatgagcgc aagcgcgatg ccgctgagcg taatcaacag tgcttccagt tcgatcatcc
     6361 aaaacacgaa agacggcctt gcaccaatgg cacggagcac ggcgatttca cgttgtcgct
     6421 cccggatcga tgatagtagc agcgtgctca agcctagcag tgatgaaagc agcaccagtg
     6481 cagcaatgag ttgtagggtg ttttccatgg tgcgggtgat ttgccataaa tcggtgaggg
     6541 caatgcctgg caagatcgcc atgaggggtt ctttgcgata atcattgatg tggcgttgca
     6601 cctgaaacgt ggccattttg gtttgcaggc cgacgaagat ggcggtgatc gctttcgggg
     6661 tgagcgggcg agtatcgatc tcggtatccg atgagctgtg actgggaagc ttgacgccat
     6721 cgcgccagtt taaatgaatc gcctccatgc cttccagact gatatataac gcatgatcga
     6781 tgggggttcc cgttggtttc agaattccca cgatattaaa agcccgctca tcgtgcagac
     6841 tgaactgagt ctgcccaata ccatgcacta aatgaattga atcaccgagg cgataaccga
     6901 ggttttttgc aacctgtgca cctagcacga catcgaatcg atgaaccagt gcatgacctg
     6961 cagcaaatgt tagcggttgt tgctcaccat aacggtaatg cctaaagaaa tcaccggtag
     7021 tacctaccac gcgatagccg cgatgagaat cacccaaaga aatgggaatg gcccaagcaa
     7081 gcttttgatc ggtgagtaac gcttgataac tggcccagga aatattgttg gtggcatggc
     7141 caatatggaa tactgaataa agcaataggt tgagctgccc ggtgcgcgcg ccgacgatca
     7201 gatcgacacc ggagacggtg ttgctaaagc tttctctagc ttccgtgcgg atatgttcaa
     7261 tgccgagcag tacaaacgtg ctgagggtga cggacagtac cgtcaataat actgaaaatt
     7321 tacgataaag caggctatac cccgcaactt taagaaacat gttgagcctc tccggcgcga
     7381 ttgagttcag ccaaattgat gtgatgctgg aaatgaggcg caagcgtgtt atcgtggctg
     7441 acaaaaatca atgtcgtgtt ttgtgcgctg ctgagggtaa gtaatgtttt aatgaaacga
     7501 tcgcgcgttg cttgatctag ggcagaagtg ggttcatctg cgatcaatat ttcaggttga
     7561 ttgatcaatg cgcgggcaat ggcaacccgt tgttgttgac cgacgctgag tcgatccgct
     7621 tgttgtgtaa ataatgattc attaagattc aaggcggcaa ataggtgttt tgcacgggtt
     7681 gtaacctcag caaggttgct attcgcaaaa taagcggcta aacggacgtt atcgatcacg
     7741 ctcaggtagt gaatcagatt gaactgttga aagactacac caatatgttg agcacggaat
     7801 cggtcgcgct gcgcgggtga caattccgat aagtcttggc cgagtacttc gattttaccg
     7861 cacgttgcga tcaatatgcc ggcgatgaga ttgagtaatg tcgatttacc tgagccggaa
     7921 gggccctgga tgaagagtcg atctccctga gcaatcgacc agaaaggaat atcgagcacg
     7981 taggttgttg cttttccagg gtattggaat tgcagatcgc gaagcataac ggcttgaggt
     8041 ggcgcagggg atgacacgga atcgactcct tccttaacac attttccagt gggtttaatt
     8101 cgttcgatta gcaaggatga tatactggct gctcaatatt caaaatgctt ttttatgaat
     8161 ccaggcttgc ggaatagaaa catggcgatt ttattgctcg ctgtcatgct actgttggtt
     8221 gcttgtggga aagaatccag tgataaaccg aacacatcag cacttacgcc aagcaccgcg
     8281 cagcaagccc ttgactcgtc gttcaaaact attgagtgga tcgatctgat gccgcaggac
     8341 gatctcgatg cattattgaa tccgcccgat tatttggatg agatcgagga tggttcggaa
     8401 gaagatcaac ttagcaatca gtttcaaact gcgattgcac aggctagcga tgatcgttat
     8461 cagcaagcat tggtatcgac gcgcatcaag cctgaattcg ataatcaaaa aattcgtttg
     8521 ccgggtttta ttgtgccatt ggaatttggt aagaatcaaa cggtaacgcg gtttttttta
     8581 gttccttatt ttggagcctg tattcattta ccgcccccgc ccccgaatca gatcgtttat
     8641 ggggttttcg ataaaggatt gaaagtcgat gtgctacatg aacccttctg gatcaacgga
     8701 gtgctgcaca ccacgttgat ggaaaacgat attgctaaag cagcttatac catgactgtt
     8761 acagagatcg tgccttatgt cgatgaggaa atcgctaatt aattgagcga aatcgtcgca
     8821 tgatttgcgg ttaattgtgt cgatccttgt tgattgtatg cgatccatag cactttaatg
     8881 cgttcaatgc ctggaaattt tgctgctaac gttgtatgga tggtggtaag ttttgtgtta
     8941 gcttggcagt tgaatgtata actcgcatga aaatcagggt gttctccctg cggatgaaaa
     9001 tgttcagcac tgggcgtacc atcgatataa ggcgcgcttg cggttgcggt ttctaattgg
     9061 caatggggcg attcaatttg aaataactgc ttaccctcgg ccagaacctg cttagcgtga
     9121 gacaaggcgt tctgttgttt ctcattttga ggagggtgct caaagccgat aagattgatg
     9181 gcgggggaag tgaattcgat gaataatctg ctatgctcat agatgatata aagttcagct
     9241 tctccatgaa catgcgcgcc atgcgtgtga tgggcgtcag cagctaatag gctagcgccc
     9301 gaaaacagcc agccgagtaa acccaataaa aactgtgaga aagtaaattg gcttagtttc
     9361 atcattgcgg tgattgatta acgataggtt aatgatatga taatttgagc ctagattcag
     9421 gtggttgttc aaattcttac caagaccact aagattttta agaatttgtt aggtaacact
     9481 tgaccatgtt caaatggctt gatataaggg ggtttgcaga atcttctgtt aacgatttac
     9541 tactaataac ccaataaacc gaaaagggga tttaaatgaa taaatctgat cttattgatg
     9601 cgattgcaaa gtctgccagt atttcaaaag cttctgcagg tgaggcgctt aacggtgcat
     9661 tggccgcgat taaagaagca ttgaagaaaa atgaaagtgt aacattggtt ggttttggaa
     9721 catttaaagt aggaaaaaga gctgcgcgaa ctggccgcaa tccccgtacc ggtgccgaga
     9781 taaaaattaa agcagcaaaa gtaccaaaat tcgcccctgg taaagcgctt aaagatgcag
     9841 taaactagca acctatatcg attaagaaaa aagggtgctt agctcagctg gtagagcgtc
     9901 gcccttacaa ggcgaatgtc ggcggttcga tcccgtcagc acccaccatt aaaggtggct
     9961 tgccaaatat aaaattataa gttggagtgg tagttcagtt ggttagaata ccggcctgtc
    10021 acgccggggg tcgcgggttc gagtcccgtc cactccgcca ttttttcagt cagctgttta
    10081 aagctgataa gtgttaacgc tccaatatac ttccattact tcttaattag gttctagtcg
    10141 tgtttgattt tgtccatcgc aaaaaaacga tcgttcagat tatcctcttt ttagtcatgt
    10201 tgcccttttt gttttggggc atacagtcgt atcgcaatga aagtcagagt gcggttgcca
    10261 cggttgaagg tgaagagatt ccccgttggg aatttgatca agcgatgcgc aatcaacatg
    10321 atcgcttacg taatatgctc aaggacaatt tcaatagcgc catgctggac agcccagaga
    10381 tgcgcttagc cgtattggaa ggattgattc agcaaaagat aatgcatcgc gaggctgaag
    10441 agattggact tacggtttta gattctcggt tagcgagtga aattcaatcg atttctgctt
    10501 ttcatgatga aaatgggaaa ttttcttatc agcgttatga agagttatta cgtcgtcagc
    10561 agatgacgcc tgtcattttt gaggataagg tgggtgctga aattttacaa cagcagcttc
    10621 tggaaccact ttcgagtagc gcaatggttg ctgatgcgat tgtgcaggca gttatccgtt
    10681 tgagcgaagt taaacgtgaa attaatcaag ttgaaattgc gcctgatcaa tatttgtccg
    10741 aggtgaaccc ggatgaggat gcgataaaaa cctattatga tcaacatcaa gctgaatttt
    10801 tattaccaga acgggtgcgt attgaatatt tggttctgtc acttaacgac ttggcgcggc
    10861 aagaaaccgt aacttcagaa gaagttagaa attattatga agatcatcaa agcgattacg
    10921 gtcaacctga ggaacggtta gcgagccata tcctgatcgc agtaaaagat acgacatcag
    10981 aagaagcaca tgcggaagct aaacgtaaag tagaagatat tttggctcaa gtcacgcaag
    11041 accctgataa atttgctgaa cttgctaagg agttttccga tgatagcggt tctgcaaaat
    11101 cgggtggtga actgggcttc ctaggcaaag gtgtgttggt gaaagaattt gaagatgcgt
    11161 tatttcaaat ggagcccaat gaaattcgcg gaccggttga aactgctttt ggatttcata
    11221 tcatcaagct ttctgaaata aaaccagctc aagtcgctcc agttgaagaa gtagaagata
    11281 gcatcgaaga aatgttgaaa cgccaaaaaa ttaccagtcg atttggtgaa attgctgagg
    11341 aattttctaa tatcgtatat gagcaaagcg atagcctatt gcctgcagca gatcgtttca
    11401 atttaacggt gaaacagtca gatgattgga tcaataaaaa atcgagagaa ccagtagaaa
    11461 ttgcgaatga aaaacttatg caagcgcttt tctctgaaga tgttatcaaa gagggacgga
    11521 ataccgatgc aattgaggtg atgcccgata cctttgtttc tgctagagtg ctggaacata
    11581 aaccagcaac tgcgcaatcg ttggaagtgg ttcgggatca gattgttgaa aagctcaaac
    11641 ttcagctagc tgctgagaaa actgtagaag aagggagtga caaattggcc cgactcattg
    11701 cgggagaaac agatctcgtt gattggggtg atacaccgaa aaccgtttcg tatatgcagt
    11761 cacaaggatt ggatatcgaa acgttacgtg cgatttttca agcggatgta actcaactac
    11821 cggcttatgt cggtgttact aatctgcaag gcaaatacaa tttagttcgt attaatcaag
    11881 tgatcgaacc tgatgctgcc gataaagaaa aattgcagtc ttttagcggc caactcaagc
    11941 aaatgttatt gcaggaagag ttggcagcgt atcaggcaag tttgagacaa cgttattcag
    12001 taaagatacg acctgaaagc tattgatcta agtcaaatgc gacagtattg aatcctgcgt
    12061 cgacataagt gatctctcca gtaattccgc ttgcgagatc actgcataaa aaagctgcga
    12121 cattacctac ttcttctgtc gtcacatttt tgcgaagtgg cgcaactttt tcggcatagc
    12181 cgagcagttt tccaaaattt ccaatacctg aagcagctaa cgttttgatc ggacccgctg
    12241 aaatggcatt aacgcggatt cctttcttac caaggcttga ggccatgaaa cggacattgg
    12301 cttctaggct agctttggca agccccatta cgttataact cggcatgact ctcacggcgc
    12361 ccagatagct caatgtgagg agcgccgcat ttctaccttc cattagcttt aatcctgctt
    12421 tggcaagtgc tgcaaaacta tatgaactga cgtcatgcgc gatatggaaa gcctcacgat
    12481 tgacgctttc gaggtaatcc cctgttaatg cttctcgcgg tgcgaaggca attgagtgca
    12541 cgataccatc gagactatcc cagtgtttac cgagatcttc aaaacattgt gtgatttgct
    12601 gatcatcttg cacatcacag gaaaatagca gtgtactatt aaattcgtta gcaagctttg
    12661 ccacacggtc gcgtaagtct gcaccttggt aggtgaaagc aagctcagca ccttctcgtt
    12721 gcattgcctt ggcgatacca taagcgatag agcgattgct gagtaatccc gtaattagta
    12781 ttcgtttatt tgctagaaac cccatactca taccttttta attgataatc gaatcattat
    12841 agcgcaacgt tcctagaatg cgaggctctg cgaaatcatt gtagaactta agcgctaaac
    12901 tcagctccat tttgaagtaa tccaacaaga ttgatgttgg tttcattgac gactgcattg
    12961 gcagcaagaa ttaataattc tgcttgcgtt aatgtgccgc cactgccttt aagcatgcca
    13021 acatagagat ctcgctctgt agcagatggt gattctccaa ctacattttg ataaacatga
    13081 ttaacaaagg cttcgttact ggttgaacca gtcatagaca ggaaaagtgc agaattgaca
    13141 accaattcac tcacgttgag caatgattga ccgctatcaa ataattgcaa gccaatggcg
    13201 acatactcag gaatgatatt gttagcatca aaagcagcac caatgatgcg cacggtgttg
    13261 cccgctgatt ggccctgtgc aagatcaaaa gccaaattct tgtcgagaaa atcgagccgt
    13321 tccatattga taagggtatc gctaccttcg ggtcctgaaa tgctaatacc gtctttcgtt
    13381 gaattaatag tatatgcgct gctgttatca ctgtagatag cggtatcgat tccggttccg
    13441 ccgttgagca gatcattacc gagtagcccg tttaatcgat cgtttccatc gccagcttca
    13501 atcgtattgt ctgcattagt tccaacaagc tgatcgggtc cacttgttcc aagaatagta
    13561 ttactgggta cggttcccgt cacgcctgca aaatttttgg gtgagttgat ctgaatcgtt
    13621 ccattacgat caatggaatg aacgctatta attggcaggt tagcataggt gactgcatca
    13681 gcacctgcaa aattaacagt agcgctacca ctcgtaaaaa gaaatccgtc aggtacgatg
    13741 taatctggtg tgatgaagtt taaatctgca aagccttgag tggcaatgag taccgattta
    13801 ttcgctgtgt taggattagg taaattggtg gtaaatcgaa aagaatggac tgcattgccc
    13861 tgtattacat cgatagattg accttgccaa aatgattcgc cattgatatt accaatcgta
    13921 agctcaataa attgaatcgc gccattagca ttcgaataaa gttcattgat ctgaaacagg
    13981 tggaatgaca tattatctcc ttctaaaaag atatggggtc agatcttgca ttaatataga
    14041 cactatattt ctgctttttt aacaatacga ctgactgtag cataatgcac gccaaattca
    14101 tctgcaattg ctttcatagt gtagttgcct gtttgatagg ctgcgacgat cgctgtattt
    14161 ctttctttaa acgatgaata gtatgaaagt ggtttggctt gagtacttct ttgaatacgt
    14221 ggaatttcac tgatattttt atcaagctga acatgttgct gcattttttt tataaatgct
    14281 tccttgccaa gataaatctg tccttgcaaa tcattccata tgcttggtag tccaataccc
    14341 gcgtgtacaa aattgatata agcagcaatc gctcgcttac gttgtttgct aaattgactt
    14401 aatacccatt tcgtttgaag ccagttgggg caatcgcttt ttccgatcat ggcagaatag
    14461 ctgctccagg gccattgatc gacatttttt acaatacctg cccgcactgg attcaataca
    14521 acataacgcg ataattcaag cagatagtta tccttttcaa tgagaatggc tttatagcgt
    14581 ccttgatata catgtccgac acgatcatgc ctacgattaa aggcttgcgt ataaacgcca
    14641 tttaattgcc gcattccctt ggacaaattt ccatcgatcg tttctacaac gatatgatag
    14701 tgattatcca tcagacaata tgtgtggcaa atccagttaa accgttgaca aacatggcca
    14761 aacaaagcaa gccagttgat tcgatcggca ttgtcgagat aaatcggctc atggcgatca
    14821 cctcgcgaag ttacatgata aaagctgccc ggaaactcaa ttcttaaagg tctggccata
    14881 actatattaa aattttatta ttgtgtagta gattatatcg ttaattattt tatctcaaga
    14941 cctgacccca ttttgcttta ttggagaaaa gctatgtatc aacgaatcct tattccgatt
    15001 gatgggagcg ctacatcaag ttgtgcgtta aaagaagcga tcaagttggc gcaagaacat
    15061 tcagcacagt tggcacttgt aaatgtttat gaagatattc tttattgggt tgatgaaagt
    15121 tatattaatt atgaagaatt gcaggaaacg attcgggtaa atgcagaaaa gcttctggct
    15181 aaggggcaga cagaagttat tcaggcgggg ttgcctgtcg aaacgaaatt gcttgaagca
    15241 aaaaatgatc gaattgagaa cgtgataatc ggtgaagcag atcgttggca tgctgacttg
    15301 attgtgatcg gcacgcatgg acgctcaggg tttagtcgtt taatgctagg aagtgttgcg
    15361 gaaggcgttg caagattaac gtcagttccg gtgttattaa ttcactgtgc tgaaaagtga
    15421 ctgataaaaa tattgataaa gtacaggcta tgtgtagtag ttgcgacgat ctatcgaatc
    15481 cctcggatag atagtgtgtt tgataataaa ataggagctt taccttagat tactggtaaa
    15541 tttcaataca aaattctggg agagtctcgg aagatcattt atttagttct accagtcgca
    15601 tcactctggg atgagaatct cgaaaagcat tggccagcat tttcccgtaa tataaaacgt
    15661 tttagtagaa ggcacgtacg cgatcccatt gagtacatcc tgtgcattac gacgtccaga
    15721 attttttaca atctcacgac aatcaatgat atgacgcacc cttcctgttt tattgtcgat
    15781 ctcgtagata caattttcga aaagtacatt cgcaaatatt ttatcgccga cgcattcaat
    15841 atcgttgatc tggtttagtt gacgctgatt aagccaaaca gggagttcag agatgatttt
    15901 gaaattctca tcacgataaa acaaccgatt tgtaccattg ctcatcagga ataaattacc
    15961 gcagcgcgcc aagccccaac cttcaccctc atatgaaaat tcgttatagg gctttaagct
    16021 gttaacatca taaacaatcg cgcgtccaga agtgtaagta agttgtatta aacgattatt
    16081 aacaactgcg atgccttcac accattcccc atctatttca atttcattaa ttaggttgcc
    16141 attctcagga tcaatgcatc gaagtcgaga atgcccaact aaaccagtgc tttcataaag
    16201 cgtatggttg taataagcaa gtccttgagt aaaagcggtt gagtcatgcg gcaatgatct
    16261 tatgatttga ggttttacct tcttgatacc tatcgttcgg agatgaaaat agatttcact
    16321 caggagcact ttaagaccga tcgattctaa gcaatgaatc agtatttttc gcatcagtta
    16381 agagaatctt gctaattatg taacgataat gatatttgct atttcattac gcctaatttc
    16441 cacagcagtt gccgaacgcg tttacgcaat ccatacttat taagtaagtg gcctacactt
    16501 gtagataatc gatttgttat tttcatcgta aatacaaaga gattatttcg ccaatgacga
    16561 gggataagat aaacatattt ttcggtcgtt tgggcattac tgagtctttg tttgtgcgta
    16621 ttatcatcat tcccaaaatc aaaacgctta actaaatcag gggttgcaag caaattagag
    16681 ataatttcgg tatgtagaaa aatacctact tgtaattttg cccaatctgg atcgtatcca
    16741 atttcttgtg cgtaataaca ttcttggtat aagtgaccaa cttgaaatgc aactggtttt
    16801 tgatcaacaa acaaaacata gctatgaaac caaccgagtt ttgctgttgt taaaaggcga
    16861 ttttcaagaa tattgcggtc acggagacct gcgcctaata actggtattg ataggttaga
    16921 gcggatacag ccatcgctgc gtctaagaat gtgccaactt cttcaggtga ttgatagcag
    16981 atcgttttat attgaccatt gacagccgaa acgaatcttt tttgagtacg tttgagatca
    17041 cttcgtgtag acgagcttaa ttggctcaga tagtgattgt aatcaccgcc gaaatcaata
    17101 tagcgatgat tgtacggttc cccgtaatta agaacgtgat agctggtact taatgatgat
    17161 ttctgagcta gcacatcaga taatgcagaa tcactaacca cagcctgtaa aaaaattact
    17221 tcgttgattg atagttcttt tcggagctga gcaaataacg ctaacaaata taaagattta
    17281 tctgcggtaa tagttggatc gaaccaaatt tcagcaaaca gtgtatagcg ttttacgggt
    17341 atgctaaata atgtccaatc actaatcgcg atgcgaagtg ccgaaggatg tacaaaaaaa
    17401 gtagcactag cgattattgc gccagattgt tcaaggacaa atcttgctaa ttgcttatct
    17461 tcaccagtga caaaggttat taaccagcgc ggatcctgat caagtaacat ttttttctta
    17521 aaggcttctg aatcaatatt cgcttgcgaa ctagttgaga ctaacttcca agttatcgtt
    17581 tctgtatttg atggagtcat aactacgtat agaaatttat taagttttaa tggtaacgat
    17641 taaattaagt ttaaattttg ttgagtttta atgatttaat cttattcgcc agaaaacaca
    17701 tccaaactag ccttatccat ttttgatcta gcgcatctat ttttaaatag aatttaattt
    17761 ttttctgaag aagacgttgc gcgacgtctt aggaaacgac gaattagcgt tttgatgccg
    17821 aatttttcta tggcccttcc taacgtattc atcacaaaat taaaagcttg aagcattgat
    17881 gcaatgaaag aaagcggaaa tttacgggga acaatataga aattctgttc ttcacgctga
    17941 tcgttactta gccttgcttt gcttccatta tcaccgtata aaaaatcaaa tttttcagca
    18001 ccaacttcga ttaaattttg aattaagtaa gcatgtgtaa ttgtacccac agaccactta
    18061 gcccaagctg gatcatagcc aatatgcgtt agatagaagc atttgttgta gagaaagcca
    18121 tgttgaaatg cgatgggctc attgttgcta aaaagtatca agcttcttag ccaacctttt
    18181 ctagctgcga gctcaaaggt gtggcggttt tcggcattat cgttgatgcc taaattaagg
    18241 agctgatgct gatatgtttt taatgatatt tgctggagta gttgcaaaag ttctactact
    18301 tcctcgggtt gagtaaagat gcgataggat gtggtgattt ccgtatcttt ttccaataac
    18361 cgtaaagcac gtcgcatttc gtaacgagta ctattaccca gtccttttaa ataagcctca
    18421 aaatctttag gcaaagaaat caaacgccta aagtaggatt gtccataagg tagaacttga
    18481 tagtatttac gaagctcggt attttctaat atgaactggc tgaaattaga agacttttct
    18541 acacctaagc caaacaaact atcacgatcc ccaataattt cgcgtaatgt tataagtagt
    18601 ttttctaata aagctgagga aagggattgg tattcttcag ctagtagcgg ggttgcagta
    18661 atacagtagc gacgaattct atagtcgtat acagaaattc caaatagctg gaagcttagt
    18721 gcagatggat gaatgaaaaa aggtgcgtag ccagccactg tattatcggg agtaatacat
    18781 acgaatagtt tgctagactt atcattccca cactttgctg agagccaata gggatcatgg
    18841 tctatgttag catcagggtg tttttgtatt aagcgttcaa tctgatttaa aacaggctga
    18901 cgaacttctg accccaatga cgctaaatca atccattcta gagtggtatt actcaaaata
    18961 atccttaaat tttgatttgc taaaacaatc tttcaaggta gtaattaatc tcgctgcaaa
    19021 ggtacgatca gccggaatag attattattg catttaaaca agctcagccc cacgattagt
    19081 agtattactg caagtatgca ttaaattaac aagcaagtaa gattagaata acccagtttc
    19141 atgatagtaa ttcaagttag gctcagttca gagtaatgcc caaggcgcta cgctaataca
    19201 taattatttt acatccttcc agcttgattg ctacgaaact taggactagg tattcttgta
    19261 agtagtcgga taacacactg tgatatgttc ccaatatatc ttgagattac ccattgcgat
    19321 attaacccat gacagtgatt ttcccgcttt gtttggtggg acaagctata tcacgcgttt
    19381 ttcgttgctt ttgggaagac tccataagaa tcgacctgcg ctaatcattt gccggcgcag
    19441 taaagcccaa tcttcggttt gtttgcgcgc atcaaggcaa cgtagcaacc gaacgcgcat
    19501 agcgacatcc tgctgatcaa gaatgcaggc ccagagtgtg tcttgaacat tgtattctct
    19561 acccggttta agacaaaccg ggatgatttg ttcgatcgga atggccagat cggttgcgat
    19621 gaattgcatg gctgctcgaa tattcatcgc tttggtgtca gtagggtcga ttaaattata
    19681 agggggttgc caaacgcttg cgggtcttaa taaatcgata tgactgacta caacgataat
    19741 cgaaggaggg cgatgattga gtcgtttgag ttgttcagca cgaagatgat ctaggaaacg
    19801 tcgctcatcc tggcgatccg gtcggtttgc cgcggttacc caaaagatca aatcggcatt
    19861 cagcgcagcg atgatcattt gttgctcatc aaacagttgg ctatcacaac cggggccatc
    19921 gaaaattagc gcttgctcca tcccttctcg ttctatagcg aaggaagaaa attgacgcgt
    19981 ggtgtcagga aggatatcgc ttgcagtggt taattttccg aataatgcgt tgatcagact
    20041 ggatttgccg gtattgcttc taccgagtac aagaaaacgc aaaggagggt gtgaggcttc
    20101 actgggatat cgggtttttt cttgttgtgg ctctgtggga ggagattcag tcggcacagt
    20161 tgccgtttta tcgataatcg gccggcgacc actatataaa tcgatcgcgt agtagcctac
    20221 tttgcgaata taagcgcgca aaaaccagcg ataaagctca tcgcgtgcca gactaaagct
    20281 acgttgcctg agatgttgcc agatttctcc gaacagcgca tgcatgggat tcaccaatgc
    20341 tctaccggcg cgatagacat tgatcgcctg ttcggcttta tctttccaac gttgaagtcg
    20401 caataaatca ccaatggtga agcgatcact gaaagggata ttttctgtga tatcgcgctg
    20461 taaatcgcgg ctcgcttgct caatgatcag taacgtatgc ggaacggtca attcgaggta
    20521 gggtttttct gcgtctgggt gatagcaatg ggagaccgtg tctagcgttt gtcgccctaa
    20581 ctccacgatc catgaagcat cttcaatgga ccaagcttga ggatcgcatt gattggcgag
    20641 ctgatcgact ttctgccagg caatttcagc gcttgggggc cagtcgggat tcggttccgt
    20701 gaccgcttcg cttaatagct gtcggtcgcg ccgaatcaat tgttgctgca gcagataacc
    20761 tgcgccacta catagaatca gtgcgatgag ccagtatacg aaaccatcga tttgccatag
    20821 ccatatcaaa ccgaataaaa ataacgccaa cgtagggcat agccacagaa tgagggctaa
    20881 taaccgaaag gggtcgaatt gtttgacgag ttttttcatg cgctaaattc gttgtttaag
    20941 aagggatgcg ctagcttcaa gcgcctcagc ataggctttt cgcagcaatt cagggttaat
    21001 gtttaagccg ttttgtctct gcgtaaagaa atacaccgct gctttaccca gcgcgtaggt
    21061 agtcgcggcg ctggcactgg ctccccaaac cgcacctacc gtttgtccga cgcctggaat
    21121 cagcttggtt agcgtgcgcc ctatcattct aacaaaatag ctcgaggtga ttgccgttcc
    21181 cagtaggcta aaaaattcaa tggtcgtttg tttattccat ttctgcccat acagctttgc
    21241 taaatcacga agtagcttgg cttgcagtgt cgtgaccatc accagatcga ctgctggcag
    21301 tgcgcctaat ccggcagccg ttagtgaata gcccacaata tgttgatgcg ccgttcgcgc
    21361 aaaaaaatcc tgaatttctt tttcaccggc aagttggcgc tgtaatccaa gcggtagcac
    21421 taactcaatc gccttccata gcggctccaa tccatagtgg gttggactga agccatcttc
    21481 cggtagcgtt agatcaacag gaacccaaag catgggtgca gaaccgggta gtgctatcgc
    21541 agtttgacgt tgtgcaacca aagcacgttg gagatcagta ggtacttcat tgggcaaagg
    21601 gtcctggtca aatggccatg gggtaagatg ttggtcgtgt ggcccgtaaa gttcatgtaa
    21661 acctgtttgt accaggatga tgggccaatc ggggtggcgt gttcgtaccg tctgaagcac
    21721 cttcaacacg atctgctgct gaagatcagc cactttcatc acggcaatga gaaggtgggc
    21781 ttgggattcg caatagtgaa tgtcttcgct ggggtcgtaa tcgatttcac ccaaaccgcg
    21841 cgtatccaga aaccgtacca cgggcatttg tgctgggaag tcataaaaac tcgagaagcg
    21901 cgtgcatggt tgaagaccat tgccgatttc agcggcttca ctgccagtga gggcatgaat
    21961 aattgatgtt tttccagatt gggttttacc gataagccaa aatacgggaa gcggtatttt
    22021 ttggcgtact tgccttaatg aagcgtctaa cgctgcctca tcgattttgg ggtcggccaa
    22081 aatggtttgc agtttttgcc aataagtgag ccagtcagtg attttgctta atttcacaat
    22141 tgcagtcttg aacgattaac gatgattatt gtacagaacc tcgggcgtgt gttagcaaag
    22201 ttagtctatt taggcagcaa agacatcacg aatcatgggt ggtagcgtaa atgggttaaa
    22261 tcgtcgggtg tgaataaatt cccaagccga tttaacgcca actgatatgg cggtgttata
    22321 ctatctgaca gtacggaatt cgcgggttga ttacgattg
//
LOCUS       DAOQLJ010000020        22033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_815_length_22033_cov_5.844208, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000020 DAOQLJ010000000
VERSION     DAOQLJ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 22033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22033
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_815_length_22033_cov_5.844208"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..373)
                     /locus_tag="PK522_02760"
     CDS             complement(29..373)
                     /locus_tag="PK522_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40687.1"
                     /translation="MKEDDPRQIIISKIIEHRENNTSQAEYSTSVSPVSRWLFYLALI
                     PVTIIVFFVGFFFFSILLAILLVAMAGIGARIWWLRRKFKQAANAQEEIKSAEIEDAE
                     IIETVIHKTNKK"
     gene            complement(360..1685)
                     /gene="pepP"
                     /locus_tag="PK522_02765"
     CDS             complement(360..1685)
                     /gene="pepP"
                     /locus_tag="PK522_02765"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HNC40688.1"
                     /translation="MSNKEQMQVYRERRNRLLDQLTHGIAVIATAPMRPRNRDSYYPY
                     RFDSYFYYLTGFEEPEAVLVVVAENQQGNAEQILFCRDKNLEREIWDGFRYGPDAACE
                     RFKFDSTYPISRLDEMMPKLLANQSTVYTMIGQDPAWDTQITGWVNRVREQTRSGITA
                     PDTLADVRKILDEMRLFKDEYELTLIRQAANIAVDAHRRAMKHANPGLFEYEIEAELI
                     HEFRRQGALAPAYTPIVAGGANACVLHYIENNMRLQSGDLLLIDAGCEYGGYASDITR
                     TFPINGRFTAVQRDLYQLVLSAQDAAIEAVQPGHTWDDPHQTALTVLVQGFIDLGLCQ
                     GSVEGIIESEDYKRFYMHRTGHWLGLDVHDAGEYKQAGAWRELKPGMLLTVEPGCYIR
                     PADNVPNHFWNNGIRIEDDVLVTTEGHEVLTAAAPKTIVEIEEWMYERR"
     gene            complement(1704..4448)
                     /gene="polA"
                     /locus_tag="PK522_02770"
     CDS             complement(1704..4448)
                     /gene="polA"
                     /locus_tag="PK522_02770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNC40689.1"
                     /translation="MKTLLLVDGSSYLYRAFHALPDLRNRHNKPTGAIYGVLNMLRRL
                     HKDFQSDYSACVFDAKGKTFRDEIYPEYKAHRPPMPSDLVDQITPLHDCIEAMGWPLL
                     IVEGVEADDVIGTLAKQACSHGMQCIISTGDKDIAQLVNHAIKLVNTMNNETLDAEGV
                     VKKFGVNPAQIIDYLALVGDSSDNIPGVEKVGPKTAVKWLSHYQSLDNLILHANEITG
                     VVGENLRKALPWLSVARQLLTIKCDVSLPVDFEGLITKQQDIPKLIALYDELEFKTWL
                     RELKTSNTLASGDREQTENQVTQHQNNSNSRANTKQSDIHYETITTETQLDRWIIQCE
                     QAPIVSVDTETTSLDPMQARIVGISFSIESSKAAYIPLGHTYTGVFDQLNLDYVLNKL
                     KPWLEDATKIKVGQNLKYDKHVLANHGVMLNGIAHDTLLQSYVLESHQPHNMDNLALR
                     HLDIETIHYDAVTGKGASKIGFEQVAIEQATRYAAEDADITLRLHRYLYPKIEQDRSL
                     QFVYHQIEMPLLAVLFQIERNGVLLDSNLLHAQSRDLAQQLYHLENQACALAGQVFNL
                     NSPKQIQEILFEQLKLPVIKKTPKGAPSTDEDVLQQLALDYPLPKVLLDYRGLAKLKS
                     TYTDKLPQMVNSNTGRVHTNYAQAVAVTGRLASNDPNLQNIPIRSPEGRKIREAFIAP
                     EGSQIISADYSQIELRIMAHISQDAGLLKAFAADEDIHQATAGEIFGVPIEQVNAEQR
                     RYAKVINFGLIYGMSEFGLASQLGIERSAARVYIERYFARYPGVAQYMQQTRERAREK
                     GFVETVFGRRLWLPEINSTHHNRRQGAERAAINAPMQGTAADIIKLAMIAVHNWLINH
                     QFKTKLIMQVHDELVLEVPDAEVAIVQANLSQLMENIGTFDVPLRVKVGIGKNWEQAH
                     "
     gene            4466..5185
                     /locus_tag="PK522_02775"
     CDS             4466..5185
                     /locus_tag="PK522_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNC40690.1"
                     /translation="MSLQEKLGGVMSLEKLWSTRESWRVFGIMSEFVEGTERLEHIQP
                     AVSIFGSARTDPSHPYYKLTEAIARQLSDAGFSVISGGGPGIMEAANKGAYFGKSPSI
                     GLNIQLPHEQHRNIYQDISQTFRHFFARKYMFVKFATAYVVMPVGFGTLDELMEALTL
                     VQTGKTRKMPIILVCSEFWNGLIDWFQKTLVQEGYIFPEDMHLIQIIDDPKQIVDAIF
                     QYYETSGFEPSPAEKELQLNL"
     gene            5216..5623
                     /locus_tag="PK522_02780"
     CDS             5216..5623
                     /locus_tag="PK522_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HNC40691.1"
                     /translation="MRPTEIRIGMYQIMTILLFSTIWFGTVSAQSNTANKSEPASPPP
                     PPDLINQSEQEMPELPDLGADPSMEPQVTIKKGDEETIEEYRVNGQLYMIKVIPRIGK
                     PYFLLNKRSPVGEIHRGDMESGVSVPMWQLYRF"
     gene            5698..6648
                     /locus_tag="PK522_02785"
     CDS             5698..6648
                     /locus_tag="PK522_02785"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNC40692.1"
                     /translation="MSVFTSVTQEQLSDWLQNYSLGTLIELQGITSGIENTNYRVITS
                     TGKYILTLFEKLTTTELPYYLNLMAHLSNQGIPCPNPVPTQTHRLLGEMNGKPASIVT
                     FLPGVSLTEPTIAHCAEIGTMLAKMHLAGLTYPAYMENPRGPKWWNIAAKAVMPFLSS
                     EDQKLLSTELEFQSAYRKINLPHGVIHADLFRDNVLFTETTVGGIIDFYFACNDALIY
                     DLAITLNDWCITEKIIFDDEKARALIRAYHCVRNLNSEEHMMWPIMLRAGALRFWLSR
                     LYDFHLPRPGELTHKKNPDHFRQILIDHQSRHTKLLEIWV"
     gene            6708..7529
                     /locus_tag="PK522_02790"
     CDS             6708..7529
                     /locus_tag="PK522_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HNC40693.1"
                     /translation="MEARHVRGRQGLQWILSGFYYFKLSPFVWMLLSSTFLMVELTLQ
                     ILPVLGIFAFLLISPVLVAGVMVGCQSLDQGQRLQLEHLFVGFRKNTAPLITVGGFNL
                     IALVLIVGIFMLMGGDALIDMLVYGKRFGENELMGIMDNVLSAWLAALGLSIPLMMAI
                     WFAPLLIIFENLPPAIAIRKSFFACLNNMAPFFVYGITLLILFFLISTATVKLLSLFG
                     AVPPPLILIILYIVILPTIFASIYASYQDIFPSEPPSEETNQNDEDTSSNSEANH"
     gene            complement(7532..9730)
                     /locus_tag="PK522_02795"
     CDS             complement(7532..9730)
                     /locus_tag="PK522_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNC40694.1"
                     /translation="MTSLLTGLNPEQAEAVTLPYQSALILAGAGSGKTRVLTTRIAYL
                     IQSGQASPNSLLAVTFTNKAAREMVTRLGALLPINTRGMWIGTFHGLCNRMLRAHYQE
                     ARLPQAFQIMDMGDQQSFIKRLMKALAIDEKAFPPRQIQWFINNAKEEGLRASQLEAH
                     DAFSRKMGEFYQIYEERCMQEGIVDFAELLLRCYELLLHHEALRHHYCDRFRYILIDE
                     FQDTNRLQYKWLKSLAGIGFPQHAAVFAVGDDDQSIYAFRGAHAGNMKEFERDFAVQK
                     IIKLEQNYRSHGNILDAANALIDNNQGRLGKNLWTAEGSGELIRVYNAATDFEEAAFI
                     VEEVKVLKAEGVPLSEIALLYRSNAQSRVLEHSLFNASLSYRVYGGMRFFERQEIKHA
                     LAYLRLVTQPEDDTSLLRIINFPARGIGSRSLEQLQDDAKQLGMSLWATALQKCGTAL
                     EQAKVQKGVTGFVALIKSMQQGCRDLPLPEIIRFVIAQSGLLAHYAADREGAERLDNL
                     NELINAAVSFVHESEDDSLISFLSHASLEGGEHQADQHQEALQLMTVHAAKGLEFHSV
                     FLSGLEEGLFPHENSRSEANGLEEERRLMYVATTRARRRLYLSFSQSRMLHGQTHYNV
                     PSRFIEEIPEHLLKWLQPKHQNLSNTDFNRGWRTTQNQLGGVSNLNHSVSSMSKQTTW
                     QIGRSVSHGTFGAGVIVNCEGQGKDLRVQVRFSRAGTKWLLLEYANLTLL"
     gene            complement(9887..10756)
                     /locus_tag="PK522_02800"
     CDS             complement(9887..10756)
                     /locus_tag="PK522_02800"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HNC40695.1"
                     /translation="MSQKHPVIAVTGSSGAGTSTVKNSFEHIFRREKLNVAIVEGDSF
                     HRYDRESMKAAVAESAKNNGRPISHFGPEANEFEKLEALFKEYGETGSGMTRLYLHND
                     EEAAPYNQKPGTFTPWKAIASGTDLLFYEGLHGGAKSDTADVAKYVDLLVGVVPIVNL
                     EWIQKIHRDMNARGYSAEAVTQTILHRMHDYVHYITPQFSRTHINFQRVPMVDTSNPF
                     IAREIPTLDESMVVIRFRDYKDVDFPFLLNMLHDSFMSRPNTLVVPGGKMGMAIELIL
                     TPLIIDLAKKSKH"
     gene            complement(11085..11882)
                     /locus_tag="PK522_02805"
     CDS             complement(11085..11882)
                     /locus_tag="PK522_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNC40696.1"
                     /translation="MRLISNLSRWVVWLCILLLSACATSPTGRTQLAFMPDEELDSMG
                     LQAFENLKKEKPIEQGGKNVQFVKCIAQAITREVGGEWEVVVFEDASLNAFALPGRKI
                     GVHTGLIDLVDNQDQLAAVIGHEIGHVLARHSNERMSQQVSTKLGLSIVQAVAAPTTP
                     LGQAAVSALGVGAQYGVIMPFSRLHESEADMIGIDLMAKAGFNPLESINLWKKMSHAS
                     QGNQPPEFLSTHPSHDTRIEDLQARLATAQPLYQQAMAAGKKPKCVK"
     gene            12005..14608
                     /gene="leuS"
                     /locus_tag="PK522_02810"
     CDS             12005..14608
                     /gene="leuS"
                     /locus_tag="PK522_02810"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNC40697.1"
                     /translation="MQEKYHPQEIEQDAQQYWEKTAAFNATELSGKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFHRMRGYNVLQPMGWDAFGLPAENAAMQHGLPPAKWTY
                     DNIAYMRKQLKSLGFSIDWTREIATCDPHYYRWNQWLFLRMLEQGIAYKKTQVVNWDP
                     IDQTVLANEQVIDGRGWRTGALIEKREIPGYYLAITRYADELLDTLDTLPGWPERVRT
                     MQANWIGKSYGVDITFPVSKPDEFSQDLKVFTTRADTLMGVTYLAIAAEHPLALHAAK
                     NNLSLAKFLEECKQGSTIEAELATQAKKGMDTGFTALHPITGESIPIWVANYVLMSYG
                     EGAVMAVPAHDERDFEFATQYQLPIKIVIRPQNEDELLPPNQAFIDYGITINSDEFSG
                     LQSQAAIDAIAAHLELKQLGKKRTRYRLRDWGISRQRYWGCPIPLIHCDRCGTVPVPD
                     DQLPVVLPENLVPDGSGNPLAKSSEFSVCSCPSCGQSARRETDTMDTFVDSSWYFIRY
                     ACADQNTCMSDSRASYWLPVDQYIGGIEHAILHLLYSRFWSKVMRDLGLVHFNEPFTN
                     LLTQGMVLNEIFFRKSATGRINYFNPTEIDIEVDAQGKRTKMMLRADTLPVESGGIGT
                     MSKSKNNGVDPQELVDSYGADTARLFMMFASPPTQTLEWSETGVEGAYRFLRRLWRQV
                     YLHLQHPSQPDAPVIDIKDWSPALKTLRGQLHQTIAKVTDDLERRHTFNTAIASIMEL
                     MNELAKLTDTESANRQLMQEALEKVVLLLSPIVPHICHTLWQELKPGTALLEQSWPEV
                     DHTALVQDTIDLVIQVNGKLRGQMNVPKDTDRATIEQLAINLENVQKIVAGNAIKKII
                     VVPGRLVNIVY"
     gene            14660..15166
                     /gene="lptE"
                     /locus_tag="PK522_02815"
     CDS             14660..15166
                     /gene="lptE"
                     /locus_tag="PK522_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNC40698.1"
                     /translation="MIVSRIFFIFSIVLALSACGFQLRGLVSNLPFETIHVTAPPGNP
                     IGIDVERAIRAGTDTKVVGSPENAQAILQIISTNNEKKILSLSAGGRVREFQLGYRVA
                     IRLVDPQGTEIAPIDEINLTRVLPFLDAQLLAKQAEEEMLYKDMQIDAVQQIIRRLAS
                     IKTTPKNN"
     gene            complement(15187..16011)
                     /locus_tag="PK522_02820"
     CDS             complement(15187..16011)
                     /locus_tag="PK522_02820"
                     /inference="COORDINATES: protein motif:HMM:NF018420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HNC40699.1"
                     /translation="MRKQRIASFFMLLILLVSYKQLFAIPVSLELALLVDVSRSVDAS
                     EYAQQKNGYVNAFKDPVIQDAIASFAAIGGIAVTYIEWSGANQQSVSVGWTQITNAAS
                     ANAFADAIDNVDRAFGLGGGTAPGSAIQFVTPKFSDNGFEGNRWVIDVSSDGIRNTGT
                     QASVARDHFLDLETVGEGLTKTINGLPIDNTDLGFLGLADWFQDNIVGGPNAFLIAAD
                     GGFDSFSDAVKTKIGREISEPPTSVVFEPTSLSLILIGLIGFYFVAYQPRRIRVQA"
     gene            16232..17275
                     /gene="holA"
                     /locus_tag="PK522_02825"
     CDS             16232..17275
                     /gene="holA"
                     /locus_tag="PK522_02825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNC40700.1"
                     /translation="MRIKPEQLPQLLKKQQNQCFTIMGDEPLLSIEAADTIRHSLYYQ
                     GVTEREIFTVDHRFEWSKITQWSDQCSLFSTSKLLDLRIPTGKVGNDGNTALVTFLDN
                     LSPDLWFMATLPTIDKKSQASKWFTALDNRGVIVAIDSIERTQLPQWIQQRFHAQKQT
                     IAPDALQLLVDNVEGNLLAAQQEILKLGLLYPEATLSVEQVRNAVFDVARHDVFDLPE
                     AMLTGDIVRFTHMLENLHSEGAAPPLVLSILVDPLRKLLLLHRGVSEGKSLGYLFKEI
                     RVWGQQQTLLSSAMKRYAPPLLMEGLTHAALIDKMIKGIKQGEIWDEFLQYGLRFAER
                     SQTKSFEQRILQT"
     gene            17303..17719
                     /locus_tag="PK522_02830"
     CDS             17303..17719
                     /locus_tag="PK522_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNC40701.1"
                     /translation="MKAKIKWQEGVSFSGETESGHTLIMDGAPEAGGRNLGPRPMEMI
                     LLGLGGCTAFDVVMILQKGRHPVTDCFIEIDAKRATEDPKVFTQIHLHFIVSGKNLAA
                     HHVERAIDLSAEKYCSASIMLRSTANITHDFEIIDV"
     gene            17824..18069
                     /locus_tag="PK522_02835"
     CDS             17824..18069
                     /locus_tag="PK522_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40702.1"
                     /translation="MNNDNNDDHKSDESLHENMRYFLFWMIEQQELLHQKNVKKAQCF
                     IALIRTLHFVKTQPCSSNTHKVSTQFIARKLNQLSCH"
     gene            18156..18929
                     /locus_tag="PK522_02840"
     CDS             18156..18929
                     /locus_tag="PK522_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HNC40703.1"
                     /translation="MEMITERIGNELAKFLSKPVRQSIQVAMISQEQLLATLQPADVL
                     LVEGNTRISTAIKYLTQSSWSHAALYIGNALPNDSPWNLPQVLVEADLKEGIRAVTIA
                     QYAQMHTRICRPIGLNDDDRKHLIDYAITRIGQQYDLKNIFDLARYLFPAPPIPTRFR
                     RRLLALGSGDPTRAICSTLIAQAFMSIHYPILPEVQRTWSDNPMHADCHAEIYHIRHH
                     SLYTPRDFDISPFFEIIKPTTRDHFDYHALTWDSETDNT"
     gene            complement(18977..19273)
                     /locus_tag="PK522_02845"
     CDS             complement(18977..19273)
                     /locus_tag="PK522_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40704.1"
                     /translation="MLFPDNLGVITCRCVLEAKKPVLFVSHAGGDWQMYCDDANHDFE
                     DEEAMRSELVVVHVAHLLAQDATLNDIADLPVDMGAERSQVGGSWVRFENADEE"
     gene            complement(19379..21118)
                     /locus_tag="PK522_02850"
     CDS             complement(19379..21118)
                     /locus_tag="PK522_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase family protein"
                     /protein_id="HNC40705.1"
                     /translation="MTYLYNQPDMFVEEMIQGFAAAHRDLIAAVNGGVVRRYQAEPGQ
                     VVVIIGGGSGHYPAFGGLVGPGLVHGAAMGNVFASPSAQQIYDVALAAYAGGGIILCY
                     ANYAGDVLNFNQAQARLQAQGIDVRAVVVTDDIASAPLAERQLRRGIAGALPVFKVAA
                     AAADSGESLDEVCRIANEANTRVRTLGVAFSGCTLPGAQQPLFHVEHGKLAVGMGIHG
                     EPGLYQQDGLPADDLAAMLVEKLLQDISPVGDLPKGTHISVLLSSLGSVAQEELFVVY
                     RKVDQLLHSRGLIIVEPVVDRVITSFDMAGISLTLFWLNDELETYWKAAADTPAFKRG
                     TISCFRPETDAALIPQVAVASQSVTVGSPASQRIAAIILEIFASAKQIIETQQEELGR
                     LDAVAGDGDHGIGMLRGVSAALDAAKKAHDAQAGAGTLLTLSGEAWAARAGGTSGALW
                     GVILNTFGVKLGDTTAPDTATVAGSVAAALCEVMQRGKAQPGDKTLVDVLQPFSAALD
                     QASARGISVVTAWQDAADIANTAAQQTRNLLPRIGRARPHAEKSIGTPDPGAISLAMI
                     ITVASEVIRKHCP"
     gene            21665..>22033
                     /locus_tag="PK522_02855"
     CDS             21665..>22033
                     /locus_tag="PK522_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HNC40706.1"
                     /translation="MTTSQTLPQKITKSYVDKLSVPETGQAFIRDTELKGFAVRITSS
                     GAKSFILEKRINGKVKRLTIGRYPEITVEQARKEAHKLLGHIATGRNPIAEKKQEALQ
                     GTTLKQAFDDFVKTRKNLKER"
BASE COUNT         6348 a         5229 c         4308 g         6148 t
ORIGIN      
        1 accaaactaa tattcacggg attactaact actttttgtt cgttttgtga attaccgttt
       61 caattatttc agcatcttcg atctctgcag acttgatctc ttcttgcgca ttcgctgctt
      121 gtttgaattt acgtcgcaac caccaaatcc ttgcaccaat ccccgccata gctaccaata
      181 gaatagctaa taaaatcgag aaaaagaaaa agcccacgaa aaaaacgata attgtgactg
      241 gaataagcgc caagtaaaat aaccaacgac taacaggaga taccgaagtt gaatattcag
      301 cctgagatgt attattctct cgatgttcga tgatcttgct aataatgatt tgtctagggt
      361 catcttcttt catacatcca ctcctctatt tccacgattg ttttgggcgc agcagcagtc
      421 aaaacttcat gtccctcggt ggtaaccaac acatcatctt caatccgaat gccgttattc
      481 caaaaatgat taggaacatt gtcagcagga cggatatagc acccaggttc aaccgttaac
      541 aacatgcctg gttttaactc gcgccatgca ccagcttgct tatattcacc cgcatcgtgc
      601 acatccaaac caagccaatg tcccgtacga tgcatataga aacgcttata atcttccgat
      661 tcaataatgc cttcaacact accttgacac aacccaagat caatgaatcc ttgaacaagg
      721 actgtcaatg ctgtttgatg gggatcatcc caagtgtgtc cgggttgcac agcctcaata
      781 gccgcatctt gtgcagaaag aaccaattga taaagatcgc gttgtactgc ggtaaatcgc
      841 ccatttatcg gaaaagttcg tgtaatatcc gatgcatacc cgccatattc gcaacccgca
      901 tcgatcagta ataaatcacc cgattgaaga cgcatgttat tttcaatgta atgaagtaca
      961 caagcgtttg cacctcccgc tacaattggg gtatatgctg gtgctaatgc gccttggcga
     1021 cgaaactcat gaattaattc tgcttcaatt tcatattcga ataaaccggg attagcatgc
     1081 ttcattgcac gccgatgggc atctactgca atgttagcgg cttgccgaat tagggtaagt
     1141 tcatactcat ctttaaacaa gcgcatttca tccaagattt tgcgcacatc ggcaagcgta
     1201 tccggtgcag tgatgccgct tcgggtttgt tcacgaacac gattaaccca tcctgtgatt
     1261 tgcgtatccc aagctggatc ttgtccaatc atcgtataga cagttgattg gttcgcgagt
     1321 aacttaggca tcatttcgtc gagtctgctg atcgggtagg tgctgtcaaa tttaaaccgc
     1381 tcgcatgcag catcgggacc ataacgaaaa ccatcccaaa tctctcgctc taaattctta
     1441 tcacgacaaa ataaaatctg ctctgcgtta ccttgttgat tctctgcaac cacaaccaat
     1501 accgcttctg gttcctcaaa accagtcaaa taataaaaat aactgtcaaa ccgatatgga
     1561 taatagctat ctcggtttct gggtcgcatc ggtgctgttg caatcacagc aataccatgg
     1621 gtcagttgat caagtaaacg attacggcgc tcacgataaa cttgcatttg ttctttatta
     1681 ctcatttttt catatcaaaa agatcaatgg gcctgctccc aattttttcc gatcccgacc
     1741 ttgactcgta agggaacatc aaatgtacca atattttcca tcaattgcga taagtttgct
     1801 tgaacgattg ccacctcagc atcaggcact tcaagcacta attcatcatg aacctgcatg
     1861 atgagcttgg tcttaaattg atgattgatc aaccaattat ggactgcgat catcgccagt
     1921 ttaatgatat cagccgcggt cccttgcatg ggagcattga tcgccgcacg ctcagcgcct
     1981 tgtcgacgat tatgatgcgt actattaatt tccggtaacc acaaacggcg accaaatacg
     2041 gtttcaacaa aacccttttc tctcgctcgc tctcgcgtct gctgcatata ctgagcaacg
     2101 cctggataac gtgcaaaata gcgttcgata taaacacggg cggcactacg ttcgatcccg
     2161 agttggctgg ccaaaccaaa ttctgacatc ccatagatta aaccaaaatt aattactttt
     2221 gcgtagcgcc gttgctcggc attcacctgc tcaatcggta caccaaaaat ttctcccgca
     2281 gtcgcttggt gaatatcctc atctgcagca aatgctttca gtagccccgc atcttgtgag
     2341 atatgcgcca taattcgcaa ctcgatctga gaataatcgg cagaaatgat ttgactcccc
     2401 tctggcgcaa taaatgcctc ccgtattttg cgtccttctg gacttcgaat gggaatgttt
     2461 tgcaaattgg gatcattgct tgccaatcgc cctgtgactg caactgcctg ggcataattg
     2521 gtatggacac gtcctgtatt ggaattgacc atttgaggca atttatcggt atacgttgac
     2581 ttaagcttgg ccagccctct gtaatccaac aatactttgg gcaaaggata atccaatgca
     2641 agctgctgca aaacatcttc atcggtagaa ggggctccct ttggcgtctt tttaatgacg
     2701 ggtaacttca gttgttcaaa tagaatctct tgaatttgct tgggagagtt tagattgaat
     2761 acctgaccag ccagggcaca agcctgattt tccaaatgat aaagctgttg cgcaagatcc
     2821 cggctttgcg catgaagtaa gttagagtcc agtaatacgc catttcgttc gatttgaaaa
     2881 agtacagcga gcaatggcat ttcaatttgg tgatacacga actgtaatga acgatcttgc
     2941 tcaattttag gatagagata gcggtgcaac cgcaaggtaa tatctgcatc ttctgctgca
     3001 tatcgtgttg cctgctcaat cgccacttgc tcaaatccga tcttactcgc accctttcct
     3061 gttaccgcat catagtgaat cgtttcaata tcgagatggc gcaatgccag attatccata
     3121 ttatggggtt gatgtgattc cagcacatag gattgcaata acgtatcatg cgcaatccca
     3181 ttgagcatta cgccgtgatt agccagaaca tgtttgtcgt attttaaatt ttgacctact
     3241 ttgatttttg ttgcgtcttc cagccagggt ttcagtttgt ttagtacgta atctaaattt
     3301 aactgatcga atacccctgt ataagtatgt cctaatggaa tataggctgc tttactagat
     3361 tcaatactga atgagatacc aacgattcgc gcctgcatcg gatctaagct cgtcgtttcc
     3421 gtatcgactg aaacaatggg cgcctgctca cattgtatta tccatcgatc aagctgggtt
     3481 tctgtggtta tcgtttcata atgaatatcg ctctgtttag tattcgctcg tgaattcgag
     3541 ttgttttggt gttgtgtaac ttgattctcc gtttgctccc tatcccctga agcaagggta
     3601 ttactcgtct tgagttcacg tagccaagtt ttaaactcta actcatcata gagcgcaatc
     3661 aacttgggaa tatcctgctg cttagtaatg agtccctcaa aatcaaccgg tagcgataca
     3721 tcgcatttaa tggtcagtaa ttgacgggct acacttaacc atggcaatgc tttacgcaaa
     3781 ttctccccaa cgacccccgt aatctcattg gcgtgtaaga ttaaattatc cagagattga
     3841 tagtgtgata accacttgac tgcagtcttt gggcccactt tctcaacacc aggaatattg
     3901 tcagaactgt cacccaccaa tgctaaataa tcaatgatct gtgctggatt taccccaaac
     3961 tttttaacga ccccttctgc atccaacgtc tcattattca tggtattaac cagctttatt
     4021 gcatgattaa cgagttgcgc aatgtcttta tcgcccgttg aaataatgca ttgcatccca
     4081 tgactgcaag cttgtttagc caatgttccg atcacgtcat ctgcttcaac accctccaca
     4141 atcagcaaag gccagcccat cgcttcgata caatcatgca agggcgtaat ctggtccact
     4201 agatcactcg gcatgggggg acgatgcgct ttatattcag gatagatctc gtcgcgaaat
     4261 gttttgcctt ttgcatcaaa cacgcaagcg ctataatctg actgaaaatc cttatgtaag
     4321 cggcgtaaca tattcagcac cccataaatg gcacccgttg gcttattatg acgattacgc
     4381 aaatcaggca atgcatgaaa agcacggtac aagtaagacg agccatcaac taacaagagt
     4441 gttttcatct aaagaaagga cctatatgag cttgcaagaa aaattaggtg gcgtcatgtc
     4501 attggagaaa ctttggtcaa ctcgggaatc ctggcgtgtg ttcggtatca tgtccgaatt
     4561 cgtcgaagga accgaacgct tagaacatat tcaaccggca gtcagtatct ttggcagtgc
     4621 acgcacggat ccaagccacc cctattataa attaaccgaa gcgattgcac gacaattgtc
     4681 ggatgccggc ttttctgtca tctcaggagg tggtcccgga attatggaag ctgccaacaa
     4741 gggggcttat tttgggaaat cgccaagtat tgggctcaat attcaactac cacacgagca
     4801 gcatcgcaat atctaccaag atattagcca aacctttcgc catttctttg ctcgcaaata
     4861 tatgttcgta aaatttgcca cagcttatgt ggtaatgcca gtggggtttg gtacgctcga
     4921 tgagttaatg gaagcattaa cgctcgttca aacaggaaaa acaagaaaaa tgccgattat
     4981 cttggtctgt tcggaattct ggaatggtct aatcgattgg tttcaaaaaa cgctggtcca
     5041 ggaaggttac atttttcctg aagatatgca tttaatacaa attattgatg accctaaaca
     5101 aatcgtcgat gccatctttc aatactatga aacctctggt tttgaacctt cccccgctga
     5161 aaaagaatta cagctaaatt tataaatcat ttatttttcg gatagaatgg ctgctatgcg
     5221 ccctacagaa atcaggatcg gtatgtatca aattatgact attttattat tcagtacaat
     5281 ctggtttgga acggtatctg ctcagtccaa tactgcaaat aaatctgaac ccgcttcccc
     5341 tccacccccc cctgatttaa ttaaccagtc tgaacaagaa atgcccgaat tgcctgatct
     5401 tggcgctgat ccctcaatgg aaccgcaagt caccattaaa aagggggatg aagaaacgat
     5461 tgaggaatat cgggttaacg gccagcttta tatgattaaa gtcattcctc gtatcggtaa
     5521 gccctatttt ctgctcaata aacgcagccc tgttggagaa attcaccgcg gtgatatgga
     5581 atcgggtgtt agtgttccaa tgtggcaact ttatcgattt taaatttaac ccaacaatta
     5641 taggtaatga ttcagttggg ttgaataacg tttttcagtt tctctttact ttatttcatg
     5701 tcggttttta catcggttac ccaggaacaa ctttctgatt ggcttcagaa ctattcgcta
     5761 ggaacgctta ttgaacttca agggattact tccggtatcg aaaataccaa ttatcgggta
     5821 ataacttcta ccgggaagta cattcttaca ttatttgaga aattaacaac tactgaacta
     5881 ccgtattacc ttaatttgat ggcccattta tcaaatcaag gtattccctg ccctaatccg
     5941 gttccaaccc aaacacaccg attactgggc gaaatgaatg gaaaaccagc cagtatcgta
     6001 acttttctac ctggcgtatc gttgactgaa ccaaccattg cgcactgcgc tgaaatcggt
     6061 accatgcttg caaaaatgca tctagctgga ttaacctacc ctgcctatat ggaaaaccca
     6121 cgggggccca aatggtggaa catagcagct aaagcggtaa tgcctttctt atcctcagaa
     6181 gatcagaaac tgctaagtac agagcttgaa ttccaatctg cttatcgaaa gatcaatctt
     6241 ccccatggag tcattcatgc agatctgttc cgcgacaacg tattgtttac ggaaacgacc
     6301 gttggtggca tcattgattt ctattttgct tgtaatgatg cgttaattta cgatttagcg
     6361 attacactca atgattggtg tataactgag aaaattattt ttgatgacga aaaagcacgt
     6421 gcgctaattc gcgcttatca ctgcgttcgt aacttgaatt ctgaagaaca catgatgtgg
     6481 cccatcatgc tcagagcggg tgcattaaga ttctggttat cgcgcttata cgattttcat
     6541 ttgcctcgcc ctggagaatt aacgcacaag aaaaaccccg atcatttccg acaaatattg
     6601 atagatcatc aatcacgtca tacgaaactt ttggaaattt gggtgtagct ttttacgcag
     6661 tatcagtatc actatccttt catctatcaa ttggagaaaa agaattcatg gaagctcggc
     6721 atgttcgtgg gagacaaggt ttacagtgga tcttaagtgg tttttactat tttaagttat
     6781 ctccgtttgt ttggatgcta ctcagctcca catttctgat ggtcgaactc accttgcaaa
     6841 tcctccctgt tttaggaatc tttgcctttt tattgatttc acccgtccta gttgctggcg
     6901 taatggttgg ctgccaaagt cttgatcaag gccagcgact tcagcttgaa cacttattcg
     6961 ttggttttag gaaaaatacc gctccactga tcacagttgg cggatttaat ctaatcgcgc
     7021 tcgttctcat tgtcggtatt tttatgctaa tgggtggtga tgccctcatc gatatgctcg
     7081 tttatggaaa acgatttggc gaaaatgaat tgatgggaat catggataac gtattatcag
     7141 cttggctcgc tgctctaggg ctaagtattc ctttgatgat ggcgatatgg tttgcgccct
     7201 tgctcattat ttttgaaaac ctaccccctg cgattgcaat acgaaaaagt ttctttgcct
     7261 gtctaaacaa tatggcgcct ttctttgtct atggcatcac actattgata ttgtttttcc
     7321 tgatttctac cgccacagta aaactattga gcctctttgg tgccgtacca ccgccactga
     7381 tactcattat tctctatatc gtaatactac caacgatctt tgcttccatt tacgcaagct
     7441 atcaggatat ttttccctct gaaccaccct cagaagaaac taaccagaac gacgaagata
     7501 cgagtagcaa ctcagaagcc aatcattaat atcagagtag cgtcaagttg gcgtattcaa
     7561 gcaggagcca cttggttcct gcacgactaa atcgaacttg aactcgtaga tcttttccct
     7621 gaccttcaca gttaacgatg acaccagcac caaaagttcc atgagaaacg gagcgcccaa
     7681 tctgccaggt agtttgttta ctcatactac taactgaatg attaagatta gatacaccac
     7741 ccaattgatt ttgagttgtt ctccacccac gattaaaatc tgtgttcgat aaattctgat
     7801 gcttaggttg taaccatttt aataagtgct ccgggatttc ttcaatgaac cgtgaaggta
     7861 cattgtaatg tgtctgacca tgaagcatac gactctgaga aaaactcaaa tacagccgcc
     7921 ggcgtgcacg cgttgttgcc acatacatca aacggcgttc ttcttcgaga ccatttgcct
     7981 ctgatcgact gttttcatgt ggaaagagcc cctcctccaa accactcaaa aacacactat
     8041 gaaactctaa tcctttcgct gcatgaactg tcatgagctg taatgcttct tgatgttgat
     8101 ccgcttgatg ctcaccaccc tctagtgacg catgtgacaa aaatgaaata aggctatcat
     8161 cttccgattc atgtacaaaa cttaccgcag cgttaatcag ctcgttcaaa ttatcaagcc
     8221 gctcagcacc ttcacgatca gccgcataat gcgctaataa gccactttgt gcgatcacaa
     8281 aacgaatgat ttctggtaaa ggtaagtcac gacacccctg ctgcatcgat ttaatcaacg
     8341 caacaaaccc tgttactccc ttttgaactt tagcctgttc gagcgccgtt ccacattttt
     8401 gcagagcagt cgcccataaa ctcattccga gctgtttcgc atcatcctgt aattgttcaa
     8461 gactacgtga tccaatccca cgcgcaggaa aattaatgat gcgtaacaat gaggtatcat
     8521 cttctggttg agtcactaat cggaggtaag ccagggcatg tttgatttct tgccgctcaa
     8581 aaaatcgcat accaccatag actcgatacg ataacgaagc attaaacaag ctgtgctcca
     8641 acacccttga ttgcgcattc gatcgataaa gtaaggcgat ttctgagagc ggtacacctt
     8701 cagcttttaa cacttttacc tcttctacaa tgaaagccgc ttcctcaaaa tcggtagccg
     8761 cgttatagac tcgaattaac tccccactgc cttccgccgt ccataaattc ttacctaaac
     8821 gcccttgatt attatcaatc agcgcattgg ctgcatcgag gatattgcca tgagaccggt
     8881 agttctgctc aagtttgatg attttctgaa cagcaaaatc gcgctcaaat tctttcatat
     8941 ttccagcatg cgcaccgcga aatgcataaa tactttgatc atcatcaccc accgcaaaaa
     9001 cggctgcatg ctgaggaaag ccaattccag ccaatgactt gagccattta tactgcaatc
     9061 gattagtatc ttgaaattcg tcgatcagaa tgtaacggaa tcgatcgcaa taatggtggc
     9121 gcaatgcttc atgatgaagc agcagttcat agcaacgcag caggagttca gcaaaatcaa
     9181 cgatgccttc ttgcatacat cgttcttcgt aaatttgata aaactctccc atcttacggg
     9241 aaaaagcatc atgcgcttct aactgtgaag cgcgcaaacc ctcttctttt gcattattga
     9301 tgaaccactg aatttgccgc ggtggaaatg ctttttcatc gatggcaagc gctttcatca
     9361 gccgcttgat gaacgactgt tgatctccca tatccatgat ctgaaatgct tgaggtaacc
     9421 gggcttcctg gtaatgggcg cgcaacatac gattacataa gccgtgaaaa gtaccaatcc
     9481 acataccacg cgtattaatt ggcaataaag cccctaaccg tgtcaccatt tctcgtgcag
     9541 ctttattggt aaaagttacc gcaagtaagc tattgggact tgcctgacct gattgaatca
     9601 aatacgcaat ccgcgtggtc agtacccgcg ttttaccgct tcccgccccc gcgagaatta
     9661 aagcagattg ataaggtaat gtaacagctt ctgcctgctc gggattcaat ccagtaagta
     9721 aagaagtcat gagagaatgt gtccagcacg tttggtttgc taggattgag taatcattcc
     9781 tagttaatcg gggttaaatc ttcttcagaa acaaaagaac cttaccaact cgttgaaatt
     9841 cggtaaggtt cagttagatt atcagcgtgt tacaactaac gcttcattaa tgcttgctct
     9901 tttttgctaa atcgataata agtggcgtta aaatcaattc aatggccatg cccattttac
     9961 cgcccggaac aacaagggta ttgggacgag acatgaaaga atcgtgcaac atatttaata
    10021 agaatggaaa atcaacatcc ttgtaatcac ggaatcgaat caccaccata ctttcatcga
    10081 gcgttggtat ctctctagca atgaatggat tcgaagtatc caccatggga acgcgttgaa
    10141 aattaatatg ggttcttgaa aattgcgggg ttatataatg cacataatca tgcatacgat
    10201 gaagaatcgt ttgtgttaca gcctctgctg aataaccacg tgcattcata tcacgatgaa
    10261 ttttttgtat ccattccaga tttacgattg gtaccacacc aaccaataaa tcaacatatt
    10321 ttgcaacatc tgcagtatcg ctttttgctc ccccatgaag gccttcataa aaaagcaaat
    10381 cggtacccga tgcaatcgct ttccaagggg taaaggttcc tggtttttga ttatatggcg
    10441 ctgcttcttc atcattatga aggtacaaac gcgtcattcc agaacctgtt tccccatatt
    10501 ctttgaacaa ggcttccaat ttctcaaatt cattggcttc tgggccaaaa tgactaatcg
    10561 gccgcccatt gttttttgct gactctgcaa cggcagcttt cattgattct cgatcataac
    10621 gatggaagct atccccttca acaatcgcta cattgagttt ttcacgacga aaaatatgtt
    10681 caaaactatt tttgacagtc gatgtaccgg cgcccgaaga tccagtcacg gcaataacag
    10741 gatgtttttg tgacatttgc tactttctcc ctataaatac actaataaaa aataatgaat
    10801 agtaagattt tttttgttat aactttaaca gcgtatgata cctgctttcg agcagatttg
    10861 ggcaatcaaa aattgaaagg cagaatcaaa gaattataaa ttattcttat atatcaatta
    10921 tttatccaat ataaggtcat gaataaaaat acccgctttg tagaataagc ttctaaaata
    10981 atcttggtca actctgctgc attcaccata ccatcatcgc cctaatcatt cttattactg
    11041 ccactgattt gaacaacttt cttatagtct tagaatccaa ctgattactt tacacacttc
    11101 ggttttttac ctgcggccat cgcttgctga taaagcggct gtgctgttgc aagccgcgct
    11161 tgcaaatcct caatacgggt atcatgcgaa ggatgcgttg ataaaaactc cggcggctga
    11221 ttcccttgac tggcgtgact catttttttc cacaaattga tgctttctag cgggttgaat
    11281 cccgcttttg ccatcagatc gatgccgatc atatccgcct cgctttcgtg caagcgacta
    11341 aatggcatga ttaccccata ctgggcacca acccctaatg cacttaccgc agcttgcccc
    11401 aatggtgtcg ttggtgccgc tactgcctgt acaatcgata atccaagctt tgtactgacc
    11461 tgttgcgaca tgcgctcatt actatgccgc gcgagcacat gcccaatttc atgcccaatg
    11521 accgctgcaa gttgatcttg attgtctact agatcgatta atccggtatg aacgccaatt
    11581 tttcttcccg gcaatgcaaa ggcattcaac gaagcatctt cgaaaaccac tacttcccat
    11641 tcaccaccta cttctcgggt aattgcttga gcaatacact tcacaaactg aacgtttttc
    11701 ccaccctgct cgattggttt ttcttttttt aaattctcga atgcctgcaa acccatgcta
    11761 tctaattctt catcaggcat aaaagctaat tgcgttcttc cagttggtga cgtcgcgcag
    11821 gcactcaata acagaataca cagccaaaca acccatcggc ttaaattact aatcaatctc
    11881 atttgtagct cctttacgaa ttaatttcac atttaaaatg aggctaccat gatgcgttgg
    11941 ttcgcatcaa gcgaagcagt tcaagttata attcgatttt cgattaaacc aggataaatg
    12001 ttgcatgcaa gaaaaatatc acccacaaga aatcgagcag gatgcacagc aatattggga
    12061 aaaaacggct gctttcaatg ccacagagct ttcaggtaaa cctaaatatt attgcttatc
    12121 catgtttccg tacccgtctg gtaaacttca tatggggcat gttcgaaact atacaattgg
    12181 ggatgtgcta tcacgtttcc atcgcatgcg cggttataac gttttacaac cgatgggttg
    12241 ggatgcattt ggtttgccag cagaaaatgc tgcaatgcaa catggtctac caccggcaaa
    12301 atggacttat gacaatattg cttatatgcg caagcaactt aaaagtcttg gtttcagtat
    12361 cgattggacg cgtgaaattg ctacctgtga tccccattat tatcgctgga atcaatggct
    12421 ttttttacgc atgctcgaac aaggcattgc ctacaaaaag acccaagtag tcaactggga
    12481 tcccatagac caaacggtac tcgctaacga gcaagtcatc gatggccgtg gctggagaac
    12541 cggtgcactc atcgaaaaac gtgaaatccc gggttattac ctcgcaatta cccgttacgc
    12601 tgacgaatta ttagacacgc tcgatacgct accgggctgg ccagaacgtg tcagaacgat
    12661 gcaagccaac tggattggta aaagttatgg tgtagatatc acttttccag ttagcaaacc
    12721 agatgaattt tcccaggatt tgaaggtttt cacgacacgc gcagatacgc tcatgggtgt
    12781 tacctatttg gcgattgcag cagagcaccc cctcgcatta cacgcagcta aaaataatct
    12841 tagccttgcc aaattccttg aagaatgcaa acaaggctca accatcgaag cagaattggc
    12901 tactcaagcg aagaaaggca tggatacagg cttcaccgcg ctccatccga ttacggggga
    12961 aagtattccc atctgggtcg caaactatgt gctaatgagc tatggagaag gtgctgtgat
    13021 ggcagtacct gcacatgatg aacgcgactt cgaatttgca acacaatatc aacttcccat
    13081 caagatcgtt attcgacccc aaaatgagga tgaattgctt ccacccaatc aagcatttat
    13141 tgattatggt attaccatca attcagacga attttctggg ttgcaatctc aagctgcaat
    13201 tgatgcaatc gcagctcatc tagaactcaa acaactcggt aaaaaacgga cacgttaccg
    13261 cctacgcgat tggggaattt cacggcaacg ttattggggc tgccctattc ccctcatcca
    13321 ctgcgatcgc tgtggtaccg ttccagttcc tgatgatcaa cttccagttg tactgccaga
    13381 aaatctagtt cccgatgggt caggcaaccc actcgctaaa tcaagcgaat ttagtgtctg
    13441 ctcgtgcccg tcctgtggac agtctgctcg gcgggaaaca gacacaatgg atacctttgt
    13501 cgactcatct tggtatttca tacgctatgc atgcgccgat cagaatacct gtatgtcgga
    13561 ttcgcgagcc agctattggt tgcctgtcga tcagtatatt gggggcattg agcacgccat
    13621 tctgcatttg ctctactcac gattctggag caaagtcatg cgagatcttg gattagttca
    13681 cttcaatgaa ccgtttacta atctattaac tcaaggaatg gtattaaatg aaatcttctt
    13741 ccgcaaatca gcaacgggac gcatcaatta tttcaacccg actgagattg atatagaagt
    13801 cgacgcgcaa ggtaaacgaa ccaaaatgat gttacgtgcc gatactctac cagtcgagtc
    13861 cggtggaatc ggtaccatgt cgaaatcgaa aaacaatggc gttgatcccc aagaactagt
    13921 cgattcctat ggtgcggata cggctcgact gttcatgatg tttgcgagtc ccccgacaca
    13981 aacactggaa tggtcagaaa caggcgtaga aggggcttat cgttttctca ggcgattatg
    14041 gcgacaggtt tatctccatc tacagcatcc ctcccaaccc gatgcgccgg ttatcgatat
    14101 caaagattgg tcaccagccc tcaaaacgct tcgcgggcaa ttgcatcaaa ctatagctaa
    14161 agtcaccgat gatctggaac ggcggcatac gttcaatacc gccattgcgt ccattatgga
    14221 attaatgaat gaattagcga aactcactga tactgagtca gcgaatcgac aattgatgca
    14281 agaagcgttg gaaaaagtgg ttctattgct atcacctatt gttcctcaca tctgccatac
    14341 attatggcag gaactcaaac ctggtacagc gttactcgag caaagttggc ctgaggtcga
    14401 tcataccgca ttagttcaag ataccattga tctggtaatc caagtcaatg gaaaattacg
    14461 gggacagatg aatgttccta aagataccga tcgtgctacg attgaacaat tagcaattaa
    14521 tcttgagaat gtgcaaaaaa tagttgcggg aaatgccatc aaaaagataa ttgtcgtacc
    14581 tggaagactc gttaatatcg tatattagtc gctcgatgta gcctgattta cttaaaattt
    14641 tgaaagaagg tacaaccgca tgatcgtttc tcgtatattt tttatcttca gcatagtact
    14701 cgcgctctca gcctgtggat ttcagttgcg gggacttgta tccaatcttc cctttgaaac
    14761 gattcatgtg acagcgcccc caggtaaccc gattggaatc gatgtggaac gcgcgatccg
    14821 tgctggaacc gataccaaag ttgttggatc ccccgaaaat gcgcaagcta tccttcagat
    14881 catcagcacc aataatgaaa aaaagatact ttcactttct gcgggaggga gggtaagaga
    14941 attccagctt ggctatcgcg ttgctattcg gctggttgat ccacaaggta ctgaaattgc
    15001 cccaatcgat gaaattaact tgacacgcgt actgcctttt ctcgatgcac aattacttgc
    15061 gaaacaagct gaggaagaga tgctttataa agatatgcaa atcgatgcag tgcagcaaat
    15121 catccgccga cttgcttcta tcaaaactac acctaaaaat aactagcgca ttctcttcct
    15181 ggttaattag gcctgaactc gtattcttct cggttgataa gctacaaaat agaagccaat
    15241 taaaccaatc agaatcaagg acaggctcgt aggctcgaaa acgacactcg tcggcggttc
    15301 actgatttcc ctcccaattt ttgtctttac tgcatcgctg aagctatcaa accctccatc
    15361 cgcagcaatc aagaacgcat tagggccacc cacaatatta tcctggaacc aatccgccaa
    15421 gcctaagaaa cccaagtctg tgttatcaat gggcaaacca tttatcgttt tagttaaccc
    15481 ttcccctact gtttctagat caagaaagtg atcacgggct accgaagctt gcgttcccgt
    15541 atttcggata ccatcactgg atacatcgat tacccaacga ttcccttcaa aaccattatc
    15601 actaaattta ggcgtaacaa actggattgc gctgcctgga gctgtccctc cgcctaaccc
    15661 aaaagcacga tcgacattat cgatcgcatc agcaaatgca ttggcgcttg ccgcgttagt
    15721 aatttgtgtc caaccgacgc taaccgattg ctgattggca ccagaccatt cgatataagt
    15781 caccgcaatg ccacctatcg ctgcaaatga agcaatcgca tcctgaatga ccggatcttt
    15841 gaatgcgtta acatagccat ttttttgttg cgcatactcc gatgcatcaa cacttcgcga
    15901 tacatccacc aacaaagcca actccaagct aacaggtata gcaaacagtt gcttataact
    15961 aactaatagt attagtagca taaaaaaact ggcaattctt tgctttctca tcatgtctct
    16021 ccatacttaa aatataaaat caatcccctg gtgatcgtga agtaacgcaa aatctaggcc
    16081 aaccattgta attcattgat atagaatgga atcttagaat agaacgtcat tttggcttta
    16141 agtttgtaaa tatccttgac aaaaatacta acgactgtac caataacttc atttcattta
    16201 aatttagact taattcttcc tgtattgata tatgcgaatt aaacccgagc aactccctca
    16261 gttattgaaa aaacaacaaa accagtgttt tacgatcatg ggggatgaac ctttattatc
    16321 gatcgaagcg gcggatacca ttcgccattc cctttattat caaggtgtga ctgagcgaga
    16381 aattttcaca gtagatcatc gcttcgaatg gagcaaaatc acgcaatgga gtgatcaatg
    16441 ttctttgttt agcacctcta aattgcttga tctccgaatt ccgacaggca aagtaggcaa
    16501 tgatggcaac actgcacttg taacttttct tgataatctc tcgcctgatt tatggtttat
    16561 ggcaacgtta ccgacgattg ataaaaaaag ccaagcaagt aaatggttta ctgcattaga
    16621 taaccgcggt gtgatcgtag caatcgattc aatcgaacga acccagcttc cacaatggat
    16681 tcagcagcga ttccatgcgc aaaagcaaac catagcaccc gatgcattac aattgttagt
    16741 cgataacgta gaaggaaatt tactggccgc acaacaggaa atcctcaagc ttggtctcct
    16801 ttatcccgaa gcaacgcttt ctgttgagca agtcagaaat gctgtgtttg acgtcgcacg
    16861 acatgatgta ttcgatttac ctgaagccat gctcactggg gatattgtgc gctttacgca
    16921 tatgctggaa aatctacaca gtgaaggtgc tgctcccccc ctggtcttga gtatcctcgt
    16981 cgacccactc cgaaaactat tgttactcca ccgcggtgtt agtgaaggaa aatcgctagg
    17041 gtacttattc aaggaaattc gagtctgggg tcaacaacag acattattat cttcagcgat
    17101 gaagcgctat gccccccctt tattaatgga ggggctgacg catgctgcgc tgatcgataa
    17161 aatgatcaaa gggattaaac aaggtgaaat ctgggatgaa ttcctacaat atggactgcg
    17221 ttttgccgag cgcagccaaa caaaatcatt tgaacagcgt atactgcaaa cttaacaaga
    17281 atcaatcaat tttagaaaaa taatgaaagc taaaatcaaa tggcaagaag gtgttagttt
    17341 ctctggagaa accgaaagtg gacatacgct gatcatggat ggcgcacctg aagcaggtgg
    17401 tcgtaatcta ggaccacggc ctatggaaat gattttactg ggtctgggtg gatgtactgc
    17461 gttcgatgtg gtaatgatat tgcaaaaagg aagacatcca gtaacagatt gtttcattga
    17521 aatcgatgca aaacgtgcaa cggaggatcc taaagtgttt acccaaattc atctccactt
    17581 catcgtatct gggaaaaatt tagcagcaca ccatgtcgaa agagccatcg acctctctgc
    17641 cgaaaaatat tgttctgcct cgattatgct gagaagtaca gccaatatca cacatgattt
    17701 tgaaatcatt gacgtctaac cccaaaactt ctaccttaca gactcagttt ttaactgtta
    17761 ctgcggtata gttttgatca taatcacgta aaattctcat caacaaccta aataatttat
    17821 gccatgaata acgataataa tgatgatcat aaatcggatg aatctttaca cgagaatatg
    17881 cgctattttc ttttctggat gatagagcag caagagttac tccatcaaaa gaatgtcaaa
    17941 aaagcgcagt gttttatcgc gcttattaga acgctgcatt ttgtcaaaac ccagccttgt
    18001 tcatcgaata ctcataaagt ttctacgcag tttatagcca gaaaattaaa tcagcttagc
    18061 tgtcattagc tacgataacc agtacggtta tgcaatttcc aatgagaaaa attctcagat
    18121 caagcatcag ccacgctgga tgttaaaaat agatcatgga aatgattacc gaacgaattg
    18181 ggaatgaatt agcaaagttc ttatcaaagc cagtaagaca aagcatccag gtcgcaatga
    18241 tctctcaaga acagctgctg gctacgctcc aacctgctga cgtacttctt gttgaaggca
    18301 atactcggat tagtactgcg attaagtatc tgacacaatc cagttggtct catgctgcgt
    18361 tatatattgg gaacgcatta cctaacgata gcccttggaa tttaccgcag gttctggttg
    18421 aagcggatct taaagaaggc atacgcgcag taaccattgc acagtatgca caaatgcata
    18481 cgcgaatttg ccgtcccatc ggtttaaatg atgatgatcg gaagcatctt attgattatg
    18541 cgattacgcg tattggtcaa caatatgatt taaagaatat ttttgatcta gcacgatact
    18601 tatttccagc cccccccatt ccaacacgat tccgaagaag attacttgcc ctaggcagtg
    18661 gagatccaac tcgcgctatc tgctcgacac tgatcgctca agcatttatg tctattcact
    18721 accccatcct gcccgaagtc caaagaacct ggtcagacaa tcctatgcat gccgattgcc
    18781 acgctgaaat ctatcatatc cgacatcaca gcttgtatac cccccgtgat tttgatattt
    18841 cccctttttt tgaaattatc aaaccgacga ctcgcgatca ctttgattac cacgctttaa
    18901 cttgggattc tgaaacagat aacacttaga tcatcagccg ccaaagtggc cagcactggg
    18961 ggagtcaact agtccactac tcctcatcag cattttcaaa acgaacccaa gaaccaccaa
    19021 cttgtgaacg ctcagcaccc atatcgaccg gtaaatctgc gatatcattt aatgttgcgt
    19081 cttgtgccaa aagatgtgca acatggacta caactaattc acttctcatt gcctcttcat
    19141 cttcgaagtc atggttcgca tcgtcacaat acatttgcca gtcaccgcct gcatgtgaaa
    19201 caaacaacac cggttttttg gcctcgagga cacatcggca cgtgataaca cctagattat
    19261 ctggaaataa catggaacct cccaattaac atagcgaaga tactcaaata atgctcatct
    19321 tacttagcca aaaaataaga tactcaaaag agctatcccg cttctgctca aaaataattt
    19381 agggacaatg cttgcgaatc acttcacttg cgacggtaat aatcattgcc aatgaaatag
    19441 ccccaggatc aggtgtaccg atgctctttt ctgcgtgagg acgcgcacga ccaatgcgcg
    19501 gcagcaaatt gcgggtttgt tgcgctgctg tattcgcaat atcagcagca tcttgccacg
    19561 cagttaccac tgaaataccc cttgcagaag cttgatcaag tgcagcggaa aaaggttgca
    19621 atacatccac aagagtcttg tcaccgggct gcgctttacc acgctgcatt acctcgcata
    19681 gtgcagcagc tacactacct gcaacagttg cagtatcagg tgctgttgta tcgcctaatt
    19741 taactccgaa tgtattaagt attacacccc acagcgcccc agacgtaccc cctgcgcgtg
    19801 cagcccaggc ctctccagac aaggttagta atgttccagc gccagcttgt gcatcatggg
    19861 cttttttcgc ggcatcaagc gctgctgata caccccttag cataccgata ccatgatcac
    19921 catcacccgc cacagcatcc agacgcccga gttcttcttg ttgggtttca atgatctgtt
    19981 tggccgatgc aaaaatctcc aggataatcg ctgcaatccg ttgcgaagca ggagaaccca
    20041 cagtcaccga ttgactggcc actgcaacct gcgggataag ggcagcatca gtctctggtc
    20101 taaagcatga aatagtgcct cgtttaaaag cgggtgtatc tgcggcagcc ttccagtaag
    20161 tttcgagttc atcatttagc cagaacaacg tcaacgaaat acccgccatg tcaaagctgg
    20221 taataacgcg atcaacaacc ggttcgacga taatcaatcc acgactgtgc aataactgat
    20281 caaccttacg ataaacaaca aacaattctt cctgcgctac actccccaag ctgctcaata
    20341 gcacactgat atgggtacct ttaggcaaat cgccaaccgg tgagatatct tgtagcaact
    20401 tctcgactaa catcgcagct aaatcatccg ctggaagccc atcttgctga tagagacccg
    20461 gttccccatg gatacccatc ccaactgcca attttccgtg ttcgacatga aataacggct
    20521 gttgagcacc gggtagtgtg cagccagaaa atgcgacgcc cagggtccga acacgtgtat
    20581 tggcttcatt ggcaatgcgg caaacttcat ccaacgattc tccactatca gcggctgcag
    20641 ccgccacctt gaataccggc aatgctccag cgataccacg gcgtagctgg cgctccgcta
    20701 gtggcgctga cgcaatatca tcggtcacta ccactgcacg cacatcaatt ccctgtgcct
    20761 gcaatcgcgc ttgcgcttga ttaaaattga gcacatcacc cgcataatta gcatagcaaa
    20821 gtatgatgcc accccctgca taagctgcca gagcaacatc atagatttgc tgcgcagaag
    20881 gtgacgcaaa tacattaccc attgcagcac catgtaccaa cccaggtcct accaatcccc
    20941 caaacgccgg ataatgccca gaaccccctc cgatgatgac aactacctgc ccgggttctg
    21001 cttggtaacg acgaaccaca ccaccgttga ctgcggcgat aagatcacgg tgggctgctg
    21061 caaacccttg aatcatctcc tctacaaaca tatcaggctg gttataaaga taagtcataa
    21121 tcaatcaaaa ttattagaag gaacgttata tgtatcacat attaaatcag aaaaaatatc
    21181 gattgattac cctgcgccac aaatactaac tctattctca aatagccgag ctacaaatca
    21241 tcttcaatta ttactgattc gtcagaaaaa aagtgcatga cattgctgtg gaaagctact
    21301 tgttcacatt tattatgctt tggtacgatt acaacaggtt tcatgacttc ttagagatac
    21361 gttgtattgc aagaccaaga ccaggtacct ttttgtgtta gttacgtttt agttacatag
    21421 ataaaattat caatctataa actaactgta acttattgat ttttggtgct gttgagagga
    21481 gtcgaacctc cgacctactg attaccaatt ctaaaatata ctgttttacg tagttttaat
    21541 attttttaga atgtattaat gtcattgttt tgtattgata tttcctatca tactgtttta
    21601 aactctaacc catagtttta cattttctgt ctacatagtg tctacatgga atgaaattaa
    21661 aacaatgact acttcccaaa ctctccccca aaaaatcaca aaatcttatg tagacaaatt
    21721 atctgttcca gaaacaggcc aagcttttat ccgcgatact gaactaaaag gttttgctgt
    21781 gcgaatcacc tcatccggtg caaaatcttt cattctagaa aaaagaatca acggcaaagt
    21841 aaagcgatta acaataggcc gctatcctga gataactgta gaacaagcca gaaaagaagc
    21901 tcacaaattg ctgggtcata ttgccacggg tcgtaatccc atcgctgaga aaaaacagga
    21961 agccttacaa ggcactacgc taaaacaggc tttcgatgat tttgttaaga cgcgaaaaaa
    22021 cctcaaagaa cgc
//
LOCUS       DAOQLJ010000021        21278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_850_length_21278_cov_6.169345, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000021 DAOQLJ010000000
VERSION     DAOQLJ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 21278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21278
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_850_length_21278_cov_6.169345"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1936)
                     /gene="oadA"
                     /locus_tag="PK522_02860"
     CDS             complement(92..1936)
                     /gene="oadA"
                     /locus_tag="PK522_02860"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HNC40707.1"
                     /translation="MRQEKSKQTVHITDVILRDAHQSLIATRMRTEDMLPACTLLDQI
                     GYWSLECWGGATFDTCLRFLKEDPWERLSQLKAALPNTPLQMLLRGQNLLGYRHYSDD
                     VVHAFIQRAATNGITIFRIFDALNDVRNLKTAIEAVKSAGKHAQGTICYTTSPVHDVK
                     SFVSLASDLAKLGCDSIAIKDMAGLLTPFATQELVSALKQTVDLPIHLHSHATSGLAE
                     MCQLKAIEAGCRHIDTALSSWSGGTSHPPTESLVMALQGTPYDTKLDLQKLQAVNDYF
                     AQIRKKYHRFESEFTGVDTRVHVFQVPGGMISNLANQLQERNALDRINEVYAEIPRVR
                     KDLGYPPLVTPTSQIVGTQAVLNILTGKRYSTITNEVKRYLQGGYGKAPAAINASLQK
                     SAIGKEDLIDCRPADLLKPEFEQLRKEIGQLALNDEDVLSYAMFPEIGKQFLEQRSSG
                     QLVPEPLEVASTTVNGVQKAPTEFNIALHGESYHIKVTGTSPKTESTRHFYFMVDGIP
                     EEILVETLDEIVLDGGTQGPVKSAIASKRRSPTEKGDVVVSMPCNILDVLVKIDQKIT
                     AGQPVLVTEAMKMETEITAPISGIVKAVHVVKGEAVNPNEVLIEIVAG"
     gene            complement(1951..3369)
                     /locus_tag="PK522_02865"
     CDS             complement(1951..3369)
                     /locus_tag="PK522_02865"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNC40708.1"
                     /translation="MLRKILIANRGEIAVRIIRACAEMGIRSVAIYSEADRFALHIKK
                     ADESHYIGNEPMAGYLNIHALVDLALATGCDAIHPGYGFLSENAEFAKVCKERGLVFI
                     GPTPEVIHRMGDKTEARNAMKAAGIPITPGSEGNVNSVKEALNVAEQIGYPVMLKATS
                     GGGGRGIRRCDTAQELERNYTRVISEATKAFGRAEVFLEKYIVNPKHIEVQILADHHG
                     NVIHLYERDCSIQRRNQKLIEIAPSPQLDEAQRQYIGGLAVIAAKSVGYTNAGTVEFL
                     LDDSGRFYFMEMNTRVQVEHTITETITGVDIVEEQIRVAAGLKLRFKQDEIVRRGYAI
                     QFRINAEDPKNNFLPSFGRISRYYAPGGPGVRTDTAIYTGYEIPPFYDSMVAKVIVSA
                     LTWEDVIKRGERTLRDMGLFGIKTTIPYYLKILNHPQFRAARFNTSFVEQNPQLINYS
                     NKPRPEVLASVIAAAVASHTGL"
     gene            3496..4593
                     /locus_tag="PK522_02870"
     CDS             3496..4593
                     /locus_tag="PK522_02870"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNC40709.1"
                     /translation="MKILLIIVLLSMLYSLSWPVPIEPESWKAPSAPGYTDQHAVNQR
                     LGQLNQIHLQGYEGPEHVAISPTGQLYTAVVDGSLLRLNPDGSDLEVFVNTGGRILGF
                     DFDVSGNIIAADALKGVLKITPDRRIEVLVDEVEGKPIRYPNSVVVASNGTIYFTDSS
                     SRFVPADWDGTFEATMLDIVEQSATGRVIAYDSLSGRTRVIAQGLSFANGIALSRDEQ
                     TLFVVETGRYRLWKIAILANAITIGDESPDASVLLDNLPGYPDNLMRGLEGRIWLGLA
                     KPRNTAIDFMSDKPLLRKMVLRFPRFLWPMPESYGHVIAFTETGQIVVDLQDPTGDYP
                     ETTGVTETNERLYIQSLHAKSLGWLDKSILK"
     gene            4609..5265
                     /locus_tag="PK522_02875"
     CDS             4609..5265
                     /locus_tag="PK522_02875"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC40710.1"
                     /translation="MKKQPDFIPALHYHWLTRWYDPVMRTIFPETRITDMLIRQANIQ
                     SGERVLDVGCGTGTHTIRVKRAQPEAMVHGVDIDPEVLKLAQRKIEQSHIELLLKQAS
                     ANALPYPDNHFDHIISSLLLHHLKPVFKQKMLEEAFRVLKPGGRLHLIDFGEPHDMSM
                     WVISLIVRWFEEIHSHIHGCLPKLIQEAGLNLMPMSLHFRTLGGTITLWHAYKPTEVY
                     "
     gene            5436..5747
                     /locus_tag="PK522_02880"
     CDS             5436..5747
                     /locus_tag="PK522_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40711.1"
                     /translation="MTTRKLTLTLALVGVLLLSGCVTTPYFSGVNGRYGNVSFSYGYR
                     PPVAISPGYYTSHRQVYVTPRYYNRGHGHFIGGHRHFGGGHKHFIGGHRGGFRGGHFR
                     W"
     gene            5934..6542
                     /locus_tag="PK522_02885"
     CDS             5934..6542
                     /locus_tag="PK522_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNC40712.1"
                     /translation="MLSIIQAAGWPIWPIIIASIVAAAIIGERIWSLRSSKVSPRDLL
                     SKVLHDYRRSGINASTLNRLQENSPLGCVLAAGLKNIESSREVMKESIEEAGRVVAHE
                     LDRYLTTLGTIASLSPLMGLFGTLIGMIEIFGSNSPTGSNPAQLAHGISIALYNAAFG
                     ILVAIPSMIFYRHFRAKVDALVLEMELQALKLVEVVHGDRKI"
     gene            6741..7760
                     /locus_tag="PK522_02890"
     CDS             6741..7760
                     /locus_tag="PK522_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC40713.1"
                     /translation="MNNKTCSLVTGGGGFIGMHLVKQLLDQGEAVKVLEISDTTFPKE
                     VEVIQGSITDVDTVRFALKGVDRLFHLAANPYLWAHNKKIFDQVNYEGTRVVLSEAKR
                     ANIKKVVHTSTESILSTKTSKNQPIHSSVVPILEDMPGPYCRSKFLAEQEAFKASQEG
                     LPVVIVSPTLPIGPGDRRLTPPTQMILNFLNHKIPAYLDCKLNVVDVRDVARGHILAA
                     ERGRPGERYILGNQNIQLSKLLQLLEEISGLVMPKTKIPHWLALIFSVFSELTADYIT
                     RKPPQAPLTGVRLAGRSLFFESDKAVRELGFKQSSIRQALIDQVEWLLQEGYITRSMP
                     QRQRV"
     gene            7858..8739
                     /locus_tag="PK522_02895"
     CDS             7858..8739
                     /locus_tag="PK522_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNC40714.1"
                     /translation="MKARCNTIILLGIFITALMACSSAPKKESLADTQQVTAHQGSNA
                     EIHSEHSEEMPVDPYESANRKTYSFTDSIDRFILEPVADVYIDYVPYAIQRPISNFYR
                     NLSYPNVALNAFLQGKFRQGFEDSFRFVINSTIGIFGLADMAGHMGFKENNEDFGQTL
                     AVWGVDPGSYLFVPIYGPSNRRDILDLPIGFFTDALFYASYAISGPALVPILFLRVVD
                     KRARLAGPMRIRDESALDPYLFVREAYTQQREYLIHDGNPPIEQYDDIEEEGDEKDKV
                     KDAVKKEKIEDAKSNKD"
     gene            8876..10867
                     /gene="shc"
                     /locus_tag="PK522_02900"
     CDS             8876..10867
                     /gene="shc"
                     /locus_tag="PK522_02900"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HNC40715.1"
                     /translation="MSRISQQFNDLINLKKIALKDTWFKSKATSPTSSSDVAQSELVQ
                     CITKARNALMALQNKDGHWCFPLEADCTIPAEYILMMHFMDEIDVELEQKLAIFIREQ
                     QSEVHRGWALYYGGEFDLSCSVKSYFALKIVGDSPDAPHMQRVRAAILERGGAASANV
                     FTRLLLAMYQQIPWRGVPFVPAEIILLPRWFPFHLRKVAYWSRTVMIPLSILCTLKAK
                     AANPRKIGIRELFTIPPEEEQNYFPVRTSLNRILLYVERLGSKLEPMIPTFIRKIALK
                     RAEKWVIERLNGECGLGAIFPAMVNAYEALTLLGYDAEHPYRKQCKAALQGLLVDEGR
                     RAWCQPCTSPIWDTVLASLSLQEDSHADQEPVKQALDWTIQRQILDEPGDWREAKPNL
                     PGGGWAFQYENPHYPDLDDTAAAAWALYQADPEVYRFSLRRAADWLAGMQSSNGGFAA
                     FDIDNTYYYLNEIPFADHGALLDPPSSDVTARCTGFLSLYGDPQHQQVVERGLAYLFN
                     EQESNGSWFGRWGSNYIYGTWSVLEAMQLAKVDRQHEAIRKAVQWLKSKQRPDGGWGE
                     TNDTYLDPRQAGEFNVSTSFQTAWALLGLMAAGEVNSTAVQKGISYLMKTQADDHLWY
                     EPWFTAPGFPRVFYLKYHGYSKYFPIWALIRYRTLTTER"
     gene            10873..11610
                     /locus_tag="PK522_02905"
     CDS             10873..11610
                     /locus_tag="PK522_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HNC40716.1"
                     /translation="MSLIGIVTAMRAEAKCVTSRRLPFNEMVRLREGTAIWICGIGEA
                     AAHKAAIGLRDQGVSALVSFGVAGALSASLRPGDLVLPEHIQIEESDRVSVALAWRAR
                     IKQLLPKHVNVAGGTLATSRQMLSSEAAKRAFSETHGACAVDMESGAIAKVAAETGIP
                     FVAVRAITDPLEFSPPPILMDAVYPDGSVNIPQLLNLLIRRSINISTLLQLAPSMRAA
                     CSTLLSVAQHADNELGGQFSSVAIANS"
     gene            complement(11605..12789)
                     /locus_tag="PK522_02910"
     CDS             complement(11605..12789)
                     /locus_tag="PK522_02910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNC40717.1"
                     /translation="MPSSNLPQPTQEGLQHSQHLQSYIVNKIHESGGWIPFAQFMELA
                     LYTPGLGYYSASNVKFGFNGDFVTAPEISALFGRTLAKQAMQLFHQIGKTSILEFGAG
                     SGKLALDLLMELEQFNQLPEQYLILEISAELQQRQRMLFETHAPQFLDRITWLAQLPD
                     QFSGLIIANEILDAMPTHLILWQKEALFERGVSANQQTFSWVDKPLNHDELLTIANQL
                     SVFIERDRSDFVSYLSEVSLANRYFIRSLSEILQAGLILLIDYGFGQREYYHPQRSMG
                     TVMCHYRHFAHDDPFYLPGLQDITSHVDFSAIAQAASESNLQLLGYTNQAHFLINCGI
                     TELLSSVAVTDTLKYLPLSNQLQRLVSPAEMGELFKVIALGKRLSEIPVGFTRGSLLH
                     LL"
     gene            12869..13615
                     /locus_tag="PK522_02915"
     CDS             12869..13615
                     /locus_tag="PK522_02915"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HNC40718.1"
                     /translation="MTSNNKVILITGGAKRVGAAICRQLHAQGANLAIHYRSSQEEAE
                     ALRDSLEAMRRQSVLLIEADILNVDQLPNLIEQIILRFGQLDAVVNNASSFFSTPIGR
                     FSDAAWNDLIGSNLKAPLFLSQAAAPYLIKQKGCIINIVDIHVDYPLKNHVIYNCAKG
                     GLAALTRSLALELAPDVRVNGVSPGPILWPVTGDWADTSLQHEIINRTLLKRCGEPDD
                     IAKAVNYLINDAPFVTGQIIAVDGGRSINL"
     gene            complement(13621..13959)
                     /locus_tag="PK522_02920"
     CDS             complement(13621..13959)
                     /locus_tag="PK522_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNC40719.1"
                     /translation="MNGSEAEDLAAHYLQQQHLVLVERNYRCRYGEIDLIMRDQTMLV
                     FIEVKMRTHTSFGGAIHSITESKQHKLILTANHYLANLKKIPPCRFDVVLITGQLTHE
                     INWIKNAFDE"
     gene            14061..14912
                     /gene="rsmI"
                     /locus_tag="PK522_02925"
     CDS             14061..14912
                     /gene="rsmI"
                     /locus_tag="PK522_02925"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNC40720.1"
                     /translation="MQQNNGTLYVVATPIGNLRDISLRALDIFALVDRIAAEHADLTR
                     HLLSHHGIKTPLMTLHQHNEHLAVEKIANLLANNQDVALVTDAGTPCISDPGAMLVHA
                     MRALGHKVVPIPGANAAICALSASGIRSTSFFFYGFLPVKSGERQRCLASLTQLLSCT
                     LIFYEAPHRIVDCINDMIAVFGAHRQITLGRELTKIFETFHTDNLENTLQWLLADENQ
                     QKGEFVLLLPSFLEENEPGLSRDAQHTVQVLMKELPLKQAVQLAVSITGEKKKSLYAY
                     ALSIKND"
     gene            14944..16002
                     /gene="pyrC"
                     /locus_tag="PK522_02930"
     CDS             14944..16002
                     /gene="pyrC"
                     /locus_tag="PK522_02930"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNC40721.1"
                     /translation="MSQTITIIQPDDWHLHLRDGEQMQCVLPDSAIRFARAVIMPNLK
                     PPIITTDQAIQYRSRIIESLPNALRSHFNPLMTLYLTDNTSPQEVAKAKKSGMVHAVK
                     LYPAGATTNSDAGVTDLSKCFATLASMEQLGLPLLVHGEVTDPAVDIFDRESVFIDRV
                     LTPLIRRFPVLRIVLEHITTREAVEFIRSTPNHIAATITAHHLLFNRNAIFTSGIRPH
                     YYCLPILKREKHRQSLVAAAISGSPKFFLGTDSAPHSIANKESNCGCAGIYSAHAGIE
                     FYVEAFDRAGALDKLEAFASFHGADFYQLPRNEHQITLIKQDWIIPAQIRFGDEQLIP
                     LMAGEKLSWKLSNHFLAW"
     gene            16199..16801
                     /gene="rpoE"
                     /locus_tag="PK522_02935"
     CDS             16199..16801
                     /gene="rpoE"
                     /locus_tag="PK522_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HNC40722.1"
                     /translation="MSDREIDQQLVERVQQGDKRAFDLLVVKYQRKLARLLSQLIRDP
                     VEVEDIAQETFIKAYRALPSFRGDSAFYTWLYRIGINTAKNFLVSQGRKVPTIEGNFD
                     NEDAENFEEAAKYKEVSTPESELMSKQVAQTVNKTLDSLPEELRTAIVLREIEGLSYE
                     DIASMMGCPIGTVRSRIFRAREAIAEKLRPLLGTSKDKRW"
     gene            16815..17363
                     /locus_tag="PK522_02940"
     CDS             16815..17363
                     /locus_tag="PK522_02940"
                     /inference="COORDINATES: protein motif:HMM:NF015810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HNC40723.1"
                     /translation="MKEKMSELMDGELDNRNIDSVFTEFKRNDELIKDWETYHMISDA
                     IKRPASFQSFDIADKVRKQLNHEPIIFVPNASKVAKHKLYTLSAAASVAVLVGSWLLL
                     QIENIQPVTTVAEKTKERILVSQSPASQPTPTFTYTLPSLEYHYFHHQPQPLARKVFL
                     PNGAMYGPLTSEYQVPEAGNSR"
     gene            17523..18917
                     /locus_tag="PK522_02945"
     CDS             17523..18917
                     /locus_tag="PK522_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNC40724.1"
                     /translation="MQKFLLSLLFVTSIGIVNAKELPDFTGLVEQYGAAVVNISTIQT
                     QQISGGQIFPDIPNLPEDSPFYDFFRRHMQPFSAPRKFESRSLGSGFVISKDGYILTN
                     AHVVESANEITVKLNDKREFNAQVIGTDRKTDIALIKIDAKDLPVVTQGDPENLKVGE
                     WVIAIGSPFGFENSVTAGIVSAKGRSLAQENYVPFIQTDVAINPGNSGGPLFNMKGEV
                     VGINSQIYSRTGGFMGLSFAIPINVATEIADQLKEHGKVSRGKIGVMIQEVTEELAES
                     FGLSKATGALVVSIEKGGPADKAGIEVRDIILEFNGKPIANSSDLPRIVGNTKPKSEV
                     TTQVWRKGSTQSLTVLVGEMPIEDKSERIQKQGKVPDKANRLGLALSELTDKLKKQLG
                     VEGGLLVEEVQPGTASRVGIRAGDIILGLNSQDIKTVKQFNQMLDKISKGKNIALLIK
                     RGDMTTFITMKLDN"
     gene            18947..19204
                     /locus_tag="PK522_02950"
     CDS             18947..19204
                     /locus_tag="PK522_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNC40725.1"
                     /translation="MDQPNLIVYVREACHLCQEMVALLHQYQQKTAFKLEIIDIDDDP
                     RLVQQYNELIPVLISADNQQSEICHHHLDITALDAYFNKIR"
     gene            19318..21108
                     /gene="lepA"
                     /locus_tag="PK522_02955"
     CDS             19318..21108
                     /gene="lepA"
                     /locus_tag="PK522_02955"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNC40726.1"
                     /translation="MRNIRNFSIIAHIDHGKSTLADRIIQYCGGLSEREMEDQVLDSM
                     DLERERGITIKAQTVALKYVGRDGQAYLLNLIDTPGHVDFSYEVSRSLAACEGALLVV
                     DASQGVEAQTVANCYTAIEQGVEVLPVLNKIDLPAADPDRVISEIEDIIGIDAKHALR
                     VSAKTGEGIQDMLEAIIDRIPEPQGDSSAPLKALIIDSWFDNYVGVVVLVRVVDGVLK
                     PGDKIMLMATRATQLCEQVGVFLPKAEHRDALSAGEVGYVISGIKELKSAKVGDTITL
                     VDSPANEPLTGFKEIKPQVFAGLYPVESNQYEALRAALEKLQLNDSSLQYEPETSQAL
                     GFGFRCGFLGLLHLDIVQERLEREYDMDLITTAPTVIYQVLMRDGSEIEIENPSKMPD
                     LSKIAEIREPIITTTILVPEEYVGTVMLLCNGKRGVQRNMQYMGRQVMLVYEMPLNEV
                     VMDFFDKLKSVSRGYASLDYEFKEFRASDLVKLDILINAEKVDALSLIVHRSSSQYRG
                     RELAQKMRELIPRQMFDIAVQAAIGAHVIARENIKALRKNVLAKCYGGDITRKRKLLE
                     KQKAGKKRMKRVGNVEIPQSAFLAILQVDK"
BASE COUNT         6062 a         4127 c         4862 g         6227 t
ORIGIN      
        1 tgttctttgt gcttttgccc tttgcggggt gcaggggcgg agcccctgcg ggtaattttt
       61 tagggttaat ttattggaaa ttctagcaag cttatcctgc cacgatttca attagcactt
      121 cattggggtt gactgcttca cctttgacca cgtgcacggc tttgacaata ccgctgatcg
      181 gtgcggtaat ttccgtttcc attttcatgg cttcagtcac taataccggt tgtcccgcgg
      241 tgattttctg gtcgattttg acgagtacat cgagaatatt acacggcatg ctgaccacca
      301 catcaccttt ttctgtaggg ctacggcgct tgctagcaat tgcgcttttc acagggcctt
      361 gagtgccgcc atcgagtacg atttcatcga gcgtttcaac caagatttct tcgggaatgc
      421 catcgaccat gaaataaaaa tggcgcgtgc tttcggtttt aggactggta cctgtgactt
      481 tgatatggta ggactcaccg tgcagcgcaa tattgaattc agtcggtgct ttttgcacac
      541 cattgacggt tgtcgaagcc acttcaaggg gttcaggaac cagttgaccc gatgaacgtt
      601 gttccagaaa ttgcttgccg atttccggaa acatagcata actgaggaca tcttcatcgt
      661 ttaaggcaag ctgtccgatt tccttgcgca gttgctcgaa ttcaggtttt aacaaatcag
      721 ccgggcggca atcgatcaaa tcttccttac caatcgcact tttttgcaaa ctggcattga
      781 tggcagcagg cgctttgcca tacccgcctt gtaaatagcg tttgacttca ttggtgatgg
      841 tggaatagcg ttttccggtc aaaatattga ggacggcttg cgtgccgacg atttgcgaag
      901 tcggagtgac cagcggcggg tagcccaaat ctttgcggac tcgtggaatt tccgcataga
      961 cttcattgat acgatctagc gcgttgcgtt cttgcagttg attggccaaa ttggaaatca
     1021 taccacccgg aacttggaat acatggacac gcgtatcaac tcccgtgaat tcgctttcaa
     1081 atcgatgata ttttttacgg atttgcgcaa aataatcatt cacggcttgc aatttttgca
     1141 ggtcgagctt ggtgtcgtag ggcgtacctt gtaatgccat cactaaactt tcagtcggcg
     1201 ggtggctggt accaccggac catgaagaaa gcgccgtatc gatatggcgg caaccggcct
     1261 cgatggcttt gagttggcac atttcagcta gccctgatgt tgcatgcgaa tgcagatgaa
     1321 tcggtaaatc gacagtctgt ttcaatgcgc taaccaattc ctgtgtcgca aagggggtaa
     1381 gtagccctgc catatctttg atggcaatcg agtcacaacc gagtttagca agatcgctag
     1441 ccaagctgac aaagctttta acatcatgaa ctgggctggt ggtatagcaa atcgtacctt
     1501 gcgcatgctt accggcagat ttaactgctt cgatggcggt tttgaggtta cgtacgtcgt
     1561 tgagcgcgtc aaaaatccgg aaaatcgtga ttccattggt agcagcgcgt tggataaacg
     1621 catgcacaac atcatcggag taatggcgat aacccagcaa attctggccg cgcagtaaca
     1681 tttgtagtgg tgtattgggt aacgctgctt tgagttggct caaccgttcc cagggatctt
     1741 cttttaaaaa acgcaagcac gtatcaaacg ttgcacctcc ccagcattcc aacgaccaat
     1801 aaccgatttg gtcgagcagg gtgcaggctg gtaacatgtc ttccgtacgc atccgggtgg
     1861 caatcaacga ttggtgtgca tcgcgcagaa tgacgtcggt gatatgaacg gtttgcttgg
     1921 atttttcttg gcgcatagcg tttgaataga ttagagcccg gtgtgcgatg caaccgcagc
     1981 ggcaatgacg cttgctagca cttcagggcg aggtttattg gaataattga tcaattgtgg
     2041 attttgttcg acaaaactgg tattgaagcg cgctgcacga aattgcggat ggtttaagat
     2101 tttcaaataa tagggaatgg tcgttttgat tccaaataac cccatatctc gtagcgtacg
     2161 ttcgccgcgt ttgatgacat cttcccaagt aagggcgctg acaatcactt tggccaccat
     2221 ggaatcatag aaaggcggaa tttcatagcc ggtataaatc gcggtatcag ttcgaacgcc
     2281 agggccaccg ggtgcatagt aacgcgaaat acgtccaaaa ctcggtagaa agttgttttt
     2341 gggatcttcc gcattgatgc ggaattggat tgcgtaaccg cgacgcacga tttcatcttg
     2401 cttgaagcgc aattttaatc cggctgcaac acgaatttgt tcttcaacga tatctacacc
     2461 cgtgatcgtt tcagtaatgg tgtgttcgac ttgcacgcgc gtgttcattt ccatgaaata
     2521 aaaccggcca ctatcatcca gcaaaaattc aacggtaccc gcgttagtgt atccaacgga
     2581 tttagccgcg atgacagcta acccaccgat atattggcgc tgcgcttcat cgagttgggg
     2641 agatggtgca atttcaatta acttctgatt acggcgctga atcgagcaat cacgttcata
     2701 gagatgaatt acattcccat gatggtccgc aagaatttgt acttcgatat gtttaggatt
     2761 gacgatatat ttttccagaa aaacttcggc acggccaaac gctttggtgg cttcggagat
     2821 aacgcgcgta tagttgcgtt ctaattcctg tgcagtgtca cagcgacgga taccacgtcc
     2881 accaccccca gaggtggctt tcaacatcac gggatagcca atttgctcgg caacgttgag
     2941 cgcttctttg acagaattta cgttgccttc tgaacccggt gtaatcggaa taccggctgc
     3001 tttcatggca ttgcgtgctt cggtcttatc acccatgcgg tgaatgactt ctggggttgg
     3061 gccgatgaaa accaaacccc gttctttgca cactttagca aattcagcat tttcagagag
     3121 aaaaccatag cccggatgaa tggcatcgca gccagtagca agcgctaaat caaccaaagc
     3181 atggatattt aggtaacctg ccatcggttc attcccaatg taatgggatt catcggcttt
     3241 cttgatatga agcgcgaacc gatctgcctc agaataaatg gcgaccgagc gtatccccat
     3301 ttccgcacaa gcgcgaataa tgcgtaccgc gatttcccct cgattcgcaa ttaaaatttt
     3361 acgcagcata tcaaaattga atgaaataaa attgagtgtg gttattttct agtgattgct
     3421 tttcagaact gttgaacttt tgtacatttt aatgggtgtg tcaatatttt ttagtagtga
     3481 tgggatgaaa aagcaatgaa gattttatta ataatcgtct tgttatcaat gctttattcg
     3541 ctaagttggc cagttccgat tgaacccgaa agttggaagg caccgagtgc acccggttat
     3601 accgatcagc atgcggtaaa tcagcgattg ggacaattaa atcaaatcca tttacagggc
     3661 tacgaggggc cagagcatgt tgctatctca ccaaccggtc aattgtatac agccgtcgtg
     3721 gatggaagtt tacttcgttt gaatccggat ggtagcgatt tagaagtgtt tgtgaatacc
     3781 ggcggtagaa ttttgggatt cgattttgat gtttcgggta acatcatcgc agcggatgcc
     3841 ttgaaagggg ttttaaaaat tacccctgat cggcgtatcg aggtattggt cgatgaagtg
     3901 gaaggcaagc ctattcgtta tcccaatagt gtcgtggttg caagtaacgg tacgatttat
     3961 tttaccgatt catccagtcg ttttgtaccc gctgattggg atggaacttt cgaagcgaca
     4021 atgctggaca ttgtcgaaca gtctgccaca gggagggtga ttgcctatga ttcactgtca
     4081 ggtagaacga gagtcattgc acaagggttg agttttgcca atggaatcgc gctatcgcgc
     4141 gatgagcaaa cattgtttgt cgttgagacg gggcgttacc gcctatggaa aattgcaata
     4201 cttgcgaatg ccattacgat tggtgacgaa tctcctgatg cttccgtgtt gctcgataat
     4261 cttccagggt atccggataa tttaatgcgt ggactggagg gaagaatttg gctgggctta
     4321 gctaaaccac gcaataccgc cattgatttt atgagcgata aaccgctatt gcgcaagatg
     4381 gttttacggt ttccgcgctt tttatggcca atgcccgaat cctatgggca tgtgattgca
     4441 tttactgaaa ccgggcagat tgttgtggat cttcaagacc caaccggtga ctatcctgaa
     4501 acaactggtg ttaccgagac gaatgagcgg ttgtatattc agagcctgca tgcgaaaagc
     4561 ttgggttggc ttgataaatc aatcttgaaa tgaaacctta atcctacgat gaagaaacag
     4621 ccagatttca tacctgcttt acattatcac tggttaacgc gttggtatga tcccgtgatg
     4681 cgaacgatat tccctgaaac acggataacc gacatgctaa ttcgccaagc aaatatccaa
     4741 tcgggagaac gtgtattgga tgtaggatgc ggaactggta cccatactat ccgagtcaag
     4801 cgagctcaac ctgaagcgat ggttcatggt gtagatatcg atccagaggt gttaaagcta
     4861 gcgcagcgaa aaatcgaaca aagccatatt gagcttttgc taaagcaagc atcggcaaac
     4921 gctttacctt atcctgataa ccattttgat catattatta gcagcttact gttgcatcac
     4981 ctaaaacctg tatttaagca aaaaatgcta gaagaagcct ttcgagttct gaaacccgga
     5041 ggtcgattac atctcattga ttttggtgaa ccccatgata tgtccatgtg ggtgatttca
     5101 ttgattgttc gttggtttga ggaaattcac agtcatatcc atgggtgttt acccaagttg
     5161 attcaagaag cgggtttaaa tttaatgcca atgtcgcttc atttccgtac gttgggtggc
     5221 accattacgt tatggcatgc ctacaagcca actgaagttt actaaataaa tatcaacatt
     5281 tagtgcaaaa atcttttcat cacttgaatt gcttattcag gtgatattca gctcaagcga
     5341 ctcacaatat ctctaaatcc tgagaggatc aggataataa gtgcagtgag tttaagggtt
     5401 actcgattaa cccttgcgtt ttgaggagat gaatcatgac tactagaaaa ttgaccttaa
     5461 ccttagcgtt agttggagtg ctgttattga gtgggtgcgt aacaactcct tacttctcgg
     5521 gtgtgaatgg gcgatatggc aatgtttctt ttagttatgg ctatcgtccg ccggtagcca
     5581 ttagtccagg ttattacacg agccatcgcc aagtgtacgt aacaccgcgg tactataatc
     5641 gtggccatgg tcatttcatc ggtggtcatc gtcattttgg cggcggccat aaacatttta
     5701 tcggtggaca tcgaggagga tttcgtggtg gccattttcg gtggtgatat ctcgcgctgg
     5761 tggtaatgta gtcttaagct ttcttattgg gggtatggat ggtatcggat tgatatcttc
     5821 ataccctatc aaatcgataa catttggata gtaaaagtag agttcattgc aaaggcaagt
     5881 tagaatagtc agtatttgct tgatagaacc ttttttcatc aggagatttt tttgtgttat
     5941 cgatcattca ggctgcgggg tggccaatat ggccaattat tattgcttcc atcgtagcgg
     6001 ctgcaattat tggggaacga atttggagtt tgcgatcaag taaggtatcg ccgcgagatt
     6061 tgttatccaa agtgttgcat gattatcgcc ggtcaggtat caatgcgagt acgttgaatc
     6121 gattgcaaga aaactctccg cttggttgtg tcttggcggc tggtcttaaa aatatcgaaa
     6181 gttcgcgtga agtgatgaaa gaatcgattg aagaagcggg acgtgtcgtc gcccatgaat
     6241 tggaccgata tttgactacc ttgggaacga ttgcatcctt gagtccattg atggggttat
     6301 ttggaactct tattgggatg atcgaaattt ttggttctaa ttcaccaacc ggtagcaatc
     6361 cagctcaact tgctcatggg atttcgattg ctttatataa tgcggcattt ggaattctag
     6421 ttgctattcc aagtatgatt ttttatagac acttccgtgc aaaggttgat gcattagtct
     6481 tggaaatgga gttgcaagca ttaaaattgg ttgaagtcgt tcatggggat cgaaaaattt
     6541 aacttgcaac aaatactatc cagtaaggtt ttagtctgat tgatagattg tttctaatat
     6601 tacagtcagt aatagaaatt gatttactaa tatcgacgat cacttaatat aagcagtttt
     6661 agaaaattca caaataattt atagttctgc gtgtggatca tctgattttg tacacgtaat
     6721 tgaattaaag aggatttatt ttgaataata aaacttgctc attggtaacc ggtgggggtg
     6781 gatttatcgg catgcatttg gtcaaacaat tattagacca aggcgaggcc gtcaaggtgc
     6841 tggaaatttc tgacacaacc tttcccaaag aagtagaagt gattcaagga tctatcactg
     6901 acgtggacac tgtccgcttt gcattaaaag gggtggatcg cttatttcac ctggcagcca
     6961 atccctatct atgggcgcac aataaaaaaa tattcgatca agttaattat gaaggtacgc
     7021 gcgttgtgtt atctgaagct aaacgcgcga atataaaaaa agtggttcac acctcgactg
     7081 aatcgattct ttccacgaag acttcgaaaa atcaaccgat tcattcttca gtggtaccga
     7141 ttcttgaaga tatgcccgga ccctattgtc gctcaaaatt cttagcagag caggaagctt
     7201 ttaaagcttc acaggaaggt ttaccggtgg tcattgtttc tccaacgcta ccgataggac
     7261 ccggtgatcg tcgattaacg cccccgacac agatgatttt aaatttccta aatcataaaa
     7321 ttccagctta cttagactgt aaattgaatg tggttgatgt aagagatgtt gcacgaggcc
     7381 atattttagc agcggaacga ggccgccccg gtgagcgcta tatcctcggt aatcaaaata
     7441 tccaattaag caaattgttg cagttgctgg aagaaattag cggtttggta atgcctaaaa
     7501 ctaagattcc gcactggcta gcgctcattt tcagtgtttt ctcagaactg acagcggatt
     7561 atattactcg taagccgcct caagcaccgt taaccggtgt aagattagcg ggtcgttcat
     7621 tattttttga aagcgataaa gcagtgcgtg agttaggttt taaacaaagc tctattcggc
     7681 aggcgttgat cgatcaagtt gaatggctat tgcaagaagg ctacataaca cgatcaatgc
     7741 cacaaaggca acgcgtataa tcatggatgt gtacgcagtt gttttaggta agttgaaaaa
     7801 tatcagtaaa ttaatattaa atttagtttc ttatcgatca cttttgaatt ctgtagcatg
     7861 aaagcacgct gtaatacaat tattttattg ggtatattca ttactgcttt gatggcttgt
     7921 tcgtcggcac ctaagaagga atcattggca gatacccagc aagttactgc tcatcaggga
     7981 tcaaatgccg aaattcattc cgagcactca gaagaaatgc ccgttgatcc ctatgaatcg
     8041 gctaaccgta aaacctattc gtttaccgat agcattgatc gatttattct agagccagtt
     8101 gcggatgtgt atattgatta tgttccctat gcaattcaac ggcccataag caatttttat
     8161 cgcaatttat cttatcctaa tgttgctttg aatgcttttt tgcaagggaa atttcgtcaa
     8221 ggatttgaag atagctttcg ttttgtgatt aactctacga ttggtatctt tggcctagct
     8281 gatatggctg gccatatggg atttaaagaa aataatgaag attttgggca gacacttgca
     8341 gtttggggag ttgatccggg gtcttacctt tttgttccaa tctatggtcc aagtaatcgc
     8401 cgggatattt tggatttacc gataggattc tttaccgatg cgctatttta tgcaagctat
     8461 gcaattagtg ggcctgcgtt agtaccgatt ctatttttac gagtggtcga taaacgcgca
     8521 agattggctg ggccgatgag gatacgagat gaatcagcat tagaccctta cttgtttgtt
     8581 cgagaagcct acacgcaaca acgcgaatat ctgattcatg atggcaatcc tcctattgaa
     8641 caatacgatg atattgaaga ggaaggtgat gaaaaagata aagtgaagga tgcagttaag
     8701 aaagaaaaaa tagaagatgc taaaagtaat aaagattaat aattatcacg tttctccgcc
     8761 tagctgtatc gtatatacga cagtccgttg tttttttcat taataaacag tgactgaatt
     8821 aacgtttctt gtaaaatgct ttgattacaa ttagttaacg gtaaggcttt aatttatgtc
     8881 tagaatatca cagcaattca atgacttaat taatttaaag aaaattgctt tgaaagatac
     8941 atggtttaag tcgaaagcaa cttcaccaac ctcatctagc gatgttgcgc aatctgaatt
     9001 agtgcaatgc atcacaaaag cgcgtaatgc cttaatggct ttacaaaata aagatggaca
     9061 ttggtgcttt ccgctggaag cggattgtac gattcctgca gaatatattc tcatgatgca
     9121 tttcatggat gaaattgatg tcgagttaga gcaaaaactc gctattttca ttcgagagca
     9181 gcaaagcgaa gtacataggg ggtgggcact ctattatggt ggggaatttg atttaagttg
     9241 tagcgttaaa tcgtattttg cattaaaaat cgtcggcgac tcgcctgatg cgccgcatat
     9301 gcaacgtgtg cgtgcagcga ttcttgaacg cggtggcgcg gcaagtgcta atgtgtttac
     9361 gcgcttactg cttgccatgt atcaacagat accttggcgg ggtgtgccgt tcgtgcctgc
     9421 agaaattatt ttattaccac gctggtttcc atttcattta aggaaagtag cgtattggtc
     9481 gcgaacggtc atgataccgt tgtctattct ttgtacgctg aaagcaaaag cagctaaccc
     9541 tcgaaagatc ggtatcagag aattatttac tattccgccc gaggaagaac aaaattattt
     9601 cccagttcgc acatcactca atcgaatttt gttgtatgtg gaacggttgg gctccaagct
     9661 cgaaccaatg ataccgacat tcatcagaaa aatagcactc aaacgcgctg aaaaatgggt
     9721 cattgagcgt ttgaatggtg agtgtggttt aggtgccatt tttccagcca tggttaatgc
     9781 atatgaagcg cttacattac ttggttacga tgcggagcat ccttatcgta aacaatgtaa
     9841 agcagcgcta cagggattat tggttgatga aggcagaagg gcatggtgtc agccttgcac
     9901 ttcgccgatt tgggatactg tgttagcgtc cttatcattg caggaggact cccatgcaga
     9961 tcaggaacct gttaagcaag cgctggactg gacgatacag cggcaaattc tcgatgaacc
    10021 aggtgattgg cgtgaagcaa agcccaattt accggggggt ggatgggcct ttcaatatga
    10081 aaacccccat tacccagatt tagatgatac tgctgccgca gcatgggcat tgtatcaggc
    10141 tgatccggaa gtatatcgat ttagcttgcg acgtgcagct gattggcttg caggcatgca
    10201 atcaagtaat ggcgggtttg cagctttcga tattgataac acctattatt atctgaatga
    10261 aattccattt gctgatcatg gtgcgttact tgatccaccc tcaagcgatg ttacagcgcg
    10321 ctgcacaggt ttcttatctt tatatggcga tcctcaacat cagcaggtag ttgagcgtgg
    10381 tttagcttat ttgtttaatg aacaagagtc caatggatcc tggtttgggc ggtggggaag
    10441 taactatatt tacggtacgt ggtcggtatt agaggcgatg caacttgcca aagtagaccg
    10501 ccaacatgaa gcaattcgga aagcggtaca atggctgaaa tcaaaacaac gacctgatgg
    10561 tggctgggga gaaactaatg atacttacct tgatccacgg caggcagggg aatttaatgt
    10621 aagcacttcc ttccaaacag cctgggcttt attaggtttg atggcagcag gcgaggttaa
    10681 tagtacagca gttcaaaaag gaattagcta tttaatgaaa acccaagctg atgatcactt
    10741 atggtatgaa ccttggttta cagcgcctgg ttttccgcgg gttttttatt tgaaatatca
    10801 cggttactcg aaatattttc ctatatgggc actaatacgc tatcgcactt tgactactga
    10861 gaggtaacag ttgtgagtct aattggcatt gttactgcga tgcgtgctga agctaaatgt
    10921 gtgacgtcgc gacgtctacc tttcaatgaa atggttcgct taagagaagg tacagcgatt
    10981 tggatttgtg gcattgggga agctgctgcc cataaagctg caatcggact tcgagatcaa
    11041 ggtgtatcag cgttggtgag ttttggtgtt gcgggtgcat taagtgcatc attacggccc
    11101 ggtgatctcg tattacctga gcacattcaa attgaagaat ccgatcgagt ttcagtggcc
    11161 ttagcatggc gggcccgcat aaagcaacta ttaccgaaac atgtcaatgt tgccggagga
    11221 actttggcta ccagtcgaca aatgctatca tctgaggcag caaagcgagc attctctgaa
    11281 acgcacggag cctgtgctgt cgatatggaa agtggcgcaa ttgctaaagt agccgcagaa
    11341 accggcatac cattcgttgc agtacgtgca attaccgacc cattagaatt ctcacccccg
    11401 ccaattttga tggacgcagt atatcccgat ggaagcgtaa atattcctca attattgaat
    11461 ctgttaatac gtcgctcgat caatatcagc acattgctgc aattggcgcc aagtatgcga
    11521 gctgcttgct cgacactgct ttcagtagcg caacatgctg ataatgagct cggtgggcaa
    11581 ttttcttcag ttgcaatcgc aaactcataa caaatgcaat aagctacctc gggtaaaacc
    11641 gacgggtatt tcactcaatc gcttgcccag ggcaattacc ttaaatagct cccccatctc
    11701 agctggactt actagtcgct gcaattgatt tgataacggt aaatacttta gtgtatcagt
    11761 gactgctaca ctcgataata attcagtaat accacagtta attagaaagt gggcttgatt
    11821 ggtgtagcct aacaattgta agttactctc agacgcagct tgagcaatgg ccgaaaaatc
    11881 aacatggctc gtgatatctt gtaaccctgg taaatagaat ggatcgtcgt gagcaaaatg
    11941 gcggtaatga cacatgacag tacccatcga ccgctgggga tgatagtatt ctcgttggcc
    12001 gaaaccataa tcgatgagca aaatcaatcc ggcttgcaat atttcactta agctccgaat
    12061 aaaatatcga ttggcaagtg aaacctcact taggtagctt acaaaatcag atctatccct
    12121 ttcaatgaat accgacaatt ggtttgcaat tgttagtagt tcatcatggt tcaaaggttt
    12181 atctacccaa gaaaatgttt gttgattggc actgacaccg cgctcaaaaa gcgcttcctt
    12241 ttgccaaaga attaaatggg taggcatggc gtcgagtatt tcattcgcaa tgataagacc
    12301 tgaaaattga tcaggtaatt gagctaacca agtaatgcga tcaaggaatt gcggtgcatg
    12361 agtctcaaat agcattcgct gacgctgctg taattcagcg cttatttcga gaatgagata
    12421 ttgttcaggt aattgattga attgttcaag ttccattaac aaatcgagcg ctaatttacc
    12481 tgatccagct ccaaattcaa gaatgctggt tttaccgatt tggtgaaata gctgcatcgc
    12541 ttgttttgcc agcgttcttc caaaaagagc cgatatttca ggcgcagtga caaaatcacc
    12601 gttaaatcca aatttaacgt tgcttgcact gtagtaaccc aaaccagggg tatataaagc
    12661 taattccata aattgggcaa aaggtatcca cccccctgat tcgtgaatct tatttacaat
    12721 ataactttgt aaatgttgac tatgctgcaa tccttcctga gtgggttggg gtagattgga
    12781 tgatggcatg aaagtaatcg tgtgtaagtg atgtgaaaga gttagagaat gatagcgagc
    12841 ggcgaaggat gaataaaggg ggaaatggat gacatcaaat aataaagtaa ttttgattac
    12901 tggcggagca aaacgtgtgg gcgcagccat atgtcgacaa ctgcatgcgc agggggccaa
    12961 tcttgccatt cattaccgtt cttctcaaga ggaggctgag gcactccgtg actcactgga
    13021 agcgatgcgt cgacagtcag ttttactaat cgaagctgat atactcaacg tagatcagtt
    13081 gccaaattta attgaacaaa taatactccg cttcggacag ctcgatgctg tagtgaataa
    13141 tgcatcgagt tttttttcaa caccgatcgg aagattttct gatgcagcat ggaatgattt
    13201 gatcggcagt aaccttaaag caccgctttt tctttctcaa gcagccgccc cttatttaat
    13261 caaacagaag ggttgtatta tcaatattgt ggatattcat gtagattatc ctttgaaaaa
    13321 ccatgtgatc tataattgcg cgaaaggcgg attagcagcg ttgactcgct ctttagcttt
    13381 agaattagcg cctgatgttc gcgtcaatgg ggtttcaccc ggtccgattc tatggccggt
    13441 aaccggtgat tgggcggata catcattaca gcatgaaata atcaatcgaa cattgttgaa
    13501 acgctgtggt gaaccggatg atatcgcaaa agcagttaat tacttgatta acgatgctcc
    13561 atttgtgact ggccaaatta ttgctgtaga tggcgggcgt agtattaatc tttaattcaa
    13621 tcattcatca aaagcgtttt taatccagtt gatttcatgc gttagctgtc cggtaattag
    13681 cacgacatca aaccgacaag gtggaatttt ttttaggttg gctaaataat gatttgcagt
    13741 cagtatgagt ttatgttgtt tcgattcagt aatactatga atagcacctc caaacgaagt
    13801 gtgagtgcgc attttgactt caataaaaac aagcattgtt tgatcgcgca tgatcaaatc
    13861 gatttcacca tagcgacaac gatagtttct ttcaacaagg actaaatgtt gttgttgcaa
    13921 ataatgtgca gctaaatctt cagcttcact accattcata ttaagtcaga aatgatccca
    13981 ttagcgtact tttgctttct aaagcaaacg agtgtgtgcg acaatttacc gatattaaac
    14041 agcgtcagta aacagagtca atgcagcaga ataacggtac attatatgta gtggcaacac
    14101 cgattggtaa tttgcgtgat attagcttac gtgcactgga tatatttgct ctagttgacc
    14161 ggattgcagc tgagcatgcg gatttgaccc gacatttatt gtcgcatcat ggtatcaaaa
    14221 cacctttgat gacactgcat caacataatg aacatttggc ggttgaaaaa atagctaatt
    14281 tgcttgcaaa taatcaagat gttgcattgg taacggatgc aggtacaccc tgtatttctg
    14341 atccaggcgc gatgttggtt catgcaatgc gtgctttagg acataaggtt gttccgattc
    14401 caggtgcaaa tgcagcaatt tgtgccttat ccgcatcggg tattcgatca acctctttct
    14461 ttttctatgg tttcctaccc gtaaaatcag gtgaacgcca acgctgcctg gcttcattaa
    14521 ctcaattact gtcttgtacg ctgatttttt atgaagcacc acatcgtatc gttgattgta
    14581 tcaacgatat gatcgctgtc ttcggcgcgc atcggcaaat tacccttggt agggagttaa
    14641 caaagatatt tgaaacgttt cataccgata atttagagaa taccttgcaa tggctattgg
    14701 cagatgaaaa tcaacaaaaa ggggaatttg tattgctatt accctctttt cttgaagaaa
    14761 atgagcctgg cctttccaga gatgcacagc atacggtaca agtattaatg aaggaattgc
    14821 cgttaaagca agcagtgcag cttgcggtat cgattacggg tgagaaaaag aaaagcctct
    14881 acgcgtatgc tttgtcgatt aagaatgatt aacatgactt gattagatta atctaaaaaa
    14941 tttatgtctc aaacgatcac gattatccaa ccggatgact ggcatctcca tttgcgcgat
    15001 ggcgagcaaa tgcagtgcgt attgcctgat tcagcgatcc ggtttgctcg agcggttatt
    15061 atgccaaacc ttaagccacc gattataacc actgatcaag caattcaata tcgctcgcgg
    15121 atcattgaat cattaccaaa tgcattgcgt agtcatttta atccactcat gacactttat
    15181 cttactgata atacttcccc ccaagaagta gcaaaggcta aaaagagtgg tatggtgcat
    15241 gccgtgaagc tttatccggc aggtgctaca acaaattcag atgcaggtgt taccgatctt
    15301 tcaaaatgct ttgctacgct agcgtctatg gagcaacttg ggctgccatt attagtgcat
    15361 ggtgaagtaa ccgatcctgc tgtggatatt tttgaccggg aatcggtgtt tattgatcgt
    15421 gtattaaccc ctttgattcg acgttttcca gtactacgca ttgttttaga gcatattaca
    15481 acacgcgaag cggttgaatt tatccgcagc acacctaacc acattgcagc aactatcacg
    15541 gcacatcatt tgttatttaa tcgtaatgca atttttacca gtggcattcg gccacattac
    15601 tactgtttac cgattttaaa gcgtgagaaa catcggcagt cacttgtcgc agcagcgatt
    15661 agtggttccc caaaattttt cttaggtacc gatagtgcac cgcattcaat cgcaaataaa
    15721 gaaagtaatt gtggctgtgc aggaatctat tctgcacatg cgggaatcga attttatgtt
    15781 gaagcgtttg atcgggctgg tgccctagac aaacttgaag cttttgcgag ttttcatggt
    15841 gcagattttt atcagctacc gagaaacgaa catcaaataa cgttgattaa gcaagattgg
    15901 ataatacctg cgcaaattcg ttttggtgat gaacaattga ttcccctaat ggcgggtgaa
    15961 aagctttcct ggaaactgag taatcatttt ttagcatggt aggccacgta aatcataata
    16021 agcacctaat ttatcagatt atttagcatt ttctgcggtg aactaattcc taatacagat
    16081 tgtcttttag ttgataggtc gtacacgatc gtttcctgaa aaaaggtata ctttcaaatt
    16141 ctgtttgaaa acgagtttag aaaaggatca atatcttaat gtttaggaaa caagaagtat
    16201 gagtgatcgc gaaatcgatc aacagctcgt ggagcgtgtt cagcaaggtg ataaaagggc
    16261 gtttgattta ttagtcgtca agtatcagcg caaattggct cgactattgt cacagctcat
    16321 tcgcgatcct gtagaagtcg aggatattgc ccaagagact tttattaaag cgtatcgtgc
    16381 actgccttca tttcgaggtg atagcgcttt ttatacttgg ctataccgaa ttggaattaa
    16441 tacagcaaaa aattttcttg tgtcacaagg tagaaaagta ccgacgattg aaggtaattt
    16501 tgataatgag gatgctgaaa attttgagga agcggctaaa tataaagaag ttagtacacc
    16561 cgaaagtgaa ctaatgagta aacaagttgc ccaaacggtt aataaaacgc tcgattccct
    16621 acccgaagag ctaaggactg cgattgtact acgggaaatt gaagggttaa gctatgaaga
    16681 tattgcatca atgatgggat gtccgatcgg aacagtgcgt tcacgtatct tccgcgcacg
    16741 tgaagcaatt gccgagaaat tacgtccctt actagggacg agtaaagata aaagatggtg
    16801 atggagagaa tatgatgaaa gaaaaaatgt ctgagctgat ggatggtgaa ctagataatc
    16861 gcaatataga ttcggtgttt acagaattta agagaaatga tgaactaatc aaggattggg
    16921 aaacatacca tatgattagt gatgcgatta aacgacctgc ttcgtttcaa tcatttgata
    16981 ttgctgataa agttagaaaa caactcaatc atgagcctat tatttttgtg ccgaatgcat
    17041 caaaagttgc gaagcataaa ctctataccc tttcggcagc tgcctcggta gcagttttag
    17101 taggtagctg gttgctgcta cagatagaaa atattcaacc cgttacaacc gttgctgaaa
    17161 aaaccaaaga aagaattcta gtttctcaat ctcctgcatc tcagccaacc ccaacattta
    17221 cttacacact tccatctttg gaataccatt attttcatca tcagcctcaa ccattagctc
    17281 gaaaagtttt tttacctaat ggcgcgatgt atggtccttt gacaagtgaa tatcaagtcc
    17341 cagaagctgg aaactcaaga tagtaaggat ctctgagtga acggcattaa aatatcaatg
    17401 ccgttacttt ttctttaaga tagaatctaa gtagatagat ttctagtcat tttatttata
    17461 ttttacagca ctactcttaa actaattagt aactttaact tgattcaaaa tagcagagat
    17521 ttatgcaaaa atttttactg agtttattgt ttgttacttc aattggaatt gtaaatgcaa
    17581 aagaattgcc tgatttcaca gggctcgttg agcaatatgg agcggcggtt gttaatatta
    17641 gtacaatcca aacgcagcag atatctgggg ggcagatttt ccctgatatt cctaatctac
    17701 ctgaagattc ccctttttat gatttttttc gcaggcatat gcaaccattt tctgcgccta
    17761 gaaaatttga atcgcgctcc ttgggttcag ggtttgttat cagtaaggat ggttatatct
    17821 taacgaatgc acatgtcgtt gaatcagcaa atgagattac agtaaaactt aatgataagc
    17881 gtgaatttaa cgcacaagta attggtactg atcgtaaaac ggatattgca ttgattaaaa
    17941 tcgatgcaaa agatttaccg gttgtgacgc aaggggatcc tgaaaatcta aaagtagggg
    18001 aatgggttat tgcgatcggt tctccgtttg gattcgaaaa tagtgtaaca gcgggtatcg
    18061 ttagtgcaaa ggggagatcg ctcgcgcaag aaaattatgt accttttatt caaaccgatg
    18121 ttgcaattaa tccgggtaat tcaggtggtc ccctttttaa tatgaaaggg gaagtcgttg
    18181 gtatcaactc acagatctat agtcgtacgg gtggttttat gggattgtct tttgctattc
    18241 caatcaatgt agcgaccgaa attgctgatc agttaaaaga acatggaaaa gtatcacgtg
    18301 ggaaaattgg cgttatgatt caggaagtta ctgaggagtt agctgaatca tttggtttat
    18361 cgaaagcgac cggcgcgttg gttgtatcga ttgaaaaagg agggccagcg gataaagcag
    18421 gaatagaagt tcgggacatt attctcgaat ttaatgggaa accaattgca aattctagtg
    18481 atcttcctcg tatcgttggt aatacaaagc caaaatcaga agtgacaacg caagtttggc
    18541 gtaaaggttc aactcagtcg cttacggttc ttgtcggtga aatgcctatt gaagataaga
    18601 gtgaacgcat tcaaaaacaa ggtaaagtac ctgataaagc caatcgactt ggccttgctt
    18661 taagcgaatt aacagataag ctaaaaaaac aactgggcgt ggagggtgga ctacttgttg
    18721 aagaagttca accgggaacg gctagtaggg ttggtatacg cgctggagat attattctcg
    18781 gattgaatag tcaagacatt aaaacagtta agcaatttaa ccaaatgcta gataaaatta
    18841 gcaaggggaa aaatattgcg ttattgatta aacgcggtga tatgaccaca tttattacca
    18901 tgaagcttga taattaatct taatttcaaa tttaaagaaa acatctgtgg atcaacctaa
    18961 tttaatcgtt tatgtaagag aagcatgcca tttatgtcag gaaatggttg cgctattaca
    19021 ccaatatcag cagaagactg catttaaatt ggaaatcatc gatatcgatg atgatcctcg
    19081 gttggtccag caatacaatg aacttattcc agtactaatc agtgcagata atcaacaaag
    19141 cgagatttgt catcatcacc ttgatattac tgcattagat gcttatttca ataaaattag
    19201 gtagaatcca cttcgctgat taatttcaac gagattgtgt aagtaagcat aaacatggta
    19261 aagcttactt acacgcatca acaacaactc aactaaaata agcttttttc ttcccggatg
    19321 cgaaacattc gtaatttttc cattattgcg catattgatc atggtaaatc cacattagcg
    19381 gaccgaatca ttcaatattg tggtggtctt tcggaacgtg aaatggaaga tcaagttctc
    19441 gattcgatgg atcttgagcg ggagcgtggc attaccatta aagcgcaaac agtagcgcta
    19501 aaatatgtgg gtcgtgatgg tcaggcttat ttattgaatt taattgatac accagggcat
    19561 gtcgattttt cttatgaagt atcccgatca ttggcagcgt gtgaaggggc cttgctcgtt
    19621 gtagatgctt cacagggtgt tgaagctcaa acagtagcga attgctacac ggcaatcgaa
    19681 caaggtgttg aggtcttgcc tgttttaaac aaaatcgatc tacctgcagc agatcctgat
    19741 cgagtcatat ctgagattga agatattatc ggtatcgatg ctaagcatgc tctaagggtg
    19801 agtgctaaaa cgggtgaagg cattcaagat atgcttgaag cgatcattga tcgaattcct
    19861 gaaccgcagg gagatagcag tgctcccctc aaagcgctca ttattgactc ttggtttgat
    19921 aattatgtag gcgttgttgt tctcgttcgc gtagtggatg gtgttcttaa acctggggac
    19981 aaaattatgc taatggcgac tcgagctaca caattatgtg agcaagtggg tgtattccta
    20041 cccaaagcag agcatcgtga tgcgctgagt gcaggagaag ttggttacgt tatttctgga
    20101 atcaaagaat taaaatcagc aaaagtaggg gatactataa cgctagtcga ttctccggcg
    20161 aatgaacctt taactggatt taaagaaatc aagccccagg tttttgcagg tctttatcct
    20221 gtcgaatcca atcaatatga agcattgcgg gctgcgctag agaaattgca attgaatgac
    20281 tcttcattgc agtatgaacc tgaaacatcc caagcattag gctttggatt tcgttgtggt
    20341 tttctaggct tactgcactt agacattgtg caagagcggt tagagcgtga atatgatatg
    20401 gacctcatta caacggctcc gactgttatt tatcaggtat tgatgcgcga tgggtcagag
    20461 atcgagatcg aaaatccttc caaaatgccg gatctttcaa aaattgcgga aattagagag
    20521 cctataatta caacgactat tcttgtacct gaagaatatg ttggcactgt aatgttacta
    20581 tgcaatggta agcgaggcgt tcagcggaat atgcagtaca tgggtaggca agtaatgctg
    20641 gtttatgaaa tgcctttaaa tgaagtagtt atggattttt ttgacaaatt gaaatccgtt
    20701 agccgaggtt acgcatctct tgattacgaa tttaaggaat ttcgtgcatc cgatttagta
    20761 aagcttgata tattgattaa tgctgaaaaa gttgatgcat tatcacttat agtccatcgc
    20821 tcaagtagtc agtatcgggg ccgcgagtta gcacaaaaaa tgcgtgaact cataccgcgt
    20881 caaatgtttg atattgctgt acaagcagca attggcgcac atgtcattgc tagagaaaat
    20941 atcaaagcgt tacgcaagaa tgtattagca aaatgttacg gtggggatat tacacggaaa
    21001 cgcaagctac ttgaaaaaca aaaagcgggt aagaaacgca tgaaacgtgt aggtaacgta
    21061 gaaatccctc aatcggcctt tttagcaatt ttacaggttg ataaatagct gattgccaca
    21121 aaatatgcga tttacaagtt aaaatatgcc aatattgaat aacgtgatta tcttgtaatg
    21181 cagtccgaat catcattttt tgattttaac gataaatgaa ctttccctta gtgcttctta
    21241 tcctcttggt cataacgggt acgatatggc tgattgat
//
LOCUS       DAOQLJ010000022        20615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_895_length_20615_cov_6.005253, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000022 DAOQLJ010000000
VERSION     DAOQLJ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 20615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20615
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_895_length_20615_cov_6.005253"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PK522_02960"
     CDS             complement(<1..374)
                     /locus_tag="PK522_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40727.1"
                     /translation="MATNTILGLGVGLFNAALGRNYLSELANAENSGMSLLQIADVLD
                     GIPQFTIDVMGGKTAEQQVDQFLSHYGLTSTSGAAYTTAKTFVNNMLSSGKGFGAVTV
                     AANDYLLGSGVAADFQPTATLL"
     gene            complement(445..633)
                     /locus_tag="PK522_02965"
     CDS             complement(445..633)
                     /locus_tag="PK522_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40728.1"
                     /translation="MLQINNKSRHDFPECLHTLDFLGRISQTCQLWRFLVATIVIVME
                     YAIRQTGTRKLTLNSLQG"
     gene            1031..1726
                     /locus_tag="PK522_02970"
     CDS             1031..1726
                     /locus_tag="PK522_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HNC40729.1"
                     /translation="MKPEDVAQYLQENPQFFETYADMLAEIKIPQPYDGRAISISERQ
                     ISTLREKNKLLQEKLHELINIGENNDTIGEKMHRLAVSLLEFNSVEELLHGLNYNLCE
                     DFSIPHVVMRLWYLNDIKSNQKRPEFSAVSKTVRISVESMRKPYCGPQVSDDIKPWFG
                     QDAKYLQSFSIIPLRRKRTIGLLVMGSPDAERFYPDMGTLYLERLGELVSSALARLER
                     IDLEQIIDEPPKI"
     gene            1707..2630
                     /gene="xerC"
                     /locus_tag="PK522_02975"
     CDS             1707..2630
                     /gene="xerC"
                     /locus_tag="PK522_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNC40730.1"
                     /translation="MSPPKFEEKPVSISAYLAYLRQVRRLAKLTYKSYERDLAVLNQF
                     SMSIPFHELEAHDIRHFIARLHGSGLSGKSLARTLSAWRTFYQYLIRFRGYQNNPCQG
                     IKVPKSPKHLPHTLSPDEAAKLMDFESTDSLSIRDRAMFELCYSSGLRLQELVQLKPH
                     DIDFTEAQVRVVGKGEKTRIVPVGDYALAALSAWLKQREALPKLDSDALFVSSRGNRI
                     HPRTVQQRLKIRADQQHLQSSIHPHVLRHSFASHVLQSSGDLRAVQEMLGHASISTTQ
                     VYTHLDFQHLAKIYDCAHPRAKRKKIAPSSD"
     gene            complement(2673..3686)
                     /locus_tag="PK522_02980"
     CDS             complement(2673..3686)
                     /locus_tag="PK522_02980"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNC40731.1"
                     /translation="MAIIKGWAALGPQQKLQPYTYEPGPLNGEEVEIAVEYCGLCHSD
                     LSMINNDWSLTRYPIIPGHEVVGRIIAIGDQVKGLKVGQSVGVGWNALSCMHCRECLT
                     GQHHLCPETQSTIVGRHGGFAERIRAHWVWVIPLPDTLDKSIAGPLLCGGITVFAPLQ
                     VFDVKPTDRVGVVGIGGLGHLALQFAHAWGCEVIALTSSPAKAEEAHKFGAYYSVSSR
                     DRSEISKLANTLDFLLVTVNVPLDWSSLLKTLKPNGRMHIVGAVLEPIPLQAFDLIMG
                     QKRISGSPTGGPTMTAMMLHFAARHRIAPQVEHFPLNQVNEAIAHLAAGKAKYRIVLD
                     HTN"
     gene            complement(3950..5203)
                     /locus_tag="PK522_02985"
     CDS             complement(3950..5203)
                     /locus_tag="PK522_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011113016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNC40732.1"
                     /translation="MKKKLVSLLVAGAMATPVITSAQVLNGVGGPGTHVTIFGRVQAE
                     YSTIDIDGKNAQTGILDDANQSRWGLHVSENLGMGLRAMGRIEYGLNPGSGRNDNARE
                     QWVGLGGDQWGELKFGRVQSPFKDFAGGHTIDPFAYTTLHANGSGGTMTASANGLGSG
                     DNSFVNSSARFDSAVVEGFSMSALLMPGDANRLNPQSFGGVGGYDPLNPNTGGEDGEW
                     DFQIAGKYSNKFDAHGIDIFGGYSRDNISDRMKAAGINDDEEIWRGGGKYSFADFTLR
                     GQYEYVNNANPLGGAATCSQAAALGSYGSLPSLLPSQTGDGGRGQCLSAMNANGDGYL
                     WFAGADYTIGNTKLVAQGGMAHAKAIGALDKRDVESLTVGVIHSLSRRSSLFGGYQRA
                     WVADRNATTTDSDRNVYTVGMRHDF"
     gene            complement(5514..7043)
                     /gene="recQ"
                     /locus_tag="PK522_02990"
     CDS             complement(5514..7043)
                     /gene="recQ"
                     /locus_tag="PK522_02990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNC40733.1"
                     /translation="MQSNQADTLLRTVFGHGDFRGQQSEIINHVIQGGDCLVLMPTGG
                     GKSLCYQIPAILREGVGIVISPLIALMQNQVEHLRQRGVRVAYLSSLLAQSEIEAVEQ
                     DLQANCYDLLYVAPERLLTPRFLGLLQKIKLSLFAVDEVHCISQWGHDFRPEYVQLSI
                     LQKKFPHVPRIALTASADMRTRTEIIEKLDLYKARIFISSFDRPNIRYRVVNKANERQ
                     QLLHFIKSEHPRDAGIIYCLTRKKVEDVAFWLSSKDVAALPYHAGLSAQVRKNHHQQF
                     LSDKGKVIVATIAFGLGIDKPDIRFVAHLDLPKSIEGYYQETGRAGRDGLPASAWMTY
                     GFGDVARLRHLIENTGTQAMVKQVAMEKLDALLTFCEAVTCRRIRLLNYFGESVPQVA
                     CGNCDLCLDPPQVWDGTVAAQKALSCVYRAGQHSDLDHWIDVLCGKYTEQVKRWGHDK
                     ISTFGIGKELNEKDWRKLFRQLMVLGYLTSSRNDIYSQRLTPASRAILRGEQTLYLRT
                     K"
     gene            complement(7088..7477)
                     /locus_tag="PK522_02995"
     CDS             complement(7088..7477)
                     /locus_tag="PK522_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNC40734.1"
                     /translation="MIEVTDCEKFRIDKWLFAARFFKTRALAAEAVERGRVLVNGVRV
                     KPARTVVVGNRLIIRMDQCQYEIEVLALSNRRGSYAEAQKLYQETDASRQLRAAIMEK
                     AKAEPPQFYTKGRPTKRDRRELDRFKG"
     gene            7894..9303
                     /gene="glnA"
                     /locus_tag="PK522_03000"
     CDS             7894..9303
                     /gene="glnA"
                     /locus_tag="PK522_03000"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNC40735.1"
                     /translation="MAVAEVLKKIQDHEVKFVDLRFTDTNGKEHHITVPAHTFTADKF
                     EDGHAFDGSSIAGWKGIHASDMLLMPDPETATLDPFMEETTLFLSCDVVDPSDGQGYN
                     RDPRSLAKRGEVYLQSTGIGDIAYFGPELEFFIFDSVRWQTTMSGCSLEIESEEAAWN
                     SKKKYEHGNTGHRPAIKGGYLPVPPVDSLHDIRSAMCVYLEEMGIDVEVHHHEVANAG
                     QCEIGTRFNSLVKRADWNQMLKYVVHNVANSYGKTATFMPKPIVGDNGSGMHVHQSIW
                     KDGKNLFSGNSYAGMSETALFYIGGIIKHTKALNAITNPGTNSYKRLVSGYEAPVNIA
                     YSASNRSAAIRIPHASNPKQRRIEIRFPDPLANPYLAFSALLMAGLDGIQNKIHPGDP
                     MDKNLYDLPPEEASKVPATCNALEEALEHLDKDREFLTRGGVFSNDMIDAYIALKMEE
                     VILYRTAPHPIEFSMYYSL"
     gene            9433..9891
                     /locus_tag="PK522_03005"
     CDS             9433..9891
                     /locus_tag="PK522_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40736.1"
                     /translation="MKQIISITLILLAIFTITAQVSASSNIHKTVDNKGRVTYANAQG
                     KNYAKATHASLSSGRIYPKIDNHQQKKRDFDRRQILENELAIETELLVGIINSLRLIS
                     IKMKEIQSESSQPVSAAINDFENDNINTLLNLASVHERNIIALRKELNSL"
     gene            9910..10359
                     /locus_tag="PK522_03010"
     CDS             9910..10359
                     /locus_tag="PK522_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40737.1"
                     /translation="MKTVLLFAILFAHSICFAEPSIYKHFGDRGETIYSDHHLKGAEQ
                     AELPVLTVLPTSQRLSKVSPSIAQMEADESLQHNETEQIGTHQSRVIPSAKSEKVMQP
                     TEISANQTDEVELSPEEQLQLIIDDMELYDDDIDRLELELYKIGADY"
     gene            complement(10414..11184)
                     /gene="xth"
                     /locus_tag="PK522_03015"
     CDS             complement(10414..11184)
                     /gene="xth"
                     /locus_tag="PK522_03015"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNC40738.1"
                     /translation="MKIGTWNVNSLKVRLPQVIQWLEVNQPDVLCLQETKLQDSFFPL
                     TELSASGYSSIYVGQKTYNGVALLSKQPGCDVITTIPNFEDDQKRVVAATYGDIRVVC
                     IYVPNGESIDSEKFQYKLNWLSALNRWLHEELTQFPKLVLLGDFNIAPADSDVHNPEL
                     WKDQVLCSSPERDAFQNLLNLGLYDSFRLFEQPGKSYTWWDYRMLAFRLNKGLRIDHI
                     LLSKALTESCESCVIDKTPRKLERPSDHTPVVVTLKIG"
     gene            11419..12246
                     /locus_tag="PK522_03020"
     CDS             11419..12246
                     /locus_tag="PK522_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC40739.1"
                     /translation="MNPHVAPPTSPFYLLKSLRAHGSLIFNLIKREIIGRYRGSIMGI
                     LWSFFNPVLMLIVYTFVFSIVFKARWIGGTDSKTEFALVLFAGLMMYNLFAECINRAP
                     GLVLGNVNYVKKVVFPLEILPIVAIGSATFHLIISLFVWLCFYLIFIGIPPSTIGLLP
                     VLLFPFFLMILGLTWLLASLGVYLRDVTQIIGILTTALLFLSPIFYPISALPEEYHYF
                     IQVSPLTFIVEQAREIMIWGKELNWSAWIIYSLFSIVTAWLGFAWFQKTRKGFADVL"
     gene            12236..13606
                     /locus_tag="PK522_03025"
     CDS             12236..13606
                     /locus_tag="PK522_03025"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC40740.1"
                     /translation="MSSEIALQTDGLGKCFHIYDKPRDRLLQMFGASKKKFFREFWAL
                     RDISFTISKGEIIGVIGTNGSGKSTLLQIIAGTLSPTIGTVNVIGRVSAILELGAGFN
                     TEFTGRENVLLNAAIIGFSRDAIAEQMEKIRAFSELGDFFDQPIKTYSSGMQARLAFS
                     IAIHVDPEILIVDEALAVGDARFVAKCMRRIKDIMSKGTTVLFVSHDISSVRTLCSRT
                     LWLDQGYLVEDGDVFPVTGRYMAYMFNNDQPSIEASNLAHKNIARHKTKSTKLSELPI
                     ENQADSSLQGEITGNGQTSPPDSRPEIALDSKPITHWGSYKGIILSASVRDSHGVRKN
                     TLHWGAPIEIFIDLRIPPEISRDHLSIAFSIKDLKGTDLIVSTTHDFELKRLPEDERF
                     SISFKLTNPLVTGKYLLVAAVENRQYRDIHYYEYFEGAHYFASLSKERFFGLFQPSIE
                     QAVSLK"
     gene            13608..16724
                     /locus_tag="PK522_03030"
     CDS             13608..16724
                     /locus_tag="PK522_03030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC40741.1"
                     /translation="MLMDSINSNAYWNNRFKSNWDSFEGPAQSRFFSQIAIEHLPKWF
                     IEQIKRNNLTFADWGCAQGDGSDVWASYIDSSQITGIDFSLAAIEKATRRYPAICFLN
                     EDWLSKSLISNEIVYDIIFSSNTLEHFHKPYEVLTSILKRARKAVVLALPYKEIDRID
                     EHFFSFLPENIPLMLKNNFRLVVSRVIDCKTRPRTAWQGYQIILLYAHSDWIDQLLLS
                     LNDSRIESFDADSHIENLNNQIKRLNDEITHQTEQIVQLKQTILEQNQHITHYQQTLS
                     EYNLKQQQFYQVIAERDTHISNLEQTLTQRDDQINQLEIKRLLEYEKTIQLSDWAQKI
                     DNRPFHYGTKKLVLKSAKNFYHALPISHRYKQKFKFFLSQIPYLKKIIKTFPVSVNQI
                     SRSALDHKPLVPDNLRALINGEKLNSRSDYLIFSVIDWHFRLQRPQHLARSLAKLGKR
                     VFYFSNHFIDSPTPGYAIERLDSDLELYQIKLYVEDAPAIYFSSPTVKHLNNLQLSIT
                     NFFIDFATLSSVSIIQHAYWYPLAIGLPNSLRIYDCMDHHEGFGNVSEDLIKLEKEMM
                     CKFDQIIVSSAWLEKQAQQYNNNVVIIRNASEYEHFNNPPIEIYLDPKGRKIIGYYGA
                     IAEWFDLELVKNLALAEPNALILLIGNDTINASHISKAIPNIIFIGEVSYNRLPFYLY
                     AFDVCLLPFKISPLTLATNPVKIYEYLASGKPVVSVDLPEISQFENLVHRAKTSDEFI
                     SLVSTCLKSSSPEENQTELRKQFACKQTWNHRVNDLVQAIQQIPMPKVSVIVLTYNNL
                     NLTKLCLNSLLQWSDYPNLEIIIVDNNSSDNTPDYLTELNTRHPTIQVIRNKTNEGFA
                     KGNNIGLIAATGDYLVMLNNDTVVTPGWILTMLRHLQADPSIGIIGAVTNNIGNEAKI
                     DITYPLLSEEMLPRILSHTIPHMGKNFKLRTAAFFCVMMSRNVFQQVGLLDENYGRGF
                     FEDDDYCRRIDRLDLQIACAEDVFIHHHLSASFNQIPDRDKQALFEKNKAYYESKWGK
                     WNPHEYRH"
     gene            16708..17817
                     /gene="wecB"
                     /locus_tag="PK522_03035"
     CDS             16708..17817
                     /gene="wecB"
                     /locus_tag="PK522_03035"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNC40742.1"
                     /translation="MSIGINRTILCVIGTRPEAIKMAPVILELRKKSFQVRVLATAQH
                     RNLLDQVLDLFGIVPNIDLNIMRPNQSLAPLTARLLLSAEEVLASEKPDAVLVQGDTT
                     TVMAVALACFYQHIPIGHVEAGLRTWDMDNPFPEEANRVITGKLARWHFAPTEESRLN
                     LMRDGISDNNIFITGNTVIDALLSLADQNLALPINLDPDKRLVLITAHRRENFGEPLR
                     SICQAIKSLAQNNPGVEFLYPVHPNPNVTETVNELLASQKNIHLCAPLDYAHFVAVMK
                     RSFLILTDSGGIQEEAPALSKPVLVLRNETERPEAVTQGVAKLVGHDYDRILSETQRL
                     LDDELAYFAMRKGISPYGDGHAAERIVKVLEERLP"
     gene            17817..18950
                     /locus_tag="PK522_03040"
     CDS             17817..18950
                     /locus_tag="PK522_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNC40743.1"
                     /translation="MQLVYLSPLPWDSFSQRPHQFVEWFHNKHGGEVLWIDPYPTRLP
                     NLGDFRRIKSSKFLSKNVIVGNGNKPPWLTVIQPFVPPIEPLPGSCYVYRWLWNDILQ
                     AIDRFIESQNETLLGIGKPSELALQVLFRYPSLKSIYDAMDDFPAFYHGISRFAMMNR
                     EQRIAKQVTRISASSTKLTERFIEYQSKLTLVHNACAVDALPSINDTLKTRATEKQVL
                     GYLGTIGDWFDWAFVAKLAETVSTSTIHLIGPIFTKIPASLPQNIKFLPACEHSAAIQ
                     AMLSFTVGLIPFKRNDLTAAIDPIKYYEYRSLGIPILSTHFGEMSLRQGQPGIFIANA
                     SCDLGDHINKAMDYKYPKSEINWFRKINSWEARFNSGNILPQT"
     gene            complement(18975..20222)
                     /locus_tag="PK522_03045"
     CDS             complement(18975..20222)
                     /locus_tag="PK522_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HNC40744.1"
                     /translation="MSLKEKLSQFGPGLLYAGAAVGVSHLVQSTRAGAEFGFQLVAIV
                     ILVNILKYPIFELGPRYAAATGKTLLDGYHQIGAWAVWIYFLLSALTMFIVMGAISIV
                     TAGLFAQLTGMPLDAPIIAMLLLVLSVQILGFGHYSLLDRSMKFIMIVLTISTLLALI
                     FSFFAPIEKQAEFETTFNFLESAHILFLVALIGWMPAPIDISIWHSVWSVSKNKEQGH
                     RIPLAQALLDFKVGYWGTMVLAVCFLTLGALVMYGTPESPASNSTEFAKQFIGFYTTS
                     LGEWAFPIIALAAFATMFSTLLTCLDAYPRTLRRSTELLFPRLDSLPYHNKIYWYWLI
                     LTALGTIVLLHFFLRDMRAMVDLATTVSFVLAPVLAILNYMALKRSEVPAHAKLTGAS
                     QYAYLASTGMLIAFSLWFLAYKP"
BASE COUNT         6226 a         4659 c         3910 g         5820 t
ORIGIN      
        1 ttcagcaatg ttgcggtcgg ttgaaaatca gcggccacac cgctgccgag caaatagtca
       61 ttggctgcaa cggtaactgc accaaaccct ttaccggaag acagcatgtt gttaacgaaa
      121 gtttttgcgg ttgtatacgc tgcaccagaa gtactcgtca aaccataatg actcagaaac
      181 tgatccactt gctgctcagc ggtttttccg cccatgacat cgattgtaaa ttgtgggatt
      241 ccatccaata catcggcaat ttgcagcaag ctcatgccgc tgttctcggc attcgccaat
      301 tccgacaaat agttcctgcc cagagctgca ttgaataaac caactcccag cccaagaata
      361 gtattagtag ccataaaaag tgcttccttt taagtagagt taaaaaaact tcagacaaaa
      421 accacagtca cgaaataaaa caatttatcc ttgcaagctg ttaagcgtca gttttcgcgt
      481 tcctgtttgc ctgattgcat attccatgac aatgacaatg gttgccacca gaaatcgcca
      541 cagttgacaa gtttgggaga ttctgcctaa aaaatccagt gtatgcaagc actctggaaa
      601 atcatgcctt gatttgttgt taatttgcaa catttatcct ccccggtaat gttaaaaata
      661 caactcaact atttttcacc atcccaatct tcacccaaag ccaattaaca cctgaatcta
      721 gtctttgagt gagatcttca tttgtgcacc acagaatctt cattttacta taactgatcg
      781 atctaaaatt tactgtgata tcgatcatat tctaaatcgc tgcccaatgc tcaatcgtat
      841 actttgatga gaactaagtg cgcagcattc tatcaacaat aaattcaagc catctggtca
      901 atatacgcta cctttaccac tgttttcagt gtatatatta cttctaatta atcccaaaca
      961 cttacccagc ttagcatttt atgcgctaat atcatatgct attgttatcg aatcgtgtag
     1021 gaaatgatct atgaagccag aagatgttgc acagtattta caggaaaatc cccaattctt
     1081 tgaaacgtat gctgatatgc tcgctgaaat taaaattccg caaccttacg atgggcgcgc
     1141 catttcaatc agtgagcgtc aaataagcac tttacgggag aagaataaat tactgcaaga
     1201 aaagctgcat gaattaatca atattggaga aaacaacgat acgatcggcg aaaaaatgca
     1261 tcgcttggct gtttcattgc ttgaatttaa tagcgtagaa gaactattgc atggcttgaa
     1321 ttacaatctt tgcgaggact tttcgattcc ccatgtcgtc atgcgcttat ggtacctcaa
     1381 tgatattaaa agcaatcaaa aacgccctga attcagcgct gtgagtaaaa cagtgagaat
     1441 ttctgtggag agcatgcgta agccttattg tggcccccaa gtcagcgatg acatcaaacc
     1501 gtggtttggc caagatgcaa aatatctgca atctttctct attattccgc ttcgtcgcaa
     1561 aagaaccatc ggtttgttag tgatgggcag ccctgacgct gagcggtttt accccgacat
     1621 gggcacgctt tacctggagc gactcggtga attggttagt agcgccctcg cacgactcga
     1681 gcgcattgat cttgaacaga taattgatga gccccccaaa atttgaagaa aaaccggtaa
     1741 gcattagtgc ttaccttgcg tatttacgcc aagtccgtcg gttagcaaag ctaacttaca
     1801 aaagctatga acgcgattta gcagtactca atcaattttc gatgagcatc cccttccatg
     1861 aattagaggc acatgatatt cgccatttca tagcccgatt acatggctca gggctatctg
     1921 gcaaaagcct tgctagaaca ttatcagctt ggagaacttt ttatcaatac ttgattcgat
     1981 ttcgtggtta tcaaaataac ccgtgccaag gcatcaaagt gcccaagtca cccaagcatc
     2041 tacctcacac attatcaccg gatgaagcag ctaaattaat ggatttcgag tctaccgata
     2101 gcctcagcat acgcgatcgt gccatgtttg aattgtgcta ttcatctgga ttacgcttac
     2161 aagagctagt ccaactcaaa ccgcatgata tcgattttac tgaggcacaa gttcgcgttg
     2221 ttggcaaagg cgagaaaacc cgcatcgtac cggtaggtga ttatgccttg gcggcattaa
     2281 gcgcctggct gaaacagcgc gaagctttac cgaagctcga tagtgatgcg ttatttgtct
     2341 cgagtcgcgg taatcgaatt cacccacgaa ctgtccagca gcgcctcaaa atacgcgccg
     2401 atcagcagca cttacaaagt agtatccatc ctcacgtttt acgtcattct tttgcgtctc
     2461 atgtattgca atcgagtgga gatctacgcg ccgtccaaga aatgcttggg catgccagca
     2521 taagcacgac tcaggtctat acacacctcg attttcaaca tttagcaaaa atctatgact
     2581 gcgctcaccc acgcgccaag cgaaaaaaga ttgctccatc atcagactaa ggcccagcag
     2641 ttacccaacg aagcgcttga gattactaga agctaatttg tgtgatctaa cacaatccga
     2701 tattttgctt tcccagcagc aagatgggct atcgcctcgt tgacttgatt cagtggaaaa
     2761 tgttctactt gcggcgcaat gcgatgtcga gctgcgaaat gcagcatcat tgcagtcatt
     2821 gttggtccac ccgtgggtga tccggaaata cgcttctgcc ccataattaa atcgaatgct
     2881 tgaagaggaa tgggttcaag cacagcccct acgatatgca ttcgcccgtt tggcttaagc
     2941 gttttaagca atgatgacca atccaaagga acattcacag taaccaataa gaaatccaat
     3001 gtattagcta gtttactgat ttcactccga tcacgactgg atacactata gtatgcaccg
     3061 aacttatgag cttcttcagc ttttgcagga ctcgaagtca atgcaatcac ctcacatccc
     3121 caggcatgcg caaactgtag tgctaaatgc cctaacccgc caattccaac gacaccgact
     3181 cgatctgttg gcttcacatc aaaaacctgt agtggagcaa ataccgttat gccgccacag
     3241 agcagtggcc ctgctatgga cttatctaac gtatcaggca atggaattac ccaaacccaa
     3301 tgcgcccgaa tccgctcagc aaaaccccca tgacgcccta caatcgttga ctgagtctct
     3361 gggcaaagat ggtgctgacc tgttaagcat tcccggcaat gcatacaact cagtgcattc
     3421 cagccaaccc caaccgattg accaactttt agtcctttta cttgatctcc tatcgcaata
     3481 atacgcccta ccacttcatg cccaggaata atcggatacc gagtcaagct ccagtcattg
     3541 ttaatcatcg acaaatcaga gtgacaaagc ccgcagtact cgacagcaat ttctacctct
     3601 tcgccattca aaggacctgg ctcgtaagtg taaggttgca acttctgctg aggccctaat
     3661 gcagcccatc ctttgattat ggccaaaatt ttctccttga taattaaagc atgtgtaaga
     3721 gaaataataa gactgattct ctacttaaca caagcgcacg atttaaccag ttttcgcccc
     3781 taagattagg tttagcgaaa ttaaggtaat gtatcgttca aattagaaac aaagcaagga
     3841 ttcaatttca gtcattcatt aagtaaaccc tgagtacttt aacttacgaa agttgcaata
     3901 aaaaaggggt gccttttggg cacccctttc ttttcaaact tttctaatct tagaaatcgt
     3961 gacgcatacc cactgtgtaa acgttacggt ctgaatcagt cgttgttgca tttctatcag
     4021 caacccaagc acgttgataa ccaccgaaca agcttgaacg tctgcttaaa ctatgaatca
     4081 caccaaccgt tagcgactca acatcacgtt tatcaagcgc accgatcgct ttagcatgag
     4141 ccataccgcc ttgtgctacg agctttgtgt taccgattgt gtaatcagca ccagcaaacc
     4201 agagataacc gtcaccattt gcgttcatcg ctgataaaca ttgaccacgt ccaccatcac
     4261 ccgtttgtga aggcaataag cttggcaatg aaccatagct tcctaaggct gctgcttgtg
     4321 aacaggtcgc tgcgccgccg agtgggttag cattgtttac gtattcatat tgcccacgta
     4381 aggtaaaatc agcaaaactg tatttaccac caccacgcca gatttcttca tcatcattga
     4441 taccagccgc tttcatacga tcactgatgt tgtcacggga ataaccaccg aagatatcga
     4501 ttccatgagc atcaaatttg ttgctgtatt taccagcaat ttggaagtcc cattcaccat
     4561 cttcaccacc ggtattagga ttcaaaggat cgtagccacc gacaccccca aagctttgtg
     4621 gattgagtct atttgcatca cctggcatta acaaagctga cattgagaaa ccttcaacaa
     4681 ccgcagaatc aaaacgcgct gaactgttaa caaaactgtt gtcaccagat cctaagccat
     4741 ttgcactggc tgtcatggta ccaccactac cattagcgtg tagggttgta taagcaaacg
     4801 gatcaatagt atgaccaccg gcaaaatctt taaatggaga ttgaacacgc ccaaatttca
     4861 attcgcccca ttgatcgccg cccagtccta cccattgttc acgggcatta tcatttctac
     4921 ctgaacctgg attaagaccg tattcgatac gacccatcgc tcttaaaccc ataccaagat
     4981 tttcactgac atgcaaaccc cagcgtgatt ggttagcatc atccagaatg cctgtctgtg
     5041 catttttgcc atcgatatca atcgtgctat attcagcttg tacacgacca aatatcgtga
     5101 catgggtacc aggtccacca actccattca gtacctgtgc tgaagtaata actggggtcg
     5161 ccattgcacc cgcaactaac agtgaaacta gtttcttttt catctgaaac tctcctataa
     5221 aattagaacg actattcgcc atgcttaaaa tctttcgctt ccattctcgc tgaatgtttt
     5281 caattatcca gacctttcga actggaaagg ttaggcagat tgtgcacaac acaagataat
     5341 gatgcaacaa aaaacaacta acttttctgt ttttaacaac atttgttgtt tttgaacaac
     5401 atttgtgggc gccaaacgac ttaatttacg aaaataaaac caaaaaatac gctaattcaa
     5461 tgctttaaaa ataatgaata cgtaaaaaaa cgataatttc aaataaaagt cagttacttt
     5521 gtacgaagat agagcgtttg ttctcctcga agaatggcac gactcgctgg cgttagtcgc
     5581 tgcgagtaaa tatcattgcg acttgacgtc agataaccga gcaccataag ctgccggaac
     5641 aatttccgcc aatctttttc atttaattcc ttaccgatac caaacgtact tattttatcg
     5701 tgtccccagc gcttaacctg ttctgtatac ttgccgcaca acacgtcgat ccaatgatcg
     5761 agatccgaat gctgcccggc acgatatacg catgacaacg ctttctgcgc agcgactgta
     5821 ccatcccaaa cttgcggtgg atcaaggcaa agatcgcaat taccacaagc cacttgcggt
     5881 acagattctc caaaatagtt gagcaatcgt atccgacgac acgttactgc ttcacaaaat
     5941 gtcagcaaag catctaattt ctccatcgca acttgtttaa ccattgcttg cgtacccgta
     6001 ttttcgatca aatgccttaa tcgagcgaca tcaccaaatc cataggtcat ccaggcgctg
     6061 gctggcaaac cgtcacgacc agccctaccg gtttcttggt aatacccttc aatactttta
     6121 ggcagatcca gatgcgcaac aaaacgtata tctggtttgt caatcccaag gccaaatgca
     6181 atggtcgcaa cgatgacctt ccctttatca ctcaaaaatt gctgatgatg attttttctt
     6241 acttgagcac tcaatcctgc atgatagggt aatgcggcta catctttgct cgataaccaa
     6301 aaggcaacat cttcaacttt tttgcgggtc aaacaataaa taataccggc atcacgagga
     6361 tgttccgatt taatgaaatg gagtagctgc tgccgctcat tagctttatt gacgactcga
     6421 taacgaatat taggtcgatc aaaactcgaa ataaagatgc gcgctttata caaatccagt
     6481 ttttcgataa tttcagtacg cgtcctcata tcggcagatg ctgttagcgc tattcgaggc
     6541 acatgcggaa attttttctg caaaatcgaa agttgaacat attcgggcct aaaatcatgc
     6601 ccccattgag aaatacagtg cacttcatca acggcaaaca gcgacagctt aattttttgc
     6661 aataacccca gaaaacgggg tgtaagcaat ctttcgggag caacataaag caaatcatag
     6721 caattagctt gcaaatcctg ctcaaccgct tcaatttccg attgagcaag caaggaactc
     6781 aaatacgcca cacgaactcc tcgttgcctc aaatgctcga cttggttttg catcaacgca
     6841 atcaaaggtg agatgactat tccaacacct tcacgcaata ttgcaggaat ttgataacac
     6901 aaggatttac ccccgcctgt cggcatcaat acgaggcaat cccctccttg aataacgtga
     6961 ttaatgattt ctgattgctg tcctcgaaaa tcaccgtgcc caaacactgt tcttaggaga
     7021 gtgtcagcct ggttcgattg cacgcttgat cgtcctgttt atagttttct tatgatattt
     7081 gaccaatcta gcccttgaat cgatctaatt ctcgtctatc gcgtttggtt ggcctacctt
     7141 ttgtataaaa ctgaggtggc tcagctttag ccttttccat aatggctgca cgtaattgtc
     7201 gacttgcatc cgtttcttga taaagcttct gagcctcagc ataggaacct cgtcgattgc
     7261 tcaacgctaa tacttcaatt tcatactgac actgatccat acgaataatc aatcgattac
     7321 cgactacaac cgttcttgct ggtttgactc gtacaccatt gacaagcacc ctacccctct
     7381 ctaccgcttc agccgctaat gcgcgggttt taaaaaaacg ggcagcaaag agccatttat
     7441 caatacgaaa cttttcacaa tcggttactt cgatcatctc aatccaattt catatttatc
     7501 tgcgtggaag ctcctgagta gattcaatta ttggtgccag atcctatccc tatgctattt
     7561 agcgcactat atccttgtag tatcgataac agcgagggca ataaattaca aattttgtaa
     7621 aagatgattt tagtcattaa taatcaataa gaacagtaag tccagattct tatataatta
     7681 gaagctaaat tcaaatttta agagggtttg tagtttgact tttatttact ttaatactcg
     7741 attaatcacg taaatacttg tttaacccga cacatcagag ctaattaatt agctatattt
     7801 aaaatggttt tttgaatatt cagcactata tcaattaaca aatcattaat tcaccatcta
     7861 aaaagaatca tcattctgtc tggagaaaat taaatggcgg tagcagaagt tttaaaaaag
     7921 attcaagacc acgaagtaaa atttgttgat cttcgcttca ccgatacgaa cggtaaagag
     7981 catcatatta ctgtccccgc tcatactttt actgcagata aatttgaaga tggtcatgcg
     8041 tttgatggat cctcaatagc cggttggaag ggtattcatg cttcagatat gctgcttatg
     8101 cctgaccctg aaactgctac tttagatccc ttcatggagg aaacaacgct ctttcttagc
     8161 tgcgatgttg ttgatccttc agatgggcaa ggctataacc gtgatccacg ctccttagca
     8221 aagcggggtg aagtctacct ccagtcgact ggcattgggg atattgccta ttttggtcct
     8281 gaactggaat tcttcatttt tgattctgtt cgctggcaaa ctacaatgtc aggctgctcc
     8341 ttagaaattg aatcggaaga agctgcctgg aattcaaaga aaaaatatga gcatggtaat
     8401 actggacacc gccctgctat caagggtggt tatttaccgg ttccccctgt cgattctctg
     8461 catgatatcc gttctgcaat gtgcgtatac cttgaagaga tgggaatcga cgttgaggtg
     8521 catcatcacg aagttgcaaa tgctggtcaa tgtgagattg gaacacgttt caatagtctc
     8581 gtgaaacgtg ctgattggaa ccaaatgctc aaatatgtgg tgcacaatgt ggctaattct
     8641 tacggcaaaa cagccacgtt tatgcctaaa ccgattgtag gagataatgg ttcgggaatg
     8701 cacgtgcacc aatctatctg gaaggatggt aaaaatttat tttcaggaaa tagctatgcg
     8761 ggcatgtctg aaacggccct attctatatt ggcgggatca ttaagcatac caaggcatta
     8821 aatgcgatta ccaatccggg aacaaattcc tataaacgac tcgtttctgg ctatgaagcg
     8881 cctgttaata tcgcttattc cgcaagcaac cgctctgcag caataagaat tccccatgcc
     8941 agcaacccca aacaacgccg tatagaaatt cgttttcctg atccactcgc caacccttat
     9001 cttgcatttt cagcattact gatggctgga ctagatggta ttcagaataa gattcacccg
     9061 ggcgatccca tggataagaa tctatatgac ctaccgccag aagaagcatc caaagttcca
     9121 gcgacctgca atgctttgga agaagcgtta gaacatcttg ataaagatcg tgaattcctt
     9181 acgcggggag gcgtgttttc taatgatatg atcgatgcgt atatcgctct aaaaatggag
     9241 gaagtcattc tctatcgaac agcaccccac cccatagaat tttcgatgta ctacagctta
     9301 tagataaagg gttgatttaa agcgtttatt gtttgtttgc ttgagtcctt tttgattgaa
     9361 aatagaggaa atatcaagat acgccacttg atatttttct cgctgttagc tatttcaatt
     9421 atggaggtat tcatgaaaca aattatctca attacgctaa ttctcttagc gatatttacc
     9481 atcacggccc aagtgagtgc atcatccaat atccataaaa cagtggacaa taaaggacgt
     9541 gtaacttacg ccaatgccca agggaaaaat tacgcaaaag caacgcatgc gtcgctatca
     9601 agtggtcgga tttatcctaa gatagacaat catcaacaaa agaaacgtga ctttgatcgc
     9661 cgtcagattt tggaaaatga actggctatc gaaacagagt tactggtagg catcattaac
     9721 tcgctaaggt tgatttcaat aaaaatgaaa gaaattcaat cggaaagttc gcaacctgtg
     9781 tctgctgcca tcaatgattt tgaaaatgat aatatcaata cacttttgaa tttagcttca
     9841 gtgcatgaaa gaaacattat cgccttgcgc aaagagctaa atagcctttg atatttgtac
     9901 ccgagtatga tgaaaacagt cttgttattt gcaatcttat ttgctcacag tatttgcttt
     9961 gctgagccaa gcatctataa acacttcggt gataggggcg aaacgattta ctccgatcat
    10021 cacctgaagg gggcagagca agcagaattg ccagtgctga ctgttttacc tacttcccaa
    10081 aggctatcta aggtatcgcc ctcgatagcc caaatggaag cagatgagtc attgcaacat
    10141 aatgaaacag aacaaattgg cacgcaccaa tccagagtta tcccatcagc taaatcagaa
    10201 aaggtaatgc agcccactga gataagtgca aatcagactg atgaggttga actcagccct
    10261 gaagaacaat tacagcttat tattgatgat atggaattat atgatgatga tatcgatcgg
    10321 cttgagctag aactctataa gattggtgct gattattagc ctgactagtg gctgcgcact
    10381 tctagtaatc gttcagaaaa accacatttg aatttaacca atttttagcg tcacaactac
    10441 cggcgtgtga tcagaaggtc tttctaattt cctgggtgtt ttatcaatga cgcaggattc
    10501 gcaactttca gttaacgctt tacttaacaa gatatgatcg attcttaacc ctttatttag
    10561 tctaaacgca agcatccgat aatcccacca cgtataagat ttcccaggtt gttcaaacaa
    10621 tcgaaaacta tcgtataaac cgagatttaa aagattctga aaagcatccc tttcaggaga
    10681 gctgcataat acttgatctt tccatagctc aggattatgt acatcgctat ctgcaggtgc
    10741 aatattgaaa tctccgagta aaaccaattt tggaaattgt gttaattcct cgtgcaacca
    10801 gcgattaagc gctgataacc aatttaactt atattgaaat ttctctgaat cgatactttc
    10861 accattggga acataaatac aaacgacccg aatatcacca taagttgctg caacaactcg
    10921 cttttgatca tcctcaaaat ttggaatcgt tgtaatgaca tcacaacctg gttgcttact
    10981 aagcaatgcc accccattat aggttttctg gccgacataa attgatgaat acccactggc
    11041 agataattct gtcagcggaa agaagctatc ttgtaatttg gtttcttgca aacataaaac
    11101 atcaggttga ttaacctcta gccattgaat aacctgaggt agacgaactt ttaatgaatt
    11161 aacgttccag gtaccaattt tcatcctgaa aaaaataggt tatgtgcttt attttaaaat
    11221 ttggtggtct tgcaactcat tccattttag cagcaactcc attcgaattg ctgggataga
    11281 agcgggcatg ctaccattac ataaatgcct tgaccagttt gaactcaaaa aaaatcgtgt
    11341 ttgtcattaa ctcaacccgt aataacaata gtgcatttta gcctattaag tattcgcata
    11401 catttaactt tctaaaaatt gaatccacac gtagcgccgc ccacttcccc cttttattta
    11461 ctgaaatcac taagggctca cgggagtctt atttttaatc taataaaacg tgagatcatt
    11521 ggacgctatc gaggctcgat tatgggaata ttgtggtcat tctttaatcc cgtactcatg
    11581 ctaatcgttt acacatttgt tttcagtatc gtatttaaag cacgctggat cgggggcact
    11641 gactccaaga ccgaatttgc tttagttctt tttgctgggc taatgatgta caacctattt
    11701 gcagaatgta tcaacagagc ccccggtctt gtgcttggca atgttaatta cgttaaaaaa
    11761 gtagtatttc ccttagaaat attacctatt gtcgcgatag gctcagcaac gtttcatctt
    11821 attatcagcc tgttcgtttg gttatgtttt tacttgattt ttattggaat tccccccagc
    11881 acgataggat tgttaccagt cttgttgttc ccattctttc tgatgatttt aggattaacc
    11941 tggctcctag catcgctcgg tgtttattta cgggatgtca ctcaaatcat tggaatttta
    12001 acaacagcat tgctgtttct ctcacccatt ttctatccaa tttctgcttt acccgaggaa
    12061 tatcattact tcattcaagt tagcccgcta acctttatcg ttgaacaggc acgtgaaata
    12121 atgatttggg gtaaagaact taattggagt gcttggatta tttactcatt attctcaatc
    12181 gttaccgctt ggcttggctt cgcctggttc caaaaaacgc gtaaaggttt tgccgatgtc
    12241 ctctgaaatt gctttacaaa ctgatgggtt aggtaaatgt tttcacattt acgataaacc
    12301 tagagataga ttgttacaaa tgtttggcgc gagtaaaaaa aaattcttta gagaattctg
    12361 ggcattacgt gatatatcct tcacaatctc taaaggagag atcatcggag taattggaac
    12421 caatggcagt ggaaaatcaa ctttactgca aatcattgca ggaacactct ctccaacgat
    12481 cggcacagtc aatgtgattg ggcgtgtatc agccattctt gaactcggcg caggcttcaa
    12541 tactgaattt accggccgtg agaacgtact gctcaatgcc gccattatag gtttctctag
    12601 ggatgcaatc gcagaacaaa tggaaaaaat ccgtgcattc tctgagctcg gagacttttt
    12661 tgatcaaccc attaaaacct actccagcgg aatgcaggca cgattagctt tctcaatcgc
    12721 gattcatgtt gatccagaaa ttctcattgt tgacgaagcg ctcgcagtcg gtgacgcacg
    12781 ttttgtagca aaatgtatgc gccgtattaa agatattatg agtaaaggaa caaccgtact
    12841 ttttgtgagc cacgacatat cttcagttcg gacgctatgc agccgcaccc tgtggcttga
    12901 tcaaggatat ctcgtagaag atggcgatgt ttttccagta acagggcgct atatggccta
    12961 tatgtttaat aatgatcaac cttccatcga agcatccaat ttagcgcata aaaatatcgc
    13021 tcgccataaa actaaatcta caaaactatc agaactgcct atcgaaaatc aggctgattc
    13081 ttccttgcaa ggtgaaataa ccgggaatgg acagacatca ccgccagatt cacgacccga
    13141 gattgcgctc gattcaaaac ctatcaccca ctggggatct tataaaggta ttatcctttc
    13201 tgcatcggta cgggatagtc atggcgtcag aaaaaatacg ctccactggg gtgcgcctat
    13261 tgaaattttt atcgatctgc gtattccacc cgaaatttct cgggatcact taagcattgc
    13321 attttcgatt aaagatttaa aagggaccga tttaatcgtt tcgacgactc atgattttga
    13381 attaaaacgc ttacctgaag atgaacgatt cagtatttca tttaaattaa cgaatccctt
    13441 agtgaccgga aaatacctac tggtagcagc agtagaaaat cggcagtacc gtgatattca
    13501 ttattatgaa tatttcgaag gtgctcatta ctttgcatcg ctgtcgaaag agcgattttt
    13561 tggacttttc cagcctagta ttgaacaagc agttagcctg aaataatatg cttatggatt
    13621 ctatcaactc caacgcatac tggaataatc gattcaaaag taattgggat agctttgagg
    13681 ggccagcaca atctcgcttt ttttctcaaa tcgctataga acatttgcct aaatggttta
    13741 tcgagcaaat caagagaaac aatttaacct tcgccgactg gggatgcgct caaggcgacg
    13801 gttcagatgt ttgggcaagt tacattgact cctcccaaat aactggaatc gatttctcat
    13861 tagctgctat tgagaaagca acacggcgct accctgcaat ctgtttcctc aatgaagatt
    13921 ggctaagcaa aagcttaata agcaatgaaa tagtctacga tatcattttc tcgtcgaata
    13981 ccttagaaca tttccacaaa ccctatgagg tactcacctc gatattaaag cgggcaagaa
    14041 aagcggtcgt acttgcgctc ccctataaag aaatagatcg gattgatgaa catttctttt
    14101 cattcctgcc tgaaaatatc ccgttgatgc tcaagaataa ttttaggctt gttgtatctc
    14161 gtgttattga ttgcaaaacc aggccaagaa ctgcttggca gggataccag atcatcttac
    14221 tctatgccca ttcagattgg attgatcaat tattgttatc gctaaatgac tctcgtatcg
    14281 aatcttttga cgcagattca cacattgaaa atctcaataa tcaaattaaa cgcctaaacg
    14341 atgaaatcac gcaccaaact gaacaaattg tccagctcaa gcaaacaatc cttgaacaaa
    14401 atcagcatat tacccattat cagcaaacct tatcagaata taatctcaag caacaacagt
    14461 tttatcaggt tatcgctgaa cgcgatacac acatttcaaa cttagagcag acgctaactc
    14521 aacgtgatga tcaaattaat caattggaaa taaaacggtt actggaatat gaaaaaacga
    14581 tacagttatc ggattgggca caaaaaatag ataaccgccc atttcactat ggaactaaaa
    14641 aattagtgct caaatcggca aaaaacttct atcacgcact tccgatttcc catcgttata
    14701 aacaaaaatt taagtttttc ctgtctcaaa taccctattt aaaaaaaata ataaaaacat
    14761 ttccagtatc tgtaaatcaa atatctcgaa gtgcattaga ccataaacca ctcgttccag
    14821 ataatttacg tgccttaatc aatggagaaa aattaaacag tcgatctgat tacttaattt
    14881 tctcagtaat tgattggcac ttccggctgc aacgacctca acatcttgcg cgaagtttag
    14941 ctaaactcgg gaaacgtgtg ttctacttca gcaatcattt cattgattca ccaacacctg
    15001 ggtatgcgat cgaacgattg gatagcgact tggagcttta ccagatcaaa ctctatgtag
    15061 aggatgcgcc agcaatctac ttttcctcac ctacggttaa gcatctaaat aatttgcagc
    15121 tgagcatcac gaattttttt attgattttg caacgctatc aagtgtttca attattcagc
    15181 atgcatactg gtatccttta gcaataggcc tacccaactc acttcgaatt tacgattgta
    15241 tggatcacca tgaaggattt ggaaatgtat cagaagatct aattaagctc gaaaaagaga
    15301 tgatgtgtaa atttgatcaa atcatcgttt cttccgcatg gctagaaaag caggcccagc
    15361 aatataataa taatgttgta atcatccgca atgctagtga gtacgagcat tttaataatc
    15421 cacccattga aatctacttg gatcccaaag gtcgtaaaat aataggctat tatggtgcga
    15481 ttgctgagtg gttcgatctc gaattagtaa aaaacctggc cttggccgag cctaatgcgc
    15541 taatacttct tattggtaac gataccatta atgcatctca tatttcgaag gcgataccca
    15601 atatcatttt tataggtgag gtatcctata atcgactccc attctactta tatgcttttg
    15661 atgtttgctt actgccattt aagattagcc ccctaacact tgcgacaaat cctgtgaaga
    15721 tttatgaata tctggccagt ggaaaacctg ttgtaagcgt cgatcttcca gagatttccc
    15781 aatttgaaaa tctcgtgcac cgagctaaaa cctcagatga gtttatatca ttggtttcca
    15841 cttgtttgaa atcatctagc cctgaagaaa atcaaacaga attacgcaag cagtttgcat
    15901 gtaagcaaac ttggaatcat cgtgtaaacg acttagtgca agctattcaa caaataccca
    15961 tgccaaaggt aagtgttatt gttttgacct acaataatct taatctaaca aaactctgtc
    16021 tgaatagctt actgcaatgg agcgattatc ctaatctcga aattatcatt gttgataata
    16081 attcttctga taacacacct gattatctaa ctgagcttaa tacgcgccat cccacaatcc
    16141 aagttattcg aaataaaaca aatgaaggct ttgccaaagg caataatatt ggccttattg
    16201 ccgcaacagg tgattatctt gttatgctca ataacgatac cgtggtaacg cctggatgga
    16261 tattaacgat gctacgccat ttacaggctg atccttcgat tggaataatt ggagctgtaa
    16321 caaataatat tgggaacgaa gcaaaaattg acattactta cccgctctta agtgaagaga
    16381 tgctccctcg catcttatcg catactattc ctcatatggg gaaaaatttt aagctgagaa
    16441 ctgctgcctt cttttgtgta atgatgtcta gaaatgtttt tcaacaagta ggcttacttg
    16501 acgagaatta tggtcgtggc ttttttgaag atgatgatta ttgcagacga attgatcgac
    16561 tagacctaca aattgcttgc gccgaagatg ttttcattca tcatcatctt tcagcatcat
    16621 ttaatcagat tcctgatcga gacaagcaag ccttgtttga gaaaaacaaa gcctattatg
    16681 agtctaaatg gggcaaatgg aatccacatg agtataggca ttaatagaac catactttgt
    16741 gtaattggca cccgtcccga agcaatcaaa atggcgcctg tcattcttga gttaaggaaa
    16801 aaatcatttc aagttcgcgt tctcgctaca gcccaacatc gaaacttact cgatcaagta
    16861 ctcgacttat ttggcattgt gcctaacatc gatttgaata taatgcggcc caatcagtca
    16921 cttgcaccat tgactgcacg gcttttgctc agtgcagaag aagtattagc atcagaaaaa
    16981 ccagatgcag ttttagttca gggagatact actaccgtaa tggctgttgc cttagcttgc
    17041 ttttatcagc atatcccgat tggccacgtt gaagcaggat tgagaacctg ggacatggat
    17101 aatcctttcc ctgaggaagc aaaccgtgtt attactggga aattagcgcg ctggcacttt
    17161 gcgccgactg aagaatctcg cctaaatcta atgcgtgatg gtatctcgga taataatatt
    17221 tttattaccg gaaataccgt tattgacgcc ttattatcgc ttgctgatca gaatctcgca
    17281 ttacctatta accttgatcc ggataaacga ctcgttctca ttactgccca tcgtcgtgaa
    17341 aattttggtg agccactccg atctatctgc caagctatta aatctttggc ccagaacaac
    17401 cctggtgttg aatttctcta tccagtgcac cctaacccta acgtaactga aaccgtaaat
    17461 gaattgctcg cgagccagaa aaatattcat ttatgtgctc cccttgatta cgcacatttt
    17521 gttgcagtga tgaaacgttc ttttttgatc ctaaccgact caggaggaat acaagaagaa
    17581 gcacctgcac tcagcaaacc cgttctcgtt ctacgcaatg aaacggagcg ccctgaagca
    17641 gtaactcaag gtgttgcaaa acttgtaggt catgattacg atagaatact aagcgagaca
    17701 cagcgtctgt tggatgatga attagcttat ttcgccatga gaaaaggaat ctcaccctat
    17761 ggagacggcc atgcagcgga gcgcattgta aaagtgctag aggagcggct tccttaatgc
    17821 aactggttta tctttcaccc ttaccatggg atagctttag ccaaagaccg catcagtttg
    17881 ttgaatggtt tcacaacaag catggcggtg aggtactttg gatagaccct taccccacaa
    17941 ggttacccaa tctaggggat ttccgaagaa taaaatcatc aaaattttta tcaaaaaatg
    18001 taatcgtggg caatggtaat aagccaccgt ggttaacggt aattcaacca tttgttccgc
    18061 ctatagagcc actaccgggc tcttgttatg tgtatcgctg gctatggaat gatattctcc
    18121 aagcaatcga tcgtttcatt gaatctcaga acgaaacgtt attaggtatt ggcaagccct
    18181 cagaactggc gttacaagta ctattccgtt acccttccct caagtcgata tacgatgcca
    18241 tggatgattt tcctgcgttt tatcacggca tatcgagatt tgcaatgatg aaccgtgagc
    18301 aacggatagc aaaacaagta acacgcatat cagcttcttc gactaagctc actgaacgtt
    18361 ttattgaata tcaatccaag ctaacccttg ttcataacgc ttgtgccgtt gatgcccttc
    18421 cctctatcaa cgatacgcta aaaactcgtg cgacagaaaa acaagtactg gggtacctag
    18481 gaacgattgg ggattggttt gattgggcgt ttgtagctaa actcgccgaa acggtcagta
    18541 cctcaacaat ccatctaatt ggtcccatct ttaccaaaat cccagcctcg ctccctcaaa
    18601 atatcaaatt tttacctgct tgcgaacaca gcgctgcaat tcaagcgatg ttatcgttta
    18661 cagttgggct cattccattc aagcgtaatg acttaacagc agcaatcgat cccattaagt
    18721 attatgaata ccgctcctta ggaattccga ttctttcaac tcatttcggt gaaatgtcct
    18781 tgcggcaagg tcaaccaggg atttttatcg caaacgccag ctgtgatctc ggcgatcaca
    18841 tcaataaagc gatggactac aaatacccta agtcagaaat taattggttt agaaaaataa
    18901 attcatggga agcgcgattt aactcgggca atatactccc ccaaacctaa tcaagatgta
    18961 ccatctatcc ccatttaggg cttataggca agaaaccaca agctaaacgc aattagcata
    19021 cccgttgatg caaggtaagc atactgcgat gcacccgtca gcttagcatg agcaggcacc
    19081 tcagatcgtt tcaaggccat gtaattcaag atagccaaaa cgggcgccaa tacaaatgaa
    19141 acggttgtcg ctaaatcaac catcgctcgc atatctcgta gaaaaaaatg gagtaataca
    19201 atcgtaccca gtgcggttaa tatcaaccaa taccagtata ttttattgtg atagggcaaa
    19261 ctatcgagtc ggggaaacag caattcagtc gatcgacgta atgtgcgtgg ataagcatcc
    19321 agacaagtca acaacgtact aaacatggtc gcaaatgctg ctaaagcaat gatcggaaac
    19381 gcccattccc cgaggctagt cgtataaaaa ccaataaact gtttagcaaa ctctgtgcta
    19441 ttactagccg gtgattccgg agttccatac atgacgagtg cgccgagagt aagaaaacac
    19501 actgccagta ccattgttcc ccaataacca accttgaagt caagcaaggc ttgagccaat
    19561 ggaatgcgat gaccctgctc cttgttttta gaaacagacc acaccgaatg ccaaatcgaa
    19621 atatcaatgg gtgcaggcat ccacccaatt aatgcaacga gaaaaagtat atgcgctgat
    19681 tccaagaaat taaatgtcgt ttcaaactca gcttgctttt caatgggcgc gaagaaagaa
    19741 aaaataagtg caagtaatgt agagatcgtt aggacgatca tgatgaactt catgctgcga
    19801 tccaggagcg aataatgacc aaaccctagt atttgtaccg acaaaactaa taacaacata
    19861 gcaataattg gggcatccag aggcattcct gttagctggg caaataatcc tgctgtcaca
    19921 atagaaatcg cccccatcac aataaacatc gtcaacgcac tgagtaagaa ataaatccac
    19981 acggcccatg caccaatctg gtgatagcca tcaagcaatg ttttacctgt tgcagcggcg
    20041 tagcgaggac ctaactcaaa gattgggtat ttaaggatat tcactaaaat cacaatggct
    20101 actaactgga agccaaactc agcccccgct cgcgtactct gcaccaaatg cgaaacacca
    20161 acagcagccc ccgcatagag caatcccggg ccaaactgac taagtttttc tttcagcgac
    20221 attgataact tcctttttta gtattgtgaa ttcagattgg aaatgcataa aagcgcaact
    20281 tttgatagtg gcttggtata taacctaccg acaccctgaa gaatatctta taagaaattc
    20341 tgaatcaaag tactttgcct gatcaggaat tgaagtacct agctacgcaa tgggcctatt
    20401 ctttaataaa attgtcaccc aggctagcaa aaaagtttgc agcaatcgtt ttatcatagt
    20461 tattaccaag ctgtagcggt aaggacaaac tcccatttcc tgatatcgct tgctaagctc
    20521 aatagtgtct tcaatctatt gtgaaatgac gctacgccta tgaagcatgt cttttagaga
    20581 ggagggaact ccgctcacaa cctccttttg tttgt
//
LOCUS       DAOQLJ010000023        19675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_963_length_19675_cov_6.647452, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000023 DAOQLJ010000000
VERSION     DAOQLJ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 19675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19675
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_963_length_19675_cov_6.647452"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..723
                     /locus_tag="PK522_03050"
     CDS             46..723
                     /locus_tag="PK522_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40745.1"
                     /translation="MKQLVLLIIFLFSITGCATRYGDHRADYARQNPASVKTTDIALE
                     ATHDVVVPESSQGLNSAPLACLAVFPITAKESDLEGAESIRSALHAQLAPTGIRLVAL
                     QRIDAAIKMTDNSLGELNANVAKSIGCDTIILGEVTERVSSQYGIYSEARAGVDIKII
                     RASTGDVIWKGSHLAVIRGGGIPLGMVNVPLNVVFAGLNLYGDRDAWVVHDLARRVVS
                     AIPGLIY"
     gene            947..1591
                     /locus_tag="PK522_03055"
     CDS             947..1591
                     /locus_tag="PK522_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40746.1"
                     /translation="MKLLAPLILCVLSITGCATRNAGSSSDSVKQNDVDVVESSQELK
                     SNPLSCIAVFPLAAKENDIKGAELIRKELHAHLAPTGIRLIALQRVDAAIKMTNNSLG
                     DLNANVAKSIDCDTIMVGEVTEKSSKYYGVYSEVRAGANIKIIRASTEEILWQGSHTA
                     VIRGGGIPFGIGSFVMNSVFAGMNLYGDQSVWVVQDLARRIVLAIPNLAYRGED"
     gene            complement(1698..1799)
                     /locus_tag="PK522_03060"
                     /pseudo
     CDS             complement(1698..1799)
                     /locus_tag="PK522_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008182253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1886..2248
                     /locus_tag="PK522_03065"
     CDS             1886..2248
                     /locus_tag="PK522_03065"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNC40747.1"
                     /translation="MSNGDDTIISCFVVGIAGCSDNEMGFLFREEGITAASPGVFTNV
                     QPNDYWSVTEFAIPFSAWNFNFNNGNRFVDGMNFNFFAWAVHSGDVGAQVPEPGTLAL
                     MGLGLMGLLGFGRQQRRR"
     gene            complement(2616..3611)
                     /locus_tag="PK522_03070"
     CDS             complement(2616..3611)
                     /locus_tag="PK522_03070"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNC40748.1"
                     /translation="MDTHVLANPKLAAILGGLVADAASLGLHWLYDPNRIAEIEKESD
                     IAFRQPNENDYANVSGFFAHGSKVAGDSSGYGEIGLLMLKHLAKHGQFNHMAYQLEFR
                     AFFGPGGEYTGYVDSPTRQTIRTLLPLDAKSFPATSGADDDQMPTLATVPVLVATHQG
                     TVDSLMKQVEEVVRITNHNDIAVSAAQCAAALLNGILMGKPLNQAATDALPLAEDNLR
                     LLLEQALSLEALDSVAAANRFGSACHVTEGLPIVFHIAKHAHDYRTAINANIRAGGDS
                     CGRSIILGALVAAHLAAQNRANTGIPLPWLARYRKLTMAADACQAIMDVPTPKAR"
     gene            complement(3658..4494)
                     /locus_tag="PK522_03075"
     CDS             complement(3658..4494)
                     /locus_tag="PK522_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNC40749.1"
                     /translation="MKLKGLGRGLDALLTGSSINQALSESLQNLDIAQLQPGKYQPRT
                     NMDQSSLMELSKSIKAQGIMQPILVRAIDTERYEIIAGERRWRAAQLAGLTQVPVIIR
                     EVADESALAMSLIENIQRENLNPLETAMGIERLIREFGMTHQTAGDALGSSRSAITNL
                     LRLLNLASPVQDLMMQGKIDMGHGRALLTLNATKQIEIAHLIANKQLSVRETEKLIKR
                     IEQVPLKKPIIQNRDLLRLQEDISTKLGANVTIKPGKHGTGNLVIHYTSLEQLDGILA
                     KF"
     gene            complement(4538..5302)
                     /locus_tag="PK522_03080"
     CDS             complement(4538..5302)
                     /locus_tag="PK522_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNC40750.1"
                     /translation="MVKILAIANQKGGVGKTTTSINLAASLVNAGKRILLIDLDPQGN
                     TTMGSGIDKRFLQHTIYHVLLGEQSIANVRISETKGRYHLIPANQDLAGAEIEMVGLD
                     QRETRLKNALQEVIADYDLVLMDCPPALNLLTLNGLCAAHAVMIPMQCEYFALEGLSD
                     LVNTIKRVRASFNPALKIEGLLRTMFDPRNILAKQVSDQLQQHFGKKVYQTVIPRNIR
                     LAEAPGFGLPVLLHDKHSKGAQAYLDLAKEVLNAAA"
     gene            complement(5322..5969)
                     /gene="rsmG"
                     /locus_tag="PK522_03085"
     CDS             complement(5322..5969)
                     /gene="rsmG"
                     /locus_tag="PK522_03085"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNC40751.1"
                     /translation="MNLEKQLLEGLEAINCGTSDRKALCTRMLHYTALIEKWNQVHNI
                     TAVRKPEEMVSKHLLDSLTLLPHIIGQYVADVGSGAGLPGIPLAIANPDLRVTVIESN
                     QKKIAFLDQARLELALSNVKVTPVRVEKYFPTQRYDTVVSRAFSDLATFVKSAQHLCV
                     EDGKSRLIAMKGRFPDAELMQLPENCIVERVIRVNVPGLRAARHLIIIKPEQRTH"
     gene            complement(5944..7863)
                     /gene="mnmG"
                     /locus_tag="PK522_03090"
     CDS             complement(5944..7863)
                     /gene="mnmG"
                     /locus_tag="PK522_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNC40752.1"
                     /translation="MVFSEKFDIIVVGGGHAGTEAALAAARTGRKTLLLTHNLDTLGQ
                     MSCNPSIGGIGKGHLVKEIDALGGVMAEAADEAGIQFRTLNSSKGLAVRATRAQADRV
                     LYRQAIRSRLENQPNLWLIQQSVDDFILEGDKIAGVVTQLGIRYSARAVILTAGTFLA
                     GLAHIGSAHFQAGRAGDPPSISLAHRLRELNLPVGRLKTGTPPRLDGRSINYALLQEQ
                     PGDYPTPVFSFIGNVSQHPRQISCWISRTNDQTHEIIRNGLDRSPLFTGKIEGVGPRY
                     CPSIEDKVVRFSERDSHNIFLEPEGLNTHEIYPNGISTSLPYDVQIELVRSIAGLEHA
                     HITRPGYAIEYDYFDPRALKSSLETKAIEGLFFAGQINGTTGYEEAAAQGLLAGLNAS
                     LKLQGHEPWCPLRNEAYLGVLVDDLITRGVTEPYRMFTSRAEYRLQLREDNADMRLTE
                     IGYRLGLVSEARWRTFSHKQATIELEQQRLRSYWVRPGMAPEEHQVKILGNSIEKEYT
                     LYDLLRRPNVTYDGLMTLFQIEDNVSNPSVAEQVEISAKYEGYIERQRQEVARHQHYE
                     TTRLPIGIDYHQVSGLSNEVRQKLNLHKPETIGQAARISGVTPAAISLLLVHLKRGFI
                     LSNSNQDYESGKTTA"
     gene            8206..8415
                     /locus_tag="PK522_03095"
     CDS             8206..8415
                     /locus_tag="PK522_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HNC40753.1"
                     /translation="MGKIVIDIYESEHTKFMRELFKQRPHLVEQQKEARAIWWDKKVD
                     LETQKHFKEAKVPQTSYVYFDWLQK"
     gene            8513..9802
                     /locus_tag="PK522_03100"
     CDS             8513..9802
                     /locus_tag="PK522_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HNC40754.1"
                     /translation="MEALSLPLLLLLLVVLLILSGFFSLSETSMMAINRYRLKHLAKQ
                     GHRGAQLASKLLENTDRLLGVILLGNNLLNTAAATLVAVIVSMLFAHSDLALLIGTLA
                     VTFAILVFSEITPKVIAATYPEKIALAASYILTPLLKIFYPAVWFVNLFVQGILSLLR
                     LHPKEHTAHKISPEELKMLVLEAGHFIQTKHQNILVNLVDLETVTVDDVIVPRKQIEA
                     IDLDAEDEVIHEQIMTCHHTRLPVYRERLDNVIGFIHVRKIFNQLKGERITANIIERV
                     LQEPYFIPSGTRLFSQLQLFQENRKRIGFVVDEYGEWLGLVTLEDIIEEIIGEFTTHA
                     PTQTSAFIQEPDGSIIAEGSVLLRDLNRKLGYQLPLDGPKTLNGLILEHFQDIPEAGI
                     GLNIAGYPMEIIQTKDQAVKIVRIHPTIPKIVGDKIE"
     gene            9823..9899
                     /locus_tag="PK522_03105"
     tRNA            9823..9899
                     /locus_tag="PK522_03105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9857..9859,aa:Arg,seq:ccg)
     gene            complement(9930..10379)
                     /locus_tag="PK522_03110"
     CDS             complement(9930..10379)
                     /locus_tag="PK522_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC40755.1"
                     /translation="MIASILFLSSCTESVTTATKLLQQLQEQGLMNEVKRDFDAKQVT
                     RGHEIFIKNCAVCHGQNGEGTADWRKPLANGRYPPPPLDSTAHAWHHSTEELKRFILK
                     GGPPGEGRMPEWQDKLSEQEIDDILVWIKSLWSDEIYEAWYRQIEHR"
     gene            complement(10425..10889)
                     /locus_tag="PK522_03115"
     CDS             complement(10425..10889)
                     /locus_tag="PK522_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HNC40756.1"
                     /translation="MKTNKRQFILTSILTIHLVITTNFVSAAGTVEVYKSPTCGCCGK
                     WVDHLRDHGFTVNTHDVGNKEARARAGISPTLGSCHTALVDGYAIEGHVPAQDIIRLL
                     KERPKAVGISVPDMPHGTPGMEGERNDPYDVLLIQEKGDTRDKASVFNRYRP"
     gene            complement(10886..11344)
                     /locus_tag="PK522_03120"
     CDS             complement(10886..11344)
                     /locus_tag="PK522_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HNC40757.1"
                     /translation="MKKSLFMLYFILISWTLNTFADTGTHPEHSLIGQSGDAAQVSQT
                     IEIKMIENRFIPDEITIKQGETIRFLVKNDGKKHHEFAIDTIENLKEHAKMMRAHPNM
                     AHNDPNRIKIAPGEQKELIWQFTETGIVDFACPFPGHFKGMRGKIYVEKK"
     gene            11530..12468
                     /gene="ttcA"
                     /locus_tag="PK522_03125"
     CDS             11530..12468
                     /gene="ttcA"
                     /locus_tag="PK522_03125"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HNC40758.1"
                     /translation="MQEILSKKTQYNNNKLHKRLRRLVGTAIADFNMIEPGDRIMVCL
                     SGGKDSYGLLDILLNLQAHAPLDFELVAVNLDQKQPGFPEHVLPEYLARISVPFRIVE
                     QDTYSVVKRVISEGKTTCSLCSRLRRGVLYRVAKELGATKIALGHHRDDILETFFLNL
                     FYGGKLKAMPPKLVSDDGNHIVIRPLAYCKEKDLAAYAQVANFPIIPCNLCGSQPNLQ
                     RQVIKEMMQQWDKKFPGRIETMFTALQNIQPSHLADIARYDFQGLKAHGKADADGDKA
                     FDEEQFEPTIAALMPFSDDSSVYQVASTQDNHNGFK"
     gene            complement(12470..12856)
                     /locus_tag="PK522_03130"
     CDS             complement(12470..12856)
                     /locus_tag="PK522_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HNC40759.1"
                     /translation="MHTADIVLLIHFVFVLFVVASLPVIWLGTWFNWLFIRNFTFRLT
                     HLAAILFVVGESLIGITCPLTAWEHELRGTGSEQSFMQQWLHRILFYDFPEYVLTSIY
                     LLFALLVLITYKLAPPIAYKRNHNRH"
     gene            complement(12871..13410)
                     /locus_tag="PK522_03135"
     CDS             complement(12871..13410)
                     /locus_tag="PK522_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNC40760.1"
                     /translation="MSKFGKIVLYAVIAFSAMTAGFFFKAQQLTPQLSSDEKKRGVEK
                     FFSSGLPDVKGDMQPFSQWQGNVLLVNFWATWCAPCQEEIPEFIQAQETYRDKGLVLI
                     GIAVDQPDRVASFSRDFGINYPVVVGELDAFSLAEAMGNPQGALPFTVILNRAGEIVY
                     SHLGRIKIEEIEKIIKPLL"
     gene            complement(13451..15385)
                     /gene="dsbD"
                     /locus_tag="PK522_03140"
     CDS             complement(13451..15385)
                     /gene="dsbD"
                     /locus_tag="PK522_03140"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HNC40761.1"
                     /translation="MRLFPFLFVLFFFTVNPAIAQEEGRSGVMTLLQKLGANFGQAEQ
                     ELLPPDEAFKLAIEVKDEHTLIAHFTPAKNYYLYRDKIAFEPQSEGIQIEKISLPPGK
                     MKDDLTFGKTEVYYQPFQAVIALKRGIEAPDQLSLASTYQGCNEPVGVCYAPIKKINE
                     VTLPAIKAAVNTVAETLSGNAAAAPIDSTAALFQTPSRPPAIETESFKIEQMFASGSF
                     GLILAGFFGIGLLLSFTPCVFPMFPILSGIIANRSQHMTKGRGFVLALAYVLGMAIAY
                     AIAGVAAGLSGAMLSAVLQNAWVLGTFALIFILLAFSMFGFYELQLPSFLQSKISEEA
                     GHLHGGHLSSVFGMGALSALIVSPCVAAPLAGALLYISQTRDIVLGGSALFVMALGMG
                     VPLLLIGASAGALLPKSGPWMESIKQFFGVLLLAVAIWLISPVVSTVIYMLLWAALLI
                     ISAIYLHAIDPLPVNALGFRKFLKGVGVIALLTGVALLIGVLSGSRDVLQPLSKLSIT
                     SNSSNVSFSEKGWDQLAHLPFQPVRSVTELDEIIAQSHDKYIMVDFYADWCISCKEME
                     RFTFSDERVQAKLKDVVLLQVDVTHGTPDDAALLKRFKLFGPPGILFLDRNGTEIPNI
                     KIIGYLNKDDFLAVLNAVLI"
     gene            complement(15418..15735)
                     /gene="cutA"
                     /locus_tag="PK522_03145"
     CDS             complement(15418..15735)
                     /gene="cutA"
                     /locus_tag="PK522_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNC40762.1"
                     /translation="MEAILILTSFPEEKGALRLAKQLIDQHKAACVHIGSQLTSIYHW
                     KGKTETATEVPVVIKTLSAHYAAVEQTIKALHPYELPEIIAVPIIKGLPAYLQWIADE
                     TVH"
     gene            15872..16081
                     /locus_tag="PK522_03150"
     CDS             15872..16081
                     /locus_tag="PK522_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40763.1"
                     /translation="MIKRLFLLLQFLAFIAPIGIFLMYIIMDEGDQFTYEHYWVTAMS
                     FIPFVFVLLIKYMFSDLDQNKKDDR"
     gene            16078..16479
                     /locus_tag="PK522_03155"
     CDS             16078..16479
                     /locus_tag="PK522_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40764.1"
                     /translation="MKKPILLIGLLILGIVEWVQISASSNTEHEIFSAEAVEKITIDA
                     GFGWGIFGGSVYNGNSDYTITQITVKLTPSQIIGTSSTSPLVAERYHIDITVPPLTKA
                     ALSMPLDADGTLEFLWELVQVQGYRVSVKSN"
     gene            16542..16784
                     /locus_tag="PK522_03160"
     CDS             16542..16784
                     /locus_tag="PK522_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40765.1"
                     /translation="MIVRQNIVLIIVALFTFSNSAVATNQSAEEKNQTSLITDSAQQA
                     VQPSKHSILKEKKSEQPESTKPIKPNMADYCRKHTC"
     gene            16806..17582
                     /locus_tag="PK522_03165"
     CDS             16806..17582
                     /locus_tag="PK522_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNC40766.1"
                     /translation="MKLMTNLSNYILAASLTLSIANAAEWSYEEQKRWGSIEDHNQHE
                     VPHIYPYAVCSIGQHQSPVDLATSQVSKRLNNLKFRYYADKPDFFNTGHAVQVNLSND
                     YQGELLVGKEAYPLIQLHFHEPSEHVIGTKIFPAELHFVHVRDDGKIAVLGVLLEIGK
                     DNPVFQTILENTPPQGGELKQDSDVWLSITSLLPEKRDEYYTLAGSLTTPPCSEGVDW
                     YVLTDTTEISEAQLDRLKSFFSGNARLKQELNNRAIAKKK"
     gene            complement(17608..>19675)
                     /locus_tag="PK522_03170"
     CDS             complement(17608..>19675)
                     /locus_tag="PK522_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC40767.1"
                     /translation="WALSGANDGYWDWNMETNYVYYSSRWKAMLGYSDRELLSPSLKT
                     WEKLVNPEDKSRVLKLVDDYVTGRKDKFETEFKMKHKGGHWVTVLSRASLARDNSGNL
                     IYPKRLIGTHVDLTERKQSELQLKIQNIALEAAANAIMITDTAATIEWINPAFTELTG
                     YRRHEAVGMNVKDLMNSGQQSELFYKDMWQTISSKKVWKGEIINKKKDGALYHEHMTI
                     TPVTNESDEICYYIAIKEDISQRKASEKEILDLAFYDALTKLPNRRLFLDRLRHTVTA
                     CQRNKTYNALLFIDLDNFKFLNDTHGHDAGDALLIEAAKRITSCIRETDTVARLGGDE
                     FVVMLEQLGSDDSHAMNITTQIAESILSRLHQEYRVNNIRHRGSSSIGICMFSDQYSK
                     ADDILKWADKAMYEAKCAGRNTLRFFDPHMQQELERRTQLEKELWIAVDKHQFRLHYQ
                     LLINQSQQIVGAEALLRWQHPDKGIVGPNEFLSLIENNGYIIPIGDWIIDEGIRQLQF
                     WQQHDTLKSLTLSLNISAVQFQESGFVQKLKRKIEQSQIDHSRLILELTESSLINHID
                     DTVKKINELRTLGIRLSIDDFGTGYSSLSYLKRFKLAQLKIERMFVRDILTDQDDLTL
                     VKTIINMGKNLGLDVVAEGVENQQQFEILKNAGCAKFQGYWINKPIPGEQITALVTSK
                     HQQHVH"
BASE COUNT         5480 a         4108 c         4263 g         5824 t
ORIGIN      
        1 atcctcccgc tatttaaccc cgattatctc taaacttaac gtaaagtgaa gcagttagtc
       61 ctcttaatca tttttttatt tagcattaca ggctgtgcga ctcgttatgg tgatcacagg
      121 gctgattatg ccagacagaa tccagcgtcg gtcaagacca ctgatatcgc ccttgaggct
      181 actcacgatg ttgttgttcc cgaatcttct caaggattaa attctgcacc tctggcttgc
      241 cttgcggtat tccctattac cgccaaggag agtgatcttg agggagcaga gtcaattcgc
      301 agtgcgctac acgctcagct ggcacccacg ggtattcgtt tggtggcgct ccagcggatc
      361 gatgctgcga ttaaaatgac tgacaactca ttgggtgagc tgaacgctaa tgtggctaaa
      421 tcaattggtt gtgacacgat catcttgggt gaagtgactg agagagtgtc aagtcagtat
      481 ggtatctact ctgaggctcg tgctggggtc gatataaaaa ttattagagc ttctacaggg
      541 gatgttatct ggaaaggtag ccacttggca gtaattagag gtgggggaat ccctttaggt
      601 atggttaatg ttcctctaaa tgtggttttt gcaggactga atctttacgg tgaccgggat
      661 gcttgggttg tccacgatct agctaggcga gttgtgtcag caattccggg tttgatttat
      721 taagctgaag ccttgcccag taaaacttga ttggtcttga tccattccag tctcgattct
      781 caataaaatt tagacaaaat taatacgctg atatcccccg cctttcttat ttacttacga
      841 actcgttgtc tttaagatga aagtacgaaa gatacttctc aatgagaggt tctccgttaa
      901 aaaaagtgat caatccacgt ggatttaaca gaagaaactc gattcaatga aactgctagc
      961 gcccctaatc ctctgcgtac tcagcattac aggttgtgca acccgtaatg ctggaagtag
     1021 tagtgattct gtcaaacaga acgatgtcga tgtcgttgaa tcgtcccaag aattgaagtc
     1081 taatcctcta tcgtgtatcg cagtatttcc tctcgcggcg aaggagaatg acattaaagg
     1141 ggctgaattg attcgtaaag agctacacgc acatttggca cccactggca ttcgtctgat
     1201 agcccttcaa cgggtcgatg ctgcgatcaa aatgactaat aattcgttgg gtgacttgaa
     1261 cgctaatgta gccaagtcaa tcgactgtga cacgattatg gtgggcgagg taactgaaaa
     1321 atcatcgaag tactatggtg tttactctga ggtccgtgcc ggagccaata taaaaattat
     1381 cagagcctcc acagaagaaa ttctttggca aggtagccac acggcagtga ttagaggtgg
     1441 tggtattccc tttggtatag ggagctttgt tatgaattct gtttttgcag gaatgaatct
     1501 ttacggtgat cagagcgttt gggtagtcca agaccttgct aggagaattg tgctggcaat
     1561 tcccaatcta gcttaccggg gggaagacta gcggcttgct cactgttgac atccatttaa
     1621 ggctgactta ggtttccctg tactgaccgg agtgcatatg gctctcgggc aatacggaga
     1681 gtactgacgt cgaaaatttc gattaaacgt tcatacggag tttttcctcc caaacctcca
     1741 tgaggtcggt gatagttgta gaaagcttcc cactcggcta atttggcatg cagattgaca
     1801 gggcaaggta cttggaacga tcaacaagcc tgggtttcag gtcttactat tggcggaata
     1861 ggcggttggc ggctacctag cgttgatgtc taatggtgac gatactataa tcagttgttt
     1921 tgttgttggg attgctggtt gttctgataa tgaaatgggg tttctgttcc gtgaagaagg
     1981 cataacagca gcatcaccgg gagtattcac caacgtccag cccaacgact actggtccgt
     2041 tacggagttt gcgattccat tcagcgcgtg gaacttcaac ttcaataacg gcaaccgttt
     2101 cgtggacggc atgaacttca attttttcgc ttgggctgtt cactcgggtg atgtcggcgc
     2161 gcaggtacct gagccaggta cgctggcact gatgggactc gggttaatgg gactacttgg
     2221 cttcggacgg caacagcgtc gacgttgata ctttgatgat tcgggcgctt cggctctttg
     2281 aataggcaca ggtggaagcc tcccttgctg tgccgatcct atcgggaatt atcggaatat
     2341 tttacagtga tgtaagtatt tgaagggtgt ggagtcgaac ctccgaccta ccaattacga
     2401 agagaacccc accttaaaac tgtggggttt tttattattc atactctaag ttaatctcta
     2461 ccctttccag atcatcaaga ttatcttctt taacgtgaag aataagattg attccagcca
     2521 gtcgcagacc ctctcagccg ttgtataaac gcctattctt ttaaggaaca cacaaaactt
     2581 agctgtaatt ctgtcagtag ttggctagaa cctttctacc tagccttagg ggtaggtaca
     2641 tccatgatcg cctgacaagc atctgctgcc atggtgagct tacgataccg cgcaagccag
     2701 ggtaacggaa ttccagtatt tgcgcgattt tgtgcagcga gatgggctgc tactaatgca
     2761 cccaatataa ttgaacggcc acagctatcg cctcctgccc gaatattagc gttaatggct
     2821 gtcctataat catgggcatg ttttgcaatg tgaaaaacga ttggaagtcc ttccgttacg
     2881 tggcaagcgc tgccaaaacg gttggctgct gcaacactat ccaatgcttc tagcgagagt
     2941 gcttgttcga gtaataatcg caaattatct tcggctaagg gtagtgcatc ggttgcggct
     3001 tgattcaagg gtttccccat taaaatacca ttgagcaatg cagcggcaca ttgggctgct
     3061 gagacagcaa tatcgttgtg attagtaatg cgcacaactt cttctacttg cttcatcagt
     3121 gaatcaacgg ttccttggtg tgttgctact aaaacgggta cggttgctaa ggtcggcatt
     3181 tgatcatcat cggctccgga agtcgctgga aaacttttgg catccagtgg caacaaagtc
     3241 cgtatggttt ggcgcgtggg agaatcgaca taacccgtgt attctccgcc ggggccaaag
     3301 aaagcgcgaa attcgagctg ataagccata tgattaaatt gcccatgctt ggctaggtgc
     3361 ttgagcatga gtaaaccgat ttctccgtaa cctgatgaat ctcctgcgac tttgctacca
     3421 tgcgcaaaga agccgcttac attggcataa tcgttttcat tcggttgacg aaaggcgatg
     3481 tcagattctt tttcaatttc cgcaatccgg ttaggatcat atagccaatg aaggcctaat
     3541 gaagcagcat cagcgactaa tccacctaaa atggcggcaa gtttggggtt tgctaaaaca
     3601 tgagtatcca atgctgactc caggtgaatg aattagaaaa gcgtacaatt gttttggtta
     3661 aaacttggcg agaataccat cgagttgctc aaggctagta tagtgaatca ccagattacc
     3721 tgttccatgc ttgccaggct taattgtgac attggcacct aatttggtag aaatatcttc
     3781 ctgtaagcgt aataagtcgc gattttgaat aatgggtttt ttcaatggaa cttgctcgat
     3841 acgcttgatt aatttttcgg tttctctgac ggaaagttgt ttattcgcga taagatgggc
     3901 aatttcaatt tgtttagtag cattaagggt aagtaaggca cggccatgtc ccatatcgat
     3961 ttttccttgc atcatgagat cctgaacggg tgacgcaaga ttcaatagac gtaataagtt
     4021 ggtaattgcg cttcttgagc tacccaacgc atcccctgct gtttgatgcg tcataccaaa
     4081 ctcgcgaatg aggcgctcga tacccatggc agtttcgagt gggttgagat tctcccgctg
     4141 aatattttcg ataagcgaca tcgcaagcgc cgattcatca gcaacttcgc ggataattac
     4201 cggaacttgt gttaatccag ctaattgtgc agcgcgccaa cgcctttcgc ccgcaataat
     4261 ttcataacgc tctgtatcga tagcacgcac caaaatcggc tgcataattc cttgcgcttt
     4321 gatggattta gataattcca tcaatgaaga ctgatccata ttggtacgag gctgatattt
     4381 ccctggttga agctgagcaa tatcaaggtt ctgcaaggat tcagacaaag cttgattaat
     4441 tgaactaccg gttagtaatg catctaatcc cctacctaag ccttttagtt tcattaaatt
     4501 gagccctcag aattttattg cgtcttgata aaaacgatta ggctgccgca tttaaaactt
     4561 ctttcgccaa atctagataa gcctgtgcac ctttagaatg cttgtcgtgt aagagtacgg
     4621 gcaagccgaa cccaggagct tccgctaagc ggatattgcg tggaataacg gtttgatata
     4681 ctttttttcc aaaatgctgt tgcagctgat ctgagacttg tttagctaag atgttgcgcg
     4741 gatcaaacat ggtccgtaat aaaccctcaa ttttaagcgc cgggttaaaa ctagcgcgga
     4801 cacgtttaat cgtgttgact aaatcgctaa gcccctctag tgcaaagtac tcgcactgca
     4861 tgggaatcat aacagcatgg gcagcgcaga ggccattcag cgtcaataaa ttgagcgcag
     4921 gtgggcaatc catcagaacc agatcataat ctgctattac ctcttgcaat gcatttttta
     4981 atcgcgtttc tctttgatca aggccgacca tttcgatttc ggcaccagca agatcttgat
     5041 tcgctggaat cagatggtag cgacctttgg tttcgctaat acgaacattg gcaatcgatt
     5101 gttctccaag caatacatga taaatagtat gctgcaaaaa tcgcttgtcg ataccacttc
     5161 ccatagtggt atttccttga ggatcgagat cgattagcaa aatgcgcttg cctgcattga
     5221 ccaagctcgc cgcaagatta atgctggttg tcgtttttcc tacccctcct ttttgattgg
     5281 cgatcgcaag tatttttacc acttgataaa ctccttcatg gttaatgggt acgctgttca
     5341 ggcttgatta taatgagatg gcgtgcagcc cttagaccag gaacgttgac acgaatcaca
     5401 cgttcaacga tacaattctc aggcaattgc atcaactcgg cgtctgggaa tcgacctttc
     5461 attgcgatta accgactttt accatcttcg acacataaat gctgtgctga tttaacaaaa
     5521 gtggcgagat cagaaaaagc ccgagatact actgtatcgt atcgctgtgt tggaaaatat
     5581 ttttcaacac gaacgggagt aactttgaca ttacttaatg caagctcaag ccgcgcttga
     5641 tctaaaaaag caatcttttt ttgattgctt tcgataacag taacgcggag atccggatta
     5701 gcgattgcca gcgggatacc gggtaaccca gcaccgctcc ccacatcagc tacatattgc
     5761 ccaataatgt gcggtaatag ggttaagcta tctagcaaat gcttgctaac catttcctcc
     5821 ggtttacgca ccgctgttat attatgaacc tgattccatt tctcaatcaa agcggtatag
     5881 tgcagcatgc gtgtgcataa cgctttccga tctgatgtgc cacagttaat agcctcaagg
     5941 ccttcaagca gttgtttttc cagattcata gtcttggttg gagtttgata atataaaacc
     6001 acgcttcaag tgcacgagca ataatgaaat tgctgctggt gtaactccag aaatacgcgc
     6061 agcttgtccg attgtttcag gtttgtgtaa attaagtttt tgccgaactt cgttggataa
     6121 tccacttacc tggtgataat cgattcctat cggtaagcgc gttgtttcat aatgctgatg
     6181 gcgagcaacc tcctgtcgtt ggcgttcaat atagccttca tattttgcgc ttatttctac
     6241 ttgttcagca acgctgggat tgctcacgtt atcttcaatc tgaaatagcg tcatgagacc
     6301 atcatatgta acgttaggtc tgcgtaagag gtcgtacagg gtatactctt tctcaatact
     6361 attgccaagg attttgactt gatgttcttc aggagccatg cctgggcgaa cccaataact
     6421 tcgtaatcgt tgctgctcca gttcgatagt ggcttgtttg tgggaaaaag ttcgccaacg
     6481 cgcttcactg actaacccca atcgataacc aatttccgtc aggcgcatgt cagcattatc
     6541 ttcgcgtagt tgtaatcgat attcagctcg actggtaaac attcgatacg gctcagttac
     6601 accgcgagta attaaatcat cgactagaac accaagataa gcttcatttc tgagcggaca
     6661 ccagggctca tgtccttgga gttttaggct tgcattaagt ccggctagta atccttgcgc
     6721 ggcggcttct tcataaccgg tagtgccatt aatctggccc gcaaaaaata atccttcaat
     6781 cgcttttgtt tctaaggagc ttttgagtgc acggggatcg aagtaatcat attcaatggc
     6841 gtagccaggg cgagtaatat gcgcatgctc caatcctgca attgatcgta caagttcaat
     6901 ttgtacatca taaggtaagc tagtcgagat cccatttgga tagatttcat gtgtattcaa
     6961 tccttctggc tcgagaaaaa tattatggga atctctttcc gaaaatcgaa ctaccttatc
     7021 ttcaattgat ggacagtagc gcggcccaac cccctcaatt tttcctgtga acaaaggaga
     7081 gcgatcaagt ccgtttcgga taatttcatg ggtttgatca ttagtacggg aaatccagca
     7141 tgatatttgt ctggggtgct gactcacatt gccgataaac gaaaaaacag gggttgggta
     7201 atcacctggt tgttcttgaa gtaaagcata attgatactc cggccatcaa gtcgcggggg
     7261 tgttcccgtt tttaatcttc caacaggtaa atttaattcg cgcaaccgat gtgccaggct
     7321 gattgagggc ggatcccccg ctctgccagc ttggaaatgg gcgctgccga tatgggctaa
     7381 ccctgctaga aaggttcctg cggttagtat aactgcgcga gcagaatatc gaatgccaag
     7441 ttgggtgact acaccagcga ttttgtcgcc ttccaaaata aaatcatcaa ccgattgttg
     7501 tatgagccaa agattgggtt gattttctaa tcgactgcga atggcttggc ggtataaaac
     7561 gcgatcagcc tgcgcgcgag ttgcgcgcac tgctaaccct ttactcgaat taagggtacg
     7621 aaactgaatg cctgcttcgt ctgctgcttc tgccatgacg ccaccgagcg catcgatctc
     7681 tttaacgagg tgacctttgc caattcctcc aattgaagga ttgcatgaca tttgccctaa
     7741 cgtatcgagg ttatgggtta atagcaatgt ttttcgtcct gtgcgtgcag cggccaaggc
     7801 agcttcagtc ccggcatgcc ccccgcccac aacaataatg tcaaattttt ctgaaaaaac
     7861 cattggattg cacagatttg ggtgttttgt gaaatttaag aataagacaa gattttaaat
     7921 tatttttact cattacatat gtattttata ttgcaacgat aaaaaaggtt tcacgtgaaa
     7981 ccttttgcca gcagttttaa ttttcttgtt aaaaaagcaa gtcacaatat aatttaacta
     8041 gttgaaaaaa ataaactatc atctttaagt gcgtttagat gataagttga ttcgctgaag
     8101 atatgctatt atgagttatc ctagcgagct agacttaaga agcgtgattt gcttcattgt
     8161 cattatgata agtgttaatt gcattagaac aataaggaaa tataaatggg caagatagtc
     8221 atcgatatat atgaatcaga gcatactaaa tttatgcgag agctttttaa acaaagaccc
     8281 catcttgttg agcagcaaaa agaggcgaga gcgatttggt gggataaaaa agttgatctt
     8341 gagacacaaa agcactttaa agaagctaaa gtaccgcaaa cgagctatgt ttattttgat
     8401 tggctgcaaa agtaatctac cccatcgttt attttgctat cggtgagcaa tcaggatttt
     8461 gacttgctga ctttatagcg cctcggtcat aaattaaaag agatagcatt ctttggaagc
     8521 gctttcttta ccgttattgc ttttgctgtt ggttgtttta ttgatcctat ctggtttctt
     8581 ttcgctttct gaaaccagta tgatggcaat caatcgctat cgtttgaagc atcttgcaaa
     8641 acaagggcac cgtggcgcgc aacttgcgag taagctactt gagaataccg atcgcttatt
     8701 aggcgttatt cttttaggta ataatctatt gaatactgcg gcggctacct tggttgcagt
     8761 gattgtttca atgttgtttg cccatagcga tctggccttg ttgattggta ctctcgcagt
     8821 aacgtttgct attttagtgt tcagtgaaat tacgcctaaa gtgattgctg caacctatcc
     8881 agaaaagata gcgcttgcag cgagttacat tctgacgccg ttactcaaaa tcttttaccc
     8941 cgcagtatgg tttgtcaatt tgtttgtaca aggtatttta agcttgctac gactacatcc
     9001 caaggaacat accgcgcata aaatcagccc agaagagctc aaaatgctcg tcctggaagc
     9061 ggggcatttt attcaaacga agcatcaaaa tattcttgtc aatttggttg atcttgaaac
     9121 ggttaccgtg gatgatgtaa ttgtcccacg caaacaaatt gaagcaattg atttagatgc
     9181 agaagatgag gttattcatg agcaaatcat gacttgccat catacacggc ttccagttta
     9241 tcgtgagcgc ttagataatg tgattggttt tatccatgtc cgcaaaattt ttaatcaact
     9301 gaaaggggaa agaatcacag cgaacataat tgaacgagtg ttgcaagagc cctattttat
     9361 cccttctggg acacgcttgt tttcgcagct acagttattt caggaaaatc gaaaacggat
     9421 tggttttgtc gttgatgagt atggtgaatg gctaggtctt gtcacattag aagatatcat
     9481 tgaagagatt atcggcgagt ttacaacgca tgcgcccacg caaacgagcg catttattca
     9541 ggaaccagat ggtagtataa ttgctgaagg tagcgtgtta ttgcgtgatc ttaatcgcaa
     9601 acttggatat cagctgcctt tagatggacc taaaacttta aatggtttaa ttctagagca
     9661 ttttcaagat ataccggaag cagggatagg attgaatatc gcgggttatc caatggaaat
     9721 tatccaaacc aaagatcaag ccgttaagat tgttcgtatt cacccaacta ttcccaaaat
     9781 agtgggtgat aaaattgagt agattattcc ctaatcagcc acgcgcccat agctcagttg
     9841 gatagagtac cgccctccga aggcgggggt cgcacgttcg aatcgtgttg ggcgcgccat
     9901 atgacttacc tgcttttcaa aaaaataaac tagcgatgct cgatttgccg gtaccaagct
     9961 tcataaattt cgtcagacca caatgattta atccaaacaa gaatgtcgtc aatttcttgt
    10021 tcggatagct tatcttgcca ttcaggcatt cttccttcac caggaggccc tcctttcaag
    10081 atgaagcgct tcaattcttc agtagagtga tgccaagcat gcgcagtact gtcgagcggt
    10141 gggggcggat atctaccatt tgctaaaggt ttccgccaat cggctgttcc ttcaccattc
    10201 tgaccatggc aaactgcaca gttcttaata aaaatttcat ggccccgtgt aacttgttta
    10261 gcgtcaaaat cacgtttcac ttcgttcatc aaaccttgct cctggagctg ctggagtagt
    10321 ttcgttgcgg ttgtaacgga ttctgtacaa ctacttaaaa acaagatact agcgatcatt
    10381 atcgcctgat taaagcagcg ctgcaatttc attgataagc gatattaagg tctgtaacgg
    10441 ttgaacacag acgctttgtc acgcgtatcg cctttttctt gaatgagtag tacatcataa
    10501 ggatcgtttc gttccccttc catacctggc gtgccatgcg gcatatccgg aacggaaatt
    10561 ccaactgctt taggtcgttc tttaagcaat cgaataatgt cttgtgctgg tacgtgacct
    10621 tcgatcgcat aaccatcaac tagcgctgta tggcatgatc ctaatgttgg ggatatgcca
    10681 gcgcgcgctc ttgcttcttt atttccgaca tcatgcgtgt taacggtgaa cccatgatca
    10741 cgtaagtggt ccacccattt gccacagcag ccacaagttg ggctcttata aacttcgaca
    10801 gttcctgctg cactaacgaa attcgttgtg atgactaaat gaattgttag tattgatgta
    10861 agtatgaatt gtcttttgtt cgttttcatt tcttctccac ataaattttt ccacgcatcc
    10921 ctttaaagtg tccagggaaa gggcatgcaa aatctactat accagtttcc gtaaactgcc
    10981 agattaattc tttttgttct cctggcgcaa tttttatacg gttaggatcg ttatgcgcca
    11041 tattgggatg agcacgcatc attttggcgt gttcttttaa attttcaatg gtatcgatcg
    11101 caaactcatg atgttttttt ccatcgtttt ttacaaggaa gcgaatcgtt tcaccctgct
    11161 taatcgttat ctcatcgggt atgaagcggt tctcaatcat tttgatttcg atggtttgag
    11221 atacttgtgc agcatcgccg gactgtccaa tcagtgaatg ttcggggtga gtgcctgtat
    11281 ctgcaaatgt attcaatgtc caacttatca aaataaaata gagcatgaat agagattttt
    11341 tcatatagac cccctttgtt tttgagatga atcgactgtt acggctactt accttattta
    11401 gaatatagtc tatcctaaaa tgttccagtt ttgagttgta taatcttacc tatatcagca
    11461 actagcagaa ctgctataaa gatcgcacta gacgctatcg cacgatggat gatcactaaa
    11521 ggcttactta tgcaagaaat attatcaaag aagacacaat ataataacaa taagctacat
    11581 aaacgcttac gccgactggt tggaacagcg attgctgatt ttaatatgat tgagccggga
    11641 gatcggatca tggtttgttt atccggcgga aaagatagtt atggattatt ggatattttg
    11701 ttaaatttac aagcgcatgc tccgcttgat tttgaattgg ttgccgttaa tttggaccaa
    11761 aagcaacctg gttttccaga gcatgtctta cctgaatatt tagctcgaat tagtgttcct
    11821 tttcgtatcg tcgagcaaga tacttatagt gtagtaaagc gagtgattag cgagggcaaa
    11881 acaacctgta gcttatgttc tcgtttgcgt agaggtgttc tgtatcgtgt tgcgaaagag
    11941 cttggggcaa ctaagattgc actgggtcat catcgggatg atattcttga gacgtttttc
    12001 ctcaatctat tttatggcgg taagcttaag gcaatgccac caaaattagt tagcgatgat
    12061 gggaatcata tcgtgattcg tcccctcgct tattgtaagg aaaaggattt agcagcctac
    12121 gcacaggttg ccaattttcc aattattcct tgtaatctgt gcggttctca gcccaatttg
    12181 caacgacaag tcatcaagga aatgatgcag cagtgggata agaaatttcc gggtcgaata
    12241 gaaacaatgt ttaccgcgtt acagaatatc caaccttcac atttggctga tattgcgcgc
    12301 tatgattttc aggggcttaa ggcgcatgga aaagccgatg cagatggcga taaggcattt
    12361 gatgaagaac aatttgagcc tacgattgca gcgctaatgc cattttctga tgattcatcc
    12421 gtttatcagg ttgcctcgac acaggataat cataacggtt ttaaataaac taatggcggt
    12481 tatggtttct tttataagca atgggtggcg caagcttata agttatcaag acgagtagtg
    12541 caaataacag gtaaatactg gttaacacat attctggaaa atcataaaat aaaatccgat
    12601 gcaaccactg ttgcataaaa ctttgctctg atcctgttcc gcgtaattca tgctcccatg
    12661 ctgttaatgg gcaggtaata ccgatcagag attcaccgac gacaaacaag atggcggcga
    12721 ggtgtgttaa cctaaaagtg aagtttctga taaaaagcca gttgaaccaa gtacctagcc
    12781 aaataaccgg caggctagct actacgaata aaacaaaaac aaaatgaatt aacagaacga
    12841 tgtcagcagt gtgcacgtgc tggatgatta ttaaagtaac ggtttaatga ttttttcaat
    12901 ttcttcgatt ttgatgcgac ctaaatgact ataaacaatt tcacccgcac ggtttaagat
    12961 aacagtaaat gggagtgctc cctggggatt ccccattgcc tcggccaacg aaaatgcatc
    13021 aagctctcct acgacgacag ggtaattaat tccaaaatcg cgactgaagg aggcaactcg
    13081 atccggttga tctacagcga tcccaattaa aacaagccct ttatcgcgat atgtttcttg
    13141 ggcttgtata aactccggaa tttcttcctg gcacggtgca caccaggttg cccaaaagtt
    13201 taccagcaac acatttccct gccattgtga aaacggctgc atatcccctt taacatcagg
    13261 aagaccggaa gaaaagaact tttctacccc ccttttcttt tcatcgctac ttagttgggg
    13321 tgttagttgt tgtgccttga aaaaaaaacc agcagtcatt gcactgaagg caataacagc
    13381 atacagcacg attttgccaa attttgacat aagcttttat ccttgagaaa actgagaatg
    13441 cgctattaaa ttagattaat accgcgttta aaaccgctag aaaatcatct ttattgaggt
    13501 agccgataat tttgatattg ggtatttctg taccgttacg atctaaaaat aaaattcccg
    13561 gtgggccaaa tagcttaaag cgtttaagca aagcggcatc atccggcgtt ccatgggtaa
    13621 catcgacttg cagcagtact acatctttta attttgcttg tactcgctca tcagaaaagg
    13681 tgaatcgctc catttcttta caggagatgc accaatccgc gtaaaaatca accatgatgt
    13741 atttatcatg agactgtgca atgatttcgt ctagctcagt aactgatctg accggttgga
    13801 atggtaagtg ggctagttga tcccatccct tttcagagaa actaacattg ctggagttac
    13861 ttgtaatgct caattttgag agcggttgca aaacatctcg gcttccggat aggactccaa
    13921 tcagtaatgc aacacctgtc aataatgcga tgacaccaac acctttgaga aatttacgga
    13981 aacccaaggc attaacgggg agcggatcaa ttgcatgcag ataaattgcc gaaatgatga
    14041 gcaatgcagc ccatagcagc atgtaaatta ccgtggagac aacaggtgaa attaaccaaa
    14101 tcgcgacagc cagcaataga acaccaaaaa attgcttaat cgattccatc caagggccag
    14161 atttgggaag taatgcgccg gctgatgctc cgattaataa gagaggaacg cccattccaa
    14221 gcgccatgac aaataaagct gaccccccta ggacaatatc acgcgtctgg ctaatataga
    14281 gcaaagcgcc tgcgagcggt gctgcaacac aaggactgac tatcaaagcg gataatgcac
    14341 ccattccaaa aacactggat agatgaccac catggagatg gccggcttct tctgaaattt
    14401 tgctctgtaa gaagctggga agctgtaatt cataaaaacc aaacattgaa aaagcgagta
    14461 agataaatat cagggcaaag gtgcccaata cccaggcatt ttgtaacact gcagaaagca
    14521 ttgcgcccga caaaccagcc gccacaccgg caattgcata agcaatagcc attcctagca
    14581 cgtaagcaag ggcgagcacg aaacctcggc ctttagtcat atgctggctt cgattggcga
    14641 tgatcccaga taagatgggg aacattggaa atacgcaggg tgtaaaagaa agtaagagac
    14701 caataccaaa gaagccggcc agtattaggc caaaactgcc actggcaaac atttgttcaa
    14761 tcttgaaaga ttctgtctcg attgcgggtg gacgagaagg agtttgaaat aacgcagcag
    14821 tactatcaat gggtgccgcc gcggcattcc cactcaatgt ctcagcaaca gtatttacgg
    14881 ctgctttgat tgcaggtagc gttacctcat ttattttttt gatcggcgcg tagcagacac
    14941 caacgggttc attacaacct tgataggttg atgccagtga aagttgatct ggcgcctcaa
    15001 ttcctcgctt taatgcgatc actgcctgaa aaggctgata ataaacttct gttttaccaa
    15061 acgtgagatc atctttcatt ttaccaggcg gcaatgaaat tttttcaatc tgaattcctt
    15121 cactttgggg ttcgaatgcg attttatcgc gatagaggta ataattcttg gctggcgtaa
    15181 aatgagcgat tagcgtgtgc tcgtctttta cctcaatagc gagtttaaat gcttcgtctg
    15241 gcggtagaag ttcttgttct gcttgcccaa aattagcccc caatttttgt aaaagggtca
    15301 tgacacccga gcgaccctcc tcttgagcaa tcgccggatt aaccgtaaaa aagaaaagta
    15361 caaataaaaa tggaaataaa cgcataactt aattaattag taagtaataa atcaatttta
    15421 atgaacggtt tcatcagcaa tccattgtag ataagcaggc aaccctttta taatagggac
    15481 cgctatgatt tctgggagtt catagggatg caaggcttta atcgtctgct caaccgctgc
    15541 atagtgcgcg gataatgttt tgattactac cggcacctct gttgctgttt cggttttacc
    15601 cttccaatga tatattgaag tcaattgcga cccaatatga acacatgcag ctttatgttg
    15661 atcgatcagt tgcttagcta gccttaaagc ccctttttct tcgggaaagc tggtaagtat
    15721 cagtattgct tccataatat ctgttttgta caccgagaaa actagatttt ttcatttttt
    15781 tagtattaaa ctcaagcttt cttttaataa cataagatca gctagatctt ctagccattg
    15841 aaaaacgaat tgttttaaac gtgggttctt aatgatcaaa agactatttt tattattgca
    15901 gtttctcgcc ttcattgcgc cgattggtat ttttctaatg tatatcatca tggatgaagg
    15961 agatcaattt acctatgagc attactgggt aaccgcgatg agttttatcc cctttgtttt
    16021 cgtgttgctg attaaatata tgttttcaga tttagaccag aataaaaagg atgacagatg
    16081 aaaaagccta ttttattgat tggattattg atattaggga ttgttgaatg ggttcaaatt
    16141 tctgcaagct caaataccga acatgaaata ttttcagctg aagcagtaga aaaaattacg
    16201 attgatgccg gttttggttg ggggattttt ggtgggagtg tttataatgg taattcggat
    16261 tacacgataa ctcagattac cgttaaattg acgccatccc aaattattgg gacatcaagc
    16321 acatcgccat tagtagcaga gcgatatcat atcgatatta cagtccctcc tttaacgaaa
    16381 gctgcgcttt caatgccact cgatgcggat ggcacactag agtttctgtg ggagttggta
    16441 caagttcaag gttatagggt ttcagtgaag tcaaattagt ttcttgtttt ttcttagccg
    16501 attataatag ctactttgca taaattagtg atggagtatc tatgatcgtt aggcaaaaca
    16561 ttgttttgat tatcgttgca ttgtttactt tttctaactc ggcagtagct accaatcaaa
    16621 gtgctgagga aaaaaatcaa accagcttaa taactgactc agcgcagcaa gcagttcaac
    16681 cttcaaagca ctcaattctc aaagagaaaa aatcggagca gcctgaatca acaaaaccaa
    16741 tcaagcctaa tatggcggat tattgccgaa aacatacttg ctaattggta cggagtattg
    16801 attacatgaa attaatgacg aatttgagta attatatttt agctgcctcg cttacccttt
    16861 cgatcgccaa tgccgcagag tggtcttacg aggaacagaa acgatggggt agcattgaag
    16921 atcataacca gcacgaagta cctcatattt atccctatgc ggtttgtagc atcggtcagc
    16981 atcaatcgcc agtagatcta gcgacatcac aggtttctaa gcggctgaat aacttgaagt
    17041 ttcgttatta tgctgacaag cctgattttt ttaatacggg tcacgcggtg caggtcaatc
    17101 tttcaaatga ttatcaagga gaattattag tcggtaaaga agcctatcct ttgattcagc
    17161 ttcattttca cgagcctagt gagcatgtaa ttggaaccaa aatatttcct gcagaattac
    17221 attttgttca tgttagggat gatggaaaaa ttgcagttct tggggtgctc cttgaaattg
    17281 gaaaagataa cccggtgttt cagactatct tggaaaatac gccaccccaa gggggagagc
    17341 tcaagcaaga tagtgacgtg tggcttagca taaccagctt gcttcctgaa aaaagggatg
    17401 agtattacac tttagctggt tctttaacga caccgccatg cagtgaaggc gtggattggt
    17461 atgttttaac ggataccacc gaaatatccg aagcgcaatt ggatcggctc aaaagtttct
    17521 tttctgggaa tgctcgacta aagcaagaac ttaacaatcg ggctattgct aagaagaaat
    17581 aactttccct gtgcggctac gctaagtcta atgaacatgc tgctgatgct ttgatgtgac
    17641 caaagcagta atttgctctc ctggaatagg cttgttaatc cagtaacctt ggaatttcgc
    17701 acaaccggca tttttgagta tctcaaattg ctgttgattt tcgacgcctt ctgctactac
    17761 atcaagccca aggtttttgc ccatattgat gatggttttt actaaagtta aatcgtcctg
    17821 gtcggtcagg atatctctga caaacatccg ctcaattttt aattgcgcta atttaaagcg
    17881 ctttaaatag cttagcgaag agtagcctgt tccaaaatca tcgatggata agcgaatgcc
    17941 gagtgtacgt aattcattaa tttttttaac ggtatcatca atatgattga tcaaggaaga
    18001 ctcggtgagc tcgagaatta ggcggctatg atcaatctgt gattgctcaa tttttcgttt
    18061 taatttttgc acaaaacctg attcctgaaa ttgaactgcg ctgatattta gtgataatgt
    18121 caaagattta agtgtgtcat gttgttgcca aaattgtaat tgtctaattc cttcatcgat
    18181 aatccaatca ccaatcggaa tgatgtaacc attattctcg atgagtgaaa gaaactcatt
    18241 ggggccgacg atgcctttgt cagggtgctg ccaacgtaac agtgcttcag cgccgacaat
    18301 ttgctggctt tgattaatta ataactgata atgaagtctg aactgatgct tatcaactgc
    18361 tatccaaagt tccttttcga gctgagtccg gcgctctaat tcttgctgca tatgaggatc
    18421 aaaaaagcgc agtgtatttc ttccggcaca tttggcttca tacatcgctt tatcagccca
    18481 cttcaaaata tcatccgctt tactatactg atcagaaaac atgcagatcc cgatgctgga
    18541 tgaaccacga tgacgaatat tattgactcg atattcttga tgaagccgac ttaatattga
    18601 ttccgctatc tgagtagtaa tgttcatggc gtgactgtca tcgctaccca gttgctcaag
    18661 catgacaaca aactcatctc cacccaatct tgcgaccgta tcagtttccc ggatacagct
    18721 tgtaatcctt ttcgcagctt caataagcaa cgcatcaccg gcatcgtgac catgcgtatc
    18781 attcagaaat ttaaaattgt cgagatcaat aaatagcagc gcattataag ttttattacg
    18841 ctggcaagca gtgacagtat gtcttaatcg atctaagaat aagcgtcgat tgggtaattt
    18901 tgttaacgca tcataaaatg caagatcaag aatttccttt tctgaagctt tacgttgact
    18961 aatatcttct ttaatcgcga tgtaatagca aatttcatca gattcattgg taacaggcgt
    19021 tatagtcatg tgttcatggt acaaggcccc atcttttttc ttattgatta tttcgccttt
    19081 ccaaactttt tttgatgaaa ttgtctgcca catatcctta tagaataact cgctttgttg
    19141 tccagaattc atcaaatctt ttacgttcat accaacggct tcgtgacgac gatatcctgt
    19201 caattcggta aaagcaggat taatccattc aatggttgca gcagtatctg tgatcattat
    19261 ggcatttgct gcagcctcta aagcgatatt ttgaatctta agctgaagtt cagattgctt
    19321 gcgttcggta agatcgacat gagttccgat taatcgttta ggataaataa ggttaccgct
    19381 attatcacgc gccaatgatg ctcttgagag aacggtgacc cagtgccccc ctttatgctt
    19441 cattttaaac tcagtttcaa atttatcttt gcgccctgtg acataatcat caactaactt
    19501 gagaacccgg gatttatctt ctgggttgac taatttttcc caagttttaa ggcttggcga
    19561 tagtaattca cgatcggaat agcccagcat ggctttccac cggctagagt aataaacata
    19621 attggtttcc atattccaat cccaatatcc atcattagct ccgctgagtg cccag
//
LOCUS       DAOQLJ010000024        19610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_967_length_19610_cov_6.189926, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000024 DAOQLJ010000000
VERSION     DAOQLJ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 19610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19610
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_967_length_19610_cov_6.189926"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /gene="lnt"
                     /locus_tag="PK522_03175"
     CDS             complement(<1..1288)
                     /gene="lnt"
                     /locus_tag="PK522_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNC40768.1"
                     /translation="MDLKSKSFLALILGTLTVLGFAPFYYFPVPVITILLLFHLWRSS
                     TQSWHSAVIGFSFGMGLFGAGVTWLYVSLHDFGGMPLPIAVVALLLLCAYLSLFPALT
                     GWFATKIRHIHFSAWAACVSACWVMSEWLRGTLFTGFPWLTLGYSQVPTSPLIGYAPI
                     LGVYGISLLLVFSIALYIAWPKQKNLNHYLWILGGIWVSGYGLQHYQWVDPEGSPITV
                     SLLQGNISQDLKWREDHLVSTMNTYADLVLESNSRLIVTPEISIPLFYEDVPTDYLTL
                     LSDHAKQNQADVLIGFAETVPHSIDRNEYYNSMFSMGSSPEQRYRKHHLVPFGEFIPF
                     KPLFGWIIQVLNIPLSDFSRGSLDQQPLDLAGQKIAINICYEDVFGEEIILQLPQASI
                     LVNVSNDAWFGRSIGPRQHLQISQMRALETGRYMLRA"
     gene            complement(1351..1728)
                     /locus_tag="PK522_03180"
     CDS             complement(1351..1728)
                     /locus_tag="PK522_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6164 family protein"
                     /protein_id="HNC40769.1"
                     /translation="MSKLLFKLKNVPDDEADDVRQLLVDHQIDYYETTAGNWGISMPA
                     IWITHDGQFGEAKQLFDAYQTQRALNVRAEYAQLKKEGKHETLLGRVKENPLLYFIYT
                     LAVILLLYLPYKLILEIAEKLQN"
     gene            complement(1857..2762)
                     /locus_tag="PK522_03185"
     CDS             complement(1857..2762)
                     /locus_tag="PK522_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNC40770.1"
                     /translation="MQQQRYDQEFNFFLIISMVFSFGLVLFTSPVAAHDRAKALEQEV
                     LELKKRLISIQDELSQIRTDSSRVEQQVIESKQAIKEERVIVQQKIESEKEVIKKAIL
                     EESPRNMIFFRGGWARQDHHHNGISVQSSVAPVGAQEQAPQNAWYVGAGFDFGLTKDT
                     WGLIPKTELIAELMFEYKQFGTVLGNVLANEPTQLAGGDLNPRHVTSSTVTVSAAPKI
                     KFLEGYRLRPWIIPAGLAIHIISPPSETITFFQPAVMFAAGADYKLWKNIVLGVDARY
                     HLMGGHLDGIRLNGLTLGGYLGIGF"
     gene            3007..3873
                     /locus_tag="PK522_03190"
     CDS             3007..3873
                     /locus_tag="PK522_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC40771.1"
                     /translation="MNEWMDKVGEMNKSILITGCSTGIGYVTAHKLRERGYRVFATAR
                     RAESVAMLQAEGFESLQLDLDDSNSIQQAFAEIMQRTGNELFALFNNGAYGIPGAVED
                     LSRAALRAQFETNVMGWLELTNLVLPIMRNQGYGRIIQNSSVLGFAAMPFRGAYNASK
                     FAIEGLTDTLRLELMGTQIYVCLIEPGPISTQFRANALKALQKFIPVEQSVHREKYQA
                     VLSRLNKPGPAVPFTQPPEAVAKRVVEALEAKVPQPRYYVTFPTYLFGFLKRVLSIRL
                     LDRILAKAGTQN"
     gene            complement(4128..5045)
                     /locus_tag="PK522_03195"
     CDS             complement(4128..5045)
                     /locus_tag="PK522_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNC40772.1"
                     /translation="MKSFRHKSGFVLTTIAGVGLSIGLTMAAPQVFAHDRAKAMEQQI
                     KILESQLKTLREDFYRVQDSTNTEKREVEALKNEIRQEKEALQKAHAEHHEIEKAHDE
                     FGRTVARRDRVLFFRGGYAHNLQGRNGSSIQSNVAPVGVQESGDNNGWYVGAGLDWSL
                     TRDVWGLIPKTNVYAELMFEYKKFGVSQGGALANVPTTLVNAPDNPRGVTISQFTVSA
                     APKIKFMEGSDFRPWIIPAGFAVHVVSPTTESVTYLAPGMMFGAGFDYRIWKEFFLGA
                     DARYHETFGKTDGVNVSGLTAGAYLGIGF"
     gene            5158..5862
                     /gene="aat"
                     /locus_tag="PK522_03200"
     CDS             5158..5862
                     /gene="aat"
                     /locus_tag="PK522_03200"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNC40773.1"
                     /translation="MIHWLATDQVENFPPLNTALLEPNGLLAVGGDLSPQRLISAYRS
                     GIFPWFNDEDPILWWSPDPRMVLFPNELSISRSLHKLLKKHHYEVRTDASFREVMQAC
                     AGPRKTQAGTWITNEMIDAYTTLHQMGIAHSVETWMNGVLVGGLYGVALGKMFYGESM
                     FSRVSDASKIAFVHLVKQLEIWGFEMIDCQMKTQHLASLGAREISRNEFALRLSKLID
                     ISDRSGTWHFDYTYPG"
     gene            5878..6600
                     /locus_tag="PK522_03205"
     CDS             5878..6600
                     /locus_tag="PK522_03205"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HNC40774.1"
                     /translation="MKLHVTESYPCSYLPDQLARSEIAVPDSRVVDTQAYAKLIQEGF
                     RRSGYFVYRPNCEHCTACVPVRIVVDQFVPNRSQRRAWKRHHTLRVSLHKLHFHPDHF
                     RLYRRYQQKRHALSEMSCDTHDQYCEFLLQSSIDSVLVTFHDGNRLQMVSIIDELPDG
                     LSSVYTFFDPEIPQASLGTFSILWQIHYCRLEKHPYLYLGYWIEESRKMNYKAHFQPL
                     QYLRQGCWSRTAEPILKNRSVS"
     gene            6651..7676
                     /locus_tag="PK522_03210"
     CDS             6651..7676
                     /locus_tag="PK522_03210"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041353331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNC40775.1"
                     /translation="MLYRLLRPLLFTLDAELAHSLTLKALRLLQPFGLSDNRVVPCES
                     KTIMGLKFPNPVGLAAGLDKNGDYIDVLAKLGFGFIEVGTVTPRPQPGNPKPRLFRIP
                     EAHAIINRMGFNNLGVDYLLENIKRTQYHGIIGINIGKNFDTPVESAIEDYLLCFRKV
                     YAVASYVTINISSPNTLNLRQLQNEHALGHLVATLKLEQSKLSDQQGRYTPLLVKIAP
                     DLDESQLESIAAILMKHNIDGVIATNTTISRPNIAGLSHADETGGLSGSPVREASTRV
                     ISKLYSLLGEQIPIIGVGGIVSANDAKEKIAAGASLIQLYTGLIYAGPDLVNQCISTL
                     CHSQHVE"
     gene            7666..8346
                     /locus_tag="PK522_03215"
     CDS             7666..8346
                     /locus_tag="PK522_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNC40776.1"
                     /translation="MSSNENDSLIRQIDAILPQTQCGQCGYSGCLPYATAMAQGVADI
                     NQCPPGDETVIHQLAVLLEVSYKPLNRKYGIPKPKAVALIDEEYCIGCTFCIQACPVD
                     AIIGAAKCMHTVLTEQCTGCERCIAPCPVDCIRMVPIALTKRDEDPTLQRLAADKARE
                     RYQNRLRRLESERQRKNQLKSKSSTKVAQLASVDENLKKTMILAALERAKALSTVSLD
                     SNRGESEV"
     gene            8343..9002
                     /gene="nth"
                     /locus_tag="PK522_03220"
     CDS             8343..9002
                     /gene="nth"
                     /locus_tag="PK522_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNC40777.1"
                     /translation="MNAAKRQEMFSRLKSLNPHPTTELVYSTPFELLVAVILSAQATD
                     KSVNLATQKLFLVANTPAKILALGEVRLSEMIQRIGLYRTKAKNILATCKLLNELHAG
                     QVPRNREDLEQLPGVGRKTANVVLNTAFGEPTIAVDTHIFRVANRTGLAPGKNVLEVE
                     NKLLKRVPAPFQHDAHHWLILHGRYTCKARKPACHECCISDLCEFDEKNLYLTKRDLE
                     S"
     gene            9005..9436
                     /locus_tag="PK522_03225"
     CDS             9005..9436
                     /locus_tag="PK522_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HNC40778.1"
                     /translation="MFNPTRNQARQLFFDTWYKYGRHELLSGMETIALEVILQHTEYH
                     PILEDYDQFIDKDYLPEFGETNPFLHMSMHMAIKEQLSINQPVGIQERYRNLVVKLGD
                     EHAAIHNVMDCLGEMLWHAQRDQTSPNAQIYLDCLDKAIRS"
     gene            complement(9499..11346)
                     /gene="glmS"
                     /locus_tag="PK522_03230"
     CDS             complement(9499..11346)
                     /gene="glmS"
                     /locus_tag="PK522_03230"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNC40779.1"
                     /translation="MCGIVGAVAKDNVVPILLEGLSRLEYRGYDSAGIVVANAELQRL
                     RTTERVSELGRLVATKKTQGLTGIAHTRWATHGAPSERNAHPHFSGQDKKIAVVHNGI
                     IENHEQIRKRLEKEGFEFLSDTDTEVIAHLISFNLETTPNLLEAVCKTINELQGAYAI
                     AVVEESQPDHIVVARNGAPLLLGVGNNGNYVASDASALIQVTKHMIYLEEGDVAELQS
                     NGYRVLNRIGNAAYKEVTRLVRESDLTRDSVELGPYAHYMQKEIFEQPIAVANTLEMV
                     LNAHSISPQLFGTEAEAIFKKTNGILILACGTSYHAGLVAKYWLETIAGLPCNVEIAS
                     EYRYRDPIANPDCLVIGISQSGETADTLAAINYAKTLGHKHSLSICNVPESALIRQTN
                     LRFLTRAGPEIGVASTKAFTTQLAALMLLTVTLAKLRNRFSGELEQEVITSLRHLPIA
                     IQSALSVEPQVKAWAANFSQKRHALFLGRGVHYPIALEGALKLKEISYIHAEAYAAGE
                     LKHGPLALVDKEMPVIAIAPNDALLEKLKSNLQEVRARGGELYVFADSDSHIQESEGI
                     HIIRLAEHAGMLSPLLHTIPLQLLAYHVALQKGTDVDKPRNLAKAVTVE"
     gene            complement(11394..12767)
                     /gene="glmU"
                     /locus_tag="PK522_03235"
     CDS             complement(11394..12767)
                     /gene="glmU"
                     /locus_tag="PK522_03235"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNC40780.1"
                     /translation="MSDLDIVILAAGVGKRMRSSLPKVLHKLAGKPLLKHAIDTAQTL
                     SPRKIYIVYGHGGEQVLTEINDDQLTWVKQEPQLGTGHALLQVLPFLAKSGKTLVLLG
                     DVPLIQENSLTQLVASASANNFALLTVTLANPFGYGRIIREDGTNQVSAIVEEKDANE
                     IQRSINEINTGIMVLPNAYLHDYLPKLSNSNAQGEYYLTDIIKLAASSGIKIETAQPH
                     YEWEVSGINSKVQLAQLERAAQNFQAQQLLDAGVTLMDPSRIDIRGNLICGNNVEIDV
                     NCIFEGTVTLGDNIKINANCIIRDTIISSGTTVLPFSLIDNAEIGKNCHIGPYARIRP
                     GTRLDDNVHIGNFVEIKNSYIAMDSKANHLSYIGDATIGKKVNIGAGTITCNYDGAYK
                     HQTIIEDNVFVGSDTQLIAPVKVAKGSTIGAGSTITRDTPEEQLTLSRAKQISVAGWK
                     RPSKTKV"
     gene            complement(12922..13359)
                     /gene="nudB"
                     /locus_tag="PK522_03240"
     CDS             complement(12922..13359)
                     /gene="nudB"
                     /locus_tag="PK522_03240"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HNC40781.1"
                     /translation="MHAYKLPVSVLVVVYTIDRDVLLLERADHPGYWQSITGSQDPDE
                     SLWQTAVREIKEETGLVANRFELNDWHIQNQYEIFQEWRWRYAPGVTHNTEHVFGLRL
                     PEIMPISIASREHLSLIWLPWHEAAEKVFSWSNAEAIRKLDTT"
     gene            complement(13372..15153)
                     /gene="aspS"
                     /locus_tag="PK522_03245"
     CDS             complement(13372..15153)
                     /gene="aspS"
                     /locus_tag="PK522_03245"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNC40782.1"
                     /translation="MRTNYCGAIDKTYLDQEITLYGWVHRRRDHGGVIFIDLRDREGI
                     VQIVCDPDHKENFQIAEKIRTEYVLKIVGLVRHRPAGTTNSGIFSGEIEVLARAIEIL
                     NASLTPPFQMDEENLSEAIRLEYRYLDLRRPSMQQNIRLRHRVSMAIRTFLDQAGFID
                     VETPILTKSTPEGARDYLVPSRVHDGHFFALPQSPQLFKQLLMVSGFDRYYQITRCFR
                     DEDLRADRQPEFTQVDIETSFLSENEIMDLMEKMIRHLFLNAIDIDLPNPFPRMTFAE
                     AMHKYGSDKPDLRVPLVLTELTDLMQEVTFQVFRDAAQKSGGRVAALRIPQGGELSRK
                     EIDDYTQFVAIYGAKGLAYIKVNDRNKGLEGLQSPILKFLPETVIQAILERTQAQNGD
                     LIFFGADKAKIVNDALGALRVKIGHERGLAEKNWKPLWVIDFPMFEWDEEEKRWKALH
                     HPFTSPIEGHENLLTNDPEKALSRAYDMVLNGVEIGGGSVRIHRQEVQAKVFHALGIT
                     EHEAQEKFGFLLNALQYGAPPHGGIAFGLDRIVAMMTGTESIRDVIAFPKTQRAQCML
                     TQAPNSVTEKQLRELHIRLRNPSASIS"
     gene            complement(15317..15613)
                     /locus_tag="PK522_03250"
     CDS             complement(15317..15613)
                     /locus_tag="PK522_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNC40783.1"
                     /translation="MPIYEYICRSCGFEKEHLQKMSDAPIAQCPECGSHEYVKLVSAA
                     GFQLKGSGWYVTDFRNKPTSKSETQTKPNTSAGDNTTTTSEKAATPTSSTGTAE"
     gene            complement(15753..17213)
                     /locus_tag="PK522_03255"
     CDS             complement(15753..17213)
                     /locus_tag="PK522_03255"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HNC40784.1"
                     /translation="MHSLIPVILSGGSGTRLWPLSREKYPKQLLPLINEDSLLQATIK
                     RLDGLTGITVENPLIVCNEEYRFVIAEQLRLIGKACSILLEPFGRNTAPALTLAALVA
                     AQNNADPILLVMPSDHVIVNASAFQACISKGVSSALQNSIVTFGIAPDAPETGYGYIQ
                     TGELLGDVGITQIARFVEKPNQQTAQAYIDDGSYLWNSGIFMVRASVWLAAINQCRPD
                     IFSTCQTAWNKGSHEGNFIRVNETAFTACPNDSIDYAVMEKLSSNHPSLPSGVVIEFS
                     GGWSDVGAWDSLWQVLPKDEAGNVSRGDVILHECKNTLAISENRLVACVGVENLTVVE
                     TPDAILVVHKNRTQDVKKIVDSLKLTKRVEVILHRKIFRPWGWYDAVDAGERFQVKRI
                     VVKPGAALSLQMHHHRAEHWIVVRGTAKVTRDDKSYLVSENESTYIPLGTRHRLENPG
                     RLPLEMIEVQSGSYLGEDDIVRFEDIYGRNEAYKPN"
     gene            complement(17267..18387)
                     /gene="wecB"
                     /locus_tag="PK522_03260"
                     /pseudo
     CDS             complement(17267..18387)
                     /gene="wecB"
                     /locus_tag="PK522_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
     gene            18715..18909
                     /locus_tag="PK522_03265"
     CDS             18715..18909
                     /locus_tag="PK522_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNC40785.1"
                     /translation="MYICICKGVTDHAIREAVHQGAERMRDLKTCLGVTEQCGICACH
                     AKMVLDQALIQQQIGSASST"
     gene            18950..19414
                     /gene="bfr"
                     /locus_tag="PK522_03270"
     CDS             18950..19414
                     /gene="bfr"
                     /locus_tag="PK522_03270"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNC40786.1"
                     /translation="MRGNSDIITWLNRQLQHELTAINQYFLHARMYKNWGFNKLAEHE
                     YNESIEEMKHADQLIERILFLEGLPNLQELGKLLIGENAQECVSCDLKLELISRETLV
                     AGIAACETSQDYISREIFEKILEDTEEHIDWLETQLDVMQKVGMQNWLQSQI"
BASE COUNT         5481 a         4104 c         4246 g         5779 t
ORIGIN      
        1 ttgcacgaag catataacgc cctgtctcca atgcgcgcat ttgagaaatt tgtaggtgtt
       61 gccgcgggcc aatcgagcgg ccaaaccagg catcattact gacattcact aatatcgaag
      121 cttgtggaag ttgtaaaatg atttcctcac cgaatacatc ttcatagcaa atattaatgg
      181 ctattttttg cccagccaaa tctaatggtt gttgatcgag cgaaccacga gagaaatcgg
      241 ataaaggaat atttaatacc tgaatgatcc aaccaaacag cggtttaaaa ggaataaatt
      301 ccccaaaagg gactaaatga tgtttccggt accgttgttc aggagaacta cccatgctaa
      361 acatcgagtt gtaatattca ttacgatcga tcgaatgggg caccgtttct gcaaagccaa
      421 ttaaaacatc ggcttgattt tgctttgcat gatcactcaa tagcgttaaa taatccgtag
      481 gcacatcttc ataaaataat ggaattgata tttcgggggt gacgatcaag cgactgttgc
      541 tttcaagtac caagtctgca taagtattca tcgtgctaac aaggtgatct tctcgccatt
      601 tgagatcttg cgagatatta ccttgcagta agctaacggt tatcgggcta ccttcaggat
      661 cgacccactg ataatgttgt aatccatagc cactgaccca tattccaccc aatatccata
      721 gataatggtt tagattcttt tgttttggcc aagcgatata taaagcaatg ctgaatacca
      781 ataataacga aataccgtaa acgccaagga taggcgcata accaattaag gggcttgtgg
      841 ggacttgtga ataacctaga gtcaaccaag gaaatcctgt gaatagcgtc cctcttagcc
      901 attcagacat gacccagcac gctgaaacac aggctgccca agcgctaaaa tgaatgtgtc
      961 ggatttttgt agcaaaccaa cccgttaatg cagggaagag cgataaatag gcgcaaagta
     1021 acagcagcgc tacgaccgcg atcggtaagg gcatgccccc aaagtcatgc aggctaacgt
     1081 aaagccaggt tacaccagct ccaaataatc ccataccaaa cgagaaaccg atgactgctg
     1141 aatgccagga ttgagtgctt gaacgccata gatgaaataa tagtaaaata gtaatgactg
     1201 gcactgggaa ataataaaaa ggtgcaaatc ccaagacggt cagcgtaccg agaatgagtg
     1261 ccagaaagct ttttgatttt aggtcgattt ctttagatga atacacggaa tgattatgaa
     1321 tattgttgaa ctggctcgag atagcatcgt ttaattctgc aatttttctg caatctcaag
     1381 aatgagttta tagggcaagt aaagtaatag tatgacagct aaggtataaa taaaatatag
     1441 cagtggattt tcttttaccc taccaagtag cgtttcatgt ttaccttctt tttttaattg
     1501 agcatattca gctcgcacgt tcaaagcgcg ttgcgtttgg taagcatcaa ataattgttt
     1561 cgcttcaccg aattgcccat catgggttat ccaaattgca ggcatggaga taccccaatt
     1621 tccggcagta gtttcataat aatcaatttg atgatctacc agtaattgac ggacatcatc
     1681 cgcttcatca tctgggacat ttttgagttt aaacagtagt tttgacatcg cttagcatta
     1741 tgatcattac aatgcgatgc tagagtgaag ctccgtttaa tgtcaagtca gtcttatcta
     1801 aagaaagcgc tatccccacc agtgaagcgg gggtagcatg caagtcatcg taaatattag
     1861 aaaccgatcc ctaaatatcc acctagtgtt agaccattta gccggatacc atccaagtga
     1921 ccgcccatca aatggtagcg cgcatcaaca cctaacacga tatttttcca tagtttgtaa
     1981 tccgcgccgg ctgcaaacat aacagcaggc tggaagaaag tgatggtttc ggagggaggg
     2041 ctgatgatat gaattgctaa acctgccgga atgatccacg gtcttaaccg gtaaccttct
     2101 aagaatttga ttttaggtgc tgcggatacg gtgacagtac tcgatgtcac atggcgtgga
     2161 ttcaaatcac caccggcaag ctgagtcggc tcattagcca gcacatttcc caatacggtt
     2221 ccgaattgtt tatattcaaa catcaattcc gcgatgagtt cagttttagg aatgagtccc
     2281 catgtgtctt tggttaagcc aaagtcaaat ccagcgccaa cataccaagc attctggggc
     2341 gcttgttctt gtgctccgac cggtgcaaca ctgctttgca ccgaaatacc attatggtga
     2401 tgatcttgtc gtgcccaacc gccacggaaa aaaatcatat ttcgtggtga ttcttccaga
     2461 atcgctttct tgatcacttc cttctcggat tctatttttt gttgaacgat taccctttct
     2521 tctttaatcg cttgtttgct ttcgataact tgttgttcaa ctcgtgacga gtccgtgcgg
     2581 atttggctca actcatcttg gatagagatt aatcgttttt ttagttctag aacctcttgc
     2641 tctaatgctt tggcccgatc gtgtgccgct accggtgatg taaataggac taagccaaaa
     2701 ctgaacacca tactgatgat taagaaaaaa ttgaattctt ggtcgtagcg ttgttgctgc
     2761 attttttctc ctttgttaat aatgattaat gttttttact tataactccc tcagctcagc
     2821 agaaaaaatc aataatctgg atattaaatc tgttgaggtt aatcaaaggc tcagttactc
     2881 cagcatcgag atctctcggt ttatggtatt aaacgttttt ccaggaacaa cacgatgcta
     2941 tgatggtact tgctgtgaca aaaaactgag cgatgcccat cttaggggta agggtataaa
     3001 gaaatcgtga atgaatggat ggataaagtg ggtgaaatga ataaatcgat attgataaca
     3061 ggttgttcaa ccggcattgg ttacgtgact gcccataaac ttcgtgaacg cggctaccgt
     3121 gttttcgcga cagcgcgtcg tgctgaaagt gtagctatgt tgcaagcgga aggttttgaa
     3181 agcctgcagc ttgatttaga tgattcaaac tcgattcaac aggcgtttgc agaaatcatg
     3241 caacgcacag gaaatgaatt atttgcatta tttaataacg gggcctatgg aataccgggg
     3301 gctgtagaag atttaagccg ggctgcctta agagcgcagt ttgaaacgaa tgtgatgggt
     3361 tggctggaat tgaccaattt ggtgttgcca atcatgcgca accaggggta tgggcgtatc
     3421 attcagaata gttcagtgct gggatttgca gcgatgccat ttcggggtgc ctataacgct
     3481 tcgaagtttg cgatcgaagg actgaccgat acattgcgat tggaattgat gggcactcaa
     3541 atctatgttt gtttaatcga gccgggccct atctccacgc aatttcgggc aaatgcttta
     3601 aaagcgctac aaaaatttat cccagtcgag caaagtgtgc atcgtgaaaa ataccaagca
     3661 gtgctcagtc gattaaataa accagggcct gctgttccct ttacacaacc ccctgaagca
     3721 gtagcgaaac gtgttgtaga agcattggaa gcaaaagtgc cgcagcctcg ctattacgtg
     3781 acgtttccta cttatttatt tggttttctt aagcgagtgc tcagcatacg gttactcgat
     3841 cgcattcttg ccaaagcggg gacacagaat tgatcattcg aaattaacgc aaaaaaaaag
     3901 caccttactt gttaaagtaa ggtgcgaagg cttcgtcaca aaatcacagc ttgtcacaat
     3961 gaccttgtta gaaacaagag aagaaaaata caactaactt acactttcac taactactac
     4021 accaactaac aacaaccaac aatcaactac aacagagact ttaatcttat attcttatgt
     4081 atcgattaat taatctgcct ataaaacttt aaaaaccata ctactgctta gaaaccaatt
     4141 ccaagatatg caccggcagt taaaccactg acattgactc catcggtttt accaaaggtt
     4201 tcatggtaac gcgcatcagc acctaagaag aattctttcc aaatgcggta atcaaaccct
     4261 gcgccaaaca tcattcccgg cgctaaataa gtaactgact cggtagtcgg acttactaca
     4321 tgcactgcaa aacccgccgg gatgatccat ggcctgaaat cactgccttc catgaatttg
     4381 attttaggcg ctgctgaaac cgtaaattga ctaatcgtca caccacgagg attatccggc
     4441 gcattgacta atgttgtggg aacattggct agcgcaccac cttgtgaaac gccaaatttt
     4501 ttgtattcaa acatcaattc cgcataaaca ttggttttag gaatgagacc ccacacgtca
     4561 cgagttaaac tccaatccag acctgcaccc acataccaac cattattgtc gcctgattct
     4621 tgaaccccta ctggagccac gttgctttgt attgaagagc catttctacc ttgtaggtta
     4681 tgcgcatagc cgcccctgaa gaataataca cgatctcttc gcgcgaccgt tcttccaaat
     4741 tcatcatgcg ccttctctat ttcatgatgc tcagcatgag ctttctgtaa tgcttctttc
     4801 tcttgccgaa tttcattttt gagcgcttcc acctcgcgtt tctcagtatt ggtcgaatct
     4861 tgaacccgat agaagtcctc acgtagcgtc tttagctggc tctcaagtat tttgatttgc
     4921 tgctccattg ctttcgcacg gtcatgggca aacacttgcg gtgccgccat cgttaaccct
     4981 atacttagcc ctacgcctgc aatcgtggtt agtacaaatc ctgatttgtg acgaaatgat
     5041 ttcattaatt ctcctttctc tttttatttt ctataaactc atgccaatga tagaaaaaca
     5101 ttgcttaata aactttgatt taaatcaagc tttcttcatt cagtttaaat aaagttgatg
     5161 atccactggt tagcgaccga tcaggttgaa aattttccac cactgaatac agcgttgctt
     5221 gagcccaatg gtttattggc ggtaggtggc gatttatcac cgcagcgttt aattagcgct
     5281 tatcgcagtg gtatttttcc gtggtttaat gatgaagatc caattttgtg gtggagtccc
     5341 gatcctagga tggtgttgtt tcccaatgag ctctctattt cgcgatcgtt gcacaaatta
     5401 ctcaagaagc atcactatga ggttcgcacg gatgctagct ttcgtgaggt gatgcaagcg
     5461 tgtgctggac cacgcaaaac tcaagcaggg acatggatta ccaatgaaat gattgatgct
     5521 tatacgacgt tgcatcaaat ggggattgcg cattccgttg aaacgtggat gaatggtgta
     5581 ttagtcggtg ggctttatgg cgttgccctt ggaaaaatgt tttatggaga atcgatgttt
     5641 tcgcgagtga gtgatgcgtc caaaatcgct tttgtacacc tggtaaaaca acttgagatt
     5701 tggggatttg aaatgatcga ttgccaaatg aaaacccagc acctcgcatc tttaggtgcc
     5761 cgagaaattt cccgtaacga atttgcactg cgattaagta agctgatcga tatttcggat
     5821 cgatcaggga catggcattt tgattatact tatcctggtt gatcgttttt tcaaataatg
     5881 aagctccacg ttacagagtc ctatccctgc agttatctgc cagaccagtt ggcacgctct
     5941 gaaatcgcgg ttccagatag tcgggtagtc gatacacagg cttatgcgaa gttgattcaa
     6001 gaggggtttc gtcgcagtgg gtattttgtt tatcgaccca attgcgagca ctgtacggcc
     6061 tgtgtgccgg tgcgcattgt cgtcgatcaa tttgtgccta accgctctca gcgtcgcgct
     6121 tggaaacgcc atcatacttt acgggtgagc ttgcataagt tgcatttcca ccctgaccat
     6181 ttccggctct atcgtcgtta tcagcaaaag cgccatgccc tcagtgaaat gagctgtgat
     6241 actcatgatc aatattgtga gtttttgttg cagagtagta ttgactccgt gcttgtaaca
     6301 tttcatgatg gaaaccgact gcagatggtc agtatcattg atgagttacc cgatggttta
     6361 tcttcagttt atactttttt tgatccagaa ataccccagg caagcttggg tacttttagc
     6421 attttgtggc agattcacta ttgccggtta gaaaagcacc cttatcttta tttaggctat
     6481 tggatcgagg aaagccgtaa aatgaattac aaagcgcatt ttcagccgtt gcaatatttg
     6541 cgacaagggt gctggtcacg tacggctgaa cctatattga agaatcgatc tgtatcttaa
     6601 ttatcatgat ggtccgctaa attttcgctt gaacccagga aatcatcttc atgctttatc
     6661 gtttgttgcg ccctttgctc tttacactcg atgctgaatt agcgcatagc cttacgctta
     6721 aagcattgcg tttattgcag ccattcgggt tatcagataa tcgggtggtt ccctgtgagt
     6781 ctaaaaccat tatgggactc aaattcccta atccagtggg gcttgcggct ggtctggata
     6841 agaatgggga ttatatcgat gtgctggcta aactggggtt tggctttatt gaagttggca
     6901 cagtaacgcc gcgaccccaa cctggcaatc caaaacctcg gctatttcgc atcccagaag
     6961 cgcacgctat catcaatcgt atgggcttta acaaccttgg tgtcgattat ttgttagaga
     7021 atataaagcg cactcaatat catggaatta tcggtatcaa tatcggaaaa aattttgata
     7081 ccccggttga aagcgccata gaagattatt tgctttgctt tcgtaaggta tatgcggttg
     7141 ctagctatgt aacgatcaat atttcttccc ctaatacact gaatttgagg caattgcaaa
     7201 atgagcatgc gttaggtcat ttagttgcta cgttaaaatt agagcaaagt aagctcagtg
     7261 atcagcaggg gcgctacacg ccgctattgg tgaaaatagc ccctgacttg gatgaatcac
     7321 aacttgaatc gattgctgct atcttaatga aacataatat tgacggtgtt attgccacca
     7381 acactacgat ttcgcgtccg aacatcgcag ggttatcgca tgcggatgaa accgggggat
     7441 tgagtggatc cccagtgagg gaggcgagta cccgagtcat tagtaaattg tattcgctgc
     7501 taggggagca gattccgatc attggtgtcg gaggtattgt gtcggccaat gatgccaaag
     7561 aaaaaatagc agcgggcgca agtctcattc aactctatac gggtttgatc tatgcaggcc
     7621 cggatttggt aaaccagtgc atcagtacac tttgtcattc gcaacatgtc gagtaacgaa
     7681 aacgattcat taatcagaca aatcgatgcg attttgccgc agacacaatg tggacaatgt
     7741 ggttattcag gttgcctgcc ttatgcaacg gcaatggcgc aaggtgttgc ggatattaat
     7801 caatgtcctc ccggtgatga gacagttatt catcaattgg ctgtgttatt agaggtttcc
     7861 tataaaccac tgaatagaaa gtatggtata ccgaagccta aagcggttgc tttgatcgat
     7921 gaagaatact gtattggttg cactttttgc attcaagctt gtcccgtgga tgcaatcatt
     7981 ggagcagcta aatgcatgca taccgttctt actgagcaat gtactgggtg tgagcgttgt
     8041 atcgcgcctt gccctgtaga ctgtatccgg atggttccca ttgcgctgac taaacgagat
     8101 gaggatccaa cattgcagcg attggcggct gataaagcca gagagcggta tcaaaatcga
     8161 ttgcgacgat tagagtccga acggcagcgc aagaatcagc ttaaaagtaa atcatcgacc
     8221 aaggtggctc aattagcctc agtcgatgag aatctaaaga aaacaatgat acttgcggca
     8281 ttagaacgtg ctaaggcctt atcgactgtt agcctggaca gcaatcgggg tgagtctgaa
     8341 gtatgaatgc tgcgaagcgc caagaaatgt ttagccgatt gaagtcgtta aacccccacc
     8401 caacgacaga gttggtttat tcaacaccat tcgaattatt agtcgcggtc attctttcgg
     8461 cgcaagcaac agataaaagt gtcaatcttg caacgcaaaa actttttctc gtggcaaata
     8521 ccccagcaaa aattcttgca ctgggtgagg tgcgattaag tgaaatgatc cagcggattg
     8581 gattatatcg aaccaaagca aaaaatatcc tagcgacgtg taagttgtta aatgagttac
     8641 atgcgggtca agtaccgcga aatcgagaag accttgagca gcttcctggg gttggccgta
     8701 aaacggcgaa tgtggtgctg aatactgcat ttggagaacc aaccattgca gtagatactc
     8761 atattttcag ggtcgcgaac cgaactggac tagcaccagg aaaaaatgtg ctggaagtgg
     8821 agaataagtt actgaagcga gtgcctgctc ctttccaaca cgatgcccat cattggttga
     8881 ttttacatgg gcgatatacg tgtaaggcca ggaagcctgc ttgccacgaa tgctgcatca
     8941 gcgatttatg cgaatttgac gagaaaaatt tatatttaac gaagcgagac cttgaatcgt
     9001 agcaatgttt aatcccactc gaaatcaagc ccggcaatta ttctttgaca cttggtataa
     9061 atatggccga catgagctat tatccggaat ggaaacaatt gcactggaag tgatattaca
     9121 acacacagaa tatcatccga tactcgaaga ttacgatcaa ttcatcgaca aggactatct
     9181 accggagttt ggagagacaa atccgttctt gcacatgagc atgcatatgg caatcaaaga
     9241 acagctttct atcaatcaac cggttggtat ccaggaaaga tatcgcaatc tagtcgtgaa
     9301 attaggcgat gaacatgcgg caatccataa cgtaatggat tgcttgggtg agatgctttg
     9361 gcatgcgcaa cgggatcaaa cgagccctaa tgcgcagatt tatttagatt gccttgataa
     9421 agccattagg tcttaagaaa gtactagtcc aaaatatgaa ctaaaaaaac cattacatag
     9481 aactgatcag ccacatgatt attcaacagt aactgctttt gctaagttcc ttggcttatc
     9541 gacatcggtc cctttctgta aagcaacatg gtacgctaat aactgtagtg gaatggtatg
     9601 taacagggga cttaacatac ctgcatgctc cgcaagccgg ataatatgaa tcccttcact
     9661 ttcttgaata tgcgaatcgc tatcagcaaa cacatagagt tcgccaccgc gtgcgcgaac
     9721 ttcttgtaag ttggatttaa gtttttctaa taatgcatca ttgggtgcga tggcaatgac
     9781 gggcatttcc ttatcaacta acgctagcgg gccatgtttt aattcaccag ctgcataagc
     9841 ctctgcatga atataagaaa tttcttttaa tttaagtgcg ccttctaatg caatgggata
     9901 gtgaacgccg cggcccagaa atagcgcatg tcgtttttgt gaaaaattgg cggcccatgc
     9961 tttaacttgg ggttcgacag ataacgcgga ttgaatggca attgggaggt ggcgtaatga
    10021 agtaatgact tcctgctcta gctctccaga aaaccgattt ctaagctttg cgagtgtcac
    10081 cgttaatagc atgagtgctg ctaactgcgt tgtaaatgct tttgtagaag ccacaccaat
    10141 ttcagggccg gcacgtgtta aaaagcgtaa attggtttgt ctgatcaaag cgctttctgg
    10201 aacattacaa atcgacaagc tatgtttatg tccaagagtt tttgcgtaat tgatcgcggc
    10261 aagcgtgtct gccgtttccc ctgattggga aataccgatg accaggcaat ctgggtttgc
    10321 aatgggatcg cgataacggt actcactggc aatttcaaca ttacaaggta acccagcgat
    10381 cgtttctagc cagtatttgg caactaagcc tgcatgatag ctcgtcccgc aggcaagaat
    10441 tagaatacca ttggttttct tgaaaatggc ttctgcttca gtcccaaaga gttgaggtga
    10501 aatagaatgg gcgtttaata ccatttccag ggtattagca accgcgatcg gttgctcaaa
    10561 gatttctttt tgcatgtaat gggcgtaagg ccctaattca acagaatcac gcgttaaatc
    10621 gctttctcgg actaaacgtg ttacttcttt ataggcagcg ttgccaatac ggttaagaac
    10681 gcggtaacca ttgctctgta attctgctac atcgccttct tcaaggtaaa tcatatgttt
    10741 agtcacttga atgagcgcag aggcatccga agcgacatag tttccattgt taccgacacc
    10801 taataatagc ggtgcgccat ttctggctac gacgatgtga tctggttgcg attcttccac
    10861 aaccgcaatt gcatacgcac cttgtagctc attgattgtc ttacatactg cttcaagcag
    10921 atttggggtt gtttcgaggt tgaaggatat gaggtgagca atcacttcag tatccgtatc
    10981 ggataggaac tcgaaacctt ctttttcaag gcgcttgcgg atttgctcat gattttcgat
    11041 gataccattg tgcacgaccg caattttttt atcttgccct gaaaaatgtg ggtgagcatt
    11101 tctttctgaa ggtgcaccat gcgtcgccca ccgtgtatgt gcaattccgg taagtccttg
    11161 ggtttttttg gttgcaacga gtcttccaag ttctgaaacg cgttcggtag tgcgtaatct
    11221 ttgtaattct gcatttgcaa cgacaatgcc agccgagtca tagcctctat attcgagcct
    11281 agaaagacct tctagcagaa taggaaccac attatctttc gcgactgcgc ctacaattcc
    11341 acacatgctt ttctccttcc aattgcttcg atttaccagg acaatgagta ttattaaact
    11401 ttagttttac ttgggcgctt ccaccctgca acgctgattt gtttagcgcg agacagtgtg
    11461 agttgttctt caggcgtgtc tcgcgtgatc gtggagcccg caccaatcgt agagccttta
    11521 gcaactttaa ccggtgcaat taactgcgta tccgagccaa caaaaacatt atcttcaatg
    11581 atcgtctgat gtttataggc accatcgtaa ttgcaagtaa ttgttcccgc gccaatattt
    11641 acttttttgc cgatagtcgc atcaccaata tagctaaggt gattcgcttt actgtccatt
    11701 gcaatgtaac tgttttttat ttcgacaaaa ttaccgatat ggacattatc atccaaccga
    11761 gtgcctggtc ttattcgcgc atagggtcca atatggcaat tttttccaat ttcagcatta
    11821 tctatcaggc taaacggaag aacagttgtt cctgaactga taatagtatc tctaatgatg
    11881 cagtttgcat tgatttttat attatctcct aatgtaactg taccttcaaa gatacagtta
    11941 acatcaattt caacattatt accacaaatt aggttgcctc taatatcaat acgagacgga
    12001 tccatgagtg ttactccagc gtctaatagt tgttgcgctt ggaaattttg tgctgcccgc
    12061 tcaagctggg cgagttgtac tttactattg ataccggata cttcccattc atagtgaggt
    12121 tgagcggttt caatcttaat tccagaactc gcagctaact taatgatatc cgtcagatag
    12181 tattcacctt gagcattgct attactaagt ttgggtaagt aatcatggag ataggcattg
    12241 ggaagtacca taataccggt attgatttca ttaatgctac gctgtatttc gttcgcatcc
    12301 ttctcttcta caatggctga aacttgattg gttccatctt cacgaataat ccgcccataa
    12361 ccaaaaggat tggctaacgt gactgttagt aatgcaaaat tgttggcact tgcgcttgca
    12421 acaagctgag ttaatgaatt ttcttgtatt aaaggaacgt cacctagcag aactaaggtt
    12481 ttacctgatt ttgctaaaaa aggaagcact tgtagcaaag catggccggt gccaagctgt
    12541 ggttcttgct taacccatgt gagctgatca tcgttgattt cagtcagaac ctgttcgccc
    12601 ccatggccat atacgatata aatttttcta ggcgataatg tttgcgctgt gtcgattgca
    12661 tgcttgagta agggtttacc agctaattta tggagcacct ttggaagaga tgatcgcatg
    12721 cgtttgccaa ccccggcagc taatatgact atgtctaaat cactcacaat cgtttccttt
    12781 tcataatcct aaatatgata ttgcagcaat aactaatccc aaatcaagaa taagcgctaa
    12841 atttcaatat gttttgttta aatttgattt gtatgtgaca gacaaaggat gtaatgaatt
    12901 tgcaggtatt cattaaactg atcaagtggt atccaatttt ctgattgctt cagcattact
    12961 ccatgaaaat actttttcag cggcttcgtg ccagggtaac cagatcaatg agaggtgttc
    13021 gcgcgatgca atcgaaatag gcataatttc tggtaatctt aaaccaaaaa catgctcggt
    13081 attgtgagtg actccggggg cataacgcca acgccattct tgaaaaattt catactgatt
    13141 ttggatgtgc caatcgttta attcaaatcg attggcaact aatcccgtct cttctttaat
    13201 ttctcgaact gctgtttgcc aaagtgactc atccggatct tggcttccgg taatcgattg
    13261 ccaataaccg gggtgatctg cacgttccag tagaagaacg tcacgatcga ttgtatagac
    13321 gaccactaat actgatacgg gaagcttata ggcatgcact ataaggataa tttaagaaat
    13381 actagcgctt gggttacgga gtcgtatgtg taattcccgt aattgttttt cggtaacact
    13441 attcggtgct tgggttagca tacattgtgc gcgttgtgtt ttagggaaag cgatcacatc
    13501 acgaatggat tcggtcccgg tcatcatggc gacaatgcga tccaaaccaa atgcaatacc
    13561 cccatgtgga ggtgcgccgt attgaagtgc attgagtagg aatccaaatt tttcttgagc
    13621 ttcatgctct gttattccga gtgcgtggaa aacttttgct tgtacttctt ggcgatgaat
    13681 ccgtactgac ccgccaccaa tttcgactcc atttaatacc atatcatagg ctctcgagag
    13741 tgctttctca ggatcgttag tgaggagatt ctcatggcct tcaatggggg atgtgaacgg
    13801 atgatgtagg gctttccacc gtttttcttc ttcatcccat tcaaacattg ggaaatcaat
    13861 gacccatagg ggtttccaat ttttctctgc taatccacgc tcatgcccaa tttttacacg
    13921 caatgcgccg agcgcatcat tgacgatttt agctttgtcc gcgccaaaga aaatgagatc
    13981 cccattttgt gcttgcgtgc gttccaaaat tgcttgaatg actgtttctg gcaagaattt
    14041 tagaatcggt gattgcaaac cttcgagacc tttgtttcga tcgttgactt tgatatacgc
    14101 taagcctttt gcaccataaa tcgctacaaa ttgtgtatag tcatcaatct ctttacgcga
    14161 taattctccg ccctgtggaa ttcgaagggc cgcgacacgc ccacctgatt tttgggcagc
    14221 atcgcgaaat acttgaaaag tgacttcttg catcaggtcc gttaattcgg tgagcacaag
    14281 cggtacgcgt aaatcaggtt tatcggagcc atacttatgc atcgcttctg caaaagtcat
    14341 ccgtgggaaa gggttcggta ggtcaatatc gatagcgttc aggaataagt gacgaatcat
    14401 cttttccatt aaatccataa tctcattctc tgagagaaac gaggtttcga tatcgacttg
    14461 tgtaaattcg ggttggcgat cagcccttaa atcttcatca cgaaagcaac gggtaatctg
    14521 ataatagcga tcaaatccgg ataccattag cagttgctta aataactgag gtgattgggg
    14581 taaagcaaaa aagtgcccat catggacgcg tgaaggtact aaataatcac gtgcgccttc
    14641 tggggtcgat ttggttagga tgggtgtttc aacatcgatg aaaccagctt gatccagaaa
    14701 tgtgcggata gccattgaga cgcgatgcct tagccttata ttctgttgca tcgaggggcg
    14761 tcgcaagtca aggtagcgat actcaagccg aatggcttca ctgagatttt cttcatccat
    14821 ttggaaaggg ggggtgagag atgcattgag tatctcgatt gcgcgcgcta aaacttcgat
    14881 ttcacccgaa aaaattccag aattggttgt acctgctggt cgatgcctta ctaaccctac
    14941 gatcttaagg acatactcgg tacggatttt ctcagcgatt tgaaaatttt ctttgtgatc
    15001 aggatcgcat acgatttgca caatgccttc gcgatcacgt agatcgatga aaataacacc
    15061 accgtgatca cgacggcgat gcacccatcc ataaagtgta atttcctgat ctaaataggt
    15121 tttatcgatg gcaccgcaat agttagtccg catagatttt aattaggctc gaatagaaaa
    15181 aaggggtaca ggataatcgc gtgcaattct acagaaattt tgcgtgctca gactgtatcg
    15241 atattaatgt catggattca ataaaaatta aaactgattt aggcgagttc tcgttataaa
    15301 catcagttgt attgtactac tctgcagttc cggtagaaga agtcggggtt gcagcttttt
    15361 cagatgtggt agtagtatta tccccagcac tcgtattggg ttttgtttga gtctcggatt
    15421 tagaagtggg tttattccta aaatctgtaa cataccaacc acttcctttt aattggaacc
    15481 ctgctgctga tactaattta acgtattcat gactaccaca ttctggacat tgtgcaattg
    15541 gtgcatcact cattttctgt aagtgctctt tttcaaaacc gcaggaacgg caaatatatt
    15601 cataaattgg catgactgat ctccattacg cgacagaatg aaatggaaaa aaacgcatat
    15661 tatacttcaa cccatttgtc ctttcgttct taaatctgta aataaaatcg atttgtagat
    15721 ttgaattaga gaagcagcat ttctctgttt ttttagttag gtttataagc ttcatttcta
    15781 ccataaatgt cttcgaaccg gacaatatcg tcctcaccta agtaggatcc tgattgaact
    15841 tcaatcattt ctagtgggag tcgacctgga ttttccagac gatggcgtgt tcccagcggg
    15901 atatacgttg attcgttttc actcactaag taacttttat cgtcccgtgt tacctttgcc
    15961 gttccacgga caacgatcca atgttctgca cgatgatgat gcatctgaag ggataaggca
    16021 gcaccaggct tgaccacgat acgcttaact tgaaagcgtt ctccagcatc cacggcatca
    16081 taccaacccc aaggtcggaa aattttccgg tgtaatataa cttctacgcg ttttgttagt
    16141 ttaagcgagt cgacaatttt cttgacgtcc tgtgttctat ttttatgaac cactaagatt
    16201 gcatccggtg tttcgacgac agttaagttt tcaacaccta cgcaagccac taaacgattt
    16261 tctgaaatag cgagtgtatt tttgcactcg tgcaaaataa catctccgcg tgaaacgttg
    16321 cccgcttcgt ctttaggaag cacttgccaa agggaatccc atgccccaac atcagaccaa
    16381 cctcctgaga attcgatcac gacaccggat ggtaatgaag ggtgattgct tgatagcttt
    16441 tccatgaccg cataatcgat ggaatcattg ggacaagcag taaatgctgt ttcattgaca
    16501 cgaatgaaat tcccttcgtg agaaccttta ttccatgctg tttggcatgt tgaaaagata
    16561 tctgggcgac attgattaat cgcggctaac caaactgatg cgcggaccat gaatatcccg
    16621 ctgttccata gataagagcc atcgtcgata taggcttggg ctgtttgttg gtttggtttt
    16681 tcaacgaagc gggcgatttg agttattcct acgtctccaa gcaactcacc tgtttgaatg
    16741 tagccataac cagtttctgg ggcatcggga gcaatcccaa acgtgacaat cgaattttgt
    16801 aatgcactgg atacgccttt gctaatacat gcttgaaacg ctgaagcgtt aacgatgaca
    16861 tgatcggatg gcatgaccag taaaatcgga tctgcattgt tttgtgcagc gactaaggct
    16921 gcaagagtga gtgctggagc agtgtttcgt ccaaatggct ctaataaaat tgaacatgct
    16981 tttccgatta gcctgagttg ttcagcaatt acaaaacggt actcctcatt gcaaacgatc
    17041 agtggatttt caacggttat gcctgttaaa ccatctaaac gcttgatggt ggcttgtaac
    17101 aatgagtctt cattgatcaa gggcaataat tgttttggat acttttcgcg cgagagaggc
    17161 cacagtcgag ttccagagcc accagataaa ataacaggaa tcaatgagtg catagttttt
    17221 gtacaaaaaa attataaaaa atatttttac tgcaatgaat gttcttttag acccgctgca
    17281 agagcttctt ctcaagaatc tcaacaattc gttcggcagc gtgaccatcc catagttgag
    17341 gtcgatgacc ttgtttacct tttccttgca gaaggttatc tacttcttga atgattcgct
    17401 cgggattggt tccaactaag gtattcgtcc cttcatccac agtaatagga cgttccgtat
    17461 tttcacgaat ggtaatgcaa ggaatcccta gtgcagtggt ttcttcttgt aagccgccgc
    17521 tatcggttag aactactgca gcgtttttcc ataaatcaag aaatggcata taggcttgag
    17581 ggcctactaa cgtgatgttg ctaccaaggt caataccaaa ttttttaata ttttcccgag
    17641 ttcttggatg agtaggaaaa atgagcggta aatgggtgga aatttctttt agcgcttttc
    17701 cgattcgaga gaaaacttct ctatcatcaa cattactcgg tcgatgaagt gtaataacac
    17761 cgtagcgctg cttgctcaga gtccggtcta gcttaaattt gcttatttca gattcttcga
    17821 gattgatatg cgttaattta ctggcttggt atagcaaatt atccaccatc acatggccaa
    17881 caaagaaaat ggcgtcatca gctttgcctt cacgttttag atgttctacc ccactgggtt
    17941 cagttacaaa aaaccaatct gagatgctat cagtaacgag tcgattgatt tcttctggca
    18001 ttgttaaatc accgctacga agcccggcct cgacatgcgc taccgggata tgtaatttct
    18061 tggctacaat ggagcaagca agcgtagaat tgacatctcc caccacaatg accgcatcgg
    18121 gtctatcgtt aaggcaatat tcttcaaaag ctagcattat tttggctgtt tgctgggaat
    18181 gactgcctcc tccagcggcc ataaaatatc gggttctgga atgccgagtt cttcaaagaa
    18241 tacttcgttc atttctcgat cataatgttg gccagtatga acaatgcggt aggctaaagt
    18301 ctctttctct tggagcgcac ggatgatagg tgcaattttc atgaaattgg gccgtgcacc
    18361 ggctacaaga taaatttttt tttgcatgat tgattctgcc ttaatggaaa ttgagtattg
    18421 gggatcgcat ggaaatagcg taagtcgcat tcagagtatc aaagaaggca tgaaccatgc
    18481 agtaaactat atttcattac gctatgacct gtttaatcct tcgataagga aatcgagatt
    18541 aattctaaac tatctcgcta tttatggtag agatgaattg aagtaggtat ttttagttaa
    18601 tcttatttct gaatttatag atagatggca aatcagttac ttgcactatt tgaaatggtg
    18661 caatataatt acgaatcatt ctcattaaca atttttggtt tataggttta actcatgtat
    18721 atttgtatct gtaagggtgt tacggaccat gcgattcgcg aagctgtgca ccaaggagcg
    18781 gagcgtatgc gggatttaaa aacgtgtttg ggcgttaccg aacagtgcgg aatttgtgca
    18841 tgccatgcta aaatggtatt ggatcaggca ttgatacagc agcaaattgg ctcggcctct
    18901 tccacctaaa ttgatgtgct tcacaagtta aacgctctag gaggtaataa tgagaggtaa
    18961 ttccgatata atcacctggt taaatcgcca gctgcaacat gagttaacag caatcaatca
    19021 atattttctc catgcgcgca tgtataagaa ctggggcttt aataagcttg ctgagcatga
    19081 atacaatgaa tcgattgagg aaatgaagca tgcagatcaa ttgattgaga gaattttatt
    19141 tttagaaggt cttcccaatt tacaagaatt aggaaaatta ttaattggtg aaaacgcgca
    19201 agaatgtgtt tcgtgtgact tgaaactaga acttatttct cgtgaaacac tagtcgctgg
    19261 gatcgcagcc tgtgaaacaa gccaagatta tatttctcga gaaatttttg agaaaatctt
    19321 ggaagatact gaagagcata ttgactggct tgaaacgcaa ttggatgtga tgcaaaaggt
    19381 tggtatgcag aactggttac aaagtcaaat ctaattttct tacttaatgc tgctggccct
    19441 tcattctttg tcagcagcat tttcctaaag ctagctaaaa attctaacta taagcttggt
    19501 tgttattatt aaagcactct taaattgatc attctgcgtt gttcgacatg ttccaggcat
    19561 taagaaattt tttggttgaa aatttaatta agaatggtca atttatctct
//
LOCUS       DAOQLJ010000025        19554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_970_length_19554_cov_6.536027, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000025 DAOQLJ010000000
VERSION     DAOQLJ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 19554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19554
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_970_length_19554_cov_6.536027"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..1017
                     /locus_tag="PK522_03275"
     CDS             4..1017
                     /locus_tag="PK522_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNC40787.1"
                     /translation="MQLPGVDDRPLWDVIFAIYGYPALLLAHRLKIFSTLAKNTCTLS
                     EICNALNLKPRPVEAILTTATALGFLSLHEERYRLTPVTEVYLLENSPNYFGFFWDMM
                     IDNYQASSIASLEEAILTDKPQVYGGNNLFKTHEEQIELLRRFTAGMHSISITSALVW
                     PTIMDFSRHRIMLDIGGGSGAHSIGAAIKLPNLEQALIMDFPQVCEVAEGYIAQYKMQ
                     NRVKVHAANIWQDHWPMADLHFYSNMYHDWMPDKCHFLTAKSFGNLESGGRIVIHEML
                     YNDDKTGPFAIAAFSMIMMGWTEGKMYSGQELTKMLDEVGFEDIQIYKAFGYHSIVTG
                     VKP"
     gene            complement(1046..1330)
                     /locus_tag="PK522_03280"
     CDS             complement(1046..1330)
                     /locus_tag="PK522_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40788.1"
                     /translation="MKTFFTHDIDSSRENDAFDQWLIQMYACPEILSEVREQRLIEWE
                     KAPFARFCHPREEHLLPLQVCYGIACADSPIAKVMFNEQIMGKKVTSLLW"
     gene            complement(1293..1475)
                     /locus_tag="PK522_03285"
     CDS             complement(1293..1475)
                     /locus_tag="PK522_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40789.1"
                     /translation="MYPKAQIPCIQLLLLRNLDPEKHIALGKALASLREKNILIIGSG
                     VISSFENIFYPRYRQQ"
     gene            1585..2199
                     /locus_tag="PK522_03290"
     CDS             1585..2199
                     /locus_tag="PK522_03290"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNC40790.1"
                     /translation="MPNNKSVIFAVVSSFFSFTLISLSTPLKANDNLHAQSILVAEAH
                     QHDHKSNAQEKENNQPNSSHQHGQDKKNKHQHGMMEEKHDYAHMIIFHADKLKLTNEQ
                     LGKLVRLHLKHEEAHKKIKEKLKDSMHAFKKAKMDPSSTDEQLSSLGKDHANAFNAMV
                     EHHIQERRAIHNVLSAEQKNQLISTIKMNHSAHDDKGGGHSHDH"
     gene            2279..2572
                     /locus_tag="PK522_03295"
     CDS             2279..2572
                     /locus_tag="PK522_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40791.1"
                     /translation="MGRNHFDKELFELDERIGRLAIACGVDIAQKNTIVSLIKGNYSV
                     CLQGDNPRRQELRGLLMLKYHIEESCIDAIGANECVQLIDDEEARLRQRGFSI"
     gene            complement(2619..3686)
                     /locus_tag="PK522_03300"
     CDS             complement(2619..3686)
                     /locus_tag="PK522_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40792.1"
                     /translation="MKTKFFILKLITISLILNFPINAANAMPGMHGSEGKSHTEAPRV
                     SKSMGDKINTTGAELEITYSADGKTAIFVSTREGSIESPGSPYNFDIWISKNVDGEWQ
                     TPIHLGPDIDPTLGPNINTSAWELEPSFSDDGNVIYFTRYEPGNLLSGDLYVVQKIDG
                     VWQTAKNWNDVPELPNINTPTGEEHCPIIASDSLIYFSYHQPGVTQDSDIWKVEKKDG
                     VWQQPESLGAKINSPHRDHMHWTGLSKDGKSLIITSMRPDLGSRGGHDEWISHQDEKG
                     EWQVPVNLGDAVNTAGEDMCWTFTPDGKKFTGSWGPYSTYDMDIRWINKDDVPLLKNF
                     EPIGPPPNLLINRKSTQNTSQ"
     gene            complement(3785..5737)
                     /locus_tag="PK522_03305"
     CDS             complement(3785..5737)
                     /locus_tag="PK522_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC40793.1"
                     /translation="MDYFAKSRKIFTILLIEKLAFKLLIVIRFPMLDIQHLTYLQNGL
                     PLLSDVNFQVFANQRVGLVGKNGSGKSTLFRLIRGEIHPEKGEVNLQVGKTLAYVEQE
                     IINSAKPSLEFVLDGDSELRQLEAILKQDNHDAKWFDAQQRYELIDGYGARARAAQLL
                     DGLGFSNTEHDAPVSKFSGGWRMRLNLARALMHRADLLLLDEPTNHLDLEAIFWLEHY
                     LQNYPGSLIIISHDREFLNVCVNRIAHIEHQKIQSYSGNYDDFERARMERIAQHEQTY
                     EQQQKQIAHMEDFVRRFRAKATKAKQAQSRIKALERLTRVAPIQVENGYFSLQILAPD
                     NGPDTLLKIDSMGFGYTDESMLFQKVNLCLRTGDRIVLLGPNGVGKSTLIKLLTAELL
                     PAKGKVEINSSIRIGYFSQHQLEYLNGAQTPLQHLQQLAHNETTLTLRNFLGRFGLAD
                     DSKDRPIATFSGGEKSRLALALLAWQKPHLLLLDEPTNHLDLDMRDALIVALENYTGS
                     LVIVSHDRSLIRAVADELWVVADSNVHLFDGDLDDYKDWLEKRRDNGTDKAKPKKQSQ
                     TKRATANKRSMLTQQTKIEAALAGVQQELAALNLQLSDPAIYANASREAISELNLKRS
                     ELEKKIDALEANWLELESLMEEFGKV"
     gene            complement(6053..7189)
                     /gene="bamC"
                     /locus_tag="PK522_03310"
     CDS             complement(6053..7189)
                     /gene="bamC"
                     /locus_tag="PK522_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HNC40794.1"
                     /translation="MKRFSKLFGLLLAIIMLISGCHLLPESQKMEYKSAGKLPPLEIP
                     PDLNAPQADDRYAVPDMASTGSATYSSYNRDRDEQPRSQGSYVLPVAKDTARIARSGT
                     QRWLVVQLAPEDAWPVVKEFWQDMGFLLKVEAPDVGIMETDWAENRAKIPQDLIRGLL
                     STFLDSIYSSAERDKFRTRFERSESGSTEIYISHRGMDEVFVDRSTNRTAWQPRSADP
                     NLEAEMLTRLMMRFGVEEESARKEVAANENIITQDQAYLDKTRHGVLIVSETFDRAWR
                     RIGLALDRGGFTVEDRDRSNGVYFVRYVSPEYESRKTSDDKGLLTKLAFWQKSQDDSR
                     TEKFRIQVDGVGASSEVKVLTLEGAPEESAAADRILKLLYEQLK"
     gene            complement(7210..8088)
                     /gene="dapA"
                     /locus_tag="PK522_03315"
     CDS             complement(7210..8088)
                     /gene="dapA"
                     /locus_tag="PK522_03315"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNC40795.1"
                     /translation="MFKGSLVAIVTPMHDDGALDLEKFCELIDFHIAEKSDGIVVVGT
                     TGESPTVDFEEHHLLIKTAVSHAAGRIPIIAGTGANSTREAIELTQFAKNAGAAACLS
                     VVPYYNKPTQEGMYQHFKTIAEAVDIPMILYNVPGRTVADISNQTVVKLASVPNIIGI
                     KDATGDIGRGCDLLSQIPENFSVYSGDDATSLALLLLGAHGVISVTANVAPKLMHEMC
                     AAAFSSDLANARAINDKLLKLHTDLFVEANPIPVKWAVSRMGLMGNGIRLPLTPLSDQ
                     YHSLLIAAMKQAEVLN"
     gene            complement(8149..10749)
                     /gene="clpB"
                     /locus_tag="PK522_03320"
     CDS             complement(8149..10749)
                     /gene="clpB"
                     /locus_tag="PK522_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNC40796.1"
                     /translation="MRLDKFTTKFQQALGDAQSIALGKDHPYIEPQHLLLALLQQEDG
                     STVSLLQRAGINVNPLREGLAKNLERMPKIEGGGGEINISRELNNLLNLTDKEAQKHG
                     DQFIASEMFLLALLEDKGETGLLLKQHGASRSALEQAIKSVRGGEQVSSAEAEGGREA
                     LKKYTLDLTERARAGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPDTLKNKRVLSLDMAALLAGAKYRGEFEERLKAVLKELSQDEGRTIMF
                     IDELHTMVGAGKAEGAIDAGNMLKPALARGELHCVGATTLDEYRKYVEKDAALERRFQ
                     KVLVDEPTVEATIAILRGLQERYELHHGVDITDPAIVAAAELSHRYITDRFLPDKAID
                     LIDEAAARIRMERDSKPEVMDKLDRRLIQLKIEREAMKKEKDEASQKRLALLEDEIKK
                     LEREYADLEEILKSEKMRAQGSQQIKEELEKLRKEVEVATRKGDWQKVSELQYGRIPK
                     LEAQLAEQVQHAEATLPERELNKPKLFRTQVGAEEIAEVVSRATGIPVAKMMQGEREK
                     LLLMEQKLHERVVGQDEATRLISDAIRRSRSGLSDPNRPYGSFLFLGPTGVGKTELCK
                     ALASFLFDSEDHLIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEMVRRKPYSVI
                     LLDEVEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSQMIQQMSGDD
                     YQIIKLAVMGEVKTYFRPEFINRIDEVIVFHALDAKHIQSIAKIQLRNLENRLAKMEI
                     HLEISEAALAKIAEAGFDPVFGARPLKRAIQTQIENPLAKELLEGHFAAKDTIHVDCE
                     DETITFTKSS"
     gene            complement(10863..11696)
                     /locus_tag="PK522_03325"
     CDS             complement(10863..11696)
                     /locus_tag="PK522_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNC40797.1"
                     /translation="MKTSLFDLAASCLIANEIEEKLLLTQQATQAWRNKLLSLESHEP
                     MRLINQPGQPAKLVVVKPSDVPRRRLGTKLGLIALIHAIAHIEFNAINLAWDAIHRFR
                     GLPDSFYDDWVTVASEEAYHFQLLHKRLKELDHDYGDLPAHNGLWDMALLTASDPLIR
                     MALVPRVLEARGLDVTPGIIKRLRQIGDEKTIEILEIILCDEIGHVSIGSRWYNFFCN
                     QRQIDPEKKFSELVNQYFGGQITGPFHYEARQKAGFTLNELSMLEGMEIPISRSNNCN
                     F"
     gene            11978..12973
                     /locus_tag="PK522_03330"
     CDS             11978..12973
                     /locus_tag="PK522_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HNC40798.1"
                     /translation="MPAKQNIDHSKSPLNVIISVDVELWPDNWDLGHTEFNRCLNKFI
                     HGKTTSGNYGLGFQLAQLKEHNLKCVFFVESLFSGHYGIGPLQEIVAQIQEAGQEVQL
                     HLHTEWLGRGRFHNPLNKTGYNLFNFNQAEQQLLICQGKEFLAQAGADNLLAFRAGNF
                     GSNLQTLEAVPQAGFIFDSSINPRFYIKNGLDTPLHVEEYKEGIYEFPLTIFKEWGGR
                     LSQFQFGGSCSFKEMANLLGQAWINEWHSVVILSHGSELLNRAKTRPDKIVVDRFVQT
                     CRFLANNRDLFKTIWFSDIQPETIYAKSKESCVLQSGFINTAHRYLEQTTRRLYG"
     gene            12982..13947
                     /locus_tag="PK522_03335"
     CDS             12982..13947
                     /locus_tag="PK522_03335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC40799.1"
                     /translation="MSNNAVESVEFKFRIGEITLWTSHLKLNVFRNHFTEYEHPYTEE
                     SDYSDSIDEKLDGVLFRSRPITTLLPRFTLKQDVIRYAPKQYHRYFIDLTGSKEEYLE
                     KFSAKSRSTLKRKIKKFSDHCGNQIDWREYKTLAEMEIFYPLARQVSTKTYQERMLAA
                     GLPEDDLFIQRMYALAQKDSVRAYLLFCQDKPVAYLYCPISNGILFYQYLGYIPEMAQ
                     WSPGTVLQWVVIENLFLESHFAMFDFTEGPGKGSGGHKEFFSTNSTLCADIFFLRKTN
                     INLFLVLLHTKIDSFSAKIGIFLDRFGLKTKIKNFLRKLYPSASH"
     gene            14079..14762
                     /locus_tag="PK522_03340"
     CDS             14079..14762
                     /locus_tag="PK522_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNC40800.1"
                     /translation="MLYLLLIILVIALIIGPAYWVKHVMEKYSHPDNRYAHTGAEFAR
                     QLLDQESLSHVKVELTESGDHYDPLNKVVRLTSDKFNNASLTAITVAAHEVGHAIQDR
                     DDYLALKLRTKLVVLVAPAQKLGAAILMLAPVSVIFIRSPLVGGLMLAGGFLSLGSAA
                     LVHIITLPMELNASFSRALPLLKKSKYLKEGDELHAHKLLTAAAWTYVSASLMTLLNM
                     ARWWAILRR"
     regulatory      14821..15020
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            15147..17000
                     /gene="btuB"
                     /locus_tag="PK522_03345"
     CDS             15147..17000
                     /gene="btuB"
                     /locus_tag="PK522_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor"
                     /protein_id="HNC40801.1"
                     /translation="MRSCLLFGFFVALCSTGYVSANNVQPPVIVTATRTAQTADETLA
                     SATVITQADIARLQARSVEDLLRGVPGLNIVNNGGPGKQTSFFLRGTESDHVLVLIDG
                     VRVGSVTSGTTSFENIPIQQIDRIEIVRGPRSSLYGSEAIGGVIQIFTRKGGGQIKPN
                     FSFGGGSYNSINGSAGLAGGGNQGWFNINVSGIGTRGFNACTGSETAGCFMTEASRVD
                     DQDKDGYRNLASSLRAGYRFNQAFEVDAHFLHSAGDTQFDGGYANRSKLKQQVLGGSI
                     RYSPFSFWQLNLTAGRSFEKLDTYKIERSEQIRRSTFLSQFDSIRDSVFLQNNFSIGN
                     HQLLTAGIDYFNDQVKSTESYLETSRRNWGLFAQHQAAIAAHNLQLSMRWDHNEQFGK
                     WVTGGIGWGYAITQNLRLTANFGNAFKAPNFNELYFPFSGNPNLRPESSRSFEMGITS
                     TTKLGKWSVNAYETHINNLITFDIQTFSSINVDRARIQGLESVFNTQILNWTINANFT
                     LLNPENASSPDDKILPRRAKHIFRIDADRQFNHYTLGGTFLAEGKRFDDTENTRLLNG
                     FVRVDLRAEYAFAQNWRLQARLENLFNEHYETAAFFNQPGRNVFFTLRYQT"
     gene            17028..17930
                     /locus_tag="PK522_03350"
     CDS             17028..17930
                     /locus_tag="PK522_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNC40802.1"
                     /translation="MKNLLFDCNEFKVAHTGNVGFANLAQRPTNIKPIYSSKEHYQML
                     LLETQQEIGQLQEMLYAYNRYAILLIFQGMDSAGKGGVIKHVMSGVNPQGCQVFSFQQ
                     PSLEELDHDFLWRSNKCLPERGRIGIFDRSYYEEVIVVRVMPDLLKQQRLPLECADPK
                     KIWVERFQDIINLEHYLHRNGIQIIKFFLHLSKEEQRQRLIARIDEPSKNWKIGRADL
                     EAHKHWEAYQLAYDECIDATSTHHAPWYIIPADDKRNARLLVSHIIVETLQALKIAYP
                     ILSKDQLKELATIRQLLENEKPPN"
     gene            complement(17923..18456)
                     /locus_tag="PK522_03355"
     CDS             complement(17923..18456)
                     /locus_tag="PK522_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40803.1"
                     /translation="MNLLSWIKQIFSDQAGNQFGIRQKESKSLDFIFEQDDINCFKHL
                     CQFVWIQGEPIPLIFDLSNHVYVNQKITLPMIERLVAIGLVDYAPEGYVKKKLGKHTR
                     FFYHGRLTKIGFAQDAGNQLDLGVVLLTSSGKKLYATLSPIRNQQFYEYVIQHWFQKG
                     LLLSSPLGSPQRPHFVN"
     gene            complement(18495..18806)
                     /locus_tag="PK522_03360"
     CDS             complement(18495..18806)
                     /locus_tag="PK522_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40804.1"
                     /translation="MEIKKWTDEELASTRNKLEEWCEKQKKSGKVMWIVTALLGAFAV
                     STGAVFIFFDGITLLSVVLIILGVMTCFTWYQSEKRRKNNIDFLEEVKREITHRAKRD
                     K"
     gene            19110..>19554
                     /locus_tag="PK522_03365"
     CDS             19110..>19554
                     /locus_tag="PK522_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNC40805.1"
                     /translation="MNNKKIEKRVIQLYEKLELSNEIKLPEKDAAQLIRHGAHIIFQL
                     DTHNHFIYLNPAWETTTGLPVAKTVKLSLLNFVHEQDHDYLRKKLQLIRTSASKHSFH
                     TECRLKVNNTQIIHVEMQLIYSPENNKAQHVMGIMVDISKHKIFLE"
BASE COUNT         5794 a         4112 c         3725 g         5923 t
ORIGIN      
        1 aatattcaac taccgggggt agacgaccgt ccgttatggg atgtaatttt tgcgatttat
       61 ggttatcctg ctttacttct ggcacatcga ctcaaaattt tttctaccct ggctaagaat
      121 acatgcacat tatcggaaat ttgtaacgcg cttaacctta agccgcgccc tgttgaggca
      181 atattaacta cagcaactgc actgggattt ctatctctcc acgaggagcg ttacaggtta
      241 acgccggtta ccgaagtata tctactcgag aacagcccaa attacttcgg ttttttctgg
      301 gatatgatga tagataacta tcaggcatct tcgattgcaa gcttagaaga agctatcctc
      361 accgataaac cccaagtata tggtggtaat aatcttttta aaacacatga agagcagatc
      421 gaattactgc gccgttttac agcgggcatg cacagtatta gcatcacttc agcattggtt
      481 tggccgacca tcatggattt ttccagacac cgcatcatgc ttgatattgg cggcggctct
      541 ggcgcacatt ccataggcgc agcaataaaa ctgcccaatc tggagcaagc actaataatg
      601 gattttcccc aagtatgcga agtagccgaa ggatatattg ctcagtataa aatgcaaaat
      661 cgtgtcaagg ttcacgcagc taacatatgg caagatcact ggccaatggc tgatcttcat
      721 ttttactcaa acatgtatca tgactggatg ccagataaat gccatttttt gacagccaag
      781 agcttcggca accttgagtc aggtggaaga attgtgattc atgaaatgct ttacaacgac
      841 gacaaaactg ggccatttgc tattgccgcg ttcagtatga tcatgatggg ttggacagaa
      901 ggaaaaatgt attctggcca ggaattaaca aagatgctcg atgaagttgg atttgaagac
      961 attcagattt ataaagcatt tggttatcat agtattgtta caggtgttaa gccttagtac
     1021 tttcccatat gtgattctga ttctactacc acaacaagct cgtcactttc tttcccataa
     1081 tctgctcatt aaacataacc ttggctatgg gggagtcagc acaagcaata ccataacaaa
     1141 cctgtaaggg caataaatgc tcttccctcg gatgacaaaa ccgagcaaat ggtgcttttt
     1201 cccattcgat caatcgttgt tcgcgtactt cagataaaat ttccggacag gcgtacattt
     1261 gaattaacca ttgatcaaat gcatcatttt ctctactgct gtcgatatcg tgggtaaaaa
     1321 atgttttcaa atgatgaaat gacacctgat ccgataatca gaatattttt ctcacgtagc
     1381 gatgctaaag cttttccaag cgcgatatgc ttttccggat ccagattcct gagcagcaat
     1441 aattgaatac agggaatttg tgcttttgga tacatcagct tgagcggtac aatggctcta
     1501 ggctagcaga aaagatatgt tagtctagag ccatttatat tctagaactt tatgattcga
     1561 tccaacttaa ggagatacct taaaatgcca aataacaaat cggttatttt cgcggtagtt
     1621 agttcttttt tttcgtttac gttaataagt ttgagtactc ctttaaaagc aaatgacaat
     1681 ttgcatgctc aatctattct agtcgctgaa gctcatcagc acgatcataa aagtaacgct
     1741 caagaaaaag agaataatca gcctaactca agccatcaac atgggcagga caagaaaaat
     1801 aagcaccaac acggcatgat ggaagaaaaa catgactacg cgcatatgat cattttccat
     1861 gcagataagt taaaactaac taacgagcag cttggaaaac ttgttcgact ccacctaaaa
     1921 catgaagaag cgcataagaa aataaaggaa aaattaaaag atagtatgca cgctttcaag
     1981 aaagctaaga tggatcctag ttcaacagac gagcagcttt caagcttagg aaaagatcat
     2041 gcaaatgcat ttaacgcaat ggttgaacat catattcaag aacgtcgagc catacacaac
     2101 gttttatccg ccgagcaaaa aaatcaactc atcagcacga tcaagatgaa tcatagcgct
     2161 catgatgata aaggtggcgg acatagtcat gatcattaaa aatttactga tattgatgat
     2221 ttgtaattcg ctatcttcag tgtaaatgaa aaacctgggg gctgtctaag tatttcaaat
     2281 gggaagaaat cattttgaca aagaactttt tgagcttgat gagcggatcg gtaggcttgc
     2341 gattgcttgt ggggttgata tcgctcagaa aaatactatt gtttcactca ttaaaggaaa
     2401 ttactcagtt tgtcttcaag gtgataatcc aagacgccag gagcttcgtg gattattaat
     2461 gcttaaatat catattgaag aaagctgtat cgatgcaatt ggagcaaacg aatgcgtcca
     2521 attgatcgat gacgaagaag ccaggttaag acagcgtgga ttttctatat aaattcaact
     2581 tgattacaga aatcaaccca accaagatta aattagtgct actgacttgt attctgggtt
     2641 gatttccggt taatcagtaa attcggtgga gggccgattg gctcaaaatt tttcaataaa
     2701 ggaacatcat ctttgttgat ccagcgaata tccatatcat aagtactata cggaccccag
     2761 ctacccgtaa actttttacc atctggcgta aaagtccagc acatatcttc ccctgccgta
     2821 ttgactgcat caccaagatt gactggcact tgccattctc ccttttcatc ttgatgcgat
     2881 atccattcat cgtgccctcc tctcgaaccc aaatcaggcc gcatactggt gataatcaaa
     2941 cttttaccat cctttgataa tccagtccaa tgcatatggt cacgatgagg tgaattgatt
     3001 ttagcaccta aactttctgg ttgttgccat accccatcct tcttctctac tttccaaata
     3061 tcactatctt gcgtaacacc tggttgatga tagctaaaat aaatcagact atcggaagca
     3121 ataattggac aatgttcctc acctgttggg gtattaatat tgggtaactc tggcacgtca
     3181 ttccaatttt tagccgtttg ccaaacacca tcgatcttct gaactacata aagatcccct
     3241 gataataaat ttcccggctc atatcgcgtg aaataaatga catttccgtc atcagagaag
     3301 cttggttcaa gttcccaggc tgatgtattg atgttaggtc ctagtgtggg atcaatatca
     3361 ggtcctaaat gaataggtgt ttgccactcc ccatcaacat ttttagaaat ccagatatca
     3421 aaattataag gtgatccagg tgactcgata ctgccttcac gcgttgaaac aaaaattgct
     3481 gtcttgccat cagcactgta ggtgatctcg agttcggcac ctgtggtatt aattttatca
     3541 cccatactct tcgatacgcg cggagcttct gtatggcttt taccctccga cccatgcata
     3601 cctggcatcg catttgccgc atttatagga aaattaagga taagcgatat agtaatcagc
     3661 tttaatataa agaatttcgt tttcatagac tattttcctc ttcaagcaaa caataaataa
     3721 aacttgctca cgataagcat gagttaatgc gcaatatttt gcaaaaatct tcaatcggtg
     3781 agtatcatac tttaccaaat tcttccataa ggctttctag ttctaaccaa tttgcttcca
     3841 atgcatcgat ttttttttca agctcagaac gcttgagatt caattcactg atggcctctc
     3901 tcgaagcatt agcatagatt gcaggatcag aaagctggag attgagtgca gcaagctctt
     3961 gctgaactcc tgctagtgca gcttctattt tggtttgctg tgtaagcatt gaccttttat
     4021 tagcggtagc acgcttggtt tggctttgtt tttttggttt agctttatct gttccattat
     4081 cacgtcgttt ttcgagccaa tctttgtaat catccaaatc accatcaaat agatgcacat
     4141 tgctatcagc aacaacccat agctcatcag ctacagcgcg tattaagcta cgatcatgcg
     4201 atacgataac aaggctgccc gtataatttt ctaatgcgac gatcagcgca tctcgcatat
     4261 cgagatcgag atgattggta ggttcatcaa gaagtaacaa gtgtggtttc tgccaagcga
     4321 gcaaggctag cgccaaacgg cttttctctc caccagaaaa agtggcgata ggtctatctt
     4381 tgctgtcatc tgccaaccca aagcgaccta aaaagttacg cagcgtgagt gtcgtttcat
     4441 tgtgggctaa ttgttgtaga tgctgtaatg gcgtttgagc accattgaga tactcaagct
     4501 ggtgttgtga aaaataacca atgcgaattg aagaatttat ctctactttt cctttagcag
     4561 gtaataattc tgctgtaagc agtttaataa gtgtggactt tcccacgcca ttgggcccta
     4621 ataacacaat tcgatcaccc gttcttaagc ataaattcac cttttgaaat aacatgcttt
     4681 catcggtata tccaaatccc atgctatcaa ttttcaacaa cgtatcaggg ccattatccg
     4741 gcgcaaggat ttgtaaggag aaatatccat tttcaacttg gatgggcgca acccgtgtta
     4801 atctttctag cgctttaatt ctactttgtg cttgcttagc tttggttgct ttggctcgaa
     4861 aacgacgtac aaaatcttcc atatgtgcaa tttgtttctg ctgttgctca taagtttgtt
     4921 cgtgttgagc aatacgctcc atacgagcgc gttcaaaatc atcataattt ccgctgtaac
     4981 tttggatttt ctgatgctca atatgggcaa tgcgatttac gcaaacatta agaaattctc
     5041 ggtcatgcga aataatgatt aaactgccgg ggtaattctg caaatagtgt tctagccaaa
     5101 aaattgcttc cagatcgagg tgattagtag gttcgtctag caaaagcaaa tctgcacgat
     5161 gcatcaaggc acgtgctaaa tttaagcgca tacgccatcc ccctgaaaac ttgctaacag
     5221 gcgcatcatg ttcagtatta gaaaatccca acccatccaa caactgagct gcacgtgctc
     5281 tcgcaccgta accatcaatc aattcatacc gttgttgtgc atcaaaccat ttagcgtcat
     5341 gattatcttg cttaaggata gcttctagtt gacgcaattc actatctcca tctaatacga
     5401 attcaagtga tggtttagca gaattgataa tttcttgttc aacataagca agtgtttttc
     5461 ccacctgcag attaacttcc cctttctcag gatgaatttc tccacgtatc aatcgaaata
     5521 atgttgattt tccgctccca ttttttccaa ctaacccaac acgctgattt gcaaatactt
     5581 gaaaattgac atcactcaat aacgggagtc cattttgtaa ataagtcaga tgctggatat
     5641 cgagcatggg aaatctaatg acaataagta gcttaaatgc aagtttttcg attaataaaa
     5701 tcgtaaaaat ttttcttgac tttgcgaaat aatccacatt ttagatcgat taataaaaga
     5761 aagccttaga aacccgcgcc aatacaaagg atttttgtaa ccaactgttt ttaaacacgt
     5821 atttaattgt ataaaactta atcgaatcat aaaaaattat tacagataac tatcttagcg
     5881 cgaatactga cgaattattc acagacttat ccacagaaat tgtggacaat ggcaacagag
     5941 ctccaaacga aaaaattaag aagcgtaaca taaagaaata taccatcata acccaatgat
     6001 attagggtta atgtactgaa gatgtgttta gtcgaaaaaa aactaaatct tattatttga
     6061 gctgttcgta gagtaatttt agtattctgt cagccgctgc agattcttcg ggagcgcctt
     6121 ctaaagtaag tacctttact tcactacttg caccaacgcc atcaacctgg atgcgaaatt
     6181 tttcagttct agagtcatcc tgactttttt gccagaacgc aagtttagtt agcaagcctt
     6241 tatcgtcgct tgttttacgg ctttcatatt ctggactaac gtaccgaaca aaataaacac
     6301 cattggaccg atcacgatct tcgaccgtaa agccccctct atctaaagcc aatccaatgc
     6361 gacgccaagc acgatcaaac gtttcactaa caatcaacac accatgccta gttttatcta
     6421 aataagcttg gtcttgcgtg ataatatttt cgtttgcagc cacctctttt ctagcacttt
     6481 cttcttcgac tccaaaacgc atcatcaaac gcgtcaacat ttctgcctct aaattgggat
     6541 cggcggagcg tggttgccaa gcggtacgat tggtactccg atcaacaaaa acttcatcca
     6601 taccacgatg gctaatataa atttccgtac tgcccgattc acttctttca aatcgtgtac
     6661 gaaatttatc tcgctctgca ctggaataaa tactgtctag aaaagtcgaa agtaagcccc
     6721 gaatcaaatc ttgcggaatt tttgctcgat tctcagccca atcggtttcc ataataccga
     6781 catctggtgc ttcaactttc agcaaaaagc ccatatcttg ccaaaattct ttgacaaccg
     6841 gccatgcatc ttctggagcc agctgcacaa ccaaccaacg ttgcgttcct gatcgagcaa
     6901 tacgtgcagt atctttcgca acgggtaaaa cataactccc ttgtgatcgg ggttgttcat
     6961 cacgatctct gttataggaa gagtaggtag cactcccggt tgaggccata tctggaactg
     7021 catagcgatc atccgcttgc ggcgcattta aatcaggtgg aatttccaat ggtggtaatt
     7081 ttcctgctga tttgtattcc atcttttgac tttcaggcaa taaatgacaa cctgaaatca
     7141 acatgataat cgctagtaac agtccaaata atttactgaa tcgcttcatt ttgcattaca
     7201 aatttattat taattcagga cttccgcctg tttcattgct gcaataagca atgaatggta
     7261 ttgatccgaa agtggggtca agggtaaccg gattccattg cccatcaaac ccattcgact
     7321 tactgcccat ttaactggaa ttggattagc ttctacaaat aaatccgtgt gcagttttaa
     7381 aagtttatcg ttaattgctc gcgcatttgc caaatcactg gaaaaagcag cagcgcacat
     7441 ttcatgcatt aacttaggcg caacattagc tgttaccgaa atcacaccat gagcacctaa
     7501 aagcaatagc gcaagtgagg ttgcatcgtc accactataa accgaaaagt tttctggtat
     7561 ctgagacaat agatcacatc cacgccctat atctcctgtt gcatccttga ttcctataat
     7621 attagggacc gatgcgagct ttactaccgt ttgattggaa atatcagcaa cagttctacc
     7681 cggaacatta taaaggatca ttggaatatc aacggcttcg gcgattgtct taaagtgttg
     7741 atacattcct tcctgagtcg gtttgttata ataagggaca accgataaac aagctgccgc
     7801 gcccgcattt ttggcaaatt gtgttaattc gatagcttcc ctcgtcgagt ttgcgccagt
     7861 tccagcaata ataggaatgc gcccagcagc atgcgacact gctgtcttga tgagaagatg
     7921 atgctcttca aaatcaactg tgggcgattc cccagtggtt ccaactacga caataccatc
     7981 agatttctct gcgatatgga aatcgattaa ctcacaaaat ttctctagat cgagcgcacc
     8041 atcatcatgc attggcgtca cgattgcaac cagactacct ttaaacatag atctttatcc
     8101 actcaaaagg agtcatttta cctgatgcaa agctttatac tatattcttt agcttgattt
     8161 ggtgaaggtt attgtttcat cctcacaatc aacgtgaata gtatcttttg ccgcgaaatg
     8221 accttctaat agttcttttg ctaatggatt ttcaatttgg gtttgtatgg cgcgttttaa
     8281 aggacgggcc ccaaaaaccg ggtcaaatcc agcctctgca attttagcta atgctgcctc
     8341 tgatatttcg agatgaatct ccatttttgc tagacgattt tccaagttac gtaattgaat
     8401 ttttgcaatc gattgaatgt gttttgcatc cagcgcatga aatacgatga cttcatcgat
     8461 acgattgata aattctggac ggaaataggt tttaacttca cccattactg ctaatttgat
     8521 gatttgataa tcatctccgc tcatttgctg gatcatttgc gaacccagat ttgaagtcat
     8581 aacgatgact gtattcttaa aatcaaccgt tctgccttgt ccatcagtca tgcgtccatc
     8641 atctaacact tgtagcaata cgttaaatac gtcagggtgc gctttttcta cttcatcaag
     8701 tagaataacc gaatatggtt ttctacgaac catttcagtg agatagccgc cttcttcgta
     8761 gcccacataa ccgggcggcg cgccaatcaa acgagcaacg gaatgttttt ccataaactc
     8821 agacatgtcg attcgtatca ggtgatcttc agaatcaaat aaaaatgaag caagtgcttt
     8881 acataattcg gttttcccaa cacccgttgg gcctaaaaat aagaaagaac catacgggcg
     8941 attagggtct gataatcctg aacgcgaacg acgaatcgca tccgatatga ggcgagtagc
     9001 ctcatcctgt ccaactacac gttcatggag tttttgctcc ataagcagta atttttcacg
     9061 ttcaccttgc atcatttttg caactggaat accggtcgcg cgtgacacga cttcggcgat
     9121 ctcttctgca ccgacttgtg ttcgaaataa tttgggttta ttgagttccc tttcaggcag
     9181 ggtcgcttca gcgtgttgga cttgctcagc aagctgcgct tctagctttg gaatccgtcc
     9241 atactgtaac tcagatactt tttgccaatc acctttacgc gttgccactt caacttcttt
     9301 acgtagcttt tcgagctcct ccttgatttg ttgagaacct tgcgcacgca ttttttctga
     9361 cttgagtatt tcttcaagat ccgcatactc tcgttcaagt tttttgatct catcttctaa
     9421 taaggcaagg cgcttttgtg aagcttcatc cttttccttt ttcatcgctt cacgttcaat
     9481 cttgagttgt atgagacgac gatcaagttt atccatcact tcaggttttg aatccctttc
     9541 catacgaatt ctcgcagcgg cttcgtcgat aagatcgatc gctttatccg gcaaaaaacg
     9601 atctgtgatg tatcgatgag ataactcagc agcagcaaca atagctggat cggttatatc
     9661 aacgccatga tgcaactcgt agcgctcttg taagccacgt aaaatagcaa tcgttgcctc
     9721 aacggtgggc tcatcgacca aaactttttg gaaacgtcgc tccaatgccg cgtctttctc
     9781 gacatattta cgatattcat ccagggtcgt tgcgccaaca caatgcaatt cccccctagc
     9841 caaagccggt ttgagcatat taccggcatc tatcgcacct tctgcctttc ctgcaccaac
     9901 catggtatgc aactcatcga tgaacatgat tgttcgacct tcgtcttgtg atagctcttt
     9961 taagactgcc tttagtcgct cttcaaattc accacgatat tttgctcccg ctaataatgc
    10021 cgccatatcc aaggagagaa ctcgcttgtt tttaagcgta tcaggcactt ccccattgac
    10081 aatgcgttgc gccaaaccct caacaatcgc ggttttccca acccctggct caccgataag
    10141 tacagggtta tttttagtgc ggcgctgcaa aacttgaatc gttctgcgaa tttcatcatc
    10201 tcttccgatt acagggtcta atttaccggc acgcgcacgt tcggttagat ctaatgtata
    10261 tttttttaat gcttcacggc caccttcggc ctcagcactg gaaacttgtt caccgccacg
    10321 taccgattta attgcttgct ctaatgcact ccggcttgcg ccgtgttgtt tgagcaatag
    10381 ccctgtttca cctttatctt caagaagcgc tagcaaaaac atttcagaag caataaattg
    10441 atcaccatgc ttctgagctt ctttatccgt gaggttaagc aaattattca gctctcggga
    10501 aatattaatt tcgccgccac ctccctcaat ctttggcatg cgctcaaggt ttttagctaa
    10561 gccctcacgc aaaggattta cattgatacc cgcgcgttgt aataaagaaa ctgtgctgcc
    10621 atcttcttgc tgtaacaaag caagtaataa atgctgaggt tctatgtaag gatgatcttt
    10681 gcccaaagca atgctttggg catcgccgag agcttgctga aacttggttg taaatttatc
    10741 aaggcgcata cgaattccta gtagagatta aatatcctta caaatatggg ggatagttta
    10801 attttcaagg ctggtgatca aattgcttga ttcttttttt gttagttgct cgaatgattc
    10861 gctcaaaaat tgcaattatt tgatctgcta attggtattt ccataccttc aagcatgctc
    10921 agctcattca aagtaaagcc agctttctgt cgcgcctcat aatggaaagg gcccgtaatt
    10981 tgcccaccaa agtattgatt aacgagttca gaaaactttt tttctggatc tatttgccgt
    11041 tgattacaaa aaaaattata ccaacgagac ccaatagaga catgtccaat ttcatcacac
    11101 aggatgattt ctagtatctc aattgtcttt tcatccccga tttgtcgcaa gcgcttaatt
    11161 atacccggtg tcacatcgag ccctcgcgcc tcaagcacac gcggcacaag cgccatacgt
    11221 atcaatggat ccgaagcagt taacaaagcc atatcccata gaccattatg tgctggcaaa
    11281 tcaccataat catgatcaag ctcttttaat cgtttatgta gtaactggaa atgataggcc
    11341 tcttcagatg ccacggttac ccaatcatca tagaatgaat cgggtagccc tcgaaatcga
    11401 tgaatagcat cccatgcaag attaatcgca ttgaattcaa tatgcgctat tgcgtgaatc
    11461 agtgcaatca gacccaattt agttcccaat cgacgtcgtg gcacatcact cggcttcact
    11521 actacaagtt tagctggttg gcctggttga ttaattagtc gcatcggttc atgagattcc
    11581 agtgagagta atttattacg ccaagcctgg gttgcttgtt gggtcagcaa taacttctct
    11641 tcaatttcat ttgctatcaa gcaacttgca gcgagatcaa acagagatgt tttcattaga
    11701 tgaaatgggc aataatctgc aatctatgct attttttacc gatataacga tgtttttaaa
    11761 atgcaaatca aataagtaaa acttgtctag aaataaactt tgtctatcta agttcgttat
    11821 agaattccct gggtcacata taaactactt acaattgatt gataaggtag aaatgaatca
    11881 tccatgttaa tctctagcac cagaagacaa ataataatcc caatccattt aatacttagt
    11941 taatgcggta gttcacacca gaaaatattt atgcgatatg cctgctaaac aaaatattga
    12001 tcattctaaa tcaccactaa atgtaattat tagtgtggat gtagagcttt ggcctgacaa
    12061 ttgggatttg ggtcataccg agttcaatcg atgccttaac aaatttatcc atggaaaaac
    12121 aacctctggt aattatgggt taggattcca actagcgcag cttaaagaac ataatctaaa
    12181 atgtgttttt ttcgttgaat cgttattttc aggacattat ggcattgggc cattacagga
    12241 aattgttgca caaatccaag aagcggggca agaagttcaa ctacacctgc atacggaatg
    12301 gctaggtaga gggcgctttc ataatccact aaataaaaca ggctataatt tatttaattt
    12361 taatcaagca gagcaacaat tattaatctg ccaaggtaaa gaatttttag ctcaggcagg
    12421 agcagataat ctgcttgctt tccgtgctgg caattttggc agtaatttgc aaactctaga
    12481 agctgtacct caagctggtt ttattttcga tagcagcatt aacccgcgtt tttatataaa
    12541 aaatggatta gatactcctt tacatgtaga ggaatataaa gaaggaatat atgaatttcc
    12601 tttgacgatc tttaaagaat ggggaggacg tctgtcacaa tttcaatttg gtggttcatg
    12661 ttctttcaaa gaaatggcaa atctacttgg acaagcatgg attaacgaat ggcattcagt
    12721 tgtgatttta tcccacggat cagagttgct aaatcgtgca aagacacgac ctgataaaat
    12781 tgtggttgac cggtttgtac aaacatgccg gtttttagca aataatcgcg atttgttcaa
    12841 aacaatttgg ttctctgata tacaaccaga aaccatatat gctaaatcta aagagagttg
    12901 tgtattacag tccggtttta tcaatacagc tcatcgctat cttgagcaaa cgactcgtcg
    12961 cttgtatgga taataaagac tatgagtaat aacgctgtag aatcggtaga gtttaaattt
    13021 cgaattggtg aaataaccct ttggacatca catctgaagc tgaatgtatt tagaaatcac
    13081 ttcacggaat acgagcatcc ttatactgag gaaagtgatt attccgattc tattgacgag
    13141 aaacttgatg gcgttttatt tcgctcacgg ccaattacaa cgctattacc tagatttacg
    13201 ttaaaacaag acgtaatacg atatgctcct aagcaatatc atcgttactt tatagatcta
    13261 acagggtcaa aagaagaata tctagaaaaa ttctcagcta aatctcggtc aacattgaaa
    13321 cgcaaaataa aaaagttttc ggatcattgt ggcaatcaaa ttgattggcg agaatataag
    13381 acactagctg aaatggaaat tttttatcct ctagcaaggc aagtttccac taaaacttat
    13441 caagagcgta tgttagctgc gggtttacct gaagacgatt tatttattca gcgaatgtat
    13501 gcgcttgctc aaaaagactc tgttcgcgct tacttgttat tctgccaaga caagcctgtt
    13561 gcttatttat attgcccaat ttctaatgga atactatttt atcagtacct tggctatatc
    13621 ccagagatgg cacaatggtc acctggcaca gtactacagt gggttgttat agaaaattta
    13681 tttcttgaaa gtcattttgc tatgtttgac tttacagaag gtcctggaaa gggttctggt
    13741 ggacataaag agtttttttc tactaatagc acactttgtg ctgatatctt ttttctgaga
    13801 aaaaccaata ttaatttatt ccttgtttta ctacacacga aaatagattc attttccgca
    13861 aaaattggta ttttccttga tcgatttgga ttaaaaacaa aaataaagaa ttttcttaga
    13921 aaattatatc catccgcctc tcactaaatt agctagtacc gttttctagt ttatttgtca
    13981 gttctgataa agccctcgat actttatcag ataacacaga atcgaactgc gcgcgacgtg
    14041 cggtatctaa gtctaaaaat aatgcctttt aatctatcat gctctatcta ttattgatta
    14101 ttttagtcat tgctttaatt attggtcctg cttactgggt caaacatgtc atggagaaat
    14161 acagtcatcc tgataaccgc tacgcgcata caggggctga atttgctagg caattactcg
    14221 atcaagaatc tttgtcgcat gttaaagttg agctaactga gtcaggtgat cattatgatc
    14281 cactaaacaa agttgtccgt ttgacctctg ataaatttaa taatgcttca ttgacagcta
    14341 ttacagttgc agcccatgag gttggacatg caatccagga ccgagatgat tatttggctc
    14401 taaagttacg taccaaactt gtagtgcttg tcgctcctgc acaaaaactt ggcgctgcta
    14461 tcctgatgct agcgccagta tccgttatat tcattcggtc tccattggtc ggtggattaa
    14521 tgctagcagg tggttttttg agtttgggtt cagccgcatt agtacatata attactttac
    14581 ccatggaatt gaacgctagt ttctcgcgag cactaccact gctgaaaaag agtaaatatc
    14641 ttaaagaagg tgatgagtta catgctcata agctattaac tgcagcagct tggacttatg
    14701 tatctgcatc gctcatgact ttgctcaata tggcacgctg gtgggcaatt ttacgtcggt
    14761 aatattgtgc ttattttgga aaagcataat aaacttgaca cgctccctcc ctctactata
    14821 acttcgcaca ctgctcaagg tgctcattac gcttttgctt aatgagtgaa acgggaagct
    14881 ggtacaggcc agcgctgccc ccgcaacggt agatgagtca acaatctgca aatgccactg
    14941 taaatactac gggaaggcgt agattgggaa aatatttccg ctcataagcc cggagaccgg
    15001 cctcgagtga atgtcaaggt attgcggagg gcaatcgagc aaatgataca tatttcttaa
    15061 tggaatagcg catcatttat ttttttctcc tttgtaatat cacatccagt caatattttt
    15121 gcgcgggtgt gcgcgaagga aggaaaatga gatcgtgtct tttatttggt tttttcgtag
    15181 cgctatgttc tacaggctac gtatctgcta acaatgttca accgcctgtc attgttactg
    15241 ctacacgaac cgctcaaact gcagatgaga ctttggcttc tgcgacagta atcacacaag
    15301 ccgatattgc acggctacaa gcgcgatcgg ttgaagattt attgcgaggt gttcctggtt
    15361 taaatattgt taataatggt ggacctggca agcagacttc ctttttttta cgtggaaccg
    15421 aatcggacca tgtcttagtt ctcattgatg gggttcgtgt tggttcagtc acttcaggta
    15481 ccacctcttt tgaaaatatt ccaattcaac aaattgatcg catcgaaatc gtgcgcggcc
    15541 cgcgttcgag tttatatggt tctgaagcaa ttgggggggt tatccagatc ttcactcgta
    15601 aaggtggggg gcaaattaaa cctaatttta gctttggtgg cggtagttac aactcgatca
    15661 atggatcagc tggattagct gggggcggta atcagggttg gtttaacatc aatgtgagtg
    15721 gtattggtac gcgtggtttt aatgcgtgca caggttctga aacagcgggt tgcttcatga
    15781 ctgaagcctc acgagtagac gatcaagata aagatggtta ccggaacctg gcaagctcgc
    15841 tacgtgcggg atatcgcttt aatcaagcat tcgaagttga tgcacatttt ctgcatagtg
    15901 ccggagatac tcaattcgat ggtggttatg caaatcgttc gaaactgaag caacaagtac
    15961 tcggtgggtc tattcgatat tcaccatttt cattttggca attaaatttg actgcaggtc
    16021 gcagtttcga gaaattagat acttataaaa tcgaacgatc tgagcaaatc agaagaagta
    16081 catttttaag ccaatttgac agcatacgtg actcggtttt cttgcagaac aatttttcca
    16141 ttggcaacca tcagctatta actgcaggta ttgattattt taatgaccag gttaaaagta
    16201 cggaaagtta tctggaaact tctcgtagga attgggggtt atttgcacag catcaagctg
    16261 ccatcgcagc acataatcta caattatcga tgcgttggga tcataatgaa cagtttggaa
    16321 aatgggtcac gggtggaatc ggttggggtt atgcaattac tcaaaatttg cgtctaaccg
    16381 caaattttgg aaacgcattc aaagccccta attttaatga actctatttc ccgttctcag
    16441 gaaatccaaa tttaagacct gaatcatcac gcagttttga aatgggtatt accagcacaa
    16501 caaaacttgg aaagtggtca gttaatgcct atgaaacaca tattaacaat ttgattacgt
    16561 ttgatatcca aacgttttcg tcgatcaatg ttgaccgtgc tcgaattcaa ggacttgaaa
    16621 gcgttttcaa tactcaaata ctcaattgga caatcaatgc aaactttaca cttttgaatc
    16681 ctgagaatgc atcgtcaccc gatgataaga ttttgccgcg ccgggccaaa catattttta
    16741 gaattgatgc agaccgtcaa ttcaatcact acacgcttgg tggaaccttt ctggcagaag
    16801 gtaagcgttt cgatgatact gagaatacgc gattactcaa tggatttgtc agagtagatt
    16861 tgcgcgctga gtatgcattc gctcaaaatt ggcgattgca agctcgcctt gaaaatttat
    16921 tcaatgaaca ttacgaaaca gccgcatttt ttaaccaacc tggtcgaaat gttttcttca
    16981 cactccgcta tcaaacgtaa aatgacatca aattaacgat tcagtttatg aaaaatttgc
    17041 ttttcgattg caatgaattc aaagttgccc acactggaaa cgtaggtttt gctaatttag
    17101 ctcagcgacc tacaaacatt aaacctattt attcctccaa agagcactat cagatgttgc
    17161 ttttggagac acagcaagaa attggtcagt tacaggagat gctttatgcg tataaccgct
    17221 atgcaatact acttattttt caaggtatgg attcagctgg aaaaggcggt gttatcaagc
    17281 atgtaatgtc tggggtcaat cctcaaggct gccaggtttt tagtttccag caaccaagct
    17341 tggaagagct tgatcatgat tttttatggc gtagcaacaa gtgcttacct gaacggggac
    17401 gaattggtat ttttgatcgc tcttactacg aggaagtcat agtcgtccgt gtaatgcctg
    17461 atttgttgaa acagcaacgt ttgcccttgg aatgtgctga tcccaagaaa atatgggtag
    17521 aacgcttcca agatattatt aacctagagc actatttaca tcgcaatggg attcagatca
    17581 ttaaattttt cttgcatctg tcaaaagaag agcaacggca gcgcttaatt gcccgcatcg
    17641 atgaacccag caaaaattgg aaaataggac gagctgatct tgaagcccat aaacattggg
    17701 aggcgtatca attagcttac gatgagtgca tagatgcaac cagtacgcat catgcgcctt
    17761 ggtacataat tccggctgat gataagcgaa atgctcgttt actggtatcc catatcattg
    17821 ttgagacctt gcaagcctta aagatagctt atcctatcct cagcaaagat cagcttaaag
    17881 aattagcaac catacggcaa ttattagaaa atgagaagcc acctaattaa caaagtgggg
    17941 acgctgtggt gaaccaagcg gggaagatag taataaaccc ttttgaaacc aatgctgaat
    18001 aacatactca tagaactgtt gattacgaat tggtgacaaa gtcgcgtaga gcttttttcc
    18061 tgagctggtc agtaatacca cccccaaatc gagctgattt cccgcatctt gcgcgaatcc
    18121 tatcttagtc aatcttccat gataaaaaaa acgtgtatgt ttaccaagct tctttttgac
    18181 atacccttct ggcgcataat caaccagccc aattgccacc aatctttcga tcatcggtaa
    18241 ggttattttc tgattgacat aaacgtgatt agacaagtcg aagatcagtg gaatcggttc
    18301 tccttgaatc caaacaaact gacataggtg tttaaaacaa ttaatatcat cttgctcaaa
    18361 tatgaaatcc aaggatttac tctctttttg cctgatacca aactgatttc ctgcttgatc
    18421 agaaaaaatt tgctttatcc aagagagtaa attcattcct ttgtcattga ttgattatgt
    18481 tagtaatcga accattattt atcacgtttg gcgcgatgtg ttatttccct ttttacttcc
    18541 tcaagaaaat ctatattatt tttacgtcgt ttttcgctct gataccaggt aaaacaagtc
    18601 atcacgccca agatgattaa tacgacgctc aacaaagtaa ttccatcaaa aaagataaaa
    18661 acggctccgg ttgacactgc aaaagcacct agaagtgcag taacaatcca catcactttt
    18721 ccagattttt tttgtttttc acaccactct tcaagtttat tccgggttga cgcaagttct
    18781 tcatcggtcc acttttttat ttccattctc taactcctat ttcgatcttg agcgtaattt
    18841 taacgaatat cctcatctaa ggatatgttc tcaccgaaaa gtaaacaaaa atttgagcaa
    18901 gtttagaaaa caaccttatc tgttgatgtt tttgtgttag aaagctaaag cgattaagca
    18961 aaaattattc tacttaattc aaaaacttat aaaaattggt cgatagtatg catggaatga
    19021 ctaacttgat accgctattt atctctgcag atatgcgtag aatctttatg ataaacatta
    19081 atttcagtga ctttttagta tagacagata tgaataataa gaaaatcgaa aaacgagtca
    19141 ttcagttata cgaaaaactt gaattatcaa atgagataaa attacctgag aaagatgcgg
    19201 cacaattgat taggcatggt gcccatatta ttttccagct cgatacgcat aaccatttta
    19261 tttacctgaa tcctgcttgg gaaaccacta ctggtcttcc tgtagcaaaa actgtcaagc
    19321 tctccttact taattttgtt cacgaacaag atcatgatta tttaagaaaa aaactacaac
    19381 taatacgaac aagcgcgagc aagcatagtt ttcataccga atgccgcctt aaagtaaata
    19441 atactcagat cattcacgtt gaaatgcaat taatttattc cccagaaaat aacaaagcgc
    19501 agcacgtgat ggggataatg gttgatattt caaaacacaa gatttttcta gaaa
//
LOCUS       DAOQLJ010000026        19180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1000_length_19180_cov_6.398850, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000026 DAOQLJ010000000
VERSION     DAOQLJ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 19180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19180
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1000_length_19180_cov_6.398850"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..944
                     /locus_tag="PK522_03370"
     CDS             24..944
                     /locus_tag="PK522_03370"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNC40806.1"
                     /translation="MSMADRDGVIWYDGKLVPWRNATTHVLTHTLHYGMGVFEGLRAY
                     ETDQGPAIFRLPEHTERLFHSAHIFKMPIPFDPATLMQAQKDVVRENNLKSGYIRPII
                     FYGPEAMGLSAKNLSVHVAIAAWSWGTYLGPDSLLKGIRVKTSSFTRHHVNINMCRAK
                     SVSTYTNSILAHQEVALDGYQEALLLDVDGYVAEGSGENIFVIKNNKLYTPDLTACLE
                     GITRASVIELAGEIGLPIIEKRITRDEVYCADEAFFTGTAAEVTPIRELDNRTIGNGS
                     RGPITERLQTLFFECVSGKSKKHTDWLHYV"
     gene            969..1154
                     /locus_tag="PK522_03375"
     CDS             969..1154
                     /locus_tag="PK522_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HNC40807.1"
                     /translation="MNNRQIEVTVDDLPLHCPMPSMLSWNGHPRVYLPIEATREALCP
                     YCGTHYVLKGEVKTNHH"
     gene            1267..2643
                     /locus_tag="PK522_03380"
     CDS             1267..2643
                     /locus_tag="PK522_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNC40808.1"
                     /translation="MNDIPKEIFKAYDIRGIVNTVLTPDMVERIGHAIGSEAVKRKLS
                     SIAIGRDGRLSSPSLAQALAKGLQKSGLHVIDVGMVPTPVLYYAAHELCGYTGVMITG
                     SHNPPEYNGLKMVLGGETLAAETIQSLRKRLEENDLTHGEGSYKTHEITSEYLQRITS
                     DVKLSRPMKIVVDCGNGVTGAMAPALYRQMGCEVIELFCEIDGNFPNHHPDPSVPENL
                     QNVIETLAATDAEIGFAFDGDGDRLGLVTKDGSIIFPDRQLMLFAADVLTRNPGAKII
                     FDVKCTRNLALWIEQHGGIPIMWKTGHSFIKGKLKETGALLAGEMSGHIFFKERWYGF
                     DDALYTGARLLEFLSKQSDMNAVLHALPDAINTPELQIKLKEGENYALVDLLQREARF
                     DNPERIITTDGLRVEYRDGFGLIRSSNTTPVLVLRFEADNSDALQRIKLDFRRVLKQF
                     KPDISLPF"
     gene            2710..4227
                     /locus_tag="PK522_03385"
     CDS             2710..4227
                     /locus_tag="PK522_03385"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNC40809.1"
                     /translation="MKKYFHFIFILALLLPISTIADDLPELGDVSQATITPRQERQLG
                     YQIMRQIRADPSYLDDPEITSYLNNLGRRLLSTSNETQPGQSFEFFALQDPTINAFAL
                     PGGFMGFNSGLIIAAQSESELAGVMAHEIAHVTQKHLARMIAGQQHMGLISTIAGLAI
                     AILASRSNPQAGSAAIIASQAAAIQSRLNFTRKHEKEADRIGFNILHRAGFDPHGMSA
                     FFERLQLAGRYRENGAPSYLRTHPLTYERIADIESRMRELPYRQIPDSIDFHLVRAKL
                     KAALGNSTDTVAQFDSRIHDKRYSNELAERYGLIYALLRDKRFKRAEKELESLYQKMQ
                     SDPTIKNLRNHPLGDLILIESDYLQSGAMIDTLAARVKIANKQIPDAFKLYQAALKTY
                     PQHRALIYDYAAALLSHQGPQAALNFINKQSQVIHDDIHLYRLEAQCHSALGNHMLKH
                     RAQAEIYIQEGKLHAAVEQLEIALRYSNGNFYQLSSVEARLRQLKEFIASFEEES"
     gene            4385..5479
                     /locus_tag="PK522_03390"
     CDS             4385..5479
                     /locus_tag="PK522_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC40810.1"
                     /translation="MIYGYTRNHQPSAKEIYKTRLVDRGDVIQVISANGTLTPLELVN
                     VGTQVSGTVAKIYVDFNDQVKKGQILAELDQALLRAQLLQSEANYQSAQATLNITQNK
                     ALRGRTLLQKGFISKEALDELEQQLVLAKAQVAISKATVERDRANLNYSVIRSPISGV
                     VVARDINVGQTVAANFQTPILFQIAKDLYQMQINISVAEADIGQIQTTQEITFNVDAF
                     QEREFTASVKQIRLNPTIQENVVTYNVVAAVENRDGILLPGMTANVRFVINHKRDVLR
                     ISNAALRYKPNRDEAQISGVNQAPQGTQLYRLKNQVQQPVQVTTGVTDGHMTEIVSGD
                     LNVGDEIIVSDNKSSKSKESSASNFRFRMF"
     gene            5479..6216
                     /locus_tag="PK522_03395"
     CDS             5479..6216
                     /locus_tag="PK522_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC40811.1"
                     /translation="MSVVNQPAPNPILIQIDHLCKSYELTDRSGELISQPVLFDISLT
                     INQGEFIAIMGHSGSGKSTLMNILGCLDTPTSGNYWLSGRNVATMNSNELAHVRNQNI
                     GFVFQSFNLLKRMTALENIAIPLLYAGIGRKESLKRSQAMLDLIGLGKYRDHLPNQLS
                     GGQQQRVAISRALINQPQLLLADEPTGNLDTQTSHDIMTLFTQLNRNQGITIVLITHE
                     EDIAAHAQRLIRLKDGRIVQNEIKLSN"
     gene            6218..7441
                     /locus_tag="PK522_03400"
     CDS             6218..7441
                     /locus_tag="PK522_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC40812.1"
                     /translation="MNLLVIIGEALRALKLNRLRTGLTMLGMIIGVAAVVLMLSIGQG
                     AQININNTIASMGSHLLIVVPGATSSGGLRFGSGSVRTLTISDAQAVAELPSIEATAP
                     VISGIAQLSYSANNWSTSITGVTPDYFSVSNWELADGSIFADADLRSAIRVAVLGSVT
                     AENLFGNEDPIGKTFRISNKPFLVVGILAVKGQSLSGRDQDDNVFVPITTAQRQILGN
                     QFPGSINHMLVQAKSSDTLDEAEAEITQLLRQRHRIADKMENDFTVRNLTALATVAST
                     TAKVMAWMLGSIASISLLVGGIGIMNIMLVSVTERTREIGIRMAIGASYRIILMQFLL
                     EALVICILGGLIGVALGIGGAWMVSQSTDMPIVVTFNMIGLAFIFVTAIGIFFGFYPA
                     RKAAALKPVDALRYE"
     gene            complement(7547..7684)
                     /locus_tag="PK522_03405"
     CDS             complement(7547..7684)
                     /locus_tag="PK522_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40813.1"
                     /translation="MRFALFAAVLAAFMLTACGDPKPGQYPPSIMMERDSMPDKGEDK
                     K"
     gene            7968..8738
                     /locus_tag="PK522_03410"
     CDS             7968..8738
                     /locus_tag="PK522_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNC40814.1"
                     /translation="MIISSVLIAFRNLFNLKIQWILIWPLIVSCLIWILLSHFLWAHV
                     SGGIVEFISTTQIKDWLEESYLVKIANSLEGLVNILLFIALVIITTLMITALFVMPSL
                     INFVAKQYYPHLERKRGGSVMGNVKHVLSALLVFLLLWSIALPLWFFGIGIVISFIAA
                     AYFNQRLFCYDALSEHASPEELTQLLASNKPSLWSLGLLTGFLQFVPFLNLFAPTWTA
                     LAFVHYELGRLDKLRASTKQENPIANLSLNHKQSVDEE"
     gene            complement(8746..9297)
                     /locus_tag="PK522_03415"
     CDS             complement(8746..9297)
                     /locus_tag="PK522_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC40815.1"
                     /translation="MNVLNSTEIEQPSLLIVDDDSVFCEVLTKAMVKRGFEVICAHTI
                     ASAMEKIESLVPEYAIIDLKLADESGLTLVEKIRKLDPGTRIIMLTGYASIATAIEAI
                     KLGATHYLAKPVDVNEILAAFERTSGEANVPISPNPLSVDRLEWEYIHRILAENNNNI
                     SVTARILNMHRRTLQRKLAKKPV"
     gene            complement(9325..10632)
                     /locus_tag="PK522_03420"
     CDS             complement(9325..10632)
                     /locus_tag="PK522_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC40816.1"
                     /translation="MKSNARASHMFNRLLLSPLNKNLHRLFLLRSIAIILQCPLFFLV
                     YSFIEQDIPWAELIAVVVGLAIINMMTWYRLHQNWPVTNLEFFLQLLVDVLALTALLY
                     FSGGSTNPFISFYLLPLIIAATILPWRYTWLIAAISIACYTILLFEYVPLPHDHSDDH
                     SAHLMEFNLHVSGMWFTFVLSTVLIAWFIVRMSHSIRQRDKELAEAREQALCNEQIIA
                     LGTLAAGAAHELGTPLSTMAVIAGELQQEFPENGEYQKNIYTLRDQITHCKHILTQLL
                     ANAGQARTENASTQSVDVFLKQVLDKWTLIRPSVKFQYSCDGQQPVPHIMNTQLLSQS
                     ILNLLNNAADASVNQVKIRSYWDQNSLNLEIHDDGEGLTPEVMQHAGEAFFTSKAPGQ
                     GFGIGLFLANANIERFGGSVHLFNHPEGGACIHLTLPLLPQSS"
     gene            complement(10771..12144)
                     /gene="mpl"
                     /locus_tag="PK522_03425"
     CDS             complement(10771..12144)
                     /gene="mpl"
                     /locus_tag="PK522_03425"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HNC40817.1"
                     /translation="MHIHILGICGTFMGGLAVLARESGHKVTGCDLNVYPPMSTQLRS
                     LGIELIEGYSPEQLKLNPDLYVIGNVVTRGNPLMEAILNQNLPYSSGPQWLAEHVLRK
                     RWVLAVAGTHGKTTTSSMLAWILSFAGLDPGFLIGGIPENFGVSARLTGESVSGTNRE
                     ASPFFVIEADEYDTAFFDKRSKFVHYHPRTLILNNLEYDHADIFPDLASIQKQFHHLI
                     RTVPSNGLIVANGRESSLQQVIAQGCWTPIEFFGVPTEWAVKLSSQGAIEVYFNEACQ
                     GTLDWDLLGEHNLMNALAAIAAARHAGVPSQMGLLALSQFKNVKRRMEVKGIVNNITV
                     YDDFAHHPTAIRTTLEGLRTKVGEARIIAVLEPRSNTMKMGVWKDSLAQSLNIADQIF
                     CYTDQVDWDVTSALQSIKHKAVTYSQMQELINAVALMAHAGDHILIMSNGGFGGIHEK
                     LLLMLRK"
     gene            12306..13247
                     /gene="gluQRS"
                     /locus_tag="PK522_03430"
     CDS             12306..13247
                     /gene="gluQRS"
                     /locus_tag="PK522_03430"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HNC40818.1"
                     /translation="MRQIQETTKYRGRFAPTPSGMLHFGSLVTAVGSYLDARSHQGEW
                     LVRIEDVDHPRVKAGISEKILSTLEQFGMEWDGPVIYQSKCMNRYQDALHFLGSLGLT
                     YPCSCTRKEIADSALLGLEGMVYPGHCRNGLPPDHNQRPQAVRIKTKPDRIEFQDRLQ
                     GSVYQSIANEIGDFVLRRADGIYTYQLAVVVDDGWQGITHIVRGADLLASTPRQIFLQ
                     RLLGYPTPSYLHLPVVTTAQGEKLSKQTLAHPIDTENPLLQLMAAIDFLGQHPPSELI
                     EGDLNSFWQWAINHWSVTRIAQVTYAPYNPSLNIVNE"
     gene            13328..14485
                     /locus_tag="PK522_03435"
     CDS             13328..14485
                     /locus_tag="PK522_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC40819.1"
                     /translation="MAVLPFYISNKKNLKIVIVGGGYAGIAALTTIARYQPSAEITLI
                     DPKIDHIKITHLHETFRYPLTDFLIPFTDIEQEFGCRHLPLELPFSESTLLQWERDKT
                     ISINNESIPFDYLLIASGALNNNADQDSGDVMGLNAFMKHPGSEILSQFLSKPGTEAP
                     ISVIGGGATGIQFLFEIEHFLRYHKYKNKLRLIDSGDQVLKQFPDRFARYVEAAMKNL
                     NIEYYPTTYFRGQQHSQIRLEDKKTSHQFELPSSLTFLFSGKSQGSVLKANAFGQVIV
                     DQQILQHIFVAGDCAYYQSIGSNALTAQSAVRKGKLAARNLLRHSGLLKILEPYLHRD
                     IGYVVSLGPADAIGWLALENNVVTGLAAYTIKEIVEAQYDLLLAGIDTYLL"
     gene            14510..14980
                     /locus_tag="PK522_03440"
     CDS             14510..14980
                     /locus_tag="PK522_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HNC40820.1"
                     /translation="MFNLFDDKPVAGKASTVIQSPDDKLFNFIGHELLTNYPRWSPEV
                     KELEKLTDSPLGLGTLCRQVRVDQGNRSESTFKIAVFEPNKQICFNGVSNPFRCDYSL
                     ESKNQESTQITFTFELLSLELHMRPFEKLIRIAVQDGAERTVRNIKRLIEFNTI"
     gene            15046..15498
                     /locus_tag="PK522_03445"
     CDS             15046..15498
                     /locus_tag="PK522_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNC40821.1"
                     /translation="MDFIVEKDPGLIPQVLSNILDSCVNGITLADPDQEDLPLVYANK
                     AFETITGYTQAETIGKNCRFLQGNDRDQQERHQLREAVLNKQSIEITLRNYRKNGEMF
                     YNHLVITPLFDSKGNVLYYLGVQFDVTQQKKAEEEIKRLTEQLAKLSK"
     gene            15617..15704
                     /locus_tag="PK522_03450"
     tRNA            15617..15704
                     /locus_tag="PK522_03450"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15651..15653,aa:Ser,seq:tga)
     gene            16085..16405
                     /locus_tag="PK522_03455"
                     /pseudo
     CDS             16085..16405
                     /locus_tag="PK522_03455"
                     /inference="COORDINATES: protein motif:HMM:NF023583.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            16460..17746
                     /locus_tag="PK522_03460"
     CDS             16460..17746
                     /locus_tag="PK522_03460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNC40822.1"
                     /translation="MGLVFEELIRKFAEISNETAGEHFTPREVIRLMVNLLFIEDDEV
                     LAKPGVVRTIYDPTAGTGGMLSVAGEYLEEHNPQARLTMFGQELNDESYAICKADMLI
                     KGQDVANIVAGNTLADDGHPHKKFDYMLSNPPFGVEWKKVEKEIRKEFESQGYNGRFG
                     PGLPRVSDGSLLFLLHLISKMRPAHEGGSRFGIVLNGSPLFTGGAGSGESEIRRYVLE
                     NDLVEAIIGLPTDMFYNTGISTYVWILSNHKPEHRKGLVQLIDASGFWQKMRKSLGSK
                     RKEFSEAHIAEITHLFGHFEEASKDGAPISRIFRNEAFGYRTITVERPLRDANGQIVL
                     GEKGKLKGKPQPDSSLRDTENVPLSEDVDVYFKWEVLPHAPDAWIDHEKTKIGYEIPF
                     NRHFYVFKPPRELAEIDADLKQCTDRILAMIKGLSA"
     gene            17743..19080
                     /locus_tag="PK522_03465"
     CDS             17743..19080
                     /locus_tag="PK522_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNC40823.1"
                     /translation="MSFPRYEHYKDSAVEWLGEVPEHWEIKHLKHSCQIYPSNVDKKS
                     YENESPILLCNYTDVYYNESITNQLQFMSATASADQINKFSLKAGDTIITKDSETADD
                     IAIAAYVPEDLPGVICGYHLALVRPFPEACGAFIKRLFDSHYAKASFNVLANGLTRVG
                     LGQYALNNLEIPFPPYIEQSIIATFLDRETAKIDALVAEQQGLIELLKEKRQAVISHT
                     VTKGLNPDTPMKDSGVEWLGEIPEHWELKATKRLFRMVVEPAPDDNDHELLSIYTAIG
                     VRPRKELEEKGNKATTTDGYWMVRKGDLIVNKLLAWMGAIGISDYDGVTSPAYDILRQ
                     RIPLEPRYYDYLFRCGICFSEFRRYSRGIMDMRLRLYFEELGQLHMPFPPELEQQQIV
                     SFLQEELVIFDELTAEAQRAITLLQERRTALISAAVTGKIDVRGLVSDKRAAE"
BASE COUNT         5617 a         4226 c         4092 g         5245 t
ORIGIN      
        1 gaatttttat cgaggatttt gtaatgtcaa tggctgatcg cgatggtgta atttggtatg
       61 acggaaagtt ggttccttgg cgaaatgcta caacccatgt gctaacccat acgttgcact
      121 atggtatggg tgtatttgaa gggttgcgtg cttacgaaac cgatcagggc cctgcgattt
      181 tccgcttacc ggagcatacg gagcgcttat ttcattcggc acatatcttc aagatgccca
      241 ttccattcga tccagcaaca ctcatgcaag cgcaaaagga tgttgtacgc gaaaataatc
      301 ttaaatcggg ctatattcga cctattattt tctacggccc agaagccatg ggactctctg
      361 ccaaaaatct atcggtacat gtcgcaattg cagcttggtc atggggcact taccttggac
      421 cggatagtct tttaaaagga attcgcgtca aaacttcatc gtttacccgg catcatgtca
      481 atattaatat gtgtcgagcc aaatcagttt cgacttatac caattcgatt cttgctcacc
      541 aagaggtagc actcgatggt tatcaagaag cgcttttact ggatgtcgat ggttatgtgg
      601 ctgaagggtc cggtgaaaac atctttgtta ttaaaaataa taagctgtat acccccgatc
      661 tgacggcctg tctcgaagga attacacgtg cttcggttat cgaattggcg ggggaaattg
      721 gccttcctat catcgagaag cgaattacca gagacgaagt ttactgtgcc gatgaagcct
      781 tctttacagg aacggctgca gaagtaacac cgatcaggga gctagataat cgaacgatcg
      841 gaaatggctc gagaggtccc ataactgagc ggttacaaac tttatttttc gaatgtgtca
      901 gtggcaaatc aaaaaaacat actgattggc tacattatgt ctgattttat taaggggaat
      961 ctattacgat gaataaccga caaattgaag taaccgtaga tgacttgccg ctgcactgcc
     1021 caatgccctc catgttgtcc tggaatggcc atccccgtgt ctatttaccc attgaagcga
     1081 ctcgcgaggc cttatgtcct tactgcggca ctcactatgt tttaaaagga gaggttaaaa
     1141 caaatcatca ctgaaataat ggtattgggt gtaaagcaca ttcaatacag gaaagaaaat
     1201 cattaatcct atcttttctg ctatgcatac cactcacatc taacttaatc gataaagagc
     1261 cctgctatga atgacatccc taaagaaata ttcaaagctt atgatattag aggcatcgtc
     1321 aataccgttt tgacccccga catggtagag cgaattggcc atgccatagg gtcagaggcg
     1381 gttaaacgta aactctcctc aattgccatt ggtcgtgatg gaagactatc cagcccttcc
     1441 cttgcgcaag cgctggccaa agggctacaa aaaagtgggc tacatgtcat cgatgtcggc
     1501 atggttccaa cccctgtgct ctattatgca gcgcatgagt tatgcggtta cacgggggta
     1561 atgatcacgg gcagccataa tcctcctgag tataacggtc tcaaaatggt attaggtggc
     1621 gaaactttag cagcagaaac cattcaatcc ttacgcaaac gcttagagga aaatgatcta
     1681 actcatggtg aaggtagcta taaaacccat gaaatcacat cagaatacct ccaaagaatt
     1741 accagtgatg tgaaattatc gcgcccgatg aaaatcgtcg ttgattgtgg taatggtgta
     1801 accggtgcga tggcgcccgc gctttatcga caaatgggtt gtgaagtcat cgaattgttt
     1861 tgtgaaattg atggtaattt tccgaatcat catcctgacc catccgttcc tgaaaatttg
     1921 cagaatgtca ttgaaaccct tgcagcaact gatgcagaaa ttgggtttgc atttgatggc
     1981 gatggggatc gattaggtct cgttaccaaa gatggcagta ttatttttcc tgatcgtcag
     2041 ctcatgttat tcgcggctga tgtattaacc cgcaatccag gggcgaaaat tatttttgat
     2101 gttaaatgta cccgcaacct ggcgctatgg atcgaacaac atggtggtat tcctattatg
     2161 tggaaaaccg ggcattcctt tattaaaggc aaactaaaag aaacaggggc actattagca
     2221 ggtgaaatga gtgggcatat ctttttcaaa gagcgatggt atggatttga tgacgcctta
     2281 tatacgggtg cacgtttatt agaatttttg agtaaacaat cggatatgaa cgcagtgctt
     2341 catgcattac ccgacgcgat caatacaccc gaactacaaa ttaagctcaa agaaggtgaa
     2401 aactatgcct tagtcgattt actgcaacga gaggctcgtt ttgataatcc tgagcgcatc
     2461 atcacaaccg atgggttgcg tgttgagtat cgtgatggat ttggtttgat tcgttcttcc
     2521 aacaccacac ctgttttagt attgcgtttt gaggcagata attctgatgc gctacaacgg
     2581 attaaactcg attttcgtcg agtattaaag caatttaaac cggacatcag tttgcccttt
     2641 taaaaacttg acccaaccag gcaatcgctg tcactggaac tcgaatcgaa agtcatcatc
     2701 caatctggca tgaaaaaata ttttcacttt atttttattt tggcgctgct attgccaatt
     2761 agcacgattg ctgatgattt gccggagctc ggtgatgtct cacaggcaac cattacacct
     2821 cggcaagaaa gacagcttgg ttatcaaatt atgcgccaaa tccgagctga tcctagttat
     2881 ctcgatgacc ctgaaattac cagttactta aacaacctgg gaagaagatt gctatcgact
     2941 tccaatgaaa cacaacccgg ccaatcattt gaattctttg ctttacaaga tcctacgatt
     3001 aatgcgtttg cattacctgg gggattcatg ggttttaata gcgggttaat cattgccgca
     3061 caaagcgaat cagaactggc cggtgttatg gcgcatgaga ttgcccatgt cactcaaaaa
     3121 cacttagcaa gaatgatagc ggggcaacaa cacatggggt tgatatcaac cattgccggt
     3181 ttagctatag caattctagc atctcgctct aatccacaag ccgggtcagc cgctatcata
     3241 gcaagccagg ccgccgccat tcaatcacgc cttaatttta cgcgcaaaca cgaaaaggaa
     3301 gccgatcgca ttggcttcaa tattctccat agagcgggat tcgatcccca tggaatgtca
     3361 gctttttttg aaagattaca attggcgggt cgctaccgcg aaaatggcgc accttcctat
     3421 ttaagaactc accccctgac ttacgagcgc atcgcggata ttgaaagcag gatgcgtgag
     3481 ttaccctatc gtcaaatacc ggatagcatc gactttcatc tagtacgcgc taaattaaag
     3541 gctgccttgg gaaactcgac tgacaccgtt gcgcagtttg attcgcgcat tcacgacaag
     3601 cgctactcaa atgagttagc agaacgttat ggcttaattt atgcgctcct acgagacaag
     3661 cgctttaagc gtgctgaaaa agagctagaa agcctttatc aaaagatgca atctgatccc
     3721 acgataaaaa acctccgcaa tcaccctctt ggagatctta tcttaatcga aagtgattat
     3781 ttacaatcag gggcaatgat tgatacgctt gcagcacgtg taaaaatcgc taataaacaa
     3841 ataccggatg cctttaagct ttatcaagca gcattgaaaa cctatccaca gcaccgggca
     3901 ttaatctatg actacgcagc cgccttattg agtcaccaag gcccacaagc cgccctcaat
     3961 tttatcaata aacaatcaca agttattcac gatgatattc atctttaccg cctagaagcg
     4021 cagtgccatt ctgcgcttgg caaccatatg ctcaagcatc gtgctcaggc agaaatctat
     4081 attcaagaag gcaagctaca tgccgccgtt gaacaacttg agattgcgct tcgctatagc
     4141 aatggtaatt tttatcagtt atcgagtgtt gaagcgcgct taaggcagct taaagaattc
     4201 attgcaagtt ttgaggaaga gagttgatct attgccttga aattgaccca atcatgctaa
     4261 caaggttaca cacacttagt tagcaaatcc ctaatcagca ccaacacttg ttttatctct
     4321 ttccatgata cgtcgcaatt tcagaacttt gtccttcata ttatttctcc taatattagg
     4381 cgctgtgatc tatggatata ctcgaaacca tcaaccgtca gcaaaagaaa tctacaaaac
     4441 acgccttgtc gatcgaggag atgtcattca agtcatttct gcaaacggca cattaacacc
     4501 gcttgagctg gtcaatgtag gaacacaagt atccggcacg gttgcaaaaa tatatgtaga
     4561 ttttaatgat caggtcaaaa aaggacaaat attagctgaa ctggatcaag ctttactccg
     4621 cgcacaattg cttcaatcag aagccaatta ccaaagtgca caagccacct tgaacatcac
     4681 tcaaaataag gcactgagag gtcgaactct gttacaaaag gggttcattt ctaaggaagc
     4741 tttagatgaa cttgagcaac agcttgtatt ggccaaagct caagttgcaa tcagcaaagc
     4801 aacggttgaa cgtgatcgag ccaatcttaa ctatagcgtg attcgctccc ctatttctgg
     4861 tgttgtcgtt gctcgagata tcaatgtagg ccaaaccgtt gctgcaaatt tccaaacacc
     4921 gatcttattt caaattgcaa aagatcttta ccaaatgcaa atcaatatca gtgtcgctga
     4981 agcggatatt ggtcaaattc aaacgacaca agaaatcaca ttcaatgtcg atgcgtttca
     5041 agaacgcgaa tttaccgcat ccgtcaaaca gatacgctta aaccccacta tccaagaaaa
     5101 tgtcgtaacg tataacgttg tcgcagcagt tgagaatcga gacggaattt tactccctgg
     5161 aatgactgct aatgttcgat ttgtcattaa tcacaaacga gatgtgctaa gaatctccaa
     5221 tgcagcgttg cgatataaac cgaatcgtga tgaagcacaa atcagtggtg ttaatcaggc
     5281 accgcaaggt acccagcttt atcgtttaaa aaatcaagtg cagcaaccag tccaagtcac
     5341 cacaggcgta acagatggtc acatgaccga gattgtttct ggtgatctga atgtgggtga
     5401 tgaaatcatt gtgagtgata acaaaagtag taaatcgaaa gaatcttctg ccagcaactt
     5461 tagattcaga atgttttaat gtctgtagtt aaccagcctg ctcccaaccc aatcttgatc
     5521 caaatcgatc atttgtgtaa atcctatgaa ttgaccgatc gttcgggaga gttgatcagc
     5581 caacctgtat tattcgatat cagcttaaca atcaatcaag gtgagtttat agccatcatg
     5641 gggcattctg gatctgggaa atcaacgctg atgaatatct taggttgcct tgatacgcca
     5701 accagtggca attattggct ttctggccgc aatgttgcca cgatgaatag taatgagtta
     5761 gcgcatgttc gtaatcaaaa tattgggttt gtgtttcaaa gctttaattt gttaaaacgc
     5821 atgacagcat tagagaatat cgctatcccc cttctttatg ctggcattgg acgcaaagaa
     5881 agtctaaaac gatcgcaagc aatgcttgat ttaataggat taggcaaata tagagatcat
     5941 cttccaaacc aactttctgg ggggcagcaa caacgcgttg caattagtcg tgcgctcatc
     6001 aaccaacctc agcttctatt agcggatgag cccacaggta atctcgatac tcaaactagt
     6061 cacgacatca tgactttatt tacacagctc aatcgcaatc aaggaattac tatcgtatta
     6121 attacccacg aagaagatat cgccgcgcac gcacaacgcc tcatccgatt aaaagatggt
     6181 cgtatcgtcc agaatgagat caaactgagt aactagcatg aatctactcg ttatcattgg
     6241 tgaagcgtta cgtgcattaa aactcaatcg attacgaacc gggttaacaa tgcttggcat
     6301 gattattggt gtcgctgcgg tcgtactgat gctatcgatt ggccaaggtg ctcaaattaa
     6361 tatcaataat acgattgctt ctatgggaag tcatttattg atcgttgttc caggcgctac
     6421 ttcttctggc ggtttacgat ttggtagtgg cagtgtgcgg acattgacta tcagcgatgc
     6481 acaagccgtt gctgaattac ccagcattga agcgacagcg cccgttatct caggaattgc
     6541 tcaactcagc tatagcgcta acaattggag cacctctatc actggcgtaa cacccgatta
     6601 cttttctgtc agcaattggg aattagcaga tggttcaatc tttgccgacg cagatttacg
     6661 ttctgcgata cgcgttgcag tcctaggtag cgttaccgct gaaaatttat ttggcaatga
     6721 agatcccatt ggtaaaacct ttcgaatttc caataagccc tttctcgtgg tgggaatact
     6781 cgccgtaaaa ggacaaagct tgagtgggcg tgatcaagac gataatgtct ttgttccaat
     6841 cacaacggcg caacgccaga ttctgggcaa ccaattccca ggatcaatta atcatatgct
     6901 tgttcaagca aaatcatccg atacattaga cgaagcagaa gcagaaatta ctcagttatt
     6961 aaggcagcgg catcggatcg cagataaaat ggagaatgat tttacggtac gcaacttaac
     7021 tgcattggca accgttgcaa gtactactgc taaggtaatg gcctggatgc tcggttcaat
     7081 tgcctcgatc tcgctattag tcggcggaat cggtattatg aatatcatgc tcgtttctgt
     7141 tacggaacga acccgagaaa ttggtattcg tatggcaatt ggcgcaagct atcgaatcat
     7201 tctaatgcaa tttcttctag aagcgttggt tatctgtata ttaggcggac taataggagt
     7261 tgctttaggg attggtggcg cttggatggt gagccaatcc actgatatgc ccatcgtggt
     7321 cacgtttaat atgatcggat tagcatttat attcgtaaca gcaatcggta tattttttgg
     7381 attctaccca gcgagaaaag cagctgcttt aaagccagtc gatgcactcc gttacgagta
     7441 gagcgttaac acaccgttca agtgccatta ttcaatttta aatccactac caataaaaaa
     7501 accgccccca gtgatgaagg gcggtttcga aattgagtca attttcttat tttttatctt
     7561 cacccttatc aggcattgag tcacgttcca tcataatgct gggaggatat tgccctggtt
     7621 ttgggtcacc acacgcagtt aacataaacg ctgctagtac agctgcaaaa agtgcaaatc
     7681 tcattttagt ctcctattaa acaaatatga tgaattaact tattttattt aacttcttta
     7741 actgctctta gaccgttcat atctaacagc cacctcttta atagcagaat tacacaaggc
     7801 tgtccagaaa tttatgtatc aagaaaatga atgagcagtt attacctcaa cgatacaaga
     7861 gcttactcta taggtaacaa aactaactat ataataacca ctgtcaattt tttattaaca
     7921 tttaggctcg ttcaatttta ttttgtaatg aagaggttca atgacacatg attatcagtt
     7981 cagttttaat tgcttttcgt aatcttttta atttgaagat tcaatggata cttatttggc
     8041 cattaatcgt ctcatgcttg atttggatct tgctgagtca ctttctttgg gcccatgtat
     8101 cgggtggtat cgtagagttc atttcaacga ctcaaattaa agattggctt gaagaatcat
     8161 acttagtaaa aatcgcaaac agcctggaag gtcttgttaa tatccttctt ttcattgcgc
     8221 tcgttatcat cactacgcta atgataaccg ctttgtttgt tatgccttcc ctcatcaatt
     8281 ttgttgctaa acaatactat ccccatcttg aacggaagcg gggcggtagc gtcatgggta
     8341 atgtaaagca tgtactatca gcgctattgg tatttttatt gctttggagc attgcattgc
     8401 cgttgtggtt tttcggcata gggattgtga tttcatttat tgcggccgcg tatttcaatc
     8461 aacgattatt ctgctacgac gcactatccg aacacgctag tccagaggaa ttaacccagt
     8521 tgttggcttc taacaaaccc tctctctgga gtcttgggtt gctaacagga tttctgcagt
     8581 ttgtcccctt tcttaattta tttgcgccca catggactgc gctcgctttc gttcattacg
     8641 aacttggacg gcttgataaa ttacgggcta gtacaaagca agaaaacccg atagcaaact
     8701 tatcacttaa ccataagcaa agtgtggatg aggagtaaca accgattaaa ccggtttttt
     8761 tgcaagtttg cgctggagtg ttctgcgatg catattcaag atccgagccg ttaccgaaat
     8821 attgttatta ttctctgcaa gaatgcggtg aatatattcc cattctaagc gatccacgga
     8881 caatggatta ggactaatgg gaacatttgc ttcacccgat gttcgctcaa atgcagctaa
     8941 tatctcgttc acatcgacgg gtttggcaag gtaatgagtc gcgccgagtt taatggcttc
     9001 gatggcggtt gcgatactcg catagccggt cagcatgatg attcgggtcc ctgggtctaa
     9061 ttttctgatt ttttcgacga gcgtcaagcc agactcatct gcgagtttta aatcgataat
     9121 cgcgtattca ggcaccaacg actcaatttt ttccattgca gatgcaatgg tatgggcgca
     9181 aatcacttca aatccccgct tgaccattgc ttttgtaagc acttcgcaaa aaacactatc
     9241 atcatcgaca atgagcaatg aaggttgctc tatttctgtg ctattcagta cattcattga
     9301 cagttcattg cataagcaat tgcgttagga agattgaggt agtaggggga gtgtaagatg
     9361 aatacacgcc cccccttctg gatggttaaa taaatgtacg ctgcctccaa agcgctctat
     9421 attggcattg gcgagaaaca atccaatacc aaaaccttgg ccaggcgctt tactggtaaa
     9481 gaaggcttcc cctgcatgtt gcatcacttc gggtgttagg ccttcaccat catcatgaat
     9541 ttctaggttc agcgaattct gatcccaata actccgaatt ttgacttgat tcacagatgc
     9601 atcagccgca ttgttcaata aattaagaat agactggctg agtaactggg tattcatgat
     9661 atgcggtaca ggttgttgac catcacaact atactgaaac tttacagacg gacgaatgag
     9721 cgtccactta tccagcactt gcttcaagaa cacatcgaca gactgagtgc tcgcattctc
     9781 agttctggct tggccggcat ttgccaatag ctgagtcaat atatgcttgc aatgcgtaat
     9841 ttgatcgcgc aatgtataga tatttttttg atattcacca ttctcaggaa attcctgttg
     9901 gagctcacct gcaataacag ccatcgtcga caaaggtgtt cccagttcat gagccgcgcc
     9961 ggctgcaagt gtcccaagtg caataatttg ttcgttacac agcgcttgct ctcgcgcttc
    10021 agcaagctct ttatctcgtt gacgaatgga atggctcatt ctaacgataa accatgcaat
    10081 taagacggta ctcaatacaa aggtaaacca catgcctgaa acgtgcagat taaactccat
    10141 gagatgtgca ctatgatcgt cagagtgatc atggggtaac ggtacatatt caaacaataa
    10201 aatggtataa catgcgatgg aaattgcggc gattaaccag gtataacgcc agggtaagat
    10261 cgttgcagcg ataatcagtg gaagcaagta aaacgagatg aaaggattgg ttgatccccc
    10321 gctgaaatac aagagcgcag taagcgccaa tacatcgacc agaagctgta aaaaaaattc
    10381 tagattggta actggccaat tctgatgaag tcgataccaa gtcatcatat tgataatggc
    10441 tagcccgaca acgacagcaa tcaattcagc ccagggaata tcttgttcga taaaagaata
    10501 aaccaagaag aacaaagggc attgcaatat gattgcaata cttcttaaaa gaaatagccg
    10561 atgtaaattt ttgttgagtg gggaaagaag gagtcgattg aacatgtgac ttgcgcgcgc
    10621 attacttttc atacaattta attttaatgc tacattagcg taaattaacc aactaaacca
    10681 tgcgacaaat tgtcgcacat caagtatctt agccgctcaa attcccctcg atcccctggc
    10741 aggaacgtat ccaccgacaa ttgaatcgat ttactttcgc agcattaaca gcagtttttc
    10801 atggatgccg ccaaaaccgc cattactcat aattaaaata tgatcacctg catgcgccat
    10861 gagggcaacc gcattgatca attcttgcat ctggctgtat gtaactgctt tatgtttgat
    10921 tgattgtagc gctgacgtta catcccaatc gacttgatcg gtatagcaaa atatttgatc
    10981 tgcgatattt aagctttgag ccaaactatc tttccatacg cccattttca tggtgtttga
    11041 gcgaggttcc aaaacagcaa taattcgagc ctcaccaact ttagtgcgta acccctctaa
    11101 cgttgttcga attgcagtag gatgatgagc aaaatcgtcg tatacggtaa tattgttgac
    11161 gatacctttc acttccatac gacgcttgac atttttaaac tgcgataaag caagcaaacc
    11221 catttgagag ggaacgccag catggcgcgc tgccgcaatt gcagccaagg cattcattag
    11281 attatgttcc cccaataaat cccaatctaa tgtaccttga caggcctcgt tgaaataaac
    11341 ctcaattgca ccctgtgaag ataacttaac tgcccactct gtcggtactc caaaaaactc
    11401 gattggtgtc cagcagcctt gagcaataac ttgctgcaga ctactctcac gcccattcgc
    11461 aacgataagc ccgttgctag gaacggtccg gattaaatga tgaaactgtt tctgaatcga
    11521 tgctaaatca ggaaaaatat cagcatgatc atattcaagg ttatttaata ttaatgttct
    11581 gggatgataa tgaacaaatt ttgaacgctt atcaaagaaa gctgtgtcat attcatccgc
    11641 ttcaataaca aaaaaaggcg aagcttcccg gttcgttcca gaaacagatt ctccggttag
    11701 tcgtgctgag acaccaaaat tttctggtat ccctccaatc aaaaaacccg gatccaatcc
    11761 agcgaaagaa agtatccaag ctaacatcga gctcgttgtt gtttttccat gggtaccggc
    11821 tacagctagc acccatctct ttctaagaac atgctcagcg agccactgag ggcctgaact
    11881 ataaggtaaa ttctgattaa gaattgcttc catgagggga ttaccccgag tcacaacgtt
    11941 accaattaca tacaagtcag gattgagttt gagctgttcc gggctatacc cttcaattaa
    12001 ctctataccc agagaacgga gctgagtact catcggtggg tagacattaa gatcacagcc
    12061 ggtaacctta tgacccgatt cgcgcgccag cacagccaat ccacccataa atgttccaca
    12121 gataccaaga atatgaatat gcacgatggc ctctacccaa aaaatagtct atcgaattga
    12181 atacaaagtt gttcactcat catcttaaat gcgcttaaat tatactgacg attgatagtc
    12241 gttctgatag tagttcgaaa atgggttaac ccactatttc aataacacgg tatccagcct
    12301 gaataatgcg tcagatacaa gaaactacaa agtatagagg acgttttgca cccacaccaa
    12361 gtggtatgtt gcattttggc tcgctcgtta ctgcagtggg tagttatctt gacgcacgat
    12421 cacaccaagg tgaatggtta gttcgtatcg aggatgtcga tcaccctcgg gtgaaagcag
    12481 gtatatcgga aaaaatttta tccacattgg aacaattcgg tatggagtgg gatggtccag
    12541 tcatttacca aagtaaatgc atgaatcgtt accaagatgc gctccatttt ttggggtcat
    12601 taggattgac ttacccctgc agttgcacgc gtaaagaaat tgcagattcc gccttattgg
    12661 gattagaggg tatggtttat ccggggcatt gtcgtaatgg cctccctcca gaccataatc
    12721 aacgtcccca agcggtgaga ataaaaacga agcctgatag aattgaattt caagatcgtt
    12781 tgcaagggag cgtttaccaa tcaatcgcaa atgaaattgg cgattttgtt ttaagaagag
    12841 ctgatggaat ctatacctat caattagcag tcgtagttga tgatggttgg caaggtatta
    12901 ctcatatcgt gcgaggtgct gatttgttgg catcaacccc tcgccagatt tttctacagc
    12961 gtcttctagg atatcctacc ccctcctatc ttcatttacc tgtagtaaca actgctcaag
    13021 gtgagaaatt aagcaagcaa acacttgcac acccaattga tacggagaat ccactcttgc
    13081 aactcatggc agcgatcgat ttcttgggcc agcatcctcc atcagaatta attgaaggcg
    13141 atttgaattc gttctggcaa tgggctatca atcattggtc agtaacacgc atcgctcaag
    13201 tcacgtatgc tccctataac ccgtcactaa atatagtcaa tgaatagact tctcatcgcc
    13261 atttcgtgct acgcttgcct aattagctca tgaataatga ttcaatctag gaattcgagg
    13321 tggatgtgtg gccgttttac cgttttatat ctcaaacaaa aaaaatctaa aaattgtcat
    13381 cgtaggcggt ggatatgctg ggatagctgc actaacaacc attgctcgtt atcaacccag
    13441 tgctgagata acgctgatcg atccaaaaat cgatcacatc aaaattactc atctccatga
    13501 aacctttcgc tatcctttga ctgatttttt gatacctttc actgatatcg aacaagaatt
    13561 cggctgtcgc catctaccct tagaattacc tttcagcgaa tcaacgctac tacagtggga
    13621 acgtgataaa actatttcga taaataatga atcaatccca tttgattatc tgctaatcgc
    13681 ttcaggtgcg ctgaataata atgctgatca agactcaggc gatgtaatgg gattgaacgc
    13741 ttttatgaag catccgggat ccgaaatact gagtcaattc ttatcgaaac caggtactga
    13801 agcacctata tccgttatag ggggaggcgc aaccggtatt caatttttat ttgagattga
    13861 acacttctta cgctatcaca aatataaaaa caaattgcgc ctgatcgatt ctggagatca
    13921 agtattaaaa cagttccccg atcggtttgc gcgctatgtt gaagccgcga tgaagaatct
    13981 aaatatcgaa tattacccaa cgacttattt tcgtgggcag cagcatagcc aaataagatt
    14041 agaggataaa aaaacaagcc accaatttga acttccttca agtttaactt tcttattttc
    14101 gggaaaaagc caaggcagcg tattaaaagc caatgcattt ggtcaggtaa tcgtagacca
    14161 acaaatactg caacatattt ttgtagcggg tgactgtgca tactatcagt cgattggttc
    14221 taatgcttta actgcccaat cagcggttcg caaaggaaaa ttagcagccc gtaatctttt
    14281 aaggcattca ggcttgctta aaatacttga gccttatcta caccgagata tcggctatgt
    14341 agttagtctt ggacctgcag atgcgattgg atggttagcc ttagaaaata atgtggttac
    14401 aggcttagca gcttatacta tcaaagaaat tgtagaagct cagtatgatt tattgctagc
    14461 aggaatcgat acatatctac tataaaaata tgatgcaggg agttatttaa tgttcaattt
    14521 gtttgatgat aagcctgttg caggaaaggc cagcacagtg attcagtcgc cagatgacaa
    14581 gctatttaat tttattggcc acgaactgct gacgaattac cctcgttggt cccctgaagt
    14641 aaaagaactt gagaaactaa ccgattctcc gttgggatta ggaacactgt gccgccaggt
    14701 tcgagttgat cagggaaatc gatcggagtc gacattcaaa atcgcggtat tcgaacccaa
    14761 caaacaaatt tgctttaatg gcgtgtcaaa cccgtttcgc tgtgattatt ccctcgagtc
    14821 aaaaaatcaa gaatcaactc aaattacctt tacttttgaa ttactaagct tagaactgca
    14881 tatgagaccc tttgaaaaac tcattcgaat tgccgtgcaa gatggtgcag aaaggacagt
    14941 tagaaacatt aagcgattaa ttgaattcaa cacgatttaa ttcagcttga aaagaataac
    15001 ccgaagaagc ttatacaacc ctttatattg aattggagaa aaagtatgga ttttatcgtt
    15061 gaaaaagatc ctggattaat tccacaagta ttatctaaca tccttgattc ctgcgtaaat
    15121 ggcattactt tggctgatcc tgatcaggag gacttacccc ttgtttatgc aaataaagcg
    15181 ttcgaaacaa taaccggtta tacacaagct gaaaccattg ggaaaaattg tcgattcttg
    15241 caaggcaatg atcgagatca gcaagagcgt catcagttac gagaagctgt tttaaataag
    15301 caatccatcg agataacgct gagaaattat cgaaaaaacg gcgaaatgtt ttataaccat
    15361 ctcgttataa cgcctttgtt tgattcgaaa ggaaatgttt tatactacct cggtgtacaa
    15421 ttcgatgtaa cacagcagaa aaaagctgag gaagaaatta aacgccttac agagcagcta
    15481 gcgaaattat caaagtaaat taattttttt gattactact agcaaaacag gtcttaaatg
    15541 agtatcatca gcgcaaatca aatgtgactt accatgacaa gttacagttt ttattgattg
    15601 acttcatcaa ataaccggaa gcgtggccga gcggtttaag gcactggtct tgaaaaccag
    15661 cgatgggcaa ctatccgtga gttcgaatct caccgcttcc gccaatcatt cactatccat
    15721 cccgttttaa ccccaatccg ctcccatcaa aacatttctg gtttaaagcc tagttttcgg
    15781 gatcgctgca cagacaactc gattttctgt atccaggaat aacatttgag tacgattcca
    15841 ggttcgaatc ctaactggac aattcgctac aaaatattat ttctccctcg gataaacaaa
    15901 tcgcaaagcg gctatcgagc aaaaatatgg atcaccctca acatgccaat catgaattct
    15961 taagttacac tcaccacttt atgaccatac gtcaaaatag agtgtcatga accaacaagc
    16021 gctttcttcc tttatctggt cggttgccga tcttttgcgc ggcgactaac aatccgaata
    16081 cggtaaggtg atcctgcctt tcacggtgtt gcgccgtctg gattgcgtac tggaatccac
    16141 caaggaagcc gtgctggcgg aatatgccga taagcagaaa gccggaatca atccggagcc
    16201 ttttttgttg cgcaaggcgg gccaaagttt ctacaacacg tcgcccatgg acatgaagaa
    16261 gttgatgggc gatcaagatc acatcaagga aaacctgtac gcctatatcc agggttttgc
    16321 gcctgcggta cgggatattt tcgagcgctt tgatttttat acacagattg accggcttgc
    16381 caaatccggg ttgttgtatc aggtggccga gaaattcgcc aacatcgatc tgcatccgga
    16441 agtagtaagt aatagccaga tggggttggt atttgaggaa ttgatccgca agtttgcgga
    16501 aatctccaat gaaaccgccg gagaacactt caccccgcgc gaagtcattc gcctgatggt
    16561 taatttattg tttatcgagg atgacgaagt gctggccaaa cctggtgtgg ttcgcaccat
    16621 ctatgacccc accgcgggta ctggcggcat gttgtctgtg gcgggcgaat acctcgaaga
    16681 acacaaccca caagcgcggc ttaccatgtt cggccaggaa cttaacgatg aatcctacgc
    16741 gatttgcaaa gcggatatgc tgatcaaggg ccaggatgtt gccaacattg tggccggtaa
    16801 tacccttgcc gacgatggcc atccgcacaa gaaattcgac tacatgcttt ctaatccacc
    16861 gtttggcgtc gaatggaaga aagttgaaaa ggaaatccgc aaggaatttg aatcgcaagg
    16921 ctacaacggg cgcttcggcc ccggtttacc cagggtatcg gatggttctc tgctgttttt
    16981 gctgcacctc atcagcaaaa tgcgcccggc ccatgaaggc ggcagccggt ttggcattgt
    17041 gctgaatggc tcaccgctat ttaccggtgg cgctggcagc ggcgaaagcg aaatccgccg
    17101 ctatgtactg gagaacgatc tggtggaagc gattatcggc ctgcccaccg atatgttcta
    17161 caacaccggc atttccacgt atgtctggat actctccaac cacaaaccgg aacaccgaaa
    17221 aggtttagtg caattgatcg atgccagcgg tttctggcaa aagatgcgca agagcctggg
    17281 cagcaagcgc aaggaattct ccgaagcgca tatcgctgag attacccact tgttcggaca
    17341 tttcgaagaa gccagcaagg atggtgcgcc gattagccgc atattcagga atgaagcctt
    17401 cggatatcgc accatcacgg tagaacgccc actacgtgac gcaaacggtc agatcgtgct
    17461 gggagaaaaa ggaaaactaa agggcaaacc gcagccggat agcagcttgc gtgacaccga
    17521 gaatgtaccg ctctccgaag atgtggacgt ctatttcaaa tgggaagtgc tgccgcatgc
    17581 tccggatgcc tggatcgatc acgagaaaac caagatcggc tatgagattc ccttcaaccg
    17641 gcatttctat gtattcaaac cgccgcgcga actggcagag atcgatgccg acctgaaaca
    17701 atgcaccgac cggattctgg cgatgatcaa aggattgtcg gcatgagttt cccgcgttac
    17761 gagcactata aggatagcgc cgtggagtgg ctgggtgagg tgccggagca ttgggaaatt
    17821 aagcatctaa aacattcttg ccaaatatat ccaagcaatg tcgataaaaa atcttatgag
    17881 aatgaatcgc ccatcttgct atgtaactat acggatgtgt attacaacga aagtattact
    17941 aaccaactgc aattcatgtc ggctactgct tcagctgatc agatcaacaa gtttagcctt
    18001 aaagcaggtg acacaattat taccaaggat tctgaaacag ccgatgacat tgcaattgcg
    18061 gcttatgttc cagaagattt gccgggtgtc atttgtggct atcatctggc attagttaga
    18121 ccattcccag aggcttgtgg tgcattcata aaacgacttt ttgattcaca ttatgcaaag
    18181 gcaagtttta atgtattggc taacgggctt acccgcgtag gacttgggca atatgcttta
    18241 aacaacttag aaataccttt tcctccctat atagaacaat ccatcattgc cactttcctc
    18301 gaccgcgaaa ccgccaaaat tgatgcgctg gtagccgaac agcaagggct gattgaattg
    18361 ctgaaagaaa agcgccaggc ggttatctcg catactgtca ccaaagggct gaacccggat
    18421 acgccgatga aggattctgg tgttgagtgg ctgggggaaa taccggaaca ttgggaacta
    18481 aaagcaacaa agaggttatt ccgaatggtt gtcgagcccg caccggatga caacgaccat
    18541 gaacttctgt cgatttacac ggcgatagga gtccgcccga gaaaagagct tgaggagaaa
    18601 ggcaacaaag ctaccacaac agatggatat tggatggttc ggaaaggaga tctaatcgtc
    18661 aacaaacttc tagcatggat gggagcgatt ggaatttcag attatgacgg tgtaaccagt
    18721 ccagcctatg acattctccg ccagaggatt ccgttagaac ctcgttatta tgattatctg
    18781 ttccgatgcg gaatctgttt ttcagagttc cgacgatatt ccagaggaat catggacatg
    18841 cgattgcgac tttatttcga agagcttggg caattgcaca tgccatttcc tcctgagttg
    18901 gagcaacagc agattgttag ctttctccaa gaagagcttg tgatattcga tgaactgacc
    18961 gccgaagccc aacgcgccat caccctgcta caagagcgcc gcaccgcgct catttccgcc
    19021 gccgtcaccg gaaaaattga tgtgcgcggc ctggtatcgg acaaacgggc tgcggaataa
    19081 accgcgtgaa caatctgcgc cctgaatccc ttttccatca tccgtattgt catgcgcatc
    19141 aaaacaccta taatggctga attagctatt tggaggattt
//
LOCUS       DAOQLJ010000027        19026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1006_length_19026_cov_7.132307, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000027 DAOQLJ010000000
VERSION     DAOQLJ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 19026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19026
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1006_length_19026_cov_7.132307"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PK522_03470"
     CDS             complement(<1..307)
                     /locus_tag="PK522_03470"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNC40824.1"
                     /translation="MTDACDLSNKHCKPCEGGVPPLSQREIDSLLQQLEQWEYRNNTI
                     HKDFTFKNYYQTMAFVNAVAWLSHREDHHPDMVVGYNHCQIVYMTHAIQGLSENDFIC
                     "
     gene            complement(325..1575)
                     /locus_tag="PK522_03475"
     CDS             complement(325..1575)
                     /locus_tag="PK522_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNC40825.1"
                     /translation="MQTFTAIFVTALLLTTLIELWLAIRHARHVWSNRNQVPNDFATQ
                     IDLASHQKAADYTCAKTRFNYPTIIMHSLLLLIFTLGGGLNVISNFWAGWIENPIFHG
                     MALILTTIFILSAIEIPFNYYRTFVIEAQFGFNKMSPAMFFSDLIKQYSLGLILGGLL
                     LFCMLWLMEKAGAYWWLYAWMAWMGFNVLLLSIYPTWIAPIFNKFAPLEDNALKTRIE
                     QLMQKCGFKSSGLFVMDGSRRSSHGNAYFTGFGKNKRIVFFDTLLSRLEGPEIEAVLA
                     HELGHFKLHHVLKRIVWSFIVSLVFFALLGFLMQQAWFYEGLGVSANTPSTAAMALLL
                     FFLIMPVFTFLLHPLSSLYSRKHEFEADAYAAKHASANDLIQALVKLYQDNAATLTPD
                     PLHSAFYDSHPPAATRIMQLKKLA"
     gene            1681..2229
                     /gene="orn"
                     /locus_tag="PK522_03480"
     CDS             1681..2229
                     /gene="orn"
                     /locus_tag="PK522_03480"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HNC40826.1"
                     /translation="MAQDSNNLIWIDMEMTGLNPDSDRIIEIALVITDSQLNTIAESP
                     VLAIAQPISILDSMDKWNRSTHAKSGLIDKVKASTLSEVEAEQIMLKFLQPHVPSAIS
                     PMCGNSICQDRRFLVRSMPKLEAYFHYRNLDVSTLKELAKRWKPTIMSGLNKESKHEA
                     LSDIYDSINELKYYREHFINIG"
     gene            2255..4810
                     /gene="glgP"
                     /locus_tag="PK522_03485"
     CDS             2255..4810
                     /gene="glgP"
                     /locus_tag="PK522_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNC40827.1"
                     /translation="MINKTVFTITINPKIPPRLSRLEELANNLWYSWDRSTRTLFSRI
                     DSRLWEAVGHNPKSFLKRVDESLLHKAASDQTFLASYNSVLSAYDSYHSEPARYHRFE
                     GLESRDQIAYFCFEFGFHESLPVYSGGLGILAGDHCKAASDLCLPFVAVGLLYRQGYF
                     SQTIDSQGNQRAIYNDSDFGDLPVEPALHDDGAEVHINIDLPGRSVNVKVWRVIIGHV
                     SLFLLDTDLPDNSAADRLITHQLYGGDKIMRIQQEILLGIGGVRALQAIGVKPTVWHS
                     NEGHAAFMMLERARNLVQHGLDFASALEAVATNTVFTTHTAVPAGHDHFPQTMMQEYF
                     GLFCNDLKIAWSEFMALGNMPGNEDFNMTALAIRLSRTHNGVSKIHGDVSASICRDLW
                     PQIEPEENPISYITNGVHVPTFLAQEWSVLFDRYLGHEWRNKMCEPDFWSQIDAIPDH
                     LFWSVRQTLKSQLFYGIRARLTEQNARNRGSEAHLDRLFKWIDPNNPNILTLGFARRF
                     ATYKRATLLFENLDWLRKILLDQDRPVVLIYAGKAHPADIPGQDLIRRISHVSRMPEF
                     EGRILLVEGYDLRLARRLVAGVDVWLNSPIFPLEASGTSGMKAGINGTINLSVLDGWW
                     GEGYDGKNGWAVKPGSENTEAVLRDQDESQSFYEILQDQVVPLYYRRNGFGYPAEWVK
                     MAKYSMASLLPRYSANRMVSEYVEKFYIPATNKGAQFVNDDYAIARNVAVWKAKIRKA
                     WDQVSLRRLDAPAKQINFGDLLNFKVAVKLNGLQPEDVMVELLLCRQYKKMRLTNFKR
                     FKFDCVGMVSPQEHLFELNHAPELCGRQEYYLRIYPYHSALTHPLEMGMMIWL"
     gene            4992..6041
                     /locus_tag="PK522_03490"
     CDS             4992..6041
                     /locus_tag="PK522_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40828.1"
                     /translation="MAINHEQINFDQSSFNQIKQSLQQAIKDKANSLRDNHAVTGVSS
                     FKEVLSKTVQDLLSSPSEKAIKNGSSQNTHALTLDRPIASSRDVQPIQKLISSAADYL
                     STRLQNEEKHTTELATEKALNNTAVSSFLPINAGQNIAQKISSEESTHESNTGLSSAT
                     WSGGKPDSIKELLDFMHTRGIAMNVGEASDFLYGSVGANDDLRDFNTILNAESPTTAN
                     NQALGQLFSNPSARINPNYATRSAEEVVAQHGNLLVRESKNGAQILAAQAANGMPLTD
                     ISNSATGVGGGIARYGITDANVQAILDNPKVSDDIKAQLKSYLGSGYQQNPTNYLLDI
                     NLASFATTGVFNQSK"
     gene            6202..7443
                     /locus_tag="PK522_03495"
     CDS             6202..7443
                     /locus_tag="PK522_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC40829.1"
                     /translation="MRILFIHQNFPAQFKYLAPTLAKQHQVRILTLNARLADNWQGIP
                     VNHYALQKASTKGIHPWIIDFEAQVIRGEATLYAAFNLRQQGFTPDVIIAHPGWGESL
                     ALKDVWPDAKLGLYCEFFYRAEGADVGFDPEFPNTDPALASRIRLKNTNTLIQLSTFD
                     TGISPTDWQASTFPELFRNRISVIHDGIDSTLLQPNPSATLTLKINSGDLLTLSREDE
                     IITFVNRNLEPYRGYHTFMRCLPRLLKQRPQARVLLVGGDGTSYGAKPLGSKSWKERF
                     IDEVHPQISEADWSRVHFMGNLPYQHFVTLLQCSSLHVYLTYPFVLSWSLLEAMSIGC
                     AIVASDTSPVRDAIIHNETGRLVNFFDYKALADEISSLLDNPDERQRLGANARALIYE
                     KYDLKTICLPKQMEWVENILK"
     gene            complement(7513..8604)
                     /gene="ychF"
                     /locus_tag="PK522_03500"
     CDS             complement(7513..8604)
                     /gene="ychF"
                     /locus_tag="PK522_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNC40830.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIDPNVGIV
                     EVPDPRLIKLSEIVKPKVVQSATVEFVDIAGLVAGASRGEGLGNKFLANIRETDGIVH
                     MVRCFANDNIVHVAGRIDPISDIEVIQTELALADLTTVEKAVQRESKLAKSGNKESIR
                     NVTVLEGLHNHLDQNKPARSFKFEDAQHALIKSACLLTMKPTIYVTNVADTGFQDNPL
                     VKSVQDYAAQEGAPVVVICASLEAEIADLSDDDKQVFLADLNLTEPGLNRLIRAAYQL
                     LGLQTYFTAGVKEVRAWTFHSGDTAPQAAGVIHSDFERGFIRAEVIAYDDYVTYNGEQ
                     GAKEAGKMRLEGKDYIVQDGDVVHFRFNV"
     gene            complement(8674..9243)
                     /gene="pth"
                     /locus_tag="PK522_03505"
     CDS             complement(8674..9243)
                     /gene="pth"
                     /locus_tag="PK522_03505"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNC40831.1"
                     /translation="MKLIVGLGNPGEKYALTRHNIGFIWLQQLAAELKVSFVSEAKFH
                     GLCASGIYQGEPFRLLMPQTYMNASGMAVASLCRFYQCNPEQILVIHDELDLPPGTIK
                     LKLGGGSGGHNGLKDIITRLGDKDFWRLRIGIGHPGDRAAVVDYVLRPPSKQEAVLID
                     ESISRSLTLCQLLLSGDYMAAMQKLHSSQ"
     gene            complement(9400..10014)
                     /locus_tag="PK522_03510"
     CDS             complement(9400..10014)
                     /locus_tag="PK522_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNC40832.1"
                     /translation="MQIEIDANNRAMQGKGASRRLRRDGKVPGIVYGGEGQAQSIELD
                     HNNLFHKLKLEAFHASILSLNLDGKKEQVLLRDVQMHPYKQQVLHVDFQRVDQNRKIH
                     MKVPLHFINAEIAPGVKLSGGLISHVLTEVDISCLPKDLPEFITADLSEMTAGSTLHL
                     KDLKLPNGVEIIALSKGDDLPVATLTVKRAEEGAEEGSSATPAA"
     gene            complement(10084..11034)
                     /locus_tag="PK522_03515"
     CDS             complement(10084..11034)
                     /locus_tag="PK522_03515"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNC40833.1"
                     /translation="MSYDSLMVFTGTAHPKLAQDVVKYLNIHLGRATVSRFSDGEIMV
                     EILENVRGKDVFVLQSTCVPTNDSLMEILVIVDALKRASAGRITAAIPYFGYARQDRR
                     PRSARVAITAKVVANMLTTVGIDRLLTMDLHSDQIQGFFDIPVDNIYGMPILLGDVWK
                     HNYQNLIVVSPDVGGVVRARHMAKRLECDLAIIDKRRPKPNESKVMNIIGEVKGRTCV
                     IIDDMVDTANTLCEAARALKDEGAQSVVAYSTHAVLSGKAVERIASSQLDKIVVTDTI
                     PLSDEAKACDRICQLSIANLLGETMLRISNESSLSSLFME"
     gene            complement(11162..11236)
                     /locus_tag="PK522_03520"
     tRNA            complement(11162..11236)
                     /locus_tag="PK522_03520"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11202..11204),aa:Gln,seq:ttg)
     gene            complement(11244..12083)
                     /gene="ispE"
                     /locus_tag="PK522_03525"
     CDS             complement(11244..12083)
                     /gene="ispE"
                     /locus_tag="PK522_03525"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNC40834.1"
                     /translation="MQFYPAPAKLNLFLHIIGRRDDGYHLLQTIFRFIDLNDRLGFNL
                     RNDGVIQLQTPLAGVAAEQNLCIRAAKLLQEEGSVNQGVDIYLEKHIAMGGGLGGGSS
                     DAATTLIMLNSLWGLNWHRDRLMELGLKLGADVPVFIYGDNAFAEGVGEKLSAVNLPN
                     AWYLVLMPPVHVSTAQIFASNELTRNTIPITIPPFSIRDGHNDLEPVVCRMYSDVAKC
                     IDWLKKLDNTTLVAMTGSGASVFAEFETEAQAKEAYLRLPDGVNAVVAKGLTKHPLKD
                     FYR"
     gene            complement(12131..12772)
                     /gene="lolB"
                     /locus_tag="PK522_03530"
     CDS             complement(12131..12772)
                     /gene="lolB"
                     /locus_tag="PK522_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HNC40835.1"
                     /translation="MIIRVQETIYFVSCSILLSTILLLGGCASLEKHSVVKTIVLEPE
                     AEKARDLTNFDLIGRISVKTNNDGFSGGIRWQHKNHFHHLLLLSPIGQVVAQIAQDDQ
                     GIQLITSDQKIYYAASIEALTEQVFGWKLPIIGLEYWIMGRANPLIVAEIDMDRDDQV
                     IAIRQDGWEITYLDYSSRSVPQHNKVKSAPRIIELNYPNLKLKLVIDSWKNIN"
     gene            complement(12813..13091)
                     /gene="minE"
                     /locus_tag="PK522_03535"
     CDS             complement(12813..13091)
                     /gene="minE"
                     /locus_tag="PK522_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HNC40836.1"
                     /translation="MSLLDYFRSSKSKTASIAKERLQILVAHERYYRNKPSYLPQLQE
                     ELLEVIRKYVNVDQDAISVKFEQDENQETLELNIVLPESQNNKSPLEI"
     gene            complement(13091..13900)
                     /gene="minD"
                     /locus_tag="PK522_03540"
     CDS             complement(13091..13900)
                     /gene="minD"
                     /locus_tag="PK522_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNC40837.1"
                     /translation="MARIIVVTSGKGGVGKTTTSAAIAMGLAKRGHKTAVIDFDVGLR
                     NLDLIMGCERRVVYDFINVINGEANLSQALIRDKNCNLLYILPASQTRDKDALSIEGV
                     GKVLEELAKDFQYIVCDSPAGIEKGAYLAMYYADDAFVVTNPEVSSVRDSDRMLGILA
                     SKSRRAELGMEPIKEFLLLTRYDPNRVESGEMLSLEDVQEILSLHLLGIIPESKSVLT
                     ASNAGVPVILDEKSDAGMAYADVVARYLGEKRPHRFIESKKGFLRKLFGGK"
     gene            complement(13927..14682)
                     /gene="minC"
                     /locus_tag="PK522_03545"
     CDS             complement(13927..14682)
                     /gene="minC"
                     /locus_tag="PK522_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNC40838.1"
                     /translation="MSLDVNKNYDMQPVLEFKSSTFFVLVLIIHSNDLMMIEQKLREK
                     TSQAQDFFRNSPIILDVRELNQQSHGIDIIKLINLIRSIGLFPVGIRGGNDQQNLQAA
                     SLFIPVDMSQSSASSQAEIQKTATSSPSAAPHEVIKEIVVSTATTLINQPVRSGQRIY
                     ASGDLIILTQVSAGAEIMAEGNIHVYNTLRGRALAGVQGNRDARIFCSDLQAELISIA
                     GDYKTSEDLKDITPRKPVQIYLQDQALIIKELI"
     gene            14938..15483
                     /locus_tag="PK522_03550"
     CDS             14938..15483
                     /locus_tag="PK522_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HNC40839.1"
                     /translation="MRKILSLICYLFLLSACTSLPDVIKNIPVIDVPYGVVAKDASTY
                     KNKQVRWGGVIIQTETEKELSFVQILFYPLDSSGYPLLNQEPEGRFIIASTDFLDPAI
                     YTKDRVITVAGMLNGGVERSIGNKKISIPLLLTKAIYLWPKDYRKSYISDGRYPYFYY
                     PGFYGYPFFYPGYYGPYRYWR"
     gene            15492..16763
                     /locus_tag="PK522_03555"
     CDS             15492..16763
                     /locus_tag="PK522_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40840.1"
                     /translation="MQKLIRIAGLSFFFLLLILAALLLLITDSHPVVKRDADINHNHV
                     DRIKQIFDNHRNHIYPGELGTAKIASEDVDAVVNYFTYLLTKGSTQIEITDRQMLFRL
                     SIPLSMGITTSYLNLETNFIEVEGFPSLQSIKISYFQLPVFLITKIFPKLAEWSISII
                     PEIQLAVSALQQVRMSPSGIDIVYRWQGGLFDKLNKPFAMSLPLVNKQELDRFYRYHF
                     ILTNFTASKSSPIPLSQLLIPVMRLASEQSTKGNAIEENRAAILITSLHILGISLKTL
                     FPEAINWPKQQKYRVTLDGREDFAKHFILSAAITAYADTKLSDAIGLYKEIEDARSGS
                     GFSFNDIAADRAGTKFGEKAVENSMSALKIQEKISSGLDDKDLMPAWSDLPEFLTEAV
                     FKERFGDINTPSYLQIMDKIEQRIANLIIFH"
     gene            complement(16767..18482)
                     /locus_tag="PK522_03560"
     CDS             complement(16767..18482)
                     /locus_tag="PK522_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC40841.1"
                     /translation="MKSKTFLFLLLIGLAGCNQLPKVGSADSARLAKDSQSQIDSPKQ
                     ELTSELLFDYLLAETALQRENIDVAIQSYSRLAKNTQDPRIAERATDIALRARRPDAA
                     QEAVARWVNLEPNSMNARQAAAALFVSMGKLDKAKPHLEKLLSSEKSSVDKAFMHLNK
                     LLSRHPQKAETYKLVQSLAAGYPNLPESHFAVSQAAWSNNQLDIALKEMKRALELRPD
                     WEIAAIHQGRILQQIANAEAMTFYENYLGKYPKANDMRIAYVRMLMTEKNFPLAREHF
                     QKMMAENPVNPDIAVAVGLLSLELNDFEGAEENFKKALELGYEDNNNIYFNLARIYEV
                     TQRTDDALETYQRISGGERFFPAQIRYAALIAKKEGINRARQHLHQIPTETDQQRIQL
                     MLAEAQLLREVGKHQDAFNLLDKNLARNPDQPELLYDRALAADKIDRFDIVEKDLRRL
                     IELKPDNAHAYNALGYSLAERGIRLAEALSLIQKAVELSPDDPYIMDSLGWVYFRMGK
                     IQEGLNYLNLAFSSRPDPEIAAHLGEVLWVQGERENAEKIWRSALETDPDNETLLDTM
                     KRLMQ"
     gene            complement(18638..>19026)
                     /locus_tag="PK522_03565"
     CDS             complement(18638..>19026)
                     /locus_tag="PK522_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNC40842.1"
                     /translation="RYLRKIYMDPMTNSFEWGLIKQGEKGITGVYSLSTKIPIKRANF
                     PEQYAAFSKAKSYQDWKFNHASGTTGQGRQQTQPNAPPGSPGQSQNTNPFTGQSTPSG
                     QNRGGPANPFPQNQPDNSRANPFSTQ"
BASE COUNT         5493 a         3877 c         3868 g         5788 t
ORIGIN      
        1 cacaaataaa atcattttct gatagacctt gtattgcatg cgtcatgtat acgatctggc
       61 agtggttata gcctaccacc atatcgggat ggtgatcttc ccgatgagat aaccaggcaa
      121 ccgcatttac aaatgccata gtttggtagt agtttttaaa tgtaaagtct ttatgaatcg
      181 tattgtttcg atattcccat tgttcaagtt gttgcaagag gctatcgatt tctcgctgcg
      241 ataagggagg tacccctcct tcacacggtt tacagtgttt attggaaaga tcacacgcat
      301 cagtcatggt attctccttg ttggttaagc aagttttttt agctgcataa tccgagttgc
      361 tgcaggggga tgcgaatcgt aaaacgcaga atgcagggga tccggtgtca gcgttgccgc
      421 attatcttga taaagcttaa cgagagcttg aattaaatca tttgctgaag catgttttgc
      481 tgcataggca tccgcttcaa attcatgctt gcgtgagtaa agactcgata aaggatgcag
      541 taagaaggtg aacacgggca tgattaagaa aaagagtaat aatgccatcg cagccgtgga
      601 aggagtgttg gcagatacac ctagcccctc ataaaaccac gcttgttgca ttaaaaagcc
      661 gaggagcgca aaaaagacaa ggcttactat gaatgaccat acaatgcgct ttagtacatg
      721 gtgaagtttg aaatgaccta attcatgcgc aagaacggcc tcaatttcag gcccttctaa
      781 gcgagacagc agcgtatcga aaaagacgat gcgtttattc ttgccaaacc cagtgaaata
      841 ggcgtttccg tgactgctac ggcgtgatcc atccattaca aacaatccgc tggatttgaa
      901 accacatttt tgcatgagct gctcaatccg tgttttaagt gcattatctt caagtggtgc
      961 aaacttgtta aaaatcggag cgatccaggt tggataaatt gagagtagca agacattaaa
     1021 gcccatccaa gccatccaag cgtataacca ccaatacgct cctgctttct ccattaacca
     1081 caacatgcaa aataacaata aaccacccag aatgagtccc agtgaatatt gtttgattaa
     1141 atcagaaaaa aacatggcag ggctcatttt attaaagcca aactgtgcct cgattacaaa
     1201 ggttcggtag taattaaaag gaatctcgat cgcactgagt ataaatatcg ttgttaagat
     1261 cagtgccatg ccgtggaaaa tgggattctc tatccatcca gcccaaaagt tggaaattac
     1321 atttaaccct cctcccagcg taaatataag cagtagaagt gaatgcatga ttatggttgg
     1381 atagttaaag cgcgttttag cgcaggtata atcggctgct ttctgatgag aagccaaatc
     1441 aatttgtgta gcaaaatcat tcgggacttg gttgcggttt gaccaaacat gacgtgcatg
     1501 acgaatcgcc agccatagtt caatgagtgt cgtaagtaat aatgcagtaa caaaaatagc
     1561 agtaaaagtt tgcatagtgg ataaataaaa aagattaatc acacacgccc atttacgcaa
     1621 aagtgacgtg taactcgcaa tatgacacaa tccgcttaat ataattcacg aaggctcatt
     1681 atggcacagg atagcaacaa cctcatctgg atcgatatgg aaatgacagg attgaatcct
     1741 gatagcgatc gcattatcga aatcgcttta gttattacag attcacaatt aaataccatt
     1801 gctgagtctc ccgttttggc tattgctcaa cccatttcaa ttctcgatag tatggataaa
     1861 tggaatcgtt ctactcatgc caaatctggg ctgattgata aggttaaagc atcaacgctc
     1921 tctgaagtcg aagcagaaca aattatgttg aaattcttgc aacctcatgt gccttctgca
     1981 atatcgccga tgtgcggtaa ttcgatttgc caggataggc gattcttggt tcgttccatg
     2041 cctaaactcg aagcttattt ccattatcgg aatctcgatg ttagtacgct caaagagcta
     2101 gccaagcgct ggaaacccac aattatgtct ggtctaaata aagaaagtaa gcatgaagcc
     2161 ctatcggata tttatgattc aatcaacgag ttgaaatact atcgtgagca tttcatcaat
     2221 atcggatgat cagctttcaa tgaaggagta cggaatgatt aataaaacag tgtttacaat
     2281 aacaattaac cctaagatac ctccacgtct gtcgcgcttg gaagagttag caaataatct
     2341 ctggtatagt tgggatcgaa gtacgcgtac attgttttct cgcattgatt ctcgattatg
     2401 ggaagcggtt ggacataatc ccaagtcatt tcttaaacgt gttgatgaat cgctacttca
     2461 taaagcagca agtgatcaaa cttttttggc tagctataac agtgtattgt cagcttatga
     2521 ttcctatcat tcagagccgg ctcgatatca tcgatttgaa ggacttgagt cgcgcgatca
     2581 gattgcctat ttctgctttg aatttggttt ccatgaaagc ttaccggttt attcaggtgg
     2641 attaggtatt ctagcgggtg atcattgtaa agcagctagt gatttatgct taccgtttgt
     2701 tgccgtaggg ttgctgtatc gacaaggtta tttttcccag acgattgata gccaaggtaa
     2761 tcaacgtgca atttataatg attccgactt tggtgattta cctgtagaac ccgcgcttca
     2821 tgatgatgga gctgaagtgc acattaacat tgatttacct ggccgaagcg ttaatgtcaa
     2881 agtttggcgt gtcattattg gccatgtcag cttatttctt cttgatactg acttgccaga
     2941 taactctgct gctgaccggc ttatcacgca tcaattgtat ggtggcgata aaatcatgcg
     3001 tatccagcaa gaaattctac tggggattgg gggtgttcga gctttgcagg ctatcggggt
     3061 caaaccaact gtatggcaca gcaatgaagg tcatgctgca ttcatgatgc tagaacgtgc
     3121 ccgtaatcta gttcaacatg gtctcgattt tgcaagcgcc ctcgaagcag tagcgaccaa
     3181 tacggttttt actactcaca ccgctgttcc ggctggacat gaccactttc cccaaaccat
     3241 gatgcaagaa tattttggtc tattttgtaa tgatctaaaa attgcgtgga gtgagttcat
     3301 ggcgttgggt aatatgcccg gaaatgaaga cttcaacatg accgcactcg cgattcgctt
     3361 atcgcgaact cataacggtg ttagcaagat tcatggggat gtttctgcca gtatatgtcg
     3421 cgatctttgg ccgcaaatcg aaccagaaga aaatccaatt tcatatatca ccaatggcgt
     3481 tcatgtacct actttcttgg ctcaggaatg gtctgtctta ttcgaccgct atctgggcca
     3541 tgaatggcga aataaaatgt gtgaaccaga tttctggtcg cagatcgatg ctatccctga
     3601 tcatctattt tggagtgtta ggcaaacact caaatcacag cttttttatg gaattcgagc
     3661 ccgattgacg gaacagaacg ctcgaaatcg tggatccgaa gcgcatctcg atcgactctt
     3721 taaatggatt gaccccaata atcccaatat tttgacgtta ggatttgctc gccgatttgc
     3781 tacctataag cgtgcaactc tgttatttga gaatctcgat tggcttcgca aaattttgct
     3841 cgatcaagat cgcccggtcg tgctgattta tgctggtaaa gcgcatcctg ctgatattcc
     3901 tggtcaagat ttgatccgtc ggattagcca tgtgtcacgc atgcctgagt tcgaagggcg
     3961 aattttatta gtggagggct atgatttaag attggctcgc cgactcgtgg ctggtgtcga
     4021 tgtttggttg aatagtccta tttttccgct ggaagcaagt ggtacttcgg gtatgaaagc
     4081 cggtatcaat ggcacaatta atttgagtgt gctagatggc tggtggggag aagggtatga
     4141 cgggaagaat ggctgggcag ttaagcctgg atctgaaaat acagaagcag tattacgaga
     4201 tcaggatgag agtcagtctt tttatgagat tttacaagat caagttgttc cactctatta
     4261 tcgtcgtaat ggttttggct atcctgcaga atgggtcaag atggcgaaat attccatggc
     4321 ttcactatta ccccgttata gcgccaatcg catggtcagc gaatatgtag agaaattcta
     4381 tattcctgca accaataagg gcgcgcagtt tgtaaatgat gattatgcaa ttgctcgaaa
     4441 tgttgcagtc tggaaagcaa agattaggaa agcctgggac caagtttcat tgagacggct
     4501 ggatgcccct gcgaagcaga tcaactttgg tgaccttttg aattttaagg ttgcggttaa
     4561 gctaaatggt ttacagccag aagatgtaat ggttgaactg ttactttgtc gtcagtataa
     4621 aaaaatgcgt ttgaccaatt tcaaacgttt caaatttgat tgtgttggaa tggtttcgcc
     4681 acaggagcat ttgtttgagt taaatcatgc acctgaactt tgtgggaggc aagaatatta
     4741 tctccgaatc tatccttatc actcagcatt aactcatccg cttgaaatgg gtatgatgat
     4801 ctggttataa aatatagtgc taggctacag atcatatttg ctagtctagt actattaaaa
     4861 taaatctgca tggattggtc taaatttgag atcattttat gtgatttgct cattttttat
     4921 tttagagtgg cacataattt gcatagtatt agctgacaac attaatagga tcagttgaac
     4981 taggaggata tatggctata aatcatgagc aaataaattt cgatcaatcg tcatttaatc
     5041 agattaagca aagcttacag caagcgatta aagacaaagc caattctttg cgggataatc
     5101 atgcagtaac tggagtttcc tcattcaaag aagttttgtc aaaaactgtg caggatcttc
     5161 tatctagccc cagtgagaaa gcaattaaaa acggatcatc acaaaatacc catgcgttaa
     5221 cgctcgatcg ccccatcgca tcttcgagag acgttcaacc tatccagaaa ttaatcagtt
     5281 ccgcagcgga ttacctctca acgcgtttac aaaacgagga aaagcatacg acggaattag
     5341 cgacagaaaa agcactaaat aatacagcgg tgagtagttt cttacccatt aatgcgggtc
     5401 agaatatcgc tcaaaaaatc tcatctgaag aatccacaca tgaatcaaat acaggattga
     5461 gctcggctac gtggtctggt ggaaagccgg atagtattaa ggagctgttg gatttcatgc
     5521 atacccgtgg tatagcaatg aatgtgggcg aagcttctga tttcttgtat ggcagtgttg
     5581 gtgccaatga tgatcttcgt gattttaata cgattctgaa tgcagagagc ccaactactg
     5641 caaacaatca agcattgggg cagttgtttt caaacccctc tgcaagaatc aatccgaatt
     5701 acgctactcg tagtgctgaa gaagtggttg cccagcatgg caatttgtta gtaagggaaa
     5761 gtaaaaatgg agcacagatt cttgcggctc aggcagccaa tggcatgcca ctcaccgata
     5821 tttctaatag tgcaacagga gtaggaggtg gtattgctcg atatggcatc actgatgcga
     5881 atgtgcaagc aatattagat aaccctaagg tttctgatga cattaaggca cagcttaaat
     5941 cctacttggg tagtggatat cagcaaaatc caacgaatta tttgcttgat atcaatttgg
     6001 cttcatttgc tactacaggg gtattcaatc aaagtaaata gctttgtcag tgtattattg
     6061 gtagtgcaac tgtcttagcg aaagtataat tggctaggat agttgcaatg ctcaaagata
     6121 gaaagaaatt aaagataaaa tagcttagaa gtagaataat ttggatccga agcggcaatt
     6181 attgccgcag atccggcaat aatgcgtatt ttattcatac atcaaaattt tcctgcacaa
     6241 tttaagtatc ttgccccgac ccttgctaag caacatcagg tcagaatact aaccttaaac
     6301 gctcgtctgg cagataattg gcaggggatt ccggttaacc actacgcgtt acaaaaagca
     6361 tctacaaaag gtattcatcc ctggatcatc gattttgagg cacaagtgat tagaggtgaa
     6421 gcgacattat atgcagcgtt taatttacgg caacaaggtt ttacacctga tgtcatcatt
     6481 gcccatccgg gatggggaga aagtttagcg cttaaagatg tttggcctga cgcaaaacta
     6541 gggctgtatt gtgagttctt ctatcgtgcg gaaggtgctg atgttggttt tgaccccgag
     6601 tttcctaata ctgatcctgc gcttgctagt cgaatacggc taaaaaatac caatacactt
     6661 attcagttga gcactttcga tactgggatt tcaccaactg attggcaggc gagcactttt
     6721 cctgagttgt ttcggaatag aattagtgtt atccatgatg gaatcgattc gacccttttg
     6781 caacccaatc ccagtgccac gcttacactg aagataaatt cgggtgattt acttaccttg
     6841 tcaagagaag atgaaatcat tacttttgtg aatcgcaact tggagccgta tcgaggctac
     6901 catacattta tgcgatgttt accaagattg ctcaaacagc gtccacaggc aagggtttta
     6961 ctcgtgggtg gcgatggcac cagttatggc gcaaaaccat taggatcgaa gagctggaaa
     7021 gaacgattca ttgatgaggt acacccacaa attagtgagg ccgactggtc ccgagtgcat
     7081 tttatgggta acttacccta ccagcatttt gttactttac ttcaatgttc ctcgctgcac
     7141 gtttatttaa cctatccttt tgtactttca tggagtttac tcgaagcgat gagtattggt
     7201 tgtgcaatcg ttgcaagcga tacttctcct gtacgtgatg caattattca taatgaaacg
     7261 ggtcgattgg tcaatttttt tgactataaa gcgcttgcgg atgaaatcag ttcattactt
     7321 gataatcctg atgaaaggca acgccttgga gctaatgcga gagcacttat ttacgaaaaa
     7381 tatgatctaa aaacaatctg cctcccgaaa cagatggagt gggttgaaaa tatcttgaaa
     7441 taagcttccc taaatcaggt aagaggggag tgagcttcta agttaaaaat aaaaagtcca
     7501 gctttgcctt tgttatacat taaagcggaa atgaacaaca tcaccatctt gcacaatata
     7561 gtctttacct tccaagcgca tttttcctgc ttctttcgca ccctgttcgc cgttgtaggt
     7621 cacataatca tcgtaggcaa tcacttctgc tcgaatgaag ccacgctcaa aatctgaatg
     7681 aatgacgccc gctgcttggg gtgccgtatc accgctatga aaggtccaag cacgtacttc
     7741 cttaacgcct gcagtaaaat aagtttggag tcctaagagc tgataagcgg cccgaatgag
     7801 gcgatttagt cccggttcgg taaggttgag atcggctaag aatacctgct tgtcgtcatc
     7861 tgataaatcc gcaatttcag cttccagaga tgcacaaata actacaaccg gtgcgccttc
     7921 ttgagctgca tagtcttgga cgctttttac caaagggtta tcttgaaaac ctgtatcagc
     7981 aacatttgta acgtaaatcg ttggtttcat tgttaatagg cacgctgatt ttatcagcgc
     8041 atgctgtgcg tcttcgaatt taaagctgcg cgctggttta ttttggtcaa ggtgattatg
     8101 caggccttct agaacggtta cgttacgaat gctttcttta ttaccggatt tagccaattt
     8161 cgattctcgt tgtacggctt tttcaacggt tgtaagatca gccaaggcaa gttcagtttg
     8221 aatcacttca atatcggaga ttggatcgat tctaccagcc acatgaacga tattatcgtt
     8281 agcaaagcag cgtaccatat gaacgatacc atcggtttca cggatattcg caagaaattt
     8341 gtttcctagt ccttctccac gagaagcgcc tgcaactaat ccagcgatat caacaaactc
     8401 aactgttgca gattgtacaa ctttaggctt aacaatctca ctgagtttta ttaaccgtgg
     8461 atctggcacc tcaacaatgc cgacattggg gtcaatggta cagaaaggat aattttcagc
     8521 tgcaataccc gctttcgtta aggcattgaa aagtgtagat ttacctacat taggtagacc
     8581 gacaataccg cattttaggc tcatatttag gcgtaattgt tagaagaaaa aattgctatt
     8641 tagtgtctgg gtataagctt gcaatcatta atattattgg ctgctatgca atttttgcat
     8701 agcagccata taatcaccgc tgagtaatag ctggcataaa gttaggctcc tgcttatgct
     8761 ttcatcgatg agaactgctt cttgcttact gggagggcgt agcacataat caactaccgc
     8821 agctctatcc cctggatgac caatccctat tcgtaatcgc caaaaatctt tatcacctag
     8881 ccgagtaata atatctttta acccattatg tcctcccgat ccaccgccaa gctttagctt
     8941 gattgtacct ggcggtaaat ccagctcatc gtgaatgacc agtatttgct cagggttaca
     9001 ttggtaaaaa cgacataacg aggcaaccgc cattccactt gcattcatgt aggtttgtgg
     9061 catcagtaat cggaaaggtt ctccttggta gattccactt gcacataagc catgaaattt
     9121 tgcttctgat acgaatgaaa cctttagttc agctgctagc tgctgtaacc atataaagcc
     9181 tatattgtgg cgagtcagag catatttttc acctgggttt cctaaaccta caattagctt
     9241 cattggttat taaacaaaat cagtaggcag ggtttattac tgaaaagtac aaaaccttgt
     9301 tgcttgccat cgaatagaaa atcgattcat tgatagtgca aggaacgaag ccaaccttgt
     9361 aaaggtagaa atcgttcctt gcgctagtgc ttgtcgttgc taggcagctg gggtcgctga
     9421 acttccttct tcagctcctt cttcagctcg tttaacggtc agtgtagcaa ctggtaaatc
     9481 atcgccttta cttaatgcaa tgatttcaac cccgttaggc agtttgagat ctttaaggtg
     9541 taaagtactt cctgcagtca tttctgataa atcagccgtg ataaactcag gtagatcttt
     9601 tggtaggcag gaaatatcaa cttccgttaa cacatggcta attaaaccgc ccgatagctt
     9661 gactccagga gcaatttcag cattgataaa atgtaaagga accttcatat gtatttttct
     9721 attttgatca acgcgttgaa aatccacatg caagacctgc tgtttgtaag gatgcatttg
     9781 cacgtccctt aatagaacct gctccttttt tccatctaga tttaaggaaa gaatcgaagc
     9841 gtgaaacgct tctaacttga gtttatgaaa gagattgttg tgatctaatt cgattgactg
     9901 agcttgccct tctccaccat aaacaatacc cggtacttta ccatctctgc gcaggcggcg
     9961 gctcgcaccc ttaccttgca ttgcccgatt atttgcatca atttcaattt gcatcctttt
    10021 tctcctaaaa ttactaaaca gattttatcc gcgaccagaa aaatcactaa taaaactact
    10081 tacctactcc ataaacaaag aactcaaaga gctctcatta ctaattctta gcattgtttc
    10141 acccaaaagg ttggcgatac ttagctgaca aatgcgatca caggctttgg cctcatcaga
    10201 aagtggtatt gtgtcggtta caacaatttt atcgagttgc gaacttgcaa tacgttctac
    10261 tgcttttcct gataaaactg catgcgttga ataagcaacg acgctctgag caccttcatc
    10321 cttcaaagcc ctagcagctt cacacagcgt attggctgta tctaccatat catcgataat
    10381 gacgcaggtt cgccctttaa cttcaccgat aatgttcatt acttttgatt cattgggttt
    10441 agggcgtctt ttatcaataa tcgccaggtc gcactcgagc cgctttgcca tgtgccttgc
    10501 cctcacgaca ccaccaacat caggtgatac aacaattaaa ttctgataat tatgtttcca
    10561 aacatcccca agcaatatgg gcattccgta gatattatcc actgggatat cgaaaaagcc
    10621 ttggatttga tctgagtgta agtccatcgt cagtaatcga tcgataccta cagtggttaa
    10681 catattagca actacttttg ctgtaatggc aacgcgcgct gaccgtggac gccgatcttg
    10741 ccgtgcataa ccaaaatagg gtattgcggc ggttatacgt cccgcagagg ctcgtttcag
    10801 ggcatcaacg attaccaaaa tttccattag gctatcgttt gttggtacac aagttgattg
    10861 aaggacgaat acatccttac ctcgaacatt ctcaagtatt tccaccatga tttcaccatc
    10921 actaaaccga gatactgtag ctcggcctag atggatatta aggtacttca ctacatcctg
    10981 agccaattta ggatgagccg tacccgtaaa aaccattaag ctatcataag acacaattta
    11041 ttatttttat atcaacggaa aacagatggg agtaaacaag ctttctttga aaacaattat
    11101 caggttaaag ataaaactat atgcaaataa ataaagctaa tactaagcaa tgaaccgtag
    11161 ttggctgggg aggtagggat cgaacctacg aatgccggaa tcaaaatccg gtgccttacc
    11221 acttggcgac tccccaatta tcattaacga taaaaatcct ttaacggatg tttagttaat
    11281 ccttttgcga ccacagcatt taccccgtct ggtaagcgaa gataggcttc tttagcttgt
    11341 gcttctgtct caaattccgc aaatacagaa gctccagatc cagtcattgc taccaaagtt
    11401 gtattatcta atttttttaa ccaatctata cattttgcca catcagagta catacggcaa
    11461 actactggtt caagatcatt atgtccatca cggatggaaa agggcggtat tgtgatcgga
    11521 attgtgttgc gcgtcaattc attacttgca aaaatctgag cggttgagac gtgaactggg
    11581 ggcatcagga ctaaatacca agcattaggg agattaactg cggaaagttt ctctcctaca
    11641 ccttcagcaa atgcattatc accataaata aaaactggaa cgtcagcgcc taattttaac
    11701 ccaagctcca ttaaacgatc cctgtgccaa tttaaacccc ataaagaatt aagcatgatt
    11761 aacgtcgtcg ctgcgtccga gcttccacca cctaaccctc cacccattgc aatatgtttc
    11821 tcaagataaa tatcaacacc ttgatttaca gaaccttctt cctgaagtaa ttttgcagct
    11881 cgaatacata aattctgctc tgctgcaacc cctgctagcg gagtttgtaa ttggatgaca
    11941 ccatcgtttc ttaaattgaa acctaatcga tcattgagat caataaacct aaagatagtt
    12001 tgtaatagat gatatccatc atcacgccga ccgataatat gcaaaaacaa attgagttta
    12061 gcgggtgctg ggtaaaattg catgttattt tgttagttca cagaatagca tcttatcact
    12121 gagatgatcg tcaattaatg tttttccagc tatcaatgac gagttttaat tttaggttag
    12181 ggtaatttaa ttcgattatt cgaggggcgc tttttacttt attatgttgt ggtactgagc
    12241 gagaagaata atccaaataa gttatttccc aaccatcttg ccttatcgca ataacttgat
    12301 catcgcgatc catgtcaatt tctgcaacga tcaggggatt cgccctgccc attatccaat
    12361 actcaagtcc tataatcgga agtttccaac cgaatacttg ctcggtcaac gcttcgatac
    12421 tcgctgcata gtatattttc tgatctgagg ttatgagctg aattccttga tcatcctgtg
    12481 ctatctgagc aacaacctgg ccaattggcg agagtagtaa cagatgatgg aaatgatttt
    12541 tatgctgcca acgaattcca cctgaaaatc cgtcattatt tgttttgaca gaaattcttc
    12601 cgatcaaatc gaaattagtt aagtcgcgtg ctttttctgc ctctggttct aaaacaatcg
    12661 ttttgacaac actgtgcttc tccaatgaag cacagcctcc aagtaacaga atcgtactta
    12721 acaaaatgct acacgataca aaatatatcg tttcttgaac acggataatc aaaatagcgt
    12781 tagtcgtttt tttatcagat aaccctgata tattaaattt ctaatgggct tttgttattt
    12841 tgagattcag gcaaaacaat attaagttcg agtgtttctt gattttcatc ttgttcaaat
    12901 ttgactgaga tggcatcttg atcaacattg acatattttc gtattacctc aagtaattct
    12961 tcttgcaact gaggtaaata ggaaggttta tttcgataat aacgctcatg cgctacaaga
    13021 atctgcaatc tttctttggc aatagacgct gttttagact tagatgatct aaagtaatcg
    13081 agtagactca ttattttcct ccaaaaagct tcctgagaaa tcctttcttg ctttcgataa
    13141 agcgatgtgg tcttttctca ccgagatatc gagcaacaac atcagcataa gccatacccg
    13201 catcactttt ttcatctaga atgaccggta cacctgcatt tgatgctgta agaaccgatt
    13261 ttgattccgg aatgatacca agcaggtgca aagacaaaat ttcttgcaca tcttccagac
    13321 taagcatttc tccggactcc actcgatttg gatcatagcg agtcaacaac aaaaattctt
    13381 tgattggctc catccccagt tcagcgcgcc ttgatttgct ggctaaaatt ccaagcatgc
    13441 gatctgaatc tctaacagat gagacctcag ggttggtaac cacaaatgca tcatccgcgt
    13501 agtacatggc taagtaagcc cctttttcga taccggctgg agaatcgcaa acaatgtatt
    13561 gaaaatcttt ggctaattct tcgagcacct taccgacacc ctctatactt aaagcatctt
    13621 tatcccgagt ttgtgatgcg ggcaagatat aaagcaaatt acagttttta tcccgaatta
    13681 aagcttgact tagatttgcc tctccattga ttacattaat aaaatcataa acaactcgtc
    13741 gttcacaacc cataattaaa tcaagattgc gtaaaccaac atcgaaatca attacagcag
    13801 tcttgtgtcc tctttttgca agccccatgg caatcgctgc gctcgttgtt gttttgccaa
    13861 cgcctccttt gcctgatgtt acgacgatga ttctagccaa ttgactctcc tttgctaaaa
    13921 atgaagctat attaactctt taataatcaa tgcctgatct tgtaaataga tttgtaccgg
    13981 tttcctcggg gttatatctt ttagatcttc gctggtttta taatcccccg cgatagaaat
    14041 cagctctgct tgtaaatcag agcaaaagat tcgagcgtct ctgtttcctt gcacacctgc
    14101 taaggctcta ccccttaatg tattgtaaac atggatgttt ccttcagcca ttatttcagc
    14161 gcctgcgctg acttgcgtta aaataattaa atctcctgaa gcgtagatgc gctgacctga
    14221 acgaaccggt tgattaatca gtgtagtcgc agtacttaca acaatttctt tgatgacttc
    14281 atgtggagcg gctgatggtg atgaggtagc cgttttttgt atctcagctt gagaacttgc
    14341 gctgctttgt gacatatcta cgggaatgaa tagagaagcc gcttgaagat tttgctgatc
    14401 gttcccccct cgaattccaa ctggaaatag gccgatactg cgtatcagat taatgagctt
    14461 gatgatatca attccatgac tttgttgatt aagctcacgt acatcaagaa tgattggtga
    14521 atttctaaag aaatcctgag cttgtgatgt tttctcacga agcttttgct cgatcatcat
    14581 caaatcatta ctatggatta tcagcactag tacaaaaaaa gtgctgcttt taaactcaag
    14641 cacaggttgc atgtcgtaat ttttattaac gtctagcgac atccggttaa tcttgcaatc
    14701 atcatgaatt aaatatgtgg tgctctaata caggttggca attatacctt ccaatggtcg
    14761 ctaagggcta gtttttgcct tgtagagtct cattgaaaaa atattcctcg gttaattata
    14821 tttaatgatg aataaataag cgtaaaatca aagcgaacta gttgatttat catgctgtct
    14881 caacgaatga aaggctatga tggtaatcat tattgaagaa aattttaata agcaattatg
    14941 agaaaaatcc tgtctctaat ttgttactta ttcctactta gtgcctgtac tagtctacca
    15001 gatgttatta aaaatattcc ggttattgat gtgccttacg gtgtggtggc taaagatgcg
    15061 agtacttaca aaaataagca ggttcgttgg ggcggtgtga taattcaaac tgaaactgag
    15121 aaggaattga gttttgtgca aattcttttc tatccacttg attcttcagg gtacccacta
    15181 ttaaatcaag aaccagaggg ccgatttatt attgcaagca cggatttttt agaccctgct
    15241 atctatacta aagatagggt cattacagta gcgggtatgt taaacggcgg tgttgagcgc
    15301 tcaattggaa acaagaagat cagtattcca ttgttattga ccaaagctat ttatctatgg
    15361 cctaaagatt atcgaaaatc ttatattagc gatggaagat atccttactt ttactatcct
    15421 ggattttatg gttatccttt cttctatcct ggctactatg gcccatatcg atattggcgg
    15481 tagattcagt cgtgcagaaa ttgattcgta tcgctgggct aagttttttc ttcttactgt
    15541 taattcttgc tgctttgctt ttgctcatta ccgatagtca cccggtagtt aaaagagatg
    15601 ctgatatcaa tcacaaccac gtcgacagaa taaagcaaat ttttgataat caccgcaatc
    15661 atatttatcc tggggagtta ggcacggcaa aaattgcttc agaagatgtt gatgcggtgg
    15721 taaattactt tacctatctt ttgaccaaag gtagtactca aatcgaaatc actgaccgcc
    15781 aaatgctgtt tcgtttaagt attcctttat cgatgggcat cacaaccagt tatttaaact
    15841 tagagacaaa ttttattgaa gtagaaggtt tcccttctct tcagtcaata aaaatcagtt
    15901 attttcaact acctgttttt ctaatcacaa agatttttcc gaagctagca gaatggtcga
    15961 tttctattat tcctgaaata caattggcag ttagtgcgct acaacaagtt agaatgtctc
    16021 cttctggaat tgatatcgtc tatcgttggc aaggaggttt attcgataaa ttgaataaac
    16081 ccttcgcaat gagcctccct ctagtcaata aacaagaact cgatcgtttt tatcgatacc
    16141 actttatcct tactaatttt actgcgagta aaagtagccc gattccgtta tcgcaactat
    16201 taattccagt catgcgcctt gcttcagagc agtcaactaa aggaaatgca atagaagaaa
    16261 atcgggcagc aattttgatt acctcacttc atatattggg tatatcacta aaaacacttt
    16321 ttccagaagc aattaattgg cctaagcagc aaaaatatcg ggttacactg gatggacgcg
    16381 aagatttcgc aaagcatttt atactttctg ctgcaattac agcttacgcg gacaccaagt
    16441 tatctgatgc gatcggactt tataaagaaa ttgaagatgc tcgatcaggg agtggatttt
    16501 cttttaacga tattgctgct gatcgtgccg ggacaaaatt cggtgaaaaa gcagttgaga
    16561 attctatgtc agcactgaag atacaagaaa aaatatcgtc tgggcttgat gataaggatt
    16621 tgatgcctgc atggtcagat ttgcctgagt tcttaaccga agcagtattt aaagaacgat
    16681 tcggtgatat taatacgcct tcttatttac agataatgga caaaatcgag cagcgtattg
    16741 caaatttaat tatttttcac tagcagctat tgcattaacc gtttcatggt atcgagcaaa
    16801 gtttcgttat ctggatctgt ttcaagtgca gaacgccaaa ttttttctgc attttctctt
    16861 tctccttgaa cccacaatac ttcacctaaa tgcgctgcaa tctctggatc aggtcgagaa
    16921 ctaaatgcaa ggttaagata atttaagcct tcctggattt ttcccattcg aaagtaaacc
    16981 caacccagac tatccataat atagggatca tctggggata attctactgc tttttggatt
    17041 aacgataatg cttctgctag gcgaatgccg cgctcagcca aactatatcc taacgcatta
    17101 taggcgtgtg cattatcagg tttgagttca attaatcgcc gtaaatcttt ttcaacaatg
    17161 tcaaatcgat caatcttatc tgcagcgagt gctctgtcat agagtaattc gggttggtct
    17221 ggatttctag ccaggttttt gtctaataga ttaaacgcat cttgatgttt acctacttct
    17281 ctcagcagtt gtgcttcagc aagcattaac tgaattcgtt gctggtcagt ttcagtaggt
    17341 atttgatgga gatgctggcg tgcacggttt attccttcct ttttagcaat taaagccgcg
    17401 tatcggattt gagctgggaa aaaacgctct ccacctgaaa tgcgttggta ggtttcaagt
    17461 gcatcatcgg ttctttgcgt tacttcgtag attctagcta gattaaaata gatattgtta
    17521 ttatcttcat aaccaagctc aagcgccttt ttgaaatttt cttccgcgcc ttcaaaatca
    17581 tttaattcta gtgacaataa accaactgcc actgcaatat caggattgac agggttttca
    17641 gccatcattt tctgaaaatg ctcgcgggct aatggaaaat tcttttcagt cattagcatt
    17701 cgcacatagg cgattcgcat gtcattggct ttaggatact taccaagata gttctcataa
    17761 aaagtcatgg cttcggcatt tgcaatttgt tgcaaaatac ggccctggtg gatagcagcg
    17821 atttcccaat cagggcgtag ttctaacgca cgtttcattt ctttgagcgc tatatcaagc
    17881 tgattatttg accaagcggc ttgcgataca gcaaaatgag attcgggtaa gttgggatag
    17941 cctgctgcga gcgattgaac gagcttatag gtttctgcct tttggggatg tcgtgataat
    18001 agcttattta gatgcatgaa tgctttatcc accgagctct tttctgacga tagcaatttc
    18061 tcgagatgtg gttttgcttt gtcgagttta cccatactga caaacaatgc cgctgctgct
    18121 tgtcgggcat tcattgagtt gggttctaaa ttaacccagc gtgctacagc ttcttgggca
    18181 gcgtcaggac gacgtgcacg tagcgcaata tcagttgccc tttcagcgat tcggggatcc
    18241 tgtgtgtttt tagctaaacg agaatagctt tgtatagcaa catcaatatt ttctcgttgt
    18301 agcgctgttt cagccaataa gtaatcaaaa agtaattcgc ttgttagctc ttgcttcggt
    18361 gagtcaattt gagattggct atcttttgcc aatcttgcac tatcggcact cccaacttta
    18421 ggtaattggt tacaacctgc aagtccaata aggagtaaga atagaaacgt tttagatttc
    18481 atttaaatta aacgcataaa taataatagg actgataact aaataatcag ttctttaaac
    18541 tacgacatat ttttcaattt acatgacgca agtttataac gaacactcat gaataatcat
    18601 cttatttttg tatttttaat aaaaatcaaa aaataaatca ttgagttgag aaaggattgg
    18661 ctctgctatt atcgggttga ttttgtggaa aaggatttgc tggcccacct ctattttgtc
    18721 cggaaggggt tgattgacca gtaaatggat tggtattctg actttgtccg ggagatccgg
    18781 ggggggcatt gggttgagtt tgttgtcgtc cctgacccgt tgttccagat gcgtgattaa
    18841 acttccaatc ttggtaactc ttagcttttg agaatgctgc gtattgttca ggaaaattag
    18901 cgcgtttaat gggtatttta gttgataagc tgtaaacacc ggtaatacct ttctcaccct
    18961 gtttaataag gccccattca aagctatttg tcatgggatc catataaatt ttccttagat
    19021 accgtt
//
LOCUS       DAOQLJ010000028        18885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1016_length_18885_cov_6.199416, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000028 DAOQLJ010000000
VERSION     DAOQLJ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 18885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18885
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1016_length_18885_cov_6.199416"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PK522_03570"
     CDS             complement(<1..369)
                     /locus_tag="PK522_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNC40843.1"
                     /translation="MIKSLLRWVGWLLLLLFSLLLLLQAWFLLHILYWNQYNPSSSAF
                     MQIRWEIARQNQPHVKFHYQWVDYEKISPHLKRAIIAAEDSQFLNHAGFDFKAMQNAL
                     EKNWQEGRWASPRRRSAPAPR"
     gene            complement(448..1266)
                     /gene="aroE"
                     /locus_tag="PK522_03575"
     CDS             complement(448..1266)
                     /gene="aroE"
                     /locus_tag="PK522_03575"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041352350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNC40844.1"
                     /translation="MTDRYAVIGNPIHHSKSPLIHREFARQTQQDMSYEAILSPLDQF
                     VHTVRSFQNQGGKGLNVTLPFKIEAFRLASQLTERAQSAQAVNTLQFCNNSEILGDNT
                     DGAGLVRDIEVNQGVSIRNKNILLMGAGGAARGVILPLLKLSPNRLTIANRTLDKAIE
                     LQQQFAGFGNIRSTSYPELIQEKFDIIINATSASIANELPPLTPNVFVAQTLAYDMMY
                     GKQLTPFLQFAQCSGVNHLVDGIGMLVEQAAESFYLWRGIRPETTTVIRMLKNS"
     gene            complement(1325..2227)
                     /locus_tag="PK522_03580"
     CDS             complement(1325..2227)
                     /locus_tag="PK522_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNC40845.1"
                     /translation="MIDRTLQISSPWFEDQTNRLYVAIAISMAIHIFILMGINFKLPD
                     PPFNNIAKSLEVILVNSKSDQKPTEAKVLAQSNLDGGGNVDENQRAKTPFPVLPQSKP
                     SINLHEATQKTKQLEAEVKRLMTAAKSKPQIPQTTLAQTQTETKQPIQLDSTDLLQRS
                     LEIARLEAQIAKEHEEYQKRPKRKFIGARTREYRFARYVEDWRMKVERIGNLNYPEAA
                     KKEKLYGSLQLTVGIRSDGSLESIGIDRSSGFKILDEAAIRIVRIAAQNGFSAFPPEI
                     SRDTDILHITRTWVFARSDKLHSE"
     gene            complement(2386..4251)
                     /locus_tag="PK522_03585"
     CDS             complement(2386..4251)
                     /locus_tag="PK522_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNC40846.1"
                     /translation="MNVFYEEAGTFKVGTILAESANSLQVEATHGKRSKIKISAVLLQ
                     FDVPPVYEFMYQAQNIAKDLDINFLWECCNQNTEFTSKTLATEYFGHLPSAVEIAAIM
                     ISLNNAPIYFYKKGQGRYKAAPPEALRAALASQEKKRLDQEKQAHYMQQLNQFILPEA
                     FQKQLDALLYAPDKNTIEWKALEAAAQTTKLSPIKLLEKCGAIPSSHDYHFNQFVREY
                     FPDGVAFDVIQNDPTLDHLKNLTTASEVKAFSIDDATTTEIDDAFSVQPLSLGSFRIG
                     IHIAAPALGILPGSQLDQAAAKRLSTVYLPGKKITMLPDEVIQHFTLVESGYSPALSL
                     YLDVSDDFTVMTKQSKIEMIQVDANLRHDSLEQLFNEETLRRKILDYPFAKELELLWN
                     FAKKMEALRGKENDINNEKIDYSFLIDGERVTIQERRRGSPIDKVVSELMIYTNTEWG
                     KQLAEEGVAAIYRNQSGGKVRMSLSPAPHQGLGVSQYAWSSSPMRRYVDLINQRQLIA
                     LLTGAPPPYTKTSHELMASMHNFEQIYTIYSEFQRAMERYWCLRWLIQEKIQQIEGQI
                     IRENFVKFDHLPLVTRISTLPNLEPGTAVLVEISHIDLLERTLNARFISKINDHA"
     gene            4703..4903
                     /gene="rpmE"
                     /locus_tag="PK522_03590"
     CDS             4703..4903
                     /gene="rpmE"
                     /locus_tag="PK522_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNC40847.1"
                     /translation="MKEGIHPNYQDILVTCSCGNSFTTRSTLCKALHVEVCSACHPFY
                     TGKQKILDTAGRVEKFNRKYAK"
     gene            complement(4974..5195)
                     /locus_tag="PK522_03595"
     CDS             complement(4974..5195)
                     /locus_tag="PK522_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40848.1"
                     /translation="MENEPINRQSISTEADVDHLITLYKEVCTENTQLKKQLADVLQK
                     HSQLTDKIETAAYRLEKLLSDLPIDKKQE"
     gene            complement(5566..5642)
                     /locus_tag="PK522_03600"
     tRNA            complement(5566..5642)
                     /locus_tag="PK522_03600"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5606..5608),aa:Met,seq:cat)
     gene            5844..6311
                     /locus_tag="PK522_03605"
     CDS             5844..6311
                     /locus_tag="PK522_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNC40849.1"
                     /translation="MRLTTKGRFAVTALLDLAMQHSNNPVTLADISQRQKISLSYLEQ
                     LFAKLRQRELVDSVRGPGGGYCLAKSLDQVSIADIILAVDEPIDNRRCGGKENCLDEG
                     KCITHDLWAELNDIIFNYLGSVTLKQLIDDQKKREQQQGQSVVKLYDMREAAF"
     gene            6327..6668
                     /locus_tag="PK522_03610"
     CDS             6327..6668
                     /locus_tag="PK522_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNC40850.1"
                     /translation="MAITLTERAAKQICNQLDQQGKGLALKIGLKQSGCSGFGYTFDY
                     VDKIEESDELFITHNAKVLIPKENLSHFDGAELDFVKEGLNSSFRFNNPNVENACGCG
                     ESFGLKISEEN"
     gene            6683..8119
                     /gene="sufB"
                     /locus_tag="PK522_03615"
     CDS             6683..8119
                     /gene="sufB"
                     /locus_tag="PK522_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNC40851.1"
                     /translation="MSALLQNLVNQPYKHGFVTEIESDIAPKGLNEEIIRMISAKKNE
                     PDWLLSFRLRAYEKWLSMTEPQWQNVKYPKIDFQDISYYSAPKPKKKLASMDEVDPEL
                     LRTFEKLGVPLHERAALAGVAVDVIFDSVSVATTFKEKLAEVGIIFCSISEAVQNYPE
                     LVQKYLGSVVPVGDNFYAALNSAVFTDGSFCYIPEGVKCPMDLSTYFRINTEGSGQFE
                     RTLIIAENGASVSYLEGCTAPRFDTNQLHAAVVELVALDNAEIKYSTVQNWYAGDEKG
                     IGGIYNFVTKRGLAKGINSRISWTQVETGSAITWKYPSCILRGENSVGEFYSVAVTNN
                     YQQADTGTKMIHIGKNTRSTIVSKGISAGHSNSSYRGLVRIAPTAENARNYSQCDSML
                     IGDQCGANTFPYIQVHNNTAVVEHEASTSKIGEDQLFYFAQRGIGAEEAVSMIINGFC
                     KEVFQQLPMEFAVEATKLLSFKLEGSVG"
     gene            8116..8913
                     /gene="sufC"
                     /locus_tag="PK522_03620"
     CDS             8116..8913
                     /gene="sufC"
                     /locus_tag="PK522_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNC40852.1"
                     /translation="MIDQNSKTLLEVRDLHAKVNDVEILKGVNLTVRCGEIHAIMGLN
                     GSGKSTFAKVLAGHPGYQVTAGSVSFDNQDLLSLEPEVRSRNGIFLAFQYPIEIPGVA
                     NSQFLRLAYNTNQLSRGKEELDPLEFDDFVREKMQLLEMNVDFLDRSVNEGFSGGEKK
                     RNEILQMALLEPKLAILDETDSGLDIDALRIVASGVNKLTNKDNAIILVTHYQRLLDY
                     IVPDYVHIMESGRIIKTSGKDLAKELEVHGYDWIKADQLAKSAESSI"
     gene            8910..10226
                     /gene="sufD"
                     /locus_tag="PK522_03625"
     CDS             8910..10226
                     /gene="sufD"
                     /locus_tag="PK522_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNC40853.1"
                     /translation="MTLMTLSNNDYINSLVQRWGSYSNSTNVAWLNQLRSEALHCVNK
                     LRMPTTHMEAWRFTDLSALAKLSFPEALLFPRDQLGELARFFLSESESEYRLVFIDGH
                     YVAELSQIDSSEKIIQGNLSSLILSHNQIISSHLGKLAEFQDDLFTALNTAFIQDCAC
                     VILPRNVSIARPIHLLFISTQNETTSYPRCLVVAESGSHATIIEDYVATNENVYVTHS
                     VAEFFLADSAKINHYRIQRDSNQAFHIANHVVSLSASSYYAGVNITFGAQISRFSQRI
                     ELGGEGAECGIEGFTLITGQQLADTHTFIDHIKPQGKSRQIHKCIADGSAHGVFNGAV
                     MVRPHAQQTDSAQMSRSLLLTDKARIDAKPQLEIFADNVKCSHGATVGQLNKEEIFYL
                     RSRGISESVARNLLTYAFGGEVIEHIAVPSLKAQLEETVLLRTQIA"
     gene            10232..11494
                     /locus_tag="PK522_03630"
     CDS             10232..11494
                     /locus_tag="PK522_03630"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNC40854.1"
                     /translation="MKAADILFQADNVSATCINQFRADFPILNLKVGDKPLVYLDNAA
                     SSQMPQSVIDRLVRYQTTQHANIHRAVHHLSEIATHEYEVARQKLQHFINAREEREII
                     FTSGTTDAINLVMHGYGRKFIQKGNEIILTHLEHHSNIVPWQMLAEEKEAVIRVVPIN
                     DSGELLVEEYEKLFNENTRFVALTHVSNALGTINPIKEMIAFAHQRGVPVLIDGAQAA
                     AHLTIDVQALDCDFYTFSAHKLCGPTGIGILYGKARHLEAMQPFKGGGDMISSVTFEK
                     TTYNTLPYKFEAGTPPIAAAIGFGAAIDYLSNIGLGKIARYEHELLTYATERLSVIPG
                     VKIVGNSVSKVAVISFTMDGIHPHDVGTLLNQEGIAVRTGHHCAQPIMQRFNIPATSR
                     ASFAFYNTQDEVDALIEGIQIVKKVFLS"
     gene            11491..11973
                     /locus_tag="PK522_03635"
     CDS             11491..11973
                     /locus_tag="PK522_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNC40855.1"
                     /translation="MNSKTIYQEVILDHNRKPRNYGSLDQPSHHAVGHNPLCGDRIDL
                     GLDMINNQINQIAFQGESCAICKASASMMTTAVKGKSPAEAEILIQEFRSMVMGTLNL
                     SEQHHLGRLAVFEGVRDLPTRVKCAILPWHTLHAALHSVSNISTEANEEVAQASTVSI
                     "
     gene            12021..12581
                     /locus_tag="PK522_03640"
     CDS             12021..12581
                     /locus_tag="PK522_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNC40856.1"
                     /translation="MPKIAEIEGTPNPNALKFVLKEPLTWGVTRSFDSVEQAKDDPLA
                     SALFDIDHVTNVFYVDRWITITQDGEADWQDLARAVADPIRAAPGATSQSAATIAAAD
                     ELLANLSPEDQQRLERINILLDEEVRPFLQNDGGDLHVVGFEGNTLRIHYQGACGTCP
                     SSISGTLRGIENLLRTIEPDIELISA"
     gene            13048..14409
                     /locus_tag="PK522_03645"
     CDS             13048..14409
                     /locus_tag="PK522_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNC40857.1"
                     /translation="MKLNLKKKNQIISAAIGSMLLIGANTSMVHANTSTNPLSFLDNY
                     GIKFGGWVHGGATFNPYITDGFNGPVTFADQANRVQLNQFNLFLERAVVTEGKKWDVG
                     FRADFMFGTDAIFTQAYGNPAFDVNNGRALDDRGNWDLDICCKSLRTYGIAIPQTYAE
                     IYAPFGNGLNVKVGHFYTPIGYESVPAPNNFFYTHAYTMQYGEPFTHTGVLGNYHINK
                     NFTAMAGAIGGSGTGGWDGNFDRQMGNWGGIGGITWASDDKKTSLNISGTGSSTSTRN
                     SSFWGMFSIVFKHMITDKTHFILQHDHGFADNVLLNNLHFANVNKNAEWYGINMHMYY
                     DISRELSIGVRGEWFRDRDGFRVFSPGRVGAATNHLGNSYALGGATQLASSTPSDYYA
                     VTLGLNWKPARMMNLADTRLKGLNIRPNFRYDVADGLHGSYRPFGGNNDQVVMSLDAI
                     LPF"
     gene            complement(14500..15447)
                     /gene="rluD"
                     /locus_tag="PK522_03650"
     CDS             complement(14500..15447)
                     /gene="rluD"
                     /locus_tag="PK522_03650"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HNC40858.1"
                     /translation="MPSEAKGTSIELIVPQDVAGLRLDQVLAKLLPQWSRSRLQSWIN
                     EKHITVNGQTTTAKQKLWGNETIQIIPPIASHEQADRAEAIPLAIVYEDNDILVIDKP
                     VGLVVHPGNGNKQGTLLNALLYHSSLFANVPRAGIVHRLDKDTSGLLVIAKNIEAQTS
                     LVRQLQQRTVKREYLAVVWGEVKQEGWIDAPIGRHPIHRTRMAITAKGKPARTHYRIL
                     TPFKGYTLLQCNLDTGRTHQIRVHMHSIGHPLIGDSVYTDRTKQLPKEAGYFTHRFHR
                     QALHAYRLTLEHPSLQQPMSWESPIPKDIKELLSQLTQL"
     gene            15506..16306
                     /locus_tag="PK522_03655"
     CDS             15506..16306
                     /locus_tag="PK522_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNC40859.1"
                     /translation="MGRSLILLLTIWLSACSYFKEKDIDDSQWSPNKFYTEAKNELTN
                     QNYAAAIKLFEGLEARYPYGRYAQQAQLEVAYAHYKDQQQASAIAAADRFIQLYPHHP
                     NLDYAYYIKGLANFHDDLGILGFFTSRIIKQDMSERDSKAARESFDNFKELATRFPKS
                     KYTPDALKRMAHLVNVVARSEIYVARYYMKRKAYVAAVRRAQFILEEYPQTPSTEEAL
                     FIMAKAYDKLGLNDLREDVERVMRRNFPESEYLSDTNPLGRESWWKFW"
     gene            complement(16339..16812)
                     /locus_tag="PK522_03660"
     CDS             complement(16339..16812)
                     /locus_tag="PK522_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNC40860.1"
                     /translation="MTDYRHILLAVEFSEQDQFVTRKAERIATLFNAKLSIIHVLDNI
                     PMPDTAYGTIIPLDIDSMNELLEGEKNKLLEIGDQLGIQSDSLYLVWGKPEVEIIRIA
                     NQENADLIIVGSHGRHGLALLLGSTASGVLHHAQCDVLAIRLEDGWAGSAMLAPA"
     gene            17115..18689
                     /locus_tag="PK522_03665"
     CDS             17115..18689
                     /locus_tag="PK522_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase"
                     /protein_id="HNC40861.1"
                     /translation="MPTPSSSSRVNSINKTGDNRIDPLLFGQRWASQSISYSFPSQNS
                     AWSTDSASGYGPRNAGGEPWHPSFLPLSELDRSYFELALQQWANVANVHFNLITESSD
                     SVGDIRVAYSQTAGMADAEAWSYLPTGIAASGDIWINSTSDSAKVDWIPGDYAFLTVL
                     HEIGHSLGLEHPFDDPSFPATLDSMSTTIMSYSALPGDQNSFFDFYPTTPMPLDILAI
                     QYVYGANNQYHQEDTVYRYDDTKTYHETIWDSGGNDWITYAGGKEIAIIDLREGEGSY
                     IGNSVLAFDSAQNPDLAMNIWIAYNVVIENATGGVNDDLLIGNDHANTLIGGEGADDF
                     IGRKGNDVLQGEGGIDTAVYNGQRTQYVVNKTQEGYTLVDSSGGDGSDTMVGVERLEF
                     GDIGLALDLDGNAGLVAKLLGAVFGADAIDQADYVYAGLSLLDTGTTTDALATLALNV
                     AGIQSNDGLVTLLWQNLFGGAPTQNEKSPYVQMLDQGEITQAGLTQAAANTTINQANI
                     DLVGLVQNGIEFTLVA"
BASE COUNT         5469 a         3468 c         4171 g         5777 t
ORIGIN      
        1 acgtggtgcg ggcgctgaac gccgtcgggg cgacgcccac cgaccttctt gccagttttt
       61 ttctaaggcg ttttgcattg ccttgaagtc aaaacccgca tgattcaaaa attgactatc
      121 ttcggccgca ataattgcgc gcttgagatg gggagaaatt ttttcataat caacccattg
      181 gtaatgaaat ttcacatggg gttggttttg gcgtgctatt tcccatcgaa tctgcataaa
      241 agcgctggac gaaggattat actggttcca gtagaggatg tgcagcagaa accacgcttg
      301 gagcaataat aaaagactaa aaagtaggag taacaaccat cctacccatc gcagcaatga
      361 tttgatcatt tagttacggc ggtatttggt ggatcagcga ctataggtag gtagataacc
      421 gaatcagttt tatgagatcg atacgtatta ggaattttta agcatacgta tcactgttgt
      481 tgtttcaggt cttatgcctc gccataaata aaacgattct gcagcttgct caacgagcat
      541 tccaattcca tcaactaagt ggttgacacc actacactgt gcaaattgca gaaatggggt
      601 taattgtttt ccatacatca tgtcgtaagc taaggtttgt gctacaaaaa cattgggtgt
      661 taacggaggt agctcattgg cgatactggc tgatgttgca ttgatgataa tatcaaattt
      721 ttcttgaatc agttctgggt aggaagtcga tcgaatatta ccaaagccag caaactgctg
      781 ttgcagctca attgctttat caagggttcg gtttgcaatg gtgagtcgat tgggactaag
      841 cttgaggagt ggtaggataa caccccgagc tgctcctcct gcccccatta acaaaatatt
      901 tttgttccta atcgaaacac cttggttgac ttcgatatca cgtactaacc cagcaccatc
      961 ggtgttatcg ccaagaattt cactattatt acaaaattga agggtattaa cagcttgagc
     1021 ggattgagcc cgctcagtga gttgtgatgc tagcctaaaa gcctctattt taaagggtaa
     1081 tgtgacattg agtcctttgc caccttgatt ttggaatgat cgaacagtat gcacaaattg
     1141 atccaacggg gaaaggatcg cttcgtagct catatcttgt tgcgtttgtc gggcaaactc
     1201 gcgatgaata agtggagatt tactatggtg gatggggttg ccaattacag catatcgatc
     1261 agtcatggtc taagtagaga ctcaaagtaa tatcgattgg ctaattggtt accattgcag
     1321 aaatttactc actatgtaac ttatcggaac gcgcaaacac ccaggtacgg gttatatgca
     1381 ggatatcggt atcacggctg atctctggtg gaaaagctga aaaaccattt tgtgccgcga
     1441 tccggacaat tcgaattgct gcttcatcaa gaatcttgaa acccgatgag cgatcgattc
     1501 caatggattc aagacttccg tcagagcgaa taccgacggt taactgtaaa cttccataaa
     1561 gcttctcttt tttggcagcc tctgggtaat ttagatttcc gatacgctcg actttcatac
     1621 gccaatcttc aacgtaacgt gcaaatcggt attcgcgggt acgcgcgcca atgaatttgc
     1681 gcttaggccg cttttggtat tcttcatgct ctttcgcgat ttgagcttcc aggcgtgcaa
     1741 tttcaaggct gcgctgtagt aaatcggtac tatccagctg tattggttgc tttgtttcag
     1801 tttgagtttg cgcgagtgtg gtttggggaa tttgtggttt actctttgca gcagtcatga
     1861 gtcgtttaac ttctgcttct agctgctttg ttttctgagt tgcttcatga agattaatag
     1921 atggttttga ttggggaaga actggaaacg gtgttttggc gcgttgattt tcatcaacat
     1981 tgcctccccc atctaaattg ctttgtgcga gcacttttgc ttcagttggt ttttgatcag
     2041 acttgctgtt gacaagaatg acttccaatg atttggcaat attattgaaa ggaggatcag
     2101 gtagcttaaa attaataccc attaaaataa agatatgtat ggccatcgaa atcgcaatgg
     2161 cgacatacaa gcggtttgtt tgatcctcaa accaaggact cgatatctga agtgtgcgat
     2221 ctatcattat caaataaaca gggggggtaa atataacctt tgaatcgtac taatattgct
     2281 tagttagtgc atatgctatt ttgaccgtga gctccagcat gatacttgaa aatatttagt
     2341 gcagatagac gaaatctata ccattttttt gctatttaca aaatactaag catgatcgtt
     2401 tatcttactg ataaatcgtg catttaacgt gcgttccagt aggtcgatat gtgaaatttc
     2461 aaccagcact gctgtaccag gctctagatt ggggagtgtc gatattcggg tgactaatgg
     2521 caaatgatcg aatttgacaa aattttctct aataatctgg ccttcaattt gctggatttt
     2581 ttcttgaatc aaccaacgaa ggcaccagta gcgttccatt gcgcgttgaa attcactata
     2641 aatcgtataa atttgttcaa aattatgcat ggaggccatc agttcatggc tcgttttggt
     2701 ataaggagga ggggctccgg tcagtaaggc gatgagttgt cgctgattaa ttagatcaac
     2761 atagcgtcgc atgggtgagc tactccacgc gtattgtgaa acacctaagc cttgatgagg
     2821 ggctggagaa aggctcattc tgacttttcc accgctttga ttgcgataaa ttgcggcaac
     2881 accttcttcg gcgagttgct taccccattc ggtgttagta taaatcatca attcagaaac
     2941 aactttatcg ataggagaac cacggcgtct ttcttggatt gtgactcgtt cgccgtcgat
     3001 caagaagcta taatcaattt tttcattgtt aatatcattt tccttgcccc gcagcgcttc
     3061 cattttttta gcaaaattcc agagtaactc gagttcttta gcaaaaggat aatcaaggat
     3121 ttttcgtctt agtgtttctt cattgaatag ctgttctaaa gaatcgtgac gaagattcgc
     3181 atcaacctga atcatttcta ttttgctttg cttggtcatc acggtgaaat catcagaaac
     3241 atcgagatat agcgataatg cagggctata acctgattct accaaggtaa aatgttgaat
     3301 cacctcatca ggcagcatag tgattttctt ccctggcaaa taaaccgtag aaagccgctt
     3361 tgcagcagct tgatcgagct gagatccagg taatattcct aatgcagggg ctgctatgtg
     3421 gataccaatt ctaaaacttc ccagcgacag gggttgaact gagaatgcat cgtcaatttc
     3481 tgtggtcgtg gcatcatcaa tgctaaatgc tttgacttca ctcgcagttg taagattttt
     3541 taggtgatca agcgtaggat cgttttgtat tacatcgaat gcaacgccat ctggaaaata
     3601 ttcacgtaca aattgattaa aatgataatc atgagaagaa gggattgctc cacatttttc
     3661 cagcaatttg atggggctga gtttagtagt ttgggctgca gcttcgagcg ctttccattc
     3721 aattgtattt ttatcgggcg catagagcag ggcatctaac tgcttctgga atgcttcagg
     3781 aaggatgaat tgattaagct gctgcatgta atgcgcttgc ttttcttgat ctaagcgttt
     3841 tttttcctga ctggctagcg cggctctaag cgcttccgga ggtgctgctt tatatcgccc
     3901 ctgtcctttt ttgtagaaat aaatcggtgc attatttaat gagatcataa tggcagcaat
     3961 ttctactgca ctagggagat ggccaaaata ttcggtggca agggttttgc tcgtgaattc
     4021 tgtgttttga ttacaacatt cccaaaggaa atttatgtcc agatctttgg caatattctg
     4081 cgcttgatac atgaattcgt acacaggtgg cacatcaaat tgaagcaaaa cagcggaaat
     4141 tttaattttt gagcgtttac cgtgagttgc ttcaacttgt aaagaattag cgctttcagc
     4201 tagaatcgtg ccaactttaa acgtacctgc ttcttcataa aacacattca ctgatgggac
     4261 ctttcagaaa agcgataaaa aaattgaaaa caagtcaatc atagcctaat gttgcgatat
     4321 ttttctgaaa gttttgataa agtcttttca atattttatt aatgcagaaa tgcagaatga
     4381 tttatcactg gtacgcaagc ggtctaagct ttcttgagaa aaaactatat tgatgttctg
     4441 ttgccatcaa tttatagggg gggggtgcaa aattcgatag gttgctgatt ttaataagta
     4501 gagagcgagt gattttcgcc acaactgcgt aacgatttaa atatacataa tgcgtctcaa
     4561 agtggatagt tttttgctac aaatcgaata tttctaattt gttgctaaca gttatcgatt
     4621 aaaggataat agtgtgctgc caaaatttga ataacaaatg aagtctataa tagcaagtga
     4681 gtatttaagg acataaaata ttatgaaaga agggattcat ccaaattatc aagatatact
     4741 agtgacatgt agttgtggca attcatttac tacgcgttct acattgtgta aagcattgca
     4801 tgttgaagta tgctcagcct gccacccttt ttatactgga aaacagaaaa ttcttgatac
     4861 tgcgggaagg gtagagaaat ttaatcgtaa gtatgcaaaa tagattgtgt taataagcgg
     4921 tagttagtgt ttttaagtat aatagctcga ttatgttttc ttctttgtga catctactct
     4981 tgttttttat caataggtaa atcactcaaa agtttttcta aacgataagc agcagtttcg
     5041 attttatcgg ttagctggga gtgcttctgt aaaacatctg ctaattgttt ttttagttga
     5101 gtattttctg tacagacttc cttgtacagt gtaattaggt gatcaacgtc agcttcagtt
     5161 gatattgatt gcctattgat tggctcattt tccattggga agaaatataa ttttagaata
     5221 gtctctggtc aattaactaa ccgttatagc cattccctca atttactggt tagaccccaa
     5281 gaaatctttg cgcgatacac aacgcatctc ctgatttaag gacattgaat attaataaga
     5341 tattcaattt taccatagcg ctaacaatat taataacaga gagaatcttt agtcaaatac
     5401 ttaagctatg cgatgactaa ttattagttg aatttttctg aagttaaagc tttttcgaat
     5461 gattctactt aagaaagtct tgaatagaac ggagctgtaa gctggctgca tagataagcc
     5521 taacttgtta tttttataac gtaatttttt atcaagaggg caaaatggtt gcgggggcag
     5581 gatttgaacc tacgaccttc gggttatgag cccgacgagc tgccagactg ctccaccccg
     5641 cgccagaaag tgcgatcata gcagtagatg atattctcag tcaaattgga aaagtagaaa
     5701 ggggttttat tttttaaatt tgggcagatt agagacatcg tcacgataaa tttttattag
     5761 ttatagttga ctatcttagt aaggtattat atagttgaca aaaataatca agtaatttac
     5821 caacgatgct ataaggatct tctatgagat taacgactaa aggacgattc gcggtaacag
     5881 cgctacttga tctagcaatg cagcactcca ataacccagt gacacttgca gatataagtc
     5941 agcgccaaaa aatttccctt tcttatttgg aacaattatt tgccaaatta cgtcaacgtg
     6001 aattggttga tagtgttcgt ggaccaggag gtgggtattg tttagctaaa agcctggatc
     6061 aggtttcgat tgcagacatt attttggcag ttgatgaacc gattgataat cgtcggtgtg
     6121 gagggaaaga aaactgctta gatgaaggaa aatgtataac acatgactta tgggcagagc
     6181 tcaacgatat tatttttaat tatcttggta gcgtcaccct caagcaattg attgacgatc
     6241 aaaagaaacg tgagcaacaa caaggtcagt cagtagtgaa attatatgat atgcgtgaag
     6301 ctgctttcta attattttta tttactatgg cgattacatt aaccgagcgt gcggcaaagc
     6361 agatctgtaa tcaacttgat caacaaggta aaggtttggc attaaaaata ggccttaagc
     6421 aatctggttg ttcaggattt ggttatacct ttgattatgt tgataagatt gaagaaagtg
     6481 atgagctatt tattacgcat aatgcaaaag ttctgatacc aaaagaaaac ttgtctcact
     6541 ttgacggggc tgaactcgat tttgtaaaag aagggttaaa tagtagcttc agattcaata
     6601 atcctaatgt tgaaaatgcc tgtggctgtg gggaaagttt tggattaaaa atttctgaag
     6661 aaaattaagg aaacgattaa aaatgagtgc tttactacaa aatttagtaa atcaacctta
     6721 taaacacggt tttgttactg aaattgaatc agatattgct ccgaaagggc ttaatgaaga
     6781 aatcattcgc atgatttcag ccaagaaaaa tgagcctgac tggttgctta gttttcgact
     6841 aagagcttat gagaaatggc tgagtatgac tgaaccacag tggcaaaatg ttaagtatcc
     6901 gaagattgat tttcaggata tcagctacta ttcagcgcca aaacctaaga aaaagctagc
     6961 gagtatggat gaagtcgatc cagaattact ccgtactttt gagaaattag gagtgccatt
     7021 acatgagcgt gctgcattgg ctggtgtggc tgtcgatgtg atttttgaca gtgtttcagt
     7081 tgcaacgacg tttaaggaaa aattggcaga agttgggata attttctgtt cgatttcaga
     7141 agcagttcag aattatcctg aactagttca gaaatatctt ggtagtgttg ttccagtagg
     7201 agataacttt tatgcagcac ttaactccgc tgtatttact gatggttcat tttgttatat
     7261 tccggaaggc gttaaatgtc ccatggatct ttcgacctat tttcggatta atactgaagg
     7321 ttcaggtcaa tttgaacgaa cacttattat tgctgaaaat ggcgcatcag tatcgtacct
     7381 agaaggttgt actgcgccac gctttgatac gaatcaattg catgctgcgg tcgttgagtt
     7441 ggttgcactg gataatgcgg aaatcaaata ttctacagtt cagaattggt atgcgggaga
     7501 tgaaaaaggg attggcggga tctataactt tgtgactaag cgtggtttgg ctaagggaat
     7561 taattcccgt atttcttgga ctcaagtcga aacaggctca gcaattacct ggaaataccc
     7621 atcgtgtatt ttgcgtggtg agaattcagt gggtgaattt tattcggttg cggtaaccaa
     7681 taactatcaa caagctgata ctggcactaa aatgattcat attggaaaaa atacgcgaag
     7741 cacgatcgtg agtaaaggaa tttccgccgg tcactcgaat agtagctatc gtgggttggt
     7801 aagaatagcg ccaactgcag aaaatgcacg taattattca caatgtgact caatgctcat
     7861 tggtgaccag tgcggtgcaa atacctttcc ttatatccaa gtgcataaca atactgcagt
     7921 tgtggagcat gaagcttcta cttctaaaat tggagaggat cagctgtttt actttgcaca
     7981 gcgtggaatt ggagctgagg aagctgtttc tatgattatt aatggtttct gtaaagaagt
     8041 atttcaacag ttaccgatgg aatttgctgt ggaagcaact aagttactta gcttcaaact
     8101 tgagggaagt gtaggatgat tgatcaaaat agtaaaaccc tgcttgaggt tcgtgatctt
     8161 catgcaaaag taaatgatgt tgaaatacta aagggagtaa atctcacggt aagatgcgga
     8221 gagattcacg ccattatggg gttaaatggt tctggtaaga gtacttttgc caaagtttta
     8281 gctggacacc caggttatca agttaccgcg ggatcagttt cctttgataa ccaagattta
     8341 ctttcacttg aaccagaagt tcgctctcgg aatggtatct ttcttgcttt tcagtatcct
     8401 atcgagatac cgggtgttgc aaatagtcaa tttttaagac tcgcttataa cacgaatcaa
     8461 ttaagtcgtg gtaaagagga attagatccg ctcgagtttg atgattttgt tcgagaaaaa
     8521 atgcagctat tggaaatgaa tgttgatttt cttgatcgta gcgttaatga gggtttctct
     8581 ggcggagaaa agaaacgcaa tgaaatattg caaatggcac tattggaacc gaaacttgcc
     8641 atattggatg aaacagactc aggtctcgat atagatgcat tgagaatcgt agcttcagga
     8701 gtgaataagc tgactaataa agataatgcg attattcttg taacccatta tcaacgtttg
     8761 cttgattata tcgtgcccga ttatgtgcat attatggaat cgggtcgtat tatcaaaacc
     8821 agtggaaaag acttagcaaa agaacttgaa gtgcacggat atgattggat aaaggccgat
     8881 caacttgcaa aatcggccga gtcaagcata tgactttaat gactttatca aataatgact
     8941 acattaatag tctcgttcaa cgctggggat cttactctaa ttctacgaat gtagcgtggt
     9001 taaatcaact tcgctctgag gcgctgcatt gtgttaataa attaagaatg ccaacaacgc
     9061 atatggaagc ttggcgtttt accgatctgt ctgcgttggc taagctatct ttccctgaag
     9121 cattactttt tccaagagac cagcttggtg aattagcgcg tttctttttg agtgagagtg
     9181 aatcagaata tcgcttggta tttatcgatg ggcattatgt ggcagagtta tctcaaattg
     9241 attctagtga gaagattatt caagggaatt tatcttcatt gattctttcg cataatcaaa
     9301 ttatttcttc tcaccttgga aagctagccg agtttcagga tgatcttttc acagcattga
     9361 atactgcctt tatacaggat tgtgcttgtg ttattttgcc tcggaacgta tcaattgcta
     9421 gaccaatcca tttgttgttt atttcaactc aaaatgaaac aacgagttat ccgcgttgtt
     9481 tggtcgtagc tgaatcagga agtcatgcga cgattattga ggattatgtt gccacgaatg
     9541 aaaacgttta tgttacacat tcagttgcag aattttttct agctgattct gccaaaatca
     9601 atcattatcg gattcaacgt gacagtaatc aggcgttcca cattgctaat catgtggtta
     9661 gtctatcagc gtcaagttat tacgctgggg ttaatattac atttggcgct caaatttcac
     9721 gttttagcca gagaattgaa ttggggggag agggtgctga gtgtggcata gagggcttta
     9781 cgctgattac agggcagcaa cttgctgata ctcatacttt tatcgatcat ataaaacctc
     9841 aaggtaaaag tcggcaaatt cataaatgca tcgctgatgg ttcagcccat ggggtattta
     9901 atggggcagt catggttcgt ccccatgcgc agcaaaccga ttctgctcaa atgagtagaa
     9961 gtttattgct gacagataaa gcccgtattg atgctaaacc ccagcttgaa atttttgctg
    10021 ataatgtcaa atgttctcat ggcgctacag taggtcaatt gaataaggaa gagatatttt
    10081 atttaagaag cagaggcata tcagaatcag ttgcacgcaa tttactgact tatgcctttg
    10141 gtggagaagt aattgaacat atcgctgtac cttcacttaa agcacagctt gaagaaactg
    10201 tacttttaag aacgcaaatc gcttaatact aatgaaagcc gcagatatcc tatttcaggc
    10261 tgataatgtt tcagcaacgt gtataaatca atttcgcgca gattttccaa ttctgaacct
    10321 gaaagttggc gataagccac tggtttactt ggataatgcg gcttctagtc agatgcccca
    10381 gtctgttatt gatcgtctcg tacgttatca aacgacccaa catgcgaaca ttcatcgggc
    10441 agttcatcat ctttccgaaa ttgctaccca tgaatatgaa gttgcacgtc aaaaattgca
    10501 acattttatc aatgcacgcg aagaaagaga gatcattttt accagtggaa ctaccgatgc
    10561 tattaattta gtgatgcatg gttatgggcg gaagtttatt cagaaaggaa atgaaattat
    10621 tcttacccac cttgagcatc actccaatat tgtgccctgg caaatgctag ccgaagaaaa
    10681 agaagctgtc attcgagtag tacccatcaa tgattctggg gaattattag tggaagaata
    10741 tgagaagcta tttaatgaaa atactcgctt tgtcgcactg acgcatgttt ctaatgcact
    10801 tggtacgatt aatccgatta aagagatgat agcttttgcc catcagcggg gagtacctgt
    10861 attaattgat ggtgcccagg ctgcagcgca tttaaccatt gatgttcagg cgcttgattg
    10921 tgatttctat actttctcag cacataaact ttgtggccca accggaattg gtattcttta
    10981 tgggaaagcg cgacaccttg aagcaatgca accatttaag ggtggagggg atatgatttc
    11041 ttccgtgaca tttgaaaaaa cgacttacaa tactttaccc tataagtttg aagcaggcac
    11101 tccgccgatt gcagcagcga taggatttgg cgctgcaatc gattatttat caaacattgg
    11161 tctaggtaag atagcgcgct atgagcacga gcttctaact tatgcaacag agcggctgag
    11221 tgttattccc ggtgtgaaga ttgttggcaa ttcggtttca aaagttgctg ttatttcttt
    11281 taccatggat ggaattcatc ctcatgatgt gggtacgttg ctcaatcaag aaggtattgc
    11341 tgttcgtaca gggcaccact gtgctcaacc aattatgcaa cgattcaata ttccggctac
    11401 ttcgcgtgct tcatttgctt tctacaatac ccaagatgag gttgatgctt taatcgaagg
    11461 gattcaaatt gttaaaaagg tttttttgtc atgaattcaa aaacaattta tcaagaagtc
    11521 attttggatc ataaccgtaa gccacgaaat tatggaagct tagatcagcc aagtcatcac
    11581 gcagttggac acaatccttt atgtggcgat cgaattgacc ttggtcttga catgatcaac
    11641 aatcaaatta atcaaatagc gttccaaggt gagtcgtgcg caatttgtaa agcttctgca
    11701 tccatgatga caactgcagt taaagggaaa tcacctgcag aagctgaaat tctcattcaa
    11761 gaatttcgga gtatggtaat gggaaccctg aatttgtctg agcaacatca cctcggaaga
    11821 ttagctgtgt ttgaaggtgt tcgtgacctg cccacaaggg taaagtgtgc cattttacct
    11881 tggcatacct tgcatgctgc ccttcactct gtctccaata tctcaactga agcaaatgaa
    11941 gaggtagcgc aagcttcaac ggtatctatc tagaatttta gttaataaca cattattttt
    12001 tgcagaacat taaaagcaga atgcctaaaa ttgctgaaat tgaagggaca cctaatccca
    12061 atgcacttaa atttgtgctg aaagaaccgc tcacctgggg tgtaactcgt tcttttgata
    12121 gtgttgaaca agcaaaagac gatccgcttg cttctgcatt atttgatatt gatcatgtca
    12181 ccaatgtctt ttatgttgat cgctggataa caattactca agatggggag gctgactggc
    12241 aagatttagc ccgtgccgtt gcggatccta ttcgagcggc cccaggggca acatctcaaa
    12301 gtgcagcaac gattgcagca gcagatgagc tacttgcaaa tcttagcccg gaagatcagc
    12361 aacggctcga acgtatcaat attctattgg atgaagaggt acgtcccttt ttgcagaatg
    12421 atggaggcga tctccacgtc gttggttttg aagggaatac gctgagaatt cattatcaag
    12481 gtgcgtgtgg aacctgtcct agttcaattt caggtacatt aagaggaatt gaaaatttat
    12541 tgagaaccat tgaacctgat atcgaattga tttctgctta agcgatttgg tcaatttatc
    12601 ctaagtattt ggaagcaata ttttttgtta tagtttagcg cgaagctttg cttatccaga
    12661 attacattct ggataagcaa attgccagat catcccccga tgctttcgtt tcagaaaaac
    12721 aacgaactcg ttgaatccct ttgcagcccc ctaatatgaa atttctctac tattgatcaa
    12781 tgttggtata cgttctttat tacgagtatc ccaaggtttc tagaaaatta atacccattt
    12841 cttagagagc tatcagtatt actttgcaac agtataatat ttttgatcaa taagtgttgt
    12901 tagtttcata cacttgtaaa gattttatga aaattgttca ttgacctaaa tttgtgttgt
    12961 gtgtaaagtc aaaccctcag acgaagtaag gttattaagt tttatattga aaatactgaa
    13021 aaattctaaa agaaagaaga gggaagaatg aaacttaatc taaaaaagaa gaatcagatt
    13081 atttccgcag caatcggtag catgctactc attggtgcta ataccagtat ggttcatgca
    13141 aatacatcaa ccaacccact gagtttctta gacaactatg ggattaaatt tgggggatgg
    13201 gttcatggtg gcgcgacttt taatccctat attacggatg gttttaatgg tccagttact
    13261 ttcgcagatc aagcgaaccg ggttcaatta aatcagttta atctattctt agaacgcgca
    13321 gttgttacgg aaggtaaaaa atgggatgtt ggcttccgag cagacttcat gtttggtaca
    13381 gatgcgatct ttacccaagc ctatggtaat cctgcattcg atgttaataa tggtagagcg
    13441 ctggatgata gaggaaactg ggatctcgat atctgttgta aatcactacg gacctatggc
    13501 attgcaattc cacaaacgta tgctgaaatt tatgcgccgt ttggcaatgg tttgaatgtc
    13561 aaagtagggc atttctatac accgatcggg tacgaatctg ttcctgctcc caataatttc
    13621 ttttataccc atgcttatac gatgcagtat ggtgagccct tcactcatac cggtgtttta
    13681 ggtaattatc acatcaataa aaattttacg gcaatggctg gtgcgattgg tggtagtgga
    13741 accggtggtt gggatggtaa ttttgatcgg caaatgggga actggggtgg aattggtggt
    13801 attacttggg caagcgatga taaaaaaaca tcactgaata ttagtggtac gggtagttca
    13861 acttcaaccc gcaacagtag cttctggggt atgtttagta tcgtgttcaa gcatatgatc
    13921 acggacaaaa cccattttat cttgcaacat gatcatggtt ttgcagacaa cgtcttgtta
    13981 aataatctcc actttgcgaa tgtgaataag aatgccgagt ggtatggtat taacatgcat
    14041 atgtattatg atatttctcg tgaattatcg attggtgtga gaggcgaatg gtttagagac
    14101 cgtgatggtt tccgggtatt ttcacccggc cgtgtcggtg cagcaacaaa tcacctcggg
    14161 aacagttatg cattgggtgg tgcaactcag ctagcaagta gcacaccgtc tgattactac
    14221 gcagtgacac tcggtttaaa ttggaaacct gctcgaatga tgaatcttgc agatacccgc
    14281 ctaaaaggtc ttaatattcg tccaaatttc cgctatgacg tggctgatgg acttcatgga
    14341 tcttatcgac cctttggcgg aaataatgat caagtcgtta tgtcgctgga tgccatatta
    14401 cctttctaaa ttagtaacca aaatgtaata aactgaaatg cacctgcggg tgcatttctt
    14461 ttatgatcct tctatttaaa tctcacgcaa tatagtagat cagagctggg taagttgact
    14521 gagcaattct ttgatatctt ttggaatagg ggattcccag ctcattggtt gctggaggga
    14581 agggtgctcc agggttaagc gataagcgtg caatgcttga cgatgaaaac gatgagtgaa
    14641 gtacccagct tctttgggaa gttgtttggt acgatctgta taaactgaat caccgataag
    14701 tggatggcct atgctatgca tgtgaacccg aatttgatgc gtgcgtcccg tatccagatt
    14761 gcactgtagc aatgtatatc ctttaaacgg ggttaatatc cgataatgtg tgcgagcagg
    14821 ttttcccttt gcagtaatcg ccattcgcgt tcgatgaatg ggatgtctac caataggcgc
    14881 atcgatccaa ccttcttgct taacctcgcc ccatacgacg gctaaatact cgcgtttcac
    14941 ggtgcgttgc tgtaactgac gaacgagact ggtttgagct tcaatatttt ttgcaatgac
    15001 cagcaagccg ctggtatctt tatcgagacg atgtacgata cctgctctcg gtacatttgc
    15061 aaacagactg gaatgataca acagggcgtt aagcaaggtt ccttgcttat taccgttacc
    15121 cgggtggacg actaaaccaa caggtttgtc gatgactaga atatcattat cttcataaac
    15181 aatggctaat gggattgctt cagctcgatc ggcttgctca tgagaagcga ttggagggat
    15241 gatctggatt gtttcattac cccaaagttt ttgtttagca gttgtagttt gaccatttac
    15301 cgtaatatgt ttctcattaa tccaggactg taagcgactg cgagaccatt gtggcagcag
    15361 ctttgcgagt acctggtcta atcgaagtcc tgcaacatct tgtgggacaa ttaactcaat
    15421 tgatgttcct ttagcttcag agggcatggt gagaacagac tttacgttat aatttgaaga
    15481 tattattgca tcgataggat ctgttatggg gcgtagttta attttgttac taactatttg
    15541 gctatcagcc tgtagttatt tcaaagaaaa ggatatagat gatagtcaat ggtctcccaa
    15601 taaattttat acagaagcta agaacgaact taccaaccaa aattatgcgg ctgcgatcaa
    15661 attatttgag gggctagaag cacgctatcc ctatggacgc tatgcacaac aagcacagtt
    15721 agaagttgcc tatgcacatt ataaggatca acaacaagcc tcggccattg cggcggctga
    15781 tcgattcatt caactttatc cgcaccatcc aaacctagat tacgcctatt acattaaagg
    15841 gcttgctaat tttcatgatg acttggggat tcttggtttc tttacatccc gtattattaa
    15901 gcaagatatg agcgagcgag attctaaggc tgcccgagaa tcatttgata atttcaagga
    15961 attagctact cggtttccta aaagtaagta tacgccagat gcactcaaac gcatggccca
    16021 tcttgtcaat gtggttgcaa gaagcgaaat ttatgtagcg cgatattaca tgaagcgtaa
    16081 ggcttatgtg gctgcagtgc ggcgcgcgca gtttattttg gaagaatacc cacaaacccc
    16141 ttcgacagaa gaagcgctat tcatcatggc taaagcttat gataagttag gattgaatga
    16201 tttaagggaa gacgttgaac gtgtaatgcg ccgaaatttc ccagaaagcg agtacctttc
    16261 cgacactaac ccgcttggaa gggaatcttg gtggaaattt tggtagtgcg ctcgatttag
    16321 aattgttttt ttcttttact aagcgggcgc caacatcgca gaacctgccc aaccatcctc
    16381 gagacgaatc gctagcacat cgcattgtgc atgatgaaga acaccactgg cggttgagcc
    16441 tagtaataaa gccaaaccat gtcgcccatg tgaaccaacg atgatgagat cagcgttttc
    16501 ttggtttgca attcgtataa tttcaacctc aggctttccc caaactaaat acaatgaatc
    16561 ggactgtata cctagctgat cgccaatttc taacagttta ttcttttcgc cttctagtaa
    16621 ttcattcata gaatcgatat cgagtggaat gatggttcca taagcggtat ccggcatcgg
    16681 gatattatct agcacatgga tgatgcttag cttcgcatta aaaagagtgg ctatacgctc
    16741 agcttttcgt gtaacgaatt gatcttgctc tgagaattca acagcaagca gtatatggcg
    16801 gtaatctgtc attgtttatt tccttaaagt gtatgaacgc gatcaatatt ttgtttattt
    16861 tacgcgatct accttatgtt agaaaatctg aaataaaatt gagtggtagc gcatgcagga
    16921 aaatagatgg atagatgcga tggaatttaa actggatgta caatttatgg tgagaaaaag
    16981 ccactgaatc aaaattaaac ggacaacgat accttatttg ggtatttaac cggttagcat
    17041 ccatctagaa tggtatcaaa cgtactacgt gctaatttga ttttattgtt tatgattgtt
    17101 taggaaatta tctaatgccc acaccgtcca gctctagccg tgtaaattcg attaataaaa
    17161 caggagataa ccgcattgac ccgctattgt ttgggcagcg ttgggcaagt cagtcgattt
    17221 cctacagctt tccatcacaa aactctgcgt ggtcaaccga ttcagcctca ggttatgggc
    17281 cacgcaatgc cggtggtgag ccttggcatc catcttttct gccattaagt gaattagatc
    17341 gatcttattt tgaattggcg ctgcagcaat gggcaaatgt cgctaatgtt cattttaatc
    17401 ttataactga atccagcgat tcagtaggcg atattcgggt tgcttacagt caaacagcag
    17461 gcatggcgga tgccgaagca tggtcctatt tgccaacagg gatagccgcc tctggagata
    17521 tttggattaa cagcaccagc gatagcgcta aagtggattg gataccgggt gattatgctt
    17581 ttttgaccgt actccatgaa attggccatt cgttaggttt ggagcatcct tttgatgatc
    17641 ctagttttcc agcgacgcta gatagcatgt ctacgacaat aatgagttac tcagccttac
    17701 caggagatca aaattcattc tttgatttct atccgacaac cccgatgccc ctcgatatct
    17761 tagccattca atatgtctat ggcgcaaata atcaatatca tcaggaagat acggtttacc
    17821 gctacgatga tactaaaact taccatgaaa ctatttggga tagcggtggg aatgactgga
    17881 ttacctatgc ggggggtaaa gaaattgcga ttatcgatct ccgagaaggt gaaggatctt
    17941 atattggtaa cagcgtacta gcattcgata gtgctcaaaa tcctgactta gcgatgaata
    18001 tctggattgc ctataacgta gtgattgaga atgcaaccgg tggtgttaac gatgacttat
    18061 tgattggtaa tgatcatgct aatacgttaa ttggtgggga aggtgctgat gatttcattg
    18121 gccggaaagg taatgatgtg ttacaggggg aaggcggcat tgatactgcg gtatacaatg
    18181 gtcagcgcac tcaatatgta gtcaataaaa cacaggaagg ttatacactc gttgattcat
    18241 caggaggcga tgggtcagat acgatggtgg gagttgaacg tctagaattt ggagatattg
    18301 gtttagcact ggatcttgat ggaaatgcag ggcttgtcgc taaattattg ggggcagtat
    18361 ttggtgcaga tgccattgat caggctgatt atgtgtatgc aggattgtct ttactcgata
    18421 ccggaactac taccgatgca ctggccacgt tagcattaaa tgttgcgggt attcaaagta
    18481 acgatgggct agtgacttta ttatggcaaa acctatttgg tggtgctccc acacagaatg
    18541 aaaaatcacc ttatgttcag atgctcgatc aaggtgaaat tacacaggca ggattaaccc
    18601 aggctgcggc gaatacgacg attaaccaag ccaatatcga tctggtaggt ttagtgcaaa
    18661 atggaattga atttacttta gttgcttgat gacctattaa caggccatag aacataatat
    18721 gtccgctcaa aggggacaac tccatattcc tacccgcaca atagcgggta gatgggttga
    18781 atcagtagcg tcgataatgt acagctttag aaagcgtgaa tgaaaaaagc tacatttttt
    18841 ggcttgttcg gtttacttca aaaaatattt ataagtgtga taagt
//
LOCUS       DAOQLJ010000029        18207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1072_length_18207_cov_6.242948, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000029 DAOQLJ010000000
VERSION     DAOQLJ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 18207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18207
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1072_length_18207_cov_6.242948"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1949
                     /locus_tag="PK522_03670"
     CDS             <1..1949
                     /locus_tag="PK522_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNC40862.1"
                     /translation="CTRCVRFGQEIAGIMELGMSGRGEHSEILSFVGKTVDSELSGNV
                     IDLCPVGALVSKPFRYSARTWELSRRKSISPHCGLGSNLIVQVKQNRVMRILPRENEE
                     INECWLSDKDRFSYEGLNSEDRLSKPMIKRNNQWITCDWQEALLFVVQNLKACVSQYG
                     GRAIGALGSPHSTLEELYLLQKLVRGLGSNNIDHRLRQSDFRADNYQQGIPWLGMPIA
                     EVSNLKSILIIGSNLRKDHPLLALRVRQAVKSGAQLSIVNSTDDDLLTKVAQRAIVAP
                     SELVRTLAQILKAIIEYRYVEIADKIKETLTKVNVTDVTKAIANNLVSNQSAGIFLGN
                     LSQHHPNYSEIFLLAQEIAKVTSAKLGVFGEAANSVGAYVAGAIPNRKLGNLTTNGEE
                     VGLNAIQMLNAEAVSACKAYILVNLEPEYDTYDPQSALERIKGAEFVVSLSSYKGNIT
                     DYADVILPIAPFTETSGTFINTEGRVQSFNGVVSPLAEARPAWKVLRVLANLLELKGF
                     DYETSEQVCTELLSDYGNVADHLNNRINEFTVNLNEANVNGIERIGEVNIYQSDPIVR
                     RASSLQLTRDAHYPRAIISPSLMEKLGLRVGDKITIKQGVGESQLEVEQSESIPDNCI
                     RIASAFNQTATLGGMFGEVSVKRM"
     gene            1972..3072
                     /gene="nuoH"
                     /locus_tag="PK522_03675"
     CDS             1972..3072
                     /gene="nuoH"
                     /locus_tag="PK522_03675"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNC40863.1"
                     /translation="MEYIQQWFEQLFGATLGSTLFLFAENLVYIVAIVLPLLLGVAYL
                     TFAERKIIAYMQIRLGPNRVTFFGISWLRGWGQPIADAVKAIMKEIIVPTGANKFLFL
                     LAPVITIAPALAAWAVVPFSPELVLADINAGLLYILAMTSLGVYGVIIAGWASNSKYA
                     FLGSMRSAAQVVSYELAMGFALVCVLMMSSSLNLGDIVIGQQGDSFLNWYLIPLFPMF
                     LVYFISGVAETNRAPFDVAEGESEIVAGFHVDYSGMAFTVFFLAEYANMILVATLTSI
                     MFLGGWLPPFNFAPFTYIPGMVWLLLKIAFILFFFLWFRATFPRYRYDQIMRLGWKIF
                     IPITLVWIVLIGMVMQLPVSLRDTFPLNMWFS"
     gene            3085..3573
                     /gene="nuoI"
                     /locus_tag="PK522_03680"
     CDS             3085..3573
                     /gene="nuoI"
                     /locus_tag="PK522_03680"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNC40864.1"
                     /translation="MERIKHFFKSFLLFELLKGMKVTGRYLFAPKITINYPEEKTPQS
                     PRFRGLHALRRYPNGEERCIACKLCEAVCPAMAITIESEQREDGTRRTTRYDIDMIKC
                     IFCGFCEEACPVDAIVETRVLEYHGEVRGDLTYTKEMLLAVGDQYEQQIAKDREVEAR
                     YR"
     gene            3584..4183
                     /locus_tag="PK522_03685"
     CDS             3584..4183
                     /locus_tag="PK522_03685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNC40865.1"
                     /translation="MSAQDTIFYFFSAVLILSALGVITFRNPVYAALLLVLAFFSAAG
                     IWLLLEAEFLAIALVLVYVGAVMVLFLFVVMMLDINLDKLREGFWKWFPFGALIAVVI
                     AVEIGLVMMGDQFKQMIPLQPKASDYSNTKELGRLIYTEYVYVFELAAVVLLVAMVAA
                     IALTLRHRTDKKSPNPSSQVKVKRNDRIRMVSMAPETKE"
     gene            4248..4553
                     /gene="nuoK"
                     /locus_tag="PK522_03690"
     CDS             4248..4553
                     /gene="nuoK"
                     /locus_tag="PK522_03690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNC40866.1"
                     /translation="MVSLSHYLILAAILFSIGVVGIFLNRKNVIIILMSIELMLLAVN
                     INFVAFSHFLQDTAGQIFVFFILTVAAAEAAIGLAILVVLFRNLRTINVDDLSELKG"
     gene            4598..6547
                     /gene="nuoL"
                     /locus_tag="PK522_03695"
     CDS             4598..6547
                     /gene="nuoL"
                     /locus_tag="PK522_03695"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNC40867.1"
                     /translation="MKELYLLVPLAPLLGAIIAGLFGRFIGTTWSHRVTILLVFVSLI
                     TSLIIFQDVIQAGNIFNGTIYTWLVLGETEFEVGFLIDPLSVTMMVIVNLVSLMVHVY
                     TIGYMQGDPGYQRFFSYISLFTFSMLMLVMSNNFLQLFFGWEAVGLVSYLLIGFWYTR
                     PTAIYANLKAFLVNRVGDFGFLLGIGLVLAYFGTLDYEIVFAHAEYMAKHSIEIIPGQ
                     SWMLLTVICLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVAR
                     MSPLFELSETALSTVIVIGGITTLFMALVAVVQNDIKRVVAFSTLSQLGYMTVALGAS
                     AYSIAIFHLMTHAFFKAVLFLGAGSVIIAMHHEQDMRRMGGLKKYMPITYWTMFIAAL
                     ASAGVPGFSGFFSKDAIIEAARFTEIPGGGFAYFCVLTTVFVTALYTFRLMIMTFHGK
                     ERMDHHTREHLHESPWVVTVPLIVLAIPTVVSGWIIGSLVFGDYFGNSIYFLSEHETM
                     MKLEAEFSGTFGMMLHALGTAPFWLSVGGIFTAWYLYAYKTEIPSKIKSLSGPIYNLL
                     DRKYYIDELYSLIFATGTRSLGTVLWKIGDVKIIDGLIVNGSAKLVGWVASWVRFFQS
                     GYIYHYAFTMIVGIFILMSLWLYHS"
     gene            6643..8127
                     /locus_tag="PK522_03700"
     CDS             6643..8127
                     /locus_tag="PK522_03700"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNC40868.1"
                     /translation="MLFGFPILSVVIWLPIIFGLFVLIIGNDHRVVLVRWISLLGSTL
                     GFIVALPLYTGFDSTLSSMQFVEQHIWFERFNVYYHLGIDGISMPLILLNCLITPLVV
                     IAGWEVIRDRTSQYFGAFLIMSGIVNGVFASLDAVLFYTFWEASLIPMFIIIGIWGGP
                     NRVYAAIKFFLYTLLGSLLMLVALLYLYHESEGSFSILSYHQLPLAMTPQILIFLAFL
                     LAFAVKVPMWPVHTWLPDAHVEAPTGGSVVLAAILLKLGGYGFLRFSLPIAPDASHYL
                     AEWMIILSLIAVVYIGLVALMQQDMKKLIAYSSVSHMGFVTLGFFLFNGYGIEGAMVQ
                     MISHGFISGAMFLCVGVLYDRLHSRQIADYGGVVNRMPIFAAFFMLFAMANAGLPGTS
                     GFVGEFMVIMGSVKVNFWYAFFAAMTLIFGAAYTLWMYKRVIFGEVANKNVEQLQDIS
                     LREGFLLTLLAIAVLWFGLYPFPLTEVMHATVDNLLEHLMNSKL"
     gene            8179..9624
                     /gene="nuoN"
                     /locus_tag="PK522_03705"
     CDS             8179..9624
                     /gene="nuoN"
                     /locus_tag="PK522_03705"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HNC40869.1"
                     /translation="MAFLSPDFSPAYPEIFLLIMICIVMLVDLFLDNDQRYFTYILSL
                     LSLAGCAVITFATLSSEVQYTFSGMFVDDLMSDSLKLMVYLTVAVVLIYSRNYIRVRG
                     MFKGEFFNLSLFATLGMMVMISASHFMVFYLGLELLSLSLYAMVALQRDSAVATEAAM
                     KFFVLGALASGFLLYGMSMIYGATGELHTSKIAEAIQTKTIDHQVLIIGLVFIVAGLA
                     FKLSAAPFHMWAPDVYQGSPTAVTLFLGSAPKLAAFGFTIRLLVDGLGALAADWQGML
                     IILAVASMAIGNIVAIMQTNIKRMLAYSTISHMGFVLLGFIAVDQNGYSSAMFYVIAY
                     VLMTLGAFGIIMILSREGFEAENLSDYKGLNQRNPWLALIMMFLMLSMAGIPPMIGFY
                     AKLSVLQAVVNAGFVWLAITAVVLSLIGAFYYLRIIKLMYFEAPEHDTPVLPSFDAKI
                     LISVNGLAVLIAGIFPQALMGLSLQAIQTSM"
     gene            complement(9638..10756)
                     /locus_tag="PK522_03710"
     CDS             complement(9638..10756)
                     /locus_tag="PK522_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNC40870.1"
                     /translation="MMHKIFFIILPLLISSFTYAETSNSQQSTNLPLEKIKLPPGFSI
                     QLWATIPDAKSLAIGRQGTVFVSSKTTGSIYAISQPDGKEKQVKMIANGLKSPSGITY
                     YEGALYVTALNRVFRFDEIENNLDQINEPVIIYDNLPKESFHSTRYMAVGHDNLLYIS
                     VGAPCDACEADPMRYALIARLNPNGSNFEVYAQGIRNSLGFDWHPKTHELWFSDIGRD
                     WMGEDLPPDELNYAATQGLHFGFPNCHAGKILDPKYGAKRGCDKSQAPIAELEPHVSP
                     QGIKFYSGRMFPPEYHDQLIIAEHGSWNRRAPVGFRLQHFQLKNNQIVKKEIFAEGWF
                     NDQKVWGRPTDLLVMPDGALLVSDDLAGAIYRISYQDN"
     gene            10893..12818
                     /gene="htpG"
                     /locus_tag="PK522_03715"
     CDS             10893..12818
                     /gene="htpG"
                     /locus_tag="PK522_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNC40871.1"
                     /translation="MQASTQQKERMNFQAEAKQLLKLMIHSLYSNKEIFLRELISNAS
                     DAVDKLRFEGLTDSALYESDSDFKIRVGYDKDARTVTISDNGIGMSRQEVIDHIGTIA
                     KSGTREFFNSLTGDQAKDAHLIGQFGVGFYSAFIVADKVTLITRRAGLTPEHGVRWES
                     QGEGDYELETVEKRDRGTEITLHLREGEDDFLSGFQLKSIIRRYSDHITLPVMMKKEE
                     WVQESNEYKITDEDETINQASALWARSKNEITQEQYEAFYKHVAHDYEPPLAYVHAKV
                     EGKQEYTQLLYIPSRAPFDLYDREHRHGIKLYVKRVFIMDDAEKLLPNYLRFVRGIID
                     SSDLPLNVSREILQESKDIDNIRAGSVKKILSLIEDFAGSSKEEDQAKFRTFWKEFGQ
                     VLKEGVGEDYANRERIAKLIRFVTTSSDSDEQTVSLDDYIARMKEGQEKIYFVTADNL
                     KAARSSPHLEIFRKKGIEVLLLSDRIDEWLVSNLTEYAGKALQSVAKGSLDLGKLEDE
                     VEKQEQEKEAEAYKDLISRIKEVLGERIKDVRITYRLTESPACLVADMYDMSGNLERL
                     LKSAGQKVPITKPILEINPHHPMVQRLKYEEEKFSDWSNILFDQALLAEGGQLEDPAA
                     FVKRLNDLLLQTILIAK"
     gene            12902..13378
                     /locus_tag="PK522_03720"
     CDS             12902..13378
                     /locus_tag="PK522_03720"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNC40872.1"
                     /translation="MEDDKAYQAKLLTPFAVLGIRTEEDWLTGIDYLPRDIPEKIPQN
                     VLAKEVCLQLRAYLKDPQFTFDLLLHISGTHHQQRVWSSILEIPPGKTRSYAEIAKQI
                     HSAPRAVGQACGANRLPIVIPCHRVIAKNGGLGGFMHASDGMPLDIKRWLLRHESL"
     gene            13375..14307
                     /gene="xerD"
                     /locus_tag="PK522_03725"
     CDS             13375..14307
                     /gene="xerD"
                     /locus_tag="PK522_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNC40873.1"
                     /translation="MMLCDSDVKLIDEFIDSLWLEDGLSRNTLSSYRSDLKQLSLWKL
                     NNKSKQLSIAELDHSDLLAFLADRVAILIKASSANRELSTIKRFYQFLLRQRKIKIDP
                     SLNIDRPKLPRLLPVSLSETEVEILLSAPDILKSQGIRDRAMLELLYATGLRVSELVT
                     LQLLQVRVDMGVVKVLGKGNKERLIPFGEEALYWVNRYITEVRNSLLAGKNKITDKVF
                     ITNRGEAMTRQAFWYLIKRYAQHTGITKSLSPHQLRHAFATHLLNHGADLRVVQLLLG
                     HADISTTQIYTHIARERLKQLHARHHPRGSIFQQ"
     gene            14685..16049
                     /locus_tag="PK522_03730"
     CDS             14685..16049
                     /locus_tag="PK522_03730"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNC40874.1"
                     /translation="MGLKIAFSDKPFRKLWLIFAIISNLLLLGYFKYADFFISTVNYI
                     SSANVQTLSIILPIGISFYTFTQIAFLVDTYEGKVKEYRFIHYLLFVTYFPHLIAGPV
                     LHHKEMMPQFADSRIYRFSQSDFAVGITIFCIGLAKKVLIADNLAPYANNLFNEANSS
                     PSLLIAWGGVLAYTFQLYFDFSAYSDMAIGLSRLFGVNLPLNFNSPYKSRNISEFWRR
                     WHMTLSRFLRDYLYIPLGGNRVGLFKQYRNLMTVMLLGGLWHGAGWSFVIWGGLHGAY
                     LIIHHGWVKLSQKIQFPVTTMLWQIAATGLTFMSVCIAWVFFRSAELEDALEIIYGMF
                     GVFGIELPDAIGSRLIILAPFIQELGITFYLGGGKIFIETWSWIALAALIAFFFPNTQ
                     EIMQNFKPALDFQNSKIKSPTRNLLGYLVWQPSRWWSVWIGVITVISLLSLNRPNDFL
                     YFQF"
     gene            16058..17314
                     /locus_tag="PK522_03735"
     CDS             16058..17314
                     /locus_tag="PK522_03735"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNC40875.1"
                     /translation="MEELQNSQRYLCWLLVITIAGSLAVVIFVLIVDPYRQYGIVQYR
                     GFNIIKPTLERYQNEIKLSQVKRLKPDVLILGNSRAEIGFDPDAPVFNQKGLSAYNLS
                     IPGIGIETVYQQLLYLTKNGINPGLIFLGLDFLDFIDLPSQQEVNASIDPSQYLIDQQ
                     RKIESWFWRFDSMFSLASVKDAFRTLAIQNNEEAAIVTFHGFNPLNEYKPIVRAEGYY
                     KLFQQRAQENVRTYLKKAQGSVSPMSLNYLQAIFNLVEENNSKIILVIYPYHAQILAL
                     FEVTNLWPLFNQWKSLVVNELIDMNQYSAQIELYDYSGYSLYHCERIPPIGDLLSTTQ
                     WYWEAGHFKKELGDIILEEILRSNETILSKVMSMNHSQTSFGIRLLDQNSFLLNQNRI
                     IQQRLMCESNYPELFTDAAILARAAQ"
     gene            complement(17409..18107)
                     /locus_tag="PK522_03740"
     CDS             complement(17409..18107)
                     /locus_tag="PK522_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC40876.1"
                     /translation="MATTLITGSNRGIGFEFARQYAQSGWNVIACCRQPENASNLRQL
                     ANSFPDLVTIYPLDVADHNQITNLSNNLRNTSIDLLINNAGIYPPSDDGGFGKIDYEA
                     WHKAFQINTMAPLRMAEAFLAQISNSQLKIIAIITSKMGSIDDNKRGGSYIYRSSKAA
                     VNMVGKSLSVDLKLQGITTVILHPGWVRTDMGGPDALISPEQSVLGMQRILSNLKLSD
                     SGKFFAYDGKIIPW"
BASE COUNT         5209 a         3060 c         3858 g         6080 t
ORIGIN      
        1 attgcacacg gtgtgttcga tttgggcaag aaattgctgg aattatggaa cttggcatga
       61 gtggccgtgg tgagcattct gaaattctat cttttgttgg gaaaacagtt gattccgagc
      121 tgtcagggaa tgtgattgat ctttgtcctg ttggagcatt agtcagtaag ccatttcgat
      181 attcagctcg aacatgggaa ttatctcgca gaaaatcgat cagccctcat tgtgggttag
      241 gtagcaattt gattgttcaa gttaaacaaa atcgtgtcat gcggattttg cctcgagaaa
      301 atgaggagat taatgaatgt tggttgtccg ataaagatcg attttcatac gaaggtctga
      361 attctgaaga tcgcttatct aagccgatga tcaaaagaaa taatcaatgg ataacttgtg
      421 attggcaaga agcgctgtta tttgtggttc agaatctgaa agcttgtgtt agtcaatatg
      481 gtggaagagc gattggcgca ctgggttccc ctcatagcac gcttgaagaa ctttatctgt
      541 tgcaaaaatt agtaaggggg ttgggaagta acaatatcga tcatcgcttg agacaatctg
      601 attttcgtgc tgataactac cagcaaggga tcccatggtt aggtatgcct attgctgaag
      661 tttcaaattt aaaatcgata ttaattattg gtagtaattt gcggaaagat catccacttt
      721 tagcattgag ggtgcgtcaa gcagttaaaa gtggagcaca gttaagtata gttaattcta
      781 ctgatgatga tttgctgacg aaagtagctc aacgtgcaat cgttgctcct tctgaattgg
      841 taagaacatt agcacaaatt cttaaagcga taattgagta tcgatatgtt gagattgcag
      901 ataagattaa agaaacgctt acaaaagtaa acgttacgga tgtaacaaaa gcgattgcaa
      961 ataatttagt atcgaatcaa tctgctggaa tttttcttgg gaatttatct cagcatcatc
     1021 ctaattacag tgagattttt ttactcgcac aagaaatagc caaagttact agcgccaaat
     1081 tgggtgtttt tggggaagca gccaatagcg taggcgcata cgtggcaggt gcgatcccta
     1141 ataggaaatt gggtaatttg acaacaaatg gagaagaggt tggattaaat gcaatccaaa
     1201 tgctgaatgc tgaagcagtc agtgcatgta aagcctatat tctagtgaat ttagaaccag
     1261 aatatgatac ttacgatcct caatcagcgc ttgaacgaat taagggtgca gaatttgtag
     1321 tatcgctgag tagttataaa ggtaacatca ccgattatgc tgacgttatt ttgcctattg
     1381 caccatttac agaaacatca ggaacattta ttaatacaga agggcgtgtt caaagcttta
     1441 acggggtagt ttctccctta gcagaagcga gacccgcttg gaaagttttg cgagtattag
     1501 caaatctgct ggaattgaaa gggtttgatt atgaaacatc tgagcaagtt tgtaccgaac
     1561 ttttgtctga ttacggtaat gtagctgatc atttaaataa taggatcaat gagtttacag
     1621 ttaatttaaa tgaggccaat gtgaatggta tcgaacggat tggagaggta aatatctatc
     1681 agtctgatcc gattgtacgc cgtgcctcgt ctttacaatt aacgcgtgat gctcattatc
     1741 ctcgagcaat catatcgcct tccttgatgg agaaattagg actgagagtc ggtgataaaa
     1801 taacaataaa gcaaggagtt ggtgaaagtc agctagaggt tgagcagagt gaatcgattc
     1861 ctgataactg cattcgtatt gcaagtgctt ttaatcagac tgcgacatta ggtggtatgt
     1921 ttggagaagt atcggtcaaa cggatgtaat tgagcaaatt aaggtctgtt aatggaatat
     1981 atacaacaat ggtttgagca actgtttggt gccactttgg gatcaacctt gtttttgttc
     2041 gctgagaatc tagtctatat tgttgcaatt gttttgcctt tacttttagg cgttgcttac
     2101 ttaacgtttg cagaaagaaa aatcattgct tatatgcaaa ttaggttggg gcctaatcga
     2161 gtcactttct tcgggatttc ttggttaaga ggatggggac agccaatcgc tgatgcagta
     2221 aaagcaatta tgaaggaaat tattgttcca actggagcca ataaattctt atttcttctt
     2281 gcaccagtaa ttacgattgc gcccgcgctt gctgcatggg ctgttgttcc tttctcccca
     2341 gagttagttt tagccgatat caacgcgggt ttactttata ttcttgctat gacgtcttta
     2401 ggggtgtatg gcgtcatcat tgccgggtgg gcttctaatt caaagtatgc tttcctggga
     2461 tcgatgcgct cagcagccca ggtagtttca tatgagctcg caatggggtt tgcgctggtt
     2521 tgcgtgctca tgatgtctag tagcttaaat cttggtgata ttgtgatagg gcagcagggg
     2581 gatagttttc tgaattggta tttaatacca ttatttccga tgttcttggt ttactttatt
     2641 tctggtgttg ctgaaacgaa tcgtgctcca tttgatgtag cagaaggtga atctgaaatc
     2701 gtagctggat ttcatgtaga ttattcaggt atggccttta ctgtcttttt tctagctgaa
     2761 tacgcaaaca tgatattagt cgcaacattg acaagcatca tgttcttggg cgggtggtta
     2821 ccaccattta attttgcacc attcacttat ataccaggaa tggtttggtt attactgaag
     2881 attgccttta tattgttttt ctttttatgg tttcgcgcaa cattccctcg ttatcgatat
     2941 gaccagatta tgaggctagg ttggaaaatt tttatcccaa ttaccttggt ttggatagtt
     3001 ttgattggaa tggttatgca gttaccggtt tcactacgtg atactttccc tcttaatatg
     3061 tggtttagct gaggtattaa ctatatggaa cgaatcaagc acttttttaa atcatttctt
     3121 ttatttgagt tattaaaagg aatgaaagtc actgggcgat atttatttgc acctaaaatt
     3181 actattaatt accctgaaga aaagacgcca cagtctcccc gtttcagggg attacatgca
     3241 ctgcgtcgtt accctaatgg tgaggaacgt tgcatagcat gtaaactatg cgaagctgtt
     3301 tgccctgcga tggcaattac cattgaatcc gaacagcgtg aggatggtac tcgtcgcacg
     3361 acacgttacg atattgatat gatcaaatgc atcttttgtg gattttgtga ggaagcttgt
     3421 cctgttgatg ccatcgttga aacccgtgtc cttgaatatc atggtgaagt tcgtggtgat
     3481 ctaacttata caaaagagat gctgctcgcg gttggagacc aatatgagca gcaaattgca
     3541 aaggatcgag aagtagaggc acgctaccga taatttttta tatatgagcg cacaagatac
     3601 aattttttat ttcttttcag ctgttctgat tttatcagca ctcggagtta ttacgttccg
     3661 aaatccagta tatgctgcat tactattggt attggcattt ttttctgctg ccggtatatg
     3721 gttactatta gaagctgagt ttctagccat tgcgttagtc ttagtttatg taggtgcagt
     3781 catggtttta ttcttgtttg tagtgatgat gctagatatc aatctagaca aattacgaga
     3841 agggttttgg aaatggtttc cttttggagc actaatcgca gtagttattg ctgttgagat
     3901 aggtttagta atgatggggg atcaatttaa gcagatgatt ccgcttcagc cgaaagcctc
     3961 ggattatagt aataccaagg aattaggtcg attgatttat actgaatatg tttatgtttt
     4021 tgaattggca gcagttgttt tacttgttgc aatggttgca gcaattgcat tgacattaag
     4081 gcaccgtacc gataaaaaat cacctaaccc atctagtcag gttaaagtaa aaaggaatga
     4141 tcgtattaga atggttagca tggcacccga aacaaaggaa tgagtgccat cataggttga
     4201 tataaaggat tttggcggta aattaataaa aattataggt gaaaattttg gtctctttat
     4261 ctcattacct catacttgct gcaattttat tttctattgg tgtcgttgga atttttctca
     4321 atcgaaaaaa tgtaattatc atcttgatgt ctatagagct aatgttactg gctgttaata
     4381 tcaactttgt tgctttttct cattttctac aagatacagc gggtcaaatt tttgtattct
     4441 ttattctgac agttgccgca gcagaagcag caattggact cgcaatttta gtagtgctgt
     4501 tccgaaatct tcgaacgatt aatgtagatg atttgagtga actcaaaggg tagatatttt
     4561 tattaaaaga ttactaaaaa tttaatttat tagctcaatg aaagaactgt atttgttagt
     4621 gccattggcg ccattactag gagccatcat agcggggtta tttggacgct ttattggaac
     4681 aacttggagt catcgtgtaa caattctgct ggtttttgta tcactcatta catcattaat
     4741 tattttccaa gatgttattc aggccggaaa tatttttaat ggaacaattt atacctggtt
     4801 agttttaggc gaaacagaat ttgaagttgg ttttttgatt gacccgcttt cagtaacgat
     4861 gatggtaatc gttaatttgg tttcgctgat ggtgcatgtt tacacaatcg gttatatgca
     4921 aggtgatcca ggttaccaac gtttctttag ttatatatca ctattcacct tctcaatgtt
     4981 gatgttagtg atgtccaata atttccttca attatttttt ggttgggaag cagtaggatt
     5041 agtatcgtat ttattgattg gattctggta taccagaccc acggcaatct acgcaaattt
     5101 aaaagcattt cttgtcaatc gcgtaggtga ttttggtttt cttttaggaa ttggtctagt
     5161 actcgcttat tttggtacat tagattatga gatcgttttt gcgcatgcag aatatatggc
     5221 gaagcactca atcgaaatta ttcctggaca gtcctggatg ttattgaccg taatttgctt
     5281 gttattattt gtaggtgcaa tggggaaatc tgcacagttt ccactacatg tatggctacc
     5341 tgattcaatg gagggtccta caccgatatc cgcattgata catgctgcaa caatggtgac
     5401 cgcaggcatt tttatggttg cgagaatgtc tccccttttc gaattatctg aaacggcttt
     5461 atcaacagtt atcgttattg gtggaatcac aacactattt atggcactag tcgcagtggt
     5521 tcagaatgat attaaacgag tggttgcatt ctcaacacta tcgcagcttg ggtatatgac
     5581 tgtcgcttta ggagcatcgg cctactcaat cgcaattttt catctcatga cgcatgcgtt
     5641 cttcaaagcg gtactatttc tcggagcagg atcggtgatc atagcgatgc accatgaaca
     5701 agatatgaga agaatgggtg gattaaaaaa atatatgccc atcacttact ggactatgtt
     5761 tatagctgca cttgcgagcg ccggtgttcc aggtttctca gggttctttt caaaggatgc
     5821 cattatcgaa gcagcaagat ttactgaaat cccaggaggg ggattcgctt atttttgtgt
     5881 ccttacaaca gtattcgtta ccgcgcttta taccttccga ttgatgatta tgaccttcca
     5941 tggtaaggaa cgcatggatc atcatacgcg cgaacatctt catgaatctc cctgggttgt
     6001 aactgttcca ttaattgtat tagcgatacc aacagttgtc tcaggttgga ttataggatc
     6061 tttagtgttt ggtgattact ttgggaattc gatttacttt ttatctgagc atgagacgat
     6121 gatgaaattg gaggcagaat tctctggtac ttttggaatg atgctacatg cacttggaac
     6181 tgctccattc tggctttcag ttggaggaat ttttacagct tggtatcttt atgcctataa
     6241 aacagagatt ccttctaaaa ttaaatccct atcaggccca atttataatt tacttgatcg
     6301 gaaatactat attgatgagc tttattcttt aatatttgcc actggaacgc gatcgctagg
     6361 aaccgtgttg tggaaaatag gagacgtgaa aattattgat ggtctcattg taaatggctc
     6421 agccaaattg gttggatggg tcgcttcgtg ggttcgcttc tttcaatcag gttatattta
     6481 tcattacgca tttacaatga tagtagggat ttttattttg atgagtttat ggttgtatca
     6541 ttcataatgg tgatttcagc tatctatctt caattttatc tatcattagt tgcatcgaag
     6601 agaaaaaata ataaactcta attttaatca agtggatttg tcatgctgtt tggatttcca
     6661 attctaagtg tagtcatctg gttacctatc atctttggtc tctttgtatt aatcatcgga
     6721 aatgaccata gagtggtatt agtacgttgg atttctcttt taggttcaac tttaggtttc
     6781 attgttgcac tgcctttata tacgggattt gattccactt taagtagcat gcagtttgtg
     6841 gagcaacata tttggtttga acgttttaat gtttattatc atcttggcat tgatggaatt
     6901 tccatgccat tgatactact caactgctta attacacctt tagttgtaat tgcaggttgg
     6961 gaagtaatac gcgatcgcac ctcacaatat ttcggtgcat tcttgattat gtcagggatc
     7021 gttaatgggg tgtttgcgtc attggatgcg gtacttttct atactttctg ggaagcatcc
     7081 ttgattccaa tgtttattat tattgggatt tggggtggtc ccaaccgagt atatgctgca
     7141 attaagtttt ttctgtatac actcttaggt tcgttgttga tgctggttgc cttactctat
     7201 ttgtatcatg aatcggaagg cagcttctca atattaagtt atcaccagct tcctctggcg
     7261 atgacaccgc aaattcttat attcctagca ttcttgttag cgtttgctgt aaaggtaccc
     7321 atgtggccag tgcatacatg gttaccggat gcacacgtag aagcgcctac aggtggttct
     7381 gttgtgcttg ctgcaatttt attgaaatta ggtggttatg gatttttaag attctcatta
     7441 ccgatcgcac ctgatgcaag ccactatttg gctgagtgga tgattatttt gtcgctgatc
     7501 gcagtagttt atattggtct tgttgcatta atgcagcaag acatgaaaaa attgattgcc
     7561 tattcatctg tctcccatat gggttttgta actttgggtt tctttttatt caatggctat
     7621 ggaatagaag gtgcaatggt gcaaatgatt tctcatggtt ttatttcggg tgccatgttt
     7681 ttatgcgttg gtgttttgta tgacagacta cattcacggc aaatagctga ttatggtggt
     7741 gttgttaatc ggatgccgat ttttgctgca ttttttatgt tgtttgcaat ggctaatgct
     7801 ggactgcctg ggactagtgg tttcgtggga gaattcatgg taattatggg ttctgtgaaa
     7861 gttaattttt ggtacgcatt ttttgctgcg atgacactca tttttggcgc agcttataca
     7921 ttgtggatgt ataaaagggt aatttttggg gaagtagcca ataaaaatgt agaacagctt
     7981 caggatattt cattacgcga gggttttctg ctaaccctac tggccatcgc agtgctatgg
     8041 tttggactgt atccatttcc actcacagaa gtgatgcacg caacagttga taatttactg
     8101 gaacacttaa tgaatagtaa gttgtagcct tgcgaattaa taaaataata caagaattta
     8161 gatcaaaaag gataatagat ggcgtttttg tcacctgatt tttcaccagc atacccagaa
     8221 atctttctac tcataatgat ttgtatcgtt atgctagtgg atcttttctt ggataatgat
     8281 caaaggtatt ttacttatat cctctcgctg cttagtttgg caggttgtgc agtgattacc
     8341 ttcgcgactt tatcaagtga agtccagtac actttctcag ggatgtttgt cgatgatcta
     8401 atgtcagata gtctgaaact catggtatat ctgaccgtag ctgtggttct gatttattct
     8461 cgtaactata ttcgtgtaag aggtatgttc aaaggggagt tttttaacct ctctttattc
     8521 gctaccttag gaatgatggt tatgatttct gcaagtcact ttatggtttt ttatctaggc
     8581 cttgagttgt tatcgctttc tttatatgcg atggttgctc tccagcggga ttctgcagta
     8641 gcaacagaag ctgccatgaa gttttttgtt ttaggtgcgt tggcgtctgg ttttctgttg
     8701 tatggaatgt caatgatata cggtgcaacc ggtgaattac atacgagcaa aattgctgaa
     8761 gcaatccaaa caaaaacgat tgatcatcaa gtattgataa tcgggttggt ttttattgta
     8821 gcgggtctgg cttttaaatt gagtgctgca ccatttcata tgtgggcacc tgatgtttat
     8881 caagggtctc ctactgcagt aacactattt ctgggttctg ctccaaaatt agctgcattt
     8941 gggtttacta ttcgattatt ggttgatggt cttggtgctt tagcagccga ttggcaaggc
     9001 atgctaatta tattagcggt tgcttcgatg gcaattggta atatcgttgc gattatgcaa
     9061 acgaatatta agcgcatgct tgcttattct acgatttcgc atatgggatt tgttttactc
     9121 ggttttatcg cagtagacca gaatggctat agctcggcta tgttctacgt tatcgcttat
     9181 gtactcatga cgttgggtgc atttggaata atcatgatct tatcccggga aggttttgag
     9241 gcagagaatc ttagtgatta taaaggtctt aatcagagaa acccgtggtt ggcattgatc
     9301 atgatgtttc taatgttatc gatggcaggt attcctccta tgattggttt ctacgcaaaa
     9361 ctatccgtgc tgcaggcggt tgtaaatgct ggtttcgtat ggctagccat aacagcagtt
     9421 gtgttatcgc tgatcggtgc gttctattac ttgcgtatta ttaagcttat gtattttgaa
     9481 gcacctgaac acgacacacc tgtattacct tcttttgatg caaaaatact gatcagtgta
     9541 aatgggttgg cagttttaat tgccggtatt tttcctcaag cacttatggg tcttagcctt
     9601 caagccattc aaacctcgat gtagcatctc atcttaatca gttatcctga taactgattc
     9661 gataaatagc gcctgctaga tcgtcagaga ctaatagcgc gccatctggc attactagta
     9721 aatcagttgg tcttccccaa accttttggt cgttaaacca accttctgca aatatttcct
     9781 ttttaacgat ctgattattt tttaattgaa agtgctgtaa acgaaatcca acaggagctc
     9841 tacgattcca tgatccgtgc tctgcaatga tgagttgatc gtggtattca ggaggaaaca
     9901 tacgcccaga ataaaattta atgccctgag ggcttacatg cggttctagt tcagcaatgg
     9961 gtgcttgaga cttatcacaa ccccgctttg caccatattt aggatcaaga attttgccag
    10021 catgacaatt aggaaaacca aaatgcaaac cctgagttgc agcatagttg agctcatcgg
    10081 gtggaagatc ttctcccatc caatctcttc caatatcaga aaaccatagt tcatgggttt
    10141 tgggatgcca gtcaaagcct agcgaattgc ggataccttg tgcatagact tcaaaattac
    10201 tcccattagg attgagacga gcaatcagcg cgtagcgcat tggatctgct tcacaggcat
    10261 cacaaggagc cccaactgaa atatataata agttatcatg accaacagcc atgtaccttg
    10321 tgctatgaaa ggattcctta ggtagattgt cataaataat tactggttca ttaatttgat
    10381 caaggttatt ttcgatttca tcaaatcgaa aaactcgatt gagagcagtt acatagagcg
    10441 caccttcata ataggttatt ccgctgggag attttagacc atttgctatc attttgacct
    10501 gtttttcttt accatcaggt tgtgagatag cataaatact tcctgtagtt ttagagctaa
    10561 caaatactgt gccttgtcta ccaatcgcta atgatttagc gtctggtatc gttgcccata
    10621 gttggattga gaagccgggc ggtaatttta ttttttctaa aggtagattt gttgattgtt
    10681 gcgagtttga agtttcagca taggtaaagc tgctaataag caggggtaat ataataaaaa
    10741 aaattttatg catcatggaa tattatttag tttaggcaaa attaactaag aaaaagaata
    10801 ataaaatact agtttcttga tgttaccgat ttagcacttg aattttgata ttttaatcat
    10861 acaatcttac cgcgttttta aggagaaaat acatgcaagc atcaacacaa cagaaagaaa
    10921 gaatgaattt tcaggctgaa gccaagcaat tacttaaatt aatgattcat tctttatata
    10981 gcaacaaaga aattttctta agagaattga tttcaaatgc atctgatgca gtagataaac
    11041 tacgctttga agggctaact gattcggcct tatatgaatc ggactccgat tttaaaattc
    11101 gagtgggcta cgataaagat gctagaactg tgactattag tgataacggg attggcatgt
    11161 cgcggcagga agttattgat cacattggca caattgcaaa atcaggaacg agagagtttt
    11221 ttaattcttt aacaggcgat caagccaaag atgcgcactt aattggtcaa tttggagttg
    11281 gcttttattc tgcttttatt gtggctgata aagtaacctt aattacgaga agagcaggac
    11341 taactcctga acatggtgtt cgttgggaat ctcaaggaga aggagattat gagttagaaa
    11401 cagttgagaa gagggatcgg ggtacagaaa ttactttaca tttaagagaa ggtgaagatg
    11461 atttcttaag tggttttcag ctgaaatcaa ttattcgacg ttattcagac catatcactt
    11521 tacctgtgat gatgaagaaa gaagaatggg tccaagaaag taatgagtat aaaattaccg
    11581 atgaagatga aactattaat caagcgagtg cactgtgggc tcgatcgaaa aatgaaatta
    11641 ctcaagaaca atacgaagcg ttttataagc atgtagcaca tgattatgag ccaccgcttg
    11701 cctatgtaca tgccaaagta gaaggaaaac aagaatatac acagttatta tatattcctt
    11761 cgcgcgcacc ctttgattta tatgatcgcg aacatcgtca tgggattaaa ttatatgtga
    11821 agcgcgtatt tatcatggat gatgcagaaa aattacttcc taattatctt cgattcgtcc
    11881 gtggaatcat tgattctagc gatttacctt tgaatgtttc tcgagaaatt ctgcaagaat
    11941 caaaagatat cgataacatt cgagctgggt cagtcaaaaa aatacttagc ttaatcgaag
    12001 attttgctgg gagcagtaaa gaggaagatc aagccaaatt taggacattc tggaaagaat
    12061 ttggtcaagt tttaaaagaa ggagtaggtg aggattacgc aaaccgggag cgtattgcta
    12121 agttaattcg atttgtaacg acgagtagcg atagtgatga acaaaccgtt tcattggatg
    12181 attacattgc tcgcatgaaa gaaggtcaag aaaaaatata ttttgtaaca gcagataatt
    12241 taaaggctgc gagaagcagt cctcatcttg aaattttccg taagaaagga atagaggttt
    12301 tattgttatc tgatcggatc gatgaatggt tagtctcaaa tttaactgag tatgctggta
    12361 aagctttaca gtctgtggcg aaaggaagcc ttgatctcgg taagcttgaa gatgaagtcg
    12421 agaaacaaga gcaggaaaaa gaagcggaag cctataaaga tttaatttca agaataaaag
    12481 aagtattggg agagaggatt aaagacgtac gtatcactta tcgtttgaca gaatcgccgg
    12541 cttgcttggt agcagatatg tatgatatga gtggtaatct cgaaagatta ctcaaatctg
    12601 caggtcagaa agttcccatt accaaaccaa ttttagaaat taacccgcat catccaatgg
    12661 ttcagcgctt gaaatatgag gaagaaaaat tctcggattg gagcaatatt ctgtttgatc
    12721 aggcattact tgcagaagga ggacaattag aggatccggc tgcttttgta aaacgcttaa
    12781 acgatttact tttgcaaact attttgattg caaagtaaat gagagtaacg cttcataatc
    12841 agtaaattag tttgcttata gcgaggaagt agcattggga agtgataagt caagtgtgaa
    12901 aattgaagat gacaaagctt atcaagctaa attacttaca ccttttgcag tgctaggcat
    12961 aagaacagaa gaggattggt taacggggat tgattatcta ccgagagata tacccgaaaa
    13021 aataccacag aacgtattgg ctaaagaggt atgcctacaa ttgagggcgt acctcaagga
    13081 ccctcaattt acgtttgatt tattattaca tattagtggg actcaccatc aacaaagagt
    13141 ctggtctagt attctcgaga ttccacccgg aaaaacgaga agctacgcag aaattgcaaa
    13201 acaaatccac tcggcaccac gtgccgttgg tcaagcatgt ggtgccaacc gtttgcctat
    13261 tgtgattccc tgtcatcgag tcattgcgaa gaatggtgga ttaggtggct ttatgcatgc
    13321 cagtgatggt atgccgctcg atattaaacg ctggctgctc aggcatgaaa gcttatgatg
    13381 ctatgtgata gtgatgttaa gctcattgat gaattcattg attcattatg gttagaagat
    13441 ggattatcga ggaatacact ctcaagttac cgcagtgatc taaaacaact ttcgttatgg
    13501 aaacttaaca ataagagcaa acagctatcg atagctgaac ttgaccactc agatttactt
    13561 gcttttctcg ctgatcgagt cgcaatttta ataaaagcaa gttctgcaaa tcgagaatta
    13621 tcgacgataa aacggtttta tcagttttta ttacgtcagc gaaaaatcaa gatcgatcct
    13681 agtttaaata ttgatcgacc aaaactaccg cgattgcttc cagtaagttt atctgaaact
    13741 gaagtagaaa tacttttgag tgcacctgat attttaaaat cacaaggaat tcgcgatcga
    13801 gctatgctag agctgctcta tgccacgggt ttacgtgttt cagagttggt gacacttcaa
    13861 ttactgcagg ttagagttga catgggcgtt gtgaaagtac taggtaaggg taataaagaa
    13921 cgattgatac cgttcggtga agaagcactt tattgggtta atcgctatat aaccgaggtt
    13981 cgtaacagct tattggcagg aaagaataag ataaccgata aagtttttat taccaatcga
    14041 ggcgaggcga tgactcgcca agcattttgg tatttgatta aacgctacgc tcagcataca
    14101 ggtattacta aatcactttc tccacatcag ttacggcatg cttttgcaac ccatctcctg
    14161 aatcacggtg ctgatttaag agttgttcaa ctgctacttg ggcatgctga tatttctaca
    14221 actcaaatct atacccatat tgctcgtgag cgtctgaaac aattacatgc gcgccaccat
    14281 ccaagaggtt ctatttttca acagtaaatt gattttataa agcgccaatt ttgttcattt
    14341 tttaataatt gaaatattgc tagaagcagt atgatggtta ttaattcttt ctaaattagc
    14401 ttggaatagg ataggttatc tttaatagaa cagttgattg gagaaaaatg aagaatggaa
    14461 tgatgtttgt ggaaaaataa ggccaattat tcttaatgct atttaattct tttactttta
    14521 tattttttta tttgcccatt accgttgtgg gtttcttttg gctgagccgc tatagttata
    14581 ctttatcagc tgggtggtta gcatctgctt caatagtttt ttatgggtat tgggactatc
    14641 actacatacc acttcttcta agttcaatct gctttaattt ttggatgggg ctcaagattg
    14701 cattctcaga taaaccattc cggaagcttt ggcttatttt tgcgattatc tcaaatctgt
    14761 tattactcgg gtattttaag tacgcagatt tttttatttc tacagttaat tacattagta
    14821 gcgcaaatgt tcaaactttg agcattatct tgcctattgg catttctttt tatacattta
    14881 ctcagattgc attcctcgtg gatacatatg agggaaaagt taaagaatat cgcttcattc
    14941 attatttact atttgttact tattttcctc atcttattgc tggaccagta ttgcatcaca
    15001 aagaaatgat gcctcaattt gctgattcga gaatatatcg tttttctcaa tctgattttg
    15061 cagtaggaat aacaatattt tgtattggtt tggcaaagaa agttttgatt gcagataacc
    15121 ttgctcccta cgcaaataat ttatttaatg aagcgaactc ttccccatca ttactaatag
    15181 catggggagg tgtattagct tatacgttcc aactctattt tgatttctca gcttattcag
    15241 atatggcaat tggtttatcg cgactatttg gagtgaatct tccactaaat tttaattcac
    15301 cctataaatc aagaaatatt agcgaatttt ggcgaaggtg gcacatgact ctgtcacgct
    15361 ttcttagaga ttatctatat attccacttg gtggtaacag ggtagggtta ttcaagcagt
    15421 atcgaaattt aatgactgtt atgttattag gtggtttatg gcatggagct gggtggagtt
    15481 ttgttatatg gggtggactt catggagctt atctaattat tcaccatgga tgggtcaagc
    15541 tttcacaaaa aatacaattt ccagtaacaa caatgttatg gcagatcgct gctactggtc
    15601 taacatttat gtcagtttgc attgcctggg ttttttttcg ttcagcagag ctggaagatg
    15661 cattagaaat tatttatggg atgtttggag tttttggtat agaactacct gatgcaattg
    15721 gtagtcgact tataatattg gccccattca ttcaagaatt aggcataaca ttttatctag
    15781 gaggggggaa aatttttatt gagacttggt cttggattgc tttagctgca cttattgctt
    15841 tctttttccc taacacacaa gaaattatgc aaaattttaa acctgctcta gattttcaaa
    15901 actcgaaaat taaaagtcca actcgaaatt tgctaggtta tttagtttgg caaccatcac
    15961 gctggtggtc agtatggatc ggggtaatta ccgtgataag cctattgtca ttaaatcgtc
    16021 cgaatgattt tttgtatttt cagttttaat tcagagtatg gaagaattac agaattcgca
    16081 gcgatatctt tgctggctat tagtgattac gattgctggt agcctagcgg tagtaatttt
    16141 tgtacttatc gttgatcctt atcgccagta tggcattgtt caatatcgtg gatttaatat
    16201 tattaaaccc acattagagc gctatcagaa cgagattaaa ttatcccaag taaagcgact
    16261 gaagcctgat gtacttattt taggtaattc tcgcgctgag atcggatttg atcctgatgc
    16321 accagtgttt aatcaaaaag ggctatcagc ctataattta agtataccgg ggataggaat
    16381 tgaaactgta tatcaacagc ttctttattt gacaaaaaat ggtattaatc cggggttgat
    16441 ttttcttggc cttgattttt tagattttat tgatttaccg tctcagcagg aagtcaatgc
    16501 atcaattgat ccttctcagt atttaatcga tcaacaaaga aaaattgaaa gttggttctg
    16561 gcgttttgat agcatgtttt ctctagcttc cgttaaggat gcctttcgta cacttgctat
    16621 tcaaaacaat gaagaagctg caatcgttac ttttcatggg tttaatccac ttaatgaata
    16681 taaaccaatt gtacgagctg aagggtatta caaattattt caacaaagag cacaagaaaa
    16741 tgttagaact tatcttaaaa aagcgcaagg ctcagtgtcg ccaatgagtt tgaattactt
    16801 acaggctata tttaatctag tagaggagaa taatagcaaa attattcttg tgatttaccc
    16861 ctatcatgcc cagatactag cactttttga agtgacaaat ttatggccat tatttaatca
    16921 atggaagtct ttagtagtca atgagcttat tgatatgaat caatactcag cgcagataga
    16981 attatacgat tacagtggtt atagtctcta tcactgtgag cgaattccgc caataggtga
    17041 tctcttatct actactcaat ggtactggga agcaggtcat ttcaagaaag aacttgggga
    17101 tattatttta gaagaaattc tgagaagcaa tgaaacgatt ctctcaaaag ttatgagtat
    17161 gaatcattca cagacatcat ttgggataag attacttgat cagaatagtt ttctattgaa
    17221 tcaaaatcgt attattcaac aacgtttaat gtgtgagagt aattaccccg agttatttac
    17281 tgatgctgcg atattagcaa gggcagcaca gtaaattatg tatacaattt tgcaaagtat
    17341 tccaaactaa ctttaaccgg ttgtgccttg tacagaattg ttgagcctat aagtgtttcc
    17401 tcaagtcttt accaaggaat aattttccca tcataggcaa aaaattttcc agagtcggat
    17461 aattttagat tcgataatat acgctgcata ccgagtacac tttgttcagg gctaattaat
    17521 gcatctgggc cgcccatatc agttctaacc catccaggat gcagaatcac ggtagtaatt
    17581 ccctgtagtt ttaagtcaac tgaaagactt ttaccaacca tattgacagc tgctttgctt
    17641 gatcggtaaa tataacttcc tcccctttta ttatcatcaa tactgcccat tttactcgtg
    17701 atgatcgcta tgatttttaa ttgactatta cttatttgtg caaggaatgc ttctgccatt
    17761 cttaatggtg ccatagtatt gatttgaaat gctttgtgcc atgcttcgta atcaatctta
    17821 ccaaagccac catcgtcaga tggtgggtag atacctgcat tattgataag cagatcaatg
    17881 gaagtatttc ttaaattgtt ggataaattt gtaatttgat tgtgatcagc gacatccagc
    17941 ggatatatcg ttacgagatc aggaaagcta ttggcaagtt gcctaagatt agaagcattt
    18001 tcgggttgac ggcaacacgc gatgacattc catccgctct gcgcatattg ccgtgcaaat
    18061 tcaaatccta tgccacggtt acttccagtg attaaagtag ttgccataaa taaaatcaat
    18121 ccctttgttt ttttgctatt agagtttagt gttttattga gttattttaa gagtatttca
    18181 taaattggtt agaaataacc aacatta
//
LOCUS       DAOQLJ010000030        17764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1116_length_17764_cov_6.164719, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000030 DAOQLJ010000000
VERSION     DAOQLJ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 17764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17764
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1116_length_17764_cov_6.164719"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..463)
                     /locus_tag="PK522_03745"
     CDS             complement(8..463)
                     /locus_tag="PK522_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40877.1"
                     /translation="MKFERLITAITNLAVVLGLVLVVFELQQNREAIQITHQLSLAGL
                     MSQIHLSVASDHELAELITRAKHDDTKNFSIAETERVNHWVLAILEPRISSYHLRSNE
                     FVVLEDWCNLMRDFTDLYRHPYFGNIVKSELSYADAIAADIENRCAKYR"
     gene            complement(517..1137)
                     /locus_tag="PK522_03750"
     CDS             complement(517..1137)
                     /locus_tag="PK522_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNC40878.1"
                     /translation="MSEYETLITSLALTMGASWASGINLYAVLLVLGIGSATNNIQLP
                     TELSVLENPFVIGAAGIMYTIEFFMDKIPGLDSVWDSIHTFIRIPAGALLAVGTVGDV
                     TPALEIAAGILGGSVAATSHATKAGTRLMLNTSPEPVSNWTASISGDFLVLGGLWTAL
                     NHPIFFLILFIGFIGFSIWFLPKLWRFIKILLQKIGTFFGLRGGQN"
     gene            complement(1134..2678)
                     /locus_tag="PK522_03755"
     CDS             complement(1134..2678)
                     /locus_tag="PK522_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNC40879.1"
                     /translation="MHEETSMHDLKPKGLKKGAVSKWGAITIGLAATAPAYSLTGALG
                     HGAAESGYQLPIVFILSVIPMYFVALSYKHLTTAAPDAGTIFTWGTKAIGPRFGWLGG
                     WALLLASILAGVAATQITVNAIIALMGLKDSPTWLNLVIAILFILSTTYLTALGAKES
                     SRTTMLLTAMQYGVLVVLAAILTLRVLQGDAVATANPFSLEWLNPFAIASFESFLGGF
                     LIALFIFWGFDASLAMSEETAGTSEQIGRSGTTAMMITVITYVVFSITALAYAGVDAQ
                     DPSSLTFEGNIDSIVTALATDTIGEMGAMIAALVIAISAFSATISTIMPAARVALAMA
                     TYRAIPAKFAYVSGVTKTPLVTTWTIGIMSLAIYITLSSISESIVKDSIHSVSIAICT
                     YYTVAALSCVFYFHRTAFNHWYHALSQVILPLIAAVILIAVGIYQAWSMMDPNYGSSG
                     SIAGVGAVFLIGGAALMLGVPLMILWNLREPRFFQGKTLPLERAHMVLDSKDTKKNNS
                     VLKVDK"
     gene            2939..3352
                     /locus_tag="PK522_03760"
     CDS             2939..3352
                     /locus_tag="PK522_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC40880.1"
                     /translation="MESTFLQDNMFFIIAAVISGGMLLFPMISRSAAKEINVQQAVKL
                     INYENALVLDVRDDGEFAEGHLPNAKHIPVERINDRLQELEKYKNNPIVIVFRPGLRA
                     GHAASVLRKNGFDKVYNLNGGIDFWKRDNMPIVKK"
     gene            3377..3634
                     /gene="grxC"
                     /locus_tag="PK522_03765"
     CDS             3377..3634
                     /gene="grxC"
                     /locus_tag="PK522_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HNC40881.1"
                     /translation="MAKVVMYVTGSCPYCVMAEKLLRSKGVEVIDKIRVDLQPDQRIE
                     MMNKTGRRTVPQIYIGDTHVGGYDDLVKLDQQGGVVKLLSA"
     gene            3703..4176
                     /gene="secB"
                     /locus_tag="PK522_03770"
     CDS             3703..4176
                     /gene="secB"
                     /locus_tag="PK522_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HNC40882.1"
                     /translation="MTDQQPIFAIEKIYVKDLSLEIPNAPRVFLDREAPEINIQLNNQ
                     DTHFDGDFYEVQLTITVTARLKENTMFLVEIHQAGIFRIKNIPEKDMGSVLGVVCPNI
                     LFPYLRESVSDTVTRAGFPPVILNPVNFEAIYLQKQQANAAATVAQQSPGTEQIH"
     gene            4191..4667
                     /locus_tag="PK522_03775"
     CDS             4191..4667
                     /locus_tag="PK522_03775"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNC40883.1"
                     /translation="MCNNHRNLTLILRFFWIVLFSLHASISIAADENEFLSISSNSAI
                     MYDAPSLKAEKLFIVNAYFPVQVLVKVEGWTKVKDSSDTIAWIENKFLTERRYVIVTA
                     PLANIHQSADIHSPILFQVQKDVVIELLDSSTTDWVQIRLQNGQMGYIRSNQIWGS"
     gene            4664..5653
                     /locus_tag="PK522_03780"
     CDS             4664..5653
                     /locus_tag="PK522_03780"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNC40884.1"
                     /translation="MKLAILGAGAWGTALAINFSASHPVTLWTRNTDQKAEIERQRVN
                     QRYLPGVALPDTLKLTCELNAAIEAVDLALVTIPIAGLRQLLEQLYKLNKSLPIILCC
                     KGFESPSTRLPHEIVQEVYAGEGSYAILSGPSFAKEVAQQRPTALTLASKDHTFLKII
                     SSQIRTPYLRIYASTDIIGVETGGATKNVIAIAAGISDGLELGSNARAALITRGLAEI
                     TRLGVSLGGHKETFMGLAGLGDLILTCTGNLSRNRQVGLRLAAGMQLADILQDIGHVA
                     EGVHTVQAVLTKGKLHKIEMPITQAVFSVLYEGVSAKEAVRKILEREPKMEVE"
     gene            5968..7200
                     /locus_tag="PK522_03785"
     CDS             5968..7200
                     /locus_tag="PK522_03785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNC40885.1"
                     /translation="MFSLKHSGKKGLLVITLASLFMLFTEVQANPRYAAIVIDATTGT
                     VLHEENADEARYIASLTKMMTLYLLFESIEQKRMNLDTDMRVSAFAASMPPTNIRLQA
                     GNTLSVRDAIKALIVRSANDVAVVVAEALAGSEARFSQLMTNKARQLGMYATTFRNAS
                     GLPNKEQVTSARDMSILSTRLMKDFPQHYHFFSTQSFRHRGITYNSHNRLLRQFDGAD
                     GLKTGFIRASGFHLATSAKRDNHRLVAVVMGGETASARDQRVAELLNRNFDRLANPGS
                     TPAISSAKVLPITEHHRIKTTIPAQKSAALNNQTNESAVKQLSKPQRVTYSPTPRPNK
                     IWGIQLGSYSAHDRAHAHAKAATRWAPGEVAVTMVKISNRKLYRAQLVGLQENQARSA
                     CQNLSRQGIDCMVVRAHG"
     gene            complement(7237..8328)
                     /locus_tag="PK522_03790"
     CDS             complement(7237..8328)
                     /locus_tag="PK522_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNC40886.1"
                     /translation="MYRNIAIDWMRGIVMVLMAIDHASAFFNQGRIANDTIVTHSVGS
                     VFVVEQFFTRWITHLSAPTFVFLTGAAMALSISQRSARGISIQAIDRDLLLRGLFIAL
                     LDLIVLSWFSGSLYLQVLYAIGVSIIAMRYLRYLSSATLLACALALFIGGEAFTNLFW
                     EPHDDVPLWFALTFAPWFTPTFTVLYPAIPWLSIMMLGWVFGDWLTQSPDIRKVSPAR
                     MLFLSGIAAIICFLIVRWLNGYGNMLLYREDFSLIQWLHVSKYPPSLSYISLELGLMA
                     IILAALMWIKPKITVNQNGPILVFGQTALFFYLAHFIVLGLLTIFLEKAGLQQAYLAA
                     LITLLILYPVCRLYRMLKWRYPDSLLRFV"
     gene            complement(8361..10091)
                     /locus_tag="PK522_03795"
     CDS             complement(8361..10091)
                     /locus_tag="PK522_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40887.1"
                     /translation="MHSAFFRSTLQSLFLFPFVFLSGIGWITSSQLLAKEIETSQQND
                     PKPAYQLDTDEKGTVRIQNLGSHRFPVSTHNARAQLLIDQGLNLAYAFNHAEAYRAFQ
                     EAARLEPSLAIAYWGQALVLGPNINAVMEPKDEPRALALIQKAVSLLDSVSTKERALI
                     LALSKRYTGVANERVVNNHAYAAVMRLAYAQFPEDHDIAMLYVESMMNLRPWNYWMPD
                     GQPYEGTAEIVSITEDVLRKNPMHPGALHLYIHLIEPTNAPERAEAAADRLLTLMPDA
                     GHMVHMSSHIYQRVGRYADSMKSNQLAIAADEDYMAQCHAQGIYPMGYYPHNIHFLWF
                     AATLDGQSQPAIDAARKAASKINDETLVVLPAMAIFRVLPYWALTRFGHWQLMLEEPP
                     PPKASIFLTGSWHYARGLAFIATKQLQQAELALESLRSTLAVQDPNWDSPLFSPNTAR
                     SVLAIGPEVLAGEIAAAKGKLDEAIGHLERAVRLEDGLIYTEPAEWHFPPRLALGAIL
                     LEAGRPAEAETVYWEDLRRNRNNGWALYGLSQALRAQSKHGLARMIEDRFKLAWQRSD
                     LALHASRLIR"
     gene            complement(10189..10485)
                     /locus_tag="PK522_03800"
     CDS             complement(10189..10485)
                     /locus_tag="PK522_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40888.1"
                     /translation="MNPKLRNLAVAIVFSSFASMSFAVNHGTSAEVKLYQLSEEVQHG
                     IITNLQGGEAEKIEKSGSDSSPIYEVQVKKSDKSGTEIDRFTIKVDSSGNLISN"
     gene            complement(10664..12721)
                     /locus_tag="PK522_03805"
     CDS             complement(10664..12721)
                     /locus_tag="PK522_03805"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNC40889.1"
                     /translation="MANGLTIAIFSAILALAFSGIWIRRIFSQSPGNERMQEIAAAVQ
                     EGASAYLKRQYMTIGLVGVILFVAISLALSWDTAVGFALGALLSGIAGFMGMTVSVRS
                     NVRTAEAARTGLNEALEIAFRGGAVTGMLVVGLGLLGVAGYCAMLYSGAEGDQAVSDV
                     IQPLVGFAFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNV
                     GDNVGDCAGMAADLFETYAVTIIATMLLGALLFSTNAANAVVYPLMLGAVSIVASIIG
                     CYYVKMRDDGKIMNALYRGLAVAGGIAFFAYLPVTVWFMGSMSLPLGDVVVGGGELVM
                     RLFICAAVGLVLTGLMVIITEYYTSTEYSPVKHIASASTTGHGTNIIAGLGVSMRATA
                     APVLAVCFSILVTYWLAGLYGIAIAATSMLSMTGIIVALDAYGPITDNAGGIAEMSGM
                     PESVRAITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHGLEHAGKILTFDLSN
                     HMVIIGLFIGGMVPYLFGAMSMEAVGRAAGSVVVEVRRQFKEIPGIMEGTAKPDYSRA
                     VDMLTKAAIKEMIVPSLLPVLVPVLVGVILGPQALGGVLMGSIITGLFVAISMTTGGG
                     AWDNAKKYIEDDNFGGKGSEAHKAAVTGDTVGDPYKDTAGPAINPLIKIINIVALLII
                     PLL"
     gene            complement(12848..14107)
                     /locus_tag="PK522_03810"
     CDS             complement(12848..14107)
                     /locus_tag="PK522_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNC40890.1"
                     /translation="MAIKNAFYAQSGGVTAVINASAAGVLETARAHPDKIGKVFAGRN
                     GIIGALTEDLIDTSAESAEAIRALRHTPSGAFGSCRYKLKSLEQNRREYERLIEVFKA
                     HDIGYFFYNGGGDSADTCLKVSQLSGTLGYPLQAIHVPKTVDNDLPITDCCPGFGSVA
                     KYIAVSTREASFDVASMSKTSTKVFVLEVMGRHAGWIAAAGGLASSPDCEIPIVILFP
                     EIAFDKAKFLAKVDSCVKQYGYCSVVVSEGVKGEDGKFLSDQGLRDAFGHAQLGGVAP
                     VVANIIKDGLGLKYHWGVADYLQRAARHVASKSDVEQAYAMGKAAVEYAIAGHNSVMP
                     TIERLSDKPYNWKVGMAELSKVANVEKMMPADFITDDGFGITEKCRTYLSPLIEGEDY
                     PPYKNGLPNYVRLENKAVDKKLEEFKL"
     gene            complement(14170..14823)
                     /gene="adk"
                     /locus_tag="PK522_03815"
     CDS             complement(14170..14823)
                     /gene="adk"
                     /locus_tag="PK522_03815"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNC40891.1"
                     /translation="MRIILLGAPGAGKGTQANFIKEYFGIPQISTGDMLRNAVKAGTP
                     LGVMAKKIMDSGGLVSDEIIINLVKERIAEPDCTKGFLFDGFPRTIPQADALKVAQVA
                     IDYVVEIDVTDSEIIKRMSGRRVHPASGRTYHIVFNPPKVEGQDDISGEPLIQRDDDR
                     EETVMKRLEVYHAQTKPLIQYYSDWSARGDSGAPRYIKIAGTGDVEIIRQNIISQLS"
     gene            15029..16051
                     /gene="recA"
                     /locus_tag="PK522_03820"
     CDS             15029..16051
                     /gene="recA"
                     /locus_tag="PK522_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNC40892.1"
                     /translation="MDDNRNKALEAALSQIEKQFGKGSIMRLGTNDVANDIQVVSTGS
                     LGLDIALGVGGLPRGRIIEIYGPESSGKTTLTLQVIAEMQRAGGTAAFIDAEHALDPQ
                     YAKKIGVNIQELLISQPDNGEQALEIADMLVRSGSVDVVVIDSVAALTPRAEIEGEMG
                     DPQMGLQARLMSQALRKLTANIKRTNTMVIFINQIRMKIGVVFGNPETTTGGNALKFY
                     ASVRLDIRRTGSIKNGEEVIGNETRVKVVKNKVAPPFKQADFDILYGEGISRESEIIE
                     LGVLHKLIEKSGSWYSYKGEKIGQGKDNVRDFLKSHHDLAKELEQKIRTAVGLIPPGA
                     VSESTE"
     gene            16072..16518
                     /gene="recX"
                     /locus_tag="PK522_03825"
     CDS             16072..16518
                     /gene="recX"
                     /locus_tag="PK522_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HNC40893.1"
                     /translation="MAQSNLKTLALQHLARREHSRLELEKKLSKYAQSTEALASTLDS
                     LEQQGSLSANRVVESIKQVRRAKYGSLRIHHELKTKGIAEELIDCAMIDLQQTELDAA
                     RQIWQKKFGVLPENWKTRGKQARFLAGRGFSPEIINQVFASLSEEI"
     gene            16564..>17764
                     /gene="alaS"
                     /locus_tag="PK522_03830"
     CDS             16564..>17764
                     /gene="alaS"
                     /locus_tag="PK522_03830"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNC40894.1"
                     /translation="MKSSEIRQQFLDFFESQGHTVVASSPLVPGNDPTLLFTNAGMVQ
                     FKDVFLGQDKRPYVRAATSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RNAIQYAWEFLTRILQIPPHKLWITVYAEDDEAADIWLNEIGVDPKQFVRIATSDNFW
                     QMGDTGPCGPCSEIFYDHGPEVAGGPPGSTDADGDRYIEIWNLVFMQYNRDASGTLHP
                     LPKPSVDTGMGLERIAAVMQHVHSNYEIDLFQTLIKAAARVTQTTDLTNNSLKVIADH
                     IRACAFLVTDGVIPGNEGRGYVLRRIIRRAIRHGYRLEQKQPFFHLLVEDLATVMGNA
                     YPELVAAKDQVSAVLKQEEERFAETLENGMQVLEAALQTQTKTLAGETVFRLYDTFGF
                     PIDLTADI"
BASE COUNT         5291 a         4163 c         3651 g         4659 t
ORIGIN      
        1 ataggcgtca tcgatactta gcacaccgat tttcaatatc tgctgcgatg gcatctgcgt
       61 aactaagttc tgactttact atattgccga aatagggatg ccggtaaaga tctgtaaaat
      121 cgcgcataag attacaccag tcttccaaga caacaaattc gttacttctg agatggtatg
      181 aactgatgcg tggttcaagg attgccaaaa cccagtgatt tacgcgttca gtctccgcaa
      241 tactgaaatt ctttgtatcg tcatgctttg ctcgagtgat caactctgcc agttcgtgat
      301 ccgatgcaac cgacaagtga atttggctca ttagccccgc tagcgataac tgatgcgtaa
      361 tctgaattgc ttcacggttt tgctgcagct caaatacaac aagcactaag cccaatacca
      421 cagcaagatt tgtaatcgcg gtgatcaatc gttcaaattt catggatgtt acccactatc
      481 aactcgtaag ccttaccgta aaagaaatca agactttcaa ttctgaccgc cccttaaacc
      541 aaagaaggtt cctatcttct gcaacaagat tttaatgaat cgccacaatt taggtaaaaa
      601 ccaaatcgag aaaccaataa aaccaataaa cagaatcaga aaaaagatgg gatgattgag
      661 tgcggtccat aaaccaccca ataccaaaaa atccccactg attgatgcag tccaatttga
      721 tacaggctcg ggagaagtat tgagcatcag ccgcgttcct gctttggttg catggctagt
      781 ggcagcaaca ctgccgccca aaatacctgc agcaatctcc agcgctggag taacgtcacc
      841 gactgtgcca accgccagta atgcaccggc tggaatacgg ataaaggtat gaatcgaatc
      901 ccaaacgcta tccaatccag gtattttgtc catgaagaac tcaattgtat acatgatacc
      961 tgcagcaccg atcacgaaag gattctctaa taccgaaagc tcggttggca actgtatatt
     1021 gttggttgcg ctaccgatgc cgagcactaa caatactgca tacaaattaa tgccgctcgc
     1081 ccatgatgcg cccattgtca gggctaatga tgtaatcagc gtttcgtatt cactcatttg
     1141 tccaccttca atacactatt atttttcttc gtatctttgc tatcgagaac catatgcgct
     1201 ctttcaaggg gcaatgtctt gccctggaaa aatcgcggtt cacgcagatt ccaaagaatc
     1261 atcagcggaa cacctaacat taatgcagca ccgccgatca agaatacagc gcccacccca
     1321 gcaatcgaac cactggaacc ataattggga tccatcatac tccaagcttg gtaaattccc
     1381 accgcaatca gaataaccgc tgcgattaaa ggtaaaatga cttgtgacaa agcgtggtac
     1441 caatgattaa acgccgtacg gtgaaaataa aacacacaag atagcgccgc aaccgtgtag
     1501 taagtgcaga tagcaatgct aacgctatgg atagaatctt tgacgatcga ttcgctaatt
     1561 gaacttaacg tgatatagat cgctaacgac atgatgccta tcgtccaggt cgtcactaaa
     1621 ggcgtttttg tgacgccact gacgtaagca aattttgcag gaatcgcccg gtaagttgcc
     1681 atcgcaagtg caacccgcgc tgcaggcatg atcgtcgaga tcgttgctga gaatgcagaa
     1741 atcgcgataa ccaatgctgc aatcatggcg cccatttcac cgatggtatc cgttgccaac
     1801 gcggtaacaa tcgaatcgat attcccctca aacgttaaac tagatgggtc ctgagcatca
     1861 acgccagcat acgccaacgc ggtaatggaa aataccacat aggtaatgac ggtaatcatc
     1921 attgcggtag tgccactgcg accgatttgc tctgaagttc ctgcggtttc ttctgacatg
     1981 gccagcgaag catcaaatcc ccagaaaatg aaaagtgcga tcagaaatcc acccagaaat
     2041 gactcgaatg aagcaatggc aaacgggttc aaccattcca gggaaaaggg attcgcagta
     2101 gcaaccgcgt caccttgcaa aacacgtagc gtcaatattg ccgcgagcac cactaacaca
     2161 ccatattgca tcgcagtcag caacatcgtc gttctcgatg actctttcgc gcccagcgcc
     2221 gtcaagtaag tggtgctgag aataaacaaa atggcgataa ccagatttaa ccaagtgggg
     2281 ctatctttta aacccatcaa agcgatgatg gcgttgaccg tgatttgagt tgcagcaacc
     2341 cctgcgagaa tactcgccaa taacaacgcc catccgccga gccagccaaa tcgtgggccg
     2401 atcgctttag tgccccaggt aaaaatcgtt ccagcatccg gtgctgcggt cgtgagatgt
     2461 ttataagata acgccacgaa gtacatcgga atcaccgaca aaatgaacac aatcggcaac
     2521 tgataaccac tttctgcagc accatgcccc aatgcaccgg tcagcgaata agctggtgca
     2581 gtagcggcca acccaatcgt tatggcgccc catttcgaaa ccgccccttt tttcaatcct
     2641 ttgggcttta aatcatgcat tgatgtttct tcgtgcattt attctttctc agtgacttgc
     2701 agcgtaaaat aaaaaagttt gatagttgcg acggaaaagt agcaaacaat ttgcatctag
     2761 tcaaaaatgc ggttggttga ttgatagcgg ttgtcgaaca gtagtagttc taatggcgta
     2821 ttgcactaaa acggatttat cttcgtattt aagcatcgtg atttactaat actttgctaa
     2881 aatagccaat caaacaaaac cagcaattag atcaaaactg aactcggaat aaacttatat
     2941 ggaatcaacg tttcttcagg ataatatgtt ttttattatc gctgctgtta tcagcggcgg
     3001 tatgttactt tttcctatga tttctcgtag tgctgctaaa gaaatcaatg tgcagcaagc
     3061 tgtaaaactc atcaattatg aaaatgcctt ggtattagat gttcgcgacg atggagaatt
     3121 tgcggagggg catcttccca atgcaaaaca tatcccggtt gagcgaatta atgatcgctt
     3181 gcaagagctt gaaaaataca aaaataaccc aatcgtgatc gtttttcgtc ctggacttcg
     3241 cgcaggtcat gccgcttcag tattaagaaa aaatggcttc gacaaggttt acaatctaaa
     3301 cggcggaatt gatttctgga aacgggacaa tatgccgatc gtcaaaaagt aacgagtagc
     3361 ggcaattaat cataaaatgg ctaaggtagt aatgtacgtg acggggtctt gcccctactg
     3421 tgtcatggct gagaaactat tacgttctaa aggtgttgag gtcatcgata aaattcgagt
     3481 tgacttacaa cctgatcagc gtattgaaat gatgaataaa actggtcgca gaacagtacc
     3541 tcaaatctat attggagata cccatgtagg gggttatgac gacttggtta agcttgatca
     3601 acaagggggg gttgtcaaat tactatccgc ttagttattt agactcctca cgccgcacta
     3661 aatttattag taaacattgt cctcaaacat aggaaattca atatgaccga tcagcaaccg
     3721 atttttgcga ttgaaaaaat ttatgtcaaa gatttatctt tagaaatccc taacgcaccg
     3781 cgtgtctttc tggatcggga agcaccagaa attaatatac aactcaataa tcaagatacc
     3841 cattttgacg gagattttta tgaagttcag ttaacgatca cggttacagc acgcctcaaa
     3901 gaaaacacca tgtttttagt tgaaattcat caggccggta tttttcgcat aaaaaatatt
     3961 cctgaaaagg atatgggttc agttctaggc gttgtctgtc ccaatatact tttcccttat
     4021 ttaagagaaa gtgtctctga tacagtaacg cgagcaggtt tcccgccggt tatccttaat
     4081 ccggtcaatt ttgaagcgat ctatcttcag aagcagcaag caaatgcggc agcaacagta
     4141 gcacagcaat cccctggaac agaacaaata cactaaaagg aacgatccgg gtgtgtaata
     4201 accatcgtaa tctaacattg attttacgtt ttttttggat agtacttttc agcttacatg
     4261 catccataag cattgctgca gatgagaacg aatttttgtc tatctcgagc aattcagcga
     4321 tcatgtatga tgcaccttct ttgaaggctg aaaaattatt tatcgttaat gcctattttc
     4381 ctgtacaagt tttggtcaaa gtcgaaggat ggacaaaagt caaagatagt agtgacacta
     4441 tcgcttggat tgaaaataag tttctcactg agcgacgcta tgtcatcgta acagcccctc
     4501 tcgccaatat tcaccagtct gctgatattc actcacccat tttgtttcaa gttcagaaag
     4561 atgtcgtgat cgagttactg gattccagca ctacagactg ggtgcagata cgcctccaga
     4621 atggccagat gggttatatt cgatcaaatc aaatctgggg ctcatgaaac ttgcgattct
     4681 gggtgctggc gcctggggta ccgcattagc catcaacttt tctgctagcc acccagttac
     4741 gctatggaca cgaaacacag atcagaaagc cgagatcgaa cgtcagcggg tcaatcaacg
     4801 ttacctccca ggagtagcgc tacctgacac cctaaaatta acatgtgaac ttaatgccgc
     4861 tatcgaagcg gttgatttag ccttagttac tattccgatt gctggattac gccaattact
     4921 cgaacagctt tataagctca acaagtcact gccaatcatt ctgtgctgca aaggttttga
     4981 atcgccatcc acgcgattac cccatgaaat tgtgcaggaa gtttatgccg gtgaaggttc
     5041 atatgcgatt ttgtcagggc caagttttgc taaggaagtt gctcaacagc gccccactgc
     5101 acttacatta gcctctaaag atcatacttt tttaaaaata attagcagtc aaattcgtac
     5161 gccttattta cggatttacg ctagcaccga tatcatcggc gtagaaacag gaggagcgac
     5221 taaaaatgta atcgccatcg ctgcgggtat atcggacgga ttggaattag gaagcaatgc
     5281 ccgtgctgcg ctaattaccc gcggactcgc cgaaataacc cggttaggtg tttctttagg
     5341 tggacacaag gaaacattca tggggctcgc tggattaggt gatttaatcc taacctgcac
     5401 aggtaactta tcacgcaatc gccaggttgg gttaaggtta gcggcaggga tgcaattagc
     5461 ggatattctc caagatattg ggcatgttgc tgaaggcgtt cacactgttc aagccgtgct
     5521 cacaaaagga aaattacata aaattgagat gccaatcaca caagcggtat tcagtgtctt
     5581 atatgaaggt gtgtcagcga aggaagcggt acgaaaaatt ctcgagcggg aacccaaaat
     5641 ggaagttgaa taatgtttca ggcacctctg caacaatcgt ttagtaaaaa ttttgtagct
     5701 atagccccct aaaaatagcc atggctagta gcaaattaaa ataaaatatc gcaaaatacg
     5761 gcgataatgc agaacaaagt tagctaaact tgctattaga aaggatttga aagtttaaat
     5821 cgttgttata aggctgtttt atgccctttt tctagcataa aaatggttac aagcgtgata
     5881 aggtaggatc ggacgcagtt aacttaacgc ttgcttcgcg tcattactag aatttacgaa
     5941 gcatttagga tacggagaat attttttatg ttttctttga aacattcagg gaaaaaagga
     6001 ttactggtta ttacgttagc ttcgctattc atgctattta ccgaagtaca agcgaatcct
     6061 cgttatgcgg ctatcgtcat tgatgcaacg accggcacag tgctccatga agaaaatgcc
     6121 gatgaagcac gctatattgc ttcgttgact aaaatgatga cgctctatct gctcttcgaa
     6181 tcgattgagc aaaagcgaat gaatcttgat actgatatgc gcgtatcagc ctttgctgct
     6241 tccatgccac ctaccaatat acgactacaa gccggcaata ccttgagtgt acgcgacgcc
     6301 atcaaagcgc tgattgttcg ctctgctaat gatgttgcag tcgttgttgc agaagcactc
     6361 gctggtagcg aagctcgatt tagccaattg atgaccaaca aagcgcgcca gcttggtatg
     6421 tatgctacga cattccggaa cgcttcagga ctccctaata aagagcaagt tacttcggcc
     6481 agagatatga gcatactatc aacgcgcctc atgaaagact tccctcagca ctatcatttt
     6541 ttctcgaccc aatcatttcg tcacagaggc attacctaca atagtcataa ccgcctactg
     6601 cgtcagtttg atggggcaga tggattaaaa actggcttta ttcgtgcttc tgggtttcat
     6661 ttagcgactt cggcaaaacg cgataaccat cgacttgttg ctgtcgttat gggcggagaa
     6721 acagcatccg ctcgggatca aagagtcgct gaattgctca atcgaaattt cgatcgacta
     6781 gctaacccag gctctacacc agcgatcagc agcgctaagg ttctaccgat aacagaacat
     6841 caccgcatta aaaccacgat tccggcacaa aaatccgcag cgctaaacaa tcaaaccaat
     6901 gaatcagcag taaaacagct tagtaaacca caacgcgtca cttattctcc aacaccaaga
     6961 cccaataaaa tctggggtat tcaattgggt tcttattccg ctcacgatcg tgcacatgcc
     7021 cacgcaaaag cagctactcg atgggcacct ggtgaagtag ctgttacgat ggtcaagatc
     7081 agtaaccgaa aattataccg cgctcaactg gtcggtttac aagaaaatca agcgcggtca
     7141 gcttgtcaga atctttctcg gcaaggcatt gattgcatgg ttgtccgtgc gcatggttaa
     7201 gttctcagaa aaaagataac gcataaccaa ttttatttaa acaaaacgca acaaactatc
     7261 tggataacgc cacttcagca tacgatataa cctacaaact ggataaagaa ttaataatgt
     7321 aattagtgcg gcaagatagg cttgttgcaa tccagctttc tccaagaaaa tcgttagtag
     7381 accaagcact ataaagtgcg ctaaatagaa gaatagcgcg gtttgcccaa atacaagaat
     7441 aggtccattc tgattgactg taattttcgg ttttatccac attaaagcag ctaatataat
     7501 tgccatcaac ccaagctcca gtgaaatata gcttaggctt ggtggatatt tactgacgtg
     7561 aagccactga atcagtgaga aatcttcacg atacagtagc atgtttccat aaccattcag
     7621 ccagcgcacg atcaagaagc agatgatcgc tgcaatccca gacaaaaata gcatcctagc
     7681 tggagatacc tttcttatat ccggtgattg cgttaaccaa tcaccaaaaa cccaaccaag
     7741 catcatgatt gaaagccacg gtatcgcggg atatagcacc gtgaatgttg gagtgaacca
     7801 aggtgcaaat gttagtgcaa accaaagcgg cacatcatcg tgaggctccc aaaaaagatt
     7861 tgtgaacgct tcgccaccga tgaataaagc caatgcgcaa gctagcaatg ttgcactact
     7921 taagtaacgg agatagcgca tcgcaataat gctgacccca atcgcgtata acacttgtaa
     7981 ataaagtgat ccagaaaacc aagatagtac gatcaaatcg agcaatgcaa taaataaccc
     8041 tcttaacaac aaatcgcgat cgattgcttg gattgatatc ccccttgcgc ttcgttgtga
     8101 aatactcagc gccatcgcag cgcctgttaa aaaaacaaaa gtaggtgcac ttaaatgcgt
     8161 tatccaacgt gtaaagaatt gctctaccac aaaaacagaa ccaacactat gcgtcacgat
     8221 ggtatcgttt gcaattcgcc cctgattaaa aaaagccgat gcgtgatcaa tcgccatcag
     8281 aaccattacg atcccgcgca tccaatcgat tgctatattc cgatacataa tctcgagtta
     8341 actctcaatt caaactaaga ctagcgaatc agacgtgagg cgtgaagtgc caaatctgaa
     8401 cgctgccagg ctaatttgaa tcggtcttca atcattctgg cgagcccgtg ctttgactgt
     8461 gcgcgtagcg cttgacttaa tccatatagc gcccaaccat tgtttcgatt gcggcgtaaa
     8521 tcctcccaat acacggtttc tgcttcggca ggtcgacccg cttctagcag aatagcaccc
     8581 aatgccaatc gaggcggaaa gtgccattca gccggttcag tataaattaa cccatcttcc
     8641 aatcgcaccg cacgttcgag atgtccaatc gcttcatcta attttccttt ggcagcagcg
     8701 atttccccag cgagcacttc aggaccaatc gcaagaactg agcgcgcggt attgggtgaa
     8761 aatagagggc tatcccaatt tggatcttgc actgctaatg tgctacgtaa tgactccaat
     8821 gctagctcag cttgctgaag ttgcttggtt gcaataaaag ccaaaccacg tgcataatgc
     8881 cagcttccgg tcaaaaaaat gctcgccttg ggtggcggtg gctcttctag catcaattgc
     8941 cagtgtccaa aacgagttaa cgcccaatag ggtaatacac gaaatatcgc catcgcagga
     9001 agtacgacca aagtttcatc attaatcttg ctagctgctt tacgtgccgc gtcaatcgct
     9061 ggttgacttt gtccatcgag ggttgcagcg aaccacaaaa aatgaatatt atgcgggtag
     9121 tatcccatcg gatatattcc ctgcgcatgg cattgcgcca tataatcttc atctgctgca
     9181 atcgctaact gattactctt catcgaatct gcgtagcgac cgacccgctg ataaatatga
     9241 gaggacatat gaaccatatg acctgcatcc ggcatcaatg tcaataacct gtcagctgcc
     9301 gcttctgctc gctcaggcgc attcgtcggt tctatcaaat gaatatataa gtgcagagca
     9361 cccggatgca tgggattttt tcttaataca tcctctgtaa tagatacaat ctcggctgta
     9421 ccctcatagg gttggccatc aggcatccaa taattccagg ggcgaagatt catcatcgat
     9481 tcaacgtata gcattgcaat atcgtgatcc tcaggaaatt gcgcataagc taggcgcatc
     9541 actgctgcgt aagcatgatt atttacaact cgctcatttg caacaccggt gtaacgttta
     9601 cttaacgcaa gtatcagtgc gcgttccttg gttgaaactg aatcaagtaa tgacaccgct
     9661 ttttgaatta atgctaaagc ccggggctca tcctttggct ccatgactgc attgatgttg
     9721 ggacctaaca cgagtgcttg tccccagtag gctatagcaa gcgatggttc taatcgagcc
     9781 gcttcctgga aagcccgata agcttcggca tgattgaatg cataggctaa attaagccct
     9841 tgatcgatca ataactgagc tcgtgcatta tgtgtactca ctgggaaacg atgtgagccc
     9901 aggttttgaa ttcgtacagt tcccttttcg tcagtatcta attgatatgc tggttttgga
     9961 tcgttttgct gggaagtttc gatttctttt gccaataatt gactggaagt tatccaacct
    10021 attccactta aaaaaacaaa gggaaataaa aacaaagact gcaacgtaga tcggaagaaa
    10081 gcgctatgca tagcaattcc tttcaactgg aattgattga ttttccaggt ctgatcgaat
    10141 aatcaggcct gggagcaacg aattgattga ttgattgatt actaacgatt aatttgagat
    10201 taaattgccg ctgctatcaa ctttaatcgt aaaccggtca atctcagtac ctgatttgtc
    10261 tgatttcttg acttgcactt cataaatggg agatgagtca gatccacttt tctcaatctt
    10321 ttctgcttcc ccaccttgta aattcgtgat gatgccatgt tgcacttctt ctgaaagctg
    10381 atacagcttc acttcagcac tcgtaccatg attaactgca aaactcatgg acgcaaatga
    10441 tgaaaatacg atagcaactg caaggttacg taattttgga ttcatcttta attctcctat
    10501 ttaattaacg cgtaaatcaa gaaacgaagg aataatgctt tctattaatc ctacactcat
    10561 ttcttatcgt acgcattgcg ttgcagcaat gcaacgaaaa taaagtaaaa agcccatcaa
    10621 gacacgttaa tgggcttttt aaattgctaa aatacaacag atactacaac aatggaataa
    10681 tcagtaaagc aacgatattg ataattttga tcaacgggtt aatcgctggt cctgctgtat
    10741 ctttataagg atcaccaacc gtatcaccag ttacagctgc tttatgcgct tcacttcctt
    10801 tgccgccaaa attatcatct tcgatatatt ttttagcatt atcccatgcg ccaccgcccg
    10861 tggtcataga aattgctacg aataaacctg taataataga ccccatcaat acaccaccga
    10921 gcgcttgagg acctaaaatt acaccaacga gcaccggaac gagtactggt aacagtgaag
    10981 gtacaatcat ttctttgatc gctgctttag tcagcatatc cacagcacgt gaataatctg
    11041 gcttggcagt tccttccatg atacctggaa tttctttaaa ttgacggcgt acctcgacta
    11101 cgaccgatcc tgctgcgcga ccaaccgctt ccatagacat cgcaccaaat aaataaggca
    11161 ccatcccgcc gatgaagagg ccgataatga ccatatgatt ggataaatca aaagtcagta
    11221 tttttccagc atgctccagc ccatgcgtat agtctgcaaa tagcaccaac gcagccaaac
    11281 ccgctgagcc aattgcataa cccttggtaa cagccttcgt tgtattaccc actgcatcca
    11341 gcggatcggt aatggcacga actgattctg gcataccgga catctctgcg ataccgcctg
    11401 cgttatcggt aattggacca taagcatcaa gcgctacaat aataccggtc atcgacaaca
    11461 ttgatgttgc ggcaatcgca attccatata accctgcaag ccagtaagtt actaaaatac
    11521 taaaacatac tgctaataca ggagcagccg ttgcgcgcat tgacacacct aaacccgcga
    11581 tgatgttagt tccatgtcct gtcgttgaag cgcttgcaat atgttttact ggagaatatt
    11641 cagttgaagt gtagtactcg gttatgataa ccatcaggcc agttaagacc aggccgactg
    11701 ctgcacaaat aaataggcgc atcactaact ctccaccacc aaccacaaca tcacccagcg
    11761 gcaaagacat actgcccatg aaccaaacgg ttacaggtaa atatgcaaaa aacgcgatac
    11821 ctcccgcaac cgctaagcca cgatagagtg cattcatgat tttgccatcg tcacgcattt
    11881 tgacgtaata gcaaccaatg attgatgcaa caatcgatac tgcgccaagc atcaggggat
    11941 ataccaccgc atttgctgca ttggtagaaa atagcaatgc gcccagcaac atcgtcgcaa
    12001 taatcgtcac tgcgtaagtt tcaaacaaat cagcggccat ccctgcgcag tcacccacat
    12061 tgtcaccaac gttatctgca ataaccgcag ggttacgtgg atcatcttca ggaataccgg
    12121 cttccacttt accgactaag tctgccccaa cgtctgcacc tttagtaaaa atccctcccc
    12181 ctaaccgtgc aaagatcgat atcaatgaac caccaaaggc aaaaccaacg agtggttgaa
    12241 taacatcgct tacagcttga tcaccctcag ccccgctata aagcattgca caatagccag
    12301 caactccgag caatcccaga cctacgacga gcattcctgt tacagcacca cctcggaaag
    12361 ctatctcaag tgcctcattc agaccagttc ttgctgcttc cgctgttctc acgtttgatc
    12421 gaacagagac agtcattccc ataaagccag caatccctga caaaagagca cccaatgcaa
    12481 atccaactgc ggtatcccag gaaagcgcca ggctaatcgc tacaaataag ataactccga
    12541 ccaagcctat cgtcatatat tgacgcttca gataggccga tgcaccttct tgcactgcgg
    12601 cagcaatttc ttgcatacgt tcatttccag gagattggct gaaaatacgc cgaatccaga
    12661 taccactgaa tgccagggcc aaaatcgcgc tgaatattgc gatcgttaaa ccatttgcca
    12721 tataaaactc caaataaagc cagaccacct gaaaaacatg cgaaattcgg tctggtaatt
    12781 tcgcatcaac tctctaaaat caaacaacaa aaagctattt tattacattc cttaaataag
    12841 gaaaaaatta aagcttgaat tcttccagtt ttttatcaac tgctttattc tcaagccgca
    12901 cgtagtttgg tagtccgttt ttatacggag ggtaatcctc tccttcgatc aatggagaaa
    12961 gataagttct acatttttcc gtgatgccaa agccgtcatc ggtaataaaa tctgctggca
    13021 tcattttttc aacattggct actttcgata attcagccat tcctactttc cagttataag
    13081 gtttatcaga aaggcgctca atggtcggca tcactgaatt atgtccagca attgcatatt
    13141 ctacggctgc ttttcccatc gcataggctt gttcaacatc ggatttggat gcaacatggc
    13201 gcgccgcacg ttgcagatag tcagccaccc cccagtgata tttcaaacca agcccatctt
    13261 tgataatgtt agctaccaca ggtgcaacgc cacctaattg tgcatgcccg aaagcatcac
    13321 ggagcccttg atctgacaag aatttaccat cttctccttt gaccccttca gatacgacaa
    13381 ctgagcaata accatattgc ttaacacagc tatcgacttt tgcgagaaac tttgctttat
    13441 caaaagcaat ttcggggaat aaaattacga ttggaatctc acagtctgga ctcgatgcta
    13501 atccgccagc agcagcaatc caacctgcat gtcgacccat tacttccagc acaaacactt
    13561 tagtagaagt cttcgacata ctcgccacat cgaagctcgc ttctcgggtc gatacggcaa
    13621 tatatttcgc aaccgaacca aacccagggc agcaatccgt tatcggtaaa tcattgtcaa
    13681 cggtcttggg cacatggatc gcttgaaggg gataaccgag tgtacccgac aattgtgaaa
    13741 ctttaagaca cgtatccgca gaatctcctc caccattgta gaagaaataa ccgatatcat
    13801 gcgccttgaa gacttcgatg agccgctcat actcgcgtcg attttgttcc aggcttttca
    13861 atttgtagcg acaagagcca aatgcgcccg atggtgtatg tcgtagtgct ctgatcgctt
    13921 cagcagattc tgcactcgta tcaattaaat cttctgtcaa cgccccaata attccatttc
    13981 ttcctgcaaa tactttacca attttatctg gatgcgcgcg cgctgtctct aaaacacctg
    14041 cggctgaggc attaattact gcggtcacac ctcctgattg tgcataaaat gcatttttta
    14101 ttgccatagc aagacctctc ttcgttttgt tatttattct ttaatcatga atctatttag
    14161 cttttactct tagctaagct gtgaaatgat attttgtcga attatctcaa catctccagt
    14221 accagctatc ttgatatagc ggggcgcacc cgagtcccca cgagctgacc agtccgaata
    14281 gtactgaatg agtggtttcg tttgtgcgtg gtaaacttct aggcgcttca taaccgtttc
    14341 ttctctatca tcatcacgct gaatcagggg ttcaccactg atgtcatcct gtccttccac
    14401 tttgggtgga ttgaatacga tgtgataagt acgaccagaa gcaggatgaa ctcgccgtcc
    14461 ggacattcgc ttaataattt cagaatcggt tacatcgatt tcgacaacat aatcaatagc
    14521 aacctgcgct accttcaacg catctgcttg agggatcgtg cgcggaaaac cgtcaaataa
    14581 aaaccctttc gtgcaatcgg gttcagcaat ccgttctttg acaagattga taataatttc
    14641 atcggacaca agaccgcctg aatccatgat cttcttggcc atgaccccca atggggtgcc
    14701 cgccttaact gcattacgca gcatgtctcc cgtagaaatt tgcggtatac caaaatattc
    14761 tttgataaag tttgcttgcg tgcccttacc cgcacctggg gcacctaata aaatgatacg
    14821 aatgatcttc tccctttttt ctactctttt ttaccggtat ttgcaaaaat aattgttatt
    14881 ttttaatttg aagtttaatt atatctcaag cagaccatcc tcttgaagta tttgctaatt
    14941 tttgcattta tggtattgaa ataaagtaga tatctttctt cctggaatga atatgagata
    15001 atccagagaa caaacattag gaaaaaagat ggacgacaat agaaacaagg cgttagaagc
    15061 ggccctctct caaatcgaaa aacaatttgg gaaaggttcg atcatgcgac tgggcaccaa
    15121 tgatgtagcc aatgatattc aagtcgtatc gactggatcg ctcggtttgg atattgccct
    15181 aggagttggc ggtttaccgc gggggcgtat tatagaaatt tatgggcctg aatcttcggg
    15241 caaaaccaca ctgacgttac aagtcattgc tgaaatgcaa cgcgctggcg gaacagcagc
    15301 ctttatcgat gcagaacatg cactcgatcc tcaatatgct aaaaaaattg gggttaatat
    15361 tcaggaattg ctgatttcgc aaccggataa tggcgaacaa gcattagaaa tcgctgacat
    15421 gttggttcgc tcagggtctg tcgatgttgt tgtgatcgat tcggttgctg cccttacccc
    15481 gcgtgctgaa atcgaaggtg aaatgggaga tccccaaatg ggactacaag ctaggctcat
    15541 gtcccaagcg ctgcgaaaac tgactgccaa tatcaaacgc acgaatacca tggtgatatt
    15601 tatcaaccaa atccgtatga aaattggtgt ggtatttggt aatccagaaa cgacaaccgg
    15661 aggaaatgcg ctcaaattct acgcatccgt tcgcctggat atccgtcgca ctggttcgat
    15721 caaaaatggg gaagaagtca ttggtaacga aacacgcgtt aaagtagtta aaaataaggt
    15781 agcccccccc tttaaacaag ctgacttcga tatcttgtat ggtgagggaa tctcacgtga
    15841 aagcgaaatc atcgagctgg gcgtgttaca taaactcatc gaaaaatcag gctcatggta
    15901 ctcctataaa ggagaaaaaa ttggccaagg gaaagacaat gtacgagatt tcttgaaatc
    15961 acatcatgat ttagccaaag aactcgagca aaaaattcgt acggcagttg gtttaatacc
    16021 ccctggcgcg gtgagtgaat caactgaata ataaacacga cgtgtgtgct tatggcgcaa
    16081 tcaaatctca aaacacttgc cttacaacac ttagcaagga gagagcattc acggctagag
    16141 cttgaaaaga aactatcaaa atacgcgcaa agcactgaag cgctagccag tacactggat
    16201 tcacttgaac agcaaggatc tttgtcagca aaccgagttg tcgagtccat taagcaggtg
    16261 cgcagagcca aatatggatc gttgcgtatc caccatgaat taaaaacgaa aggcattgca
    16321 gaagaactga tcgattgcgc aatgatcgat ctgcaacaaa ccgaattaga cgctgcccgc
    16381 caaatttggc agaaaaaatt tggtgtactt ccagaaaatt ggaaaacgcg cggtaaacaa
    16441 gctcgttttc tagcaggtcg tgggttttca cctgaaatta tcaatcaagt ttttgcgagt
    16501 ctttctgaag aaatttaata cgattctccc tattactatt attgcttgaa aagcgaaatt
    16561 gacatgaaaa gtagtgaaat tagacaacaa tttctagatt tttttgaatc acaaggccat
    16621 accgttgtcg catcgagtcc actcgtgccc ggtaacgatc caaccttgct tttcaccaat
    16681 gctggcatgg ttcaattcaa agatgtcttc ctcggccaag acaagcgacc ttatgtacgc
    16741 gcagcaacgt cacaacgctg tgtacgcgca ggtggtaagc ataatgatct cgaaaatgta
    16801 ggatatacgg cacgacatca tacttttttt gaaatgctag gtaattttag ctttggagat
    16861 tatttcaagc gcaacgccat tcagtatgct tgggaattct tgacgcgcat attacagatt
    16921 ccaccccata aactttggat aactgtctat gctgaagacg atgaagcagc cgacatctgg
    16981 ctcaatgaaa tcggcgtaga tcccaaacaa tttgtacgca ttgcgacctc agataatttc
    17041 tggcaaatgg gtgataccgg gccctgcggt ccttgttctg aaatctttta tgatcatggt
    17101 ccagaagttg ctgggggtcc ccctggatcg actgatgcag atggagaccg gtatattgaa
    17161 atctggaatc tggtattcat gcaatataac cgagatgcga gcggtacatt gcaccctttg
    17221 ccaaaacctt cggtcgatac gggaatggga ctggaacgaa ttgctgcagt catgcagcat
    17281 gtgcatagta attatgaaat tgatttgttt caaactttga tcaaagcagc cgcacgggta
    17341 acacaaacaa ctgatttaac gaataattca ctcaaagtta tcgcagacca tatccgcgct
    17401 tgtgcatttc tcgttacaga tggcgtcatt cctgggaatg aagggcgggg ttatgttctg
    17461 cgccgaatca ttcggcgcgc tatccgacat ggctatcgac tggagcaaaa acaacctttt
    17521 tttcatttac tggtggagga tctcgcaacg gtaatgggta atgcctaccc agaactcgtc
    17581 gctgcgaaag accaagtttc cgcagtactc aaacaagagg aagagcgctt cgcggaaaca
    17641 ctcgagaatg gcatgcaagt cctcgaagcc gcattacaaa ctcaaacaaa aacgttagcg
    17701 ggcgaaacgg ttttccgctt gtacgatacg tttggattcc ctatcgatct taccgcagat
    17761 attg
//
LOCUS       DAOQLJ010000031        17273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1173_length_17273_cov_7.162156, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000031 DAOQLJ010000000
VERSION     DAOQLJ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 17273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17273
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1173_length_17273_cov_7.162156"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..253
                     /locus_tag="PK522_03835"
     tRNA            179..253
                     /locus_tag="PK522_03835"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Val,seq:tac)
     gene            378..1133
                     /locus_tag="PK522_03840"
     CDS             378..1133
                     /locus_tag="PK522_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNC40895.1"
                     /translation="MFKLVNFIIGLSLFLLSHAAFSDLPQVIERIKPSIVGVGTFQKM
                     RSPAINFLGTGFVVGDGSLVITNAHVIPELLDVSKNESLVILTGKSNNPEAKVAQIAK
                     LDKEHDLALLKINGLSLPPLKIGNVDKIKEGQMLAFTGFPIGMVLGFYPVTHRAMIAS
                     ITPIILPVTSTKQLSAEKIKQLNKTAFHVFQLDGTAYPGSSGSPLYDSDSGEVYGVVN
                     LVFVKGKKESALSNPSGISYAIPANFVTELLKY"
     gene            1275..2633
                     /locus_tag="PK522_03845"
     CDS             1275..2633
                     /locus_tag="PK522_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNC40896.1"
                     /translation="MALICTEICLLMIVFYIGAGIRYASWENIEINLFLSELPGALTF
                     TLVTIICMAAMGMYQSDFKLDFESILLRLMPAFALSFAIMTLIFYVLPDLYFGRGLLA
                     IAMVLAFALLLLLRMLFFRLPGVEFFRPRAIVLGTGNSAHELYKLIEEKSFFRNHNIV
                     GYVPFYGEERKIPVTDIIEKSSNLSVLATQKYVSEVIVATQERRGGGFPIQELLECKM
                     NGINVMDIATFYEKERGYIRMDSLQPSWLVFSRGFDQSLIRSLIKRIFDLCASILLLI
                     VTFPIMLITMIIILLEDGKPIFYRQERIGRGGSIFSVIKFRSMRNDAEKGGKPQWSTQ
                     DDPRITRIGKIIRKLRIDELPQIINVLKGEMSFVGPRPERPYFVDMLIAQVPYYNIRH
                     SVKPGITGWAQVRYPYGSSVEDSIEKLQYDLYYVKNHSLFLDLIILIDTIGVVLLGKG
                     GR"
     gene            2652..4724
                     /gene="prsK"
                     /locus_tag="PK522_03850"
     CDS             2652..4724
                     /gene="prsK"
                     /locus_tag="PK522_03850"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HNC40897.1"
                     /translation="MLTLPISALSYAAASLGYFVLFILLVTSWRGKFYGILLAIACIT
                     SSLWAANISAQSYWGYPSIFSIEILEIIKNLSWSLFLIILLGPFQKNESKSSTLKLKS
                     SVIGILLLYLVAIFTAIYSQDTKGVFSGGYQTDYLVSVIMPLIGMILIEQYYRNTPVD
                     QRWGIKFFCLGIGGFFVYDFFLFSDALLLGKVNTDIWDARGWVNLLTVPLVGLSAARN
                     PKWSVGISLSRQILFYSSTLFGAAVYLLAMAGVGYYIRLSGGTWGTVLQLTFLFGAIV
                     LLLVILSSGTTRSWLKVFISKNFYSYNYDYREEWLRFTRTLSEESLELRTRVIKSLAQ
                     LVESPAGILWYKSEQGEFKLTASWNFSLKTQDIADHSNDEFCQFLNQKRWVIDLGEYR
                     LHPQKYSGLALPSWVLANEKLRLIIPLILNNELVGFIALAEPRSIIKLNWEVRDLLWV
                     ASTQATSYLVQYEVTTALSLARQFESFNRMSTFVVHDIKNLIFQLSLLLTNAEKHKEN
                     PEFQKDMMETVSFSVNKMQRLLEKLNSDRETVKTECVFLDQLLRQIVQRKLICEPQPS
                     LDISDEKLMVKADLTRLERVIGHLIQNAIEATSKTGFVKIKLSKNEDKALIKIEDNGH
                     GMSEAFIKNKLFRPFETTKTAGMGIGVFESKEYISELGGQLEVSSREFIGTIFLITLP
                     LMENQKGI"
     gene            4926..6284
                     /gene="prsR"
                     /locus_tag="PK522_03855"
     CDS             4926..6284
                     /gene="prsR"
                     /locus_tag="PK522_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HNC40898.1"
                     /translation="MKTSDDQRKLLVIEDDPGLQKQLRWSFDNCEVFVAGDRESALVQ
                     VRRHEPSVVTMDLGLPPDPDGASEGLATLKQILALAPNTKIIVLTGNQNRENALKAIE
                     LGAYDFHQKPFDSKMLSLIIDRAFYLHALQRENRSLLLSHSDSPISGIVTRDPNMIRM
                     CRNIERVAPSDVTVMLLGESGTGKEVLAKALHQLSTRKEGRFVAINCAAIPESLLESE
                     LFGHEKGAFTGAIKQTLGKIELAQGGTFFLDEVGDLPLSLQAKLLRFLQERVIERIGG
                     REEIPVDVRVVCATHQNLKKLIEEGRFREDLFYRLGEISIKIPPLRERLGDAVLLAHH
                     FRNKFATKEGRHSMSFSQEALSAIESYSWPGNIREMENCIKRAVIMAEGPQIALDDLG
                     IQESAKPQEPLNLREIREKEECKAVVRALAKVDGNIARAAELLGVSRPTLYDLMNRHG
                     LK"
     gene            6347..7924
                     /locus_tag="PK522_03860"
     CDS             6347..7924
                     /locus_tag="PK522_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA ligase (AMP-forming), exosortase A
                     system-associated"
                     /protein_id="HNC40899.1"
                     /translation="MPHLFHELIYKAANCGAKSEALISQQHRLDYLELAEQTQKLATG
                     FIKIGIARGERLAVYLEKRVEGVTALFAASAAGGVFVPINPLLKAEQVSYILSDCNVR
                     ILVTSISRFNLLKSVLTNCHDLHTIIVIDDNVELPSVKGLNLIRWQEVIAEKASCYTH
                     RTIDSDMAAILYTSGSTGKPKGVVLSHRNLVTGAKSVSTYLKNNSDDRILSVLPLSFD
                     YGLSQLTTAFYVGATCVLMNYLLPRDIMDEVIKEDITGLAAVPPLWIQLSQLKWEGLT
                     SLRYITNSGGAMPKATLDQLRGLFPKTSIFLMYGLTEAFRSTYLPPEEINKRPDSIGK
                     AIPNVEVLVLREDGSQCAPGEPGELVHRGPLVSMGYWNDVEKTSACFRQLSPRQSGLT
                     IPEIAVWSGDTVRTDEEGFLYFIGRRDDMIKTSGYRVSPTEIEEIIYSTELVAEVAAV
                     GVPHPVLGQAIVVVVVPRQDVNLNADVLLSACKQALPAYMLPALVDIRKNSLLRNPNG
                     KIDRKSLANELQHSFIN"
     gene            7938..9173
                     /locus_tag="PK522_03865"
     CDS             7938..9173
                     /locus_tag="PK522_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase, exosortase A
                     system-associated"
                     /protein_id="HNC40900.1"
                     /translation="MTNNRPKHASFDQFPIRDHCIQIGGMSLNRLTQRVGSTPYFAYD
                     RKLITERILFLRQCLPNKIKLHYAMKANPMPAVVQHIAGLVDGIDVASLGEIKVALDT
                     GMLPSKISFAGPGKNEHELSCAIAAGIVINIESELELERIVALAERLGITPKVAVRIN
                     PDFELKSSGMKMGGGPKQFGIDAERAPIVLRRIVEFGIVFEGLHIFSGSQNLKVEAIQ
                     DAHEKTLQLAVRLAKESSVSIPSLNMGGGFGIPYFPGESPLNLVAVGENLQHLLNKIE
                     SELLDTQFVIELGRYIVAEAGIYVCRVLDKKNSRGQLFLITDGGMHHHLAASGNFGQV
                     IRKNYPVTVGTKAETAETEVVSVVGPLCTPLDLLADQMELPKAEIGDLIVVFQSGAYG
                     LTASPISFLSHPIPLEVLV"
     gene            9235..11082
                     /locus_tag="PK522_03870"
     CDS             9235..11082
                     /locus_tag="PK522_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNC40901.1"
                     /translation="MSGVCGWVGYGQSVVENQQISESMAQVLTRFDHSRSQIYVDENS
                     SLAIAASEQQLHYSHKNDIIVGIWGHAKFNNDELNQLILSQGIAGVLLDQWVNNGPSV
                     IESLLGEFICCLVNAKSHEVFIAVDRMGTRPLYYQILENGLLFGTSLDAMMRHPLAKD
                     EISNQGIYDYIYFHMVPGPNTIYREPRRLLPGEYIYYKHGTLTVERYWNAQFRENEAQ
                     SFSELKQSFISLLHTSMEDAIAGYQHVGAFLSGGTDSSTIAGMLQEVTGKPAKTFSIG
                     FDAPGYDEMDYARIAARHFSTNHHEYYVTPEDVASAIPQVAAIFDQPFGNASAIPSFY
                     CAQMAKKDGIDLLLGGDGGDELFGGNVRYAKQYVFSLYERIPNVLREKIITPFALSTL
                     AGSNISLFRKIHSYISQASIPMPHRMETYNLLIRYGYQDVFTEDFLSNIDINEPASQI
                     NQTYHKAETTTLINRMLAYDWKYTLTDNDLPKVTKACELAAVDVAYPFLNSEMVSFSE
                     RLRPELKLKKTKLRYFFKEALRDFLPKEIITKQKHGFGLPFGIWLQENKLLQAQAADC
                     LTDLKKRHIFRVNFIDKILSQYLHQHASYHGTMVWLLMMLECWYKQRSR"
     gene            11472..12263
                     /locus_tag="PK522_03875"
     CDS             11472..12263
                     /locus_tag="PK522_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM/exosortase system-associated
                     acyltransferase"
                     /protein_id="HNC40902.1"
                     /translation="MSDIADAFHEYFEIVVADTLELKKEVYRLRYRIMCLNTGIFDTT
                     LYPDELEKDEFDDHSIHILLRHKPSNAFMGTARLILSDPSPLANRAFPVELHTQFYPA
                     FSNMNIIREHTVEISRFAIHSDFFRRKDDFRFSVDKAASQGIRKNERRRFPHPMLGLA
                     VGIIQLCAKHDFFFWFSAMDPALNRLLGFYGMQLNPIGPIVDYYGPRRPYHVCVIDVL
                     ERMYVNYPEVWELVTDNGQIWPSDLQSVRERNQHILLASNEMVTC"
     gene            12279..12737
                     /locus_tag="PK522_03880"
     CDS             12279..12737
                     /locus_tag="PK522_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40903.1"
                     /translation="MVARNRFNYCYRIVSLFFFVFGVSYFATHTYANDSVEAVIHPSV
                     ELKSYSRNSLRAAFGMRLKTWPNGMPVRVFVMPDDAVLHTIFTKEKLNIFPYQLRAGW
                     DKLIFSGTGQAPFLVRTEEEMRERISSTPGAIGYLKRANIDESIHVLNIE"
     gene            12763..14007
                     /locus_tag="PK522_03885"
     CDS             12763..14007
                     /locus_tag="PK522_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40904.1"
                     /translation="MMCRLASVVLLLIIMMQEGIAQDVKGIRVHGFLSQGYLKSTGNN
                     DVFGNSSGNGSFDFREIGANASYRPLPNLQFSGQLLSRSAGEDSKGGIRIDYGFVDYT
                     AISTESMDFGIRLGRTKNPLGFYNDTRDVPFTRPSILLPQSIYFDRTRNLGLASDGAQ
                     IYGESRNSWGDLNAQFGVVFPQAGDRSTEFAILQADRPGGITPHLSYIGRLIYEYDGG
                     RVRLALSGAQVNTGYDAAQVDPFSGLGSFQFTPIIFSAQYNTERWSLTSEYAIRHIER
                     YDFANRSARDLNLTGESYYFQGIYRFSPNWEGVLRYDAYYADRSDRDGKDFAKFIQIQ
                     APDFQDNFPSYSRFAKDITVGLRWNVTPSIMLRAEYHYINGTGWISSLDNRAPNTTLQ
                     RFLPAITDQHWNLFAFQASYRF"
     gene            14019..16958
                     /locus_tag="PK522_03890"
     CDS             14019..16958
                     /locus_tag="PK522_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC40905.1"
                     /translation="MKLSHLVSLTRLDREIHKTLSKFFSLKWKISLLSSLILLIVVTM
                     FSVINYRSLIDNVDYQSSLQYQRYVTEVENLIEQTSNNSRQLIRLIPFLKEMDTALVL
                     QDSAQITQAFEQHWSLFQLHNGIETIRFYDQSDQLLVGWDNFEEDSQRDALILEQVHD
                     VNSQELPASLLICIEMCLQYTIEPLLIDGVRAGAMVIGASLADVFLGFKRTFGSDIGL
                     LLKMKADGVDIQQNDVEFSNWGVRLAALTNREKNLEILHQIAQSHPTIQDLRVGIHTE
                     WDNRHHRIKLLSLRGVNVLDRAYLVVLTDITPTILSIRDSTWRIIIIGLVGLLVSELI
                     LFAILSLPLTRLKHIVFTLPLLAGGSFGIFRATLASVSRKRWLKDEVDLLYEAAVALS
                     HKLEKLENKVAYRTKLLVQQRDELSKEKDFIAHLLDTAQVIVLTQNVSGKILMLNAYG
                     ELLMRYSKEEIQTKYFLEVLAPDNDQQYNLVNYLEEIRRGHRDQLRHEAMTLCNDGSK
                     RHIVWLHSRLTWQSETDPSVLSVGLDITEYKRVEGHLAWLADHDPLTNLFNRRRFSEE
                     LEQMLSWAERYQHPGALLFFDLDRFKYINDTSGHQAGDALLKMVAEMLSRTIRTADVT
                     GRLGGDEFAVILPEISAGGAIEVAKKILLRLSETQLNVNGRTYKIAASVGIALFPEHG
                     KNVHDLLAAADLAMYQAKEIGRGTWHLFSNDDKTRERLYTLVHWKEKIEYANLYDHYL
                     LYFQPIMSLHDRAITHYEVLLRMHDNDGTIHSPAAFIAAAEHTGLIHAIDHMVLRKAI
                     TQVAKINSKSTKPIRFSINLSAHAFNDPELLPVIRHELTSSGVDPTLLFFEITETAAL
                     DNLPGTRNLLREIKKLGCGFVLDDFGVGFSSFYYLRELPVDAVKIDGSFIRNLANNPD
                     DQILVRALCSVAKGFGKKITAEFVESEEILMLLGKMDVDYAQGYHVGKPAAAEDIFRL
                     FEENEIS"
     gene            complement(16968..>17273)
                     /locus_tag="PK522_03895"
     CDS             complement(16968..>17273)
                     /locus_tag="PK522_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNC40906.1"
                     /translation="DAWFGRSIGPRQHLQISQMRALETGRYMLRATNTGVTAIINERG
                     QIVQELEMFVTQGLHGTAQGFKGATPYVQYGNTLVLTIIGLCLSLGGFIAFFRKEND"
BASE COUNT         4981 a         3051 c         3803 g         5438 t
ORIGIN      
        1 attgatcttg ctatataaga tgaaaaacac tgagaatagt tcataggcat ttttttttaa
       61 taaaacatta ttttgtaatt caattccata ataataatta gattaatctc ttataaaatt
      121 gaccaagatc gttacgatat gtttaaattc agaaagcata agattatacg atattattag
      181 gcggttaact cagtggtaga gtgtcaccat tacttggtga aagtcattgg ttcgattcca
      241 gtgccgccta ccaattaggg attggaattg atttagcact ggacgaaaat acttacatcc
      301 catttttgcg aaattcagct tatcgataat tgtcaattgt ctttattgat acctaacttt
      361 ttaagacacc ggtgcaaatg tttaagcttg taaattttat tatcggatta agcctgtttt
      421 tattatctca tgcggccttt tctgatctac ctcaagtaat tgaaaggatt aagccctcaa
      481 tcgtaggggt cggtacgttc cagaaaatgc gttcacctgc tatcaatttc ttgggaactg
      541 gttttgtagt tggggacggt tctcttgtta taacaaatgc gcatgtgatt cctgaattac
      601 tcgatgtttc aaaaaatgaa tcactcgtga ttttgactgg gaaaagtaat aatcctgagg
      661 ctaaggttgc tcaaatcgct aaattggata aagaacatga tttagcgctg cttaaaataa
      721 atggcttatc gctgccccca ttaaaaattg gtaatgttga taaaattaaa gaggggcaga
      781 tgcttgcttt tacagggttc ccaatcggga tggttttagg gttctatcct gttacacata
      841 gagcgatgat agcttcaatt acaccaatta ttttacctgt tacgagtact aaacaattga
      901 gtgccgaaaa aataaaacag ttaaataaaa ctgcttttca tgtttttcag ctagatggca
      961 ctgcttaccc cggcagtagt ggaagtccac tctatgattc tgattccggg gaagtttatg
     1021 gggttgttaa tttggttttt gtaaaaggaa aaaaagaatc ggcactcagt aatcccagtg
     1081 ggattagcta tgctattcct gctaactttg ttacagaact gttaaagtat tagtaatggt
     1141 aatctcatcc tatggaatta taagtaagta gggattatca aatgaattct ctattattat
     1201 ttctataaaa ttttttcttt attaggtgag gaaatttgat tcgcgtatat aaccattaca
     1261 tttctcgatt aactttggca cttatttgta ccgagatttg tttattgatg attgttttct
     1321 atattggtgc tggaattcgc tatgcttctt gggaaaatat cgaaatcaat ctttttttgt
     1381 ctgaattgcc tggcgcgctg acctttacgt tagtaactat catctgtatg gcggctatgg
     1441 gtatgtatca atcagatttt aaattagatt ttgagtcaat attattgcgg ttgatgcctg
     1501 cgttcgcatt gagctttgca ataatgactt tgatttttta tgttttacct gatctttatt
     1561 ttgggcgtgg tttgcttgct attgcgatgg ttcttgcttt tgcattgcta ttgctgttaa
     1621 gaatgttatt ttttcgattg cctggcgtgg agttctttcg cccgagagca atcgtacttg
     1681 gaacgggaaa tagtgcccat gaactctata aactgatcga agaaaaatct ttctttcgta
     1741 atcataatat tgttggatat gttccttttt atggggaaga gcgaaaaatt ccggttacgg
     1801 atattattga gaaaagtagt aatctaagtg ttttggcaac ccagaaatat gtaagtgaag
     1861 tgatcgttgc aactcaggag cggagaggag gagggtttcc aatccaagaa ttattagaat
     1921 gtaaaatgaa tggaatcaat gtgatggata tagctacctt ttatgagaaa gaaagaggtt
     1981 atattcgaat ggattcactt cagccaagtt ggttagtttt tagtagaggg tttgatcaaa
     2041 gcctgattcg atcattaata aagcgtattt ttgatttatg tgcaagcata ctgctattga
     2101 ttgttacgtt tccaatcatg ctgatcacga tgatcatcat tttattagaa gatggaaaac
     2161 cgattttcta ccgtcaggag cggattgggc gtggaggaag cattttttca gtcattaaat
     2221 ttagaagtat gagaaatgat gcggaaaaag gagggaaacc ccaatggtct actcaggatg
     2281 atcctcgtat tactcggatt ggaaaaataa ttcgtaagct gcgaattgat gagcttcctc
     2341 aaatcattaa tgtgttaaag ggtgaaatga gttttgttgg tccgcgccct gaaagaccct
     2401 attttgtaga tatgcttatc gcacaagtac cttactacaa tattcggcat agcgttaaac
     2461 ccggaataac gggttgggct caggttcgtt atccctatgg ctcatcagta gaagattcca
     2521 tcgaaaaatt acaatacgat ctatattatg tgaaaaacca tagcttgttc cttgatctca
     2581 ttattctgat cgatacgata ggcgttgtgc tattgggtaa aggtggtagg taggaataat
     2641 cagttgatca tatgcttacg ctacccatta gtgcattgag ttatgccgct gcatcgctag
     2701 gatattttgt tcttttcatt ttgctggtta caagttggcg tggaaagttt tacgggatat
     2761 tgttggcaat tgcgtgcata acgtcatcac tctgggctgc taatatctca gcccagtctt
     2821 attggggtta tccttccatt ttctcgattg agatactaga aataattaaa aacttaagct
     2881 ggtcattgtt tttaattatt ctacttggtc cttttcaaaa aaatgaaagt aaatcaagta
     2941 cattaaaact taaatcatcc gtaatcggga tcttactact ctacttggtt gctattttta
     3001 ctgccattta ctcccaggat acgaagggag tattctcagg tggatatcaa acagattatt
     3061 tagtgagtgt catcatgccg ctgataggta tgattttaat cgaacagtat tatcgaaata
     3121 cccctgtaga tcagcgctgg ggaatcaaat ttttttgctt aggaatcggt ggtttttttg
     3181 tatacgattt ttttcttttc agtgatgctt tattgttagg taaagtaaat actgatattt
     3241 gggatgctcg tggctgggtc aacttgctca ctgtgccatt agttgggtta tctgcggcaa
     3301 ggaatccgaa gtggtcagtc ggcatttctc tatctcgcca aattcttttt tactcatcaa
     3361 ccttatttgg tgcggctgta tatttactcg caatggcggg agttggttat tacataagac
     3421 tctccggagg aacgtgggga actgttctgc agcttacttt tttgtttggt gcaatagtat
     3481 tacttttggt tattttatct tcaggaacga cgcgttcctg gcttaaagta tttatcagta
     3541 aaaattttta tagctacaat tatgattatc gtgaggagtg gttgcgtttt acacgcacct
     3601 tatcggagga gtcgttagaa ttaagaacta gggtaattaa atcactcgcg caattagttg
     3661 agagccctgc tggaatactt tggtataaat cagagcaagg agaatttaag ttaacagcga
     3721 gttggaattt ttcacttaaa actcaagata ttgctgatca cagtaatgat gaattttgcc
     3781 aatttcttaa ccaaaaaaga tgggttatcg acttaggcga gtatcgatta catccacaaa
     3841 aatattcagg cttggcgctg ccatcatggg ttttagcaaa tgagaaactt cgattaatta
     3901 ttccccttat tctcaataat gagttggttg ggttcattgc gctggctgaa ccgagaagta
     3961 taattaaatt gaattgggaa gttagggatc tgctatgggt tgctagtaca caagcaacca
     4021 gttatttagt tcaatatgaa gttacaacgg cgttgtcctt agcgcgacaa tttgaatctt
     4081 ttaatcgtat gtctactttt gtagttcatg atataaaaaa tttaattttc cagttatcac
     4141 ttttactcac gaatgctgag aagcataaag agaatcccga atttcagaaa gatatgatgg
     4201 aaacagtgag tttctcagta aataaaatgc aacgcttgtt ggaaaaatta aatagcgatc
     4261 gtgaaacagt aaaaacagaa tgcgtgtttc tggatcaatt gctgagacaa atagtacagc
     4321 gaaaattaat ttgtgagcca caaccttcgt tagatatatc ggatgaaaag cttatggtaa
     4381 aagctgatct aacacggctt gagcgagtta ttgggcatct aattcaaaat gcaatcgaag
     4441 caacatctaa gactgggttt gtaaaaatta aactttctaa aaacgaagac aaagcgctaa
     4501 ttaaaattga agacaacggg catggtatga gtgaagcgtt tatcaaaaat aaactatttc
     4561 gaccttttga aacaactaaa acagcgggta tgggaattgg tgtttttgag agcaaagagt
     4621 atattagtga gcttggaggg cagttggaag tttctagtcg tgaatttatt ggaaccattt
     4681 ttttgattac tttaccgctt atggaaaatc aaaagggaat ttgatctaat tttttgcttt
     4741 ttatcagcta attactcgat agcgccattt gctaacatgg acagatgtcc acgaggtatt
     4801 ctggtttgct gagctgaaac gtataatatt tcttacgaat aatcgcttgc ctcagcttat
     4861 cgctaatatt cccgtataag ttattttggt aaaaggttat agagtaaaga ggctatagag
     4921 agaacatgaa aacaagtgat gaccaaagaa agttacttgt gattgaagat gatccaggtc
     4981 ttcagaagca attacgttgg agttttgata actgcgaagt ttttgtggct ggtgatagag
     5041 aaagtgcact cgtacaagtt cgacgtcatg agccttctgt ggttactatg gatttagggt
     5101 tgccacctga tcctgatggg gcttcagaag gacttgctac tctaaagcaa atactcgcat
     5161 tagcgcctaa tactaagata attgtattaa cgggaaacca gaatcgagag aacgcactta
     5221 aggcgattga actaggtgcc tatgatttcc atcagaaacc ttttgattca aaaatgctaa
     5281 gcttaattat cgatagggct ttctacttgc atgcgcttca acgtgagaat cgttcactat
     5341 tattgtctca ttcagattca cctatctcag gaattgtaac tcgtgatcct aatatgatca
     5401 gaatgtgtcg caatatcgag agggttgcgc cttctgatgt cacagtcatg ttactcggag
     5461 agagtggtac aggtaaagaa gttctagcta aagcgctgca tcaattgagc actagaaaag
     5521 aagggcgttt tgttgcgatt aattgcgctg ctattcctga aagcttatta gaaagtgagc
     5581 tgttcggtca tgaaaagggt gcttttaccg gtgcaattaa gcagacatta ggtaaaattg
     5641 aactggcaca aggcggtacc ttcttccttg atgaagtggg tgatttaccc ctatcattac
     5701 aagcgaaatt gctgagattt ctccaagaaa gagttatcga acgaattggt gggcgcgaag
     5761 aaataccagt cgatgttcga gttgtttgtg ctacccatca gaatcttaag aaattgatcg
     5821 aagaagggcg tttccgagag gatttgttct atcgattggg cgagatttcc atcaaaattc
     5881 cacctttacg cgagaggtta ggagatgcag tactgctggc tcatcatttt cgaaataaat
     5941 ttgcgactaa agagggtcgt cattcaatga gtttttccca agaagcatta tcagctatag
     6001 agtcttacag ttggccagga aacattcgtg aaatggaaaa ctgtattaaa cgcgctgtta
     6061 ttatggcaga gggcccacaa attgcattgg atgatctagg aattcaggag tctgctaaac
     6121 ctcaagagcc gcttaatttg cgtgagattc gtgaaaaaga agaatgtaag gcggttgtga
     6181 gagcactagc gaaagtggat ggaaatattg ctagggcagc tgagttatta ggcgtcagtc
     6241 gacctacctt gtatgactta atgaatcgac atggacttaa atagcaattc tagaaagacg
     6301 agaatctctt gagatctatt catttaataa tttattatat tcgcttatgc cgcatttatt
     6361 ccatgaactg atttataaag ccgctaactg cggtgctaaa tcggaagcgc ttattagtca
     6421 acagcatcgg ctggactatt tagaattagc agagcaaact cagaaactag caactgggtt
     6481 tattaaaatt gggattgcac gtggtgaacg gctggctgta tatcttgaga aaagagttga
     6541 aggagttacc gcattattcg cagcttctgc agcaggtggt gtattcgttc caatcaatcc
     6601 tctgctcaag gcagagcaag ttagctatat ccttagtgat tgtaatgtaa ggattctagt
     6661 tacatcaata agtcgtttta atttgctgaa gtcagtttta acaaattgcc atgatttgca
     6721 tacgattatc gttatagacg ataacgtgga gttacctagc gtcaaggggc taaaccttat
     6781 tcgttggcaa gaagttatag ctgaaaaagc atcttgctat acacatcgca cgatcgatag
     6841 tgacatggct gctattctct atacgtcagg aagtacaggg aagccaaaag gtgtagttct
     6901 ctctcaccgt aatcttgtca cgggtgcaaa aagtgtttct acttatttaa aaaataactc
     6961 agatgaccgg attttatcag tattaccgct aagctttgat tatggattaa gccagttaac
     7021 aactgcattt tacgtgggtg cgacttgtgt attgatgaat tacttgctac ctcgtgacat
     7081 tatggatgaa gtaatcaaag aggatattac tggcctggca gcagttcccc ccctgtggat
     7141 acagctttcc caactaaaat gggaaggctt gacatcactg cgttatatta ccaactcggg
     7201 tggcgcaatg cctaaagcga ctttagatca acttcgtggc ctttttccaa aaacaagtat
     7261 tttcctgatg tatggcttaa cagaagcatt tcgctcgact tatttgcccc ctgaagagat
     7321 taataaacgt cctgattcga tcgggaaggc tatacccaat gtagaggttt tagtattacg
     7381 agaagatgga tcacaatgtg cgccgggtga accgggtgaa cttgtacatc gaggtccgct
     7441 tgtttcgatg ggctattgga atgatgtgga aaagacatcg gcttgtttta gacaattgtc
     7501 gcctcgtcaa agtggtttaa ctattcctga aattgctgtg tggtcaggag acacagtgcg
     7561 aacggatgag gaagggtttt tgtatttcat tgggcgtcgc gatgatatga ttaaaacttc
     7621 gggttatcgc gttagtccta cggaaataga ggaaattatt tattcaaccg aattagttgc
     7681 tgaggttgct gcggtaggcg tcccacatcc agtattgggg caggccattg tggtagtagt
     7741 cgttccaagg caagacgtaa atcttaatgc tgatgtttta ttgtccgctt gcaagcaagc
     7801 gctccctgcc tatatgttac ctgcgctagt cgatataaga aaaaatagct tacttcgtaa
     7861 tcctaatggg aagattgacc gtaaaagtct agctaatgaa ctccaacatt catttataaa
     7921 ttaagtatgg actagcgatg acaaataatc gacctaagca tgcgtctttc gatcagtttc
     7981 cgatacgcga tcattgcatt cagatcggag gtatgtctct taatcgttta actcagcgtg
     8041 ttgggagtac tccttatttt gcttatgacc ggaaactgat tactgagcgt attttgtttt
     8101 tgcgccagtg cctacccaat aaaataaaat tacattatgc aatgaaagcg aatccaatgc
     8161 cggcggtcgt tcagcatatt gcaggtttgg ttgatggtat tgatgtcgct tcattgggag
     8221 agattaaggt agcactagat acagggatgt taccgagtaa aataagtttt gctgggcctg
     8281 gaaaaaatga acatgagctc tcctgcgcaa ttgctgctgg tattgtgatt aatattgaat
     8341 cggagcttga gttagagcga attgtagctc ttgctgagcg cttaggaata acgcctaaag
     8401 tagccgttcg cattaatcct gatttcgaac tgaaatcctc aggaatgaaa atgggaggag
     8461 ggcctaaaca gtttggaatt gatgctgaac gtgccccaat tgtacttcgt cgaattgttg
     8521 aattcggtat agtatttgaa gggcttcata tttttagcgg ttcacagaat cttaaagtag
     8581 aagcaatcca agatgcgcat gaaaaaacac ttcaactagc agttaggctt gcgaaagaat
     8641 cttcagtttc tatcccttct cttaacatgg gtggagggtt tggtatacct tattttcctg
     8701 gggaatcgcc gttgaacctt gttgctgtgg gtgagaatct acagcatttg ctgaacaaaa
     8761 tagagtcaga acttttagat acacagtttg tgattgagct gggtcgatat attgttgccg
     8821 aggcagggat atatgtatgt cgggtgttag ataaaaaaaa ttcgagggga caattatttt
     8881 taattaccga tggtggcatg catcatcatc tagcagcttc aggtaatttt ggtcaagtta
     8941 ttcgaaaaaa ttacccagtc acagttggaa ctaaggcgga aaccgcggag actgaggtgg
     9001 tatcagttgt tggtccatta tgtactccgc ttgatttgtt ggcagatcaa atggaattac
     9061 ccaaagcaga gattggtgat ctgatagtgg tatttcaatc aggtgcttat ggtttgacag
     9121 cgagccctat atcatttctc agtcacccca tacccttaga ggttctggtt tagtctactt
     9181 acttctctag ataagctcag gcttaaattt ctataactat aaaaaggaat cgatatgagt
     9241 ggagtgtgtg gttgggttgg ttatgggcaa tcagttgttg agaatcaaca gattagtgaa
     9301 tcgatggctc aagtacttac ccggtttgat cacagtcgct cgcagattta tgtcgatgag
     9361 aactcatctc ttgcaatcgc agcaagcgaa caacaacttc attactccca taaaaatgac
     9421 attatcgttg gaatatgggg acatgcaaaa tttaataatg atgagctgaa tcaactcatt
     9481 ctctctcagg gaatcgctgg tgttttactt gatcagtggg tgaacaatgg cccaagtgtt
     9541 attgaaagcc tactcgggga atttatctgt tgtcttgtca atgctaaatc ccatgaagta
     9601 tttatagccg ttgatcgaat gggaactcgc ccactttact atcaaattct agaaaatggt
     9661 ctactatttg gtacttcgct tgatgcgatg atgaggcacc cactcgcaaa agacgaaatt
     9721 agcaatcagg gtatttatga ctatatttat tttcatatgg tgcccggtcc taatacaatt
     9781 taccgtgaac cgagacgctt acttcctggt gaatatattt actataagca tgggacgctt
     9841 acggttgaac gttattggaa tgcgcaattt agagaaaatg aggcacagtc gtttagtgaa
     9901 ttaaaacaaa gctttatttc gctgctgcat acgagtatgg aggatgcaat tgcaggttac
     9961 cagcatgtgg gcgcattcct tagtggtgga acggatagtt cgacaatcgc aggtatgctc
    10021 caagaagtta cgggaaaacc tgctaaaaca ttttctatcg gctttgatgc cccgggttat
    10081 gatgaaatgg attatgcacg cattgctgcc cgacattttt cgacaaatca tcatgaatat
    10141 tatgtgacac cagaagatgt tgcatcggct ataccacagg ttgcagctat ttttgatcaa
    10201 ccttttggga atgcatctgc tatacccagt ttttattgtg ctcagatggc aaagaaagat
    10261 gggattgatc tgttattagg tggtgatggt ggtgatgagc tttttggggg taatgttcgc
    10321 tatgctaagc aatacgtttt ttctttatat gaaagaattc ctaacgttct tcgggaaaaa
    10381 ataattacac catttgcgct atcaaccctt gctggatcga atataagctt atttcggaaa
    10441 attcatagct acatttctca agcatccata ccgatgccac atcgtatgga gacctataat
    10501 ttactcattc gttatggtta tcaagatgtt tttaccgaag attttttatc aaatattgat
    10561 ataaacgagc ctgcctcaca aattaatcaa acctatcata aagctgagac aaccacactt
    10621 atcaatcgta tgctagctta tgactggaaa tatacattaa cggataatga tttaccaaaa
    10681 gtcactaaag cttgtgaatt agcggcagta gatgttgcct atcctttttt gaatagcgaa
    10741 atggtttctt tttctgagag acttagacct gaacttaagt taaagaaaac gaaactacgt
    10801 tatttcttta aggaagcact acgtgatttc cttccgaaag agattattac taaacaaaag
    10861 catggattcg gattgccatt tggaatttgg ctccaagaaa ataaattact tcaagcgcaa
    10921 gctgcagact gtttgacaga tctaaaaaaa cgtcatattt tccgcgtcaa ttttatagac
    10981 aaaattttaa gtcagtatct gcatcagcat gcgagttatc acggaacaat ggtttggttg
    11041 cttatgatgt tagaatgctg gtataagcaa cgttctagat aatcgtctac ttctgcttta
    11101 ttctacctat taatctgcgc atgtatctat caggctttac atcccagcgc gactttataa
    11161 tctagcgtaa ttcacttctg taattccata tcgataaaac ttctttgcgt ataaagtatg
    11221 tacgtttgga ggttttcatg atatttttca actaatcaaa gacatatact aacaggttag
    11281 tatgttatgt aattattgtt atttaatcta aagaattatt tagtttaaga aggcagaagc
    11341 tcactttgct aataataatt aattgaaata aaggtaaacc tagaatcaat tccaaacttt
    11401 aggtctacta atgattagta caatctagtt tctttagatt acattaaccc attaatttgg
    11461 gaaggattat catgagcgat attgccgacg cttttcatga atattttgaa attgtggtag
    11521 cggataccct agaattaaaa aaagaagtgt atcgtctgcg ttaccgaatc atgtgtttaa
    11581 atacgggaat ttttgatact acgctatatc cagatgagct agaaaaagat gaattcgatg
    11641 accattcaat tcatattttg cttcgtcata aaccttctaa cgcttttatg ggcacagcgc
    11701 gcttaatctt gtctgaccct tctccattag caaatcgagc tttcccagtt gaactgcata
    11761 cacagtttta tcccgcattc tctaatatga atatcattcg cgaacatacg gtcgagattt
    11821 ctcggtttgc gattcatagc gatttcttta gaaggaaaga tgattttcgt ttcagcgttg
    11881 acaaagctgc aagccaaggt attcgaaaaa atgaacgcag aagatttcca catcccatgc
    11941 tagggctcgc tgttggcatc attcagctat gtgctaagca tgatttcttt ttctggtttt
    12001 ctgccatgga tccggcttta aatcgattgc tgggatttta tggaatgcaa ttaaatccaa
    12061 ttggtccaat tgttgattat tatggtccac gccgacctta tcatgtctgt gttattgatg
    12121 ttttagaacg aatgtatgtc aactacccag aggtatggga acttgtaacc gacaatgggc
    12181 agatttggcc ttctgatcta caatcagttc gagagcggaa tcagcatatt ttgttagcga
    12241 gtaatgaaat ggtgacttgt taaggttcta tagcctatat ggtagcgaga aaccgtttta
    12301 attactgcta tcgaatcgtc tcgctattct tttttgtttt tggcgtgtcg tattttgcaa
    12361 cacacactta cgccaatgat tccgtcgaag cagtaattca cccgagtgtc gaattaaaga
    12421 gttattcaag aaattcttta cgcgctgcgt ttggaatgcg cctaaaaacc tggcctaacg
    12481 gcatgccagt cagggttttt gttatgcctg atgatgctgt gttgcatacc atcttcacaa
    12541 aagaaaagct caatattttt ccctatcagc ttagagctgg gtgggataaa ttaattttct
    12601 caggaacagg acaggctcct ttcttagtaa gaactgaaga agaaatgcgt gaacgtatat
    12661 ccagtactcc tggcgcgatt ggttatttaa agagagctaa catcgatgaa agcatacatg
    12721 tactcaacat tgaataaaca gtactcaata ctcaattata caatgatgtg tagactcgct
    12781 tccgtagtcc tattgcttat tattatgatg caggagggta ttgcacagga tgtaaaagga
    12841 atccgagtac atggttttct gagccaaggc tatttgaaat caacaggtaa caatgacgta
    12901 tttgggaata gcagcggaaa tggcagtttt gattttagag aaataggtgc taacgcttct
    12961 tatcggccat tgcctaactt acaattttca gggcagttgt tatcgcgaag tgcgggtgag
    13021 gatagtaaag gtggtattcg tattgactac ggttttgttg attacactgc aataagtacg
    13081 gaatcaatgg attttggtat aaggttaggt agaacgaaaa atccattagg attttataac
    13141 gatacgcgcg atgtaccgtt tacgcgaccc agcattttac tgccgcagtc gatctatttc
    13201 gatcgcactc gaaatttagg gctggcatcc gatggcgctc agatttatgg tgaatccagg
    13261 aatagttggg gagatttgaa tgcacaattt ggggttgttt ttccgcaagc gggggatcga
    13321 agtaccgagt ttgctattct gcaggcagat cgtcctggag gcatcacgcc gcacttgtct
    13381 tacattggtc gcttgatcta tgaatacgat ggtggaagag tgcgacttgc tttgagcggc
    13441 gctcaggtta atactggtta tgacgctgct caggtagacc ctttttcagg attaggatct
    13501 ttccagttta ctccgattat attttcggcg cagtacaata cggaacgttg gagtttaact
    13561 tcagaatatg caattcgcca tatcgagcgg tatgattttg ccaacagatc tgcacgcgat
    13621 ctaaacctca caggggagag ctattatttt cagggaatct accgtttctc gccgaactgg
    13681 gaaggtgtgc tgcgctatga tgcctactat gcggatcgca gtgatcgaga tggaaaggat
    13741 tttgctaaat tcatacaaat acaggctcct gattttcaag ataattttcc ttcctatagc
    13801 cgttttgcca aagatatcac ggtagggtta cgttggaatg tgacaccatc gatcatgctt
    13861 cgtgctgaat atcattatat caatggcaca ggctggattt cttcgttgga taatcgagcg
    13921 ccaaatacta ctttacaacg attcctgcct gctattaccg atcaacattg gaatttgttt
    13981 gcgttccaag cttcatatcg tttctagtag tcaatattat gaagctcagt catttggtgt
    14041 cactcactcg cctcgacagg gagatccata agacattatc aaaattcttt agcttgaaat
    14101 ggaaaatttc attgctaagt agtttgattt tgctgattgt agtcacgatg tttagcgtga
    14161 ttaattatcg aagcctcatt gataacgttg attatcagag cagtttgcaa taccaacgct
    14221 atgttaccga agttgagaat ttaatcgagc aaacgtcgaa taattctcgc caattaatta
    14281 ggttgatccc tttcttgaaa gaaatggata ctgcattagt gctgcaagat agcgctcaaa
    14341 ttacccaagc ctttgagcag cattggtcct tatttcagtt acataatggc atcgagacaa
    14401 ttcggtttta tgatcagtca gatcaactgt tggttggttg ggataacttt gaagaggatt
    14461 cgcagcgtga tgccctcata ttagagcagg tccatgatgt caattcacaa gaattaccgg
    14521 cgagtttatt gatttgtatc gaaatgtgtt tgcaatatac catcgagccc ttattgattg
    14581 atggagttcg cgccggagcg atggtcatcg gcgcttcatt agcagatgtt tttctgggat
    14641 ttaaacgaac atttggatcc gatattggat tgctcttgaa aatgaaggca gatggcgtag
    14701 atatacaaca gaatgatgtc gagttttcaa attggggcgt gcgcttagct gcactgacga
    14761 atagggaaaa gaatttagag atactccatc aaatagccca atcacatcct actattcagg
    14821 atcttagggt aggaattcat actgaatggg ataatcgtca tcaccgaatc aagctacttt
    14881 cgttgcgagg tgtcaatgta ttggatcgtg cttatttggt tgtacttaca gatattacgc
    14941 caacgattct ttccattcga gattctacct ggcggattat cattattgga ttagtgggat
    15001 tactggtatc cgaactcata ttatttgcaa tcctttcatt accccttacg agactaaagc
    15061 atattgtatt tacactaccc ctcttggcgg gtggaagctt tgggattttt cgtgctacgc
    15121 tagcttctgt tagccggaaa cggtggctta aagatgaagt agatcttcta tatgaagcag
    15181 ccgtggcgtt atcccataag cttgagaaat tagagaataa ggtcgcgtat cgtaccaaat
    15241 tgcttgtcca acaaagagat gagcttagta aagaaaaaga tttcattgct cacctgttgg
    15301 ataccgcaca agttatcgtt ctaacccaaa atgttagcgg gaagatcctc atgttgaatg
    15361 cgtatggtga actgttgatg cgctattcta aagaagaaat tcagaccaag tattttcttg
    15421 aagttttagc acccgataac gatcaacaat ataatcttgt caattacctt gaagaaattc
    15481 gccgtggaca tcgtgaccag ttgcggcatg aagcgatgac cttgtgtaat gatggctcaa
    15541 agcgccatat tgtatggctg cattcacgat taacctggca atctgaaaca gatccttcag
    15601 ttttatcggt tggtttggat attaccgaat ataagcgcgt tgaaggacat cttgcttggc
    15661 tggcggacca tgatccactg actaatttat ttaatcgacg acggtttagt gaagaattgg
    15721 agcaaatgct gagttgggca gaaagatatc agcatccagg tgctttattg ttttttgatc
    15781 tcgatcgttt taaatatatc aatgatacca gtggacacca agcgggcgat gcattattga
    15841 agatggtggc tgagatgctg tcgcgcacga ttcggactgc tgatgtaacg ggtcgcttgg
    15901 gaggggacga atttgcagtc atcttgccag aaatttctgc aggtggagcg atcgaagttg
    15961 caaagaaaat tttgttgcgc ttgagtgaaa cgcaattaaa tgtcaatgga agaacctaca
    16021 aaattgctgc aagtgtaggg atagcccttt ttcctgagca tgggaagaat gtgcacgatt
    16081 tactggctgc cgctgactta gctatgtatc aagctaaaga aattgggcga gggacgtggc
    16141 atttattttc aaatgatgac aaaacgcggg agcggctata tacactggtt cattggaaag
    16201 aaaaaatcga gtatgctaat ttatacgatc attatttact ctatttccaa ccgatcatgt
    16261 cattgcatga tcgagcgatt acgcattatg aagtgttact gcgtatgcat gacaatgatg
    16321 gcacaattca ttcacccgct gcatttatcg cagccgctga gcataccgga ctgatccatg
    16381 caattgatca catggtttta cggaaagcga ttacccaggt cgccaaaata aatagtaaat
    16441 cgactaaacc gattcgattc tccatcaatc tctcggccca tgcattcaac gacccggaat
    16501 tattaccggt gattcggcat gaattaactt catcgggtgt agatcccacg ttactatttt
    16561 ttgaaataac agaaacagcg gcgttagata acttacctgg aacgagaaat ctacttcgcg
    16621 agataaaaaa attgggttgt gggtttgtgc ttgatgattt tggcgttgga ttttcttcgt
    16681 tctattattt acgagagcta ccggtggatg ccgtgaaaat tgatggttct tttattcgga
    16741 atttagcgaa taatccagat gaccagattc tagtgagagc cctatgtagt gttgcaaagg
    16801 gttttggaaa gaaaattact gctgagtttg tagaaagtga agaaatttta atgctgctgg
    16861 gaaaaatgga tgtggactat gcgcaaggct atcatgttgg aaagccagcg gctgctgagg
    16921 atattttccg attgttcgag gaaaacgaga taagttagac ctggctgcta atcattttct
    16981 tttcgaaaga aagcgatgaa tccacccagt gataaacata atccaataat cgttagaact
    17041 aaagtattac catactgaac ataaggtgta gcgcctttaa atccctgagc tgtaccatgt
    17101 agcccttgtg ttacaaacat ttcgagttct tgcacgatct ggccgcgttc gttgatgatc
    17161 gcggttactc ctgtattggt tgcacgaagc atataacgcc ctgtctccaa tgcgcgcatt
    17221 tgagaaattt gtaagtgttg tcgtgggcca atcgagcgac caaaccaggc atc
//
LOCUS       DAOQLJ010000032        17209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1183_length_17209_cov_6.278244, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000032 DAOQLJ010000000
VERSION     DAOQLJ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 17209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17209
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1183_length_17209_cov_6.278244"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PK522_03900"
     CDS             complement(<1..548)
                     /locus_tag="PK522_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNC40907.1"
                     /translation="MAFRYHFFRYSFVSFVFANICWVNTVMGAGFAIMEQSVLELGRA
                     FSGAPTNTGDGSMVYFNPGAMTQVRSKLLTTAGYLIVPSAKFNDEGSQLNPLVGGFPL
                     RGNEGGNAANLIPIPNVYYIQPLTSKVSFGLGVNSPFGMENQYHSNWKGRYQAITSEL
                     ITININPSLAVKLTEKVSLGAG"
     gene            831..3203
                     /locus_tag="PK522_03905"
     CDS             831..3203
                     /locus_tag="PK522_03905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC40908.1"
                     /translation="MIDQNPPTPFDNRAQEIRAGQIKLLFQQTPSAFLATLLNSVILA
                     SILWGNVPKNQLISWIITVYILTLTRYLFVRYYHQIKPSSAYAIVWGRFFLLGVFLSG
                     TLWGIAGKIFFIQDSLLHQLFLIYLLGGMVAGAMSTLSSYRGAFLTFSIPVTLPVCYE
                     AFSLGSEFHIAIAATFLLFLIMLWAISQRLHLMITDSLRLRFDNADLLEHLLQAKNEQ
                     QTINEALQTQILEKERAQKTLQNINAQLEQHVMERTHDLVLTNDSLHREKELFRVTLA
                     SIGDAVITTDAKGNITYLNPIAESLTGWNNTESMGIPLHKVFQTVDQETQEYLVNTLF
                     NRLATATTINKSQECLLVRKDQQESVVDYSVAPIHNDEGNVYGIVLTFRDVTEQRNLA
                     RKLAYQASHDPLTGLLNREAFENRLNKILGAAREDNVHALLYLDLDQFKVVNDTCGHS
                     AGDELLRHVTTLLQSQLRARDTLARLGGDEFGIILEHCSKKDAIKIAQTLREAIQDYR
                     FHWQDKSFTIGASIGLYPINRSGESLSTVLSAADSACYAAKETGRNRVHVYQSDDHLL
                     QKRTGEMQWLPRIQKAIIENRLCLFFQPILATHSDPTIEKHGEILLRLRDEEGNLIMP
                     GAFLPPAERYDQMLLIDRWVVEKAMVFIKAELQHSTKVIYAINLSAQALCSEDFLDFI
                     VTQIKTHINHPASLCFEITENVALADPKHVKQFIATLKELGCLFSLDDFGSGFSSFSY
                     LKEIPIDFLKIDGRLIKDMTSDPIDLTNGAIDPKYRTRHGSQNYCGMGRR"
     gene            3166..3294
                     /locus_tag="PK522_03910"
     CDS             3166..3294
                     /locus_tag="PK522_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC40909.1"
                     /translation="MGLKTIAEWVEDEATLQLLRDMNVDYAQGFWLARPQQLDEIN"
     gene            complement(3411..4166)
                     /gene="moeB"
                     /locus_tag="PK522_03915"
     CDS             complement(3411..4166)
                     /gene="moeB"
                     /locus_tag="PK522_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNC40910.1"
                     /translation="MNDDQLLRYSRHILLPQIDINGQEKLSRSCVLIVGLGGLGCPAA
                     LYLAASGINKLIICDPDLVDLTNLQRQILHTTQSVGEAKTSSAERALYQINPTIEIIS
                     LKKRITEDVLQEYISQVDAIIDASDNFITRNLISQMCYHHKKPLISGAVTGFNGQITV
                     FDHRKQENPCYQCLFPELSTDSDVNCAVMGVFAPLAGVIGSIQAAETIKVLLDIGTTL
                     SGRLLLLDGLTMQLRTIKINKDPDCHVCGAVAV"
     gene            complement(4226..5674)
                     /locus_tag="PK522_03920"
     CDS             complement(4226..5674)
                     /locus_tag="PK522_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNC40911.1"
                     /translation="MHKLVNKVSLVVLGIIAGVMISLNFSAVADKTLKDELPALPIEE
                     LRAFTQVFGRIKSDYVEPVDDKKLITEAINGMLVGLDPHSAYLDSEEYKELQIGTQGE
                     FGGLGIQVTMEDGLVKVISPIEDTPAFRAGIKTGDLIIKLDDTAVKGMTLNEAIKRMR
                     GKPDTSITITIAREGEAEPLVFTLVRAIIKIESVKSKLIEPGYGYVRITQFQEQTGEN
                     LAKAIDKLFKESKIPMKGLVLDLRNDPGGLLNSAVGVSAAFLPANALVVYTEGRTTDA
                     KMRLLANPEYYLRGPGKDYLTGLPDAVKTVPMVTLVNGGSASASEIVAGALQDHKRSI
                     VMGTQTFGKGSVQTILPLGNNTAIKLTTARYYTPNGQSIQAKGITPDIVDESEESSRQ
                     LRESDLSRHLRSNKSESGDDNTNGGEPKKQNEAAGNKTKSKKATDSAKKKTAPAEFGS
                     SDDALLAKAITYLKNSNGNIKRKNTKTAKSAG"
     gene            complement(5714..6973)
                     /locus_tag="PK522_03925"
     CDS             complement(5714..6973)
                     /locus_tag="PK522_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNC40912.1"
                     /translation="MEQTHRVGKALRCWFFKAIPLMIVLAIAYQPFFLFTHVDAAPQP
                     SKEDLLALQNRIKALQDELAAKEVHKVEATDALQESERAISEINSVLVKLAKQQTDAN
                     NRLNELMHQTRQLEEELNQQTDQLGSLLYHQYINNRSSNFHLLLNLQDPNHIARNLYY
                     YRYLKQARVESIVHLQQYLHDIEMLKSESRVKQDEIAKVQAEQTQQKKILDQEKAKRA
                     TVLANLSEKISLQQHELSKLKADEQRLAKLVEQINQFLAKSKRDSDTASTSANAPLLN
                     NKTPDSTVSGYSFTSLRGKLRLPVRGELVNRFDSPRQEGGIKWRGLFIRAKNGNEVKA
                     IASGQVVFADWLRGFGNLMIVDHGSSYMSLYGYNEAIYRRVGDKVQGGDTIAIVGNSG
                     GSSESGLYFELRHQGKPFDPLTWVKIE"
     gene            complement(6993..7754)
                     /gene="gpmA"
                     /locus_tag="PK522_03930"
     CDS             complement(6993..7754)
                     /gene="gpmA"
                     /locus_tag="PK522_03930"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNC40913.1"
                     /translation="MDMIKLVLLRHGESTWNNENRFTGWTDVDLTARGQEEAKAAGKI
                     LRQNGFAFDVAYTSVLKRAIRTLWIVLDEMDQMWIPITHSWRLNERHYGALQGLNKAE
                     TAARYGDEQVLIWRRGYDVLPPVLTIDDERYPGFDPRYSKLTKEEIPLAESLKSTVTR
                     FLPFWNQTIIPDLQAGRSVIIAAHGNSLRALIKYLDNVSDEAIIDYNIPTGIPLVYEL
                     DKQLKPINNYYLGNQAEIEEAMLAVANQGKVHKNS"
     gene            complement(7864..10920)
                     /locus_tag="PK522_03935"
     CDS             complement(7864..10920)
                     /locus_tag="PK522_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC40914.1"
                     /translation="MNLAALCIQRPIMTILLSLTAVILGLTAYDGIPIAALPNYDTPT
                     ISVSAVLPGASPETMAAAVATPLERQFASIADLSTISSTNTQGNTQITLEFNQGRDMD
                     SAAVDVQAALLRAQRALPIEMTTMPSYRKINPADTTIAILSLTSETLLFSELNQFADK
                     LIIPALSTLPGIGEVRISGQKKFAVRIRVNPLALAARNLTIDDIAKVIADANPNSPLG
                     TLDGRRQTLTIQANRQLEHATDFANLIVSADENGRLIRLSELAIVENSFESVKTASWT
                     NGQPSITLLIYAQPGANVVAAVDAMKRGLPGINAQLPGSAQLSFYLDRSQSIREAIFD
                     VQFTFGLTVGLVILVMFLFLRKPNVTLIPALSLPISILCTIALMRVLGYTLDNISLLA
                     ITLAVGLVVDDAIVVLENIVRYIEKGLSPIRAALLGSKEITFTILSISVSLVAVFIPI
                     FFMPGVIGMLFHEFAAVVSIAVLVSAIVSLTLVPMLASRYLIQHESSTKVLVLTQWFE
                     SGFERLLTAYEYLLDWALTHRRIVLLIAISTLIVTVVLFSVLPKGFFPEEDTGRIMIV
                     IEAAEDISFPAMVELVRQTMEKIRSNPLIDNSVAFTEGAANNARMFVGLIPRSERPSM
                     TEVVQILRAETQSIPGVNVFMTPTQHLRLGSMPSKSRYQYVMRSIHSEELQTAATLMT
                     DQMRKHPVFRDVTNDLKLRGLQARLEIDRDKANQLGIDMASIRTALYSAFGERQVSTI
                     YTSSDSYRVILEVADAFRDNEKALNNLHLRSQTGGLVPLSSIAKVNREIGPLSVNHVN
                     QLESITLSFNLSAGSALGDATQLIKQFEREIQLSSSVLTNFAGDAAAFQSTQVSQLVL
                     ILAALLVIYVLLGVLYESFIHPLTILSGLPSAAVGALGILWLFDMELSVIAMIGILLL
                     IGIVKKNAIMMIDFALDAQRRLNMVPHQAIRTACLLRFRPIMMTTLAALMGALPIAIG
                     FGAGAELRQPLGLTVVGGLLFSQLITLIITPVIYLYLDPESKKTRG"
     gene            complement(10934..12172)
                     /locus_tag="PK522_03940"
     CDS             complement(10934..12172)
                     /locus_tag="PK522_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC40915.1"
                     /translation="MTEITDNKKSVGGFRRLLSKWIVVVVAIAIVLLSQQYFDHSSKS
                     NEAKPKNKVAVVLTRVEQQPVAIQIKMKATVHPKQTIVVRPQLSAVIKSVHIKEGQLV
                     KQGDKLFSFDTRDREANLAKSKAQLSKTKAELAIAQRNYQRQQALFRKNFISQAMLDV
                     ARNQVELLQNQLKFDQAAIQADQVSLSYGEIYAPISGRVGSIPFFAGSLVQPESSQLV
                     SIVQLDPIQVSFALPESELSLLQQSSTLGQIKAIVQFGSSGEETREGKVVFVDNAIDT
                     ASGTIQIKAELTNADGKLWPGMYVEVVLVKQWIPDAIVVPVQAVQSGPMGHYVFMIDT
                     TDKAIMQPISVRFIQEGIAIVDGLDFGYCIVVEGAHALLSGSTIGENSLTQPSDKMCK
                     QVGFTESSRSTSTVEMQQSK"
     gene            complement(12213..12926)
                     /locus_tag="PK522_03945"
     CDS             complement(12213..12926)
                     /locus_tag="PK522_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC40916.1"
                     /translation="MLRNIFIKNRHTQRDKQVIVQATALNKTVALGDKPLIILQDINL
                     QINAGESVAIVGASGSGKSTLLGLLAGLDVPTSGQVFLDTEAIFLLDEDQRAALRSRL
                     LGFMFQSFQLLPALTAMENVMLPIELSGLKSAKQLAQALLERVGLGARLDHYPRQLSG
                     GEQQRVAIARAFVTQPKLLFADEPTGNLDSETGKQIIELMFELNQEKGTTLILVTHDE
                     DLSNRCDRKIKMVAGRIID"
     gene            12907..13557
                     /locus_tag="PK522_03950"
     CDS             12907..13557
                     /locus_tag="PK522_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HNC40917.1"
                     /translation="MKIFLSIIFLFINLIILAPTGAESHQNRITIMILGDSLSAGYGL
                     PTESSWVTLLKNRLQNESPNYHVINISISGETTLGGRNRIEQALKLHEPNIVIIGLGA
                     NDGLRGTSIQTINDNLEMIIETCQRNHAAPILVGMQLPPNYGSTYTQKFRDLYQHIAT
                     RYQLPLIPFLLEGFADKRDHFLADGIHPNTAAQALILENVWKVLNPLIKTQMQKEG"
     gene            complement(13595..14560)
                     /locus_tag="PK522_03955"
     CDS             complement(13595..14560)
                     /locus_tag="PK522_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNC40918.1"
                     /translation="MPNAVYVENIDERSEGQRIDNFLFKHLKGVPKSHIYQILRSGQV
                     RVNSKRVTADYRLQLHDAVRIPPLKLTLKSEPTRILPRANSTRFEKLYEDEWLIAINK
                     PAGMAVHGGSGVSYGIIEQLRQDYPEWKFLELVHRLDRDTSGVLMLAKKRSALVGLHQ
                     QIREGSVQKFYWVIVKGKWTNAKQSVKLLLNKFVTAKGERRVTVTDGRNQDTKDLFSH
                     TIFTLEQSWPDYSLLKADLKTGRTHQIRVHLAHLGFPIVGDEKYGDFDFNRQLMKKHQ
                     KIKRMFLHAGTIAFIHPITNVALKVTAPLSKEWDQFIDWFKREGG"
     gene            14853..14987
                     /locus_tag="PK522_03960"
     CDS             14853..14987
                     /locus_tag="PK522_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40919.1"
                     /translation="MNKSLPDAAASRKFDFHRNNTLDLGKQKNGFMTYSADDLTITGN
                     "
     gene            15182..>17209
                     /locus_tag="PK522_03965"
     CDS             15182..>17209
                     /locus_tag="PK522_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNC40920.1"
                     /translation="MKRMLFNATQPEELRVAIVDGQKLIDLDIESVGKEQRKSNIYKA
                     VITRLEPGLEAAFVDYGGDRHGFLPYKEIDLSVIGNQSETPSHRIHEHLREGQELIVQ
                     IDKDERGNKGAALTTYISLAGRYLVLMPNNPRGGGISRRVNADERAELRDIIGRLQIP
                     DGMSIIARTAGIGRNEEELQWDLNYLTQLWHAIEEAANNQGGVFLIYQESSLVIRAIR
                     DYFYHEIGEILIDNPEIYDQARQFMTHVMPNNVDRLKLYQDDVPLFSRFQIEHQIETA
                     YSRQVSLPSGGSIVIDHTEALVSIDVNSAKAIRGTDIENTALNTNLEAADEIARQLRL
                     RDLGGLIVIDFIDMEVARNQREVEIRLQEALHVDRARIQVGKISRFGLLELSRQRLRP
                     SLGESNYITCTRCHGTGHIRGTDSSALHVLRIVQEEAMKENTSALHVQLPVDVATYLL
                     NEKRAEIHNIEARLKVNIILIPNIYLETPNYKINRLRHEDIKSVEVQASYEMVEKVAE
                     EITLPSISPENKVTRPQAAVKGITLAQSAPIREEKPISFLDKFFSWFRPTTEEQNKSE
                     TASIHAREKSSQRERGRGRRDRGRGGRNKETPANDQRKRNNLNEVHKEAHQDTESILL
                     QESRSDLTAYHDETLVSQPKDNQSSQLVHSSVELASNQPEIHELGSTEGNIK"
BASE COUNT         5108 a         3839 c         3259 g         5003 t
ORIGIN      
        1 aaccctgcgc ccagtgaaac tttttcagtt aactttaccg caagtgaagg attaatgtta
       61 atggtaatca actcagaagt aatggcttga taacgccctt tccaattgga atggtattga
      121 ttttccatac caaaaggaga attaacacct aacccaaatg atacttttga tgttaatggc
      181 tgtatgtagt aaacattagg aatcggaatt agatttgctg cattgcctcc ctcattaccg
      241 cgtaatggaa aaccacctac taagggattt aattgggaac cctcatcgtt aaattttgct
      301 gatggaacaa ttaaataacc ggcagtcgtt aacagtttgc tccgaacctg agtcatcgca
      361 ccaggattga aataaaccat acttccatcc ccagtgttgg taggtgcccc cgaaaaagca
      421 cgtcctagct ctagtacact ttgctccata atggcaaacc ctgcacccat cacggtatta
      481 acccagcaaa tgttggcaaa cacgaaactt acaaaactgt atcgaaagaa atgatatcta
      541 aaagccatct atttactcat ttgttgtcaa aatgacatct gtacgctacc gagttaatta
      601 aatagaaccc ttaacatatt attttctact tatctaataa ttagatgaca tgatactgcg
      661 gcacatttga tgttacgtca atgagttatg caataatggg tatatcttca aaaaaattca
      721 tgattgaata ttgttttatg ctttggtttt agcagttttt atcaccataa taatcattat
      781 gacgtcactg atacgctatg ctaactaaac cataagaatt gctactcact ttgatcgatc
      841 agaaccctcc cactcctttt gataatcggg cccaagaaat tcgagccgga caaatcaagc
      901 tgctttttca gcaaacaccc agtgcattct tagcaacttt acttaattca gttattctgg
      961 catcgattct ctggggtaat gttccaaaaa atcaattaat tagctggatt attacagttt
     1021 atattttgac gttaacccga tacctatttg tccggtatta ccatcaaata aaaccatcca
     1081 gtgcatatgc tatcgtttgg ggacgattct tcttactcgg tgttttttta tccggtacat
     1141 tgtggggcat tgctggaaaa atcttcttta tacaagacag cctacttcac cagctttttc
     1201 taatctatct actcggtgga atggtagcgg gtgcaatgtc taccttatct tcctatcgcg
     1261 gggcttttct cactttttcg attcccgtta cacttccggt ctgttatgaa gcgttctcac
     1321 tcggaagtga attccacatt gcaatcgctg caactttttt attgttctta atcatgcttt
     1381 gggcgatttc tcagcgattg cacttgatga ttaccgactc acttcgatta cgttttgata
     1441 atgcagattt attagagcat cttctgcaag caaaaaatga gcagcaaacg attaatgaag
     1501 cactacaaac ccaaattcta gaaaaagagc gagcacaaaa aacactgcaa aatatcaatg
     1561 cacaacttga acagcacgtg atggaacgca cgcatgatct cgtgctgaca aacgattcat
     1621 tgcaccgaga aaaagaactc ttccgagtca cactcgccag tattggcgat gccgtcatta
     1681 ccacggacgc aaagggaaat atcacttact taaatccgat tgcagaatca ctaaccggct
     1741 ggaacaatac agaatcgatg ggcattccac tgcataaagt ttttcagact gtcgatcagg
     1801 aaacccaaga atacttagtc aatacgcttt tcaatcgttt agcaactgca acaacgataa
     1861 ataaaagcca agaatgttta ttggtccgca aagatcagca agaatcagtc gtcgattatt
     1921 cagttgcacc aatccacaat gatgagggaa acgtttatgg tatcgtcctc actttccgcg
     1981 atgttaccga gcagcgtaac ttggcaagaa aactcgcgta ccaagcctcg catgatcctt
     2041 taactggatt acttaatcga gaagcctttg agaacaggct taataaaatt ctgggggccg
     2101 cccgtgaaga caatgtccat gcgctccttt atctcgattt agatcaattc aaagttgtca
     2161 atgacacctg tggacacagt gcaggtgatg aattattgcg tcacgtcaca acacttttac
     2221 aaagccaact aagagcgcgt gacacccttg cccgactcgg gggcgatgaa ttcggtatca
     2281 ttctggagca ctgctcaaaa aaagacgcga ttaaaattgc acaaacgctt cgggaggcga
     2341 ttcaagatta ccgtttccac tggcaagata aaagctttac gattggtgcc agcattggtt
     2401 tatatccgat caaccgcagt ggagaaagcc tttccactgt gttaagtgca gccgatagcg
     2461 cttgttatgc agccaaagaa actggacgta accgggttca tgtgtatcaa agtgatgatc
     2521 atttgctgca aaaacgtact ggagaaatgc aatggttacc gcgcatccaa aaggctatca
     2581 tcgagaaccg cctatgcctt ttttttcaac cgattctagc cacgcatagc gacccaacaa
     2641 ttgagaagca tggcgaaatc ttattacgat taagagatga agaaggtaat ttgattatgc
     2701 ctggtgcgtt ccttcctccg gctgagcgat atgatcaaat gctactcatt gatcgctggg
     2761 tcgttgaaaa agccatggtt ttcattaaag ccgaattgca acacagcacc aaagtcatat
     2821 atgcgatcaa tttatctgct caagccctgt gtagcgaaga ttttcttgac ttcattgtga
     2881 ctcaaatcaa aactcatatt aatcaccctg caagcctctg ttttgaaatc accgagaatg
     2941 tagcactcgc tgatcccaaa cacgtcaagc aattcattgc tactttaaaa gaattgggtt
     3001 gcttattctc tttagatgat tttggcagtg gtttttcatc atttagctac ttaaaagaaa
     3061 ttcctatcga ttttctgaaa atcgacggtc gcctcatcaa agatatgacc agtgacccta
     3121 tcgatctaac caatggtgca atcgatccaa aatatcggac acgtcatggg tctcaaaact
     3181 attgcggaat gggtcgaaga tgaagctacc ttgcaattgc tgcgtgatat gaatgtcgat
     3241 tatgcacaag gattctggct ggctcgacca cagcagttgg atgaaatcaa ttgacttcag
     3301 caaattctac tgatgcatag ccattcttac aaaattcgac tggtaataga tgctctctac
     3361 taagtctttt ttattttcta cgcgttagcg ataatcattt tatgacgtag ctaaacggct
     3421 actgctccac acacgtgaca gtctggatct ttgttgattt ttatagttct taactgcatt
     3481 gttaaaccat caagcagcaa taagcggcct gagagcgttg taccgatatc aagaagtacc
     3541 ttgatggttt cagcagcttg aatgctccct attacaccgg ctaacggtgc aaaaacaccc
     3601 attacggcac aattgacatc actgtcggtg ctcaattccg gaaataagca ttgataacaa
     3661 gggttttcct gcttgcggtg atcaaaaacc gttatttgtc cattaaatcc agtaacagca
     3721 cctgatatta gcggtttttt gtggtgataa cacatttggc tgataagatt gcgtgtgatg
     3781 aagttatcac ttgcatcgat gatggcatca acttgagaaa tatattcttg caacacatcc
     3841 tcagtgattc gttttttcag cgaaatgatt tcaatcgtgg gattaatttg ataaagcgct
     3901 cgctcagcag agcttgtttt agcttctcca actgattgcg tcgtatgtag tatctgcctt
     3961 tgtaaattgg ttagatcgac cagatcgggg tcacagataa taagcttatt gattccactt
     4021 gctgcaagat aaagtgctgc tgggcacccc aatccgccca gccctacaat gagtacgcac
     4081 gatctactta acttttcttg cccgttaata tcaatctgag ggagtaagat atgccgactg
     4141 taacgaagca gttggtcgtc attcaatcac gtacctttcc aattagattt ctcagttagc
     4201 gctaaacaaa taacttgtat cgcaattaac ccgctgattt agctgtttta gtgttttttc
     4261 gtttgatatt cccattacta ttttttaggt aagtaatcgc tttagctaac aatgcgtcat
     4321 cacttgatcc aaactcagct ggtgccgttt ttttcttagc gctatcggta gctttttttg
     4381 atttagtttt gttaccagcc gcctcatttt gttttttagg ttctccacca ttcgtattat
     4441 catcaccact ttcactcttg ttactgcgta aatggcgact tagatctgat tcacgaagtt
     4501 ggcgtgagct ttcttctgat tcatcaacaa tatccggtgt aataccctta gcctgaatcg
     4561 attgcccatt cggcgtatag tatcgtgctg tcgttaattt gattgcggta ttattcccca
     4621 gcggtagaat cgtttgcaca gaacctttac caaaagtttg tgttcccata acaatggaac
     4681 gtttgtgatc ttgcaatgcc cctgcaacaa tttcagaagc agaagcagat cctccattca
     4741 ctaaagttac cattggcaca gttttaaccg catccggtaa ccctgttaga taatctttgc
     4801 ccggtcctct taaataatac tcaggattag ctaatagccg catttttgca tccgtagtac
     4861 gcccttcggt atagactacc aaggcattag caggtaaaaa agcagctgac accccaacag
     4921 cactatttaa taacccaccg ggatcattgc gtaaatcaag aacaaggcct ttcatcggaa
     4981 ttttgctttc tttaaataat ttatcgattg ccttagccaa attttccccg gtttgctctt
     5041 gaaactgggt aattcttaca tacccataac cgggttcgat taattttgat ttaacgcttt
     5101 cgattttgat gatagctcga actaatgtga aaaccaaagg ctcagcttca ccttctcgag
     5161 caatcgtgat agtaatcgaa gtatctggct taccgcgcat ccgcttaatc gcctcattca
     5221 atgtcattcc ttttacggca gtatcatcca gtttaatgat taaatcacct gttttaatcc
     5281 ctgcgcgaaa agctggtgta tcttcaatcg gtgagattac tttaaccaga ccgtcttcca
     5341 ttgtcacctg aataccgagt cccccaaatt caccctgtgt accaatttgt aattctttat
     5401 attcttcact atccagatag gcagagtggg gatcaagccc aacaagcatc ccattaatcg
     5461 cttcagtgat cagtttctta tcatcaactg gctcaacata atcgctcttg atgcgaccaa
     5521 aaacttgagt aaacgctcgt aattcctcga ttggaagagc tggaagctcg tcttttaggg
     5581 ttttatcagc gactgcagaa aaattaaggc tgatcattac ccccgcgata atacctaata
     5641 caaccaagct aactttatta acaagcttat gcattacttc ctcacctatc gactataatc
     5701 attaatctca aattcattct attttgaccc aggtcagggg atcaaatggt ttaccctgat
     5761 gtcgtaattc aaagtataac cctgattcac ttgatccgcc gctattccct acaattgcaa
     5821 tggtatcccc accttgtact ttatcaccaa cacgtctata aattgcttca ttataaccat
     5881 ataaactcat gtagctacta ccatgatcaa caatcattaa attaccaaat cctcttaacc
     5941 aatcagcaaa aaccacttga ccgctcgcaa tcgcctttac ttcgttgcca tttttagcgc
     6001 gaataaataa gcctcgccat ttaattcctc cttcttgacg tggactatcg aatcgattga
     6061 caagttcccc tcgaactggt aaacgcaact tacctcttaa ggaggtaaaa ctatagccac
     6121 tcacagtaga atctggagtt ttattattca ataacggagc attcgctgat gtactagctg
     6181 tatcgctatc acgcttggat ttagcaagga attgattaat ttgttcgaca agtttagcaa
     6241 ggcgttgctc atcagctttc agcttactca attcatgctg ctgcaaggaa attttttctg
     6301 aaagatttgc taaaactgtt gcacgtttgg ctttctcttg atctaaaatt ttcttttgct
     6361 gagtttgctc agcctgtact ttagcaattt catcttgttt tactcgactt tcgcttttta
     6421 acatctcgat atcatgcaaa tattgttgta aatgcacgat actttcaaca cgcgcttgct
     6481 ttaaatatcg atagtaataa agattccgcg caatatgatt cggatcttgt agatttaaca
     6541 gtaaatggaa attgctggat cgattattaa tatattgatg atacagtaaa gaaccgagtt
     6601 gatcggtttg ttgattaagt tcttcttcaa gttgacgggt ttgatgcatg agctcattta
     6661 agcgattatt tgcatcagtt tgttgctttg caagcttcac gagtacgcta ttaatttcac
     6721 taattgcacg ttctgattct tgtagcgcat cggtagcctc aactttatgc acttctttcg
     6781 ctgccagttc atcttgaagt gcttttattc gattttgtaa cgctaataaa tcctctttac
     6841 tcggttgcgg tgctgcatca acatgcgtaa aaagaaaaaa gggttggtag gcaatcgcca
     6901 atactatcat tagcggaatt gccttgaaga accagcatct caaggccttt ccaacacgat
     6961 gagtttgttc catttcaggg attggaactt aatcaactat tcttgtgaac tttaccttga
     7021 ttcgcgacag caagcattgc ttcttcaatc tcagcctgat tacccaaata gtaattattg
     7081 attggcttta attgtttatc tagttcgtaa actaatggaa tgcctgtcgg aatgttatag
     7141 tcgataatcg cttcatcaga aacattatcc aaatatttta tgagggctct gagtgagttg
     7201 ccatgcgctg cgataatcac actccgtccc gcttgcaaat caggaatgat ggtttgattc
     7261 cagaatggta agaatcgagt aaccgtactc tttaaactct ctgctaacgg gatttcttct
     7321 ttggtgagct tactgtaacg aggatcaaaa ccagggtatc gctcatcatc gatcgttaaa
     7381 acagggggca atacatcata gcctcgccgc caaataagca cttgttcatc accataacgt
     7441 gcagccgttt cagctttatt taacccttga agtgcaccat agtgccgttc attcaatctc
     7501 caagagtgcg taatgggaat ccacatttgg tccatctcat ccaacacaat ccataatgtt
     7561 cggattgcac gtttaagcac agaagtatag gcaacatcaa aagcaaatcc attttgacgc
     7621 aatatctttc ctgccgcttt cgcttcttct tgacctcttg ccgttaaatc gacatctgtc
     7681 caacctgtaa aacgattttc attattccaa gtactttctc catgacgcag gagaacaagt
     7741 ttaatcatgt caatatctta agagtgggta atttctaagg agcaattcaa taatcggtaa
     7801 ttctattaca tttgacctaa aaacgcagca aagataggca tagaattcat cgcttttctc
     7861 cgattatcct cgagtttttt tggattcagg atcgagatat aagtagatga cgggggtaat
     7921 aatcaaagta atcaattgag aaaaaagtaa tccgccaaca accgttagac ctagcggttg
     7981 acgtaattca gcacctgcac caaaaccgat cgcaatcggt aacgcaccca ttaatgctgc
     8041 tagtgtcgtc atcataatcg gacgaaagcg aagcagacaa gctgttcgaa tcgcttggtg
     8101 aggaaccatg ttcaatctgc gctgcgcatc taaagcaaaa tcgatcatca taattgcgtt
     8161 ctttttcaca atcccaatca acaaaagaat tccgatcata gcaatcaccg ataactccat
     8221 atcgaatagc cacaaaatac ctaacgcccc aactgcagca gaaggtaatc ccgaaaggat
     8281 agtgagcgga tggataaaac tttcatacag cacacccaat aaaacataaa tcaccaataa
     8341 agcagccaag ataagtacca attggctcac ttgggtcgat tggaaagccg ctgcatcacc
     8401 cgcaaaattc gttaataccg atgaagataa ttgaatctca cgctcaaatt gcttaattaa
     8461 ttgcgttgca tccccaagtg ccgatcccgc tgaaagatta aacgataggg taattgattc
     8521 gagctgattt acatgattaa cgctcaaagg acctatttct cgatttactt tagcaatact
     8581 cgataaagga accaagcccc cagtctgtga gcgtaagtgt aaattattta atgctttctc
     8641 attgtcccgg aaagcatcag ccacttccaa aataacccga tagctatcac tggatgtata
     8701 aatggttgaa acttgccttt ccccaaaagc gctgtataaa gcagtacgaa ttgaagccat
     8761 atcaattccc aattgattcg ctttatctcg atcaatctcc agtcgtgcct gtaatcctct
     8821 gagtttaaga tcattcgtta catcgcggaa taccggatgt ttacgcattt gatccgtcat
     8881 cagggttgct gcggtctgca attcttcact atgaatgctg cgcatcacat attggtatcg
     8941 acttttactc ggcatactcc ctaaccttaa atgctgtgtc ggggtcataa aaacatttac
     9001 gcctggaatc gattgagttt cagcacgcag aatctgaaca acctctgtca tcgaaggacg
     9061 ttcactacga gggattaatc ctacaaacat acgcgcattg ttcgcagcac cttccgtaaa
     9121 tgcaacactg ttatcgatca acggattcga tcgaatcttt tccatggttt gtcgaaccaa
     9181 ttccaccatc gccggaaaag aaatatcctc agcagcctcg attacgatca tgattcgacc
     9241 ggtgtcttct tcaggaaaga accctttagg tagcacacta aaaagaacga cagtaactat
     9301 taaggtactg atagcaatta atagtacaat ccgacgatgg gttaaggccc agtctaatag
     9361 atattcatac gctgtcagaa gtcgttcaaa ccctgattca aaccattgag tcaataccaa
     9421 taccttcgtt gaagattcgt gttgtatcaa gtaccgactt gctaacattg gcaccaatgt
     9481 caatgaaaca attgctgaca ccaatacggc tatacttact actgcagcaa actcatggaa
     9541 taacatgcca attacgcccg gcataaaaaa tatgggaata aaaacggcaa ccagcgaaac
     9601 cgaaatcgac aaaatggtaa atgtgatttc ttttgagccc agtaatgcag cccttattgg
     9661 ggacaagcct ttttcgatat accgaacaat attttccaga actacgattg catcatcaac
     9721 gactaaaccc actgcgagtg taatcgcgag taatgaaata ttatcgagcg tataacccag
     9781 aacacgcatc aaggcaatcg tgcataatat tgaaatgggt agtgataaag cgggaattaa
     9841 agtgacatta ggtttacgta aaaacagaaa catcactaga ataactaaac ccacggtaag
     9901 cccgaaggta aattgcacat cgaaaatcgc ttcacgaatc gactgtgagc gatccaaata
     9961 aaatgataac tgtgccgacc caggtagttg agcgttaatt cctggcaacc ctcttttcat
    10021 ggcatcgact gcagcaacca cattggcacc aggctgtgca taaattaaca acgtaatgga
    10081 gggctgccca ttggtccagc ttgcggtctt tacagactca aaactattct caactattgc
    10141 aagttcagat aatcgaatca atcgtccatt ttcatccgcc gacacaataa gattagcgaa
    10201 atcagtcgca tgctctaatt gacgattagc ttgtatggta agcgtctgac gtctaccgtc
    10261 gagggtgccc aatggagaat tgggatttgc atctgcgata actttggcaa tatcatcaat
    10321 cgttaagtta cgcgcagcaa gtgcgagagg attgacacgg atacgaactg caaacttttt
    10381 ctggccacta atccgaactt caccaatccc tggtaatgtt gataaagcag gaataattaa
    10441 cttatcggcg aactgattta attcagaaaa gagtaaagtt tctgatgtaa gcgaaagaat
    10501 ggcaatcgta gtatctgcag gatttatctt tcgataggat ggcattgttg tcatttcaat
    10561 cggcaatgcc cgttgcgctc tcagcaatgc tgcttgaaca tcgacagcag cactatccat
    10621 atcacgacct tgattaaact ccagagtaat ttgcgtatta ccttgggtat tcgttgaact
    10681 gattgtagat aaatccgcta ttgaagcaaa ctgacgctcc agtggtgttg ccactgctgc
    10741 agccatggtt tcaggacttg caccgggcag tacagcactt accgatatgg tgggtgtatc
    10801 ataattaggt aaagctgcaa tcggaattcc atcgtacgca gtcaacccaa gaatgaccgc
    10861 agttaatgac aaaagtatcg tcatgatggg acgttgaata cacaatgccg cgagattcat
    10921 cgattaccta aggttattta gattgctgca tttccactgt actcgtggaa cgcgaagact
    10981 cagtaaatcc tacttgctta cacattttat cgctcggctg tgttaatgaa ttttcaccaa
    11041 tcgtactgcc tgaaagaagt gcatgagcac cttccacaac aatacaataa ccaaaatcga
    11101 gaccatccac tatcgcgatc ccttcctgaa taaatcttac ggatatcggt tgcataatcg
    11161 ctttatcagt tgtgtcgatc ataaacacat aatgacccat tggacctgat tgcactgcct
    11221 gtactgggac aacaatagca tcaggaatcc actgcttgac aagaactact tcaacataca
    11281 tgccaggcca aagttttcca tctgcgtttg taagctcggc tttaatttga atcgtgccac
    11341 tcgccgtatc gatcgcgttg tcaacaaaga caacttttcc ctcccgggtt tcctcgccgg
    11401 aagatccaaa ttgtacgatg gctttaattt gacccaacgt cgatgattgt tgcaataaac
    11461 ttaattcgct ttctggaagc gcaaaactca cctggatcgg atcaagttgc acaatactca
    11521 ctagctgaga tgactcaggc tgaactaagc tgccagcgaa aaatggtatg ctcccaacgc
    11581 gcccactaat cggcgcataa atttccccat aactcaaact aacctgatct gcctgaatag
    11641 cggcttgatc aaatttaagc tgattctgga gtaactcgac ttgatttctt gccacatcca
    11701 acatcgcctg tgaaataaaa ttcttcctaa ataatgcttg ttgacgctga tagttacgct
    11761 gcgctatcgc taattcagcc ttggtttttg atagctgagc ctttgacttc gctagattgg
    11821 cttcacggtc tcgcgtatcg aaagaaaaca acttatcgcc ctgcttcacg agctgtcctt
    11881 ctttgatatg aacagattta atgacagcac tcaattgagg acgcaccaca atggtttgct
    11941 taggatgcac cgttgctttc atcttaatct ggattgctac gggctgttgc tcaacgcgcg
    12001 ttaataccac tgccacttta tttttcggtt tagcttcatt tgatttagaa ctgtggtcga
    12061 aatattgctg actcaataaa acgatcgcaa tggccacaac cacaacaatc catttggaaa
    12121 gaagacgcct aaaaccccct acactttttt tattgtcggt aatctcagtc acgtcaaata
    12181 cctatttcgc cctaaacgat cgaatcagtc acttaatcaa tgattcgacc agcaaccatt
    12241 ttgatttttc ggtcacatcg attcgataag tcctcatcat gggtaaccag tattaatgtt
    12301 gtcccttttt cttgattcaa ttcgaacatc aactcaataa tctgtttacc ggtttctgaa
    12361 tctagattac cggttggttc atcagcaaat aacaatttgg gctgcgtcac gaaagctcga
    12421 gcaatcgcaa cgcgttgttg ttcgccaccc gaaagctgcc taggataatg atcaagacgc
    12481 gcgcctaaac ctaccctttc aagtagtgct tgagccaatt gttttgcaga ttttagcccc
    12541 gaaagttcta tcggtagcat tacattttcc attgctgtta acgctggcaa cagttgaaaa
    12601 gattgaaaca taaagccaag tagtcgcgaa cgaagtgctg cacgctgatc ttcatcaagt
    12661 agaaaaatag cttcagtatc tagaaaaact tgaccactgg ttggcacgtc gagacctgct
    12721 aataacccta gcagggttga tttaccagaa cctgatgcac ccacaatagc aaccgattcc
    12781 cccgcattga tttgaagatt gatatcctgc aggatgatca atggcttatc acccagggca
    12841 accgttttat ttagcgcagt tgcttgtaca ataacttgtt tatctctttg ggtatggcgg
    12901 ttttttatga aaatatttct tagcattatt tttctattta ttaatcttat tattctggct
    12961 cctaccggag ctgaatccca tcaaaatcga ataaccatca tgattctggg tgatagcttg
    13021 tcagcaggtt acggactacc aaccgaatcc agttgggtca cgttacttaa aaatagacta
    13081 caaaatgaat cacccaacta tcatgttatt aatattagta tcagcgggga aacaacactt
    13141 ggaggtcgca accgaattga acaagcgctc aaattacatg aacccaatat cgttatcatt
    13201 ggtttaggcg ctaacgatgg gttacgaggc acttccatcc aaacgattaa tgataactta
    13261 gaaatgatca tcgaaacgtg ccaacgcaat catgctgcac caatattggt tggaatgcag
    13321 cttccgccga attatggcag cacttataca caaaagttcc gtgatctcta ccaacacatt
    13381 gctacacgct atcaactacc gcttatccca tttttactcg aaggatttgc agataaacgt
    13441 gaccactttc ttgcggatgg aattcatccc aatacagcag ctcaagcgct cattcttgaa
    13501 aatgtttgga aagtgctcaa ccctttaatc aaaactcaaa tgcaaaaaga aggctaaacc
    13561 cttaatctat tctagagaaa atatttaaat tcctttaacc gccctctcgt ttaaaccaat
    13621 caataaattg atcccattct ttagacaaag gagccgtcac ttttaaagca acgtttgtta
    13681 taggatgtat aaacgctatc gtccccgcat gtaaaaacat ccttttaatt ttttgatgct
    13741 tcttcatcaa ctgtcgattg aagtcaaaat caccgtattt ctcatcaccg acaattggaa
    13801 agcctaagtg tgccaaatga acacgaattt ggtgtgttcg tcctgttttt aaatccgctt
    13861 ttagcaaact ataatcaggc cagctttgtt caagtgtaaa tatcgtgtgt gaaaatagat
    13921 ctttcgtatc ttgatttctt ccatctgtaa cagtgacacg tcgttcaccc ttcgctgtca
    13981 caaatttgtt taataacaat ttcacgctct gtttggcatt agtccacttt cctttgacta
    14041 tcacccaata aaacttctga acggaaccct cacgaatctg ctggtgtagg cccactaaag
    14101 cgctacgctt cttagccaac attaaaacac ctgaggtatc ccgatcaagg cggtgtacta
    14161 attcaagaaa cttccactcg gggtaatcct gccgcagttg ctcgattatt ccataactga
    14221 caccactacc accgtgaacc gccattcctg ccggtttatt gatagcaatc agccattcat
    14281 cttcatataa tttctcgaaa cgggtagaat tagcgcgtgg tagaattctt gttggttctg
    14341 attttaaggt taacttaagc ggtggaatac gaacggcgtc atgcaattgt aaccggtaat
    14401 ctgccgtaac tcgtttgcta ttgacgcgaa cttggccact gcgcaagatt tgataaatgt
    14461 ggcttttagg cacacctttc aagtgcttaa aaagaaaatt atcgatacgt tgaccttccg
    14521 atctttcatc aatattttcc acgtaaactg catttggcat attttctttg cctaacactt
    14581 taattttacc tatactttta ggttgagtaa ttaattgaat tgatatacct atatcattta
    14641 atgtgtcaaa tcgttgacat catggctcat gctaaacaag atttagccat aggtagattt
    14701 ggcaatgctg aaaagtcggc accaaatctg gttactgtcg ctcacctgat caagtagcga
    14761 taataaatga attacgcgct caaagcactt caaggattta tttacaaatt ttgtagtacc
    14821 ttacagtagg aataactttg ccgaaatttg ccatgaataa atccttacct gatgctgcag
    14881 caagtagaaa atttgacttt caccgcaata acacactaga cctgggtaaa caaaagaacg
    14941 ggttcatgac gtatagtgcg gatgatttaa cgattacagg aaactaagtt agtgttaacg
    15001 gcatttattg aaatgttgat ttaacttggt aaattactta ctcgaatgat agggaaacga
    15061 caaacccaat tattgtattt atgatttatg tttactcgat cacctgaaaa tatgtcattc
    15121 cctttggaac tacttagctg atattagcaa cctgcccgtg aattgagcgt gggaggaaat
    15181 aatgaaacga atgttattta atgcaacaca acctgaagag ttgcgtgttg caattgtaga
    15241 tggacaaaaa ctaattgatc tcgatattga atcagtcgga aaggagcaac gaaaaagcaa
    15301 tatttacaaa gcggtaatca ctcgactaga acccggttta gaagcagctt ttgtcgatta
    15361 tggtggcgat cgccatggat ttcttcccta taaggaaatt gatttatcgg tcatcggtaa
    15421 tcaatctgaa accccaagtc atcgcattca tgaacacctt cgtgaaggac aggaattaat
    15481 cgtccagatc gataaagacg aacggggtaa taaaggagca gcgctcacta cctacatctc
    15541 attagcaggc cgctatcttg tgctcatgcc caataatcca cggggtggtg gtatttcacg
    15601 gcgagttaat gccgacgagc gtgctgaact gagagacatt attggtcggc tccaaatacc
    15661 agacggcatg agtatcattg ctcggacagc cggaattgga cgtaatgaag aagagctgca
    15721 atgggactta aattatttga cacagctctg gcatgcgatc gaagaagcgg cgaataatca
    15781 gggtggtgtg ttcttaattt accaagaaag cagcttagtc atccgcgcga ttcgtgacta
    15841 tttttatcat gaaatcggtg aaatattgat cgataaccct gaaatctatg atcaggcgcg
    15901 tcaatttatg actcatgtca tgcctaataa tgtagatcga ctcaagctct accaggatga
    15961 tgttccctta ttctctcgtt tccaaattga acaccaaatc gaaaccgcct attcaaggca
    16021 agtttcgcta ccgtctggtg gatcgatcgt tatcgatcat actgaagcat tggtgtcaat
    16081 tgatgttaat tctgcaaaag ccattcgagg cacggatatc gaaaataccg ccttgaatac
    16141 taacttagaa gctgcagatg agattgctcg gcaattaaga ctccgcgatt taggcgggct
    16201 catcgtaatc gattttatcg atatggaggt tgcacgaaat caacgagagg tggaaatacg
    16261 cctgcaagaa gctttgcatg ttgatcgtgc acgaattcaa gtcggtaaaa tttcacgctt
    16321 tggtctatta gaattatctc gtcaacgact acgcccttca ttaggcgaaa gtaattatat
    16381 cacttgcacg cgttgccatg gcacaggaca tatacgtgga acagattcat ctgctttaca
    16441 tgttcttcgg attgttcaag aagaagccat gaaagaaaac acgagtgctt tgcatgttca
    16501 attacccgtt gatgtcgcaa cctatctgct aaacgagaaa cgtgcagaaa ttcataacat
    16561 tgaagcacgc ctcaaagtaa atatcatcct cattcccaat atttatttag agacaccgaa
    16621 ttacaaaatt aaccgactcc gtcatgagga catcaagtca gttgaagttc aagctagtta
    16681 tgaaatggtt gaaaaagtag cagaggaaat aacgctccct tcgatttcac cagaaaataa
    16741 agttacacgt cctcaagcag ctgttaaagg catcactttg gctcaatctg caccaattcg
    16801 tgaagaaaaa ccaatttctt ttttagataa attcttcagt tggttcagac cgacaaccga
    16861 ggagcaaaat aaatcagaaa ctgcatctat tcacgctcgg gaaaaatctt ctcagcgtga
    16921 gcgaggaaga ggacgtagag atcgtgggcg tggtggtcga aacaaagaga cacctgccaa
    16981 tgatcaacgc aaacgtaata atctaaatga ggtccataaa gaagcacatc aagatacaga
    17041 gagtattctg ttacaagaat cgcgatccga tcttactgcc tatcatgatg agacgttagt
    17101 gagccaaccc aaagataatc agagttctca actcgtacac agctcagtcg aactagcatc
    17161 gaatcaacct gaaatacatg aattggggtc aaccgaaggc aacattaaa
//
LOCUS       DAOQLJ010000033        17162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1186_length_17162_cov_5.664348, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000033 DAOQLJ010000000
VERSION     DAOQLJ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 17162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17162
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1186_length_17162_cov_5.664348"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1229
                     /locus_tag="PK522_03970"
     CDS             228..1229
                     /locus_tag="PK522_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HNC40921.1"
                     /translation="MMMRKLTVSLLSIGVIMASMHVTAANEPIQPIKPVKPENAELVE
                     LGKMLFFDPRLSKSGFISCNSCHNLSMGGTDNIQTSIGHKWQQGPINAPTVLNASMNL
                     AQFWDGRAKDLKEQAGGPIANPGEMASTHDIAVKVINSMPQYQAQFKKAFGSDGVDID
                     RVTTAIAAFEETLVTPGSRFDKWLEGDKNALSKEEQDGYNVFKTSGCAKCHNGPAVGG
                     NSFEKMGVHKPYETKSTAEGRKGVTGKEEDNLHFKVPTLRNVELTYPYFHDGGAATLE
                     EAVDTMAKIQLNKELSKAEIANVVAFLKTLTGKQPDFKLPILPPSNNNTPRPEPFDK"
     gene            complement(1333..3381)
                     /locus_tag="PK522_03975"
     CDS             complement(1333..3381)
                     /locus_tag="PK522_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNC40922.1"
                     /translation="MIEFFSALVRWLQLTANLVLFGSCVFLTLIWFGKKLPELGWVRK
                     LEQLFPWLVGIVLLGLIGILASTAGQATGDAINTWDLNAWWEIVLKTRVGHLWVARFG
                     LALLLLFIVLRLKRMHRVRWHYGLLAVTAALPLTVGSLVSHAGAEEISFASVTPYTLH
                     ILLAGVWFGTLPAIIYIIRDALNNSQAGTLLLHADFLKQFSVLALPVMILLFLTGWMA
                     ADRMIGDYYHTLVSSRYGWLLNLKLSILGIILWIAYRIRSVWLPLFIQENVRAGVAIG
                     QLKKWVGIEFLLALILVLVATLLANTLPGKHAIIDHWPYPFRLSIDATWDEPGVQNFF
                     WAGTLLFILAIGIAAMGTLNRWNQSKRVILPVIMGLVSLGIALPPLIIEAYPETYQKT
                     PIPFDAISISKGATLFAEHCVSCHGPQGKGTGTAPELFVADPETGEFPPDPTDLLTKT
                     HTARHTVGNFYHWLSYGVEGTSMPGFSAVLTDENRWDVVNYLHALSRGYMGRLLGSLV
                     TPNQPFLAPPVFSYFAHDGSGGSLKDFREKSNVLLVLFSWPQSQQRLFQLSDIYEMLK
                     NDFHTEILLIPNRALTEEEMAAVTRIVPFPVITEGWSEIRDSYLLFRRTMAVPDLLRK
                     GMTPEHIEYMIDRFGYLRARWVSQFDGFGWLITGVLTKQLTILNQEEKIKPPPDDHVH
                     "
     gene            3653..4927
                     /locus_tag="PK522_03980"
     CDS             3653..4927
                     /locus_tag="PK522_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNC40923.1"
                     /translation="MSDPIITCPNCHAAIHLTESLAAPLLAATRQQFQQQLAQKDEAI
                     SQREQAIRQQEQLLLEAKRNVDQQITEQVAAQLKAERDHLIADEAKKAKLAVAVELEN
                     NLRELANLKELLQTREQKLSEAQAAQADLLKKQRELDDTKRELELTIEKRVQQNLTTI
                     RNQARQEAEESLKLRVMEKDQTIAAMHQKIEELKQKAEQGSQQLQGEVQELELEALLR
                     SKFPQDSIEPVPKGEFGGDILHAVISPSGLPSGLILWESKRTKNWSESWLSKLRDDQR
                     NAKAELSVLISQALPKGIETFDIVDGVWVVHPRATLPVATILRHMLLQMSRTKQVSEG
                     QQTKTELMYQYLTGPRFRQRVEAIVEAFTTMQTDLDKERKAILKQWAKREEQITHVME
                     ATVGLYGDLQGIAGKSLQEIEGLELEYLEIKD"
     gene            complement(5052..5855)
                     /locus_tag="PK522_03985"
     CDS             complement(5052..5855)
                     /locus_tag="PK522_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNC40924.1"
                     /translation="MVYVYPVISRRSGGVSIGINLNPNNACNWRCIYCQVPNLTRGSA
                     PKIDLPLLEQELRIFLRELLEGDFMAQQVPPECQRVNDIALSGNGEPTSAKEFCEVIE
                     VIARVKHDFALAESIKLVLITNGSLINRVAVQRGLARMATLNGEVWFKFDSAIPDRRQ
                     LINGASISNGTIKRHLKIATTLCPTWIQTCMLQIDNQPPCESEVDAYLEFIAQARDAQ
                     IPLQGVLLYGLARPPMQVEADRLSKVSDQWLAQLADRIKALGIEVKAYP"
     gene            5927..6382
                     /gene="queD"
                     /locus_tag="PK522_03990"
     CDS             5927..6382
                     /gene="queD"
                     /locus_tag="PK522_03990"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNC40925.1"
                     /translation="MLITRRLEFDAGHRISTHTSQCRHLHGHRYVIEVTLSGNIIAEE
                     GVPEQGMVMDFSEIKRIVREQIVDPWDHAFLVYKNDTMVVDFLRSLENHKTVILDSQP
                     TAENLANVAFLILDEAFQKNYGDRLRLERVRLYETPNCWADATRAHHGI"
     gene            6409..7194
                     /locus_tag="PK522_03995"
     CDS             6409..7194
                     /locus_tag="PK522_03995"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNC40926.1"
                     /translation="MFIDSHCHLDFPDLAANLPELLDNMVQNQVSHALCVSVNMEDFP
                     RVRALAENYDHLYASVGVHPDYENITEPTVAQLVELAQHPKVIAIGETGLDYFRLKGD
                     LEWQRERFRRHIRAAKATQKPLIIHTREAAEDTLRIMQEEDAATIGGVMHCFTENWDV
                     AQRAIEMNFYISFSGIVTFKNALMLKEVARKVPLNRMLIETDSPYLAPVPHRGKTNQP
                     AFVKHVAEEIAKLREKSLDEIATATTDNFNTLFRLPHTSLTTH"
     gene            complement(7197..7871)
                     /gene="radC"
                     /locus_tag="PK522_04000"
     CDS             complement(7197..7871)
                     /gene="radC"
                     /locus_tag="PK522_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNC40927.1"
                     /translation="MAISDWPELERPREKLLHYGPASLSDSELLAIFLRTGITGKSAV
                     DLARELLSHFGSLTELFSADQRSFCQIPGLGVAKYVQLKATLEMAQRALETGLKRGDI
                     MTSPQQVRQFLRLSLVHRQHEVFMGLFLDARNRIIQSEELFSGTLTETSVYAREVIKR
                     ALFHNAAAIIFAHNHPSGHAEPSQADEVLTQSLKQALAYVEVKVLDHFIVGSEGITSF
                     AERGLL"
     gene            8020..9225
                     /gene="coaBC"
                     /locus_tag="PK522_04005"
     CDS             8020..9225
                     /gene="coaBC"
                     /locus_tag="PK522_04005"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNC40928.1"
                     /translation="MDSISALTGKKLILGITGGIAAYKTAELARLLVKEGAIIQTVMT
                     QAACRFIGPETLHALTGNPVYTELWGDSLQNSMAHIGLSRSSDAILIAPTSANFIAKL
                     AHGVADDLLSTLCLARDCPLIIAPAMNKQMFENPATQRNLNTLRADQITIWGPANGDQ
                     ACGENGLGRMLEPEQLQQALQVFFEPKWLDGKKVLITAGPTYEAIDAVRGIINLSSGK
                     MGYAIANAASNAGATVTLIAGPVHLPKPSVQKFISVTSATQMLAAVKKEVSNCDIFIS
                     VAAVADSRPQPYQPQKIKKANQPLTLELVPNPDILHYVAHLKKPPFCVGFAAETEKLD
                     QYAQKKRQQKRLPLIVANLAQQAMGADESTLILFDDNGKHVLPQASKTELARQLIKHI
                     AQLFEKQNA"
     gene            9419..11410
                     /locus_tag="PK522_04010"
     CDS             9419..11410
                     /locus_tag="PK522_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNC40929.1"
                     /translation="MVVTSYRKPICASKTHWQLSLFLLLAPFINSAFAAQNIQTIRNL
                     ADASLEELMNVKISTVFRKPQTLQETAAAVFVISQEDIRRSSANSIPELLRMAPGLSV
                     ARIDSNKWSVTSRGYSGRFADDLLVMIDGRTVYSPLFAGTFWETLDLPLEDIDRIEVI
                     RGPGGTIWGANAVNGAINIITKHTKATQGNLITTGGGSEERGFATFRHGGRVGENLFY
                     RFYAKGFTRDNSFNASGTNDSWRMGTLGFRADWDINTNNAVTFQAQYYAGKAGQNTML
                     LNPTEPESAITRPEDANLAGGHALMRWQRTTGERSNTALQVYYDRTSRNELSFRETRQ
                     TVDIDFQHRFPLQIPYIPFQPDLIWGGGYRWTTDHLGINLPISFDPAKRNLQTGNVFM
                     QTDIPLFEDRLKLTTGIKFLDNTYAHSNFLPNARLLWTPDDKQSVWLSATRSIRLPSR
                     FERDGNQLIRSGAEFIRLISNPHLLAETLWGFETGYRRQVTSNLSVDIAGFFNDYNNS
                     TSERETTAETIQLTNQRKTQIFGFEIYGEWRAFDKLRFMPSYSHLQVRNHVPADHESE
                     SGEDPKHQFTLRTQLDLTKNIELDAFFRHIDKLPGLEIKSYQALDLRLSWRPRSDTEL
                     TLVGQNLLQSHHQEFSPEVIQTMPVQIQRGVFGKVTWRF"
     gene            11431..12012
                     /locus_tag="PK522_04015"
     CDS             11431..12012
                     /locus_tag="PK522_04015"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNC40930.1"
                     /translation="MLKLLMQYFYNLGSLFSHKKPSAILYLLLIGISAILPKPLQAEE
                     VSEYGLKALFLYNFAIFTSWPGSNIDKFNLCIYGDNPFGQDINFLMKSKKINERTIDI
                     HRIDNVSQLDQCQLVFISRPMISNLADVITTVKDKPILTIADSPGACHQGVILNMNVN
                     NEKVTFEANLMMAKKAGLNLSSQLLRLATEIYQ"
     gene            12239..13300
                     /locus_tag="PK522_04020"
     CDS             12239..13300
                     /locus_tag="PK522_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HNC40931.1"
                     /translation="MRVILCLVLIVSSYAYAQSGHDHQHKSQKEDTQQGGMQGHGDGH
                     HGMGGMMMHGGHHKLDGATLKHFFEPLPASIIDEKKNATLIALGKKLYLDPRLSINDT
                     ISCNSCHQLNNFGVDSQPTSPGHDGTRGGRNSPTTFNAALHIAQFWDGRAKDVEEQAL
                     GPILNPIEMGMPNEEAVVNKLKKIDEYKTLFANAFKDEKDPIQYKNIGKAIGAFERTL
                     LTPSRFDDFLKGDENALNDAEKRGLTKFVHMGCANCHNGVAIGGNSYKKIGLVEPYET
                     KDLGRYEVTGLETDKKVFKVPSLRNIAQTGPYFHDGSIATLEQAIEEMAEHQLGREVG
                     PGFIEDVKAFLGSLTSKSK"
     gene            13396..13839
                     /gene="dut"
                     /locus_tag="PK522_04025"
     CDS             13396..13839
                     /gene="dut"
                     /locus_tag="PK522_04025"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNC40932.1"
                     /translation="MKIDIKILDTRLQTQLPSYATPGSAGLDLRACTEQPLTVHPGET
                     QLIPTGIAIHIEDPGFAAVVLPRSGLGHKHGIVLGNLVGLIDSDYQGQIFVSCWNRGQ
                     TEFLLNPLERIAQLVVIPVVQAQFNIVENFAMSHRGEGGFGSTGR"
     gene            13836..14285
                     /locus_tag="PK522_04030"
     CDS             13836..14285
                     /locus_tag="PK522_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNC40933.1"
                     /translation="MIRSNLFILSFCLGLTLSPLLLAENLVVSLTIGGHPLNAEIAHT
                     YDTRSKGLMDRATLPENQGMLFIFPKIDFYSMWMLNTSLPLSVAFIDEKGIILNIEAM
                     EPFSTVAHASIKPAKYALEMHQAWFTSRKITAGHKIIGLENVPDAIE"
     gene            complement(14313..15659)
                     /gene="ffh"
                     /locus_tag="PK522_04035"
     CDS             complement(14313..15659)
                     /gene="ffh"
                     /locus_tag="PK522_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNC40934.1"
                     /translation="MFENLTERLSGVIKTLRGEARLTEENIQAALREVRMALIEADVA
                     LPAIKDFIERVKQKAVGQDVLASLSPGQALIGVVHQELIAIMGGDKAELNLSTSPPAI
                     ILMAGLQGAGKTTSSGKLAKWLIDKKKKVLLVSCDIYRPAAIDQLELLAKQVGADFFP
                     VQTGQKPKEICSAALDYSRRNHHDVIIVDTAGRLGIDEFMMNEISELEALLKPIETFF
                     VVDAMQGQDAVNTAKAFADALPLTGVILTKLDGDMRGGAALSVRHVTGKPIKFVGIAE
                     KLTGLEPFFPDRMASRILGMGDVLGLIEDAQRSSDQKEAEQLMKKMKSGKGFDLNDFK
                     MQFQQMKKMGGMTAMLEKLPHQLSQAAQNVKVDDRIINRNEGIINSMTQLERTKPELI
                     KASRKRRIAAGSGVSVQEVNRLLAQFEQAQKMMKMVNKGGMAKMMRAMKGILPQFR"
     gene            15773..16621
                     /gene="ccsA"
                     /locus_tag="PK522_04040"
     CDS             15773..16621
                     /gene="ccsA"
                     /locus_tag="PK522_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNC40935.1"
                     /translation="MTGTLVYILAFLFYTFAGWYFWQTRWKHQSIYPVNQGNDPSRAN
                     WQAHLIMLVPIIFHGLSLSQSIFIETGLSFGVGNAISSIVWLTGLIYWFTGFFPRLQG
                     LQNLFAPIAIAGAIAVLLPLLIPSVRPLANTEMTAFKMHLLVAMLAYSLFTIAALHAL
                     LMTIQEYRLHHPTLSPLLTNLPPLLAMEKMLFHIIWTGFTLLTLTLFSGIIFSQEVFG
                     RSITFSHKTLFGFISWGIFAALLTGRKLYGWRGHTAIRWTLVGFIILLLAYIGSKFVL
                     EIILNR"
     gene            16694..>17162
                     /locus_tag="PK522_04045"
     CDS             16694..>17162
                     /locus_tag="PK522_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40936.1"
                     /translation="MSSLLSNVVSLQGVTQSRLVAIVILVSFILGACVQPQPKPDLAT
                     QTTANPSQPTELEPIPIPEKLPCAKQCEAEINRLKKQLKEKGDLLRSYQAQLKDQKPP
                     NSENVSEATQAQTKLHRLASQPSAASKIAEVEAETENLKQSLLINADRSDQRLF"
BASE COUNT         5118 a         3961 c         3458 g         4625 t
ORIGIN      
        1 gtcagcttat gattatggtt atatcgaaaa cgcgcaatcc aacacgaata aagtattatc
       61 caattctaca gtaaccctaa aagattaatt tcatcgcgat aggatatgat gagataaaat
      121 cttactacta aattttaata tgactttatt agatattatt ccaccgaata atattttggc
      181 gtaatcggct gcgccattac agccgtgctt ttgaggagag attctaaatg atgatgcgca
      241 agctaactgt ttcgctatta tcaatcggtg taatcatggc atcgatgcat gtcactgctg
      301 ctaacgaacc gattcaacct atcaaaccag taaagccaga aaacgcagaa ttggttgaat
      361 taggaaaaat gctatttttt gatcccagat tatccaaatc tggtttcatt tcttgcaatt
      421 cttgccataa tttgagcatg ggtggaaccg ataacatcca aacatcaatt ggccataaat
      481 ggcagcaagg tccaattaac gcacctaccg tattgaacgc aagcatgaac ttagctcaat
      541 tttgggatgg tcgtgcaaaa gatctaaaag aacaagcggg tggcccgatt gcaaatccag
      601 gcgaaatggc ttcaactcat gatatcgcag tgaaagtaat caactctatg ccgcaatatc
      661 aagcgcagtt taaaaaagcg tttggctcag atggcgtaga tattgatcgt gttaccacgg
      721 caatcgctgc ttttgaagaa accctagtta ctccaggttc acgttttgat aaatggttag
      781 aaggtgataa aaacgcctta tccaaagaag aacaagatgg ttataacgtg ttcaaaacta
      841 gtggctgtgc aaaatgccat aatggtcctg ccgtaggcgg aaactctttt gaaaaaatgg
      901 gcgttcataa gccttatgaa accaaaagca ctgctgaagg ccgtaaagga gtaaccggta
      961 aagaagaaga caatttacat ttcaaagtac ctactttgcg caatgtagaa ctaacctatc
     1021 cgtacttcca cgatggtggc gcagctacgt tagaagaagc ggttgatacc atggctaaaa
     1081 tacaattgaa caaagaattg agcaaagcag aaattgctaa tgtcgttgct ttcttaaaaa
     1141 cattgacagg taagcaacct gacttcaagt taccaatttt accgccttct aacaataaca
     1201 caccaagacc tgagcctttt gacaagtaat agcttgttcg actgagtctt taaaaacaac
     1261 tgtcgtccat taataaaggc cacacaataa tttaattttg tgtggccttt ttatgtgctc
     1321 ctttaataac cattaatgca catgatcatc cggcggtggt tttatttttt cttcctgatt
     1381 caatatcgtg agttgcttgg ttagaacgcc tgtaatcaac caaccgaagc catcaaactg
     1441 actaacccat cgagcgcgta gatacccaaa acgatcaatc atgtattcaa tatgctctgg
     1501 agtcatccct ttcctgagta aatctggaac tgccatcgtt ctgcgaaata ataaataact
     1561 atctctaatc tcagaccaac cttcagttat gactgggaat gggacgatgc gcgttactgc
     1621 tgccatttct tcctcagtga gcgccctatt cgggattaat agtatttcag tgtgaaaatc
     1681 atttttcaac atctcgtaga tatcacttaa ctgaaataat cgctgttgcg attgcggcca
     1741 tgaaaacaac accagtaata cattactttt ttcacggaag tcttttaagc tcccaccaga
     1801 gccatcatgc gcaaaataag aaaaaacagg cggtgctaga aaaggctgat tgggtgtcac
     1861 taagctaccc aataaccttc ccatataacc tctggaaagt gcatgaagat aattgacaac
     1921 atcccaacgg ttctcatccg ttaagacggc tgaaaaacct ggcatactgg tgccctcaac
     1981 cccatacgat aaccaatgat aaaaattacc gacagtatgt ctagctgtat gcgttttggt
     2041 aagtaaatca gtgggatccg gtggaaactc acccgtttcg ggatcagcaa caaaaagctc
     2101 aggcgctgta cccgtgccct ttccctgagg accatgacaa ctgacgcagt gttcagcaaa
     2161 tagtgtagcg cccttcgata ttgaaattgc gtcaaatgga atgggtgttt tttgataggt
     2221 ttcgggatag gcttcaatga ttagcggggg caacgcaatc cccagtgaaa caagacccat
     2281 gataaccgga agaatgactc gctttgactg attccatcga ttcagcgttc ccatggcggc
     2341 gatccctatc gccaggatga ataataaagt acccgcccag aaaaagtttt gaactccagg
     2401 ttcgtcccaa gtcgcatcaa tcgagagccg gaaaggataa ggccaatgat cgatgatcgc
     2461 atgtttacct ggaagcgtat tggctaataa ggtagcgact aataccagga tgagtgcaag
     2521 cagaaattca atcccaaccc atttttttaa ctgaccaatc gctacacctg cacgcacatt
     2581 ttcttggata aatagcggta accaaacaga acggattcgg taggcgatcc ataaaataat
     2641 gcccagtatc gataatttga gattaagcaa ccatccgtaa cgactagata ccaatgtatg
     2701 ataatagtcc ccaatcatac gatcggctgc catccatcct gtgaggaata gcagtatcat
     2761 gacaggcaac gcaagtactg aaaattgttt caaaaaatcg gcatgcagca aaagcgtacc
     2821 ggcttgcgaa ttgttaagcg catcacgaat aatataaata atcgcaggca aagtcccaaa
     2881 ccaaaccccg gctaataaga tatgcaatgt atagggagtc actgaagcaa aagaaatttc
     2941 ctcagcgcct gcatgactaa ccaatgatcc cacagtcaaa ggtaatgcgg cagttactgc
     3001 aagcagcccg taatgccatc gaacgcgatg catacgttta aggcgaagaa cgataaacaa
     3061 caatagcaaa gccaacccaa accgcgctac ccagaggtgt cccactcggg tttttagcac
     3121 aatctcccac caagcattta gatcccaggt attaatcgca tcacccgtag cctgacctgc
     3181 agtgctagct aaaataccaa tcaatcccaa taaaacgata cctactaacc aaggaaataa
     3241 ttgctctaat tttctgaccc aacccagttc cggtagtttt tttccaaacc aaatcagtgt
     3301 caaaaaaaca caactaccaa ataaaacaag attggcggtt agctgtaacc accttaccaa
     3361 cgcagaaaaa aattcgatca ttgctttaca acattaaaat cattgacgaa actcggggat
     3421 aaactaaacc ttgagcagaa cgatagatac cagcttgata cataagataa aatgaaaaaa
     3481 cagaaaggca ctgcaaaact gccctatacc atagcgtata accgcctttc attcaagtaa
     3541 gccatactaa ggcttaagaa gtcaaaaacc aatgattcaa tttgctaccc cggatgtctc
     3601 attgcagtaa atcgattatg attgatcgca atgataacca ctgagacctt ctatgagtga
     3661 ccctattatc acttgcccca actgtcatgc tgccattcat ttgaccgaat cgcttgcggc
     3721 cccactatta gctgcaacgc gtcaacaatt tcagcaacaa ctcgcgcaaa aagatgaagc
     3781 aatctcccaa cgtgagcagg caatccgcca acaagaacaa ctcctgctcg aagccaaacg
     3841 caatgttgat cagcagatta ccgagcaagt tgctgcgcaa ctcaaggcag aacgcgatca
     3901 cctcatcgcg gatgaagcca aaaaagcaaa attggcggtt gccgtagagc tcgaaaataa
     3961 tctacgtgaa ttggccaatc tcaaggaatt actacaaacc cgcgagcaaa aacttagcga
     4021 agcacaagcg gctcaagccg atttgttaaa aaagcaacgc gaactcgatg ataccaaacg
     4081 tgaactcgaa ctcaccatcg aaaaacgggt acaacaaaat ctaaccacaa ttcgcaacca
     4141 agcgcgccaa gaagcggaag aaagcctcaa gctgagagtc atggaaaaag accaaaccat
     4201 cgctgccatg catcagaaaa tcgaggaact taaacaaaaa gctgaacaag gctcccaaca
     4261 attacaagga gaagtacaag aacttgaact ggaagcactg ctacgcagca aatttccaca
     4321 ggactccatc gagcccgtcc ccaaagggga gttcggtggc gatatcttac acgcagtcat
     4381 cagccccagc ggtctaccga gcggattaat tttatgggaa tcgaaacgca ctaaaaattg
     4441 gagcgaatct tggttaagca aactgcgcga tgatcagcgc aacgctaaag ctgagctttc
     4501 ggtattgatt agccaggcat tacccaaagg catcgaaacc tttgatatcg tcgatggcgt
     4561 ttgggttgtc catccccgcg ccacattgcc agtcgctact attttgcggc acatgctatt
     4621 gcaaatgagt cgcaccaaac aagtcagtga aggccagcaa accaaaaccg agctgatgta
     4681 tcaatacctg actggcccac gtttccgtca acgcgtagaa gcgattgtgg aagcattcac
     4741 caccatgcaa accgatctcg ataaagagcg taaagcaatt ttaaaacaat gggcaaaacg
     4801 tgaagaacag attacacatg tcatggaagc aactgtgggc ttatacggtg atttacaggg
     4861 cattgcggga aaatcactgc aagagattga aggattggag ctggaatatt tagagatcaa
     4921 ggattaggac atcaaaaaac ctactcactc tgatctgatc agccatcaat tctatagcac
     4981 gctattcata caatgtgaat tagtttgatc agcaattatt tttaactgca gttttacaaa
     5041 aaagataaac cctacggata cgccttaact tcaataccca gcgccttaat tctatctgct
     5101 aattgggcta gccactgatc ggatactttc gacaaccgat ccgcttccac ttgcatcggg
     5161 ggacgtgcca acccgtaaag taatacgcct tgtaacggga tttgagcatc gcgggcttgt
     5221 gctataaact ccaagtaagc gtcaacctca ctttcacagg gcggttgatt gtcgatttgg
     5281 agcatgcacg tttggatcca ggtggggcac agcgttgtgg ctattttcaa atgacgctta
     5341 atcgtaccat tactgatgct tgctccatta atcaattgtc tgcggtctgg aatggcgcta
     5401 tcgaatttaa accaaacttc gccattgagc gtggccatcc gcgccaatcc ccgctgtacc
     5461 gcaacccgat tgattaaact cccattggtg atcaacacca gcttgatcga ttcagctaag
     5521 gcaaaatcgt gtttcacacg tgcgatgact tcgataacct cgcaaaactc tttggcgctg
     5581 gtgggttcac cgttacccga taacgcaata tcgttaactc gctgacattc gggtggcact
     5641 tgctgggcca tgaaatctcc ttccagcaat tcgcgcagaa agatcctcaa ttcctgttct
     5701 agcaacggca aatcaatttt cggtgcagat ccacgcgtta ggttcggcac ttgacaatag
     5761 atacagcgcc aattacaggc gttattgggg ttgagattga tgccgatcga taccccaccc
     5821 gagcgacgcg aaattacagg gtaaacatag accatgccga caatatcgcg gcgatgatct
     5881 tgcgttgtta agattttcgt ttgattggca atgatataat ttccctatgt taattacacg
     5941 tcgtttagaa ttcgatgccg gtcatcggat ttccactcat accagccagt gtcggcattt
     6001 acatggtcac cgctatgtaa ttgaggtcac cttatcgggg aatattatcg ctgaggaagg
     6061 tgtaccagaa caaggcatgg tcatggattt ttctgagatc aaacggattg tacgcgagca
     6121 aattgtcgat ccgtgggatc atgcctttct cgtctataaa aacgatacga tggtcgtcga
     6181 ttttctgcgt tccttagaaa accataaaac ggtcattctg gattcccaac cgactgctga
     6241 aaacttagct aacgttgcgt ttttgatttt agatgaagca ttccagaaaa attatggaga
     6301 tcggctgaga ttagaacggg tgcgactgta tgaaacccct aattgctggg cagatgcgac
     6361 ccgagcgcat cacgggattt aattattttt tattggattt atttgtctat gttcattgat
     6421 tcacactgtc atctcgattt tcctgatctt gctgcaaact tgcccgaatt actcgataac
     6481 atggtacaaa accaggtaag ccatgcgctc tgtgttagcg ttaatatgga agattttcct
     6541 cgagtccgcg cgcttgctga aaactacgac cacctctatg cttcagttgg agttcacccc
     6601 gattatgaaa acatcactga accaaccgtg gctcagctcg ttgagctcgc tcaacatcct
     6661 aaagtgattg ccataggtga aacagggtta gattattttc gattaaaagg agatctggaa
     6721 tggcaacgcg agcgcttccg ccgccatatt cgtgcagcca aagcgactca aaaacctttg
     6781 atcatccaca cccgcgaagc agcggaagat accttgcgca tcatgcaaga ggaagacgca
     6841 gcaacgattg gcggtgtcat gcattgcttt acggaaaatt gggatgttgc acaacgcgct
     6901 atcgagatga atttttatat ttctttttca ggcatcgtca cgtttaaaaa tgccctaatg
     6961 ttgaaagaag ttgctcggaa agtgccctta aaccgcatgt tgatcgaaac agattcgccc
     7021 tatcttgcac ccgttccaca ccgtggaaaa accaatcaac ctgcatttgt taaacatgtg
     7081 gctgaagaaa ttgccaaact ccgcgaaaaa agcctcgatg aaatcgcgac cgcaacaacg
     7141 gataacttta ataccctttt tcgcttaccg cacacatcgc tcactaccca ttaacttcaa
     7201 agtaagcccc gctccgcaaa tgaagtaatg ccttccgaac cgacaataaa gtgatccagc
     7261 actttgactt ccacataggc cagtgcttgt ttcaaagatt gggtcagtac ttcatctgct
     7321 tgactcggtt cggcatgtcc tgaaggatga ttatgcgcaa aaatgatggc tgcagcattg
     7381 tgaaataacg cacgtttaat gacttctcgc gcataaacac tcgtttctgt cagtgttcca
     7441 ctaaataatt cttcactttg gataatgcga tttctggcat ctaaaaagag tcccataaac
     7501 acttcatgtt gacgatggac caagctaagt cgcaaaaatt gccgcacttg ctgcggagaa
     7561 gtcatgatat cgccccgctt taaacccgtt tcgagcgcac gctgcgccat ttccagcgtt
     7621 gctttgagtt gtacgtattt ggctacaccc aatcctggta tttgacaaaa tgatcgctga
     7681 tctgcgctga ataactcggt caaactccca aaatgactca gcagttctct tgcaagatca
     7741 accgcactct ttccagtaat accggtgcgt aagaaaatag cgagtaattc tgaatcggaa
     7801 agactagcgg gcccatagtg caataatttt tcacggggac gttctagctc aggccagtca
     7861 ctaattgcca tcatatttct ccatcgcgtt aaccatgagc catgtaaacg gtcattccaa
     7921 ataaaactac ataaagtcaa aaatcatcgt aatatgatca gtcgttgtat tattaaggct
     7981 atgtcataac caatctcaat ttgttaattt gcaagcagga tggattcgat cagcgcgctc
     8041 accggtaaaa aactgatact cggcataacg ggaggtatcg cagcgtacaa aactgctgaa
     8101 ctcgctcggc ttttagtcaa agaaggggcc atcattcaaa cggtgatgac ccaagctgcc
     8161 tgccgattca tagggccaga aaccctccat gcgttaacag ggaatccggt ttacaccgag
     8221 ctttggggag attccctaca aaattccatg gctcatatcg gattatcccg cagtagcgat
     8281 gcgatactca tcgcaccgac cagtgctaat ttcatcgcga aactcgcaca cggtgtcgcc
     8341 gatgatttgc tttctacact ttgcttagcg cgggattgcc ctttgatcat cgcaccagcc
     8401 atgaataagc agatgtttga gaatccagcg acacagcgta atctcaatac cttgcgagcg
     8461 gatcagataa ccatctgggg tcctgccaat ggtgatcaag cctgtggtga aaatgggctg
     8521 ggacgcatgc ttgaaccgga gcaattacaa caggctttac aggttttttt tgagcctaaa
     8581 tggcttgatg gaaaaaaggt tctaattacc gcaggcccaa catatgaagc catcgatgcc
     8641 gtgcgcggta tcatcaattt gagttcgggt aagatgggct atgcaatcgc aaacgcagcc
     8701 agcaatgcag gtgctacagt taccctcatc gcagggccgg ttcatttacc caaaccatcg
     8761 gtacaaaaat tcattagtgt taccagcgca acacaaatgt tagcggctgt taaaaaagaa
     8821 gtaagcaact gcgatatctt tatcagtgtc gcagccgttg cagattcccg ccctcaaccc
     8881 taccagccac aaaaaataaa gaaagccaac cagccattaa cgcttgagtt agttcctaat
     8941 cccgatatcc ttcactatgt ggctcattta aaaaaaccac cgttttgcgt tggttttgca
     9001 gcagaaacgg aaaaactcga tcaatatgct caaaagaaac gacaacaaaa gcgattaccc
     9061 ttgatcgtag caaatcttgc ccaacaagcg atgggggctg acgaaagtac gttaatcttg
     9121 ttcgatgata acggtaaaca tgttttaccc caggcctcaa aaaccgaact cgcccgccag
     9181 ctcattaaac atattgcaca gttatttgaa aaacaaaatg cctgagtctc tacatgattt
     9241 tgcacgccac tcatggaaat ctggagttaa acatatcagc atcaacccca ttcttccaat
     9301 caaaaaaacc tcaaattatc gctaatttaa gtctttgaat tgagactggc tatgggaaaa
     9361 tcattgcttg tatgctcaat gattttctat accaagcagt aaaggaattt ttgtgaccat
     9421 ggtagttact tcgtatcgaa aacccatctg tgccagtaaa acacattggc agttatcact
     9481 ttttctgttg ttagcgcctt ttatcaattc tgcatttgct gcacagaata tccagactat
     9541 acgcaatctt gctgatgcct cgcttgagga attgatgaat gtcaaaattt ctactgtttt
     9601 ccggaaaccg caaacattgc aagaaacagc cgctgcggtt tttgtgattt ctcaagaaga
     9661 tatccgccgc tccagcgcca atagcattcc tgaattattg cgcatggcac cgggtttatc
     9721 agtagcacgg attgattcca acaaatggtc agttacttct cgtggttata gtggccgctt
     9781 tgctgatgat ctcctcgtta tgatcgatgg acgtactgtt tattcaccgc tgtttgcggg
     9841 tacattttgg gaaacgctcg atctaccctt ggaggatatt gatcgcatcg aagtcattcg
     9901 ggggcctggt ggaacaattt ggggcgctaa cgctgtcaac ggtgccatta atatcatcac
     9961 caaacatact aaagcgactc aaggcaattt aatcaccact gggggtggta gtgaagagcg
    10021 agggttcgct actttccgcc atggtggacg tgtgggggaa aatctttttt accgttttta
    10081 tgctaaggga tttacccgtg ataacagctt caatgcaagc ggtacaaacg acagttggcg
    10141 catgggaacc ctcggttttc gggccgattg ggatatcaat accaataatg ccgttacctt
    10201 tcaagcgcaa tactatgctg gaaaggctgg ccagaatacc atgcttctca accctactga
    10261 acctgagtca gccattacgc gccctgaaga tgccaatctc gctggtgggc atgcattaat
    10321 gcgctggcag cgaacgactg gagagcgttc aaatacggca ctacaagttt attatgatcg
    10381 cacatctcgc aatgaattgt cttttcggga aaccagacaa accgttgaca tcgatttcca
    10441 gcaccgtttt ccattacaaa ttccttatat tccattccag ccggatctga tttggggagg
    10501 aggctatcgc tggactaccg atcatttagg aattaactta ccgatttcct tcgatccagc
    10561 aaaacgtaac ttacagactg gcaatgtctt catgcaaacc gacataccgc tatttgaaga
    10621 tcggctaaaa ctcaccacag gaattaaatt tcttgataac acttacgcac attcgaattt
    10681 cttacctaat gctagactat tatggacgcc agatgacaag caatctgtgt ggttatccgc
    10741 aacacgttcg atccgtttac cctcgcgctt cgaacgcgat ggcaaccaac tcatcagaag
    10801 tggcgccgaa tttattcgtt tgatcagtaa tcctcatctg cttgcagaaa cactatgggg
    10861 atttgaaacg ggctatcgac gtcaagttac ttcaaattta tcagtcgaca tcgcaggttt
    10921 tttcaatgac tataataatt ctaccagcga acgagaaaca accgcagaaa caattcaact
    10981 taccaatcaa cgcaagactc aaatctttgg atttgagatt tatggagaat ggcgtgcctt
    11041 tgataagcta cgcttcatgc caagctatag tcacttacaa gtaagaaatc atgtgcctgc
    11101 ggatcatgag tcggaatccg gcgaagatcc caagcatcaa tttacactcc gcacacaact
    11161 tgatttaacc aagaatattg agctggatgc gttcttccga cacatcgata aattacctgg
    11221 tttggaaata aagagttacc aagcgctaga tttacgatta tcgtggcggc caagatcaga
    11281 tacggaatta accctggttg gacagaattt attgcaatca caccatcagg aattttcccc
    11341 cgaagtcatt caaaccatgc cggtgcagat tcaacgcggt gtatttggta aagttacttg
    11401 gcgattctga tcaaatccat tgtgaaagcg atgctcaagt tactaatgca atatttttac
    11461 aacttaggca gcttgttttc acataaaaaa ccaagtgcaa tcctttactt attgctcatt
    11521 ggcataagcg ctattctacc caagcctttg caagctgagg aagtatcgga atatggctta
    11581 aaagccctct ttttgtataa cttcgcaata tttacctcct ggcctggtag caatatcgat
    11641 aaatttaatc tgtgcattta tggcgataat ccttttggtc aagatattaa ttttttaatg
    11701 aaaagtaaaa aaattaatga gcgcacgatt gacattcata gaatcgataa tgtgagccaa
    11761 cttgatcagt gtcagcttgt ttttatttcg cgtcctatga tttctaactt agccgatgtg
    11821 attactacag tcaaagacaa gccaatttta accatagctg acagtcctgg cgcctgtcat
    11881 cagggcgtga tactaaatat gaatgtcaat aatgagaaag tcacctttga agctaattta
    11941 atgatggcaa aaaaagctgg cttaaattta agctcacaat tattacgcct cgccacagaa
    12001 atttatcaat aaatagccaa acacatacat aacaacttaa atcacctcag aaattctcaa
    12061 gacaaagtga tttaagcaat gagaaaatta attctccatt atcgaaatca tgttaagtca
    12121 ttacaagatt aatttgatat gatcttcagt tgtaaactac tcattctaac ttttagaatc
    12181 cataaaataa tttgagtatg tgaccagtca ttactaacgt taatttcttg gaggctgtat
    12241 gagagtaata ttatgcttag tgctcatagt aagttcttat gcttatgcac aaagtgggca
    12301 cgatcatcaa cataaatcac aaaaagaaga cacccagcag ggtggtatgc aaggtcatgg
    12361 tgatggccat catggtatgg gcggtatgat gatgcatggt ggacaccaca aattagatgg
    12421 tgctacatta aagcatttct tcgaaccact tcccgcatcc atcatcgatg aaaagaaaaa
    12481 tgccacgctg attgcgcttg gtaaaaagct atatcttgat ccaaggcttt cgatcaatga
    12541 tacgatttct tgcaattctt gccatcaatt aaacaatttt ggtgttgata gccaacctac
    12601 ttcacccggt catgatggca cacgcggtgg tcggaattcc cccacaacat ttaacgctgc
    12661 acttcatatt gctcaattct gggatgggcg tgccaaagat gttgaagaac aagcacttgg
    12721 tcccattctg aatccaatcg aaatgggcat gcccaatgaa gaagcggtgg tcaacaagct
    12781 gaaaaaaatc gatgagtaca aaactctgtt cgcgaatgca ttcaaggacg aaaaagaccc
    12841 tattcaatat aaaaatattg gcaaagcgat tggtgcattc gagcggactt tattaacccc
    12901 ttcgcgtttt gacgattttc tcaaaggtga tgagaatgca ttgaatgatg cggaaaaacg
    12961 cggattgaca aaattcgtcc acatgggatg tgcaaattgc cataatggcg tagccatcgg
    13021 tggtaattca tacaaaaaaa ttggcctggt tgaaccgtat gaaaccaaag atctcggcag
    13081 atatgaggta accggtttag aaactgataa gaaagtattt aaagttccga gccttagaaa
    13141 cattgcccaa actggccctt atttccatga tggttctatt gccacgctag aacaagcgat
    13201 tgaagaaatg gcagagcacc aacttggtcg cgaagtcggc cctggattta ttgaagatgt
    13261 gaaagcattc ttaggttcat taaccagtaa aagcaagtaa tttaagtacc accttactga
    13321 aacattacct tactccccac aaattagttg tggggaattt cttgctactt cagcatatac
    13381 tgctgagttt gactcatgaa aatcgatatc aaaattctgg acacacgact acaaactcaa
    13441 cttcctagct atgccacccc cggttcggca ggattggacc tgcgcgcctg caccgagcaa
    13501 ccattaacag tccaccccgg cgaaacacaa ttgattccta ccggcattgc catccacatc
    13561 gaagaccctg ggtttgctgc ggttgttcta cctcgttctg gtttaggaca taagcacggt
    13621 atcgtcttag gtaatttggt tgggttgatc gattctgatt atcaagggca gatttttgta
    13681 tcatgctgga atcgtgggca aactgaattc ttgctcaatc ctttagaacg aattgcccaa
    13741 ctggttgtta ttcctgtcgt acaagcgcag tttaatatcg tagaaaactt tgcaatgagt
    13801 catcgcggtg aaggtggttt cggtagcact ggccgatgat tagatctaat ctatttatac
    13861 tcagcttttg cttgggtttg acactgagtc cgctattact ggctgagaat ctggtagttt
    13921 cgttaacgat tggcggtcat cccctcaatg cggaaatcgc tcatacctat gatacccgtt
    13981 ccaaagggct catggaccgc gccacgctgc ctgaaaacca gggtatgctc tttatttttc
    14041 ctaaaattga cttctacagc atgtggatgc tcaatacgtc gctacccttg agtgttgctt
    14101 ttattgatga aaaaggcatc attctcaata ttgaagcaat ggagcccttt tcaacagtag
    14161 cacacgcttc gatcaaaccg gcaaaatacg cgcttgaaat gcatcaagct tggtttacaa
    14221 gcagaaaaat tactgccggg cataaaataa tcggacttga aaatgtaccc gatgcaatcg
    14281 aatgatacgg tgcttcctta cctccaacaa aattaacgga attgcggaag aatgcctttc
    14341 atggcacgca tcatcttagc catacctccc ttattaacca ttttcatcat tttttgagct
    14401 tgctcaaact gagctagtaa ccggttgact tcctgcactg aaacccctga accagctgcg
    14461 atacgccgtt tacgcgatgc tttaattaat tctggtttag tacgctccag ttgcgtcatg
    14521 gaattaatga ttccttcgtt gcggttgata atgcgatcat ccactttaac gttctgtgca
    14581 gcctgactca attgatgcgg taatttttca agcattgcag tcattccacc catctttttc
    14641 atttgctgaa attgcatttt aaaatcattc aagtcaaagc ccttgcctga tttcattttc
    14701 ttcatcaatt gttcagcttc tttctggtct gaactacgct gggcatcttc aatcaaaccc
    14761 agtacatcac ccattcctaa aatacgagaa gccattcgat ctggaaaaaa tggttccaag
    14821 cccgttagtt tctcagcaat ccctacaaac ttaatgggct taccggttac atgtcgaact
    14881 gatagtgcgg ctccacctct catatcccca tctagcttag ttaagatgac gcccgtcaaa
    14941 ggtagtgcat cagcaaaagc tttggcggta ttaacagcgt cctgaccttg catcgcatca
    15001 accacaaaga aagtttcaat gggtttgagt aacgcctcga gctcactaat ttcattcatc
    15061 atgaactcat cgatgcctaa acgtcctgca gtatctacga taatgacatc atgatgattt
    15121 cttcgcgaat agtctaaggc tgcactacat atttctttgg gtttctgtcc agtctgtact
    15181 ggaaaaaaat cagcacctac ctgcttagct aataattcca actgatctat cgcagcggga
    15241 cggtaaatat cgcatgagac taacaatact tttttcttct tatcgatcaa ccatttggcg
    15301 agtttcccac tactggtcgt tttacctgca ccttgtaacc cggccattaa aataatagcc
    15361 ggcggcgatg tgctaagatt gagttctgct ttgtcgccgc ccataatggc gatcaattct
    15421 tgatgcacta caccaatcaa cgcttgcccc ggcgaaaggc ttgcgagaac atcttgacca
    15481 actgcttttt gtttgacgcg ttcgataaaa tccttgattg caggtagcgc aacatcggcc
    15541 tcgatcaagg ccatgcgcac ttcccgtaaa gcagcttgaa tgttctcttc ggttaacctt
    15601 gcctcaccac gtaacgtttt aatgacaccc gataaacgtt cagtaagatt ctcaaacata
    15661 gtgttctctc aaataattat tcattattaa tttttacgct ttaaatttag gccgtcccat
    15721 tgtagactgt gcacaccgaa atctatggtt agtaaactat actctacttc gaatgactgg
    15781 cacattagtt tatatcctcg ctttcctatt ttacaccttt gcgggctggt atttctggca
    15841 aacacgatgg aaacatcaat cgatttatcc agtcaatcag ggcaatgatc catcgcgagc
    15901 aaattggcaa gcgcatctca tcatgttggt tcccattatt ttccatggat taagcctatc
    15961 ccaatcaatt tttattgaaa caggccttag ctttggcgta ggtaatgcga tttcctcgat
    16021 tgtatggttg actggtttaa tttattggtt tactggcttc ttcccacgtt tacaaggtct
    16081 acaaaatcta tttgcaccga ttgcgattgc tggagcaatt gctgtgttat tgcccctttt
    16141 gatcccctca gtacgacccc tagccaatac cgaaatgact gcatttaaaa tgcatttgtt
    16201 ggttgctatg ctggcttata gcttatttac aattgctgca ctgcacgcgc tgttaatgac
    16261 catacaagaa tatcgtttgc atcatccaac actatcaccg ttgctaacga atttaccacc
    16321 gttgctcgca atggagaaaa tgctatttca cattatttgg actggtttta cactcctaac
    16381 actcacatta ttcagtggca ttattttctc acaagaagtc tttggacgat cgatcacttt
    16441 ttcacataaa acactttttg gctttatttc atggggaatt tttgctgcac tactgaccgg
    16501 gagaaaactg tatgggtggc gcggtcatac tgctatccgc tggaccttag taggctttat
    16561 cattctgctg ctggcctata tcggcagcaa atttgtgcta gaaattattt tgaatcgttg
    16621 aaatgatttt cactattgcg ttttaaactt tccaatcatt tcgctatgaa tttttaatca
    16681 tcggattgga atcatgtcga gtctacttag caatgtcgtg tctttacagg gtgtaacaca
    16741 atctagattg gtagcaatcg ttattttggt gagttttatc ttaggtgcct gtgttcaacc
    16801 ccaaccaaaa ccagatttag caacccaaac aaccgctaat cctagtcaac caactgagtt
    16861 ggaaccgata cctattccag aaaaattacc gtgcgccaaa caatgcgaag ctgaaatcaa
    16921 tcgactgaaa aaacaactta aagaaaaagg tgatttgctg cgtagctatc aagcacaatt
    16981 aaaagatcag aaaccaccca atagtgaaaa tgtcagtgaa gccacgcagg ctcaaactaa
    17041 actacatcga cttgcttcac aaccgagtgc tgcttcaaaa attgccgaag tagaagcgga
    17101 aactgaaaat ctcaaacaat ctttgttaat caatgcagat cgatcggatc agcgactatt
    17161 ta
//
LOCUS       DAOQLJ010000034        17006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1210_length_17006_cov_5.994986, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000034 DAOQLJ010000000
VERSION     DAOQLJ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 17006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17006
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1210_length_17006_cov_5.994986"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PK522_04050"
     CDS             complement(<1..498)
                     /locus_tag="PK522_04050"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HNC40937.1"
                     /translation="MIDRNGYRPNVGIILLNSKNEVFWGKRVKQNSWQFPQGGIKTGE
                     TPTQAMYRELTEEIGLQPNHVEILGRTREWLRYNVPECWIRRECRKSYKGQKQIWFLL
                     RLLGRDTDVSLRKSPHPEFDAWRWNQYWVELDSVVEFKRQVYRQALLELSRLLGRDIG
                     SAYDYG"
     gene            728..2440
                     /locus_tag="PK522_04055"
     CDS             728..2440
                     /locus_tag="PK522_04055"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNC40938.1"
                     /translation="MRVSNFFLSTLKEAPAEAELISHKLMVRAGLIKRLGSGLYSWMP
                     LGLRVLRKVEQIVREEMNRSGAQELLMPAVQPAELWQETARWEQFGPQMLKIKDRHEN
                     DYCFGPTHEEVITDIVRREIKSYRQLPINFYQIQTKFRDEIRPRFGVMRAREFIMKDA
                     YSFHVDQASLEKTYQIMYETYSRIFTRIGLDFRAVAADTGAMGGSSSHEFHVLADSGE
                     DAIAFCPHSDYAANVELATSLPGNSMPSEAPTGAKNKISTPGIKTCQEVAEFLGIPIQ
                     KTVKALAITADEQFFLLLLRGDHQLNETKVRKIPLLTNFEFASEERIITETGCKPGYI
                     GPIGMEKYLIADRSVLEMSNFVCGANEENFHFTQVNFGQDLKVPDRCFDLRNVVAGDL
                     SPDGKGQLEICRGIEVGHIFQLRTKYSEKMKATFLDDAGQIKVMEMGCYGIGVSRVVA
                     AAIEQNHDAAGIIFPTAIAPFEVAIVPIGYHKNQSVQQAADQLYQTLSQAGVEVFLDD
                     RDERPGVMFADMELIGIPHRIVIGERGLKEGAAEYRGRRDQAAQMIPIAEADHFLRAK
                     VCGT"
     gene            2428..3015
                     /locus_tag="PK522_04060"
     CDS             2428..3015
                     /locus_tag="PK522_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNC40939.1"
                     /translation="MRNLALIILLLKPCWIFAGAQLYEPLSASTRTVLQKAISDQPIA
                     QFVTLPDAESRRWLAAMSARMEKYMPDQNEREGFLTTVHYEAMRAGLDSQLVLSIIHV
                     ESRFRKYAISSAGARGYMQIMPFWIDTIGSKEHNLFHLRVNLRYGCTIFRHYLDLEKG
                     DYFRALGRYNGSLGSATYPDLVFSAWQKNWFFSKD"
     gene            complement(3049..4119)
                     /locus_tag="PK522_04065"
     CDS             complement(3049..4119)
                     /locus_tag="PK522_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC40940.1"
                     /translation="MESLLKLTQIHQSYHQQEIIKRLSLELKKGEIGCLLGPSGCGKT
                     TVLRCIAGFESISSGEITINGKIVCNADFNLPPEQRRIGMVFQDYALFPHLSVSANIG
                     FGLQQYTKAERTHRINELITTVGLTEASDRYPHELSGGQQQRVALARALAPRPDLLLL
                     DEPFSNLDISLRERLSLEVRDILKDQHTTAILVTHDQTEAFSVADVIGVMHQGEILQW
                     DTAYNLYHHPTNRFVAQFIGQGTFVPGHLISPEKIQIALGIVENPDYSDYFEQSEHTT
                     NEAVDVLLRPDDIVYNEASALQATVVNKAFRGANILYTLKLSSGQTLLSLIPSHHNHS
                     IGESIGIQLAADHVVVFSREKM"
     gene            complement(4125..5723)
                     /locus_tag="PK522_04070"
     CDS             complement(4125..5723)
                     /locus_tag="PK522_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNC40941.1"
                     /translation="MNFWRLAPFCVASIVLIPIIVVISSIFTPADDIWHHLAETTLPS
                     VLLNTFWLCLGVVLGAIILGTSLAWLTAIYDFPGRKFFSWALLLPMAIPAYVTAFVVL
                     GLFDYTGPIQTYLRSWTGAESNWIPDFYGRSGAILVMVLAFYPYIYLLARNAFITQGK
                     RLIEAAQSLGLSRKQGFFKVALPMARPWIIGGAMLTLMETLADFGTVSVFNYDTFTTA
                     IYKAWFSLFSLSAASQLASLLIILVFIIIIIEQQLRARMRFAESKSSRTTFRIPLLGW
                     KRLAACSYAFSILFIAFILPCSQLLIWAIDEINKNIDSRYIEFLIHSLTLSTIATLII
                     GSVAILLVYATRLYSDKATHIAVRIATLGYALPGAVLAVGIFISLAWIDTQLNQIILI
                     LFEIEAGFLIQGTIIAMLIAYLVRFLAVGHYPIDSAMQRVTRSIDEAARGFGLTGWSL
                     LSRIHLPILSSGIFTAATLVFVDVMKEMPITLMTRPFGWDTLAIRIFTLTSEGAWNQA
                     ALPAIALVLAGLIPIILLMRQTEQ"
     gene            complement(5819..6772)
                     /locus_tag="PK522_04075"
     CDS             complement(5819..6772)
                     /locus_tag="PK522_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC40942.1"
                     /translation="MASPLHAQEVVVYSARMEQLIKPMFDAFTTETGIKVKFTTDKEG
                     ALLARLQAEGKRTPADILITSDAGNLWEAARAGLLKSISSPILEANIPVQLRDPQGQW
                     YGLSIRARTLVYNKEKVDPSELSTYEDLANPKWKGRLCLRSSKKVYNQSLVAMMIAEH
                     GEAKTEQIVKGWVANLATEPLSDDTKAMEYVAAGRCDVTLVNSYYYGRLMETNPNLPL
                     VIYWPNQDNNGTHINISGAGVTQHSKHEAAAIKLLEFLSSEKAQNLFADVNFEFPANP
                     QIKPSPIVTSWGDFKKSPMSMIKAGELQATAVKLMDRAGYR"
     gene            complement(6985..8094)
                     /gene="ald"
                     /locus_tag="PK522_04080"
     CDS             complement(6985..8094)
                     /gene="ald"
                     /locus_tag="PK522_04080"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNC40943.1"
                     /translation="MIIGVPKETKDHEFRVGLTPGTVHALTTQGHHIKIESGAGLGSA
                     FTDDDYRLAGAEIVTYPRDAWAAQLVIKVKEPTESEYAYLRQDLILFTYLHLASNKPL
                     TEQLLATGTTGIAYETVQLASGELPLLTPMSEVAGRMAAQIGALYLQKTLGGRGILMG
                     GVPGVAPANVVILGAGIVGVNAARVTVGMGAHVTILDINHSRLKYLDDLFRGQLFTRM
                     NDSFNLNELVYQADLVIGAVLIPGGRAPWLITREMLTKMRKGAVIVDVAVDQGGCVET
                     THPTTHSNPVYEVDGILHYCVTNMPGAVPRTSTLALNSQTATYIKLLADSGLDAVRNN
                     NALQKGLNTYQGLITHTAVAEAFGLQSTAPLQALA"
     gene            8853..10622
                     /locus_tag="PK522_04085"
     CDS             8853..10622
                     /locus_tag="PK522_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC40944.1"
                     /translation="MLKYDYSLSKNEQIALFFFIIGLITVFGLLLFSNKSEERIAESS
                     THLQQDLTKQFYQQKLQQREAEILEVLKHTDPKHRRILEVELQDIQLLLQDIDNSFLL
                     LKTSINDRLKQLEVLHQAYPIQMLEQAIMALRQANDQQAIQLFRQIEKNNEHNPPLAA
                     EAAYQLSKLAEDSVRYTAGLEHAEKAIRLIPNNALYLSAAGFFSDKLAHYKKAISYHE
                     QALAILRTLHSENHPEIAAVKNDLGMAWDALGEYAKAIKFFEQALESLIQLHGENHPD
                     VAAIQNNIGMAWDSLGDYPRAIRYYEQSLEADLSHYGEDHPSIAKRRNQIDHAWESIE
                     AYHDAIQYFEAALDSDLKTYKTNHPSIARDRNDLGIAWESLGEYHKAIEYFEQALETS
                     VAIYGKHHPLIVTYLNNLGNAWDSLGKYRKAIEYFEQALETGLALHDENHPSIAVTRN
                     NLGNAWESLGELHKAISYYEQALAIDLKAYGQNHPNIARDKNNLGNAWESLGEHQKAI
                     YYFDQALSIVLQSHGENHPQVTTYRNNLGWAWYSSGHYQNAIDYYKQALVTAKKLLGE
                     DHPNTKVIQSNLRSAESKLIATP"
     gene            10752..11144
                     /locus_tag="PK522_04090"
     CDS             10752..11144
                     /locus_tag="PK522_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNC40945.1"
                     /translation="MAIEISWEAASNKTACQIQENGKTWISDIGLESGGSGQYPDPHQ
                     MLDSALGACTVLTIQIYAKRKAIPLERVLVSITHTEEAGLYQLQRNIQLKGQLTEAQR
                     DDLLRVANKCPIHKVLSGQFTIITQLEN"
     gene            complement(11189..11989)
                     /locus_tag="PK522_04095"
     CDS             complement(11189..11989)
                     /locus_tag="PK522_04095"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HNC40946.1"
                     /translation="MVLVTKCPVCQSVYRVTFPELHLHDGKVRCGQCEQAFNAFRALI
                     SIPESLIRMEDSQLALPVHEEVELPAINKHPAPSGLQSSEQAELTSASIADQADQNGV
                     AEVHTPNLIWKQVAASLALIILLCVQLIYFYRTELAIAIPSSRAILEKSCDLLQCEVH
                     LPKYSALLSLESSELRVNPNHQAEIVTLLATIRNHAPFQQAWPELRLTLTDLDDQPIA
                     SRILLASDYLNDNKLPQFFAPDSEIEIKIYFDNSELKAMGYRLELIYL"
     gene            complement(12003..12926)
                     /gene="prmA"
                     /locus_tag="PK522_04100"
     CDS             complement(12003..12926)
                     /gene="prmA"
                     /locus_tag="PK522_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNC40947.1"
                     /translation="MYWNSLTLDVDAAQVELVSEKCFVLGALSIDIRDAAADTADEKP
                     LFGEPDVIHDEIWDHTFVSALFEHHVDIAIILQEIKQTLNLPYPPKYALAQVHEQDWV
                     KLTQSQFDPIKISQRLWIVPTWHTPVDTNAINLILDPGRAFGTGSHPTTQLCLSWLDQ
                     QFQPGDAVLDYGCGSGILAIAALKLGASRVIGVDIDANAIQASQENAMINQCNPQSIL
                     FSTSLNLPIANFFEKRYVDKVVANILANPLILLAPLLANAVRKKGQIALSGILKEQAD
                     EVCQCYRQWFDIDIVDQKDDWVLLVGTKYLD"
     gene            complement(12945..14312)
                     /gene="accC"
                     /locus_tag="PK522_04105"
     CDS             complement(12945..14312)
                     /gene="accC"
                     /locus_tag="PK522_04105"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNC40948.1"
                     /translation="MFEKILIANRGEIALRIQRACRAMGIKTVAVHSEADADAKYVKL
                     SDESVCIGPAPSAQSYLNIPAIISAAEVTDAEAIHPGYGFLSENADFAQRVEESGFAF
                     IGPRPETIRLMGDKVSAKNAMRQAGVPCVPGSDGALPESLDEIKKIAKSIGYPIIIKA
                     AGGGGGRGMRVVHTEAALSNALITTRNEAQAAFSNPTIYAEKYLENPRHIEFQVLVDS
                     YGNAVHLGERDCSMQRRHQKILEEAPAVKISSRLRDKIGERCTEACRRINYRGVGTFE
                     FLFENNEFYFIEMNTRLQVEHPVTETVTGIDLVRAQIKIAAGEKLELKQKDIIFQGHA
                     IECRINAEDPYKLTPSAGRILQYHSPGGPGIRVDTHVYHNYMVPPYYDSMIGKIIAYG
                     DDREQAIARMRIALSEMVIHGIKTNIPLHLDLLSDSHFLDGGTSIHYLEHKLALHNKS
                     DKGDR"
     gene            complement(14374..14853)
                     /gene="accB"
                     /locus_tag="PK522_04110"
     CDS             complement(14374..14853)
                     /gene="accB"
                     /locus_tag="PK522_04110"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNC40949.1"
                     /translation="MDLRKLKKLFELVDEHNITELEITEGEEKVRISKNSNQIIQGYT
                     IPPQQSHFSYSGVAEASQKQTVNESVDSDDKPKTELPDGHIVTSPMVGTFYRSSAPGA
                     KPFVETGHSVKVGDTLCIIEAMKLLNEIDADKDGIIKSILVENGQPVEYGQPLFVIG"
     gene            complement(15001..15450)
                     /gene="aroQ"
                     /locus_tag="PK522_04115"
     CDS             complement(15001..15450)
                     /gene="aroQ"
                     /locus_tag="PK522_04115"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNC40950.1"
                     /translation="MQKNITAKNILVIHGPNLNLLGIREPDIYGTVTLEVINHKLLEM
                     AQAAGIQLNIFQNNSEGLLIDRIHSTFQDGTEAIIINPAGLTHTSVALRDALAATNLP
                     FVEVHLSNIYTRESFRHKSYFSDLAVGIISGLGHKGYEFALQFFIKS"
     gene            complement(15668..16696)
                     /locus_tag="PK522_04120"
     CDS             complement(15668..16696)
                     /locus_tag="PK522_04120"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNC40951.1"
                     /translation="MINPANPTDRNTTHVEYLDGWRGLAIFTLLIGHFFPVPGINFGT
                     VGVNLFFVLSGLLMGGLLFEKKEPISRFYRRRVARIVPAHATFIFLITISYFALDLPF
                     SFVEFASALLFINNYIPPTDGPGNALMPFGHIWSLSVEEHSYVILSIVAIVSRRRLFS
                     SSSAIGLLVIASASCAVYYQWLNPPKLAFSEWLHTEVAAYGLLCTAFWVSSGRPMPSR
                     FTSHSLAPILLITGIVLHWWSTPLVIQQLIGVLCFVGAICVLSTTKGWFASALSWSPL
                     RKLGLWSYSLYLWQQPFYLWSHSEQGPSAWIALCAALFCGLISYYFVEQPVRIYLNTN
                     WKTRRRDS"
BASE COUNT         4986 a         3461 c         3722 g         4837 t
ORIGIN      
        1 accataatca taagccgacc cgatatcccg tcccaagagt ctagacaatt ccagcaacgc
       61 ttgacgataa acttgtcgct tgaattctac gacagagtct agctctaccc aatactggtt
      121 ccagcgccat gcgtcaaact cagggtgtgg gctttttctc agtgaaacat cggtatcgcg
      181 tcccaataat cgcaacaaaa accagatttg tttttgtcct ttgtagcttt tgcgacactc
      241 acgtcggatc caacattccg gtacgttata tcgtagccat tcacgcgttc ttccaagaat
      301 ttctacatga ttcggttgta atccaatttc ttccgttagt tctctataca tcgcctgtgt
      361 tggggtttct cctgttttga ttcccccttg aggaaattgc caagaatttt gtttgactcg
      421 ctttccccaa aaaacttcat tttttgaatt tagcagaatg ataccaacat taggacgata
      481 tccgttccga tcaatcattt gattcatccg tcattcaatt cgttaattag cggacatttt
      541 ttcatacttt ggataagatg aaaagccacc ataaaacaaa aagctgggac tttttccttt
      601 tgctctaacc tttgcgcaat ttttatcaag ctaagatgag ctgctggcaa gtctataacg
      661 tattgatgat tggttttcaa ggtagaatca cgttttaaat aaaataatca taagtggaat
      721 caattttatg cgtgtttcta atttctttct ttcgacactt aaagaagcgc cagcagaagc
      781 agaattaatt agccataaac tcatggttag ggctggtttg atcaaacgtt tgggaagtgg
      841 gctttatagt tggatgccat tggggctgag agttttaagg aaagttgaac agatagtacg
      901 agaagagatg aatcgaagtg gggcacaaga gctattaatg ccggctgtac aacctgcaga
      961 gctgtggcaa gaaacagcac gatgggaaca atttggacca cagatgctga aaatcaaaga
     1021 taggcatgag aatgactatt gttttggacc gacgcatgag gaagtgatta ccgatattgt
     1081 tcgtcgtgaa attaaaagct accgtcaatt acccatcaac ttttaccaaa ttcaaaccaa
     1141 atttcgtgat gagattcgtc cacgatttgg ggtgatgcgt gcgcgtgaat ttatcatgaa
     1201 ggatgcctat tcgtttcatg tagatcaagc gagcttagaa aaaacgtacc aaatcatgta
     1261 tgaaacctat tcgaggatat tcactcgaat cgggttagat tttcgagcag tggctgccga
     1321 tacgggagca atggggggca gcagttcaca cgaatttcat gtgcttgcag actcggggga
     1381 agatgcgatt gcattttgtc cccattcgga ttatgcagcc aatgtggagt tagccacatc
     1441 cttgcctgga aattcaatgc catctgaagc gcctactgga gcaaaaaata aaatttccac
     1501 acctggtatc aaaacttgcc aggaggttgc ggaatttcta gggattccga ttcagaaaac
     1561 ggtaaaagca cttgcaatta ctgcggatga gcaatttttt ttattgttgc tgcgagggga
     1621 ccatcagctc aatgaaacaa aagtcagaaa aattccatta ctgactaatt ttgaattcgc
     1681 aagtgaagaa aggatcatta cagaaacggg ctgtaagcct ggttatatcg gaccgattgg
     1741 tatggaaaag tacctcatcg ctgatcgatc ggtattggag atgagtaatt ttgtgtgtgg
     1801 cgcaaatgaa gaaaattttc attttaccca agtcaatttc ggccaggatt tgaaagtgcc
     1861 tgatcgctgt tttgatcttc gcaatgtcgt agcaggagat ctttctcccg atggcaaagg
     1921 gcagttagag atatgccgcg gtatcgaagt cggccatatt ttccagttgc ggactaaata
     1981 ttcggaaaag atgaaagcaa cttttctgga tgatgcaggg caaatcaagg taatggaaat
     2041 gggatgttac ggtattggcg tttcgcgcgt tgtggcagct gcgattgaac aaaaccatga
     2101 tgctgcaggg attatatttc ctaccgcaat tgcacctttt gaagttgcta tcgtgccgat
     2161 tggataccat aaaaatcaat cagtacaaca ggccgctgat caactgtatc agacgttatc
     2221 tcaagcgggt gtggaagtgt ttttggatga tcgggatgaa cgtcctggcg tcatgtttgc
     2281 cgacatggag ttaattggta tcccgcatcg tatcgtgatt ggagaacgtg gcctcaaaga
     2341 aggtgcagca gagtatcgag ggcgtcgtga tcaagcggct caaatgattc caattgctga
     2401 agccgatcat tttcttcgcg caaaggtatg cggaacctag cattaatcat tttattattg
     2461 aagccttgct ggatttttgc aggcgctcaa ctttatgagc cattatcagc gagtactcga
     2521 accgtgcttc agaaagcaat tagcgatcag ccgattgctc aattcgtgac tttgcctgat
     2581 gcggagagta gaagatggct tgcagctatg tcagcgcgta tggaaaaata tatgcctgat
     2641 cagaatgagc gtgaaggttt cttaacgaca gttcattacg aggcgatgcg agccggtctt
     2701 gattctcagc ttgtgctgag tatcattcat gtcgagagta gatttcgcaa atacgcaatt
     2761 tcatcggctg gtgcaagagg gtatatgcaa ataatgccat tttggattga taccattggg
     2821 agcaaagaac ataatttgtt tcatttgcgc gttaatttgc gctatgggtg taccattttc
     2881 cggcattatc ttgatttaga aaaaggtgat tatttcagag cactaggtcg atataatggc
     2941 agcctgggtt cagcaaccta tccggatttg gtatttagtg cttggcaaaa aaattggttt
     3001 ttctcaaagg attaatcctg tatttgctaa aaagttactg atgggtggtt acattttctc
     3061 tctactaaac acgacaacgt gatccgctgc caactggatc ccgatacttt caccaatcga
     3121 atgattgtgg tgactcggga ttaacgacag gagagtttgt ccgctagata gcttgagtgt
     3181 gtagaggata tttgcaccgc ggaatgcctt attgacgacg gttgcttgta aagcactagc
     3241 ttcgttgtaa acaatatcat cgggtcggag taaaacatcg actgcttcgt tggtggtatg
     3301 ttcagattgc tcaaagtaat ctgaataatc gggattttcc acgataccta acgctatttg
     3361 aattttttca ggtgaaatca aatggcctgg aacaaaagta ccttgtccga taaattgtgc
     3421 tacaaagcga tttgtaggat gatgataaag attgtaagcc gtatcccact gcaaaatttc
     3481 gccttgatgc atgacgccga taacatccgc aaccgagaaa gcttcagttt ggtcgtgggt
     3541 gaccaatatg gctgtcgtat gttggtcttt taaaatatct ctcacttcta aactcaagcg
     3601 ttcacgaaga ctaatatcca aattcgagaa aggttcgtca agcagtagca ggtcaggacg
     3661 aggggcaagt gcgcgtgcta atgcaacgcg ttgttgttgt cctccggaga gttcgtgagg
     3721 atagcggtcg gaagcctcag tgagtccaac ggtagttatc aattcattga ttcgatgagt
     3781 acgttcagct ttcgtgtatt gttgtaaacc aaagccaata ttggctgaaa cgctaaggtg
     3841 tggaaatagt gcatagtctt ggaaaaccat cccaattctt cgttgctctg gtggtagatt
     3901 gaaatcagca ttgcagacga ttttcccatt gatcgtgatt tcaccagatg agatggactc
     3961 aaacccagcg atacatcgca ataccgtcgt tttcccacaa ccgcttggtc ctaataaaca
     4021 accgatttca ccttttttca attcgagcga gaggcgtttg atgatttctt gctgatggta
     4081 ggactgatgt atttgggtta attttaataa tgattccacg atgtttactg ttcagtttgt
     4141 cgcatcaata agataatggg aattaaaccg gctaatacga gtgcaatggc aggcaatgca
     4201 gcctgattcc atgcgccttc tgaggttaaa gtgaaaattc ggatggctag cgtgtcccag
     4261 ccaaaaggcc gagtcatcag cgtaatgggc atttctttca tcacatcgac aaaaacaagg
     4321 gtggcagccg taaaaatacc cgagctgaga atcggcaagt ggatacgaga aagtaatgac
     4381 caaccggtta gtccgaatcc gcgcgcagct tcatcgatac tgcgagtaac acgctgcatc
     4441 gcgctatcaa ttggataatg acctaccgcc aagaaacgaa ccaagtaagc gataagcatg
     4501 gctataatgg ttccttgaat cagaaaacca gcttcgatct caaataatat cagaatgatt
     4561 tgattgagtt gtgtatcgat ccaagctagc gatataaaaa taccgactgc gagtactgcg
     4621 ccaggtaacg catagccaag cgtagcaata cgaaccgcga tgtgcgtggc tttatctgaa
     4681 taaaggcgag tagcataaac cagcaaaata gcgaccgagc cgatgatcaa tgtagcgatt
     4741 gtggacaacg ttaatgagtg gatgagaaat tcaatatacc gtgagtcgat atttttatta
     4801 atttcatcga ttgcccaaat aagtagttga gagcaaggca aaataaaagc gataaaaagg
     4861 atcgagaaag cgtaactaca agctgcaagg cgcttccaac ctaagagtgg gatacgaaag
     4921 gtcgttctgc ttgattttga ttcggcaaag cgcatccggg cacgaagttg ctgctcaatg
     4981 ataatgatga taaagacaag aatgatcaat aatgatgcaa gctgggaagc tgcagacagt
     5041 gagaacaaac taaaccatgc tttatagatg gcggttgtga aggtatcgta attgaatacc
     5101 gagaccgttc caaaatcggc aagtgtttcc atcaaagtta acatggcgcc ccctataatc
     5161 caaggtctcg ccatcggaag tgcaacttta aagaatcctt gtttgcgact caagccgagc
     5221 gattgagctg cttcaatcag tcgttttccc tgcgttataa atgcattacg cgctaaaagg
     5281 taaatatagg gataaaacgc aaggaccatt acgagaatag ccccacttct accgtaaaaa
     5341 tcaggaatcc aattagattc cgcaccagtc caacttctta aataggtttg aatcggccct
     5401 gtgtagtcga atagacctag taccacaaat gcagtgacat aagcaggaat cgccatcggt
     5461 agcaataacg cccaagaaaa gaattttctt ccaggaaaat cataaatagc agttaaccaa
     5521 gctaggctag tgcccaggat aatcgctccc aagacgacgc ctaaacataa ccagaatgta
     5581 ttcaacagga ctgagggtag ggttgtttca gcaagatgat gccatatatc atctgctggt
     5641 gtaaaaatcg aggaaataac aacaatgatc ggtattaaaa cgatactggc aacgcagaaa
     5701 ggagctaaac gccagaaatt gatgttattt tttagttgag gagagggatg tcgatggctg
     5761 gctgaaagtt ttgctcccaa attatccggc tgttcgtcat tcacatggat tgacccaatt
     5821 agcggtagcc ggcacgatcc attaatttaa ccgcagtggc ttgtaattcc ccagctttaa
     5881 tcatactcat gggacttttc ttaaaatcac cccaactcgt aacaatcggg cttggcttaa
     5941 tttggggatt ggcaggaaat tcaaagttaa catccgcaaa taaattttgg gctttctcag
     6001 aagacaggaa ttcaagtaac ttgattgctg cagcttcatg tttactatgt tgtgttacgc
     6061 cagcacctga aatgttgatg tgggtaccat tgttatcttg gttgggccaa taaataacca
     6121 gcggtaaatt tgggttcgtt tccatcaatc gaccataata atagctattg actaatgtga
     6181 catcacagcg gcctgctgca acatattcca ttgctttggt atcgtctgat aatggttcgg
     6241 tggcaagatt tgctacccaa cccttaacga tttgctcggt ctttgcttca ccatgctcgg
     6301 caatcatcat tgcaacgagc gattggttat acactttttt ggaagagcgc aaacacaggc
     6361 gccccttcca tttagggttt gctaagtctt cataggttga aagttcagaa ggatcaactt
     6421 tttccttgtt atagacgaga gtgcgtgccc gtatcgataa gccataccat tggccttgtg
     6481 gatctctcag ttgtactggg atattggctt ctaaaatcgg cgaagaaatt gattttagta
     6541 atccagctct agcagcttcc catagattcc ctgcatctga ggtgatcaat atgtcagcgg
     6601 gggttctttt gccttctgct tggagtcttg ctaataaagc gccttcttta tcggtagtaa
     6661 atttgacttt gatgccagtt tcagttgtga atgcatcaaa catgggtttg attaactgct
     6721 ccattcgggc tgaataaacg acgacttctt gcgcatggag tgggctagcc ataaagaaaa
     6781 agctaatgca gagcagaaga atcggtagca gttgtttagc agaaagtaat gaggtcattc
     6841 ttatacgata attaacaaga aaaataataa ggttagggat aaagcgtcat tcagaatggg
     6901 gtaaattata ctatgattaa gaataatgat cagttagcga aagcggttat ggtcacgggt
     6961 tgctattatg ctaagtgtca tttgtcaagc aagcgcctgt aagggcgcgg tagattgtag
     7021 tccaaatgct tcagcaaccg ctgtgtgagt gatcaatccc tgataagtat tgagaccttt
     7081 ctgtaatgcg ttattgtttc ttactgcatc taatccgcta tcagccagta gcttaatata
     7141 agtggcagtc tgactattta atgcgagcgt actggtacgg ggtacagcgc ctggcatatt
     7201 ggtaacacaa taatgcagaa taccatcgac ttcataaacg gggttactat gtgtggttgg
     7261 atgggtcgtt tcgacgcacc ccccctgatc tacggcaaca tccacgataa cagcgccttt
     7321 gcgcattttt gttagcattt ctcgagtgat gagccaaggc gcccgcccac ctggaatcaa
     7381 tacggcaccg ataacgagat cggcttgata aacgagttca ttgaggttga atgaatcatt
     7441 catgcgggta aagagttgtc cacgaaacaa atcatcgagg tatttcaaac gactatgatt
     7501 gatatctaag atcgtaacat gcgcacccat tcccacagtc actctagcag cattaacacc
     7561 gacaatgccc gcaccaagga tgacgacatt agccggagca acgccaggga caccccccat
     7621 caatattcct cttccaccca acgttttttg taaatagagc gctccaattt gagcagccat
     7681 tcttccagca acttcactca tcggcgtgag taggggtaat tcacctgatg ctaattgtac
     7741 cgtttcatag gctataccgg ttgttcctgt cgcaagcagt tgttcagtca gcggtttatt
     7801 tgaggccaaa tgcaggtagg taaatagaat caaatcttgc cttagataag catattcgga
     7861 ttcagtgggt tcctttactt tgataactaa ctgggcagcc caagcatctc tagggtacgt
     7921 tacgatttca gcgcctgcca atcgatagtc atcatcagtg aaagcactac ctaatcctgc
     7981 accactttct atttttatat ggtgtccttg tgtggtgagt gcgtgaacag tcccaggtgt
     8041 cagcccaact cgaaactcat ggtcttttgt ttctttaggt actccaataa tcatagggtc
     8101 tcttacagat aaattgtttg cattgagaat tgtgtattcg tctgctagtt tatcattttg
     8161 cagtttgttt ggtcgggtta tggttgagct aaagcccgag tgaagtcatt aaactacatt
     8221 tattttttta aatatttttt atgtcaggaa tttttacact cgaattgccc tcgaacaatt
     8281 ttccagtgag tgtgatattt tttcgtgctt atgatatcta acaaaatctc actaagaaaa
     8341 agcgtcggat tattttacag ttatcattta attctgatcg aaaggattgg agttgctttt
     8401 attatctata aaaatcaatg aataacaggt gtaaaaatta attggcatgt gaattgctta
     8461 ttaaaaatat ataataataa cgatgaaagt tctgcgaaat gctaactatg ttatgtagtg
     8521 ataatagctt taacagaaat gaggtattag tataaaagct tgtcatgatc taaattatcg
     8581 gatctgacaa gcttatttta tttttgatga gtcaaatggc gatcgtcaaa ttccgtcacc
     8641 gcaccggtaa ataactggtt tgattcttat tcttcattct ttcctgcttc acatcgcgtt
     8701 gcctttataa acttcgagtg ggtttatttt ggaaaacctc gttcttcttt agcaaagcta
     8761 attcgatttt acaaacacgg gcgttctccg tcttgttatg atatgttaat tcgttgtgaa
     8821 atctaatctg atttaaggtg cgagcggaaa ttatgctgaa atatgactat tcgttatcga
     8881 agaatgagca aatcgcatta tttttcttta ttatcgggct cattacggta tttggcctgc
     8941 tgcttttttc aaataaatcg gaagaacgca ttgctgaaag ctcaactcat cttcagcaag
     9001 atctcacaaa gcagttttat caacaaaaat tacagcagcg tgaagcagaa attctcgagg
     9061 tgcttaagca cactgaccct aaacataggc gcattctcga agttgaattg caggatatcc
     9121 agctgctttt acaggatatt gataacagtt tcttactgct aaaaacaagt atcaatgacc
     9181 gcctaaaaca attagaagta cttcatcaag cttatcctat tcaaatgcta gaacaggcta
     9241 ttatggcttt acgtcaagcg aatgatcagc aggcaattca actattccgt caaatcgaaa
     9301 aaaataacga gcacaatcca ccccttgcgg ctgaagcagc ttaccaattg agcaaattag
     9361 ctgaagattc agttcgctat acggcaggac ttgaacatgc agaaaaggcg atccgcttga
     9421 ttcctaataa tgcgttatac ctcagcgcgg cgggattttt tagcgataaa ttagcgcact
     9481 acaagaaagc cattagttac catgagcaag cactcgcaat tctgcgcacg ctacactctg
     9541 aaaatcatcc tgagattgct gcagtaaaga atgatctggg tatggcatgg gatgcactcg
     9601 gtgaatacgc caaagcgatc aagttttttg agcaagcact ggaaagtttg attcaattgc
     9661 atggagaaaa tcaccctgat gtcgctgcca ttcagaataa cattggaatg gcttgggact
     9721 cattaggtga ctatcctcgc gcgattcgat attatgaaca atcactggaa gctgatctca
     9781 gtcattatgg agaagatcat ccgagtattg ccaaacgccg taatcaaatc gaccatgctt
     9841 gggaatctat tgaagcctac catgatgcca ttcagtattt cgaagcggct cttgatagcg
     9901 accttaaaac ctataaaacc aatcacccta gtattgccag agatcgtaat gatttaggaa
     9961 ttgcctggga atcgctcggc gagtatcaca aagccattga gtattttgag caagcactcg
    10021 aaaccagtgt tgcgatttat gggaagcatc atccattgat cgttacttac ctgaataatt
    10081 tgggtaacgc atgggactca ctaggcaaat atcgaaaagc catcgaatat ttcgagcaag
    10141 cgcttgagac tgggcttgca ttgcatgatg aaaatcatcc ttccattgcg gtgacccgta
    10201 ataatctcgg caatgcgtgg gaatcgcttg gggaattgca caaagcgatc agttattatg
    10261 agcaagcatt ggcgattgat ttaaaggcat atggtcagaa tcaccccaac attgcgcgcg
    10321 acaaaaataa tttggggaac gcttgggaat ccttgggtga gcatcaaaag gccatctact
    10381 attttgatca ggcgctttcc attgtgttgc aatcgcatgg tgagaatcat ccacaagtta
    10441 ctacatatcg caacaatctt ggctgggctt ggtattcatc gggacattac caaaatgcca
    10501 tcgattatta caaacaggcg ctggttacag ctaaaaaatt acttggggaa gatcacccga
    10561 acacaaaagt gattcaaagc aatttaagaa gcgcagagag taagctaatc gcaacgccct
    10621 aatttcactg ctatcagtta aaccgtgtat gtgaaatatg gaactcatca gattaagcag
    10681 cgagcagatt ctaacgtaaa aaatctaggc taaattcata ctgaattata actttcaaat
    10741 cggggagtgc catggccatt gaaatcagtt gggaagcagc tagcaacaaa acggcttgcc
    10801 aaatacaaga aaatggaaaa acttggatct cggatatcgg gctagagtct ggtgggtctg
    10861 gtcaatatcc tgatccgcat cagatgctcg actctgcgct tggcgcttgc actgttctta
    10921 ccattcaaat ctatgcgaaa cgtaaggcga taccattaga gcgtgttctc gtatcgatta
    10981 cgcacactga agaagcaggt ttataccaat tacagcgcaa tattcaatta aaagggcagc
    11041 ttaccgaggc gcaacgcgat gatttgttac gagttgctaa taaatgtcct atccataaag
    11101 tgctttcagg acagtttaca atcatcacgc aacttgaaaa ttgatgcaat cgtgtttgtg
    11161 catcatcaac ttgaaccacc tatatcgttt aaagatagat taactccaaa cgatacccca
    11221 tggctttcaa ctcactatta tcaaaataga ttttgatttc tatttcgcta tccggagcga
    11281 agaattgtgg cagtttatta tcgtttagat agtcacttgc caacaaaatt cggcttgcaa
    11341 tgggttgatc atcaagatcg gttagtgtta atcgtaattc aggccatgct tgctgaaagg
    11401 gcgcgtgatt acggatcgtt gcaagtaatg tcacaatttc agcttgatgg ttaggattga
    11461 cacgtaattc agaggattca agactcaata aggcgctgta ttttggtaag tgcacttcac
    11521 attgcaatag atcgcaactt ttttctagaa tcgctctgga acttggaatc gcgattgcga
    11581 gttctgtacg gtaaaagtaa atgagttgta cgcacaacag gataattaaa gctaagcttg
    11641 cagcaacttg tttccaaatc aagttggggg tgtgtacttc agctacccca ttttgatcag
    11701 cttgatcggc tatagatgcg ctcgttaact ccgcttgctc gctggattgc aggcctgatg
    11761 gcgcagggtg tttattaata gcgggaagtt caacttcttc atgaacggga agcgcgagtt
    11821 ggctgtcctc cattctgata agcgattctg gaattgaaat cagcgcccta aaggcattaa
    11881 aagcttgttc acattgtcca caccttactt ttccatcgtg caaatgtaat tctggaaaag
    11941 taacgcgata aactgattgg caaacagggc acttcgttac gagaaccata gcctagcaac
    12001 ttttaatcaa ggtatttcgt tcctactagc agtacccagt catctttttg atcgacaata
    12061 tcgatatcaa accattgccg atagcattgg caaacttcgt cagcttgttc tttcaatatc
    12121 ccagaaaggg cgatctgacc cttttttcga acggcattgg ctaatagcgg cgctaatagg
    12181 atgagtggat tggctaagat attggcgacg actttgtcga catatcgctt ctcaaaaaaa
    12241 ttagcaattg gcagattgag cgatgtcgag aataaaatag actgcggatt gcattgatta
    12301 atcatcgcat tttcttgact cgcttgaatt gcattcgcat cgatatcaac tccaatgacc
    12361 cggcttgcac ccaattttaa tgcagcaatt gctaatatgc ctgagccaca tccataatca
    12421 agcacggcat caccgggttg aaattgttgg tctaaccagg aaaggcataa ttgcgtcgtt
    12481 gggtgactac cggtaccaaa cgcacgacca ggatcgagaa tcaagttaat cgcattcgta
    12541 tcgactgggg tgtgccaagt tggaacaatc cacaatcgtt gagagatttt aatggggtcg
    12601 aactgtgatt gtgttaattt tacccaatcc tgctcatgta cttgagctaa agcatatttg
    12661 ggtggataag gaagatttaa tgtttgtttg atctcttgca aaatgattgc aatatcgaca
    12721 tggtgttcaa ataaagcgga aacaaaagta tggtcccaga tttcatcatg gataacatcg
    12781 ggttcgccaa acaaaggttt ttcgtcggca gtatcggctg ctgcatcacg gatatcaatt
    12841 gataatgcgc ccaacacaaa gcatttttca ctcactaatt ctacttgagc tgcatcaaca
    12901 tcgagtgtta gcgagttcca atacatgatt gagcaaagga aaggttaacg atcaccttta
    12961 tccgatttat tatgcagagc gagcttgtgt tctagataat gaatcgaagt acccccatct
    13021 agaaaatgac tgtcggataa taaatcgaga tgaagcggta tattagtttt tataccatga
    13081 atgaccattt ctgagagcgc gatacgcatt cgtgcgatag cttgttcacg atcatcccca
    13141 taggcaatga ttttaccaat catcgaatcg tagtaaggtg gaaccatata attgtgatac
    13201 acatgtgtat caacacgaat accaggccct cccggcgaat ggtattgcaa aatacgtcct
    13261 gctgaaggcg ttagtttgta ggggtcttca gcattgatac gacattcaat cgcatggcct
    13321 tggaaaataa tatctttctg ctttaactcc aatttttcgc ctgctgcaat tttgatctgc
    13381 gcacgaacta aatcgatccc tgtcactgtt tcagtcaccg gatgttcaac ttgcaaacga
    13441 gtattcatct caatgaaata aaattcattg ttctcaaaca aaaactcaaa tgtaccaaca
    13501 ccgcgataat taatgcgacg acaggcttca gtacagcgtt ctccaatttt atcgcgtaat
    13561 ctcgaagaaa tttttacagc cggcgcttct tcaagaattt tttgatgacg gcgctgcata
    13621 gagcaatctc tttcgcctaa gtggacagcg ttaccatacg aatcgaccaa cacctgaaat
    13681 tcaatatgac gcggattctc aaggtacttc tctgcataga tcgttggatt actaaaagca
    13741 gcctgcgctt cattgcgtgt ggtaatcagc gcatttgata aggctgcctc ggtatgaacc
    13801 acgcgcatgc ctcgcccacc accgccaccg gctgctttga taatgatagg atagccaatt
    13861 gattttgcta tttttttgat ttcatcgagc gattcaggta aagcgccatc cgatccggga
    13921 acgcaaggta cgcctgcttg tcgcatggca ttttttgcag agactttatc acccatgagt
    13981 cgaatggttt ctgggcgagg cccaatgaat gcaaacccac tttcttcgac acgttgtgca
    14041 aagtctgcat tttctgagag aaaaccataa cctggatgaa tggcttcggc atcggttacc
    14101 tctgcagcgc tgatgatggc agggatattg aggtaacttt gcgctgatgg cgctgggccg
    14161 atgcataccg actcatctga cagtttgaca tatttcgcat ctgcgtctgc ttcagaatga
    14221 actgctacgg ttttaatacc catagcccgg catgcacgtt gaatacgtag cgcaatctca
    14281 ccccgattcg ctatgagaat tttttcaaac atgttgactc atataaaaaa ataaaatctg
    14341 aagggagaaa ggataattta tatcagtact tgcttatcca atgacgaata acggctggcc
    14401 gtattcaacc ggttggccat tctcaactaa tatcgatttg atgatgccat ctttatccgc
    14461 atcgatctca ttcaatagct tcattgcctc aataatgcag agtgtatcgc ctactttgac
    14521 actatgaccg gtttccacga aaggttttgc acctggtgct gaggaacgat agaacgtacc
    14581 gaccataggg gaggtcacaa tatgaccatc cggtaattct gtttttggtt tgtcatcgct
    14641 atcaacagat tcattaacag tttgtttctg agaggcttct gccacaccgc tataagaaaa
    14701 atgagattgc tggggtggta tagtatagcc ttggataatt tggttcgaat ttttgctgat
    14761 acgaactttt tcttcacctt ctgttatctc aagttcagta atattatgtt catcaactaa
    14821 ttcaaatagc ttcttcagtt ttcttaaatc cataaaactc ctcctaaagt aaacgccttg
    14881 tcgcgtattt tttcgctatt ttcagttttt ttcatgacaa ttaaagaagt ttaaatttga
    14941 cctcaagcct aaaaacattt cagtgatatc tatcctatct aaacgtgtat taagaaatag
    15001 ttatgattta ataaaaaatt gcagtgcaaa ctcataacct ttatgaccta agccactaat
    15061 aatcccaact gcaaggtctg agaaataaga tttgtggcga aacgattcgc gggtatatat
    15121 attagacagg tgtacttcaa caaagggtaa gttggttgcg gcaagtgcat cccgtaatgc
    15181 gacactcgta tgtgtcaacc cagccggatt aatgataata gcctctgttc catcctggaa
    15241 cgtagaatgg atacgatcaa ttaataggcc ttcgctatta ttttggaaga tattaagttg
    15301 tataccggca gcttgagcca tttcaagtaa cttatgatta attacttcta atgttacagt
    15361 gccatagata tcaggttctc ttattcccaa taaatttaga tttggtccat ggataaccag
    15421 gatattttta gcagtgatat ttttttgcat tgttacgaaa ttatcgcttt tttaagtaaa
    15481 aatgtacaga tttttaaaga caatcagcgc atttatgata agtttcacga tagaaaacta
    15541 tcaagtggtt attcctactg agagaacgtt ttaggataac tgcgcaatgc aaaaatgttt
    15601 tgtgagtcgc tctgggagtt ggagttatcc cctttgagct agaatacacc cgcccaaagc
    15661 agctaagcta gctgtcgcgt cttcttgttt tccaattggt attcaagtag attcttactg
    15721 gctgctctac gaaatagtat gagatcaatc cacaaaacag agctgcgcac aaagctatcc
    15781 aggctgatgg accttgttcg ctgtgactcc aaagataaaa aggctgctgc cataaataaa
    15841 gtgagtacga ccatagacca agtttgcgga gaggagacca tgaaagagca cttgcaaacc
    15901 agcctttggt agtgctaagt acgcagatgg cgccaacaaa gcataaaaca ccaatcaact
    15961 gctgtattac gagtggagta gaccaccaat gaagtacaat acccgttatt aacagtattg
    16021 gagcgagaga gtgtgaagta aatctagacg gcattggcct acctgaagaa acccaaaaag
    16081 cggtacagag taacccgtat gcagcgactt cagtatgaag ccactcagag aacgctagct
    16141 ttgggggatt tagccattga tagtaaaccg cgcaagaagc agaggcgatg acgagtaaac
    16201 cgatagcact agatgaagag aagagcctcc tccgagaaac aatggcaact attgaaagaa
    16261 tgacatagct gtgctcctct accgatagag accagatatg gccaaaaggc attagcgcat
    16321 ttccgggacc gtcggtaggg gggatataat tgtttatgaa taggagagcg gaagcaaact
    16381 ccacgaaact aaatggtaaa tctaatgcaa agtatgaaat agttatgaga aatatgaatg
    16441 tagcgtgggc aggaacgatt ctcgccactc ttcttctata aaatcttgaa ataggttctt
    16501 tcttttcaaa tagaagtcct cccattaaca aaccggaaag cacaaagaag agatttacgc
    16561 ctactgtccc gaagttgatg ccaggtacag ggaaaaagtg gccaatgagt agtgtgaata
    16621 tcgcaagacc gcgccaccca tcgaggtatt cgacatgggt ggtatttcgg tcagtcgggt
    16681 tagctggatt gatcatagaa tcagcgctgg cgtcaaaata tcgagaagca agggagtagg
    16741 atgttgtgtt gcggacagtc agtcgacgac taactacaat caaacatcca aaactaggca
    16801 gtataatata tcttaaatga aatccaatct ggaattgttc atgatatctc cccttatgtc
    16861 ttataacaac caactgattt aattgtcatt ttaacaaact tgtctagatt cacaaatatt
    16921 ttaacggact catgtcgaga actgaatcgc cccgggtttt ccggagacct tttagtttga
    16981 gtcaagccac attagctgac tcctct
//
LOCUS       DAOQLJ010000035        16811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1228_length_16811_cov_6.791776, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000035 DAOQLJ010000000
VERSION     DAOQLJ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 16811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16811
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1228_length_16811_cov_6.791776"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="PK522_04125"
     CDS             <1..1452
                     /locus_tag="PK522_04125"
                     /inference="COORDINATES: protein motif:HMM:NF016903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HNC40952.1"
                     /translation="QQHSAEDDIAFIADSARYMRDPRYIRIEGKPLLILYRPGLLPDA
                     RATAMRWRKWCRENSIGEIFLALTHAFEHTKPNEIGFDAAIEYAPNTYPVKPITQQII
                     ASGKMINPLYQGNIYDYNEAASIGQNQIMPSYKKFRGLFPGWDNEARIPGRGTTYIDS
                     SPLRFHQWLATLISLSQKQFKPSEQFIFINAWNEWAEGAHLEPDRKFGFSFLESCRIA
                     QQLEILSQQKNNLISQENYPKVAIVIHAYYPDIFDEILENLSSTDKYKIKLFITTPSY
                     QVSLIENRLISHGMEYQILGVNNQGRDIFPLIKILKEIYQQHFSFIVKVHTKKSKHRT
                     DGTIWRKDLFFKLLTKSMLEKNIQYLVDHPEVGILAPEGHLVPMNHYIAANEKAIIEL
                     SARLGVEMETVMKLHFVAGTMFTARIDALLPLLTLSFEDTDFGVEKGQLDGTLAHALE
                     RLISIGNHRIGYQIRTLSGQTTSHYAHADVTSR"
     gene            complement(1456..2412)
                     /locus_tag="PK522_04130"
     CDS             complement(1456..2412)
                     /locus_tag="PK522_04130"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC40953.1"
                     /translation="MNILVLGARGFIGKHLCSALVAANHRVRGFDLQPVNGNWPDIPG
                     VEWTAGNLDHVSDVMTALDKIDLVFHLISTTIPQTSNENPLLDLQQNVGATLKLLELV
                     IKQPRKPRFIFLSSGGTIYGIPHIIPVPEEHPTDPLSAYGIAKLAIEKYLALYGYLYK
                     LNYSILRLANPYGEFQSHRSSQGVIAAFVHKALHHKPVEIWGDGSIVRDYLYIGDVIS
                     AMITLIDYDGPEKIFNIGGGKGHSILELLSVIKELFQHDIAYYHLESRACDVPINVLD
                     IKRAASILGWKPTTPLHEGIKQVIDYQRSVDFSLNDNSSKNR"
     gene            complement(2409..3662)
                     /locus_tag="PK522_04135"
     CDS             complement(2409..3662)
                     /locus_tag="PK522_04135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC40954.1"
                     /translation="MKILTISNLYPPNIVGGYEVLCFEVMRGLAEKGHEINVLTSDYG
                     GKQAIYPNQQVQRLLTLLATEGNIYKPLNISAIERNNINQKNLQTLSRTLDEINPDLI
                     FIWNLHFFDVSLLDALSLVKKKQVYLLSDNWLINFYNSDFVANYFSREVYVKRDRWYA
                     CWHRFKSRLFNGQKKRSMLGYAIFASRFMQNLYSRAGFEFANSKIIYHGISSGVEESL
                     PFAKRETFIANDELRLLFAGRVVDIKGVHTAIEAMPHIINALPNLKISLSIVGDMRDQ
                     QYVANLKKIIAELGVEERVCFIAPVEESKLFGLFQDYDIYLFPSLYEPFSLTLIHALR
                     AGIPTIASDVGGNPEIVFHEQTGLLFRPADARHLAAQVVKFVHNKNLRVTVSSGARDH
                     ANQFTFNRMINQIEDYLLYSLESNQ"
     gene            complement(3659..4585)
                     /locus_tag="PK522_04140"
     CDS             complement(3659..4585)
                     /locus_tag="PK522_04140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC40955.1"
                     /translation="MIDMQNRVLELNPTISVIIPLYNHERYIDDAIDSLLQQTIPPFE
                     IIIIDDGSHDNSWQRVQHRAKEEPRIITWSQKNQGAHYTLNNGLNHATGEYLAILNSD
                     DVYHPQRLEFCLQAMQANPVPDVICTALAFINEKGKSCSNTWYEEALAFHRKVSDLSL
                     ALINGNFLMTTSNLFMHRSVWADLGTFANLRYAHDLDFFLRAILFNKRIVILENPLLH
                     YRTHATNTINEGVLKVKLEWAAVVAYYAWKMKRNDWSYWEQLVEITNRHDLTRMLFFF
                     FKYYQQNVTERLAVDDWLADNAFCKFLTDTIR"
     gene            complement(4585..7518)
                     /locus_tag="PK522_04145"
     CDS             complement(4585..7518)
                     /locus_tag="PK522_04145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC40956.1"
                     /translation="MENLLIPYQATTIIPCKKVLVFAPHPDDEVFGCGGAIMRHLAQG
                     VAVEVIIVSDGAYGAKSEESSRQYVLQRQEESNLAAKILGYGLPSFWSYPDRKVGYGE
                     KLIQDILAAINQSQADLIYAPSVFEVHPDHRSIGMAAIEAVRRIGKSLKLALYEVGMP
                     MRPNCLLDISEFVERKMAAMQCFSSQNQKQRYDLDIAALNRYRTYTLPAEIQAAEAYL
                     IISAEELVKDPLKLYQSEYARQRELGVAIDRGDIPLVSVIIRSMDRPTLTRALDSLAL
                     QTYPNIEVVLVNAKGGMHEQSSDWCGNFPLRLINQDGEPLLRSKAANIGLDACQGSYI
                     SFLDDDDAIDPDHIANLIEGIQSVSTCSIAYTGVRGKDEQDENKPFVEFKTAEVNFAK
                     LLLGNVIPIHAILFPATLLKQGAVFDEKLALYEDWDFWLQLSRYTPFNFINRTSATYF
                     TGGGSEVSPLHNDPVRVKQATVNLYQKWLKLLTASEFAAVADLHHRTNNQFQAEMGGL
                     RSEISRLQDLQQQTRFQLEQKQQELDILYASRSWRLTLPLRCCLERVRALKSVFKRCY
                     YTLPGAYRIYRFVREFSQQRRRQSSSQIDQSGGPWPIGPAGQILLIEHSVPRPDQDAG
                     SMMIYNFIKVFIEMGYGVTFYPVDLAYDPVYTPRLIELKVRCLNKPQVNALEHHLAAI
                     DRSYDFVLSCRPVYTEALIPIIKKYCTTAHFIYETHDLHFIRLQRQAEVEHNREMLAH
                     SSFLKAMELNIAAEADCTIVVSHKEKEILLQENPNLYIEVIPVVSEVFGRKSDFTQRN
                     DLVFIGNYEHRPNVDAVLYFVQDIFPHVLKKIPTIKFFIVGSNPPQEITALSSSNIVV
                     LGFVPDITDLMNQIRISINPLRFGAGIKGKLITCMSYGIPCIGTGIAAEGMSLTHEKN
                     ILIADTPSAFSDCIINLYSNPILWEQLSNEGLAFVQQNFSLNVAKEGFTQIFNRLRLK
                     NKTVK"
     gene            complement(7557..9359)
                     /locus_tag="PK522_04150"
     CDS             complement(7557..9359)
                     /locus_tag="PK522_04150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC40957.1"
                     /translation="MNGFSSKQEISDGSITINSPSLFKNFDFPLNVYAHTLFLNEGRV
                     DYLHYGLFESEQINLSQAQQYSTNLILDRLPLPPSRILEVGAGLGTTCHLLTQQGYQV
                     HSITPDRQQIAEIHRRFATGLNVTQQHYEDLEPIAESYDVILFQESAQYIEPLVIFNK
                     AQDLLPEEGHIFIIDEFALQQSETAIGRLHRLKAMLQLSDRLGFELVEHLDLSSKATP
                     TLKYLLHAIGKHRQQLLDDLDLESSRLDQLVQSNQDYLNKYVSGQFGYALLHFRKKRQ
                     LPWRLSRLNTNQMPHMLALFEKIFKHKMTSEMWQWKYGSEQCRAMGVWYDNELIAHYG
                     GMGRSILYFGQPQMAVQIGDVMVDRVNHPGLVKKGPFFQMAATFLEHYIGYGKPYLLG
                     FGFPNDRAMKVAKRHGLYAETGRMVEIAWTPYSRRPLWLSQLKIIDQHNINDNWVVDA
                     INQCWHGMASDLNAAIIGIRDWAYLRDRYLNHPQHHYRIVLVLSRLSRKARGILVLQS
                     NSNGCEIMDMVAPLDQISLLITHTRRIARINGDQRVFCQITHNFSDRFISASGEGMLS
                     ELPIRIPANAWSDGPSPQLLMNHWWLMSGDMDFR"
     gene            complement(9439..10659)
                     /locus_tag="PK522_04155"
     CDS             complement(9439..10659)
                     /locus_tag="PK522_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC40958.1"
                     /translation="MTLKPAVLAHHLSKCYQLYTQPKDRLKQLIWGRRRQYYRELWAL
                     REINLAIMPGEVIGIIGKNGSGKSTLLQLICGTLTPTTGEVEINGRLAALLELGAGFN
                     PEFTGRENIWMNAAIIGLTQQEIADRIEQIIDFSEIRPFVDQPVKTYSSGMYVRLAFS
                     IAISVDPDILIIDEALSVGDGAFARKSFNRIMQLRDSGKTILFCSHSLFQIESLCNRA
                     FWINQGELIAEGDVKQVVTAYQQFLDLGVLSSDNNDIAATIQQNDYLANAKGYARLET
                     VVVEIDGAPCAVAVAESGQSAIQVDVKFSTDPSLPCPTVAVTLHSMDGRVITSAASWE
                     DSIIPRLTPEGIGEIKLLFEQLPLLKGEYLVSVYLLCEQGIHLYDSVYGATKLQVQQQ
                     NRLQGFFMIPHQWK"
     gene            10925..12313
                     /gene="dnaA"
                     /locus_tag="PK522_04160"
     CDS             10925..12313
                     /gene="dnaA"
                     /locus_tag="PK522_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNC40959.1"
                     /translation="MQEFEAFWYTCLKHFEKELNAQQFNTWIKPLQLDISEGCGDQPI
                     LIAPNRFVLQWVKDNFIPHIEAMAQQHFSKRIQFQLVLGEITTKKATPKVNPIIDTSR
                     EDQAVSTVSVADPTPSKKASNKHYLNPNFTFDNFVTGKANQLARAGAIQVAERPGTAY
                     NPLFIYGGVGLGKTHLIQAIGNFILERNHEAKIRYVHAEKYVSDVVSAYQHKSFDKFK
                     KYYHSLDVLLVDDVQFFGGKNRTQEEFFYAFNSLIEASKQVIITCDCYPKEISGLEER
                     LVSRFGWGLTVAIEPPELEMRVAILIKKAALEKISLEENIAFFIAKHIRSNVRELEGA
                     LKRVLAYSRFTGQDLSLELVKEALKDLLAVQNRQISIENIQKTVADYYKIKVAEMYSK
                     KRTRTIARPRQIAMAIAKELTQLSLPDIGEAFGGRDHTTVLHAHRKINELRASDPLIT
                     RDYNALIHILRG"
     gene            12526..13647
                     /gene="dnaN"
                     /locus_tag="PK522_04165"
     CDS             12526..13647
                     /gene="dnaN"
                     /locus_tag="PK522_04165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNC40960.1"
                     /translation="MNLINTKRDTLLKPLQIVSGIVEQKRTLPILSNVLIRKEQEKIF
                     FVTTDLEVEIEVHSIDNENLSKENFSITVSARKFLDILRAFSADTSVVLNKTDNRLQV
                     NISKSRFNLQILPMEDFPRMAIEEKPEVSLTLSQKDLKNCLYHVQYAMAQQDIRYYLN
                     GLLLLTDGQQLKAVATDGHRLGYISMQFEAELIKKETILPRKTVHELVKLLEDSDKTV
                     TIDFYSKKISFSFSGIILISKIIDGKFPDFDRVIPKNNTKQFIIKRLDFLQALQRAAI
                     LSNTSDKFRGVKLIIANNRLSILCKNSEQEEAQEDLDIDYNSQEIIDISFNITYLLDV
                     LNNLDSETIQCSFENANSGTLITIPGDEAFKYVVMPMRL"
     gene            13688..16087
                     /gene="gyrB"
                     /locus_tag="PK522_04170"
     CDS             13688..16087
                     /gene="gyrB"
                     /locus_tag="PK522_04170"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNC40961.1"
                     /translation="MTNKDDREYDSDSIKILKGLDAVRKRPGMYIGDTSDGTGLHHMV
                     FEVVDNAIDEALAGHCDEIIVIIHPDNSVSVHDNGRGIPTGIKQDDELKRSAAEIVMT
                     ELHAGGKFDDNSYKVSGGLHGVGVSVVNALSEWLRLTIRRDKKIHQMEFRKGVPVAPL
                     AVTGESEKHGTEVHFLASKEIFGDVNYHYEIFAKRLRELSFLNNGIKIKLIDQRHDKE
                     ETFAFSGGIRNFVEYINRTKTVLHPAIFYANGSKDNIAVEVAIQWNDSYVEQVLCFTN
                     NIPQKDGGTHLTGLRAAMTRTLNSYIEKNELAKKAKVDTTGDDMREGLTCVLSIKLFE
                     PKFSSQTKEKLVSSEVRPVVEDIVAQKLTEFLLEHPSDTKIICNKIIDAARAREAARK
                     ARELTRRKGVLDNLGLPGKLADCQEKDPANCELYLVEGDSAGGSAKQGRDRKFQAIMP
                     LKGKILNVEKSRFDKLISSQEIISLITALGTGIGKDEYNPDKLRYHRIIIMTDADVDG
                     SHIRTLLLTFFYRQMPELIERGHIYIAQPPLYKIKHGKQERYVKDDHELNQYVLGLAL
                     EGAELQISESNPPIMGEALTSIAREYLLAEAVIDRMSRLIDRSVMYALLKEPDIDLSN
                     QDNAQQSAAKLAAIVQTVQINPEFDDVFEQYRLKLIRTHHGNIQTSYLDQDFVKSNDF
                     EQIKKAAQVLQGLITNGAVVKRGNDEHTITEFKEALDWLLAEAKKGINIQRYKGLGEM
                     NPAQLWETTMDPNNRRLLRAQIEDSILTDEIFTTLMGDVVEPRREFIEKNALQVTNLD
                     V"
     gene            16182..16257
                     /locus_tag="PK522_04175"
     tRNA            16182..16257
                     /locus_tag="PK522_04175"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16215..16217,aa:Lys,seq:ttt)
BASE COUNT         5336 a         3517 c         3159 g         4799 t
ORIGIN      
        1 cagcaacatt ctgccgaaga tgatattgct ttcatcgctg attcagcaag gtatatgcgc
       61 gatcctcgtt acattcgaat cgagggcaag cctttattaa ttctttatcg ccccggactg
      121 cttcctgacg cgagagcaac cgccatgcgt tggcgcaagt ggtgtcgaga aaattcgatt
      181 ggtgaaatct ttctagcact cactcatgca tttgagcaca ccaaaccaaa tgaaatcggc
      241 tttgatgcgg caatagagta tgcacccaat acatatccgg taaaaccgat aacacagcaa
      301 attattgctt ccggaaaaat gattaacccg ctatatcagg gcaatatata cgattacaac
      361 gaggccgcgt cgattggaca aaatcaaatc atgccctcat ataaaaaatt tagaggatta
      421 tttcctgggt gggataatga ggcgcgcata cccggacgtg gaacaactta tatcgattcc
      481 tccccactca gatttcatca atggcttgct accttaatct cactttctca aaaacaattt
      541 aaaccgagtg agcagtttat ttttattaat gcttggaatg aatgggcaga aggcgctcac
      601 ctcgaacccg acagaaaatt tggatttagc tttcttgaaa gttgccggat tgctcagcaa
      661 ttagaaattt taagtcagca aaaaaacaac ctcattagcc aagaaaatta cccaaaagta
      721 gctatagtta ttcatgccta ctacccagat attttcgatg aaatacttga aaatttaagt
      781 agtacggaca aatataaaat caaattattt atcacaacac ccagctacca agtatcactc
      841 atagaaaatc gacttatatc tcacgggatg gagtaccaaa tcctaggtgt aaacaatcaa
      901 ggacgcgata tttttcccct tattaaaatc ttaaaagaaa tctaccagca acacttctca
      961 tttatagtaa aagttcacac aaaaaaatca aagcatagaa cagatggaac tatctggcgt
     1021 aaagatttat tttttaaatt acttacaaaa tctatgctag aaaaaaacat tcaatatttg
     1081 gttgatcatc ctgaagttgg tatcttagct ccagaaggtc atcttgttcc catgaaccac
     1141 tatatagccg caaatgaaaa agcaatcata gaattatctg ctcgattagg tgtcgaaatg
     1201 gaaaccgtta tgaagctaca tttcgtagca ggaaccatgt tcactgcgcg aattgatgca
     1261 ttgcttcctt tgctaacatt gagctttgag gatacagact ttggagtgga aaaggggcaa
     1321 ttggatggaa ccctggccca tgcacttgaa cgtttgatat caattggaaa tcaccgcata
     1381 ggctatcaga tcagaacact tagcggccaa accacgagcc attacgcgca tgccgacgta
     1441 acctccagat aaatattacc tgttttttga agaattatcg tttaaagaaa aatctactga
     1501 acgctgataa tcaatgacct gttttattcc ttcatgcaat ggagtcgttg gcttccaccc
     1561 taagatactt gctgcacgct ttatatctaa aacattaatc ggaacgtcgc acgcgcgact
     1621 ttcaagatga taatacgcga tatcatgctg aaatagctcc ttaataacgc ttaaaagctc
     1681 aagaatacta tgacccttgc cacccccaat gttgaatatt ttttcgggtc catcatagtc
     1741 aattagggta atcatcgcag aaatgacatc accgatataa agatagtcgc gaacaatgct
     1801 cccatccccc cagatttcaa caggcttatg atgtaatgct ttatgaacaa aagcagctat
     1861 gacgccctgg ctagaacgat gggactgaaa ttcaccataa ggattagcaa gtcgcagaat
     1921 actataattg agcttataca aataaccata gagtgcaagg tacttttcga tcgctaattt
     1981 ggctatacca taagcgctga gtggatcggt tggatgttcc tctggtacag gaattatgtg
     2041 aggaattcca tatattgttc cacccgatga aaggaaaata aaacgcggtt ttctcggttg
     2101 cttaattaca agctcaagta atttcagggt agcaccaaca ttctgctgta aatccaataa
     2161 aggattttca ttagatgttt gaggaatcgt tgtgctaatc aagtgaaata ctaaatcgat
     2221 cttatcgagg gcagtcatta catctgacac atgatccaaa tttcctgcag tccactcaac
     2281 accaggaata tctggccagt taccattgac tggctgcaaa tcaaaacctc taaccctatg
     2341 attcgcagca actaaagctg agcataggtg ttttccaata aaacctcttg ccccgagtac
     2401 aagaatgttc attggttact ttctagcgaa tacaacaaat aatcttcgat ctgattaatc
     2461 attcgattaa aagtaaactg attagcatga tctcgagctc cagaactaac agtcacccgc
     2521 aaattcttat tatggacaaa tttaactacc tgagcagcca gatgtctagc atctgctggg
     2581 cgaaacaata aacccgtttg ttcatgaaaa acaatttcag gattgccgcc aacatctgat
     2641 gcaatcgtgg gaatacccgc ccgaagtgca tgaattaacg taagagaaaa aggctcatat
     2701 aaagagggaa aaaggtaaat atcatagtct tgaaaaagac caaacagttt cgattcctct
     2761 actggtgcga taaagcaaac tctttcttct actccaagct ccgcaattat tttcttcaga
     2821 ttagccacat actgttgatc gcgcatatca cctacgatag ataggcttat tttcaaattt
     2881 ggtaacgcat ttattatatg gggcatggct tcgatcgctg tatggacacc tttgatatcc
     2941 actacacgtc cagcgaataa tagacgtagc tcatcgttag caataaacgt ttcgcgttta
     3001 gcaaacggta acgattcttc tactcctgat gaaataccgt gataaataat cttactgttt
     3061 gcaaactcaa acccagcacg agaataaaga ttttgcataa atcggctagc aaatatagcg
     3121 taacccagca tacttcgctt cttctgacca ttaaagagtc tacttttaaa acgatgccaa
     3181 caagcatacc aacgatcgcg ctttacataa acctcacgag aaaaataatt agcaacaaaa
     3241 tcactattat aaaaattgat aagccaatta tcagaaagca aataaacctg tttcttctta
     3301 accaatgaaa gtgcatcgag cagcgacaca tcaaaaaaat gcagattcca aataaaaata
     3361 agatctgggt ttatttcatc cagcgttcta gacaatgtct gcagattttt ttgattaata
     3421 ttgttgcgct cgatcgcaga aatattcaat ggtttataaa tattaccttc tgtggcaagt
     3481 aaagtaagta agcgctgtac ttgctgattc ggataaatcg cttgtttgcc gccataatca
     3541 ctggtcagca cgttgatttc atgccctttt tcagccagtc cccgcattac ctcaaagcac
     3601 agtacttcat agccaccgac tatattaggg gggtaaagat tactgatagt aagaattttc
     3661 atcgaatcgt gtcagtaaga aatttgcaaa aagcgttatc agcgagccaa tcatcaaccg
     3721 caagcctttc agtaacattc tgttgatagt atttaaaaaa gaaaaaaagc atccttgtca
     3781 aatcatggcg attggttatt tcgaccagtt gctcccaata gctccaatca tttcgcttca
     3841 tcttccaagc ataataggct accacagcag cccattccaa tttgactttg agaacccctt
     3901 cattgatggt attggtagca tgcgttcggt aatgcaataa tggattttct agaataacaa
     3961 ttcttttatt aaataaaatc gcccgcaaaa agaaatcgag atcgtgcgca taacgaagat
     4021 ttgcaaaggt acccagatca gcccataccg atcgatgcat aaataggtta gaggttgtca
     4081 tcaagaaatt accattaatc agtgctaatg aaagatcact gacttttcgg tgaaacgcta
     4141 gcgcttcttc ataccaagta ttgctacaag acttaccctt ttcattaata aaagcgagtg
     4201 ctgtacaaat tacatccgga acgggattgg cttgcattgc ttgcaggcaa aattcaaggc
     4261 gttgtggatg ataaacatca tctgaattaa gaatggctaa atactcacca gttgcatgat
     4321 taaggccatt attcagcgtg taatgtgcgc cttgattttt ctgcgaccaa gtaatgatac
     4381 gtggttcttc ttttgcgcga tgctgtactc gttgccagga attatcatgt gaaccatcat
     4441 caataatgat aatttcaaat ggaggtatcg tctgttgtaa caagctatca atcgcgtcat
     4501 caatatatcg ctcatggtta taaagcggta tgatcactga aattgtggga ttcaattcaa
     4561 gaacacggtt ttgcatatca atcattattt aaccgtttta ttttttaatc gcaaacgatt
     4621 aaatatttgg gtaaatcctt cttttgctac attcagtgaa aaattttgct gaacaaatgc
     4681 aagtccttca tttgatagct gttcccataa aatcggattg gaataaagat taataatgca
     4741 atcagagaac gccgaaggtg tatcagctat cagaatattt ttttcgtgcg tcaacgacat
     4801 accttcagca gctatgcctg tgccaatgca tggaataccg taagacatac aagtgatcag
     4861 ctttcctttt attccagcgc caaaccgtaa aggattaatg gatattctaa tttgattcat
     4921 taaatcagtt atatctggta caaagcctag aacaacaata ttgctactag ataaagcagt
     4981 aatttcttgt ggaggattac tgccaacgat gaaaaatttg attgtaggaa tttttttcaa
     5041 tacgtgtgga aaaatatctt gcacaaaata aagaactgca tctacatttg gacgatgctc
     5101 atagtttcca ataaatacca aatcattgcg ttgtgtaaag tcactcttac gaccaaacac
     5161 ctcgctgaca accggaatta cttcgatata aagattgggg ttttcttgca acaaaatttc
     5221 cttctcttta tgactcacca caatagtaca gtcagcttca gccgcgatat ttaactccat
     5281 tgctttgaga aagctggaat gggccaacat ctcgcgatta tgttcaacct cggcctgccg
     5341 ttgtaaacga ataaaatgca aatcatgtgt ttcataaatg aaatgcgccg tagtgcaata
     5401 ctttttgatt ataggtatca atgcttcggt atagacaggg cggcatgata aaacgaaatc
     5461 ataagaacga tctatcgcag ccaaatgatg ctctaatgca ttaacttgag gcttatttaa
     5521 acatcgtact tttagttcaa tcaatcgcgg tgtatagact ggatcataag ctaaatcaac
     5581 tggataaaat gttacaccat atcccatctc aataaaaact ttgataaagt tatagatcat
     5641 catagaacca gcatcctgat ctggtcgagg cacactgtgc tcaatcaaca ggatttgccc
     5701 agcaggtcca attggccagg gtcccccact ttgatcaatt tgagaactcg attgccttct
     5761 acgctgctga ctgaattccc taacaaaacg ataaattctg tatgcgcctg gaagtgtgta
     5821 atagcaccgc ttaaatacac tttttaatgc tctaacgcgc tccaaacagc agcgcaatgg
     5881 tagtgtgaga cgccaggaac gtgacgcata taaaatatct agctcctgct gtttttgctc
     5941 taattgaaaa cgtgtttgct gttgtaagtc ttgcaatctg ctaatttcac tgcgtaaccc
     6001 gcccatttca gcttgaaatt gattgttggt tcgatgatgc aaatcggcaa ccgctgcgaa
     6061 ttcgcttgct gtaagtaatt tcagccattt ttgatacagg ttaaccgttg cttgctttac
     6121 tcgaacagga tcattgtgca gcggagaaac ttccgatcca ccacccgtaa aataagtagc
     6181 gctcgttcga tttataaaat taaagggggt gtaccggcta agttgtagcc aaaaatccca
     6241 atcctcgtat aaagctagct tttcatcaaa cactgcgcct tgttttaaca acgttgctgg
     6301 aaagaggatt gcatggatag gaataacgtt ccctagcaac aattttgcaa aattgacttc
     6361 agccgtttta aattcaacaa acggcttatt ttcatcctgc tcatctttac cacgcacacc
     6421 ggtatatgca attgagcatg tcgatactga ctggattccc tcaatcaaat tagcaatatg
     6481 atccggatcg atcgcatcat catcatcaag aaaacttata tagctacctt gacaagcatc
     6541 taacccaata ttcgctgctt tagagcgaag caaaggttca ccatcctggt taattaaacg
     6601 aagcggaaaa tttccacacc aatcagacga ttgctcgtgc atcccgcctt ttgcattcac
     6661 caaaacaacc tcaatattgg gatacgtttg taacgctaaa ctatccaaag ctctcgttaa
     6721 cgtaggtcga tccatacttc gaataatgac actcactaaa ggaatatcac cacgatcgat
     6781 cgctactccc aactctcttt gacgcgcata ttcggattga taaagcttta atggatcctt
     6841 aaccaattct tcggccgaaa ttatcaaata ggcttccgct gcctgaattt cagctggcaa
     6901 agtataggtt cgatagcgat taagtgccgc aatatcgaga tcgtagcgct gcttctggtt
     6961 ttgcgagctg aagcactgca ttgcagccat tttacgctca acgaattcgc tgatatcgag
     7021 taagcaattg ggtcgcatcg gcatacccac ttcgtacaat gcgagcttca gtgatttacc
     7081 tattcttctg actgcttcaa tcgcagccat ccctatcgaa cggtgatcgg ggtgcacctc
     7141 gaaaacagag ggcgcataga ttaaatctgc ttgggattga ttgatagcag ctaaaatgtc
     7201 ttggatgagc ttttctccat agccaacctt acgatctgga tatgaccaga atgatggaag
     7261 accgtaccct aaaatctttg ctgctaagtt actttcttct tgacgctgta gcacatattg
     7321 ccttgaactt tcttccgatt ttgctccata cgcaccatca gagacgataa tcacctccac
     7381 agcaacgcct tgtgcaagat gccgcataat cgcgccgcca catccaaata cttcatcatc
     7441 ggggtgaggg gcaaacacca gcactttctt gcacggtatt attgttgtcg cttgataagg
     7501 tatgagaaga ttttccaatg agaatatttt ttatacaaaa attaatggat agtgcactac
     7561 ctaaaatcca tgtcaccact cattaaccac cagtgattca tgagtaattg tggtgatgga
     7621 ccatcactcc aagcatttgc gggtattcga atgggtaact cactcaacat tccttcacca
     7681 ctcgctgaaa taaaacgatc agagaaatta tgggtaattt ggcaaaatac acgttgatca
     7741 ccattaatcc gtgcaattcg tctcgtatga gttatcagca aggaaatctg atcgagtggt
     7801 gcaaccatat ccatgatttc acaaccattt gaattagatt ggagtaccag aattcccctc
     7861 gcttttctac tcaaccgact caggacgagc acaatacgat aatgatgttg aggatgattc
     7921 aaataacgat cacgtaggta ggcccaatcg cgtattccta taatcgctgc attaagatca
     7981 gatgccatac catgccaaca ctgattaatg gcatcgacta cccaattatc gttgatatta
     8041 tgctgatcga tgattttcaa ttgagaaagc cacaaaggtc gccttgaata aggagtccat
     8101 gctatttcca ccatacgccc cgtttcagca tacaatccgt gacgtttagc aactttcatc
     8161 gccctatcat tgggaaaacc aaatcctaat aaataaggtt taccataacc gatatagtgt
     8221 tccaggaatg ttgctgccat ctgaaaaaaa gggccttttt ttactaatcc aggatgattt
     8281 accctgtcaa ccatgacatc accgatttgg acggccattt gcggttggcc gaaataaagt
     8341 atgcttcgac ccattccacc ataatgcgca attagctcat tatcatacca aacacccatt
     8401 gctcgacatt gctccgaacc atacttccat tgccacattt ccgacgtcat cttgtgctta
     8461 aatattttct caaataacgc gagcatatgg ggcatttggt tggtgttaag ccgggacagt
     8521 cgccatggta attggcgttt ttttcgaaaa tgtaatagcg catagccaaa ttgacctgat
     8581 acatatttat ttagataatc ttgattggac tgcactaatt gatcgagacg actcgattcc
     8641 aaatcaagat catccagcaa ttgctgccga tgcttgccaa tggcatgcag taaatacttc
     8701 aatgtaggcg ttgcttttga ggataaatcc aaatgttcca ctaactcgaa gcctaaacga
     8761 tctgacaact gtaacatagc tttcaatcgg tgaagtcttc caatcgcagt ttccgattgc
     8821 tgcaatgcaa actcatcaat aatgaaaata tgcccttctt ctggtaataa atcttgcgct
     8881 ttgttaaata tcacgagcgg ttcgatatac tgagcggatt cctgaaacaa aatgacatca
     8941 taactttcag caataggctc caaatcctca taatgttgct gcgttacatt gagcccagtg
     9001 gcaaaccgtc gatgaatttc tgcaatttgc tgacgatccg gtgtaatact gtgaacttga
     9061 taaccctgtt gcgtcaacaa atgacaagtc gtacctaaac ctgcaccaac ctccagaatt
     9121 cgactagggg gaagtggtaa acgatccaga atcaaattgg ttgaatattg ttgtgcttgg
     9181 cttaaattaa tctgttctga ttcaaacaac ccataatgaa gataatctac cctaccttca
     9241 tttaaaaaaa gtgtgtgcgc atagacatta agcggaaaat cgaagttttt aaataacgat
     9301 gggctattga ttgtaataga cccatcactg atctcttgct tagatgaaaa tccattcatg
     9361 caattagata ttgaagacac gttaaacttt ggctgtaatt aaatagacga gatgatcccc
     9421 aaaataccat cactcaattt acttccattg atgaggaatc ataaaaaaac cttgtaatcg
     9481 attttgttgc tggacttgaa gtttcgtggc accgtaaaca gaatcataga gatgaattcc
     9541 ctgctcacat aataagtaca cactgactaa atattcacct ttgagtaaag gaagttgttc
     9601 aaagaggagt tttatttccc ctattccttc tggcgttaat cgagggatga tactatcttc
     9661 ccaactcgca gcgcttgtta ttactcgacc atccatagaa tgaagtgtaa ctgcaactgt
     9721 cggacagggt aacgagggat ccgtagaaaa tttgacatcg acctggatcg ccgattgccc
     9781 actttcggct accgccacag cgcagggtgc tccatcaatt tcaacgacca ctgtttcaag
     9841 ccttgcatag ccctttgcat tagcaagata atcattctgc tgaatagtcg ccgcaatatc
     9901 attattatcg ctgcttaata cacccagatc caggaattgt tgataagccg taacaacttg
     9961 ctttacatca ccttctgcta tcaattctcc ttgattgatc caaaatgcac gattacacaa
    10021 tgactcaatc tgaaaaagtg agtgggagca aaataaaatc gttttgccgc tatcccgtaa
    10081 ctgcatgata cgattaaatg atttgcgcgc gaaagcgcca tctccaactg acaaagcctc
    10141 atcgataatc aaaatgtccg gatcaacact gattgcgatc gaaaaagcca accttacata
    10201 cattccgctg gagtaagttt taacgggttg atcaacaaaa ggacgaattt ccgaaaaatc
    10261 aataatttgt tcaattcggt cggcaatttc ttgttgtgtc agtcctatta ttgctgcatt
    10321 catccagata ttttctcgac ctgtaaactc aggattaaag cctgcgccca attcgagcaa
    10381 cgcagctaaa cgaccattga tctctacttc tcctgttgtc ggtgttagcg ttccacaaat
    10441 taactgcaat aaggttgatt tacccgaacc atttttccca ataattccga tcacttcacc
    10501 tggcataatt gcaagattaa tctcacgcaa tgcccacaac tctcgataat actgacgacg
    10561 ccgaccccag atcaattgtt tcaaacgatc tttgggctgt gtataaagtt gataacattt
    10621 actcaaatga tgagcaagaa cggcaggttt aagtgtcaca gtatttgatc atgaacatac
    10681 aaggcttgat tgaaattacc cttaaagcag tttccagtat aaattgtttc atgatttaac
    10741 caatgaatta gcttatttta acttctaaag ttattaacaa gttcattact tcaataaaat
    10801 taactaaata cgttcaaaaa caaagaatta accgatcatt gcgatgaact accaacgtga
    10861 aatcaaacat cagtgtagaa tgtatataag cacgtttatt gagtccaatt cacatcgtta
    10921 gtccatgcag gaatttgaag cattctggta tacatgtctt aagcattttg agaaggaatt
    10981 aaatgctcag caattcaata catggattaa accattgcag ctcgatatct cggaaggttg
    11041 tggcgaccaa cctattttga ttgctcctaa tcggtttgta cttcaatggg ttaaagacaa
    11101 ctttatccct catatcgaag ctatggcgca gcaacatttt tctaaaagga ttcaatttca
    11161 actcgtgctt ggtgaaatca ctaccaaaaa agctactcct aaagtaaacc cgatcatcga
    11221 cacctcacgg gaagatcaag cggtttcaac cgtttccgtt gctgatccga caccctccaa
    11281 aaaagcgagt aataagcatt atctaaaccc gaattttact tttgataatt tcgttacagg
    11341 aaaagccaat caactggcgc gagctggcgc tatccaagtc gctgaaagac caggtactgc
    11401 ttataatcca ttatttatat atggtggcgt tgggctagga aaaactcact taattcaagc
    11461 gattggtaat tttatcttag aacgcaatca cgaagcgaaa attagatatg tccatgcaga
    11521 gaaatatgtt tcagacgttg taagtgctta ccaacataaa tcgttcgaca aatttaaaaa
    11581 atattatcat tcccttgatg tactattagt ggacgatgtt caattctttg gtggaaaaaa
    11641 tcgaacccaa gaagaattct tctatgcttt caactcttta attgaagcat ccaaacaagt
    11701 catcattaca tgcgattgtt atccaaaaga aatctcagga ttagaagaac gactagtatc
    11761 gcgatttggc tggggtttaa ctgttgcgat cgaaccgccg gaattagaaa tgcgggtcgc
    11821 aatcttaatc aaaaaggcag cgctagaaaa aatttcacta gaagaaaata tcgcattttt
    11881 cattgcgaaa cacattcgtt cgaatgttcg tgaacttgaa ggcgcgctga aacgcgtatt
    11941 ggcttattct cgttttacag gccaggattt atccctggaa ttagtaaaag aagccctaaa
    12001 agatcttctt gccgttcaaa acagacaaat ctcgattgaa aatatccaaa aaactgtcgc
    12061 ggattactac aaaatcaaag ttgctgaaat gtattctaaa aagcgaacgc gaacgatagc
    12121 aagaccgaga caaattgcca tggcaatcgc aaaagaacta acacaattaa gcctgccgga
    12181 cattggtgaa gcttttggtg gcagagatca cactaccgta ttacatgccc accgcaaaat
    12241 caatgaatta cgcgcatcag atcctttgat tacccgggat tacaatgcgc tgattcatat
    12301 tttgagggga taataactaa ggaataaaac ttaataatag tgatgtatca attgtggata
    12361 actcatcatt taaaaattaa ataagaatta tccacaattt atccctttta tatttttaac
    12421 ttatacagcc aatattattt aaacatgtct ttgattttta atatttatta tttcatatcc
    12481 ccgaattaag ccctctttat ctattattat ttagttttaa attaaatgaa tttaattaat
    12541 acaaaacgtg acacattact taaaccgcta caaattgtaa gtggcattgt tgaacaaaaa
    12601 cgaaccttgc caatactttc aaacgtattg attcgtaagg aacaagaaaa aatttttttc
    12661 gttaccactg atctggaagt ggaaatcgaa gtacactcga tcgataatga aaatctttcc
    12721 aaagaaaact tttcaataac agtatcagca agaaaattcc tcgatatcct tcgcgcattt
    12781 tctgctgata ccagtgtcgt tcttaacaaa actgataatc gcttacaagt taatatcagc
    12841 aaaagtcgct tcaatcttca aattcttccg atggaagatt ttccaagaat ggcgatcgaa
    12901 gaaaagccag aggtttccct aacgttgtcg caaaaagatt taaaaaattg cttgtaccat
    12961 gttcaatatg caatggcaca gcaggatatc cgctactatt taaatggttt gttactgcta
    13021 actgacggtc aacaacttaa agctgttgca accgatggtc accgccttgg ctatatctcc
    13081 atgcagtttg aagcggagct aatcaaaaaa gaaacaatcc tcccccgtaa aacggttcat
    13141 gagcttgtaa aattactcga agatagtgat aagacggtaa cgattgactt ttattctaaa
    13201 aagatcagct ttagcttttc tggcatcatt ttaatttcta aaatcattga tggaaaattc
    13261 ccagatttcg accgtgttat cccaaaaaat aatacaaagc agtttataat caaacgcctg
    13321 gattttctcc aagcacttca gcgcgctgcc attctttcta atacgagtga taaatttaga
    13381 ggcgtgaaac ttattattgc aaataatcgt ttaagcatcc tttgcaaaaa tagtgaacaa
    13441 gaggaagcgc aagaagattt ggatattgac tataatagtc aggaaatcat cgacattagc
    13501 tttaacatta cttatttgct cgatgtactt aataatctcg attcagagac aattcaatgt
    13561 tcctttgaaa atgcaaatag cggcacacta atcaccattc caggtgatga agctttcaaa
    13621 tatgtcgtaa tgcccatgcg actctagtaa ttcaatttga tcaactctta aaagcacgac
    13681 tgacaaaatg accaataaag acgatcgaga atatgattcg gacagcatca aaattctgaa
    13741 aggattagat gctgttcgta agcgtcctgg aatgtacatc ggcgatacca gcgatggaac
    13801 aggcttgcac catatggtat ttgaagtcgt tgataatgca atcgatgaag cattagcggg
    13861 acattgtgat gaaatcattg tcatcattca tcccgacaac tctgtcagcg tccatgataa
    13921 tggacgtggt attccaactg gaatcaaaca agatgatgag ttgaaacgct cggcagccga
    13981 aattgtcatg accgaattac atgcgggcgg taaatttgat gacaattcct ataaagtttc
    14041 tggtggctta catggtgtcg gtgtttctgt cgtgaatgca ttgtcggaat ggctcagact
    14101 aacgattcgg cgtgataaga aaatccatca aatggaattt cgaaaaggtg ttcctgtcgc
    14161 tccattggca gtaacaggtg aatcagaaaa gcatggaacg gaggttcatt ttttagccag
    14221 taaggagatt tttggtgatg ttaactatca ctatgaaatt tttgctaaac ggttgcgaga
    14281 actttctttc ttaaataacg gaattaagat caagctcatc gatcaacgcc atgacaaaga
    14341 agaaacattc gctttttcag gcggtattcg taattttgtt gaatatatca accgcactaa
    14401 aactgtatta catccagcaa ttttttacgc gaacgggagt aaagacaata ttgcggttga
    14461 ggttgccata cagtggaacg atagctatgt agaacaagta ttgtgtttta ccaataatat
    14521 cccgcaaaaa gacggcggta ctcacttaac ggggctacgt gctgccatga cccggacgct
    14581 taatagttac attgaaaaaa atgaattagc caaaaaagcg aaagttgata ctaccgggga
    14641 cgatatgcgc gaaggtttaa cctgtgtttt atcaatcaaa ctatttgaac cgaaattttc
    14701 ttcacaaacg aaagaaaaac tcgtttcttc agaagttcgt cccgtcgtag aagatatcgt
    14761 tgcacaaaag cttactgaat ttttgttgga acatcccagc gataccaaaa ttatctgcaa
    14821 taaaatcatc gatgcggcaa gagcacgcga ggcggctcga aaagcacgtg aattaacccg
    14881 aagaaaaggg gttctcgata atcttggttt accgggtaag cttgcagatt gtcaggaaaa
    14941 agatccagct aactgtgagc tctatcttgt ggaaggagat tcggcaggtg gttctgcaaa
    15001 gcaaggcaga gaccgtaagt ttcaagccat catgccttta aaaggtaaaa ttttgaatgt
    15061 cgagaaatca cgatttgata aattaatttc ctcacaagaa attatttccc ttattaccgc
    15121 gttgggaacc gggattggca aggatgaata taatccggat aaattgcgat accaccgcat
    15181 cattatcatg acggacgctg atgtcgatgg ctctcatatt cgcaccttat tattgacttt
    15241 cttctaccgt caaatgcctg aattgatcga acgtggtcat atttacattg cgcaaccgcc
    15301 gctttataag atcaagcatg ggaaacaaga gcgctacgtt aaagatgatc atgaactcaa
    15361 tcaatatgtc ttgggattgg cgctggaagg cgctgaatta cagattagtg aaagcaatcc
    15421 accgataatg ggagaagctt taaccagcat tgcacgagaa tatctgctgg ctgaagctgt
    15481 tatcgaccgc atgagccgat tgatcgatcg ttccgtcatg tatgctttgc tcaaggaacc
    15541 tgacatcgat ttaagcaacc aagataatgc acagcaaagc gcagcaaagc ttgccgcaat
    15601 tgttcaaact gttcaaatca accctgaatt tgatgatgtt ttcgagcaat atcgcttaaa
    15661 gttgatccgc acccatcatg gaaatattca aaccagttac ctcgatcaag attttgtcaa
    15721 aagcaatgat ttcgagcaga tcaaaaaagc tgcacaagtc ttacaaggtt tgataacaaa
    15781 tggagcagta gttaagcgcg gcaatgatga gcataccatt acagaattta aagaagcact
    15841 cgattggtta cttgctgaag ctaaaaaagg catcaatatt caacgctaca aaggcttagg
    15901 agaaatgaat cctgctcagt tatgggaaac gaccatggat ccgaataatc gtcgtctact
    15961 gcgcgcacaa attgaagata gtattttgac ggatgagata tttacgactt tgatgggtga
    16021 tgtcgttgag cctcgccgtg aatttattga aaaaaatgcc cttcaagtga caaatttaga
    16081 tgtttagttt cgaaattaac gctcgaaaat gactcgagtg aaatttagat aatgataaag
    16141 ggttttataa agtaccatga attggtataa tgccaaatta cgggtcgtta gctcagccgg
    16201 tagagcagcg gacttttaat ccgttggtcg cgcgttcgaa tcgcgcacgg cctaccaata
    16261 aaatcaaagg gttagcaaaa tcgctaaccc ttttttattt tctggggtta cgcgggggtt
    16321 acattacaaa attaactttt gatagccgct ctctagaaaa actatttatt tgcaatactt
    16381 ctcatagaat tcctggctat attcttcggg agtaatcgtt ttatctatat ctgccatttc
    16441 atttgcctat atttcccaaa ccgggtatag actggatcaa gagatgaatg ttgaggttgg
    16501 ttggcggaat tgaagcgctg ggatcagcag gacagctcca atgtcgcctt tgttggctgt
    16561 tttggcggtg cggtatcacc agttcggact gcgtggcaaa ttgaaaatat cctcgtctat
    16621 gccaaggaaa agcataggac tacaaaatcg acaataatcg gctgacggat catcatcatg
    16681 acgcactagc aaatcacgca atagtaatac gcgcgccggt ctttactaat ggggggcccc
    16741 gattatttgt aaataaaatc aaatgttttt ttgatcgttt agcagcgtat ttcctaaaca
    16801 tccgatttgc g
//
LOCUS       DAOQLJ010000036        16391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1277_length_16391_cov_6.878061, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000036 DAOQLJ010000000
VERSION     DAOQLJ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 16391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16391
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1277_length_16391_cov_6.878061"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PK522_04180"
     CDS             <1..189
                     /locus_tag="PK522_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40962.1"
                     /translation="YTFVIALPMELTGLSSSLNKPIPVNNDVNAWVCQGEKCLSEIVD
                     LQELLQVCKDKGKMAFPL"
     gene            212..523
                     /locus_tag="PK522_04185"
     CDS             212..523
                     /locus_tag="PK522_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNC40963.1"
                     /translation="MRNVWLSVLAASAVLLVGNVQADADLAKNSGCLNCHSVDTKLVG
                     PALKDIAAKYAGQDGAADMLAGKIKNGSNGVWGAIPMPPNANVSDENAKVLADFILTL
                     K"
     gene            complement(575..1294)
                     /gene="flgA"
                     /locus_tag="PK522_04190"
     CDS             complement(575..1294)
                     /gene="flgA"
                     /locus_tag="PK522_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNC40964.1"
                     /translation="MNGDEGMKKERILAFIGGILLLLSELVFGATTQSISSLLESAEN
                     FAYQGTRDLPGSVRITLNKMDSNIALPKCSRLESFLPTGSRLWGKTSIGVRCNDQQAW
                     TIYIQVDIEVITKVIHSAKPILRDHTINEGDIVFKEVNLVRMSEGAFTDSEKVIGKVA
                     VTNINVGQPIYSQSVRAPYIIQRGQKVKLIVEGSGFSVSTEVEALSAAAEGQILQIRN
                     QSGRIMTGIARQDGIVEIKRK"
     gene            1526..1936
                     /gene="flgB"
                     /locus_tag="PK522_04195"
     CDS             1526..1936
                     /gene="flgB"
                     /locus_tag="PK522_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNC40965.1"
                     /translation="MINKLNKTLDFYHKALNVGAARHELLSSNIANADTPKFKARDVD
                     FMKTLQNAMTFNPSNMTTTSTMHMNGDRGFRSIASSELLYRIPQQPSIDGNTVDMDTE
                     RMQFINNSIKYDAGLTFINNQFRFVLSAIREGNA"
     gene            1939..2343
                     /gene="flgC"
                     /locus_tag="PK522_04200"
     CDS             1939..2343
                     /gene="flgC"
                     /locus_tag="PK522_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNC40966.1"
                     /translation="MSLFNVFNIAGSAMAAQSQRLNVVASNLANADSVASSNGQPYRA
                     RQVVFSTLSTATKEAKGVKVAGVIEDKSPMKVVFDPKHPMADAKGYVTMPNVNVVDEM
                     VNMMSASRAYQNSVNLMNTTKALLQKTLTIGQ"
     gene            2355..3029
                     /locus_tag="PK522_04205"
     CDS             2355..3029
                     /locus_tag="PK522_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HNC40967.1"
                     /translation="MTQVQNSNPITSSFLDSVKTSNNTGKKEEIDPQDRFLKLLVTQM
                     KNQNPLKPLDNAEVTSQIAQISTVTGIEKLNTTLQSLVSDANSRRSLEAATMIGREVL
                     VPGSIMKLEEGEGLAGIELTEAADKVTMTITDSSGVKVREIDLGAKAAGINPIIWKGD
                     TESGAAAANGNYKFTVSAWRDGQEINVTSLAVGKVESVSTSDKGPTLDVGDLGHVNLS
                     DIKQIL"
     gene            3042..4319
                     /gene="flgE"
                     /locus_tag="PK522_04210"
     CDS             3042..4319
                     /gene="flgE"
                     /locus_tag="PK522_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HNC40968.1"
                     /translation="MGFQHGLSGLRAASTNLDVIGNNVANANVIGFKQSQAQFSDIFA
                     NSLGGAGSNQVGIGTQVNTIAQSFSQGNISNSTNPLDMAIVGGGFFRLSDNGAISFSR
                     NGQFHLDKSGFLVNADNLNVTGYPADKNGKIIVSGPTNLQLSTAEMTPQATTTFETGV
                     NLDSRTLVPATLPAFDATDPDTYARSTSGTIFDSLGNSHVFTLFFQKSAPSTWDVYAT
                     VDGAVDASGVPVGVQLDGGSPLTLTFDSNGVMTGPTGSVTASVDLSAVATNLGIADWG
                     ATTPLDFDFDITSSTQFGSNFAVNKLSQDGFTSGRLAGYGISDDGTIQGNYTNGENRT
                     LGQLVLASFANPNGLKPQGNNQWVETAASGLPLIGVPLTGTLGEIQSAAVEEANVDLT
                     TELVNLITAQRVYQANAKTIETQDAVLQTLVNL"
     gene            4372..5118
                     /gene="flgF"
                     /locus_tag="PK522_04215"
     CDS             4372..5118
                     /gene="flgF"
                     /locus_tag="PK522_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HNC40969.1"
                     /translation="MDRLIYTAMTGAIHTMTQQATTAHNLANVSTAGYRAEVDSFRAV
                     PVYGDGLPTRAFVLDSTTGADFTPGPIHRTGRDLDVAVQGSGWIAVQLENGEEAYTRH
                     GNLQVSPNGLLTTTQGGAAVKGEGGAISLPPDSRVAIGKDGTVSMIPNDPRVTAVIVV
                     GRIKLVNPPPEQLKKGLDGLFRLKEGKEAPVDPNVVLVEGAIEGSNVNVVHEMVNMIS
                     LARQFDMQMKILDSAQQNSQRASEFMVYSG"
     gene            5151..5933
                     /gene="flgG"
                     /locus_tag="PK522_04220"
     CDS             5151..5933
                     /gene="flgG"
                     /locus_tag="PK522_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNC40970.1"
                     /translation="MIRSLWISKTGLDAQQTKMDVIANNLANVSTNGFKRMRPVFEDL
                     LYQTIRQPGAQSSQQTRLPSGLQIGTGVRPVATENIMLQGNFSQTGNTRDIAIKGEGF
                     FQIMLPDGTFAYTRDGAFQSDDQGQVVTSSGFVLQPPVSIPPNATSITVGRDGTVSAI
                     LPGATLSTQIGNIQLVSFINSAGLQRAGENLYFETVSSGPPNPNIPGTNGLGLLYQGF
                     VETSNVNVVEELVEMIQTQRAYEINSRSIQTSDQMLGRLTQL"
     gene            5989..6669
                     /locus_tag="PK522_04225"
     CDS             5989..6669
                     /locus_tag="PK522_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNC40971.1"
                     /translation="MLGLMIIIIGAAGCSLLTPAPKVHQPMTMQPHPVPSITPREANG
                     SIYHVAYSHPTQRYIPLFEDRRARGIGDTIIVNLNERTNASKRSASNIGRESQTSFSI
                     PSFLGLPLKKIFQGVDLETESENKFDGKGASSSNNNFTGTITVTVVDVLPNGNLLVSG
                     EKQVGINQGHEFIRLSGVINPINIMNNTVSSIQVADARIEYRGSGYLDEAQTMGWLSR
                     FFLTVLPF"
     gene            6724..7839
                     /locus_tag="PK522_04230"
     CDS             6724..7839
                     /locus_tag="PK522_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNC40972.1"
                     /translation="MLIFRRWILWFSLINCLILPSYSFAERIKDLASIQGVRANQLIG
                     YGLVVGLDNSGDMTTQTPFTVQSIVSMLGQLGINLPPGTNLMLRNVAAVMVTATLPAF
                     SRPGQLIDVTVSSMGNAKSIRGGTLLMTPLKGIDNQVYAVAQGNVLVGGAGGSAAGSS
                     VQVNHLASGRIPGGGIVERAVESALGQGEFVNLELHSTDFTTARRITEAINRIFPHGT
                     AYSVDGRVIQLKAPQDVNQRVAFISQIESLDVTPAQGIAKVIVNARTGSVVMNQTVTL
                     EASAVAHGNLSVIINSQPIISQPGAFAERGETVVTSQAQIEVRSDEGNLMMVQSGANL
                     AEVVQALNTIGATPQDLLAILQALKAAGSLRAELEII"
     gene            7865..8896
                     /gene="flgJ"
                     /locus_tag="PK522_04235"
     CDS             7865..8896
                     /gene="flgJ"
                     /locus_tag="PK522_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HNC40973.1"
                     /translation="MKLLNTFDTSTQLAIDTKGIDNLRLQARQNEDEGLKQTAKQFES
                     LMVNMMLRSMRSATPKNGIFDSEQSQFYTQMLDQQLSQNLSSQGIGLAEMLVQQLKKD
                     SSGNLIDHPISEAESILSSIESSAGKNGLISLAPSEPKDLPNSLWTTPKQETNRPISM
                     FQSYQSTVMPLEVNKSNASKSMQSLQGKPNDFVNLVQPYARAIAKETGIPEQFMIAQA
                     ALETGWGKHQIRLSENQPSYNLFGIKAGKNWKGPVAEAVTTEYINGVAQKVVDKFRVY
                     RSYEESFRDYANLLLTNSRYSGVLKTQSAVAFAWGLQQAGYATDPYYAQKLIKILNSE
                     VFQINKIPS"
     gene            8991..10904
                     /gene="flgK"
                     /locus_tag="PK522_04240"
     CDS             8991..10904
                     /gene="flgK"
                     /locus_tag="PK522_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNC40974.1"
                     /translation="MGNSILNIGVSGLQAAQAALLTTSHNISNAHTPGYNRQQAIQST
                     NIPQPAGTGFLGQGVHISTVQRVYSEFLYSQGLQAQTQSSQLDSYYTQIKQVDNLLAD
                     TTSGLTPALHNFFKAVHDVASNPSSVPSRQSLLSNAEALVGRFQSMDQRLSEIREGIN
                     SQITSTVDEVNSLAKEIATLNINIQAAEGSTNGQPVNDMLDQRDTLVRQLSELVNVTV
                     VRQDNGHHNVFIGNGQALVVGSQSMALKALPSPDDPGKITIALDAGNNTILLPEKQLQ
                     GGSLGGMLDFRQNSLDKAQNTLGLIAIGLAQTFNDQHHLGQDLNGALGEDFFEVPEPK
                     VISLAGNSSTSTITASISSVGDLNTSDFQFNYDGSQYTLTRLSDNVSVSSATAPSIGS
                     PLTLSGISVTAATIQPNESFVIQPARNGAKDIATAINDTSKIAAAAPIRSQAASTNTG
                     SATISAGIVNPLPIDPDLSQSVTIKFHTPSDGMFDVTGTGASLPAMNQTYTEGADISF
                     NGWTVQISGVPEAGDEFTISPNTGGIADNRNALLLAGLQTKNTLQDGTASFQSTYAQL
                     VSQVGNRTRELEVTSKAQANLLAQTDQSIQALSGVNLEEEAANLLRYQQAFQASGKVI
                     EVSHSLFDTLLRI"
     gene            10939..12165
                     /gene="flgL"
                     /locus_tag="PK522_04245"
     CDS             10939..12165
                     /gene="flgL"
                     /locus_tag="PK522_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNC40975.1"
                     /translation="MRVSTNTMYDQGVQSMLKQQEALFKLQQQISVGKRIVTPSDDPV
                     AAARAHEVSQAASINSQYAENRDKAKASLGLVESNLQGVSTIIQNVRSIAVAAGNPIL
                     SESDRRVMAIELRGRLEELIGLANSADSEGNFLFSGYQAKVQPFVKNAGDVQYAGDQG
                     QRLTQVTSSRQIAVSDTGANIFERVMDGNGVFTTSANATNIGTGIIGVGSVTDPTNLT
                     GDDYEINFHVTQDPLTLKDIITYDVLNTTTSSTISTGNTFVDGNMIAFDGLQFDIKGK
                     PADGDQFKVEPSSQQSVFKTIDNLINVLETTTNDQSGKTNLTNSLNTAMQNLDQGLDN
                     VLSIRASVGVKLQEIDALQSIGEDNALQYDQTLSRLQDLDYAKALSDFTRQQGLLEAA
                     QQSFIRISGLSLFNRL"
     gene            complement(12181..13233)
                     /locus_tag="PK522_04250"
     CDS             complement(12181..13233)
                     /locus_tag="PK522_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNC40976.1"
                     /translation="MDSKHSIDRHFLFWVLLIAVSGFLTYLLSPILTPFLLAAVIAYI
                     CNPFVSWLNTHNISRTIGTILVMVLSLAIFTALILVMMPLFEREILRLIERTPEYLET
                     IRETGIPWLKSTLGIDLQLDIAVLKQAFTEHWKSAGGIAAKMLPSLTSGGMAILDFIM
                     NLLLVPVVLFYLLRDWNSLLAEFNEWIPSIWKKQIYPMVREADRILAEFLRGQIAVIL
                     LMCIFYVTGLWLTGLEFSLPIGLVAGILVFVPYLGAIVGLSLATFAALVQFQDWGDII
                     WVLVVFGSGQLLESMLVTPWLVGDRIGLHPVIVIFALLAFGQLFGFIGILLALPISAV
                     LLVWLRHLHQQYLNKT"
     gene            13295..14356
                     /gene="purM"
                     /locus_tag="PK522_04255"
     CDS             13295..14356
                     /gene="purM"
                     /locus_tag="PK522_04255"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNC40977.1"
                     /translation="MTSSDSKYSTADQLSYRDAGVNIDAGNLLVERIKPFAKRTWRPE
                     IIGGIGGFGALFEISNKYRQPVLVSGTDGVGTKLKLAFHLNRHDRIGIDLVAMSVNDI
                     LVQGAEPLFFLDYFACGKLNVDIAAKVIEGIAVGCEQAKCALIGGETAEMPGMYPEGE
                     YDLAGFAVGVVEKDQIIDGTSIAENDVILGMASSGAHSNGFSLIRKILEYHPVDSDSK
                     LVDDVKLLDAIMEPTRIYVKAILELKNHIPIKGLAHITGGGLLENIPRVLPANLRAVL
                     HKGSWAVPPLFTWLQAHGNVADEEMYRVFNCGIGMIIVLAPEYVDEALAVLKTYNETV
                     WQIGTVQPNPTNDQRVLIV"
     gene            14368..15003
                     /gene="purN"
                     /locus_tag="PK522_04260"
     CDS             14368..15003
                     /gene="purN"
                     /locus_tag="PK522_04260"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNC40978.1"
                     /translation="MQSLVILISGRGSNLQALITANLPVKSIVVISNNPSASGLAVAQ
                     SHQIATHVIDHRCYLDRDSFDAELARQIDRYQPDLIALAGFMRILGDKFINRYHGKLI
                     NIHPSLLPSFPGLNTHARALEQGVKIHGCTVHFVTNQLDHGPIIIQAAVPVFPDDMPD
                     MLANRVLKQEHLVYPTAVRWFLESRIKLKDGQVKISSALNVDQTLYSPTID"
     gene            15097..16356
                     /locus_tag="PK522_04265"
     CDS             15097..16356
                     /locus_tag="PK522_04265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNC40979.1"
                     /translation="MRLTTIQLEMAIQAIRKILPLNFPADILMRGFFRENPMLGHNDR
                     AIIAEIVFGILRHKYFLDTLAEKATPRALLLAYLAKFQGINSRELISFATESEIKWLA
                     HVKGAQIAMQPLAIQAELPQWLIEKLQTYLPDEEILKLGVALQQPAPLDLRVNTLLAK
                     REEILTQLEQAEVKANAMPYSPIGIRLAEKTAINQHPLFLEGKVEIQDESSQLSVYLL
                     APRRGEMIVDFCAGSGGKTLLIGALMQSHGRIYAFDVSDKRLNNLKPRLKRSGLSNIH
                     PIRIDNENDLRVKRLAGKIDRVLIDVPCSGLGTLRRNPDLKWRQSPESIEELKIKQRA
                     ILNVASKLLKPGGRIVYATCSLLPEENEEIVESFLANHTQFIALNCGELLAQQKIPLN
                     TGNYLQLSPANHHTDGFFAAALELKKN"
BASE COUNT         5037 a         3106 c         3613 g         4635 t
ORIGIN      
        1 tatacttttg ttatcgcttt gccgatggaa ctaactggtt tgtcgagtag tctgaataaa
       61 ccaatacctg taaataacga tgtcaatgca tgggtttgtc aaggtgaaaa atgcttgtca
      121 gagatagtag atttacagga attgctgcaa gtctgtaaag ataagggtaa aatggcattc
      181 cctttataat tttcaaaata aggaatatac gatgagaaat gtttggttaa gtgttttagc
      241 tgcatcagca gtattattgg taggaaatgt acaagcagat gctgatttag caaaaaatag
      301 cggttgctta aattgccata gtgttgatac caaattggta ggccctgctc ttaaagacat
      361 cgcagcaaaa tatgcgggcc aagatggtgc tgctgatatg ctagctggaa aaatcaaaaa
      421 tggtagtaat ggtgtatggg gagccattcc aatgccaccc aatgcgaatg ttagtgacga
      481 aaatgctaaa gttttagcag actttatttt aacacttaaa taattctagt ttactgagtg
      541 tgataaagcc gatgtaatga ttacatcggc ttttttattt tcgttttatc tcaacgatac
      601 catcttggcg cgctatccca gtcatgattc gacccgattg attgcgtatt tgtaaaattt
      661 gtccctcagc tgcggcagaa agcgcttcaa cctcagtact gacactgaaa ccagacccct
      721 ctacaatcaa cttgactttc tgccctctct gaatgatata gggagctcgt accgattgac
      781 tatatatagg ttgtcccaca ttaatattgg taactgcaac tttaccgatt actttttctg
      841 aatcggtgaa tgctccttca gacattcgta ccagatttac ttctttgaat acgatatcac
      901 cttcatttat ggtgtgatca cggagaatgg gttttgcaga atgaattact ttagtaataa
      961 cttctatgtc gacctgaata taaattgtcc acgcttgctg atcattacag cgaacaccga
     1021 tagatgtttt tccccatagc cgacttccag taggtaggaa ggattctaat cttgaacatt
     1081 ttggtagtgc gatatttgaa tccattttgt taagagtgat cctaaccgac ccaggaaggt
     1141 cgcgagtgcc ttgataagca aagttttcag cggactctaa gagggatgat atggattgag
     1201 tagttgctcc aaaaaccaac tcggaaagta ataataaaat tcctccaata aacgccagaa
     1261 tccgctcttt tttcataccc tcatcaccat tcatggtgta aatgttctag aacaattgct
     1321 acacaagaga atttttttgc cggtcatggc aatttttgcc gcctgcaaaa gatagtagcg
     1381 ttaaattccg gtgcctaaat cgtgaactga agactaattt ctacttattt agaatatttc
     1441 gttgtgtttt taaagcaaga aagactggca tggttaatgc gtatataaga aagcagaaag
     1501 ataaaacttt ttaggaaaga aagcgatgat caataagcta aataagacac ttgatttcta
     1561 tcataaagcc ttaaatgtcg gtgcagccag gcacgagttg ctatcaagca atattgcgaa
     1621 tgcggataca cctaaattta aggcaagaga tgttgatttt atgaaaacgc tacaaaatgc
     1681 catgacattt aatcctagca atatgacgac aacttcaacg atgcacatga atggtgatcg
     1741 tggttttaga tcaattgctt cttcagaatt gttatatcga attcctcagc agccaagtat
     1801 agacggtaat acggttgata tggataccga acgcatgcag tttatcaaca actcgatcaa
     1861 atacgatgct gggctaacgt tcatcaacaa tcagtttaga tttgtattgt cagctatcag
     1921 agaaggaaac gcataaaaat gtcactattt aatgtcttta atattgctgg ttcagcgatg
     1981 gctgctcaat cacaacgact caatgtggtc gcaagtaatt tggcgaatgc agatagcgtg
     2041 gcaagttcaa atggccaacc ctatcgcgcg cgacaagttg tatttagcac attgagcact
     2101 gctaccaaag aagcaaaagg tgtaaaagtt gcgggtgtta tcgaagataa atcacccatg
     2161 aaggttgttt ttgacccgaa gcacccaatg gcagatgcaa aaggctatgt caccatgcct
     2221 aatgtcaatg tggtagacga aatggtcaat atgatgtcag cttcgcgcgc ttatcaaaat
     2281 agcgtgaatt tgatgaatac cacaaaagct ttgttgcaga aaacgttaac tatcggtcaa
     2341 taagaggaac agctatgact caagtgcaaa attcaaaccc aattacatcg tctttcttgg
     2401 actcagttaa aacatcaaac aatactggaa aaaaagaaga gattgatcca caggatcgat
     2461 ttttaaaact actggttaca cagatgaaaa atcaaaatcc attgaaacca ttggacaatg
     2521 cggaagtaac cagccagatc gcacagatta gtacagtaac aggtatcgag aagcttaata
     2581 ctacgctaca aagtttagtg agtgatgcaa actcaagacg gtctttagaa gcagcaacca
     2641 tgattgggcg cgaagtgtta gtacccggca gcattatgaa gctagaagag ggggaaggcc
     2701 tggctggtat agagctaact gaagcagcag acaaagtaac gatgaccatc acggatagct
     2761 ctggtgtcaa agttcgtgag attgatttag gagcaaaagc tgctggtatc aatcctatta
     2821 tttggaaggg tgatactgaa agtggtgctg ctgcagccaa tggtaattat aaatttacag
     2881 ttagtgcatg gcgcgatgga caagagatca atgtgacgtc attagcagtt ggcaaggttg
     2941 agagcgtatc taccagtgat aaaggaccta cattggatgt gggagatctg gggcacgtta
     3001 atttatctga tattaaacaa atactctaag aggagcacaa aatgggtttt caacatggct
     3061 taagtggctt aagagcagca tcaacgaatt tagatgtgat aggtaataat gtggcgaatg
     3121 caaatgtgat tggatttaag caatcccaag cgcagttcag tgacatcttt gcaaactcac
     3181 tcggtggcgc aggaagtaat caggtcggga ttggaactca agtcaacact attgctcaat
     3241 ccttttcaca agggaatatc agtaattcaa ctaatccatt ggatatggcg atcgtgggtg
     3301 gtgggttctt tcgcttaagt gataatggtg cgattagctt cagtcgcaat ggccagtttc
     3361 acctcgataa gagtggattc ctggttaatg cagataactt aaatgtgacc ggttatcctg
     3421 ctgataagaa tggaaagatc attgtgagcg gaccaacgaa tttacaactt tcaactgccg
     3481 aaatgacccc gcaggctaca acaacatttg aaacaggagt caatcttgat tcgcgcacgc
     3541 tggtaccagc tactttaccg gcttttgatg ctaccgatcc tgacacttac gctaggtcca
     3601 cttcaggaac tatctttgat agtttaggca actcacacgt gtttacctta ttttttcaaa
     3661 aatcagcgcc aagtacttgg gatgtctatg cgactgtcga tggtgcagtc gatgcttcgg
     3721 gtgtgcccgt tggtgttcaa ttggatggtg gttctccgtt aacgctgact tttgacagta
     3781 atggggttat gactgggcca accggttcag taactgcgtc agtagatttg agcgctgttg
     3841 caaccaatct aggaattgct gactggggtg ctacgacacc actcgatttt gatttcgaca
     3901 ttaccagctc tacacaattt ggtagtaatt ttgcggtaaa caaactgagt caagatggat
     3961 ttacttcagg cagactcgct ggctatggta tttctgatga tggcactatc caaggtaatt
     4021 acacgaatgg agaaaataga acactgggac aacttgttct ggctagtttt gcaaatccca
     4081 acggtttgaa gccacaagga aataatcaat gggttgaaac ggcagcttct gggttaccac
     4141 tgatcggtgt gccactaact ggaacgctcg gagagattca atcggcagca gttgaagaag
     4201 caaatgttga tttgacgaca gagcttgtca atttgataac agcgcaacgg gtttaccaag
     4261 caaatgctaa gaccatcgag acacaagatg ctgtattaca aacactcgtt aatctttaat
     4321 cagaatttat tttttatttg atttgagtga ttgaacttac aggatttagc tatggaccga
     4381 ttgatttaca ctgccatgac tggcgcaatt catacgatga ctcaacaagc aacaactgcc
     4441 cataatttag ccaatgtttc gactgccggc taccgtgctg aagttgattc attccgggca
     4501 gtacctgttt atggagatgg attgccaact agagcgtttg tattagattc aaccactggc
     4561 gctgatttta caccggggcc gattcatcgt acgggtcgtg atttggacgt ggctgtacaa
     4621 gggtcgggat ggatcgcggt tcagctagaa aacggggaag aagcctatac gcgtcacggt
     4681 aatctacaag ttagtcctaa tggattattg actacaaccc aaggtggcgc agcagttaaa
     4741 ggtgaggggg gcgccatttc acttccaccg gatagtcgtg tggcaattgg taaagatgga
     4801 acggtttcaa tgattcccaa tgatccaagg gtgaccgcag tgattgtagt cggtcgaatt
     4861 aaactcgtga atccaccgcc tgagcagctc aagaagggcc ttgatggctt attccggtta
     4921 aaagagggaa aagaagcgcc agtcgaccca aatgttgtat tagttgaagg agcgattgag
     4981 ggaagcaatg tcaatgttgt gcacgagatg gtcaatatga tttcgcttgc gaggcaattc
     5041 gatatgcaaa tgaaaatctt agatagtgca cagcaaaatt cccaacgtgc cagtgaattt
     5101 atggtttata gcggttaatt agtagcattc tgacagaaag gtaaaaaatt atgattcgtt
     5161 cattatggat atcaaaaaca gggctagacg cacagcaaac caagatggat gtcattgcta
     5221 acaacttggc aaatgttagc acgaacggct ttaaacggat gcggccagtt tttgaagact
     5281 tgctctatca aactatccgt cagcctgggg cccagtcttc tcagcaaaca cgtctgccat
     5341 caggacttca aatcggaaca ggggtacggc ctgttgcaac agaaaatatt atgcttcaag
     5401 gtaatttttc acaaacagga aatacgcggg atattgccat taaaggggaa ggtttttttc
     5461 aaatcatgtt acccgatggt acatttgctt atacgcgtga tggtgcattc cagtccgatg
     5521 atcaaggtca ggtagttaca tcgagtggct ttgttttgca gcctccggtt tcaattccac
     5581 ctaatgctac cagcattacc gttgggcgtg atggaactgt atcagccata ttgccaggcg
     5641 ctacattgtc tacccagatc ggcaatattc agcttgtaag cttcattaat tcagctggtt
     5701 tgcaaagagc aggagagaat ttatattttg aaaccgtctc cagtgggcca cctaatccca
     5761 atatccctgg tacaaatggg ctaggtttac tctatcaagg tttcgttgag acttctaatg
     5821 ttaatgtggt cgaggagttg gttgaaatga tacaaactca aagggcctat gaaatcaatt
     5881 cgagatctat acaaacttcc gatcaaatgc ttggtaggct aactcagcta taacatcatc
     5941 aatatgcgaa atcttattaa cctcatactt agaagcaata agatcagaat gttgggattg
     6001 atgataataa tcatcggggc agcgggttgt tcattgctga cacccgctcc aaaggttcat
     6061 caaccgatga cgatgcaacc tcatccggtt cccagtatca cacctagaga agcaaatggg
     6121 tcaatttatc atgttgctta cagccatcct acccagcgct atattccctt atttgaggac
     6181 cgccgtgctc gtggaattgg tgacacaatc attgtcaatc tcaatgaaag aacgaatgcc
     6241 agtaaaagat ctgcgagtaa tatcgggcga gaaagccaaa ctagcttttc tatcccatcg
     6301 tttttgggat tgccattaaa gaaaatcttt caaggcgtag atcttgaaac agagtcagaa
     6361 aataagtttg atggaaaggg tgcaagttcc agcaataaca atttcaccgg aaccattact
     6421 gttacggtgg ttgatgtttt acctaatgga aacttactgg taagcggtga aaagcaagtt
     6481 ggtattaatc aaggacatga atttattcgt ctttctggtg taattaatcc aatcaatatc
     6541 atgaataaca cggtttcttc aatccaggtt gcggacgcac gtattgaata ccggggaagt
     6601 ggttatctgg atgaagcgca aacgatgggt tggttatctc gattctttct taccgttttg
     6661 ccattctaat catgcaatag ctgattcatt tatcgattca aaacctaact aagagacatg
     6721 agtatgttga ttttccgtag atggatatta tggtttagtt taataaactg cctgattcta
     6781 ccgagctata gttttgctga acgtatcaaa gatcttgcaa gtatccaagg agtgagggcc
     6841 aatcaattga ttggctatgg cttggtcgta gggctggata atagtggcga tatgacaacc
     6901 caaactcctt ttacggtaca aagtattgtc agtatgctcg ggcaattagg tattaattta
     6961 ccgcctggaa ctaatctgat gttgcgcaat gttgctgcgg ttatggttac cgcaacttta
     7021 ccagcattct caaggccagg gcagttgatt gatgttacgg tttcatcaat gggaaatgct
     7081 aaaagtattc ggggaggaac attattgatg acccctttaa aaggaattga taatcaagtt
     7141 tatgcagtcg cacaagggaa tgtactggtg ggcggtgctg gaggttctgc ggcaggcagt
     7201 agtgttcaag taaatcattt agcatcgggt cgtatccccg gtggaggtat tgttgagcgt
     7261 gcggttgaat cagctttggg tcagggagaa ttcgtgaatc ttgagttaca ttccacagat
     7321 ttcacgactg cacgacggat taccgaagct attaaccgta tatttcctca cgggacggct
     7381 tactctgtgg atggtcgtgt tattcaactt aaggcgccgc aggatgttaa tcaacgtgtt
     7441 gcatttattt ctcaaattga aagccttgat gtgacccctg cgcaggggat agcaaaggtg
     7501 attgttaatg ctcgaaccgg ttcagttgtc atgaaccaaa ccgtaacttt agaagccagt
     7561 gcagtagcgc atggcaacct ttctgtcatc attaacagcc agccgattat tagtcaacca
     7621 ggcgcttttg ctgaacgggg agaaacggtt gttacttcac aagcacaaat tgaagttcgc
     7681 tcggacgaag ggaacctcat gatggtacag agtggtgcaa atttggccga ggttgtgcaa
     7741 gcgctaaata ccattggcgc gacaccacaa gatttgttgg ctattttgca agcgctaaag
     7801 gctgcaggct cactgcgtgc agagttagaa ataatttaat cgtaatgttc aacaaggctg
     7861 ttgcatgaag ttactaaaca catttgatac ctcaactcag ttggcaattg ataccaaggg
     7921 tatcgataac cttcggctac aagcaagaca gaacgaagac gaaggattga aacaaactgc
     7981 aaagcaattt gagtcattaa tggtcaatat gatgctaaga agtatgcgaa gcgcaacgcc
     8041 gaagaacggt atctttgata gtgaacagtc acagttttat acacaaatgc tcgatcagca
     8101 actttcacaa aatctttctt cccaagggat aggattagcg gaaatgctgg tccagcagct
     8161 taaaaaagat tcttctggga atttgattga tcatccaatc tcagaagctg agtcaatcct
     8221 atcatccatc gaatcaagtg ctggaaaaaa tgggttgatt tcattggcgc caagtgaacc
     8281 taaggatttg ccaaattcgc tgtggacaac cccgaaacag gagacgaata ggccgatatc
     8341 aatgttccaa tcatatcagt cgacagtgat gccgttggaa gtaaataaat caaatgcttc
     8401 caaatcaatg caatcattgc aaggtaagcc aaatgatttt gttaatttgg ttcagcctta
     8461 tgcgagagcc attgctaaag aaacaggtat tccagagcag tttatgattg cacaggcagc
     8521 gcttgaaact gggtggggaa aacatcaaat tcgtttgtca gaaaatcaac ctagctacaa
     8581 tctttttgga attaaagcag gaaagaactg gaaagggcct gtggctgagg ccgttacgac
     8641 tgaatatatt aatggagtag cacagaaagt agtcgataaa tttcgtgttt atcgttcata
     8701 tgaagaatcc ttccgtgatt atgccaatct tctcttaacg aattcacgtt acagtggggt
     8761 acttaagacc caaagtgcag ttgcttttgc ttgggggcta cagcaagcgg ggtatgcaac
     8821 tgatccatac tatgcgcaaa agttaattaa aatcttgaat agtgaagtat tccaaattaa
     8881 taaaattcca agttaaagag cttctaattg cttaactgat gaatattttt gccgttaaca
     8941 atactagttg aaattgattt aattgtaata tgcgataaag gaacaagata atggggaata
     9001 gtatattaaa tattggagtt tcaggacttc aagcagctca ggcagcttta ttgacgacta
     9061 gccacaatat tagcaatgcg catacaccag gttataatcg acaacaggct atacagagta
     9121 ctaatattcc tcaaccggct ggaacaggat ttcttggtca aggtgttcat atttcaacgg
     9181 tgcagcgagt ttacagtgaa tttctttata gccaaggatt gcaagcgcaa acacagagta
     9241 gtcagctaga tagttactat actcagatta agcaagtgga taatctgctt gcggatacga
     9301 cttcaggcct tacgcctgca ttgcataatt tctttaaagc agtgcacgat gtggcttcca
     9361 atccttcatc agtcccttca aggcagtccc tgttgagtaa tgcagaagct ctggttggga
     9421 gattccagag tatggatcaa cgtctatcgg aaatacgcga aggtatcaat agtcaaataa
     9481 ctagtactgt tgatgaggtt aattcattag ctaaagaaat cgctacatta aatatcaata
     9541 ttcaggctgc agaaggttca actaatgggc aaccggttaa tgatatgctt gatcagcgtg
     9601 atacattagt caggcaactg agtgagctag ttaatgtgac ggttgtacgt caagataatg
     9661 ggcatcataa tgtttttatt ggtaatggtc aggcgcttgt agtcggatcg cagtcaatgg
     9721 cccttaaagc acttccttcc cctgatgatc ctggcaaaat tactattgcg ctcgatgcag
     9781 gtaataatac tattttatta cctgaaaaac agctacaagg tggaagtttg ggtggaatgc
     9841 tagattttcg ccagaactca ttagataaag cacaaaatac actaggatta attgcaattg
     9901 gactcgccca aacttttaat gatcaacacc atttaggtca agatttaaat ggtgcgctgg
     9961 gtgaagattt ttttgaagtt cctgaaccca aagtaatatc gttagcgggg aatagttcaa
    10021 cttcaacaat aacagcgagt attagtagtg taggtgatct taatacgagt gattttcagt
    10081 tcaactacga tggatcacag tatacgttaa caaggttgtc tgataatgta tcagttagtt
    10141 cagcaacagc cccatccata gggagcccac tcacattaag tggtatcagt gttaccgctg
    10201 caactattca acccaatgaa agctttgtaa ttcaacctgc tcgtaatggt gcaaaagata
    10261 tagcaaccgc tattaatgat acatctaaaa ttgcagcagc agccccaatt cgttcgcaag
    10321 ccgcaagtac gaataccgga tcagctacga ttagtgctgg aattgtaaat ccattaccca
    10381 ttgatcctga tttatcacaa tcagtaacga ttaagtttca tacaccttct gatggtatgt
    10441 ttgatgttac tggaacagga gcgagtttgc ctgccatgaa tcaaacctac actgaaggtg
    10501 ccgatattag ttttaatggc tggactgtgc aaattagtgg agtccctgaa gctggtgatg
    10561 agtttacgat ttctccaaat acaggaggta ttgctgataa ccggaatgca ctattgttag
    10621 cgggattgca aaccaagaat acacttcaag atgggacggc ctcatttcaa tcaacttatg
    10681 cacaattggt cagtcaggtt ggtaatagaa cacgagagct ggaagtcact agtaaagcac
    10741 aggctaattt acttgctcaa acagatcaat cgatacaggc tttgtcaggt gttaaccttg
    10801 aagaagaagc agctaatttg ctacgttatc agcaagcttt ccaagcgtct gggaaagtca
    10861 ttgaggttag tcattcgttg tttgatacct tactcagaat ttaaaagaac cgattcaaat
    10921 tagaggagtt aaccgagcat gcgcgtaagt acaaatacca tgtatgatca aggagtgcaa
    10981 tcaatgctta agcaacaaga agctttattt aaactccagc aacaaatctc cgtgggcaaa
    11041 agaattgtta cgccatccga tgatccagta gcggctgcac gtgctcatga agtatcccaa
    11101 gctgcttcga ttaattctca gtatgctgag aatcgtgata aagcaaaagc cagtttgggc
    11161 ttagttgaaa gtaatcttca aggtgtctca acaattatac aaaatgttcg gtctattgct
    11221 gtggctgcgg gcaacccaat tttgtctgaa agtgatcgaa gggtaatggc tatagagcta
    11281 agaggtcgat tggaggaatt gatcggatta gctaatagtg cagatagcga aggtaatttt
    11341 ttattttctg gttatcaggc gaaagttcaa ccttttgtaa agaatgcagg tgatgttcag
    11401 tatgctggtg atcaaggaca acgtcttacc caagtcacga gttctcgtca gattgcagtg
    11461 agtgacacag gtgcaaatat ctttgaacgg gtcatggatg gtaatggggt ttttactact
    11521 tcagcaaatg ctactaatat tgggactggg attattggtg ttggttcggt aactgatcca
    11581 actaatttaa cgggcgatga ctatgaaata aattttcatg tgacccaaga tccgctcact
    11641 ctaaaagata tcattaccta tgatgtgttg aatacgacaa caagttctac aatttccacc
    11701 ggtaatactt ttgtggatgg aaatatgatt gcttttgatg gattacagtt cgatattaag
    11761 ggtaaaccag ctgatggcga tcaatttaaa gtagaaccca gttctcaaca aagtgttttt
    11821 aaaacgatcg ataatttgat taacgtttta gaaacgacaa cgaatgacca aagtggtaaa
    11881 acaaatttaa caaattcatt gaatactgct atgcagaacc ttgatcaggg tctggataat
    11941 gttttgagta tacgagcatc agttggagtg aaactgcaag agatcgatgc tttacagagt
    12001 attggagaag ataatgcctt gcaatatgat caaacactct ctcgattaca agatctggat
    12061 tatgcaaaag cgttatctga ttttacaaga caacaaggtc ttctagaagc tgcgcaacaa
    12121 tcttttattc gtatttcagg gctatcactt tttaacaggc tataaaagaa acatttaatg
    12181 ctatgtttta ttgaggtatt gctgatgcag atgtctaagc cataccaata aaacagcgct
    12241 gatgggaagc gccaataata taccaataaa accgaaaagc tgtccaaaag caagcagagc
    12301 aaagataact ataactgggt gtaagccaat acggtctccc actagccagg gtgtaaccag
    12361 catactttct aataattgac cagaaccgaa tacaactaaa acccagataa tatctcccca
    12421 atcttggaat tggaccagtg cagcaaacgt agccaatgac aaaccaacga tcgcacctaa
    12481 atagggaacg aatactaaaa ttccagcaac taggccaata ggtaaagaga actctaatcc
    12541 agtcaaccat agtccagtta catagaagat acacattaat aaaataacag ctatttgccc
    12601 cctaaggaat tcagcaagaa ttcgatctgc ttcgcgtacc attgggtaaa tctgtttttt
    12661 ccatatggat gggatccatt cgttgaactc tgcaagtaaa ctgttccaat ctcgtaataa
    12721 ataaaagagt acgacgggta ccagtagcag attcataatg aaatctaata tagccatacc
    12781 tccacttgtc agagaaggta gcattttggc tgcaatgcct ccagcgcttt tccaatgttc
    12841 agtgaacgct tgctttagta cagcaatatc taattgaaga tcaatcccaa gcgtagattt
    12901 tagccatggg attcctgttt cacgaattgt ttcaagatat tcgggtgttc gttcgattag
    12961 ccgcaaaatc tctcgctcga ataagggcat cattactagg attaatgccg taaaaatggc
    13021 aagtgataaa accatgacca gaattgttcc aatcgttcga gagatattat gagtgttcaa
    13081 ccaagaaacg aatggattac agatataagc aataactgct gctaatagaa acggtgttaa
    13141 tatcggactt agcagataag ttaaaaaacc agaaacggcg attagtagca cccaaaaaag
    13201 aaagtgacga tcaatagaat gtttggaatc catttgcggt aaaattacga atgcaaatag
    13261 tgccactcta actgtaacca attgagaact caacttgact tcatctgatt ctaagtattc
    13321 tactgctgat caactctcgt atcgtgacgc gggcgtcaat attgatgcgg gtaatttatt
    13381 agtagaaaga ataaaaccct ttgcaaaacg tacctggcgg ccagaaatta ttggagggat
    13441 tggagggttt ggcgcactat ttgaaatttc aaataaatat cgacaacctg ttttagtttc
    13501 aggaactgat ggtgtgggta ccaaactaaa attagctttc caccttaatc gccatgatcg
    13561 tatcggtatc gacttagttg caatgagtgt caatgatatt ttagtccaag gtgctgaacc
    13621 tctctttttt ctagactact tcgcttgtgg taaattgaat gtcgatatag cggcgaaagt
    13681 catagaaggt attgcagtag gctgtgagca agctaaatgt gcacttattg gtggggaaac
    13741 agcagaaatg cccggaatgt atcctgaagg ggaatatgat ttagcaggtt ttgcagtagg
    13801 cgttgtagag aaggaccaaa taattgatgg aacctcaatc gctgaaaatg atgtaattct
    13861 gggtatggcc tcaagtgggg cgcattctaa tggtttctca cttattcgta aaattcttga
    13921 ataccatcca gttgattcag attctaagct tgtagatgat gttaagctcc tagatgcaat
    13981 tatggaaccc acacgtatct atgttaaagc catactagaa ttgaaaaacc atataccaat
    14041 taaaggcctg gctcatatca cgggtggagg tcttcttgaa aatattccac gtgttcttcc
    14101 agcaaatctt agagcagtgc tgcataaagg ttcttgggcc gtaccaccat tatttacatg
    14161 gctgcaagca catggaaatg ttgctgatga agaaatgtat cgagttttta attgtggaat
    14221 aggaatgata atcgttctag caccggaata tgtcgatgaa gctttggcag tgcttaaaac
    14281 ttataatgaa accgtctggc aaatcggtac agttcaacca aatcctacaa atgatcaacg
    14341 tgtattgatt gtttgattta ggaaatgatg caatcgttgg tcattcttat ttctggtcga
    14401 ggtagtaact tgcaagcctt gatcacagca aacttacctg tcaagtcaat cgtcgttatt
    14461 agtaacaatc cttcggcatc aggactagca gttgctcagt cgcatcagat tgcaacgcat
    14521 gttatcgacc atcgatgtta tctggatcgt gattcatttg atgccgaact tgctagacaa
    14581 atcgatagat accaacctga tttaattgca ctggctggtt ttatgcgtat acttggtgat
    14641 aaattcatta atcgctatca tggtaagtta atcaatattc acccatcttt actaccgtct
    14701 tttccaggac taaacaccca tgcgcgagct ttggagcaag gtgtaaaaat tcatggttgt
    14761 accgttcact ttgtaacgaa tcaattagat catggcccga ttattattca agccgcagtt
    14821 cctgtttttc ctgatgatat gcccgacatg ttagctaatc gggtcctaaa gcaagaacat
    14881 ttggtttacc ctaccgcagt acgctggttt ttagaaagcc gtatcaaact aaaagatggt
    14941 caggtcaaga tatcatcagc cttgaatgtg gaccaaacgc tctattcacc gactattgat
    15001 tagaagttct tcccccctgg ttttcaaaga gataatgagt agtagctatc tacacaacta
    15061 gcgtgtaatt attaaattct gactttacta ttcatcatgc gcttaacaac tattcaactg
    15121 gaaatggcta ttcaagctat cagaaaaatt cttcccctca attttcctgc cgacattctt
    15181 atgcgtggat ttttccgtga gaatcctatg ctggggcata atgatcgagc cattattgca
    15241 gaaattgtgt ttggtatttt acgccataag tattttttag acacactagc tgaaaaagct
    15301 acgccgcgcg cgcttttgtt agcttattta gctaaatttc aaggtataaa ttcacgtgag
    15361 ttgataagtt ttgcaaccga gagtgaaata aaatggcttg cacatgtaaa aggtgctcag
    15421 attgcaatgc agccgcttgc gatacaagcg gagttaccgc aatggttgat cgagaagtta
    15481 caaacttacc tgccagatga agagattctt aaacttggag ttgcattgca acaacctgct
    15541 cctttagatc tgcgggttaa tacgttgctt gcaaagcgtg aggaaatact aacccagcta
    15601 gaacaagcgg aagtaaaagc aaacgcaatg ccttactcac cgattggaat tcggttagca
    15661 gaaaaaaccg cgatcaatca acatccatta tttcttgagg gaaaagtaga aattcaggat
    15721 gagagtagtc aattatctgt gtacttactt gcgccgcggc ggggagaaat gattgttgat
    15781 ttttgtgcgg gttctggcgg aaaaacgttg ctgatagggg cactcatgca gtcacatggg
    15841 cgtatttatg cattcgatgt ttcggataaa cgtcttaata atctgaaacc tagacttaaa
    15901 cgttctggtt tgtctaatat tcatcctata cgtatcgata atgagaatga tttaagagtt
    15961 aaacggttag caggaaaaat tgatagagta cttatcgatg tgccgtgtag tggtttaggc
    16021 acattacgcc ggaatccaga tttgaaatgg cgacaatctc cagaaagtat cgaagaacta
    16081 aaaattaagc aaagggctat tctaaacgta gcaagtaagc tattaaaacc gggtggaagg
    16141 atcgtttatg ctacgtgtag tttactccca gaagagaatg aagaaatcgt tgaaagtttt
    16201 ttagcaaatc atactcagtt tatagcgcta aattgcggtg agttgctggc ccaacagaaa
    16261 ataccgctta atactggaaa ttatttacaa ctgtcacccg ctaatcatca tacggacggc
    16321 ttctttgcag cagcacttga acttaagaaa aattagcttt tttaagtaaa agaacagaag
    16381 atattttaga a
//
LOCUS       DAOQLJ010000037        15913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1329_length_15913_cov_5.651343, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000037 DAOQLJ010000000
VERSION     DAOQLJ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 15913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15913
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1329_length_15913_cov_5.651343"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(491..2908)
                     /gene="lon"
                     /locus_tag="PK522_04270"
     CDS             complement(491..2908)
                     /gene="lon"
                     /locus_tag="PK522_04270"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNC40980.1"
                     /translation="MTSDAENKDSDSIVLPLLPLRDVVVFPHMVIPLFVGRAKSIKAL
                     ELAMESGKNILLVAQKSAAKDDPSLSDLYGIGCVSNLLQMLKLPDGTVKVLVEGNYRA
                     HIREYIDSENYLLGNIEEIPADNQNTPEIEAMRRALITQFDQFVKLNKKIPQEILASL
                     SSIDESGRLADTVAAYLPLKLEQKQEILEIVNTQKRLERLLNLLETELDILQVEKRIR
                     GRVKRQMEKSQRDYYLNEQVKAIQKELGEGEEGADLDEIEKKIKAASMPKEALAKAES
                     ELKKLKLMSPMSAEATVVRNYIDALVTLPWKHKSKICKDLSAAEAVLEEDHHGLEKIK
                     ERIVEYLAVQQRVSKLKAPILCLVGPPGVGKTSLGRSIARATNRKFVRMSLGGVRDEA
                     EIRGHRRTYIGSMPGKILQNLSKVGVRNPLFLLDEVDKMGMDFRGDPASALLEVLDPE
                     QNNAFVDHYIEVEYDLSDVMFVATANSLNIPPALLDRMEVIRLSGYTEDEKLNIAERY
                     LLPKQMTNHGLKEDELSISHSALCNIVRHYTREAGVRSLEREISTICRKVVKTILTQK
                     KKGKQQVVVNARNLDKYLGVTRYTYGTAESKNQVGQVTGLAWTEVGGELLTIEAAVLP
                     GKGKTITTGKLGEVMQESVQAALSVVRSRAAVLGIADEFYLKKDIHIHLPEGAIPKDG
                     PSAGIGICIAMISALTNIPVRATVAMTGEITLRGEVLPIGGLKEKLLAAHRGGIKTVL
                     IPEDNVKDLSEIPENIISKLKIKPVKWIDQVIDFALEYKPEIAAAPIPTPLSEGDENL
                     RTTVVKH"
     gene            3265..3684
                     /locus_tag="PK522_04275"
     CDS             3265..3684
                     /locus_tag="PK522_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40981.1"
                     /translation="MKKLIFAGLLSIITFHTAVNAKTEEPIQFAQLAPAKPAIEKPRT
                     TNMRENKKIMLNQEEVEKNAKQKQPSSSASGTPQADRLLGNRGYNDPTQTDNPGLGHD
                     RGFNKGKSEKDNNRFNNANPPNKIHRSNNIHMGVPAY"
     gene            3897..7358
                     /locus_tag="PK522_04280"
     CDS             3897..7358
                     /locus_tag="PK522_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HNC40982.1"
                     /translation="MQQNRLHLLSSKRFLPLFASQFLGAFNDNIFKNALIIYMTYVVI
                     DQSSVDPEIMVTLAAGIFVLPFFLFSSTAGRLADKWDKAKLIRIIKFIEIPLIIGAAA
                     AFLLHSIPLLMILLFLMGMQSTFFGPLKYGILPDQLLKNELIEGNALIEAATFLAILA
                     GTILGGLLILANNGTNLVAIVMIMTAFAGWLASLFIPSTPARSPEIKIGFNIWKDTQA
                     LLHHIQQNKIIFRSILGISWFWLLGATYLSQLPTFSKNIIGGNEQVVTLFLVIFSVGI
                     AIGSLLCNRLLKQEIAATYTPVGILGVTLFSIDLFFAAGQISPAENTALIGATEYLST
                     FTHWRILFDLLGISICGGIFIVPLYAIIQDCSETSHRSRVFAANNIINALFMVIAAIT
                     ISLMYYSNFSVTDCFLTLAILNGFVALYISTLLPHALVKSLLQALLYTCYRLEIKGLE
                     NYRDVGEKSIIVANHLSFLDAILLSVCIPDQLCFAINTHIAQKWWIRPFLFLAETVAI
                     DPTNPMSTRLLIDRVKKGKRIVIFPEGRLTVTGSLMKIYEGPAMIADKSGATLLPISI
                     VGAQYTPFSRLKGKLRIRWFPRITITIMPPEWLNLPNEVRGKQRRQLAGNKLYDIMTS
                     ALFHSNDLQQTLFQSLLDASHIHGRNHIIAEDIERQPITYSQLLTRSFILGSRLCKFS
                     KPAENIGLLLPNSIGAIVTFFALQAYARIPAMLNFSVSKANLMNACQIASIRTLVTSS
                     RFIEVAKLTSVIDNFRAMGITVLYLEDLRQEIHWWHKLLGFLAGLIPQTYYRLCCPAS
                     YPDTPAVILFTSGSEGLPKGVALSHVNLQANRFQVAARIDFGPTDIVFNVLPIFHSFG
                     LTGGTLLPILSGIKAFLYPSPLHYRAIPELIYDSNATILFGTDTFLTGYARHANPYDF
                     YSLRYVFAGAEKLRESTRRIWSENYGVRIFEGYGATETSPILSLNTPMHNRPGSVGRM
                     LPGIAYRLEKISGIEEGGKLLVSGPNIMMGYYLINAAGQINPPNQGWYDTGDIVAIDQ
                     DGYIFIKGRAKRFAKIAGEMISLTAVEAYISKLWPNYQHAVVAIPDSKKGEQLVLITT
                     NPTAGRNEILTYVNQQGFGELSIPKTILIKEDIAVLGSGKTDYVALLDWVNTQSTQTH
                     NKDQLND"
     gene            complement(7355..8758)
                     /gene="der"
                     /locus_tag="PK522_04285"
     CDS             complement(7355..8758)
                     /gene="der"
                     /locus_tag="PK522_04285"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNC40983.1"
                     /translation="MNPTLVLVGRPNVGKSTLFNRLTRGRDAIVADLPGLTRDRHYGQ
                     GKVGKKPFIVVDTGGFEPVVKSGILYAMAKQTLQAIDEADVILFVVDGRQGLTPHDKI
                     IAEQLRKSGQRIQLVVNKTEGMNTSLITSEFHELGLGDPCAVSATHGQYIAELVESAL
                     APFEFLAEEEKITLEAGPKIAIVGRPNVGKSTLINTLLGEERVIAFDQPGTTRDSIYI
                     DFQYHQRPYTLIDTAGLRRRGKVFEAVEKFSVIKTLQAIEDANVVVLVLDAQNDISDQ
                     DAHIAGFVLETGRALVVAINKWDGLDSYQRETIKREILRRFIFLDFAKFHFISALQAK
                     GIHELFLSIDTAYKAAMASLPTPKLTRVLHAAVMKQSPPRGGMSYPKLRYAHQGGSNP
                     PLIIVHGSMLNHVPETYRRYLENTFRDFFNLEGTPLRVEFRVGHNPYANKKPAPLTEE
                     EAKRAHSRRRRNRKKYG"
     gene            complement(8849..10102)
                     /gene="bamB"
                     /locus_tag="PK522_04290"
     CDS             complement(8849..10102)
                     /gene="bamB"
                     /locus_tag="PK522_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HNC40984.1"
                     /translation="MISYTLVFFLVFEIKKIRQKFFSYFGLVLLTGCSSFGGLGGFGD
                     FGDFGISNLIDRFSSGKDEIQIDEEEIAALKALPTLPLIWKSKVSESEIAVFEPIYEN
                     GAIYTADEKGFLTRLDPLNGKETWQIKTKLKLSGGVGAGEGLVLVGTFNGEVHAFDES
                     GGALWKARLTSEVLSAPQVHRGIVVVRTGDGRVFGLDAADGSRKWVYQGVTPALTVRN
                     FAGALVARGAVFVGLSGGKLIAMNMLNGNIGWEAAVSQPKGVTELERMTDITSLPVID
                     DQQVCAVAYRGRIACFEIMDGNQIWARDASSHVGLVMDNNYIYVSEERGAVVAYDKRS
                     GAMMWKQAKLGTRKLSAPQLVGGSSLLVVADAEGNVTLLNRYDGNLVARATTDESPIY
                     SRPEYIPDGFIVQTSKGGIYAFLPQ"
     gene            complement(10106..10750)
                     /locus_tag="PK522_04295"
     CDS             complement(10106..10750)
                     /locus_tag="PK522_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC40985.1"
                     /translation="MVAYNPEEQEKIEGLKAWWSTHGSSVIIILSTMIAVMAGMQAWK
                     YYHKQQALQAADLFAVLQQQIDKGGSSEKINDALHLLTTGYPESGYASRAALIAAQAN
                     KNLGNLPEAKAKLQWILDHAKELEIKDIARLRLAGVLLDEKKFDDALKLLDSQHGESF
                     SGLYADLKGDILVAAGKPSEASAAYQKAYDRFSMSKSNYMNLVQMKLDAVKVSK"
     gene            complement(10773..12035)
                     /gene="hisS"
                     /locus_tag="PK522_04300"
     CDS             complement(10773..12035)
                     /gene="hisS"
                     /locus_tag="PK522_04300"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNC40986.1"
                     /translation="MSSTIRAIRGMNDILPDEVYQWDFFETTIRDWLAAYGYRNIRTP
                     IVEQTDLFVRSIGTATDIVEKEMYTFVDHLNGESLTLRPEGTASCVRAVLEHSLLYTG
                     PQRLYYYGPMFRHERPQKGRYRQFHQVGVEAIGYANAEIDAELIIMCARLWRQLGISN
                     LRLEIGTLGSVASRAVYRDRLIGYFEQHLQHLDEDARRRLYTNPLRILDSKNPAMCEL
                     ILNAPRLLDDLDEESLAHFKELQDRLQQQGIDFEINLKLVRGLDYYNRTVFEWITDKL
                     GAQGTVCAGGRYDGLIEQMGGKHTPACGFAMGIERILALMNETGVKIPQSEVDAYVVY
                     QGEMAAKFCWKVAEYFRDNGLKVILHCGGGNFKSQMKKADASGARFAIIIGDDEANDS
                     EVSIKPLREAVEQVRVGLNEAVALLKQH"
     gene            complement(12028..13251)
                     /gene="ispG"
                     /locus_tag="PK522_04305"
                     /gene_synonym="gcpE"
     CDS             complement(12028..13251)
                     /gene="ispG"
                     /locus_tag="PK522_04305"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNC40987.1"
                     /translation="MLRRKSVGVRVGSTVIGGDAPVVVQSMTNTDTADEVATAIQVAQ
                     LARAGSELVRITVNTTEAASAVASIRSRLDDMGCHVPLIGDFHFNGHKLLTAYPACAK
                     ALAKYRINPGNVGHGKKRDEQFATLIETACHYDKPVRIGVNWGSLDPEMLARIMDENA
                     KSSDPLDAGQVMRKALITSALESAAKAEELGLPRDHIILSCKVSGVQDLIAVYRDLAA
                     QCDYALHLGLTEAGMGSKGIVASTAALSVLLFEGIGDTIRISLTPEPGGDRSREVIVA
                     QEILQTMGLRAFVPLVAACPGCGRTTSTYFQELAKSIQTYVREQMITWRDEYDGVENM
                     TLAVMGCVVNGPGESKHANIGISLPGSGENPVAPVYVDGQKTVTLKGDNIAGEFRQII
                     DDYVRTKYQRKVANV"
     gene            complement(13301..14296)
                     /locus_tag="PK522_04310"
     CDS             complement(13301..14296)
                     /locus_tag="PK522_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNC40988.1"
                     /translation="MNENNGDNEMIASNGESMMSRSEEMDNLNNRALPESIGQILRDE
                     RIKRNLSIGEVSRRLRLSEQKIAAIEENDFSKLPSGTFLRGFIRNYANFLQLDFNSLI
                     PLLSQSAGVIVQQNSNRQSKTIPLESRRLGYSGSAYRLFISAFIVLSIMLLGYGIYNY
                     GDWGEYFVVKNGGDTSNTQADSLKQENGQTTMELSLPLPNLPSTSWNTVESFKPINEK
                     PVTTESSSLPTPVTTEIKETAQTNVLESSTDSGEKTVQLTFSRESWVEIRDSKKKVIF
                     MKTNARGSEQVVKGTPPLYLVIGNASGVGLTYNGKLVDLAPYTRKADDVARFSLE"
     gene            complement(14289..15080)
                     /gene="pilW"
                     /locus_tag="PK522_04315"
     CDS             complement(14289..15080)
                     /gene="pilW"
                     /locus_tag="PK522_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HNC40989.1"
                     /translation="MFKRLTALLISLSLAWLTGCVSPPDPESNLTPEEKRKRVQDRAK
                     IHTELAGQYYSRGQYRVAIEEAEIALRANNNYAPAYNVLGLIYMDLREDGRAQVNFER
                     AIRIAPNDADVHNNYGWFLCRNPAQIDLAIDHFMQAIKDPFYEIPEKSYTNAGLCVLK
                     RNDFEQAKAYLQKALVIHPDYPLAVLGLIQIDYLSRNLRVAQTNITRYIQSYPLSADS
                     LWLAIRIEHALGNDQAVASYGAQLQRRFPDSNEARSLREGKLRDE"
     gene            complement(15073..>15913)
                     /locus_tag="PK522_04320"
     CDS             complement(15073..>15913)
                     /locus_tag="PK522_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNC40990.1"
                     /translation="SQVGCALNCSFCATGKQGFNRNLTVAEIIGQLWWANKTLKSQRQ
                     PASMQTDHRPDEEVVGFTEQKPASDRVITNVVLMGMGEPLANFDNVVTALDLMLDDSA
                     YGLSRRRVTVSTSGIVPAMDRLRERCPVALAVSLHAPNDELRNELVPINKKYPLHELL
                     AACKRYLQASPRDFITFEYVMLDQVNDTVQHARQLVELVKDTPCKFNLIPFNSFSDSG
                     YKRSTKEAIHSFRDVLMQAGLVTTVRKTRGDDIAAACGQLAGNVLDKTRRTQRGAEGM
                     ASV"
BASE COUNT         4476 a         3867 c         2949 g         4621 t
ORIGIN      
        1 aaaaatatcg cgtaaaactg ctctaattca tgacccatca actttagtcc catttctcca
       61 tttttgcgta ggctagcatc taagccgtta actcgattga tgaaataaaa actgaatcac
      121 atgacaattt caatttaagt ttctgtacgc tgacgctcaa ggcgtcaaac gctatacaaa
      181 tagagtgtgt taacgtaggg gggaaatgaa aatcaccctt ggcacgagta accagttttt
      241 tgtgccaggt gaaaattttt taggaaaaaa cttgaaacaa caaaatgaca gtctggcttt
      301 tcctagaaaa agtcaggtct taagatgaat aagcaatcca ccatcggttt actaccggga
      361 ttacaagaaa acaggaggcg taccctattt aacttcctgc ttaaacgctt tgttaaatag
      421 ggtaattgtt atcgagaacg atacgattga atttgtgttg cttttatgcg caacaccgtt
      481 agcttcacga ctaatgctta accactgtcg ttcttaaatt ttcatcgccc tcggacaggg
      541 gtgttgggat cggtgcagca gcgatttcag gcttatattc caaagcaaaa tcaatgacct
      601 gatctatcca cttaaccggt ttaattttta acttactgat aatattttct ggtatctcac
      661 tcagatcctt aacattatct tctggaatca gcacggtttt aattccacct cgatgagccg
      721 caagcaattt ctctttgagc cccccaatgg gtagcacttc gccacgcaat gtgatttcac
      781 ccgtcatcgc cacagtcgca cggaccggaa tattggttaa cgcagaaatc atcgcaatac
      841 aaataccaat cccggcactt ggaccatctt tggggatagc accctctggt aaatgaatat
      901 ggatatcctt tttcaaataa aactcatcag caatgcccaa aacagcagca cggctacgaa
      961 cgactgacaa agcagcttgc accgattctt gcattacttc acccagttta ccggtcgtaa
     1021 tggtttttcc ttttccaggt agaaccgcgg cttcaatcgt tagcaattct cctccgactt
     1081 cagtccacgc tagccctgtg acttgcccta cctgattttt actttcagcg gttccatagg
     1141 tatagcgcgt tacacctaaa tacttatcga gattacgagc attaaccacg acttgctgtt
     1201 tgcctttttt cttctgcgtc aggatcgttt tcacaacctt acgacaaatc gttgaaattt
     1261 ctctttctag cgatctaacc cctgcttctc gcgtgtaatg tcttacgatg ttgcataacg
     1321 ccgaatgaga aatagacaac tcatcttctt ttaaaccgtg gttcgtcatt tgtttaggca
     1381 gcaaatagcg ctcggcaata ttcagcttct catcttcggt atagccagaa agtcgaatca
     1441 cttccatccg atccagtaac gcaggcggaa tattaagcga atttgccgtt gcaacgaaca
     1501 ttacatccga taaatcatac tcaacttcaa tatagtgatc gacaaacgcg ttattttgct
     1561 caggatcaag tacttcaagc aatgcagaag ccgggtcacc gcggaaatcc atccccatct
     1621 tgtcaacttc atctagcaga aacaatggat tccgaacacc gaccttactg agattctgca
     1681 atatcttccc gggcatagag ccaatgtaag tgcggcgatg gccacgaatt tcggcctcat
     1741 cacgcacacc gcctaaagac attctgacaa atttccgatt ggttgcccgc gcaatggatc
     1801 gacctagcga agttttacca acaccggggg ggccaaccag acaaagaatc ggcgctttta
     1861 gcttacttac tcgttgttgt accgccagat actcaacgat tctttccttg attttttcta
     1921 acccgtgatg gtcttcttca agcactgctt cggcagcact caaatctttg caaatcttac
     1981 ttttgtgttt ccatggcaaa gtcaccaaag cgtcaatata atttcgcaca accgtggctt
     2041 ctgcagacat cggagacatc agtttcaatt tcttcaattc ggattctgct ttagcaaggg
     2101 cttctttagg catcgatgct gctttaatct ttttctcaat ttcatcgaga tcagcgccct
     2161 cttcaccctc accgagttct ttttgtatcg cttttacctg ttcgttcaga taataatctc
     2221 tttggctttt ctccatctgc cgcttaaccc ggccacgaat ccgcttctcg acttgcaata
     2281 tatctaattc agtttctaac aagttgagta agcgctcaag ccgtttctgg gtattaacga
     2341 tttcaaggat ttcttgcttc tgttcaagtt tcaaaggcag ataagctgca acggtatccg
     2401 ctaatcttcc tgattcgtca atgctcgaca gagacgctaa aatctcttga ggaatttttt
     2461 tatttaattt tacaaactgg tcaaattgcg taatcaatgc acgacgcatt gcctcgattt
     2521 ctggggtatt ctgattatcc gcaggtatct cttcgatatt gccaagtagg taattttccg
     2581 aatcaatata ttcacgaata tgcgcacgat aattaccttc aaccagcact ttgacggtac
     2641 catccggtaa ctttagcatc tgcaaaagat tagacacaca tccgatccca tataagtcac
     2701 tgagactcgg gtcatcttta gcggctgatt tttgtgctac cagcaaaata tttttgcctg
     2761 actccattgc taattcaagt gccttgatag acttagctcg accaacgaaa agtggtatta
     2821 ccatatgcgg aaatacgacc acatcgcgta acggtagcaa aggcaacacg atcgaatctg
     2881 aatctttatt ttctgcgtct gaagtcatat ttatttataa atcacatatt aaattgtagt
     2941 taaagcatac tagactattg tttatatcac ttcaatctat tgcaacaaac ttgtatctcc
     3001 aattgcgagc tagatcaatt aattatttaa cccaatggtg ctaactaaca cgaccacatt
     3061 taattagacc accgaatgga tattcgacta tcgaaaacag gtgcacaaca caaattcact
     3121 ttattaatac tccctatttt tgtttttaca aatcgaattt acatggtaat ttatgggctt
     3181 atttgtagtt ttcaagattt gaaatatctt ttatcgtttt caagttctcg cttaattttt
     3241 ctaaccttat tagacaaaaa tacgatgaaa aaattaatat ttgcaggtct tcttagcatt
     3301 attacctttc ataccgctgt caatgcaaaa acagaagaac ccatccaatt cgctcaactt
     3361 gctcctgcta aaccggcaat agaaaagcca agaacgacta atatgcgtga gaataaaaag
     3421 atcatgctca atcaagagga agtagaaaaa aatgcaaagc agaaacaacc atcttccagc
     3481 gcgtcgggta cgcctcaagc agatcgatta ctcggcaatc gaggttataa cgatcccact
     3541 cagacggata accctggatt aggtcacgat cgaggtttca ataaaggaaa atctgagaaa
     3601 gacaacaatc gttttaataa cgccaatccc cccaataaga tccatcgcag taacaatatt
     3661 catatgggtg ttccagctta ttagaaaatc acccatcgca ttctaatctt caacgattac
     3721 cctccaataa ctcttcatcg atcgcggtta cgttctatct ggcgctaacc gccccatcta
     3781 tctaataata atctttatta ccgggtattc tttttttaaa acaatatcgt tccaatggat
     3841 aattgaaaaa gaaaaaaatc gactcacctc aaattttggg ctatttgttt aaacctatgc
     3901 agcaaaatcg attgcactta ctttcttcta agcgattctt accacttttc gcatcacaat
     3961 ttttaggcgc attcaatgac aatattttta agaatgcact gattatttac atgacctacg
     4021 ttgtgatcga tcaatccagc gtagacccag aaatcatggt tacgcttgct gcgggcattt
     4081 ttgtgttgcc attcttcctt ttttcgtcaa ccgcaggacg acttgcagac aagtgggata
     4141 aagccaagct tattcggatc attaagttca tcgaaattcc actgatcatt ggcgcagccg
     4201 ccgctttcct acttcatagc attccgttat tgatgatttt gcttttctta atgggaatgc
     4261 aatcgacttt ctttggccca ctgaaatatg gaatcttacc ggatcaatta ctcaaaaatg
     4321 aactaatcga gggaaatgca ctgatcgagg cggctacttt tttagctatc ttagcaggca
     4381 caatattggg tggcttactt attcttgcca ataacggcac caatttggta gccatcgtca
     4441 tgatcatgac agcgtttgct ggttggttag ccagtttatt tattccgtca accccagcca
     4501 gaagtccaga aattaaaatt ggattcaata tttggaaaga tactcaagcg ctccttcatc
     4561 acattcagca aaataagatt atctttcgct ccattctagg tatttcctgg ttctggttac
     4621 tgggcgcaac ctatctttca cagctcccaa cattttctaa aaatatcatt gggggcaatg
     4681 agcaagtcgt caccttattc ttagtcatat tttcggttgg gatagcgatt ggctctttgc
     4741 tgtgtaatag attacttaaa caggagattg cagccaccta tacaccggtg ggaattttag
     4801 gcgtcacgtt attctccatc gatctatttt ttgcggctgg gcaaattagc cctgcggaga
     4861 ataccgcatt aattggcgca actgagtatt tgagtacctt tacacattgg cgtatcttat
     4921 ttgacctact cggaatctcc atatgcggtg ggatttttat cgttccgtta tacgcgatca
     4981 tccaagactg ctcagaaacg tcccatcgct cccgcgtatt tgctgctaac aatattatca
     5041 atgcgctgtt catggtaatc gcagccatca cgatcagttt gatgtattac agcaactttt
     5101 cagtgacgga ttgctttctc acactcgcaa tactaaacgg cttcgtagcg ctttatatct
     5161 cgacgctttt gcctcacgca ttagttaaat cattgctgca agctctttta tacacatgtt
     5221 accgacttga aatcaaaggg ttagaaaatt atcgtgacgt aggtgaaaaa tccatcatcg
     5281 ttgccaatca tttatcgttt ttagatgcga ttcttttaag cgtttgtatc cccgatcaac
     5341 tttgttttgc catcaatacc catattgcgc agaaatggtg gataagacct tttctgtttt
     5401 tggctgaaac cgtagcaatt gatccgacca acccaatgtc gacccgatta ttgatcgacc
     5461 gggtcaaaaa aggaaaacgc atcgtgattt ttcctgaagg cagattaacc gtcaccggtt
     5521 ctctcatgaa gatttatgaa gggccggcaa tgattgccga caaatccggt gcaacgttac
     5581 tgccgattag tattgtcggc gctcaatata cccctttttc aagactcaaa ggcaaattaa
     5641 gaattcgctg gtttcctaga atcacgataa cgatcatgcc gcctgagtgg cttaatttac
     5701 cgaacgaagt tcgtggaaag caacgtcgac aacttgcagg aaacaagctg tacgatatca
     5761 tgacgtccgc tttgtttcat agtaatgatc tccaacaaac tttgttccaa tccctactgg
     5821 atgcctctca tatccatgga agaaaccata tcattgctga agatatcgag cgacaaccca
     5881 ttacctattc tcaattatta acccgtagct ttattttggg tagtcggtta tgcaaatttt
     5941 ccaaacctgc agaaaatatt ggactattgc tccctaattc cattggtgca attgtcacct
     6001 tctttgcact gcaagcgtat gcacgaattc ctgcaatgct taacttttct gtcagcaaag
     6061 ctaacttaat gaatgcttgc caaattgcaa gcattcgaac gcttgttacc tcgagtcgct
     6121 ttattgaagt tgcaaagctt accagcgtaa tcgataactt ccgagcaatg ggaataacgg
     6181 tactttattt agaagatctt cggcaagaaa ttcattggtg gcacaagctc ttggggtttc
     6241 tggcgggttt gatcccacaa acttactacc gattatgttg tcccgctagc taccctgaca
     6301 ctcctgctgt aattctcttc acatcaggct cagaaggttt gcccaaggga gtcgcactga
     6361 gtcacgttaa tcttcaagcg aatcgatttc aggttgcagc gcgcatcgac tttgggccga
     6421 cggatattgt tttcaatgtc ctaccgattt ttcactcttt tggtttaacc ggcggcacgc
     6481 ttttacccat cctgtcaggt atcaaagctt ttctttatcc ttcgccgctt cattaccggg
     6541 cgatccctga attaatctac gattccaatg caacgattct ctttggaacg gatacgtttc
     6601 tcactggcta tgctcgccat gccaatccgt atgatttcta tagtcttcga tatgtctttg
     6661 caggcgccga aaaattacga gaaagtacac gcagaatatg gtctgaaaat tatggcgtgc
     6721 gtatcttcga aggctatggc gctactgaaa cttcacccat cctcagcttg aatacaccga
     6781 tgcataatcg accaggatcg gttgggcgta tgcttcctgg catagcctat cgactagaaa
     6841 aaataagcgg gatcgaagaa ggaggaaaat tattggtgtc gggccctaat atcatgatgg
     6901 gctattattt aataaatgct gcgggccaaa tcaatccgcc taatcaaggt tggtacgata
     6961 ccggtgatat cgtagcaatc gatcaagacg gctatatttt tatcaagggg cgagcaaaac
     7021 gctttgcaaa aatagcaggg gagatgatat cgctaaccgc agtcgaagct tacatcagca
     7081 aactttggcc aaattatcag catgccgtcg tcgctattcc tgattcaaag aaaggggaac
     7141 aactcgtact catcaccact aacccaactg cgggtcgtaa tgaaattctc acttatgtga
     7201 atcaacaagg gtttggtgag ctgagtattc ccaaaaccat cctcatcaaa gaagatatcg
     7261 ctgttttagg ttcgggaaaa acagattatg ttgcattatt agactgggtc aatacacaat
     7321 cgacccaaac tcataacaaa gaccaactca atgactagcc gtatttcttc cggtttctgc
     7381 ggcgtcgact atgcgcgcgc ttggcctctt cttccgtcaa cggtgcaggt tttttgtttg
     7441 cataaggatt atgcccaaca cgaaattcaa ctcgcagcgg tgttccttcg aggttaaaga
     7501 aatcacggaa ggtgttttca aggtaacgac ggtaggtttc tgggacatga ttcagcatac
     7561 tgccatgaac aataatcaag ggtggatttg atcccccttg atgggcatac cgtaacttgg
     7621 gatacgacat ccctcctctg ggtggcgatt gtttcataac ggctgcatgc aaaactcgtg
     7681 tcaatttagg cgtcggcaaa ctcgccatgg ctgctttata agcagtatca atcgacaaaa
     7741 acagctcatg aatcccttta gcctgtaatg cagagataaa atgaaatttg gcaaaatcaa
     7801 gaaaaataaa gcgtctcaat atctctcgct tgattgtttc gcgctggtag ctatccaacc
     7861 catcccattt gttaatagcc acaactaatg ctctgcccgt ttctagtaca aaaccggcaa
     7921 tatgcgcatc ttgatctgaa atatcatttt gagcgtctaa taccagcact actacatttg
     7981 catcttcaat cgcttgtagc gttttgatta ctgaaaactt ctcaaccgcc tcaaaaactt
     8041 tgccgcgtcg ccttaatcct gcagtatcaa tcagcgtata gggacgctgg tgatattgaa
     8101 aatcaatata gatactatcg cgggtggttc ccggttgatc aaacgcaatg acgcgctctt
     8161 cgccaagcaa ggtattgatt aaagtagatt taccaacatt gggtctgccc acaatagcga
     8221 tctttggccc cgcttccagc gttatttttt cttcttctgc caaaaattca aagggagcca
     8281 aggctgattc cacaagctca gcgatatatt ggccatgtgt cgctgaaaca gcacaaggat
     8341 cacccaaacc caattcgtgg aattcagaag taatgagcga agtattcatt ccttctgttt
     8401 tattgacgac taattgaatt ctttgcccag atttgcgtaa ctgttctgca ataattttgt
     8461 catggggtgt taagccttgc ctaccatcca ccacgaatag aataacatct gcctcatcaa
     8521 tggcttgcaa ggtttgcttc gccatcgcat ataaaatacc ggatttcaca acaggctcaa
     8581 atccgcccgt atccacgaca atgaatggct ttttgcctac tttaccctga ccataatgcc
     8641 gatcacgagt taatccaggt aaatcagcaa cgatggcatc acgaccacgg gttaaccgat
     8701 taaataacgt agatttccct acattaggtc ggccaacaag cactaaagtg ggattcattg
     8761 cttagcgtaa aggctatagg agaattgttg cgtgaaagaa atgactgatt attaattaat
     8821 aacgaatcaa ctcgttttac tgccatgctt attggggtaa gaaagcatag ataccccctt
     8881 ttgatgtttg tacaataaac ccgtcaggaa tatattctgg tcgactataa atcggacttt
     8941 catcggtagt tgctctcgcg accaaattac cgtcatagcg attcaatagg gttacattgc
     9001 cttccgcatc tgcgacgact aacaaagaac tgccgccaac caattgtggt gcagaaagtt
     9061 tgcgagttcc taattttgcc tgcttccaca tcattgcgcc gcttctctta tcataagcca
     9121 ccacggcacc ccgttcttca ctgacataaa tataattatt atccataacg agtccaacat
     9181 gactcgatgc atcccgtgcc catatttggt taccatccat gatttcaaaa caagcgatac
     9241 ggccacgata agcaaccgca caaacttgtt ggtcatcaat aacggggaga ctggttatat
     9301 cggtcattcg ttccagctca gttactcctt tcggctgtga aacagcagct tcccagccaa
     9361 tatttccgtt taacatattc attgcaataa gctttccacc agatagccca acaaaaacgg
     9421 caccgcgtgc aacgagcgca cccgcaaaat tcctaaccgt caatgcaggt gtaacccctt
     9481 gataaaccca tttgcgcgat ccatccgcag catctagtcc aaaaacacgg ccatcgcctg
     9541 ttcgaacaac gacaatgcca cgatgcactt ggggtgcgct taacacttca ctggttaaac
     9601 gcgctttcca taatgcaccg cccgattcat caaaagcgtg aacttcccca ttaaatgtcc
     9661 ccaccaagac taaaccttct cccgcgccga ctccacctga taatttaagt tttgtcttta
     9721 tctgccaagt ttcctttcca ttcagtgggt caagccgtgt taaaaatcct ttttcatcgg
     9781 ccgtgtagat agctccattt tcatagatgg gttcaaatac agcaatttcg ctctcactga
     9841 ctttagattt ccaaattaaa ggcagcgtcg gcaacgcttt taacgctgcg atttcttctt
     9901 catcaatttg aatttcatct ttgccactac tgaagcgatc gattagatta ctgataccaa
     9961 agtccccaaa atcgccgaat ccacccaatc cgccaaagct ggagcacccc gttaatagta
    10021 ctaacccaaa gtatgaaaaa aacttttgtc gtattttctt tatttcaaaa acaagaaaaa
    10081 aaactaacgt gtaactaatc acctgctatt tgctcacttt aactgcgtct aatttcattt
    10141 gaacgagatt catgtaatta cttttactca tactaaagcg gtcataggct ttctgatagg
    10201 cggcactcgc ttcactcggc tttcctgcag caaccaatat atcccctttc aaatcagcgt
    10261 aaagtccgct gaaagattct ccatgctgac tatccaataa tttcaaagca tcatcaaatt
    10321 tcttctcatc cagcaaaacc ccagctaggc gcagccttgc gatatccttt atttccagct
    10381 cttttgcatg atcaagaatc cattgcaatt tagctttagc ctctggaaga tttcccaaat
    10441 ttttattggc ttgagcagct atcaatgctg cacgagaagc gtagccactc tcgggataac
    10501 cagtagtcag gagatgcagc gcatcattga ttttttctga gctaccgccc ttatcaatct
    10561 gctgttgcaa tactgcaaac aagtcggcag cttgcaacgc ttgctgctta tggtaatact
    10621 tccacgcttg cattccagcc ataactgcga tcattgtcga caaaatgatg atgacggagc
    10681 taccatgagt cgaccaccac gctttcaatc cttctatttt ttcctgttct tccggattat
    10741 atgccacgat ttcttcctag tattttaaaa aattaatgtt gtttaagtaa cgcaaccgct
    10801 tcatttaaac caactcgtac ttgttcaact gcttctcgca acggtttaat acttacctcg
    10861 ctatcgtttg cttcatcgtc accgataatg atcgcgaaac gggctccact ggcatccgct
    10921 tttttcatct gtgatttaaa atttccgccc ccacaatgca aaataacctt gagcccatta
    10981 tctcgaaaat attcagcgac tttccaacaa aattttgcag ccatttcacc ttgataaact
    11041 acataagcat ctacttcaga ttgaggaatt tttacgccgg tttcattcat caacgccagg
    11101 atccgttcaa ttcccattgc aaaaccacag gctggggtat gcttcccccc catctgttca
    11161 atcaatccat cgtatcgccc accggcacaa accgttcctt gtgcacctaa tttgtcggtt
    11221 atccactcaa ataccgtacg attgtagtaa tctaaccctc tcaccaattt gaggttgatc
    11281 tcaaaatcaa taccctgctg ctgtaatcga tcctgcagct ctttaaaatg agccagtgat
    11341 tcctcatcta aatcatcgag tagcctaggc gcattaagaa taagctcaca catggctgga
    11401 ttcttactat caagaattcg caacggattg gtatataacc gtcgtcgagc atcttcatct
    11461 aaatgctgta aatgctgttc gaaataaccg ataagccgat cacgataaac cgcacgggag
    11521 gctacgctgc ctaaagtacc aatctcaagt cgtaaattgg aaatacctaa ttgacgccat
    11581 aatcgagcac acataatgat aagctccgca tcaatctctg cattagcgta accgattgct
    11641 tcaacaccca cttgatgaaa ttggcgataa cgccctttct ggggacgctc atgccgaaac
    11701 atcggcccat agtaatataa tcgctgtggt cctgtgtaaa gcaaactatg ttcaagcact
    11761 gcacggacac aagatgccgt accttcaggg cgtaatgtta ggctctctcc atttaaatga
    11821 tcaacgaaag tgtacatttc tttctcaacg atatcggtcg ccgttccaat agagcgcaca
    11881 aacaaatcgg tttgctcgac gattggcgtc cgaatattgc gataaccata agcagccaac
    11941 cagtctcgaa tcgttgtttc aaaaaaatcc cactgatata cctcatcggg taggatgtca
    12001 ttcatccccc gaatggcacg gatcgtacta gacattcgct acttttcttt gatatttagt
    12061 acgtacataa tcgtcgataa tttgacgaaa ctcccctgcg atattatcac cttttagtgt
    12121 cactgttttc tggccatcga catagaccgg cgctaccgga ttttcccctg agcccggaag
    12181 gcttattcca atattggcat gcttactttc accgggacca ttcaccacac aacccatcac
    12241 agcgagtgtc atattctcaa caccatcata ctcatctcgc catgtaatca tctgttctcg
    12301 gacgtaagtt tggatactct ttgccaattc ttgaaaataa gtactggtcg ttcttccaca
    12361 tcccggacaa gcggcaacta gtggaacaaa tgcgcgcaat cccattgttt gcaagatctc
    12421 ttgcgccaca atcacttcac gcgagcggtc ccctcccggt tctggcgtca aagaaattct
    12481 gatcgtgtca ccaatacctt caaacaataa tacagataat gcggctgtag acgcgactat
    12541 gccttttgaa cccattcccg cttctgttaa tcctaaatgc aatgcataat cacattgagc
    12601 agccaaatct cggtaaactg caattaaatc ctgcacgcca cttactttgc aagacagtat
    12661 gatgtgatca cgtggcaaac cgagttcctc agctttagct gcactttcca gcgcagaagt
    12721 aatcagtgcc ttacgcatga cttgacctgc atccaatgga tcactagatt ttgcgttctc
    12781 atccatgata cgagcgagca tttcagggtc cagactaccc caattaacac cgattcggac
    12841 aggtttgtca taatggcaag cagtctcaat cagggtagca aattgctcgt cacgtttttt
    12901 accgtggcct acgttaccgg gattaatgcg gtactttgct aaggccttag cgcaagcagg
    12961 ataagcggtt agtaatttat ggccattaaa atgaaagtcc ccgatcagtg gaacatgaca
    13021 tcccatgtca tctaatcgtg aacgtatgct cgctacagcg cttgcggcct cagtcgtatt
    13081 gacagtaatt cgcaccagtt cagaacccgc tctcgccaat tgcgcaactt gaatcgcggt
    13141 tgcaacttca tctgcagtat ccgtattggt catcgattga acgacaacgg gcgcatcccc
    13201 tccaatcact gtagaaccta cacgaacacc gacacttttc cttcttaaca taacattatg
    13261 attttatttt attagggata attacacatt tgacaggcgt tcactcgagt gaaaagcgtg
    13321 ctacatcatc agctttccga gtataaggtg ccaaatcaac tagctttcca ttataagtaa
    13381 gtccaacccc cgacgcatta ccaataacca aataaagcgg gggcgttcct ttaacgactt
    13441 gttcagaccc tcttgcgtta gttttcataa aaatgacctt cttcttacta tctcttattt
    13501 ctacccaaga ctctctagaa aaggtaagct gcactgtttt ttcaccacta tcagttgaac
    13561 tttctaacac attagtctga gcggtttctt tgatttcagt agtaactggc gtaggtaatg
    13621 aggatgattc cgtcgttaca ggtttctcat tgataggttt gaatgactcc accgtattcc
    13681 aagaagttga tggaagatta ggcaatggca aggatagctc catcgtagtt tgaccattct
    13741 cttgcttcaa ggaatctgct tgggtgtttg aagtatcacc cccattcttc acaacaaaat
    13801 actcacccca atcaccatag ttataaatac cgtaacccag taacataatc gataaaacaa
    13861 tgaaagcgga tataaacaag cgatatgcac tgccggaata ccctagacgt cttgattcga
    13921 gaggaatggt tttagactgc ctgttcgagt tttgttgaac gattaccccg gctgattgcg
    13981 ataataaagg aataagtgaa ttaaaatcga gttgaagaaa attggcataa ttacggataa
    14041 agccacgtaa aaaagtgcca gaaggtaatt tactaaaatc attttcctcg attgcagcaa
    14101 ttttctgctc tgataatcgt aaccgacgcg atacttcacc aatgctcaga ttacgtttga
    14161 tccgttcatc tctcaatatt tgcccaatac tttctggtag tgcgcgatta tttagattat
    14221 ccatctcctc acttcgagac atcattgatt caccgttgct agcaatcatc tcgttgtcac
    14281 cattgttttc attcatctct caatttaccc tcgcgcaatg acctcgcttc attggaatca
    14341 ggaaaacgcc tttgcaactg ggcgccatag ctcgcaactg cttgatcatt tcccaatgca
    14401 tgctcaattc ggatcgctaa ccacaaactg tccgctgaga gcggataaga ctgaatatag
    14461 cgcgtaatat tggtttgagc tactcttaaa tttctactca gatagtcaat ctgaattaat
    14521 cccaataccg ccaagggata atcggggtgg atgacaagcg ctttctgtaa ataagcttta
    14581 gcttgctcaa aatcattgcg cttcaaaaca cacaaacctg cattggtata ggatttctca
    14641 ggtatttcat agaatggatc tttaatcgct tgcataaaat gatcaatagc caaatcaatt
    14701 tgagcaggat tgcgacacag aaaccagcca tagttgttat gtacatccgc atcgttaggt
    14761 gcgattctga ttgcacgttc aaagtttact tgcgcacgcc catcttcacg caaatccata
    14821 tagatcagcc ctaaaacatt ataggctggt gcataattat tattagcacg caaggcaatc
    14881 tctgcttctt caatggcaac acgatactga ccacgactgt aatattgccc cgccaattca
    14941 gtatgaatct tagcgcggtc ttggactcgc ttcctttttt cttcaggtgt aagattactt
    15001 tcaggatcag gtgggctaac acatcctgta agccacgcca aactcaagct aatcaataaa
    15061 gctgttaagc gtttaaacac ttgccattcc ttcagcgcca cgctgagttc gtcgcgtctt
    15121 atccaaaacg ttaccagcta attgaccaca agcagcggcg atatcatctc cccgtgtttt
    15181 acgaacggtg gttaccaacc cagcttgcat caaaacatca cgaaaagaat gaatcgcctc
    15241 cttcgtcgag cgtttataac cggagtcgga aaaggaatta aatggaatca aattaaactt
    15301 acagggagta tctttaacca actctaccaa ttgacgcgca tgctggacgg tatcattgac
    15361 ttgatcaagc atcacgtact caaaagtaat aaaatcccta ggagatgcct gcaaataacg
    15421 cttacaggcc gcaagcaatt catgaagcgg atatttttta ttaattggta ctaattcgtt
    15481 gcgtaattcg tcattaggtg catgcaaaga aacggctaag gctacagggc atcgttctcg
    15541 taaccgatcc atggcgggta caatacccga tgtactgaca gtaactcttc ttctcgataa
    15601 cccataagca ctatcatcga gcattaaatc taacgcagta actacgttat caaaattagc
    15661 caatggctcg cccataccca ttaatacgac attcgtgatt acccgatcag aagctggctt
    15721 ttgttctgta aaacccacta cttcctcatc aggcctatga tcagtctgca ttgaagccgg
    15781 ttgcctctgg gatttaagcg ttttattagc ccaccataac tgcccaatga tttctgcaac
    15841 cgttagattg cgattgaacc cctgcttacc ggttgcacaa aagctacaat taagtgcaca
    15901 acccacctga ctg
//
LOCUS       DAOQLJ010000038        15880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1333_length_15880_cov_6.165434, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000038 DAOQLJ010000000
VERSION     DAOQLJ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 15880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15880
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1333_length_15880_cov_6.165434"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..548
                     /locus_tag="PK522_04325"
                     /pseudo
     CDS             1..548
                     /locus_tag="PK522_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833093.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
     gene            complement(559..1740)
                     /locus_tag="PK522_04330"
     CDS             complement(559..1740)
                     /locus_tag="PK522_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HNC40991.1"
                     /translation="MKRYSFFSLLLLLALSACKTESLSTTKQADTIQPAPSVTENINQ
                     LRLTQWTQLIGWEADELLPAWNTFMQSCTTLKRQPNWQAVCTKASMLEQANEQTIRLF
                     FESQLAPYQVFNSDGSDTGIITGYYEPLLQGSKKSTEKYRFPIYSPPDDLLVIDLGNA
                     LPDFKNLQLRGRLEGRKVVPFYSRAEIEKGIPTLKNRELLWVDDRVALFFLHIQGSGR
                     IQLENGEIIKVGYADQNGHPYVSIGSLLVQRGELPLEKASMQGIKQWGVQNPEKLTEL
                     LHQNSRYVFFRLLPDSLTGPLGSLGVPLTAGRSLAIDPRVVPQGAPVFLSTTWPNSDR
                     SLNRLMVAQDTGSAIKGGVRADFFWGFGKEAENQAGKMKQNGKMWVLLPKDMTIPPHS
                     K"
     gene            complement(1982..2728)
                     /locus_tag="PK522_04335"
     CDS             complement(1982..2728)
                     /locus_tag="PK522_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNC40992.1"
                     /translation="MLQYDLENYLNQLLEISRFRDYCPNGLQVEGHKQINTLISGVTA
                     SLELIEMAIACNADAILVHHGYFWKGEESCIRGMKHQRLSLLLKHKINLFAYHLPLDA
                     HLELGNNIQLARKLGLQYDGTFGEQAIAMHGQLANRMMLGDFGKTIATSLNREPLIIG
                     DLSQKVQRIAWCTGAAQGYFEEAIDARVDAFITGEISEQNVHMARESGVAFIAAGHHA
                     TERYGVQALGTHIAQRFGVNHEFIDLMNPV"
     gene            2796..3932
                     /locus_tag="PK522_04340"
     CDS             2796..3932
                     /locus_tag="PK522_04340"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNC40993.1"
                     /translation="MNKLWLIFAQTVTILLAIFFVVSTLRPELLPWTPRGKIATIKEY
                     TSANPSIIPDSFNAAAETAMPAVVNIFTSKEVRSQTHPQLDDQAFQFFFGDRFSPKTK
                     QTSSLGSGVIVSAEGYILTNHHVVEAASEIQIALVDGRNAKAKIIGSDPESDLAVLKI
                     DLENLPSITIGNSESAKIGDIVLAIGNPFAVGQTVTMGIIGAKGRSQVGINTFENFIQ
                     TDAAINPGNSGGALTDTAGNLIGINTAIWSRTGGSLGIGFAIPVSAAIQIMKQIIETG
                     SVTRGWLGVSMHDVTPELVESLKLKKLGGILITGVLKDGPADQAGIKPGDILQAVDGD
                     EISNSAEVLNLVAALPPGRTVLLTIIRSQSEKSFKVTVGKRPSS"
     gene            3962..5596
                     /locus_tag="PK522_04345"
     CDS             3962..5596
                     /locus_tag="PK522_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC40994.1"
                     /translation="MIKFLNRIYCWLDEVVLSLSREIKLSYLPPLMVYLAAGISSLTA
                     IVGTFYVKERLGLSAEFLAALGFWMALPWALKMPLGHLVDLIWRWKGFLVYLGASLIM
                     LSLLIMIGLLGYPSDMQAIMPIENWYVLAALLAPIGYVLQDVVADAMTVEAVPKVDAN
                     GQAVSQEARKQMHVTMQTLGRVAIIGGGVLVSILNVILMHDMGSLSESEKLATYLFIY
                     QLALVIPIVSVGGVFLASCLRRKNITRLMQQGMKREESIALLDTHAETPPINWWILGG
                     GAAFTVISLSVGLGGISGAEEIVFALSMAIVLFLIKQISGELDEEARNMLVGTAILIF
                     VFRAIPGPGAGSTWWMIDHLGFDQQFLATLSLIGSALTLAGMFIFRRFMAERSMAFII
                     GILTIAGTILSLPIIGMYFGLHEWTAALTGGVVDARFIAVVDTALESPLGQIAMIPML
                     AWIANSAPEKLKATFFAVMASFTNLALSASQLCTKYINQIFIISREVIDASTGEVTIP
                     ADYSQLGLLLMSITAIGFVIPLLTIFILKQTRFRIA"
     gene            5719..6033
                     /locus_tag="PK522_04350"
     CDS             5719..6033
                     /locus_tag="PK522_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HNC40995.1"
                     /translation="MQRFLQLLVIFFVFSSTVFAGVNINTASEAEFEALKGIGPAKAK
                     AIVEYRNQHGHFKSIDDLEKVSGVGPGTINQIRDELIVDEIAAAPDPEKMVVKKEELS
                     SK"
     gene            6178..7062
                     /gene="cysM"
                     /locus_tag="PK522_04355"
     CDS             6178..7062
                     /gene="cysM"
                     /locus_tag="PK522_04355"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HNC40996.1"
                     /translation="MFKTIQDCVGNTPLVQLKRIPGKTSNILLAKLEGNNPAGSVKDR
                     PALSMIMRAQERGAIKPGDTLIEATSGNTGIALAMAAAMMNYQLILVMPENLSIERRQ
                     SMSAYGAKIILTPKEGGMEHARDIAEQLRDEGQGFILDQFANPDNPLAHYKGTAPEIW
                     HETSQSITHFISSMGTTGTIMGCARYFSEANPDIKIIGVQPEEGSQIPGIRKWSENYL
                     PKIYDPTLINRILFVSQTEAETMTKRLAREEGIFTGISSGGALAAALKISDEVEDATI
                     VFIACDRGDRYLSTNVFK"
     gene            complement(7768..10008)
                     /gene="clpA"
                     /locus_tag="PK522_04360"
     CDS             complement(7768..10008)
                     /gene="clpA"
                     /locus_tag="PK522_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HNC40997.1"
                     /translation="MIAQELEASLHMAFIQSRQKRHEFITVEHLLLALLDNPSAAEVL
                     VACAIDIEELRTLLHDHINQHTPIVGGSEDVDTQPTLGFQRVIQRAILHVQSSGKKEV
                     TGANVLAAIFGEKDSHAVYFLQQKGITRLDVTNYISHKICKVSQNNEQNNKAEEESEN
                     EQQSAAPSPLESYTINLNNLAMANRIDPLIGREKELERVIQTLCRRRKNNPLLVGEAG
                     VGKTAIAEGLAKRIVEGDVPEILSKHQVYALDIGALLAGTKYRGDFEQRFKTILKQLT
                     DNPKAILFIDEIHTLIGAGAASGGTLDASNLLKPILSSGRLKCIGATTYNEFRGIFEK
                     DHALSRRFQKIEVNEPSIDETVAILRGLKSHYEKHHGVKYSSIALTSAAELSARFIGD
                     RHLPDKAIDIIDEAGAAQRILPKSKQRKVIGKTEIEEIIAKIARIPSQSISSDDRSKL
                     KTLDRDLKSIVFGQDAAIDVLAIAMKLSRCGLGNPQKPIGSFLFSGPTGVGKTEVARQ
                     LAYIMGIPLHRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTETIIKHPHSVLLLDE
                     IEKAHTDIFNVLLQVMDHGTLTDNNGRKADFRNVIIIMTTNAGAESLVKSSIGFTETS
                     QTGNEMTEITRLFTPEFRNRLDSIISFAPLSKGIILRVADKFLMQLETQLQEKKVEVI
                     FTDDLRNYLAEKGFDPLMGARPMARLIQDVIRSALADELLFGKLTNGGKVTVTISKEG
                     KVELQF"
     gene            complement(10005..10319)
                     /gene="clpS"
                     /locus_tag="PK522_04365"
     CDS             complement(10005..10319)
                     /gene="clpS"
                     /locus_tag="PK522_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HNC40998.1"
                     /translation="MTIRNNREESVLEDNKNKLKTPPLYKVLLLNDDFTPMDFVVMVL
                     QTFFFMSQEKAIHVMLKVHNEGAGICGVYPSDIAMTKVKQVNDFSRQNQHPLKCVMEK
                     NT"
     gene            10667..10870
                     /locus_tag="PK522_04370"
     CDS             10667..10870
                     /locus_tag="PK522_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNC40999.1"
                     /translation="MATGTVKWFNDSKGFGFITPDDGSEDLFAHFSAINMTGFKTLKE
                     GQKVSFEVTQGPKGKQASNIQAA"
     gene            complement(10991..12241)
                     /gene="icd"
                     /locus_tag="PK522_04375"
     CDS             complement(10991..12241)
                     /gene="icd"
                     /locus_tag="PK522_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNC41000.1"
                     /translation="MFQHIKIPAEGKKILVNSDQTLNVPDNPIIPYIEGDGTGVDITP
                     VMLKVVDAAVSSAYGGKRKIAWMEIYAGEKSTKVYGENVWLPDETLQAAKEYVVSIKG
                     PLTTPVSGGIRSINVALRQLLDLYVCLRPVRYFQGVPSPVKQPEKVDMVIFRENSEDI
                     YAGIEWEAESSAANKVIRFLIDEMQVKSIRFPDTSGIGIKPVSREGTERLVHKAIQYA
                     IDNKRSSVTLVHKGNIMKFTEGAFKNWGYHVAAKDFGAELYNGGPWMRLPQDKGGIII
                     KDVIADAFLQQILLRPEEYDVIATLNLNGDYISDALAAQVGGIGIAPGANLSDSIAIF
                     EATHGTAPKYAGKDQVNPGSIILSAEMMLRHMGWNEAADAILRAMEKTITSKIVTYDF
                     ARLMDGATEVSCSKFGSTLIANMS"
     gene            complement(12320..12808)
                     /locus_tag="PK522_04380"
     CDS             complement(12320..12808)
                     /locus_tag="PK522_04380"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HNC41001.1"
                     /translation="MDNRFLWGFMAALVIWVSIFAVVYFFFASAQAPIVAMVNTDSVE
                     KEIVIPRSKDGHYYVRGKINGYPVDFMVDTGASVVSISYDLARSIHLPRGVPANFSTA
                     GGNMIGEIVSAIDIEVGNIRLSELTVSVGIQGNIALLGQNFLRRIDVIQSDDKMILRI
                     RN"
     gene            complement(12939..13682)
                     /locus_tag="PK522_04385"
     CDS             complement(12939..13682)
                     /locus_tag="PK522_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41002.1"
                     /translation="MENPLTKSYLSTHSVNAWKNTLTNLVRRSQLVVVSKLKRRRRYT
                     LVLHRLKRNLVLAGGLIGFMVGLSWITINYLAKESSYLSTEDQESYSIVVPPHVFIDS
                     IDTLEKWLRKELKLPYAKSHMIIRPASVLSGNKIAFTQLPESWEIKFLTHNSNFSVRY
                     WKGDTDVLVIKREASFWALLKNIHDDSTSNSAWVLLTDAVVGGVSSYVVINSLIWSRL
                     YYLRLLAVGFIGITLAISFGLVLNSFSLY"
     gene            complement(14137..>15880)
                     /locus_tag="PK522_04390"
     CDS             complement(14137..>15880)
                     /locus_tag="PK522_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC41003.1"
                     /translation="RLKPRYSLSNLLADLQSQGLLQLSHSQAVGTDYQSKGAMRRETP
                     EAIISAMYDLSGLPAQEGALLSVFAVLPAESIPFARLETLLPGLPELDTCLLSLAQKG
                     WIEYNEATISFKCSPVVQEVTRQKNPGLREDCTMLVNTLIEKLGYDGRHMTGSSYDEA
                     HLYARYAEAVMKTFLMKPDDNLLALSERTGNYHRITGNLERSLFFFEHLTQVCKTLLE
                     GAPDDPALKNNLATSYSELGTTHKAMGNLQQALTCFEQSNRLGKELFDTYPQDALCKG
                     FLAISCQYLGDTHKAMGNLEQALTFFEQSNQLEKALSDAAPQDVGFKHELAISCQHLG
                     ITHKALGNLKEALDFFEQYHRLEKELVDVYPQNVEFKYNLAASYQFLGSTHTALGNLK
                     EALDFSGQYHRLEKELADAYPQNVEFKDSLAISCQHLGITHTALGNLKEALDFFEQYH
                     QLEKELADVYPRNVGFKNGLALSCQCLGGTHQALGNLKEALDFFEQYHQLEKELADVY
                     PRNVEFKNNLALSYQWLGWFYEDKLQDLNQGQAYYRLSKSLLLELVSSFPGYVQFKQS
                     LDWVTSRLPDEADE"
BASE COUNT         4616 a         3444 c         3122 g         4698 t
ORIGIN      
        1 gccagtttat ggaaaatttt tgctgggaat gggacgtatt gagtcaaatg accgctcata
       61 tcgaaagtgg agtaccgctc ccacgtgctt tatttgacta attactggct gccaaaaatt
      121 tccagagtgg tctacaaaca ttacgccaga ttgaatttgc gctgttcgat atgcatctac
      181 actatgattt cgatcctaat ggcgaaaaga ccgtattaac attactcgat gaagtgcgtc
      241 agcaggttgc agttgttatt ccacccgcgt ttagtcgttt tcccaacagt tttgcgcata
      301 tttttgcagg tggatacgct gccggatatt atagctacaa atgggctgag gtcttatcag
      361 cagatgcgta cagtttattt gaagaaaccg atccaagtaa tatcgtcaac actcaagctg
      421 gtagtcgctt ctggagcgaa attctagcgg tgggaggaag tcggcctgca ctagagtcat
      481 ttatcgcatt tcgaggacgg gaacctaaaa ttgacgcttt gctccgccat aatggcatga
      541 ccacttgaga atcttgagtt atttcgaatg aggtggaatg gtcatatctt tcggtagtaa
      601 tacccacatt ttaccattct gcttcatttt tcctgcttga ttctcggctt ctttaccaaa
      661 tccccagaaa aaatcggcgc gtacgccccc tttgatggcg ctaccggtat cttgagcaac
      721 cattagtcgg tttaatgatc tatcactatt aggccaggtc gtcgaaagaa aaacaggcgc
      781 gccctgaggt acaactcggg gatctatggc aagactccga cccgccgtca atggaacacc
      841 cagtgaacct agtgggcccg ttaatgaatc aggaagtaat cgaaaaaaaa cataacggga
      901 attctgatgt aataactcag ttaatttttc tggattctgt acaccccatt gtttaatacc
      961 ttgcatcgat gctttttcta atggtaattc accgcgctga actaaaagtg acccaatcga
     1021 aacataggga tggccattct gatccgcata acctacctta ataatttctc cattctcaag
     1081 ctgtatacgc cctgaaccct gaatatggag aaaaaaaagc gctacccgat cgtctaccca
     1141 aagtaattct cgatttttta gcgttggaat ccccttttca atttctgcac gactatagaa
     1201 aggaacaacc ttacgacctt caaggcgtcc tcgaagctgt aaattcttaa aatcgggaag
     1261 tgcattaccg agatcaatga cgagtagatc atcgggaggt gaataaattg ggaaacgata
     1321 tttctcagtt gattttttac taccttgcag caagggttca taatagcctg tgataatccc
     1381 agtatcactg ccgtctgaat taaatacctg atacggtgca agttgacttt caaagaataa
     1441 tcgtatggtt tgctcatttg cttgctcaag catactggct tttgtacaaa ctgcttgcca
     1501 gttaggttgg cgctttaatg tagtgcagct ttgcatgaat gtattccaag caggcaatag
     1561 ctcatccgct tcccaaccga ttaactgtgt ccattgtgtt aacctaagtt gattaatatt
     1621 ctcagtaaca cttggagctg gttgtatcgt atctgcttgc ttggttgttg aaagcgattc
     1681 ggttttacat gcgctaagcg cgagtaatag taataaacta aaaaacgagt atcttttcat
     1741 caaattcacg ttagtggtat gctgttatta cgattttatt aaaacaatca agaaaataat
     1801 atgtgggtat tagtggttga agcagcactt gcgttggggc tttttctttt cattatctgg
     1861 tggacgattc ctaaaaaaaa gaaggataaa gataagcctt aaaaatagtc cgatacgatg
     1921 agcactacca gtttatctaa tcagaacccc tatgctgcat agcgcccgct agcgattctg
     1981 gtcatactgg attcattaaa tctataaact catggttgac tccaaaccgt tgcgcgatgt
     2041 gcgtaccaag cgcctgaacg ccataacgtt ccgttgcatg gtgccctgca gcaataaacg
     2101 cgacccctga ttctctagcc atatggacgt tttgttcgga aatttctcca gtaatgaaag
     2161 catcaacccg agcgtcaatc gcttcttcaa aataaccttg tgctgcaccg gtacaccatg
     2221 caatacgctg caccttttga gataaatcac caataatcaa cggttctcga ttaagagaag
     2281 tcgcaatagt cttcccaaaa tcaccaagca tcatacgatt agccaattgc ccatgcatcg
     2341 caattgcttg ttccccaaaa gtaccatcgt actgcaaacc tagttttcgt gctaactgaa
     2401 tattattccc caactccaaa tgtgcatcga gcggcaaatg atacgcgaaa agattaattt
     2461 tatgtttcag taacaaactg aggcgttgat gtttcatacc tcgtatgcaa gattcttccc
     2521 ctttccagaa ataaccatga tgcacaagaa ttgcatcagc attgcaagca atagccatct
     2581 ctatcaattc cagagaagcc gtaacgccgc taatcaaagt atttatttgc ttatgccctt
     2641 ccacttggag tccatttggg caataatcgc gaaagcgcga aatttccagt aattgattaa
     2701 gataattttc tagatcatat tgcaacattg tttacttcca acctgtcagt tatatattta
     2761 atattcgctt tattgtctta ggtatgaatt tgcaaatgaa taaactctgg ttgatttttg
     2821 cacaaactgt cacgattttg ttggctatct tttttgttgt ttccacattg cgaccagaac
     2881 tacttccatg gacgcctcgt gggaaaatcg ccacaattaa agaatatact tccgcgaatc
     2941 cctctatcat acctgatagt ttcaatgctg ccgctgaaac tgcaatgcct gcggttgtta
     3001 atatctttac tagtaaagaa gttcgatcgc agactcatcc tcaactggat gatcaagcat
     3061 ttcagttttt ctttggtgat cgctttagcc ccaaaacgaa acaaacatcc agccttggtt
     3121 ctggtgttat tgtcagcgct gaaggctata ttttgacaaa tcatcatgtt gtagaagctg
     3181 caagcgaaat acaaatcgcg ctggtagatg gtcgaaatgc taaggcaaaa attattggct
     3241 ctgatccgga aagtgattta gccgtcttaa aaattgatct agaaaaccta ccaagtatta
     3301 ccatcggcaa ctcggaatct gcaaaaatag gcgatattgt attagcaatt ggcaatccct
     3361 ttgcggttgg tcaaacggta acaatgggaa ttataggtgc aaaaggtcgg tcacaagttg
     3421 gcatcaatac gtttgaaaat ttcatacaaa cggatgctgc gattaaccct ggtaattcag
     3481 gcggtgcttt aacggatact gcaggcaacc taattggaat caatacagca atctggtctc
     3541 gaacaggggg atcactgggt attggattcg ccatccctgt tagcgcagcc atacaaatca
     3601 tgaagcaaat catcgaaact gggagcgtga cgcgagggtg gcttggtgta agtatgcacg
     3661 atgttacacc tgagttggtt gagtccctca aattaaaaaa gctagggggt atcctcatta
     3721 caggtgtgct caaggatggt ccagccgatc aagcgggtat aaaacctggc gacatcttac
     3781 aagcagtcga tggtgacgag attagtaact cagcagaagt actgaactta gtcgccgctt
     3841 taccccctgg tagaaccgtt ttgttaacca ttattcgaag tcaatcggaa aaatcattca
     3901 aagtgacagt cggtaagcgt ccatctagtt aataatctaa aacgatagta ataaaattca
     3961 attgattaaa tttctcaatc gcatttattg ttggcttgat gaagtagttc tttcactgag
     4021 ccgtgaaatt aaattatctt atctaccgcc gctcatggtt tatttagcag ctggtatttc
     4081 aagcttaact gccattgtag gtacttttta tgtcaaagaa cgattaggat tatcagcaga
     4141 atttttagct gcattaggat tctggatggc cctaccttgg gcattgaaaa tgccacttgg
     4201 tcacttagtc gatttaattt ggcgatggaa gggctttctt gtctatctgg gtgccagctt
     4261 gatcatgctc agtttgttga tcatgatcgg attactggga tatcctagcg atatgcaagc
     4321 cataatgcct atcgaaaatt ggtatgtgct agctgcatta ctcgctccaa tcggctatgt
     4381 gcttcaagat gtggtcgcag atgcgatgac cgttgaagct gttcccaaag tagatgctaa
     4441 tgggcaagcg gtctcacaag aagctcgtaa acaaatgcat gtcaccatgc aaacactggg
     4501 gcgagtagca attatcggag ggggggtact ggtctcgatt ctgaatgtca ttctgatgca
     4561 cgatatgggt tctttatctg aatcagaaaa attagcaact tacttattta tttaccaact
     4621 tgcgctagtt atccccattg tatctgtcgg tggagtattt ctagcaagct gcctcagaag
     4681 aaaaaatatt acccgtttaa tgcaacaagg aatgaagcgt gaagaatcga ttgccctatt
     4741 agatacgcat gctgagaccc ctccaatcaa ttggtggatt ctggggggag gtgccgcctt
     4801 cacagtcatc tcattaagtg taggattagg tggtattagc ggcgcagaag agattgtttt
     4861 tgcattatca atggccatcg ttctttttct aataaagcaa atctcgggcg aattagatga
     4921 agaagcgcgc aatatgctgg ttggaacggc aattctaatc tttgtatttc gagccatccc
     4981 tggaccgggt gctggctcaa cttggtggat gattgaccac cttggctttg accaacaatt
     5041 tctggcaacg ctctcattaa tcggttccgc cttaacgctc gcaggtatgt ttatctttcg
     5101 acgctttatg gcagagcgat cgatggcttt tattatcgga atactgacaa ttgcaggcac
     5161 catcctttct ttgcctatca ttggaatgta ctttggattg catgaatgga ctgccgcgtt
     5221 gacagggggt gttgttgatg cgcgctttat tgctgtcgta gacacagcgt tagaatcacc
     5281 tttagggcaa atcgctatga ttcccatgct agcctggatt gccaattcag cccctgagaa
     5341 actcaaagct acctttttcg ccgttatggc atcttttact aatcttgcac tatcggcatc
     5401 tcaattatgc acaaaatata tcaatcaaat attcatcata tccagagaag ttattgatgc
     5461 cagtacaggt gaagtcacta ttcctgctga ttatagtcaa ttaggtttat tactgatgtc
     5521 tattaccgct attggttttg tgattccttt gcttacaatt tttatcttaa aacaaacacg
     5581 tttccgaatt gcctaatcct tttatttgat ttttcatcct agcctaaaac tccacaacga
     5641 tctgccgata tattctggcc ttttgattgc acgatttgta tttcacttgg caatcttttt
     5701 taaacgatag gagattttat gcaaagattt ttacaactat tagtaatctt ttttgtattc
     5761 agtagtactg tttttgctgg cgttaatatc aacacagcat ctgaagcgga gtttgaagct
     5821 ttaaagggaa ttggaccagc taaagcaaag gctattgtag agtatcgaaa tcaacatggc
     5881 catttcaaat ctattgatga tttagagaaa gtaagtggcg ttggtccagg caccattaat
     5941 caaatccgcg acgaattaat cgttgatgaa attgccgctg cacctgatcc cgaaaagatg
     6001 gttgtaaaaa aagaagagct gtcttctaag taaataaagc tagcttcatc aaaaccctct
     6061 gaaatataat ttcagagggt ttttgctttt ttattaaccc tatgctttat caagtctgta
     6121 ccctgtatgt aataattcca tttattcatt ggtatttaaa aaaatccaaa ctcacctatg
     6181 ttcaaaacaa ttcaagattg tgttggcaat accccactcg ttcaacttaa acgtatacca
     6241 ggaaaaacca gcaatattct tttagcgaag ttagaaggta acaacccagc aggatcagtt
     6301 aaagatcggc cagcactttc aatgatcatg cgtgcacaag agcgtggcgc tattaaaccg
     6361 ggtgatactt tgattgaagc aaccagtggg aataccggca ttgcattagc gatggctgct
     6421 gctatgatga attatcagtt gattttagta atgccagaaa atctgagtat tgaacgacgc
     6481 caatcaatga gtgcatacgg cgcgaaaatc atcttaactc ctaaagaagg gggtatggaa
     6541 cacgccagag atattgccga acaactacga gatgaaggtc aaggatttat tcttgaccaa
     6601 tttgctaatc ctgacaaccc attggcccac tacaaaggca cagcacctga aatttggcat
     6661 gaaaccagtc aatcgattac ccattttatc agcagtatgg gcacaacagg aacgattatg
     6721 gggtgcgctc gatatttttc cgaagctaat ccagacatta aaattattgg tgtacaacct
     6781 gaagaaggat ctcaaatacc tggtatcaga aaatggtctg aaaattacct tcctaaaatt
     6841 tacgacccaa cactcattaa tcgtatcctc tttgtctccc aaacagaagc tgaaacgatg
     6901 acaaaacgcc tagcacgcga agaaggaatt tttaccggta tttcatcagg cggtgcactc
     6961 gctgctgcac ttaaaatttc agatgaagtc gaagatgcta ctatcgtttt catagcttgt
     7021 gatcgaggtg atcgttatct atctactaac gtgtttaaat aattgtcctt gtaaatacat
     7081 ccacacccca gatataaagt gtaaatggtt gtatgcttgc agatcaaatt gactaatatt
     7141 gtcttaccac agtacgaaag tagtctattt gcttaaacac attggtcgag caaattttct
     7201 gaataagtat tgatttacat gggcctaaga cgttggaaga tacggttctt attgatacga
     7261 cgctacaaaa aaagaacagt acctatctta ctgacagcaa actggcgatc aaaatcatta
     7321 atcgcctgaa caagatctac tcgctgcgtg tacaggagaa aagaccacaa gcaatacgaa
     7381 tatggcagca aggcatcgat tgccagtacc gtacaagatc atttaatcgt cagcgtgatc
     7441 aatcacgaat aaaacctgca tgacagtcat acgttaccgg aaatatttta tcatgttgca
     7501 gcatcgcgcg gcaaaaggga agtacttctt gaaaagagtt attggtcgat catatcaatt
     7561 tattgatagt cgcctacgcc tggaatttaa gagctatggc tactggccac tttttgactg
     7621 tttttccaag gaaacagcag taaatttcat cttcttccaa tgagaataat aaaaaccatt
     7681 ttccccaaga aggggaagat caggcccaat ttttcaatct ttataacact tgtttgaata
     7741 ttcataccaa cattaatctt gtgtgcctta gaattgaagt tctactttac cttcttttga
     7801 aatggtaact gtcactttcc ccccattcgt caatttcccg aagagtaatt catcagccaa
     7861 ggcactgcga ataacatctt gaatcaaacg agccatagga cgggcgccca ttaaaggatc
     7921 aaatcccttt tctgcgagat aattgcgcaa gtcatcagta aaaataactt caactttttt
     7981 ctcttgcaat tgagtttcaa gctgcattaa aaacttatct gctacacgta aaataatacc
     8041 tttacttaat ggtgcaaatg aaataattga atctaatcga tttctaaatt caggtgtaaa
     8101 taagcgcgta atctcagtca tttcattacc ggtctgcgaa gtttccgtaa aaccaatact
     8161 tgatttcacg agtgattcag ccccagcatt agtagtcata ataatgatga cattacgaaa
     8221 atccgctttc cgcccattat tatcggttaa tgtgccatga tccatcactt gaagtaatac
     8281 attaaaaata tcagtatggg ctttttctat ttcatcaagc agcaataccg agtggggatg
     8341 cttgataatc gtttcggtca ataaaccacc ttgctcaaat ccgacataac ccggtggcgc
     8401 tccgattaga cgtgaaacag catgccgctc catatactcg gacatatcaa aacggtgcaa
     8461 tgggattccc atgatatagg cgagttgacg tgccacttcg gtcttaccga cacccgtagg
     8521 accggagaaa agaaaagatc caattggttt ttgcggattt cctagaccgc atctcgataa
     8581 tttcatagca attgcaagca catcgattgc ggcatcttga ccaaatacga ttgatttcaa
     8641 atcacgatcg agcgttttga gtttactacg atcatcgctt gaaatgctct gagagggaat
     8701 tcttgcaatc ttagcgatga tttcttcaat ttcggttttg ccaatcactt tacgttgctt
     8761 agattttggt aggatacgtt gtgctgcacc tgcctcatca ataatatcaa ttgctttatc
     8821 aggcaagtgc cgatcaccaa tgaatcgtgc tgaaagctct gctgctgatg ttagtgcaat
     8881 cgacgaatat ttcaccccat gatgcttctc ataatgagac tttaaacctc gtaatatcgc
     8941 taccgtttca tcaatgctcg gctcgttgac ctcaattttc tgaaagcggc gtgatagcgc
     9001 atgatctttc tcaaaaatac ctctaaattc gttataagtt gttgcaccaa tgcatttcaa
     9061 tctcccagaa ctcaatatcg gtttaagtaa attcgatgca tctaacgtac ctcccgaagc
     9121 tgcgccagca ccgatcagtg tatgaatttc atcaatgaat agaatggctt ttggattatc
     9181 agttaattgc tttaggatag ttttaaaccg ttgttcaaaa tcaccgcgat atttagtgcc
     9241 agcaagaagc gctccgatat ccaatgcata aacttgatgt ttagacaaaa tctcaggtac
     9301 atccccttct acgattctct tggctagacc ctcagcaatc gctgttttac caacacccgc
     9361 ttccccaacc agtaacgggt tatttttccg acgccggcat agggtttgaa taactcgttc
     9421 taactctttt tccctaccaa ttaacggatc aatacggtta gccattgcca ggttattcag
     9481 attaatcgta taactttcta atgggctagg cgcagcagac tgctgctcgt tctcactttc
     9541 ttcttcggct ttattatttt gctcgttatt ctgcgaaacc ttgcagattt tatgggatat
     9601 gtaattcgtt acatccagtc tcgtaatacc cttttgttga agaaaataaa ctgcatgcga
     9661 atctttttcg ccaaagattg cggctaaaac atttgctcct gtaacttctt tctttccaga
     9721 tgattgaaca tgcaagattg cgcgctgaat tacccgttgg aatccaagtg ttggttgtgt
     9781 atctacgtct tcgcttcccc ccacaatagg ggtatgttga ttaatatgat catgtaaaag
     9841 tgtgcgcaat tcctcaatat cgattgcaca agcaactaat acttcagccg cacttggatt
     9901 atcgagtagc gcaagcagta aatgttctac tgtaataaat tcatgtcgct tctggcgtga
     9961 ttggataaac gccatatgga ggctagcttc taattcttga gcgatcatgt atttttctcc
    10021 ataacacact ttaatggatg ctgattttgc ctagaaaaat catttacttg tttcacttta
    10081 gtcattgcaa tatcactcgg ataaacacca caaattcctg cgccctcatt atgaacttta
    10141 agcataacgt gaatcgcttt ttcttggctc ataaaaaaga aagtttgaag taccattacg
    10201 acaaaatcca ttggtgtaaa atcatcatta agtaacagca ctttatacag cggaggtgtt
    10261 ttgagcttat ttttattatc ctctagaaca ctttcttcgc gattatttct tattgtcatc
    10321 actgattcct ttttgccaag ctaatatcta aaaactttag actacaggga tttaatcata
    10381 agtcttcatc aacattagca ttctaattat ttttcttttt acatatttga cgattacgac
    10441 aaaattttca agcgtattag ctgcattcac ttattttact gctacttaaa aaaagtataa
    10501 aagtgatatt ttcaaatgct aaacgcttga cttaagatta aaatttgcgt aaaactgcaa
    10561 ctggcgtttg gcttcaagtt ccctgcagta ttggttagag ccgtttgcgg tcttgaaatt
    10621 caaatagttt tagggtttcc ggcccagttt tttataggaa gtagagatgg caacaggtac
    10681 agtaaaatgg ttcaatgatt ctaaagggtt tggttttatt actcctgatg atggaagcga
    10741 ggatttattc gcccatttct cagcgattaa tatgaccggt tttaaaactc tgaaagaagg
    10801 ccaaaaggtg agctttgaag taactcaggg tcctaaagga aaacaggcat caaatattca
    10861 ggctgcttaa ggtagattgt ttggtctact ttgattagtt tattttagct ataaagcaac
    10921 ttgcccatta ctataatact gttaatcatt attatgtaat gggcaaaaca taaattctaa
    10981 gaacaataca ctaagacata ttagcgataa gtgtacttcc aaattttgaa caagaaacct
    11041 cagtagcgcc atccattaac cgagcaaaat cataggtgac aattttgctt gttatggttt
    11101 tctccatcgc cctcaagatt gcgtcggctg cttcattcca gcccatatgc cttagcatca
    11161 tttcggcgga aaggatgatt gatccaggat tgacttgatc ttttccggca tatttaggtg
    11221 ctgttccatg agttgcttca aaaatagcaa tcgaatcact aagattcgca cctggtgcaa
    11281 tcccaatacc accaacctgt gctgctaacg catctgaaat ataatcacca tttagattta
    11341 aagtggcaat gacgtcatat tcctctggcc gcaataagat ctgttgtaag aaagcatctg
    11401 caatcacatc tttgataatg ataccccctt tatcctgagg tagtctcatc caaggcccgc
    11461 cattatagag ctctgcaccg aaatctttag ccgcaacatg ataaccccaa tttttaaatg
    11521 ccccttcggt aaatttcata atattgccct tatgcaccag tgttaccgaa gatcttttgt
    11581 tatcaattgc atattgaatc gctttatgaa ctaacctttc tgtaccttct cgcgagactg
    11641 gtttaatgcc aatccccgat gtatcaggaa aacgaatgct tttcacctgc atttcatcga
    11701 taagaaaacg aatcacttta ttcgccgctg aagattcagc ctcccactcg atgcccgcat
    11761 aaatatcctc agagttctcc ctaaaaatca ccatatcaac tttttcaggc tgtttaacag
    11821 gactgggtac tccctggaaa taacgtactg ggcgtaagca tacatacaaa tctagcaact
    11881 gccttaatgc aacattaata gaacgaatcc ctccactgac tggcgtagtt aatggtccct
    11941 taatagaaac cacatactct ttagctgctt gtaatgtctc atcaggtagc caaacattct
    12001 ctccatagac tttggttgat ttctctcccg cataaatttc catccaggcg atttttcttt
    12061 tacctccgta ggcgctcgaa acggcagcat caactacttt taacataaca ggtgtgatgt
    12121 caacaccggt tccatcacct tctatatagg gaataatagg gttatccggt acattcaacg
    12181 tttggtcaga attaacaaga atttttttac cttccgcagg tatttttata tgctgaaaca
    12241 tattagtctc cgatcatctc gctcgtgtta gattagtaac atcatcaatt caatttttgt
    12301 tcaagctaaa gctatttttc taatttctaa ttcgcaatat catcttatca tcagattgga
    12361 tcacgtcgat tcttctgaga aaattctgcc caagcaatgc gatatttcct tgaataccca
    12421 cactaacagt tagttcgctg agtctaatat taccgacttc gatatcgatt gctgaaacaa
    12481 tctctccgat catattacca cccgcagtag aaaaatttgc gggaactccg cgcggtaaat
    12541 ggatggaacg agccaaatca tagctaatcg atacaacgct agcacccgtg tcaaccataa
    12601 aatcaacggg ataaccattg attttacctc taacgtaata gtgaccatct ttggagcgag
    12661 gaataacgat ttctttttct acagaatccg tattgaccat cgccactatc ggcgcctgtg
    12721 ccgatgcgaa gaaaaaatat acgacagcaa agatactcac ccaaatgacg agcgctgcca
    12781 taaatcccca caaaaatcga ttatccataa aaattatcaa gtcacaattt taaaataaga
    12841 aaataatcga atcattgcag caagaaatag cttggatagg ttattgtaca ccttctaccc
    12901 taacagtcac gaaagaaccc agagtcaata acgctgatct aatatagcga gaaggaattt
    12961 aaaacgagac cgaatgaaat cgccaacgta ataccaataa aaccaactgc aagcaatcgg
    13021 agataatata aacgactcca aatcaaagaa tttattacca cataagacga taccccccct
    13081 acaacagcat cagtaagaag cacccaagca ctattgctcg tcgaatcatc atgaatattt
    13141 tttaacaatg cccagaaact agcttctcgc tttataacta acacatcggt atcacccttc
    13201 caatagcgaa cactaaagtt actattatgt gtcaaaaatt tgatttccca cgattcaggc
    13261 aactgcgtga atgcaatttt attgccggac aatacgctag caggtctaat tatcatgtgc
    13321 gatttagcat aaggcagctt taattcctta cgtaaccatt tttctagtgt atcgattgag
    13381 tcaatgaaaa catgtggggg aacaacaatc gagtaggatt cctgatcttc tgttgataaa
    13441 taactacttt ctttagctaa ataattaatt gttatccaag aaagccccac cataaaacca
    13501 attaagcccc ctgctagcac caagttgcgt tttaatcgat gtaataccag cgtataacgc
    13561 cttctgcgct ttaacttgga aacaacaaca agctgactcc gccgtactaa attagttagc
    13621 gtattcttcc aagcatttac gctatgagta gaaagataag atttagttaa tggattttcc
    13681 atcgagttaa caatgcgtga agcacacgtt gcgtaataaa gtagtgacta ctgagtccaa
    13741 gcaattcatg atcgtgagta gagcagatat gccttcatta ctgaaataat gcatacccaa
    13801 ccagatttac agcacagttg aaataaaaga cttaatcaaa gagcagcaca accacaccgg
    13861 attaaaagga tactgcgaag tgaaaaatat ccttgcaaga ataattgtgc tttttcactg
    13921 gcttttattg aaattgttaa atttctactg agcgcaaaag caaatagaac gacatacgaa
    13981 ggtctgtgaa actagatgga gattaagtga ttcttaccgt aagcctccga ttccgtcagg
    14041 ctacccaaat tcttctcctt agagcttcaa tagcttccgg tgcaaacgac agcaaatcat
    14101 cgatgcgact gtttagccaa gtagagagta gtcactttat tcatcagctt catcgggcaa
    14161 tctactggtc acccaatcca ggctttgttt gaactgcaca taacccggaa aactgctgac
    14221 taattccagt aacaagctct ttgataggcg gtaatacgct tgcccctgat tcaaatcctg
    14281 aagcttatcc tcataaaacc aacccagcca ttgataggat agcgccaggt tatttttgaa
    14341 ctccacattt cgcggataga cgtcagccag ttctttttcc aattggtggt attgttcaaa
    14401 gaaatccaat gcctccttga gattgcctag tgcttggtgc gtgccaccaa ggcattggca
    14461 tgaaagtgcc aagccgtttt tgaatcccac atttcgcgga tagacgtcag ccagttcttt
    14521 ttccaattgg tggtactgtt caaagaaatc caacgcctcc ttgagattgc ctagtgccgt
    14581 gtgcgtaatg ccaaggtgtt ggcacgaaat cgccaagctg tctttgaact caacattttg
    14641 cggataggcg tcagccagtt ctttttctaa tcggtggtac tgtccagaaa aatccaatgc
    14701 ttccttgaga ttgcctagtg ccgtgtgcgt gctgccaagg aattgatatg aagccgctaa
    14761 gttgtatttg aactcaacat tttgcggata aacgtcaacc agttcttttt ctaatcggtg
    14821 gtactgttca aagaaatcca acgcctcctt gagattgcct agtgccttgt gcgtaatgcc
    14881 aaggtgttgg cacgaaatcg ccaactcgtg tttgaatccc acatcttgcg gagcggcgtc
    14941 agacagtgct ttttctaatt ggttggactg ttcaaagaaa gtcagcgcct gctctagatt
    15001 gcccatggcc ttgtgcgtgt cgccaaggta ttggcacgaa atcgccagga agcctttgca
    15061 taacgcatct tgcggatagg tgtcaaataa ttcttttccc aatcggttgg attgttcaaa
    15121 gcaagtcagc gcctgctgga gattgcccat ggccttgtgc gtcgtgccaa gttctgagta
    15181 cgaagtcgcc aagttgtttt ttaacgctgg gtcatcaggg gcaccctcaa gcagtgtctt
    15241 gcagacttga gtaaggtgtt caaaaaagaa cagactgcgc tcaaggttgc cagtgatgcg
    15301 atgatagtta ccggtgcgct cgctcaaagc gagcagatta tcatccggtt tcatcaagaa
    15361 agttttcatc actgcttcgg catagcgagc gtacaagtgc gcttcgtcat aggaagagcc
    15421 cgtcatatgc cggccatcat aacccaattt ttcaatcaac gtgttcacca gcatcgtgca
    15481 gtcctcgcgc aagccgggat ttttctgccg ggtcacctct tgtaccacgg ggctgcattt
    15541 aaaggaaatg gttgcttcat tgtattcaat ccaccctttc tgagcgagag aaagcaggca
    15601 ggtatcgagt tcaggcaagc cgggcagcag tgtttcaagc cttgcgaaag gaatactttc
    15661 cgccggcagc acggcaaaaa ccgagagcag cgcgccttct tgcgcaggca atccgctgag
    15721 atcgtacatg gcagagataa tggcttccgg ggtttcccgg cgcattgcac ccttgctctg
    15781 gtaatcagtg cccacggctt gcgaatggct gagttgcagc aaaccctgac tttgcaggtc
    15841 ggcgagcagg ttggacagac tatatcgtgg cttgaggcgg
//
LOCUS       DAOQLJ010000039        15800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1345_length_15800_cov_5.422801, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000039 DAOQLJ010000000
VERSION     DAOQLJ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 15800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15800
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1345_length_15800_cov_5.422801"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..1635
                     /locus_tag="PK522_04395"
     CDS             256..1635
                     /locus_tag="PK522_04395"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HNC41004.1"
                     /translation="MFISVLDLFKVGIGPSSSHTMGPMRAAHDFLSRIDEISSSFSRQ
                     SVYSVRCILKGSLAYTGKGHATDRAVTLGLHNYLPEELAKEDVNALVTRLELEDTLVL
                     KNGVKLKFKPDEDIVFDKGEPLPQHPNGMIFTVVDENKKIWLTETYFSIGGGFINTLA
                     EIDQLNAPLKMETAASCPYPFDSARSMLRMSRESTLSIAEMKKANELQKITADELNQG
                     LDKIWSAMQQCVENGLKAEGRLPGGLKVERRAKKLYNQLNQDPIKATLNDWLCAYAMA
                     VNEENAAGHLVVTAPTNGAAGVIPAVLYYMTRHENGSIENVRDFLLTAAAIGGIIKHR
                     SSISGAEVGCQGEVGSAAAMAAAGLCAAQGGTAQQIENAAEIALEHHLGMTCDPVGGL
                     VQVPCIERNGFGAIKAHTAAALAKRGTGEHFMPLDSCIAAMKQTGLEMSHKYKETSLG
                     GLAVSVTEC"
     gene            1724..2965
                     /locus_tag="PK522_04400"
     CDS             1724..2965
                     /locus_tag="PK522_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNC41005.1"
                     /translation="MSEAIQQNNESRSKTLPAIEPLTTLEYGGNKITILGTAHVSQAS
                     ADKVKELIATGQYDAVAVELCPSRYKAIVNPDALAKMDLLQVIKKGQASMVAASLALG
                     AFQQRMAEQLGVEPGSEMRTAIKDATDAKLPVLLIDREIGTTLKRIYYNVPWWRRFEL
                     YSGLLASIITRESVSSEEIEKLKEGDVLESAFLQFSQDQKDLFKPLISERDEYMSARL
                     INECKENNYKHTLAIVGAGHLKGIKELLMNRSISNTNQKLEELDTIPNTSSWFKYLPW
                     IIVILILTGFAFGFDRSSDIGMAMIIDWVFINGGLAALGAIIALAHPLTILTAFLAAP
                     ITSLNPMIGAGMIAATAEIFIRKPQVEDFGRLRSDAATLKGWWHNQVTRILLIFLLSS
                     FGSALGTYIAGFRIFERLSSG"
     gene            3036..4886
                     /locus_tag="PK522_04405"
     CDS             3036..4886
                     /locus_tag="PK522_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC41006.1"
                     /translation="MLNFFERLINPFPPEHPTQPPNGLFPFCKHYIRGIERYLVLMAI
                     LTIAVAVGEAMLYGVLGTIIDWLSEKDPSRFLQEEYYSLIGLSIFILIVMPMLVIAHS
                     LVMYQTLMGNFPMIVRWQAHRYLISQSYAFFQNEFSGRIATKVMQTALAVRETVMKTI
                     DVLLFVSVYFATTLFLIANADFRLCLPLFFWLSIYLCILYYFIPKLRDISKLQADARS
                     VMTGRIVDSYTNILTLKLFSHTHRESEYVKSSMVNFLGTVHPQMRLVTWLHSFVWSNN
                     MLLVFCIGGLGIYLWINGNITIGAIAVALSLAIRLTGMSHWVMWEISALFENIGIVQD
                     GIDTLSKPHTVTDINDAPLLTVSSGSIHFKQISFSYQSDTQNKIIHQPIFRNFDLTIA
                     AGEKVGIVGPSGAGKSTLVNLLLRFYDVQQGNIYIDEQDISLVAQESLRTHIAIVTQD
                     TSLLHRSIRDNILFGKPDATEAQMIEAAKLAHAHEFITTLVDSKGRRGYDAFVGERGV
                     NLSGGQRQRIAIARVLLKNAPILILDEATSALDSEIEASIQENLYQLMQGKTVIAIAH
                     RLSTIAAMDRLIVMDKGQIIEQGSHAELLSHNQLYATLWNHQSGGFLGHL"
     gene            complement(4897..6135)
                     /locus_tag="PK522_04410"
     CDS             complement(4897..6135)
                     /locus_tag="PK522_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNC41007.1"
                     /translation="MLSRASLNSQIVVGVLLGVLIGAWLTTLDQTTQLAQQSLYFASL
                     ASTLFIDLLKMVLVPLVFSSIVVGVANLQTHHQINRVWVITLAYFLLTMMLAVLLGLF
                     VTNSLRPGEELQIAMFQDMMQASEVKQMSWQAFFAHFLHSLFLNPLAAMAQGQILAVV
                     VFALLFGVALVVGGDRYTNILNLMKELLELILMLVNWIMRLAPFGIMALLIQLIVSQD
                     IELLMTLIQFVAVVIGTTLFHGLIILPLILYLTTGTTPLKLWKGSREALITALATSSS
                     AATLPITLRCVEQYLHVKPDVARFVLPVGATMNMDGTALYEAIAALFIANLAGIDLNL
                     TQQLIIFFTAMFAAIGAPGIPSAGMITMVMVLQAVGLPVEAIAILLPIDRLLDTFRTM
                     VNVEGDIVGSMVVQHWVDKT"
     gene            complement(6178..6594)
                     /locus_tag="PK522_04415"
     CDS             complement(6178..6594)
                     /locus_tag="PK522_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNC41008.1"
                     /translation="MNPDTLSFWRRTLCLIYDFLLFLAIWFIASLIFIVIVQDTSFAY
                     FRPIYQFYSLSIIGIYFIWFWTHGGQTLAMQTWKLRVVAQDGKALTLRQAITRYLFAV
                     VGITSFGLGIIWALFDRDRKFLHDRLAGTRIIRVEQ"
     gene            6845..7555
                     /locus_tag="PK522_04420"
     CDS             6845..7555
                     /locus_tag="PK522_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNC41009.1"
                     /translation="MTGAKNKPLITSLSFNSLLLVLMVHAGLLYAMWHHRFPQPSLQT
                     VTLFAELISPPQKVEPANPTKVDLKAEPKPTPPEKPKVTKKIQPKQQQVAAHAPAKPK
                     EATIAPPVVNEPIKETDSSPPQEVKPAVASRPQMSADPITLSSELSVSCPELSAPDYP
                     AISRRLGEEGKLMLQVELDESGRISNTRIINSSGYSRLDNAALAAVKTWRCKPAVRDG
                     QPVKAIALQPFNFVIQGN"
     gene            7560..8321
                     /locus_tag="PK522_04425"
     CDS             7560..8321
                     /locus_tag="PK522_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNC41010.1"
                     /translation="MENGLGFSHFLAQIDQIGTSVLILLLSLSVASWYLIISKSITNL
                     IATKRANLFLENFWKLNSLQELQLNIKNLPNDNAFSELAKQAIETTKNSQRVGLEKIA
                     TSGTNELLTRTLRNGLEQEATRIEGGLTIIASAGSAAPYIGLFGTVWGIYHALIQIGL
                     SGQGTLDKVAGPVGEALIMTALGLAVAIPAVLAYNAFTRRNRIWMSKLDAFAHDLYTI
                     ITVDNQSNKQPEAITQAQPRSFSAAVNTPVFEVKQ"
     gene            8321..8740
                     /locus_tag="PK522_04430"
     CDS             8321..8740
                     /locus_tag="PK522_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNC41011.1"
                     /translation="MSFLNQERYQSQSTMSEINVVPLVDVMLVLLIIFIITAPLLTHS
                     VKVDLPKAASNPNITQPDHIEFAIRADGSFFWNGEAIHFYQLSQRFTEAAKLSQTTEL
                     HIRADKHAHYEYVARVMSIAAKSGLSKIGFITDPTIE"
     gene            8829..9338
                     /locus_tag="PK522_04435"
     CDS             8829..9338
                     /locus_tag="PK522_04435"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNC41012.1"
                     /translation="MAREKVELTIPTLIILLSLITGTLIYFIPRPIITQIYENYAENY
                     QVIEVNPRVFIYLDTDSSITIRRNEPIQIELIRGGASFDIEEAKSKILDQLEIIIGDA
                     RITNKGTRFNIKIYDKRSEITITDGQIELHLPSQKYLIDSGRKIEIDNNQIITDNIIN
                     RTEVPPWLK"
     gene            complement(9363..10223)
                     /locus_tag="PK522_04440"
     CDS             complement(9363..10223)
                     /locus_tag="PK522_04440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNC41013.1"
                     /translation="MGQKGDIASFDTHLRILTRNFNILPLSKAVNMLKSNTLPSRAVS
                     ITFDDGYADNAEIALPILKKYSTVATFFVSAGVIDGGRMWNDTIIESIRHAQGDILDL
                     SQLNMGSYPIKSLTERQKVLITLIEKLKYMPQEQRQEFVDKLSALIAAKLPNNLMMTS
                     DQVRELHREGMEIGAHTINHPILARIENPTAYNEIAEGKKMLEEIIKAPVNLFAYPNG
                     KPNKDYLLDHVKMVKEIGFDAAVSTAWGAAQAGNDIYQLPRFTPWDLNEGFFVLRMMR
                     NMFNEIEVAH"
     gene            complement(10364..11128)
                     /locus_tag="PK522_04445"
     CDS             complement(10364..11128)
                     /locus_tag="PK522_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC41014.1"
                     /translation="MPKLPVRHQFEEKWRKLFEKFATHAQDDAGIAGWSVSGLETRLR
                     YFVQVWRKENNKLQKGVWLDLGCGAGTYSKFLVDQGMYVIGLDYSFPTLKKAQLRNYS
                     SIEWCVADATKIPLRTGIFDGAICFGVTQALENSDTVIAELASATKVGGKIWVDALNR
                     WCIVHFIESFLRRLQGKPMHLRYETPANIINLMRKNNLSNIQLFWLPMLPSKLARFQW
                     VLETPFLKWVFRNVPLMGSSLCHAFIITAERNYPSD"
     gene            complement(11097..12431)
                     /locus_tag="PK522_04450"
     CDS             complement(11097..12431)
                     /locus_tag="PK522_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HNC41015.1"
                     /translation="MRDILVTIIFFGALPLVFKKPQIGVYLWAWIGYMNPHRLGWGFA
                     YNFPFGIIAAAAIILSLVIARKNITYFWSPIFSLLIVLNIWFLITTFFAIQPDISWTQ
                     WEKVIKIQLIALMIPWIINDKEKIHNFIWVIALSIGYYGIKGGVFTLTTGGSQHVLGP
                     AGSFISGNTEIGLALVSVFPLFWYLYLNTIQPWLRSGLVFAMILIPVAILGTQSRGAL
                     LAIVAIGFFLWLKSRKKLAPFIVIILFAPLLYNFMPDSWHQRMGTISSTGEERDSSAK
                     GRLLAWEFATKLALARPLGGGFECFTKENYLTYAPDIVAEGGRYQDAHSIYFELIGEH
                     GFIGLIIYLSLLVMGWKTASTIIKISKTSLSNKWAYDLASMIQVSIVGYAVGGAFLGI
                     SYFDLLYHYFVILAVLLKIVRQSVTENSQSSTTLDHEHLILKNAKITSKASI"
     gene            complement(12495..14231)
                     /locus_tag="PK522_04455"
     CDS             complement(12495..14231)
                     /locus_tag="PK522_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNC41016.1"
                     /translation="MTIFAGILARYSNSSIPNSIIEALQGAISRHPDDKNALIEYKNN
                     NIYFAKIDIGALGELGHFTDSQLSGFIAGNPILQQNSGNVYSRLESLKLLASDISKDN
                     LEALRNCRGTYCAAIYDHENGKLFLVTDKIGVRPVYFWVHSDFVIFASALRILESLRH
                     HQKEIDLKGITEIACFGYPLSDRTPYKDIFSLYAGEIVCFDTNKIERKKYWDWNKLSS
                     KKAEDLHLAKQLHATFIESIKIRLKDQKIVAAFLSGGLDSRAIVAALRELNIDVLTAN
                     FAQQGSQDQVFGQLAADLLGTHHSHLPRGKTTIGDSYHKLSTINWINSKEFLSWNPIK
                     PRVIWSGDGGSMGLGHIYQNSEIIDAARKKDLTTAKQLFSSYNRWGIFPKLMKRSLAL
                     SAQQLLADGIQSELDSIHPEDLGRQFYLFLLLNDQRRHMVEHFENIDLDRIEFELPFF
                     DANFIAEIVQQPVDRFLRHIFYLDWLGQFDSKVLEIPWQAYPGHVPCPLPKPENLEYQ
                     WESTQEDKKQAIDLALQFADSFSQNQEFMTNYINKFYFELWKLLLQWGKSNQSYLIRF
                     PAVLYRYWVKVQ"
     gene            complement(14370..15773)
                     /locus_tag="PK522_04460"
     CDS             complement(14370..15773)
                     /locus_tag="PK522_04460"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HNC41017.1"
                     /translation="MMMRLYTLNGLPIPEDWWGRYHKVVNFYDTLQRPDRTLPMIGDT
                     RSIADWFGPPKTFPLEDSAAGPLRREANKSEYAGLSLYPGAGYAIWWYPTDQSINPLS
                     KSQLVTTWSYFPGLGHKLADELSILIWANERNWITNVGYWPYGHPLRLKAESWDGSNA
                     PHLIDENKSSMRTARATKFAANDEIFYIKMIRNGPENFVVQREVIQIEKNIWIVLDKF
                     NNTIPRNTRTNWTFFPDLILQKGDEINSFVATDVQKNKSMISVIKTSSNNEVNILSGN
                     IDPFAGWVVIDETPMPANTITVHSSTEDDWQLAVFILNDSTRYPNIVIPTVSFFNLTH
                     DDQWEATITNYRDINFSLSRTKEQIHLVTKDKLNHSESILNFIDIPYPKDEISAINQL
                     VQFAYEKSTRRVPLISYRVKISYLLLALFVLQEFCLLVARKYFIAITYYRVQLAILIF
                     WLGAGAGLTEYYLVAGK"
BASE COUNT         4962 a         3156 c         2967 g         4715 t
ORIGIN      
        1 aagcggcatg ctgtaacgcc tagcaaatga ttcatcggag ataaaactag acagagtaaa
       61 tttgctctga taacagaccc ttaattgcgt ataatgtata ttatgttaaa taaaattatt
      121 ttcgaaataa atagtgctgt aaagcagata acattcccct ttctttatca gacatcacaa
      181 gttcttttta tattaatata gctccccata tattaatcat tcattttcgc tatctaatag
      241 ataaggttta cattcgtgtt tataagtgtt ttggatctct ttaaagttgg aattggccct
      301 tcaagttctc ataccatggg accgatgcgg gctgctcatg atttcctatc gcgtattgat
      361 gaaatcagct cctcattctc acgtcaatcg gtttatagtg ttcgctgtat tttgaaaggg
      421 tccttggctt atacgggtaa aggtcatgca accgatcgtg cagttacgct tggtcttcat
      481 aactatctac ctgaggagct tgcgaaagaa gacgtcaatg cgctagtaac gcggcttgaa
      541 ctggaagata cgctcgtact gaagaatggg gtcaaactta aatttaaacc tgatgaggac
      601 attgtctttg ataaaggcga accgcttcca cagcatccaa atggaatgat ttttaccgtc
      661 gttgatgaaa ataagaaaat ttggcttacc gaaacttatt tctcgattgg aggtggattc
      721 atcaatacct tagcagaaat tgatcaactc aatgcgcccc ttaaaatgga aactgcggca
      781 tcatgcccct atccatttga ttcagcgcga agtatgctaa ggatgtctcg tgaaagcact
      841 ctttccattg cagaaatgaa aaaggccaat gagttacaaa agataactgc tgatgaactc
      901 aatcaggggt tagataaaat ttggtcggca atgcagcaat gcgttgaaaa tggccttaag
      961 gcagaaggcc gactgcccgg tggacttaaa gtagaacggc gtgcaaaaaa gctatataac
     1021 caattaaatc aagacccaat caaagccacg ctgaatgact ggctatgtgc ttatgcgatg
     1081 gcggttaatg aagaaaatgc ggcgggtcac ttagttgtaa ccgccccaac gaatggagca
     1141 gctggtgtca tccctgccgt actgtattac atgacacgcc atgaaaatgg cagtattgag
     1201 aatgtaagag attttctatt aactgccgca gccattggag gcattattaa acatcgcagc
     1261 tcgatttcag gtgctgaagt cggttgtcaa ggagaagtag gttctgcagc agcaatggca
     1321 gcagcaggat tgtgtgcagc tcaaggtgga acagcacaac aaatcgagaa tgccgctgag
     1381 atcgcacttg aacatcattt aggtatgacc tgtgatccgg tcggtggttt ggtacaagtt
     1441 ccctgtatcg agcgtaatgg gtttggtgct attaaggccc atacagcagc tgcccttgca
     1501 aaacggggca ctggagaaca tttcatgcct ttagatagct gtattgctgc aatgaagcaa
     1561 actggtttag aaatgtcgca caaatataaa gaaacctcat tgggtggctt ggcagtgagt
     1621 gttacagaat gctagctagc tatttttaca aactgactac tcataccaac agtcattcat
     1681 aatttcaacc ctttaatttt tccgaccatc aattaggttg ttcatgtctg aagcaataca
     1741 acaaaataat gaatctcgtt ctaaaacatt acctgccatt gaacctctta ctacacttga
     1801 atacggtggc aacaaaataa cgattttagg cacagcacat gtttcccaag caagtgcaga
     1861 taaagttaag gaattaattg ccactggaca atatgatgcg gttgcagtgg agttatgccc
     1921 tagtcgatac aaagcgatcg ttaatcctga tgcattagcg aaaatggatt tactccaagt
     1981 gatcaaaaaa ggccaagcca gcatggttgc tgcaagccta gctttagggg ccttccagca
     2041 aagaatggcg gaacaactag gtgttgaacc tggatcggag atgagaaccg ccattaaaga
     2101 tgcaactgat gctaaattac cggtattgtt gatcgacaga gaaattggta caacattaaa
     2161 gcgcatttac tataacgtac cctggtggag aagattcgag ttatatagcg gattactcgc
     2221 aagtattatc acgcgtgaat ctgtcagttc agaagaaatt gagaaattga aagaaggtga
     2281 tgttttagaa agcgctttct tgcaattttc ccaagatcaa aaggatttat ttaaaccgtt
     2341 gatcagcgaa agagacgagt atatgtcagc gcgcttgatc aatgagtgca aggaaaataa
     2401 ttataaacat acccttgcca tagttggtgc tgggcatcta aaaggcatca aagaactctt
     2461 aatgaatcgt agcatctcca atacgaatca aaaacttgag gaacttgata cgattccgaa
     2521 cacttcttcc tggttcaaat acctaccctg gataattgtt attttaattt taactggctt
     2581 tgcttttggt tttgatcgta gctcagatat cggcatggca atgatcatcg attgggtctt
     2641 cattaatggc ggattagctg ccctcggtgc cattatagcc ctggcacacc cattgactat
     2701 cctaactgct tttttagctg caccgattac ctcactcaat ccaatgatcg gtgcgggcat
     2761 gattgctgct actgcagaaa tattcatacg taaaccccaa gtagaagatt ttggtcgctt
     2821 aagaagcgat gctgctacct taaagggatg gtggcataac caagtgactc gaatattact
     2881 tatttttctt ctttcgtctt ttggctcagc attaggtacc tatattgcag gcttcaggat
     2941 atttgaaagg ctatcaagtg gttaatagca taagataact cgaatccttc agatgcgggt
     3001 atcttcctac ttctttgcca gcttgggtaa atactatgct taattttttt gagcgtctaa
     3061 tcaacccatt cccgcctgag caccctactc aaccgcccaa tgggcttttc ccattttgca
     3121 agcactatat ccgaggtatt gaacgctatc tcgtgttgat ggctatcctg accatcgcag
     3181 ttgcagtcgg tgaagcaatg ctctatggcg tgttaggaac aatcatcgat tggttatcag
     3241 aaaaggaccc tagtcgtttt ttgcaagaag aatattactc acttattggg ttgtcgattt
     3301 ttatcctgat agtaatgcct atgttagtga tcgctcactc actggttatg tatcagaccc
     3361 tgatgggtaa ttttccaatg attgttcgct ggcaggcgca tcgttactta atcagccaga
     3421 gctatgcgtt ttttcagaat gaattcagtg gtcgtattgc aaccaaagtc atgcaaactg
     3481 cgctggccgt tcgtgagacc gtcatgaaaa caattgatgt gctgcttttc gtgtcagttt
     3541 actttgctac aacgcttttt ttaattgcaa atgctgactt tcgcttatgc ctacccttat
     3601 ttttctggct gagcatttac ctctgcattc tttattattt tattcctaaa ttaagggata
     3661 tttctaagct tcaagcggat gcacgatccg tcatgacggg acgaatcgtc gacagctata
     3721 ccaatatact cacgttaaag ttattttccc atacgcatcg tgaatcagaa tatgttaaat
     3781 ctagtatggt gaattttctt gggaccgttc atccacaaat gcgcctagtg acttggctcc
     3841 attctttcgt ttggagtaac aacatgttgc tggtgttttg tattggcgga ttaggcattt
     3901 atttatggat caatgggaat attacgattg gtgcaatcgc ggttgcactg agcttggcca
     3961 ttcgattaac gggtatgtcg cactgggtca tgtgggaaat cagtgcgtta tttgaaaata
     4021 ttggtatcgt gcaagatggt attgatacgc tgtccaagcc acatactgtg acggatataa
     4081 acgatgctcc cttattaaca gtcagctctg gatccattca ttttaagcaa atcagctttt
     4141 cgtatcagtc tgacactcaa aataaaatca ttcatcagcc catattcagg aattttgatc
     4201 taacgattgc agcgggagaa aaagttggca ttgttggtcc atctggagct ggaaaatcaa
     4261 cattagttaa tttattactt cgtttttatg atgttcagca aggcaatatc tacatcgatg
     4321 aacaagatat cagcttagtt gctcaggaaa gtttacgtac tcatatcgcg atcgtaacgc
     4381 aagatacttc cctcctccat cgatcgatcc gtgacaatat tttatttggt aaaccggatg
     4441 caaccgaggc ccaaatgata gaggcggcta aattagccca tgcccatgaa ttcataacta
     4501 cgctggtcga tagcaagggc cgcagaggtt atgatgcgtt tgtcggagag cgtggtgtta
     4561 atttatcggg cggccaacga cagcgcattg ctatcgcacg cgtattactt aaaaatgccc
     4621 caattcttat cttagatgaa gctacctctg ccttagattc agaaattgag gcgagcattc
     4681 aggaaaattt ataccaatta atgcaaggca aaacagtcat tgcgattgca catcgattgt
     4741 ctaccattgc cgcaatggat cgactgattg tgatggataa aggtcagatt atcgagcaag
     4801 gtagtcatgc cgagctacta tcccataacc aactatacgc aaccctttgg aatcatcaat
     4861 ctggaggatt tctgggtcat ttataggaag tattgattaa gtcttatcca cccaatgttg
     4921 tacaaccata cttccaacaa tatccccttc aacattaacc atcgtccgaa atgtgtctaa
     4981 taatcggtcg attggcaata aaatcgcgat tgcctcaact ggtaacccta cagcctgaag
     5041 caccattacc atagtgatca ttcctgcact gggtatacca ggagcgccaa tcgcggcgaa
     5101 cattgcagta aaaaaaatga tcaactgctg agtaagattt aaatcaatac cggcaagatt
     5161 tgcaataaat aacgctgcta tcgcttcata cagcgcggta ccatccatgt tcatggtcgc
     5221 gcctactggc aagacaaatc tcgctacatc aggttttaca tggagatatt gttccacaca
     5281 gcgtaatgtt atgggtaatg ttgcagcact agaactcgtt gctagtgctg taattagtgc
     5341 ctcacgcgat cctttccaca atttaagcgg tgtggttcct gtcgtcaaat aaaggatcaa
     5401 tggtaaaatg atcaagccat gaaaaagcgt tgtgccaata acgacagcca caaattgaat
     5461 gagcgtcatt aacaattcaa tatcttggga aacgattaat tgaatcaata aagccataat
     5521 tccaaatggc gcaagtcgca taatccaatt cacaagcatt aagataagct ctaaaagctc
     5581 tttcattaaa ttcagaatgt ttgtataacg atctcccccc accaccagcg caacaccaaa
     5641 tagtaatgca aaaacgacaa cggccaggat ttgaccttgc gccatggctg ccaatgggtt
     5701 gagaaatagc gaatgcagaa aatgagcgaa aaacgcttgc caagacattt gcttaacttc
     5761 ggaggcttgc atcatatcct gaaacatagc aatttgtagt tcttcaccag gccgcaagct
     5821 attggtaaca aatagcccaa gtaaaactgc aagcatcatg gttaatagaa aataggctag
     5881 cgtaataacc catacgcgat tgatttggtg atgggtttgt aaattagcca cgcccactac
     5941 aatcgacgaa aacaccagtg gaacaagcac cattttgagc aaatcaataa ataaagtgct
     6001 tgctaagcta gcaaagtaca agctttgctg cgctaattgt gttgtttgat ctaatgtggt
     6061 taaccaagcc ccgattaaaa cacctaataa cacaccaact acaatctgac tattgagtga
     6121 agctcttgat agcatggatg atatttttgt attaaataca ggatgattga gcctcgttta
     6181 ttgctcgacc ctgatgatac gagttcctgc tagtcgatca tgtagaaact ttcggtcacg
     6241 atcaaaaagt gcccagatga ttccaagtcc aaacgaagtt ataccgacta ctgcaaatag
     6301 gtaccgagta atcgcttgtc gcaaagtaag tgctttacca tcttgtgcta caacacgtaa
     6361 tttccaggtt tgcattgcaa gtgtttgtcc tccatgtgtc caaaaccata tgaaatagat
     6421 tccaataatt gacagtgaat agaattggta tatggggcgg aaataggcaa agctggtatc
     6481 ttgtacaatt acaataaaaa tcaggcttgc aataaaccaa attgctaaaa acaataaaaa
     6541 atcgtaaatc aggcaaagcg tgcgtcgcca aaatgataaa gtatcaggat tcattgaata
     6601 agttaaatta aacaaaattg gtaatttaca tctaggatgt taatccgaaa agaatcttag
     6661 taacaaattc atactacact aatttatcag cctcttttgt aaagtctgtc attactcaat
     6721 atcacaaaaa gaagctacaa ggagatcaca agtgattgat aattattatt gtttgcgtta
     6781 gtatttgtta ctaatcaaaa taataatttt acccaatcat tgccctaaaa aaatagctca
     6841 ttgaatgact ggtgcaaaaa ataaacccct tatcacttca ctatcgttta acagtttgtt
     6901 actcgtgttg atggttcatg caggattact ctatgcaatg tggcaccatc gcttccccca
     6961 gccttcatta caaactgtta cgttatttgc agaattaatt tcaccccctc aaaaagttga
     7021 gccagcaaac cctacaaaag ttgatcttaa agctgagcct aagcctactc cacctgaaaa
     7081 acctaaagtt actaaaaaaa ttcaacctaa acagcaacaa gtagcggcac atgcgcctgc
     7141 aaagcctaaa gaagcaacga tagcaccacc cgtagtcaat gagcccatta aagaaactga
     7201 ttcatctcca cctcaagaag tcaaacctgc agtggcatct cgtccgcaaa tgtcagctga
     7261 tccgataacg ctatcctcag aattatcagt ttcgtgccct gaactcagcg cgccggatta
     7321 cccggctatt tcccgtcggc taggagaaga aggtaagctc atgctgcaag ttgaattgga
     7381 tgaatcagga cgaattagta atacccgcat cattaatagt agtggttact ctcgcctcga
     7441 taatgctgcg ctcgctgcag taaaaacttg gcgctgtaaa cccgctgtta gggacggtca
     7501 acccgtcaag gctatcgctt tacaaccttt taattttgtt attcaaggaa attaatttaa
     7561 tggaaaatgg attaggattt tctcattttc ttgctcagat tgatcaaatc ggaacaagtg
     7621 tactcatctt actgctatcg ctttctgtcg caagttggta tttgattatt tcaaaaagta
     7681 tcactaactt aattgccacg aagcgtgcta atttatttct agaaaacttt tggaaattaa
     7741 attccctgca agagctgcaa ttaaacataa aaaacttacc gaatgataat gctttctcag
     7801 aattagcaaa gcaagcgatt gaaaccacca aaaattctca gagagtagga ttggagaaga
     7861 tagctacgag tggaaccaac gaattactca cgcgtacatt acgaaatgga ttggaacaag
     7921 aagccactcg tatcgagggg gggttaacca ttattgcttc ggctggttca gctgcacctt
     7981 atatcggatt atttggtacg gtatggggca tctatcatgc attaattcaa attggactct
     8041 ctgggcaagg tactttagat aaagtagccg gacctgtcgg tgaagcgcta attatgactg
     8101 ctttagggct ggctgttgca attcctgcag tattagccta caacgccttt actcggcgaa
     8161 atcgaatatg gatgtcgaaa ttggatgctt ttgcacacga tctctatacg attattactg
     8221 ttgataatca atctaataag cagcctgaag caataacaca agctcaaccc cgttcttttt
     8281 cagcagcagt taatacacct gtgtttgagg taaaacagta atgtcatttc ttaatcaaga
     8341 acggtatcag agtcaatcga caatgtcaga aatcaatgtt gtcccattgg ttgacgtgat
     8401 gctagtgttg ctcatcattt ttatcattac tgcgccttta cttacgcatt cagtcaaagt
     8461 tgatctacct aaagcagcga gtaatcccaa tattactcaa cctgatcaca tagagtttgc
     8521 gattcgagct gacggtagct ttttctggaa tggtgaagca attcatttct accagcttag
     8581 tcaacgattt accgaagcag caaaactttc ccaaacaacg gaattgcata tccgagccga
     8641 taaacatgcg cattatgagt atgtcgctcg cgttatgagt atcgcagcca agtccggttt
     8701 atctaaaatt ggatttatta ctgatccaac catagagtaa ttgttgtata gcgttatacg
     8761 ctctttagcc atagccataa gagacttact ctatttttgg aatttgagaa aaagtattct
     8821 tttttcttat ggctagagaa aaagtagaac tgactatacc tacgctaatt attttattgt
     8881 cgcttattac tggcacatta atttatttca ttcctcgccc aatcattacg caaatttatg
     8941 aaaattacgc tgaaaactat caagtaattg aagtaaatcc tcgagttttt atatatttag
     9001 atacggatag ttcgatcaca atacgaagaa acgagccaat acaaatagaa ttaatacgtg
     9061 gtggagcttc ttttgatata gaagaagcaa aatcaaaaat actagatcag ttagaaatta
     9121 tcattgggga tgctcgcatt actaataagg gcactcggtt taatatcaaa atatatgata
     9181 agcgtagcga gatcacaatt accgatggac aaattgaatt acacttgcca tcccagaaat
     9241 acctcattga ttcaggaagg aaaattgaaa ttgataataa tcagattatt actgataata
     9301 ttatcaatcg aactgaagtg cctccttggt taaaataatt atacttgtaa ggtttagtac
     9361 tgtcagtgag ctacctcgat ttcgttgaac atattccgca tcatgcgtaa tacaaagaaa
     9421 ccctcattca gatcccaagg cgtaaatctt ggcaattgat agatatcgtt acctgcttga
     9481 gcagcacccc aagctgttga cactgctgcg tcaaaaccta tttcttttac cattttgaca
     9541 tgatcaagca aatagtcttt attaggtttt ccatttggat acgcaaataa attgactggt
     9601 gccttgatta tttcttcaag catttttttc ccttcagcga tttcattata agcggttgga
     9661 ttttcaatcc tggccagaat cggatggttt atagtatgtg caccaatttc cattccctca
     9721 cgatgtagtt ctctaacttg atctgatgtc atcattagat tgtttggtaa ttttgccgcg
     9781 atgagtgcgc tcaatttatc tacaaattct tgtctttgct cttgaggcat atattttaac
     9841 ttctcaatta atgtgattaa tactttctga cgttcagtca gggattttat tggataactt
     9901 cccatattga gctggctaag gtctaatata tctccttgag catggcgaat tgattctata
     9961 atcgtatcat tccacattct tccaccatca atcacgccag ccgaaacaaa aaaagtagct
    10021 actgtagaat atttctttaa aattggtaat gcaatttctg cattatctgc atatccatca
    10081 tcgaatgtaa tactgactgc acggctgggt aaagtattac ttttaagcat atttactgct
    10141 ttggataatg gaagaatatt aaaatttcta gtcaaaattc tcagatgagt atcaaaactt
    10201 gcaatatctc ctttctgacc cacaagaaga tccggttgaa gtaaaacacg atgataaata
    10261 acaatcgata accgagctct tttacctgac ggtgagaaaa tcaatggtag gagtttcatg
    10321 ggagattcat tttaatctta atttttttct agtaatttta ctatcagtcg cttgggtagt
    10381 ttctctcagc agtaataata aatgcgtgac ataacgaact tcccattaat ggaacattcc
    10441 gaaacaccca ttttaaaaaa ggagtttcta gtacccactg gaaacgagcc aattttgaag
    10501 gaagcattgg taaccaaaat aactgaatat tacttagatt attttttctc attaaattga
    10561 taatattagc aggagtttca taacgaaggt gcatgggttt tccttgtagt ctacgcagaa
    10621 aactttcaat aaaatgaaca atgcaccatc gatttaatgc atcaacccag atttttccac
    10681 ctacctttgt agctgaagct agttcagcaa tcacagtgtc agagttttca agtgcttgtg
    10741 ttacgccaaa acaaatagca ccatcaaaaa taccagtgcg taatggaatt ttagttgcat
    10801 ctgcaacaca ccattctatt gaggaataat ttcttaattg agctttcttt aaagtgggga
    10861 atgaataatc tagaccgata acatacatcc cttgatctac aagaaattta gagtaagttc
    10921 ctgctccaca accaagatct agccatactc ccttttgaag tttattattt tcctttctcc
    10981 aaacttgtac aaaatagcgt aagcgtgttt ctaagccaga gacagaccac cctgcaatcc
    11041 ctgcatcatc ttgcgcgtgt gttgcaaact tttcaaacag tttgcgccac ttttcttcaa
    11101 attgatgcct tactggtaat tttggcattt tttagaatca aatgttcatg gtctaatgtg
    11161 gtgcttgatt gtgagttttc cgtaactgat tgccgcacta tttttaataa aactgccaat
    11221 ataacaaagt agtgataaag aagatcaaaa taagatattc ctaaaaaggc accgccaaca
    11281 gcataaccca caatactaac ctggatcata gaggcaagat catatgccca tttatttgat
    11341 aaggatgttt tagaaatttt tataattgta ctagctgttt tccagcccat aactaacaaa
    11401 ctcaagtaaa ttattaaacc gataaaacca tgttcaccaa ttagttcaaa ataaatacta
    11461 tgagcgtcct gatatcttcc tccctcggct acaatatcag gagcataggt taaataattt
    11521 tctttagtaa aacattcgaa tcctccaccc aatgggcgtg ctaatgcaag ttttgtagca
    11581 aattcccaag ctaagagccg tcctttagca gaactatccc tttcttctcc tgtactgcta
    11641 atagttccca ttcgctgatg ccatgaatct ggcatgaaat tatatagaag tggtgcaaac
    11701 aaaataatta caataaatgg agcaagtttt ttccggcttt ttaaccaaag gaaaaaacca
    11761 attgctacaa ttgctaataa tgcaccgcgt gattgagttc ctagaatagc cactggtatt
    11821 agtatcatag cgaaaactag tcctgatcgg agccatggct gaattgtatt gaggtacaaa
    11881 taccaaaata atgggaatac tgaaaccaat gccaagccaa tttccgtatt accagatata
    11941 aaactacctg ctggccctag tacatgctga ctaccacctg ttgtaagcgt aaatacaccg
    12001 cctttgatac catagtaacc tattgataaa gctataaccc aaataaaatt atgaattttc
    12061 tctttatcat taattatcca aggtatcatc aaagcaatta attgtatttt gataaccttt
    12121 tcccactgag tccaagaaat atccggttgt atagcaaaga aagtggtaat cagaaaccag
    12181 atatttagaa caattaagag gctaaaaatt ggtgaccaaa aataagtaat atttttacga
    12241 gcaataacaa gcgataaaat aatggcagct gccgcaatga ttccaaaagg gaaattatat
    12301 gcaaaccccc accctaatcg atgtgggttc atataaccaa tccaagccca taaataaacc
    12361 ccgatctgag gtttcttaaa aacaaggggc aaggctccaa aaaaaataat cgtaactaat
    12421 atatccctca tgaatcaatc attaatctat tgaagcaatt tgatagaaaa ctttatctac
    12481 tacagctagt agagctattg gaccttaacc caatatcgat ataaaaccgc aggaaatcga
    12541 atgagatatg attgattaga tttgccccat tgcaataata atttccataa ttcaaagtaa
    12601 aatttgttaa tgtaattcgt cataaactct tgattttggc taaaggaatc tgcgaattgc
    12661 aaggctagat caatagcttg ttttttatcc tcctgggttg actcccattg atattctaga
    12721 ttctctggtt taggtaaagg acaaggaaca tgacctggat aagcttgcca tggtatttcc
    12781 agaaccttag aatcaaattg acctagccag tcgagataaa aaatatgtcg aagaaatcga
    12841 tcaactggtt gctgtactat ttcagctata aaattagcat caaaaaaagg taattcaaat
    12901 tcgatgcggt ctaaatcgat attctcaaag tgttcaacca tatggcgacg ctgatcattg
    12961 agaagtaaaa acaagtagaa ctgacgacct agatcttctg ggtgaatcga atctaattca
    13021 gattgaatgc cgtctgcaag taactgttga gcagataacg ccaaagagcg cttcattaat
    13081 ttaggaaata ttccccagcg attgtaggaa gaaaaaagtt gctttgcagt ggttagatct
    13141 ttttttcgag cagcatcaat tatttctgaa ttctggtaaa tatgaccaag gcccatacta
    13201 ccgccatcac cagaccagat tactcttggt tttatagggt tccatgaaag aaattctttg
    13261 gagtttatcc aattaatcgt agaaagttta tgatatgaat ccccaatagt tgttttacct
    13321 ctaggaagat ggctatgatg tgtacccaac aaatcagctg ccaattgacc aaatacctga
    13381 tcctgacttc cctgttgagc aaaattggcc gtcaatacat ctatattaag ttctcttagc
    13441 gctgcaacga ttgcacgtga atccaggccg ccacttaaaa aggccgcaac gattttctga
    13501 tctttcaatc gaattttaat cgactcgatg aaagtcgcat gaagctgttt agcaagatgt
    13561 aaatcttctg ctttttttga ggataatttg ttccaatccc aatatttttt tctctcaatt
    13621 ttattggtat caaaacacac aatctcaccc gcataaagtg agaaaatatc tttatatggt
    13681 gttcgatctg ataatggata accaaagcaa gcgatttcag taatgccttt tagatcgatt
    13741 tctttttgat gatgtcttaa agactcgaga atccgtagcg cagatgcaaa aataacaaaa
    13801 tcggaatgta cccagaaata aactggcctc acaccaattt tgtcagttac gagaaagagt
    13861 ttaccattct catggtcgta gatagccgcg cagtatgttc ccctgcagtt acgaagtgct
    13921 tccaaattat ccttactgat atcagaagca agaagcttca agctctctaa tcgtgaataa
    13981 acattaccag aattttgctg gagaattgga tttcctgcga taaatccgga tagctgagaa
    14041 tctgtgaaat ggccaagctc acctaatgca ccaatatcaa ttttagcaaa ataaatatta
    14101 ttatttttat attcaatcaa tgcattttta tcatcaggat gacgtgaaat tgctccctgc
    14161 aacgcttcaa ttattgaatt tggaatacta gaattagagt agcgcgctaa aatccctgca
    14221 aaaatagtca ttaaaaatcc aattttttag agtgaaatat aaaaaattaa ccggatagta
    14281 acactgatat gtaattttat tatttttaaa aagaaacctt attcaaggta tttgttcgca
    14341 tctggcatca aggtaacttg tctaatatat tatttaccag caactaaata atattccgtc
    14401 aatccagctc ctgctcccag ccaaaagatt agaatagcta attggactct ataataggtt
    14461 atagcaataa aatatttacg agccaccaac aggcagaatt cctgaagaac aaacaaagct
    14521 agcaataggt aacttatttt tactcgataa gagatgagtg gtactctgcg agtgcttttc
    14581 tcataagcaa attgtaccaa ctgattaatt gctgagatct catccttagg gtatgggata
    14641 tcgataaaat taagtataga ttctgaatga tttaatttat cttttgttac caagtgaatc
    14701 tgttcttttg ttcgacttag cgaaaagtta atatcacggt aattagttat agtggcttcc
    14761 cattggtcgt catgagttag attaaaaaat gaaaccgttg gaatcactat attaggataa
    14821 cgcgtgctgt cattcaggat gaaaactgct aattgccaat catcctctgt acttgaatga
    14881 actgtgattg tattagctgg cataggtgtt tcatcgatta caacccaccc agcaaatgga
    14941 tcaatgttgc cagataggat attcacttca ttatttgaac ttgttttgat gacacttatc
    15001 attgacttat ttttttgaac atctgtagct acaaaactat taatttcatc tcctttctgt
    15061 aatattaagt caggaaagaa agtccaattt gtacgtgtat tacgtggaat ggtattatta
    15121 aatttatcta atacaatcca aatatttttt tcaatttgta ttacttcacg ttgtaccaca
    15181 aaattttctg gaccatttct tatcatcttg atatagaaaa tttcatcatt agcagcaaat
    15241 ttggttgccc tggcagtacg cattgatgat ttattttcat cgattaaatg aggtgcatta
    15301 ctaccatccc aggattcggc ttttaatcga agcggatgtc cataaggcca ataacccaca
    15361 ttagtgatcc aatttctctc attcgcccag attaaaatac tcaattcatc tgcaagctta
    15421 tgtcctaaac ctggaaaata tgaccaagtt gtaacaagtt gtgatttaga taaaggattg
    15481 atactttgat ccgttgggta ccaccaaatt gcgtacccag ctcctggata taaagacaaa
    15541 ccagcatatt ccgatttatt agcctcgcga cgcaatggcc ctgcagcaga atcttccagc
    15601 ggaaatgttt tgggggggcc aaaccagtca gcaatacttc gagtatcacc gatcatcggt
    15661 aatgtgcgat cagggcgctg taatgtgtca taaaaattta caactttatg atatcgcccc
    15721 caccaatctt ctggaattgg aagcccatta agtgtataaa ggcgcatcat cattgaaaag
    15781 agcctaacac cgccgtagtt
//
LOCUS       DAOQLJ010000040        15731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1353_length_15731_cov_6.370503, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000040 DAOQLJ010000000
VERSION     DAOQLJ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 15731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15731
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1353_length_15731_cov_6.370503"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PK522_04465"
     CDS             <1..225
                     /locus_tag="PK522_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41018.1"
                     /translation="FSWGNFWQYNLSSYDIVYAYLSPIPMDKLWKKVLQEMRPGSILI
                     SNSFIVPDVPPTESIAINDFNNSVLYIWKI"
     gene            complement(232..1617)
                     /gene="cysS"
                     /locus_tag="PK522_04470"
     CDS             complement(232..1617)
                     /gene="cysS"
                     /locus_tag="PK522_04470"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNC41019.1"
                     /translation="MLKIYNSLTRAKEEFIPLVPGEVKLYVCGMTVYDFCHLGHGRVL
                     VVFDMVVRWLQSIGYNVTYVRNITDIDDKIIKRAQEMNESIETLTNRFIKAMHEDANA
                     LGTVKPNFEPCATHHVGEMITLIRKLIEKDCAYVADNGDVFFSVHHFPGYGKLSGKSL
                     SDLRAGERVEIDHHKRDPLDFVLWKSVKPGEPYWQSPWGNGRPGWHIECSSMSGHFLG
                     NHFDIHGGGQDLQFPHHENEIAQSEGANEETYVNYWMHNGFVRVDNEKMSKSLGNFFT
                     VREILKNFQAEVIRFFILRAHYRSPLNYSLEHLISAKNALDRLYTALKGYETVNAGKI
                     NWDNHHAQSFKIAMNDDFNTPEAIAILFNLSSEINRSQKPQDASLLKSLANILGLLQQ
                     EPQRYLQQDLVANNKNEKFSESEINLLINQRLEAKKNQDFKEADQIRSLLAEAGIILE
                     DSSQGTTWRRQ"
     gene            1768..2343
                     /locus_tag="PK522_04475"
     CDS             1768..2343
                     /locus_tag="PK522_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC41020.1"
                     /translation="MIRLITLLLALSINLSALAAQPIVRIDTNLGPISLALYSDQAPK
                     TVENFLHYVREGFYAETIFHRVIPNFMIQGGGFDQALVQKPTYQPIINEAANGLKNEI
                     GTIAMARTSDPHSATAQFFINVANNTFLNYSSPTAQGYGYTVFGKVIEGMDVVNKIAA
                     LPTGPRGIFAKDVPKDTVVIKAIELLSDNKE"
     gene            2373..2864
                     /locus_tag="PK522_04480"
     CDS             2373..2864
                     /locus_tag="PK522_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC41021.1"
                     /translation="MVKFHTNYGVITLALYDEQAPLTVKNFLSYVSSGHYDNTVFHRV
                     IDNFMIQGGGFDSNMMQKKTLEPIQNEASNGLKNETYTIAMARTSDVHSATSQFFINV
                     KNNSFLDFTSPTTQGYGYCVFGKVIEGMEVVDEIKKVKTGNYAGHENVPLENVVIERA
                     EIV"
     gene            complement(2878..3807)
                     /locus_tag="PK522_04485"
     CDS             complement(2878..3807)
                     /locus_tag="PK522_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HNC41022.1"
                     /translation="MVTFSSSLTHEEYEFILMAVILIALISFYSFFKNWKRLRFIEDT
                     PTANLRSAHQGYVEIEGKGALIEDKPIYAPLSNHRCLWYRSQIECQEILIEKGQSRIN
                     WNVIYSNISHHSFKLTDGSTSCFVDPDSAEIQGEEKLVWYGNTEWPNQTQILESQSVL
                     HSNNNRYRYSESLILPGQSLYVLGQFSTSSAASQHSIREIMNNLILDWKKDSQHLLKR
                     FDANKDGQIDQQEWESARQIASAESQQIYQQRLLEPEHNIITKPKDKRYPFIISVYSQ
                     ASLRQKYRLTAYLSLLACLVLTGYIIWFAHTHG"
     gene            complement(3825..4367)
                     /locus_tag="PK522_04490"
     CDS             complement(3825..4367)
                     /locus_tag="PK522_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNC41023.1"
                     /translation="MLVIAVIYGIMIYNSLVDVKHSVSQAWSNIDILLKQRHDELPKL
                     VETCKQYMGFEQETLKQVIAARSQVASARDAGNLPALGAAENMLRTGLGNLFAVVEDY
                     PELKSSEKFQHLQSRITGLENSIADRREYYNEAVKINNVRIEQFPEVIIANWFKFNAR
                     DLLQFNATEKQDVDVGKLFS"
     gene            complement(4487..5158)
                     /gene="trmB"
                     /locus_tag="PK522_04495"
     CDS             complement(4487..5158)
                     /gene="trmB"
                     /locus_tag="PK522_04495"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNC41024.1"
                     /translation="MSSAISIRSYAIRNGRYTNGQRKAYETLFSDFSIPFQQEALNLN
                     TIFGRCAPKILEIGSGMGETTATIAQQSPDNDYLAIEVHTPGIGSLLKLIDLQGLSNL
                     RIIQYDAVAVLNLMIEKESLDGVHIFFPDPWPKKRHHKRRLIQATFVTLLCERIKSGG
                     YLHIATDWQDYAEQILMVLSNQTSLINNNKDYAQRPTSRPLTKFEQRGIKLGHQVWDL
                     IFTKK"
     gene            complement(5189..5983)
                     /locus_tag="PK522_04500"
     CDS             complement(5189..5983)
                     /locus_tag="PK522_04500"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNC41025.1"
                     /translation="MDDFIIAGKCYKSRLLVGTGKYKDFSETRAAIEASGSEIITVAI
                     RRTNIGQNPDEPNLLDVLPPSKFTLLPNTAGCYTAEEAIRTLRLARELLDGHSLVKLE
                     VLGDSKTLFPNIVETIKAAQTLVKENFQVMVYTSDDPIVAKQLEEVGCVAIMPLASLI
                     GSGMGILNPWNLQIMIDRATVPVLVDAGVGTASDATIAMELGCDGVLMNTAIAAAKDP
                     VMMASAMRKAVEAGREAYLAGRMEKKFYSASPSSPIDGISPLSSHK"
     gene            complement(6050..6253)
                     /gene="thiS"
                     /locus_tag="PK522_04505"
     CDS             complement(6050..6253)
                     /gene="thiS"
                     /locus_tag="PK522_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNC41026.1"
                     /translation="MIQLIINGEPQQFESSLNLQQLLENMGLQNKRIAIEHNGEIIPR
                     SRFSDYIVTEGDRLEIVVAVGGG"
     gene            6313..6386
                     /locus_tag="PK522_04510"
     tRNA            6313..6386
                     /locus_tag="PK522_04510"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6345..6347,aa:Gly,seq:ccc)
     gene            6462..7055
                     /locus_tag="PK522_04515"
     CDS             6462..7055
                     /locus_tag="PK522_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease"
                     /protein_id="HNC41027.1"
                     /translation="MKRKIRGLFVFFWIVTLLIISHAVLADNKISQPESKLIVGWVEF
                     IRLEPWGMKMRARIDTGANTSSMSARDIHQFKKGNEDWVKFVLDFKTDKGKPNRSVEI
                     ERPITRIVKIKQHQGTLQERPVVNMDICIANQVHNIEFNLIDRRGLNYPVLLGRRALE
                     NLALVDPARTFIEKADCGHIKKKKKKADTPPDSISLE"
     gene            7052..8578
                     /locus_tag="PK522_04520"
     CDS             7052..8578
                     /locus_tag="PK522_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inactive transglutaminase family protein"
                     /protein_id="HNC41028.1"
                     /translation="MRQLHLLFLTLLLCAIGLGLAAYKVDTLGLPLTASAQTEVWNVE
                     ARISFKAKPDSGVKVSLPLPFNPSGYTVLNEDFVGANYGLATEQDEGGRVALWAKRRA
                     QGKQLLFYRAVIFENEEKIADSSKIPSYPAVPDYPEHFLPVIQGLLDKSRQQSADTLS
                     FAQQVIQQLNATAPAPELKLLIEHINTSKNLAKGISWILAGARVPARVIQGLRLQDGK
                     RYSELEAWVQVHDGKQWRTLNTKDGQEGLPTNFVIWQIGDQKTFSVEGGTEISLQFSV
                     AKALRELVDVAESRAILKGSRIMEFSLYSLPVQTQNVYKVLLMIPLGAFVVVLMRNLI
                     GVKTFGTFMPVLIALAFRETELVWGLALFCLIVGLGLLLRAYVEQLKLLLIPRLASVL
                     TMVVLLMAGTSVVMHKLGLEMGLSVALFPMVIMTMTIERMSLTWEEAGPIEAFKQGAG
                     SLLVATIGYLVMNIDELQHLLFVFPELLLVVLAVTILFGRYSGYRLTELWRFRALAKR
                     "
     gene            8588..9535
                     /locus_tag="PK522_04525"
     CDS             8588..9535
                     /locus_tag="PK522_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HNC41029.1"
                     /translation="MFSTWLNLKRAGVIGMNRRNADFILRYNERSLYPLVDDKLRTKQ
                     LAIAAGIAVPQLYGVIEIEHDIVNLPGIVKDYKDFVIKPAHGSGGNGIAVIIGRINER
                     FQYNNGSLLTEEELEYHISNILSGMYSLGGQPDQALIEYRVKFDPLFESVSYRGVPDI
                     RTIVFRGVPVASMVRLPTRLSDGKANLHQGAVGAGIDLTSGVTTRAVWQNRPIDIHPD
                     TGSAISGLAIPHWNKLLTLAAQCYELVGLGYLGVDVVLDHELGPLVLELNARPGLSIQ
                     IANGKGMEKRLEYIDRLSEIPEKAQDRAALAQSLFKAEN"
     gene            9599..10639
                     /gene="glk"
                     /locus_tag="PK522_04530"
     CDS             9599..10639
                     /gene="glk"
                     /locus_tag="PK522_04530"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HNC41030.1"
                     /translation="MDSRFIIYGDIGGTKTILRLAKQQNSDLLHIREQRYASQDYQNL
                     DEIINDFLIGLPDTNAANKAISAACFAIAGPIAAQQARLTNLPWLISAADLSRKFAIP
                     QVKLINDFEATALGVEKLTQQDLEVIQIGNMNEASMRVVLGAGTGMGVAWIASIGNRY
                     IPFPTEAGHTDFAPINDLQIELLNFLAAKFGHVSIERVLSGPGLTNIFNFFQQRANHL
                     RSSSLPELSEDEGQLIIQLALEQNHVTAIESLQLFTEIYGAFAGNLALTGLCRGGVYI
                     AGGIAPRILHYLKTERFIQAFRNKGRFTGLMREIPLSVILNTQVGLLGAESEAQRLQN
                     LLVANSSNNTNT"
     gene            complement(10642..11475)
                     /locus_tag="PK522_04535"
     CDS             complement(10642..11475)
                     /locus_tag="PK522_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC41031.1"
                     /translation="MRTTLRLIILGISIYAAWAIFIFFAQSHLIYHPEIDREIVNTPD
                     QFSMPYESVVLTTSDQEKLHGWFVPAAKETTATILFLHGNAGNISHRMGYLSIFHQLG
                     YNTFIVDYRGYGNSSGTPTEEGTYQDALTAWNYLIENKTIPGSHIVLFGESLGGAIAA
                     WLASKNDPGALVLASTFTSIPDIAADLYPLLPVRLMARFHYNTSKYLQSVTCPVIVAH
                     SPEDEIVPFKHAQALFDKAPNPKQFIVLQGKHNDSFFVEQDAWVHPLNQFIRASLSAY
                     K"
     gene            11567..12115
                     /gene="ppa"
                     /locus_tag="PK522_04540"
     CDS             11567..12115
                     /gene="ppa"
                     /locus_tag="PK522_04540"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNC41032.1"
                     /translation="MNLDRVDSGRDLPNDFNVIIEIPMNSEPIKYEMDKKTGAMFVDR
                     FMSTAMRYPCNYGYIPHTLSEDKDPVDVLVISPTPLITGVVVRCRPIGLLNMTDEAGE
                     DVKLIAVPIDKLCGLYIHVQTLDDLPEFMRAQIAHFFAHYKDLERGKWVKIEGWGDVE
                     AARAEIMTGVKRYNSAKNKPKF"
     gene            complement(12157..13335)
                     /locus_tag="PK522_04545"
     CDS             complement(12157..13335)
                     /locus_tag="PK522_04545"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC41033.1"
                     /translation="MNSYDVIIIGAGAAGLMCAIEAGKRGRSVWLIDHMAKFAEKIRI
                     SGGGRCNFTNIFTQPQHYLSQNPHFCKSALAQFTPHDFINLVNKHHIAFHEKKLGQLF
                     CDHSSQQIIDMLLSESHQAKVSFALQTRVTQIEALADGYRLDTNRGIHTCRSLVIATG
                     GLSIPKIGASNFGYEVAKKFDINVIKPRPGLVPLTFDGTLLEQCKSLSGVSIDATVQF
                     NRQSFSEGLLFTHRGLSGPSILQISSYWEQGKAIRVNLIPDIDLYSVLKAKKNNQPKL
                     HIGGVLAERLPKRLAQNLCDQYVFTGAIGILSDRQLAKIAEVIQAWLIWPTGTEGYRK
                     AEVTIGGVDTNELSSRTLEAKKRPGLYFIGEVVDVTGHLGGFNFQWAWSSGFAAGQSV
                     "
     gene            complement(13429..13911)
                     /locus_tag="PK522_04550"
     CDS             complement(13429..13911)
                     /locus_tag="PK522_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41034.1"
                     /translation="MINKSIIVLALFLVACATTAPKEKPKIFEDQLAGSYSNLARCTI
                     AKLRAEGNWALRSLQFNLKHYKDIEASEVFAYDIRFLPGVFARNSPANPDAVFDYIDP
                     NPSITTYEQRLTNILPGYAFALLIKKNDDTTVNATLEGEMRSGEIAWKHLLECPAKAE
                     "
     gene            14235..15068
                     /locus_tag="PK522_04555"
     CDS             14235..15068
                     /locus_tag="PK522_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNC41035.1"
                     /translation="MEAEHGELIFPTSVKKAIEVKRILDDPARDLDAAARLIRSDPLL
                     SARVVAVANSTAFNRSRKTITNVPAAITLLGLFLVKSLILGVAVRQIAESATTAKREL
                     IAQLWKHSAYVGSIAQVIARRITKHDPETALFAGMIHEICWFYLLFRTKDYPDLFEGD
                     ISSLWNKDDEIDNEGEFIGEIEVGTLLLKALSIPDSIIDTIAFVWRGYQTFPPTSLGS
                     VLLIANQLAPIQSPFTLPIYQTNQDHLVKLDLFFKDYSLSEILAESEEEVDSLTNALC
                     L"
     gene            complement(15076..>15731)
                     /locus_tag="PK522_04560"
     CDS             complement(15076..>15731)
                     /locus_tag="PK522_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNC41036.1"
                     /translation="IITIEDPVEYVHEHINCIITQREVGVDTDSWEAALKNTLRQAPD
                     VILIGEIRDRETMEHAIAFAETGHLCMGTLHANSANQALDRIINFFPEERRAQLLMDL
                     SLNMRALIAQRLIPKKEGKGRVAAIEVMLNSPLIADLIFKGSVHEIKEIMKKSRELGM
                     QTFDAALFDLYEAGLISYEDALRNADSMNELRLQIKLNGSETKNKNVSAGIEHLTIT"
BASE COUNT         4614 a         3278 c         3210 g         4629 t
ORIGIN      
        1 ttttcctggg ggaatttctg gcaatataat ctctcatcct atgacatagt ttatgcatat
       61 ttatcgccta ttccaatgga taaactttgg aaaaaagtcc tacaggaaat gcggccagga
      121 agtattttga taagtaattc gtttatagta cctgatgttc ctcccactga atctattgca
      181 atcaatgatt tcaataattc tgttctttat atctggaaga tatgattcta tttattgacg
      241 tctccatgtc gttccttgtg aactatcctc aagtatgatt ccagcttcag caagcaagct
      301 ccgtatctga tctgcttcct taaaatcctg attttttttt gcctctaggc gctgattaat
      361 cagtaaattt atttcacttt cagaaaattt ttcattttta ttgtttgcta ctaaatcttg
      421 ctgaaggtat ctctgtggct cttgctgcaa taagcccagt atatttgcca gtgacttgag
      481 taaactcgca tcctgcggct tttgagatct atttatttca ctggaaaggt tgaataagat
      541 ggcaattgcc tctggagtat taaaatcatc attcatagct attttaaaac tttgtgcatg
      601 atgattatcc caattaatct tgccagcatt cactgtttca tagcctttta atgctgtata
      661 aaggcgatca agggcatttt tagcactaat cagatgttca agggaataat ttaaagggct
      721 tcgatagtgc gcacgtaaaa taaaaaaacg aatcacttca gcttgaaaat tttttaaaat
      781 ttctcgtact gtaaaaaaat ttcctaaaga tttggacatc ttttcattat ctactcggac
      841 aaatccatta tgcatccagt agtttacata agtctcttca tttgctcctt cactttgagc
      901 aatttcattt tcatgatgtg gaaattgtaa gtcttgacca ccaccgtgaa tatcgaaatg
      961 attacctaag aaatgtccac tcatcgatga gcactcaata tgccagccag gcctcccatt
     1021 tccccacgga gattgccaat aaggttctcc aggtttcact gatttccata aaacaaaatc
     1081 aaggggatca cgcttgtgat gatcaatctc tactctttca cccgcacgta gatcagacaa
     1141 tgatttcccc gaaagctttc catacccagg aaaatgatgt acagaaaaaa atacatctcc
     1201 attgtcagct acgtaagcac aatctttctc gattaactta cggataaggg taatcatctc
     1261 tcccacgtga tgagttgcgc atggctcaaa atttggcttt acggttccaa gggcattggc
     1321 atcttcgtgc atagctttga tgaaacgatt agttaatgtc tctattgact cattcatttc
     1381 ttgcgcacgt ttaattattt tatcgtcaat atccgtaata ttacgcacgt aggtaacgtt
     1441 atacccgatt gattgtaacc aacgaacaac catatcaaat acaaccaata cgcgtccatg
     1501 accaagatga cagaagtcat ataccgtcat accacaaaca taaagtttta cttcacccgg
     1561 tactaatgga ataaactctt cctttgctcg agttaatgag ttataaattt ttagcatcgt
     1621 aactacaatt aaattgtgag ataaataaac tttattgata gaatcaataa ataagataaa
     1681 gttgaatcaa tcctattaat ctaggattct agcagtagca ccaactggtg tatattttct
     1741 cctttcatta ttactgattg aatcattatg ataagactga ttacgctatt actcgcatta
     1801 tcgattaacc tttcagcgtt agctgctcaa ccaattgtta ggattgatac aaatttagga
     1861 ccgatctcgt tagcgcttta ttctgaccaa gcacctaaga cggtagaaaa ttttttgcat
     1921 tatgttcgtg aaggtttcta tgcggagacc atttttcatc gcgttattcc caacttcatg
     1981 atccaaggcg ggggatttga tcaagcatta gttcaaaagc cgacatacca accaatcata
     2041 aatgaagctg cgaatggctt aaagaacgag atcgggacaa ttgcaatggc tagaacgagt
     2101 gatcctcatt cggctacagc tcagtttttt attaatgtag cgaataatac atttcttaat
     2161 tattcctccc cgacagctca aggctatggt tatactgttt ttggcaaggt tatcgaggga
     2221 atggatgttg taaacaaaat tgcagcgctc ccaactggac ccaggggtat ttttgctaaa
     2281 gacgtgccta aggacacggt agtaattaaa gcaattgagc ttctttctga taacaaagaa
     2341 taaaaaatcg attcacatta taggtagatc aaatggttaa atttcacact aattatggtg
     2401 taattacact cgctttatac gatgaacaag caccgcttac agtaaaaaac tttctaagtt
     2461 atgtaagtag tgggcactat gacaatacag tgtttcaccg cgtaattgat aattttatga
     2521 ttcaaggtgg cggatttgat tccaatatga tgcaaaagaa aaccctagag cccattcaga
     2581 atgaagcaag caacggtttg aaaaatgaga cctatacgat tgcgatggct cgaacttcag
     2641 acgttcattc cgcaacctca cagtttttta ttaatgttaa aaataatagc tttctcgatt
     2701 ttacttcacc cacaacccaa ggatatgggt actgtgtttt tggaaaagta attgaaggaa
     2761 tggaagttgt ggatgagata aaaaaagtaa aaacgggtaa ctatgccggg catgagaatg
     2821 taccgctgga aaacgtagtc atagaaagag ccgagattgt ctagttcgaa tatttaatta
     2881 tccatgggta tgcgcgaacc atatgatata gccggttaat actaagcagg cgagaagcga
     2941 taagtaagct gtaagccgat acttttgcct caacgaagct tgcgaataaa cagaaataat
     3001 aaacggataa cgcttgtcct taggtttagt aataatattg tgctcaggct ctagtaatcg
     3061 ttgctggtat atttgttgtg attctgcact cgctatttga cgtgcagatt cccattcttg
     3121 ctggtcgatt tggccatctt tattcgcatc aaaacgtttt agtaaatgct gagaatcttt
     3181 tttccaatcc agtattagat tattcataat ttcacggata ctatgctgtg aagcagccga
     3241 gcttgtacta aattgaccta acacataaag cgattgtcct ggcaaaatca gtgattcact
     3301 ataacgatat cgattgttat tgctatgcaa gactgattga ctttcaagta tctgtgtttg
     3361 attgggccat tcggtgttgc cataccatac tagcttctcc tcaccctgga tttcagcact
     3421 atcaggatcg acaaagcagc tagttgaccc atctgtcaat ttaaatgaat gatggctaat
     3481 attgctatag atcacattcc agttgattcg tgattgtcct ttctcaataa gaatttcctg
     3541 acactcaatc tgactccggt accataagca gcgatgattt gaaagtggcg cataaatagg
     3601 tttatcctct atcaacgctc ctttcccttc tatttcaaca taaccctgat gggcagaacg
     3661 taaatttgcg gttggagtat cttcaataaa ccgaagtcgc ttccaatttt tgaagaaaga
     3721 atagaagctt atcagagcga tcagaatgac cgccattaaa ataaattcat attcctcatg
     3781 cgtcaaagaa gatgaaaaag tcaccacagc gagtaagcga tttgctagct aaaaagttta
     3841 cctacatcaa catcttgttt ttcagtagca ttgaattgaa gtaaatctcg cgcattaaac
     3901 ttaaaccaat tcgcaataat aacctctgga aattgctcaa tacgaacgtt attgatttta
     3961 acggcttcat tgtagtattc gcggcgatcc gcgatgctat tttctaggcc tgtaattcgc
     4021 gattggagat gttgaaattt ctcactggat ttgagttctg gataatcttc gacaacagca
     4081 aacaagttac caagacctgt tcttaacata ttttccgcag cacctaatgc tggtaagtta
     4141 ccggcatcac gcgcgctagc gacctgagat cgtgcagcaa tgacttgctt aagggtttct
     4201 tgctcaaaac ccatatattg cttacaggtc tcaacaagct tgggcaactc atcatggcgt
     4261 tgctttagaa gaatatcgat attagaccac gcctgcgata cgctatgctt gacatcaacc
     4321 agactgttat aaatcatgat cccgtaaata accgcaatta ccagcattgc aatcaaaaga
     4381 tagattatcg tcgtagtatc caatgttaaa cctccgctat gttaggtttt gtagaatacc
     4441 tgagtcatgc attgttagat aagaaaaaaa gaaacttgtc cccgccctat ttcttcgtaa
     4501 aaattaaatc ccacacctgg tggcctaatt taattccacg ttgttcaaac ttagttaagg
     4561 ggcgacttgt cgggcgttga gcataatctt tattattatt gattaaactc gtttgattac
     4621 ttaaaaccat aagaatctgc tctgcgtaat cttgccagtc agttgcaata tgtaaatagc
     4681 cccctgattt aatgcgttca caaagcaatg ttacaaacgt tgcctgaata agtcgtcgtt
     4741 tatgatgtct ttttttgggc catggatcag ggaaaaaaat atgaacgcca tcaagggatt
     4801 ctttctcaat catcaggttt aatacagcta ccgcatcata ttgaatgatg cgcaaattac
     4861 ttaaaccctg gagatcaatt aattttaata agctgccgat acccggtgta tgaacttcga
     4921 tagccaaata atcattatca ggtgattgtt gagcaatcgt ggccgttgtt tcgcccatac
     4981 ctgagcctat ttcaagtatc ttgggtgcac agcgcccaaa aatggtgttt aggttaagtg
     5041 cttcttgctg gaaagggatg ctgaaatccg aaaataatgt ttcatatgct ttgcgctgcc
     5101 catttgtgta tcgcccatta cgaatcgcat agctacggat agatatagca gatgacattt
     5161 taaacctgaa taatcaacac caagtagatt acttatgcga tgacagcggt gaaataccat
     5221 cgataggaga gcttgggctt gctgagtaaa attttttctc catccgaccg gcaagataag
     5281 cttctctccc cgcttcaacc gccttgcgca tcgccgaagc catcatgacg ggatcttttg
     5341 ccgctgctat cgcggtattc attaacacgc catcacagcc aagttccatg gcaatagtag
     5401 catcagaagc tgttcctact ccagcatcta ccaaaactgg cacagttgcg cgatcgatca
     5461 tgatttgtaa gttccatgga tttaaaatac ccatacccga tccaattaat gaagcaagcg
     5521 gcataatcgc aacacagcca acttcttcaa gctgttttgc aacaatcgga tcatctgacg
     5581 tataaaccat gacttgaaaa ttttctttta caagcgtctg cgcagctttg atggtctcta
     5641 caatattggg aaacagtgtt ttagaatctc ccagtacttc gagtttcacc aaggaatgtc
     5701 catccagtaa ttctcgtgct aaccgcaacg ttcgaatcgc ttcttcagca gtatagcaac
     5761 cggctgtatt gggcaataac gtaaattttg atgggggaag aacatccagt agatttggct
     5821 catctggatt ctgtccaata ttagtacggc ggattgcaac cgtaatgatc tctgaaccac
     5881 ttgcttctat ggctgctcgc gtttcagaaa aatctttata tttgcccgta ccaactagca
     5941 gccgggattt atagcattta cctgcaatta taaaatcatc cataaataac taagcttttg
     6001 aagtcatgat ttgcagcaaa tgagcaagtc cagtaataag attacacaac taaccaccac
     6061 caactgccac cacaatctcg agtcgatcac cctctgtcac gatatagtcc gaaaatctac
     6121 ttcttggaat aatttcacca ttatgctcga ttgcaatgcg cttattctga agccccatat
     6181 tctcaagcaa ttgttgcaaa ttgagcgagg attcaaattg ttgcggctca ccgttgataa
     6241 tcagttgtat catttgaaat ataacaagtg tagggtaaac tttgtggttt tctaattaaa
     6301 tgattttagc acgcgggtgt agctcaatgg tagagcagaa gcttcccaag cttaagacga
     6361 gggttcgatt cccttcaccc gctccatagc agcgaccgat tggaaaagtt gggtgcagta
     6421 aatacgttat aatgtaacta cttaaaatct tggatctttt catgaaacgt aagatcaggg
     6481 ggctatttgt ttttttctgg atagtaaccc tattaataat aagtcatgct gtattagctg
     6541 ataataaaat ttctcaaccc gaatctaaac ttatcgtcgg ttgggtagaa tttattcgcc
     6601 tcgaaccttg gggtatgaag atgcgtgccc gtattgacac tggcgccaat acatcttcga
     6661 tgagcgcacg tgatattcat caattcaaga aaggaaacga ggactgggtc aaatttgtac
     6721 tggattttaa aacggacaaa ggtaaaccca atcgctcagt tgagatcgaa cgacctatca
     6781 cccgcatcgt aaaaatcaaa caacatcaag gaacactaca agaacgccct gtcgtcaata
     6841 tggatatttg tatcgccaac caagtccata atattgagtt taacttgatt gatcggcgtg
     6901 ggctgaatta tccggttctg ctgggacgcc gtgccctaga aaatttagca ttagttgacc
     6961 ctgctcggac ttttattgaa aaagctgatt gtggtcacat caagaaaaaa aagaaaaaag
     7021 cagatacacc accagattca atcagtcttg aatgagacaa ctacatttat tgttcttaac
     7081 gttactgcta tgcgctattg gattagggct ggcagcctat aaagtcgata cactgggttt
     7141 accactgaca gcttctgcac aaaccgaggt ttggaatgtc gaagcacgca tcagcttcaa
     7201 agccaaaccc gatagtggag ttaaagtcag cttaccgctc ccttttaatc cttcaggcta
     7261 cacagtcctc aatgaagatt ttgtgggcgc taattatgga ctagcaaccg agcaagatga
     7321 aggtgggcga gttgcattat gggcaaaaag gcgtgctcaa ggtaaacagt tattatttta
     7381 ccgcgcagtt atatttgaaa atgaagagaa aatagctgat tccagtaaaa ttccaagtta
     7441 tcctgctgtt ccagattatc ccgaacattt cttacccgtc attcagggtt tattagataa
     7501 atcccgtcag caatctgcgg ataccctttc gtttgcacaa caagtcattc agcaactcaa
     7561 tgccacagca cctgcgccgg aattaaagtt actcattgag cacattaata cgtccaaaaa
     7621 tctagcgaaa gggattagct ggattctagc cggtgcaaga gttccagctc gggttatcca
     7681 agggttacgt ttacaagatg gtaagcgtta tagcgaactc gaagcttggg tgcaagttca
     7741 tgatggtaag caatggcgaa cacttaatac caaagatggg caagaaggcc tacctaccaa
     7801 ttttgttatc tggcagatcg gtgatcagaa aacgttttcg gttgaaggcg gaactgaaat
     7861 tagtttgcaa ttttctgttg caaaagcact ccgtgagctc gttgatgtcg ccgagtcccg
     7921 cgctatcttg aaaggctctc gcatcatgga gttctcactg tattctttac cagtgcaaac
     7981 ccagaatgtt tataaagtct tgttaatgat tccattaggc gcatttgtcg tggttttgat
     8041 gcgtaatctc attggagtta aaacattcgg tacattcatg cccgttctga tcgcacttgc
     8101 gtttagggaa actgaactcg tctggggact tgccttgttt tgtttgatcg tgggtctagg
     8161 gttattattg cgcgcttatg ttgagcaact caagttattg ctcatccctc ggcttgcttc
     8221 ggtattgacc atggtggtac tgctcatggc agggaccagc gtagtaatgc ataagcttgg
     8281 actagaaatg ggcctatcag tagcactttt ccctatggta atcatgacca tgaccattga
     8341 acgcatgtcg ctgacttggg aagaagcagg accaatcgaa gcgtttaagc agggcgccgg
     8401 cagtttgttg gttgcgacaa ttggttattt ggtgatgaat atcgatgaat tgcagcattt
     8461 attatttgtt ttcccagaac tattactcgt cgtgttggcg gtaactatct tatttgggcg
     8521 ctactccggt tatcggttaa cggaactgtg gcgattccgc gctttagcaa aacggtagct
     8581 aaatgcaatg ttttcgacct ggctaaatct caagcgtgct ggggtaatcg gtatgaatcg
     8641 gcgtaatgcc gattttatcc tacgctacaa cgaacgttca ctttatcctt tagtcgatga
     8701 caaattacgc accaagcaac tagcgattgc tgctggaatt gcagttcctc agctatacgg
     8761 agttatcgaa atcgagcacg atatcgttaa ccttcctggc attgttaagg attacaagga
     8821 ttttgtcatt aaacctgccc atggttccgg tggtaatggt atcgctgtca tcattggtcg
     8881 catcaatgag cgatttcaat acaataatgg gagtctcttg actgaagaag agctcgaata
     8941 tcacatttcc aatatcctaa gcggtatgta tagtctggga ggacagcctg atcaagcatt
     9001 aatcgaatat cgcgtgaaat tcgacccctt atttgaatcg gtcagttacc gcggtgttcc
     9061 tgacattcgc accattgttt tcaggggtgt gccggttgct tcaatggtac gtttaccgac
     9121 acggttgtcc gatgggaaag ctaatttaca ccaaggcgct gtgggcgcag gcattgacct
     9181 tacctcgggt gttactactc gcgcagtttg gcaaaatcgg ccgatcgata tccacccgga
     9241 tactggatca gctatcagcg ggctcgccat tccccactgg aataaattac taacactcgc
     9301 cgcacagtgt tatgagttag tcggattagg atacctcggc gtcgacgtgg tattggatca
     9361 tgaactgggt ccattggtat tggagctcaa tgcacgcccg ggcttgtcca ttcagattgc
     9421 caatggaaaa ggaatggaga agcgattgga atatattgat cggcttagtg aaattccaga
     9481 gaaagcacaa gatcgtgctg cacttgctca gtcactgttt aaagcagaaa actaaagcca
     9541 gggcagcaat cactgaatag caggattaat tacaataaac gcctagccag gccatcctat
     9601 ggactctcga tttattattt acggcgatat cggcggcaca aaaaccattc tgcggttggc
     9661 aaaacagcaa aatagtgatt tattgcacat acgggaacag cgctatgcga gccaggatta
     9721 tcaaaatcta gatgaaatca tcaacgattt tttaatcgga ttaccagata cgaatgcagc
     9781 aaacaaggcc atctcagccg cctgttttgc aattgcaggc ccgattgctg cgcaacaagc
     9841 acgattaact aatctaccat ggctaattag cgcagcagat ctttcaagaa aatttgctat
     9901 tccccaagtt aagttgatca atgattttga agcaacagca ctgggggttg agaaattaac
     9961 acagcaagat ctcgaagtta tccaaatagg aaatatgaat gaagctagta tgcgcgttgt
    10021 gctcggtgcg ggcactggta tgggagttgc ctggatagca agtattggaa accgctatat
    10081 tccctttcca actgaggcag ggcataccga ttttgcgcca ataaacgatt tgcaaatcga
    10141 attactcaac ttcttagctg caaaatttgg ccatgtttcc atcgagcgag ttctctctgg
    10201 acccgggtta accaatattt ttaatttttt tcaacaacgc gcgaaccatc tgcggtcctc
    10261 ctccttgcca gaattgagtg aagatgaggg tcaattgatt attcaattgg cgctagagca
    10321 aaaccatgtc acagcaatcg agtccctaca gctattcact gaaatctatg gtgcatttgc
    10381 tggaaattta gctttaacag gactatgtcg tggtggggtt tacattgctg ggggcattgc
    10441 accacgaatt ctgcattacc taaagacgga acgctttatc caagcattcc gcaacaaagg
    10501 tcgtttcaca ggactaatgc gtgaaatacc gctctctgtc atcctaaata cccaggttgg
    10561 gctccttggt gcggaaagtg aagcgcaacg attacaaaat ttgctggtag caaattcgag
    10621 taataatact aatacttagc gttatttata ggcagataaa ctggcacgga tgaattgatt
    10681 caaaggatga acccatgcat cttgttcaac aaagaagcta tcattgtgct ttccttgcaa
    10741 cacgatgaat tgtttgggat taggtgcttt atcaaaaagt gcttgggcat gcttgaaggg
    10801 cacaatctca tcctctgggc tgtgagcaac gatgacggga caggtaacag attgcaaata
    10861 tttgctggta ttatagtgaa aacgtgccat gagtcgcaca ggtaataaag gatataaatc
    10921 ggctgctata tctggaatag aggtaaaggt tgaagccaac actaatgcgc ccggatcatt
    10981 tttcgatgca agccatgcag caatggctcc tcccagtgac tctccaaaca agacgatgtg
    11041 cgaacccgga atcgtcttat tctcgatcag gtaattccat gcggtaagtg cgtcttgata
    11101 agtaccttct tcagttgggg taccgctact gttaccgtac ccccgataat caacaatgaa
    11161 tgtgttatag ccaagttggt gaaatattga aaggtagccc atgcgatgag aaatattgcc
    11221 tgcattccca tgcaaaaaaa ggatggttgc agttgtttct ttggctgcag gtacaaacca
    11281 cccatgtagt ttttcttgat cgctagtcgt cagtacaact gattcataag gcatcgagaa
    11341 ttggtcggga gtattgacga tttcacggtc aatttctgga tggtaaatga gatgcgattg
    11401 agcgaagaaa atgaatatcg cccaagctgc ataaatggag atgccgagta tgatcaaccg
    11461 aagtgttgtg cgcatacgga taacaaagaa agaacttttc ttatcaatcc aagtaaatta
    11521 gaaacttgct attataatcc tgctttaact ctctttagga tttgctatga acttagatcg
    11581 tgtagactca ggtcgggatc ttcccaatga cttcaatgta attatcgaaa ttccgatgaa
    11641 ttccgagcca atcaaatatg agatggataa aaagacgggc gccatgtttg tcgatcgctt
    11701 catgtcaacc gcgatgcgct atccatgcaa ttatggttat attccgcaca ctttgtcgga
    11761 agataaggac cctgttgatg ttctagtcat ttctcctacc ccgctcatta ctggtgtcgt
    11821 tgtgcgttgc aggccgattg gtttattgaa catgaccgat gaagccggtg aagatgtcaa
    11881 actaatcgca gtgcccattg ataaattatg tgggctttat atacatgtgc aaacgctgga
    11941 tgatttaccc gaattcatgc gcgcacaaat tgcccatttt ttcgctcatt acaaagattt
    12001 agagcggggc aagtgggtta aaatcgaagg ctggggagat gttgaagcag cccgagccga
    12061 gatcatgaca ggggtaaagc gctacaattc cgctaagaat aaacctaaat tttgattgct
    12121 agtgttaata atttacctgg gtaaatcgta tgacagctag actgattggc ccgcagcaaa
    12181 gcccgatgac caggcccatt gaaagttgaa tccacctaaa tgcccggtga catcgacaac
    12241 ttcgccaata aaataaagcc ctggtctttt ttttgcttcc agagtccgag aagaaagttc
    12301 attagtatcg actccaccga ttgtgacctc tgcttttcgg taaccttcag taccagtagg
    12361 ccaaatcaac caagcctgaa taacttcagc aatttttgca agctgccggt cggacaaaat
    12421 accaatggcc ccagtaaaaa catattggtc acataaattc tgggcgagac gtttaggtaa
    12481 tcgttcagcg agtacacctc caatatgtag ttttggttgg ttattctttt tggctttgag
    12541 cactgaataa agatcgatat cgggaataag attgactcgt atcgcttttc cctgttccca
    12601 ataagaagag atttgcaaaa tcgatggccc acttaatcct cgatgggtaa acaataaccc
    12661 ttccgaaaag gactggcggt tgaattgtac cgttgcatca atcgaaacac cgcttaaaga
    12721 tttgcattgc tccaataagg tgccatcaaa tgttaatggt acgagcccag ggcgtggttt
    12781 aatcacattg atatcaaatt tctttgcaac ttcataccca aaattagacg cgccgatttt
    12841 ggggatagat aatccccccg ttgcgatcac caaggaacga caggtatgga taccccggtt
    12901 ggtatccagt cgataaccat cagcgagcgc ctcaatttgc gtgacacgtg tttgcaacgc
    12961 aaacgatact tttgcctgat gcgattccga tagcagcata tcaattatct gctgcgatga
    13021 atgatcgcaa aaaagctggc ccagtttctt ttcgtggaaa gcaatgtggt gcttatttac
    13081 aagattaatg aaatcatgag gcgtaaattg agcgagtgcc gatttgcaaa aatgaggatt
    13141 ttgagaaagg taatgctgtg gctgggtgaa aatattggtg aaattgcatc gtcctccacc
    13201 agaaattcgt atcttctctg caaacttagc catatgatcg ataagccata cacttcgacc
    13261 tcgcttacct gcttcaatgg cacacatcaa accggctgca cctgcaccga tgataattac
    13321 gtcatatgaa ttcattgatc cagttgatcg attatgcgtt cgagtacact cgatcagctt
    13381 taattacatt aaaaaataaa ggtaaattac ttagcgagat agcaaaagct attctgcttt
    13441 tgctggacat tcaaggaggt gtttccaagc aatttcaccc gagcgcatct caccttctaa
    13501 tgttgcattg actgtcgtgt catcattttt cttgatgagt aaagcaaacg catagcccgg
    13561 taagatattc gtcagccttt gttcatacgt cgtaatacta ggattagggt cgatataatc
    13621 aaagaccgca tcaggattag cgggtgaatt acgggcaaaa acaccgggta agaaacgaat
    13681 atcgtaagca aagacctcag aagcctcaat atccttgtag tgtttcaaat taaactgtag
    13741 cgaacgaagc gcccagtttc cttcagctct taattttgcg atagtgcagc gagctaaatt
    13801 cgaataactc ccggccaact gatcttcaaa gatttttggt ttttcttttg gcgcagtcgt
    13861 tgcacatgcc accaagaaca aagctaaaac aataattgat ttattgatca cgattattac
    13921 tccaaaaaaa atagacagag cgagaatgaa ttactttatt gatatcagaa gaaaatttga
    13981 agactgaata gtaaaattct aacttacttt aagtgttatt ctcatggcct tactcattct
    14041 aatgagctcc cttagttgag attaaaagtt cccccaatat ctgattagca aaatccatgc
    14101 taacttactg attaaaagta atttactaaa ttaataaatt gttaaaccga taccctaaaa
    14161 ggtggtctaa ttgttaacta ttaattctaa ctgcatttac catggataaa gctgagatct
    14221 taaatagaat taaaatggaa gcagagcacg gggaattaat ttttccgact tcagtaaaaa
    14281 aagcaataga agttaaaaga attctggatg acccagcccg tgaccttgat gctgctgccc
    14341 gacttattag atctgatcca ttattgtcag caagggtagt cgctgtcgca aattccacag
    14401 catttaatcg atcaagaaaa acaatcacca atgtacccgc tgcaataaca ttgctgggac
    14461 tatttcttgt aaagtctctg attttagggg ttgcagtgcg ccaaattgca gagtctgcaa
    14521 caaccgccaa acgcgagtta attgctcagt tatggaaaca ctctgcctat gttggttcaa
    14581 ttgctcaggt cattgcacga agaattacaa aacatgatcc tgaaaccgct ctgtttgctg
    14641 ggatgattca tgagatatgc tggttctact tactattccg aacaaaagat taccccgact
    14701 tattcgaggg tgatatttcc agtttatgga ataaagatga tgagatcgat aatgaaggtg
    14761 aatttattgg tgaaattgaa gtaggtacat tgctattaaa agcgttgagt atccctgatt
    14821 ccatcattga taccattgct tttgtttggc gcggctatca aacctttcct cccacttcac
    14881 tgggtagtgt cctcctaatc gctaaccagc tggcacccat tcaatcccct tttacactcc
    14941 ccatttatca gacaaatcaa gatcatttag tcaaacttga tttattcttt aaagactatt
    15001 cgcttagtga aatactagcg gaatctgaag aagaagtaga ttcattaacg aacgccttat
    15061 gcttataagc ttgcatcagg taatcgttaa atgctcaata ccggcgctga catttttatt
    15121 tttggtttct gacccattga gtttgatctg taagcgcagt tcgttcatag aatctgcatt
    15181 tctaagcgca tcttcataac taatcaagcc cgcttcatat agatcgaata gtgctgcatc
    15241 aaaagtttgc atacctagct ctctcgattt tttcatgatc tctttgattt catggacgct
    15301 tcctttgaaa attagatcag caattaaggg tgaattgagc ataacttcaa ttgctgctac
    15361 acgcccttta ccttctttct taggaatcaa tcgctgcgca ataagtgctc gcatattgag
    15421 ggataaatcc atgagtagtt gagcacggcg ctcttctggg aaaaagttaa taattcgatc
    15481 cagggcttgg ttagcactgt ttgcatgcaa tgtccccata catagatggc ctgtttcagc
    15541 aaatgcaatg gcgtgttcca ttgtttcgcg gtcacgaatc tccccgatta agataacatc
    15601 aggggcttga cgcagcgtat tttttaacgc ggcttcccag gaatcggtat ctacgccaac
    15661 ttcacgctgg gtaataatgc aattgatatg ttcatgaaca tattcaactg ggtcttcaat
    15721 ggtgatgata t
//
LOCUS       DAOQLJ010000041        15478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1380_length_15478_cov_6.686313, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000041 DAOQLJ010000000
VERSION     DAOQLJ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 15478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15478
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1380_length_15478_cov_6.686313"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PK522_04565"
     CDS             complement(<1..527)
                     /locus_tag="PK522_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNC41037.1"
                     /translation="MFNLLNNNFLGNYFSTDMAVDLGTANTLIHVRGRGIVLNEPSVV
                     AIRQEGGSSGRKVIQEVGLAAKQMLGRTPGNITAIRPMKDGVIADYTVTEQMLKQFIR
                     KVNPPRLFSANPRIVISVPCGSTQVERRAIREAAIGAGARKVELIEEPMAAAIGAELP
                     VETPTGSMVVDIGGGT"
     gene            674..961
                     /gene="gatC"
                     /locus_tag="PK522_04570"
     CDS             674..961
                     /gene="gatC"
                     /locus_tag="PK522_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNC41038.1"
                     /translation="MEILLTDVYRIADLARVEIQTDEAQAALEQLSNIFNLIEQMQAV
                     DTAEIEPMSHAQDMKLRLRQDIVSEADRHELFQSIAPQVEAGLYLVPKVIE"
     gene            1011..2462
                     /gene="gatA"
                     /locus_tag="PK522_04575"
     CDS             1011..2462
                     /gene="gatA"
                     /locus_tag="PK522_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041352745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNC41039.1"
                     /translation="MLGQSLKYLSTQLNDKRISSVELTTEYLKQIRSLNTEFNAFITI
                     DEAQSLEQARQADQLIATGNASPLTGIPIAQKDIFCTKGWLTTCGSKMLANFTAPYDA
                     TVIERFNQVGAVNIGKTNMDEFAMGSSNETSFFGPVKNPWDIHAVPGGSSGGSACAVA
                     AGMAPAATGTDTGGSIRQPAALCGISGIKPTYGLVSRFGMIAFASSLDQGGPMARSAE
                     DLAMMLNVMVGFDERDSTSLQREVEDYCRDLERPLSGLRIGLPKEYFEQGLSDEVAYA
                     VKQAIDEYQKLGANIIEVSLPNAKLSIPVYYVIAPAEASSNLSRFDGVRYGYRAEQYI
                     NLDDMYKKTRAQGFGQEVKRRILIGTYVLSHGYYDAYYIKAQKLRRLIANDFTDVFKQ
                     CDVLMGPTTPTVAFDLGEKCDDPIQMYLSDIYTSSVNLAGLPGMSIPIGFGKKSRPIG
                     LQIIGNYFKEAQMLNVAHQYQKVTDWHLQTPIK"
     gene            2503..3942
                     /gene="gatB"
                     /locus_tag="PK522_04580"
     CDS             2503..3942
                     /gene="gatB"
                     /locus_tag="PK522_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNC41040.1"
                     /translation="MQWETVIGLEIHAQLSTKTKIFSGASTVYGAKPNTQACGIDIAL
                     PGVLPVLNVGAVERAIKFGLAVGATINSPSIFARKNYFYPDLPKGYQISQYEIPIVQG
                     GSVTIQIGDIVKVVRLTRAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLLEIVSEPDMR
                     SSAEAVAYAKALHTLVQWTGICDGNMQEGSFRCDANVSVRPFGTDKFGTRCEIKNLNS
                     FRFLEKAIEYEVRRQIEILEDGGRIIQETRLYDADEDETRTMRSKEDAHDYRYFPDPD
                     LLPLVISPELIAQFKQQLPELPEAKRNRYQNDYGLSDYDAAVLTTSKAISDYFETMVQ
                     SLPDQPKLCANWIMGELIGKLNKENTEISNCSIKPEQLTKLLQRIIDGTISGKAAKTV
                     FESMWQREHAGDVDTIIAATGLIQVSDQGEIEKLIDTVLEANPQQVNDYQNGKEKAFN
                     SLVGQVMKASKGKANPMQVNTMLRDKLKR"
     gene            4175..4672
                     /locus_tag="PK522_04585"
     CDS             4175..4672
                     /locus_tag="PK522_04585"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNC41041.1"
                     /translation="MIHDKKVNVLFVCMGNICRSPTADAVFKQYIQTAGLANHVYVDS
                     AGTHAYHTDEPPDKRAQNAARKRGYSMHELRARLVGSQDFIKFHYILAMDKNNLEILK
                     ERCPPTHHHKLDLLMKYSTQWNTLQEIDDPYYGGSYGFERVLDLVEESSQGLLTHLLN
                     HDLKN"
     gene            complement(4755..5195)
                     /gene="flgN"
                     /locus_tag="PK522_04590"
     CDS             complement(4755..5195)
                     /gene="flgN"
                     /locus_tag="PK522_04590"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HNC41042.1"
                     /translation="MHLSAEDKRKLLDILMAERETVLAIIDKLKKEQESLVNGKVDDI
                     FKYAAEKDFQLYHLNNLYKQQCELLQSSDSQYIILDKSNSYNSNFNQLWTDILNLISS
                     AKQLNNTNETIVSMNLQYSQNLSFALHGNYQNHALYNARGMKTQ"
     gene            complement(5195..5485)
                     /gene="flgM"
                     /locus_tag="PK522_04595"
     CDS             complement(5195..5485)
                     /gene="flgM"
                     /locus_tag="PK522_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNC41043.1"
                     /translation="MKINKVNQLSTNNTVNKGNQAEKINSNKSEELEDPQSNVHLSST
                     LSSLGDTTAANISKIAEIKQAMAEGRFKVNPEVVADRLLETVKELIQSNKKA"
     gene            complement(5694..7127)
                     /locus_tag="PK522_04600"
     CDS             complement(5694..7127)
                     /locus_tag="PK522_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC41044.1"
                     /translation="MTTANFSWNRPTQELDSTLEKKITYQKLSDGILISNIRLFIIFR
                     WAIVLGLILFQIAAIVASDTLTQLGIKDQEDWPLAVTVILTIANIAYAYALDYCQPSK
                     YNTPSINIWVQIIIDLLCLSVVVHFVGSTETPAPFFYILHIALACIFFSTLESLFVTA
                     IVSAMYSILLVIESGMFFQVPQSMLIDAHYSGEKLHKGHILVWMAALNTLFLTVWYVV
                     SRLAIILKQHEQHLREAYEQIHQAQIAKDQYAMLMTHQLKAPLDAIRSKINLINGGYC
                     GPVPTEIADVLDRIDKRGSSMAKLILDVLRLERLKTAGRNLEAIEYISISSIIDKCVE
                     KLKPVADARQVELILSIEDFRAHGIADQIEILIENIASNAISYSHVGKSVEISSIVNK
                     NQNTAVVSITDHGIGIEEKDLPNIFNEYFYSPRAVLHNQSSSGIGLSLVKIAAANNNL
                     QLKVTSEPEVGTTFFITFTDIQQIDIH"
     gene            complement(7162..7542)
                     /locus_tag="PK522_04605"
     CDS             complement(7162..7542)
                     /locus_tag="PK522_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC41045.1"
                     /translation="MTKKIAHIDDDPDIRDTVKRVLEMHGYQVDSYHTSSDFISSLDN
                     SEKVPDLAILDVMVESMDAGLTTYIQLHERFPKMHAIFMTSLGDTILPYFENKSQEWV
                     CILEKPVQPTILLTIIQDRLGKAS"
     gene            7943..9733
                     /locus_tag="PK522_04610"
     CDS             7943..9733
                     /locus_tag="PK522_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNC41046.1"
                     /translation="MTSELIDPIIVGLTILGLFFAFIKEWVKPDIAVVVAVSVLMILG
                     LLDSKQVLSVFSNSAPITVATLFIMSAALEKTGCVAKLSGWIASIAGENEKKLLAMIM
                     LTALAVSPFINNTPVVMVLMPAVIAVAVKIQSSPSRFLIPLSYATILGGMMTLIGTST
                     NILVDGVARDMGLAPFGMFEITAPAIVMGLIGYLFIYLLAPRLLPIRETLAQQFMGAS
                     DRAFMTELFIPENSRLAGKTLVEAKLNQVGVTILKIFRADEAITSPSDNFRLEVGDRL
                     VVHSKSSAMMDLHSSDWIGLQNNASNHDLETLRRKDAVIIEAIVGSSSRYVQRPIRDL
                     DLLARYGIHLIAIHRKDASIGDIVDDFQLQFGDVLLVEGTPSQIKRFCENGDLFALTE
                     GKVTSTRQHKAPIALATMGGVILLAAFKILPIESLAIIGAAIVLVTGCIRSDEAYKAI
                     EWPLIVMIFGMLAISIAMRDSGLADMAARSLLSIGQGLDPWWMLLAVIFLTSVLTEIL
                     SNNAVAVLMTPIVIGMADHMGVDARPFVVGVMFAASMSFATPIGYQTNTLVYSAGNYK
                     FSDFARLGIPLNILLGAGAALLIPLFWPFH"
     gene            9801..11186
                     /gene="nhaC"
                     /locus_tag="PK522_04615"
     CDS             9801..11186
                     /gene="nhaC"
                     /locus_tag="PK522_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HNC41047.1"
                     /translation="MTKSLREPSLLQALICIVGIFLTIALGLLLVGISIHILVFLCII
                     WVSIHAAYLNYSYTDIQRMMSDSIHHALPAIYIFILMGMVIASFMYSGTIASLIYYGL
                     SLLTPSIFLPVGLILCCLMSIVTGTSWGTVGTIGVVLIGIADAIHIPIFLAAGMIVSG
                     ATFGDKLSPLSDTTNLAAMTAGVGLYQHIYAMVYTTLPSFILALGLFSVIGLQFAESA
                     IPIEQISEIQIALASLFHLDLWITLLPLICMLVLSLLRFSPAICMTCSIVIAVIISIL
                     YQDKLIVEVLNALWLNTPANTGIENIDALLGRGGIYNMAWTLILSIMALALGGVLYQA
                     KFLTVLINSFINKIRRFSTLVATTITAGFVSNLGMGEAYISIILNCQLFKALYTKLDL
                     HHAILSRSVEEGTTVTAPLIPWTTAGAFYAATLSVAVLDYAPYAFFNLLNPIVSVLMA
                     IFGIGLLRGQR"
     gene            11291..11686
                     /locus_tag="PK522_04620"
     CDS             11291..11686
                     /locus_tag="PK522_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41048.1"
                     /translation="MDTTNRVFISFALKDTELRDKLIEQLKKTHPALNVVFMPEKKSW
                     ESAWKAECQGQVDGCDGAIGIITKNTSRADGQLWELRCALDSQMKLLLVGDDEICEMS
                     AKKLPELIRDEDILPWDSSSISSFLNRLK"
     gene            complement(11703..12863)
                     /locus_tag="PK522_04625"
     CDS             complement(11703..12863)
                     /locus_tag="PK522_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HNC41049.1"
                     /translation="MSVNFDEIIQRAGTNCTKFDKRLAVFGDSHVTPLWVADMDFAAP
                     HEVTAALISRAKHPIYGYTFAPDSLYEALISWVKQRYHWSIKQEWIVLCPGVVPSLTA
                     AILALSEVNESTIILPPVYFPFFSAVTKSNRQLTLCPLSYHNQRYEIDFDRLAALEDN
                     SRILLFCSPHNPVGRVWPQSDLEQLIKIAQKKNWIIISDEIHADLTYPEYQHTPLARL
                     AEEFENIITALAPSKTFNIPGLNLSVLIVPNAKFRQAILNFFDQLHINPANPFSITAF
                     ETAYQKGANWLDELISYLSCTRDQVADYLTKYLPAIQLVKPEGTYLLWLDCRSMRMTD
                     SELKRFFIHQAGVGLSPGILFGDEGSGFMRMNIGTPRSNVLAALEKIKKAYV"
     gene            complement(12860..14971)
                     /gene="ovoA"
                     /locus_tag="PK522_04630"
     CDS             complement(12860..14971)
                     /gene="ovoA"
                     /locus_tag="PK522_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HNC41050.1"
                     /translation="MNTTLFQRTPILDGGDPEAKREEIRRYFHTTLDRYELLFETLVS
                     DEAYYSKPITLRHPLIFYLGHTATFFTNKLILARAITHRINPRLESMFAVGVDEMSWD
                     DLDTTHYDWPMVDEVRHYRSVMRETVDRLITELPITLPIGWDSPWWTIIMGIEHERIH
                     LETSSVLIRQHALKFVKPHPHWQPCKKTGIAPENQLVTVPAGLVQIGKTIADATYGWD
                     NEYGSHHAEIPSFQASRYLVSNKEFLSFVEANGYDTDQYWEPEGLGWLRHTRAQHPTF
                     WVKQDSHWYLRLMTEVTPMPWDWPVEVNYHEAKAFCNWKTAHDKLPVRLPTEDEWYRL
                     YDSSNLSDVPATQLASGNIHLDYYASSCPVNEFKHGDFFDIVGNVWQWTETPTYPFTG
                     FEVHPHYDDFTTPTFDDRHNLIKGGSWISCGNESLKSSRYAFRRHFFQHAGFRYVVTE
                     TPALMQGSHYETDKLMSEYAEFHYGDSYFNVPNFPATLASIAITAMGNRPAHKALDLG
                     CASGRSTFELARHFEYVTGVDFSARFINQGVQLIQQGLLRYTLTDEGDLVFYKERSLA
                     ALGLESVKSKVEFYQGDACNLKPILTGYDLILAVNLIDRLYDPAKFLVNVHERINLGG
                     LLLIASPYTWLEEHTKRESWIGGFKRDGESFTTLDGLKSILGNHFKLIQGPQEVPFVI
                     RETRRKFQHTLSEVTIWEKIS"
BASE COUNT         4576 a         2785 c         3230 g         4887 t
ORIGIN      
        1 gttccaccac cgatgtcgac gaccatcgat ccagtagggg tctctacagg taattctgct
       61 ccaatagctg ctgccattgg ttcctctatg agttccacct ttctggctcc agcaccaatg
      121 gcagcttctc gaattgctct tctttcaact tgagtcgaac cacaaggaac tgaaataacg
      181 atacggggat ttgcagaaaa aagtctgggt ggatttactt tacgaataaa ttgcttgagc
      241 atttgttcag ttactgtgta atcagcaatt acaccatctt tcatgggtcg aatggcagta
      301 atatttccag gtgttcttcc taacatctgt tttgcagcca aaccaacttc ttggatgact
      361 tttctaccac ttgagccacc ttcttgccga attgcaacga cagagggctc gttcaaaaca
      421 ataccgcgcc cccggacatg aattaaggta ttggctgtac ccaaatcgac tgccatatca
      481 gttgaaaagt agttccctaa aaagttattg ttcaggaggt taaacatatt ggcgaatttc
      541 cattaaagat gacagatttt gataaataaa tgcttttgca gcgatgttaa ggtgtgacgt
      601 gtaaaatata tgcgttcatc agatatgtca tgatactcta ttacccattt taagataaag
      661 cgccgaatta cagatggaaa ttttactaac tgatgtctat aggattgcgg atctagctcg
      721 agttgaaatt caaactgatg aagctcaagc agcgttagag cagttatcca atattttcaa
      781 tttaattgaa cagatgcaag cggttgatac tgcagagatt gaaccgatga gtcatgccca
      841 agatatgaaa ttgcgtttac gtcaagatat tgtttctgaa gccgatcggc acgaactttt
      901 ccaatcgatt gcaccgcaag tagaagcggg actttattta gttccaaagg tcatcgaata
      961 attcgtgcgg aatacttcct cgctattttt ttccttattt tagaagtgtt atgttaggtc
     1021 aaagtcttaa gtatttatct actcagttaa acgataaaag aatttcgagt gttgaattaa
     1081 caacagaata ccttaagcaa atccggtcac tgaatactga attcaatgcg tttattacga
     1141 tcgatgaagc gcagagtctt gagcaggcgc gacaggcgga tcaattaatt gcgacaggaa
     1201 atgcttcacc gctgacgggg attccaattg ctcagaaaga tattttttgt actaaaggtt
     1261 ggttaactac gtgtgggtca aagatgttag ctaattttac agcgccctat gatgctacgg
     1321 tgattgagcg ctttaatcag gttggtgctg taaatattgg caagactaac atggatgagt
     1381 ttgcaatggg ttcgagtaat gaaacctctt tctttggacc agttaagaat ccatgggata
     1441 ttcatgccgt tccaggtggt agttctggtg gttctgcttg tgctgttgcg gccggaatgg
     1501 cgcctgctgc aacaggaacg gataccggtg gatcgattag gcagcctgct gcattatgtg
     1561 gtatctcagg catcaaacca acctatggac ttgtttctcg tttcggtatg attgcttttg
     1621 cttcaagcct tgatcaaggt ggacctatgg ctagatcagc cgaagacttg gcaatgatgc
     1681 taaatgtcat ggtcgggttt gatgagcgcg actccacaag tttgcagcga gaggttgaag
     1741 attactgccg tgatttagaa agacccttat ctgggctgcg tattggttta cctaaagaat
     1801 attttgaaca aggcctaagt gatgaggtag cgtatgcggt taaacaagcg attgatgaat
     1861 atcagaaact gggcgctaac atcattgaag tttcattacc aaacgcaaaa ctttcaattc
     1921 ctgtttatta tgtgattgcg ccggctgagg catccagtaa tttgtcgcga tttgatgggg
     1981 tacgatatgg atatcgtgct gaacaatata taaacctgga tgatatgtat aaaaaaaccc
     2041 gggcacaggg atttggccaa gaggttaaac gtcgaattct gattggtact tatgtgcttt
     2101 cacatggtta ttatgatgct tattatatta aggctcagaa gttgcggcgc ttgattgcga
     2161 atgattttac agatgttttt aagcagtgtg atgtactgat gggtccgaca acacctaccg
     2221 ttgcatttga tctgggagaa aaatgtgatg atcccattca aatgtattta tcggacatat
     2281 atacaagttc tgtgaatttg gcagggttac ctggtatgtc aatacctatc ggatttggga
     2341 agaaaagtag accaattgga ctacaaatta ttggaaacta ttttaaagag gcacaaatgt
     2401 taaatgttgc tcatcagtac cagaaggtaa ctgattggca tttacaaact ccaataaaat
     2461 aaacttgagt aatcgactat ttgttagaaa ggctatttgt tcatgcaatg ggaaacagtt
     2521 atcggtcttg aaatacacgc acaactttcc acaaagacta agattttttc tggtgcctca
     2581 actgtttatg gggcgaaacc caatacccaa gcatgtggga ttgatatcgc gttaccaggg
     2641 gttttgccag ttcttaatgt cggagcggtg gaacgtgcga ttaagtttgg gctagcagta
     2701 ggagcaacaa ttaattcacc ttcgattttt gcaagaaaaa attatttcta tccggaccta
     2761 ccgaaaggct atcagattag ccaatatgaa ataccgattg tacaaggggg cagcgtaacc
     2821 attcaaattg gggacatagt aaaagttgta cgactgactc gtgctcacct agaagaggat
     2881 gctggaaaat cactccacga ggattttcat ggaatgagcg gaattgattt aaatcgagcg
     2941 ggtacccctc tgcttgagat tgtttcagaa cctgatatgc gtagtagtgc tgaagcagta
     3001 gcctatgcta aagcattaca tacacttgtt cagtggacag ggatttgcga tggaaatatg
     3061 caagaaggtt catttcgatg tgatgctaat gtgtcggtta gaccctttgg tacagataaa
     3121 tttgggacac gttgtgaaat taaaaactta aattcctttc gctttcttga gaaagcgatt
     3181 gaatatgaag taagaaggca aattgagatc ctagaagatg gtggtaggat aatacaagaa
     3241 acgcgacttt atgatgccga tgaagatgaa actcgaacta tgcgcagtaa agaagatgca
     3301 catgattatc gctatttccc agatcctgat ttgttaccgt tagtgatatc acccgaattg
     3361 atagcacaat ttaaacaaca actcccagaa ttacctgaag ctaagcgaaa tcgatatcag
     3421 aatgattatg gactttcaga ttacgatgca gcggtattaa cgacaagtaa agcaatctca
     3481 gattattttg aaactatggt ccaatcttta ccggatcaac ctaagctttg tgccaattgg
     3541 atcatggggg aactaattgg caagcttaac aaggaaaata ccgagatatc aaattgttct
     3601 attaagccag agcaattgac taaattgctt cagagaatta ttgatggaac tatttcgggc
     3661 aaagcagcta aaaccgtttt tgaaagcatg tggcaaagag aacatgctgg agatgtggat
     3721 acaataattg ctgctacggg tttgatacag gtttcagatc aaggagagat agagaaacta
     3781 attgatactg ttttagaagc taacccacag caagttaacg attatcagaa tggtaaagaa
     3841 aaagctttca attcattggt agggcaggtt atgaaagctt caaagggtaa agcaaatcct
     3901 atgcaagtta ataccatgct gagagacaaa ttaaaaagat agtttaattg gaaatgtaag
     3961 ttaacgattc ctaagcggag taaatacgat ttaggctctt ttgcaggatg gtgattttag
     4021 cgagaataca caacgaatta gatcttattt gcgaataatt gatgaaagtt agcttaattt
     4081 tttctagaat attaactggg ataaattaat aaattcgctc atattaataa gtgatttcta
     4141 atctaataat aagaatagtt taagtgttaa ctaaatgatt cacgataaaa aagtcaatgt
     4201 tctttttgtc tgcatgggga atatctgtcg gtcaccaaca gcagatgctg tattcaaaca
     4261 atacattcag acggctggtc ttgcaaatca tgtgtatgtg gattccgcag gaacacatgc
     4321 ttatcatact gatgaacctc cagataaaag ggctcaaaat gcagcaagaa aaaggggcta
     4381 tagcatgcac gaattaagag cacggttagt tggaagtcaa gattttatta agtttcacta
     4441 tattctggca atggataaaa ataatcttga aatccttaaa gaacgctgcc ctccaacgca
     4501 tcaccataag ctcgatttat taatgaaata tagtactcaa tggaatactc ttcaggaaat
     4561 tgatgatcca tattatggag gaagttatgg ctttgagcga gttcttgatt tagtggagga
     4621 atcgagtcag ggtttattaa cccatttact aaatcatgat ctcaagaatt aatcatatag
     4681 atagtaaata ttattttact tgattgttta gtgacgaatg aattgatttt aaacttgtta
     4741 agttattaag ctacctactg tgttttcata cctctagcgt tatataacgc atgattttgg
     4801 tagtttccat gaagggcaaa agaaagattt tgtgaatact gtagattcat agaaacaatt
     4861 gtttcatttg tattattgag ttgttttgca gatgaaatta ggttaaggat atctgtccat
     4921 agctgattga agttagaatt ataactatta cttttatcca gtatgatata ttgagaatca
     4981 gaagattgaa gaagctcaca ttgctgttta tagagattat ttaaatgata gagttgaaaa
     5041 tctttttcag cggcatattt aaatatatcg tcaactttcc cattaactag ggattcttgc
     5101 tcttttttta gtttatcaat aattgcaaga acagtctcac gctctgccat taagatatct
     5161 aataactttc tcttatcttc agcagataaa tgcattatgc ttttttattg ctttgaataa
     5221 gctcttttac tgtttctagt agacgatcag caactacctc ggggttaact ttaaaacgtc
     5281 cttctgccat tgcttgttta atttcagcaa ttttgctgat attcgccgcc gtagtatcac
     5341 ccaagcttga aagagtggag cttaaatgaa cattggattg cggatcctcc agttcttcag
     5401 atttgtttga attgatcttc tcggcctgat ttcctttgtt tacggtatta ttggtagaca
     5461 actgatttac tttatttatt ttcaagataa tcccctcgaa tgctagaatt tgaacaatct
     5521 tacgtcatta atgtaataat cttgaatctt tctattagtt gattaataaa ttcgataacg
     5581 aacatttact gtgtggtcta attgcatatt aaattctttg tcatacatga gaaattaaga
     5641 aaagaaggta aaagatagca tatttctgaa ttaattgatc atttgcactt gaatcagtga
     5701 atatcaattt gctgtatgtc agtgaaggtt ataaaaaaag tagtacctac ttctggttcg
     5761 ctggtaacct tgagttgtag attattatta gcagctgcaa tctttacaag cgataaaccg
     5821 atgccacttg atgattgatt atggagtact gctcttgggg aataaaaata ttcattaaaa
     5881 atattaggta aatctttttc ttcaatccct attccatgat cagttataga tacgactgct
     5941 gtattttggt ttttattaac gattgaacta atttctacag attttcctac atgagaatag
     6001 gaaattgcgt ttgaagcaat attttcaatt aaaatttcga tttgatctgc aattccatga
     6061 gcgcggaaat cctctattga taatatcaac tctacttgtc ttgcatctgc aactggttta
     6121 agtttttcca cacatttatc aataattgat gaaatactta tgtactcgat tgcttcaagg
     6181 tttcttcccg cagttttcaa cctttctaaa cgaagcacat ctaaaataag tttggccatg
     6241 ctcgagccac gtttatcaat acgatctaat acatctgcaa tttctgttgg tactggacca
     6301 caataacccc cattgattaa attgattttg ctacgaatcg catctaatgg tgcttttagt
     6361 tggtgagtca ttaacatggc gtattgatct ttagcaattt gtgcttgatg aatttgttca
     6421 taggcttcac gcagatgctg ctcatgctgc tttagaatga tggctaaacg tgaaacaaca
     6481 taccaaactg tcaggaaaag tgtattaagt gctgccatcc aaactaggat atggccttta
     6541 tgtagttttt ctcctgaata gtgggcatca attagcatag attgaggtac ttggaaaaac
     6601 attccacttt ctatgactaa taaaatcgaa tacattgcag aaacaatcgc tgtcacaaat
     6661 aagctttcta acgttgagaa aaatatacaa gctaaagcaa tgtggagaat gtaaaagaac
     6721 ggagcaggcg tttcagtact accaacaaaa tggacaacaa cggataagca gagtaagtca
     6781 ataattatct gtacccagat atttatactg ggtgtgttat attttgaggg ttggcagtaa
     6841 tcaagcgcat atgcgtaggc gatgtttgca attgtcaaaa tgactgttac agccagtggc
     6901 caatcttcct gatcttttat acctaattgt gtaagcgtgt cggaagcaac tatggcagca
     6961 atttggaata aaattaaccc aagaacaata gcccatctaa aaataataaa taatcggata
     7021 ttgctaatta ggattccatc acttaatttt tgataggtta tttttttctc aagtgtagaa
     7081 tctaattctt gagtgggtcg attccaagaa aaattagcgg ttgtcataat taatgagtcc
     7141 gattaggtaa atgtgttgta actagctagc tttaccaagc ctatcttgaa tgatggtaag
     7201 taatatcgtt ggctgcaccg gtttctcaag aatacatacc cattcttgtg atttattttc
     7261 aaaataaggc agaatcgtat cccctagaga ggtcataaaa atcgcgtgca ttttaggaaa
     7321 gcgctcatgt aattggatat aagttgttaa accggcatcc attgattcga ccattacatc
     7381 gagtatagcc aaatcgggaa ctttctcaga attgtcaagt gagctaataa agtcactgga
     7441 tgtatgatag ctatctactt gatagccatg catttctaga acacgcttta ccgtatcccg
     7501 aatatcggga tcatcgtcaa tatgggcaat tttctttgtc atataatatt cctttattaa
     7561 attctgatag aagtattaat aggttttgat tgttatttca ataaattaaa tagtgaaatt
     7621 gcccatggac cttcagccta ataattaatg tgtaaccaaa gggcagctcg atctatggtt
     7681 tcactgaaat tgaacatcac aatatctatt gaataatagc taggtaactc attcctattg
     7741 gaatcgatgc ttcaaaatac tataccctgc tttttcctaa gagcaagtga tttcatgaaa
     7801 atccgctagt aatcttagct gacgcaaatt acaatccagt gttagcgtga tattttgatc
     7861 actataattc agtgatagtt aagtttttgt aactttatat atgcattttt gtaattttct
     7921 gcaaagaaaa atggggtgat ttttgacttc agagcttatt gatccaatta ttgttggctt
     7981 gacgatatta ggtttgtttt ttgcgtttat caaagaatgg gttaaacctg acattgccgt
     8041 tgttgttgcc gtttcagttt tgatgatttt ggggctgttg gatagcaagc aagtgttaag
     8101 tgtttttagt aacagcgctc ccattacagt cgcgacgttg tttattatga gtgctgcgct
     8161 cgagaaaaca ggttgtgtcg ctaagttatc tgggtggatc gctagtattg caggagaaaa
     8221 tgaaaaaaaa ttattagcaa tgatcatgct gacagcgcta gctgtatcac cattcattaa
     8281 taataccccg gttgtcatgg ttttgatgcc cgcagtcatt gcggtagcgg ttaaaattca
     8341 atcatcacct tctcgattct taatcccttt atcttacgca acaatattgg gtggaatgat
     8401 gaccttgatc ggtacatcaa ccaatattct agtggatggc gttgcgcgtg atatgggtct
     8461 agcgcctttc ggtatgtttg aaatcactgc gcctgcgatt gtaatggggt tgattggtta
     8521 tctttttatc tatttacttg cgccaaggtt actaccaata cgagaaacac tcgcacaaca
     8581 attcatgggt gcaagtgatc gtgcttttat gactgaacta tttattccag aaaattcacg
     8641 gttagctggt aaaacattgg tggaagctaa gttaaaccaa gtaggggtaa cgattcttaa
     8701 gatttttaga gcggatgagg caattacttc tccttctgat aatttccggc tcgaagtggg
     8761 agatcgatta gttgtgcata gcaaaagtag tgccatgatg gatttgcatt cttcggattg
     8821 gattgggtta caaaataatg ctagtaatca tgatcttgaa acactgagac gtaaagatgc
     8881 tgtcattatc gaagcaatag ttggttctag ttcacggtat gtacaaaggc caattcggga
     8941 cttggatttg cttgcacgct atggaatcca tttgattgcc attcatcgta aagacgcgag
     9001 cattggtgat attgtcgatg attttcaatt acaatttggt gatgttttat tggttgaagg
     9061 aacaccatca caaattaagc gtttttgtga aaatggtgat ttattcgcat taacggaagg
     9121 gaaagtaacc agtacacgtc aacataaagc accaattgcc ttagcgacaa tggggggcgt
     9181 gattttactc gctgcattca aaatattacc aattgaaagt ttagcgatta tcggtgctgc
     9241 aattgttcta gtcacgggtt gtatccgttc tgatgaagct tataaagcaa ttgagtggcc
     9301 actcatcgta atgatatttg gtatgctcgc aattagtatt gcaatgcgcg attctggatt
     9361 ggccgatatg gctgcgcgca gcttgcttag tataggtcaa ggacttgatc cttggtggat
     9421 gttgctcgct gttatctttc tcacttcggt tcttacggaa atccttagca acaatgccgt
     9481 tgctgttttg atgacaccaa tcgttatcgg aatggcagat cacatggggg ttgatgcgag
     9541 accctttgtc gtcggtgtaa tgtttgctgc aagtatgagc tttgcaaccc ccattggtta
     9601 tcaaacaaat acattggttt atagtgcagg gaactataag tttagtgatt ttgcacgcct
     9661 aggaattccg ctcaacattt tgctcggtgc tggagccgca ttgctgatac cacttttctg
     9721 gccctttcat tagccgtcta tcccgcttct aaacgttctg catgatattg cggattgatg
     9781 gatctaaatc gtaaacgact ttgactaaat cacttcgtga accttctttg ttacaagctt
     9841 taatttgcat tgttggaatt tttctcacca ttgcgttggg gttacttttg gtggggattt
     9901 caattcatat tcttgttttt ctgtgcataa tctgggtaag tattcacgct gcctatctca
     9961 attacagcta taccgatatc caacgtatga tgagtgatag tattcatcat gcacttcccg
    10021 ctatttatat ttttatttta atgggaatgg tgattgctag ctttatgtac agcggtacta
    10081 ttgcgagtct tatttactat ggcctatcgt tattgacccc ctcaattttt ctgcccgtag
    10141 gtttgatact atgctgttta atgtcaattg taacaggaac atcatgggga actgttggga
    10201 cgattggtgt tgttttgata ggaattgctg atgcgatcca tataccgatt tttcttgctg
    10261 ctggaatgat cgtatcaggg gctacatttg gtgataagtt atcaccgtta tctgatacga
    10321 caaatcttgc tgcgatgaca gcaggggtcg ggctttatca gcatatttat gcgatggtgt
    10381 atacaacgtt acctagcttt atcttagcgc tcggtttatt cagtgtaatc ggattacaat
    10441 ttgctgaatc tgcgatacct atcgaacaaa tttcagaaat tcaaatcgct ttagcaagtt
    10501 tatttcacct tgatttatgg atcaccctat taccactcat ttgcatgctc gttctcagtc
    10561 ttcttcgctt ttcacctgca atttgtatga cttgcagtat tgtgatcgca gttatcattt
    10621 cgattctgta tcaggataaa ctaatcgttg aagtactcaa tgctctttgg ctaaatacgc
    10681 ctgctaatac gggaatagaa aacatagacg ccttattagg ccgaggtggt atttataata
    10741 tggcctggac attgatatta tcgatcatgg cactggcgtt aggtggtgtt ctttaccaag
    10801 cgaaattttt aacggtatta atcaatagct ttattaacaa gattaggcgg tttagtacct
    10861 tagttgctac gacaatcaca gctggttttg ttagtaattt agggatgggt gaggcttata
    10921 tttcgattat tctaaattgc caattattta aagcgctcta cacgaaatta gatttacatc
    10981 atgccattct ctcgcgttct gttgaagagg gtactaccgt gacagcacct ttaattccat
    11041 ggacaaccgc aggtgctttc tacgctgcga cacttagtgt tgcagtactg gattatgctc
    11101 cttatgcatt tttcaattta cttaacccta tcgtttctgt cttgatggca atttttggaa
    11161 taggattact caggggacag cgttgacgct taataacctg gatgctatat tgattttagc
    11221 taccaaagag agcaatgaga ggtattgttg agcaatacac ctgaacaata ataagtcaaa
    11281 gaggaaacct atggatacga caaatcgcgt ttttattagc tttgcactta aagatacaga
    11341 actcagagat aaactgattg agcaattgaa gaaaacacac ccagcgttga atgttgtttt
    11401 tatgcctgaa aagaaatcgt gggaaagtgc atggaaggca gaatgtcaag ggcaagtgga
    11461 tggctgtgac ggtgcaattg gaattattac caagaataca agccgtgctg atgggcaatt
    11521 atgggagctc cgttgtgcgc tggattcaca aatgaaattg ttactcgttg gtgatgatga
    11581 gatttgtgaa atgtcagcta agaaactacc agaactcata agggatgagg atattttacc
    11641 ttgggacagt tctagtattt catcattctt aaatcgacta aaataaaacg agagagcggt
    11701 agttagacat aggctttttt aattttctca agcgccgcca gaacattaga tcgcggtgtg
    11761 ccaatgttca tacgcataaa accgctccct tcatcgccaa ataaaatacc tggactaaga
    11821 ccaacaccag cctgatgaat aaaaaagcgt ttgagttcag aatctgtcat tctcatcgag
    11881 cgacaatcaa gccataacag ataagttcct tctggtttaa ccagttgaat cgctggtaag
    11941 tatttggtta gataatcagc cacttgatcg cgcgtgcaac tcaagtaact aatcagttca
    12001 tctaaccaat tagccccttt ttgataagca gtttcaaatg cggtgatact aaacggattg
    12061 gccggattga tatgtaattg atcaaaaaaa ttgagaattg cctgacgaaa ttttgcattg
    12121 gggacgatca atacagaaag gttaagccct ggaatattaa aagttttgct tggcgctagg
    12181 gcggtaatga tattttcgaa ttcttctgcc aggcgtgcga gaggagtatg ctgatattct
    12241 ggataggtga gatcagcatg gatttcgtca gagatgatga tccagttttt tttctgagca
    12301 attttaatta attgttctaa atcagattga ggccatactc gacctacagg attgtgggga
    12361 gagcagaata gcaatatccg tgagttatct tctaatgctg ctagccgatc aaagtcaatc
    12421 tcatagcgct gattgtgata gctcagaggg cagagggtta gttgtcggtt tgatttggta
    12481 actgctgaaa aaaatgggaa ataaaccggt gggagaatga tagttgactc gttcacttct
    12541 gaaagtgcca agatcgcagc agtaagcgat ggaactactc cagggcagag gacgatccat
    12601 tcttgcttaa ttgaccaatg ataacgttgt tttacccagc tgattaaagc ctcgtataaa
    12661 ctatctggtg caaacgtata accatagatg gggtgtttgg ctctgctgat aagcgccgca
    12721 gtcacttcgt gaggtgcagc aaaatccata tcagctaccc acaaaggagt aacgtgacta
    12781 tctccaaata cggctaagcg cttatcaaat ttggtacagt ttgttcctgc cctttggata
    12841 atttcatcga aattaacact caagaaattt tttcccaaat agtaacttca gataatgtat
    12901 gttgaaattt tcgtcttgtc tcgcgaatca cgaacggaac ttcttgtggc ccttgaatca
    12961 gtttaaaatg gtttccaaga atggatttca atccatcgag tgtcgtaaaa ctttctccat
    13021 cgcgcttaaa gccaccaatc caggattctc gtttggtatg ttcttctaac caagtataag
    13081 gagaggcaat cagcaataac cctcctaaat tgatgcgctc atgaacattg acaaggaatt
    13141 ttgcgggatc ataaagtcga tcaattaaat tgacagcaag aatcaagtca tatcctgtca
    13201 gtatgggttt gagattgcaa gcgtctccct gataaaattc aactttgctc tttacgcttt
    13261 caagccctaa agcagcgagt gatctttctt tgtaaaaaac aagatctcct tcatcagtca
    13321 atgtatagcg taatagacct tgttgaatta actgcacccc ttgattgata aaccgggctg
    13381 aaaaatcaac ccctgttaca tattcgaaat gccgtgccag ctcaaatgtt gaacgtcctg
    13441 aagcacaacc taaatcgagt gctttgtgag ccggtcgatt tcccatcgca gtaatcgcta
    13501 tgctggctaa tgtagctggg aaattgggta cattaaaata actatctccg taatgaaatt
    13561 cagcatactc tgacatcaac ttatccgttt catagtgaga gccttgcatt agcgctggtg
    13621 tttcagtgac aacataacga aatccggcat gctgaaaaaa atgacgacgg aacgcataac
    13681 gcgatgattt taacgattcg ttaccacatg aaatccatga gccgcctttg ataagattgt
    13741 gccgatcatc aaacgtaggt gtcgtaaaat catcataatg aggatgaacc tcaaatcctg
    13801 tgaatggata ggtgggggtt tccgtccatt gccaaacgtt gccaacgata tcgaagaaat
    13861 cgccatgctt gaattcgttg accgggcaac ttgaagcata ataatcgaga tgaatattac
    13921 ctgacgctaa ttgagtagca ggtacatctg acaagttcga gctatcgtat agccgatacc
    13981 attcatcttc agtaggtaag cggaccggaa gtttatcgtg cgctgttttc caattacaaa
    14041 atgctttagc ttcatgataa ttcacttcga ctggccaatc ccatggcatt ggggtaactt
    14101 ccgtcataag acggagatac caatggctat cttgttttac ccagaaagtt ggatgttgtg
    14161 ctcgcgtatg tcgaagccag ccaagacctt ctggctccca atactgatct gtatcgtaac
    14221 cgttggcttc aacgaaactc aaaaattctt tattactcac caaataacga ctggcctgga
    14281 aagagggaat ttcggcgtga tgtgaaccat actcattatc ccaaccatag gttgcatcag
    14341 cgattgtttt gccaatttgt accaatccag ccggtacggt taccaattga ttttctggag
    14401 caattcctgt tttcttgcag ggttgccagt gcgggtgagg tttaacaaat tttaaagcat
    14461 gctgacggat cagtaccgaa gatgtttcaa ggtggatgcg ctcgtgttcg atacccatta
    14521 tgatggtcca ccaggggcta tcccacccaa tgggtaatgt tatcggtagc tctgttataa
    14581 ggcgatcaac ggtttctcgc attacgctgc ggtagtgacg aacttcatcg accattggcc
    14641 aatcgtaatg ggtcgtatct aaatcatccc agctcatttc atcgacaccc acggcaaaca
    14701 tagactctag cctggggtta attcgatgtg taatagctcg cgctagaatt aacttattcg
    14761 tgaaaaaagt agccgtatgc cctaaataaa atataagcgg atggcgcaat gtaataggct
    14821 tactataata ggcttcatcg cttactaaag tttcaaaaag taactcatag cgatcgagag
    14881 tcgtatgaaa gtaacgacgt atctcttcgc gtttcgcttc ggggtcgccg ccatccagaa
    14941 taggcgttct ctgaaaaagg gtggtattca tagaaataat caacttaact ttcgcttttc
    15001 ttaatttgct attatacctt ctaagaaatt ttgcagagaa ttaacttggc ggaatactca
    15061 gtagggattt ttagaacgga tacttgacta agaaaggggg tatttgattt tctatatgat
    15121 ttttaagcgg gcgcgagatc tgattcagct aaatccataa ttaataatat agtatactta
    15181 taatcagtgg ggttaatcct agatttactt aagttatttt tcatctttca tttcttatca
    15241 ctgttattta cccaattcat tttttcttag tttaaaagaa gttttaaagt ctttttcatt
    15301 catgaagtta tcattctttt ccaatctttt tgattatctg ccgatttaaa tgcatttttc
    15361 caaagcgctc aatagaagaa atattgaaac acaatcgatt aaaagaaata tttttttgat
    15421 tggaatgatg ggagctggaa aaactacgat tggtaaatca cttgcaaact atctggat
//
LOCUS       DAOQLJ010000042        15356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1401_length_15356_cov_6.739363, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000042 DAOQLJ010000000
VERSION     DAOQLJ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 15356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15356
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1401_length_15356_cov_6.739363"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1737)
                     /locus_tag="PK522_04635"
     CDS             complement(<1..1737)
                     /locus_tag="PK522_04635"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNC41051.1"
                     /translation="METDISSPTKKRFLTRKHLAISLGVIFSIVVLFGVLGYFWLPGF
                     AKAKLEQVLSETLARPVTVQSIDIKPFTLEVAVRGFRIGEKSTVSDQTTFFSFDQLYV
                     DLSAESLIHLAPVVTTISLVAPKIYVTREDKNQFNFSDLIEKFGQTPEEKPQEPKKPT
                     LFSINNISIQNGEITFADRIKNSQQHITAINLSIPFIANFKNVLTNWVEPNLSAKINE
                     APVTLSGKVLPFSDKQEATLSLKLDDIDLTNIDEYSPIPLGIRLLSGKFDSDLLVSFT
                     HVIDEPPNIDLSGQIALKGIQIENRTVEMPYVLGVKQFNLKLNEVSFNETKPVKVGTT
                     FESIAITPIGEKQALLSLPKIELDSITVDRARHKIDLGTILLDGINGLIKRQPDGQLE
                     LNRMLAVTASSNSMQSEEDKVKKLSKPIQRNVSAIIPIPDKKPDNRVTSLAQTQAREQ
                     ELVAPSVITIPLPEHKPQIVARPEDTKQPQANMGKMAEETSSQSAPAAEETDQPWAIY
                     IDQIKLSDGAITFEDYTVSKVVPMHINPLNLTVRNIDLSGAQPVELSLQAQVNQQGSV
                     ETKGTLAWAPLAA"
     gene            complement(1817..2278)
                     /locus_tag="PK522_04640"
     CDS             complement(1817..2278)
                     /locus_tag="PK522_04640"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNC41052.1"
                     /translation="MLRIKLLGCFIAIVSMLFSFSAFAYKEHSAEPHSAASGVSALQK
                     IDTTVGNGEEAIVGKTVKVHYSGWLYDESAPDKKGKKFDSSYDRKEPFAFMLGAGRVI
                     KGWDQGVIGMKVGGQRTLIIPSSMAYGAQGAGNVIPPHATLIFDVELVGFE"
     gene            complement(2391..2963)
                     /locus_tag="PK522_04645"
     CDS             complement(2391..2963)
                     /locus_tag="PK522_04645"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC41053.1"
                     /translation="MPFSFSNQLSAIIGYAEQQKPLSILDIGVGMGQYGFLLRTNLEN
                     INLFEINGANARLRDKNQWRIKIDGIEGYAGYLTPVHNYAYNRILIGDALELLPTLAS
                     NSYDLVLAIDILEHFYKEQGVHFLKECRRIAKGSSLISTPKEFMEQDVPANPFENHRS
                     VWNDEDLKRCGFDTFLPNPFSWIALASHHE"
     gene            3130..4032
                     /locus_tag="PK522_04650"
     CDS             3130..4032
                     /locus_tag="PK522_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNC41054.1"
                     /translation="MSQQSNSSSISTDERIISGSNGWVMLVSLLALFAFSLFFTMTPG
                     PPIKLFGGILLAMLALFGCKGLFTLEPNQAAVMVFFGNYVGTVNESGFFWVNPFYNKT
                     RVSLRINNWNTPVLKVNDERGSPIEIAAVIAWRVQDTARAIFDVESTLNYLQIQSESA
                     VRQVASSHAYDDTEMIDGKRKKSLRTDLDAIAGLLRESIQEHVNVAGIVIEEAKIAHL
                     AYAPEIANAMLRRQQAEAVVMARQKLVDGAVGMVEVALKNLEEKQIVSLTSDQRAVLV
                     TNMMTVLLSESGAQPVIQMTQTTP"
     gene            4079..4861
                     /locus_tag="PK522_04655"
     CDS             4079..4861
                     /locus_tag="PK522_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HNC41055.1"
                     /translation="MTHALKLWPLLTGTIRYEKTISTHNRGHGQWIDAPILAYLIETT
                     QGRILYDVGCDYHKLSDPALRQQFFASMYPVIETPQMQAEQRIPHYLERLGLTTADID
                     LILIGHLHFDHVGGLCDLPGCEVHLHQAELAAARTKQDSGVFPDELLQSDRWRLQHEE
                     YELAPGVQVLTTPGHTAGHLSLMIELPQGQPIILCGDAADLNENLTDEIAPGCCWQDN
                     FELALASIRKLKSIAISEQAQLWPNHDMVFFKQLPVFPTGHE"
     gene            5014..5235
                     /locus_tag="PK522_04660"
     CDS             5014..5235
                     /locus_tag="PK522_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41056.1"
                     /translation="MKYIIIAAALAGLSLAGCGGKPSQALPTTEKEAYEKIISGQSLD
                     CQFGIDANGNCLKEGEDPRPYGGKGKPGH"
     gene            5572..8442
                     /locus_tag="PK522_04665"
     CDS             5572..8442
                     /locus_tag="PK522_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNC41057.1"
                     /translation="MQLTANSTSTVLNPIPHGEFSEGSSNQGVQLSLYKIIRRNGAVV
                     AFEPSKISVAMTKAFIAVEGGQGAASARVREVVARLTEEVVNALTRRQSGGGTFHIED
                     IQDQVELALMRSGEHDVARSYVLYREERARERAKQQEKQTTAKTPLIHIVENGQRIPL
                     DTKQLTELVDAACADLGNSVDSSLILKSTLKDLYDGVPANEVRKSLILSARALIEKDP
                     DYSFVTARLLLHSIRLEALGENISQKEMESRYADYFPNFIKQGIDAGLLDERLAQFDL
                     TQLAAELVASRDMQFGYLGLQTLYDRYFLHINDKRIELPQAFFMRVAMGLALNEIDRE
                     ARAIEFYHVLSKFDFMSSTPTLFNSGTCRPQLSSCYLTTVPDSLDSIYEAIKENALLS
                     KFAGGLGNDWTPVRAMGSRIKGTNGKSQGVVPFLKVVSDTAVAVNQGGKRKGAVCAYL
                     ECWHLDIEEFLELRKNTGDDRRRTHDMNTAVWIPDLFMKRVMENADWTLFSPSDTPDL
                     HEKYGKAFETAYLSYEEKASRGELSLYKQVSAQQLWRKMLTMLFETGHPWITFKDPCN
                     IRSPQSHVGVVHSSNLCTEITLNTSDKEIAVCNLGSVNLLAHIKDSKLDTEKLKRTIS
                     TAMRMLDNVIDINFYAVTKARNANLKHRPVGLGIMGFHDCLYQLGISYASQEAIEFAD
                     RSMEAVAFHAYWASTELAEERGCYATYRNSLWDRGILPQDTLDLLRDERGGFVDVDTT
                     TTLPWDKLRARIKKHGMRNSNCLAIAPTATISNIIGVSASIEPTYQNLYVKSNLSGEF
                     TIANRWLVNDLKKHELWDEVMIADLKYYDGAIGKIDRIPPLIRSQYATAFEVDPYWLI
                     ESAARRQKWIDQAQSLNIYIAGVSGKKLDEIYKLAWLRGLKTTYYLRSLGATTAEKST
                     VYTGALNAVPTSAGATMANAQQTAATEIKYCAIDDPGCESCQ"
     gene            8485..9672
                     /locus_tag="PK522_04670"
     CDS             8485..9672
                     /locus_tag="PK522_04670"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNC41058.1"
                     /translation="MLTFDDEPLQSEAIQGKSILSNSRPDWAESTGITPVAPPPSTIS
                     LPQNEQLAEDIALRRVSVEDKRIINCKTDVNQLVPFKYKWAWEKYLSACANHWMPQEI
                     SMSRDIALWKDPNGLTEDERRLVKRNLGFFVTADSLAANNIVLGTYRHITNPECRQYL
                     LRQAFEEAIHTHAYQYIVESLGLDEGEIFNAYHEIQSIRDKDEFLIPFIDTLTDPKFK
                     TGTLETDQQLLKSLIVFACIMEGLFFYVGFTQILALGRQNKMMGAAEQYQYILRDESM
                     HCNFGIDVINQIKLENPHLWTKEFREEITTLMHKAVELEYRYAEDTMPRGVLGLNAAM
                     FKEYLRFIANRRCQQIGLDALFPEASNPFPWMSEMIDLKKEKNFFETRVIEYQTGGVL
                     SWD"
     gene            9921..11312
                     /locus_tag="PK522_04675"
     CDS             9921..11312
                     /locus_tag="PK522_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNC41059.1"
                     /translation="MLKIKKIQILMLSIAISSVLLLLPYQVEAASTAQTKKNTYAAKG
                     RIASANRNNNVGDLWTRVRSGFSMSELQGHEVRQNENGYTRHQKFIDHMVEQSRRYLF
                     HIVEEVERRKMPTEIALLPIIESAFNPVAYSHRHASGLWQFVPATGRAYGLEQNWWHD
                     DRRDVIAATNAALDYLQKLYRMFGDWKLTVAAYNWGEGALKRSIDENHGGDLKVSFHD
                     LQLPTETKNHVAKLIAIRNIIANPSQYGVKLKPLPNRPYFKQVKITQHMDVKLAAELA
                     DLPEHEFNALNPAYHRPVIRIKDKPRTLLLPVGSVKTFEVNLENYDKSLVSWRVYRVK
                     ENETLDDLSIKYRVDIAELAQVNGLSEYDTIEEGQTLLVPRKRNRNSNDDRLFVAQPT
                     FSELHMQPVKVSPIIHIVQKGDTLYDIAKRYSVDVRELKSWNGNKERLAIGQKLIVRL
                     ADSNRNQSVTKPS"
     gene            complement(11441..12010)
                     /locus_tag="PK522_04680"
     CDS             complement(11441..12010)
                     /locus_tag="PK522_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41060.1"
                     /translation="MLNLKGSSILVGCALLLSFSLQAENKSDNAGKHLALNLVGTGTM
                     YENMVPDIDGDGQDDLAMCFDVQVFDAHTNRALGKGTDCLAMVTPTGTGVALVGTTYF
                     HLPGGTLITRGKTSVQPVFHPTVTPRGQVITHITGASSDGNAIVGGTGRFAHAKGTAR
                     LSGMVSMANFSGDVGDPISFDCLFVVALD"
     gene            complement(12327..13421)
                     /locus_tag="PK522_04685"
     CDS             complement(12327..13421)
                     /locus_tag="PK522_04685"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNC41061.1"
                     /translation="MGQELYVGVMSGTSLDGIDVVLVDFRLSPPKLVKTYFLPYCASL
                     KKDLLALHLQGENELRRSALLANVLSSSYAQAINNLLIAAEISSESIIAMGCHGQTIR
                     HCPDSGYSIQLFNPALLAELTGITVVADFRSRDIAAGGQGAPLVPAFHQACFGHQTSH
                     RIIVNIGGIANITSLSPEDIVIGFDCGPGNMLMDAWCFENQGQSYDDNGNWASKGESH
                     SLLLEALLSYPFFSLPPPKSTGRDCFNLDWLRMHLNGTELPVDVQATLLELTVQSIVR
                     SIQSYFPAATEIYLCGGGAHNARLVNRISEAFLEKKVALTDQLGIAVDWVEAFAFAWL
                     ARQTIHKKPSNLPAVTGAKGERILGAIYYA"
     gene            complement(13471..14817)
                     /locus_tag="PK522_04690"
     CDS             complement(13471..14817)
                     /locus_tag="PK522_04690"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNC41062.1"
                     /translation="MFYTPLPSKTRILAQKAPVVTQKGLRWLTIISSIPLFGIVTAFG
                     IAPTTSSLQDIPVRQIVHSLPLPEIAVTNEDENQYSVSTFWHQENIRRGDTIAAILSR
                     LEVNKQDKADFIRAVRDNKVMQHLRSGEIIHAETSTEGELLTLRYFNIQGKQLNIGKI
                     GSFYEIDNETAETNARIRVGSGIIQNTLFAATDAAGLPNGIAAKIVEIFASDIDFYRD
                     LQKGDRFTVVYESLQDYGSTIQSGRVLAVEFINKGKPYRAVYFQEPGGEGDYYNPNGE
                     NLRKPFLRTPLEFSRISSGFTNARFHPILKQWRAHKGVDYAAPTGTPVMATADGVVEF
                     KGWQNGYGNMIILKHRGEYSTAYGHLSSFGRGLQKERRIKQGEIIGYVGSTGMATGPH
                     LHYELRVNGIQRDPSQVIMPAALSISKRNKAIFQKETKELIARLDLLRNANFASAK"
     gene            14955..>15356
                     /gene="tyrS"
                     /locus_tag="PK522_04695"
     CDS             14955..>15356
                     /gene="tyrS"
                     /locus_tag="PK522_04695"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNC41063.1"
                     /translation="MCSSTNQANEQLKIIKRGCQELLVEEEVIQKLTRDHPLRIKAGF
                     DPTAPDLHLGHTVLLNKLRQFQDLGHHVLFLIGDFTGMIGDPSGKNTTRPPLTREEVT
                     ENAKSYTAQVFKILNPEKTEIVFNSTWMDKLT"
BASE COUNT         4457 a         3232 c         3203 g         4464 t
ORIGIN      
        1 agcagcgagt ggtgcccaag cgagcgttcc tttcgtttca acactcccct gttgattaac
       61 ttgcgcctgg agcgatagct ccacaggctg cgccccgctt aggtcgatat tgcgcactgt
      121 gagattaagc ggattgatat gcattggtac aactttggat accgtgtaat cttcaaacgt
      181 gatagcgcca tcgctcaatt tgatctgatc gatataaatt gcccacggct ggtctgtttc
      241 ttcagccgct ggggcagact gacttgatgt ttcctcagcc atttttccca tgttagcctg
      301 tggttgcttg gtatcttctg gtctagcaac aatctgcggt ttatgttcgg gcaatggaat
      361 ggttataact gatggagcca cgagctcctg ttcacgcgct tgtgtttgtg ctaaagacgt
      421 tacgcggtta tctggttttt tgtcgggaat cggaatgatc gctgaaacgt tgcgctggat
      481 cggcttgctt agtttcttta ctttatcttc ttccgactgc attgaattgg atgatgcagt
      541 taccgcaagc atgcggttta attccagctg accatcgggt tgccgtttga ttaacccatt
      601 aatcccatct aacaatatgg taccgaggtc gattttgtgc cgtgcgcgat cgaccgtgat
      661 gctatctaat tcaatttttg gtaaggatag taaagcttgc ttttctccaa tcggggttat
      721 cgcaatggac tcaaaagtcg ttccaacttt cacaggcttt gtttcattga atgatacttc
      781 attcaacttg agattaaatt gcttaacacc caatacataa ggcatttcaa ccgttctatt
      841 ttcaatctgt attcctttaa gcgcaatttg accggataga tcgatattgg gtggctcatc
      901 gattacatgg gtaaagctaa ctaacaaatc gctatcaaat tttcctgata gtagccggat
      961 ccctaacgga atcggtgaat attcatcgat attcgtcaaa tcaatatcat ccagtttgag
     1021 cgaaagcgtt gcttcttgct tgtcggaaaa aggtagtact ttgccagaca aggtaacagg
     1081 cgcctcgttg attttggcgc tcaggttcgg ctccacccaa tttgtgagga catttttaaa
     1141 attggcaatg aatggaatac tcaaattgat cgcagtaatg tgctgctgac tattcttgat
     1201 tcgatcggca aaggtaatct caccgttttg gatactgatg ttgttgattg aaaatagcgt
     1261 tggttttttg ggctcttgtg gtttttcttc aggcgtttgc ccaaatttct cgattaaatc
     1321 tgagaaattg aactggtttt tatcctccct tgtgacatat attttcgggg caactaatga
     1381 aatagttgtc acaacgggtg ccagatggat gagtgattca gcactaagat ctacatataa
     1441 ttgatcaaac gagaaaaaag tagtttgatc acttaccgtt gatttttccc cgattcggaa
     1501 gcctctcact gcaacttcca gggtaaacgg tttaatatcg atcgattgga cggtgacagg
     1561 ccttgctagc gtttccgaca aaacttgctc gagcttggct ttcgcaaatc caggtaacca
     1621 gaaatatccc aataccccga agagcactac aatcgagaaa atgacgccta agctgattgc
     1681 aaggtgctta cgtgtcagga aacgtttttt tgtaggcgat gatatgtctg tttccataga
     1741 aatcagccat tcccttcatt ttttgcttga tagtgactta ctagccgctt ttgaacggct
     1801 agcttgaatg ccttagttac tcaaaaccaa ccagctcaac atcaaaaata agcgttgcat
     1861 gtggtggaat cacattacca gcgccctgcg cgccataagc catactcgat ggaataatta
     1921 aggtacgctg cccgcctact ttcataccta ttacaccttg gtcccagcct ttgataactc
     1981 gacctgcccc gagcataaat gcgaaaggct ctttgcgatc ataagaacta tcaaatttct
     2041 ttcctttttt atcgggtgca ctttcatcat agagccaacc gctatagtga actttgactg
     2101 ttttaccgac gatggcttct tccccgttac caaccgtggt atcgattttt tgtaatgcac
     2161 ttacaccaga agctgcgcta tgcggctctg ctgagtgctc tttataggca aatgctgaaa
     2221 aagaaaataa catgctgacg attgcaatga aacaacctaa taatttaatc cttaacataa
     2281 gtactcccat ataatccatt cgctaatata tgaataatca actcatcgct gaattcttgg
     2341 ttattataat actggatgag aaatttcagt gcgtcttagc aattgagtcc tcattcatga
     2401 tgactggcaa gcgcaatcca actgaatgga ttaggcagga acgtatcaaa accacatcgt
     2461 ttcaaatctt catcgttcca gacggaccga tgattctcga aaggatttgc aggaacatcc
     2521 tgctccatga attccttagg cgtgctaatg agcgatgaac ccttcgcgat acgtcgacat
     2581 tcttttaaga aatgaacacc ttgctcctta taaaaatgtt ccaagatatc gattgcaaga
     2641 actaagtcat agctattgct ggcgagtgtt ggaagtaact ccagcgcgtc accaattaga
     2701 atgcggttgt aggcataatt atgcacaggc gtgagataac ccgcataacc ttcgatacca
     2761 tcaattttaa ttctccactg atttttatcg cgcaatcgag cattcgcacc attgatttca
     2821 aaaagattga tattttccag attggtgcgc agtagaaaac catactgacc catgccaacc
     2881 ccgatatcca gaattgatag tggcttttgt tgttcagcat agccaataat ggcgctaagt
     2941 tgattactaa aactaaatgg catcgtttct cctgtttaga gggtgggtct tcttaatatg
     3001 agtacaatta aaaacaaaag catagcaaga ataatattaa ttgggtgata gctcgattta
     3061 tcttccaatt aattaactcc cgatcatcgt atacttttca agtttcgttt atccataagg
     3121 agaagaaggg tgagtcagca atcaaatagt agttcgatca gcacggatga gagaatcata
     3181 tcaggttcaa atggctgggt aatgttagtg tcactactcg ctctatttgc ttttagtctt
     3241 ttctttacca tgacaccagg ccctcccata aaattgtttg gtggaatttt gttagcaatg
     3301 ctcgctttgt ttggatgcaa aggattattt acattagagc ctaatcaagc tgccgtcatg
     3361 gtattttttg gcaattatgt tggaaccgtg aatgaaagcg gttttttctg ggtcaatcct
     3421 ttttataata aaacgcgggt ttcgctcaga ataaataatt ggaacacccc tgttcttaaa
     3481 gttaatgatg aacgcggcag tcccattgag atcgcggctg tcatcgcgtg gcgtgtccag
     3541 gatactgcac gcgctatttt tgatgtcgaa agcacgttga attatctgca aatccaaagc
     3601 gagtctgctg ttcgccaagt tgcaagtagc catgcctatg acgatactga aatgatcgac
     3661 ggtaagcgta aaaaaagtct gcgaacagac ttagacgcca tcgcgggttt attacgagaa
     3721 tcgattcaag agcatgtcaa cgtggctgga atcgttattg aagaagccaa gattgctcat
     3781 cttgcttacg cccctgaaat cgccaatgcc atgttgcggc gacagcaagc agaagcggtt
     3841 gtgatggcac gtcagaagtt agtggatggc gccgttggaa tggttgaggt ggcattgaag
     3901 aatcttgaag aaaagcaaat tgtctctctg acttcggatc aacgtgctgt gctcgtaacc
     3961 aacatgatga cggttctgct ttctgaatca ggtgctcaac ccgtgattca aatgacacaa
     4021 acaactccat agctagaaat tggtagaatg cccgaaggtt aattccaatt ctgacaagat
     4081 gacacatgct ttaaaattat ggcccctttt gaccggaacg attcgttatg aaaaaacgat
     4141 ttcaacccac aaccggggcc acgggcagtg gattgatgca cccattctag cttatttgat
     4201 cgaaactact caggggcgca ttttgtatga tgtaggttgt gattatcaca agctttctga
     4261 tcctgctctg cgtcagcagt tttttgcgag tatgtatcca gttatagaaa cccctcaaat
     4321 gcaagcagaa caacgaattc cccactacct tgaacgattg ggattaacga ctgctgatat
     4381 tgatctcatt ttgattgggc atctccattt tgatcatgtt ggcggtttat gcgatttacc
     4441 cggctgcgaa gtgcatcttc accaagctga acttgcagca gcccgaacca aacaagattc
     4501 aggcgttttt cccgatgagt tattgcaatc tgatcgatgg cgattacagc atgaagaata
     4561 tgaattagca cctggtgtgc aagtattaac aacacctggc catactgcgg gacacttgtc
     4621 gttgatgatt gagttacccc aaggtcaacc tatcattcta tgcggtgatg cagccgattt
     4681 gaatgaaaat ttaaccgatg aaattgcgcc gggttgttgc tggcaagata attttgaact
     4741 ggccttagcc agtatacgca agctcaaatc tatcgctatt tctgagcaag cacaattgtg
     4801 gcctaatcac gatatggttt ttttcaagca attaccggtt tttccaacag ggcatgaata
     4861 agcgctgccc cttctactta aaccttttat cgatttacaa aaacagattc taagaatttg
     4921 attttttggt agttgagtct aaagtagttt tcgttatata ctgaaaccgt cacaagactt
     4981 tttgttacat tatttaaaat aaggaaatta aggatgaaat acattattat cgctgcggcc
     5041 ttagcaggtt tatcgttagc cggatgtggt ggcaaaccaa gccaagctct tcctacgact
     5101 gaaaaagaag cttatgaaaa aatcatttct gggcaaagtc ttgattgtca atttgggatt
     5161 gatgcgaatg gtaattgcct gaaagaaggt gaagatcctc gcccatatgg tggcaaaggc
     5221 aaacctggtc attaatgcag tgattactta gcttcctgaa gcaaaaggaa gttatgaagt
     5281 aacctaaacc gcccttgagg cggtttttat ttttgttgtt ttcatttctt gcctaaagta
     5341 ataatttcta ctttatttat tctaggcact gcgtttctaa atctctagca atctaaaatc
     5401 agcaattttt ttgcttgatc aaaaaattta ttgaatacga ataacttttt tgagtacaat
     5461 aggtaaatca taaattacca gtcacatact atatatagta catacctggg tttttgataa
     5521 gcaaagcggt cagtatgtct ctctatctgc gactcaaagg agaattcgaa tatgcagctt
     5581 acggctaata gtacaagcac ggtcttaaac ccgattcctc atggtgagtt ttctgaaggt
     5641 tcttccaatc aaggtgtaca actttctctt tataaaataa tccggcgcaa tggcgctgtt
     5701 gtggcatttg aacccagtaa aatttctgtt gcgatgacaa aagctttcat cgctgttgaa
     5761 ggaggtcaag gtgctgcttc tgctcgagta agggaagtag tagctcgatt aactgaagaa
     5821 gttgtcaatg cattgacaag acgtcaatct ggcggtggca cctttcatat tgaagacatt
     5881 caagatcaag tagaattagc tttgatgcgg tcaggtgagc atgatgttgc gcgttcttat
     5941 gtcttatatc gtgaagagcg agcccgcgaa cgtgcaaaac agcaagaaaa acaaactaca
     6001 gccaaaacgc cgcttattca cattgtagaa aatgggcagc gcattccgct cgataccaaa
     6061 caattaacgg aattagttga tgcagcttgt gccgatttgg gcaatagcgt tgatagctca
     6121 ctgattctta aatccacgct taaagatctg tatgatggcg tacctgctaa cgaagttaga
     6181 aaatcgctga tattatcggc acgtgcgctg attgaaaaag atcctgatta tagctttgtg
     6241 acagctcgat tgctgctgca ttctattcga ctcgaagcgc taggtgagaa tatttcccag
     6301 aaagaaatgg aatcacgcta cgctgattat ttccctaatt ttattaaaca aggcattgat
     6361 gccggtttgc tcgatgagcg cttagcgcaa tttgatttga ctcaattggc agctgagttg
     6421 gttgcaagtc gagatatgca gttcggctac ctgggtttgc agactttgta tgatcgttat
     6481 tttctgcata taaacgataa gcgtattgaa ctgccacaag cctttttcat gcgagtagcg
     6541 atgggtctcg cactcaatga aattgatcgg gaagcccgtg caatcgagtt ttatcatgtt
     6601 ttgtcaaaat tcgatttcat gagctcgaca cctacactat tcaattcggg aacttgtcgt
     6661 cctcagcttt cttcctgtta tctgacaaca gtgccggata gcctggatag tatttacgaa
     6721 gcgataaagg aaaatgcgtt actttcaaaa tttgcaggtg gattaggaaa cgactggaca
     6781 ccggttcgcg caatgggctc gcggattaaa ggaaccaatg gcaagtcaca aggtgtagtt
     6841 ccttttctta aagtagtttc agatactgcg gttgccgtca atcaaggagg aaaacgtaaa
     6901 ggggcagtct gtgcctatct agaatgctgg catctcgata tcgaagagtt tcttgaattg
     6961 cgcaaaaata ccggtgatga tcgccgccgt acacatgaca tgaatacggc cgtttggata
     7021 ccagatttat tcatgaaacg ggtaatggag aatgcggatt ggacattgtt ttccccttca
     7081 gatacccctg atctccatga aaaatatggt aaagcctttg aaacagccta tttgagttat
     7141 gaagagaagg cgtcgcgcgg tgaattatcc ttatataagc aagtttctgc gcagcaactg
     7201 tggcgcaaaa tgctaacgat gctgtttgaa actgggcatc cttggattac gtttaaagat
     7261 ccctgtaata tccgttctcc gcaaagccat gtgggcgttg tccatagctc taatctctgc
     7321 accgagatta cactgaatac gagtgataaa gaaattgcag tatgtaatct aggatccgtc
     7381 aacctactcg cgcatatcaa agatagtaag cttgataccg aaaaactcaa gcgcacgatt
     7441 agcactgcga tgcggatgct tgataatgtg attgatatca atttttatgc tgtcactaaa
     7501 gcgcgtaatg ctaacctcaa acatcgcccg gttggactcg gcatcatggg ttttcatgac
     7561 tgtttgtatc aacttggtat ttcttacgct tcgcaagaag cgatagaatt cgctgatcgt
     7621 tcgatggaag cagtcgcttt ccatgcttat tgggcgtcga ctgagttggc ggaagagcgc
     7681 ggttgctatg caacttatcg taatagcctt tgggatcgcg gtatcttgcc tcaggatacg
     7741 cttgatttgc ttcgtgatga gcgaggtggc tttgttgatg tggatacgac tacaacatta
     7801 ccatgggaca aactgcgggc acgcataaaa aaacatggca tgcgcaattc aaactgctta
     7861 gcaattgcac caactgccac aatttccaat attatcggcg tatcagcaag cattgaaccg
     7921 acttaccaaa atctatacgt caaatcgaat ttatcaggag aattcaccat cgccaatcga
     7981 tggctagtca acgatctcaa gaaacatgaa ctttgggatg aagtcatgat tgcagatctg
     8041 aaatactatg atggtgcaat tggtaagatt gatcgaattc caccgctaat tcgtagtcaa
     8101 tatgcgactg ccttcgaggt tgatccgtac tggttgattg aatctgcagc tcgacggcag
     8161 aaatggattg atcaagcgca atctctaaat atttacattg cgggggtatc cggtaaaaaa
     8221 ttagatgaaa tttataaatt ggcttggctg agaggactca aaactactta ttacttacgt
     8281 tccttaggag caaccaccgc tgagaaatca actgtctata caggcgcgct caatgcagta
     8341 ccgactagtg cgggagcaac catggctaat gcacaacaaa cagcagccac tgagattaaa
     8401 tattgtgcga ttgacgatcc aggttgcgag tcttgtcaat aaagcgtatg tgcttataaa
     8461 taaaacatag aaggagtaat aacaatgctg acatttgatg atgagccact gcaatcagaa
     8521 gcgattcaag gcaaatcgat cttaagtaat agcagaccgg attgggctga atcgactgga
     8581 attacccctg ttgcaccgcc tcctagcacc atctctcttc cacagaatga acagttggca
     8641 gaagatatcg cgttgagacg ggtctccgtg gaagataaac gcattattaa ctgcaagacg
     8701 gatgttaacc aactcgttcc tttcaaatat aaatgggctt gggaaaagta tttatctgcc
     8761 tgcgcaaatc actggatgcc gcaagaaatt agcatgagcc gtgatattgc actctggaag
     8821 gatcccaatg ggctaactga agacgaacgg cgattagtga agcgtaatct gggcttcttt
     8881 gttactgcag attctcttgc agccaacaat atcgtactgg gtacttatcg tcatatcact
     8941 aatccagagt gccgccaata tttattgcgc caggcttttg aggaggcaat tcatacccat
     9001 gcctaccaat atatcgttga atcgctcggt ttagatgaag gtgaaatttt taacgcttat
     9061 catgaaattc agtcaattcg cgataaagat gaatttctaa ttccttttat tgatactcta
     9121 accgatccaa aatttaaaac aggtacgcta gagactgatc aacagctgtt gaagagtcta
     9181 attgtattcg cttgtattat ggaaggtttg tttttttatg taggcttcac acaaatttta
     9241 gctctaggta ggcaaaataa gatgatgggt gctgccgagc agtatcaata tattttacgc
     9301 gatgagtcga tgcattgtaa tttcggtatt gatgtcatca atcaaattaa attagaaaat
     9361 ccgcatcttt ggaccaaaga atttcgtgaa gaaattacca ctttgatgca caaagctgta
     9421 gaattggaat atcgttatgc tgaagataca atgcctagag gcgtattggg attgaacgca
     9481 gcgatgttta aagaatatct acgttttatt gccaatcggc gttgccagca aattggctta
     9541 gatgcactct tcccagaggc aagcaatcca tttccttgga tgtcggagat gattgatctt
     9601 aaaaaggaga aaaatttctt tgaaacacgc gtcatcgaat atcaaaccgg tggtgtattg
     9661 agttgggatt agattgataa atctcaatct ggcctagcca aaattaacgc tacctacttt
     9721 tagggacctt aaaataatca tgaattggca ctgcatcgtt gtgtagtgcc tttctgctga
     9781 ataccacttc gcgtttttgt aaggccgtat atttctattt aacgcactgc gttatcaagt
     9841 ggtttttcta tcagcagatg taactgaatc tgaattttca gaagtttgac tatttttgaa
     9901 aattaaggat gaacatactt atgctaaaaa taaaaaaaat tcaaatcctg atgcttagta
     9961 tcgcaattag ctcagtattg ctattactac catatcaagt tgaagcagct agcacggcgc
    10021 aaactaagaa aaatacttat gcagcaaaag gaagaatagc ttcagcaaat agaaataaca
    10081 atgtaggcga tctatggacc agagtccgaa gcggattctc tatgtctgag ctacaaggac
    10141 atgaagtgcg ccagaatgaa aatggctata ctcgtcacca aaaatttatc gaccatatgg
    10201 ttgagcagag taggcggtat cttttccata tcgtagaaga agtcgaacgg agaaaaatgc
    10261 ctactgaaat cgcactatta ccgatcattg aaagtgcatt taatcccgtt gcatattctc
    10321 atcgccatgc ctcaggtctc tggcaatttg ttcccgcgac cggtcgtgct tatgggttag
    10381 agcagaactg gtggcacgat gatcgtcgtg atgtaatcgc tgcaactaat gctgcactcg
    10441 attatctcca aaaactatac cgaatgtttg gggactggaa actgacggtt gctgcctata
    10501 attgggggga aggtgcgctc aaacgcagta tcgatgaaaa tcatggcggg gatttaaaag
    10561 ttagcttcca cgatctccaa ttacccacgg aaacaaagaa ccatgtagca aagctaattg
    10621 caattagaaa tatcatagcc aaccctagcc aatatggcgt caaacttaaa ccgctcccca
    10681 atcgacctta ctttaaacaa gtaaaaatca cgcagcatat ggatgttaaa cttgctgcgg
    10741 agctggcaga ccttccagag catgaattta atgcactcaa cccagcgtat catcgaccgg
    10801 ttattcgcat caaagataag ccacgcacac tattacttcc agttggtagt gttaaaacct
    10861 tcgaagtgaa tttagaaaac tatgataaat cgcttgtttc ctggcgcgtc tatcgtgtta
    10921 aagaaaatga aaccttagat gatttatcca tcaagtatcg agtcgatatt gcagagctag
    10981 cacaagtcaa tggtctctct gaatacgata cgattgaaga aggacagacg ttgcttgtgc
    11041 cacgcaaaag aaatagaaac agcaatgatg atcgcctatt tgtagcacaa cccaccttca
    11101 gcgagttaca catgcaacca gtgaaggtat cccctattat tcatattgta caaaaagggg
    11161 atacactgta cgacatcgca aagcgttata gcgtagacgt aagggaactt aaatcatgga
    11221 atggaaataa agaacggctt gcaattgggc agaaattaat cgtgaggcta gccgactcga
    11281 atagaaatca atcggtcact aaaccctctt gactactcaa actctgatat ttttttgaat
    11341 ctaaagtaca aacgaacaaa agcggaaaga ttaccgagcc ggtcacaatt taaatatcag
    11401 gctgcaagta gccttcccac gtagcgttct ctgatgttaa ttagtctaat gctactacaa
    11461 acaagcaatc aaacgaaatg gggtctccca catcacctga aaaatttgcc atgctaacca
    11521 tgccagatag ccttgctgtt cctttcgcgt gagcaaagcg acctgtacca ccaacaatcg
    11581 cgtttccgtc actggatgca cccgtaatat gagtaatcac ctgaccgcgg ggtgttacag
    11641 ttggatggaa aacaggttga actgaggttt ttccacgtgt aattaaagta ccgcccggta
    11701 aatggaaata agtagtgcca actaatgcaa cccccgttcc ggttggcgtc accatcgcca
    11761 aacaatcggt acctttgcct aacgctcggt ttgtatgcgc atcaaaaacc tgaacatcga
    11821 aacacatagc cagatcatct tgcccatcgc catcaatatc aggaaccata ttctcgtaca
    11881 ttgttcctgt tcctacgaga tttagggcaa gatgcttgcc tgcattatct gacttatttt
    11941 ctgcctgaag tgaaaatgat aataataatg cacagccaac taaaatcgag cttcctttta
    12001 aattcaacat atcaataatc tctcctatca aaaattaatc aacaacaccc catatctagc
    12061 ttcttgttgt gttttatttc taaaaatcac ctcaagaagc taaatttcaa attattttgc
    12121 aggatattga tttaggtatt gatacgatgt gatttttatc acggcaagat ttgtaatctt
    12181 ttgattctgg tcatgccaca aacttaactg aagcataatt tcttacacct taaatgaatc
    12241 aatttcatat caagtgctgc tatttagcgt tttaaagaaa gaaataccgc tataagatct
    12301 tgtggctcta atatttttag gactaactag gcatagtaaa ttgcacctag aatacgttct
    12361 cctttggcac cagttacagc aggtagattg ctaggttttt tgtggattgt ttgtcgtgca
    12421 agccaggcaa aagcaaaagc ttctacccaa tcaacagcga ttcctaattg atcagtcagt
    12481 gcaactttct tttccagaaa tgcttcactt attcgattca caagccttgc attatgcgct
    12541 cccccgccgc atagataaat ttctgttgca gcaggaaagt aagattgtat gctccgcact
    12601 atgctttgta ccgtcaattc gagtaaggtt gcttgcacat ctacaggaag ctcagttccg
    12661 tttaaatgca ttctcagcca atcaagatta aagcaatccc taccagtgct tttgggggga
    12721 ggtaaagaaa agaaaggata cgataataat gcttctagca acagcgaatg cgattctccc
    12781 ttacttgccc agttaccatt atcatcatag ctttgtcctt ggttctcaaa gcaccaagca
    12841 tccattagca tattgccagg gccacaatca aaaccaatta caatatcttc aggtgaaaga
    12901 cttgtaatat ttgcaatacc gccaatattc acgataatcc gatgagatgt ttgatgacca
    12961 aaacaagcct gatggaatgc gggaaccagt ggtgcgcctt ggcctcctgc agcaatatca
    13021 cgactcctaa aatcagcaac gacagtaata ccagttaatt ctgccaataa cgctggatta
    13081 aacaattgga tgctataacc agaatctgga cagtgtctta tggtctgacc atggcaaccc
    13141 attgctataa tcgattctga gctgatctcc gctgcgatca gtaaattatt tattgcttgt
    13201 gcgtatgaac ttgataatac attagcgagt aatgcagaac ggcgcaattc attctcgcct
    13261 tgcaagtgca acgccaataa atccttcttt aaactagcgc aatagggtaa gaaataggtc
    13321 tttacaagct tgggtggact gagcctaaaa tctactaata caacatcaat accatcaagg
    13381 cttgtgccag acattactcc tacatacagc tcttggccca atggctggat tcccaaatgc
    13441 taatctaatt gggtaccgac gattcctatg ctactttgct gacgcaaagt ttgcattgcg
    13501 aagtaaatca aggcgagcaa taagttcttt agtctctttc tgaaatatag ctttatttct
    13561 tttactgatt gatagcgcag caggcataat aacttgagat ggatcgcgct gaatcccgtt
    13621 cacccgaagt tcatagtgaa gatgagggcc ggtagccatc ccggtcgaac caacatatcc
    13681 gatgatttcc ccttgcttaa ttcttctttc cttttgcaac cctctaccaa atgaagacaa
    13741 atgaccataa gcagtactgt attcaccacg atgttttaga atgatcatat tgccataccc
    13801 attctgccag cccttaaact cgacaacacc atctgctgta gccatcactg gagtgcctgt
    13861 tggtgcagca taatcaaccc ctttatgggc ccgccactgc tttaagattg gatgaaaacg
    13921 ggcattggta aatcctgagc taatacgtga aaactcaagt ggagttcgta aaaatggctt
    13981 tctgagattc tcaccattgg gattatagta gtctccttct ccaccgggtt cttgaaaata
    14041 aactgcgcga taaggtttcc ctttgttaat aaattctact gccagaacgc ggcctgattg
    14101 aatcgttgat ccataatcct gaagagattc ataaacgacg gtaaaccgat cccctttttg
    14161 tagatctcgg taaaaatcga tatcggaagc aaaaatctcg actattttcg ctgcaatgcc
    14221 attcggtaat cccgcagcat ccgttgctgc aaataaggta ttttgaatga tgcccgatcc
    14281 taccctaatg cgtgcattag tttctgctgt ttcattatcg atttcataga aactaccgat
    14341 ttttccaata tttagttgct taccctgtat attgaaatat cgtaaggtta atagctctcc
    14401 ctctgtcgaa gtttcagcat gaatgatctc tccagatctt aaatgctgca tgactttatt
    14461 gtccctaaca gcacgtataa aatctgcttt atcttgcttg tttacttcaa gcctggaaag
    14521 gatagctgct atggtatctc cccgacgtat attttcttgg tgccagaaag tgcttacact
    14581 atattgattt tcgtcttcat tggtaacagc gatctcaggc agcggaagcg agtggacaat
    14641 ttgacgaact ggaatatctt gcaaagaaga tgtcgttgga gcaattccaa atgcggttac
    14701 gataccaaat aaaggaatac tcgaaataat ggtcaaccaa cgtaaacctt tttgtgttac
    14761 gactggtgct ttttgcgcta gaatccttgt tttgctaggt aatggcgtat aaaacatact
    14821 acgtatcact cctatatatt gtgagttgcg ctaagtctag cataaaaaat ggcctgcgcc
    14881 ctagcataac tcgttagcgc tgaataggtt tctcaaccct tttctcttaa aacttaatca
    14941 cttagataaa aatcgtgtgt tcctcaacta atcaagccaa tgagcaatta aaaattatca
    15001 aacggggttg ccaagaatta ttggtcgaag aagaagttat tcaaaaactc acccgtgatc
    15061 atccgctcag gatcaaagct ggtttcgatc ctacagcgcc tgatttgcat ctggggcata
    15121 cggtattgct caacaagctt cgtcaattcc aagatttggg ccaccatgtt ttatttctaa
    15181 tcggggattt cactggaatg attggtgatc ccagtggcaa gaatacgacc cgcccccctt
    15241 taacgaggga agaggtgacc gagaatgcaa aatcttacac tgcccaagtt tttaaaattc
    15301 ttaatccaga aaaaactgaa attgtcttca attccacttg gatggataaa ttaact
//
LOCUS       DAOQLJ010000043        15203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1421_length_15203_cov_6.317732, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000043 DAOQLJ010000000
VERSION     DAOQLJ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 15203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15203
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1421_length_15203_cov_6.317732"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PK522_04700"
     CDS             <1..248
                     /locus_tag="PK522_04700"
                     /inference="COORDINATES: protein motif:HMM:NF022748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HNC41064.1"
                     /translation="WDGMEPALKKKWIRITKHYLKRTPQEQQRIQSQMQNWSQLTTEQ
                     RKLARDRYKKLENLPPGKLLPIIEKWRKTEEIPKNSQ"
     gene            313..2571
                     /locus_tag="PK522_04705"
     CDS             313..2571
                     /locus_tag="PK522_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC41065.1"
                     /translation="MSPLPPLWNEQVHPQLAKNETIVAWLELDLTDQLFFSKGILIVS
                     NRRLLSKMQNEEHWLSFVYQKELELTTHDHAGVGHIELFDTQSRLAHWCYTLGKLHFV
                     HHLVECFNQQSNFHLEGFPENLVTDSTYDEIGSTDKNSTSIEEQTSRETVSTFTLLRL
                     WRFAKPYRGRLLIGFLLMLASTAATLVPPYLTMPLMDEVLIPYQNGYPLNVDLATLYL
                     GGLLVAAILAWLLGWSKTYILAKVSERMGADLRITTYNHLLKLSHEYFGTKRTGDLMA
                     RIGAETDRINLFLSLHLLDFATDVIMMIMTAMILVSINPWLALVTLLPLPFIAWLIHL
                     VRDKLRTGFEKVDRVWADVNNVLADTIPGIRVVKAFAQEEREVKRFYDVNQRNLEIND
                     RVNKVWSLFTPTVTLLTEIGLLIVWIFGIWQVSENQITVGVLTAFLAYIGRFYLRLDS
                     MSRMVSITQKAAAGTKRIFEILDHVSRVPEPVKPIHSPTIQGHIELRHVGFRYGVRRI
                     TEDINLIIEPGEMIGLVGHSGSGKSTLINLICRFYDVTEGVILVDGIDIRSFPIAEYR
                     KHIGLVLQEPFLFFGTIAENIAYGKPHATRQEIVAAARAAHAHEFILKQLHGYDSLVG
                     ERGQALSGGERQRISIARALLINPRILILDEATSSVDSQTEQEIQAALDNLVRGRTTI
                     AIAHRLSTLRQANRLIVLDQGKIIEVGSHATLMAKRGYYYQLYLSQTGHIGVDPSLID
                     SNRESNQTKEWI"
     gene            2568..3041
                     /locus_tag="PK522_04710"
     CDS             2568..3041
                     /locus_tag="PK522_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HNC41066.1"
                     /translation="MNYTKFKLSRDRHRKLILFDDAYQQKSHQVIAIRAFPISAADKG
                     IALVDHQGNELMWIENLQDLPVEWRELIEEELTSRDFMPEIKKIDSVSSFITPCVWWV
                     ETNRGNTCFTLKGEEAIRRLSSLALLITDQYGVHFLIKDRTKLDHYSIKLLDRFL"
     gene            3135..5423
                     /gene="cphA"
                     /locus_tag="PK522_04715"
     CDS             3135..5423
                     /gene="cphA"
                     /locus_tag="PK522_04715"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNC41067.1"
                     /translation="MHSNYTNRQFDLKFLEIIHLNGPNRWTYYPALEALVDIGALEDY
                     PSHIIPGFYDRLAAWLPSLIEHRCSYEERGGFLQRVREGTWPCHILEHVTLELQNLAG
                     MKGGFGRARETAERGVYWVVVSAWHAEITKQALFAARELILAAMGFEARQVDYDVQAA
                     IAQLRDMVDSLWLGPSTACIVDAATERNIPAIRLLSKGNLVQLGYGSNSRHIWTAETD
                     RTSAIAENISRDKELTKSLLRACGIPVPEGRITHSPEDAWQAAEALGLPVVIKPCDGN
                     HGRGVFIELSQCEEIKSAYSVALKEGSGVLVERFVSGFEHRLLVVNGELIAATRGDMI
                     SVVGDGISCIAELIEQQINSDSRRGTSEEHPLNLIRLDSAAQLEIAHQGYSADSVIPV
                     NARVLIQRNGNHAFDVTDEVHPSTAKIAALAARVIGLDIAGIDLVVEDISKPLLEQSG
                     AIVEVNAGPSLLMHIKPAVGKSRPVGKAIVEHLFPEKGNGRIPVIGITGSYGKTTVAH
                     LLTKLISLSGKRVGLASSKGLFFEHKRVDERNSANWSSANRVLMNRMIDVAVLENSFN
                     SLLRQGLAYDSCQVGIITNIDPNLHYGFYGIATPEQVYAVMRTQVDVVTPTAAAIDDV
                     IKEITLPMGSAILNARDSMLVTMSQLCHGEVIYFSVDPDLPSIKQHRIEGSGSASRKR
                     AVLVRDGSILLVDGTNEMVLIQLTELLPPTDSECIVVEIENVLAAIAAAWALDISPDV
                     MRVGIAAFGSSQQEAPCQSQFS"
     gene            5449..8025
                     /gene="cphA"
                     /locus_tag="PK522_04720"
     CDS             5449..8025
                     /gene="cphA"
                     /locus_tag="PK522_04720"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNC41068.1"
                     /translation="MKLIQSRVLRGPNLWSKHPSIEALLLFDEGACLIEDIPEFESRL
                     RDRFPEIALQYPANSHDVRTIAQVLEFAVLGLQNQIGCPVTFGQTISMPDKRNYRVIV
                     EYSEEAVGQLAFQYAHDLCIAAIENLPFNLNHALQHMRTLYEDIRLGPSTGAIVKAAI
                     ARGIPYRRLTDGSLVQFGWGSKQRRIQGTESDATNAIAESIVQDKQLTKALLQAAGIP
                     VPLGRCVMNADDAWVAACEIGLPVVIKPKDGNHGRGVAVNLMTEQQVRLAFTLATAAE
                     DEVIVEQYVTGSDYRLLVVGEKVIAAARRDPPQVIGNGVHTISQLVEQINRDPLRSEG
                     HSNLLSKIHLDQISLDYLATQGYSAESIPNKGVRVILRNNANLSTGGTATDVTDDVHP
                     EIFATAIAAAKMTGLDICGIDMICDSIRAPLEEQRGAVIEVNAAPGLRMHLQPSFGKG
                     RAVGESIINYMFAPGENGRIPIVAVTGTNGKTTTVRLITNILSIHGMQVGMTCTDGIF
                     VDSDGIDTGDCSGPKSARNVLFHPKVDAAVLETARGGMLREGLGFDHCDVAVVTNIGR
                     GDHLGLAHINTSEEVALVKRIVIENVLPKRGVAILNADDPLVVKMADHSPGSVIFFTQ
                     NSRNPVMLTHRIQQKRVIYLDKKTIIASSIDTEQRFALSEIPLTQNGLIGFQIQNVMA
                     AIGAGWALGIDWATIRAGLANFVSDVQTVPGRFNFFNYRNATLIADYGHNPDAIEALV
                     DAIDNLPAKKRIVVISAAGDRRNEDIRLQTQILGDVFDEIVLYQDQCQRGRSDGEVLG
                     LLREGLEHAKRVSKVREIHGEFKAIDTALTNLQAGELCLILVDQVEQSLNHIRERVTS
                     HR"
     gene            complement(8050..8526)
                     /locus_tag="PK522_04725"
     CDS             complement(8050..8526)
                     /locus_tag="PK522_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41069.1"
                     /translation="MKKIIISTLICFFVLPLNAANETDNRKALLLNEAQRHHILSEMR
                     SMLLGTQQIIQALAEDDMLAVARYARMLGMEMAHKGEDHLQSVLPDDFMQLGMSVHRS
                     FDKIATDAETLKDSRHTLSQLSMTLQQCVACHSAYQIWTTDIPSDAEPHPTHHLSH"
     gene            complement(8567..10954)
                     /locus_tag="PK522_04730"
     CDS             complement(8567..10954)
                     /locus_tag="PK522_04730"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNC41070.1"
                     /translation="MTHSIINKNKLFLGLAFFLLGLGGIFWFVLSASLPQYSGSAKLS
                     SLTQAVRVDRDALGTATIQAMNRLDLTQALGYVHAQERFFEMDLMRRSAAGELAELFG
                     KAALPKDRQIRKHRMRTRAKHMFDNLPEDQRALIESYRKGVNAGLATLLIQSFPYLLI
                     RERPVPWQNEDTLLVILAMYFTLQESSLNRELQLSALQTVLPASVYQFLTASGGDWDA
                     PLIGIPLKWPQLPSKEEFNFQAYAHLPTMEQDRLELHDEAPGSNSFAVSGSLTDGAAL
                     IANDMHLTLRVPNIWFRTRLIYPHPNKAGINVAVSGVTLPGVPAIVVGSNGNVAWSFT
                     NSYIDVADWVRVVRHPEHSTRYLSDGDWKSLATFQEILHVRDAEDEFLEVEETEWGPI
                     LGHDHDGTALALKWVAQHRDAADLKLMRLEWVETAHQALEIAQQSGIPAQNFIVGDRE
                     GSIGWTIAGRIPMRSGNYDPNFPSDWPNGETGWLGWLPSAHYPLISNPMNMRLWSANA
                     RMIDGLALVKLGDGGYELGARSKQIRDNLFAKEKFTALDLFEVQLDHRALFLSRWQKL
                     LHEVIDHAPIEPWKQKLEDGLLDWDGLAVPSSVSYRIVRTFRQEVVDSIYRNFIAVVK
                     HQYPDFSPPRLPQIEHAIWRLIENRPPHLLPLGNANWEAFLQKCARKVALLMETQSGD
                     DKPWNWGGQNTAQIQHPLSKVLPNFVAWWLNMPKDLLPGDSFMPRIQAPSFGASERFV
                     VAPGNEEQGYFSMPGGQSGHPLSPYYGSGHQDWVSGKPTPFLPGIAQQTLWLAPH"
     gene            complement(10965..11462)
                     /locus_tag="PK522_04735"
     CDS             complement(10965..11462)
                     /locus_tag="PK522_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HNC41071.1"
                     /translation="MIALKWFLVLILILVIVAILAGQLGMFKGGAPNDLGVVEGRLKL
                     PADTPNSVSSQADLYPNHPQHNYARIEPLPLVGDSKSTLSKIKAIIELMPGSQIITNQ
                     ENYLYVQFTTRVMKFVDDAEFWFDPQSNVVQVRSASRLGKSDLGVNRQRIEWIRKQLE
                     EISLK"
     gene            11513..12373
                     /locus_tag="PK522_04740"
     CDS             11513..12373
                     /locus_tag="PK522_04740"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNC41072.1"
                     /translation="MPESRNNLSTQLIQDPNELPLIFHSRVNEPISNLSLLRKSLLFA
                     ELSMIAYNDSNEAKRAVNLIGFPVSIFFDYDGAQGFFFENDHDAVIVCRGTEPNEWND
                     IHADANLGTVLAETAGRVHRGFKQEADDLWPMMEKHLIDLGKTLWFTGHSLGAAMATV
                     CAYRCHVSNKCASPTELYTFGSPRVGNKRYVNFVKLKHYRWVNNNDIVTRVPPIWLGY
                     RHIGIEMYLNRNGLLRTLDYATKRLDRWHGFLRGIRKFRIDHFSDHSIHEYVNHILKA
                     VQKDENNAAS"
     gene            12555..13784
                     /locus_tag="PK522_04745"
     CDS             12555..13784
                     /locus_tag="PK522_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase, exosortase A
                     system-associated"
                     /protein_id="HNC41073.1"
                     /translation="MRILHILDHSIPLHSGYTFRTRSILKAQRALGWETFHLTSTKQG
                     LTNSLEEVIDNFHFYRTPVNNRFINTLPIINQIAVMTSLTQRLNEVVDIVKPDILHAH
                     SPVLNAIPALYVGNKKGIPVVYEVRAFWEDAAVDHGTSNAGGLRYRLTHGLETYALKR
                     ANAITTICEGLRNDIVERGIEAHKVTTIPNAVDLAQFSMNHSIDQSLKQRLGLQDKRV
                     IGFIGSFYAYEGLEILLRSLPNLLPIYPNLQLLLVGGGPQENFLKQTAQTLGIQDKVI
                     FTGRVPHEQVEKYYDLIEVLVYPRLSMRLTDLVTPLKPLEAMAQGKLFIASDVGGHRE
                     LIRDGETGLLFKSGDPDALAKKIIYLFNTPDLWKSLIQAGRAYVENERNWQNSVARYK
                     TVYNQLLVEQGKTVESL"
     gene            13771..14871
                     /locus_tag="PK522_04750"
     CDS             13771..14871
                     /locus_tag="PK522_04750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC41074.1"
                     /translation="MNHYKMHIGLVGPLPPPSGGMANQTRQLAHLLTDENIQVTLIQV
                     NAPYYPQWIEKFKGIRAFFRLIPYLYKLWQASHSVQIFHVMANSGWSWHLFSAPVIWI
                     AYLRNKPVIINYRGGEAEAFLNKSIFWVRPSLKRVSAIIVPSAFLKHVFNQQGFDVTI
                     VPNIIDLDRFAAQTQEWNVPKIENSPHIILTRNLESIYDNATAIRAFSIVKKNFPNAK
                     LSIAGSGPEKLMLEQLVDDLNLVDSVTFLGRVDNTAINDLYRQADVMINTSLIDNMPI
                     SILEALACKVPVVSTNVGGIPFLVKHDETALLVPPKNPGEIAEAIQHILTNPEKSRKM
                     AEAGYEMVQHYAWKNVRTQLMEIYERTLSHRF"
     gene            14896..>15203
                     /locus_tag="PK522_04755"
     CDS             14896..>15203
                     /locus_tag="PK522_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HNC41075.1"
                     /translation="MSTNPVNKHLYTWFIANICFPIHEFFKRHHTVSIRRELEITQWW
                     NKSQIEQLQLSKLKQLLIHAKANVPYYQKIMRDLAIEPEQFESLAALSKLPLLNKAAI
                     "
BASE COUNT         4483 a         3213 c         3238 g         4269 t
ORIGIN      
        1 agtgggatgg aatggaacct gcgctgaaaa agaaatggat aagaataacc aagcactatc
       61 tcaaaagaac accgcaagag cagcaacgaa ttcaatcaca aatgcaaaac tggagtcagt
      121 taactaccga gcaaagaaaa ctagctagag atcgctacaa aaaattagaa aatttaccgc
      181 cgggtaaatt actaccgatt attgagaaat ggagaaaaac agaagaaata ccaaaaaatt
      241 ctcaataaaa cagcttcctc aatcacttca caacgatggt gaccccgttt cccatttaaa
      301 aagagtagcc ttatttctcc cctcccaccg ctctggaatg agcaagttca cccacagtta
      361 gcgaaaaatg aaactatcgt cgcttggctg gaattggatt taaccgatca attatttttt
      421 tcgaaaggca ttttgattgt ttccaatcga cgtttactct caaaaatgca aaatgaggag
      481 cattggctta gctttgttta tcaaaaagag ctagaactaa cgactcacga tcatgcaggc
      541 gtaggtcaca tcgaactgtt tgatacgcaa tcacgactcg cgcattggtg ctatacgctt
      601 ggcaagcttc attttgttca tcatttagtg gaatgtttca accaacaatc aaattttcat
      661 ctagaaggct tcccggaaaa tttggtcact gactctacgt atgatgaaat aggatcaact
      721 gataaaaatt caacatcgat agaagagcaa acctctcgag aaactgtttc tactttcact
      781 ttattaaggt tatggcgatt tgcaaaacca taccgagggc gtttactgat aggttttttg
      841 ctaatgcttg ccagtactgc agcaacactg gtgccacctt atttgacaat gcctttaatg
      901 gatgaagtgc tcattcctta tcaaaatggc tatccactta atgtcgatct agcaacgctt
      961 tatctcggag ggttattggt cgcagccatc ctcgcatggc tgttaggatg gtctaaaact
     1021 tatattctag cgaaagtatc agaacgaatg ggggccgatc ttcgtatcac aacctataat
     1081 catttgctaa aactttccca cgaatatttt ggaaccaagc gtacaggcga tttaatggcc
     1141 cggattggtg cagaaactga tcgtatcaat ttatttcttt ctttgcattt actcgatttt
     1201 gctaccgacg tgatcatgat gatcatgact gcgatgatct tggtttcaat caacccctgg
     1261 ttagcattgg ttacgctact acccctgcct ttcattgcgt ggctaattca tttagtcaga
     1321 gacaaactcc gaacgggttt tgagaaagtc gatcgtgtct gggcagatgt aaataatgtg
     1381 cttgcagata ccataccagg cattcgagtg gtaaaggcat ttgcacaaga agaacgggaa
     1441 gtaaaacgct tttatgatgt taatcagcgc aatttagaaa ttaacgatcg agtaaataag
     1501 gtctggtcct tatttacgcc tactgtaaca ttgctaaccg agattgggct attgatcgtg
     1561 tggatttttg ggatttggca agtatccgag aatcaaatta cggtaggcgt actaacagca
     1621 tttttagctt acattgggcg tttttactta cgcttggatt caatgagtcg aatggtttca
     1681 attacccaaa aagctgcagc aggtactaag cgtatttttg aaatattaga tcacgtgtct
     1741 cgtgtacctg aaccggttaa gcctattcac tccccaacta ttcaaggtca tatcgagtta
     1801 cgacatgtgg gattccgcta tggagtaaga cgtatcactg aagatattaa tctgattatc
     1861 gagccagggg aaatgattgg tttggttggt catagcggat cgggaaaaag tacgctcatc
     1921 aatttgattt gtcgttttta cgatgtcacc gaaggtgtca ttctagtgga tggcatcgat
     1981 attcggtcat ttcccatcgc tgagtatcga aaacatatcg gattagtctt acaagaaccc
     2041 ttcttgtttt ttggtacgat tgcagaaaac atagcctatg gaaaaccaca tgcaacacgt
     2101 caagaaattg ttgctgcagc acgagctgcc catgcgcatg aattcatact caagcaactc
     2161 catggctatg attcccttgt tggagaacga ggacaagcac tttctggagg agaacgtcaa
     2221 cggatttcaa tcgcacgcgc attactcatc aaccctcgta tattgatact tgatgaagca
     2281 acctcatcgg ttgatagcca aacagagcaa gagatacaag ccgcgttaga taatttagta
     2341 cgagggcgaa ctacgatagc gatagcacac cgtttatcga cattacgcca agcaaatcga
     2401 ctgatcgtgc tcgaccaagg aaaaataatt gaggtaggca gccatgccac actaatggct
     2461 aaacgaggct attattacca gctttattta tctcaaacag gccatatcgg tgttgatccg
     2521 tccttgattg attccaatag ggaatcgaac caaacgaaag aatggatatg aattacacaa
     2581 agtttaagct aagccgggat aggcatcgca agctgatact atttgatgat gcgtatcagc
     2641 aaaagagcca tcaggtgatt gcgattcgcg ctttcccgat cagtgcagca gataagggaa
     2701 tcgccttggt tgatcatcaa ggcaacgaat taatgtggat agaaaatctc caagatcttc
     2761 cagtggaatg gcgtgagctg attgaagaag aacttacgag tcgagatttt atgcctgaaa
     2821 ttaaaaaaat cgatagcgta tcaagtttta ttacaccctg tgtttggtgg gtagaaacta
     2881 accgaggtaa tacttgtttc acattaaaag gtgaggaagc gattcgtcga ttatcctcat
     2941 tggctttgct aattactgat caatatggcg tacatttttt aattaaggac cgtacaaaac
     3001 tcgatcacta cagcatcaaa ctccttgatc gctttctctg agaatcaaac taaatatcag
     3061 aattttatgt aaaccgattg ttgcctactg cgttagttaa tggtgtttct cctattccta
     3121 ttcaaagtag cttcatgcat tctaactata ctaaccgtca gtttgatctt aaatttcttg
     3181 aaattattca tttaaacggc cctaaccggt ggacctatta tccggcctta gaagcattgg
     3241 ttgatatcgg tgcacttgag gattatccct ctcatattat tcctggattt tatgatcgct
     3301 tagcagcttg gttgccctct ttaattgagc atcgctgtag ctatgaagaa cggggggggt
     3361 ttttacaacg agtacgtgag ggcacttggc cctgccatat tcttgaacat gtcacattag
     3421 aactacaaaa cctcgctggc atgaaagggg ggtttggacg cgcgcgtgaa accgctgagc
     3481 gcggtgtcta ttgggttgtc gttagtgcgt ggcatgcaga gattaccaaa caagcactat
     3541 ttgcagcacg tgagctgatt cttgccgcaa tgggctttga agcaagacaa gttgattatg
     3601 atgttcaagc tgcgattgct caattacggg atatggtaga ttcattatgg ttgggtcctt
     3661 cgacagcctg cattgttgat gcagcgactg agcgcaatat tcctgcaatt cgtttgcttt
     3721 ccaagggaaa tctcgttcaa ctaggctatg gatcaaatag tcgccatatt tggactgccg
     3781 agacagatcg tacatccgct attgctgaaa atatttcgcg tgataaagaa ctcaccaaaa
     3841 gtttgctgcg tgcttgtggt atccctgttc cagaaggtcg tattactcat agccctgaag
     3901 atgcttggca agcagcagag gcactaggtt tgccagttgt cattaaaccg tgtgatggga
     3961 accatggacg aggggtattt atcgagttaa gtcaatgcga agaaattaaa tcggcctatt
     4021 cggttgcttt aaaggaaggc agtggcgtat tagttgaacg atttgtatca ggatttgagc
     4081 atcgacttct cgtcgtaaat ggtgagctaa tcgcagcgac tcgaggagat atgatttccg
     4141 tagtaggtga tggaatttcc tgcattgctg aattaattga acaacaaatc aattccgact
     4201 ctagacgggg aacaagcgaa gaacatccgc ttaatcttat tcgactcgat agtgccgccc
     4261 aattggaaat tgcgcatcaa ggttattctg cggattcggt tatacctgtc aatgcaagag
     4321 tgctcattca acgaaatggc aatcatgcat ttgatgtaac cgatgaggtt cacccaagca
     4381 ctgccaaaat agccgccctg gcagcacgcg taatcggctt ggatattgcc ggcatcgatc
     4441 ttgttgttga ggatatctca aagcctttat tagaacaatc tggagcgatc gtcgaagtta
     4501 atgccggccc cagtctattg atgcatatta agccagcagt tggtaaatct cgtccggttg
     4561 gtaaagctat cgttgagcac ctttttcctg aaaagggcaa tggtcgtatc ccagttattg
     4621 gaattactgg aagctatggc aaaaccactg ttgcacactt gttaactaag cttatcagtt
     4681 tatcaggaaa acgagttggt ctggcttcga gtaaggggtt gttctttgaa cataaacgag
     4741 ttgatgaaag aaattctgca aattggtcgt cagcaaaccg cgtgctaatg aatcggatga
     4801 ttgatgttgc agttctggaa aatagcttta atagccttct aagacaagga ttagcttacg
     4861 atagttgtca ggtgggaatc attaccaata ttgatcccaa tttgcactac ggtttttacg
     4921 gaattgcaac gccagaacaa gtatatgctg tgatgcgaac tcaagtcgat gtggtaacac
     4981 caactgcagc agcaatcgat gatgtaatca aagagattac tctaccgatg ggttctgcta
     5041 tcttgaatgc gagagactca atgctagtga ccatgtcgca actttgtcat ggcgaagtga
     5101 tttatttcag tgttgatcca gatttgccaa gtatcaaaca gcaccgtatt gagggctcag
     5161 ggagcgcttc cagaaaacga gcagtattag tacgcgatgg cagtatttta ctagtcgatg
     5221 gtacgaacga aatggttttg attcaactca ctgaactttt accgcctacc gacagtgaat
     5281 gtattgtagt ggaaattgag aatgttctcg cagccatcgc tgctgcctgg gcgttagata
     5341 tatcgcctga tgtaatgcgg gtgggaatcg cagcgtttgg atcaagtcag caggaagcgc
     5401 cttgccaaag ccaattttca tgaaataaca aaagcacaag ggtatagtat gaaattaata
     5461 caaagtcgtg tattacgcgg tcctaattta tggagtaaac atccctcaat cgaagcttta
     5521 ttattatttg atgaaggcgc ctgcttgatt gaggacattc ctgaatttga atcacgcctc
     5581 cgtgaccgct ttcctgaaat tgcattacag tatccagcta acagccatga cgttagaacc
     5641 attgcacaag tacttgagtt tgcggtactg ggcttacaga accagattgg gtgcccagtt
     5701 acgtttggac aaaccatttc aatgcccgat aagcgcaact atcgtgtcat tgttgaatat
     5761 agtgaagaag cagtaggtca actcgctttt caatatgctc atgatctttg tatcgctgca
     5821 atcgaaaatc ttccttttaa tttaaatcac gctttgcagc acatgcgcac gctttatgaa
     5881 gatattcgct tgggtcccag tactggggca attgtcaaag cagcgatcgc cagaggaatt
     5941 ccctatcgtc gtttaacgga tggtagcttg gtgcaattcg gttggggcag caaacaaaga
     6001 cgaattcagg gcactgaaag cgatgctacc aatgcaattg ctgaatcaat tgttcaggat
     6061 aaacaattaa ctaaagcgct gttacaagcc gcaggaatcc cagtaccgct gggacggtgt
     6121 gttatgaatg cagatgatgc ttgggtagct gcgtgtgaaa taggcctacc cgttgtgatt
     6181 aaaccgaagg atggtaatca tggtcggggt gtagccgtca atctgatgac ggaacaacaa
     6241 gttagattag ctttcacact tgccacagca gctgaagatg aagtgattgt ggaacaatat
     6301 gttacaggat cagattatcg tttattagtg gtgggagaga aagtaattgc ggcagcaaga
     6361 cgtgatcctc ctcaagtgat tggtaatggg gttcatacta tcagccaatt agttgaacag
     6421 atcaatcgtg atccattacg aagtgaaggt cactccaatt tattgagcaa gatccatctt
     6481 gatcaaatct cattagacta tctcgctact caaggttact cagctgaatc aataccaaat
     6541 aaaggtgtac gtgtcattct gcgaaataat gcaaatttat caacaggtgg cacagcaaca
     6601 gatgtcacgg acgatgttca tcctgaaatt tttgcgactg cgatagctgc tgcaaaaatg
     6661 actggtttag atatatgtgg tattgatatg atttgtgact ccattcgagc ccctttagaa
     6721 gaacagcgag gagcggtcat cgaagtaaat gctgcgccag gtttgcgcat gcacttacaa
     6781 ccctcttttg gaaaaggtcg cgcagtgggt gaatcgatca tcaattatat gtttgctccc
     6841 ggtgaaaatg gacgtattcc cattgtcgca gtcacgggaa ccaacggtaa aacaactacc
     6901 gttaggctca tcaccaacat acttagtatc catgggatgc aggttgggat gacctgcacg
     6961 gatggtattt ttgttgatag tgatggtatt gataccggcg attgcagcgg acccaaaagc
     7021 gcgagaaatg ttttatttca tcctaaggta gatgctgcgg tgttagaaac tgcgcgggga
     7081 gggatgttgc gtgaaggact tggttttgat cattgcgatg tagcggtcgt tacaaatatt
     7141 ggtagaggtg atcatctagg tttagcgcat attaatactt cagaagaagt ggctcttgta
     7201 aaacgcatcg ttattgaaaa tgtattgcct aaaagggggg tcgcaatact caatgcggat
     7261 gatccgctcg tggttaaaat ggctgatcat tccccaggct cagtcatttt ctttactcag
     7321 aatagtcgta atccagttat gttgacccat cgtattcagc agaaacgcgt gatttatctg
     7381 gacaaaaaaa ctatcatcgc atcgagcatt gatactgagc agcgctttgc attgagcgag
     7441 attcctctaa cccaaaatgg tttgattgga ttccaaattc agaatgtcat ggctgcgatt
     7501 ggtgcaggat gggcgctggg catcgattgg gcaacgattc gagcggggct agccaatttt
     7561 gtaagtgatg tgcagacggt cccaggccgc tttaatttct tcaattatcg gaatgcaacc
     7621 ttgattgcag attacggaca taatcctgat gccattgaag cattggttga tgcaattgat
     7681 aatctacctg caaagaaacg aatcgttgtg attagcgcag ctggtgatcg tcgtaatgaa
     7741 gatatccggt tacaaaccca aattttgggt gatgttttcg atgagatcgt gctatatcaa
     7801 gatcaatgcc agcggggacg ttccgatgga gaagttctag gactgctgcg agaaggatta
     7861 gagcatgcta agcgtgttag caaagtgagg gaaatccacg gtgaatttaa agcaatcgat
     7921 acggcattaa ccaatcttca agccggcgaa ttgtgcttga ttctggttga tcaagttgaa
     7981 caatccttaa atcatattcg cgaacgggta acgtcacatc gctgactgag agccaatttc
     8041 atcgattatt taatgactca gatggtgagt cgggtgcggt tcagcatcag aaggtatatc
     8101 ggttgtccag atttgatacg ccgagtgaca tgcaacacat tgctgcaaag tcatacttaa
     8161 ctgagacaat gtatgcctag aatcttttag cgtctctgcg tctgtggcga tcttatcaaa
     8221 gcttctatga actgacatac cgagttgcat aaaatcatcg ggcagaacag attgcaaatg
     8281 atcttcacct ttatgcgcca tttccatacc cagcatacgc gcatatctcg ctactgctaa
     8341 catatcatct tctgcaagtg cttgtatgat ttgctgtgtc cctaaaagca tagaccgcat
     8401 ctcggataaa atatgatggc gctgcgcttc attcagtaat agtgcttttc ggttatcagt
     8461 ttcattggct gcatttaacg gaagcacaaa aaagcatatt agcgtgctga taataatttt
     8521 tttcataaaa aacaccctct cgtttttttg ttaatcttta cagcactcaa tgtggcgcca
     8581 accacaatgt ttgctgcgct ataccaggaa gaaatggggt tggtttccca cttacccaat
     8641 cctgatgacc gcttccatag taaggtgaaa gtggatggcc actttgccca ccaggcatag
     8701 aaaaataacc ttgctcttca ttaccaggcg caacaacaaa tcgttcagaa gccccgaaac
     8761 tcggtgcttg gatacgaggc ataaaactat cacctggcag taaatcttta ggcatattta
     8821 accaccaagc aacaaaattt ggtaaaactt tacttaacgg atgttgtatc tgagcagtat
     8881 tctgcccccc ccaattccaa ggtttatcat cgccggattg cgtttccatc agtaaggcca
     8941 cttttcttgc acatttttgt aaaaacgctt cccagtttgc attgccgaga ggaagcaaat
     9001 gaggtgggcg attctctatc aaccgccata ttgcgtgttc aatttgcggt agtcgcggag
     9061 gcgaaaaatc tggatactga tgctttacga ccgcaataaa attacgataa atgctatcaa
     9121 ctacttcctg cctgaaagta cgaacgattc ggtaggaaac tgagcttggt actgcaagac
     9181 catcccaatc cagcaatcca tcttctaatt tctgcttcca tggttcaatc ggtgcatgat
     9241 ctatcacttc atgaagaagc ttctgccagc gagacaaaaa taatgcgcga tgatctaatt
     9301 gcacctcgaa caaatctagt gcagtaaatt tttctttggc aaacaaattg tcacgaatct
     9361 gttttgaacg cgcacccaat tcgtatccac catctccaag ttttactaat gcaagaccat
     9421 cgatcattct ggcattagca ctccataagc gcatattcat tggattacta atcaaagggt
     9481 aatgcgccga aggtaaccat cctaaccaac ctgtttcacc atttggccaa tctgaaggaa
     9541 aattggggtc ataattacca gatctcatcg gtatacgccc agcaatggtc catccaatgc
     9601 ttccttcgcg atcacccaca ataaagtttt gggctggtat tccgctctgt tgcgcaatct
     9661 ctagtgcctg atgagcagtt tctacccact ccaagcgcat caatttaaga tcagcagcat
     9721 cacgatgctg agcaacccac ttcagcgcaa gtgctgtacc gtcatgatca tgacctagaa
     9781 tgggtcccca ctccgtctct tcaacttcaa gaaactcatc ttcagcatcg cggacatgca
     9841 gaatttcttg aaaggttgcc aatgatttcc aatctccgtc ggaaagatat cgtgttgaat
     9901 gctcgggatg acgaacaaca cggacccaat ccgctacatc gatatagcta ttggtgaaac
     9961 tccaggcaac attaccattt gacccaacta cgattgctgg cacaccgggt agcgtaacac
    10021 cactaaccgc tacattgatt cctgctttat tgggatgggg ataaattaag cgcgtcctaa
    10081 accatatatt aggtactcgt agggtaagat gcatatcatt agcaattagc gccgcaccat
    10141 cggttaaact acccgataca gcaaaactat tactacccgg tgcttcatca tggagttcta
    10201 accgatcctg ttccattgtt ggcaaatgtg catatgcctg aaaattaaat tcctccttgc
    10261 ttggtaattg tggccatttt aggggtatcc cgatgagagg tgcatcccaa tccccgccag
    10321 atgcggttaa aaactgatag actgaggcag gtaaaaccgt ttgtaaagct gatagctgta
    10381 gctctcggtt aagacttgat tcttgcagcg taaaatacat cgccaatata actagcaaag
    10441 tatcttcatt ctgccaagga actggacgtt ctcgtatcaa taaataggga aaagactgaa
    10501 tgagtaatgt agctagtcct gcattaactc ctttacggta tgactcaatt agagcacgtt
    10561 gatcctctgg taagttatca aacatatgct tggctcgagt tctcatacgg tgttttcgta
    10621 tttgtctatc ctttggtaaa gctgctttac caaataattc cgctaactca ccggccgcac
    10681 ttcgacgcat caaatccatt tcaaagaaac gctcttgtgc atgcacataa ccaagtgcct
    10741 gcgttaaatc aagtcgattc atcgcttgaa tcgttgccgt acccaaagcg tcacgatcaa
    10801 ctctaactgc ctgagtcaag cttgaaagct tagcagaacc tgagtattgc ggtaagctag
    10861 cgctcaacac aaaccaaaag atacccccta atccaagcag aaaaaaagcc aagccgagaa
    10921 aaagcttgtt tttatttata atagaatgag tcatcagtga tctctcattt taatgagatt
    10981 tcctccaact gtttacggat ccactcaatc cgttgtcgat taacaccaag atcactcttg
    11041 cctaatcgag aggcagatcg tacttgcacg acatttgact gaggatcaaa ccaaaactct
    11101 gcatcatcga caaacttcat cacgcgcgtt gtaaattgca cataaagata gttttcttga
    11161 ttggtaatta tttgagagcc aggcatcagc tcgataatcg ctttaatttt actcaaggta
    11221 gatttgctat cgcctaccaa tggtaatggc tcaattcggg catagttatg ctgcgggtga
    11281 ttggggtaga gatctgcttg gcttgatacg ctatttggcg tatccgcagg caatttcagc
    11341 ctaccttcga caacacccag gtcgttgggt gcgcccccct taaacatccc gagctggcct
    11401 gctagaattg ctacaataac aagaatcagt atcaaaacca agaaccactt taacgctatc
    11461 atgttaggaa aatcctatta atttaaaatt aaaaatacaa ccaagaaacg ttatgccaga
    11521 aagtagaaat aatctatcaa cacaattgat acaagatcct aacgaattac ctttaatctt
    11581 tcattcccgc gtcaatgaac ccatctcaaa tctatcatta cttcgcaaat cattgttatt
    11641 tgctgaatta tcgatgatcg cttataacga ttctaatgaa gcaaaacgtg ctgtcaattt
    11701 gattggtttc ccagtgagta ttttttttga ttatgatggc gctcaaggtt ttttcttcga
    11761 aaatgaccac gatgccgtga tcgtttgccg aggaacagag cccaatgagt ggaacgatat
    11821 ccatgcagat gctaacttag gaaccgtatt agcagaaaca gcgggacgcg ttcaccgagg
    11881 ttttaaacaa gaagcagatg atctctggcc catgatggaa aagcatctaa tcgatttagg
    11941 caaaacgttg tggtttactg gccactcatt aggcgcagct atggcaaccg tgtgcgccta
    12001 tcgatgccat gtttcaaaca aatgtgctag cccaaccgaa ctctatacat ttggtagccc
    12061 tcgcgtcggg aataaacggt atgtcaactt tgtcaagcta aagcattatc gttgggtcaa
    12121 taataatgat attgtgacaa gagtcccccc gatatggctg ggttatcgtc atatcggcat
    12181 tgaaatgtac ctaaaccgta acggtctttt acgaacactc gattacgcaa ccaaacgatt
    12241 agatcgctgg cacggattcc tacgcggcat tcgcaaattc cggatagacc acttttctga
    12301 ccatagcatc catgagtatg ttaaccatat tttaaaagcc gtacaaaaag atgaaaacaa
    12361 cgcagcgagt taacaaatct cgccattacc aatcactgac taatcgttaa cattgcaaat
    12421 tatcgatcag cccaaatcaa tattgcatta gttatataaa aaactactgc tattttcatc
    12481 tcagttaaga aaaataaaca gttatgatat gaagtaattt ttattcgttt ggcttattta
    12541 gctatctaaa cataatgcgt atcctgcata tacttgatca ttcgattcca ttacacagcg
    12601 gttatacatt ccgaacacgc tctattctga aagcgcaacg cgcgttagga tgggaaacgt
    12661 ttcatttaac cagcactaag caaggtttga caaactcgct tgaagaagta atcgataatt
    12721 tccactttta tcgaacaccc gttaataatc gttttatcaa cacattgcct atcattaatc
    12781 agatagcagt aatgacaagc cttactcaac gattaaatga agtagtagat atcgtcaaac
    12841 ccgatatatt gcatgctcac tccccggtat taaatgcgat accagcctta tatgttggca
    12901 ataagaaagg tattccagtt gtttatgagg tcagagcatt ctgggaagat gcagcagttg
    12961 atcatgggac gagtaacgca gggggcctac gttatcgttt gacacatggc ttagaaacct
    13021 atgcattaaa gcgtgctaac gccattacca cgatatgtga aggattgcgt aacgatattg
    13081 tagaacgagg aattgaagcg cataaagtta caacaattcc caatgcggtc gatctcgcac
    13141 agttttcaat gaatcattcg attgatcaat cactgaagca gcgtttgggt cttcaggata
    13201 agcgcgtcat cgggtttatc ggttcctttt atgcgtacga aggtttggag atcttattgc
    13261 gatcgttacc aaacctatta cctatatatc ccaatctcca actattacta gtgggcggcg
    13321 gacctcaaga aaatttctta aaacaaacgg ctcaaacact tggaatacaa gacaaagtta
    13381 tcttcacagg ccgagtaccc catgagcaag tagaaaaata ttatgatcta atcgaagtat
    13441 tagtttatcc gcgattatcg atgcgattaa ccgacttagt tactccgctg aaaccacttg
    13501 aagcaatggc gcaaggtaaa ttatttattg cttccgacgt aggaggtcac cgcgaattaa
    13561 ttcgcgatgg cgaaacaggt cttttattta aatccggcga tccagacgcg cttgcaaaaa
    13621 aaatcatcta tttatttaat acacctgatc tttggaaaag cttgattcaa gctggacgtg
    13681 cttacgtaga gaatgaaaga aattggcaaa atagtgttgc acgttataag accgtttata
    13741 accagctcct cgtcgaacaa ggtaaaaccg ttgaatcatt ataaaatgca tatcggactg
    13801 gttggtccat taccaccacc atccggagga atggcaaatc aaacgaggca gttagctcat
    13861 ttgctaacgg atgaaaatat tcaagttacc ttaattcaag tcaatgcccc ctattacccc
    13921 cagtggattg aaaaattcaa agggatccgc gctttctttc gactaattcc ttacttgtac
    13981 aagctatggc aagcctctca ttctgttcaa atttttcatg tgatggcgaa ttcaggttgg
    14041 tcatggcatc tgttttcagc acctgtaatt tggatcgcat acctgagaaa taagcctgtc
    14101 atcatcaatt accggggtgg agaagctgaa gcctttctta ataaatctat tttttgggtc
    14161 aggcccagct taaaaagagt aagcgccatc atcgttcctt ccgcatttct taagcatgtt
    14221 ttcaatcagc aaggatttga tgtaacgatc gtacccaata tcatcgatct tgatcgcttt
    14281 gctgcacaga ctcaagaatg gaacgtacct aaaattgaga atagtcccca tatcatttta
    14341 acgcgcaacc ttgaatcaat ttatgataat gcgacagcaa ttcgcgcatt ttctatcgtt
    14401 aaaaagaatt ttccgaatgc gaagctatcg atagcaggct caggccctga gaaactgatg
    14461 ctagagcagc ttgtagatga ccttaattta gtagactccg taactttcct cggtcgagtg
    14521 gataatactg ctatcaatga tttgtatcgt caagcggatg ttatgatcaa tacgagttta
    14581 attgataaca tgccaatctc gatattggag gcactcgctt gtaaagtacc agtagtcagc
    14641 accaatgttg gcggcattcc attcttagtc aaacatgatg aaactgcatt actggttcca
    14701 ccgaaaaatc ccggagaaat agcagaagcc atccagcaca ttctcactaa cccagaaaaa
    14761 tccagaaaaa tggctgaggc tggatatgaa atggtacaac actatgcgtg gaagaatgtg
    14821 cgaactcaat taatggaaat atatgagcgt actctctctc atcggttcta atttaataac
    14881 gaaaccgtaa attatatgag caccaatcct gtcaataagc atttatatac ttggtttatt
    14941 gcaaatattt gttttccgat tcatgaattt ttcaaacgcc atcatacagt ttcgattagg
    15001 agagaacttg aaatcacgca atggtggaat aaaagccaaa tagaacagct tcagctctca
    15061 aaactaaaac aactgctcat ccatgcaaaa gcaaatgttc cttactatca aaaaataatg
    15121 cgtgacctag cgattgaacc tgagcaattt gaatcgcttg cagcgttatc taagcttcca
    15181 cttttaaata aagcagcgat tag
//
LOCUS       DAOQLJ010000044        15052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1443_length_15052_cov_5.439288, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000044 DAOQLJ010000000
VERSION     DAOQLJ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 15052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15052
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1443_length_15052_cov_5.439288"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PK522_04760"
     CDS             complement(<1..1144)
                     /locus_tag="PK522_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41076.1"
                     /translation="MKLRLRTSSGKYLSCDGASDTTPLNAGKSNKGETEEFSLVINNQ
                     VISSDHRGTLRISSGDRGTLRTTRNLTTFHMSQNYRQDAYVTTIDSRGRHQNRLFVQT
                     HFFGEKGDDFIITRLNRDGEVLLDGDEIALKFVETDKPGFLSSDSSGLLRIINKDNPG
                     SQEIFVVETMSELKLFWSDQRADNFSTATPLGELQAIQAGYAFVRTQGLVYMNSMPGC
                     NPLFLFWNNQNGDNLITATKEGEKEANDNGYVKVRIEGWIKSKPEPGTIPLRMYWKGA
                     ERKDSFTTTSIKEEQDAANAGYEFVRDEGYIIPPLSVIIPSVSILTPGTIQPNFDSIE
                     GVGNQNIDNSDINREGLINININSPRRRRGANKKALILSGGGAKGCF"
     gene            1404..1811
                     /locus_tag="PK522_04765"
     CDS             1404..1811
                     /locus_tag="PK522_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41077.1"
                     /translation="MNGKSPDWISLIETSYSLEGNDSQWLRGLIDHVSKILNRGSEPV
                     GWTFHCTPTKFKLGSFSEGTSKALTYAARMSHALSTEKSLDLTYRTGVVIATASELVF
                     PRLPDMHKMFLNLLCCSKKLHKLRYRNVGVGLR"
     gene            1751..2599
                     /locus_tag="PK522_04770"
     CDS             1751..2599
                     /locus_tag="PK522_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNC41078.1"
                     /translation="MLLKKTSQVTVQERRRWPQVAAHIGAAVRLRGKIAQLSSIDSPE
                     VEAILDSGGRLHHAQESAKDQDIREKLRAVVRRIEQSRTQAGRQNADAALNNWEGLVN
                     GRWSIVDRFDSDGRRYIVAIKNDPAHPDLRGLTPRERQVAEYVGLGYASKEIAYTLGL
                     SETAITNCTARTQSKLGIHSRAELVNFFAPNGFRRKLAETVLNGEHLLIATNPLIKSH
                     QLIGLTETEREIATYLIAGSTNADIARRRGSSEYTIANQVQSIFRKLSVRSRGELIAK
                     LQNEKI"
     gene            2834..3799
                     /locus_tag="PK522_04775"
     CDS             2834..3799
                     /locus_tag="PK522_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNC41079.1"
                     /translation="MALFRFSTQGFSESERVNAFQEIYASVANIDVEPYQQHPPFVEM
                     VGRLLPNVGVYETIISPHSSWRTRAHVAAENSDNLALVVPIDNTAIVIPKNKEPLHCL
                     PGDAVLIPSDSIHQALNTQTMSIAVIVLPTKLVATRNADYNERLMKKISSKDTPELPL
                     LVGYTRMLMQMKDDLSPELAALAATQIHDLFVLLCGAKQEEAAIAKDRGLRAARLRAV
                     KQDIVTHITDCELSINQVALRQGISPQYIRALFHSEETTFADYVTGLRLDQVYRFLRN
                     PLHLDRSISTLALDMGFNNISWFNRIFKQKFGLTPSEVRNSLINR"
     gene            4038..4769
                     /locus_tag="PK522_04780"
     CDS             4038..4769
                     /locus_tag="PK522_04780"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNC41080.1"
                     /translation="MATQAELDAAVAAQATVDAGQNTTINTQQSTIETLQNEITSLNN
                     VNTAQQSALEKAQADIADLKVLIEKYHPEPKKIGDHFGGGIVFYIDPTGQHGLITSLF
                     DLSDKYIPWFNGINKITGATGDGIGVGAPNTALIVAAQTGDGGPDFYAAKLAADFSVQ
                     EDGVKPCTGEVGETCYRDWYLPSKFELNLLYQQKTVVGNFMPNEYWSSTEYDANNAWL
                     LNFTDGTQYFTDKYFTVRVRAVRAF"
     gene            4808..5407
                     /locus_tag="PK522_04785"
     CDS             4808..5407
                     /locus_tag="PK522_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41081.1"
                     /translation="MKLSKFFALSFFISSFLLIVLSLQVSAAPNQPAMNVTGSVDANV
                     TNNVKVINPDGSALEVRIQPKKGSYTLSLNATATNDRGSFSTVIIPFDSYISGINFTT
                     GSKDSASCSTFVSYQGGGIPEGANTNEIAETGFASDGSRYAAPLYIMNSEYISIPNMF
                     IRGGDELLLTVTMNTHVFGATGNESCLGRAKIYLIPADK"
     gene            complement(5624..6787)
                     /locus_tag="PK522_04790"
     CDS             complement(5624..6787)
                     /locus_tag="PK522_04790"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HNC41082.1"
                     /translation="MDPETLALHTGIHRSQFNEHSEAMYLTSSYVFESAAQAAARFSG
                     EEPGNIYSRFTNPTVTAFQERLAVLEGAEECIATSTGMSAILACVLGLLSAGDHIVAS
                     RSIFGATVNLFNNILSRFNITTTFVSATETQEWADACKANTKLFFLETPSNPLTEISD
                     IAAIAQVAKNAGARLVVDNCFCTPILQKPLELGADIVIHSATKYLDGQGRVLGGAILG
                     KRDLLVDGGIYNFLRTAGPTLSAFNAWVLLKGLETLKIRIEAHSAHALEIANWLTEQP
                     NVARVFYPGLPSHPQHHLCKLQQKTGGGIVSFEVKGGREAAWRVIDNTSMISITANLG
                     DAKSTLTHPATTTHGRISQEARSAAGITEGLLRIAVGLESPKDIKADLAKGLG"
     gene            complement(6856..8376)
                     /gene="purF"
                     /locus_tag="PK522_04795"
     CDS             complement(6856..8376)
                     /gene="purF"
                     /locus_tag="PK522_04795"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNC41083.1"
                     /translation="MCGIIGVVAQSPANQILYDGLLMLQHRGQDAAGIVTAQDNTFYM
                     HKGLGMVRDVFRTRNMRALLGNMGIGHARYPTAGSADSPAEAQPFYVNSPFGIVLGHN
                     GNLTNAEELNKALYRSDRRHVNTNSDSEVLLNVLAHELQACARNDQLDPDDIFAAVAG
                     VHERCQGAYAVIAMIAGYGLLAFRDRLGIRPLVFGTAETEQGIEYMVASESVALDTLG
                     FKLIRDVAPGEAIFIDTKGNFHNKQCAANSSLNPCIFEYVYLARPDSMMDGFSVYETR
                     LNMGSSLAEKIKKTMRHLDIDVVIPIPESSRPSALQLANHLGIDFREGFVKNRYIGRT
                     FIMPGQQNRRKSVRQKLNAINVEFQGKNVLLVDDSIVRGTTSREIVQMAQEAGAHKVY
                     FASAAPPVRFPNVYGIDMPTRQELIAADKDEEQICYEIGADFLVYQELESLKNSISRI
                     NPAIKNFETSCFDGHYITEGITQDYLDKLEFQRSAGAKKLTKHDLTQLDLGLILAD"
     gene            complement(8434..8928)
                     /locus_tag="PK522_04800"
     CDS             complement(8434..8928)
                     /locus_tag="PK522_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNC41084.1"
                     /translation="MTILDYIVIGIGFISVFLGITRGVIKEVISLAGWFIAFYVASRY
                     AESFEPLLPRVIEDPSLRMLTLFVVVFFIVLLLVMFCSILLSKLIRSVGLGLIDRVLG
                     SIFGLARGFAIVLSLVLAAGFTALPKQPFWQQAVLSDPLEKAAVQVIPWLPESFRGHI
                     SFDK"
     gene            complement(8972..9622)
                     /locus_tag="PK522_04805"
     CDS             complement(8972..9622)
                     /locus_tag="PK522_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNC41085.1"
                     /translation="MVRNISEEEELLRKRARRRLLGAITLVTLLVIFLPMLFDEESTQ
                     ERQEIDIQIPSEDLVTENYPWMMPTDPSSDETDASTEQPDELLAMIPQGSPQTAEDAQ
                     EESSNDQGTPIPLSKPQYHKPVTQLNKTPETPQPKAAEGVFVIQLGAFSDPLKAKQQL
                     QNLTASGINGAYVETIKVGHNEMVRVRVGPFSTRKAAEDEYEKLKRMGLSGVVTSR"
     gene            complement(9663..10931)
                     /gene="folC"
                     /locus_tag="PK522_04810"
     CDS             complement(9663..10931)
                     /gene="folC"
                     /locus_tag="PK522_04810"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HNC41086.1"
                     /translation="MESRSLADWLAYLENLHPFTIDLGLDRVKRVKDNLCLTPSFPVI
                     TVGGTNGKGSICAMLETTLSAAGYRVGCYTSPHLLTFNERIRILQQEVSDTEICRAFE
                     TIESARLQSQVTLTYFEFGTLAAMHLFIESNVDVTILEVGLGGRLDAVNIFDADCAIL
                     ASIDLDHMDYLGETREEIGLEKIGIFRKDRYAICSEPDIPPSVQMKIDELGAKFFRIN
                     QDFSYSTQDNLQWNFYGIHNNVYALPYPSLRGSFQLRNASACLAALDCLKDELPVDNQ
                     AIRRGLVETTLKGRFQVLPGSPRVILDVAHNPAAAKVLAENLDQMGPSFQKTYAVIGM
                     LKDKDIASVIQILKNHIDVWLIGTIHSARGAQAEEIASIISNITHSNAIHSFSDLSDA
                     YAFAQKQAGKNDRICVFGSFYTVSAILAFT"
     gene            complement(10994..11887)
                     /gene="accD"
                     /locus_tag="PK522_04815"
     CDS             complement(10994..11887)
                     /gene="accD"
                     /locus_tag="PK522_04815"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNC41087.1"
                     /translation="MSWFQRIIPPKIKPKENGEKKVVPEGLWSKCIACEAVLYYTDLA
                     KNFNVCPKCGYHNRISARNRIDMLLDPEERIEIGVEVKPQDPLKFRDSKRYTDRLIQA
                     NSSTDEDDALVVMQGKIKTTPVVVAAFEFGFMGGSMGSVVGERFVRGVRVSINQHLPF
                     VCFAASGGARMQEGLFSLMQMAKTSAALTQMSKEKLPFISVLTDPTMGGVSASFAFIG
                     DIVIAEPGALIGFAGPRVIEQTVRQTLPEGFQRAEFLLEHGAIDLIVDRRHMREKLVN
                     LFTLLMRTPSPKSAATLLPPS"
     gene            complement(11921..12724)
                     /gene="trpA"
                     /locus_tag="PK522_04820"
     CDS             complement(11921..12724)
                     /gene="trpA"
                     /locus_tag="PK522_04820"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNC41088.1"
                     /translation="MNRIAATFSKLKSANQKVLIPFITAGDPKPEVTVPLMHTLVKAG
                     ANLIELGVPFSDPMADGPTIQRSSERALANGVSLANVIAMVAEFRKTDTLTPVVLMGY
                     ANPVEAMGVDHFISTAKESGVDGILVVDYPPEESTEWAQQLSAAGIDPIFLLSPTTPI
                     ERVKQVAKLAQGYLYYVSLKGVTGASHLDLTEVQTKLAQVRTLVSIPIGVGFGIRDAQ
                     TAKAISKLADAVVIGSRIIEEIEKAPENSLLETLSNLVSSMRSAIDETN"
     gene            complement(12768..13967)
                     /gene="trpB"
                     /locus_tag="PK522_04825"
     CDS             complement(12768..13967)
                     /gene="trpB"
                     /locus_tag="PK522_04825"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNC41089.1"
                     /translation="MSAYNLPDEKGHFGPYGGKFVAETLMTALEELRTQYLYYQADKA
                     FQTEFAYELKHYVGRPTPIYHAKRWSDHLGGAQILLKREDLNHTGAHKINNTVGQALL
                     AKRMNKKRVIAETGAGQHGVATATVAARQGMECVVYMGSEDVKRQAMNVYRMKLLGAQ
                     VIPVESGSRTLKDALNEAMRDWVTNVESTYYIIGTVAGPHPYPMMVRDFQAIIGKEVK
                     DQMQQEYGRQPDALIACVGGGSNALGLFYPYIDDETVRMIGVEAGGKGINSNQHAATL
                     VTGRPGVLHGNRTYLIQDDHGQIIETHSISAGLDYPGVGPEHAWLKDSNRAEYVAITD
                     DEALSAFHALCRFEGIIPALESSHALAYAAKLSPTLGKDKLLLVNLSGRGDKDMMTVA
                     QYSGITF"
     gene            complement(13954..14580)
                     /locus_tag="PK522_04830"
     CDS             complement(13954..14580)
                     /locus_tag="PK522_04830"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNC41090.1"
                     /translation="MRTRVKICGITRTEDALAAISSGADAIGFVFWQQSARNILPQQA
                     RPITKTIPAFVTTVGVYVDPTVEWVHETATIANLGLLQFHGNESPEFCDQFSLPYVKA
                     LRIKEDMDLLEYANRYQSAKALLLDTYSANLPGGTGEVFDWTLIPANLPLPIILSGGL
                     TPDNVVHAIAKVKPWAVDVSSGVEASKGIKDINKISAFMQRVKNCECI"
     gene            complement(14627..>15052)
                     /locus_tag="PK522_04835"
     CDS             complement(14627..>15052)
                     /locus_tag="PK522_04835"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HNC41091.1"
                     /translation="GWFHEPLNVDDMQKAADSLLGEHDFSAFRTAECQAKNPVRKLTK
                     LKITQQGDFLKFEFRANGFLHHMVRNIVGSLIYVGANRHQPSWITAVLESRDRKRAAP
                     TFSPYGLYLAEVKYDTRWNLPTLNDSLCDITALEVFIKK"
BASE COUNT         4285 a         3254 c         3141 g         4372 t
ORIGIN      
        1 caaaacaacc tttggcgcca ccaccactta gaatcaatgc ttttttgttg gcaccgcgtc
       61 tacggcgagg cgagttgata ttaatattaa taagtccttc tcgattgata tcgctattat
      121 caatattctg attgccaact ccctctattg aatcaaagtt aggctgtatc gtaccaggtg
      181 ttagaataga tacagaaggg ataattaccg agaggggtgg aataatataa ccttcatccc
      241 gaacaaattc ataacctgca tttgccgcat cttgttcttc tttaatacta gtagttgtaa
      301 atgagtcttt ccgttcagca cctttccaat acatacgaag cggaatagtt cccggttctg
      361 gctttgactt aatccaacct tcaatcctta ccttaacata accattatca ttcgcctcct
      421 tttctccctc tttcgttgct gtaataaggt tatctccatt ttgattattc cagaaaagaa
      481 atagtgggtt acaacctggc atagaattca tataaactaa gccttgtgtg cgaacaaatg
      541 catatcctgc ttgtattgcc tgtaactcac caagtggtgt tgctgtggaa aaattatcag
      601 ctcgttgatc gctccaaaat agcttcagct cactcattgt ttcaactaca aaaatttcct
      661 gtgaccctgg attatcttta ttgataattc tcaacagccc agaagaatct gacgataaaa
      721 aaccaggttt gtcagtttca acaaacttta aagcaatctc atcaccatcc aaaagtacct
      781 caccatcacg atttaatcta gtaatgatga aatcatcccc tttttcacca aaaaaatgag
      841 tttgtacaaa gagtctattt tggtgacgcc ccctcgaatc aatagtagtg acataagcat
      901 cttggcggta attttgactc atatggaatg tagttaaatt acgagtggtg cgaagtgttc
      961 ctctatcacc actcgaaata cgaagtgtac ctctatgatc acttgaaata acttggttat
     1021 ttatgactag cgaaaactct tctgtttccc ctttattact ctttccagca ttcaggggag
     1081 ttgtatcaga tgcaccatca cagcttaaat attttccact tgaggttctc aaacgaagtt
     1141 tcatattttt aatctcccac aatgagcagt ctttaaatag ccactgataa tattcggaat
     1201 ttagctataa aacccagatt taaatcaaat cagaataagg ttttttaaca atgctacctg
     1261 atcaggtatc tggttcttca gctattacaa tttatactta taaaataatc taatcccagg
     1321 aataattctc ctactgagcg ctactcaaat tggtagtaaa cgctactctt gatcatttat
     1381 aatttaatag gggtatgttt taaatgaacg gtaagtctcc tgattggata tcattgatcg
     1441 aaacgagcta tagcctggag ggtaatgact cgcaatggtt gagaggatta attgaccatg
     1501 tatcgaaaat attaaaccgt ggatcggaac cggtgggttg gacatttcat tgcacaccga
     1561 ccaaattcaa gctaggcagt ttttcggaag gaacctctaa agcattaact tacgctgcgc
     1621 gtatgtcgca tgcgttatcg actgagaaat ccttggatct aacctatcgc accggtgtcg
     1681 tcattgcgac cgcaagtgaa ttggtttttc cacgcctacc ggacatgcac aaaatgttcc
     1741 tgaatctgtt atgttgctca aaaaaacttc acaagttacg gtacaggaac gtcggcgttg
     1801 gcctcaggta gcagcacata tcggagcagc agtacggttg cgaggtaaaa ttgcccaatt
     1861 gtcttctatc gattctccag aagtggaagc catactggat tcaggaggaa ggctgcatca
     1921 cgcacaggaa tccgcaaaag atcaagatat tcgtgagaaa cttcgtgccg tcgttcgccg
     1981 gattgaacaa tcgcgtacgc aagccggacg tcaaaatgca gatgcggcgc tgaataattg
     2041 ggaaggactg gtcaatggac gctggtctat cgtagatcgt ttcgatagcg atgggcgccg
     2101 ctatattgtg gccatcaaga atgatcctgc gcatcctgat ttgcggggct taacgccaag
     2161 agaacggcaa gtcgccgaat acgtcggcct tggatacgca tcaaaagaaa tcgcatatac
     2221 gttaggtctt tctgaaaccg ccattaccaa ttgcaccgca cgtacgcaaa gtaaactggg
     2281 aattcattcc cgtgctgaat tagtgaactt cttcgcaccg aatggattcc ggcgcaagct
     2341 ggcagaaact gtgctaaatg gagagcattt gctaattgct accaatccgc tcattaaatc
     2401 tcaccagctt attgggctta ccgagacaga gcgagaaatt gccacttacc tgattgctgg
     2461 atcaaccaac gcagatatcg cacggcgtcg cggttcgagt gaatatacca ttgccaacca
     2521 agtgcaatcg atcttccgta aattatccgt gcgttcgcgc ggggaattga ttgcaaagct
     2581 tcaaaacgaa aaaatctaac cgcacaattc aatatcagcc gcatcgtcaa tattactatg
     2641 agcaattaac cataattgta agatcaactt gtatcaaaag taatgtgtgg aattaactat
     2701 tgatgagaat ttaccataat tgtaggattg gcttgtattg aagtatacag ttatttttat
     2761 ataagcgatg tttctacatg gaaaatttca tgtgaaatac agagttataa ataaatctgg
     2821 taaatctgaa aaaatggcgc tatttagatt ttctactcag ggtttttccg aaagcgagcg
     2881 tgttaacgca ttccaagaaa tttatgcatc tgttgccaat atagatgttg aaccatacca
     2941 acaacacccg ccttttgttg agatggttgg taggttgctt ccgaatgtgg gtgtatacga
     3001 aaccatcatc tcaccccatt catcatggcg tacacgcgct catgtcgctg ctgaaaacag
     3061 tgacaatctg gcattggttg tacccatcga taacacggca attgttattc cgaaaaacaa
     3121 agagccgctt cactgtttgc ctggtgatgc tgtactgata ccttcggatt caattcatca
     3181 ggctcttaac acccaaacga tgtccattgc tgtaatcgtt ttaccgacaa agttggttgc
     3241 aaccagaaat gctgactata acgagcgttt gatgaaaaaa atctctagca aggatacgcc
     3301 ggaattaccc ttgcttgtgg gttacaccag aatgctgatg caaatgaaag atgacttgtc
     3361 gcccgaactt gcggcattgg cggctactca gattcatgat ttatttgtgc tgctgtgcgg
     3421 cgcaaaacag gaagaggcgg caattgccaa ggatcgtggt ttgcgcgcag cacggcttag
     3481 agcggtaaag caagatatcg tgacgcatat taccgattgt gagctgtcca tcaatcaagt
     3541 tgctctgcgt caaggcatat cccctcaata tattcgcgct ttgtttcaca gcgaggaaac
     3601 cacattcgca gactatgtta ccggattacg tctcgatcag gtttatcgtt ttctgcgcaa
     3661 tcccctacac cttgatagaa gcatcagcac attagctttg gatatggggt tcaataatat
     3721 ctcctggttc aatcgtatct tcaagcaaaa atttggactg actccctcgg aagtgcgcaa
     3781 ttctttaatt aaccgctgaa tcatctcaaa agtagcgttt agtacaagta gtatagcgct
     3841 cagtagtagc tcaatttgag aaattaacgt aaaacgattg cacggtcagt agagcaggat
     3901 cgtttcttaa ataccataaa aataaggagt aataatgaat aggtatcgga atatcaaatt
     3961 ttcaacaatt atgactgctg ctctgctagt catggctgtt ggattaactg gcaaatactt
     4021 ttgctgccaa aaccgatgtg gccacgcaag ccgagttgga tgccgcagtt gctgcgcagg
     4081 caacggtgga tgctggacag aatacgacaa ttaacaccca gcaaagtacc atcgaaacac
     4141 tgcaaaatga aatcacttca ctgaataatg tcaatacagc acaacaatca gcactggaaa
     4201 aagcacaagc agatatcgcc gatctgaagg tattaattga aaaataccat cctgagccca
     4261 agaagatagg ggatcatttt ggtggcggta tcgtcttcta tatcgaccca acaggacaac
     4321 atggattgat tacttccttg tttgatttgt cggataaata tatcccatgg ttcaatggca
     4381 ttaataaaat cacgggggct accggagatg gtatcggtgt aggtgcgccg aatactgcat
     4441 taatcgtcgc agcccaaacc ggtgatggtg ggcctgactt ttatgcagca aaattggccg
     4501 cagattttag cgttcaggag gatggggtga agccctgcac tggcgaagtg ggtgaaactt
     4561 gttacaggga ctggtatcta ccttccaaat ttgaattgaa cctgctctac caacagaaaa
     4621 ccgttgtagg caattttatg cctaacgaat attggagttc cacggagtat gacgctaaca
     4681 acgcgtggct cctgaacttc accgatggaa cccagtactt taccgataaa tacttcacag
     4741 tcagagtgcg ggctgttcgg gctttttaat gaataatcct gtcttaaact aaaaacacga
     4801 ggttaatatg aaattatcta aattttttgc attgtctttt ttcatatcaa gctttttgct
     4861 gatagtcctc tccttacaag tttctgcagc tccaaatcaa cctgcaatga acgtaaccgg
     4921 aagtgtagat gctaatgtta cgaataatgt gaaagttatc aatcctgacg gtagtgcact
     4981 ggaggtccgc attcaaccca agaaaggttc gtatactttg tccttaaatg caactgctac
     5041 gaatgatcga ggaagtttta gtacagtgat aatacccttt gattcctaca tttctggcat
     5101 taattttact accggtagta aagattcggc ttcatgttca acgtttgtct cttaccaggg
     5161 tgggggtatt cctgaaggtg ctaataccaa tgaaattgca gaaactggtt ttgcttcaga
     5221 tggaagtaga tatgcagctc ctctgtatat aatgaattca gaatacatat caatacccaa
     5281 tatgtttatc agaggcgggg atgagttgtt gttgactgtt acaatgaaca cacacgtgtt
     5341 cggagcaaca ggaaatgaaa gttgcctagg aagggcaaaa atctatttaa ttccagctga
     5401 taagtgaatg tagcctattt ttttattttc taactcacca ctacaagtta ttcttgacgg
     5461 tgggttagaa atactgcgga aagcttgctg ttagttcatt tcattacaaa taaaaggatc
     5521 tggtttgcat cgataaaaac ataacccgag acttttgtac tgctgcagtc cggttgatca
     5581 gacaaatact acacttgtgc tttaagctaa tctcctgttg gtattagcct aaccctttag
     5641 caagatcagc tttgatgtct ttgggtgatt ccagtcctac tgcgattcgt agtaatccct
     5701 ccgtgattcc tgcagcgctt ctggcttctt ggcttattct gccatgggtt gtcgtggcgg
     5761 ggtgagttag tgtactttta gcatcaccca agtttgcagt aattgaaatc atgctggtat
     5821 tgtcaatgac gcgccatgcg gcttctctac cgcctttgac ttcgaaggag acgattccac
     5881 cacccgtttt ttgctggagt ttacacagat gatgttgcgg gtgagagggt aggccaggat
     5941 aaaatacgcg tgcaacatta ggctgttctg ttagccagtt tgcaatttct agtgcatgag
     6001 ctgaatgagc ttctattctt atttttagtg tttctagccc ttttaacaaa acccacgcgt
     6061 taaatgcact taaggtgggc ccggcagttc gtaaaaagtt ataaataccc ccatccacaa
     6121 gcaaatcccg ttttcctaaa attgccccgc ctaaaaccct cccttgaccg tctaaatatt
     6181 tggttgctga gtgaatgaca atatctgcgc ctagttctag cggtttttgc aaaatagggg
     6241 tacaaaagca attatcaaca acgagtcgtg cacctgcatt ttttgcaacc tgagcgattg
     6301 cagcaatatc agaaatttct gttaaaggat tggaaggtgt ttcaaggaag aataatttgg
     6361 tgttagcttt acaagcatct gcccattctt gggtttccgt tgcagacaca aacgtggtgg
     6421 taatattaaa tcgactgaga atattgttga aaagattgac cgttgcacca aaaatgctac
     6481 gagaggccac gatatgatcc ccggcggaca ataaaccaag cacacaagca aggattgctg
     6541 acatacccgt ggaagttgca atgcattctt ctgcgccctc aagaacagct aatctttctt
     6601 gaaaggcggt gactgtcgga ttagtgaatc gtgaataaat attgcctggc tcttcacctg
     6661 agaagcgggc agctgcttgc gctgcgctct cgaagacata gctggaggtc aaatacatgg
     6721 cctcagagtg ctcattaaac tgactgcggt gaatgccggt atgaagtgct agggtttcgg
     6781 gatccattat ttgttaaaag tatagtgata atagcgagat gatgataagc gcaaaaaatt
     6841 ccaggattat atgatttagt ccgctagaat taaccctaaa tcgagttgag tcagatcatg
     6901 cttagtaagc ttttttgctc cggcactgcg ctgaaattct aatttatcga gataatcttg
     6961 agttatccct tccgtgatat agtggccatc aaagcaagaa gtttcaaaat tcttgatagc
     7021 aggattgatg cgtgaaatgg aattcttgag cgattcaagt tcttggtaaa cgagaaagtc
     7081 agcaccgatt tcatagcaaa tctgctcttc atctttatca gccgcgatta attcttggcg
     7141 tgttggcata tcgattccat aaacgttggg aaagcgaaca ggaggtgctg cagatgcaaa
     7201 gtaaacctta tgtgctccag cttcttgagc catttgcacg atctctctgc ttgtcgttcc
     7261 tcgtacgatg gagtcatcga ccaataatac attttttcct tgaaactcaa cgttaattgc
     7321 attcaatttc tgacgaaccg atttacgtcg attttgctgg cctggcatga taaaggttct
     7381 accaatgtat cgatttttga caaatccttc tcgaaaatcg attcctaggt gatttgcaag
     7441 ctgtaatgca cttgggcgac tcgattcggg gatcggaata accacatcga tatcgagatg
     7501 ccgcatcgtt tttttgattt tttctgcaag gctgctgccc atattgagcc gagtttcata
     7561 aaccgagaaa ccatccatca tggaatcagg ccgtgctaaa taaacatatt caaaaataca
     7621 aggattaaga cttgagtttg ctgcacattg tttgttatgg aaattacctt tggtatcgat
     7681 gaagatcgct tcaccagggg caacatctcg gattaattta aatccaagtg tatcgagcgc
     7741 gacactttca gacgcaacca tatactcgat accctgttca gtttcagcag ttccaaaaac
     7801 caagggccgg atccctaaac gatcgcggaa ggcgagcagg ccatatcctg cgatcatggc
     7861 gattactgca taagcacctt gacatcgttc gtgtaccccg gctacggcgg caaagatatc
     7921 atctgggtca agctgatcat tcctggcaca agcttgtaat tcatgggcca gaacgttcaa
     7981 taatacttct gaatctgagt ttgtgttcac atgacggcga tcagatcgat ataaggcttt
     8041 attgagttct tctgcgttgg ttaaattgcc attatgtcct agcacgattc caaaaggcga
     8101 gtttacatag aacggttgtg cttcggcagg agaatcagcg gaacccgctg ttggataacg
     8161 tgcatggcct atccccatat tacccagtag tgcacgcata ttacgggtac ggaatacatc
     8221 tcgtaccatt cctagccctt tatgcatata gaaggtattg tcttgagcag ttacgatacc
     8281 agccgcatct tgccctctat gctgcaacat gagcaaacca tcatacaata tttggtttgc
     8341 tggggattgg gctactacgc caataattcc acacataatt gatttaatga gttcaaacgt
     8401 gatgctttag aaaataacgg tatataaaat aattcactta tcaaagctaa tgtggcctcg
     8461 aaaactctca ggtaaccaag gaatgacttg aacggctgct ttctcaagcg ggtcgctcaa
     8521 cacagcttgt tgccaaaaag gctgctttgg taaagcggta aacccagcgg ccaaaactaa
     8581 agacaagaca attgcgaatc ctctcgctaa accgaaaatc gatcctaaca ctctgtcaat
     8641 aaggccaaga cctacacttc ttattaactt ggataataat atagagcaaa acataactaa
     8701 taataaaaca ataaagaaga ccacaacaaa aagcgttagc atccgtaatg agggatcctc
     8761 tatgacgcgc ggtagtaaag gttcaaaaga ttccgcatag cgactggcaa cgtaaaatgc
     8821 gataaaccaa cccgcaagcg atatgacttc cttgatgaca ccacgggtaa ttcccaaaaa
     8881 aacagaaata aacccaatac ctataacaat ataatcaaga attgtcatca atcagtagca
     8941 aatatactca aaggtgctat ttgggatcag cctatcgact cgttacaact ccgcttagcc
     9001 ccattctttt gagtttctca tattcatctt ccgctgcctt tctagtcgaa aagggtccga
     9061 cgcggactct gaccatttca ttatgtccaa ctttaattgt ttcaacgtat gcgccattga
     9121 ttccacttgc agttaaattc tgtagctgct gctttgcttt aagtggatct gaaaaagcgc
     9181 ctaactggat aacaaaaaca ccttcagctg ctttaggttg gggtgtttct ggcgttttgt
     9241 ttaattgagt aactggtttg tgatattgag gcttagataa ggggataggt gtaccttgat
     9301 cgttactaga ttcttcctga gcgtcctcag cagtttgagg cgatccttga ggaatcattg
     9361 ctaataattc gtccggttgc tcggttgaag catcggtttc atcactagaa ggatcagttg
     9421 gcatcatcca cggataattt tctgtcacta aatcttctga aggaatctgg atatctattt
     9481 cctggcgctc ttgtgtcgat tcttcatcaa atagcatcgg tagaaatata actaataaag
     9541 tcactaaggt aattgcaccc aataggcgtc tacgagcacg ttttcttaac aattcttctt
     9601 cttcgctaat atttctgacc attattatta ccctttgatc attaatcaaa aatgatactt
     9661 tactatgtaa acgctaaaat agcacttacg gtgtagaatg aaccaaagac gcatattcta
     9721 tcatttttgc cggcttgctt ttgtgcaaat gcataagcat ccgataaatc agaaaatgaa
     9781 tgaatagcat tgctgtgggt gatgttgctg attatcgatg caatttcttc tgcttgggct
     9841 cctcgcgcag aatgaatggt tccaattagc catacatcga tatggttttt tagtatttga
     9901 ataaccgaag cgatatcctt atcttttaac attccaataa ccgcataggt tttttggaat
     9961 gatggtccca tttgatctaa attttcagca aggacttttg ctgccgctgg gttatgcgcg
    10021 acatctaaaa taacccgagg gcttcccggt aatacttgaa atctaccttt taacgttgtc
    10081 tctaccaggc cacgccgaat cgcttgatta tcaactggga gctcatcttt gagacaatcc
    10141 aatgcggcga ggcaagcaga tgcatttctt aattgaaatg atcctctaag cgagggataa
    10201 ggaagcgcat atacgttatt gtggatccca tagaagttcc attgaagatt gtcttgggtg
    10261 gaataactga aatcctgatt aatcctaaaa aattttgccc ctagctcatc tatcttcatt
    10321 tgtacgctgg gtgggatatc cggttcgctg caaatcgcat agcgatcctt tctaaaaatt
    10381 ccaatttttt ccaggccaat ttcttctctc gtttctccaa gataatccat atgatcaaga
    10441 tcaatgctgg cgagaattgc gcaatctgca tcaaaaatat ttaccgcatc tagacgtcca
    10501 cccaagccaa cttctaatat tgtcacatcc acgtttgatt cgataaataa gtgcatggca
    10561 gctaatgtgc caaattcgaa ataagtgagc gtgacttggc tttgcaaccg agcactttcg
    10621 atagtttcaa aagcgcgaca tatctcggta tcgctaactt cttgttgcaa tatccggata
    10681 cgctcattga aagtgagtaa gtggggggag gtataacaac caacccgata gcctgcggcg
    10741 ctgagcgtcg tttccaacat cgcacagatc gaccctttgc catttgtgcc accgactgta
    10801 attactggaa atgaaggcgt aaggcaaagg ttatctttta cgcgctttac ccgatccaaa
    10861 cctagatcga tcgtaaaggg atgtagattt tcaagatagg cgagccaatc agctaatgag
    10921 cgagattcca cgaaaaggag acaaccttga aggttgagaa atcaaaaaag tgaaaatact
    10981 ccgcgttgct tatttaagag ggaggtagca aggtcgcggc tgatttaggg cttggggttc
    11041 gcatcaaaag cgtgaataaa ttaacgagct tttcacgcat gtggcggcga tctacaatta
    11101 aatcgatagc gccatgttcc aataaaaatt cagcccgttg aaatccctcg ggtaaggttt
    11161 gtcgcaccgt ttgttcaatg acacgtggtc cagcaaaacc aatcaatgca cccggctcag
    11221 cgattacaat atcaccaatg aatgcgaaac tagccgaaac acctcccatg gtaggatcag
    11281 ttaataccga aataaacggt agcttttcct tactcatttg tgtcaatgct gcagaggttt
    11341 tagccatttg catcaatgag aataaacctt cctgcatgcg cgctccgccg cttgcagcaa
    11401 agcaaacaaa cggtaagtgt tgattaatgc tgactcgaac acctctaacg aatcgttccc
    11461 cgacaacaga acccatggag ccgcccataa aaccgaattc aaaggcggca acgacgaccg
    11521 gcgtggtttt tattttacct tgcatcacga cgagcgcatc gtcttcatcg gtgctgctat
    11581 ttgcctgaat taagcggtcg gtatagcgtt tactatctct aaatttaagg gggtcttgtg
    11641 gttttacttc aacaccgatc tcaatacgct cttctggatc aagcagcata tcgatccgat
    11701 ttctagcaga aattcgattg tgatagccac atttggggca aacgttaaaa tttttagcta
    11761 gatcggtata atataataca gcttcgcaag cgatacattt gctccataac ccttcgggta
    11821 caactttctt ttcaccgttt tctttgggtt ttattttggg tggaataatt cgttgaaacc
    11881 agctcattct ttcttgtttt tgtaattata atggtcgacg ttaatttgtc tcatcgatag
    11941 cgctgcgcat agaacttact agattgctta atgtttcaag taatgaattc tcaggtgctt
    12001 tttcgatttc ttcaataata cgactgccga ttactaccgc atccgccagt ttcgagattg
    12061 cttttgctgt ttgtgcgtcc cgtataccga atccaacccc aatgggtata gatactaatg
    12121 ttcggacttg cgctaacttt gtttgtactt cggttagatc aagatgggaa gcacctgtga
    12181 cccctttcaa ggaaacatag taaagatagc cttgcgcaag cttggcaact tgcttcacac
    12241 gctcaatcgg tgttgtggga gacaataaga aaatgggatc aatgccggca gcactaagtt
    12301 gttgtgccca ctcagttgat tcttctggag ggtaatcaac gaccagtatc ccgtcaacac
    12361 ccgattcttt tgcagttgaa ataaaatggt caacgcccat tgcttctacg gggttggcat
    12421 aacccattaa aacaaccgga gtaagtgtat cggtttttct aaattccgcg accatagcga
    12481 ttacatttgc taggcttacg ccatttgcta gcgctcgttc agacgatcgt tgaattgtcg
    12541 ggccatccgc cattggatca gagaaaggaa cgcctaactc aataagatta gcgcctgctt
    12601 ttaccagtgt atgcatcaac ggtacagtta cttcaggttt gggatcgcct gctgtaataa
    12661 atggaatcaa gactttctga tttgcagatt ttaacttgga aaatgttgca gcaattcggt
    12721 tcatttagaa atagtaagtg attttcaatc gctacttaag cgttaaatta aaaagtaatg
    12781 cctgaatatt gagcaactgt catcatatcc ttatcaccgc gtccagataa gtttactaat
    12841 agtaatttat cttttccgag tgtaggggaa agcttggcgg cataagccaa agcgtggctc
    12901 gattcaagcg cagggattat gccttcaaag cgacagagtg catggaatgc tgataatgct
    12961 tcatcatctg tgatcgcaac atactcagct cgattactat ctttaagcca tgcgtgctca
    13021 ggacctacac caggataatc taaaccagca gatatcgagt gggtttcaat aatttgacca
    13081 tgatcatctt gaattaaata agttcggtta ccgtgcagca caccgggtcg gcctgttact
    13141 agcgtggcag catgttgatt cgaattaatt ccttttccac ccgcctcaac accaatcata
    13201 cgaactgtct catcatcgat ataaggataa aacaaaccaa gcgcattgga tccacccccg
    13261 acacatgcaa taagtgcatc aggctggcgt ccatactctt gctgcatttg atcctttact
    13321 tctttaccaa taattgcctg aaaatcccgc accatcatag ggtagggatg agggccagct
    13381 actgttccga taatataata agtgctttcc acattggtaa cccagtcgcg cattgcttca
    13441 ttgagggcat ctttgagtgt acgggagcca gactcaacgg gtatgacttg tgcacctagc
    13501 aatttcattc tataaacatt cattgcttgg cgtttaacat cttcagaacc catataaacg
    13561 acacattcca tgccttgacg tgctgctact gtcgctgttg ccacaccatg ctgaccggca
    13621 cctgtttcag caatgacccg ttttttgttc atacgctttg ctagtagcgc ttgaccaact
    13681 gtattattga tcttgtgcgc gcccgtatga tttagatctt cacgtttgag aagaatctga
    13741 gcgcctccta aatggtcaga ccaacgtttt gcatgataaa taggggtagg acgcccaacg
    13801 tagtgtttga gctcataagc aaactctgtt tgaaacgctt tatcagcttg ataataaaga
    13861 tattgagtcc gcagctcctc cagcgctgtc atgagggttt cggccacaaa tttaccgcca
    13921 taaggaccga agtggccttt ctcatcagga agattatatg cactcacaat ttttaaccct
    13981 ttgcataaac gcagaaattt tattgatatc cttaatacct ttgcttgctt caacaccact
    14041 tgatacatca acagcccatg gttttacctt cgctattgca tgaactacat tatcaggagt
    14101 taatccgcca gacaatataa ttggaagcgg gagattggct ggaatcaatg tccaatcaaa
    14161 aacttcacct gttccacccg gcagattggc actataagta tctaacagaa gcgcttttgc
    14221 actttggtat cgatttgcgt attctagcag atccatatct tccttgatac gaagcgcttt
    14281 aacataaggg agcgagaatt gatcacaaaa ctcgggagac tcatttccat gaaattgtaa
    14341 caaacctaaa ttagcgatgg ttgctgtttc atggacccac tcaacggttg ggtcaacgta
    14401 aacccctact gttgttacaa atgcaggtat ggttttagtg atgggtcttg cttgttgagg
    14461 caagatgttt cgagcgcttt gctgccaaaa gacaaaacca atagcatcgg caccagagga
    14521 tattgctgcc agtgcatctt cagttcgtgt gataccacaa atttttactc gagtacgcat
    14581 gtgagtttta ttgttattat gtttaatacg ttgctcagtt gttggtttat ttttttataa
    14641 atacctctaa ggccgtgatg tcgcaaagtg aatcatttaa tgtaggaaga ttccagcgag
    14701 tatcatattt tacctcagct aagtataagc catatgggga aaaagttggt gctgcacgtt
    14761 tccggtcacg actttctaat actgcggtga tccagctggg ttgatggcga tttgcgccga
    14821 cataaatcaa gctaccaacg atattacgga ccatgtgatg taaaaaaccg tttgccctga
    14881 attcgaattt taagaaatcg ccttgttgcg ttatcttaag ttttgtcagt ttacggacgg
    14941 gatttttggc ttggcactca gcggtacgaa atgcactaaa atcatgttct cctagtaagc
    15001 tgtcggctgc tttttgcata tcatcgacat tcagtggttc atgaaaccaa cc
//
LOCUS       DAOQLJ010000045        14803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1477_length_14803_cov_5.944738, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000045 DAOQLJ010000000
VERSION     DAOQLJ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 14803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14803
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1477_length_14803_cov_5.944738"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /gene="yegQ"
                     /locus_tag="PK522_04840"
     CDS             <1..1135
                     /gene="yegQ"
                     /locus_tag="PK522_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HNC41092.1"
                     /translation="RKVKTYMADMEPVIDLKPDALIMADPGLIMMVREKWPEIPIHLS
                     VQANTVNYAAVKFWQKIGLTRIILSRELSLEEIAEIRQLCPDIELEVFVHGALCIAYS
                     GRCLLSGYFNHRDPNQGTCTNSCRWDYKVKPAQENPEGDIQALQTIDFDFNQAVSQSA
                     LDDASPIRHPAADHVYLIEEKERPGQLMPILEDEHGTYIMNSKDLRAIEHIEQLIHIG
                     IDSFKIEGRTKSAYYVARTAQIYRKAIDDAVAERPFDYTLLGQLENLANRGYTAGFYQ
                     RHASQETQNYLRGHSESNQSQYVGDITTSDESNGMVEILVKNRFGIGDRLEIIHPSGN
                     RIIELDYMQDHNGKTIDVAPGSGHRVRIPLTGNVEKAFIAKLL"
     gene            complement(1234..2496)
                     /locus_tag="PK522_04845"
     CDS             complement(1234..2496)
                     /locus_tag="PK522_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HNC41093.1"
                     /translation="MLRFDRITLILLIFFFSEAYAVGWEKIEVSNTELTILDDNGQYK
                     DIKPGCAFSYLPDEAGLPNKPFHFYYRKGTKPQTLIFLNGGGACWNDATCTTSLTVPV
                     SSTTRPAYNPSMDFENDPESVGGILDFARADNPLKDWNMVFIPYCTGDAHIGSNDKVY
                     ADPLGIINAGNPIMVQHRGFDNFMAVREWLKHRPDRFNTEKVLVAGSSAGAYGALMNF
                     SRLHRIYPSMTKISLLSDAGAGVFTNNFLNTVFEPNGPWGTENTLATWIPGINQISSY
                     NDLNFFTNLATGIERYFYRSKFAYVTTAWDGVQVLFLNIMKKTDQGINDPNEWFNLTP
                     EVFVEWNSRMLASFSVNVLFNSNAKYYVSAGTYHAGLVDVFVPDIFYTENSASGIFLK
                     DWVNRLVINDKKYPLINLMCSGTCGAPF"
     gene            complement(2614..3606)
                     /locus_tag="PK522_04850"
     CDS             complement(2614..3606)
                     /locus_tag="PK522_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC41094.1"
                     /translation="MKSTKYNPPRWLWGGNAQTIYPYLMSQPASIPYRRERWELDDGD
                     FIDVDWLDGEGNKPLVVLFHGLEGGSRSHYALSMMRYLQSCQWNGVVIHFRGCSGYPN
                     RLPRAYHAGDSAEIDWMLKRIASIQSSQPQKSNRTIYAMGVSLGGNALLKWLGEQGKI
                     ANQHIAAVAAISVPMDLVAAGNVLDKGFNQLYTRHFLSSLKQKIVGKQKKFPGLFDIK
                     AVTQCATLYEFDNLVTAPLHGFRDTDEYWTTSSSKPWLRAIEVPTLVLNAKNDPFLPA
                     HALPEYSAVSSAVTLEYPEQGGHVGFLHGPFPGKLMWLPERILRFFEGVNCNNQ"
     gene            3667..3743
                     /locus_tag="PK522_04855"
     tRNA            3667..3743
                     /locus_tag="PK522_04855"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3701..3703,aa:Val,seq:gac)
     gene            complement(3815..5191)
                     /gene="phoR"
                     /locus_tag="PK522_04860"
     CDS             complement(3815..5191)
                     /gene="phoR"
                     /locus_tag="PK522_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HNC41095.1"
                     /translation="MFEIGRRFVGIVLLIIVSVILGFFFDETVALLFLSSILLIIVSY
                     HIISLASLDRWLQRYDPSSQVMASVPNNFGAWGDVFVRLTRYMRRYYRSRQSLSRALE
                     RLQRATSAMPDGIVILDKIDRIEWCNPVAEQHLGLDLKMDIGQHITHLVRQMQFVEYL
                     TSGDFSKPVIIKQSRYQEIMLSLQLVPYGDEEKLLISRDITRFEKMEIMRRDFIANVS
                     HELRTPLTVINGFLEMLQDNGMKDSDMEKHALGLMSQQSRRMQRLVEDLLTLSRLENG
                     QNVLREEEVDMKELLQNLLHEAQSLSAARHTITLQLKKDDINVLGSADELRSALGNLV
                     SNAVRYTPDGGDIVLSWGEEKGLFYVRDSGIGIEPQHIPRLTERFYRIDRSRSRETGG
                     TGLGLAIVKHILNRHQARMDIISELGKGSTFKVWFPLKRLQATTKNSLPEVVEAQLEH
                     SQFMPPQQ"
     gene            complement(5463..6188)
                     /gene="phoB"
                     /locus_tag="PK522_04865"
     CDS             complement(5463..6188)
                     /gene="phoB"
                     /locus_tag="PK522_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HNC41096.1"
                     /translation="MAATILIVEDEPAIQELISYNLRNTGFIVACAGSAEQAKIMVND
                     VLPDLILLDWMLPGTNGIDFARYLRQGARTKAIPIIMLTARVDESDKVSGLEVGADDY
                     VIKPFSPRELVARIKAVLRRRLPEASDEIVETGGLKLDPATHRVMVTDSADKSKSIEV
                     NLGPTEFRLLHYLMTHTERVHTRGQLLDRVWGDHVFVEDRTVDVHIRRLRRALEDVGK
                     DDLIQTVRGAGYRFSYQCEVDTH"
     gene            6303..6674
                     /locus_tag="PK522_04870"
     CDS             6303..6674
                     /locus_tag="PK522_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNC41097.1"
                     /translation="MAGLNKNTPTPTEIKFHKQSKLLEITFNNNQKFQLSSEFLRVYS
                     PSAEVQGHGPGQEILQTDKQDVTIVKIEPIGQYAIQPHFSDGHNTGIYSWDLLYNYGV
                     NQEKMWHHYLQRLEAAGIKHD"
     gene            6716..7450
                     /gene="ubiE"
                     /locus_tag="PK522_04875"
     CDS             6716..7450
                     /gene="ubiE"
                     /locus_tag="PK522_04875"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNC41098.1"
                     /translation="MTNTTHFGFKTVEESEKAHKVADVFHSVAARYNLMNDLMSMGLH
                     RLWKRFAIDASGAKKGDRILDIAGGTADLSALFLEQVGESGQVWLTDINNSMLTIGRD
                     RLLNDGKAVPATQCDAEKLPFPDNYFDHVCVAFGLRNMTHKDIALKEMLRVLCPGGSV
                     IVLEFSKIWKPLQPLYDAYSFKALPFIGKFVAQDEESYRYLAESIRMHPSQEELKQLM
                     QDVGFERVEYFNLSTGIVALHRGYKF"
     gene            complement(7407..8357)
                     /locus_tag="PK522_04880"
     CDS             complement(7407..8357)
                     /locus_tag="PK522_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNC41099.1"
                     /translation="MKLYQYTFKAMGTPCEIQLYGAVESEVSQVAESAIADVRRLETA
                     YSRYRDDSFLSEINRVAAHGGSIQVDSETAGLLDYAEACYHASDGLFDVTSGILRQAW
                     NFKSNQLPDEQTIQSLLDRVGWEKVHWLSPNLEFPVAGMEIDLGGVVKEYATDRAASI
                     CLDRGIQNGMVNLGGDIKVIGPHIDGSPWKIGISHPRQPATLLHTLALHSGALASSGD
                     YERCITINGIRYGHVLNPKTGWPVRKMAAVSVVGDFCVVAGSASTIGMLKDEEAKDWL
                     AILGLPHLWVNVSGEVGGSLLDTVQVTKIYNHDEAQRYPY"
     gene            complement(8363..10903)
                     /locus_tag="PK522_04885"
     CDS             complement(8363..10903)
                     /locus_tag="PK522_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HNC41100.1"
                     /translation="MSKTLKTLTSAALLLPGLVVHTSQAADPSRANINFQHTRYQEGK
                     RDLLGIRSDLNPIMVDTLHGTGGFLLTDRLRFSFGYTQDTWSGATPIAMTPFASTAGN
                     KEFTRTLPTGEVVSGASPYITGIVDLDRNLDPLRQDPVAGRVVDPRTVLVLSTASPET
                     RKQGDFRMGYEWDNAVVNFGGGLSHENDYKSSYGNVNGRFDFNQKLTSLKWGGAYTTS
                     ATSAILDHDTLSYVTPLAYNGQIDFQDRLRILRGSRQDWAANIGLTQVLGKSSLIDAN
                     IGYTHSSGFMENPYKATTVIFIDPDTISPNPADSIRGNVRAVLEQRPDYRNQLAISNR
                     FVQHISPLDAALHLNYKFSFDDWGINAHTFESDWIQPLFEGWTLTPRIRYYSQSAADF
                     YQPYLFSNQAFLKTVTNSAGETQAITYDPRKLPQDFASDHRLASFGSLSGGISLNKRF
                     ARGFELEAGFEYYTRASAFAFGGGNSSFADFDYYVANAALKIGLETLNVNQIGRNRSA
                     PGAAHDHTAQHDHAFIPAGIMFGHTLDKAGDIMVGYRFMYARQTGDILRGSHVVSDLE
                     IVNQACGDLVLCRFAPDYMNMRMHMLDIMYAPTRWLNLMLMPQFMDMEMNLRPLEGRP
                     PLAPGGHDHPGTLHATGGVADTIMMPLIKLLDQPNHRLHIGLGFSAPTGSVDQEVRRM
                     AKIDGGIIHFDMQLGSGTWDFMPNLTYTGSHSQWYWGGQLYGVKRLENKNESGYRLGD
                     LFQASTWGGYRLTNWLAASVRGVYTTRGAIHGDFNTFNARIGPMDFPHNQGGQFWDLG
                     IGINARVPSGRLVGNHFAFEWLQPLRDDFNGYQLERQGALSATWHYSF"
     gene            complement(10951..11169)
                     /locus_tag="PK522_04890"
     CDS             complement(10951..11169)
                     /locus_tag="PK522_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HNC41101.1"
                     /translation="MRRSTFILFLFISFLLAGCVNVAPWERGILAKPHMALDPQPLQS
                     GIRQHNYGSRESAASTNSAGGGGGCGCF"
     gene            complement(11199..11735)
                     /locus_tag="PK522_04895"
     CDS             complement(11199..11735)
                     /locus_tag="PK522_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNC41102.1"
                     /translation="MKISNKFYGLTLAVTLTCFAVSPSFAHLEGDVPPPQCKLTALDG
                     KKVEDWSTLKGEVVYVDFWASWCPPCIQSFPFMTQLMDEFKEKGLHVIGVNLDENSQD
                     AINFLKQHPVNFSVLMDETKRCAEDFGVIAMPSTYIIDKNGLVRHIHRGFRAGEMQEL
                     RHLIEQLLAENVTLGKVN"
     gene            complement(11788..12270)
                     /locus_tag="PK522_04900"
     CDS             complement(11788..12270)
                     /locus_tag="PK522_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41103.1"
                     /translation="MKQSTVFNVRKLVKQCITVLALSGGMLGVIHTGLAHDAAATMDS
                     VGNSATFTGYGFVTCFDDGNGPADKLVASVRDHSPPVPGLLVNLQILKGNRAASVSDP
                     VSGDGNGSPSISVKGGNGVYLILVNKTAPGARDFSIEYHCTTANDVHTGTDIGVYQFE
                     "
     gene            complement(12353..13096)
                     /locus_tag="PK522_04905"
     CDS             complement(12353..13096)
                     /locus_tag="PK522_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41104.1"
                     /translation="MKQMTKARALVLSAVSAALVGMGMSAFAHTRLDIPTMTEGSRTI
                     NHLVGHTCGSNKAIGFSVVFPDGVDSTVLVNSQPHTGALTDFVEGYGNNAQLIFDRSQ
                     FDIVDEKMDPSGANVVGFWGGGGPGVPSNLTVATRFRLTAPTIVPTSCAKSVKAYISI
                     AVVCQVTGTDQFGTEGVVDLWTPNNLGTPFDSADANADAPWTISRDLTKNPLPESCGE
                     GVDVEIKPSAAQINRDMPVKVNGQQVWPQ"
     gene            13611..>14803
                     /locus_tag="PK522_04910"
     CDS             13611..>14803
                     /locus_tag="PK522_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNC41105.1"
                     /translation="MQPTQHNSFSNFWLYCTVFLTGAAVMIIELLGTRLIAPFYGASL
                     YVWASLISVTMIALAMGYFLGGYWADRAKHAGLALIIGLAALLTMTIPWLVQPVLLST
                     DHLGLKLGTFISTLILFTPSLTMLGMVGPFAVKRITANLAGVGTSTGSIYAVSTVGSV
                     IGTLFLGFYLFPQIGSREIIIGLAVGLFVLAFIVAIFERHQIPLIKTLSFVACFFFMG
                     VSILLATTPLSNERVNQQDNDYQTRFERESLYGWVRVIDNPSLNLRLLTADASTIGAA
                     SISQGENVLTYQKIVKMIPNLVPNMKQALLIGQGAGHMATTLERKGIRTDTIEIDPAV
                     AEAARDYFDFIPTGQTLIGDARYEIRKLKGPYDLVILDVFTGGSEPVHLLTFETLEQI
                     KALLTP"
BASE COUNT         4276 a         3597 c         2855 g         4075 t
ORIGIN      
        1 ccgtaaagtc aaaacctata tggcagatat ggagcctgtc atcgatttga aaccggatgc
       61 cctgatcatg gctgatccag gtctaatcat gatggtacga gagaaatggc cggaaattcc
      121 cattcacctc tcagttcaag ccaatacggt aaattatgct gccgttaagt tctggcaaaa
      181 aattggcctc actcgtatta tcctatcacg tgaactctct ctggaagaaa tagctgaaat
      241 ccggcagcta tgtcctgaca ttgaactcga agttttcgtc catggcgcat tatgcatcgc
      301 ctactcagga agatgtttgt tatctgggta ttttaaccac cgcgacccca accaaggtac
      361 ctgcaccaac tcctgccgtt gggactataa agtcaaaccg gctcaagaaa atccggaggg
      421 tgatattcaa gcgctgcaaa cgattgactt cgactttaat caagcagttt ctcagtctgc
      481 attggatgac gcttcgccaa ttcgccaccc tgcagccgat catgtttatt tgattgaaga
      541 aaaagaaagg cctggtcaat tgatgcccat tctagaagat gagcatggaa cctatatcat
      601 gaactcaaaa gacctacgcg cgattgaaca tattgagcaa ctcattcata ttggaattga
      661 ctcattcaaa attgagggcc gtactaaatc agcctattac gttgcacgaa ccgcacaaat
      721 atatcgaaaa gccattgatg atgctgtcgc agaacgtccg tttgattaca ccttgcttgg
      781 tcaactcgaa aacctggcga atcgcgggta tacagctggt ttttatcagc gccacgcaag
      841 tcaagaaact cagaattacc tacgcggtca ctccgaatcc aatcaaagcc aatatgttgg
      901 agatatcact acttcagatg aatccaacgg aatggtcgaa attcttgtta aaaaccgttt
      961 tggtatcggt gatcggctgg aaatcattca cccatcgggg aatcgtatta ttgagctcga
     1021 ctatatgcaa gatcataacg gaaaaactat tgatgttgca ccagggagcg gccatcgcgt
     1081 tcgtatccca ttaaccggaa atgttgaaaa agcctttatt gcaaaattac tctgatacta
     1141 ctactccagt agttttaaat tttgtgtagt ctctggtaac aaataccaga gactacacct
     1201 atacctctct cagaaagtga ttactttctt tccttaaaaa ggcgctccac aggtaccact
     1261 acacatcaaa ttaatcaaag gatacttttt atcattgatg actaaccgat taacccaatc
     1321 ttttaagaaa ataccactcg cactattttc agtgtaaaag atatcgggta cgaacacatc
     1381 aaccagacca gcatgatagg ttccagcact tacgtaatac ttagcgttgc tattgaataa
     1441 tacattcacg ctaaatgaag ccaacattct cgaattccat tcaacaaaga cttcaggtgt
     1501 caaattgaac cattcgtttg gatcattgat gccctgatct gtttttttca taatattgag
     1561 aaataacact tgaacaccat cccaagcagt cgtgacatac gcaaatttac ttctgtaaaa
     1621 ataacgttca atacccgttg ccaaattagt aaaaaaattt agatcgttat agctactaat
     1681 ctgattgatt cccggtatcc aagtcgctaa ggtattctct gttccccatg ggccgtttgg
     1741 ctcaaaaacg gtattcaaaa aattattggt aaatacaccg gcacccgcat cagacaatag
     1801 agaaattttt gtcatgctcg gataaatacg atgtaatcga gaaaaattca ttagtgctcc
     1861 gtatgcgccg gcacttgacc cagctactag cactttttca gtattgaatc gatccggtct
     1921 atgctttaac cactcgcgca ccgccataaa attatcgaaa ccccggtgct gtaccatgat
     1981 aggattacca gcgttaatga ttcctaaagg atcagcataa actttatcgt tcgaaccaat
     2041 atgtgcgtca cccgtacaat agggaataaa gaccatattc caatctttca gcgggttatc
     2101 tgcacgagca aaatcaagta ttccccctac actttctgga tcattctcaa aatccatgga
     2161 aggattatag gcgggccgtg ttgtgcttga aactggaacc gttagagatg ttgtacaggt
     2221 tgcatcattc cagcaggcgc ctcccccatt aaggaaaatc agtgtttgtg gcttagttcc
     2281 tttccggtaa taaaaatgaa atggcttatt cggtaatcct gcttcatcag gtaaataact
     2341 aaatgcacaa cctggcttga tatctttata ctgcccgtta tcatccagta tcgttaactc
     2401 agtgttggat acttctatct tttcccatcc taccgcatac gcttctgaga aaaagaaaat
     2461 cagtaatatc aaagtaattc gatcgaacct tagcatatca cctccccttc ggtagattga
     2521 ctatcaatat cgatattcac ggccataact tctgtaaccg ttagatccca tgataatact
     2581 aagtcataaa ctttggtaaa agaaacttat aagtcactga ttattgcaat ttaccccttc
     2641 aaagaaacga agtatacgtt cgggcagcca catcaattta ccgggaaatg ggccatgtaa
     2701 gaaaccaaca tgccctcctt gttcgggata ctccagcgtt actgccgaag aaaccgctga
     2761 atactcaggt aatgcatgtg ctggtaagaa aggatcattt ttcgcattca gtacgagcgt
     2821 gggtacttca atcgcccgta accagggttt actactcgat gtcgtccaat attcatcggt
     2881 atcacgaaat ccatgtaagg gtgccgttac caaattatcg aattcatata acgttgcgca
     2941 ttgagtaact gctttaatgt caaataagcc tggaaatttt ttttgtttac ccactatttt
     3001 ttgctttaag gagcttaaaa aatgccgcgt ataaagctga ttaaatccct tatccaatac
     3061 attgccagca gcaaccaaat ccatgggaac tgatatcgct gccacagctg caatgtgctg
     3121 attcgctatc ttcccttgct cccctagcca ttttaatagc gcattgcctc ccagagaaac
     3181 gcccattgca tagatagtac gatttgattt ttgtggttgt gaggactgta ttgaagcaat
     3241 acgctttagc atccagtcaa tttctgcaga atctcccgca tgataagcac gcggtaaccg
     3301 attcggataa cctgagcatc cacgaaaatg aatgacgacc ccattccact ggcagctctg
     3361 taaatagcgc atcatactca acgcgtaatg gcttcgcgat ccaccctcta atccatgaaa
     3421 caatacaaca agtggcttat ttccctcgcc atctaaccaa tccacatcaa taaaatctcc
     3481 atcatcaagc tcccatcgct ctcgccggta gggaatcgat gctggctggc tcattagata
     3541 gggataaata gtctgtgcat taccgcccca aagccatcgg ggaggattat attttgtcga
     3601 tttcattgat gtttaaacaa taaatttgaa gcggattttc ttttcagtta gaatgcaagc
     3661 acgcttaggc gcgtagctca gctggttaga gcatcacctt gacatggtgg gggtcgttgg
     3721 ttcgagtcca atcgcgccta ccatcgaggg aattaatcca aatcacccta attccccaca
     3781 tctcagtcat ttccacctaa cggttaatta attattattg ttgcggtggc atgaattggc
     3841 tatgttccaa ctgtgcttcc accacttctg gtaagctatt tttggttgtt gcttgtaatc
     3901 gttttagcgg aaaccacact ttaaacgtac tcccttttcc taattcactg ataatatcca
     3961 ttcgcgcttg atgtcgattc aaaatatgtt tgacgattgc taatccaagc ccagtccctc
     4021 cagtttcacg tgaacgactc cgatcgattc gataaaatcg ttcagtcaag cggggtatat
     4081 gctgaggttc gataccgatg ccactatctc gaacataaaa taaacctttc tcttctcccc
     4141 aagataaaac gatatcaccc ccatccgggg tataacgcac agcgttactg actaaattcc
     4201 ccaacgcact tcgcaactca tccgcgctac caagcacatt gatatcatct ttttttaact
     4261 gtagggtgat ggtatggcgc gctgcactta aagattgagc ctcatgaagg agattctgga
     4321 gtagctcttt catatccact tcttcctcac gcaaaacatt ttgtccattt tccaaccgcg
     4381 ataacgttag caagtcctcg accagtcgtt gcatgcgccg actttgctgg ctcatcaatc
     4441 cgagcgcatg tttctccata tcgctatctt tcattccgtt atcttgaagc atttcaagaa
     4501 atccattgat taccgtcaga ggcgttctga gctcgtgcga gacattggca ataaaatccc
     4561 gacgcatgat ttccatcttc tcaaatcgcg taatgtctcg actgatcaac aatttttcct
     4621 catccccgta aggcacgagc tgtaacgata gcatgatctc ctgatagcgc gattgcttaa
     4681 ttataactgg tttactaaaa tcccccgaag tcagatactc cacaaactgc atctgtcgca
     4741 ctaaatgcgt aatatgctgc ccaatatcca tttttaagtc taatcctagg tgctgctctg
     4801 caacaggatt acaccattca atccggtcga tcttatccaa aatcacaatt ccatccggca
     4861 tggctgaagt ggccctttgc aggcgctcta atgcccggct caagctttgt cggctacgat
     4921 aataacgccg catatagcgc gtcaaccgca caaaaacatc gccccaagca ccaaagttat
     4981 taggaacgga agccattact tgagaagacg gatcatagcg ctgtagccaa cggtctagtg
     5041 aagccaatga aatgatatgg taactgacaa tgatcagtaa gattgaactt aagaaaagca
     5101 gtgctaccgt ctcatcaaag aaaaatccaa gtattacgga aacgatgatc agcaatacga
     5161 tcccgacaaa tcgacgccct atctcaaaca cgtattgatt ccaaagaaag gtagggtttg
     5221 gttacagaat ctcgaatttc catttattct gacgttagga tagctaagaa atcgatctga
     5281 aatgcaggta gatcattgct catatttttg acaagcaatg acttctaaac aatgagcatt
     5341 taacgctaaa aattatcaag gcgacttaaa ttgactaagg cgctatatag tagcttaagt
     5401 ctaagtcggt cgtacactat ttcattcaac caaccgccgt actttattaa atgatatacc
     5461 gattaatgag tatcaacttc acattggtaa gaaaaccgat aaccagcacc ccgcacagtt
     5521 tgaatcaaat catccttacc tacatcttcc aaagcacgac gtaagcgacg tatatgaaca
     5581 tcgaccgttc gatcttctac gaaaacatga tcaccccaaa cgcgatccaa taactgtcct
     5641 cgcgtatgta cacgttccgt atgcgtcatt aaataatgca ataatcgaaa ctccgtcggc
     5701 ccaagattga cttcgattga ttttgattta tctgcgctat cggtcaccat cacacgatga
     5761 gtggccggat ccaactttaa cccccctgtt tcaacgattt catctgaggc ctctggtagc
     5821 cgtctacgta acaccgcttt aattcttgca accagctcac gcggtgaaaa cggtttgatt
     5881 acataatcat ctgcaccgac ttctaaccct gatactttat cactctcatc aacccgagcc
     5941 gtcagcatga taatgggaat cgctttggtg cgggcacctt gccgcaaata tcgcgcaaaa
     6001 tcaataccat tcgtcccagg aagcatccaa tccaataaaa ttaaatcagg tagcacgtca
     6061 tttaccatga tctttgcttg ctcggcactt cccgcacacg ctacaataaa gcccgtgttg
     6121 cgcagattat aagaaattaa ctcctgtatc gcaggttcat cttctacaat taaaattgtt
     6181 gcagccatcg attgactcca ctcaaaaata agtttttagt aagtctctca atagtataaa
     6241 gctaacattg caactttgtg acaaatacaa atttgtcatc taaaactaat tattaatttt
     6301 ttatggctgg cttgaataaa aacacaccaa ctccaacaga aataaaattt cacaaacaat
     6361 cgaaattgct tgaaatcacg tttaataaca accaaaaatt tcagttatcc agtgaattct
     6421 tgcgggttta ctcaccttct gctgaagttc agggccatgg gccaggacag gaaatactcc
     6481 aaactgataa gcaagatgtc accattgtga aaatcgaacc cattgggcaa tatgccattc
     6541 aaccgcattt ttcggatggg cataatacgg gaatctattc gtgggattta ctttataatt
     6601 acggggtcaa tcaagaaaaa atgtggcatc attatcttca acgactcgaa gctgcaggta
     6661 tcaagcacga ttaattcgtc cgcttttcct tgggctccct tatatttaaa tagacatgac
     6721 caatacgact cacttcggtt ttaaaacagt tgaagaatct gagaaagcac ataaagttgc
     6781 cgatgttttt cactccgttg ccgctcgcta caacctaatg aatgatctca tgtcaatggg
     6841 cctacatcga ctctggaaac gttttgcaat tgatgccagt ggcgctaaaa aaggagatcg
     6901 tatacttgat attgcgggcg gcacggctga tttgtcagct ttatttcttg agcaagtcgg
     6961 tgaatccggt caagtatggc taactgatat taataattcc atgctgacca tcgggcgtga
     7021 tcgtttgtta aacgacggca aagcagtccc tgccactcaa tgcgatgcag aaaaactacc
     7081 atttcctgat aactattttg atcatgtatg tgtcgccttt ggtcttagaa acatgactca
     7141 caaggatatt gcactcaagg aaatgttacg cgtactgtgc ccagggggtt cagtcatcgt
     7201 attggaattt tccaaaatct ggaaaccact acaaccgctt tatgatgctt actcattcaa
     7261 ggcgttgcct ttcattggaa aatttgtagc gcaagacgaa gagagttatc gatatctcgc
     7321 agaatcgatt cgcatgcacc cttctcaaga agagttaaaa caactcatgc aagacgttgg
     7381 ctttgaacgc gttgaatatt ttaatctcag tacgggtatc gttgcgcttc atcgtggtta
     7441 taaattttag tcacttgcac tgtatccagc agcgacccac ctacttcacc cgaaacatta
     7501 acccataaat ggggtaagcc aaggatagca agccaatcct tcgcttcttc atcttttaac
     7561 ataccgattg ttgaggcgct tccggcaacc acgcagaaat caccaacgac acttactgca
     7621 gccatttttc taactggcca acccgtttta ggattcaata catgcccata acggatacca
     7681 ttaatcgtaa tacatcgctc ataatctccg ctactggcca aggcacccga atgtaacgct
     7741 aatgtatgca gcaaagttgc gggttgtcgt gggtggctta taccaatttt ccagggactt
     7801 ccatcgatat gaggaccgat gactttaata tctcccccta aattaaccat accattttgt
     7861 attccacgat ccaaacaaat cgatgcagca cgatctgtcg catattcctt tacaacaccc
     7921 cctaaatcga tttccattcc agcaacggga aattcaagat tgggcgataa ccagtgcact
     7981 ttttcccaac ctactcgatc caataaagac tgtatcgttt gctcatccgg caattgattc
     8041 gatttaaaat tccaggcttg tcttaatatc cctgaggtaa catcgaatag cccatcactc
     8101 gcatggtagc aagcttctgc gtaatccaat aatcctgcag tttccgaatc tacctggatc
     8161 gatccaccat gagcagcaac acgattaatc tctgataaaa agctatcatc acgataccgt
     8221 gaatacgcgg tctccaatcg acggacatcc gcaatggcgg attcggcaac ctgggatacc
     8281 tcgctttcca cggctccata aagctgaatc tcacaaggcg tgcccattgc cttaaatgta
     8341 tattgataaa gtttcatcac catcaaaaac tataatgcca cgttgcggat aaggccccct
     8401 gacgctcaag ttgatagcca ttgaaatcat cacgcaacgg ctgcagccat tcaaatgcaa
     8461 aatgattgcc caccagtcgt ccactcggaa cccttgcatt aatcccaatt cctaaatccc
     8521 aaaactgccc accctggtta tgtggaaaat ccattgggcc aatgcgtgca ttgaatgtat
     8581 tgaaatcccc atgaatagcc ccccttgtcg tataaacacc gcgcacggac gccgctaacc
     8641 agttcgttag acgatagcca ccccaagtcg aggcttggaa aagatcaccg agccgataac
     8701 cggattcatt tttattctct aaacgcttca caccatatag ctggccaccc caataccatt
     8761 gggaatgact cccggtatag gtcagattcg gcatgaaatc ccaagtccca cttccgagct
     8821 gcatatcgaa atggatgata ccgccatcaa tcttagccat acgtctgact tcttgatcaa
     8881 cacttccggt aggtgcgcta aaccccaacc cgatatgaag tcggtggtta ggctgatcga
     8941 gaagcttgat cagtggcatc atgatggtat cagccacccc gccggttgcg tgcagtgtac
     9001 cgggatgatc atgccctccc ggtgcaagcg gtggtcgacc ttctagcggg cgaagattca
     9061 tctccatatc catgaattgg ggcatcagca tcaaattcaa ccagcgggtt ggcgcataca
     9121 tgatatccaa catatgcatc cgcatattca tatagtcagg cgcaaaacgg caaaggacca
     9181 aatcaccgca agcttgattg acaatttcca aatcactaac aacatgtgat ccacgcaaaa
     9241 tatcgcctgt ttgacgcgca tacatgaaac ggtaaccgac catgatatcg cccgctttat
     9301 cgagtgtatg accaaacatg atccctgcgg gaatgaaggc atgatcgtgt tgtgctgtat
     9361 ggtcgtgcgc tgcacctggc gcagaacggt ttctgccgat ctgattcaca ttcagtgttt
     9421 caagtccaat tttaagcgct gcattagcaa catagtaatc aaaatcggca aaactactat
     9481 tccctccccc aaatgcaaac gcactggcac gtgtatagta ttcaaacccc gcttcaagct
     9541 cgaaaccacg tgcaaatctt ttattcaggg atattccccc actcaaagag ccaaaacttg
     9601 ccaagcgatg atcgctcgca aagtcttggg gcagtttccg gggatcgtag gtaatggctt
     9661 gagtttcacc ggcgctattc gtaaccgttt ttaagaatgc ctgatttgaa aaaagatagg
     9721 gctgataaaa atcagcagca ctttgtgagt aatagcgtat ccgtggggtc aacgtccaac
     9781 cctcaaacag cggctgaatc cagtcgctct caaaggtatg cgcattaatc ccccaatcat
     9841 cgaatgaaaa tttatagttc aaatgtaacg ctgcatccaa aggactgatg tgttgcacaa
     9901 accgattact gattgccaat tgatttctat aatcaggtcg ttgctctaac acggctcgca
     9961 cattgccgcg aatggagtca gcggggtttg ggctaatggt atcaggatcg ataaagataa
    10021 cggtagtggc cttataggga ttttccataa aaccactgct atgggtatac ccaatattgg
    10081 catcgatcag tgaacttttt cccaacactt gagttaatcc gatattggcg gcccaatctt
    10141 gccggctacc ccttaaaatt cttagtcggt cttgaaaatc aatttgcccg ttatacgcaa
    10201 gcggtgtgac ataactcaac gtatcgtgat cgagaatcgc ggaagttgca ctcgtggtat
    10261 aagctccgcc ccatttcaaa ctggtcaatt tttgattgaa gtcaaaacgg ccattgacat
    10321 ttccataact cgatttatag tcattctcgt gcgataaacc acctccgaaa ttcacgactg
    10381 cattgtccca ctcgtaaccc attctaaaat ccccttgttt gcgggtttct ggggatgcgg
    10441 tcgatagcac taatacagta cggggatcga cgacacgccc tgcaacgggg tcttgtcgaa
    10501 gcggatctaa attgcgatcc aaatctacaa taccggtaat ataaggagat gcgcctgaaa
    10561 ccacctcacc ggttggtagc gtgcgggtaa actctttatt accggcagtc gatgcaaacg
    10621 gtgtcatcgc aatcggggtt gcacccgacc aggtatcttg cgtgtaacca aatgaaaacc
    10681 gaagccgatc ggttagtaga aacccaccgg taccatgcaa cgtatcgacc atgatggggt
    10741 tgagatcgct gcgtattcct agcagatctc gtttcccttc ctgatagcgg gtatgttgga
    10801 aattgatatt ggctcgactc ggatcagcag cttggctggt atgaacgact aaacccggta
    10861 ataataaagc agccgaagtt agcgttttaa gggttttact cactcgataa atggagtcgt
    10921 tattagtaga aaagctaaac taatctctct ttagaaacac ccgcacccac cgccacctcc
    10981 tgctgaattc gtgcttgcag cagattcacg gcttccataa ttatgctggc gaatcccgct
    11041 ttggagtggt tgaggatcta atgccatatg cggtttagca agaataccgc gctcccatgg
    11101 cgcgacattg acgcaaccag ccaataagaa actaatgaac agaaacaaaa taaatgttga
    11161 tcgtctcatt cgactagaaa ttgcagcaac gatgaagctt aattgacctt gcctaaggta
    11221 acattctcag caagtaattg ttcgatcaga tgacgcagct cttgcatttc gccagcacgg
    11281 aaaccgcggt ggatatggcg cactaagcca ttcttgtcaa tgatataagt ggaaggcatg
    11341 gcaatgacgc caaaatcctc tgcacatcgc ttcgtttcat ccatcaatac cgagaaatta
    11401 actggatgtt gtttgaggaa atttatagcg tcctgggaat tttcatccaa attgacgcca
    11461 atgacatgta gccctttttc tttaaactca tccattaact gtgtcatgaa aggaaacgat
    11521 tgaatacaag ggggacacca ggaagcccaa aaatcaacgt aaaccacttc cccttttaaa
    11581 gtactccaat cttcaacttt ttttccatct aatgcagtca atttacattg cggtggcgga
    11641 acatcgcctt caagatgtgc aaaactcggc gatacagcaa aacaagttaa agtaaccgca
    11701 agcgttaatc catagaactt gttggatatt ttcattgtta ttattcgcta ttaaaaccaa
    11761 tgtgacatta tgcggtaatc tatcgcgcta ttcaaactga tagacaccga tatccgttcc
    11821 cgtatgcaca tcattggcag tcgtacagtg gtattcaatc gagaaatcac gtgcacctgg
    11881 ggctgttttg ttgactagaa tcagataaac cccattcccc cctttgacgc taatcgatgg
    11941 actaccattt ccgtcacccg acacaggatc gctcacacta gcggcacgat ttcctttcaa
    12001 gatttgcaga ttaaccaata accctggaac gggaggagag tgatctctaa cgctagcaac
    12061 tagcttatct gcgggaccat taccatcatc aaaacaggtg acgaatccat aaccggtaaa
    12121 cgtcgcacta ttgccaactg aatccatggt tgccgccgca tcatgtgcta atcctgtatg
    12181 aataaccccc aacattccac ctgaaagagc taaaacggta atacattgct tgactagctt
    12241 ccttacatta aacactgtag attgtttcat gacgatcatc cttctatcat tcgatccaga
    12301 aagtataact catcagggtt atactttctg ttcctttagg tcaattaatt ggttattgcg
    12361 gccatacctg ttgcccatta actttaaccg gcatatcgcg attaatttga gctgcagaag
    12421 gtttaatctc aacatctacc ccctctccgc aggattcagg cagtggattt ttcgttaaat
    12481 ctcggctaat cgtccatggc gcatccgcat ttgcatccgc actatcaaaa ggagtaccta
    12541 aattgttagg cgtccacaaa tctacaaccc cttctgtacc aaattgatct gtaccagtta
    12601 cttggcacac aaccgcaatt gaaatataag cttttacact tttcgcgcaa gacgttggta
    12661 caatcgtagg tgcagtcaat ctaaatcgtg tcgctaccgt aagattactc ggtacaccag
    12721 gaccaccacc tccccagaat cccactacat tagcacctga aggatccatt ttttcatcca
    12781 ctatatcaaa ttgactccga tcaaagataa gttgcgcatt atttccataa ccttccacaa
    12841 aatcagtcag agcgcccgta tgaggctgac tattaactaa cactgtagag tcaactccat
    12901 ctggaaaaac aactgaaaac ccaattgctt tattacttcc acaggtatgt ccaaccagat
    12961 gattaattgt ccgagaccct tccgtcatgg taggtatatc caatcgtgta tgtgcaaacg
    13021 cgctcattcc cattccaacc aaggcggcag aaactgcaga aagtactaac gcccttgctt
    13081 tagtcatttg tttcattttt ttccctcatc aaatttttat cattcatttt taactctttt
    13141 attaaccatt cgcgaataag gttgtttcga ttatctgctt ttgcaccttg cttaacaatg
    13201 tgacaaattg tcgcaccctt ataaaaaaac tacacaggca atgggtgcaa ggataatcca
    13261 tgcctgtgta ggagggaggt taatctgcaa tgttaataaa acaggtgagc tgaatttcat
    13321 tctttgtgcc aaactttggt taaatttata gaataactat aattttttta gtaatttgtt
    13381 gcgaaccatt attctggcaa tgaaaaaaaa tagcaatgtg acaaattgcc gcatcgcccc
    13441 ttctgcaaag atactccagt taataatcat ttgaataagt agctcattca actagcaaac
    13501 tcacaatatt ttggtcaact ccaaaatact caagtgcgac aatttgtcgc atttaaaatt
    13561 accgcaatca actttacaat gacgctccca atctgataag caataccatc atgcaaccca
    13621 ctcaacataa ctctttctca aatttctggt tgtactgcac ggtttttttg acaggcgccg
    13681 cagtcatgat cattgagtta ctggggacac gcttaattgc ccctttttac ggggccagct
    13741 tatatgtttg ggcctcactc atttcggtaa caatgattgc actggcaatg ggttattttt
    13801 taggtgggta ctgggcagat cgtgctaagc atgctggttt agcgttgatt attggattag
    13861 cagccttact cacaatgacg attccgtggt tagtccaacc ggtgttactc agcaccgatc
    13921 acttaggatt aaaactcggt actttcatta gcacactgat cctttttacc cccagtctca
    13981 ccatgctcgg tatggttggc ccttttgctg ttaaacgcat tacagccaat ttagccggcg
    14041 ttggaacgag tactggttca atttatgcgg tcagcaccgt gggtagcgtg atcggaacac
    14101 tgtttctggg cttttattta ttcccacaga tcggctcaag agaaattatc attggtctcg
    14161 cagtcgggct ttttgtgctg gcttttatcg tcgcaatctt tgaacgacac cagattccat
    14221 tgatcaaaac actatcgttt gtagcctgtt ttttcttcat gggggtatcg atactcctgg
    14281 caaccacacc gttatccaat gagcgtgtca atcagcaaga taacgattat caaacacgct
    14341 ttgagcgtga aagcttgtat gggtgggtaa gggtcattga taatccttca ctcaacctac
    14401 gtctattaac cgccgacgcg tcaacaatcg gtgcagccag tatcagtcag ggtgagaatg
    14461 tattaacgta tcagaagatc gtcaaaatga ttccaaatct tgtgcctaac atgaaacaag
    14521 cgctgcttat tggacaaggt gcaggacata tggcaactac ccttgaacgc aaaggtattc
    14581 gtaccgacac cattgagatc gatcctgctg tcgcagaagc agcgcgtgac tactttgatt
    14641 ttattccaac ggggcaaaca ttgatcggcg atgcccgcta tgaaatccga aagcttaaag
    14701 gcccctacga tttggtcatc ctcgatgtat tcaccggagg atctgagcct gtccatctac
    14761 taacattcga aacgcttgaa cagattaaag cgctacttac ccc
//
LOCUS       DAOQLJ010000046        14507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1515_length_14507_cov_5.333103, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000046 DAOQLJ010000000
VERSION     DAOQLJ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 14507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14507
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1515_length_14507_cov_5.333103"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PK522_04915"
     CDS             <1..313
                     /locus_tag="PK522_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNC41106.1"
                     /translation="VQANPTIIEDNCFIGARSEIVEGVIVGENSVISMGVYIGQSTKI
                     YDRETGQVSYGRIPPGSVVVSGSLPSENKQYSLYCAVIVKQVDAKTRSKTGINELLRG
                     I"
     gene            395..1612
                     /gene="fabF"
                     /locus_tag="PK522_04920"
     CDS             395..1612
                     /gene="fabF"
                     /locus_tag="PK522_04920"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNC41107.1"
                     /translation="MRVVVTGMGVIAPIGTGVEQFWNAAVNGICGIRKISGFDASGQR
                     SQIAGEIIGFDPASVMSAKQIEQSDRFTQLALCAANFALKDAGDLDGYLPQRVAVSLG
                     SGMGGFSTFESSATRKFQKKSLPPFTVPRIMANSAAAWIATKYGFKGVNLTTSTACSS
                     GGNAIGVGLELIRSGKADVVIAGGAEACVLPLTMSGFEVLFALSTGFNNAPEQASRPF
                     DKGRDGFVIGEGAGIVVLEKEEHAKQRGALIHAYLSGYGCTCDAAHIVAPEIEGQVAA
                     MQAAIQDAQIQPEAVDYINAHATSTPLGDIVETKAIKQCFGSHAQKLSISATKSMIGH
                     SIGASAAIGSIATIMALKTGTIHPTINLVAPDPECDLDYTPNHAVRKPIQVGLCNAFG
                     FGGNNSTLVFTIN"
     gene            1631..1885
                     /locus_tag="PK522_04925"
     CDS             1631..1885
                     /locus_tag="PK522_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNC41108.1"
                     /translation="MTTADIEAKVIQFIASKVENVDASAVTAASQFKDLGLDSMDTVQ
                     LLFDAEEEFGVTFDGDEVKNLRTVGDIIDYINSHPPAAPQ"
     gene            complement(1927..6126)
                     /locus_tag="PK522_04930"
     CDS             complement(1927..6126)
                     /locus_tag="PK522_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC41109.1"
                     /translation="MMQQSTFFSVSLVLGIVSLAFAYYVGAILGLSIPYIGSNITLFW
                     PPAGIALAVLTLWGLALCPGILLGSLVVTLTIGELALPAALGVSLGNTLGPLAGAYIL
                     RKIAGFPTHFSNKQNIIRFLIISAGTSALTASFGTLILYLNDKILIEQLQLAWLGWWS
                     GDMIGILVFAPPFFFWSTQSIKNFLLNLTTRWEFILAISSCIGLSWIVFIGFTPLNAI
                     TSAIPYIVYLPLLWIGLRFNSMAVAIAVIVISSLATIGTSQGLGPFSEGNFYTDQFLL
                     WVFITTTSLISFMMISIQSSRKQVEQNLRDSESRLRLALEAANQGLYDLNIQTDQAIV
                     SPEYARMLGYDPNLFQETTEKWLERMHPEDREHTYQIYKDYINGVKSNYEVEFRQLTR
                     AGEWKWILSLGKVVEWDDQGHPLRMLGTHTDISDFKAKELVLQQNEEALHRTQIIAKL
                     GSWHFDLIQDVLTLSKEAFRIFGAGRQAPFSFSSFLAHVLPEDRENVNLAWQAFLKGD
                     PYDIEFRFKLNARTKWVRVRAEITTLDSNSVPIKAVGTIQDISERKQAIEEITQAKNL
                     LRTVIDASPDMISVKDKQHRFLLVNQSLATSQGLEPTAMLGKPDTDFWPDYQCYGDSA
                     RNIRGFHVDDNEAFSGKTVHNPADQALLANGELRWFDTIKIPLRNMQGHITAILGCAR
                     DITDRLHIESKYRALIEQIPAVTYTIAIDPEIHTVYVSPQLETQLGFSVEEWLANPQL
                     WLNQIHPEDRKQILSSLSVSLTTGSPYYSEYRIYKKDGSLTWVRDDAVWLKDAMGKPV
                     LLQGVMLDITHQRQAEEKLNTALEELRNSERFQRELRTLAEREQSRLGALLSAMNIGI
                     LFEDTDRRIEYINPTFTRMWNITEQDYLLNKPIQTLVERSNEIFSKPAHASKYLLNLL
                     NSDEITKLSELELNNGRILTQSSYPVNDAEHKLIGRLWIYEDITQERQTAQQLLYLAE
                     RDPLTGLHNRHRFQEELDNMIATSLRNQNQFALLYFDLDDFKYINDTFGHKAGDTVLT
                     RTSGEISSIVRQTEIFSRLGGDEFAILSPLKSGDDISALPSRIVNAIASIPIHFRDTN
                     IRLTTSVGVAIFPIHGETAEDLIAHADAAMYQAKNHGKNTWALYDPMRDSSEMMMNRL
                     TWYNRIIQALEQNLFEIHFQGVYETTQSNLEHLEILVRMRDKNKPDQLIMPGQFIPVA
                     EKNGQIVNIDRWVIKRSIELLHQNPNMPPVAINISGRTFDDPAIPNYISNLLNEYHVD
                     PKRLIIELTETAAVSDVQDAQRFIEAVHQTGCRVCLDDFGSGFSTFGYLKYLGVEVLK
                     IDGLFIRDLPNNRDNQIFVKAMVEVARGLGKITVAEFVEDAATLSMVKNLGVDLAQGY
                     YLGRPEANFPRDHHKKAVNYL"
     gene            6429..7406
                     /gene="cysK"
                     /locus_tag="PK522_04935"
     CDS             6429..7406
                     /gene="cysK"
                     /locus_tag="PK522_04935"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNC41110.1"
                     /translation="MSNWFKDNSRSIGRTPLVQLNRVTDGASATVLAKIEGRNPAYSV
                     KCRIGVAMVDDAERRGLLGPGKEIVEPTSGNTGIALAFVAAARGIPLTLTMPETMSIE
                     RRKLLVAFGAKLVLTEGSKGMNGAIAKANEIVESEAGRYVLLQQFKNAANPAIHELTT
                     GPEIWNDTNGAIDIFVAGIGTGGTMTGVSRYIKQAKGKPILSVAVEPSASPVISQRRA
                     GEPLTPGPHKIQGIGAGFIPDNLDLSLVDAVEQVSNEEAVVYARRLAREEGILSGISC
                     GAAVAAAVRQAKRAENAGKTIVVILPDSGERYLSSVLFEGMFNEQGLSA"
     gene            7406..8356
                     /locus_tag="PK522_04940"
     CDS             7406..8356
                     /locus_tag="PK522_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HNC41111.1"
                     /translation="MTSVNKVSDIQLKSTYLEIDEIVDALRDLRIASLGNRQGHLRPP
                     KLPSRKILISVAEGLNAAMFPNRLGSADLADEGVDHYVGHTLNMTLRALLVQIQHELH
                     SNSGLEALGDIDREKSVAITQAFAKRLPAVRNLLESDIVAAYEGDPAARSVDEVLACY
                     PGIMAIAYYRLAHELHLLGVPLIARMISEIAHSQTGIEIHPGAQIGPRFFIDHGTGVV
                     IGETAIIGQNVRLYQAVTLGAKRFPVDEKGALVKGIARHPIVEDDVVIYAGATILGRI
                     TIGKGSTIGGNVWLTRSVPPGSNVTQAQMRYELFEGGSGI"
     gene            8377..8790
                     /locus_tag="PK522_04945"
     CDS             8377..8790
                     /locus_tag="PK522_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNC41112.1"
                     /translation="MVVSTKSFLTLEDAKLVAAAAEAEALRNNWSVVIAIVDEGGHLI
                     YLQRLDNTQIGSVDVAIQKARSAIAFRRPTKVLEEQINQGHLRYLGLPGAIPIEGGLP
                     IVFKDQFLGAIGVSGVRSYQDAQIAQAGIDALLPQ"
     gene            complement(8794..9756)
                     /locus_tag="PK522_04950"
     CDS             complement(8794..9756)
                     /locus_tag="PK522_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HNC41113.1"
                     /translation="MMTRHIDIFNGDADGICALIQLRLAKPVESQLITGVKRDIALLQ
                     KVEASSGDQITVLDISLDRNRSDLDRLISVGAHITYFDHHYAGQIPQSSYLNTVIDES
                     PDTCTSLLVDRYLNGQYSLWAITAAFGDNLVQVAEQRCIMAHLNSIDTQKLRQLGELI
                     NYNGYGTQIDDLHFHPAKLYQALKQFQDPREAYDEAPEITTLVTGYTEDMEHAKMMIA
                     EIATSTAAVYRLPDTAWARRAVGVWANDLSQDFPARAHLIICPDGENSYTVSVRAAKD
                     HPYGASAFCRRYPGGGGREAAAGINKLPESNLAELIQAFALAFK"
     gene            complement(9777..10883)
                     /locus_tag="PK522_04955"
     CDS             complement(9777..10883)
                     /locus_tag="PK522_04955"
                     /inference="COORDINATES: protein motif:HMM:NF020874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HNC41114.1"
                     /translation="MIRLAILGTRGIPARYGGFETFAEEIAERLQQKGLEVTVYCEKG
                     CNNPVIEIYKGVRLVYVPSPKLGPFSTILFDLFCLIVARNRHDVIYMLGYGSSLFCFI
                     PRLWGKQVWLNMDGIEWKRTKWSFPARLWLRTMEWIAMLTPNRLIADAYAIQDHIKKH
                     YPYSPPISVIPYGAPEVLQADSSPLAEWDLATDRYYLLVCRLEPENHVLEILQGYTAS
                     NSNLPLVVLGNHRTGTHYVKQLLAITDARIRMLGTVYDSAKLQALRFYARAYFHGHSV
                     GGTNPSLLEALGCGNAVIAHNNVFNREVADNAALYFSTATEIPNLVEKIDTDETLRAA
                     MRLNARNIVSQRYTWKLVTDAYMALLTAPSSTEW"
     gene            complement(10895..11917)
                     /locus_tag="PK522_04960"
     CDS             complement(10895..11917)
                     /locus_tag="PK522_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNC41115.1"
                     /translation="MINFLRSYFIVLLLIAFVPVDGAFAVASTDKSLKILHFVLKDIS
                     FDKAHEIVDEAKNAGFSTIQVLLTDGLILDKAPWKTSKNAWTKDKFLSWITYIRMQGL
                     DFIPEIKLLTHQEKFFQDRYPNLMFNKSTYDPRNESTYLIVFSLIDEILEAINPKAIH
                     IGHDELAGFSLDTEKKWLNPNEEILPASLFLQHINRIHNYLKQKKVATWIWGDMLISP
                     NEFPNMRKKDLHGNLSGYAKELRNQLPRDIVICDWHYADEQPNFPTLSKLQEEGFKVI
                     GVTWKNQKTIRNFSRYAKNHNAYGMMATTWFHVQRNEMELVKWIIQTSGLLFQNPDAT
                     VLPQPK"
     gene            complement(11923..12930)
                     /locus_tag="PK522_04965"
     CDS             complement(11923..12930)
                     /locus_tag="PK522_04965"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNC41116.1"
                     /translation="MIAQVDSTYIRIRPWKLWSRLVSYVLFEGRPITTRGQWINPLLF
                     AHFAIEKKLPLIAKKVEKPVFILGTGRSGTTILGVILSMHRHVGFLNEPKAIWHSIYP
                     NEDLIGNYSLNSARYRLEAKDASSEVIQSAHRIFGTYLATTFSERLVDKYPELIFRIP
                     FIRTIFPNAKFLFITRNGWDTCSSISNWSNRLGLQAKSEIHDWWGRNRRKWNLLINEI
                     VLEHNDLASLAPQIYDWSNQSDMAAVEWIVTMREGLKLQEQFPNDILQVRYETLCLEP
                     TKILSEILKFTELSVDDAKFFSYAQFTLQPSSPKNPFELNKLIEEPFYEVMHKLGYKS
                     E"
     gene            complement(12932..13945)
                     /locus_tag="PK522_04970"
     CDS             complement(12932..13945)
                     /locus_tag="PK522_04970"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNC41117.1"
                     /translation="MTEMIFIVGNSRSGTTMLGRIFGNHSKVYTFGELHFFENQVEAK
                     DIYTPPIWEEKKCISLLNRLITTARDGFFQSPIPGNYSSDITEILSTSNFRDPISVYS
                     AFLQYETELNKKSIPCEQTPRYLFFSSEILNKFPEAKVINIVRDPRDVLLSQKHKWRR
                     KFLGAKNIPILESIRAWVNYHPYTISLLWLGALRKAEEMETHPRFTTIRFEDLLNKPE
                     TTVRSLCKFTDLAFESEMLNVPQIGSSTGNDTLLKGINSRHAGKWRNSDLSPTEVSIC
                     QRVTNTQMRRMGYDLVKTDSPFFLRTASKLTFILKSSIALLLNLNRTKNLRETLLRRL
                     TTK"
     gene            complement(13942..>14507)
                     /locus_tag="PK522_04975"
     CDS             complement(13942..>14507)
                     /locus_tag="PK522_04975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC41118.1"
                     /translation="TLVPPDQPGKYFLFVGRISEEKGINVLLDAYKLLGQSAPSLKIV
                     GSHNHPSHLDNKIKYLGLEKKVEWIGFKTGKELTQLYKGCLALINPSQLNETFGLTCL
                     EALAQGRPVIASRVGAFPEIITHNENGLLVKSGSPMALAEAMTQLLENPYQAQAMGLC
                     GLEKVNQLFSKVAHYQKLCEVYRKVTI"
BASE COUNT         4203 a         2784 c         3140 g         4380 t
ORIGIN      
        1 ggtacaagca aacccgacca tcattgaaga taattgcttt attggtgccc gatctgaaat
       61 tgtcgaaggt gttatcgttg gtgagaattc cgtgatctca atgggggttt atattgggca
      121 aagtactaaa atctatgacc gggaaacggg gcaagttagc tatgggcgta ttccacctgg
      181 atcagtcgtc gtgtcaggaa gtttaccttc agagaataag caatatagtc tctattgtgc
      241 ggtcattgta aaacaagtcg atgccaagac tcgctctaag actggaatta atgagttgct
      301 acgtggaatt taacgactcg tcggacttct acttaacgtt agcgaataac agaatgtttt
      361 gagcaatcga ttttcaatta ctggatgatt agaaatgcgt gtcgttgtga ctggaatggg
      421 ggttattgct ccgatcggga caggagttga acaattttgg aatgctgcag taaatggaat
      481 ctgtgggata cgcaaaatta gtggctttga tgcatctggc caacgttccc aaattgccgg
      541 tgaaatcatt gggtttgacc ctgcttccgt gatgtcagcc aaacaaattg aacaatctga
      601 tcgatttaca cagctagcgt tatgtgccgc taattttgcg cttaaagatg cgggagatct
      661 cgatggttat ttacctcagc gcgtagcagt gagcttaggt tcggggatgg gtgggttttc
      721 aacgtttgaa tcttcagcga cacgaaaatt tcagaaaaaa tcattacccc cttttacggt
      781 tccacggatc atggccaatt ctgcagcagc ctggattgcg accaagtacg gatttaaagg
      841 tgttaacctg acgacgagta cagcgtgctc atcaggcggt aatgcgattg gggtggggct
      901 tgaattgatt cgctctggaa aagcagatgt tgttatcgca ggaggtgctg aagcatgcgt
      961 attacctttg acgatgtctg gttttgaagt gttatttgcg ttgtctactg gatttaataa
     1021 tgccccagaa caggcatcaa gaccctttga caaaggtcga gatggctttg taattggtga
     1081 aggcgcaggt atcgttgttc tagaaaaaga agaacatgcc aaacaacgtg gagccttaat
     1141 tcatgcatat ctatccggtt atggttgtac ttgcgatgca gcacatatcg tggcgccgga
     1201 aatagagggt caagttgccg cgatgcaagc agccatacaa gatgcacaga tccaacctga
     1261 agcggttgat tatattaatg cgcatgcaac ttctacacca ctcggcgata tcgtagagac
     1321 taaagctatt aaacagtgct ttggatccca tgcgcagaaa ttatccatta gtgcgactaa
     1381 atcaatgatt ggacactcta tcggtgcgtc ggcagcaatt gggagcattg ccacgatcat
     1441 ggcactaaaa actggcacta ttcatcccac gatcaattta gtggcgcccg atccagaatg
     1501 cgatcttgac tatacaccca atcacgcagt tagaaagccg atccaggttg ggttatgtaa
     1561 cgcttttggc tttggtggta ataattccac acttgttttt acaataaatt aaatcatagg
     1621 aatacatcat atgactacag cagatatcga agcaaaagtc attcagttca ttgcttctaa
     1681 agttgagaat gtcgatgctt cagcggttac agcagcatca caatttaaag atttaggttt
     1741 ggactcaatg gatacagttc agctattatt tgatgcggaa gaagaatttg gtgtcacatt
     1801 tgatggcgat gaagtgaaga atctccgaac ggtaggggat attatcgatt atattaacag
     1861 ccatcctcca gcagcacctc aataaagctg agtgggtgga ttgctaaaca tggtttagta
     1921 aattatttag agataattga ccgctttttt atgatgatct ctagggaaat tggcttctgg
     1981 gcgacctaag taataaccct gtgcaaggtc aaccccaaga ttcttgacca tgctcaaggt
     2041 tgcagcatct tcaacaaatt cagccacagt gattttacct aatccacgtg ctacctccac
     2101 catggctttg acaaatattt gattatcacg attattcgga agatcacgaa taaataagcc
     2161 atcgattttt aatacttcca caccaagata tttgagataa ccaaaggtag aaaacccact
     2221 cccaaaatca tccaagcaaa cacggcaacc tgtttgatga actgcctcaa tgaaacgttg
     2281 tgcatcttgg acgtcagaaa ctgcagccgt ttctgttaac tcgataatca aacgtttggg
     2341 atcgacatga tattcattta atagattact aatgtaattc ggtatagcag gatcatcaaa
     2401 ggttcgacca gaaatattga ttgcaacggg cggcatattg gggttttggt ggagcagctc
     2461 aatactacgt tttattaccc aacgatcaat attgacgatt tgcccatttt tctcagcaac
     2521 tgggataaat tgtcccggca taatgagctg atcaggttta tttttatcgc gcattctaac
     2581 gagaatttct aggtgttcta aattactctg ggttgtttcg taaacacctt gaaaatgaat
     2641 ttcaaacaag ttttgttcaa gcgcttgtat gatccggtta taccacgtta accgattcat
     2701 catcatttct gacgaatcac gcattggatc atagagtgcc caggtgtttt ttccatgatt
     2761 tttagcctga tacattgcgg catctgcgtg ggcaatcaag tcttcagccg tttctccatg
     2821 gatgggaaag attgcgactc caacgctggt ggtgaggcgt atattcgtat cacgaaaatg
     2881 aattgggata gaagcaatag cattgacgat gcgagaaggt aatgcgctga tatcatcgcc
     2941 tgattttagt ggactcaaaa tcgcgaattc atcccccccg agtcgtgaaa aaatttccgt
     3001 ttgtcgaacg atgctagaaa tttccccaga cgtacgagtt aaaacggtat ctccggcttt
     3061 atggccaaat gtatcattga tatatttaaa atcatcgaga tcgaaataaa gtagtgcaaa
     3121 ttgattctga ttgcgtaatg aggtggcaat catattatca agttcttctt ggaagcgatg
     3181 acgattatgt aatccagtca gcgggtctcg ttccgcgaga tacagaagtt gttgggcagt
     3241 ttgtctttct tgcgtgatat cttcataaat ccagaggcgt ccgatgagtt tatgttctgc
     3301 atcattgact ggataggatg actgagtcag gattctacca ttattcagtt ctaattctga
     3361 taacttagta atttcatctg aattaagtag gtttaataaa tattttgagg catgcgccgg
     3421 ttttgagaat atctcattag atcgttctac aagcgtttga atgggtttat tcaataaata
     3481 gtcctgttca gtgatgttcc acattcgcgt gaaagtagga ttgatgtatt cgattcgtcg
     3541 atcggtgtct tcaaataaaa taccgatatt catcgcagaa agtaaagcac ctaatcgact
     3601 ctgttcacgc tcggctaacg ttcgtaattc gcgctgaaat ctttcggaat tacgcaattc
     3661 ttccaaagcc gtatttaatt tttcctcggc ttgccgctgg tgtgtgatgt caagcatgac
     3721 accttggagt agcacaggct tacccatcgc atctttcaac caaactgcat catctcttac
     3781 ccaagtgaga ctgccatctt ttttgtagat acgatattct gaataataag gcgatcccgt
     3841 tgttaaactg accgataagc ttgataatat ttgcttgcga tcttcagggt gaatttgatt
     3901 taaccatagt tgtggattcg ctaaccattc ctcgacacta aaccctagtt gggtttcaag
     3961 ctgtggactg acatagacag tatgtatttc tggatcaata gcgattgtgt aggttactgc
     4021 tggaatctgc tcaatcaagg cgcggtattt tgattcgata tggagtcgat ccgtaatatc
     4081 acgagcacaa cctaatatcg cggtaatatg tccttgcata ttacgcagcg ggattttaat
     4141 cgtatcgaac cagcggagtt cgccattggc taatagtgct tggtctgctg gattatgcac
     4201 agttttgcct gagaaagctt cgttatcatc tacatgaaat ccccgaatgt ttcgggctga
     4261 atcaccataa cattgatagt caggccaaaa atctgtatcg ggtttgccta gcattgctgt
     4321 tggttctaac ccttggctgg tagctaatga ctgattcaca agtagaaaac gatgctgctt
     4381 gtcttttaca gaaatcatat cgggagaagc atcaatgacc gtgcggagca aattcttggc
     4441 ttgagtgatt tcttcaattg cttgtttacg ttcgctgata tcttgaatgg ttccaacggc
     4501 tttgatagga acactattac tatccagtgt cgtaatttcg gctctgactc tgacccattt
     4561 tgtccgtgca ttgagtttaa aacggaactc aatatcgtaa gggtctcctt ttaggaacgc
     4621 ttgccaagcc agattaacgt tttctcgatc ctctgggagt acatgcgcta aaaaactact
     4681 aaagctgaaa ggtgcctgcc ttccggcacc gaatatccga aaggcttcct tagacaaggt
     4741 aaggacatcc tgtatcagat cgaaatgcca gcttcctaat tttgcgatga tttgtgtgcg
     4801 atgcagcgcc tcttcatttt gttgaagcac cagttctttt gctttaaagt cactaatatc
     4861 ggtatgcgtg cctagcatcc gcagtggatg cccttgatcg tcccattcta ctaccttgcc
     4921 tagcgataga atccatttcc attccccagc tcgggtaagt tggcggaatt ccacttcata
     4981 attgcttttt acgccattaa tataatcttt ataaatttga taggtatgct cgcgatcctc
     5041 tgggtgcatc cgttcaagcc atttttcggt ggtttcctga aataaattgg gatcataacc
     5101 aagcatacgt gcatattctg gactgacgat cgcttgatcg gtttgaatat ttaagtcata
     5161 caacccttga ttagctgctt caagtgctaa tctaaggcgc gattcactat cccgcaaatt
     5221 ttgttcaacc tgttttcgtg atgattgaat gcttatcatc atgaatgaga tgagcgaagt
     5281 ggttgtgata aatacccata acaggaattg atcggtatag aaatttcctt ctgagaaggg
     5341 tccaagccct tgcgaggtgc ctatggtggc taatgatgag ataacgataa ctgctattgc
     5401 cactgccatt gaattaaagc gaagcccaat ccacaataaa ggaaggtata caatataagg
     5461 gatagccgat gtgatcgcat taagtggggt aaatccgatg aaaacgatcc aagataatcc
     5521 tatacaacta ctaattgcta aaatgaattc ccatctagtt gttaagttga gtaggaagtt
     5581 tttgatggat tgagttgacc aaaaaaagaa tgggggagca aaaactagga taccaatcat
     5641 atccccagac caccacccta accatgctaa ctgaagttgc tcaatgagta tcttgtcatt
     5701 gagatagagt atcagcgtgc caaaactagc tgtgagcgct gaagttccag cgctgataat
     5761 aagaaagcga ataatatttt gtttattaga aaagtgagta ggaaaacctg ctatttttcg
     5821 aagaatataa gcaccggcca atggacccag tgtattacct aatgaaacac ctaaagcggc
     5881 aggcaaagct aattcaccaa tcgttagcgt gacaaccaat gagccgagaa ggataccggg
     5941 gcaaagcgct aatccccata gtgttaatac tgccaaagca attccagcag gtggccaaaa
     6001 tagggtgata tttgagccaa tgtacggtat gctcaagccc agtattgctc caacgtaata
     6061 agcgaatgct aaggaaacta ttcccagaac caatgaaacc gagaaaaagg ttgattgttg
     6121 catcatgaat ggttaagcat attcggtaaa gaaaatctac tgagttggct aaatagggtt
     6181 ttgactagct gcattttcat cgatagttgg atgagcatag cattcaatta gtgctatgtt
     6241 taactttaaa atagatgggt ttcatatcac tattctaatt tttatttaat aaaaacttta
     6301 aaactagcct atcaggtaga tcaataattt agctaagatt taggaacgag taaaatagag
     6361 tatcaaatct aattcattct gagtttcaat ataaaattgt cagaagactt atatgaaagg
     6421 ggtagcacat gtcaaattgg tttaaggata actcaaggtc aattggccga acaccacttg
     6481 ttcaactgaa tcgagtcact gatggtgcat cggcaacggt actcgcaaaa attgaggggc
     6541 gtaacccagc ctattctgta aagtgtcgaa tcggggtggc aatggttgat gatgcagaac
     6601 ggagggggtt gttgggtcct ggaaaagaaa tcgtcgaacc aaccagcgga aatacaggca
     6661 tcgctttagc atttgttgct gctgcaagag ggattccgct tacgttaacg atgccagaaa
     6721 cgatgagtat tgagcgtcgt aaattattag ttgcctttgg agcaaaactg gtactcactg
     6781 agggttcaaa ggggatgaat ggtgcaattg caaaagcaaa tgaaatcgtg gaatcagaag
     6841 caggtcgata tgttctgcta caacaattta agaatgctgc aaatcctgcg atacacgagc
     6901 tgacaacggg acctgaaatt tggaatgata ccaatggcgc aattgatatt tttgttgcag
     6961 gtattggaac agggggaaca atgaccggtg tatcgcgata tatcaaacag gctaaaggta
     7021 agcctattct atcagtagca gttgagcctt cagcaagccc agtgatttct cagcgacgtg
     7081 caggtgaacc cttaacacct gggccacata aaattcaagg aatcggggca gggtttattc
     7141 ccgataatct tgatctttct ctggtggatg cggtggagca ggtgtcaaat gaagaagcag
     7201 ttgtatatgc gcgtcgatta gctcgggaag aggggattct ttctggaatc tcttgtggcg
     7261 cagctgtagc tgcagcggtt aggcaagcga aacgagctga aaatgcgggc aaaacaatcg
     7321 tagtgatcct gcctgactcg ggagaacggt atttgagtag cgttcttttt gaaggcatgt
     7381 ttaatgaaca aggactaagc gcatagtgac tagtgttaat aaggttagcg atattcagct
     7441 taaaagcact tacttggaaa tcgatgagat tgtcgatgcg ctgagggatt taaggattgc
     7501 ctcactgggg aatcgacaag gtcatctacg tcctcctaaa ttaccatccc gcaagattct
     7561 tattagtgtc gctgagggac tcaatgcggc catgttcccc aatcgtttgg gttcagcaga
     7621 tttagcggat gagggggttg atcattatgt cgggcatacg ctcaatatga ctttgcgcgc
     7681 actgctggta caaattcagc atgagcttca ctctaactct ggacttgagg cgctcggtga
     7741 tatcgatcgt gaaaaatcag tcgcaattac acaggccttt gctaaacgat tgcctgccgt
     7801 gaggaattta ttggaaagtg atattgtggc ggcttatgaa ggtgatccgg ccgcacgaag
     7861 tgttgatgaa gtgctggctt gctaccctgg gattatggcc attgcctatt atcgattggc
     7921 ccatgaatta catctgcttg gggttccact cattgcgcgt atgatttctg aaattgcgca
     7981 ttcgcaaact gggattgaaa ttcatccagg tgctcaaata ggcccaagat tctttatcga
     8041 ccatggaacc ggagtggtaa tcggtgaaac ggcgatcatt ggccaaaatg tgcgtcttta
     8101 tcaagcggtc actttagggg ctaagcgatt tccggttgat gagaaaggtg ctttagtcaa
     8161 aggcattgcg cgccacccga ttgttgaaga tgatgtcgta atctatgcag gtgcgacaat
     8221 cttggggcgt atcacgattg gtaagggttc tactatcggt gggaatgttt ggttgacccg
     8281 tagtgttcca ccggggagta atgttactca agcacaaatg cgctatgagt tatttgaagg
     8341 gggaagtgga atttaatatc atttttctga ggagaaattg tggttagtac caaaagtttt
     8401 ttgacgctgg aagatgcaaa gcttgttgca gctgcagctg aagcggaggc attacgcaat
     8461 aattggtcag ttgttatcgc aatagtcgat gaaggtgggc atttaatata cttgcagcga
     8521 cttgataata ctcaaattgg gagtgttgat gttgccatcc aaaaagcacg ttcggcgatt
     8581 gcttttagac ggccaactaa agtactcgaa gaacagatca atcaagggca cttgcgatac
     8641 ctagggctac cgggagcaat accgattgag ggcggattac ctattgtgtt taaagatcaa
     8701 ttcttgggtg ctattggtgt tagtggtgtt cggtcatacc aagatgcgca gattgcacaa
     8761 gctggtatcg atgctttatt acctcagtaa aacttacttg aaagctaacg cgaacgcttg
     8821 aattaactct gcgagattgg attcagggag tttattaatt ccggctgcgg cttctcgtcc
     8881 acccccacca ggatagcgtc tacagaatgc cgatgcgcca taaggatgat cttttgctgc
     8941 acggacacta actgtgtatg aattttcgcc atcaggacag atgatcaaat gggcgcgagc
     9001 aggaaaatcc tgtgaaaggt cattggccca aacccctacc gcacgacggg cccaagctgt
     9061 atcaggtaat cgataaacgg ctgctgtgga tgtggcaatt tcggcaatca tcattttggc
     9121 gtgttccata tcttctgtat aaccagtaac caaagtagtg atttccggtg cttcatcgta
     9181 agcttctctg ggatcttgga attgctttag tgcttggtaa agcttggcag gatgaaaatg
     9241 caaatcatcg atctgcgttc catatccatt gtagttgatc aactcaccca attggcgcag
     9301 tttttgtgta tcaattgaat taagatgcgc cataatgcag cgttgctcag caacctggac
     9361 taggttatcg ccgaacgcag cggtaatcgc ccataatgaa tactgcccat tgagataacg
     9421 atcaacgagt agactggtgc aagtatccgg ggattcatca atcactgtat ttaaatagct
     9481 gctttgaggt atctgccccg catagtgatg atcaaaatag gtaatatgtg cccccacaga
     9541 aattaatcga tccaaatcac tgcggttacg atccagtgag atatcaagca ctgtgatttg
     9601 atctcctgag ctagcttcaa ctttctggag taaagcaata tcgcgtttaa cgcccgttat
     9661 gagctgtgat tcgacaggct tggctaagcg caattgtatt aacgcgcaaa taccatctgc
     9721 gtctccattg aagatatcaa tatggcgagt catcatgatt tttctttaag caattatcac
     9781 cattcagtcg aggatggtgc tgtaagtaac gccatataag cgtcggtgac tagtttccag
     9841 gtatatctct gggatacaat gtttcgagca tttaagcgca ttgctgcacg aagtgtttcg
     9901 tcagtatcaa ttttttctac tagattaggt atctctgttg ctgtagaaaa ataaagtgct
     9961 gcattgtcag cgacttcgcg attaaaaaca ttattgtgtg cgataacggc attaccgcaa
    10021 cccaatgctt cgagtaacga tgggttagtt ccaccaacag aatggccgtg aaagtaagcg
    10081 cgggcataaa aacgtaatgc ttgtaattta gccgaatcat aaactgtgcc tagcatccga
    10141 attcgggcat cggtgatggc tagcagttgt ttcacataat gagtcccagt ccgatgattg
    10201 ccaagtacaa ctagtggtaa gttactattt gatgctgtat acccttgcag tatttctaat
    10261 acatggtttt caggctctaa tcgacatact agtaaatagt agcgatctgt agctaaatcc
    10321 cactctgcta gcggtgatga atcagcttgt aggacttcgg gtgcaccata aggaataact
    10381 gaaatcggtg gagaataagg ataatgtttt tttatatgat cttgaatagc ataagcatca
    10441 gcaataagcc gattaggagt tagcattgca atccactcca tagtacgtaa ccagagtcgc
    10501 gcaggaaacg accatttagt tcgtttccat tcaataccat ccatatttaa ccagacttgt
    10561 tttccccata acctgggaat aaagcaaaat aaactagagc catagcccaa catataaatg
    10621 acatcatgtc gatttcgtgc aacgatcaag cagaataaat cgaataaaat ggtactaaat
    10681 gggcctaatt tgggtgaagg tacataaact aagcgaactc ctttatatat ctcaattaca
    10741 ggattattac atcctttctc acaataaaca gttacctcta gtcctttttg ttgaagccgc
    10801 tcggcaattt cttctgcaaa cgtttcaaaa ccaccataac gagccgggat tccacgtgtc
    10861 cctagaattg caagacgtat caaattgtta ccttttattt gggttgcggt aataccgttg
    10921 cgtcaggatt ttggaatagt aatcccgacg tttggataat ccattttacg agttccattt
    10981 cgttacgttg tacatgaaac caggtggttg ccatcattcc gtaagcatta tgattttttg
    11041 catagcgact gaagttgcgt atggtttttt gatttttcca agtaactcct atcactttaa
    11101 atccttcttc ttgcagttta ctgagtgtgg gaaaattagg ttgctcatct gcgtaatgcc
    11161 aatcacaaat tacaatatct ctcggtaatt gatttcgtaa ttctttagcg tatccagaaa
    11221 gatttccatg caaatctttt ttacgcatat taggaaattc attggggcta attaacatat
    11281 caccccatat ccaagttgct acttttttct gctttaaata attatgaata cgattaatat
    11341 gttgtaaaaa taaagaagct gggagtattt cttcattagg gttcaaccat tttttctcag
    11401 tatctaagct aaatcctgct aattcatcat gaccaatgtg aatagctttg ggattaattg
    11461 cttctagaat ttcatcaatt aaagaaaata caattaaata ggttgattca ttacgagggt
    11521 cataagtgga tttattgaac attaaattag ggtagcgatc ctggaaaaat ttttcctggt
    11581 gcgttagtaa tttaatttct ggaatgaaat ctaatccttg catgcggata tatgttatcc
    11641 atgaaagaaa tttatctttt gtccatgcgt tttttgatgt tttccaagga gctttgtcta
    11701 gtattaatcc atcagttaat agaacttgaa ttgtactgaa accagcattt tttgcttcat
    11761 caacaatttc atgagctttg tcaaatgaga tatcttttaa aacaaagtga aggattttga
    11821 gtgatttatc agtggacgct acagcaaaag ctccatcaac tggaacaaat gctatcagta
    11881 gcaatactat aaaatagcta cgcaaaaaat taatcataaa aactactcac ttttatatcc
    11941 caatttatgc attacttcat aaaaaggttc ttcaatcagc ttatttaatt caaatggatt
    12001 cttcggacta gaaggctgta aagtgaattg agcatatgaa aagaattttg catcatctac
    12061 tgatagctca gtaaacttta atatctcact tagaatttta gtaggttcca agcagagtgt
    12121 ttcataccga acttgtaaaa tatcatttgg aaattgttct tgcagcttaa gaccttctct
    12181 cattgtgaca atccattcta ctgcagccat gtcggattga ttagaccagt catagatttg
    12241 aggtgctaat gaagctaaat cattgtgttc taaaacaatc tcgtttatta ataaattcca
    12301 ttttcgtcta tttcttcccc accaatcatg tatttcagat tttgcctgta gacctagtcg
    12361 attagaccag ttactaattg agctacaagt atcccaacca tttctggtta taaaaagaaa
    12421 ttttgcattt ggaaaaatgg ttctgataaa tggaatacga aagattagct caggatattt
    12481 atctacaagt ctttcagaaa aagtagtagc caaataggta ccaaaaattc gatgtgcaga
    12541 ttgaataact tctgaggaag catctttagc ctctagtcta tagcgagcac tatttaaact
    12601 ataatttcct attaaatctt cattggggta tatagagtgc catatagctt tgggttcgtt
    12661 aagaaaacca acatgccgat gcatggaaag aataactcca agtatggtag tgccactacg
    12721 acctgtacct agaataaaca ctggtttttc gactttcttg gctattaatg gtagtttttt
    12781 ttcaatggcg aaatgtgcga agagcaacgg attaatccat tgcccgcgag tagttattgg
    12841 tcgtccttca aacaatacat aactaactag tcgactccat aatttccagg ggcgtattct
    12901 gatataagta gaatcaactt gtgcaatcat tttattttgt ggttaagcga cggagtagtg
    12961 tttcccgaag gttttttgtg cggtttaagt taagtaacag tgctatagat gatttaagta
    13021 tgaaagtcag cttgcttgca gtccgaagaa aaaagggtga atcagtttta actagatcat
    13081 agcccattct tctcatttga gtatttgtta cacgctgaca tatagatact tcggtaggtg
    13141 aaagatcaga attacgccat ttgccagcat gtctactatt tattcccttg agcaatgtat
    13201 cattaccagt tgatgaacct atttgcggta cgtttagcat ttcagattca aatgctaaat
    13261 cagtaaattt acatagtgaa cgaactgtag tttctggttt gtttaataag tcttcaaatc
    13321 gaattgttgt aaatcttgga tgtgtttcca tttcctccgc ttttcttaag gcaccaagcc
    13381 aaagtaatga aatagtataa gggtgataat ttacccatgc acggatagat tctaatattg
    13441 gaatattttt tgctccaagg aattttctac gccatttatg tttttgagat aataaaacat
    13501 ctcgaggatc acgtacgata ttgatgactt tagcttccgg aaatttattt agaatctcag
    13561 atgaaaaaaa aaggtaacga ggggtttgct cacagggaat cgattttttg ttaagttctg
    13621 tttcatactg aagaaatgct gaataaactg aaatgggatc acggaaattt gatgttgaaa
    13681 gaatttctgt gatgtctgaa ctataatttc ctggtattgg actctgaaaa aatccatccc
    13741 gcgctgttgt aattaagcga ttcaacaagc ttatacattt tttctcttcc caaattggtg
    13801 gagtataaat atctttagct tcaacttgat tttcaaaaaa atgtaattca ccaaaggtat
    13861 atactttgga atgattacca aatatcctac ctagcatcgt tgttccactg cggctatttc
    13921 caacgataaa aatcatttca gtcatatagt tactttccta taaacctcac aaagcttttg
    13981 gtagtgagct actttagaga ataactgatt aactttttct aaaccacaga gtcccatagc
    14041 ttgagcctga taaggatttt ctaaaagctg agtcattgct tctgctagag ccattggtga
    14101 tcccgacttg accaataaac cattttcatt atgagttata atttctggga aagctcctac
    14161 ccgtgatgcg attacaggtc ttccttgcgc taaagcttcc agacaagtta atccaaatgt
    14221 ttcattgagc tgtgaagggt taattagggc caagcatcct ttatataatt gtgttagttc
    14281 tttacccgtt ttgaatccaa tccattcgac ttttttttct agacccaaat attttatttt
    14341 gttatctaag tgtgatgggt gattatgact acctactatt ttgagcgatg gagctgattg
    14401 acctaggagt ttataagcgt ccaatagtac attaatgcct ttctcttctg aaatacgtcc
    14461 aacaaataaa aaatattttc caggctgatc tggtggaact aatgttg
//
LOCUS       DAOQLJ010000047        14326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1548_length_14326_cov_6.497442, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000047 DAOQLJ010000000
VERSION     DAOQLJ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 14326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14326
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1548_length_14326_cov_6.497442"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="PK522_04980"
     CDS             complement(<1..1036)
                     /locus_tag="PK522_04980"
                     /inference="COORDINATES: protein motif:HMM:NF040115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41119.1"
                     /translation="MSIQGFTPAQIAAFTTSQIQSLTTDDFKKLSTAQINALTSEQIG
                     AIETEDLSVLSATQIKSFDAQEFADGMLTYQIEALNQTHIAALSTDQLSVLTTDQIQA
                     IETRDLIKLSAAQLNALSTEQFNNLSSDQIQSLTATQIKGLSTDQLSTLTTDNVQLLT
                     GAQIASLSITQLNSFDSDQIAAIDVADVAAFTATQVKGMDSSQIAALTTSQITQLSNN
                     QLAALTTTQIANIETDDLAAMTSSQITAFSAKQIVAFTSDQLNNMTSDQIQALTATQI
                     KGLTTDQVSSLTSDNIQLLTSAQVAALSTAQLNSLDSDQIAALDTGDVAALTANQIKG
                     LGTTQIQDLTS"
     gene            1457..2587
                     /gene="flhB"
                     /locus_tag="PK522_04985"
     CDS             1457..2587
                     /gene="flhB"
                     /locus_tag="PK522_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNC41120.1"
                     /translation="MADESDLEKTEEATPRRLEKAREEGQVARSQELTTFALLMVAGG
                     ALWLLGSSFITNLMEILKRGLQIQPEALLDSTSLPVRLFQHAIDSLIASAPLFAVFVI
                     VAAIAPTLLSGLLFTTKALMPDFKRLSPIQGFKRIFSMHGFVELVKAIVKTIVVGGVT
                     ALLIWYNKEAVIGLILLPIDLGAESMGSLLLLIFMLIAAAMVLIVGIDVPFQIWQHKH
                     RLKMTKEEIRKESKEDEGDPFVKARIRSLQREAARQRMMAEVPKADVIVTNPTHYAVA
                     LKYHGESMRAPRVVAKGVHLLAQRIREIAEENRVPVLEVPPLARALYHHTELNDEIPE
                     TLYTAVAQILAYVFQLKRYQTYGGVVPQLPSDLTVPQELDHA"
     gene            2655..4745
                     /gene="flhA"
                     /locus_tag="PK522_04990"
     CDS             2655..4745
                     /gene="flhA"
                     /locus_tag="PK522_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNC41121.1"
                     /translation="MNHSLSLTSLIDKNNLQSIAGPLLIIMILAMMVLPLPPFFLDVL
                     FTFNIALSIIVLLVSLYTKDALDFVAFPTVLLITTLLRLSLNVASTRVVLMEGHSGPD
                     AAGKVIEAFGHFLVGGNFAVGIVVFIILVVINFVVITKGAGRIAEVSARFTLDAMPGK
                     QMAIDADLNAGLIGEAEAKKRRATIAQEADFYGSMDGASKFVRGDAIAGILIMVITII
                     GGLVVGVFQHDLDVSAAAKNYTILTIGDGLVAQIPSLIISTAAGMVVSRVSSDQDLGQ
                     QVVSQLFNKPQVLFLTAGILGVMGLIPGMPNFVFLLLASIIAGIAYFKVNYLPEPEAV
                     TEEATSSPVAETEGREASWNDVMPVDMLGLEVGYRLIPLVDKTQQGDLLKRINGIRKK
                     FAQEVGFLPPVVHIRDNLELRPNAYRITLKGSVIGQGESYNGMYLAINPGQATKTLSG
                     VNTSDPAFGLPAVWIEPALKDDAQAHGYTVVDPSTVVTTHVNHLMRSYAAELLGREEL
                     QKLFDHLGRSIPKLTEDLIPKLLPLATVQKILQNLLEEGVHIRDVRTIVDVLTEHATR
                     TQDTNELTALVRVALGRSILEQFFPSGEELQVMSLHHNLESILVQVAQSGLVAGAGIE
                     PGLADTLLKEVSKAVDQHEQLGIPAVLLVPDSIRLLLARFLRRPLPQLKVISHAEVVD
                     SRKIKFVSIVGGNK"
     gene            4742..6007
                     /gene="flhF"
                     /locus_tag="PK522_04995"
     CDS             4742..6007
                     /gene="flhF"
                     /locus_tag="PK522_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNC41122.1"
                     /translation="MKMRRFIAKTSHEALRNVKEELGADAVILSNKQVDEGIEIVALA
                     SNEMSSLVNSPESKFVPTPPPVEKKEEVLSVINPNIEVLEAIQQVKKESNISEALALN
                     IINEIQAMRHTLEDQLNSVAWGSLSQSKPETMKLMRTLLDAGFSPLLSRHLLEKLTKV
                     SNYEESLKQTVALLTLNLRTATSDQLIENGGVYALIGPTGVGKTTTIAKLAARAVIRH
                     GADKVALLTTDSYRIGGHEQLRIYGKLLGIPVRSIKDIDDLQLTLLELRSKHIVLIDT
                     VGMSQRDQMVSEQMALLRDCGTEIKHLLLLNATSNGGTLDEVISAYQYNGIEGCIITK
                     VDEAVGLGTALDVVIRRKLVLHYVTNGQKVPEDLHAANSRYLLHKIFKPIAENSPFSL
                     QDPEFALVMAGYDRNSAAKPKGYAEASYE"
     gene            6000..6887
                     /locus_tag="PK522_05000"
     CDS             6000..6887
                     /locus_tag="PK522_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC41123.1"
                     /translation="MSRKMIDQASGLRELAAMQARDSVKVFTVTGGKMGVGKTSIVVN
                     LAAALAKIGNRILILDENQHHNNVSANLGVKSHYDLTHLIKREQSLDQILAKAPDDIL
                     VLTAARGIHSLTQLSAEEQEWIIKCLCELPIDVVLVDTMIGEMGHVLPLSLASQQVLI
                     TVSGSSKSITDAYTLIKIMSQEYARQDFLVIVNKVKSELDAQSIYENIAKVAKQHLSV
                     RLEYLGCILFDEKLHRASQLCRSVVDAFPASTAAIEFKRLAEKLLRCSYSNQYSGGVD
                     NFMYRLIRTSHLSKVKLSV"
     gene            6897..7607
                     /locus_tag="PK522_05005"
     CDS             6897..7607
                     /locus_tag="PK522_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HNC41124.1"
                     /translation="MYTTSGIIDKEQFVVEFAPLVKRIAHHMMMKLPASVEVDDLIQA
                     GMIGLLDAINRYEGAYGRQFESYAAQRIRGSILDELRESDWLPRSLRKKMRQIEVAMR
                     SLEQKLGCPPSEQEIAKELNISLEEYHEALQDARGGQLIYYEDFQDDDEDHFLDRLCS
                     DSGNEPLEILVDKNLRELLVAAIDKLPIREKEVMGMHYEQEMNLREIGEVLGVSESRV
                     CQIHSQAIARLRAKLRNQ"
     gene            complement(7727..7948)
                     /locus_tag="PK522_05010"
     CDS             complement(7727..7948)
                     /locus_tag="PK522_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41125.1"
                     /translation="MKKLMAIALSAMLVAGSTFVFASGNKESKLEPQRAQEILNQMGC
                     KDKKPEETIKDRTDGSIVKCGDVKPLVKR"
     gene            8168..>8448
                     /locus_tag="PK522_05015"
     CDS             8168..>8448
                     /locus_tag="PK522_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41126.1"
                     /translation="MQKYGYGKKLLISFFIGLIGVVLYLLPFGLALDEKFGLNVLFHL
                     RGVTSVPSDVVVIAIDQYSASKLKDNNQIANNTIDPSKWPRALHAELIDR"
     assembly_gap    8449..8548
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8549..10668
                     /locus_tag="PK522_05020"
     CDS             <8549..10668
                     /locus_tag="PK522_05020"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNC41127.1"
                     /translation="TIDPSKWPRALHAELIDRLIEEEVKVIAFDLRFRGCGSSFEDND
                     RLVQAIKNAGNVILVEELVDSRDIADFPYQMDEQPEVCNSESLSTKDLNRDEPHQYYF
                     SESVGLHGAWHRSVLPMISDAAQATAPFVLPRGVVAYHWTFKEDAGGIPLLPAVVLQI
                     FAQDVYDQFAHILQEIDPVAGLNLPSTLDKGIEIEALMYELRGQFIEKPALKDQFINA
                     LNQNDEIDTQTKGKLQALAELYAGHEKHYLNFYGPPRSIRTIPYYQILNQDRLQDQRV
                     DLRGKAVFVGVSAAKFGEQDKLRDDYTTVFKQLDGLQISGVEIAATAFANLLENQTLH
                     PIDEGYAAILVFLFGFMICVFYFALPYRIVFIAVILFLLIYYFSAYQLFETKHLWIPL
                     VIPLIQVGLGFGIALSLKYVESNQQKERLQQAFGKYVPDKVVNDFIHNSGLATARGQQ
                     LYGVCMDTDADRYTELSEKMRPTELMVLMNEYYSRLFESVKACEGLISDVKGDAMLAI
                     WTEPNFSKRQCEQACRAALKIIDAVEVFNRSHTDRALPTRIGLHIGEVVVGNVGTVDH
                     YEYRAVGDLVNSANRIQSANKHFQTRILLSTDVLEGLDLFLTRPLGSVLLHGKSNPIN
                     LAELINFKAKATDDQIWLCEAFAKVIQAYEVQNWIVAKQDLLEILEKFPNDGPSRFFL
                     NRCETYLKVSPVAWDAITYLSGK"
     gene            10865..>14326
                     /locus_tag="PK522_05025"
     CDS             10865..>14326
                     /locus_tag="PK522_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNC41128.1"
                     /translation="MKARGRQTVRVDYRMRWHSVMLALLILLYFAYSRFSVASDIKPN
                     LNYCSEQQAQIVAVEGIAEVLRSGERAWKHIRIGDGLCAGDMLRVRAHSRVALRQKNE
                     SMLRLGEKTTVTFAQSEVHSAQRKATLLELLQGVLHVITRTPEPFEIRTPFMNAGVDG
                     TEFSVAVEPDKTQVVVYEGQVTASNDQGSIVLADQEAGIAYKDQKPQKEVIVRPLDAV
                     QWALYYPAIIDSRVIEQQLPQTLQATVRESLEHYRQGNTFEALAALETLDQNTLSAQL
                     LTYRAGLYLTLGRVAEARHDIAQTLQLQPNDSEAYALQAIITVVQNDKVAALQLAEQA
                     VNADRTSPTARLALSYAYQAHFQIEEALASVEEALRLDGKNALVWARLSELQMSTGYL
                     DRALESAQQAVSLNPNLGKTQTVLGFAHLLQIDTTQAKATFAKAIQLDQADPMPRLGL
                     GLALIREGQLEAGRIELEIAASLDPANSLVRSYLGKAYFEEKRYGLAETQFDLAKARD
                     PNDPTPWFYDAIQKQTQNRPVEALRDIQKSIELNNNRAVYRSKLLLDKDEAARGSSLA
                     RIYDNLGFEKRAIMETAKSLSHDPGNHSAHRFLADAYVNIPRHEIARVSELLQAQLLQ
                     PINVNPVQPRLAVADLNIITGTGPAALGFNEFAPLTERNKPQVVASGLFGSNSTLGNE
                     VVASAVYDKASISVGQFHYDTKGFRQNNDQTHNVYNAFMQYALTPKLNVQAELRTRGT
                     EHGDLIMDFGRQGDPTDPPNFTRNRRALNEDIARVGARYAISPKQDVLFSGQYVNRDE
                     NLKFFSSDDITSKIKDEGYQTEAQHLLRGTFFNLVSGLGAYRTDVNNIRHVDFTRRDG
                     EACKSSRLNNDFCDPDLPGFTRERTNGYVYANVSHLKNMNWTFGLSYDAYKEFRDFDV
                     NSLNPKFGLQANITNFLRLRLAWFETVKSALIANQTLEPTQIAGFNQFFDDVNGTKSQ
                     RKGIGLDANFTKDLYSGVEYSERDLEAPQFNGPGRTTQRIPNSIYTLSSIDKQHEKLF
                     RTYLYWLPISNWVVRSEFQYEHFERDQAKTPPLEVNRINTLSTPINISYFDPSGLFAG
                     MTTTFVLQDIERSAANLPEDQRNSAKLNAGMDSFFLWDTVLGFRMPNRRGVLSLEGRN
                     LLDQDFLY"
BASE COUNT         4106 a         2682 c         3304 g         4134 t
ORIGIN      
        1 cagaagttaa gtcttggatt tgtgtcgtgc caagaccttt gatttgattg gcagtcagtg
       61 cggccacatc tccggtgtcg agcgcggcta tttgatcgct gtcaaggctg ttcaactgag
      121 ctgttgacag cgcagcgacc tgagcgctgg tcaataactg gatattatcg cttgttaaac
      181 ttgatacttg gtcagtagta aggcctttga tctgagtagc agttagtgcc tgaatctgat
      241 cagatgtcat attgttgagc tgatcgcttg taaaggcgac aatttgttta gctgagaatg
      301 cagtaatttg actacttgtc atagcagcaa gatcatccgt ttcaatattg gcgatctgag
      361 tggttgtcaa tgctgcaagt tggttattgc tcaactgagt gatttgactg gtggttaagg
      421 cagcaatttg actgctatcc attcccttga cttgggttgc ggtaaaggca gctacatcgg
      481 caacatcaat ggctgcaatt tggtccgagt caaagctgtt taattgcgtg atggatagtg
      541 aggcgatctg tgccccagtg agtagttgaa cattatcagt cgtcaatgtg gacaattgat
      601 cagtcgagag tcctttgatc tgagtggccg tcagcgactg tatttgatcg gaacttaggt
      661 tgttaaattg ttcagtactg agtgcattaa gttgggctgc gctgagtttg attaagtcac
      721 gagtttctat tgcttggatt tgatcagtcg tcagtaccga caattgatcg gtactcaatg
      781 cggcaatatg agtttggttt aatgcttcga tctggtaagt tagcatgcca tccgcaaatt
      841 cttgagcatc aaaactcttg atctgagtag cgctgagtac gctcagatct tctgtctcaa
      901 tagcgccaat ttgttcactg gtgagtgcat tgatttgtgc ggtagaaagt ttcttgaaat
      961 cgtcggttgt cagactctgg atctgactag ttgtaaaagc ggcaatttgt gccggcgtaa
     1021 atccctgaat tgacataatc aaataccccc tttagatgta tatagtatgt agagaccgta
     1081 aaaaaatgat tgaaatagct aacggtcaat tgaatggctt cttaagaaca ccgatttatt
     1141 aaataccgta cataaatgcc cgcattttgc aagctaatca atcagctgat cccaaattag
     1201 ttattacatc aggattaccc tagccgcagg cggtaaattg tccttggttt ttgatattaa
     1261 tctccctgga tcgcattgga cttacaggaa cctttatctc gggagggcag aacgatgaaa
     1321 gaatgaggca atccagagtg atttattttg tttttgtttt acgaatatca aaaataatgg
     1381 gataaacctt tgtttgttta ttcaactgag attggaattc gtcactgata atagcttcca
     1441 atgacgggag cttattatgg cggatgaaag tgatttagaa aaaacggaag aggcaacgcc
     1501 caggcgactt gaaaaggcca gagaagaagg tcaggttgcg cgctcccaag aattgactac
     1561 gtttgcttta ttgatggttg ctggtggggc cttgtggcta ctcggatcaa gttttattac
     1621 gaatttaatg gaaattttga aacgaggttt gcaaatccaa cctgaagcgc tattagatag
     1681 cacgtcgcta cctgttcggt tatttcaaca tgcgattgat agcttaattg catcagcgcc
     1741 tttgtttgca gtttttgtga tcgttgctgc gattgcaccc actttgctga gtggactgct
     1801 atttactacg aaagcattaa tgcctgattt taagcgtctc agcccaattc aagggtttaa
     1861 aagaattttc tccatgcatg gttttgttga gttagtgaag gccattgtaa aaacaatcgt
     1921 ggttggcgga gttactgcat tgttgatttg gtacaacaag gaagcggtaa ttggtttgat
     1981 tttgttgcca attgatctag gtgcggaaag tatgggtagc ttgttgttac tgatttttat
     2041 gctaattgct gctgccatgg tgttgatcgt tggtatcgat gtacctttcc aaatctggca
     2101 acataagcac cgcctaaaaa tgaccaaaga ggagattcgt aaagaatcaa aagaagacga
     2161 aggagatcct tttgttaaag cccgtatcag gagcctacaa cgagaagcag ctcgtcaacg
     2221 aatgatggct gaagtaccta aggctgatgt aattgttact aacccgaccc attatgcagt
     2281 tgcgttgaaa tatcatggag aatcaatgcg tgctccaaga gtggtggcca aaggggtaca
     2341 tttacttgcg caacgcattc gtgagattgc cgaagaaaat cgtgtgccgg tactcgaggt
     2401 gccacccctt gcgcgtgcgt tgtatcacca tactgaattg aatgatgaaa ttcctgaaac
     2461 actttatacc gctgttgcgc aaatattagc ttatgttttt caattgaagc gctaccagac
     2521 ttatggtggt gtggtaccgc agctaccgag tgatttaaca gtgccgcagg agcttgatca
     2581 tgcttaatga agttgtgaac gttgttcaat aagttaaccg gttctaaatc atcagggaaa
     2641 tccatattgt tatgataaac cactctttat cgctgacatc gttgatcgat aaaaacaact
     2701 tgcaaagcat tgcaggtcca ttattgatca ttatgattct ggcaatgatg gtattaccgt
     2761 taccgccctt tttccttgat gtattgttta cattcaatat tgcactttca attatcgtac
     2821 tgcttgtaag cctttacacc aaggatgctt tagattttgt agcatttcca acggtactgc
     2881 tgattaccac gttattgcga ttatcgctaa atgttgcttc gactcgggtt gtattaatgg
     2941 aagggcatag cggtccagat gccgcaggta aagttattga agcatttggg cattttctgg
     3001 ttggtggtaa tttcgcagtc ggaattgtcg tgtttatcat tttggtggtg attaactttg
     3061 ttgttataac caaaggtgcc ggtcgtattg ctgaagtgag cgcacggttt acgttggatg
     3121 caatgccggg caagcagatg gcaatcgatg ctgatttgaa cgcaggtctg atcggagaag
     3181 ctgaagccaa aaaacggcgc gcaacgattg cgcaggaagc tgatttttat ggatcgatgg
     3241 acggtgcaag taagttcgtt cgcggggatg caatcgccgg tatcctgatt atggttatta
     3301 ccattattgg gggattggtt gtcggggtat ttcagcatga ccttgatgtg agtgcagccg
     3361 caaagaatta cacgatatta acgattggtg atggattggt tgcgcaaata ccttcattga
     3421 ttatttcaac agcagccggt atggtggtca gccgcgtgag ttctgatcag gatttaggtc
     3481 agcaagttgt cagtcagtta tttaataagc cacaagtatt atttctaact gcaggtatct
     3541 tgggtgtgat gggtttgatt ccgggtatgc ctaattttgt ctttttattg ctagcatcca
     3601 tcatagccgg tatcgcttat tttaaagtta actatctacc ggaaccagaa gcagttactg
     3661 aagaggctac ctcatcccca gttgccgaaa ctgaggggcg agaagctagt tggaatgatg
     3721 tgatgcctgt cgatatgctg ggtttggaag ttggttatcg gttgattcca ttggtcgata
     3781 aaacacaaca aggtgatcta ttaaagcgaa ttaatggtat tcgcaaaaaa tttgcgcaag
     3841 aagtggggtt cttgccacct gttgttcata ttcgagataa cctagagcta agacctaatg
     3901 cttaccgaat tacgctgaaa ggttctgtca ttgggcaagg agaatcttat aacggaatgt
     3961 atctggcgat taatcccggt caagcgacga agacattatc tggtgtgaat accagtgatc
     4021 cggcttttgg attgcctgct gtatggattg aacctgcctt aaaagatgat gctcaggcac
     4081 atggttatac cgtcgttgat ccgagtaccg tggttaccac ccatgtaaac catttaatgc
     4141 gctcctatgc agcagagcta ctgggtagag aagaactaca gaaactcttt gatcacttgg
     4201 gtcgcagtat acctaaacta accgaagact taataccgaa gctattacct ttagcaacgg
     4261 ttcagaagat tttgcaaaat ttattagaag aaggcgttca tatacgggat gttcgtacca
     4321 ttgttgatgt actcacagaa catgctaccc gtactcaaga taccaatgag ttaacagcgc
     4381 ttgtgcgagt tgcactaggg cgttccattc tggagcagtt tttcccttca ggtgaagaat
     4441 tacaagtgat gagtcttcat cacaatttag aaagcatttt agtacaagtt gcacaatcag
     4501 ggcttgttgc gggtgctgga atagagccag gcttggctga tacgttattg aaggaagtca
     4561 gtaaagccgt cgatcaacat gaacagcttg gaattcccgc agttttgcta gtgcctgatt
     4621 caatccgatt attattggcg cgtttcttgc gccgcccatt accacaactc aaagtgattt
     4681 cacatgctga ggtagtggat tctagaaaaa ttaaatttgt atcgatcgtt ggagggaata
     4741 aatgaaaatg agaagattta ttgcgaaaac ttcacacgag gcgttacgca atgttaagga
     4801 agaactgggt gctgatgcag ttatcttgtc gaataaacaa gtcgatgaag gcattgaaat
     4861 tgttgcattg gcaagtaatg aaatgtcaag cttggtcaat agtcccgaat ctaagtttgt
     4921 tccaacaccg ccccccgtcg aaaagaaaga ggaagtatta tcggttatta acccaaatat
     4981 tgaagtgcta gaagcgattc aacaagttaa gaaagaatca aatatttctg aagcgcttgc
     5041 actgaatatc attaatgaaa ttcaggcaat gcgccataca ttggaagatc aactgaattc
     5101 agttgcttgg ggaagtttgt cacaaagtaa accggaaacg atgaaactga tgcgaacact
     5161 gcttgatgca gggtttagtc cattattatc gcgccattta cttgagaagc taaccaaagt
     5221 tagtaattac gaagagagtt taaagcagac ggttgcgcta ctaacgctca atttacgcac
     5281 tgcaacgagt gatcaactga tagagaatgg cggtgtttat gcactgattg gtcctactgg
     5341 ggttggaaaa acaacgacga ttgccaaact tgctgccaga gctgtcatta ggcatggcgc
     5401 agataaagtt gcattactga ctacggatag ctaccggatt ggtggacacg agcagttgag
     5461 aatttatggc aagttattgg gaattccagt tcgtagtata aaagacattg atgatttgca
     5521 attaacccta cttgagctgc ggagtaaaca tattgtactc atcgatacgg ttggtatgag
     5581 tcagcgtgat cagatggtta gcgaacagat ggccttattg cgggactgtg gaactgaaat
     5641 taagcacttg ctcttgttaa atgctacgag taatggagga acattagacg aggtgatctc
     5701 tgcctaccaa tataatggga tcgaaggttg tattattacc aaggttgatg aggcggtggg
     5761 tttaggtacg gcccttgatg ttgttattcg acgtaagctc gtgttgcatt acgtaaccaa
     5821 tggacagaaa gtaccagagg atctgcatgc cgcaaattca agatatttac ttcataaaat
     5881 tttcaaaccc atcgcagaaa attccccttt ttctttgcag gatccagaat ttgcattagt
     5941 aatggcaggc tatgaccgga atagtgcagc aaaaccaaag ggatacgctg aggctagcta
     6001 tgagtagaaa aatgattgac caagcatcgg gtttgcgaga gctcgctgca atgcaggcac
     6061 gtgactcagt aaaagtgttt acggtcacag gtggcaaaat gggtgttggc aaaacgagta
     6121 tcgtggtcaa tcttgctgct gcgctggcca aaattggaaa tcgcattctc atcctggatg
     6181 agaatcaaca tcacaataat gtcagtgcta atttaggtgt caaaagtcat tatgacttga
     6241 cccatctaat caagcgtgaa cagtcactgg atcaaattct ggcaaaagcg ccagatgata
     6301 ttttagtact tactgctgca cgaggcattc attcattgac tcaattgtca gctgaagagc
     6361 aagagtggat tattaaatgc ctgtgcgaat tacccatcga tgtagtgctc gtcgatacca
     6421 tgattggaga gatgggtcat gtgttgccac ttagcctagc ctcgcagcaa gtgctgataa
     6481 cggtatctgg atcatcaaaa tcgattacag atgcctatac actgattaaa atcatgagcc
     6541 aagaatatgc gcggcaggat ttcttagtaa tcgtaaataa ggttaagtct gagcttgatg
     6601 cgcagagcat ctatgaaaat atcgcaaaag ttgcaaaaca gcatttgtct gtaagacttg
     6661 agtatctggg ttgtatttta tttgatgaga aattacatcg tgcaagccaa ttatgtcgat
     6721 cggtagtcga tgcatttcct gcttcgactg ctgcaattga atttaagcgg ctggctgaga
     6781 aattattacg atgttcctat tccaatcaat atagcggtgg cgttgataat tttatgtatc
     6841 gattgattcg aacaagccat ttaagcaaag taaaactttc agtctaatta gagcctatgt
     6901 atacaacttc tggaattatt gataaagagc aatttgttgt cgaatttgct ccactcgtga
     6961 aacgaattgc ccatcatatg atgatgaagc taccagccag tgttgaagtc gatgacctga
     7021 ttcaagctgg aatgatcggc ttactcgatg cgattaatcg ttacgaaggt gcttatgggc
     7081 ggcagtttga aagctatgcc gcacagcgga tccggggatc aatactggat gaattacgtg
     7141 aatcagattg gttaccgcgt agtctgcgaa aaaaaatgcg acaaatcgaa gtagcaatgc
     7201 gttcactgga gcaaaagcta ggctgtcctc catcggagca agagatagct aaagagttaa
     7261 atatatccct ggaagaatac catgaagcac ttcaagatgc gcgtggcggg cagctaatct
     7321 attatgagga ttttcaggat gatgatgaag atcactttct tgatcgtcta tgctcagatt
     7381 ctgggaatga gccgcttgaa atattggtag ataagaatct acgtgaactt ttagtagcgg
     7441 caatcgataa gttaccgatt agagaaaaag aggtcatggg gatgcactac gagcaagaaa
     7501 tgaacttacg tgaaataggg gaggtactcg gtgtgagtga gtcacgagta tgccagattc
     7561 actcccaagc aattgctcga ctacgtgcta agctaagaaa tcaatagaga atgattaaga
     7621 aagttttatt gaagatgacg aattaaaagt ctaattgaac cggctttcta ggctatcaca
     7681 agcgaaacga tcattacctc gcaatagcct aaaagaagaa tttattttat cgttttacta
     7741 gcggtttaac atcaccacat tttacaattg atccatccgt acgatctttg atcgtttctt
     7801 caggtttttt gtctttacag cccatttgat tcaatatttc ttgcgcgcgt tgtggttcaa
     7861 gtttagattc tttatttcca cttgcaaata caaaggtgga tccagcgacc aacatagctg
     7921 aaagtgcaat tgccatcaat tttttcattt tttgctcctt ttaatttatt gaagttaata
     7981 aatacccacc agatacttcc aatacaataa cctagtttgg ctataaattt agttaaatct
     8041 actctctaga tccaatatct acttatactt tttaatactt gtgtagctat gatataacag
     8101 tgtgggttgt ttgtcaggtg taaattttag tgttttacca tctaattcta tgtgatttgc
     8161 tctcattgtg cagaagtacg gatacggtaa aaagctgctg atctcatttt ttattgggct
     8221 aattggagtt gttttatatc tattaccctt cgggttagca cttgatgaaa aatttgggtt
     8281 gaatgttctt ttccatctgc gcggcgttac ctcagttcct tctgatgtgg ttgtgatcgc
     8341 gatcgatcaa tactcagctt cgaagctaaa agataataat caaattgcga ataatacaat
     8401 cgatccaagt aaatggcctc gtgcgctcca tgctgaattg attgatcgnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnat acaatcgatc caagtaaatg gcctcgtgcg
     8581 ctccatgctg aattgattga tcgtctcatt gaagaagaag ttaaagtgat cgcatttgat
     8641 ttaaggttta gagggtgcgg aagtagtttt gaagataatg ataggttagt tcaagcgatc
     8701 aagaatgcag gtaacgttat tcttgttgaa gaattagttg attccaggga cattgctgat
     8761 tttccctatc aaatggatga acaacctgaa gtttgtaatt ctgaatcgct atcaacgaaa
     8821 gacttgaatc gagatgagcc tcatcaatat tattttagtg aatcggtagg gttacatgga
     8881 gcatggcatc gatccgtact accaatgatt tccgatgcgg cacaagctac ggctcctttt
     8941 gttttgcctc gaggagtagt ggcttatcac tggactttta aagaggatgc gggaggaatt
     9001 cctttattac ctgcagtggt gttacagatt tttgcacagg atgtctatga tcagtttgct
     9061 cacatactac aagaaatcga tcctgtcgct ggattgaatc tgccttccac cctggacaag
     9121 ggtatcgaaa ttgaagcact aatgtacgag cttcgtgggc agtttatcga aaaaccagca
     9181 ttaaaagatc agttcattaa tgcgctaaac caaaatgatg aaatcgatac tcagacaaag
     9241 ggtaaattac aggcgctagc tgaactctat gccggccatg agaaacacta tttaaatttt
     9301 tatggccctc ctaggtctat tcgaaccata ccctactatc aaatactaaa tcaagatcgt
     9361 ttgcaggatc agcgagttga cttgaggggg aaagctgttt ttgtgggtgt atcagcagct
     9421 aagtttggtg agcaagataa actgagagac gactatacaa ctgtttttaa acaattggat
     9481 gggttgcaga tcagtggggt agaaattgca gcaactgctt ttgcaaactt gttagaaaac
     9541 caaacattac atcccattga tgaagggtat gcagcgatac tcgtgttttt gtttggtttt
     9601 atgatttgcg ttttttactt cgccttacca tacagaatcg ttttcattgc agttatcctg
     9661 ttcctcttaa tttattattt ttcagcgtat cagttgtttg aaactaagca tttgtggata
     9721 ccattagtga ttcctttgat acaagtaggc ttagggtttg gtattgcttt atcactaaaa
     9781 tacgttgaat cgaatcagca aaaagaacgg cttcaacaag ccttcgggaa atatgtacct
     9841 gataaagtgg tgaatgattt tattcataat tcgggattgg caacggctag agggcagcag
     9901 ttatacggtg tctgtatgga taccgatgct gatcggtata cggaattaag tgagaagatg
     9961 cgcccgactg aactcatggt cttgatgaac gagtattact caaggttatt tgagtctgtt
    10021 aaagcatgtg aggggctcat ttcagatgta aaaggtgatg ccatgcttgc gatttggaca
    10081 gagcctaatt tttcgaagcg acagtgcgag caagcctgca gagcggccct taaaattatc
    10141 gatgcagtcg aagtattcaa tcgaagccat actgataggg cgctacctac ccggattgga
    10201 cttcatattg gggaggtagt ggtcggaaat gttggaacgg tcgatcatta tgaatatcgc
    10261 gcagtgggtg atttagtgaa ttcagccaat cgaatccaaa gcgcaaataa acatttccaa
    10321 actcggatac tgctttctac tgatgtactc gaaggattag atctttttct gactcgccct
    10381 ttgggaagcg tgttgctgca tggaaaatcg aacccaatca atcttgctga attgattaac
    10441 tttaaagcga aggcgactga cgaccaaatt tggctttgtg aggcctttgc taaggttatt
    10501 caggcctatg aagttcagaa ttggattgtt gcaaaacaag acttactcga gattctagaa
    10561 aaattcccta atgatggtcc ttctcggttt tttttgaatc gttgtgaaac atatttgaaa
    10621 gtttcacccg tcgcttggga tgcaatcacc tatctttcag gaaaatagat gatcgattta
    10681 aaagctatgt cttaaaacta tcatcatact tagttataaa aagaatcagt gagaaaaatc
    10741 acacgctgaa tgaaaaggat cggatatgct attatcaata atgcagatat ggtttctctc
    10801 ggagaagcaa aataagaagg caaacgatct tcaccaaatg aataatattg attaggttgc
    10861 aattatgaaa gcgagaggga ggcaaacagt tagagtagat tatcgaatgc gatggcattc
    10921 tgtgatgctc gcgttactta tactccttta ttttgcttat tcacggtttt cagttgcaag
    10981 tgacatcaag cctaatttga actactgttc agagcaacaa gctcagattg ttgccgtaga
    11041 aggcattgct gaagtcctta ggtcagggga acgagcctgg aagcatatca ggatcggtga
    11101 tggattgtgt gcaggtgata tgttgagggt gcgcgctcat agccgagtgg cgttaaggca
    11161 aaaaaatgaa agtatgttgc gcttaggtga aaagacgacc gtgaccttcg cacaatctga
    11221 ggtgcattct gcacaaagaa aggcaacact gctggaatta ctgcagggtg tattgcacgt
    11281 tataacccgc acacctgaac cgtttgagat tagaacacct ttcatgaatg cgggtgtgga
    11341 tggaactgaa tttagtgtag cagttgagcc cgataaaacc caggttgtgg tttatgaagg
    11401 tcaagttact gcaagcaatg atcaaggtag cattgtatta gccgaccaag aagcaggaat
    11461 tgcctacaaa gatcagaaac cccaaaaaga agtcatcgtt cgacccctcg atgcggtgca
    11521 atgggcgtta tattacccag cgatcatcga ttctcgcgtc atcgagcagc aactccctca
    11581 aactttgcaa gcaaccgttc gtgaatcatt ggaacactac cgccagggca atacatttga
    11641 agcgctcgct gcactcgaaa cattagatca aaataccctt agcgcgcagc tcctcactta
    11701 ccgtgccgga ctctatttaa cactgggacg cgtggcagaa gcgcgacacg atatcgccca
    11761 aacgctacaa ctgcaaccca atgatagcga agcttatgcc ttacaagcca tcatcaccgt
    11821 ggtgcaaaac gataaagtag ccgcattgca actcgctgaa caagcggtta atgctgatcg
    11881 tacttcaccg accgcacggt tagcactttc ttatgcctat caagcccatt ttcaaattga
    11941 agaagcactc gccagtgtcg aagaagcgct gcgcctcgat ggcaagaatg cattggtttg
    12001 ggcacggcta tcagagttac aaatgtccac cggttatctc gaccgcgcat tagaatccgc
    12061 acaacaagca gtaagtctta atcctaattt aggtaaaacc caaaccgtat taggatttgc
    12121 tcatctatta caaatcgata ccacccaagc gaaagcaaca ttcgctaaag ccattcagct
    12181 tgatcaagcc gatcccatgc caaggcttgg gttagggctt gcacttattc gtgaaggcca
    12241 acttgaagca gggcgcatcg agcttgaaat tgctgcaagt ttagaccctg ccaattccct
    12301 agtacgcagc tacttaggta aagcctactt tgaagaaaag cgctatggac ttgcagagac
    12361 ccaattcgat ctggccaaag cacgcgatcc taatgaccca accccgtggt tttatgatgc
    12421 aattcagaag caaacgcaga accgaccggt agaagcatta cgcgacattc aaaaatccat
    12481 cgagctcaat aataatcgtg ctgtttatcg ctccaaatta ctcctggaca aagacgaagc
    12541 cgccagaggc tctagccttg ccagaatcta cgacaattta gggtttgaga aacgtgccat
    12601 catggaaaca gcgaaatcat taagccatga tccaggaaat cattccgcgc atcgattctt
    12661 ggccgatgct tatgtaaata taccgcgcca tgagattgca cgcgtcagtg agttactgca
    12721 agcacaattg ctacaaccga ttaatgttaa cccagtgcaa ccaagattag cggtcgcaga
    12781 tttaaacatt attaccggaa caggccctgc agcgttaggg tttaatgaat ttgcgccatt
    12841 gacggaacgt aataagcccc aagtggttgc atcagggttg tttgggtcaa acagtacgct
    12901 aggcaacgaa gtcgtagctt ctgcagttta tgacaaagca tcgattagcg tggggcagtt
    12961 ccactatgat accaaggggt tccggcaaaa taatgatcaa acccataatg tttacaatgc
    13021 attcatgcag tatgctttga cccctaaatt gaatgtgcaa gctgaactca gaacacgcgg
    13081 aactgaacat ggggatttaa tcatggattt tggaagacaa ggtgatccta cagatccacc
    13141 taattttact cgcaacagaa gagcattgaa tgaagatatt gctcgggttg gtgctaggta
    13201 tgcgatatct cctaagcaag atgtactatt ttctgggcaa tacgttaacc gtgatgaaaa
    13261 tttaaagttt tttagtagtg atgatataac cagcaagatt aaagatgagg gttatcaaac
    13321 tgaagcacaa catttattgc ggggtacctt ttttaatctt gtaagtggcc ttggcgctta
    13381 tcgtaccgat gttaacaata ttcggcatgt tgattttacc aggagagatg gtgaagcgtg
    13441 taagtcaagt aggctaaaca atgatttctg tgatcctgat cttccaggat ttactcgaga
    13501 aagaacaaac ggttatgtct atgcaaatgt tagtcatctc aaaaatatga actggacttt
    13561 cgggcttagt tatgatgcgt acaaagaatt tagagatttt gatgttaata gtttaaaccc
    13621 taaattcggg ttacaagcta acataaccaa ttttctacga ttgaggcttg cttggtttga
    13681 aacggttaaa tcagcattga ttgctaatca aacgttagaa ccaactcaaa tcgccggttt
    13741 taatcaattt tttgacgatg tgaatggcac gaaatcacaa cgaaaaggaa ttgggctgga
    13801 tgcgaatttt acgaaagatt tatatagcgg tgtggaatat tctgagcgtg atcttgaggc
    13861 gccgcaattt aatgggccag ggcgtactac tcagagaata ccaaattcta tctatacatt
    13921 aagtagtatt gataaacagc atgagaagct gttccgtact tatttgtatt ggcttcccat
    13981 ttcgaattgg gtagttagaa gtgagtttca atatgagcat tttgagcgtg atcaagcaaa
    14041 aacaccacct ttagaggtaa accggataaa tacattgagc actccaatca acataagtta
    14101 cttcgatcct tctggcttgt ttgcgggaat gacaacaacg tttgtactac aagacattga
    14161 acgatcagct gcaaatttgc ctgaagatca gcggaattca gctaagttga atgctggtat
    14221 ggatagcttc tttttatggg atacagtatt gggttttaga atgcctaatc ggagaggtgt
    14281 tctgagttta gaaggaagaa atttattaga tcaagatttt ttatat
//
LOCUS       DAOQLJ010000048        14296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1555_length_14296_cov_6.168176, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000048 DAOQLJ010000000
VERSION     DAOQLJ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 14296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14296
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1555_length_14296_cov_6.168176"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PK522_05030"
     CDS             complement(<1..565)
                     /locus_tag="PK522_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41129.1"
                     /translation="MNIKYQIKNLLLATSITIGLSATSAMANVVGFMGLELDGCFSSN
                     ANTAVCGPHENNAAVFATGTAVFNESLLSRFGGTAGLATQPFSYAGFGELLVGTSPGA
                     PLAPFADITRTWADYTALSSDPNLMLGFAILNQIAAHPNDASSSFTFDLTPNPGDEFS
                     IFWSITNIVTNGSVTTGDWKAWSDDVLP"
     gene            963..2066
                     /locus_tag="PK522_05035"
     CDS             963..2066
                     /locus_tag="PK522_05035"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNC41130.1"
                     /translation="MKTKPKSNHSLASVLMKMILIMMLGLGVWLAYEVFYLQPADNWF
                     EVKAKTSSSVNLPKDDAPHLAKMEWWYYNGHLWTESGKQFSFHYTTFMVTELVSHLVK
                     QVSLSDHQTGKHYTDQKRTTGGGFSSNIENGFEFLLDNWKMVGDDGNDQLIVSTPQFS
                     FNLKLTNTIPPVFHGTDGIIFLEEAGNSYYYSRTRMDITGTIKIGNTTETVRGLSWFD
                     HQWGDFSTNLLSWDWFSLQLDNGIDLMIYQLRDKYDKPLLYTGSITQNNVTEALLSSD
                     FTLSQLGDQWTSKKTGYTYPIAWEIKIPKKNIHIKTRSKVENSEFDAQLTTYNHYWEG
                     AVKIEGTHTGKGFMELYGYTNKKEPNASRSKKR"
     gene            2070..2510
                     /locus_tag="PK522_05040"
     CDS             2070..2510
                     /locus_tag="PK522_05040"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNC41131.1"
                     /translation="MFTNILLVCVGNICRSPMAEGLLKHHLAQSAQTNHFVTSAGLNA
                     LVGHKADEKACHVLIQKGIDISSHRACQLNSVMVRKADLILVMETSHKKAIEDNEPGA
                     RGKVFRLGEWGKFEIADPYQKDLSIFEDSALLIEQGINQWLTKL"
     gene            2625..3857
                     /locus_tag="PK522_05045"
     CDS             2625..3857
                     /locus_tag="PK522_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HNC41132.1"
                     /translation="MHLKIFLVLAACALPACSIVPGQHMSPFSRQSSVKMPVTENNET
                     ILKTLDIQTINAELVLALERDFNTRSLGPDNVANHYFDYRIGSRTIKGVPMETEPYTQ
                     YRVGPRDILNITVWDHPELTIPAGQFRAAEKAGNVVGEDGTFFYPYVGIVHAAGRTVE
                     EIRDELTERLSRYIENVQLDVRVADYRSQRVYVVGEVAQPGIQLVKDIPLTVLEAINN
                     AGGVKSEADQRNITLTRDDKTYSINLLSLYEGGDITQNVLLKHGDILNVPDSSLNKVF
                     ILGETNFRVLGGAGIGRSRSLVMNKARMTLTEALSDAGGFDQETSDPARIFVFRGGVG
                     KSEIFHLDAKSPDALLLADRFPLQPRDVIYVDRAEGIRWNQIIAQIQPTVNLLNSFDG
                     VLRIQPFRAFPSAPSTVR"
     gene            3985..6249
                     /locus_tag="PK522_05050"
     CDS             3985..6249
                     /locus_tag="PK522_05050"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNC41133.1"
                     /translation="MSNLPTDRSPTAFDYSLNQDEDVINLGELFGTLYESKWLILLIM
                     TVTVLIGFAKTQLDRPIYKADGLLQVKENSTSMAGLEPLAGLIEGKMAVMAEIELIKS
                     RMILGKTIKSLHLDVIAKPRYFPIVGETIARQFQNRNQDGSVSNPLFGLTQYAWGGET
                     IQVETFIVPSILEDEEFILKTDQEGFFKLFYKDEIILEGEVGKLANKQIEGYSQPIAI
                     FVSLLKSRPETHFTIVRQSENQAIRQLKERISIAEKGKNTGMLELTVESSLPDAAVSV
                     LNEIANIYVQQNVDQKSAESQKTLEFLEKQLPILKDQLDAATTSLNEYRTRKGSVDLN
                     LEMQNILKNSVELKTQTTILQQKRDELRQRFTESHPHIIAIDKQIARLQGQISTQDKK
                     IETLPETQQAILKLSRDVKVSTELYTTLLNNAQTLRIAKAGTIGNVRIIDYAILPDEP
                     IRPRKVLIIGIALVLGIIFGVVTALIRKSLNRGIEDPDLIEKLLNTPVYATIPHSKFQ
                     KALSNKLKHNQANNVASPVILALENKEDVAIESLRSLRTTLHFAFLEAQNNIIMITGP
                     SPGIGKTFVSVNLAVVMADAGKKLLLIDGDMRRGYINKVLGVNRENGLSELIFESISL
                     DKAIHPIESAHFDFIATGAIPPNPSELLLHERFANLLDNLSKQYDLVIIDSPPILAVT
                     DAAVIGRLAGATLMVIKAGQHPARELEQSTKRLIQAGVNLKGIVFNDLPELASRPGYG
                     YGKYVYQYSYKSGK"
     gene            complement(6267..7919)
                     /locus_tag="PK522_05055"
     CDS             complement(6267..7919)
                     /locus_tag="PK522_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNC41134.1"
                     /translation="MTEVTDSTPTAIFRTPYILSNRKQPAEIKGTRPTETEYGVLDPK
                     TFDAVEVDALFEGMNHAETQIGQAVLYRSLAQPTHHVEQLHKKQEALRELAMNPPLLD
                     ACEQFIKKMADGEETLYHLLYGEFSGGFTTDHPKNRVGKLEFGGYGYRQFIDGTEFAV
                     DLVEEAGELPPVQSAYLQELLQAIRDFGGSKTYALMHGPFYVIDGKFKTKDEKPPYLP
                     IPRFRPSMFKWLPSLIFVLIVSGLLYFFENMLVELGANYIGYGILILTVPIFPIIVQA
                     VSSSDRDSVIYPLQKLFRQSSELAKAMEALGMLDELLSLHRYAKASHHEMVLPEIMTA
                     GQHTLTAIQAKNPLLLNTNTHYVANDVKLDAANRLLIITGPNSGGKTAYCKMIVQLQI
                     LGQAGSYLPASMARIVPAEHIFYQIPDAGKLEEGMGRFAHELKQTREIFFNSTSLSLV
                     ILDELAEGTTFEEKMTLSEYVLKGFHQLGASTLLVTHNHELCERLLSEGIGSYLQVEF
                     LQGEPTHRLIPGISRVSHADRVASAIGFSKADVEKHLESLKS"
     gene            8075..8629
                     /locus_tag="PK522_05060"
     CDS             8075..8629
                     /locus_tag="PK522_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNC41135.1"
                     /translation="MPRKFFKKYLPSHESIQQNRFISFFGELLHHHNLWHLHRRSVAG
                     GFAAGLFAGLIPGSNPVQFFFAALFSITFKVNLPVAALTTLYSNPFTILPLYWTAYLL
                     GGFFIDNGMNNLPPLELNLADKSFSEWMPAVVTWLTSFGKPLIIGLVLLASLLAIIGY
                     FLVRLIWRFYVIHAWHKRHQRPQP"
     gene            8698..9852
                     /locus_tag="PK522_05065"
     CDS             8698..9852
                     /locus_tag="PK522_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC41136.1"
                     /translation="MQKNKIISRLLIILVTIMVAGYIGWHLSRKPPVEVELATASHGT
                     VEAIVVNTRAGTIKACRRAKLAPAAGGQIVKLLVKEGERVVQGQRLLELWNADLAAQH
                     ELAAQQLATTKSRQRENCIIANNAQRESIRTQQLVAKGFVSSQRAEDAEANAQAQQAS
                     CAAAGADVKRAQAQLEVTQANLNRTLLNAPFSGVIAQVTGELGEYTTPSPPGIPTPPA
                     IDLIDDSCLYVSAPMDEIDAPKLAIGQKARITLDALTNQTFAGVVRRIAPYVTEIEKQ
                     ARTVDVEVNFVENSNQVLLVGYSADVEVITDQREQVLRIPTQAIRQRNKVWALGPQDR
                     LEERTLEIGLSNWAYTEIISGLQAGDQVLIATDIETIVPGIQVKPKGPPS"
     gene            9849..10520
                     /locus_tag="PK522_05070"
     CDS             9849..10520
                     /locus_tag="PK522_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC41137.1"
                     /translation="MIELLGICRTFQMGDQAIHALNQINLKITAGEYVSIMGPSGSGK
                     STLLNVIGLLDQPNSGRYLLDGSDVTHLSESEQARVRRDKIGFVFQSFHLIPRLTAAE
                     NIELPLILSGITPSERKQRVEAALLAFNLVERAQHRPAELSGGQRQRVAIARATVLRP
                     TALLADEPTGNLDHRIGSEVIALLENLHHSGTTLIIVTHDRELGLRAHRRIAMRDGSI
                     IADEQ"
     gene            10517..11719
                     /locus_tag="PK522_05075"
     CDS             10517..11719
                     /locus_tag="PK522_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC41138.1"
                     /translation="MKLSDILQFSLNTLRSYPIRSFLIVLAMALGVAAVVVLTALGDG
                     ARRYVIQQFSSIGTNLIVVLPGRAETAGSFPGAAMGQTPRELTLEDARWLGYIPQIRR
                     YAPLNVGAAELAGAGRLREVTVLGSTVDLLPIRHMELAQGGFLNQGSENNAQIVLGAK
                     LAKEFFPDGNAVGQRVRLADRRFIISGVLASQGETMGFNSDEIVIIPIYHAQALFNTT
                     SLFRILIEVRNRSDIPTTKQAIQEMLIQRHDGEDDVTVITQDAVLSTFDRILNALTLG
                     VAGIAAISLAVAGILVMNVMLVSVSQRTAEIGLFKAIGAQSHDIRYLFLAEATWLSLV
                     GAIIGFILGQFGSLLIRIAYPLLPAWPPIWACIAGIVVAISTGIIAGILPAVRASRLD
                     PVQALSSK"
     gene            11716..12915
                     /locus_tag="PK522_05080"
     CDS             11716..12915
                     /locus_tag="PK522_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC41139.1"
                     /translation="MNFIESLQFALHALSAHRGRTILSASGIAIGIAAVLLLTSIGEG
                     IQRFVISEFTQFGTNILNITPGKIRAHGGSVGSIGSARILTIEDALALKTSRYVQYIN
                     ATVFGNAEIRAEGLSRRVTVYGQGPDFDKAFNMNVAIGQYLPTDDPRNPRAFAVLGAK
                     VHQELFGTMSPLGAILQVGSSRFRVIGVMAAKGQVLGFDLDDTVFIPTARALEVFNRE
                     GVMEIQAVYPPEFPVNLVTEDLQRILIERHGREDFTITPQKQMLSTLSTVLKVLTFAV
                     AALGGISLLVGAVGMVTLMHITVTERVAEIGLLNALGATRARIRTLFLLESTILSTIG
                     GLAGLIIGASIAFLLKIFVSALPVNIPWQFVSIALGISTLIGLIAGVFPAIQAARLNP
                     IDALRSE"
     gene            complement(12973..13896)
                     /locus_tag="PK522_05085"
     CDS             complement(12973..13896)
                     /locus_tag="PK522_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNC41140.1"
                     /translation="MLKLPERNRIYQNHHFDSTRWDYFEPRSTDIIVATSYKAGTTWT
                     QAIVAHLLFQDGNFPAPPAEMSPWLDMRIMPLEVILNRLKAQNHRRFIKTHLPLDGMI
                     YHPHFKYLYIARDTRDVFMSLWNHYTGMKEEIFALMNGLPGRVGEELPHPPENIHTFW
                     QNWITRGGFAWENDGWPYWSHLSNVQSWWNFRHLPNIQLFHYSDMLENTEREMRRMAE
                     FLEIEVPEDTWPRIVKAVSFNEMKREGEKYAPGGGHFWKGGAQTFLHKGSNGRWRDVL
                     SEPELLLYDQACQRALTPDCREWLENGGRIM"
     gene            complement(13952..>14296)
                     /locus_tag="PK522_05090"
     CDS             complement(13952..>14296)
                     /locus_tag="PK522_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HNC41141.1"
                     /translation="QLVPFLDARWLKPGAFATVTDLAAPWIKQSFSELDYIVIDDREQ
                     EAVMETPLINPHWITGDLTELVCDVIKGRSTDEQKTAFIFRGLALGDLALSALAYQTA
                     QLQQIGFPIKRE"
BASE COUNT         4119 a         3228 c         3007 g         3942 t
ORIGIN      
        1 atggtaagac atcatcagac caagctttcc aatctcccgt tgtgacagaa ccattggtaa
       61 caatgttcgt aatactccaa aaaatagaga actcatcccc tggattcggc gttaagtcga
      121 aagtaaaaga actgctcgca tcattcggat gtgccgcaat ctgattcaga atcgcaaatc
      181 caagcatgag attaggatca cttgacaatg ccgtataatc agcccaggtt cttgttatat
      241 ctgcaaacgg tgcaagtggt gcgcctgggc tagtgcctac taataattca ccaaatcctg
      301 cataactaaa aggttgagtc gcgagtcctg cagtaccgcc aaatctactt aatagtgact
      361 cattaaatac agcagtaccc gttgcaaaaa ctgcagcatt attctcatga ggaccacata
      421 ctgccgtgtt tgcattcgat gaaaaacatc catccaattc caagcccata aatccaacaa
      481 cgttagccat agctgaagtt gctgatagcc ctatcgtgat agaagtcgct agtagtaagt
      541 ttttaatctg atactttata ttcattcgag catcctccaa cagttattag aattagatat
      601 ctttatcaat aacgcagcca ataaattcag gattcaatta acccattttt tatttatttt
      661 tcagctagcc agcctaattt acacaacaaa tgttacaaga atttacatct aatatagtct
      721 tatacttacg cattaattag aaaaatccta acgcattcta atctatctcg atatgacaag
      781 taaagtataa atttatgaat tgaatggttt tttttaaact ccatcgcaaa caatatcaca
      841 ttgcgatatt tttacttcac tcgatcacag aatacattgg ataatatgaa ctactattcg
      901 ggcagttcct caagtatcaa caatcttttg ttttaatacg cagtaaatca aacgaattaa
      961 atatgaaaac taaacccaaa agcaatcact cgcttgcctc agtcctcatg aagatgatct
     1021 taatcatgat gttagggtta ggggtatggt tagcctatga agtcttctat ctgcaacccg
     1081 cggataattg gtttgaagtc aaagcaaaaa ccagtagttc tgttaatctg cccaaagatg
     1141 atgcgccgca tcttgcaaaa atggaatggt ggtactataa cgggcattta tggactgaat
     1201 caggcaagca attcagcttt cactacacaa cctttatggt taccgagctt gtaagccatt
     1261 tggtcaaaca ggtttcgctt agtgatcacc agaccggtaa acactatacc gatcaaaaaa
     1321 gaacgacggg cgggggcttt tcctcaaata tcgaaaatgg gtttgagttc cttttagata
     1381 attggaaaat ggttggagat gatggcaatg atcaattaat tgtttcaaca ccccagttta
     1441 gctttaatct caaattaacc aatactatac ctcctgtctt tcatggcacc gatggtatta
     1501 ttttcttaga agaagcaggg aatagttatt actactcccg aaccagaatg gatatcaccg
     1561 ggacaatcaa aattggcaac actactgaaa ctgttcgggg tttatcttgg tttgatcatc
     1621 aatggggtga cttttcaacc aacctcttgt cttgggattg gtttagtcta cagctagata
     1681 atggaattga tttaatgatt tatcaactac gtgacaagta cgataaacca ctgctttata
     1741 ccggaagtat cacacagaat aatgttaccg aggctctgct tagtagtgac tttacgcttt
     1801 ctcagctggg tgatcagtgg actagcaaga aaacgggata cacctacccc attgcttggg
     1861 agatcaagat acccaaaaaa aatatccata tcaaaacccg gagcaaagta gaaaatagtg
     1921 aatttgatgc tcaactaaca acttataacc attactggga aggtgcggtg aagatagagg
     1981 gaacccatac gggtaaagga tttatggagc tatatggcta tacaaacaaa aaagaaccta
     2041 acgcctcgag atccaaaaag aggtagctca tgtttaccaa tatactcctt gtttgcgttg
     2101 gtaatatttg cagaagcccc atggctgaag ggttattaaa gcatcattta gctcaatcag
     2161 ctcaaacaaa tcattttgtg acttctgcag gactaaatgc gctagtcggg cacaaagcag
     2221 atgagaaagc ttgccatgtg ttaatacaaa aaggcattga tatttcttca caccgggcct
     2281 gccaactcaa tagtgtcatg gttcgcaaag ccgacctcat tttggttatg gagacatcac
     2341 ataaaaaggc tatagaggac aacgagcctg gcgctagagg aaaagttttt cggctgggag
     2401 aatggggaaa atttgaaata gcagatcctt accagaaaga cttatcaatc tttgaagatt
     2461 cagcattgct catcgagcag ggtattaatc aatggttaac aaaattgtaa tcagctgtat
     2521 atacattcaa cgcaagtttg acaaaaaagc cagaatagct taaacatgct ctcttgcgat
     2581 gtcggaaatt tttacatagt agacattttt ttatagggaa ctctatgcat ttaaaaattt
     2641 tcttagtcct tgcagcgtgc gcacttcccg cctgctcaat cgtaccagga caacacatga
     2701 gtcctttttc cagacaatcg agcgttaaaa tgccagtgac tgagaataat gagacaattt
     2761 taaaaacgct tgatattcaa accattaatg ctgagctcgt cttagcgtta gagcgtgatt
     2821 tcaatacacg cagccttggt cccgataatg tggccaatca ctattttgat taccgtatcg
     2881 gttcaagaac catcaaaggc gttcccatgg aaacagagcc ttatactcag taccgggttg
     2941 ggccccgcga tatccttaac atcacggtct gggatcatcc tgaactcacg atccctgcag
     3001 gtcagttccg agcagcggag aaagcaggca atgtcgttgg tgaagatgga actttttttt
     3061 atccctatgt aggtattgtg catgctgcgg gaagaacggt agaagaaatt cgggatgagt
     3121 taaccgagcg attatcaaga tatattgaaa atgttcaatt agatgtgcgt gtcgctgatt
     3181 accgtagcca gcgcgtatat gtcgtcggtg aagttgctca accgggcatt cagctagtta
     3241 aagatattcc actaactgtg ctcgaagcga tcaataatgc gggaggcgtc aagtcggaag
     3301 ccgatcagcg caacattacc ttaacgagag atgataaaac ttacagtatc aatttgttaa
     3361 gcttatatga agggggagat atcactcaga atgtgcttct aaagcatgga gatatcctca
     3421 atgtaccgga tagttcatta aataaagttt tcatattagg cgaaactaat tttagagtgc
     3481 ttggcggcgc cggtattgga cgctctagaa gcctggtcat gaataaagcg cgaatgacat
     3541 taaccgaagc actcagcgat gcaggtggat tcgatcaaga aacatccgat cctgccagaa
     3601 tatttgtatt tagaggcgga gtaggtaaat cagaaatttt tcacctcgat gccaaatcac
     3661 ccgacgcatt actgttagca gatcgtttcc cactacaacc gcgtgatgtc atttatgttg
     3721 atagggcaga aggtattcgt tggaaccaaa tcattgctca aatccaaccg actgtgaatt
     3781 tattgaattc gtttgatggt gtgttaagaa ttcaaccctt tcgagcattt ccgtcagcac
     3841 caagcacggt acgataacgg tctatcgatc attgaaccaa tgacaagcta gtcgttaacc
     3901 cgatcaagta agggaatggg aacgctatta tttactaaat atccatgcgc taaaaaaaca
     3961 ctttttctct actaacaaaa tactatgtcc aatttaccca ctgatcgttc acccactgca
     4021 ttcgattatt ctcttaacca agatgaagat gttatcaatt taggtgaact attcggtacg
     4081 ttatatgaga gtaaatggtt aattctattg ataatgacgg tcacagtttt aattggtttt
     4141 gctaaaacac agttagatag gccaatttat aaagcggatg gtctgctcca agtcaaagaa
     4201 aactcaacct cgatggcggg tttagagcct ttggccggtt tgatagaagg taaaatggct
     4261 gttatggctg agatcgaatt aatcaagtcg agaatgattt tgggtaaaac cataaaaagc
     4321 ttacatctcg atgttatagc caaacccaga tattttccaa ttgtaggaga aaccattgcc
     4381 cggcagtttc aaaatcgaaa tcaagatgga agcgtctcaa atccactctt tggactaacg
     4441 cagtatgctt ggggtgggga aacgattcaa gtagaaacgt tcattgttcc gtcaatttta
     4501 gaagatgaag aattcatttt aaaaacagat caggaaggct tcttcaaact attctataaa
     4561 gatgaaatca tcctggaggg agaagtcggg aagcttgcga ataagcaaat cgaaggctat
     4621 tcgcagccga ttgctatttt tgtttcattg ctgaaatcgc gacctgagac acactttacc
     4681 atcgtacgcc aatccgagaa ccaagcgatt cgccaactca aagaaaggat ctcgattgct
     4741 gaaaagggca aaaatactgg catgcttgaa ctaactgtgg agtcatccct acctgatgct
     4801 gcagttagcg ttttaaacga aattgccaat atttatgttc aacagaatgt cgaccaaaaa
     4861 tcagcggaat cacaaaaaac cctggagttc cttgaaaagc aattgccaat tctaaaagat
     4921 caattagatg ccgcgacaac gtcactaaat gaatatcgca cccgaaaagg gtctgtcgat
     4981 ttgaatctcg aaatgcaaaa tattcttaaa aactctgtcg aactcaaaac acaaactacc
     5041 attttgcagc agaaacgcga tgaactcagg caacggttta ctgaatcgca tcctcatatc
     5101 attgccatcg ataaacagat tgcaagatta caagggcaaa tcagcacgca agataaaaaa
     5161 atcgagactc tacctgaaac gcagcaggct attctgaaat tatccagaga tgtaaaagtc
     5221 agtaccgagt tatatacaac gctgctcaac aatgcccaaa cattgcgcat tgcaaaagcg
     5281 ggaaccattg gaaatgttag gattattgat tatgccattc taccggatga gccgattcgc
     5341 cctagaaaag tattgattat cggtattgcg ctcgttttag ggatcatctt tggtgttgta
     5401 accgcattaa ttcgtaaatc gctcaatcgt ggtattgaag atcctgattt aattgaaaaa
     5461 ctactgaata ccccagttta tgcaacgata ccgcatagta aattccaaaa agcactcagc
     5521 aacaaattga agcataatca agcaaataat gtagcttcac cggttattct tgcactcgag
     5581 aataaagaag acgttgccat tgaaagcttg cgcagcttaa gaacgacact gcattttgct
     5641 ttcctcgaag cacagaataa tatcattatg atcacgggac ccagtcccgg tattggtaaa
     5701 actttcgttt ccgtcaatct agccgtcgtt atggctgatg caggcaagaa actgctgctg
     5761 atcgatggtg atatgcgccg gggctatatc aacaaagtgc tcggtgtaaa tcgagaaaat
     5821 ggtttatctg agctcatttt tgaatcgatc agtttagata aagccattca tcccattgag
     5881 agtgctcatt ttgatttcat tgcaacaggc gccataccac ctaatccatc cgaattatta
     5941 ctgcatgagc gttttgcaaa tcttttagac aaccttagca aacaatatga tctagtcatc
     6001 attgattctc cgcctattct tgctgttaca gatgccgccg taatcggtcg tttggctggc
     6061 gccacactga tggtcatcaa agcagggcaa catcctgcaa gagaattgga gcaaagtacc
     6121 aaaagactga tccaagcggg tgtcaatcta aaaggcatcg tgttcaatga cttgccagaa
     6181 cttgcctcgc gtcctggcta tggatatggc aagtatgtct atcagtattc ttacaaaagt
     6241 ggcaaatagc gcatccacaa atttcgtcag gattttaaag actcgagatg tttttccaca
     6301 tcagctttgc taaatccaat tgcgctcgca acgcggtcag catggctgac tcgtgaaata
     6361 ccgggaatca atcgatgggt aggctcacct tgtaaaaatt ctacttgcag atagcttcca
     6421 attccttcac tgagtagccg ctcacagagt tcatgattat gtgtcactaa tagcgttgat
     6481 gctcccaatt gatgaaatcc ctttaaaaca tattcagaca acgtcatttt ctcttcaaat
     6541 gtcgtccctt cagccaattc atccagaata accaagctaa gtgatgtcga attgaaaaaa
     6601 atctcacggg tctgtttcaa ctcatgtgca aatcgaccca ttccttcttc tagtttccct
     6661 gcatcgggta tttgataaaa aatatgttcg gcagggacaa tccgcgccat ggatgcaggt
     6721 agataacttc ccgcttgccc taaaatctgc agttgcacga tcattttgca ataagcggtt
     6781 ttacccccgc tattagggcc ggtaataatc aacaatcgat tcgctgcatc caatttcacg
     6841 tcattagcca catagtgcgt attagtgttc agaagcaaag gatttttagc ttgtattgct
     6901 gttagcgtat gttgaccagc agtcataatc tcaggcaaga ccatttcatg atgtgatgcc
     6961 ttggcataac gatgcagcga taaaagctca tcgagcatac cgagtgcttc catcgccttt
     7021 gctaattccg agctttgtcg aaaaagtttc tgcaacgggt aaataaccga atcccgatct
     7081 gaagaactta ccgcctgaac aatgatcgga aaaattggca ctgtcaggat taaaataccg
     7141 tatccaatat aattggcacc cagctcaacc aacatgttct cgaagaaata aagtaaacca
     7201 ctcacaatca gaacgaaaat taagctgggt agccacttga acatcgacgg ccggaatcgc
     7261 ggaataggta aataaggcgg tttttcgtct ttagtcttaa acttaccatc gatgacataa
     7321 aaagggccat gcatcaaagc ataggttttt gaaccgccaa aatcacgaat cgcctgaagc
     7381 aattcctgca aataggcact ttgtaccggc ggcagctcac cagcttcttc caccaaatcg
     7441 acagcaaatt cagtcccatc gatgaattgt cgataaccat accccccaaa ttcaagttta
     7501 ccaacccgat tcttaggatg atctgtcgtg aatccaccag aaaactcacc atacagtaaa
     7561 tggtaaagcg tttcttctcc atcggccatt ttcttgatga actgttcgca agcgtctagt
     7621 aacgggggat tcatcgctaa ctctcgtaat gcttcctgtt ttttatgcag ttgctcaaca
     7681 tgatgtgtcg gttgagctaa tgaacgatac aatacggcct ggccgatttg agtctcagca
     7741 tgattcattc cttcaaatag tgcgtccact tcgacagcgt caaatgtttt cggatcaagc
     7801 acaccatatt ccgtttcagt gggtctcgtc ccctttatct cagcaggttg cttacgattt
     7861 gataagatat agggtgttct aaaaatagcg gttggggttg agtcagtcac ttctgtcata
     7921 aaccaccttc aaattatgta tataaattgc tatatgttat ttctcacagc gtttagctgc
     7981 aatataacga gaaataccac tcattaattg ctgacatgaa acaaaactta tttttaatac
     8041 tccatagtgc atagtatttt gagaaaacac acccgtgcct cgaaaatttt ttaaaaaata
     8101 tctcccttct cacgaaagca ttcaacagaa tcggttcatc agtttttttg gtgagctgtt
     8161 gcatcatcat aatctgtggc atctccatcg tcgatcggtg gcaggtggct ttgctgcggg
     8221 gttattcgca ggactcattc ccggtagtaa cccagttcaa ttcttctttg ccgctttatt
     8281 ctccatcacc tttaaagtga atttgccagt tgctgctttg acaacactct attcaaatcc
     8341 atttacgatt ctaccgctct attggaccgc gtacttactg ggtggtttct ttatcgataa
     8401 tggcatgaat aatcttccgc cattggaact caatttagct gacaaatctt tttctgaatg
     8461 gatgccggct gtcgtgactt ggctcacctc gtttggaaag ccgttgatca tcggattggt
     8521 cttacttgca tcattgcttg ccatcatcgg ttattttttg gtccgactga tttggcggtt
     8581 ctatgttatt catgcctggc ataagcgcca ccagcgcccc caaccctaga cattttgatt
     8641 taatctaaaa tatcaattat taaacgggct acatgcttag gccttacatc actcatcatg
     8701 cagaaaaata aaattatcag tcgtttactt atcattcttg ttaccatcat ggtcgcaggc
     8761 tatatcggtt ggcacctgtc gcgtaaaccg ccagtcgaag tagagttagc tactgctagt
     8821 catggcactg tggaagcgat tgtcgtcaat actcgggcag gaacgattaa agcttgccga
     8881 cgagcaaaac tcgcaccggc tgcaggcggg cagatcgtta aacttttagt aaaagaagga
     8941 gaacgggttg tccagggaca acgattactg gagctgtgga atgctgatct tgctgcgcaa
     9001 catgagcttg cagcgcaaca actggcaaca accaaaagcc gtcaacggga aaattgtatt
     9061 atcgccaata acgcacaacg agaatccata agaacccagc aactcgttgc aaaagggttt
     9121 gtcagttcac aacgtgccga agatgctgaa gccaatgcac aagcacaaca agccagttgt
     9181 gccgctgcag gtgcagatgt aaaacgcgct caagcacaac tcgaggtaac ccaagccaat
     9241 ctgaaccgta ccctactcaa cgcaccgttc tcgggtgtca ttgctcaagt aactggggaa
     9301 ctcggtgaat atacaacccc ctctcctcct ggcattccaa caccacctgc aatcgactta
     9361 atcgatgatt cctgcctcta tgtcagcgcc ccaatggatg aaatcgatgc gcccaaactc
     9421 gccattgggc aaaaggcacg catcacgctc gatgcattaa ctaatcaaac atttgcgggt
     9481 gtagtccggc gtattgcccc ttatgtaaca gaaattgaaa aacaagccag aaccgtggat
     9541 gttgaagtca atttcgtaga aaattcaaac caagtgttgt tagtcggtta cagtgcagat
     9601 gttgaggtca tcactgacca gcgcgagcaa gtattacgaa ttccaactca agcgattcgg
     9661 cagcgcaata aagtatgggc actcggtcct caggatcggc ttgaagaacg caccctggaa
     9721 attgggcttt caaactgggc ctatacagaa atcatcagcg gcttgcaagc aggtgatcaa
     9781 gtattaatcg caaccgatat cgaaacgatt gttcccggca ttcaagtaaa acctaaaggt
     9841 ccaccatcgt gattgaactc ttaggcatct gtcgtacctt tcagatgggc gatcaagcaa
     9901 ttcatgcgct gaatcagatt aatctaaaaa ttactgctgg cgaatatgtt tccattatgg
     9961 gaccttccgg ctcaggaaaa tcgacattgc tcaatgtgat tggattactc gatcaaccca
    10021 atagcggacg atatctttta gatggtagcg atgttactca tttatccgaa tcagaacagg
    10081 cccgcgtgcg ccgagataaa attggttttg tatttcaatc attccacctc atcccccgcc
    10141 tgaccgccgc tgaaaatatc gagctgccgc tgatcttatc gggtattact cccagtgagc
    10201 gtaagcagcg cgttgaagct gcattgctgg catttaactt agttgagcgt gcgcaacatc
    10261 gtcccgcaga gttatcgggc gggcaacggc agcgtgttgc aattgcccgt gctacagttc
    10321 tgcgccctac tgcattattg gcagatgaac caaccggcaa tctcgatcac cgcattggat
    10381 cagaagtcat cgccctgctg gaaaatcttc accatagcgg aacaacactg atcatcgtaa
    10441 cgcatgatcg tgagcttgga ttacgtgccc atcgtcgtat cgcgatgcgc gatggcagca
    10501 tcatcgctga cgaacaatga aactctccga tatcctacaa ttttcattga atacgctaag
    10561 aagctacccg attcgctcgt ttctcatcgt tctcgccatg gctttgggcg tagcagctgt
    10621 cgtcgtccta actgccctag gggacggtgc acgacgctat gtcatccaac aattttcatc
    10681 gattggaacc aatctaattg tcgttctacc tggtcgagct gaaacagcag gaagttttcc
    10741 tggtgccgct atgggacaaa cgccgcgtga gctgaccttg gaagatgctc gttggttagg
    10801 atacataccg caaatccggc gctatgcacc gctcaatgtt ggcgctgcgg aacttgcagg
    10861 tgccggtcgg ctacgcgaag ttaccgtatt gggcagcacg gtcgatttac taccgattcg
    10921 ccatatggag cttgctcaag gggggttcct caatcaaggt tctgaaaata atgcacaaat
    10981 tgtattgggt gctaagcttg ccaaagaatt cttcccagat ggtaatgcag tgggtcaaag
    11041 agttcggtta gccgatcggc gatttatcat ttctggtgta ttagccagtc agggtgaaac
    11101 catgggcttt aattctgacg aaattgtaat catcccgatt tatcatgccc aagctctgtt
    11161 caacaccact tcactgtttc gaattttgat tgaagtcagg aatcgcagcg atattccaac
    11221 caccaagcaa gcgatacaag aaatgctaat tcaacgccat gatggcgaag acgatgtaac
    11281 ggttattacc caagacgcag tgctatcaac ctttgatcgt atccttaatg ccttgacact
    11341 cggtgttgct ggcattgctg ctatcagttt agctgttgca gggatactcg tcatgaatgt
    11401 catgctggtt tcagtcagtc aacgaacggc tgaaattggc ctatttaaag cgattggtgc
    11461 tcaatcccat gatatccgct atctgtttct agctgaagcc acttggctat cgctggttgg
    11521 ggctatcatc ggttttatat taggccaatt cggtagccta ttaatacgaa ttgcttaccc
    11581 acttctgcca gcttggccac caatctgggc ttgtattgca ggtattgttg tcgccattag
    11641 cactggcatc attgcaggta ttttaccagc cgttcgcgca tcgcgccttg acccggtgca
    11701 agcgctaagt agcaaatgaa tttcatcgaa tcacttcagt ttgccctcca tgcgctatca
    11761 gcccatcgcg gtcgcacgat cttatccgca tccgggatcg cgattggtat cgctgctgtt
    11821 ttactactga cttccattgg cgaaggtatt cagcgctttg taatttccga atttacacaa
    11881 tttggcacca atattctcaa cattacgcca ggaaaaattc gcgcacatgg tggttctgtc
    11941 ggttcgattg gtagcgcacg tatcctcacg atcgaagatg cgcttgcgtt gaaaacatcg
    12001 cgctatgtcc aatatatcaa tgcaacggta tttggtaacg cagaaatccg tgcggaaggg
    12061 cttagtcgcc gagtaactgt ttatggacaa ggtcccgatt ttgataaagc gtttaatatg
    12121 aatgtagcga tcggtcagta tttaccgacc gacgatcccc gtaaccctcg cgcctttgct
    12181 gtgctcggcg ctaaggttca tcaggaatta tttggaacca tgagtccgtt aggtgccatt
    12241 ctgcaggtcg gcagctcacg ctttcgcgta atcggtgtca tggctgcaaa aggacaagtg
    12301 cttggctttg atttggacga taccgtgttt atccctacgg ctcgtgcact ggaagtcttc
    12361 aatcgcgaag gtgtgatgga aatccaagca gtttaccctc cggaatttcc tgttaacctc
    12421 gttaccgaag atttacaacg tatcctcatc gaacggcatg ggagggaaga tttcaccatt
    12481 accccgcaaa aacaaatgtt aagtacgctt tctaccgtct tgaaagtact cacgtttgca
    12541 gtcgctgcac tcggtggcat ttccttgctt gtgggcgcag tgggtatggt caccctcatg
    12601 catattacgg tgacagaacg tgttgctgaa attggtttac tcaatgcact cggtgccaca
    12661 cgagcccgta ttagaacgtt gtttctcctg gaatcgacca ttctttcaac aatcggtggc
    12721 ctagcaggtc ttattattgg cgcgagtatt gcttttttgc ttaaaatctt cgtcagcgct
    12781 ttaccagtca acattccctg gcagtttgtg agcatcgcac ttggtatctc caccttgatc
    12841 ggactcattg ccggtgtctt tccagcgatt caagcagcac gactaaatcc aatcgatgcg
    12901 ctgaggagcg agtaattcac actgatcagc gatgatttca acttgaacaa tgagattatc
    12961 gctacaaaaa aatcacatta ttcttccccc attctccaac cattctcggc aatcgggagt
    13021 caatgctcgc tgacaagctt gatcataaag cagcaattct ggctccgata gcacatcgcg
    13081 ccagcggcca ttactccctt tatggagaaa agtttgtgcg ccacctttcc agaaatgccc
    13141 accaccaggt gcatatttct ccccttcacg tttcatctca ttgaaggaaa ctgctttcac
    13201 aatccgtggc caagtatcct cgggtacttc aatctcaagg aactcagcca ttctgcgcat
    13261 ttcacgctcg gtattttcca acatatcgct ataatgaaat aactgaatat tgggtaaatg
    13321 ccgaaaattc caccacgact gcacattgga aagatgagac cagtaaggcc agccatcgtt
    13381 ttcccaagca aaaccaccgc gggttatcca attttgccag aaggtatgaa tgttctccgg
    13441 tggatgcggt aattcttctc caactcttcc gggcaagcca ttcattagtg caaaaatttc
    13501 ttctttcata cccgtgtagt gattccatag tgacatgaat acatcccttg tatcccgggc
    13561 gatatagaga tatttaaagt gcggatggta aatcattcca tccaaaggca agtgtgtttt
    13621 gataaagcgc cgatgattct gggctttgag tcgattcaag ataacttcga gtggcatgat
    13681 acgcatatcg agccaggggg acatctcggc cggtggtgca ggaaaattcc catcctgaaa
    13741 aagtaagtgg gccacaatgg cttgcgtcca ggttgttcct gctttataag acgttgccac
    13801 aataatatca gtggaccgtg gctcaaaata atcccagcgc gtactgtcga aatgatgatt
    13861 ttgataaatc cggttacgtt caggtaattt caacatagcg ctaccttttt tgttagggtt
    13921 agaaagagaa aataagttag cattttttta atcactcgcg tttgattggg aaaccgattt
    13981 gctgcagttg cgcggtttga taagcaagtg cagatagagc aagatcgcct agagcaagtc
    14041 cacggaaaat aaatgctgtt ttttgctcat cggtactgcg acctttgatc acatcgcaaa
    14101 cgagttcagt taaatctcca gtgatccaat gtggattaat taatggtgtt tccatcactg
    14161 cctcttgttc acgatcatca atcacaatgt aatccaactc tgaaaagctt tgcttgatcc
    14221 aaggcgcagc cagatcagtc actgtagcaa aggcacccgg ttttagccat cgcgcatcga
    14281 gaaaagggac taattg
//
LOCUS       DAOQLJ010000049        14155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1581_length_14155_cov_6.117376, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000049 DAOQLJ010000000
VERSION     DAOQLJ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 14155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14155
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1581_length_14155_cov_6.117376"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PK522_05095"
     CDS             <1..245
                     /locus_tag="PK522_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41142.1"
                     /translation="SMPLDDVLDLCVQGRFIEASYSPSKRRWFIPIPVRMSRAMIKRC
                     YKRKPFKPIDYERFKETRLLSRSLSRRQNFSLVFFY"
     gene            complement(247..606)
                     /locus_tag="PK522_05100"
     CDS             complement(247..606)
                     /locus_tag="PK522_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNC41143.1"
                     /translation="MVSTNPILISITLLIISNIFMTFAWYAHLKELNSSPWFIAALIS
                     WLVAFFEYLFQVPANRIGYTVLSVGHLKIMQEVITLSIFVPFAIFYLKEPLKLDYLWA
                     GLCMVGAVYFMFRDKIN"
     gene            complement(613..1998)
                     /gene="fumC"
                     /locus_tag="PK522_05105"
     CDS             complement(613..1998)
                     /gene="fumC"
                     /locus_tag="PK522_05105"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNC41144.1"
                     /translation="MSNFRYEHDSMGEIQVSEGVLWGAQTQRSLLNFKISTEKMPFHL
                     IQALALVKRAAAKVNLELGLLDKTRAEAIISAANEIIAGQHSAEFPLAVWQTGSGTQT
                     NMNVNEVIANRASEILGGGRGNLRNIHPNDDVNKAQSSNDVFPTAMHIAAVQAVCNHL
                     MPALALLRNTLALKAESFKHIVKIGRTHLQDATPLTLGQEFSGYVAQLDHGMAHLKAA
                     LPHLYEIALGGTAVGTGLNTHPEFAHRVAKEIAGLTHFPFISAPNKFEALAANDALVH
                     AHGALKTLAASLMKIANDIRWLASGPRCGIGEIKIPENEPGSSIMPGKVNPTQSEAMT
                     MLCCQVMGNDVAINMGGALGNFELNVMKPLIIHNFLQSVRLLADGCISFHEHCVNGIV
                     ADTNRIDQLMRNSLMLVTALNPHIGYDKAAEIAKKAYHDNSSLKEAAVALGYLSAEQF
                     DNWVIPEKMVG"
     gene            complement(2037..2453)
                     /gene="queF"
                     /locus_tag="PK522_05110"
     CDS             complement(2037..2453)
                     /gene="queF"
                     /locus_tag="PK522_05110"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNC41145.1"
                     /translation="MLNKPSKKLEAFDNPFPNRDYRIHMEIPEFTCLCPKTGQPDFAR
                     LILDYIPDKKCVELKSLKLYVWSFRNEGVFHEAVTNQILDDLIALLKPRFIRITAKFF
                     VRGGIFTNVVVEHRKKNWKPVIPVILEAITEQSNTR"
     gene            2548..6117
                     /gene="smc"
                     /locus_tag="PK522_05115"
     CDS             2548..6117
                     /gene="smc"
                     /locus_tag="PK522_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNC41146.1"
                     /translation="MRLFQIKLAGFKTFVEPTTITIPGKLVGIVGPNGCGKSNIIDAI
                     RWVLGESRAGTLRGDSIQDVIFSGSVHRKPVGRASVELVFDNSLSKITNQWSSYAEIS
                     IKRVVQRDGDSAYYINNTHVRRRDIIDLFLGTGVSSRGYAIIEQGMISRIVEAKPQEL
                     RSFLEEAAGIAKYRERRHETELRLIDASANLLRIKDILQELEKQLLHVKEQAAAAQRF
                     NALQTELRQINSLLWLHSQQEAINLRTRTTAEIEQLMLKLEQETNNWQESDKNLEALR
                     VQHAAISDDHQRAQALLYAIEARLARTEQELRHIEENKQAIQLQIDEANTQLHRNQSN
                     KEQFQTELTHLSLEREKLDNLQKAHQQQHETENEKLPEFDHRFRISQDELFQIKQKHA
                     VTQEASKLFLNQLTHSEKIINQLTQKQTRLLQEKNNQIDSQQDKIYVLQTELEQLTFE
                     IESKQTSLTEIEEQLNLLEQSRKETLHTVQQVQQRLTSAKARYDALAQLQRQLENNEA
                     LTNWIIHNQLDQLPRLWLHIEIEPGWENALESILQEHLNAALVNRLDIALNWAEPAPG
                     PWCIVEECLNDKIKDNNALSIEFTNTSSKNNYPYKKPLSSLVKFTNSRIEEIVKQLLL
                     DVFTIDDIETALRFRHELAPTERFITSQGHLISQNSLFYYAPHTQYHGVLQRQREIVQ
                     FKNLIDQYQKEFLDIDEIYAEIEKSNEYCKQSRSTLQHELAQIKQAKHDLSLELIKLS
                     EFTQRIRDRNQQIDEELIDISAELETEFIQKQTIESKLAAHQSEIDQLSLQIDQLNES
                     VESAEKILITHRQLVHNLSNQWRDTSVQFKDCQNRIVQTENNINNLDQSAETYRNNIE
                     KLSQSLCLLENQDLQLHLAEQTDERHHCELSVTKFRQVLEETVQIIRNSELARMKSEQ
                     AINQYKESLNQLRLKQQEACLIENQFKEKLMESGSDVSTLLPQIEKVKPSVLKAELNR
                     INAELTSLGLVNLAAIEELQTIQSRFTFLDLQSADLENAVMTLENAIHQIDGETRERL
                     QETFDRVNLLFHELFTSVFGGGNAKLTLSGEEILDATIQLIAQPPGKKNNSIHLLSGG
                     EKALTALTLIFSLFQLNPAPFCLLDEVDAPLDDINAMRFCELVKRMANETQFLFISHN
                     KISMQMAEQLIGVTMAEQGVSRIVSVNFEDVINPVQDQEARLV"
     gene            6204..7331
                     /locus_tag="PK522_05120"
     CDS             6204..7331
                     /locus_tag="PK522_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HNC41147.1"
                     /translation="MSDLQISLIIIGAIIIAGVAIFNWVQQIQYQRNIKKAFEHDHED
                     ALLKPSKFVSKEERIEPKFNQQANESELEPIVPIATEDIPTIPAHISVTPTHEQKPIH
                     IDSVINYITSITTTDLITHDKLTELLQQKFDFGKPVTWYGLNRDLQAWEEITIETLHG
                     RGFYSQLKGCLQLADRAGPVSEVNLSKFRDMVEDFASQLNVIAECPDIAQSHTNAITL
                     DKFCADVDVVLGINVVSKDGGAFVGTKIRAMAEASGFKLESEGMFRFRDENNATLFSL
                     CNYETTPFSPANMRTLTTHGITFLFDVPRVANGEKVFEQMAHLAKLFASTLNGIMVDD
                     NRVPLSDNGLKRSKQQLKNIQAAMSAHNILAGSDVALKLFV"
     gene            7362..7595
                     /locus_tag="PK522_05125"
     CDS             7362..7595
                     /locus_tag="PK522_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNC41148.1"
                     /translation="MSEIKKFSPIEVTLEANRQYYWCSCGLSKSQPFCDGSHKGTTFT
                     PLAFSVEQTKSVWLCTCKKTANKPYCDGTHNKI"
     gene            7629..8624
                     /gene="lpxK"
                     /locus_tag="PK522_05130"
                     /pseudo
     CDS             7629..8624
                     /gene="lpxK"
                     /locus_tag="PK522_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
     gene            8649..8828
                     /locus_tag="PK522_05135"
     CDS             8649..8828
                     /locus_tag="PK522_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNC41149.1"
                     /translation="MDTKLLDILVCPLCKGSLIYKKVEKELICKRDRMAFPIRDGIPI
                     MLEDEARLLPPEEEV"
     gene            complement(8934..9104)
                     /locus_tag="PK522_05140"
     CDS             complement(8934..9104)
                     /locus_tag="PK522_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41150.1"
                     /translation="MRPQIITQLSDFPESIDEQIPDTRVMKVIDDTDFTDDDFEVEEI
                     EVNKVINDLLLH"
     gene            9435..10265
                     /locus_tag="PK522_05145"
     CDS             9435..10265
                     /locus_tag="PK522_05145"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HNC41151.1"
                     /translation="MNDQQLKILIKLNQIPQSPVSTIADNSEITESHDEEKLPFDWLK
                     ISAALLLLFLLLGVSYWLLTGSEKESAESSIFSNEDRSQSTDSNQQAPSDLESIVDAT
                     SPETARTIVVPSDLGKQTESVSNAVTSNENHAQQAMIIPSSKPESKGLKLNNSQSKEK
                     PTQTQSNYVIKAQLTTSIRKQESINSIDHIELQRGMNQTIYYSLHIRNLKGEKIFVNW
                     YYQDKQVAKIPFSINTNDWQAHSSKILTKTSLGRWRVTAMDQLGNILSEQFFLVSNRS
                     "
     gene            10313..10855
                     /locus_tag="PK522_05150"
     CDS             10313..10855
                     /locus_tag="PK522_05150"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNC41152.1"
                     /translation="MNYCSHCSAPVELRIPDGDSLPRFVCGTCNTIHYQNPKIIVGCI
                     PEWNNQILLCRRAIEPKIGLWTIPAGFMENQETLAEAAIRETNEEANANIEIGDLYAV
                     YSLPHISQVYVLYRAKLIDTNFFPGIESQETKLFHEHEIPWGDIAFKVIHDSLKRFIE
                     ERRQNSIQFYTGVITRAPSQ"
     gene            11156..13039
                     /locus_tag="PK522_05155"
     CDS             11156..13039
                     /locus_tag="PK522_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNC41153.1"
                     /translation="MSLEQLMNVSITGASKYEQKQSEVAAAVTVITRNDIRAYGWRTL
                     SEILTSLPGVFATYDFSYNYIGVRGFNVLGDFNTRILVSINGNRINDATYDQGPMGRD
                     FPLDVDLIERIEFIPGPGSAVYGQNAMLGVVNIITRKGSNVNGVELSGSYQTAEVMPQ
                     ERATLGKKFDNGVDALVSVSGVQARGENLFFDFRDAAISGVAHHMDGENIKQIFARAE
                     KGPVSFDFIYGNRKKDDPTAIYFSDPLISGQYLKDRRLNSQLQYNDNFVNDTLNVLGR
                     LFIGKYDYDSPLVYEGKKTLSTGPSFWHGAELRLVSSAITNHKLMAGVEYQNNTHIKQ
                     TFEYLESLADNITVRSSVVRTGVYLQDEWRITDTLSSTLGVRYDYNNWIGNRLSPRGA
                     LIWQATPKAAFKAIYGRAHRSPNSYERDYGDGVSQISNPGLRGETIDAAELVADYFPE
                     PNLNLRASLYAWDVYNIIALGFNPLLEASQYQQTHDKVRARGVELYFDKTWNWGARLR
                     SSFGIQDASQKGSHIPNSPQLLGKINVLVPIPLMSGLKGGYELQYFGERKTLNGPNTD
                     SYFLSNLNLMTDIRWVKGLEASLAVYNLFNEHYLHPVSDSSWHNSLLQPGRTVRFRLE
                     YRF"
     gene            13213..13707
                     /locus_tag="PK522_05160"
     CDS             13213..13707
                     /locus_tag="PK522_05160"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNC41154.1"
                     /translation="MLYSPNSFGQPEEPTEYRLKAAFLYNFASFPEPPTKIDKHSTFN
                     MCFLDDNPFDPYRDYITQRKVKDLQVTILLKSDTDKLSECHLLYISRSVASNKLESIL
                     DRISSGPILTVADSTGICQKEVVTNMGINAGKTTFKTNLTPAHTAKLELSSQLLRFAK
                     EAYQ"
     gene            13707..>14155
                     /locus_tag="PK522_05165"
     CDS             13707..>14155
                     /locus_tag="PK522_05165"
                     /inference="COORDINATES: protein motif:HMM:NF028461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HNC41155.1"
                     /translation="MNPLLPATTTIKNKLQKISLISQSTAMFLLAGLVILSSFIINLY
                     SLLETSQSTAKILAENATATLMFQDKNSAQTLLYSLNNLSRVNAAAIFSENHHQFAEY
                     PNNHHAFPHFDSLNEEKVLIDAQYIKLLQPIRFNDQSLGALYLEVTM"
BASE COUNT         4542 a         2814 c         2652 g         4147 t
ORIGIN      
        1 acagcatgcc gcttgacgat gttttagatt tgtgtgttca aggtcgtttc attgaagctt
       61 cttacagccc ttcaaagcgt cgttggttta tcccgattcc cgtgaggatg tcgagagcca
      121 tgattaaacg ttgctacaaa cgcaagccat ttaaaccaat cgattacgaa cgattcaaag
      181 aaacccgctt gctttcaaga agcctaagta ggcggcaaaa cttcagcctt gttttttttt
      241 attaatctaa ttaatcttat ctctaaacat gaaataaacc gcaccaacca tgcacagtcc
      301 cgcccataaa taatctaact tgagcggttc tttgagatag aaaatagcaa atggtacaaa
      361 aatactgagc gtgatgactt cttgcataat tttcaaatgt ccgacactta aaacggtata
      421 accgattcga tttgctggca cttggaaaag atactcaaaa aaagcgacca gccaactgat
      481 taaagccgca ataaaccacg gtgatgaatt gagctctttg agatgcgcat accaagcaaa
      541 agtcatgaaa atattgctaa taatcaataa ggtaatactg attaatatag gattggtact
      601 aaccatagct gattaaccga ccattttttc aggaataacc caattgtcga attgttcagc
      661 agacaaataa cccagtgcta ccgccgcctc ttttagagaa ctattgtcgt gataagcttt
      721 ttttgcgatt tcggctgctt tgtcatagcc gatatgaggg ttcaatgctg tcactaacat
      781 gagcgagttt cgcattaatt gatcaatgcg gttagtatca gcaacaattc catttacaca
      841 gtgttcatga aagctaatac agccatccgc taacaaccgt acactttgta gaaaattatg
      901 gataatcagc ggtttcatta cattcagctc gaagtttcct aacgccccac ccatattaat
      961 cgcgacgtca ttacccatca cttggcaaca cagcatcgtc attgcttcag attgagttgg
     1021 attgactttt cctggcatga tggagctacc tggctcattt tcaggaattt taatttcacc
     1081 aattccacaa cgaggaccag aagctaacca gcggatatca ttggcgattt tcatcaagga
     1141 tgcggctagc gtttttaaag caccgtgtgc atgcacgagt gcatcgtttg cagctagcgc
     1201 ttcaaattta ttaggcgctg atataaaagg aaaatgagtc agacctgcta tctctttagc
     1261 aactcggtga gcaaattcag gatgcgtatt taatccggtt cccactgcgg ttccacccag
     1321 tgcaatttca tataaatgcg gtaaagctgc ttttaaatgc gccattccat gatctaattg
     1381 tgctacatat cctgaaaatt cttggcctag cgtcaacggt gttgcatctt gcagatgtgt
     1441 acgtccgatt ttgacgatat gtttaaatga ttcagctttt aaagccaacg tattacgaag
     1501 cagcgctaac gccggcatta agtgattaca aactgcctga acagccgcaa tatgcatcgc
     1561 agttggaaat acatcgtttg aagattgagc tttattaaca tcatcgttgg gatgaatgtt
     1621 ccgtaaattt ccacgccctc ctcctaatat ttcagaagcg cgattggcaa tcacctcatt
     1681 cacattcata ttagtttgtg tgccggagcc ggtttgccaa actgctaatg gaaattcagc
     1741 actatgctga cccgcaatga tctcatttgc tgctgaaata attgcctctg cccgtgtttt
     1801 atcaagcaat cccagttcaa gatttacttt agctgctgct cgcttgacca aggccaacgc
     1861 ttgaatcaag tggaaaggca ttttttcagt agatattttg aaattgagga gtgaacgttg
     1921 tgtttgtgct ccccataaca caccttctga tacttgaatt tcacccatcg aatcatgttc
     1981 atatctgaaa ttactcataa ataaatcgac cgttgagtta aagtaatgta aacgttctag
     2041 cgtgtattcg attgttcggt aatcgcttct aatataacgg gtattactgg tttccagttt
     2101 tttttgcggt gttctacgac tacattggta aaaattccac cccgaacaaa aaactttgca
     2161 gtaatccgaa taaaccgtgg ttttagaagc gcgatgaggt catcgagaat ttgattcgta
     2221 acggcttcat gaaatacacc ttcattacga aaagaccaaa catacaattt taaacttttt
     2281 aactcgacac attttttatc gggaatatag tccaatatca aacgcgcaaa gtcaggctgt
     2341 cccgttttag gacaaaggca cgtaaattca ggtatctcca tatgaatacg ataatcccga
     2401 tttggaaatg gattatcaaa agcttccaat tttttggagg gtttattcag catgcgttac
     2461 attccatcta ggattgatta agtgaattca gataaaatag attaaaacga tttagttctg
     2521 aattatacca attatttacc ctctcaattg cgcctcttcc aaatcaaact ggctggtttt
     2581 aaaacgtttg tcgaacccac aacgatcacg atcccaggca aattagttgg tatcgttgga
     2641 ccgaatgggt gtggaaaatc caatatcatc gatgcgatcc gctgggtttt aggtgaatca
     2701 cgtgctggaa ctttgcgggg tgattcaatc caagatgtga ttttcagcgg ttccgtccac
     2761 cgaaaaccag tcggtcgggc tagtgtcgaa ttggttttcg ataactcctt aagcaaaatc
     2821 acaaatcaat ggtcgtctta tgcagaaatt tccattaagc gtgtcgtgca aagagatggc
     2881 gattccgcct actacatcaa taacacccat gtaagacgtc gcgacattat tgaccttttc
     2941 ttgggaacgg gtgtgagcag tcgcggctat gccattattg agcaagggat gatttcccgc
     3001 attgtggaag ctaaacctca agaattacga tcatttctag aagaagctgc cggtatcgca
     3061 aaatatcgcg aacgtcgcca tgaaaccgag ctacgtttga tcgatgcaag cgctaacttg
     3121 ctgcgtataa aggacatttt acaggagctt gagaagcagc tacttcatgt taaagaacaa
     3181 gctgctgctg cccaacgttt taatgcgctg caaacagaat tacgtcaaat taacagtttg
     3241 ttatggcttc atagtcaaca agaagcaatc aatttacgca ctagaactac agccgaaatt
     3301 gagcaattaa tgcttaagct tgaacaagag accaataact ggcaggaatc cgacaaaaac
     3361 cttgaagcgt tacgggttca acacgctgct atcagcgatg accatcaacg cgcgcaagct
     3421 ttgctatatg ccatcgaagc aaggcttgct cgaactgaac aagaactcag acatatcgag
     3481 gagaataaac aagcgatcca attgcaaatt gacgaagcta atacgcaact gcatcgtaat
     3541 caatctaata aagaacaatt tcagactgaa ttaacgcact tatcgcttga aagagaaaaa
     3601 cttgataatt tgcaaaaagc ccaccagcag cagcacgaaa cagaaaatga aaaattacct
     3661 gagtttgatc atcgattccg aatctcgcaa gatgaattat ttcagataaa acagaaacat
     3721 gctgttaccc aagaagctag caagcttttt ctgaatcaat taacgcatag tgaaaaaatt
     3781 attaatcagc taacacaaaa gcaaacgcgg ttgcttcaag aaaaaaacaa ccaaattgat
     3841 agtcagcaag ataaaattta tgtgttgcaa accgagctag agcaactcac gtttgagatt
     3901 gaatcaaaac aaaccagttt gaccgaaatt gaggagcagc taaatctctt agagcagtct
     3961 agaaaagaaa cacttcacac agtgcaacaa gtacaacaac gattgacatc ggcaaaagca
     4021 cgttacgatg cattagctca attacagcgc caacttgaaa ataatgaggc gctaacaaac
     4081 tggataattc ataatcaact cgatcaatta ccgcgattat ggctgcacat cgaaatcgaa
     4141 ccgggatggg aaaatgcatt agaatcgatt ttacaagaac atctcaatgc cgctttggta
     4201 aatcgcttag atattgcact aaactgggct gagcctgctc caggcccatg gtgcatagta
     4261 gaagaatgtt tgaatgataa gattaaagac aataacgctt tatcgattga atttacaaac
     4321 acttctagca aaaacaacta tccgtacaag aaaccgttga gttcgcttgt taaatttaca
     4381 aactcacgta tagaagaaat cgttaaacaa ttactattgg atgtttttac aatcgatgac
     4441 attgaaacgg ctttgcgttt tcgccatgaa ttggcgccaa ctgagcgatt tattacgtct
     4501 caggggcatt taatttcaca aaatagttta ttttattacg cacctcatac tcagtatcat
     4561 ggtgttttgc aacgccaacg cgagatcgtt caatttaaaa atttaattga ccaatatcaa
     4621 aaagaatttc tcgatataga tgaaatctac gctgaaatag aaaaaagtaa tgaatattgc
     4681 aaacaatcgc gttcaacact gcaacatgag ttagctcaaa taaagcaagc gaaacatgat
     4741 ctatccctgg aattgataaa gctatcagaa tttacgcagc gtattcgcga tcgaaatcaa
     4801 caaatcgatg aagaattgat tgatatttca gctgaattag agactgagtt tatacaaaag
     4861 caaacaatag aatcaaagct agcagcacat caatccgaga ttgaccaatt gagtttacaa
     4921 atagaccaac tcaatgaatc agttgaatca gcggaaaaaa ttttgatcac gcatcgtcag
     4981 ttagttcaca atttatcaaa tcaatggcga gatacttcag ttcaattcaa ggactgccaa
     5041 aatcgaattg ttcaaactga aaataatatc aacaatcttg atcaatccgc tgaaacatac
     5101 cggaataata tcgaaaaact aagtcaatcg ttatgtttac ttgagaacca agatctacaa
     5161 cttcacctcg ctgagcaaac cgatgagcgc catcattgtg agttatcagt tactaagttc
     5221 cgtcaagttc ttgaagaaac cgttcaaatt atacgcaact ccgaattagc tcggatgaaa
     5281 tcagagcaag ctatcaacca atataaggaa tcactgaatc aattaaggct caaacagcaa
     5341 gaagcgtgtt taattgaaaa tcaatttaaa gaaaaattaa tggaaagcgg ttcggatgtc
     5401 agtacacttc tgcctcaaat agaaaaagtt aaaccatcag tattgaaggc tgaactcaat
     5461 agaattaatg ctgagctgac atccttagga cttgtgaacc ttgcagcaat cgaagaatta
     5521 caaacgattc aaagtcgttt cacatttctt gatctacaat ctgcagattt agaaaatgcg
     5581 gtaatgactt tggagaacgc aatccatcaa attgatggag aaactcgaga acgcttacaa
     5641 gaaacatttg atcgcgtaaa cttgttattt catgaattat ttacttcggt ttttggtgga
     5701 ggtaatgcta aattaacgtt aagtggtgaa gaaattcttg atgctacgat ccaattaatc
     5761 gcgcaacctc ccggcaaaaa aaataactca attcatctct tgtctggtgg cgaaaaagcg
     5821 ttaacagcgc ttacgctcat tttctcatta tttcaactga acccggcacc attttgcctt
     5881 ttggatgaag tcgatgcgcc attggatgat attaatgcaa tgcgtttctg tgagctagta
     5941 aagcgcatgg ccaatgaaac acaattctta tttattagcc acaataagat ttccatgcaa
     6001 atggcagaac agcttattgg cgttaccatg gcagaacaag gggtttctcg tatcgtctcc
     6061 gtgaatttcg aggatgtgat aaatcctgta caagatcagg aagcaagact tgtctaaaac
     6121 ttactatcag attacttatt agtggctata attggtattt aaaaaagctt aatcaagttt
     6181 aataatattg aatggatcaa agtatgagtg atttacaaat tagcctaatc attattggcg
     6241 cgattattat tgccggcgtt gctattttta attgggtaca acagatccaa tatcaacgaa
     6301 atattaaaaa agcatttgaa catgatcacg aagatgcgct actaaagccg agtaaatttg
     6361 tatcgaaaga agaacggatc gaaccaaaat tcaaccagca agcgaatgag tctgagttag
     6421 agccaattgt accgattgca actgaagata ttcctacaat tcctgcacat atttctgtca
     6481 caccaacaca tgaacagaaa ccaattcata ttgatagtgt gatcaattac atcacatcaa
     6541 ttaccacaac tgatttgatt actcacgata aattaactga gttactccaa caaaaatttg
     6601 attttggtaa acccgtaaca tggtacggtc tgaatcgtga cttacaagcg tgggaagaaa
     6661 ttaccattga aacccttcat ggcagaggat tttattctca attaaaagga tgcctccagc
     6721 ttgcagaccg ggcaggccct gtgagcgagg tcaatttatc caaatttcgc gatatggttg
     6781 aagattttgc atcacagtta aatgttattg ccgagtgtcc tgatattgct caatcccata
     6841 caaatgcgat aacacttgat aaattctgtg ctgatgttga tgtcgtgcta ggaatcaatg
     6901 ttgtcagtaa ggatggcggt gcctttgtag gtaccaaaat tagagcgatg gctgaagcat
     6961 cgggttttaa acttgaatca gaaggtatgt ttagattccg ggatgaaaat aatgcaacat
     7021 tattttcact ttgtaattat gaaacaacac ctttctcccc tgccaacatg cggacgctca
     7081 ccactcacgg catcaccttt ttatttgatg taccccgcgt tgcaaatggc gaaaaagttt
     7141 ttgaacaaat ggcccatctt gccaaacttt ttgcctcaac cttaaatggc attatggttg
     7201 acgacaatcg tgtgccactg agtgataatg gtctaaagcg aagtaaacag caattgaaaa
     7261 atattcaagc tgcaatgtca gcccataaca ttctagccgg cagcgacgtt gcacttaagc
     7321 tatttgttta acattaataa ctgaactaag aaggtcatcc aatgagcgaa attaaaaaat
     7381 tttctccgat tgaagtaaca ctcgaagcca atcggcaata ttattggtgt tcatgtggtt
     7441 taagcaaatc gcaacctttt tgtgatggtt cacacaaagg aactactttt acacccctcg
     7501 ccttcagcgt tgaacaaact aaatctgtct ggctttgtac ctgtaaaaaa actgctaata
     7561 aaccctattg tgatggtacg cataataaaa tttaaccaga atcaactttt aggtatttag
     7621 ttaaatttat gcatgtaagc tggtctgaat tatactggca gcgattaaca ccacttcatg
     7681 cagttttatg gccaattagt atactgcttg agctatcatt taagcttaaa cgctcaacct
     7741 atcgctatgg tctgctttcc ccaatacgat atcccgttcc gattatcatt gttgatagca
     7801 taacggttaa tagcattgat aagtcggcgt tcactgtctg gttagtaaat tttttaacat
     7861 ctaatgggtt gcgacctgct gttattagct gtagctaccc tgatagctat caggaatcgc
     7921 caattgaagt gacaacttcg agcgataaaa ctcaagttgg agatactgct tcattcctgg
     7981 cttttcattg tagtaagcat cattgccctg tctggatcgg acaggatatt agtacggttg
     8041 gattcgattt actaaaggct caccctgatc ggaatgtatt gatatgcaat aacgggttgc
     8101 atgactatcg tttgtttcgc gatttagaaa tattagtttt agatagcgat aaacattatt
     8161 ttggaaatgg ctttactttg ccagctggcc cacttcgaga taatcttacg cgtttaaatt
     8221 tggccgatgc aattgtttta aacgaaaaaa ataggcagtc ttttgaacaa gtcgataact
     8281 caaaaattta ctatgtcaaa accagtagta gcgaatttat taattttttt catactagcg
     8341 atcattttaa aaaattaact acatcgggcc agaaactaca cgctattgcc agcattaaac
     8401 atgctaaacg tttttttgac cagcttgctt atttgaatat tactgcaagc aaacaatcga
     8461 tactgaatga atcacaattt accaaacgtt atattgattc acttaacgca gatattatct
     8521 tgatcaaaga agaagatgcg gttaaatgtt tgagttttat tgatgatcgt ttctggattt
     8581 tgaaagaatc gattgttgca aatacagcgc tacaagtaac gttattgaac aaactgagag
     8641 aaaaatttat ggatactaaa ttattggata ttttggtttg ccccttatgt aagggttctt
     8701 tgatctataa aaaagtggaa aaagagctga tttgtaaacg tgaccgaatg gcgtttccta
     8761 ttcgtgatgg cattccaatt atgctagagg atgaagctcg cttacttcca cctgaagaag
     8821 aagtttaatc ttcttgaaat ttcctgaaaa caactacgaa tctaagtcca tatcatcggt
     8881 gaaagtaagc aacttctttg tactgtcatt ggatcagaca cttcatggct tgtttaatgt
     8941 aataacaggt catttatcac cttatttact tcaatttctt ctacttcaaa atcatcgtca
     9001 gtaaaatcag tatcatcaat aactttcatc actcgagtgt ccggaatttg ttcatcgatt
     9061 gattccggaa aatcagatag ttgagtaata atttgtggtc tcattttaaa tattctccat
     9121 atagaatttt aataatgaag tgcaatgtgc ttaccattaa tctatacgga agaattgaaa
     9181 ttaaccgtga gaaaaaacac aaagattttt ccaaaaaata atttttttta ttgtcattaa
     9241 caccgtattc tcacccagta gccatggcgt ttgtagattc tttttctaag ttgacttaag
     9301 ccacacggaa tttggatata atttagtaat tatttagcct tcatgaccaa ggctcggaat
     9361 taatgaccaa taagcatctc tctgcaacaa tagcctagaa tatttcttaa accatactat
     9421 cgatatactg caaaatgaat gatcagcaac ttaaaatcct tatcaagctt aaccaaattc
     9481 ctcagtcccc agtatctacg attgccgata attctgaaat aactgaaagc catgatgaag
     9541 aaaaactgcc ctttgattgg cttaagattt cagcagcctt attgctatta ttcctgctat
     9601 taggagtgag ttattggtta ctaaccggta gcgagaagga atcagctgaa tcatcgattt
     9661 tctcaaatga agataggagt caatccactg atagcaatca acaagcacct tctgatctag
     9721 aatcaatcgt tgacgcgact tcacccgaaa cagccagaac gatagtggtc cccagtgatt
     9781 tagggaaaca aactgagtcc gtaagtaatg ctgtcactag caatgaaaat catgctcagc
     9841 aagcgatgat tattccttca tccaaacccg agtcaaaagg gttgaaattg aataattcgc
     9901 aatccaaaga aaaaccaacg cagacgcagt caaattacgt tataaaagca caattgacta
     9961 catcaatccg aaaacaagaa tctattaatt caattgatca tattgaactc caacgaggaa
    10021 tgaatcagac tatttattac tccttgcata ttcgtaatct aaagggggaa aaaatattcg
    10081 tgaattggta ttaccaagat aaacaggttg ctaaaattcc gttttcaatc aataccaatg
    10141 attggcaagc ccactcaagt aaaattttaa ctaaaacaag cttgggtcga tggcgtgtta
    10201 cagcaatgga tcagttaggt aatattttat ccgagcaatt tttcttggtc agcaatcgtt
    10261 cttgattcat ctcatggcgt gagttttaac ctaaattaca attagcaaac acatgaatta
    10321 ttgtagccat tgcagtgctc ccgttgagtt aagaattcca gatggcgatt ccctaccccg
    10381 cttcgtatgc ggaacctgca acaccattca ttaccaaaat cctaaaatca ttgtgggatg
    10441 tatcccagaa tggaacaatc aaatcctatt atgtcgacgc gcaatagaac caaagattgg
    10501 attatggaca attccagctg gatttatgga aaatcaagaa acattagcag aggctgcgat
    10561 tcgtgagacc aatgaagaag ccaatgcaaa tattgaaatt ggtgacctct atgcagttta
    10621 tagcttgccc catatcagtc aggtttatgt tttgtatcgg gcaaaattaa tcgataccaa
    10681 tttttttcca ggtatcgaaa gtcaagaaac caaattgttc catgagcatg agattccttg
    10741 gggtgatatc gccttcaaag tcattcatga ttctttaaaa cgctttatag aagaacgccg
    10801 tcaaaatagc attcagttct atacgggcgt catcactcgc gctccatcgc aataaaattt
    10861 atttcttaac attgggtaaa ttattaccca atccaccgat acgtttcgca gtatttcata
    10921 ggaccagtag gctcaatagc agtcacctgc tatggttact gtgaaatgtc cctgatttta
    10981 gcgttttcaa ttcaattcaa gcgcatacct caactgataa attagcaaat tagtcctttt
    11041 gctacttatt ggcattaatt atcactctta agttcttatc atgcgctgta tttatttaac
    11101 tttagttttg actttttttt cgtttcaagt gctagcttcc aagcaatttg aagatatgag
    11161 tctggaacaa ttaatgaatg tcagtattac aggcgcctcc aagtatgagc agaaacaaag
    11221 cgaggtggct gcagcggtta cggttattac gcgtaatgat attcgagctt atggttggag
    11281 aacattaagt gagatattaa caagccttcc aggtgttttt gctacttatg attttagcta
    11341 caactatatc ggtgtaaggg gttttaatgt tctcggcgat tttaatacgc gtattttagt
    11401 ttcaatcaat ggtaaccgaa tcaatgatgc gacttatgat caaggtccta tggggcggga
    11461 ttttccgcta gatgtcgatt taatcgaacg catcgagttc atcccagggc cggggagcgc
    11521 ggtttatggg cagaatgcaa tgttaggtgt cgttaacatt attactcgca aaggctctaa
    11581 tgtgaatggc gttgaacttt ctgggtctta tcaaactgca gaagtaatgc cccaagaacg
    11641 tgccacctta gggaaaaaat tcgataacgg tgtcgatgct ttagtttcgg tttcaggcgt
    11701 tcaagcacgc ggtgaaaatc ttttttttga ttttcgtgat gctgctattt caggcgttgc
    11761 tcaccatatg gatggcgaaa atattaagca gatttttgca agagcagaaa aagggcccgt
    11821 ttcctttgat tttatctacg gtaatcgcaa aaaagacgac cccacggcaa tttatttttc
    11881 agacccgctc atttctgggc aataccttaa agatcggcgt ctgaattctc aattgcaata
    11941 taacgataat tttgtcaatg acacgcttaa tgtacttggg cgacttttca tcggtaaata
    12001 cgattacgat agcccattag tttatgaggg gaaaaaaaca ttatcaacgg gtccaagttt
    12061 ctggcatggt gctgaattaa ggttagtttc aagcgcaatc acaaaccata aattgatggc
    12121 cggtgttgaa taccagaata atacacatat caagcagaca tttgagtatt tggagagcct
    12181 cgcggataat attaccgtta gaagctcagt agttagaaca ggggtatatc ttcaagatga
    12241 atggcgcatt accgataccc tatcatcaac attgggggtg agatacgatt acaacaactg
    12301 gatcggcaat cgcctaagtc ctcgtggtgc tttaatctgg caagctacac ctaaagctgc
    12361 atttaaagcc atttacggtc gtgcacaccg atcccccaat tcctatgagc gtgattatgg
    12421 agatggcgtt tctcaaatct caaacccagg gttaagagga gaaaccattg atgcagcgga
    12481 gttagtcgca gattactttc ctgaacccaa tctaaatttg cgggcttcgc tatatgcttg
    12541 ggatgtttac aacattatcg cattgggttt taatccgttg ttggaagctt cccagtatca
    12601 gcaaacccac gacaaagtac gcgcaagagg ggtagagctc tattttgata aaacctggaa
    12661 ttggggagct cgcctacgaa gtagcttcgg tattcaagat gcttcacaaa aaggatcgca
    12721 tattcctaat tctccccagc tattaggaaa gattaacgtt ttggttccaa ttccacttat
    12781 gtctggccta aaaggcggct atgagcttca atatttcggt gaaagaaaaa cgttaaatgg
    12841 tcccaacacc gatagttatt ttctctctaa cttaaactta atgacagata ttcgttgggt
    12901 gaaaggatta gaggcatcgc tcgctgttta taatttattt aatgagcact atttacatcc
    12961 ggtttcagac tcaagctggc acaatagttt attgcaacct ggtagaacgg ttagatttag
    13021 gctcgagtac cgtttttaat taacaatgat tagtcggatt attaacatcg attgcaaaac
    13081 aaatagcaat taatgcaatc gatcagcata aataagccaa aaaaaccttt taaatgtaaa
    13141 cagaactcta gctatcatgg gtttatacta atagtcaatt taataataac attacccatt
    13201 tggcttaccg ctgtgcttta ttcacccaat tcttttggac aacccgaaga accgactgaa
    13261 taccggttga aagctgcatt tctttataat tttgcaagct ttcccgagcc acctactaag
    13321 attgataaac acagtacttt caatatgtgt ttcctcgatg acaatccatt tgatccctac
    13381 cgcgattaca tcactcaaag aaaagtaaaa gacttgcaag ttaccatttt attgaagtct
    13441 gataccgata aattaagtga atgccatctt ttgtacattt cccgctctgt tgctagcaat
    13501 aagcttgaaa gcattcttga ccgcataagc agtggcccta ttttaacggt tgctgatagt
    13561 acgggaatct gccaaaaaga agttgtaact aatatgggga ttaatgcggg caaaactacg
    13621 ttcaaaacaa atttgacgcc tgctcacaca gcaaaattag aactcagctc acaattactt
    13681 cgctttgcta aagaggctta tcaataatga atcccctatt acctgctaca accacaataa
    13741 aaaataaact acaaaaaatt agtttgattt cacaaagtac agcaatgttt ttacttgctg
    13801 gattggttat attgagtagc tttatcatta atctttactc acttttggag acgagtcagt
    13861 ccaccgcgaa aatcctggca gaaaatgcaa ctgcaacttt aatgtttcaa gataaaaatt
    13921 ctgcacaaac attactctat tcactaaata acctaagccg agtcaatgcg gcggctattt
    13981 tcagtgagaa tcatcaccaa tttgcagaat acccaaataa ccatcatgct tttccccatt
    14041 ttgattctct aaatgaagaa aaagtcttaa tcgatgcaca atatatcaaa ttacttcaac
    14101 caatccgctt caatgatcaa tcattagggg cgctctattt agaagtcacc atgtt
//
LOCUS       DAOQLJ010000050        14074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1597_length_14074_cov_6.622512, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000050 DAOQLJ010000000
VERSION     DAOQLJ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 14074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14074
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1597_length_14074_cov_6.622512"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PK522_05170"
     CDS             <1..649
                     /locus_tag="PK522_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC41156.1"
                     /translation="IRWNHPQRGLIQPDKFIKLAEGTELMGKLTLWVLNTALRHCASY
                     RNAGIDAGVSINFSADNLLDPELPDLVLQGIEIWKIPPDKVMIELTESAIMEDRPGSL
                     ETLHELKKLGLKLAMDDFGTGFSSMERLLNLPLDEIKIDMMFVKDMLTSPIHERIVDS
                     MINLGHQLNLIVVAEGVEDAQTYERLKQLGCDVIQGYFIGRPVPLTELIQQVTNK"
     gene            complement(683..2581)
                     /locus_tag="PK522_05175"
     CDS             complement(683..2581)
                     /locus_tag="PK522_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidotransferase 1, exosortase A
                     system-associated"
                     /protein_id="HNC41157.1"
                     /translation="MCGIVGIFDTQGKREIDRRILNELNQTQTHRGPDEGELYLEPGI
                     GFGHRRLSIMDVSSGQQPLFNEDGSVVVIFNGEIYNYQELVKELVACGHQFRTHCDTE
                     VIVHGWEEWGEQCVDRFRGMFAFGVWDRNQDTLFVARDRLGIKPLYYALLKNGLFIFS
                     SELKAIHTHPEFVKEFDHFAIEEYFAYGYIPEPRTIYKDAQKLSPGYSIKLSPHSKQI
                     KPRQYWDIPFSPHSDMSEQAVEQELITRFREAVDIHLMSEVPLGAFLSGGVDSSAVVA
                     MMSGLMKDSVNTCSISFANPVFDESKYAQEVADRYHTKHYVDQVEQDDFDLIDKLAFL
                     YDEPFADSSAIPTYRVCELARKRVTVALSGDGGDENFAGYRRYRWHMYEEQLRSRLPI
                     GLRKPVFGLLGRLYPKADWAPKFLRAKATFEALARDSVEGYFHSVSIMDDNMRDQLFS
                     RDLKDTLNGYKAVEILRHYANKSPTRDPLSTIQYLDMKTYLVGDILTKVDRASMAHSL
                     EVRVPLLDHKLVEWISGLPASMKLSNNQGKYIFKKALEPYLPNDVLYRNKMGFSVPLA
                     EWFRKSLRNRVRESLLGKTLAETKLFNMDFIEKMIDQHQSGRRDFSAPLWTLLMFESF
                     LRSVHNKA"
     gene            complement(2643..3791)
                     /locus_tag="PK522_05180"
     CDS             complement(2643..3791)
                     /locus_tag="PK522_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03088 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNC41158.1"
                     /translation="MNQSISPPLIAHVIYHLGVGGLENGLVNLINHLPQDRYRHVIIC
                     LKGYTDFSKRIKRSDVEVFDLNKPDGLNFSWYVSLYRLLRQLRPDIVHTRNFSTLEGQ
                     IVAALARINARVHGEHGRDILDLEGKNRKYNLLRQSIRPLIHRFITVSKDLERWLINT
                     IKVEPAHIKQIYNGVDNIRFSPKSKLNSITIPNNFIADNTFVIGSVGRMAKVKDYLSL
                     VRAFLLLVERIPQAKDHLRLLIVGDGESRKECIAMLRQADLENLAWLPGERSDVSELM
                     QLFDVFVLPSLAEGISNTILEAMSCGLPVIATSVGGNVELVIDSQTGKLVPSGNPEAL
                     ATAILEYYNHREMLIKHGQAARQLIETNFSMDTMVAGYLDVYDEIISR"
     gene            complement(3814..5391)
                     /gene="xrtA"
                     /locus_tag="PK522_05185"
     CDS             complement(3814..5391)
                     /gene="xrtA"
                     /locus_tag="PK522_05185"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HNC41159.1"
                     /translation="MNTMILPEASRTASLKELKESIYIVLMVIVAIFFIFYEATESIV
                     STWIRSETYAHGFLIFPFSLYMIWKKRTTLYGLAHYPEFKWLIVLGILGFGWLLAFLS
                     SVLVVKQFVLISMIPIVVCAIMGFRFAWAIAFPLAYLFFAVPVGESLIPPLIDFTADF
                     TVSALQMTGIPVYREGNFFSIPSGNWSVVEACSGLRYLIASVTLGTLYAYLTYISWVK
                     RLIFIIFSIIVPILANGLRAYLIVMTGHLSDMKLAVGVDHLIYGWVFFGFVMLLLFWM
                     GSLWREDDLEPQCEIQGSITVSSLEASASLKLKLLVACLVIASLIIWPIYGRHLDEQK
                     QEKETVDIRLVDRLGRWVPISQPISNWIPIYVGQPSKHYYHFKYNNQLVSVYITYYRN
                     QNEDSELINYANLLVEEMDPVWRQIEKSKYTLSVSSKKLEISQHQLTSHQSKLLIWRW
                     YWLINEQTANPYVAKANLAFNRILGYGDDGAEIILAAPYVNRPEEAAIVLQNFTNDMM
                     PAIMESLQLARANDSRH"
     gene            complement(5437..6633)
                     /locus_tag="PK522_05190"
     CDS             complement(5437..6633)
                     /locus_tag="PK522_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNC41160.1"
                     /translation="MQKLLYLTHRIPYPPNKGDKIRSYHVLQHLRKHFKVYLGTFIDD
                     EQDWQYVNHLNSLCEETCFVRLDPLLARMRSLIYLFSSMPLSLPYYRSSKLSTWVSNQ
                     LSSGTIKHTVVFSSAMAQYISETGIACSVIDFVDVDSDKWSQYASTKVWPQKWVYQRE
                     ADLLLEYEKYVTKKCSYATFVSEKEAGLFKQLSPEVENKITYFNNGVDTEYFSPSQNF
                     ANPYKPGTRVLVFTGAMDYWPNVDAVRWFAAELFPAIRTKIPDLEFYIVGARPTKTVL
                     ELSAIPGVVVTGSVDDVRPYLAFSEIAIAPLRIARGIQNKVLEAMAMEKLVVASPQAL
                     EGITAIPGIELFVADGAEAYIDHVISLLENPRMEAGKAARRRVIEDYNWDKNLSRIDR
                     LLQVSS"
     gene            complement(6643..7725)
                     /locus_tag="PK522_05195"
     CDS             complement(6643..7725)
                     /locus_tag="PK522_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HNC41161.1"
                     /translation="MSSIDTTLLESINKIAVTDSIKISLLNPDVASLWDDFVVSCKEA
                     TFFHRAGWKSVIENAFGHKTWFLYAERDQQIIGVLPLVEVNSHLFGHSLSSLPFCVYG
                     GIAAADDQARKLLDQAAQKLAIQLAVDYLEYRNITPYHSDWVTKGLYVTFRKEIDVDA
                     EKNMLAIPRKQRAMVRKGIKNNLQGSIDTDIDRFFYAYSTSVHRLGTPVFSKKYFRLL
                     KSTFGEDCELLIISSDHKVVSAVMSFYFRDEVLPYYGGGIDEARDLAANDFMYWELMR
                     RSCERGISIFDFGRSKLDTGSYSFKKNWGFEPQPLYYEYQLHKVKTMPDHNPLNPKYR
                     LFIQAWQRMPLALANFIGPHIVKNLG"
     gene            complement(7722..8561)
                     /locus_tag="PK522_05200"
     CDS             complement(7722..8561)
                     /locus_tag="PK522_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HNC41162.1"
                     /translation="MIRNALTIDVEDYFQVSAFDPYIPRNDWDSIPCRVERNIDRILS
                     LLEKKGINATFFTLGWIAKRYPSMVRRIVENGHELASHGCDHDRVTELGSDDFYKDIA
                     DSKKILEDISGFPVIGYRAPSFSINKQNYWALNLLEEAGYLYSSSIYPVRHDHYGIPD
                     APRFAFYPNPTGKLLEVPITTVNLLGRNIPVGGGGYFRLWPYAVSRWFIQRVNEKDQR
                     SAVFYFHPWEIDPDQPRQKGINLKVRFRHYLNLDLMEKRLTNLLNDFGWGRMDQIFLE
                     KNR"
     gene            complement(8661..9509)
                     /locus_tag="PK522_05205"
     CDS             complement(8661..9509)
                     /locus_tag="PK522_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated ATPase"
                     /protein_id="HNC41163.1"
                     /translation="MYLSFYGLKTKPFQLKPDASFFFSSKGHKRAIAYLEYGISQEDG
                     FIIITGEVGAGKTTLIRHLFQKLDQQRFIVAQLANTNLDSDDMLRMVAASFGLPVTNT
                     SKATTLLEIEQLLRQYDKEGKKILLLVDEAQNLTSRALEELRMLSNFQTDDKLLLQTF
                     LLGQPEFRKTLLGNNMQQLRQRVIATYHLGPMDNTETRAYIEHRLAIAGWQGSPLLQD
                     DSFDVIYNFTGGIPRKINTLCDRLLIMGCLEELRTITSQEVNEVVADIQQEFDLPPFQ
                     NIETSD"
     gene            complement(9535..11094)
                     /locus_tag="PK522_05210"
     CDS             complement(9535..11094)
                     /locus_tag="PK522_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03016 family PEP-CTERM system-associated
                     outer membrane protein"
                     /protein_id="HNC41164.1"
                     /translation="MKLLSKHFCNYHCKRLIYISILLFNIIALPIYAGEWRVVPSLNI
                     NERYTDNLRLGGIATGGKGNDEFITQINPSILINGNARRYRVDVNYLMNNLIYANNSE
                     FNRIRHQLNANGTIELIQDRFFIDGRAVILQQNASLLGSQGLDNANPLNRRNMSMLNI
                     SPYYRHRFGDFATAELRYTHGEVNTSGGGLRDSSTDNGTVRLNSSTAFRDLQWGITYN
                     HTEIHRSQLRTIALERSTVLLRYMLTPQFSLVGTGGYERNSFISVNGGTSSPTWTVGF
                     SWIPNERTEFDFAGGQRIFGDTYLANFSHRTRLTTWSASYNEDITTFGQQNLLQPGAA
                     GAIPSGGILPQLLLNTMTNAGFGGGLANQGALLGISGANNFFTNRLFLQRNLQAFMTI
                     NGSRNTIVFRGFNISRQAYTGDEEDDSIGGRVNSVLNRNTVQTGGGVSWIYKLSPLMN
                     FTTNYTFTRINFTTTNLTDINQIMMVSLNKRFSPNLFGEISYFHNRRDSERINGDFSS
                     NNYMASLSMNF"
     gene            complement(11091..12131)
                     /locus_tag="PK522_05215"
     CDS             complement(11091..12131)
                     /locus_tag="PK522_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated tyrosine autokinase"
                     /protein_id="HNC41165.1"
                     /translation="MSIIERAADKLKSNADAEQKIPAIKNIHKDIIQDSKRKIQKEAS
                     DIERITEKFKNTIADSPKIQPIEPQGVDNAVKINREYIDKPISITEGRTVGKKVSVDT
                     HLPQPHIDIDLDRLHKLGIVTPQHGKTQIAEQFRIIKRPLLTNAFSKNSGIKNGNLIM
                     ITSSLAGEGKSFCSTNLAMSIAMEMDNRVLLVDADVARPSIPKMLDFSPDRKGLLDII
                     LDQRLNVSDILTKTSVDKLTLIAAGTRHPHATELLASQSMADILMELAQRYSDRIVIF
                     DSPPLLLTSEARVLASQMGQIVLVVEAERTTQQTVKEALRQIESCDVINLIYNKARTS
                     AAAGYYGYYYSS"
     gene            complement(12173..13735)
                     /locus_tag="PK522_05220"
     CDS             complement(12173..13735)
                     /locus_tag="PK522_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HNC41166.1"
                     /translation="MDELIAQLLVYIKGTWKYRWVSIAIAWLVSIIGWTVVHHLPDDY
                     EASARIHVDTQNILRPLMAGMTVSPNPEQQVAIMSRTLISRPNVERVIRMVDLDVKVT
                     DDVAYDKLVTSLMKDIRLGAAGRENLFTISYSHKDPKLAKDIVQSLLTIFVEGGLESK
                     KQDSTSALRFLDQQIASYEEKLIASETALTGFRQRNIGLMPGQSGDYYAQLTNALEEL
                     EKAKVALKEAEQARDAVRRQISGDETVLLLETHENVLEKIINPEIDARIQALNLNLDN
                     LRLNFTDLHPDIVATKRLITQLEERKKEEATLIDPTAVDQGRNFSPMLQQLNIALTEA
                     EAVVASMQARVSEYTNRFERLKSLSSMIPTVEAELTQLSRDYNVNKANYEKLLERRAS
                     AEISGELTSTTGLMSFRIIDPPVVPETPSGPDRKKMYSFVFIGALLAGIGVAFIISQI
                     RPTFHSQTDLRQVTGLAILGTIPMVWTDQEKSKRRNRLYAFGFSLLVLMALYTTLMIY
                     TKLPVATLINLF"
     gene            complement(13785..>14074)
                     /locus_tag="PK522_05225"
     CDS             complement(13785..>14074)
                     /locus_tag="PK522_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNC41167.1"
                     /translation="TGFVGPFTEQIRVIGEATRPQALPYSENMTLMDVMIAVGGITDF
                     AAGNRARIIRTIDGKQQQFRVRLNDLIRDGDITANTPVYQGDILVIPESFF"
BASE COUNT         4321 a         3064 c         2450 g         4239 t
ORIGIN      
        1 catccgctgg aatcatcctc agagagggtt aatacaacct gacaaattta taaaacttgc
       61 cgagggaaca gaactgatgg gtaagctcac actctgggtg cttaataccg cgcttaggca
      121 ttgcgcttcc tacagaaatg cgggcattga tgcgggtgtt tccattaatt tctctgctga
      181 taacctgctt gatcccgaat tacctgatct agtcttgcaa gggattgaaa tatggaaaat
      241 tccacccgac aaagtcatga ttgaattaac tgaatcagcc atcatggaag atcggccggg
      301 ctcactggaa acgttgcatg aacttaaaaa actaggatta aaattggcca tggatgattt
      361 tggtactggt ttttcttcaa tggaacgctt attgaattta ccccttgatg aaatcaagat
      421 cgacatgatg ttcgtaaaag atatgctaac ttcgcctatt cacgagcgga ttgtagattc
      481 gatgattaac ttgggacacc aactcaattt aatcgttgtg gcagaaggtg tagaagatgc
      541 tcaaacttat gaacgcctaa agcagctggg ttgtgatgtg attcaaggat attttattgg
      601 acgcccagtt cccctgactg agcttattca acaagtaacg aacaaataaa atctagccac
      661 cattaccaag acaaaatagc atttatgcct tgttgtgcac gctccgcaaa aaggattcaa
      721 acattaataa tgtccataaa ggtgcgctaa aatcccttct tcccgattga tgctgatcaa
      781 tcattttctc aataaaatcc atattaaaca gtttggtttc tgctaatgtt ttacccagta
      841 atgattccct cactcggtta cgtaagcttt ttctaaacca ctccgctaag ggaacggaaa
      901 aacccatttt attgcgataa agtacatcat tgggaagata aggctcaagt gctttcttaa
      961 aaatatattt cccttgatta ttagataatt tcatcgaagc aggtaatccc gatatccatt
     1021 caaccaattt atggtctaac aagggtactc ttacctctaa tgagtgcgcc atactggctc
     1081 gatcgacttt cgttaagata tcccctacaa gataagtctt catatccaaa tactgaatag
     1141 tcgataatgg atcacgagtg gggcttttat tcgcataatg acgtaaaatt tcaacagctt
     1201 tatagccatt caaagtatct tttaaatcgc gactaaagag ctgatcacgc atattatcgt
     1261 ccataatcga aacactatgg aaataaccct cgaccgaatc gcgtgcaagc gcttcaaagg
     1321 ttgccttggc acgaagaaac ttgggtgccc aatctgcttt tggatagagc ctccctaaca
     1381 aaccaaatac tggttttcgt aacccaattg gcagtctcga tctcaattgc tcttcgtaca
     1441 tatgccatcg atagcgacga taacctgcaa aattttcatc accaccatcc cctgataaag
     1501 caacggttac tcgtttcctt gcaagctcgc acacacgata ggttggaatc gcagagctat
     1561 cagcaaaagg ctcatcatat aagaaagcga gtttatcaat tagatcaaaa tcgtcctgtt
     1621 caacttgatc tacataatgt tttgtatggt aacgatctgc tacttcttgc gcatatttag
     1681 actcatcaaa gacaggatta gcaaatgaaa tcgagcacgt attaacagaa tccttcatca
     1741 aaccgctcat cattgcaaca actgcactgg aatcaacacc tccagataaa aatgcaccca
     1801 gcggaacttc actcattaaa tgaatatcca ctgcctcgcg gaaacgagta atcaattctt
     1861 gttcgactgc ctgttcgctc atatcagaat gaggtgaaaa cggtatatcc cagtattgac
     1921 gcggttttat ctgtttgcta tggggtgata attttatcga atagccagga gagagctttt
     1981 gggcatcttt atatatcgtt ctgggctctg gaatatagcc ataggcaaaa tattcttcaa
     2041 tcgcaaaatg atcgaattct ttaacaaatt ctgggtgagt atgaatcgcc ttgagttccg
     2101 atgagaaaat gaatagacca tttttcaata atgcgtaata cagcggttta attcccagtc
     2161 gatctcgagc aacaaacaaa gtatcttgat tacggtccca gacgccgaaa gcaaacatac
     2221 cacggaatct atctacgcac tgttcacccc attcctccca tccatgcacg atcacttcgg
     2281 tatcgcaatg ggtacgaaac tggtgaccac aggcgactag ctcttttact aactcctgat
     2341 agttatagat ctcaccatta aaaatcacaa caacacttcc gtcttcatta aacaacggct
     2401 gttggccact tgatacatcc ataattgata atcgccgatg gccaaatccg ataccgggct
     2461 ccaaatagag ctctccttca tctggcccac gatgggtttg cgtttgattt agctcgttta
     2521 gtatccgacg atcgatttcg cgtttacctt gcgtgtcaaa aatacctaca attccgcaca
     2581 tacgagcaac ctctcccgat taatttataa tttttccaat tttgttaatt gatagatgct
     2641 aattatcgac tgataatttc atcgtagaca tcaagatagc ctgcaaccat agtatccata
     2701 ctgaaattag tttcgattaa ttgacgagcc gcctgaccat gctttatgag catttcccga
     2761 tggttataat attccaatat cgctgtagca agcgcttctg ggttacccga tggtactaat
     2821 tttccagttt gtgaatcgat tacaagctcc acattgccac caacacttgt agctataacg
     2881 ggcaatccac atgacattgc ttctaaaatt gtatttgaaa ttccctcggc aagggatggc
     2941 aaaacaaaaa cgtcaaataa ttgcattagc tctgatacat cagaacgttc tcctggtaac
     3001 caggccagat tttctaaatc tgcctgacgc aacatcgcta tacattcttt ccgtgattcg
     3061 ccatctccga cgattaaaag tcttagatga tcttttgctt gaggaattcg ttctactagt
     3121 agtaagaaag ctcgaaccaa actaagataa tctttgactt tagccattct ccctacactt
     3181 ccaatgacaa atgtgttatc agcaatgaaa ttattgggta tcgttattga atttaacttg
     3241 gatttagggc tgaatcgtat gttatcaaca ccattataaa tttgcttgat atgagctggc
     3301 tcgactttta tcgtattgat aagccatctt tccaaatctt tacttaccgt aataaagcgg
     3361 tggattaaag gacgaataga ttgtcgtagt aaattatatt tacgattttt tccttcaaga
     3421 tctaagatat ctctgccatg ctctccatga acacgtgcat tgattctcgc caaggccgca
     3481 acgatctgcc cttccaatgt agaaaaatta cgtgtgtgta cgatatcggg tctcaactgc
     3541 ctcagtaaac gatataagct aacataccag gaaaaattta acccatctgg cttgttaaga
     3601 tcaaaaactt ccacatcgct tcttttaatt cgtttagaaa aatcagtata gcccttgagg
     3661 caaatgatga catggcggta acgatcttga ggaaggtgat taatgagatt aaccaaacca
     3721 ttttctagcc cacctacacc taaatgataa ataacatgcg caataagcgg gggagatata
     3781 gactgattca tcatattgat tagaaagatg ttgttagtgt ctactatcgt tagctcgtgc
     3841 taattgaagt gattccatga tcgctggcat catatcattc gtaaaatttt gtaatactat
     3901 cgctgcctct tcaggtcgat taacataagg ggcagctaaa ataatttcag ccccgtcatc
     3961 gccataacct aatattctat taaaagcgag attagcctta gcaacataag ggtttgctgt
     4021 ttgctcatta atcaaccagt accatctcca aattaacagt ttagattgat gggaagtcag
     4081 ttgatgttgg cttatttcta attttttaga actaactgat agcgtatatt ttgatttttc
     4141 aatttgtcgc caaactggat ccatttcttc caccagtaga ttagcgtaat taataagttc
     4201 cgaatcttca ttctgattac gataatatgt gatatacaca ctaaccagct gattattata
     4261 tttaaaatgg taataatgct tacttggttg accaacatag attggaatcc aatttgatat
     4321 tggctgcgaa attggaaccc atcttcctag tcgatctacg agccgaatat caacagtttc
     4381 tttttcttgt ttctgttcgt ctaagtgcct gccataaata ggccaaatta ttagcgaagc
     4441 gatgactaaa caagctacaa gtaactttaa ctttagtgaa gcgctggcct ctaatgaact
     4501 cacagttatc gatccctgaa tttcgcattg aggctccaga tcgtcttccc gccaaagaga
     4561 acccatccaa aacaacaata acataacgaa accaaagaaa acccaaccat atataagatg
     4621 atctacccct accgccaact tcatatcact caaatgccct gtcataacga tcaagtaagc
     4681 tcggagtcca ttggctagaa tgggtacaat aatagaaaag ataataaaaa ttaatcgttt
     4741 tacccaactg atataagtca aataagcgta gagcgttccc aaagtaacgg acgcaatgag
     4801 atagcgtaac ccactacaag cctccactac agaccagttc ccacttggga tactaaaaaa
     4861 attaccttcc cgatagacag ggattccagt catttgcaac gcactcactg taaagtcagc
     4921 agtaaaatcg attagtggtg gtattaagga ttccccaaca ggaacggcaa agaaaagata
     4981 agcaagggga aatgctatag cccatgcaaa tctgaaaccc atgattgcac aaactacaat
     5041 gggtatcatg gagatcaaaa caaattgctt aacaacaagt acgctagaaa gaaaagcaag
     5101 aagccaccca aagcctaaaa tacccaaaac gattagccat ttaaactcag gataatgagc
     5161 tagaccataa agtgttgttc gcttcttcca aatcatatac agactaaatg gaaaaattaa
     5221 gaagccgtgt gcataagttt cagatcgaat ccaggttgat acaattgact cagtcgcttc
     5281 ataaaagata aagaatatgg cgacgataac catcaacacg atataaatcg attcttttaa
     5341 ttcttttaaa gatgccgttc tcgacgcctc aggtaaaatc attgtattca tttcgtaatc
     5401 aattgttagc cactatggca agaatcgaat tttgtgttaa ctcgatacct gaagtaagcg
     5461 atctattcta gatagattct tatcccagtt ataatcctct attactctgc gacgcgctgc
     5521 tttccctgct tccattcggg ggttctctaa taagctgatc acgtgatcga tatatgcttc
     5581 agcaccatct gctacaaata gttcaattcc gggaatggca gtaatacctt ccagcgcttg
     5641 aggtgaagca acaactaatt tttccatcgc catcgcctca agcactttgt tttgaatccc
     5701 tcgcgctatc cgaagtggcg caattgcaat ttccgagaat gctaaataag gtcttacatc
     5761 atcaacactc cccgtcacca ctactcctgg aatagcggat aattccagta ccgtttttgt
     5821 aggtctagca ccgacgatgt aaaactccaa atcaggtatc tttgtacgta tcgctggaaa
     5881 tagttcagct gcaaaccatc ttaccgcatc gacattaggc caatagtcca ttgctccagt
     5941 aaacaccaag actctagtgc ctggtttata gggattagca aaattttgac tcggtgagaa
     6001 atactcggta tcaacgccgt tattgaagta agtaatttta ttctctactt ccggagataa
     6061 ctgcttaaat aaaccggcct ctttctctga aacaaaggta gcgtaactac atttcttagt
     6121 gacatatttt tcatattcaa ggagcaagtc agcctcacgc tgataaaccc atttctgagg
     6181 ccacacttta gtcgacgcat attgactcca tttatcagaa tcaacatcta caaaatcaat
     6241 tacgctacac gcaatgcctg tttcgctaat gtactgagcc atcgcagaag aaaaaacgac
     6301 tgtatgcttg atcgttccgc ttgacaattg attgctaacc caggtactca gcttggaact
     6361 tcgataataa ggcagcgata atggcatact cgaaaacaag taaatcaaac tacgcattct
     6421 cgccaatagc ggatcaagcc tgacaaagca agtttcttca cataatgaat tcagatgatt
     6481 cacatactgc caatcctgtt catcatcaat aaatgtacct agataaactt taaaatgctt
     6541 tcttaagtgt tgtaatacat gatatgaacg aatcttatcc cctttattgg gggggtatgg
     6601 tatacggtgt gtaagataaa gcaatttttg cattgctttt ctctagccta gattttttac
     6661 tatatgtggc ccaataaaat tggcaagtgc caaaggcatt ctttgccagg cttgaataaa
     6721 gaggcgatac ttaggattaa gtgggttatg gtctggcatt gttttgactt tatgtagctg
     6781 atattcgtag taaagtggtt gaggttcaaa accccagttt ttcttgaaac tataggatcc
     6841 tgtatcaagc ttgcttcttc caaaatcaaa tatgctgatt cctctttcac aagagcgacg
     6901 cattaattcc cagtacataa aatcatttgc tgccaaatca cgcgcttcat ctatcccccc
     6961 tccataatag ggaagcactt catctcggaa ataaaaactc atgactgcgc ttactacttt
     7021 atgatctgat gaaatgatca ataactcgca atcctctcca aaagtagatt taagcaatcg
     7081 aaaatatttt tttgagaaaa caggcgtacc taaccgatga acgctagtcg aataagcata
     7141 aaagaaccga tcaatatcag tgtcaatact accttgcaga ttattcttaa taccctttct
     7201 aaccattgcg cgttgcttcc gaggtatagc caacatattt ttctcagcat ctacatcgat
     7261 ttcttttcta aatgttacat agagcccttt ggttacccaa tcagaatggt aaggtgtaat
     7321 attgcgatat tcgagatagt caactgctaa ttgaatggcc aacttctgag ctgcttgatc
     7381 aagcagcttt cttgcctgat cgtctgccgc agcaatacca ccatatacac aaaaagggag
     7441 cgaactcagt gagtggccaa agaggtgact gttcacttct actaatggta atacacctat
     7501 aatttgttga tcacgttcag catagagaaa ccatgttttg tgaccaaatg cattctctat
     7561 tacacttttc caacccgctc gatgaaagaa agtcgcttct ttacaggaga ccacaaaatc
     7621 atcccatagt gaagcaacgt cagggttcaa caaagatatc tttatagaat ctgttaccgc
     7681 aattttattt atagactcaa gtaaagtcgt atcgatagaa ctcatctatt tttttccaag
     7741 aaaatttgat ccattcttcc ccaaccaaaa tcattgagca agttagtcag tcttttctcc
     7801 attaaatcta gatttaagta gtgccggaag cgcactttaa gatttatacc tttttggcga
     7861 ggctgatcag gatctatttc ccaaggatga aaatagaata cagcagaacg ctgatctttc
     7921 tcatttaccc tttgaataaa ccagcgtgaa acagcgtacg gccacaaacg aaagtaccca
     7981 cctcctccca ctggaatatt acgccccaat aaatttactg ttgtaatggg tacttcgagt
     8041 aacttccctg tcgggttggg ataaaaagca aagcggggag catcaggaat accataatga
     8101 tcgtgacgta ctggatagat acttgaacta tagagataac ctgcttcttc aagtaagttt
     8161 aatgcccaat aattctgctt attaatcgag aaacttgggg ctcgataacc aataactggg
     8221 aatccactta tatcttcaag tattttttta ctgtcggcga tatctttata aaaatcatct
     8281 gatcctaact ctgtaacccg atcatgatca cacccatggc ttgctaactc atgaccattc
     8341 tctacaatac gccgaaccat cgagggatag cgtttagcta tccaacccaa agtaaaaaaa
     8401 gtcgcattta tgcctttttt ttcgagcaat gaaagaatcc gatcaatatt ccgctccaca
     8461 cgacaaggga tactatccca atcatttctc ggaatatagg gatcaaacgc tgatacttgg
     8521 aaataatcct caacatcaat cgttaaagca ttacgtatca tattgatctt tccctatcgt
     8581 gtacaacaat caaaagctaa gcaaacctaa tcgattcttg atgtaacaaa ccctttttca
     8641 gggcgccatt gaatactgaa ttaatcactt gtttcaatat tttgaaaagg cggtaaatca
     8701 aactcttgtt gaatgtcggc aacaacctca ttcacttctt gacttgttat cgtgcgtagt
     8761 tcctctaagc atcccattat tagcaatcga tcacatagcg tattaatttt tcgtggaata
     8821 ccacctgtga agttataaat cacatcaaag ctatcatcct gcagtaacgg acttccctgc
     8881 cagcctgcaa tcgccaaacg atgctcaata taagcgcgtg tttcagtatt atccattggg
     8941 ccaagatgat aagtagcgat cactctctgg cgtagttgct gcatgttatt acctaataat
     9001 gtttttctaa attctggttg acctaataga aaggtctgta ataaaagctt atcgtctgtc
     9061 tgaaaattag aaagcattct taattcttca agtgctcgcg atgttaaatt ctgcgcctca
     9121 tcaactaaca ataatatttt tttcccttcc ttatcatatt gcctaagaag ctgctcgatt
     9181 tctaaaagcg tcgttgcttt tgatgtattg gtcactggta atccaaaaga agctgcaacc
     9241 attctcaaca tgtcatctga atcgagattt gtgttagcta gttgcgccac aataaaacgc
     9301 tgctgatcca atttctggaa gagatggcgg atgagtgtgg tttttcctgc accaacttca
     9361 ccggtaatga tgataaaacc atcttcctgt gatatcccat attctaaata agcaatcgcg
     9421 cgcttatggc ctttactact aaaaaagaaa ctagcgtcag gctttaattg aaacggtttt
     9481 gtttttaaac catagaaaga taggtacata aattaaatta gttgaaacaa acatttagaa
     9541 attcattgaa agagatgcca tatagttgtt cgaagaaaaa tcaccattga tccgttctga
     9601 gtctcttcga ttatgaaaat agctaatttc gccaaataga ttaggagaaa atcttttatt
     9661 caaactaacc atcattattt ggttaatatc agttagatta gttgtcgtaa aatttattcg
     9721 agtaaaagtg tagttagttg tgaaattcat gagcggcgac aatttataaa tccaagaaac
     9781 ccctccccct gtttgaaccg tattcctatt tagtacggaa ttgactcttc ctccaatgga
     9841 atcatcttct tcatctccag tataagcctg ccgcgatatg ttaaaccctc taaaaactat
     9901 cgtattacga cttccattga tggtcataaa cgcttgtaag ttccgttgaa ggaataatcg
     9961 attggtaaaa aagttattgg cacccgagat acctaataag gcaccttgat ttgcaagccc
    10021 ccccccaaag ccagcattcg tcattgtatt taatagaagc tgaggcaata tccctccaga
    10081 aggaatagca cctgccgctc caggttgcaa taaattttgt tgcccaaaag tggttatatc
    10141 ttcgttataa gatgcactcc aagtagttaa tcgagtgcga tgactaaaat tagctaaata
    10201 cgtatcacca aaaatccttt gacctccagc aaaatcaaat tccgttcttt cattgggtat
    10261 ccaggaaaaa cccactgtcc aggtagggct actcgtgcct ccattaacgg agataaagct
    10321 atttctttca taccctcccg tacctacaag actaaattgt ggcgttagca tatatcttag
    10381 cagcactgta gatcgctcaa gcgcaatcgt cctaagttga ctccgatgaa tctcagtatg
    10441 attgtaggta ataccccatt gcagatccct gaaagccgta ctactgttca aacgaactgt
    10501 gccattgtct gttgaagagt ctcgcaatcc ccccccactc gtattcactt ctccatgggt
    10561 ataacgtaac tcagcagttg caaaatcacc aaagcgatgt ctatagtatg ggcttatgtt
    10621 taacatactc atatttcttc gattaagtgg gttagcatta tctaatccct gagaaccaag
    10681 cagactggca ttttgctgta aaatcacggc ccgaccatca atgaaaaacc gatcctgaat
    10741 taactcaata gttccattag cattaagctg atgcctaatt ctgttaaatt cactattatt
    10801 cgcatatatc agattattca ttaagtaatt aacgtctact cggtaacgtc gggcatttcc
    10861 attaatgagg atgcttggat taatctgagt gataaattca tcattccctt tacctcctgt
    10921 cgctatacca cccagtctta aattatccgt atagcgttca ttaatattca aactaggtac
    10981 aacgcgccat tctcctgcgt atataggtaa agctataata ttaaatagta aaatactaat
    11041 gtaaattaaa cgcttgcaat gataattgca gaaatgttta gaaagcagct tcatgaggag
    11101 taataataac catagtaacc tgcagccgcc gaagttctgg ccttgttata gattaaatta
    11161 ataacatcac aggattcaat ctgacgcaat gcttccttga cagtctgttg agtagttctt
    11221 tctgcttcca ccaccaaaac aatctgtccc atttgacttg ctaaaacgcg cgcttcacta
    11281 gtaagaagga gtggaggaga atcaaaaatt acaatacgat cgctataacg ttgtgctagc
    11341 tccattaaaa tatctgccat actctgactt gccagtaatt ctgttgcatg gggatgacga
    11401 gttcctgctg caatcaaagt cagcttatca acacttgttt tagtcaaaat atccgatacg
    11461 tttaatctct gatccaaaat aatatctaat aatcctttac gatctggact aaaatccagc
    11521 atttttggaa ttgaggggcg tgctacatca gcatcaacca ataaaactct attatccatt
    11581 tccattgcga tactcatcgc taaatttgta gaacagaagc tcttaccctc acctgccaac
    11641 gaactggtga tcattatcag attaccgttc tttattccag aatttttact aaacgcgttt
    11701 gttaacagtg gacgcttaat aatacgaaac tgttcagcga tttgggtttt tccatgttgt
    11761 ggcgtcacaa tacccaattt atgcaatctg tctagatcaa tatctatgtg aggctgtggc
    11821 agatgagtat caacacttac tttcttacct acagttcttc cctcagtaat agatatcggt
    11881 ttatcaatat actctcggtt tatcttaaca gcattatcta ccccttgtgg ttcaataggc
    11941 tgtattttcg gcgaatcagc tattgtattc ttaaattttt ctgtaattct ttcaatatct
    12001 gaggcctctt tttgaatctt tcttttagag tcttgaatta tatctttatg aatattctta
    12061 attgccggta ttttctgttc tgcatcagca ttactcttta gcttgtcagc agccctttct
    12121 ataatactca tatcaattcc tcactaaaaa tacctggatt cgcaaacaac gtctaaaaga
    12181 gattaattaa tgttgcaact ggaagttttg tgtaaatcat taaggtagta tataaagcca
    12241 tcagtaccaa tagagaaaaa ccgaatgcat agagtcgatt tcttcgctta gatttttctt
    12301 gatcggtcca aaccattgga atggtgccaa gaatagcaag accagtaact tgtcgcaaat
    12361 cagtctgact atggaatgtt ggacgaattt gactaattat aaaagcaacg ccaataccag
    12421 ctagcaacgc gccaataaaa acaaacgagt acattttctt tcgatctggc cctgatggag
    12481 tctcaggaac aacaggcggg tctataatcc taaatgacat taaacccgtt gtagaagtca
    12541 gttcgcctga tatctcagca gaggccctgc gttcaagtaa tttctcataa ttagctttat
    12601 taacattata atcccgactt aattgagtaa gttctgcctc aactgttgga atcatagaac
    12661 tcagcgattt aagtcgctca aaacgattcg tgtattcaga tacccgtgct tgcattgaag
    12721 cgaccaccgc ctcagcttca gtcaatgcaa tattcaattg ctgtagcatg ggactaaaat
    12781 tccttccttg atctaccgca gttggatcta tcagcgttgc ctcttctttt ttacgttctt
    12841 cgagttgagt aattaaacgt ttagttgcca caatgtcggg atgtaaatct gtaaaattga
    12901 gtctaagatt atcaagattc agattcagcg cctgaatacg tgcatcaatt tcagggttaa
    12961 ttattttttc taaaacattt tcatgtgttt caaggagtaa aaccgtttca tcaccactta
    13021 tttgccttct tacagcgtct cttgcttgtt cagcttcttt taatgccact tttgctttct
    13081 caagctcttc tagggcattt gttaactgcg cgtaataatc cccactttga cctggcatta
    13141 ggccgatatt tctttgccta aaccctgtca atgcagtttc agaagcgatg agtttttctt
    13201 cataactcgc tatttgttga tctaaaaaac gtaatgctga agtcgaatcc tgttttttac
    13261 tttctaaacc accttcaaca aaaatagtta gtaatgactg cacaatatct ttagctaatt
    13321 ttggatcttt atggctatat gaaatagtaa aaagattttc cctaccggct gcacccaatc
    13381 taatatcttt cattaatgaa gtaacaagct tgtcgtatgc gacatcatca gtaaccttca
    13441 catccagatc aaccattctg atcacccgtt caacattggg acgactgatc aaagttcgac
    13501 tcatgatcgc aacttgctgc tctggattgg gtgagacagt catacccgcc atcaggggcc
    13561 taagaatatt ttgagtgtcc acatgaattc gcgcggaagc ttcataatca tcaggtaagt
    13621 gatgaacaac cgtccaaccg ataatagata caagccatgc gattgcgatg gaaacccaac
    13681 ggtacttcca ggttccttta atataaacaa gcagttgagc aattaactca tccatcttgc
    13741 atcaatctcc atttctataa aaatcttaac agatgctgtt atgattaaaa aaaactctca
    13801 ggaatcacca aaatatcacc ttgatataca ggagtattag cagttatatc tccatctcta
    13861 atcaagtcat ttaacctaac tctgaattgt tgctgtttac catctatggt tcgaataatt
    13921 cttgctcgat ttcctgctgc aaaatctgtg attccaccaa ctgcaatcat cacgtccatt
    13981 agtgtcatat tttcgctgta aggcaaagct tggggccgtg ttgcttcacc gattactcta
    14041 atttgctcag taaatggccc tacaaatcca gtta
//
LOCUS       DAOQLJ010000051        13646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1665_length_13646_cov_5.984475, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000051 DAOQLJ010000000
VERSION     DAOQLJ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 13646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13646
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1665_length_13646_cov_5.984475"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..678)
                     /locus_tag="PK522_05230"
     CDS             complement(118..678)
                     /locus_tag="PK522_05230"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNC41168.1"
                     /translation="MLFSKRNKAQWIVLIPWFIAAQMSAASASEQDLSRETKAHAASA
                     ARIHCSDNSAYQREPDPELTASIKSMAESTDSSERLVALAQLSEQRAIDPEVADQILY
                     IALLDDDPIIRGQAVYAIGRRGGMDANIVIDQALADEVPSVRLMAVDALVASDEESNA
                     LLRKALEDESAAVRELASTKLGLVAR"
     gene            complement(812..1561)
                     /locus_tag="PK522_05235"
     CDS             complement(812..1561)
                     /locus_tag="PK522_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41169.1"
                     /translation="MQKNSSSKNMSRALIAMGLIIAASQVSAHTRLQVPQIPEGTRVY
                     NNVVVGHGCEGKDVIATTAVFPDGVDSTVLVDGNPYEGSISEFINWGNLIQKVMSKDV
                     FAKEAEKYDQSGSNVVGFWVRDGALPATYTGLFPFRSDAAVIEPSSCAKSVKVAVAIA
                     DICKNTNIGGFTDLTVNLWTPAVGSNFDGPGLHGYNSPATLTINRDLAANPLDASCGE
                     GSAVELLPSAAQLDRDMPIKNKDGGVWWPKN"
     gene            complement(1746..3890)
                     /locus_tag="PK522_05240"
     CDS             complement(1746..3890)
                     /locus_tag="PK522_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41170.1"
                     /translation="MVDLSDIDLTYKLVIGLFNAAPGKANLASLLSAIDDGLSLPQIG
                     DRLDSTLLFNQRIIGDLSEADQVSLIMSHFGFVHGQSTGNERKQVRDYLTGRLKSGDS
                     WGQIVYDAVVYLSGNPDPMFAKAALLLSNKVLVSSLFSQSYSEDSLEVLQSVLSGVSA
                     DSLLDEVSAEAYLAEIGKPVGAVNLTVAKDVLSSHVILAPRGYTPAGTDQINTLNDDD
                     VLSGTASEIDKLVFDFVNDADTGDHNIVPQLSGIEILDIKFSLDGAGTLDLQDTVGVT
                     HIDLHRIDDGATAIIDNITEATTNHLSINRSNVTDSNIAFTYLDSALIGTDDVVTLTL
                     NNAQAGVVSIQESTDDGSLDQGFETINVISSGDQANTLISLTTEDAQLLNITGNQDLT
                     IQALSGTNQSSLVGLQNMGGSLTKIDASTFAGNLRININGLLDAVLDDSSGKDVPVTV
                     LGGIGNDTIWLSSKNNVNDELNGGTGVNTLVLGGGFDSATVTELNPDKLKNIVNFSTL
                     ASTNFSLTPEFLKLQLDDFVLDSLVDSEHISSIEEPETLTLRINDFQDIETPQAAVAL
                     QLDISDLTQQSAVNVTLDTGSPHYQLEHDTIQLSPHGSSITINNFQTLSDGAVVDVFG
                     GSAADQIILSLADFKLVDSSAGNNIAITDFLFAFDTIDPASDPSVEAIIFDSTTGNLF
                     YNPDYATEGGLVLIASLMNISDLTDLDIFILS"
     gene            4369..4803
                     /gene="gspG"
                     /locus_tag="PK522_05245"
     CDS             4369..4803
                     /gene="gspG"
                     /locus_tag="PK522_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNC41171.1"
                     /translation="MKGCYTNLGKRTIQGFTLLELLVVMVIIGLLAAYVGPKYFSQVG
                     KSEVKMAQAQIDSLEKALHQYRLDVGQYPTSEQGLNALVTRPGNELKWQGPYLSKMPP
                     PDPWGRPYQYKYPGDRAEFDLFSLGKDGQPGGTGDAADIANW"
     gene            4815..5999
                     /locus_tag="PK522_05250"
     CDS             4815..5999
                     /locus_tag="PK522_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNC41172.1"
                     /translation="MRYTVKAILANQGTVSLELEAINEEDARQQVLTQGGLVLSVQRH
                     WLDWRPKARVRFPLTHFSQELLSLLTAGLSLVESIETLIEKEEDAGIKKIIENILARL
                     YEGVTFSNAMESHPQAFPALYIATVRASERTGDLPEALTRFITYQNQMDLARKKLVGA
                     SIYPVLLLVVGLLVALFLLLFVVPKFSAIYEDIGADLPWLSVLLIKWGRLVHDNGWML
                     AGIAIASLSLMSYGFSRPAFRAYLMQTLWRIPAIGERMRVYQLARFYKTLGMLLRGGV
                     PITKALEMVSDLLQAHFRPRVRAAASFIREGKTISSAMQTAELTTAVGNRMLRVGERT
                     GLMGEMMERIGNFHDEEISRWVEWATKLIEPILMAVIGTVIGGIIILMYMPIFELAGS
                     IN"
     gene            6011..7693
                     /locus_tag="PK522_05255"
     CDS             6011..7693
                     /locus_tag="PK522_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNC41173.1"
                     /translation="MHSEAVTKPLLDLAQLTQARQQAMARKITVVKVLEDEGGFSPYD
                     LMQELGRLLHMPVLKMEELHTLQPVYEELTFAEAITQQCVLFQRDGKYLLAVGDPFLP
                     NLRSWVEEYISVIAEWYLVHPADLAAFFAQQEKSMRAMDNVLPAEQQSFVQDGIEDLS
                     LKTIHEGTSHVVRLVHSTLYDAHKSQASDIHLETAPGALAIKYRIDGVLTMIGVMQGT
                     DLAEQVISRIKVMSDLDIAERRVPQDGRFKVSIQGREIDFRVSIMPSIFGEDAVLRIL
                     DRQALADHVEGLTLNQLGFDQSAIATIRRLSAEPYGMLLVTGPTGSGKTTSLYAAISE
                     VNQGDDKIITIEDPIEYQLPGVLQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDA
                     ETAQIAIQAALTGHLVFTTVHANNVFDVIGRFSHMGVDPYSFVSALNGIVAQRLVRQL
                     CMHCVVDERPSNEMILESGISLEQAEQYHFRVGRGCGQCRGSGYKGRNAIAEILLLND
                     EIRELIISQATIRRIKEAARQNGTRFLREAALDRVKAGQTSLQEANRVTIVA"
     gene            7674..8528
                     /locus_tag="PK522_05260"
     CDS             7674..8528
                     /locus_tag="PK522_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41174.1"
                     /translation="MSPLWRDRIHIFFAPGRIDLVRIPKGLKAVPQPKISINFEANRD
                     NPLWKIPVEQLTQLLEKENSISSLKGAELSITLSNHFVRYAVVQFQQGIKDAHEFFAY
                     AQFQMREIYGDRIDSWDISMSDWDPKLGGICAALARELIQQLQALTVRFHMKLKQIEP
                     YLTAAFDQWSRAINYQRFWFIVVEPGRLCALLFSNGGWKSIRNQRLLNSVETELLVLL
                     EQEAIIATQREPIELAFIYAPEHTNLQLPLDKGWQFNRIPFDGILAPNHFPAPVGNTS
                     ELPAHAST"
     gene            8515..9051
                     /locus_tag="PK522_05265"
     CDS             8515..9051
                     /locus_tag="PK522_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNC41175.1"
                     /translation="MRRLSLKFPYTGQQTSPLDYGLLLLGLVFLVATVLHLKSIMEEI
                     SYWETRADRFENAQQQKRAPKTSSSRTNQESQQELKQAREIMKQLNLPWEVLFDSLEY
                     TVTKDIALLSVQPSVANQSIRINGEARNLAALTEFVEAMEREAFFENAHILSYKVKQE
                     NPHRPILFSLITSWIDTP"
     gene            9033..9587
                     /locus_tag="PK522_05270"
     CDS             9033..9587
                     /locus_tag="PK522_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41176.1"
                     /translation="MDRYALNNLLSEIRWQTLRLSAVGKLGFGLMVLSVVFFLAAVWP
                     QYETLDQAQAKVTLLQQQSQRDASQPIQLDDNQAIQVFYDSFPNVDSSPYWVKEIDAV
                     AKKRGVELHGSDYRLVSDKGSRLARYEMILPVRGSYPQIRAFIADMLVTIPAVALVDM
                     IIKREDIKSGRLEVRLSLNLYLNQ"
     gene            9590..10207
                     /locus_tag="PK522_05275"
     CDS             9590..10207
                     /locus_tag="PK522_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41177.1"
                     /translation="MKSLALEPTALRKSILGIALLLTIAAAIWVEQEDQDAIDEIQPA
                     LSVKDSSKPSMQRKEASSLAIHPLGQRKFNTEADDIFAATSWEPTRTPRGGDRSLFGG
                     TASSEDDEGDPFTAMMKHAKSKPQPAPAPVAPPLQFKYLGKVIEGKTTRVFLALADKN
                     HVVGIGERIDNQYRIERISDETIEFTYLPLRIKQTLLINQQSGKL"
     gene            10207..12468
                     /locus_tag="PK522_05280"
     CDS             10207..12468
                     /locus_tag="PK522_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HNC41178.1"
                     /translation="MRLIGYLLSIVLVLSGCATFTNRNEAFLEGKQLIAQGQLDAGLK
                     KLEQAAYEEPGSKEIRAVLFRERETIINKTLFEADKLLASDNLDAAEKKYRRVLEIKP
                     NEERARDGLDALNLSRYQSATINRAKEMLDRNDVEGAEAAVRAVLQENPYHEEARLLV
                     KRLNEHIARAEEPVLALVTAFNNPITMEFRDTALKTIFELISKTAGINFIFDKTVQQD
                     SKTSIFVRNNRIEDVLKLLLMTNQLAYKILNDNTLLIYPDTPAKQKDYQELVVRSFHV
                     ANTDVKQMVAMIRGLVKAKDIYVNEKLNLFVIRDTLEAIRLVERLVAINDFPDPEVML
                     DIEVLELSRTRVMELGPKLPQSVTFSGVPGVGDATVAGVAKFSAIGAAQSTSDGLRNF
                     TVNNQVIIDFKKTLTVDDILANPRIRVKNREKAKILLGDKIPIFQASATATGVISQNV
                     TYLDVGLKVEVEPIISFNNEVSVKVALEVSTLGAAESSGTTRAFRIGTRSAETLLSSK
                     DGETQVLAGLIRDNDRKGFAGLAGLIDLPVIGRLFTSHSFNRESTEIILLITPYVMRN
                     ITQPTKLESEFYSGTAAAAGKLPATIRKTAPKSLAITPAGAGSASAAFSAPMLSDDTE
                     AIPNPFAAAAQANPTTPTLSLQAPATVAMDKEFTATIRLMTQNTKLASELELAYDKGM
                     LEMLDGGEKSGSRTLKLGREEPNGMTAVLRFKAVSPNPGSTNIMVQSISTRNEKGESV
                     DVDLPSPAIIEIQ"
     gene            12477..12974
                     /locus_tag="PK522_05285"
     CDS             12477..12974
                     /locus_tag="PK522_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNC41179.1"
                     /translation="MPFLQKAFCRPNQGFTLVEMIVVVALLSILASAAMPFYQMTVKR
                     EKEQELRIALRQIRTAIDLYKQAADEGRVEKKADEVGYPKRLEMLVEGVPNIKDPKKN
                     MIYFMRKLPRDPMFDNPDIPNADTWGKRSYESPPDDPDEGDDVFDVYSRSNKVGLNGI
                     PYNRW"
     gene            12989..13363
                     /locus_tag="PK522_05290"
     CDS             12989..13363
                     /locus_tag="PK522_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNC41180.1"
                     /translation="MQRPNGFTLIELLVVMAVIATLLSIVAPRYFNSIDRSREAVLKQ
                     NLSIMRDAIDKFYSDTGKYPLSLNQLVEERYIRAVPIDPMTESSQTWIEVPPPDPEIE
                     GMYDVRSSSDRQALDGTFYDKW"
     gene            13368..>13646
                     /locus_tag="PK522_05295"
     CDS             13368..>13646
                     /locus_tag="PK522_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNC41181.1"
                     /translation="MPTFSRQHQGYIYLWTLFSISLAGVALAGLGPMWQTAAQREKEI
                     ELLYVGDQFRLAIEAFYNNATSANRSQRYPESLSDLLKDEGSIVTKRYL"
BASE COUNT         3992 a         2966 c         3010 g         3678 t
ORIGIN      
        1 gctaatgagt acaaatttat ttttaaaaat cttcatagtc ctctcctata aattgccact
       61 ataaaaacat cattaacttg catcaatgat gtaccatcta ctgctattct tttcgtttta
      121 cctagcgact aaccccagct tggtcgatgc cagttctctc actgctgcac tttcatcctc
      181 caaggccttc ctgagcagcg cattactctc ttcatcactc gcaacgagtg catcgactgc
      241 catcaatctg acggaaggaa cctcatccgc aagtgcctga tcgatgacga tgttggcatc
      301 cataccaccg cgccgaccaa ttgcataaac tgcctgaccg cgaatgatcg gatcatcgtc
      361 caataaagca atatacaaaa tttgatcagc cacttcagga tcgatagccc gttgttccga
      421 caattgcgca agtgcaacca acctttctga tgaatccgtt gattccgcca ttgatttaat
      481 cgaagcggtg agctcaggat cgggttcacg ttgataagca ctgttatcac tgcaatgaat
      541 gcgggctgcc gaagcagcat gagctttagt ttctctactt aaatcttgct cggaagctga
      601 agcggcgctc atttgggccg cgatgaacca gggaatcaaa actatccatt gtgctttgtt
      661 acgcttcgaa aatagcatcc tgccctcctt acaacatcct gttaaacgag caatcacatc
      721 aaactacaat gtaactgccc gcttgtgcgt tatggttgaa ctgctacgta aacgatgggt
      781 ttacgttaaa aacaataaac taactcgcaa attagttttt tggccaccaa acaccaccat
      841 ctttattctt gattggcatg tcacggtcaa gctgtgcagc cgatggcagc aattcaacgg
      901 ctgaaccttc tccacacgat gcatcgagcg gatttgccgc taaatcacga ttaatggtta
      961 gcgttgcagg tgagttatag ccatgcaaac ccgggccatc gaagtttgag cccactgcag
     1021 gcgtccaaag attaacggtc agatcagtaa aaccaccgat attggtgttt ttgcaaatat
     1081 ctgcaatggc tactgcaact tttacgctct tcgcgcaaga agaaggttca attactgccg
     1141 catcggatct gaaaggaaat aatcctgtat aggttgcggg cagcgcgcca tctctaaccc
     1201 agaaaccgac gacattactg cctgattgat catatttttc ggcttcttta gcaaaaacat
     1261 ctttactcat caccttctga atcagattac cccagttgat gaattcgcta atcgatcctt
     1321 cataagggtt gccatcaacc aacaccgttg aatcaacgcc gtcaggaaac actgccgttg
     1381 ttgcaatgac atctttccct tcacaaccgt gaccaacgac aacattgttg taaactcgcg
     1441 ttccttcagg aatttgtggg acttgtaagc gtgtatgcgc tgacacttga ctcgctgcga
     1501 tgattagtcc cattgcgata agtgcacgac tcatattttt tgatgagcta tttttctgca
     1561 ttgattactc ctctttgaag ttcaaatttt tgataaggct aaacttttta cttattaatg
     1621 aactatcgat agtcgctggc agtatcttct aatatccatt agaaaacaat gtgacatatt
     1681 gtcgcaccca taggcatgta cgcattatgg ttattaacta agcatatttt ttgggaactt
     1741 tctgcctaag aaagaataaa aatatcgaga tcagtcaaat cagagatatt cattaaacta
     1801 gcaatcagca ctaacccgcc ttcggttgca tagtcagggt tataaaagag attgcctgtc
     1861 gttgaatcaa agataatggc ttcgacagag ggatctgagg cggggtcgat agtatcgaac
     1921 gcaaataaaa aatcggtgat tgcaatgtta ttgccagcac tcgaatctac caacttaaaa
     1981 tcagcaagcg ataaaatgat ttgatctgcg gcagatccac caaaaacatc gacgaccgcc
     2041 ccgtcggaaa gcgtctggaa gttattgatg gtaatactgc ttccgtgtgg cgataactga
     2101 atcgtatcgt gttcgagctg atagtggggc gatcctgtat caagcgtcac gttgaccgcc
     2161 gattgctggg ttaaatctga aatatctaac tgcaaagcaa ccgcagcttg aggtgtttca
     2221 atatcctgaa aatcattgat gcgtagggta agggtctctg gctcctcgat actactgata
     2281 tgttcgctgt caaccaatga atccagcaca aaatcatcga gctgcaattt taaaaactca
     2341 ggcgttaggg aaaaattagt gctcgccaag gtactaaaat taacaatatt ttttagctta
     2401 tccgggttaa gctcggttac tgttgccgaa tcaaaaccgc cccctagaac cagggtatta
     2461 accccagttc ctccatttaa ttcatcattg acattgtttt tactgctcaa ccaaatcgta
     2521 tcatttccta ttccaccgag tactgtaact ggcacatctt tacccgaaga atcatccaag
     2581 accgcatcca gcaaaccatt aatattgatt ctcaaatttc ctgcgaatgt tgatgcatcg
     2641 attttggtga gcgacccgcc catattttga agccccacca atgaggattg gttagttccg
     2701 ctcaatgctt gaatagtcag gtcctggtta cctgtaatgt tgagtagttg cgcatcctca
     2761 gtcgtcaaac taatcagtgt attagcttga tctccggaag agatcacgtt aatcgtttca
     2821 aaaccctgat ccaaagaacc atcatcagtc gattcttgaa ttgagaccac gccagcttgg
     2881 gcattgttaa gtgttaacgt tacgacatca tcagtaccga tgagtgcaga atcaagataa
     2941 gtaaacgcaa tgttcgaatc ggtaacattt gaacggttaa tggataaatg attcgtcgtt
     3001 gcttcggtaa tattatcaat aatcgcggta gcaccatcat cgatgcggtg caaatcgata
     3061 tgagtaacac caacggtatc ttgtaaatca agcgttcctg cgccatcaag cgagaacttt
     3121 atgtcgagaa tttcgatacc acttaactgc ggcacgatat tatgatcgcc cgtatccgca
     3181 tcattgacaa aatcaaatac tagcttatca atttcggaag cggttccgga taacacatcg
     3241 tcgtcattca atgtattgat ttgatccgtt cctgcaggcg tatagcccct tggagcgagt
     3301 atgacatgac tactgagcac atcctttgct accgtcaggt tgacagcacc gactggttta
     3361 ccgatttcgg ctagatatgc ctcggctgat acttcgtcta ataggctgtc cgcagataca
     3421 cccgatagca cgctttgtag cacttctaag ctatcttcag aataagattg tgaaaaaagg
     3481 cttgaaacta gcaccttgtt gctgagcaac aaagcagctt tggcaaacat tggatccgga
     3541 ttaccggaca agtagacaac tgcgtcataa acgatctgcc cccaactatc ccccgatttc
     3601 aagcggccag tgaggtaatc tctgacctgt tttctttcat tgccggttga ttggccatga
     3661 acgaatccaa agtgactcat gatcaaagaa acttgatcag cctcagataa atcgccaatg
     3721 attctttggt taaatagtaa tgtcgaatct aatcgatcgc caatttgagg caatgataat
     3781 ccgtcgtcaa ttgcgctgag caaacttgcc agatttgcct tacctggggc tgcattaaat
     3841 aatccaatta ctaacttata agttaaatcg atatctgata aatccaccat tcgtgatgat
     3901 tttctctcaa attattattg ttattcgttt tcgtaggaca aagacgccct cagagcaggg
     3961 gctttgtagt ttggattcgg caataataac gagtcgtaat cagaacaatc aatgtgacat
     4021 attgtcgcac cgtagctaac cgctgatcag tgctgtcttt gaacgatcgc agcgatcaga
     4081 tagatcgtta acttaataaa aatgtctaat atcaatacta taattaaaaa aagaaatgga
     4141 acgccgttga ttcaagcccc aagattctgt ttggtgataa tgacaaatcg cacggttagc
     4201 cgatttgagc ggttggaatt cgcgagatta atatgttatc gtaaaaaaaa gattgtcaag
     4261 accaagtaaa aagtcgtgaa aaagtttagt gttgcttaat ttgcgatggg ccttgaatct
     4321 actgatattt atgggttatt aggaaagttt tatacgtcga ggataagcat gaaaggctgt
     4381 tatacgaatt taggcaaacg cacaatacaa gggtttacct tgcttgagct tttggttgtt
     4441 atggtgatta ttggattact agcagcttat gttgggccga aatatttttc acaagtgggc
     4501 aaatctgaag tcaagatggc tcaggctcaa attgattcgc tggaaaaagc gctgcatcaa
     4561 tatcgcttag atgttggtca gtatcccacg agtgagcaag gactcaatgc gttagttacc
     4621 cgcccgggca acgaattgaa atggcaagga ccttatttaa gcaaaatgcc gccacctgat
     4681 ccttggggac gcccttatca gtataaatat ccaggtgatc gtgcagaatt cgatttgttt
     4741 tctctaggaa aagatggtca acccggcgga acaggggacg cggctgatat cgcaaattgg
     4801 taatatttta gttaatgcgt tacacagtca aggccattct tgccaatcag ggcacggtat
     4861 cgcttgagct tgaagcaatc aacgaagaag atgcgcgcca acaagtcttg acacaaggtg
     4921 gattggtatt aagcgtacag cgccattggc tcgattggcg gcctaaagcg cgtgtacgct
     4981 ttcccctgac gcattttagc caagaactat tatcgttgct cactgctgga ctgagtttag
     5041 tagaaagtat cgagacattg atcgaaaaag aggaagatgc tggcatcaaa aagattatcg
     5101 aaaatatcct tgctcgactc tatgaaggag taacattctc gaatgcgatg gaatcccatc
     5161 ctcaagcctt cccagcgcta tatatcgcaa ctgtacgggc aagcgagcga acgggcgatt
     5221 tacccgaagc cttaacacgg tttatcacgt atcagaatca aatggatcta gctcgcaaaa
     5281 agctggtggg tgcatcgatc taccccgttt tactattggt tgttgggcta ctggttgcgc
     5341 tattcttgct gttgtttgtc gtccctaaat ttagtgcaat ttatgaggac attggtgcgg
     5401 atttaccttg gctttcagta ttactgatca aatggggacg attagtgcat gacaatggct
     5461 ggatgcttgc gggtattgcg atagccagct tatcactcat gtcgtacggt ttttcaagac
     5521 ccgcattccg tgcctatctg atgcaaacat tatggcgaat ccccgcaatt ggtgaacgca
     5581 tgcgggttta ccaacttgcc cgattctata aaacactcgg tatgctatta cgcggggggg
     5641 tgccgattac gaaagcactg gaaatggtaa gcgatttatt gcaggcgcac ttcaggcctc
     5701 gtgttcgtgc agcagcaagt tttattcgag aaggaaaaac catatcaagt gcaatgcaaa
     5761 cggctgaact aaccactgcg gtaggaaatc gaatgctccg cgttggcgag cgcacgggtc
     5821 tcatgggcga gatgatggag cggattggaa attttcacga tgaagaaatt tcacgctggg
     5881 tcgaatgggc aaccaaactc attgaaccaa tattaatggc ggttatcgga acggtgatcg
     5941 ggggcatcat catcttgatg tatatgccta tttttgaatt ggcgggcagc atcaattgat
     6001 tgaatccgcc atgcattcag aagcagtaac aaaaccattg cttgatttag cacaattaac
     6061 gcaagcccgc cagcaagcaa tggctaggaa aattaccgtt gtgaaggtac tggaagatga
     6121 aggtggattt tcaccctacg acttgatgca ggagcttggg cggttgcttc acatgccggt
     6181 tctgaaaatg gaagaattac atacactgca accggtgtat gaggaattaa cttttgctga
     6241 agccatcaca cagcaatgtg tcttattcca gcgcgatggg aaatatctac tggccgttgg
     6301 tgatcccttc ttacccaatt taagatcttg ggtagaagag tatatttctg ttatcgccga
     6361 atggtactta gtgcaccccg ctgatctggc tgcgtttttt gcgcaacaag aaaaaagtat
     6421 gcgcgccatg gacaatgtct tgccagcaga acaacaaagc tttgtccagg atggtatcga
     6481 agatttatcc ttaaaaacga tacacgaagg aaccagtcat gtggtcagac tcgttcattc
     6541 cactctttat gacgcccata aatctcaggc tagtgatatt cacctcgaaa ctgcacctgg
     6601 cgccttggca attaagtatc gaatcgatgg tgttctgacc atgatcggtg taatgcaagg
     6661 aaccgactta gctgaacaag tgatctctcg catcaaagtc atgtcggatc tcgatattgc
     6721 agaacggcgc gttccacaag atgggcgctt taaagtttcc attcaaggac gtgaaattga
     6781 ttttcgtgtt tcgatcatgc ccagtatttt tggtgaagat gccgtactaa ggattttaga
     6841 ccgacaagcc ctcgcagatc atgtcgaagg cttaacgttg aatcaactgg ggtttgatca
     6901 atctgccatc gctacgataa gacgtttaag tgctgaaccc tacggtatgc tactagttac
     6961 gggaccaacg ggaagcggaa aaacaacctc actttatgcc gcaatctcag aagtcaatca
     7021 aggggatgat aaaatcatta ccattgaaga ccccatcgaa tatcagctac ccggtgtatt
     7081 gcaaattccc gtcaatgaaa aaaaaggact aacgtttgca aggggcttgc gttctatact
     7141 caggcatgat cccgacaaga tcatggtcgg tgaaattcga gatgctgaaa cagctcaaat
     7201 tgcaatacaa gctgcgctga cagggcatct cgtttttacc acagtgcatg ctaataatgt
     7261 gttcgatgtg attggtcgat tttcacacat gggcgttgat ccttatagct ttgtctcggc
     7321 gttaaatggc attgtagcgc aacgactagt gcgccagctt tgcatgcatt gtgtggttga
     7381 tgaacgaccg agtaacgaga tgattcttga atcgggtatc tcgctggaac aagctgagca
     7441 atatcatttc agggtcggca gaggttgtgg gcaatgtcgt ggcagtggct ataaaggtag
     7501 aaatgcgatt gctgaaatat tgttattgaa tgatgaaatc cgtgagctga ttatttcgca
     7561 ggctaccatt cgccgaatta aagaagctgc aagacaaaat ggaacccgct ttctaagaga
     7621 agccgccttg gatcgagtca aagcaggaca aacgagctta caggaggcta atcgtgtcac
     7681 cattgtggcg tgatcgaatt cacattttct ttgcgccagg caggattgat ttagtcagaa
     7741 ttcccaaggg gcttaaagct gtgccacagc ccaaaatttc aattaacttt gaagcaaatc
     7801 gggataaccc gctgtggaaa attccagttg aacagttgac acagctactc gaaaaagaaa
     7861 atagcatcag cagtttgaag ggtgctgaac tctcgatcac tttgtccaac catttcgttc
     7921 gctatgctgt tgttcagttt cagcaaggta tcaaagatgc tcacgaattc tttgcttatg
     7981 cgcaattcca gatgcgagaa atctatggcg accgcatcga tagttgggat atttcaatga
     8041 gtgactggga tcctaaatta ggcggtattt gcgccgcatt agcacgcgag cttattcagc
     8101 aactgcaagc actaacggta cgattccata tgaaactcaa acagattgag ccttacttaa
     8161 cagccgcatt tgatcaatgg agtcgtgcaa tcaattatca gcgattttgg tttattgtcg
     8221 ttgaaccagg tcgcctatgt gcactgctat tctcgaatgg aggttggaaa agtattcgca
     8281 atcaacgatt attgaattca gtcgaaaccg agttactggt attactcgaa caggaagcaa
     8341 tcattgccac tcaacgagaa cccattgaat tggctttcat ctatgcgcca gaacatacta
     8401 atttacaatt acccttagat aagggatggc agtttaatcg gataccgttt gacggcattc
     8461 ttgctcctaa tcattttccg gccccagtgg gtaataccag cgagcttcca gcgcatgcgt
     8521 cgacttagcc ttaaatttcc ttataccgga cagcaaacga gcccacttga ttatggcttg
     8581 ctgctgctgg ggctagtgtt tttagttgct accgttttgc atttaaaatc gatcatggaa
     8641 gaaatttcgt actgggaaac ccgagcagat cgctttgaga atgcgcaaca gcaaaagcgt
     8701 gccccaaaaa catcaagctc acggaccaac caagaatcac agcaagaact caaacaagcc
     8761 cgagaaataa tgaagcagct caatttaccc tgggaagtat tgtttgattc acttgaatat
     8821 acggtaacaa aagatattgc gctgttatcg gtgcaaccca gcgtagccaa tcaatcgatt
     8881 cggattaatg gggaagccag aaatctagct gctttaactg agtttgtcga agccatggaa
     8941 cgtgaagcat ttttcgaaaa tgcacatata ctcagttata aagtaaagca ggaaaatccg
     9001 catcgaccta tcctattttc tttgattacc tcatggatcg atacgcctta aacaatttat
     9061 tgagtgagat tcgctggcaa accttgcgtt taagcgcagt cggtaagctt ggatttggct
     9121 tgatggtgct ttccgtcgtt ttttttctag ctgcagtatg gccacaatat gaaacgctcg
     9181 atcaagctca agcgaaagtg accttgctgc aacaacaatc tcaacgagac gcttcacaac
     9241 caatccagct tgatgataat caggccatcc aagtttttta tgattccttt cccaatgttg
     9301 attcatcgcc ttattgggtt aaagaaatcg acgctgtggc caagaagcgg ggtgtcgaac
     9361 ttcatgggag tgactatcga cttgtttctg acaaaggatc gagacttgct cgttatgaaa
     9421 tgatattacc ggtgcgcggc agctatccgc aaattcgtgc atttattgca gatatgttag
     9481 taaccatacc tgctgttgct ttagttgata tgatcataaa acgcgaagat atcaaatcgg
     9541 gtcgattaga agtacgactt agcctaaatc tgtatctcaa tcaataatca tgaaaagcct
     9601 agcgttagaa ccgacggctc tccgaaaatc gatattaggt attgcacttc ttctaacgat
     9661 agcggcggca atttgggttg agcaggaaga ccaagacgcc attgatgaga ttcaaccggc
     9721 tttatcggta aaagattcat caaaaccatc aatgcaaaga aaagaagcta gctccctagc
     9781 cattcaccca ctgggtcaga gaaaatttaa tacagaggca gacgatattt ttgcagccac
     9841 gtcttgggaa ccgacaagaa ccccacgcgg tggtgatcgt tccttatttg gtggcacggc
     9901 atcatccgaa gatgatgagg gagatccctt cacagcgatg atgaaacatg caaaatcaaa
     9961 gcctcagcca gcgcctgcac ccgttgcgcc gccactccaa ttcaaatatc ttggaaaagt
    10021 catcgaggga aaaacaaccc gtgtgtttct agcactagcg gataagaacc atgtagtagg
    10081 aattggcgaa cgtatcgaca atcagtatcg tatcgagcga attagcgatg aaacgattga
    10141 atttacttat ttgccgctgc gcattaaaca aacgcttttg atcaatcaac aatctggaaa
    10201 gctttaatga gattaattgg gtatttattg agtatcgttt tagtattaag cggttgtgca
    10261 acttttacta atcgcaatga agctttctta gaaggaaagc agttaatcgc tcaaggtcag
    10321 ttagatgccg gattaaaaaa attagaacaa gccgcatatg aagaacccgg tagtaaggaa
    10381 atcagagctg ttcttttccg tgaacgtgaa acgatcatca ataaaaccct gtttgaggcg
    10441 gacaaattac tcgcgtcgga taacttagat gcagcagaaa aaaaatatcg tagggtgtta
    10501 gaaatcaaac ccaatgaaga acgtgcacga gatggactcg atgcgctgaa tctttcacgt
    10561 tatcaatcag ctaccatcaa tcgagcaaaa gaaatgctgg atcgcaatga tgtagaaggt
    10621 gcagaagctg cggttagggc tgtattgcag gaaaacccct accatgaaga agcccgtctg
    10681 ctcgttaagc gacttaatga acatattgcg cgcgcagaag agcctgttct tgccctagtc
    10741 acagcattta ataatccaat cactatggag ttccgcgata cagcgcttaa aacaattttt
    10801 gaactgattt caaagacggc tggaatcaat tttatttttg ataaaacggt acaacaagac
    10861 tcaaaaacct caatttttgt tagaaataat cgaattgaag atgtattgaa gctattgctg
    10921 atgaccaatc agcttgctta taaaatattg aacgataata cgctgttgat ttaccccgat
    10981 acaccggcca agcagaaaga ttatcaagaa ttagttgttc gcagctttca tgttgccaat
    11041 accgatgtaa agcaaatggt cgcgatgatc cgagggttag ttaaagcaaa agatatctat
    11101 gtcaacgaaa aactcaatct ttttgtaata cgagatacgt tagaagcgat tcgcctcgta
    11161 gaacgactcg ttgcaatcaa tgatttccct gatcccgaag tgatgctcga tattgaagta
    11221 ctagagctta gtcgtactcg cgtcatggaa ttgggaccta aactacctca atcagttacg
    11281 tttagcggcg tacctggcgt cggtgatgcc actgtagcag gtgttgcaaa atttagtgcc
    11341 attggtgctg cacagtcgac aagcgacggg cttagaaatt ttacagtaaa caaccaagtc
    11401 attatagact ttaaaaagac tctaactgtc gatgatattc tggctaatcc tcgaattcgc
    11461 gtgaaaaatc gtgagaaagc caaaatcctg ctaggggata aaattccaat tttccaagcc
    11521 agcgcgaccg caacgggtgt catttctcag aatgtaactt atctggatgt aggattaaaa
    11581 gtcgaagttg agccgatcat ttcatttaat aatgaggttt cagtcaaagt cgccttggaa
    11641 gtcagtacct taggtgccgc tgaatcatct ggaacaacca gagcctttag aatcgggacg
    11701 cgcagtgcgg aaacgttgtt atcatcaaaa gacggtgaaa cccaagtatt agcgggattg
    11761 atacgcgata atgatcggaa agggtttgca ggtctcgcag ggctaatcga cctacctgtc
    11821 ataggaagac tctttacgag tcatagcttc aatcgagaaa gtacggaaat catcttgctc
    11881 attaccccgt atgtgatgcg taatatcacc caacccacca aacttgaaag cgagttttac
    11941 tcaggtacgg cagcggcagc aggaaaattg ccagccacga tacgaaaaac agcgcccaaa
    12001 tcgctagcaa taactcctgc gggagcaggc tcagcttcag ctgctttcag tgcaccgatg
    12061 ctgtctgacg ataccgaagc aattcctaat ccatttgcag cagcggcgca agccaacccg
    12121 accacgccaa cgttgagtct acaagctccc gcgacagttg cgatggataa agagtttaca
    12181 gcgacgattc gcctaatgac ccaaaatact aaactagcga gtgaacttga actggcctac
    12241 gataaaggga tgctagagat gctggatggt ggagaaaaat ctggatcgcg cacactgaaa
    12301 ttaggtagag aagaaccgaa tggaatgact gcagtcttgc gatttaaagc ggtcagccca
    12361 aatcctggtt cgacgaatat catggtacaa agtatttcaa cccgcaatga aaaaggagaa
    12421 tcagtcgatg tagacttgcc atcacctgca attattgaaa ttcagtagca ggcatgatgc
    12481 cttttttaca aaaagctttt tgtcgaccaa accaagggtt tacgctagtc gaaatgatag
    12541 tggtcgttgc tttactctct atcctagctt ccgctgcaat gcctttttac caaatgaccg
    12601 tgaaacggga gaaagaacaa gaattacgta ttgctttaag gcaaattcga actgccatcg
    12661 atttgtacaa gcaagcagcc gatgaaggta gagtagagaa aaaagcggat gaagtagggt
    12721 acccgaaacg gctagagatg cttgtcgaag gcgttcctaa tattaaagac cctaagaaaa
    12781 atatgattta cttcatgcgt aagttaccac gtgacccgat gtttgataat ccagatatcc
    12841 caaatgctga cacttggggt aaacgcagtt atgaaagccc ccctgacgat ccagatgaag
    12901 gtgatgatgt atttgatgtt tactcccggt ctaataaagt tggattgaat ggaataccct
    12961 acaatcgatg gtaataagga aaactagcat gcagcgaccc aatggtttta cattgattga
    13021 gttactggtc gtcatggccg tcatcgctac tttattgagc attgtcgctc ccagatattt
    13081 caattcgatc gatcgttcac gagaagccgt tcttaagcag aatctaagta ttatgcgcga
    13141 tgcgatcgat aaattttatt ccgataccgg gaaataccca ttaagcctca atcaattagt
    13201 tgaagaacgc tacattcgcg cagttcccat cgatccaatg accgaaagtt cacaaacttg
    13261 gattgaggtg cctccccctg atcctgaaat tgaaggcatg tatgatgtcc gcagtagctc
    13321 agatcgccaa gctttagatg gaacattcta cgataagtgg taatagtgtg cccacctttt
    13381 ctcgtcagca tcaaggctac atttatttat ggacactttt ttcaatttcg ctcgcgggtg
    13441 ttgcattagc aggattaggg ccaatgtggc aaaccgctgc tcaaagagaa aaagaaatcg
    13501 aacttcttta tgtgggtgat caatttcgcc tagcaattga agctttttat aataacgcta
    13561 ccagcgctaa tcgaagtcaa cgctaccccg aatcattatc tgatttatta aaagatgaag
    13621 ggtctatcgt tacaaaacgg tatcta
//
LOCUS       DAOQLJ010000052        13291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1739_length_13291_cov_6.709127, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000052 DAOQLJ010000000
VERSION     DAOQLJ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 13291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13291
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1739_length_13291_cov_6.709127"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..3654)
                     /locus_tag="PK522_05300"
     CDS             complement(118..3654)
                     /locus_tag="PK522_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC41182.1"
                     /translation="MTYSQSTSTQFSSTVMAPELKFGVSFLELYRRDGLLKLDQAFLE
                     FLGSADPVLRARLDQARLSPNEIAPKDESILLIDLAPWAEDFIGQLFGIEKEVRALAA
                     KHHELAPLYFCKRQFVQRRAKTKVSDEILSTIDGIALEKALEVEFGEPFSELVFATHV
                     ANWLDDEANNAARIEKALHYAAWALRTPEGQMHTQAGILFKTPAKLDFLHLLSLSTDN
                     EKGFPLHRLDHLRHREGFSLTDTGTDLVGALDEANYCIWCHEQGKDSCSKGFIQKPKS
                     SEELPSFKKSELGVLLAGCPLEERISEFHKLKTQGHAIGSLAMIVLDNPMCAGTGHRI
                     CNDCMKSCIYQKQVPVNIPQAETRTLKDVLELPWGFEIYSLLTRWNPLDLRRPLPKPA
                     TGKKVLVVGMGPAGYTLAHHLMNDGHTVVGIDGLKIEPLPKEMSGIDLNGTRVPFSAI
                     YDSNSLRVDLNERMPGGFGGVAEYGITVRWDKNFLQFIRLLLERRNEFALFGGVRFGG
                     TLTADDALNLGFDHIALAAGAGRPTVLDLPNGLARGVRAASDFLMALQLTGAAQTDSI
                     ANMQLRLPVVVIGGGLTAIDTATESLAYYPIQVEKFLQRYETLAAVQGEDSIQRSWDE
                     EEREIATEFLMHARAIRAERLQAQKEGRDANIIKLLQAWGGATLAYRKRLVDSPSYTL
                     NHEEVEKALEEGIWFAEGLTPVRIELDQWEHTRSIRFSVQSMDENGKWHQSKEVELPA
                     RAILVAAGTQPNTVLAREDEKYFKLDGRYFAACDERGNPVSITKGNPKPETPAVLLSR
                     CSDGRFISFFGDLHPSYSGNVVKAMASAKQGYSVVSGVLSQVKPVNPQVDHEFIDNLN
                     RQLRAAVYKVERLTPNIVEVIVHAPLAAKHFHPGQFYRFQNFATLSKIAVNTRLAMEG
                     IALTGASVDNSKGLVSLIALEMGGSADLCTLLNPGDPVILMGPTGTPTEIFQNETVVL
                     VGGGLGNAVLFSIGAAARAAGCKVLYFAGYKKLVDRYKVAEIEAAADTIVWCCDEQPG
                     FEPSRQGDFSFVGNIVQAMVAYGSGALGSQSISLKDADRIIAIGSDRMMAAVGIARHQ
                     QLKPYLKDSHFAIGSINSPMQCMMKEICAQCLQPHKDPKTGETTYVFSCFNQDQELDS
                     VDFGGLASRLKQNSVQEKLTRHWISHCLQHLN"
     gene            complement(3799..5070)
                     /gene="cysN"
                     /locus_tag="PK522_05305"
     CDS             complement(3799..5070)
                     /gene="cysN"
                     /locus_tag="PK522_05305"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HNC41183.1"
                     /translation="MSVVENVGLDRAELLRFIAAGSVDDGKSTLIGRLLHDSKSIFED
                     QLSSIAKTSSKRGMQNVDLSLLTDGLQAEREQGITIDVAYRYFATPKRKFIIADTPGH
                     EQYTRNMVTGASTANLAIILIDARKGVLTQSRRHTYLASLVGIPHIVVAVNKMDLVEY
                     SQEVFTQICDDFAVFSQHLNLRNIEYIPMSALNGDMVVERGDQLDWYQGRTLIDLLEN
                     VDIEHDVNLDDFRFPVQWVCRPQTEELHDFRGYMGRVESGSIKVGDAVTIQPSGLHSR
                     IKEILLYQSNFDEAFAPQSVTVTIEDHLDISRGDMLIKSDDQPRVDKEFTAMLCWLSE
                     QPLSSGRKYIVKHTTRMVKGLVSQIDYRVDVNTLNQEKTDSLRMNDIARVVMKVQQPL
                     VFDAYSRNRATGSFILIDETTNNTVAAGMIC"
     gene            complement(5131..6036)
                     /gene="cysD"
                     /locus_tag="PK522_05310"
     CDS             complement(5131..6036)
                     /gene="cysD"
                     /locus_tag="PK522_05310"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNC41184.1"
                     /translation="MNHRSFTHLDALESEAIHIMREVAAECANPALLFSGGKDSIVLL
                     RLAEKAFRPGRFPFPLLHIDTEHNFPEVIAFRDHRAKELGERLIVRSMDDSIKKGRVV
                     LRSENQSRNPFQSITLLDAIAEFSFDACIGGARRDEEKARAKERIFSFRDEFGQWDPK
                     NQRPELWSLYNTKTHSGENIRVFPISNWTELDVWEYIARENLEVPSIYFAHWREVIPR
                     DSGLLPVSHLVQPRDNEKIEKIMVRFRTVGDMSCTCPVASDAASVGEIIAETAMTRIT
                     ERGATRMDDQTSDASMELRKKEGYF"
     gene            complement(6071..6772)
                     /locus_tag="PK522_05315"
     CDS             complement(6071..6772)
                     /locus_tag="PK522_05315"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNC41185.1"
                     /translation="MTEINIEHKVEEVSRLLEDIHENFMPAVFANSFGAEDMLLTDLI
                     ARYFPNIEIFTLDTGRLPEETYALIQTVRDQYHINIKAYFPEAAAVESYIAQNGPNGF
                     YQSVELRKRCCYIRKVEPLRRALTGKRAWVTGMRREQAITRDSLSISQFDAEHAIQKF
                     NPLCDWSNAEVWSYLKQHDVPYNKLHDHFYPSIGCAPCTRAITPGEDIRSGRWWWENP
                     DSKECGLHVSRAVSA"
     gene            complement(6801..8897)
                     /locus_tag="PK522_05320"
     CDS             complement(6801..8897)
                     /locus_tag="PK522_05320"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNC41186.1"
                     /translation="MDYLTDLANDEEIDSYNQSLQDFFAQKMDVDRFTATRLQMGIYG
                     QRQDGVNMVRIKVPGGRLNISQLDAIADVIEKHVQSGEAHVTTRQDIQLHYVPLADTP
                     AVIRHLAKGGLTTREACGNTIRNVTACPLTGVCSREHTDISQHLGGVVRHFLRKPLNQ
                     LMPRKFKMSFSGCEADCAQAMIHDLGVVAVQQGDRFGFKVLVGGGLGHKPHEAIVIES
                     FAEEKDLLLIVEAVLTMHNKYSDRNKRAKARIKFLVDKFGVDGFVEKYREELARVKAV
                     LADQDYPKGDWALGNRASEVPGPGAPRKLFAQKQAGLFVVPISVPLGQVTAKQVRGIA
                     KLLRQHDLKEIRTTQDQNLMLVDVPEAKITDIQATLNELGLSLPKAGDDVVACPGTST
                     CRLGITSSPTVGAKLTGGKDDLRIRVSGCHNGCAQPESGDIGIYGEGKRMYGKLVPHY
                     QMYFGGNGMAEGGLAFKGPSVPSARVEVAVDRVQSAYATNRQNGESFFNWTRRVGQGY
                     FTELLADVIEVKQEDLHAVLRDHGQGDDFRVLQLGGGECAGAKQLQIGTAFFDAANER
                     NYRNALKFQRKLEDSLKCAEEIARLISQGLAQLVGGKKFENLSEQAEELKRVLPTKPN
                     LAVGLAKFAQAFSTPAEELTDSALTEWYAALDEWTAEVADFCIDFDRQLDLAGALPKA
                     VSSIPMKHAQQSTTAL"
     gene            9198..10148
                     /gene="cysB"
                     /locus_tag="PK522_05325"
     CDS             9198..10148
                     /gene="cysB"
                     /locus_tag="PK522_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HNC41187.1"
                     /translation="MRLQQLRLLCEINRQNLNLSKAAESLHTSQPGISKQIQLLEEEL
                     GVDIFVRNGKRIVKITPPGKAIIEIAERMLQDAENLKSVGQEFNNQTSGTLTIATTHT
                     QARYALPPVIKRFTERYPNVKLTLRQGNPTQIATLVSSGEADLAIATEAIELFHDLIM
                     LPCYQWNRCIVVPPNHPLLDSPILTLEAIAQYPIVTYDFAFTGRSKINRAFEANGLNP
                     NVVLTAIDSDVIKTYVELGLGIGILANMAFDPLRDQHLRSIDASHLFEPSTTRVGISR
                     NSYIRGYSYDFIEMFAPHLTHDVVKSAMKGKPNKTEPFSF"
     gene            10520..11602
                     /locus_tag="PK522_05330"
     CDS             10520..11602
                     /locus_tag="PK522_05330"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41188.1"
                     /translation="MEKLNIAYTCNVRKPGMEDERYGEFESPETIEAVISALEGAGAN
                     VELIDVGPDIFQQLEKRKNSIDIVFNNTEGLNEKELREAIVPFFCEFLSIPYTGSSPK
                     TFINKMDKATAKRIVAYDGVPTARFQLMKPQDRLRDLAFPLMVKPYSEGTSIGINQKS
                     KVNNLAELEQAIETIFTQFNQPALVEEFLDGTEYTIGFIGNYLLPILEIDFAKIPGQP
                     QLRDPYVKSIENPFISHLPWNEKARSFASLSIKAHEALEVRDYNRMDFRERNGQLYFL
                     EANVIPGLHPTEADLTNMCRHAGVAHADMVVLILSAAVKRLSEQYPERFKKKTELLTE
                     LANKAICKATHAGEIVYQDRVYNLLG"
     gene            11623..12078
                     /locus_tag="PK522_05335"
     CDS             11623..12078
                     /locus_tag="PK522_05335"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC41189.1"
                     /translation="MIVPISQEMHPQLIKIALQEMGAMYQPELHEELDDIERGRCFGL
                     CKLENGKPVGYVVAKRTIDAYHLLTIAVDIEHRGKGYGSLLIDSIFAEIEKQGGRILN
                     VITDAEANESIRFYLKNSFVLAGIVQDEFISGVAQVHLTKRAGYSPEQY"
     gene            12134..12970
                     /gene="dapF"
                     /locus_tag="PK522_05340"
     CDS             12134..12970
                     /gene="dapF"
                     /locus_tag="PK522_05340"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNC41190.1"
                     /translation="MRIRATKMDGCGNSFLVIDEIKNPLARFDRTKISNALAVQHNTD
                     GILYLDQQHGMPTMKIFDRDGTEETMCGNGLRCATRYFRDQYISSNIFPIVTGDGIKE
                     VTVDNELVEVNLGSARDYCQVSDKMHFVFTGVPHLVVIGNEMSLESAKTLGKLLREDK
                     SLCARVNHPEGINVNFIWRTIEGIAVRTYEVGVEDITLSCGTGSAASAYVAAKVYDLS
                     LPITTQSLGGNLEISDIGGELSIRGITRYQGKVIGFVNHAHPKSTIPSRYWQRSNVSL
                     SL"
     gene            12996..>13291
                     /locus_tag="PK522_05345"
     CDS             12996..>13291
                     /locus_tag="PK522_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41191.1"
                     /translation="MEFSIVTFLLIMVAMAFADVCWTLYFIDVEERKAHPAAFWSAMI
                     ILVTAFSVTNYVENKIYIAAAFIGAYIGTYGTLKWKLHKEKHHAQSESSQTKGA"
BASE COUNT         3966 a         3191 c         2430 g         3704 t
ORIGIN      
        1 attttctttc aaaccaaata attaaaaata ctttcaattg tcttgaatgt atcattataa
       61 atcaatccaa gtatcggtat tcaattgtat ttttcactgt atttatcttt tttattttta
      121 atttaaatgc tgaaggcaat gactaatcca atgtctcgtc aatttttcct gcacgctatt
      181 ctgttttaaa cgtgaagcca atcctccaaa atcaaccgaa tcaagttcct gatcttgatt
      241 aaaacaagaa aaaacataag ttgtttcgcc ggttttaggg tctttatgcg gttgcaagca
      301 ttgagcacag atttctttca tcatgcattg catgggcgaa ttaattgaac caattgcaaa
      361 gtgactatcc ttcaaataag gttttaactg ttgatgccgc gcaataccca ctgcggccat
      421 catccgatca gacccaattg caatgattcg gtccgcatct ttcagcgata tagactgaga
      481 acctaaagca ccgctaccat aagcaaccat cgcctgtaca atattaccaa caaaactaaa
      541 atcaccttgc cgagaaggtt caaatccggg ctgttcatca caacaccata caatggtgtc
      601 tgctgctgct tcaatttcag ctactttgta acgatcgact agctttttat aacctgcaaa
      661 gtataaaacc ttacagcctg cagcacgcgc tgctgctcca atcgaaaata agactgcatt
      721 acccagccct cccccgacta aaactacggt ttcattctgg aatatctcag taggtgtgcc
      781 agtagggccc atcaaaatta ccggatcacc aggatttaat aacgtgcata aatcagccga
      841 tccgcccatc tccaatgcga ttaatgacac aagtcctttc gaattatcaa cagaggcccc
      901 tgtcaaggca atcccttcca tcgctagacg cgtatttact gcaatcttag ataacgttgc
      961 aaaattttgg aaacgataaa attgacctgg atgaaaatgt ttcgcagcta atggcgcatg
     1021 aacgattact tcgacgatat taggcgtcaa ccgttctacc ttatatactg ctgccctgag
     1081 ttggcgattc aaattatcga taaattcatg atctacttgg ggattcactg gcttaacttg
     1141 acttaaaacc ccactcacta ccgagtaacc ttgcttagca ctcgccatcg ctttcacaac
     1201 gttgccagaa tacgatggat gaagatcgcc aaaaaaacta ataaaacgcc catctgagca
     1261 acggcttaac agaactgctg gcgtttctgg tttagggtta cccttggtga tactaactgg
     1321 attacctctt tcatcgcatg cagcgaaata acgtccgtcg agcttaaaat atttttcatc
     1381 ttctcgagct aatacagtat taggctgtgt tcctgctgca acaagaatag cacgagcagg
     1441 caattcaact tccttggatt gatgccattt gccattttca tccatggact gaacagaaaa
     1501 tcgaattgac cgcgtatgtt cccattgatc taattcgatt cgtaccggtg tcagaccctc
     1561 agcaaaccat atcccttcct ccaatgcttt ctcaacttct tcatggttta aggtataaga
     1621 aggactatca acgagtcttt tacgataagc gagtgttgcg ccaccccaag cttgtaataa
     1681 ctttataata ttagcatcgc gcccttcttt ctgcgcctga agtcgctctg ctcgaattgc
     1741 acgcgcatgc atcaaaaatt ccgttgcaat ttctcgttct tcttcatccc aactccgttg
     1801 gatagaatct tccccttgaa ctgcagcaag agtttcatag cgctgcaaaa atttctctac
     1861 ttgaataggg taatatgcaa gcgactcagt cgcagtatca atagccgtta atcccccgcc
     1921 aatcactaca acaggcaaac gtaactgcat attagcaatc gagtctgttt gtgccgcccc
     1981 tgttaattgc aatgccatta aaaaatccga tgcggctctc acaccccttg ccaatccatt
     2041 gggtaaatcc agtactgtcg gacgacctgc ccctgctgcc aatgcaatat ggtcaaaacc
     2101 cagattcagc gcatcatccg ccgtcagcgt tccaccaaag cgtaccccgc caaaaagcgc
     2161 aaactcgtta cgacgttcta ataacaatcg aataaactgc aagaaatttt tatcccatcg
     2221 aacggtaata ccatactcgg caacaccgcc aaaaccaccc ggcatgcgtt cattcagatc
     2281 aacccgtagc gagttactat catagatcgc agaaaatgga acacgcgtac cattgagatc
     2341 aatgccactc atttctttag gtaatggctc aatctttaat ccatcgattc ccactaccgt
     2401 atggccatca ttcatcaaat ggtgtgcaag cgtgtagcca gccggtccca taccaacaac
     2461 tagtactttt ttacccgttg ctggtttagg taaaggtctt cgcaagtcga gtggattcca
     2521 gcgcgttaac aaactataaa tctcgaatcc ccatggtaac tccagcacat ctttgagtgt
     2581 tcgggtttct gcttgaggaa tattaaccgg gacttgcttt tgataaatac atgatttcat
     2641 gcaatcgtta cagatacgat gcccagttcc ggcacacatc ggattatcga gcacaatcat
     2701 cgctagacta ccaattgcgt ggccttgtgt tttaagctta tgaaactctg aaattctttc
     2761 ttccaacggg caaccagcta ataaaacgcc caactcactt tttttgaatg aaggtaattc
     2821 ttccgagctt ttaggcttct ggataaatcc tttagagcaa gaatccttac cttgttcatg
     2881 acaccagatg caataattcg cttcatccag tgcgcctacc aaatcagtcc ctgtatctgt
     2941 cagtgaaaac ccttctcggt gacgtaaatg gtctaaacga tgcagcggaa agcccttttc
     3001 attgtccgtc gagagcgata ataaatgtaa aaaatctaat tttgcgggtg ttttaaataa
     3061 aatacccgct tgagtatgca tttgaccctc aggagttctt aaagcccaag ctgcataatg
     3121 gagggccttt tctattcttg ccgcattgtt cgcttcatca tccaaccagt tagctacatg
     3181 agtagcaaat accagctctg agaaaggttc gccaaattca acttctaagg ctttctcaag
     3241 cgctattcca tcaatcgtcg ataatatctc atcacttact ttagttttag cgcgccgctg
     3301 cacaaactgt ctcttacaaa aatataaagg cgctagctca tgatgctttg ctgctaaagc
     3361 acgaacttct ttttcgattc caaataattg accgataaaa tcctctgccc agggagcgag
     3421 atcaattaac aatatcgatt catcctttgg cgcaatttca tttggactca aacgtgcttg
     3481 atctaaacga gccctaagta ccggatcagc acttccaaga aattctaaaa atgcctgatc
     3541 taatttcagc agaccatcgc gacgataaag ctctaagaaa ctgaccccaa atttaagctc
     3601 gggagccata actgtcgatg aaaattgtgt agatgttgat tgtgaataag tcattttggt
     3661 taatttttat tgtaaaaatc ctactaaaat tttgctttgg ttaaactcaa tacaaatctt
     3721 tcagaagatc tatgcaaatt gccagcgctc tagcaagctt gaatcaaagc aagggagatt
     3781 acaaaatagg attggacttt agcagatcat tccagctgca accgtattat tagttgtttc
     3841 atcaattaaa ataaaactac ctgtggcacg attgcgactg tatgcatcaa aaacaagagg
     3901 ttgttgaact ttcatcacaa cacgcgcaat atcgttcatc cttaaggagt ctgttttctc
     3961 ttgattaagc gtattcacat caacacgata atcaatttga ctcacgagcc cctttaccat
     4021 acgcgtagta tgcttaacta tatatttacg tccggaactc aaaggttgtt ccgacaacca
     4081 acacaacatc gctgtaaatt ctttatcgac tcgtggttga tcatctgatt ttattagcat
     4141 atccccacga gagatatcca aatgatcctc aattgtcact gtaacagact ggggcgcaaa
     4201 ggcttcgtca aaattgcttt gataaagcaa aatttcttta attcgagaat gtaatccaga
     4261 gggctgaatc gttaccgcat caccaacctt aattgaacct gattcaactc gacccatgta
     4321 acccctgaaa tcatgaagct cttcggtttg aggccgacaa acccattgaa ccgggaaacg
     4381 aaagtcatcc aaattaacat cgtgctcaat atcaacattc tccaacaaat cgattagcgt
     4441 tcttccttga taccagtcta gttgatcacc gcgttctaca accatatcgc catttaaggc
     4501 tgacatcgga atatattcaa tgttacgcaa attaagatgc tgcgaaaaca ctgcaaaatc
     4561 atcgcaaatt tgagtaaata cttcttgtga atattccact aaatccatct tatttactgc
     4621 cacaacaata tgaggaatac cgacaaggct cgcaaggtaa gtatgacgtc gagattgagt
     4681 caagaccccc ttacgtgcgt cgataagaat gatcgccaaa tttgccgtgg aggcacctgt
     4741 taccatattt cgggtatatt gctcgtgacc gggtgtatcc gcgataataa atttacgttt
     4801 aggtgttgcg aaataacgat acgccacatc gatcgtaatg ccctgctcgc gttctgcttg
     4861 caaaccatcc gtcaataacg acaaatcaac attctgcata ccacgtttgc tggatgtttt
     4921 tgcaatagag cttagctgat cttcaaatat tgattttgaa tcatgtaata atcgaccaat
     4981 tagcgtactt ttcccatcat ctacactacc tgccgcaata aagcggagta gttcagcacg
     5041 atcaagccct acattttcaa ctaccgacat ttgataacct tctatattaa acttttagat
     5101 tgatttattt tctgtatcag attcacaaaa ctaaaaatag ccttcttttt ttcgcaattc
     5161 catcgaagca tctgaagttt gatcatccat gcgagtcgca cctctttcag taatgcgagt
     5221 cattgccgtt tcagcaataa tttcaccgac actagctgca tccgatgcaa ccgggcaagt
     5281 acagctcata tcccctactg tgcgaaaccg aaccatgatt ttctcaattt tttcattatc
     5341 tctcggttgt acaagatgcg aaacaggcaa taggcctgaa tcacgaggaa taacctcacg
     5401 ccaatgtgca aaataaatag atggaacttc gagattctcg cgagctatat attcccacac
     5461 atcgagctca gtccagttac taattggaaa cactcggata ttttcaccag aatgtgtttt
     5521 ggtattgtac aaactccata attcaggacg ctgattcttc ggatcccatt gaccaaactc
     5581 atcccggaaa gaaaagatgc gctcttttgc gcgtgctttt tcttcatcac gtctagctcc
     5641 accaatgcaa gcatcaaagc taaactcagc aatcgcatct agcaatgtaa ttgactgaaa
     5701 tggattccta ctctgatttt ctgaccgcag taccactcga ccttttttaa tcgagtcatc
     5761 catacttcta acgatcaatc tctctcctag ctctttagcc cgatgatccc gaaatgcaat
     5821 tacttcaggg aaattgtgct ctgtatcgat gtgcaagagc ggaaacggga atcgacctgg
     5881 acgaaacgct ttctctgcga gacgaagcaa tacaatagaa tcttttcctc ctgaaaaaag
     5941 taaagcagga ttggcgcact cagcagccac ctcccgcatg atgtgtatag cttcactttc
     6001 cagggcatcc agatgagtaa aagatcgatg gttcattttt ttactaaatc aatataaatt
     6061 aattatctac tcaagcactt actgctcgac ttacgtgtaa tccacattct tttgagtcag
     6121 gattctccca ccaccagcgc ccagatcgaa tatcttcccc cggcgtgata gcccgagtac
     6181 aaggcgcaca accaatacta ggatagaaat gatcatgcag cttgttatag ggcacatcat
     6241 gttgcttgag atagctccaa acttcagcat tactccagtc acataatgga ttaaacttct
     6301 gaatcgcatg ttcagcatcg aactgagata ttgataaact atctcgtgta atagcttgct
     6361 cacgccgcat acccgtcacc caagcgcgtt tgcctgttag agctcgtcgc aagggctcaa
     6421 ccttgcggat atagcaacaa cgtttacgta attcaacact ttgataaaac ccattgggac
     6481 cattctgcgc aatataactt tccaccgcag ccgcttcggg aaaatacgcc ttaatattga
     6541 tatgatattg atccctaacc gtctgaatca gtgcataagt ttcttcaggg agtcgacccg
     6601 tatccagcgt aaatatctca atattgggaa aatagcgtgc aatgagatct gttaaaagca
     6661 tatcctcagc accgaagcta tttgcaaata cagccggcat aaagttttca tgtatatcct
     6721 cgagtagccg ggatacttct tcaactttat gctcaatatt aatttcagtc attccattaa
     6781 ttagcgttta tctacgaaac ttatagtgca gtagttgact gctgagcatg cttcattggt
     6841 atcgatgaca cagctttagg caacgcccct gccagatcaa gctggcgatc aaaatcgata
     6901 caaaaatcag ccacttcagc agtccactcg tcaagcgcag cataccattc ggtcaaggcg
     6961 ctatcggtta gctcttctgc tggagttgag aaagcttgtg cgaatttagc tagcccaact
     7021 gcaagattag gtttcgtcgg caaaacacgc ttgagctcct cggcttgctc tgacaaattt
     7081 tcaaatttct taccacccac gagctgcgcc aaaccttgac tgatcagtct tgcgatctct
     7141 tcagcacatt ttaaggaatc ctctaattta cgctgaaact taagtgcatt acgataattt
     7201 cgttcattcg ccgcatcaaa aaaagcagta ccaatttgga gttgtttggc gccggcacac
     7261 tcacccccac ccaattgcaa caccctaaaa tcatcccctt gaccatggtc tcttaaaaca
     7321 gcgtgtaaat cttcttgctt aacttcaatc acatccgcca gcagctcagt aaaataacct
     7381 tgcccaactc gcctggtcca attaaaaaac gactcaccat tttggcgatt tgttgcataa
     7441 gctgattgaa cgcgatcaac cgcaacttca acacgcgcac ttggtaccga aggcccttta
     7501 aatgccaacc ccccttcagc cataccattg ccaccaaaat acatctgata atggggaaca
     7561 agtttgccat acatccgctt tccttcccca taaatcccaa tatcaccact ttcaggttgt
     7621 gcgcaaccat tatggcaacc agaaactcta atacgcagat catctttacc gcctgtcaac
     7681 tttgctccaa cagtaggact agaagtgatg cccaaccgac acgtcgacgt tcctgggcaa
     7741 gcaacaacat catcccccgc cttgggcaaa ctcaacccta actcgtttaa cgtggcttgg
     7801 atatctgtaa ttttagcttc cggcacatca accaacatta aattctgatc ctgagttgtt
     7861 cggatttctt tcaaatcatg ttgcctaagc aactttgcga tcccacgcac ctgcttggcc
     7921 gtaacctgac ccagcgggac actaataggt accacaaata accccgcttg tttctgggca
     7981 aacaatttac gtggtgcccc tgggcctggc acttccgatg ctcgattacc taaagcccaa
     8041 tcacctttag ggtagtcctg atcagccaac acagctttaa cccgcgctaa ttcctcacgg
     8101 tatttctcga caaacccatc gactccaaat ttatccacta agaatttaat ccgagcctta
     8161 gctcttttat tgcggtcaga atacttgttg tgcatggtca acacagcttc gacaatgagc
     8221 agcagatctt tttcttcagc aaacgattca attacgatag cctcgtgtgg cttatgtccc
     8281 aaccccccgc cgactaaaac cttgaaacca aaccgatcgc cttgttgtac tgcaaccact
     8341 cctaaatcat gaatcatcgc ctgcgcacag tcagcctcac aacctgaaaa gctcatctta
     8401 aatttgcgcg gcattaattg gttaagcggt ttacgtaaaa aatgtcggac cacgccgccc
     8461 aaatgctgac tgatatcggt atgttctcgt gaacagactc cagttagcgg acatgcagtt
     8521 acattccgaa tagtattacc gcacgcttct ctcgttgtaa gcccaccttt ggctagatgc
     8581 cttataactg caggtgtatc ggctagcggc acataatgta gttgaatatc ttgccgcgtc
     8641 gtaacatgcg cttcaccaga ttgaacatgc ttctcgataa catcagcgat ggcatccaat
     8701 tgtgatatat tcaatcgtcc acccggtact ttaatacgca ccatattaac cccatcttgg
     8761 cgctgtccat atattcccat ctgtaaccgg gtagcagtaa atcgatcaac atccattttc
     8821 tgcgcaaaga aatcctgcaa tgattgattg taggagtcaa tttcctcatc attagccaga
     8881 tcagttaaat aatccatcta tttcttctct ccatttttca ctcatatcaa cttgctactg
     8941 atatgtaagt aaggtattaa taatttacgc ctattttatc tgatatctga tcgctggcgg
     9001 taatttctcg atcacttccc ttctattttt ttatgagtcc caattcgagt ctgtcgcata
     9061 ttgggctatt gatcgatcaa atagatacga ttgcagttta ttcagggcaa aaaagtgagc
     9121 tatggtatca gcacttacat atacctcaaa ataatattat attacaataa tataattatt
     9181 aattatataa gaacacgatg agactgcaac aattacgtct tttatgtgaa atcaatcgcc
     9241 aaaatttaaa tctttccaaa gcagcagaaa gcttacacac ctctcagccc ggtattagta
     9301 agcaaattca gttactcgaa gaagagctcg gcgtagatat ttttgtacgc aatggtaagc
     9361 gtatcgttaa aatcaccccc cctgggaagg cgattatcga aattgccgaa cgtatgctac
     9421 aagatgctga aaacttgaaa tcggttggcc aagagtttaa caatcaaacc agcggcacac
     9481 tcactatcgc aaccactcat actcaagcca gatatgcgct accaccggtc attaaacgct
     9541 ttacggagcg ctaccccaat gtaaaactta ccctgcgaca aggaaaccct acccaaatcg
     9601 caacactagt ctcatctggt gaagccgatc tagccatcgc caccgaagca atcgagctat
     9661 ttcatgattt aatcatgcta ccgtgttatc aatggaatcg ctgtattgtc gtaccaccca
     9721 accatccact cttagatagc ccaatcctca cacttgaagc catcgcacaa tatcctattg
     9781 ttacctatga tttcgcattc acaggccgtt caaaaattaa tcgggccttc gaagcaaacg
     9841 gattaaatcc aaatgtagtt ttgactgcta tcgattccga cgtaatcaaa acttatgttg
     9901 aattaggatt aggcattggg attttagcga acatggcctt tgatccttta cgcgaccaac
     9961 accttcgatc aattgatgct agtcacttgt tcgaacccag cacgactcga gtcggtatta
    10021 gccgcaacag ctatattcga gggtatagct atgatttcat agaaatgttt gccccgcacc
    10081 taactcatga tgtggttaaa tcggcaatga agggcaagcc taacaaaact gagccttttt
    10141 cattctgagc gcagaaaaac ttcattcccc ccaatataca atagcagacc ctccatttct
    10201 gaatacctag ctttgctgtt ttttcaatac aaacaaaagc tatgccattg atctcccagc
    10261 ttatcaaaaa aaataaccct attgtcaacg gaaccctatt tgatcgcgtt attggtacct
    10321 ataaagctac aaaagcaaaa taaaagttaa tcacatgaga taactataaa tcacgaggga
    10381 aaaatgcttt taaccaatca atataccgcg aatgccagca aagccatcgc acccattcca
    10441 ataaatctag cctgttttgt tattcgctaa cattctctag tttcattttt acgattttaa
    10501 ttaagtttcc actacagtta tggaaaagct caatattgct tatacatgca acgtcagaaa
    10561 accgggcatg gaagatgaac gatacggtga attcgaaagt cccgagacga tcgaagctgt
    10621 tattagtgcg cttgaaggag caggggcgaa tgtagaatta atcgatgtag ggccagacat
    10681 ttttcagcag cttgagaaac gcaaaaacag cattgatatc gttttcaata atactgaagg
    10741 gttgaatgaa aaggagttac gggaagctat cgttcctttc ttttgtgaat tcttaagcat
    10801 tccttatacc gggagcagcc ctaaaacctt catcaacaaa atggacaaag caacagccaa
    10861 acggattgta gcttacgatg gagtaccaac agcgcgcttc caactgatga aacctcagga
    10921 tcgacttaga gatctagcat ttcctctaat ggtcaaacct tatagtgaag ggaccagtat
    10981 cggtattaac caaaaaagca aagtgaataa tttagccgag cttgagcaag caatagaaac
    11041 catttttact caattcaacc aaccagctct cgtggaagaa ttcctagatg gaacagagta
    11101 cacgattggt tttattggaa attatctatt acccatccta gaaatagact ttgcaaagat
    11161 tccaggacag ccacaactca gggatcctta cgtaaaaagc atcgagaatc ctttcatttc
    11221 gcacttaccc tggaacgaaa aagctagaag ttttgcgtca ctttcaatta aagcacatga
    11281 ggcactggaa gttcgggatt ataaccgtat ggattttcgt gaacggaatg gtcagcttta
    11341 ttttctggaa gccaatgtca ttcctgggct acaccctacc gaagctgatc taactaacat
    11401 gtgccgtcat gccggtgttg ctcacgcaga catggtagta ctaatcctaa gtgctgctgt
    11461 aaaaagacta tctgagcagt atccggaacg atttaagaaa aaaacagaat tactcactga
    11521 gttagccaat aaagcgatct gcaaggcgac tcatgctggt gaaattgttt atcaagaccg
    11581 agtatacaac cttctgggat aattatttta ggactctaca caatgatcgt acctatctca
    11641 caagaaatgc atccccagct tatcaaaatc gctttgcaag aaatgggggc tatgtatcaa
    11701 cctgagcttc atgaagagct agatgatatc gaaaggggga gatgtttcgg gctttgtaag
    11761 ctagaaaacg gtaaacccgt cggatacgtc gtggctaaaa gaacaatcga tgcttaccac
    11821 ctccttacca ttgctgtcga tatcgagcat cgcggtaaag ggtatggctc cttattgatc
    11881 gacagtattt ttgctgaaat cgaaaaacag ggggggcgga tccttaatgt gattactgac
    11941 gcagaagcga acgaaagcat acgcttctac ctcaagaata gtttcgtctt ggcaggcatc
    12001 gtacaagacg aatttatttc tggcgtggct caagttcatc ttacaaagcg tgcaggctac
    12061 agcccagaac aatattgaaa attcttttta cttataacaa tcaaataaaa taactattct
    12121 aggggcacaa aacatgagaa ttagagcaac aaaaatggat ggatgtggta actctttcct
    12181 agttatcgat gaaataaaaa atccattggc tcggttcgat cgaaccaaaa tttcaaatgc
    12241 acttgcagtt cagcataaca cagatggaat actctatctc gatcagcaac atgggatgcc
    12301 aaccatgaaa atttttgatc gagatgggac agaagaaacc atgtgtggta atggactacg
    12361 ctgcgctact cgatatttcc gcgatcaata catttcaagc aatatttttc caatcgttac
    12421 cggcgatggt attaaagaag tcacggttga taacgagctt gttgaagtca atctaggatc
    12481 agcacgtgat tattgccaag taagcgataa aatgcatttt gtgtttaccg gtgttccaca
    12541 cctcgttgtt ataggtaacg agatgagcct tgaatctgcc aaaacactag ggaaattgct
    12601 gagggaagat aaatctcttt gcgcacgagt caatcatccc gaaggtatca acgtcaattt
    12661 tatctggaga acaattgaag gaatcgctgt acgcacctat gaagtcggtg tggaagacat
    12721 taccctcagt tgcggaacgg gaagcgcagc cagtgcatat gtagctgcaa aagtttatga
    12781 tttatccctg cctattacga cgcaatcgct tgggggtaac ttagaaatta gcgatatcgg
    12841 cggagaatta tcgattcgtg gcataactcg ctatcaagga aaagtcattg gattcgtaaa
    12901 tcacgctcac cccaaatcga ctattccttc tcgatactgg caacgaagca atgtttcatt
    12961 aagcctataa atccaataat taatttaaaa ccaatatgga attttctatt gttacctttt
    13021 tactgatcat ggttgcaatg gcttttgcag acgtttgctg gacactctac tttatcgatg
    13081 tagaagaaag gaaagcgcac ccggctgcat tctggtcagc catgatcatt ctcgttacag
    13141 ccttttctgt gacaaattac gtcgaaaaca aaatttatat tgctgccgct tttataggtg
    13201 cctacatcgg tacttatgga accctcaagt ggaaattaca caaagaaaaa catcatgcgc
    13261 aatccgaatc atcccagact aaaggagcat a
//
LOCUS       DAOQLJ010000053        13223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1755_length_13223_cov_6.151428, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000053 DAOQLJ010000000
VERSION     DAOQLJ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 13223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13223
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1755_length_13223_cov_6.151428"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PK522_05350"
     CDS             complement(<1..819)
                     /locus_tag="PK522_05350"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNC41192.1"
                     /translation="MKLLIKNGRLIDPKNKIDAVKDLCIAEGKIVAIGSVPTEFTDCP
                     VIDAKDWVVCPGLIDLSARLREPGLEYKATLESELDAAAAGGITSLACPPDTDPPLDE
                     PGLVEMLKHRTKSLNHIHVYPIGALTQKLKGEKLTEMAELRDAGCVAFSHADEPITNL
                     HILRQAMYYASTFNFSVWLRPQEINLANRGVAHDGEVATRLGLSPIPVCAETIALSNI
                     ILLVREIGVRVHVCRISSAESVAMLRTAKQQKLPITCDVSINHLHLSEMDIGFFN"
     gene            complement(900..1850)
                     /locus_tag="PK522_05355"
     CDS             complement(900..1850)
                     /locus_tag="PK522_05355"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNC41193.1"
                     /translation="MNHQLQLNKNGELLHLLTIEGLPASILTLILDTAESFTGVTERE
                     IKKIPLLRGKSIFNLFFEPSTRTRTTFEIAAKRLSADVLNLNIAVSAQSKGEALLDTV
                     NNLSAMHADMFVVRHSQSGAAHMIASHVKPDIHVINAGDGRHAHPTQALLDMFTIRRY
                     KQDFHNLRVAIVGDILHSRVARSQIHALSTLGVPEIRVVAPKTLLPNKVERLGVHVYH
                     DMEKGLRDVDVVIMLRLQNERMQGANLPSNEEYFKYYGLTPEKLAKAKPDAIVMHPGP
                     MNRGIEIDSVVADGGQSVILPQVTFGIAVRMAVMSILAGN"
     gene            complement(1847..2362)
                     /gene="pyrR"
                     /locus_tag="PK522_05360"
     CDS             complement(1847..2362)
                     /gene="pyrR"
                     /locus_tag="PK522_05360"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNC41194.1"
                     /translation="MSQRLPDAEFLLTELIKQLTPIAHESMAMVGIHTGGVWVAQRIH
                     QALNLKLPLGVLDISFYRDDYSKIGLHPQVKPSALPFEVENSHIILVDDVLYTGRTIR
                     AAVNEIFDYGRPASIHLAVLVDRGQRELPIAAQYVGAVLSLPTNNMLELKQQSDGRLS
                     LGLLDVKLAAE"
     gene            complement(2343..2753)
                     /gene="ruvX"
                     /locus_tag="PK522_05365"
     CDS             complement(2343..2753)
                     /gene="ruvX"
                     /locus_tag="PK522_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNC41195.1"
                     /translation="MAFDFGKRHIGIAIGESELKLAHPLTTITSEVIKIQFEAIAKLI
                     TAWEPKLLVVGLPLHDDGAEHEMTRLSRLFARRLEGRFRIKVVMVDERFTTRSARLTL
                     NELGIRRSQQDRFIDQVAAQHILQSFFDHVPTTP"
     gene            complement(2855..3415)
                     /locus_tag="PK522_05370"
     CDS             complement(2855..3415)
                     /locus_tag="PK522_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNC41196.1"
                     /translation="MENVNLTDHFLIAMPNMADPFFAKTLTYICEHNERGALGLVINR
                     PTDLSLEKFFDQLGIPRIDTFPKEPTVLFGGPVQIDSGFVLHKPIGNWKSTLAVNPKV
                     GLTSSVDIFHAIANCEGPDEMLVTLGYSGWAPGQIEQELAQNAWLTVPASHSIIFEMT
                     FDERLPAAIKLLGINFSNLSNQIGHA"
     gene            complement(3468..4094)
                     /gene="coq7"
                     /locus_tag="PK522_05375"
     CDS             complement(3468..4094)
                     /gene="coq7"
                     /locus_tag="PK522_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HNC41197.1"
                     /translation="MFNIDRVIIGLDNALRTLAAPAQTYRPVPGDSLPDHELTEVEKK
                     KSIALMRVNHVGEICAQALYQGQALTARNQVVQKTLTQAAREETEHLAWTERRIAELG
                     GRKSLLNPVWYGGSFAIGLVAGMLGDKWNLGFLAETEHQVEKHLAGHLQQLPTQDEKS
                     RAIVTQMKTDEASHATMAVSHGGAELPLPVKLAMKASSKVMTQTAYWI"
     gene            complement(4130..5401)
                     /locus_tag="PK522_05380"
     CDS             complement(4130..5401)
                     /locus_tag="PK522_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNC41198.1"
                     /translation="METTRLEKPQASVASWNQTEILAEADKCVACGLCLPHCPTYRLT
                     LSEADSPRGRIAMISGVMKQHIPMNERFTLHIDRCLTCRACEYVCPSKVSYGKLVDQA
                     RVKILNASVKKADVPYKIRSWLERQWIDKPWRFDTLRPFVKLFQQLGLQRLLLRLPFL
                     NGTSFRLLLSKLPPIRYPFQHWQSFYPAVGKQRGEVGLFLGCIARLIDVETHLSTIAV
                     LNQLGYSVHVPQQQTCCGALYQHRGESTHSGNLLAQNQQTFKDLNLTALITTASGCGA
                     HLVENNGFSIPVMDINQFLAEQDFSQIKFNPLAKRVAVHHPCTLRHVLRGSQFPSKIL
                     AYIPEIELKELANQDQCCGAAGTYFLDQPGIAEALLTHKIQDVLAINADYLATSNIGC
                     AFHIASQLNEQQAGIEVLHPVTLLARQMSIQ"
     gene            5796..6509
                     /locus_tag="PK522_05385"
     CDS             5796..6509
                     /locus_tag="PK522_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41199.1"
                     /translation="MNAAERIAEKYLQHMGIELVEFEPDGNVTPDFLVNKKIAVEVRR
                     FNQNYEDTTGNTLGLEETAIPLAQKFKQLLKSLGPSTNGETWFVTIDFSRPIEPWLIL
                     RPKIEVALNGLRLDASFKQRIIQVNDLVELYLIRASKDHGSAFVFGGYSDGDSGGLVM
                     AEVERNLRICIAEKEKKVAPHRHKYPEWWLLLVDYIDFGMEEDDRVVFKESVMPRIKH
                     SFDKVIFVNPLDHRYGFEV"
     gene            complement(6852..7697)
                     /gene="serB"
                     /locus_tag="PK522_05390"
     CDS             complement(6852..7697)
                     /gene="serB"
                     /locus_tag="PK522_05390"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNC41200.1"
                     /translation="MNLIIQGLDIQNHDLRHIAKLAQASHIERITGEAFRLVNASEHS
                     EIPDYCADVQLDFAYVVPNKKLSEFGLVVMDMDSTLIAIESIDEIAALCDVKPQVAEI
                     TASTMQGEIDFAESLIRRTALLKALPENLLQQVYDEKVKLSLGADKMLQKMKLAGLKT
                     MVISGGFTFFTDRIKAKLGLDFSFANQLEIADGKLTGKVVGEIIGAKGKADILKEIRD
                     HLGLSKEQVIAIGDGANDLPMLTEAGTSVAFHGKPILQGKTTFSINHVGLDAVINFFE
                     QASPY"
     gene            complement(7820..9298)
                     /locus_tag="PK522_05395"
     CDS             complement(7820..9298)
                     /locus_tag="PK522_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC41201.1"
                     /translation="MKENLIQQLRFCSTLPSLPAVALKIIDLANSADTDMIQISHYIA
                     LDPALAAKILKTANSPLYKSRRTSSNIRQAVCVLGTHAVISIALSFSLTKTLMQHTES
                     SIGKESSDLFWRRSIIAALACKALGNKINLKMADDLFLAGLLQDIGILAFFSIIPEAY
                     AKIYSSAASHDALLQAEYEAFETGHDELGYTLLKRWNLPSYVAIACMTSHNQSSSQKG
                     EPTIADCVAASGYIADYFLNPSDTEKIAQTMTKLYARLNLDGQALLKVIAKIKDELRT
                     VEELFEFSICSESELNALMSEAQELLSIQSLIKIRDLEDKTQRDGLTGAHNRAFFDSV
                     FEREFIFSTQHSTPLTIAMIDVDHFKQVNDSHGHVVGDALLIALVRAIFGQIRQDDLL
                     CRYGGEEFVLILPGTPIASARKLLIRLKNSIEAISYKLDNDKHIGITVSMGIATNLDK
                     GSIFTSHDGMLEAADLALYAAKHAGRNKIVEWDHSLSLQNHS"
     gene            9534..11039
                     /locus_tag="PK522_05400"
     CDS             9534..11039
                     /locus_tag="PK522_05400"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNC41202.1"
                     /translation="MDFAIKVGSADKQRSACIVVGVFESRKLTNAAKHIDVATDGYVS
                     NVLRTGDMNGKLGTTLLLHNIPTSLSKRILLVGLGQEKECNGKAFRTAILSAMSALLR
                     TAVSEVGLFLLDVSLKDRDLSWKISQTAMLASECVYRFDALKSKSEGEPPSLNKVTFY
                     LGDRAYLKIAEIALAQGKAIAQGMKVAKDLGNLAPNICTPSYLAKCAVDMGKSHKLKV
                     SVLGQKEMEKLKMGALLAVAKGSHQPAKLIALEYRGLAKTEKPIVLVGKGITFDTGGI
                     SLKPAAEMDEMKYDMSGAASVLGTLAAVAEMQLPLNVVGIIPTTENMPGGNATKPGDV
                     VTSMSGQTIEILNTDAEGRLILCDALTYAERYNPEAVIDIATLTGACVIALGHVASGL
                     MSNNDQLADELLQAAELSVDRAWRLPLWDDYQDLLRSNFADMANIGGRSAGTISAACF
                     LARFAEKYQWAHLDIAGTAWKSGKEKGSTGRPVPLLTQFLISRAMRKVAHN"
     gene            11133..11561
                     /locus_tag="PK522_05405"
     CDS             11133..11561
                     /locus_tag="PK522_05405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HNC41203.1"
                     /translation="MVQVDFYTGAADKLSTACRLCIKAVQQMQRMLILSDDAPQLTQL
                     DKLLWTFSSVSFIPHCFVDNQLSAITPIVLGSPAHVYPEALNYGILLNFHDEILPYYH
                     CFDRVIEIVDHTAADKEKARKRYRIYQAQGCQVRHHNLDG"
     gene            11588..11989
                     /locus_tag="PK522_05410"
     CDS             11588..11989
                     /locus_tag="PK522_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41204.1"
                     /translation="MTDHLDVPPEPEDNTLLNKLDAILKKYRKEYSAVIRPADLKATP
                     AIPEPPTPIVTPAPTQNQSDVLPSFTVPEIPILTERIVVAPEISFNESGISLLLCYAF
                     DSALKDAKLDLTSTQRFELLQALAQRLPKNL"
     gene            12055..>13223
                     /locus_tag="PK522_05415"
     CDS             12055..>13223
                     /locus_tag="PK522_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNC41205.1"
                     /translation="MELAKSFDPSEIEARWYPEWESAGYFQPLNEGGVTPYCIMLPPP
                     NVTGTLHMGHAFQHTLMDALTRYHRMLGQKVLWQPGTDHAGIATQIVVERQLDQQNLD
                     RRSLGREKFLERVWKWKEESGSTITRQMRRMGTSCDWSRERFTMDETLSKAVTEVFVQ
                     LYREGLIYRGKRLVNWDPVLQTAVSDLEVISAEEEGSLWLIRYPFEQQDGNAALTDGL
                     IVATTRPETMLGDMAVAVNPDDDRYSHLIGKHLRLPLSERTIPIIGDSYVDPTFGTGC
                     VKITPAHDFNDYQMGLRHKLVPLNIFTLDGKINEFAPSEYQGLDRFDARKRILADLKA
                     HGLLVETRPHKLMIPRGDRTQSVIEPMLTDQWYVAMEDMAKRGLDVVAQGEVKFVP"
BASE COUNT         3631 a         2780 c         3053 g         3759 t
ORIGIN      
        1 attaaagaaa ccaatatcca tttcagaaag atgcagatga ttgatagaga catcgcatgt
       61 aatcggtaat ttttgttgct ttgcagtgcg aagcattgcc acgctttcag cgcttgagat
      121 acggcacaca tgaacacgaa caccaatttc acggaccaat aaaataatat tagataaggc
      181 aatggtttca gcacaaactg gaatagggga taacccgagg cgtgtggcga cttcaccatc
      241 atgtgcaacc cctctattcg ctaggttaat ttcttgaggg cggagccaaa cactaaagtt
      301 gaaggttgat gcgtaataca ttgcctggcg caaaatatgg aggtttgtta ttggctcatc
      361 tgcgtgactg aatgcaacac accctgcatc ccttagctcg gccatttctg tgagtttctc
      421 gccttttaat ttttgggtta gcgccccgat aggataaaca tggatatgat tcaagctttt
      481 agtgcgatgt tttaacattt caaccaaacc cggttcatcc agtggagggt cggtatcagg
      541 ggggcaggca aggctagtga tacctccagc tgcagcagca tcgagctcgg attccaaggt
      601 tgctttatat tcaagccctg gctctcgtaa acgcgcagac aaatcaatta gcccaggaca
      661 aaccacccaa tccttggcgt cgataacggg gcaatccgta aactctgtgg gaacgctacc
      721 aattgcaacg attttgccct cagcgataca gagatcttta actgcatcga ttttattttt
      781 aggatcgatc aaacgtccat ttttgatgag caatttcata aattggatgg gtgtttactc
      841 aggtaatact attataaagg aaggttattt ctcgtcatgc taataggtat aagatcgcgc
      901 taattaccgg ctaaaatgga catgacagcc atgcgcaccg cgattccaaa cgtaacctgc
      961 ggtaaaatca ctgattgccc gccatcagca acgacagaat caatctcgat tcctcgattc
     1021 attgggccag gatgcataac aatggcatct ggctttgctt tagctaattt ctccggcgtg
     1081 agtccgtaat atttgaaata ttcttcattg ctcggtaagt ttgcgccttg catgcgttca
     1141 ttttgcaagc gcagcatgat gaccacgtcg acatcgcgca agcctttttc catatcatgg
     1201 taaacatgaa cgcccagccg ctcgactttg ttgggtagta atgttttagg agcaacgact
     1261 cgaatttccg gtacgccgag cgtgcttagg gcatgaatct gtgatcgagc cactctcgag
     1321 tgcagaatat cgccaacgat cgcaacgcgc agattatgaa aatcctgctt gtagcgacga
     1381 atcgtaaaca tatcgagtaa cgcttgagta ggatgagcat gtcggccatc acccgcgttg
     1441 atgacatgaa tatcgggttt tacatgactt gcaatcatat gcgctgcgcc actttgcgaa
     1501 tggcgaacga caaacatgtc tgcatgcatt gcagacaggt tgttgacagt gtctaacaat
     1561 gcttcccctt ttgattgcgc tgaaaccgca atgtttaggt taagcacatc tgcagataga
     1621 cgttttgcag caatttcaaa tgttgttcga gtacgtgtgc taggctcaaa aaaaagattg
     1681 aatatcgatt tacctcgtaa cagcgggatt tttttgattt ctctttccgt gacaccggta
     1741 aaggactctg ctgtatcaag aatcagagtc aaaatcgaag ctggcaagcc ctcgatagtc
     1801 agtaaatgaa gtagctcacc gtttttattg agttgaagtt gatgattcat tctgccgcca
     1861 gcttcacatc cagtaagccc aaactaagtc ttccatcgga ttgttgctta agttctaaca
     1921 tattattggt gggtaacgat aatacagccc caacatactg agcagcaatt ggaagctctc
     1981 gctggccgcg atcgactaac acagccaaat gaatactggc aggtcggcca taatcgaaaa
     2041 tttcgtttac ggccgcacgg attgttcttc cggtataaag cacatcatcg actaaaatga
     2101 tatggctatt ttcaacttca aacggaaggg cagagggttt gacctgggga tgcaggccaa
     2161 ttttactata atcatctcga taaaatgaga tatccaggac gccaaggggt aatttaaggt
     2221 tgagcgcttg gtgaattcgc tgtgcaaccc atacgcctcc cgtatgaata cccaccattg
     2281 ccatcgattc atgggcaatc ggtgttaatt gcttgatcag ctcagtgagt aaaaactctg
     2341 catcagggag tcgttgggac atgatcaaag aaagattgaa gaatatgttg tgctgcaact
     2401 tgatcgataa aacgatcttg ttgcgagcgg cggataccta attcattgag tgttaatctc
     2461 gcactgcggg tcgtaaagcg ctcatcaacc ataacgacct tgattctgaa acgaccttct
     2521 aatcgccttg caaataatct gcttaatcga gtcatttcat gctctgcgcc atcatcatgc
     2581 agcggtaatc caactactaa taattttggt tcccaagctg tgataagttt tgcgatggct
     2641 tcaaattgta ttttgattac ctcactagtg atcgtggtca gcggatgagc caatttcagc
     2701 tcgctttcac caatagcaat tccaatatgg cgttttccaa aatcgaaggc catcaccgtt
     2761 ccttggaaac aatactggga atcgttaatc gtacttaaag tgctttccga gttgctcgtt
     2821 accattgaac ttagatgatt actttgtggc tcatttaagc gtgaccgatt tggttggata
     2881 aatttgaaaa attaatacca agcaatttga tcgctgcagg caatcgttca tcaaaggtca
     2941 tttcaaaaat gatgctatga gaagcgggta ctgtaagcca ggcattctgg gctaattctt
     3001 gttctatttg acctggtgcc catcccgagt aaccgagagt gacgagcatt tcatcagggc
     3061 cctcacagtt tgcaatggcg tgaaaaatat caaccgagga agttaatcca acttttgggt
     3121 tgacagcaag tgtcgatttc cagtttccaa tcggcttatg taacacgaag ccactatcga
     3181 tttgcacggg gccaccaaat agaacagtcg gttctttagg aaaggtatca atccgaggga
     3241 tgccgagttg gtcaaaaaat ttctctaatg agagatcagt gggacgattg atgactaatc
     3301 ctaatgcccc acgctcattg tgttcgcaaa tataagtcaa tgttttggca aaaaagggat
     3361 cggccatatt cggcatggcg ataaggaaat gatctgttaa attgacattt tccattgctt
     3421 caacgctcaa aacattgttt ttactgttgc ttggatacga ttattcatta aatccaatat
     3481 gcggtttgcg tcattacttt cgagctggct ttcattgcta acttgacggg taaaggcagc
     3541 tcggcaccgc catgagaaac cgccatcgtt gcatggcttg cttcatcggt tttcatttgt
     3601 gtaacaatcg cacgactctt ttcatcctga gtaggcaatt gctgaaggtg tcctgctaga
     3661 tgcttttcca cttggtgctc ggtctcggct agaaaaccga ggttccattt gtcccctagc
     3721 ataccagcga caagaccgat tgcaaatgat cccccatacc atacgggatt gagtaaactt
     3781 ttacgtccgc ctaattcagc gattcttctt tcggtccatg caaggtgctc ggtttcttcc
     3841 cgagcggctt gagtcagtgt tttttgtacc acttgattac gtgcggttaa cgcttgccct
     3901 tgatataacg cttgggcaca gatttctccc acatgattga ctcgcatcag cgcaatcgat
     3961 ttttttttct ccacttccgt tagctcatga tctggcaagc tatccccggg aaccgggcga
     4021 taggtctgag ctggcgcggc gagcgtgcgt aatgcattat ctagtccgat aataacgcga
     4081 tcgatattga acatctcttc caagctcctt ttcaataata gattttcaat cattgtatac
     4141 tcatttgacg agcgagcaag gttactggat gcaaaacttc gattccagct tgctgttcgt
     4201 ttaactgact tgcgatatgg aatgcgcacc caatattgga tgtggcgaga taatcagcat
     4261 taattgcgag cacatcctgt attttatgag ttaacaaagc ttctgcaata cctggctgat
     4321 caaggaaata agtccccgcc gcaccgcaac attgatcctg atttgctaat tctttcaatt
     4381 caatttctgg gatgtacgcc agaatttttg agggaaattg cgatccgcgt aaaacatggc
     4441 gtaaggtaca gggatgatga accgcaacgc gtttcgctaa aggattgaat ttaatctgac
     4501 tgaaatcttg ttctgcgagg aattgattga tatccatcac gggaattgaa aagccattat
     4561 tctcgactaa gtgagcacca cagcccgatg cagtcgtgat caatgcggtc agattcaagt
     4621 ctttaaaagt ttgctgattc tgtgctagca aatttccgga atgagtggat tcaccacggt
     4681 gctgatataa ggcgccacag caagtttgct gctgaggaac atgaaccgag tagcccaatt
     4741 gattcaatac tgcaatcgtt gataaatgcg tttctacatc gattaatcgc gcaatgcacc
     4801 ctaaaaacaa accgacttca ccgcgttgtt tcccaaccgc aggatagaat gattgccagt
     4861 gctggaaggg ataacggata gggggtaatt tcgatagcaa taatctaaat gaagttccat
     4921 tcagaaaggg caatcgtaac aataagcgct gtaagcccag ttgctggaat aatttaacaa
     4981 atgggcgaag cgtatcaaag cgccagggtt tatcgatcca ttgccgttca agccagcttc
     5041 gtatcttata ggggacatct gcttttttaa ccgatgcatt caaaattttg acccgagctt
     5101 ggtcaactag ctttccgtag gacactttac ttggacatac atattcacag gcccggcaag
     5161 ttaagcaccg atcgatatgc aaggtaaagc gctcattcat gggaatgtgc tgtttcatga
     5221 ccccgctgat catcgcgatg cggccgcgtg gcgaatctgc ttcagacaac gttaatcggt
     5281 aagttgggca atgtggcagg caaagtccgc aggcaacgca tttgtctgct tcagcgagta
     5341 tttcagtttg attccaggat gcaactgaag cttgcggttt ttctaatcgg gtcgtttcca
     5401 tgaataaaaa ttgataatgt tttattgtta ttgccaattg ctgcttacaa ataggtttag
     5461 gttgattgta gccgatttat tagcacgtaa tagtacacgc ttcatcagtc gtaattcagc
     5521 gctacagatc attttactga gcattgagct atcgtatcga atctaactct gattgcccat
     5581 cgaaactcat ttatgctgta cttggtccca aagcaggaac ttgctcctca ggggtttgtt
     5641 tatgattaca atttgtgggt atatcaaagt ttgttatgat gaaagctaag taaataacac
     5701 aaatacagag aatagcgtgc ggtttactga gttagatttc atttttgacg taatggcttg
     5761 tatcattccg aacgtctgtt tttagctagg catttatgaa tgcagcagag cgcatcgctg
     5821 agaagtacct gcaacacatg gggattgaac ttgtagagtt tgagcctgat ggaaatgtca
     5881 cccctgattt tcttgttaac aagaaaatcg cagtggaggt tcgaaggttc aaccaaaatt
     5941 acgaggatac gactgggaac acactcggac tggaggaaac agctatcccc ctggctcaaa
     6001 aattcaaaca gctattgaaa tcacttggac catctacaaa tggggagact tggttcgtta
     6061 ccattgattt cagtagacct atagagcctt ggttaatcct tcgccccaag attgaagtgg
     6121 cactgaatgg tctcaggcta gacgcctcct ttaagcagag aatcatacag gtaaacgact
     6181 tagttgagct ttacctcatc cgagcaagca aggatcatgg aagcgccttc gtatttggtg
     6241 gttacagcga cggagactca ggtggtttgg ttatggcaga ggtagaaaga aatctcagaa
     6301 tctgcattgc cgaaaaggag aagaaagttg cgccacaccg acacaagtac ccggaatggt
     6361 ggctgcttct cgttgattac atcgacttcg gaatggagga agatgaccgc gtcgtgttta
     6421 aagagtcagt catgccgcgc ataaagcact ctttcgacaa ggttatcttt gtcaatccac
     6481 tggatcatcg ctatgggttt gaagtttaac tagatgctat agtgttcaat tactacgttt
     6541 tcttagctaa aacgtaagat gatgagtcga gctgactgtg caggggttcc atcactcacg
     6601 atagtcctta tttgcgcatc ttatctttga tatattttgc taaaagaaat cctgcgacca
     6661 atgcaatcac tgcataaaga accgtttcaa ataaacccat aaagttcata acttgctaat
     6721 tccatttcaa cagaagaaaa agggatcata ccgcacatga aaacctttcg ggtacgaaac
     6781 gttaaatttt gaggaggcgt tagaatgggg cttggattaa tatgtccgtt acttctctgt
     6841 ttatagcctg gttagtatgg cgatgcttgc tcaaaaaaat tgatgactgc atccaagccg
     6901 acatgattga ttgagaatgt cgtttttccc tgcagaatcg gttttccatg gaaggcaaca
     6961 ctggttcctg cttcggtcaa catcggtaag tcgtttgcgc catcgccgat cgcgataact
     7021 tgctctttgc ttaaaccgag gtgatctcga atttctttta aaatatctgc ttttcctttt
     7081 gcaccaatga tttctccgac tactttacct gtcaatttgc catcagcaat ttccaattga
     7141 ttggcaaacg aaaaatccag acctaatttt gctttgatgc gatcggtgaa gaacgtaaat
     7201 ccaccggaga tcaccatggt ttttaatcca gctaatttca ttttttgcaa catcttatcg
     7261 gcaccgagag agagctttac tttctcatca taaacttgct gcaataagtt ttctgggagt
     7321 gctttgagta gtgcggtccg ccggatcaaa ctttcagcaa aatcgatttc accttgcatg
     7381 gtgctggcgg taatttcagc aacttgcggc ttaacatcgc acaacgcagc gatttcatcg
     7441 atggattcga tggcaatgag cgtggagtcc atatccataa cgaccagacc aaactcgctt
     7501 agttttttgt tggggacgac ataagcaaaa tcaagctgta cgtctgcaca ataatcggga
     7561 atttcactgt gttcacttgc attgaccaaa cggaatgctt ctcccgtgat gcgctcaata
     7621 tgcgaggctt gagctagttt tgcgatatgc cttaggtcat gattttggat atcaagacct
     7681 tgaatgatta aattcataaa atttctgagt atagattaaa ttttttgcag agatagctta
     7741 ggcgttacac acgcaaattg gcaagttgtg tgtataactg aaatgcgaga ttgaatgata
     7801 ctaatttttt ggagcgatac taactgtgat tttgcaggga gagtgaatga tcccattcga
     7861 caattttatt gcgacccgca tgcttagctg catatagcgc taaatcggcg gcttccaaca
     7921 tgccatcgtg ggaagtaaaa atactccctt tatcaagatt cgtggcaatg cccatcgata
     7981 cggttatccc aatgtgctta tcattgtcga gcttatagga gatggcttca atactatttt
     8041 ttagtcggat aagcagtttg cgcgctgagg caatgggtgt tccgggtaga atgaggacaa
     8101 attcttcacc cccatagcga cataacaaat catcttggcg aatctgtcca aaaatggctc
     8161 tgaccagtgc gatgagcaat gcatctccca ccacgtgtcc atggctatca ttcacctgct
     8221 tgaaatgatc gacatcgatc attgcgatgg ttagcggggt tgagtgttgg gtagaaaaga
     8281 taaattcacg ttcaaagact gaatcgaaga aagcgcggtt atgcgctcca gtcaatccat
     8341 cgcgttgtgt tttgtcttcg agatcacgta tttttattaa ggattgaatc gataacaatt
     8401 cttgagcttc cgacatgagc gcattgagct cactttcact gcagatacta aattcaaata
     8461 attcctcgac cgttctgagc tcatctttga tttttgcgat aactttgagt aaagcttgac
     8521 catctaagtt aagtcgagca tatagcttag tcatggtttg cgctattttt tcggtatcgc
     8581 ttgggtttaa aaaataatct gcgatatacc ccgatgcggc gacgcaatcg gctatcgttg
     8641 gttctccctt ttgagaagaa gattgattat ggctcgtcat gcaagcgatt gccacatagc
     8701 tcggtaaatt ccagcgtttg agtaaggtat agcctagctc gtcatgcccc gtttcaaacg
     8761 cttcgtattc tgcctgaagc aaagcatcat gggaggcggc ggatgaatag attttggcat
     8821 acgcttcagg aatgatcgag aagaaggcga ggataccgat atcttgtaat aaaccagcaa
     8881 gaaacaaatc atccgccatt ttcaagttaa ttttgttgcc tagcgcttta catgcaagtg
     8941 ctgcaatgat cgatctacgc caaaaaagat cactactttc ttttccgata ctggattctg
     9001 tgtgctgcat caatgttttc gtcagcgaga atgaaagcgc gatgctgatg acggcatgag
     9061 tacctaatac acaaaccgcc tgacggatgt tgctagatgt tcttctcgat ttatacaatg
     9121 gggaattagc agtttttagt atttttgctg ctaatgccgg atctagcgca atgtagtgac
     9181 taatttggat catgtcagta tcagcactat tagctagatc gataatcttg agtgcgaccg
     9241 caggtaggct cggtaatgtc gagcaaaaac gcagttgttg gatgagattc tctttcatta
     9301 ccttccctct atcgcctata aaaattgaga atagttaatt tttcctttag tagtcataat
     9361 gcattctaag cgatgcaacc taatgaaaag aaagaataag aaggcaaaaa atgctagagt
     9421 ttgtcattat gaataaaatt cgatttgaat tgtttggctt cttttgactt tgtaccggga
     9481 gtccagcgat aatccaattg aattgctatc gaataactga caaggagtgg aatgtggatt
     9541 tcgcaatcaa ggtaggaagc gctgacaagc aacgcagtgc ttgtatcgtg gtaggtgttt
     9601 ttgaatcgcg taaactgacg aacgcagcta aacatatcga tgtcgctacg gatggttatg
     9661 taagtaatgt ccttcgtact ggagatatga atggaaaact gggcactacg ctactattgc
     9721 ataatattcc taccagtctg agtaaacgta ttttactagt cggtttaggc caagaaaagg
     9781 aatgcaatgg taaagcgttt agaacggcaa ttttgtcagc gatgagtgcg ctgctgcgta
     9841 ctgccgtatc ggaagtcggt ttgtttttgc tggatgtttc cttgaaagat cgtgatctat
     9901 cctggaagat atctcaaaca gcaatgcttg cgagtgagtg tgtttatcga ttcgatgcac
     9961 ttaaatccaa atcagagggt gagcctccgt cgttgaataa agtaacgttc tatctcggtg
    10021 accgagcata cctcaaaata gctgagatag ctttggcaca ggggaaagca attgctcagg
    10081 gtatgaaagt agctaaagat ttgggtaatt tagcacccaa tatttgtact cctagctatc
    10141 ttgccaaatg cgctgttgat atgggtaagt ctcacaaatt gaaggtctca gtactcggtc
    10201 agaaagagat ggagaagctg aagatgggtg cgctattggc tgtggcaaaa gggagtcacc
    10261 aaccggctaa attaatcgca ttggagtacc gagggctcgc caaaacagaa aaaccgatcg
    10321 tattggtcgg taaagggatt acctttgata ccggcggtat ttcactcaaa ccggcagcgg
    10381 aaatggatga gatgaaatat gatatgagtg gtgccgccag tgtgttggga actttggctg
    10441 cagtcgctga aatgcagttg ccgctcaatg tagtgggtat tatcccaacc accgaaaata
    10501 tgcctggtgg taatgcgact aaaccgggag atgtcgtgac cagtatgtct gggcaaacta
    10561 tcgaaatact gaatacagat gcagaaggac ggttgattct atgtgacgca ttaacctatg
    10621 cagagcgtta caatcctgaa gcagtcattg atatcgctac attgacgggc gcttgtgtca
    10681 ttgcactcgg tcatgtcgct tctgggttaa tgagtaacaa tgatcaattg gcggatgaat
    10741 tattgcaagc tgctgagtta tcggtagatc gcgcttggcg attaccttta tgggatgatt
    10801 accaagactt attaagaagc aattttgcag atatggcgaa tattggtgga agatcggcag
    10861 gaacgatttc tgcagcttgt ttcctggcga gatttgctga gaaataccaa tgggcgcatc
    10921 ttgatattgc aggtactgct tggaagtctg gaaaagaaaa aggttctacc ggacgtccag
    10981 ttcctttact cacgcaattc ttaatttcac gggcgatgcg aaaagtggca cataactgag
    11041 gctaggctac cgaagcgact tgaatgcaat tggatcttgg caaatctagc gcacctaatt
    11101 caatcgtagt tagatcagca tgattagcag gaatggtaca agttgatttt tatacaggtg
    11161 cggcagataa gctttcaact gcctgccgtc tatgtataaa ggccgttcag caaatgcaga
    11221 gaatgcttat actgagtgat gatgcaccgc aactcactca gttggataaa ctgctctgga
    11281 ctttttcttc ggtcagtttc attccacatt gtttcgtgga caatcaacta tctgcgatta
    11341 ccccgatcgt attgggttct ccagcccatg tttatcctga agcgttaaat tatggtattt
    11401 tgcttaattt tcatgatgaa attttgcctt actatcactg ctttgatcga gttattgaga
    11461 tcgttgatca tactgcagcg gataaagaga aagcgcgtaa acgataccgc atctatcaag
    11521 cgcaaggctg tcaggttcga caccataact tagatggttg attgacaatg aatagggata
    11581 ttatttgatg actgatcatc tcgatgtgcc acctgaacca gaagataata cgctgctcaa
    11641 taaactcgat gcgatattga agaaatatcg aaaagaatat tctgcggtga tccgtccggc
    11701 ggatcttaaa gcgacgcctg cgataccaga acctccaact cccatcgtaa ccccagcccc
    11761 aactcagaat cagtctgatg tactacccag ttttactgtt ccagaaattc caattttaac
    11821 cgagcgaatc gtcgttgctc cggaaatatc atttaatgag tcgggtattt cgttactcct
    11881 ctgctatgcg tttgattcag cgcttaagga tgcaaaactt gatctgacct caacacagcg
    11941 ttttgagctg cttcaagcac ttgcccagcg gttacctaaa aatctttgag agaatcggtg
    12001 cgctttcttt cgttataatc tctaaattca tagtctgctc tatttatatt atttatggaa
    12061 cttgcaaaaa gttttgatcc aagtgaaatt gaagcacgct ggtatcctga atgggaatcg
    12121 gcagggtact ttcagccgtt gaatgaaggg ggcgtaacac cttactgcat tatgctgcca
    12181 ccacccaatg tgactgggac gttgcatatg gggcatgcat ttcaacatac gttgatggac
    12241 gcattgacgc gttatcaccg gatgttaggt cagaaagtgt tgtggcaacc cggaaccgat
    12301 cacgcaggaa tcgcaacgca aattgtggtt gagcgacagc tcgatcagca gaatttggat
    12361 cgacgcagtc ttgggcggga aaaattcctt gaacgggtct ggaaatggaa agaagaatcc
    12421 ggttcaacga ttacccggca aatgaggcgt atgggaactt cctgtgactg gtcacgtgaa
    12481 cggtttacca tggacgaaac gctttcaaag gcagttacag aagttttcgt gcagttatat
    12541 cgggaagggt tgatttatcg gggaaaacga ctcgttaact gggaccccgt tttacaaacg
    12601 gcggtatctg atttggaagt gatatcagcg gaagaagaag gttcgctttg gctgattcgc
    12661 tacccgtttg agcaacaaga tggaaacgcc gcgctcaccg acggattgat tgttgcaacc
    12721 actcgacctg agacgatgtt gggggatatg gccgttgcag tgaatcccga cgatgatcgc
    12781 tatagccatt tgattgggaa gcacttacgt ctgccacttt ctgagcgaac aatcccgatt
    12841 attggggata gttatgtgga tcctactttt gggacgggtt gcgtaaaaat tacgcccgcg
    12901 catgatttca atgattacca gatgggactg cggcataagc tagtcccgct caatatcttt
    12961 acgcttgacg gtaaaattaa tgagttcgca ccttccgagt atcaggggtt ggaccgattc
    13021 gatgcccgaa aaaggatctt agcggacctt aaagcgcatg ggttattggt tgaaacacgc
    13081 cctcataagc taatgatacc gagaggcgat cgcactcaat cggtgatcga gcccatgttg
    13141 actgatcagt ggtatgtggc gatggaggac atggcgaaac gcggccttga tgtagttgcc
    13201 caaggtgaag tcaaatttgt tcc
//
LOCUS       DAOQLJ010000054        13186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1765_length_13186_cov_5.474678, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000054 DAOQLJ010000000
VERSION     DAOQLJ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 13186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13186
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1765_length_13186_cov_5.474678"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /gene="dnaB"
                     /locus_tag="PK522_05420"
     CDS             complement(<1..1295)
                     /gene="dnaB"
                     /locus_tag="PK522_05420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNC41206.1"
                     /translation="MAQSSIPISITHNELLDIYKTPPHSIEAEQSVLGGLMLDNQAWD
                     KIADIITIADFYRHDHQLIYHHICNLIEQNKPADVITVAESLDNSAELQNIGGLAYIG
                     SIVQSTPTAANIRRYAEIVRERAIMRKLAEVSTQIAESAYNPTGRSATDLLDNAESKI
                     FEIAEAGARGKQGFIDIQPLLKQVVERIELLYSKENPSDVTGIASGFHDLDQKTSGFQ
                     PGDLIIVAGRPSMGKTAFSLNIAEHVALELSKPVAIFSMEMGGAQLALRMLGSIGRLD
                     QHKVRTGRLDDDDWPRLTHALGKLNDAPIFIDESAALNALELRARARRLYRQHGELGL
                     VVVDYLQLMSSHSQNENRATEISEISRSLKGLAKELQVPLIALSQLNRGLEQRPNKRP
                     IMSDLRESGAIEQDADVILFIYRDEVYNPDTQDKGVAEI"
     gene            complement(1416..1871)
                     /gene="rplI"
                     /locus_tag="PK522_05425"
     CDS             complement(1416..1871)
                     /gene="rplI"
                     /locus_tag="PK522_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNC41207.1"
                     /translation="MQIILLEKIGNLGQLGDIVNVKKGYARNYLIPQGKAKWATESAI
                     AEFNTKRAELEKKQAEKLGSAQALAAKLNGLLLQVTQKSGVDGKLFGSVTSSNILESL
                     EAQGFSVEKSMIRMPEGQIKQIGDYPITIAIHHDVLAQITVSVLGETTA"
     gene            complement(1892..2173)
                     /gene="rpsR"
                     /locus_tag="PK522_05430"
     CDS             complement(1892..2173)
                     /gene="rpsR"
                     /locus_tag="PK522_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNC41208.1"
                     /translation="MARFFRNKDNNLKAKEKLANRPLFKRKKFCRFTAEGIKTIDYKD
                     IDILKDFINENGKIIPARITGTKTFYQRQLNTAIERARFLSLLPYTDQH"
     gene            complement(2199..2495)
                     /gene="priB"
                     /locus_tag="PK522_05435"
     CDS             complement(2199..2495)
                     /gene="priB"
                     /locus_tag="PK522_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HNC41209.1"
                     /translation="MNCNLTTICGKLIKLNQIRYTPAGIEVIEFEINHNSTQTEASIH
                     RQITCVITAIAMAHLAKTVSSLTIETSVKLTGFLAKKSKISSQLVLHVSQIDVI"
     gene            complement(2509..2940)
                     /gene="rpsF"
                     /locus_tag="PK522_05440"
     CDS             complement(2509..2940)
                     /gene="rpsF"
                     /locus_tag="PK522_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNC41210.1"
                     /translation="MRHYEIVLIIHPDQSEQVPAMIERYSSMITSKSGKIHRVEDWGR
                     RQLCYIIQKVHKAHYIIMNIECDQETLSDLEHTFKFNDAVLRHLTIRMKAAVTTPSVM
                     MHEGKAKSTLIEDEEDSVSEETGETSEVSDQKSPDQAVNQP"
     gene            3332..4393
                     /gene="hemE"
                     /locus_tag="PK522_05445"
     CDS             3332..4393
                     /gene="hemE"
                     /locus_tag="PK522_05445"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNC41211.1"
                     /translation="MTKLINDTLIRALLRQPTEYTPVWLMRQAGRYLPEYNQTRARAG
                     DFLSLCKNPNFATEVTLQPLARFPLDAAILFSDILTIPDAMGLGLYFSEGEGPRFERP
                     LREEWEIRALTVPDPGVHLRYVIDAVSQIRKALNGQVPLIGFSGSPFTLACYMIEGGG
                     GTDFRQIKTMLYQRPDLLHHILSVNAQAVTDYLNAQIEAGAQAVMIFDTWGGALSHEA
                     YQAFSLQYMKQIISQLKREKDGVRVPSIIFTKGGGLWLEQMVDSRCDAIGLDWTIQLA
                     DARKRVQDKVALQGNIDPAVLFATPEVIKHEVAKVLASYGKGSGHIFNLGHGISQFTP
                     PENAFALVEAVHSLSRQYH"
     gene            complement(4487..4804)
                     /locus_tag="PK522_05450"
     CDS             complement(4487..4804)
                     /locus_tag="PK522_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNC41212.1"
                     /translation="MNGTIAMKRIEIVVTEEELENLIKLLNSAGVRGYSYLKEVGGLG
                     SRGVRNPNDVIWEEENAIVILACKEDQAQKIVDILRPKLKEFGGMCLVSDCLWIEGPA
                     VSY"
     gene            complement(4954..5904)
                     /locus_tag="PK522_05455"
     CDS             complement(4954..5904)
                     /locus_tag="PK522_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HNC41213.1"
                     /translation="MGPTIQPVVLFFVMGVIAGVIKSDLKIPEALYKSLSIFLLIAIG
                     LKGGVSLSKYDIMEVLPTSLALVFSAALIPLAAYPILRFLGKFNQADSGAIAAHYGSV
                     SAVTFAVGLAFMDGIKEYYEGYMPLFLALLEVPALVTGTVLALMGAGGKKTEWGKLMH
                     EVLTGASIYLLMGAICIGYYAGSKNIAPLDKLYMDLFIGALSLFLLEMGLLAASRIGD
                     VIRKGPFMLVFGICFPLAAGLLGAFLGWAFGLSPGGTMVLAVLYASASYIAAPAAMRI
                     AVPAADPAMSIGLSLGITFPFNIFIGVPIYWEMAKWFHGV"
     gene            6475..6687
                     /locus_tag="PK522_05460"
     CDS             6475..6687
                     /locus_tag="PK522_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41214.1"
                     /translation="MSRKVIIGLTAILFLISLSVIPMVLADELSFNWLIVDCVIILTT
                     YVALIYPEVFNAVKRSTKQFKMRYFA"
     gene            7096..8445
                     /locus_tag="PK522_05465"
     CDS             7096..8445
                     /locus_tag="PK522_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNC41215.1"
                     /translation="MATNISSDSGYESNDKSQKSQSLTSQYGSAAFQSQYFFRDTAAG
                     LITGAMAIPLSIGIAIMSDYPIKVGLATVAFASLIGWLFAWFRPGNYIGSPGIAAGLA
                     PVLALGVASFGWENMAFCIFMTAVMQAIIWKFNWQRYLLVAVPVYLVEGLLAGVGLKI
                     FLKFFEFTYEIPAEMVTEVFWNEARIQMVAISAAGVATFLYLFAKFKDKQPAIPYFVL
                     IIGGVILAQFVSVPMIHVEDVDLHFKLPLPADDIAISMLVYMILFCAMLAIVDVIEQV
                     MSNAAIEKIDPLKRKTDSNNSLLAIWVANLGSSFFHGMTNLDGLAKSTTNKLAGAMTK
                     FSVLIIGSVVCFFLFNVHYLDFLPKFALAAIMLFTGYKMVAGLVHVTHYGSYALMLAI
                     ITAGLVFKVGIFEGLLAAMVIHGIIHYMVYTNHNQMPGKEVIRRYFRSLKSDNSNLQ"
     gene            8525..8932
                     /locus_tag="PK522_05470"
     CDS             8525..8932
                     /locus_tag="PK522_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNC41216.1"
                     /translation="MFKRIYVAIDQSEFSKQALIAATKIAQTFKASLCLGHCITDDTQ
                     SNQESALKFLEDTKSQITDIGDIEVKISVADGQYGLNGISTAIAQSANDWQSDLLVVG
                     TSNRKGLDRFFLGSVAEELVSKINCSVMLFRIN"
     gene            8985..9215
                     /locus_tag="PK522_05475"
     CDS             8985..9215
                     /locus_tag="PK522_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41217.1"
                     /translation="MAINKTLLITLMTILATLLGLYMGSISNNGIMIEANENSEVTDC
                     LWIGGALFINYLLLVCYFAPETSPLLKSNSRI"
     gene            complement(9404..10036)
                     /locus_tag="PK522_05480"
     CDS             complement(9404..10036)
                     /locus_tag="PK522_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC41218.1"
                     /translation="MLVDDHAMIRHGLTLLINMENDLEVLCEADDGPEAIKVLKKNFP
                     IDLVLLDITLKTVSGLELMSSILSLYPSLPVLFISMHDESVYAERALRAGARGFVMKQ
                     EPRTILINAIRQVLKGNIFISKNIQDQVLKRIVIRGYGAEATINALTPSEFEILHLIS
                     LGYNTHEISNLLSRSTKTIETHRYNIRTKLKLRDTADLIRYATKWFTDKK"
     gene            complement(10047..12164)
                     /locus_tag="PK522_05485"
     CDS             complement(10047..12164)
                     /locus_tag="PK522_05485"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNC41219.1"
                     /translation="MTITKDDRKISIIGGLLLLGLTLTTGFTVYNAMRQQIETVLDRG
                     LTGSLDGKALLFETRIGKGIEDARALALRPFIIQFMQELNENINNREASNNLDRNVNS
                     LTQIGFSAAVIYDKNDNILSQVGTFSSDENPVITLTSERNAKLIWDNVFVLRVAVDVI
                     NNNNRIGTIVTETKLPNLTRSFMDIQSGGKSVEFLICAKMPEKQLVLTCLIRNPTSVR
                     YAYLDISSKEQNATPKGMALIGKSGVASTLDYRGIPVIEAYTSLPSLGLLMTLKLDQE
                     ELFMPINDKLKDSALYLIILIIAEIILLNWFIGKLITSEKNAQSSKRKAEEYSDELTL
                     KETVLRKKLNEITCLYEIRREMEFEISIDEVCHKILKLLPPALQFPKTSTVEIIFNNK
                     RFTSKDLDEESKTEIGQTPSYIEILETPKIANRKSIFNIYIQSNIYIDIENQGHLRVY
                     YIENVPSSLTEEQIFIDAIARDLEKWIALRKLESTLISVADDQVHKIGQELHDNLGQQ
                     IAAVSFQASALELQIRENKTPHETILELAASIAAQTQGMVTSIKQVSKILLPFELEAN
                     GLITAFYSLTTQISETYNINCVFVTHLKNDRNIDSVVGLNLYRVAQEAINNAIFHGKA
                     KNISISLSITNNELKTKFIHLTISNDGKGINEETIYSSAGMGIKIMQYRAKQLNGKLT
                     ILKHKDGGIEINFIVPILESDKS"
     gene            complement(12262..>13186)
                     /locus_tag="PK522_05490"
     CDS             complement(12262..>13186)
                     /locus_tag="PK522_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC41220.1"
                     /translation="LVAYRIGLHEVRQRELRLILVISLVIVITVVFVVGHMLAGLVVK
                     QVTQLADRVRRLAPGNINGEKLSQPGQDDEIALLARLIDDYQVRITRMLRREQEFTAN
                     LSHELRTPITTIQTSCELLLTEPNLPERTYRRIKMIEGASARMGEHIQALLFLAREQD
                     LGEIELVNITECILEIVDSLQNEISQKQLRFQINVPNNATLKLNRQALNIVLINLIRN
                     AVQYTEEGFITVEFRDRQLSIIDSGIGIEPASMPFLYDRFFRATTQGRGLGIGLAIVK
                     RICDYYEWRIEVDSKPGRGTTFKLTFPLSTF"
BASE COUNT         3883 a         2473 c         2540 g         4290 t
ORIGIN      
        1 ataatttcag caaccccctt gtcttgtgtg tcagggttat atacttcatc acgataaata
       61 aaaagtatta catctgcatc ttgctcaata gcacccgatt cccttaagtc agacataata
      121 ggccgcttgt tgggtcgttg ctctaagcct cggtttagct gtgatagtgc aatcaaggga
      181 acttgaagtt ccttagctag tcctttcaga gagcgtgaaa tttcagaaat ttctgttgcg
      241 cggttttcat tttgtgagtg tgaagacatc aactgtaagt aatctacaac aactaaccca
      301 agctctccgt gttgtcgata aagtctgcgc gcgcgcgctc ttaactcaag tgcattcaat
      361 gcagcgcttt catcaataaa gattggggca tcatttagtt tgcccagagc atgcgttaac
      421 cttggccaat catcatcatc aagtcggcct gttcgtactt tatgttgatc cagtcgacca
      481 attgagccga gcatccttaa agcgagctgc gctcccccca tttccatact aaaaatagct
      541 actggcttgc ttaattctag cgcaacgtgt tcagcaatat tcagagaaaa agcggttttt
      601 cccatagaag gcctgcccgc gacaatgatt aagtcaccag gctgaaatcc agaggtcttc
      661 tggtctagat catgaaaacc tgaagcaatg ccggttacat cacttgggtt ctctttacta
      721 tataagagct cgattcgttc aacaacctgt ttgagtaacg gctgaatatc gataaaaccc
      781 tgttttcccc tagcgccagc ttcagcaatt tcaaagattt tactttcagc attatcgagt
      841 aaatctgtag ctgagcggcc tgttggatta taggcagatt ctgcaatttg tgtgctcact
      901 tcagcaagct tacgcatgat cgcacgttct cgaacaattt cagcataacg gcgaatattt
      961 gctgcagtgg gggtgctttg tacaatagac ccaatatagg ctaatccacc aatattttgt
     1021 aattcagccg aattatctaa agactcggcc acggttatca catcagcagg tttattttgt
     1081 tcgatgagat tacaaatatg atgataaatc agttggtggt cgtgacgata aaaatcagca
     1141 attgtgatga tgtccgctat tttatcccat gcttgattat caagcattaa accacctagt
     1201 acggattgtt cagcctcaat tgaatggggg ggagttttat aaatatctag taattcattg
     1261 tgtgtaatgc tgatagggat tgaagattga gccatgggca ttcatcaaat tcagagatta
     1321 ctaggaagat tattctatca ctgaagcgat agataaaaaa agcagggctg tttgttcact
     1381 aatcagtcct gcttttagaa ataaaagctt ttaagttacg ctgtggtttc gcctagaacc
     1441 gataccgtaa tttgtgccaa cacatcatga tgaatcgcaa ttgtgattgg gtaatcacca
     1501 atttgcttaa tttgtccttc gggcatacgt atcatcgatt tttcaacaga gaagccttga
     1561 gcttctagag attccagaat attcgagctt gtaaccgaac caaataattt tccatcaaca
     1621 ccgcttttct gtgtgacttg taagagtagg ccatttaatt ttgcagcaag ggcttgggca
     1681 gagcctaatt tctcggcttg tttcttttca agctctgctc gtttagtatt aaattctgca
     1741 atcgcgctct cagttgccca ttttgctttt ccttgtggaa tcaagtaatt gcgagcgtat
     1801 ccctttttaa cattaacaat gtcaccgagt tgacctaaat tgccaatttt ttctaatagt
     1861 atgatctgca taatcgttct ccttattgag tttaatgctg atcggtatag ggcaggagcg
     1921 acagaaaacg tgctctttca atagcagtat ttagctggcg ttgataaaag gtcttggtac
     1981 ctgtaatacg cgcaggaatt atcttgccat tttcgttaat aaagtccttt aatatatcga
     2041 tatctttata gtcgatagtt ttaattcctt cagctgtaaa gcggcagaac tttttgcgct
     2101 taaacaaagg gcgattagct aacttttcct ttgctttcag attgttatct ttgtttctaa
     2161 aaaaacgagc catttttctc tttcctgtac ttagataatt aaattacatc gatctgacta
     2221 acatgtagca ctagctgcga gcttatttta ctcttcttgg cgagaaatcc agtaagtttt
     2281 acagaagttt caatcgttaa gctagatact gtcttagcaa gatgtgccat tgctatcgct
     2341 gtgataacac aagtaatctg tcggtggata cttgcttcag tctgcgttga attatgatta
     2401 atttcaaatt caataacctc aattccagcc ggtgtataac gtatttgatt cagtttgatc
     2461 agttttccac aaatcgtcgt cagattacaa ttcaagcgct cgcctgcatt agggctgatt
     2521 cacggcttga tcgggtgatt tctgatcact gacttcgcta gtctctccgg tttcttcaga
     2581 aacgctatct tcttcatctt caatgagtgt ggacttagct ttaccttcat gcatcattac
     2641 agaaggtgtg gtaactgctg ctttcatgcg aatcgttaga tggcgtagta cagcatcgtt
     2701 aaatttaaat gtatgctcca aatcactgag tgtttcttgg tcgcactcaa tattcattat
     2761 aatgtagtgt gctttatgta ctttctggat gatgtagcac aattgacgtc ttccccaatc
     2821 ttctacacga tggattttgc cacttttaga cgtgatcata ctactataac gctcaatcat
     2881 cgctggcact tgctcgcttt gatccgggtg aatgatcaat actatttcgt aatgtctcat
     2941 gaattcctct tgggtaaagc ctcccaagcg aataatctta ttcatagttg gtgaggcaag
     3001 ggttaaaatc aaaaataagc actctatttt actgaatcaa tcagtgaaga tcaaaataac
     3061 ttttgttaac tcagtaaaat agcgaaaaaa tatttcataa atcttgggtg cgaaagcata
     3121 aaatatgcgc agtagcacac atagttttga tgtgatgatg atggtactaa gtatttgcga
     3181 tcgatgaact taaattttcg cggatccatg ttaacccttg gtacattaat attgcaatgt
     3241 agagggatca cacaaataat aaataacaaa gcattattaa tttaaataat gcatcatgat
     3301 tttgatttta tttataaaga tagattgatt aatgacaaaa cttataaacg atacgctaat
     3361 acgcgcctta cttcgtcaac ccactgaata tacacctgtt tggcttatgc gccaggctgg
     3421 acgttatttg ccagagtata accaaacgcg ggcacgagct ggcgattttc tttcactatg
     3481 caaaaatcca aattttgcga ccgaagtgac cctgcagcca ttggcccgat ttccgctaga
     3541 tgcagcgatt ttattttcgg atattctgac gataccggat gcaatgggac taggactata
     3601 tttctccgaa ggtgaagggc cgcgttttga gcgtccgctg cgtgaggagt gggaaattcg
     3661 tgcgcttacg gtaccagacc caggagtaca tttgcgctac gtcattgatg ctgtttcaca
     3721 aatacgtaaa gcattaaatg gacaagtacc tctcatcggc ttttcaggta gccctttcac
     3781 actcgcttgc tatatgattg aagggggtgg cggcactgat tttcgccaaa ttaaaaccat
     3841 gctatatcaa cgccccgatc tgctccatca tatattgagt gttaatgcgc aagcggtgac
     3901 cgattacttg aatgctcaaa tcgaagcggg tgcccaagca gtcatgatct ttgatacatg
     3961 gggcggggcg ctatcgcatg aagcttatca ggcattttct ttacagtata tgaaacagat
     4021 catttcccaa ttgaagcgcg agaaagacgg ggtgagagta cctagtatca tctttactaa
     4081 gggaggggga ttatggctag aacaaatggt tgatagtcga tgtgatgcaa tcggcttgga
     4141 ttggacaatt caattagcag atgcgagaaa gcgtgtacaa gacaaagtcg ctttgcaagg
     4201 taatatagat ccagctgttt tgtttgccac gcccgaagtc atcaaacatg aagttgcaaa
     4261 agtattggcg agttatggaa aaggatcggg gcatatattt aacctaggtc atggtatttc
     4321 ccagtttaca ccaccagaga acgcatttgc cttggtagag gcggttcatt ctcttagccg
     4381 acaatatcac taacgattat aattaatagc gtacaaaaat attcgaggag tgtaatgcgt
     4441 atccatctca cttttgaggc aagatggata cgtaatttga ggaagtttag taagaaacgg
     4501 ctggtccttc gatccacaaa caatcagaaa ccaaacacat cccaccaaat tctttgagtt
     4561 tagggcgaag gatgtcaacg attttctgcg cttgatcctc tttgcaagcc aagatcacaa
     4621 ttgcattttc ttcttcccag ataacatcat ttggattacg aacgccacga gaacctaatc
     4681 cccctacttc ttttagatac gaataaccac gaacacctgc gctattgagt aacttgatta
     4741 agttctctaa ctcttcttcc gtgacgacaa tctcgatacg cttcattgcg atagtgccat
     4801 tcatgacttt ctcctattaa gttattttaa aaaggcctat tttaagagta ttgattgatt
     4861 ctgacttacc ctagcgtaga accctatcta attacaattc agataattac atcaagataa
     4921 gtcgtttaat ctaatcgtag tgacaagctg ttcttatact ccatggaacc attttgccat
     4981 ttcccaataa atgggtacgc caatgaagat gttgaatggg aaagtaatgc ccaatgagag
     5041 cccaatcgac atggcaggat cggccgcagg cacagcaatc ctcatggcag caggtgctgc
     5101 aatgtaagaa gcgctcgcat ataatactgc taataccatg gtgcctccag gagatagtcc
     5161 gaatgcccat cctaagaatg cacccaataa accagcagct agcgggaagc aaatcccaaa
     5221 aacaagcatg aaagggcctt tcctaatcac atcaccaata cgagatgctg ctaacaaacc
     5281 catttctaat aagaataatg ataacgcacc gatgaagaga tccatgtaaa gcttatcaag
     5341 tggtgcaata ttcttacttc ctgcatagta cccaatacag atcgcaccca tgagcaggta
     5401 aatacttgcg ccggtcaata cttcatgcat taatttaccc cattcggtct tcttgccacc
     5461 tgcacccatc agcgctagaa cagtacctgt gacaagagca ggcacttcaa gcaacgctaa
     5521 gaagaggggc atataccctt cataatattc cttaatacca tccatgaagg cgagacctac
     5581 agcaaaggtt acagcactaa ctgaaccata atgtgctgca attgccccag aatcggcctg
     5641 attgaatttt cctaggaatc ttaatattgg ataagccgcg agaggaatca gcgcagctga
     5701 gaataccaac gccaatgatg ttggcagcac ttccataata tcgtacttag aaagtgatac
     5761 gcccccttta agtccgatcg caatcaataa aaaaatactt aaacttttat ataaagcctc
     5821 tgggattttg aggtcggact taatgacccc agctattact cccataacaa aaaacaatac
     5881 tactggttgt atcgttggtc ccatgtgtta ctcccggttt taatttgtaa ttatattaaa
     5941 tagtttcaaa ataaatttat tttacttacc aataacctaa ctttccatac cctctaacgc
     6001 ttggctatca aatatcgttt actgactgat aattttgata attaatcgag aatgatattt
     6061 tcccttagat cgttggcgac agaagctcga gaacaaaaaa atgcctccat atggaaatta
     6121 gcacaaagta cgttaaggga gtgtaatgaa aatgttagga attaataaag tttatgagaa
     6181 gtcctaaata attcatgctt atcaatttaa tctctaagcg aatttatatg aaatgcttcg
     6241 ctatagttag ttatctcagt ttaggcgact attgctgttg gaatgacttc cactatttct
     6301 tgaaatagcc agatggactg gcttttatcg ttatttattc tcaaagtttt tatgtgatga
     6361 taattttgtt agttttgtgt aaattaaaca taaatctatt gtgaactttt tgtgatttgt
     6421 ttgatcttat tggtgtataa ttagattgtt taaaaattaa aggagttatt agctatgtct
     6481 aggaaagtaa taatcggttt gacggcaatt ttgtttttaa tttcattaag tgtaattcca
     6541 atggtgttag ctgatgaatt aagctttaat tggttgattg ttgattgcgt catcatactg
     6601 acgacttatg ttgcgttaat ctatcctgaa gtatttaatg cggttaagcg ttcgaccaag
     6661 caatttaaaa tgcgctattt tgcataatcg acgtaactac tcagcttatt tggcttaaga
     6721 taattttacc tctggtcatt cccagtagta aatattcttt atagtcaaat ggttaagcag
     6781 attagataga caattcggtg aatttcctac cgaattgtct agtaatacaa cctaggaatg
     6841 aatttctgat gtaactttct gatatttata attttctcat caaaagacgt taataacgtt
     6901 caagtaaaat actcaataaa tctaagtaaa agtattagtt tctctaattt ttttcattat
     6961 gaaaattaat actctgctca atatacaaag tatccagcgt agaagtaggc tattcccgta
     7021 gtttggattt aaggatttgc ttctaaattc ttgaatgaat tggttttaat attttttaaa
     7081 acaaggaatt agtctatggc aacgaatata agcagtgaca gtggctatga aagtaatgac
     7141 aagtcacaaa agtcacaaag tttaacgagt caatatggat ctgctgcttt tcagtctcaa
     7201 tacttttttc gtgatacggc cgccggatta ataaccggag cgatggcaat tccattatca
     7261 ataggaatcg ccattatgtc ggactatccg attaaagttg gtttggcgac agtagcattt
     7321 gcatcactaa ttggttggct atttgcttgg ttcaggccgg gcaattacat cggttcacca
     7381 ggtattgcag ctggtttggc gccagtgcta gcacttgggg tagcatcgtt cggctgggag
     7441 aatatggctt tctgcatttt tatgactgcg gtaatgcaag ccattatatg gaaatttaat
     7501 tggcaacgat atttgcttgt tgctgttccg gtatatttag tggagggttt attagctggg
     7561 gttggattaa aaattttctt gaaattcttt gaatttactt atgaaattcc agctgaaatg
     7621 gtaactgaag ttttttggaa tgaagcacgc attcaaatgg tagcaatatc cgcagcagga
     7681 gtagcaacct ttctttatct ttttgctaaa ttcaaagaca agcaacctgc gattccttat
     7741 ttcgtactta ttattggtgg cgtgatactg gcacaattcg ttagtgttcc aatgattcat
     7801 gttgaagatg ttgacttaca ttttaaatta cccttaccag ccgatgatat agcgatatca
     7861 atgttggttt acatgatttt gttttgtgca atgctggcaa ttgttgatgt catcgagcaa
     7921 gtgatgagta atgcagcgat tgagaagatt gatccactaa aacgcaaaac agatagtaat
     7981 aatagtttgt tagcaatttg ggtagctaat cttggctcaa gcttcttcca tggtatgacc
     8041 aacttggatg gtttggctaa aagtacaacc aataaattag ctggtgctat gaccaaattc
     8101 tcagtgctga taatcggttc agtagtttgt tttttcctct tcaatgtgca ttatcttgat
     8161 tttcttccta aatttgcatt ggctgccatc atgttgttta caggctataa gatggtggct
     8221 gggctagttc atgttacgca ttatggttca tatgcactta tgttagcaat tataactgcg
     8281 ggcttagtat ttaaagtggg tatttttgag ggccttctag cagcaatggt gattcatgga
     8341 attattcatt atatggttta taccaatcac aaccaaatgc ctggcaaaga agtcattaga
     8401 agatatttcc gtagccttaa gagcgataac tcaaatttgc agtaaataac tagtctttat
     8461 tagcagcgca gattatttgc gctgctatga gaaatttaat taattatcaa agaaataaat
     8521 aactatgttc aaaagaatct atgttgcgat tgatcaaagt gaattttcca agcaagcatt
     8581 aatagccgct acgaaaattg ctcaaacttt taaggcatcg ttatgtttag gtcactgtat
     8641 cactgatgac acgcaatcta atcaagaatc agccctaaaa tttttagaag acactaaatc
     8701 acaaattact gatatagggg atattgaagt aaaaatttct gttgctgatg ggcaatatgg
     8761 cttgaatgga atcagtactg ctattgctca atctgcaaat gattggcaat cagatttatt
     8821 agtggttggt acttcaaatc gaaaagggtt agatcgcttt tttctgggtt ctgttgcaga
     8881 agagttagtc agcaaaatca actgttcagt aatgctgttc agaatcaatt agtcagtttg
     8941 ctgtgacgta atcaaaatat ttttaatcta aaggaaatat agcaatggca atcaataaaa
     9001 ccttattaat aactttaatg acaattctgg caaccttact aggattgtat atgggttcaa
     9061 tttcaaataa tggaatcatg attgaagcta atgaaaactc cgaagtaact gattgtttgt
     9121 ggataggtgg tgctttattt attaattact tattgttggt ttgttatttc gcaccagaaa
     9181 catctccctt attaaaaagt aactcgcgta tttaacgttc tgctggttgc taatatcaag
     9241 atagataatt ggttaaatca cataggcaag caagtcgtcg acattacttg ctaggtttaa
     9301 tcaattatat ttttcgtgat aggaagatta gttgaatgtt gctcccggtg ctctaagcgc
     9361 catttcctct ccctaactag tccctgctag ttagggtctt tttttatttt ttgtctgtaa
     9421 accatttggt tgcataacga attaaatcgg cggtatctct taattttaat tttgtgcgaa
     9481 tattgtaccg atgggtttca atcgttttag tactacgtga taatagattt gatatttcat
     9541 gtgtgttgta tcctagacta attaagtgca gtatttcaaa ctcactaggt gtcagagcat
     9601 taatcgtagc ttcagctcca taacctcgaa taacaattcg tttcaaaact tgatcttgta
     9661 tatttttgct tataaagata ttacctttta gtacttgtcg aattgcattg attaatatag
     9721 tcctgggttc ttgcttcata acaaagccgc gtgcgccagc tcttaatgca cgttctgcat
     9781 aaactgattc atcgtgcatt gaaatgaata aaactggcaa agaagggtat agtgatagaa
     9841 tgctactcat taactcaagg ccagatactg tcttgagcgt aatatctaac agtactaaat
     9901 cgatcgggaa atttttcttt agtactttga tagcttctgg tccatcatct gcttcacaaa
     9961 gtacctctaa atcattttcc atgttgatca gcaaagtcag cccgtgcctt atcatagcat
    10021 gatcatcaac caacatgatt ttagatttat gatttgtcac tttctaaaat tggtactata
    10081 aaattaattt cgattccacc gtctttgtgc ttaagaattg ttaatttgcc attcaattgc
    10141 tttgcacggt attgcatgat ttttataccc atacctgcag aagaatatat tgtttcctca
    10201 ttaatgcctt taccatcatt cgaaatagta aggtgtataa atttagtctt taattcattg
    10261 ttggttattg atagggaaat agatatgttt tttgctttac catgaaaaat agcattatta
    10321 attgcttctt gagcaactct ataaagattt aaaccaacga cactatcgat attacgatca
    10381 ttttttaagt gagtaacgaa cacgcaatta atattataag tttcactaat ttgagtggtg
    10441 agcgagtaaa aagcagtaat taaaccattt gcttctaatt caaaaggtaa taagattttt
    10501 gatacttgct taatacttgt taccatgccc tgggtctgag cagctataga agctgctagt
    10561 tctagaattg tttcatgggg tgttttattt tcacgaattt gcaattccag agcactcgct
    10621 tgaaaagaaa ctgcagcaat ttgctggcct aaattatcat gaagctcttg accaatttta
    10681 tgaacttgat catctgcgac tgatattaag gtactttcta atttccgtag cgcaatccat
    10741 ttttctaagt cccttgcaat agcatcaata aatatttgtt cttcagtcaa ggaagaagga
    10801 acattttcta tataataaac gcgtaaatgt ccttgatttt caatatcaat ataaatattg
    10861 gactgaatat aaatattaaa gatagatttt ctatttgcta ttttaggtgt ttctagtatt
    10921 tcaatatagg agggagtttg tcctatctca gttttagact cttcatccag atcttttgaa
    10981 gtaaaacgtt tattattaaa aatgatttct acagtacttg ttttaggaaa ttgtaaagca
    11041 ggcggtaaaa gcttaaggat tttatggcaa acttcgtcga ttgagatttc aaattccatt
    11101 tcccgacgta tttcataaag gcaggtaatt tcgtttagtt tcttgcgtaa aacagtttct
    11161 tttaaagtta actcatcgga gtattcttca gccttacgtt ttgagctctg agcatttttc
    11221 tcggatgtta taagttttcc gatgaaccaa tttagtaaga taatttcagc gataattaga
    11281 atgattaagt aaagggcgct atcttttagt ttgtcattga ttggcatgaa caactcttct
    11341 tgatccaact ttaatgtcat taagagccct agactcggta atgaagtgta ggcctcaatg
    11401 acaggaatac cacggtaatc taaggtagaa gcgaccccac ttttaccgat aagcgccatt
    11461 cctttaggag tagcgttttg ttctttagag ctaatatcta gatatgcgta tcgaacagaa
    11521 gtagggttcc taataaggca ggtaagcact agttgttttt caggcatttt tgcacagatt
    11581 aaaaattcaa cactttttcc gccagattgg atatccataa aacttcgagt tagatttgga
    11641 agctttgttt ctgtgacaat cgtaccaatt ctgttattgt tattaataac atcaactgct
    11701 acccttagaa caaagacgtt atcccatatt aatttagcat ttctttcaga agttagtgta
    11761 atcactggat tctcatccga gctaaaagta ccaacctgcg ataagatgtt atcgttttta
    11821 tcgtagataa ccgcagcgct aaacccgatc tgagtaagcg aattaacgtt acggtcaaga
    11881 ttattgcttg cctctcggtt attgatattt tcattgagtt cctgcatgaa ttggataata
    11941 aagggtcgta atgctaatgc acgagcatcc tctattcctt ttccaatcct tgtttcgaac
    12001 agtaaagctt ttccatctaa cgatccggtg agacctctgt ctaatacagt ttcaatttgt
    12061 tgtcgcattg cattgtaaac agtgaagccc gtcgttaatg ttagccctaa taaaagtagg
    12121 ccgccaataa tacttatttt gcgatcatct tttgtgatag tcatgacatt tcctacaatg
    12181 acggtcaaaa acaaaacaaa tcattttcac atctcgaggg gttagcttag gaataaaggg
    12241 gggctttctg aaatacatta tctagaaggt agacaaggga aaggtgagtt taaatgtcgt
    12301 tcctctccct ggtttactat ccacttcaat acgccactcg tagtagtcac aaatacgctt
    12361 gacaatcgca agcccaatac ccagacctcg tccttgagtt gttgcccgga agaagcgatc
    12421 atatagaaaa ggcatagaag cgggttcaat accaattcct gaatcaatga tggataattg
    12481 gcgatctcta aattctactg taatgaatcc ttcttctgtg tattgtactg cattgcgaat
    12541 taagtttatt aatacgatat ttagggcttg tcgattaagc ttgagtgttg cattgttagg
    12601 aacattgatt tggaagcgaa gctgtttctg agaaatttcg ttttgtaatg agtcgacaat
    12661 ctctaatata cattcagtga tattgactaa ttcaatttca cctagatctt gttctcttgc
    12721 taaaaagagt aaggcttgaa tatgctcacc catgcgagca gaagcaccct caatcatttt
    12781 gatacgtcgg taagttcgtt caggtaaatt tggttcggtg agtaatagct cgcaactggt
    12841 ttgaatggtt gtgatgggtg ttcggagttc atggcttaag ttggctgtaa attcttgctc
    12901 gcgcctcagc atacgggtaa ttcgaacctg ataatcatcg attaggcgag caagtaatgc
    12961 aatctcatca tcttgcccag gttgtgagag tttctcgccg tttatattac caggcgctaa
    13021 acgcctaaca cgatctgcta attgagtgac ttgtttcaca acaagacctg caagcatgtg
    13081 ccccacaaca aaaacgactg tgatcacaat aaccaaggat ataacgagga ttagccgtaa
    13141 ttcacgctgg cgaacttcat gtagaccgat tcgataagca actaga
//
LOCUS       DAOQLJ010000055        12955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1821_length_12955_cov_6.167907, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000055 DAOQLJ010000000
VERSION     DAOQLJ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12955
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1821_length_12955_cov_6.167907"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PK522_05495"
     CDS             complement(<1..317)
                     /locus_tag="PK522_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41221.1"
                     /translation="MNNNFIKTVLFIFIVSWSNLAISEQAESAAQVAETQEKADDSEI
                     FGQGWKLVRSPEMGNSGKFVHMVLVDLDKEMDKTVYSAAINRLCGKATDFCRIRFWTQ
                     ERF"
     gene            474..3197
                     /locus_tag="PK522_05500"
     CDS             474..3197
                     /locus_tag="PK522_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNC41222.1"
                     /translation="MKQDWLNPKQININELLKDSNQNTIVLTGNQRLTGVLRGLSEQI
                     FISQQFSVWKSPFIIPWNSWLLMLWEESLISGLLPKYEQLLSPWQEYVIWQEIIKADL
                     SVKPTHSTIQLVQNAWQLVGSWCIPQNESAFSYNQDSQLFWKWLLRFQSICKEKNFVS
                     VVSIANKLQPLFKSQKLISPFYLILTGFDELNPLQVQFLTTLENLGCRIEWVERQTKS
                     NQVFRIACKDVHEEREWMARWVRKKQEDNPGTRIGIVAPQLQNQRDAIQHTLDEILIP
                     EIFHPESQHLTPPYNFSLGKALNQFPPITTALNLLNFFGTTLRFETISHLLRSPLIAG
                     WAQESGARALLDAKLREIGEPVFTVDALIDFCVRFDSNTACPIFLQQLQSTKKWVTVN
                     YNNAKPGQWSSRIDHFLKRMGWLEDYSLSHETFQLIGAWRELLAEFASLDWVMGEMTA
                     IDAISLLSQMASQRIFQSQVNNQAIQILGPLEAQGIEFDFLWVAGLHDSAWPNHFDPN
                     PFIPIAIQRKHQIPHSSGQRELMVTTQQMQRILGSANEVIVSYPQFDEESILRPSPLI
                     KGFTAIDAQDLSLSNVPLWRDIIYAQSQSEIIQQETTLPVETTQVRGGSRIFELQAAC
                     PFHAFAELRLHASPLGKVQMGLNAMERGNLVHRAMEKIWGALNSHQQLMALSEAQLAE
                     LIRTQVMLTIDEIRVDYPYTLTNRIRMIEIARISAQIDKWLALEKTRSPFAVTSREQT
                     VEIELAELKIKLKIDRIDTLVDGRQIIIDYKTGKVSPSEWFGDRPDKPQLPLYSLVLA
                     KQQLAGITFAQLRVDQIAFSGITVEENLLPKVKSFEKMTQIQEIADWPTVIMNWQRVL
                     ENLALQFLSGEATVNPKKYPETCQHCSLQVLCRINEATILSDIEFNPETEA"
     gene            3194..6634
                     /locus_tag="PK522_05505"
     CDS             3194..6634
                     /locus_tag="PK522_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNC41223.1"
                     /translation="MTLRQPIADYEKRLRLLDSQRSFLVQAPAGSGKTGLLIQRFLVL
                     LTKVDTPEEIIAITFTRKAAQEMRQRILQALEQGQSDIVPEDCYVQQTWELAREALRC
                     DRNKDWQLLNNPNRLRIQTIDSLCTMLVGQMPVLSQLGALPRITEDATDLYQEAARRT
                     IEAIESGHEWSDAIAHLIILFDNQLRRLQQLIADMLRRRDQWLPNLVESSHEPEVRQH
                     LEKALTELVEVALQKLVDQAQAINQNEIITLANFAAAHVPNDNPISRCLSLSTLPGSS
                     CRDDRAIWEGIAALLLTQEGNWRKQLTKNEGFPADKESKNKQEKETYSTMKQRMKALL
                     QDLKSAEGFRNQLVQFRALPASRYADSEWETLQALFKLLKLAAAHLEILFQQTGQIDF
                     PALTLAASQALGKPEAPTDLALALDYRIQHLLVDEFQDTSLNQAELLMRLIAGWQVGD
                     GRTLFLVGDPMQSIYRFRQAEVGLFLEIRDRGLFGQIPIEFVRLSVNFRSQQGVIDWI
                     NQHFPNILPNQDEVAMGAIAYTPSIAIHPKMEEQGVTFHPYWQKDEKAEAQQVVSIIQ
                     KTKQADPTQTIAVLVRNRAHLAQIIVQLRQQGLRYQAVEIERLSHRPVIQDLLALTRA
                     LLHQGDRIAWLAILRAPWCGLTLQDMLILSEPVDQVLLDSLRNSTTLARLTEDGQQRL
                     ARVVPIIEGALSQLGKQTISRIVEGAWLALGGPACVMDEIDLQDAAIFFQLLQQLETR
                     GVADALYDSETLQEKVAALYALPNLSEDAAMIQLMTMHKAKGLEFDVVILPGLGYSSK
                     QDDPQLLYWMKHHFQSGQTSLLFAPIDALGDKPNSTTAYLKQITQKKARYETSRLLYV
                     AATRAKHQLHLLGHINHQEDIPDAEMKVPTKDSLLASLWPAVSHQFQITGESLANKST
                     RIANINLNNDFLLQPIQRFRLVKNWQLPTPPAKIQATNIPFFIPETESIEFDWAGETA
                     RLLGKVLHRLLQHIGKMGIEAFKEDDIHKLQQIGRVLLKQSGIMADRFDWAVEQLSTA
                     LNTLWQDEKAKWILSNRHHDIHSEWALTGFRQGELDHIVIDRSFIDAQAVRWIIDFKT
                     GTHRGTDIDDFLGQELQRYRPQLERYAQLLQSMETRTIRLGLYFPILGKWLEWGFKDE
                     "
     gene            complement(6685..7131)
                     /locus_tag="PK522_05510"
     CDS             complement(6685..7131)
                     /locus_tag="PK522_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41224.1"
                     /translation="MTKTPIVLLFFSILTLFTLANSKLADAHRTIIHNRSFSYTPWSY
                     GLGYTTGLYGYRSYYGYYLNPSYGNFPLIYPYPDPFLYPRYRAPIYYSPLINLPLTPP
                     VYIQQSESPPLRENNYWYYCEIPAGYYPDVKDCQTEWIKVPPRLAE"
     gene            complement(7244..8845)
                     /locus_tag="PK522_05515"
     CDS             complement(7244..8845)
                     /locus_tag="PK522_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNC41225.1"
                     /translation="MLEQEVKRRRTFAIISHPDAGKTTLTEKLLMFAGAIHIAGSVKA
                     RKASRYATSDWMEIEKQRGISVASSVMQIEYNDHVINLLDTPGHQDFSEDTYRVLTAV
                     DAALMVIDAANGVESQTLRLLEVCRARNTPILTFINKMDREVRNPLDLIDEIERTLGM
                     DAIPFTWPIGMGKQFRGVFDLQNRCVRVFQPGADRIDAEPELINNFGDDQIATRFGNE
                     FLEAKQEIDLISGASPQFDQSSFLAGEQTPVFFGSAINNFGVREVLDALINLAPPPGP
                     REALQRIVSPQEKKFSGVVFKIQANMNPAHRDRIAFIRICSGEFKRGMNLKVTRNNKE
                     IRTNTVVSFLSQRREILETAYPGDIIGIPNHGTLQLGDTLTEGEILQFTGLPFFAPEI
                     FQTVEINDPMRTKQLKLGLTQLGEEGAIQVFRPHLGSTLLLGAVGHLQFEVVTHRLQH
                     EYGVDARIASAKYQLARWITSDNPKDLQRFIDLNSHRIAYDAVDAPTFLASFEAELSV
                     AKENWPSIHFHKMREHAGLVFQSHA"
     gene            complement(8875..10938)
                     /locus_tag="PK522_05520"
     CDS             complement(8875..10938)
                     /locus_tag="PK522_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC41226.1"
                     /translation="MNPLLEINQLSTVLYTGGNPIRAVDGLSLTIYKGETFALLGESG
                     CGKSMTALSIMRLLPEAGQIVEGQIKLADHDLLQLPEAEMRNIRGKRISMIFQEPMLS
                     LNPVMTISAQIDEVLQHHFDLPNQIKQQRIKELLQQVGISDIERRLSEYPFQFSGGMK
                     QRVMIAMALAGQPQLLIADEPTTALDVTIQAQVLNLMKAIQKNTQMSILLITHDLGVV
                     AKMADRIAVMYAGEIIETARREAFFSNPLHPYSQKLFAALPDKNKRQHGLAVIRGNVP
                     PLSQTFSGCRFVDRCDQAVKQCYEHVPSWTDTNTDHYVKCHLFNQPQQLVSRQTENAI
                     PIARSPSNSPNHELLHVTDLKVHFPIYSGLLKRVSGHVKAVDGISLAISSGKTLALVG
                     ESGCGKTTVGKSILQLIPVTAGSIQFNGIELTKLSPKALRNGRSDYQIIFQDPYSSLN
                     PKMRIFEILKEGIDALGIDKHDTVHRSELQTKLSSSSIETRIDELLQQVNLPAEAKWR
                     YPHEFSGGQRQRIAIARALSVNPKLLVCDEPTSALDVSVQAQILNLLSKLQHDFGLAF
                     LFITHNISVVEYLADEVAVMYLGRIVEKGTVDEVLNHAKHPYTQALLSAVPTINPQPD
                     HAQIELSGELPSLSHTPVGCYFHPRCPRAMPICQQSYPEATVFTATHAAHCHLYEHAV
                     TKAVI"
     gene            complement(10935..12437)
                     /locus_tag="PK522_05525"
     CDS             complement(10935..12437)
                     /locus_tag="PK522_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC41227.1"
                     /translation="MVIPFDPVILWTDAFIYLLIGIVVAISGYIRRHPHLVAPWRRVT
                     HSASGMSAFTILVFFVSIGILDTIHFRPAVESSSHETNTQIFSVEVLSLLDVIATPLH
                     TNVEKTYSAPLSAYLYAKETIELPDGGQVREFARLTFGGAHLVNPETDHLQDITIRSI
                     KGAGWGLFAWSIIIFSITIWLAYQNKTRYWQTQQALWRGQTETPWWAIFITLNVIIVL
                     SGAVIALATHYHVLGTDKVGQDVLYLSLKSIRTGLVIGTLTTLIMLPFALFLGIAAGY
                     FRGWVDDVIQYTYTTLSSIPSVLLIAAAVLMMQVYIETHPDLFETILSRADLRLLFLC
                     IILGITSWTSLCRLLRGETLKLREMEYIQAAHAFAVSHWRIISRHILPNVFHILLIAI
                     VMDFSGLVLAEAVLSYVGVGVDPSMISFGTMINAARLEMAREPMVWWTLLAAFGFMFT
                     LVLSANLFADAVQNAFNPRIRTLPLKTIQPAKLTATQNTISVKQKNIPSI"
     gene            12664..>12955
                     /locus_tag="PK522_05530"
     CDS             12664..>12955
                     /locus_tag="PK522_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNC41228.1"
                     /translation="MNDKLNIAQANQKALALLLSMANRHGLIAGATGTGKTVTLQTLS
                     EQFSQAGVSVFMADIKGDLSGLSQKGGGNSKVEDRIKELGLNQFNYRDNPVVF"
BASE COUNT         3769 a         2658 c         2957 g         3571 t
ORIGIN      
        1 ataaagcgct cttgtgtcca gaatcgaatt cggcaaaaat cggttgcttt tccacataag
       61 cggttaatcg cagcactata aaccgtttta tccatttctt tgtccagatc gactaatacc
      121 atatggacaa atttaccaga atttcccatt tcaggagaac ggactaattt ccagccctgg
      181 ccaaaaatct ctgaatcatc cgccttttct tgagtctctg ccacctgcgc agctgactca
      241 gcttgctccg aaattgctaa attagaccaa gaaacgatga aaataaaaag tactgttttg
      301 ataaaattgt tattcattcc ttctcccgat ttgtattaca aaaaataagc ataacgcgca
      361 attttaccta tttcatttcc aacagtcagc tagcaacatg aaaatttctc atttgctctg
      421 aatcgagtaa gcattctact attgattaat tacgatctct attcagaccg actatgaaac
      481 aggattggct caatccaaag caaatcaata tcaatgagtt attaaaagat tcaaatcaga
      541 acactatcgt tctgacaggt aatcaacgct taacaggtgt tttacgtgga ctgagtgaac
      601 aaatttttat atctcagcag tttagtgtct ggaaatcacc gttcatcata ccttggaata
      661 gttggttgtt aatgctatgg gaagagtcac ttatttcagg actattgcct aaatatgaac
      721 aactactttc tccttggcaa gagtatgtaa tttggcaaga aatcatcaaa gcagatttat
      781 cggttaaacc tactcatagc actattcaac tggttcaaaa tgcatggcaa ttggtgggat
      841 catggtgtat tcctcaaaat gaatcagcgt ttagctataa ccaggacagt caattgtttt
      901 ggaaatggtt attacgtttc caatctatct gtaaggaaaa gaattttgtt tcggttgtca
      961 gtattgcgaa caaacttcag ccacttttca aatctcagaa attaatatca ccgttttatc
     1021 ttatattgac aggtttcgat gaattaaatc cactgcaggt gcagttttta acgaccctag
     1081 aaaatttagg ttgtcggatt gagtgggtag agcgccaaac taaatcgaat caagtttttc
     1141 gaattgcttg caaagatgtc catgaagaaa gggaatggat ggcgcgctgg gtacgcaaaa
     1201 aacaagaaga taatcctgga acgcgaattg gtattgtcgc accacaatta caaaaccaac
     1261 gcgatgcgat tcagcatacg ctggatgaaa ttttaatacc agagattttt caccctgaaa
     1321 gccagcattt gacgccccct tataattttt ccttgggaaa agcgcttaac caatttccac
     1381 cgattacgac cgcactgaat ttacttaatt tttttggcac aacgttacgt tttgaaacga
     1441 tcagccattt acttcgttct ccgcttatcg caggatgggc gcaagagtcg ggtgcgcgag
     1501 cactattgga tgccaagcta cgagaaattg gtgaacctgt atttaccgtt gatgcattga
     1561 ttgatttctg cgtacgattc gatagcaata cagcttgccc catttttttg cagcagttgc
     1621 aatcgacaaa aaaatgggta acagtgaatt ataacaatgc aaagcccggg cagtggtcat
     1681 cgcgtattga tcattttctc aaaaggatgg gctggcttga ggattattca ttgtctcatg
     1741 agacgttcca attaattggc gcctggagag aattactcgc tgaatttgct tcattagact
     1801 gggtcatggg tgaaatgact gcaattgatg cgattagtct cttgagtcaa atggcgagcc
     1861 agcgaatctt ccaatcccaa gtgaacaatc aagccattca aattctaggg ccattagagg
     1921 cgcagggcat tgaatttgat tttctttggg tggcaggatt acatgatagc gcatggccaa
     1981 atcattttga tcctaacccg tttattccca tcgcgataca gcgcaaacac caaataccgc
     2041 attccagcgg gcagcgtgag ttaatggtta cgacgcagca aatgcaacgt atcttaggca
     2101 gtgcgaacga ggtaatcgtg agttaccctc aattcgacga agaaagcata ttacgcccta
     2161 gccctcttat caaagggttt actgccatag atgctcaaga tctatcgttg agtaatgttc
     2221 ctttatggcg agatatcatt tatgcccaaa gtcaaagtga aattattcag caagaaacga
     2281 ctcttcctgt agaaacgaca caggtcagag ggggaagtcg tatctttgaa ttgcaggctg
     2341 cttgcccatt tcacgctttt gccgaattac gcctccatgc ctctccgcta ggcaaggtgc
     2401 aaatggggct aaacgcaatg gagcgcggca atctagtgca tcgagcgatg gaaaagatct
     2461 ggggggcact caactcacat caacaattaa tggctctgtc agaggctcag cttgcagaat
     2521 tgatacgaac acaagtaatg ctaaccatcg atgagattag ggttgattat ccatacacgc
     2581 tcacgaatcg catccgcatg attgagatag cacgaatcag cgcacaaatt gacaagtggt
     2641 tagcattgga aaagacacgt agtccgtttg ctgtgacgag tcgagagcaa accgtcgaga
     2701 ttgagttagc tgaactcaaa atcaaattga aaatcgatcg tatcgataca ttagtcgatg
     2761 gtcgccaaat catcatcgat tataaaacgg gaaaagtaag cccgagcgag tggtttggag
     2821 atcgtcccga taagccgcaa ttaccgctgt atagtcttgt gctggcaaaa cagcaacttg
     2881 cagggatcac ttttgcgcag ttgcgagtcg atcaaatcgc attcagtggc atcacggttg
     2941 aagaaaatct gttaccgaaa gtgaaatcat ttgaaaaaat gacacaaata caagaaatag
     3001 cggattggcc cacagtcatc atgaattggc agcgggtttt ggagaatctt gcactgcagt
     3061 ttttatcggg agaagcaact gtaaacccga aaaaataccc tgaaacttgc cagcattgtt
     3121 cattacaggt gttgtgtcgt atcaatgaag ccacgatatt gagcgatatc gaattcaatc
     3181 cggaaactga agcatgacat tgcgccaacc gattgccgat tatgagaagc gtctgcgatt
     3241 gttggattct cagcggtcgt ttctggtgca agcgcctgca ggctctggta aaaccggatt
     3301 actaatccag cgatttctag tgttattgac gaaagttgat acgccagaag aaatcattgc
     3361 aattactttt acgcgcaaag ctgcacagga aatgcgtcaa agaatcttac aggcgcttga
     3421 gcaggggcaa tcggatatag tgcctgaaga ttgctatgta cagcaaacct gggagcttgc
     3481 tcgagaagcg ctacgctgtg atcgaaacaa ggactggcag cttttaaaca atccgaaccg
     3541 acttcgaatt caaacgattg attcactttg cacaatgctg gtcggtcaaa tgccagtatt
     3601 atcacagtta ggcgctttac ctcgtattac tgaagacgct actgatttat atcaagaagc
     3661 agcaagacgt acgatagaag ccatagaatc cggtcatgaa tggtccgatg ccattgctca
     3721 tttgatcatc ttattcgata atcaattgag gcgcttgcag caactgattg cagatatgct
     3781 gcggcggcgc gatcaatggt tgcccaacct tgtcgaatct tcccatgaac cggaagtacg
     3841 gcaacatcta gaaaaagccc ttaccgaatt ggtggaagtt gcattacaaa aattagtcga
     3901 tcaagcacaa gcgattaatc aaaatgaaat catcacctta gctaattttg ctgcagcaca
     3961 tgttcccaat gataatccta tttcgcgttg tctatcgctt tcgacgttgc cagggtcaag
     4021 ctgcagagat gatcgtgcaa tatgggaagg aattgctgcg ctcttgctga ctcaggaggg
     4081 taattggcgt aaacaactca ctaaaaacga aggattccca gcggacaaag aatcaaagaa
     4141 taaacaagaa aaagaaactt acagcactat gaagcaacgc atgaaagcgt tgttgcaaga
     4201 tttaaaatct gcagaaggct ttagaaatca attagtacag tttcgtgctt tacctgcttc
     4261 acgctacgca gattctgaat gggaaacatt gcaggcatta tttaaattac tcaagctggc
     4321 tgcggcccat ctggaaatac tgtttcaaca aacggggcaa atcgattttc ctgccctgac
     4381 tttggcagca agtcaggcac ttggtaaacc tgaagcgccg accgatcttg cgcttgcatt
     4441 ggattatcgt atccagcatc tgttggtcga tgagtttcaa gatacttcac tcaatcaagc
     4501 tgaattattg atgcgattaa ttgccggttg gcaagtgggg gatggaagaa ctttgtttct
     4561 agtaggcgat ccgatgcagt cgatttatcg gtttcgccaa gccgaagttg gtttgtttct
     4621 agaaattcgg gaccgaggat tattcggtca gattcctatt gaattcgtgc gtttatcggt
     4681 taattttcga tctcaacaag gtgttattga ttggatcaat cagcactttc cgaatatttt
     4741 acccaatcaa gatgaggtgg caatgggtgc catcgcttat acaccttcaa ttgcgataca
     4801 tccgaagatg gaggagcagg gcgtaacttt tcacccttat tggcaaaaag atgaaaaagc
     4861 agaagcacaa caggtcgttt cgattattca aaaaaccaaa caggcggatc caacccaaac
     4921 gattgccgta ttagtgcgca accgagcaca tctggcacaa atcatcgtgc aactgcggca
     4981 gcaagggtta cgctatcagg ctgtcgaaat tgaacgctta tcccaccgtc ccgtcattca
     5041 ggatttactt gcattgacac gtgccctatt gcatcaagga gaccgtatcg cttggcttgc
     5101 gattctgcga gcaccttggt gtggattgac gttgcaagat atgctgattc tgtcagagcc
     5161 cgttgatcaa gtattgcttg attcgctcag aaattcaacg accttagcgc gattaacaga
     5221 ggatgggcag cagcggctag ctcgtgttgt gccgatcatt gaaggtgcct tgagccagtt
     5281 gggtaagcaa acgatcagtc gaattgtgga aggtgcctgg ttagcactgg gtggaccagc
     5341 ttgcgtgatg gatgaaattg atttgcaaga tgcggctatt ttttttcagc tattgcaaca
     5401 acttgagaca aggggagtgg cagacgcgct gtacgacagc gagactttgc aagaaaaagt
     5461 agctgcctta tatgctttac cgaatctatc ggaggatgct gcgatgattc aactgatgac
     5521 catgcataaa gcgaaaggct tggagtttga tgtagtcatt ctgcctggtt tggggtattc
     5581 cagtaaacaa gacgacccgc aattgttata ttggatgaag catcattttc aatcagggca
     5641 gacatcatta ttatttgcgc cgatcgatgc acttggcgat aaacctaact caacgaccgc
     5701 ctacttaaag caaattaccc agaaaaaggc gcgctacgag accagtcgct tgctttatgt
     5761 ggctgcaacg cgtgccaaac accaactaca tttattaggg catatcaatc atcaggagga
     5821 tatccctgat gctgaaatga aggtgcctac taaagatagc ttattagcga gcttatggcc
     5881 tgcggtgagt catcaatttc aaattaccgg tgaatcatta gcgaataaat cgacgcgtat
     5941 agcaaatatc aaccttaata atgattttct cctacaaccc atacaacggt tccgattggt
     6001 aaaaaactgg cagttaccga caccccccgc aaaaatacaa gccacgaata ttccattttt
     6061 cattcccgaa acagaatcga ttgaatttga ttgggcggga gaaaccgcac gtctgctcgg
     6121 taaagtattg catcgcctat tacaacatat tggcaaaatg ggcattgagg cgtttaagga
     6181 agatgatatt cataaattgc aacaaatcgg tcgtgtgcta ttgaagcaaa gtggcatcat
     6241 ggcagaccga ttcgattggg cagtggaaca attatcaacc gcgctcaata ctttatggca
     6301 agatgaaaaa gcgaagtgga ttctgagtaa ccgccatcac gatattcact cagaatgggc
     6361 gctcactggt tttcgccaag gcgaactcga tcatatcgtt atcgatcgca gttttatcga
     6421 cgcgcaggcg gtccgctgga tcatcgattt caaaacggga acacaccggg ggactgatat
     6481 cgatgatttt ctaggccaag aacttcaacg ctatcgacca caactggaac gctatgcgca
     6541 attgctacaa tccatggaaa cacgcactat ccgattgggg ctttatttcc caattttggg
     6601 gaaatggttg gaatgggggt ttaaagatga gtaactgtta tcaatggtag atttactagc
     6661 gctttaaatt tttcgcaata cagttcattc agctaatcga ggtggaactt taatccattc
     6721 tgtttggcag tctttgacat cagggtagta gcctgctggt atttcgcaat aataccaata
     6781 gttattttcc cgcaaggggg gtgactctga ctgttggata taaacaggtg gggtaagcgg
     6841 tagattaatt aaaggtgaat aataaatcgg tgcacggtaa cgaggataaa gaaaaggatc
     6901 gggatacgga taaattaatg gaaagttccc gtaagaaggg ttgagataat atccataata
     6961 gctacgatat ccgtaaagac cggtggtgta gcctaaacca tagctccaag gtgtgtagga
     7021 aaatgaacga ttatgaatga tggtacgatg agcgtcggct aatttactgt tagctagcgt
     7081 gaacaaggtg agaatagaaa aaaataacaa aacaatcggt gttttggtca tttgtttttt
     7141 tgaggtaagc taacgaggcg tggtgctatc atttactata gattaaatag tacatcatta
     7201 gttgcagtga taattgaagg gctattaact tactgcctta atttcaagcg tgggattgga
     7261 aaaccagccc tgcatgttct cgcattttat gaaaatgaat ggaaggccag ttttcttttg
     7321 caacacttaa ttccgcttca aatgaagcga gaaaagttgg agcatcaacg gcatcatagg
     7381 caatgcggtg tgaattcaaa tcgataaagc gctgtagatc ttttggatta tcagaagtaa
     7441 tccagcgtgc taattggtat ttagcagatg caatacgtgc atcgacacca tattcatgct
     7501 gtaagcgatg agtgacgact tcaaattgca aatgaccgac tgcaccgagc agtaacgtgc
     7561 tacccaggtg agggcggaat acttggatag caccttcttc accgagttga gttaatccta
     7621 atttaagttg cttggtacgc attgggtcat taatctccac ggtttgaaat atttccggcg
     7681 cgaaaaaagg aagaccagta aattgcagta tttctccttc agttaaggta tctcccagtt
     7741 gaagggttcc atgattgggg ataccgataa tatcacccgg gtaagctgtc tctaaaattt
     7801 ctcggcgttg tgataagaaa gaaacaaccg tattggttcg aatttcttta ttatttcgtg
     7861 taactttaag attcattcct cgtttgaatt cacccgaaca aattcgaata aaagcaatcc
     7921 gatcacgatg ggcagggttc atattggctt gaattttaaa tacgacacca gaaaatttct
     7981 tttcttgggg tgacactatg cgttgtaacg cttcgcgcgg acctggtgga ggagcaagat
     8041 taatgagcgc atctaatact tcacggacac caaaattatt aattgcggaa ccaaaaaaga
     8101 cgggagtttg ttcacctgct agaaagctgc tctgatcaaa ttggggggat gccccggaaa
     8161 tcagatcgat ttcttgtttt gcctctagga attcatttcc aaaacgagtc gcaatttgat
     8221 catcaccaaa gttgttgatt aattcgggct ccgcgtcgat tcgatctgct ccgggttgaa
     8281 atactcttac acatcgattc tggagatcaa aaacaccacg gaactgtttt cccataccga
     8341 ttggccaggt gaagggaatc gcatccatac ctaaggttcg ttcgatttca tcgatcaggt
     8401 cgagcgggtt tcgaacttct cgatccattt tattgataaa agtgagaatc ggtgtattgc
     8461 gtgcccgaca tacttccaga agccgtaaag tttgtgactc tacaccattg gcggcatcga
     8521 ttaccatcaa ggcagcatca acagcggtga gtactcgata agtatcctcc gaaaaatcct
     8581 ggtgtcctgg cgtatctaat aaattgatta cgtgatcgtt atactcgatc tgcatgacgg
     8641 aactggctac cgaaattccc cgttgtttct cgatttccat ccaatctgaa gtggcgtagc
     8701 ggcttgcctt acgtgcttta acactaccag cgatatggat ggcacccgca aacatcaaaa
     8761 gtttttcagt caacgtggtt ttacctgcat ccgggtgaga gataattgca aaggtgcggc
     8821 gtctttttac ttcctgttca agcattgcgg taagtcctaa aattatttga gttgttatat
     8881 gaccgctttt gttacggcgt gttcatagag atgacagtga gctgcgtgag ttgctgtaaa
     8941 taccgtcgct tcgggatagg attgctgaca aattggcatg gctctcgggc aacgagggtg
     9001 gaaataacag ccaacggggg tatggctcaa cgaaggaagt tcaccactta gttctatttg
     9061 tgcatggtct ggttgtggat tgatggttgg tactgcagat aatagtgcct gagtatacgg
     9121 gtgcttagcg tgatttaaca cttcatcgac ggttcctttt tccacaattc ttcccagata
     9181 cataacagct acctcatctg caagatactc aacgacagag atattatgtg taataaaaag
     9241 aaaggcgaga ccaaaatcat gctgtagttt actcaataaa tttaatattt gagcttgaac
     9301 tgaaacatca agtgcgctcg tgggttcatc acagaccaat aattttgggt taaccgataa
     9361 tgcccttgca attgcgatac gctggcgttg cccccccgag aattcatgtg ggtatcgcca
     9421 ttttgcttca gcaggtaaat tgacttgttg aaggagctca tcaattcgcg tttcaatcga
     9481 acttgaggat agcttagtct gtaattccga acgatgaacg gtatcgtgtt tatcaattcc
     9541 taacgcatcg atcccttctt ttagaatctc gaaaatgcgc atcttgggat taagggaaga
     9601 ataagggtct tgaaaaataa tttgataatc ggatcttcca ttgcgtaatg ctttaggcga
     9661 tagtttggtc agttcaatac cattgaattg aatacttcct gccgtcacag ggataagctg
     9721 taaaatactt ttacccaccg ttgttttacc acaacccgat tcacccacta gggcaagcgt
     9781 ttttccagac gatatcgcta atgaaatacc atcgacagcc ttgacatggc ctgatactcg
     9841 tttcaaaagg ccactgtaaa tcgggaaatg aacttttaaa tccgtaacgt ggagtaattc
     9901 atgattgggg gagttggaag gtgatcgagc gattggaatt gcattttcag tttgcctcga
     9961 aaccagttgc tgaggttgat tgaaaaggtg gcatttgacg taatgatcgg tgtttgtgtc
    10021 ggtccagctt ggcacatgct catagcactg ttttacagcc tgatcacagc gatcaacaaa
    10081 gcggcatcct gagaaagttt gtgataaagg cggtacatta ccgcgaatca cagcaagacc
    10141 atgctggcgt ttgtttttat ctggtaatgc cgcaaatagt ttttgtgaat acggatgcag
    10201 tggattagaa aagaaagctt cacgtcgtgc ggtttcgatg atttcacctg catacatgac
    10261 agcaatgcga tcagccattt ttgctactac accgagatca tgcgtaatga gcaaaatcga
    10321 catctgcgta tttttttgta tggctttcat caaattcaga acttgcgcct gaatggtaac
    10381 atcgagtgct gttgtgggtt cgtcggcgat taataattgt ggttgtcccg ctaatgccat
    10441 ggcaatcatc acacgttgtt tcattccacc agagaactgg aatggatatt cagataggcg
    10501 gcgctcgata tcggaaatac caacctgttg caacaactct tttattcgtt gctgtttgat
    10561 ctgattgggt aaatcgaaat gatgttggag cacttcatcg atttgagctg aaatcgtcat
    10621 aaccggattt aaactcagca ttggttcctg aaatatcatg ctaatccgtt ttccacggat
    10681 atttcgcatc tcagcttctg gtaattggag gagatcgtga tcagctagct tgatttgacc
    10741 ttcaacgatt tgccctgctt caggcaataa tcgcatgata gaaagtgctg tcattgattt
    10801 gccacagcca gattctccaa gcaacgcaaa ggtttcaccc ttatatatcg tcagagaaag
    10861 tccatcaacc gcccgtatag ggttgccacc cgtataaagg acggttgaaa gctggttaat
    10921 ttccagtaac ggattcatat tgaaggaatg tttttttgct taactgaaat tgtgttttga
    10981 gttgcagtta acttagcagg ttgtatggtt ttaagtggta atgtcctaat acgaggattg
    11041 aaagcgtttt gtactgcatc agcaaaaaga tttgcactca aaaccaatgt aaacatgaat
    11101 ccaaatgctg ccagtaatgt ccaccaaacc ataggttctc gcgccatttc aagtcgtgct
    11161 gcattgatca tcgtgccaaa gctgatcatg gatggatcaa ctcccacgcc gacatacgat
    11221 aacacggctt ctgcgaggac taagccacta aaatccataa cgatggcgat taggaggatg
    11281 tgaaatacat tgggaaggat atggcgacta atgatccgcc agtgagaaac tgcaaaggca
    11341 tgtgccgctt gaatatattc catttccctt aactttaatg tttctccgcg tagtagccga
    11401 catagactgg tccagctagt aatacctaag atgatgcaca gaaatagtaa gcgcagatca
    11461 gcgcgagata gaatagtttc gaataagtct gggtgcgttt cgatataaac ctgcatcatc
    11521 aggactgcag ccgcaatcaa gagtacactt ggaatcgaac tcagcgttgt gtaggtatat
    11581 tgaatcacat catcaaccca gccccggaaa taccctgctg cgatgccgag aaacaaggca
    11641 aatggtagca tgatcaatgt agtcagtgta ccgatgacta gccctgttcg tatacttttc
    11701 agtgatagat agagcacgtc ttgaccgact ttatccgtgc ccagcacatg gtaatgggtt
    11761 gcaagggcaa taacagcgcc cgataacacg ataatgacat tgagcgtgat aaaaatagcc
    11821 caccatggtg tctcggtttg gccacgccat agtgcttgct gggtttgcca atagcgagtt
    11881 ttattctgat atgcgagcca aatggtaatg ctgaagatta tgatgctcca agcgaataaa
    11941 ccccatcctg cacctttaat actgcgtata gtgatgtctt gaaggtgatc ggtttcaggg
    12001 tttactaaat gggcaccccc aaaggttaat ctagcaaatt ccctgacctg cccgccatcg
    12061 ggtagctcaa tcgtttcttt ggcgtagaga taggcagata gcggtgcaga ataggttttt
    12121 tcgacattag tgtgcagtgg cgttgcaatc acatcgagca aacttagtac ctcgacacta
    12181 aatatttgag tgtttgtttc atgggaagaa gattcgacag cagggcggaa gtggatcgta
    12241 tctaaaatcc caatggacac aaaaaataca agaatggtaa atgcggacat tccactggcg
    12301 ctatgcgtga ctctgcgcca aggtgctact aagtgtggat ggcgccggat ataaccgcta
    12361 atggcgacaa caattccaat tagtaaataa atgaatgcat cagtccataa aataacagga
    12421 tcaaacggta taaccattta gcgctcagtt gcagtaaatt ggggtgtcga tagtgtgaga
    12481 ataatgcgat attgtagtgg aaatattgat ggagcgtgaa agtgatcgac aacaacggtt
    12541 ttccacagtt ttcgacaata acaaagcttt cctctaatca cataggcaag gctgatttcg
    12601 aatgataaga tttatcaatc aagatacttt tagcgttaag ttctacttgc atagggatga
    12661 attatgaacg ataagctcaa tatcgcacag gctaatcaga aagcgttggc gttgttgttg
    12721 tcgatggcta atcgccatgg attgattgcc ggcgcaactg gaactggtaa aacagtcacc
    12781 ctccagacgt tgtcagaaca gttttcgcag gcgggtgtgt ctgtatttat ggcagatatc
    12841 aaaggggatc tgtcaggatt gagtcagaag gggggaggaa acagcaaagt agaagaccga
    12901 atcaaagagc tagggcttaa ccagtttaat tatagggata accctgttgt ttttt
//
LOCUS       DAOQLJ010000056        12952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1823_length_12952_cov_5.601613, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000056 DAOQLJ010000000
VERSION     DAOQLJ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12952
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1823_length_12952_cov_5.601613"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PK522_05535"
     CDS             <1..401
                     /locus_tag="PK522_05535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC41229.1"
                     /translation="QIQDRLGSLRTLSMTLVIWICALLLAYFTYDRMMFWVVANLVGI
                     ALGSSQSAGRAIIGLFSPPARAGEYFGLWGLATKLAAVIGPLCYGLITYVTQGDHRTA
                     LLVTTCFFMVGLLSLIGIDEKRGRGSAIQD"
     gene            477..965
                     /locus_tag="PK522_05540"
     CDS             477..965
                     /locus_tag="PK522_05540"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNC41230.1"
                     /translation="MDRIVVPQATWMRFAIDDAQRFMGLAEGGPFGACIVKGNEVIAV
                     AHSTVLREHDPTCHAEINAIRQAARALNQHHLNDCVIYSTTEPCPMCFSAIHWARIHT
                     IVFGTRIADVKTLGFNELDLSNEYMKREGHSQVEIVSEFLRDECEQILKTWQKMSHGI
                     AY"
     gene            complement(985..1890)
                     /locus_tag="PK522_05545"
     CDS             complement(985..1890)
                     /locus_tag="PK522_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41231.1"
                     /translation="MDQGNLSFFKLLFIASIILLLAACTTMQSISSTSEPQEPKIGFP
                     AKISLVSLAKEFGTIDETFKVENNECVVIDKEKPENSASLFNKLTDKLIKEGAVNRAD
                     LDEQQIKEAFNLVKSGEFKNDLNDTRDSLIKNIRVFQQSLRTKIKETVTNPQSAFDLI
                     RHSPEYLQINPSNNAQCEQGIKALLTHQNYDNLGTCAPGAVGKIIDKLKTDKTTSAII
                     ETAHIFTNKNNRSSRLAIIAYARMHGIKLTEDDLDKLDQFLDTTNESDLSPLIKVGID
                     KLAEKYGGKDIQAFIRKKDQECSLN"
     gene            2218..2589
                     /locus_tag="PK522_05550"
     CDS             2218..2589
                     /locus_tag="PK522_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41232.1"
                     /translation="MKVINLLVLVVGYVLSASAFAETDLTNASHETLVTYHENIVKEA
                     ELKLAEHKQNLEDYEVHSYYYGRQGQDFQSHETANIEYYEEVITENLKAAELHRKMAS
                     EQNDSIKSANVDNSNVTRTFH"
     gene            2734..4077
                     /locus_tag="PK522_05555"
     CDS             2734..4077
                     /locus_tag="PK522_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNC41233.1"
                     /translation="MKWERWLKVISLLSIFFIAGYPLISRADNLKSFLRSNRIEVGGW
                     IHGGATFNPSQSVGYNGPVIFADQANRFQLNQFNLFLQRSVPSEGKAWHFGGRFDFMF
                     GTDAIFTQAFGVPTFDVNSGEALKRSNWDLDLCCASTKTYGIALPQAYLEALVPVGNG
                     VNIKLGHFYTPTGFETVPAPDNFFYTRAYTLNAGEPFTHTGLLSNYTINPNWSILGGA
                     LTGSANGGWDGGWDKELGNWSGLAGFTWTSDNHATSFHFSGTYGNTSSRSNEVWAFYN
                     VVFKQKLGAKALLVLHHVLGHAGGVLLNNLKYTNVVRDADWMSAVAHLYYDLSENLSV
                     GIRGEWYRDEDGFRNPSPFRIAAATNIVDGTAKSYAGSLSAVTITPADYYDVTVGLSW
                     KAARSLQLKWDAIRKLVVRPNIRYDRVAAYHADTYRPFAGHKDQILFSLDFVLPF"
     gene            complement(4079..4972)
                     /locus_tag="PK522_05560"
     CDS             complement(4079..4972)
                     /locus_tag="PK522_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNC41234.1"
                     /translation="MIEHSHLTIIEALHTHGTLTEAANTLCLSQSALSHQIGYLEKKL
                     GVALWKREGRNLRLTQAGMLLLEVAQQILPVLAQAEKKLTAYREGRQGILRIGVECYP
                     CFEWLTGIIGAFMQSLPDIDVDIIQKFQFTGLEGLQNYHIDVLITPDLIKKENITYET
                     LTEYQLVLVVSIDHPLAGIEKLTPEQLSNEVLLTFPVPQERLDILTCFLTPAHSVPKM
                     LKPIESLEIMLQMTALGRGVCVLPEWLADIKMRSHRLKKIKIGNFGLNQKLYLALRES
                     DQAIPYIRKFIEIGRQSAHHR"
     gene            5075..7357
                     /gene="metE"
                     /locus_tag="PK522_05565"
     CDS             5075..7357
                     /gene="metE"
                     /locus_tag="PK522_05565"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HNC41235.1"
                     /translation="MITTHNLGFPRIGGKRELKFALEQYWSNRISESALLEIAAELRS
                     QHWQYQSKINWMPVGDFSLYDHVLDASFMVGNIPERAQSLAGNELDYYFRVARGRSNA
                     DIGASSCIHAAEMTKWFDTNYHYIVPEFNHATRFCLNAHRLFEQIQQAGKQFTQIKPV
                     ILGPVTYLWLGKSKDDSDKLNLLDDLIEVYAQLFQRLHQSGITWVQIDEPLLVMELPD
                     SWKHALRKTYFQLRATPLKLLLATYFGQLQDNLQLACELPVDGLHLDALSAQDEVAKF
                     VDWLPSHKILSLGIINGRNIWKTDLLDTLDFLEPIADRLQDRLWLAPSCSLLHVPVNL
                     EHEQKLDDEIRSWLSFAVQKLEELNLLAVALNQGRSAVAGELQKNKVAITARQQSDRV
                     NQASVKARMHSANDAMGMRQSPYQQRAVMQQNRFQLPLFPTTTIGSFPQTQSIRRIRQ
                     DFKAGKISDQSYVQAMQREIDCCIQEQEALGLDVLVHGEPERNDMVEYFGEQLNGFAF
                     TEFGWVQSYGSRCVKPPIIFGDVSRSKNITVDWIKYAQSLTKKPVKGMLTGPVTMLNW
                     SFVRDDQPRADTCLQLALAIRDEVLALEQSGIQMIQIDEAALREGLPLRKKQWNSYLD
                     WAIRAFRITANGVKDETQIHTHMCYSEFNDIMQAIAGMDADVITIETSRSSMELLEAF
                     RNFQYPNEIGPGVYDIHSPNIPSVDSIVALLQNAVQRIPKARLWVNPDCGLKTRSWEE
                     VKPALHNMVAATYFCRNMDG"
     gene            7514..7720
                     /locus_tag="PK522_05570"
     CDS             7514..7720
                     /locus_tag="PK522_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion lipoprotein TrbK"
                     /protein_id="HNC41236.1"
                     /translation="MKFKSGILFLGVIGLNIALAGCSGPPTPSPANCSGRGLEVALSN
                     FRGDEVARQAYMDKCDEINAGADK"
     gene            complement(7768..8922)
                     /gene="ppk2"
                     /locus_tag="PK522_05575"
     CDS             complement(7768..8922)
                     /gene="ppk2"
                     /locus_tag="PK522_05575"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNC41237.1"
                     /translation="MVTAKKPTETAVVRKLTRPRRAASGDVGPDNSDKSFEDFAVKEG
                     IAAAQASKAVAVKDIIAHLSGKNTGALPQSLQAIFDGASHDDALALRKALLELHGDAA
                     TKGRRNADDELADDWRDGGYPYKNLLSRKSYEKQKYHLQVELLKLQAWVKETGQRVII
                     LFEGRDAAGKGGTIKRFMEHLNPRGARVVALEKPSEVEQGQWYFQRYISHLPTAGEIV
                     LFDRSWYNRAGVERVMKFCTNDEYLEFMRQAPDFERNLVRSGIHLVKFWFSVSREEQR
                     RRFKEREMHPLKQWKLSSIDVASLDKWDDYTKAKEAMFFHTDTADSPWTVIKSDCKKR
                     ARLNAMRYVLHKLPYSNKKLESIGPLDPLIVGRAHIVYERGEKNNGIALL"
     gene            9189..11450
                     /locus_tag="PK522_05580"
     CDS             9189..11450
                     /locus_tag="PK522_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC41238.1"
                     /translation="MMTSINLFLSTVSAEFRSYRDALRHDLTRPNVTVKVQEDFIVTG
                     TETLDMLDDYIRQCDAVIHLVGDMTGALAQAPSLAWIQSRYPDLAQRLPPLSPCLQPG
                     VPALSYTQWEAWLALYHRKRLIIACPEPNTPRDASYQMIDEQRAQQRQHLERLAAYER
                     YPLRFSSADRLVAEIWRSKLLDLLVHADSIQKSINPPPPKPIILPLASLGTLFKGRDA
                     LLEELQQSLSKAPSAADTPTVVRVLKGLGGVGKTRLALEYARQHVDDYSALLFIVGDS
                     AEALQRNLAALCGLLNLPARQEVDESKQCAAALTWLRQYPGWLLIIDNVDSESAALAV
                     ETLAPQLLGGHVLVTTRLAHWSHHLQPLLVDVLSSEAAVQFLLERTQTERRLLPNDAS
                     EAATLVDELGNLALALEQAGAYICQRRLTFEGYLQQWHRQRDAVLSWYNARQMHYPNS
                     VAVTWQTSFDQLSAPARQLLQCLAWLSPAPVPETLLEVPLADSDNAIDLFAALAELES
                     YSLVSRDDNPSFTVHRLVQEVTRRQQSDLENVHLSEALFWLNTAFSGDPQDVRDWPQL
                     NPLLPHAHAVAEYADLVGITTPTVRLFNQLGVMYLTKSLYDKAEPLMCRALEIDEKSF
                     GAYHPNVAVDLSNLAQLLQTTNRLSEAEPLMLRALKIDEKNFGADHPNVAIRLSNLAL
                     LLKATNRLSEAESLMRRAVIGFIRSLGTTHPNSQVVLNNYIVLLFDLGRTENEIETQL
                     HELMQWLQQNGSS"
     gene            11601..12833
                     /locus_tag="PK522_05585"
     CDS             11601..12833
                     /locus_tag="PK522_05585"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNC41239.1"
                     /translation="MALIVQKYGGTSVGSTERIKHVAQRVAKYHSQGHQVVVVVSAMS
                     GETNRLIALAKEIQSNPNPRELDVMVSTGEQVTVALLSMALMALNIKAKSYTGGQVRI
                     LTDSAHTKARILKIDEEKIRADLNAGSVVVVAGFQGVDEANNITTLGRGGSDTTGVAL
                     AAALKADECQIYTDVDGIYTTDPRIVPEARKLATITFEEMLEMASLGSKVLQIRSVEF
                     AGKYRVKLRVLSSFEEEGEGTLITFEEDNNMEQAVISGIAFNRDEAKITVLGVPDHPG
                     IAYQILGPIADANIDVDMIIQNVGHDGTTDFSFTVHRNELANAMAILKNSILPHIGAR
                     DVIGGDRIAKVSVVGVGMRSHAGIASRMFRVLAEEGINIQMISTSEIKISVVVDEKYM
                     ELAVRVLHKAFELDQVVQ"
     gene            12860..12952
                     /locus_tag="PK522_05590"
     tRNA            12860..12952
                     /locus_tag="PK522_05590"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12894..12896,aa:Ser,seq:gct)
BASE COUNT         3521 a         2709 c         3016 g         3706 t
ORIGIN      
        1 ggcaaattca ggatcgactt ggttcgttgc gaacgttatc gatgacccta gttatctgga
       61 tctgcgcatt gttgctggcc tattttacat atgaccgcat gatgttctgg gtagttgcca
      121 atttagtggg tatcgcatta ggatcaagcc aaagtgctgg gcgtgccatt attggattat
      181 tttctcctcc tgcaagagca ggggaatatt ttggtttatg gggattagcg actaaactgg
      241 cagcagtgat tggcccattg tgctatgggt tgatcaccta tgttacgcag ggagaccatc
      301 gcacggcact gttggtaacg acatgtttct ttatggttgg gttactatcc ttgatcggta
      361 ttgacgagaa gcgtggtaga ggtagtgcaa ttcaagactg atcagcgcaa atcttgattt
      421 tatgtgatcg ttacgttagg gtttttccta aaataatttt gtaatggtgt gatgaaatgg
      481 atcgaatcgt tgtacctcaa gcgacatgga tgcggtttgc tatcgatgat gctcagcgtt
      541 tcatgggatt agcggaagga ggaccatttg gagcgtgtat cgtaaagggt aacgaagtga
      601 ttgccgtggc gcatagtact gtgttacgtg agcacgatcc cacttgccat gcagaaatca
      661 atgccattcg tcaagctgcg cgagcgctta atcaacatca tcttaatgat tgtgtaatct
      721 attccacaac tgagccttgc ccgatgtgtt tcagtgctat tcattgggct agaattcata
      781 cgatcgtgtt tggaacgcgt attgcagatg tcaagacatt aggtttcaat gagctcgatt
      841 tgagcaatga gtacatgaaa cgcgaaggcc atagtcaagt tgagattgtg agtgaatttt
      901 tacgtgatga gtgcgagcag atcctcaaaa catggcagaa aatgagtcat ggcattgctt
      961 attgatgcct tgcctactta cagatcaatt gagactacat tcctgatctt ttttgcggat
     1021 gaacgcttga atatctttgc ctccgtattt ttcggcaagc ttatcgatac ccactttgat
     1081 caacggagac agatcagatt cattcgtcgt gtctagaaac tgatctagtt tatccaaatc
     1141 atcttcggtc agtttgatgc catgcattct tgcataggcg atgatagcaa gtctgctgga
     1201 tcgattattt ttgttagtaa agatatgtgc agtttcgatt attgctgaag ttgttttatc
     1261 cgtttttaat ttatcaataa tctttccaac tgcacctggc gcgcaagtac ctaggttgtc
     1321 ataattttga tgcgtgagta gtgctttaat cccttgctcg cattgcgcat tattactggg
     1381 attgatctgc agatattctg gggaatgacg gatcaaatca aaagcggatt ggggattagt
     1441 gacagtctct tttatctttg ttcttaagct ttgctgaaaa accctaatat tttttatcaa
     1501 agaatctcgc gtatcattta aatcattctt aaattctcct gatttaacaa gattaaacgc
     1561 ttctttgatt tgttgctcat ctaaatccgc gcgatttaca gctccttctt tgatcagttt
     1621 gtcagtaagc ttattaaata agcttgctga attctcaggc ttttctttat caatcaccac
     1681 gcactcattg ttttcaactt taaacgtttc atcaatagtg ccaaattctt tagccaatga
     1741 gacaagtgaa atttttgctg gaaaaccaat ttttggttct tgcggttcgc ttgtagaaga
     1801 tatagattgc attgtagtgc aagcagctag caacaaaata atactggcaa taaacagtag
     1861 tttaaaaaaa gataggttac cttgatccat agaatgctcc tcgttaggta agaagatagt
     1921 gctatgttgg tatctgattg tctagtttgc ggaaatgagt tcccatcgcg ctaataaaaa
     1981 atctattagg ccatatccta atacaataaa gtcattggca caaaaatcta atcaccaatc
     2041 aatgatagga gcctttgcgt taatatttcc aggttgccta ttggctacaa ttcatttctg
     2101 ctatctaagg gaaaccctta gataaaaatc aaattttccc caatatacta actagccttc
     2161 attgaattac gataacacca atggaaaaat tttattaacc tcacggggga tgcagcaatg
     2221 aaagtaatta atctcttagt tttagttgtt ggatatgtat taagtgcttc tgcttttgct
     2281 gaaacagatc ttactaatgc gagtcatgaa acattggtta cttatcacga gaatattgtg
     2341 aaagaagccg aattgaagct tgctgaacat aaacaaaatc tcgaagacta cgaagtacat
     2401 agttattatt atggaaggca aggtcaggat tttcaatcgc acgaaacagc taatattgaa
     2461 tattacgaag aagtcattac tgaaaatctt aaagctgctg aactccacag aaaaatggcg
     2521 agtgagcaaa atgatagtat caaaagtgca aatgttgata acagcaatgt gacgcgcaca
     2581 tttcattaag tacacgaaac ttaattaact aaaaacaaag cgcccttcct ttaccgagga
     2641 agggcgcttt cgcatgtata aaatcttttt tctaatagtg ctaatacgag agattcaagt
     2701 ttctcgagta tttatttttc ttgcttgaat gatatgaagt gggaacgttg gcttaaggtt
     2761 atatcattat tatcaatttt ttttattgct ggctatccac ttatatcacg ggcagataat
     2821 ctaaaatctt ttctgagaag taacagaatt gaagtaggcg gatggataca tggcggtgca
     2881 acttttaatc caagccagtc agttggttat aacggccccg ttatttttgc tgatcaggcc
     2941 aaccgatttc agcttaatca atttaatcta tttttacagc gttccgtgcc ttcggaagga
     3001 aaagcatggc atttcggcgg gcggtttgat tttatgtttg gtaccgatgc catttttact
     3061 caggcgtttg gtgtgccaac ctttgatgtt aattcaggag aagcgcttaa aagaagtaac
     3121 tgggatctag atctatgttg tgcatcaacc aagacttatg gaattgccct tccccaagct
     3181 tatctcgaag cccttgtacc tgttggaaat ggtgtcaata tcaaactagg tcatttttat
     3241 acaccaacag gttttgaaac ggttccagca ccagataatt ttttctatac gcgtgcttat
     3301 acgcttaacg cgggcgaacc gtttacccat accgggttat tatccaatta cacaatcaat
     3361 ccaaactggt ccattctggg tggcgcgctt acaggcagcg ctaatggtgg ttgggatggc
     3421 ggttgggaca aagaattagg aaactggagc ggtctagctg gtttcacttg gacgagcgac
     3481 aatcatgcaa cttcattcca tttttcgggg acttatggca atacctcctc tagatctaat
     3541 gaggtatggg ctttctacaa tgtggtattt aaacaaaaat taggtgctaa agcactatta
     3601 gtattacacc atgtgctcgg gcatgcaggg ggggtgttat tgaacaattt aaaatatact
     3661 aacgtggtgc gtgatgcgga ctggatgagt gcagttgcgc atttatacta tgacttatca
     3721 gaaaatttat cggttgggat aaggggagaa tggtaccgtg acgaagacgg tttccgaaat
     3781 ccatcccctt ttagaattgc ggcagcgact aatatcgttg atggtaccgc caaaagttat
     3841 gcaggaagtt taagtgcagt taccattaca ccggctgatt attatgatgt taccgtagga
     3901 ttaagctgga aagctgcaag atccttacag ttaaaatggg atgcgattag aaaattggtg
     3961 gtgcggccta atatcagata cgatcgagtt gccgcatatc atgcagatac ttatcgcccc
     4021 tttgctggac ataaagacca aattctattc tcattggatt ttgttttacc tttttagttt
     4081 agcggtgatg agccgattgc cggccaatct caatgaattt ccgaatatag ggaatagctt
     4141 gatcggattc ccgtaacgcc agatataatt tctgattgag cccaaaattt ccgattttga
     4201 tttttttaag gcgatgagat cgcattttaa tatcagcaag ccactcgggt agcacgcata
     4261 caccacgtcc taatgcggtc atttgcagca tgatttccag tgattcaatg ggttttagca
     4321 ttttcggtac gctgtgtgct ggcgttagaa aacacgtcaa gatatccagt cgttcctgtg
     4381 gtacggggaa tgtcaacaaa acttcattgg ataattgttc gggtgtcagc ttctcaattc
     4441 cagccaatgg gtgatcaata gaaacaacca gaaccagttg atactcagtc aacgtttcgt
     4501 aggtaatatt ttccttcttg atcaagtcgg gcgtgatgag tacatcgatg tgataatttt
     4561 gtaagccctc caagcctgtg aattgaaatt tttgaatgat atcgacatca atatcgggca
     4621 atgattgcat aaatgcacca atgatacccg tgagccattc gaagcacgga taacattcca
     4681 caccaatccg taaaataccc tgccgaccct ctcggtacgc agtcaatttt ttttctgctt
     4741 gtgccaacac gggtaagatt tgctgagcga cttctagcaa tagcattccc gcctgggtca
     4801 gtcttaaatt gcgcccttcg cgtttccata gcgctacccc caattttttt tccagatagc
     4861 caatctgatg tgataaggcg gattgactta agcaaagtgt attggcggct tccgtaagcg
     4921 ttccatgcgt atgcagcgct tcgataatgg ttaaatggct atgctctatc atgagttaaa
     4981 ctaattgaat ctataaaata tatcattatt tttcatagat tatcactcct agaataagtg
     5041 acttaacttt attctttact ttgagtgata aaagatgatt acaacacata atctgggatt
     5101 cccgcgtatt ggtggtaagc gtgaattgaa atttgcctta gaacagtact ggagtaacag
     5161 gatcagtgaa tcagcgttgc tggaaatcgc agcggaattg agaagtcagc attggcaata
     5221 tcaatccaaa atcaattgga tgcccgttgg cgatttttca ttatacgatc atgtattgga
     5281 tgcgagtttt atggtgggca atatccctga acgagcgcag tcgttggccg gtaacgagct
     5341 agattattat ttccgggtag ctcgaggacg ttcaaatgcg gatattgggg catcatcttg
     5401 tatccacgca gccgagatga cgaaatggtt tgatacaaac taccactaca tcgtgccaga
     5461 gtttaatcat gctacgcggt tctgcttgaa tgcccatcga ttattcgaac aaattcagca
     5521 agcaggcaag caatttacac aaatcaaacc ggtcattcta gggcctgtta catacttgtg
     5581 gcttggaaaa tctaaagatg atagtgacaa gttaaacttg ctcgatgatt tgatcgaggt
     5641 ctacgcgcaa ttatttcaac gacttcatca atcagggatt acttgggtac aaatcgatga
     5701 accgctgctc gtgatggagt tgccagattc atggaaacat gccctcagaa aaacctactt
     5761 tcaattacgt gcaaccccgc ttaagctgct gctcgcaact tattttggcc aactgcaaga
     5821 taatttacag cttgcctgtg agttaccggt cgatggcttg catttggacg ctttgtccgc
     5881 tcaagacgaa gttgccaaat ttgttgattg gttgcctagc cataaaatcc tctcattagg
     5941 catcattaat ggacgcaata tctggaaaac tgatttactg gatacattag attttctgga
     6001 acctattgct gatcgcttac aagaccgttt atggcttgcg ccgtcctgtt cattacttca
     6061 tgtgcctgtt aatctggaac atgagcaaaa attagacgat gaaattcgat cgtggctatc
     6121 atttgcagta cagaaactgg aagaattaaa tctattagcg gtagccctaa accagggtag
     6181 aagtgctgtg gcaggtgagt tacagaaaaa taaggtagca attacagccc gtcagcaatc
     6241 tgaccgtgtt aatcaagctt cggttaaagc gcgtatgcac tccgctaacg acgccatggg
     6301 catgcgccaa tcaccgtatc agcagagagc agtgatgcag caaaacagat tccagttacc
     6361 cttgtttcca acgacaacga ttggctcttt cccgcaaacc caatcgattc ggagaattcg
     6421 gcaagatttc aaggctggga aaatatctga ccaatcctat gttcaagcga tgcaacgcga
     6481 aattgactgc tgtatccaag aacaagaggc gcttgggctg gatgtgttag ttcatggcga
     6541 accggagcgc aacgacatgg ttgaatactt tggtgagcaa ttgaatggat ttgcattcac
     6601 agagtttggt tgggtacagt cttatggctc acgttgcgtt aaaccaccga ttatttttgg
     6661 tgatgtatca cgctcaaaaa atataacggt agattggatt aagtacgcgc agtcattaac
     6721 aaaaaaacct gttaaaggca tgctaaccgg tcctgtcacg atgctgaact ggtcgtttgt
     6781 gcgcgatgat cagccaaggg ctgacacctg cttacaattg gcgttagcga tacgcgatga
     6841 agtattagcg ctggagcaat caggcattca aatgatacaa atcgatgaag cagcattgcg
     6901 tgaagggtta cccctccgca agaaacaatg gaatagctat ctagattggg cgattcgcgc
     6961 ttttcggata actgcaaatg gcgtcaaaga cgaaactcaa attcatacgc acatgtgcta
     7021 ctcggaattc aacgatatca tgcaagcgat tgcgggtatg gacgcagatg ttattaccat
     7081 tgaaacgtct cggtccagca tggagttact cgaagcgttt agaaattttc agtatccaaa
     7141 cgagattggg cctggcgttt atgatatcca ttcgccgaat ataccgtcag tcgattcgat
     7201 tgtagcttta ttgcaaaatg cggtgcagcg gattcccaaa gcgcggttat gggttaatcc
     7261 agactgtgga ttaaagacgc gcagttggga agaagttaag ccagcattac ataatatggt
     7321 tgctgctact tatttttgca ggaatatgga cggctgattt atccggtagt caaaacttat
     7381 ttctcatgcc tactgctgta atcaagcaga ttcaggtaga attctaatgt tagatccgcg
     7441 tgatcgacaa aagtaggcat aatctgatta agtcatcaga ttgtgttgtg tatctcattg
     7501 ggaggaaata ataatgaagt ttaaatcagg aattttattt ttgggtgtga ttggtttgaa
     7561 tatcgcatta gcggggtgca gcggcccacc gacaccaagt ccagcgaatt gttcaggaag
     7621 aggcctggaa gttgcgttat ctaatttccg tggtgatgaa gtagcacggc aagcttatat
     7681 ggataaatgt gacgaaatca atgcaggtgc agataaataa ttaaatagtc tgcctactcc
     7741 agctacctga gcgtagctgg atatctatca caataacgca ataccgttat ttttctcacc
     7801 gcgctcatag acgatatgag cccgacccac aatcagtgga tcgagtggac caatcgattc
     7861 aagtttctta tttgaatacg gaagtttgtg taatacatag cgcatcgcat tcaaacgcgc
     7921 tcgcttcttg cagtcggatt tgatgactgt ccagggtgaa tcagcggtat ccgtatgaaa
     7981 aaacatggct tctttcgctt tcgtataatc atcccatttg tcgagcgacg ctacatcgat
     8041 tgaactgagc ttccattgct tgagggggtg catttcacgt tccttgaatc gacgccgttg
     8101 ttcctcccga cttaccgaga accaaaattt aacgaggtgg atgccacttc gcacaagatt
     8161 acgctcaaaa tcaggtgctt gccgcatgaa ttctaaatac tcatcgttag tgcaaaactt
     8221 catgacacgc tctacccctg cacggttata ccaagagcga tcaaacagaa cgatctcccc
     8281 ggcggtgggt aaatgcgaaa tatagcgttg gaaataccac tgcccttgct ctacttcact
     8341 gggtttttcc aatgcaacca cgcgtgcccc ccgcggattg aggtgttcca tgaaacgttt
     8401 aatcgttcct ccttttcctg cagcatcacg cccctcgaaa agaataatga cgcgctgccc
     8461 ggtttccttg acccacgcct gtagcttgag cagttcaact tgcagatgat atttctgctt
     8521 ctcataggat tttctagaaa ggagattctt atagggataa ccgccatctc gccaatcatc
     8581 tgcgagttcg tcatcggcat ttctgcggcc tttcgttgcg gcatccccat gtaactctag
     8641 caaagccttg cgtaacgcta acgcatcatc atgtgaggcg ccatcgaaga tggcttgcag
     8701 ggattggggt aacgccccgg tattttttcc cgaaagatgc gcgatgatat ctttaaccgc
     8761 aaccgccttg gatgcttgcg ctgccgcaat tccttccttg acagcgaaat cttcaaagct
     8821 tttatcgcta ttatcagggc ctacatcacc tgatgcagcg cgacgtggac gcgttaattt
     8881 tctgacgacc gccgtttcag ttggtttttt tgcagttacc atgttcaatc tccttatttc
     8941 ggatggtatg cagctaaaca tcgcttttga ttgcttgtaa tcattataag atttttatac
     9001 gcataaaaaa caagaaaaga cgtgtcattt tgttaaattc agggagttga agccaaatat
     9061 ttgtgattcc agcgaatttg aaattattat ggcatccgag cgtaaaaata cagaggtatg
     9121 atcgcatggt gttttggcaa atagtaactt actgaatggt aagctgccca taaatcgcaa
     9181 caaaaacaat gatgacatcc attaatctct ttttatccac cgtgagtgca gaatttcgca
     9241 gctatcgcga tgcgttgcga cacgatctta cccgccctaa cgttaccgtc aaagtgcagg
     9301 aagattttat cgtaactggc accgagacat tggacatgct cgacgactac attcgccaat
     9361 gcgatgcagt gatccatcta gtcggcgaca tgacgggggc tttggcgcaa gcgccgtcgt
     9421 tggcatggat tcaatcgcgt tatcccgatc ttgcgcaacg cttgccaccg ctgagccctt
     9481 gtttgcaacc cggggtaccc gcgctatcct acactcagtg ggaagcctgg ctagcgttgt
     9541 atcatcgcaa acgattaatt atcgcctgcc cagaaccgaa tacaccgcgc gatgcttcgt
     9601 atcagatgat cgacgaacag cgcgcgcaac agcggcaaca cctcgagcgc ctagctgctt
     9661 acgagcgtta cccgctaagg ttttccagtg ctgaccgatt ggtagccgag atatggcgtt
     9721 caaaactact cgatcttctt gtccatgctg actcaattca aaagtctatt aatcctccac
     9781 ccccaaagcc tattattctt ccactcgcaa gtctcggtac gctgtttaaa ggtcgcgatg
     9841 cgttattgga agagctgcag caaagtttga gcaaggcacc tagcgctgct gatacaccga
     9901 ctgtggtacg tgtgttgaag ggcctcggtg gggtcggaaa aactcgatta gcgttggaat
     9961 acgcccgaca gcatgtcgat gactatagtg cgttattgtt tatagtgggt gatagcgcag
    10021 aagcgctgca acgcaacctg gcggcattat gtggattact gaatttgcca gcgcgacagg
    10081 aagttgacga gagcaagcaa tgtgctgctg ctctcacatg gttacgccag tatcccggct
    10141 ggttgctgat tatcgataat gttgatagcg aatctgctgc gctagctgtt gagacattgg
    10201 cgccacagtt gttgggcggt catgtgctgg tgaccacccg cttggcacat tggagtcatc
    10261 acttgcaacc attgctagta gatgttttat caagtgaggc ggctgtccaa ttcctgctgg
    10321 agcgcaccca aaccgagcgc cgtttgttgc ccaatgatgc tagcgaggca gccactctgg
    10381 tcgatgaatt gggcaacctc gcattggcgc tggaacaagc tggcgcttat atctgccagc
    10441 gccgcctaac cttcgagggc tatcttcagc aatggcatcg ccagcgtgat gcggtattgt
    10501 cttggtacaa tgcgcggcaa atgcactatc cgaacagtgt cgcggtaacc tggcaaacct
    10561 cgttcgatca gctcagcgct cccgcccggc agttattaca atgcttagcc tggctcagcc
    10621 ctgcgccggt tccggaaacc ttgctcgaag tgccgctagc cgatagcgac aacgcaattg
    10681 acttgtttgc agcattagcc gagctggaat cctattcatt agtgtcacgt gatgataatc
    10741 ccagttttac cgtgcatcgc ttagtgcaag aagtcacgcg tcgccagcaa agcgacttgg
    10801 aaaatgtgca tctgagcgaa gccttgtttt ggctgaatac tgcgtttagc ggtgatccgc
    10861 aagatgtgcg cgattggcca cagttgaatc cgctgttgcc ccatgcacat gctgtcgctg
    10921 agtatgcgga tttagtggga ataactaccc ccacggttcg attgttcaat cagttggggg
    10981 tgatgtattt aactaaatcc ttatatgata aggcggaacc gttgatgtgc cgcgcgctgg
    11041 aaatcgatga gaaaagcttt ggagcgtacc accctaatgt tgccgttgat cttagtaacc
    11101 tggcgcaact gcttcagaca accaaccgct taagcgaagc ggaaccattg atgctccgtg
    11161 ccttaaagat cgatgagaaa aactttgggg cggatcatcc caatgtggcc attcgcctca
    11221 gcaacctggc attactactt aaagcaacca atcgtttaag tgaggcggag tcgttgatgc
    11281 gccgtgcagt gattggattt atccggagtt tgggcacgac gcatcccaac tcgcaggtcg
    11341 tgctgaacaa ctatattgtg ctacttttcg atttaggtag aacggaaaac gaaattgaaa
    11401 cgcagctcca tgagcttatg cagtggttgc agcaaaacgg ctcgtcttaa gcaagtttat
    11461 cgttagttgc cttgtgtaat tcaatcgtaa aatgagcata gtcttctttt caagaaatgt
    11521 gtaaaatcgc tgagttaatg agtcgacggt attaaagcta aaacattttt gttattcaaa
    11581 attctgatcg gtatttttaa gtggcgttaa ttgttcaaaa atatgggggc acgtcggtag
    11641 gcagcacgga gcgcatcaag catgttgcac agcgggttgc caaataccat tcccaagggc
    11701 accaagttgt cgtagtcgtt tctgcgatga gtggtgagac aaatcgttta attgccctag
    11761 ccaaggaaat tcaaagtaac cctaacccga gagaactgga tgtcatggtt tcgacggggg
    11821 agcaagtgac cgttgcactg ctttccatgg cgctgatggc gctcaatatc aaagcgaaga
    11881 gctatacggg tgggcaagtg agaatcctta ccgatagcgc ccatactaaa gcgcgtattt
    11941 tgaagattga cgaagagaaa atccgtgccg atctcaatgc aggctctgtt gtcgtggtgg
    12001 ctggtttcca aggggtagat gaagccaata atattaccac cctcggtaga gggggatcgg
    12061 atacgacagg agttgcgctt gcggcagcac ttaaggctga tgaatgccag atctacacgg
    12121 atgtagatgg aatctatacc accgatccac gcattgtgcc tgaagcgagg aaactagcaa
    12181 cgattacctt tgaagaaatg ctggaaatgg caagtttggg ttcaaaagtg ttgcaaatcc
    12241 gctcagtaga atttgcgggt aagtatcgtg ttaagctgag agtgttgtcg agttttgaag
    12301 aagaaggtga agggacgcta atcacgtttg aggaagataa taatatggaa caagctgtta
    12361 tttcaggaat tgcatttaat cgtgatgaag caaaaataac agtcttaggt gtgccggatc
    12421 acccaggcat tgcgtaccag atcttgggac cgattgccga tgccaatatt gatgtcgata
    12481 tgattatcca gaatgttgga catgatggca caacagattt ttcatttact gttcatcgta
    12541 atgaattagc caatgcaatg gcaatcctta aaaatagtat cttgcctcat attggcgcac
    12601 gagatgtgat tggtggtgat agaatagcta aagtttcggt cgtaggtgta ggaatgcgct
    12661 ctcatgctgg aattgcgagt agaatgtttc gagtacttgc tgaagaaggc atcaacattc
    12721 aaatgatctc gacttccgaa attaaaattt cggtcgtggt cgatgaaaaa tatatggagc
    12781 tagcagttag agtccttcat aaagcgtttg aacttgatca agttgtgcaa taagcagttt
    12841 acgcgtttct aagaaatacg gagagatggc cgagtggtcg aaggcgctcc cctgctaagg
    12901 gagtataggc acaaaaagct tatcgagggt tcgaatccct ctctctccgc ca
//
LOCUS       DAOQLJ010000057        12892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1833_length_12892_cov_6.368700, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000057 DAOQLJ010000000
VERSION     DAOQLJ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12892
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1833_length_12892_cov_6.368700"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3463
                     /locus_tag="PK522_05595"
     CDS             <1..3463
                     /locus_tag="PK522_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNC41240.1"
                     /translation="KNSQFDRDQIVESAINLFGQSIVPNIEIPNIAEQVTLELAEPQS
                     SAGLEPVTIVMAQTGDSELLAVFLEESDDVLSSIAEKLSVCRSNPTDLEALAAIRRGF
                     HTLKGSGRMVKLYDLSEAAWRIEQVMNRWLSERNPATEELLDLLECGRTTIGIWCDSL
                     KKIGTAEIDPTELFELARRLMYASGPEKGGIGEIETEVRRDVATEQIEPLPLATEVQT
                     SIAVGNIKIPHDLFFVFTSEAGKHAETLNAELSDLTENPNAPIKHEFMLAAHTLSSIS
                     RTLGLTYIADIGVILEQYLTQLLRKSGRPDESALALVKETVSILDSMVSSVRNQQQPS
                     DENIETGKRLSSELSTLLEKLQAEETEVADIHYEQDLVDQAISTEQRAFVSSGLRDVS
                     ISDQDKIDLELLEVFIEEAHELLPEIGRNLRLWRAKFEDYSAREGLLRALHTLKGSAR
                     IAGAMALGELLHQMENTVEQTSRENLSVTLFDRLEAEFDEINDHIEQLQRSHTAEAQF
                     APSIIPVTTTAEVPAQSAIRSFNEHVIEAPQSEPIDSEFTTAKTVLRVDSALIDRLVN
                     ESGEASIMRSRVDAELYGFKQSLQDLDESTERMRGQLREIEIMAETRMPSGVITASDN
                     QSAFDPLELDRFTRFQELTRLMAESLDDIVTVHKSLREIHRVTLDAVGQQARLNSQVQ
                     HELIHLRTVPFKHYSEHFYRVVRQVSRDVGKRVNLTIHGDEIEIDRSVLDKINSPLEH
                     LLRNAIVHGIESTDKRLQLGKPEEGQITIDLSRDGNEIILILHDDGAGLDFALIREKA
                     KQLGIADEDTITDEQVKSVLFTHGFTTLQAVNEVAGRGVGLDVVKNEITDLGGQIEVT
                     STLNQGTSFTLRLPITLALAQAVLVKVGEHTYAVPSAQVVHILELNNEGLAAAYQQQS
                     INFQGHCYSLAYLPNLLNITNQSPEIKRHNRILLLQNGDLFLAVHVDTLIGNCEIVVK
                     NSGLQLSQVPGVEGATILGDGSIVLIVNLIKIFQRKGEQALILDSPLERFDLNKQIEK
                     PKLPIVMVVDDSLTVRKVTSRLLEREGYEVIIAKDGVGALQILQEILPSIMLVDIEMP
                     HMDGFELIRAVRNNAEMSKIPIIIISSRTAEKHRKLAAELGVHVFLGKPYQEEELLEH
                     IIKLIHK"
     gene            complement(3527..3718)
                     /locus_tag="PK522_05600"
     CDS             complement(3527..3718)
                     /locus_tag="PK522_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfB family protein"
                     /protein_id="HNC41241.1"
                     /translation="MDVSAIAEVATTLSEARTTQAVGIAVLKKAIDQNAENALSLISA
                     LPNLQNLPPHLGKNINVVA"
     gene            complement(3930..4211)
                     /locus_tag="PK522_05605"
     CDS             complement(3930..4211)
                     /locus_tag="PK522_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41242.1"
                     /translation="MFKKSLSLIALLAALFFSINSFAATACSADTEKDGKTACSANEA
                     CYLVEGTCIEKKSLPEGKACKKSGVCTNLCVKDDGTETKSDGSETGKCN"
     gene            4410..4667
                     /gene="rpsP"
                     /locus_tag="PK522_05610"
     CDS             4410..4667
                     /gene="rpsP"
                     /locus_tag="PK522_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNC41243.1"
                     /translation="MVVIRLARGGAKKKPFFSMVVADSRFRRDGRFIERVGFYNPSAK
                     GGEEKLRIQLERINHWKSHGAQLSDTVTRLVKQFGSQQQAG"
     gene            4712..5224
                     /gene="rimM"
                     /locus_tag="PK522_05615"
     CDS             4712..5224
                     /gene="rimM"
                     /locus_tag="PK522_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964743.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNC41244.1"
                     /translation="MGHIIGPHGILGCVKVACYTEKITALLDYPTWWIGSDSKADIWH
                     EIGVKSYFVHADRLVVLLENITDRTEAIKLKGAQIAIPKSQLPVLPENGDAGYYWVDL
                     IGTQVVNIQNEVLGRVVGLLETGANDVLRVKDDTKDSKERLVPFIDHVVIQVDLKSKK
                     ILVDWGLDYC"
     gene            5237..6010
                     /gene="trmD"
                     /locus_tag="PK522_05620"
     CDS             5237..6010
                     /gene="trmD"
                     /locus_tag="PK522_05620"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNC41245.1"
                     /translation="MSLEIDVVTLFPEMFDAVTKYGVTGRAEKNKVYQLNTWNPRDYA
                     EDSRKSVDDSPYGGGQGMVLLAAPLEKAIVNARARLKSIGIDDAHVIYLSPQGKLLDH
                     EKVLELSGYGSLILVCGRYEGVDERFITRYVDEEISIGNYVISGGELAAMVLIDSIVR
                     HLPGSLGDNNSAKEDTFSNLLLEFPQYTRPEIYNGEPVPSVLLSGNHAKIKRWRLQQS
                     LGKTWLNRPDLLAQKCEEGLSKEEELLLEEFKQQQDYKS"
     gene            6040..6426
                     /gene="rplS"
                     /locus_tag="PK522_05625"
     CDS             6040..6426
                     /gene="rplS"
                     /locus_tag="PK522_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNC41246.1"
                     /translation="MNLIKQFEQEEIERLGKVIPNFNPGDTVQVNVNVVEGDRKRVQA
                     YEGVVIAKRNRGLNSSFIVRKISSGEGVERTFQTYSPMIASIEIKRRGDVRRAKLYYL
                     RNLSGKAARIREKLTVRTEAVVDDKK"
     gene            complement(6511..7350)
                     /gene="nadC"
                     /locus_tag="PK522_05630"
     CDS             complement(6511..7350)
                     /gene="nadC"
                     /locus_tag="PK522_05630"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNC41247.1"
                     /translation="MQIDEIRKNVAIALAEDVGSGDLTAQLISNDANIAAQVISREAA
                     ILCGIAWFEACFNQLSSDIAIHWLVKEGDILQPNQTLCKIEGNARTILTAERSALNFL
                     QFLSAVATQTHQYVKAVSGTNALIVDTRKTIPGLRYAEKYAVRCGGGANHRIGLYDGI
                     LIKENHIIASGSIAQTLHKARTIAPTETMIEIEVETLDELHQALHAGATMILLDNFEL
                     ETLRNAVILNQQYTQPAILEASGNITLTNVRSIAETGIDRISIGSLTKNIKAIDLSMR
                     FLH"
     gene            7752..8915
                     /locus_tag="PK522_05635"
     CDS             7752..8915
                     /locus_tag="PK522_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HNC41248.1"
                     /translation="MGYLGIELIGTLLIGFLLTKFPGNKDLLLKFLILVYCISPFLIL
                     IFSLVISKLPDEYYFLGYLAFLAWKYSAYYYIVLQIQDQNKVMGLMITCGLFITAYPL
                     IIHSHTLTFWYEDYYQTSEQPIYTGDVLNYVNQEQLYYDQYQLLHNSLDLIHPGVLGK
                     SEIFFVGFGSDATQDVFMKEAKHIQNVSDAYLGTNDRSMVMINNIATMDTVPLATSTN
                     LALSLAHISRKMNLEEDVLFLYLTSHGSGNHELSVNMWLLDLNDIRPEDIKAYLDEAG
                     IRWRVILISACYSGGFIQKLQNENSLIFTAAAADKTSFGCVNENEFTYFGETVFDQIK
                     REPYSLETSFKMAIEKIKQRELSENLPHSEPQLFIGNMMLDKLKQLEQVARFP"
     gene            9218..9382
                     /locus_tag="PK522_05640"
     CDS             9218..9382
                     /locus_tag="PK522_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41249.1"
                     /translation="MSLIVVGNALGVKEGRGAYDKTIVFFVCFGRNGKAFTKIGPDTS
                     SYIASHYPCA"
     gene            complement(9554..10132)
                     /locus_tag="PK522_05645"
     CDS             complement(9554..10132)
                     /locus_tag="PK522_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC41250.1"
                     /translation="MNAKRIIALAGLSGAGKTTLIRQVVDESKVVHLSASELIKTQIK
                     VENVVDKTSEALRTGNIANNQDRLITAFTAIAPSITEHIILGCHTIIDTPTGIQEIPI
                     EVFKTIKITDFLFLSVDPMELYQRREKDMLRSRPIRTIEELESQQKIGLELAHQIAEA
                     LCIPFFDIGAAPEEQLLSILQSLPLLSKRTPR"
     gene            complement(10132..11670)
                     /locus_tag="PK522_05650"
     CDS             complement(10132..11670)
                     /locus_tag="PK522_05650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC41251.1"
                     /translation="MSSSQKYLIDTNVFIGLEDARQVNAEVASFLALANKHSVGVFIH
                     EAAKDDIARDTDVNRRGISLSKLSKFQILKKVRGLTEQQLEESYGALPKPNDIVDATL
                     LHALDTGAIDFLISEDRGLHARARQHASHLSRRVLFIADAFALLTGTYEPVEVPIRYV
                     DEVGANEIPLTDNIFDSLREGYDGFDEWWRTKCVNKHRRCWVVYDEGLAGLIVRKDES
                     KEDTDATLPGKKILKVCTFKVRPEKRGIKLGELLLKQALWFAQSNKYDIVYLTTQAQQ
                     EALIDLLEYYGFCHTKTLNDKELIFEKFLSRDRVEIDDDEDLYEIAKRYYPRFYTGSR
                     VQAYVIPIKESYHDMLFPELRNNIQGDLLTPIGHGARPTRPGNTIRKVYLCRAPANLT
                     SPGSLLFFYKGKSDLPPSQAITTIGIFEDMQEATSLMELMQLTGGRSVYREDELKKWQ
                     ATTEKPVKVINFLLAGYFSPPLVLGALKTEQIFGSRPPQSIAGLTRTQLDKLFAHLDL
                     GFQT"
     gene            complement(11642..12433)
                     /locus_tag="PK522_05655"
     CDS             complement(11642..12433)
                     /locus_tag="PK522_05655"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC41252.1"
                     /translation="MNLVGKIIETYVGVDGNESLALGKRLQRLRKLAGLTQADFSHRL
                     KISEGALSRLEKRTDIHVSTVKQYVEALGAKLQISAAFSTDNPIAFRIIEAFDAELIN
                     EKQLLLPIFDDDVFRPQRDVVLSIKPQYSSKILKGTKTIELRRRFPIDVPQGTLAFIY
                     STTPEKALIGYTEIVSVTKESVSNIWRSHKIEACISKRDFESYFSGQDFGFALRFRSA
                     KPFKQAIDLTELRERFGFEPPQSFLYVKPELREALRHELLAEISY"
     gene            12721..>12892
                     /locus_tag="PK522_05660"
     CDS             12721..>12892
                     /locus_tag="PK522_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNC41253.1"
                     /translation="MAGHSKWANIKHKKAAQDAKRGKIFTRLIKEITIAARLGGGDSS
                     SNPRLRLAIDKAY"
BASE COUNT         3807 a         2356 c         2791 g         3938 t
ORIGIN      
        1 aaaaaattca caattcgatc gtgatcaaat tgttgagtct gcaataaatc tgtttggcca
       61 atcgatagtt cctaatattg aaataccaaa cattgctgag caagttacgc ttgaacttgc
      121 cgagccgcaa tcatctgcag ggctggagcc tgtcacaatt gtgatggccc agactggaga
      181 ctctgaatta ttggctgttt ttcttgagga atccgacgat gtgttgtcga gcattgctga
      241 aaaattatca gtttgtcgct ctaatccaac agatttagaa gcgcttgcag ccattcgccg
      301 tggctttcat accctaaaag ggagtggtcg tatggtgaaa ctctatgatc tcagcgaagc
      361 ggcttggcgc atagagcaag tgatgaatcg atggttaagt gaaagaaatc ctgctacgga
      421 ggagctactt gatttacttg aatgtggccg tactacaatt ggtatttggt gcgatagtct
      481 taaaaagata ggtactgcag aaattgatcc gacagaactc ttcgaattag caaggcgatt
      541 gatgtatgcc tcagggcctg agaagggtgg tattggtgaa atagaaacag aagtgcggcg
      601 tgatgttgca actgagcaaa tcgagccttt accgttagca actgaagtgc aaacaagtat
      661 tgcggttggg aatattaaaa ttccgcatga tttgtttttt gtgtttacta gtgaagctgg
      721 aaaacatgct gagacactta atgctgaact gagcgatctt acagagaacc ctaatgcacc
      781 aataaaacac gagtttatgt tggcggcgca tacgctctca agtatttcga gaacgcttgg
      841 gctcacctat attgcagata tcggtgttat cttggaacaa tacttgactc agttacttag
      901 gaaatcgggt aggcccgatg aatcagcgct agcactagtt aaagagactg tttctatttt
      961 ggatagcatg gtatcgtcgg ttcgcaatca acagcaaccg agcgatgaaa atattgaaac
     1021 tgggaagcgc ttatctagcg agctatctac tttacttgaa aaattacaag cagaagagac
     1081 tgaagtagct gatattcatt atgaacagga tttggtagat caagcaatct caactgagca
     1141 acgagcattt gtttcatcgg gcctgcggga tgtttcaatc agcgatcaag ataaaataga
     1201 tcttgaatta ctcgaagttt ttattgaaga agcgcatgaa ttgttacctg agattggacg
     1261 taatttaaga ctttggcgag caaagtttga agattatagt gcgcgtgaag gcctgctacg
     1321 agcactgcat acgctgaaag gaagtgctcg tattgcggga gctatggcgc ttggtgagtt
     1381 gttgcatcaa atggaaaata cggtcgaaca gacttccaga gaaaatttat cagttacgct
     1441 ttttgatcgg ctcgaggctg aatttgatga aatcaatgat catattgagc aattacagcg
     1501 atcgcataca gcagaagctc aattcgctcc ttcgattatt cctgtcacta ctactgcaga
     1561 agtaccggcg caaagtgcaa ttcggagttt caatgaacat gtaattgaag cgcctcaatc
     1621 agaaccaata gattctgaat ttacaacggc caaaacagtc cttagggtgg attctgcgct
     1681 tatcgatcga ctggtgaatg agtccggtga agcgagtatc atgcgctctc gcgttgatgc
     1741 agagctatat ggcttcaaac aatccttgca agatctggat gagagtacag aacggatgcg
     1801 gggtcaatta cgtgaaattg aaataatggc tgaaacacgt atgccatcag gggtgataac
     1861 agcttctgat aatcagtctg cgtttgatcc tctagagctt gatcggttta cgcgattcca
     1921 ggagctaaca cgattgatgg ccgagagctt agatgacatc gtgacagttc ataaaagttt
     1981 gcgcgagatt catcgtgtta cgcttgatgc ggtggggcag caggcacgac taaatagtca
     2041 agttcaacat gagttgattc accttcgcac agtaccattt aagcattact ccgaacattt
     2101 ttatcgtgtt gttcgccaag tatcacggga tgtcggaaag agagtaaatt tgacgattca
     2161 tggcgatgaa atcgagattg atcgcagtgt cttggataaa atcaattctc cacttgaaca
     2221 tctgttaagg aacgcgatcg ttcatggtat tgaatcaacg gataagcgtt tgcaattggg
     2281 caaaccagag gaagggcaaa ttactattga tctcagtcgc gatgggaatg aaatcattct
     2341 cattttacat gatgatgggg ctgggctaga ttttgcattg atccgagaga aagctaaaca
     2401 gctgggaata gcggatgaag acactattac tgatgagcag gttaagtcag tgctttttac
     2461 acacggcttt acaactctac aggcagttaa tgaagttgca ggaaggggtg tggggcttga
     2521 cgtcgttaag aatgaaataa cagatttggg tggtcaaata gaagttactt ctacccttaa
     2581 tcaaggaact agctttactt tacgtttgcc aattacgctt gcactcgcgc aagcagtatt
     2641 ggtaaaagtc ggagagcata cttatgcagt accttccgca caagtagtac atattctaga
     2701 gcttaataat gaaggattgg ctgctgctta tcaacagcaa tcgatcaatt ttcaaggtca
     2761 ttgctattca cttgcttatt tgcctaattt attgaatata acgaatcaat caccagaaat
     2821 taagcgccat aaccgaatac tgttgctgca gaatggtgat ttatttctgg ctgttcatgt
     2881 tgataccctt attgggaact gtgagattgt tgtaaaaaat tcgggactgc agttgtccca
     2941 ggttccgggt gttgaaggag ctacaatact cggggatgga agtattgttt taatcgttaa
     3001 tttgataaaa atatttcaac gtaagggaga acaagctttg atacttgatt ctccattaga
     3061 acgcttcgat ctaaataaac aaatagaaaa acctaaatta ccgattgtga tggttgttga
     3121 tgattcatta accgtgcgta aggtaactag ccgtttgctt gaacgtgaag ggtacgaggt
     3181 gataatagct aaagatggtg ttggtgcttt acagattctg caggaaatac ttccatcgat
     3241 aatgctggtt gatattgaaa tgccacatat ggatggattt gaattaattc gagcagttcg
     3301 taataatgcg gagatgtcaa aaatacctat tattattatt tcttctcgaa cagctgagaa
     3361 gcaccgaaaa ttagccgcag aacttggtgt gcatgtattc ttgggaaaac cttaccaaga
     3421 agaagagtta cttgaacata ttatcaaact tatccataaa tgaagcatta ggtaaaagct
     3481 aaatgttatt agactaaaat taataaaatt tagctattac cgctgtttag gcaactacgt
     3541 tgatattctt cccaagatgg ggtgggagat tttgtaagtt tggtaatgct gagattaggc
     3601 taagtgcgtt ttctgcattc tgatcgattg ctttttttag aaccgctatt cctactgctt
     3661 gggtagttct agcttcactc aaagtggttg ctacttctgc aatggctgag acatccataa
     3721 tttgctcctt tgttctagtc cgtatttttt taatcggatc tcagtgcagg atgtttagct
     3781 gtatttatta gagcctgaat cttgatgagg aggaatccgt aaacttgaat tatctgatca
     3841 atcccagttt ggcagtgcgt tttagtctgg gtagtaggat actttgtatc actactaacc
     3901 cccaatccat caattaaacg gaaaattgtt taattgcatt tccctgtttc agaaccgtcg
     3961 cttttggttt cagttccgtc atcttttacg cacaagtttg tgcaaacacc ggatttcttg
     4021 caagcttttc cttctggaag acttttcttc tcgatgcaag taccttcaac tagatagcaa
     4081 gcttcgttcg cactacatgc ggttttacca tctttttctg tatcagcact acaagcagtc
     4141 gcagcaaatg aattaatcga aaaaaataaa gctgcaagta atgcaatcaa gcttaaagat
     4201 tttttgaaca tgattcttcc tcctttgtat tttattaaca aacaaaattt agtttgtgtt
     4261 tgttaattat ctggcctagt gcgcgaaaga tcaagctgtt caatctatag caaaatcaac
     4321 taagtgataa gattatagcg cttgctaaat actatgaggt tatcctaaaa tactgcctcc
     4381 taaggatttt taacgttgga taagattata tggtagttat tcgattagca cgtgggggcg
     4441 ctaagaaaaa gcccttcttc agtatggtgg tagctgattc acgatttaga cgagatggtc
     4501 ggtttattga gcgggttggt ttttataacc cctctgcaaa aggtggggaa gaaaaattgc
     4561 gtatccaatt ggaaagaatt aaccattgga aatcgcatgg agctcagttg tccgatacag
     4621 tcacgcgatt agtgaaacaa tttggtagtc aacagcaagc aggttgagtg agaaaacttc
     4681 tatcgagaca ctagatgaac agttagttcc aatggggcat attattgggc ctcatggaat
     4741 tcttggttgt gttaaggtgg cctgttacac tgaaaaaata accgcactac ttgattatcc
     4801 aacctggtgg ataggtagtg acagtaaagc agacatttgg cacgaaatag gcgttaaatc
     4861 ctatttcgtt catgctgata ggctagttgt tttgctggaa aatattactg atcgaacaga
     4921 agctattaag ctaaaaggag cacaaattgc gattcccaag agtcagcttc ctgttctacc
     4981 ggaaaatgga gatgctggtt attattgggt agatttaatt gggactcagg tagttaatat
     5041 tcagaatgaa gtattagggc gggtagtggg attactggaa acaggtgcta atgatgtatt
     5101 acgcgtcaaa gatgatacta aggatagcaa agaaaggtta gtccctttca ttgatcacgt
     5161 tgtaattcag gttgatctta aaagtaaaaa gattttggtt gactgggggc tggactattg
     5221 ctaaaaggag caagtcttgt cactcgagat tgatgtagtt acactttttc cagaaatgtt
     5281 cgatgctgtt acaaaatatg gagtaacagg gagagcagaa aagaataaag tttatcaatt
     5341 aaatacctgg aatcctcgtg attatgcaga agatagccgg aaatcagttg atgatagccc
     5401 ctatggtggt ggccagggaa tggtgctgtt agcagcgcca ctggagaagg caatagttaa
     5461 tgcgagagct agacttaagt caattggaat tgatgatgcc catgtaattt atctgtcacc
     5521 acaaggtaag ttgctagatc atgagaaagt tctagagttg agcggttatg gtagtttgat
     5581 tttagtatgt gggcgatatg aaggggtaga tgagcgcttc atcacccgat atgtagacga
     5641 ggaaatatca attggtaatt atgttatttc aggcggtgaa ttagctgcca tggtactaat
     5701 tgatagtata gtgcgacatc ttccaggttc gcttggagat aataattctg caaaagaaga
     5761 tactttttcg aatctattgc ttgaatttcc tcagtatact cggcctgaga tttataatgg
     5821 agagccggta ccaagcgtat tattatcggg aaatcatgca aaaattaagc gctggagact
     5881 gcagcagtca ttaggcaaaa cttggttgaa tcgacctgat ttattagctc agaaatgtga
     5941 agaggggctc tcaaaagaag aagagctttt actcgaagaa tttaaacagc aacaagatta
     6001 taaatcttag aatagaaaat aagtgtatag ggagtgtgta tgaatctgat taaacaattt
     6061 gagcaagaag agattgaacg attaggtaaa gtaataccta attttaatcc tggcgataca
     6121 gtacaagtaa atgtgaacgt agtagagggc gatcgaaaac gtgttcaagc gtatgaaggg
     6181 gttgtgattg caaaacgtaa tcgtggtttg aattcttctt tcattgttcg aaaaatatct
     6241 agcggtgagg gagtagagcg aacatttcaa acttattccc caatgattgc gagtattgaa
     6301 ataaaacgac gtggggatgt ccgtcgcgca aaattgtatt atttaagaaa cctatcaggt
     6361 aaagccgcca gaattcgaga aaaacttacg gtgcgcacgg aagcagttgt agacgataaa
     6421 aagtaatctt tctccaatct agaaattaga gaagttgaat actagcgtga gtttcatttt
     6481 ctatctgatt gaataaattt tgattagtag ttagtgcaga aatcgcatcg acaaatcaat
     6541 cgctttgata ttttttgtta aactaccaat agaaattctg tcgatacctg tttcagcaat
     6601 tgagcgcacg tttgttaatg taatattacc tgatgcttcc aagatggctg gttgggtata
     6661 ttgttgattt aatatcactg cgttacgcag cgtttcgagt tcaaaattat ctaatagaat
     6721 cattgttgcg ccagcgtgga gcgcctggtg caattcatcg agtgtttcta cttcaatttc
     6781 tatcatggtt tcagtgggag caatcgttct ggccttatgt agcgtttggg ctatgctacc
     6841 tgaagcaata atgtgatttt ctttaattaa aatgccatcg tataaaccta tccggtggtt
     6901 ggctccgcca ccgcatctta ctgcatactt ttcagcatat cgaaggccgg gaattgtttt
     6961 acgagtatca acaattaacg catttgtgcc tgatactgct ttaacgtatt gatgagtttg
     7021 agtcgcaacg gcagagagaa attgcagaaa attcagtgcc gagcgttccg ctgttaatat
     7081 tgtgcgagca tttccctcta ttttgcataa agtttgattg ggttgaagaa tgtctccttc
     7141 cttgacaagc caatgaattg caatatctga tgatagctga ttgaaacacg cctcaaacca
     7201 agcaatacca cataatattg ctgcttccct actaatgact tgtgcagcga tatttgcgtc
     7261 atttgaaata agttgagcag ttaaatcgcc acttccaaca tcttcagcaa gcgcgattgc
     7321 aacatttttt cgaatttcat cgatttgcat tctgaaaact catgttaatt gatgcttaat
     7381 taaaccatag taatgcaaat ttctgcgata aattgcatat gaagtttatg aactactagg
     7441 atgaagttaa agcctccaac ccaatttatt attgccgttc ataacgctat ctactagaaa
     7501 agttggagat gagaatgaat tggtccacaa gcttacaaaa ggaatcaaaa ttatccacat
     7561 taatcaaaga atttcattca cttttatata atttaaattg tggcctcaga ctattagtat
     7621 ttcgaaatga tcaactaaga tacctaacgg tttcacatga ccaacttttt ttattgctca
     7681 tctgctatgg actaaccgca gttattgcta cctattggat aactgatcgc ccagtattcg
     7741 agtggtatgg tttgggttat ctcgggattg aattaattgg caccctattg attggctttt
     7801 tattgacaaa atttcctggg aacaaagact tattattaaa gtttttgata ttggtttact
     7861 gcatttcacc ttttttaatt ctgatttttt ccttagtcat atcaaaactg cctgacgaat
     7921 attattttct tgggtatctc gcttttctgg catggaagta tagtgcctat tattacatcg
     7981 tattacaaat acaagatcag aataaagtca tgggactgat gataacctgc ggacttttca
     8041 taacagctta tccgttaatc attcattcgc atactttgac tttttggtac gaagattatt
     8101 atcaaacgag tgaacagcca atttacacag gagatgtact taattacgta aatcaggaac
     8161 agctttatta cgatcaatat caacttttac ataattcatt ggatttaatt caccccggag
     8221 tattagggaa gtcagaaata ttttttgtcg gatttggttc agatgcaact caagatgttt
     8281 ttatgaaaga agccaagcat atacagaatg taagtgatgc gtatcttggt acaaatgacc
     8341 gatcaatggt aatgattaac aacatagcaa cgatggatac ggttccctta gcaacatcaa
     8401 caaatttagc actctcactc gcacatatca gtcgaaaaat gaatctagag gaagatgtac
     8461 tgtttttata tttaaccagt catggtagtg gtaatcatga actttccgtc aatatgtggt
     8521 tgctggatct taatgatatt cgtcctgaag atattaaagc atacttagat gaagctggaa
     8581 tccgttggcg tgtcattcta atttctgctt gctattcagg tgggtttatt caaaaactcc
     8641 agaatgaaaa cagtttgatt tttacggctg cagctgcaga taaaacatcg tttggttgcg
     8701 tcaacgaaaa tgaatttact tattttggag aaactgtatt tgaccaaatt aaaagagaac
     8761 cttattccct tgaaacaagc tttaaaatgg caatcgagaa aattaagcaa cgtgaattat
     8821 ctgaaaattt accgcattct gaacctcagc tatttatagg aaatatgatg ctagataagc
     8881 taaagcaatt agagcaagtg gcaagatttc cgtgataact tttagatagt ttagaaaagt
     8941 gatgaattat ttctcggttg ctagtatttc taataatgag aaagctgaat ttgcaccgga
     9001 gatcgcttgt tcagcagtgt tttgatccaa aagatttctc tgtaggagtt ggagtatttc
     9061 tcgccaatga ccttgtgaaa gagttgtaag atgagataaa taattgtgag caccttcaat
     9121 ctgtgctcca agtactttct tgcaatgacg ttcgatgatc tggctaccag agtgagttga
     9181 gaacttaaat cacctttttg aggcatcgtg taagtcgttg agtcttattg tggtaggcaa
     9241 cgcattaggg gttaaagaag gtcgaggtgc ttatgacaaa acgattgttt tttttgtgtg
     9301 ttttgggcgc aatgggaaag catttacgaa gattggccca gatacttcaa gctacattgc
     9361 aagccattat ccatgcgcat gacgcgccag agtactatca tttattctga tggatggcgc
     9421 aactaataac ggattgtggt cgctatcagc tctgatagtc ccttcagggt tcgtcatggc
     9481 agtaactagt tttccagtag caagaggtat ataacagtat cgaatctttc tggagttttg
     9541 cgaaatattc atctcatcgc ggagttcttt ttgagagtaa aggtaaagat tgcagaatag
     9601 ataaaagctg ctcctcagga gctgcaccaa tatcaaaaaa cgggatacaa agagcttctg
     9661 caatttggtg agctaattca agaccaattt tctgttgtga ttccagctct tctattgtcc
     9721 gaattggtcg cgagcgcagc atatcttttt ctcgcctctg atagagttcc attggatcaa
     9781 cactgagaaa aagaaaatct gttatcttta tagtcttgaa aacttctatc ggaatctctt
     9841 gaattcccgt tggcgtatca atgatcgtat gacagcccaa aattatatgc tctgttatgc
     9901 tcggtgcaat tgctgtaaat gctgtgataa gccgatcttg attgttagca atattaccgg
     9961 tgcgtagtgc ctccgatgtt ttatcaacta cattctcaac cttgatttgg gttttgataa
    10021 gctcacttgc tgagaggtga acaacctttg attcatcaac aacttggcgt atcaatgttg
    10081 ttttacctgc accagataac ccagccaatg cgataattct ctttgcgttc attatgtctg
    10141 aaaacctaaa tctagatgtg caaacaattt atccaattgt gttcttgtca aaccagcaat
    10201 ggactgaggt ggccgacttc caaaaatctg ttctgttttt agagcaccca gaacaagggg
    10261 tggtgaaaaa taacctgcaa gaaggaaatt gatgacttta actggttttt cagttgttgc
    10321 ctgccatttc ttcagttcat cctctcgata tacagatcgc cctcctgtca gttgcataag
    10381 ttccattaat gacgttgctt cttgcatatc ttcaaaaata ccaattgttg ttatcgcctg
    10441 cgacggaggt aaatctgatt tacccttata aaagaacaag agagatcccg gacttgtaag
    10501 attggccggg gcacgacaga gataaacttt tcgtattgtg tttccgggac gagtcggcct
    10561 tgcaccatgt cctattggtg tcaatagatc gccttgaatg ttatttctca gctctggaaa
    10621 aagcatgtca tggtaagatt ctttaatagg aataacatac gcttgcactc gggaaccagt
    10681 gtagaatcgt ggataataac gctttgctat ttcatataaa tcttcatcgt catcaatctc
    10741 aacccggtca cgtgaaagga atttttcgaa aatcaattcc ttatcgttaa gtgtttttgt
    10801 atgacagaaa ccataatatt cgagtaaatc aatcagtgct tcttgctgtg cctgcgttgt
    10861 aagataaaca atgtcatatt tatttgactg tgcaaaccaa agcgcctgct tgagtagcaa
    10921 ttctccaagc ttaattccgc gtttctcggg cctgactttg aacgtacaaa ccttgagaat
    10981 ctttttccca ggcaaagtgg catcagtatc ctcttttgac tcatcttttc taacaattaa
    11041 gcccgctaga ccttcatcat aaacaaccca gcagcgtcga tgtttattaa cgcattttgt
    11101 tcgccaccat tcgtcaaatc catcataacc ttcacgcaga ctgtcaaaaa tattatcggt
    11161 taatggaatt tcgttcgcac caacctcatc gacataacga attggaactt cgaccggttc
    11221 ataggtcccg gttaataggg cgaatgcatc agcgatgaac aatacacgtc ttgataaatg
    11281 cgaagcgtgt tgccgtgctc gcgcatgtag cccacgatcc tccgagatga gaaagtcaat
    11341 agctcctgta tccagcgcgt gcagtaatgt tgcatcaaca atatcgttag gcttcggtaa
    11401 cgcaccataa ctttcttcaa gttgttgttc tgtcaggccc cgtacttttt taagaatctg
    11461 gaattttgag agcttactta acgaaatccc ccgacgatta acatcagtgt ctcgtgctat
    11521 atcatcctta gccgcttcgt gaataaagac cccaacgctg tgtttgtttg ctagcgcaag
    11581 aaaggaggca acttctgcgt ttacctgtcg agcgtcttca agaccgatga atacgtttgt
    11641 atcaataaga tatttctgcg aggagctcat gccttagcgc ctcgcgcaac tcaggcttaa
    11701 catacagaaa agattggggc ggttcaaaac caaagcgttc ccttaattca gttaaatcta
    11761 tagcctgctt gaatggtttt gcactcctga atcgcagtgc aaaaccgaaa tcttgaccgc
    11821 taaaataact ttcgaaatct cgttttgaga tacacgcctc tattttatgt gagcgccaaa
    11881 tatttgagac agactccttg gtgacagaaa ctatttccgt ataaccaatg agtgcctttt
    11941 caggcgttgt ggaatagata aacgcaagtg tcccttgagg gacgtcgatg ggaaatcgcc
    12001 gccgcagctc gattgttttt gttcccttga ggattttgga cgagtattgc ggtttgatac
    12061 ttaacactac atccctttgt ggacgaaaaa catcgtcgtc aaatatcggg agcagcaatt
    12121 gtttttcatt aataagttcg gcgtcgaatg cttctataat acggaaagca atagggttat
    12181 ctgtagaaaa tgctgcacta atttgcagtt ttgcccctaa cgcctcgaca tattgtttca
    12241 ctgtggaaac atgaatgtcg gttcgctttt ctaatcggga taatgctccc tcgctaattt
    12301 ttagtctgtg agaaaaatct gcttgcgtta gcccggctag cttacgcaac ctttgcaacc
    12361 gcttgcccag agcaaggctc tcgttgccat caactccgac ataggtctca atgatttttc
    12421 ctactaaatt cattatataa gcttcctctt tctcatagtt tagcatgttc attaacaggc
    12481 aaaaatatgt aaagtcttgg agaaatttca ttttttttat tactctattc cctcaagaaa
    12541 ttgaggcgct tgtaagcttg ggtcgattta aattgcaaat taattattgc atgagcttat
    12601 ttctgatggt tggcgcacaa tttattgccg gcatacttta ttcatgagta atggaatgct
    12661 aggattacag tcttattggt gaagaaccac aatatctagc aaactgagga agaaaatcaa
    12721 atggcgggtc atagtaagtg ggctaatatc aagcataaga aagcagcaca agatgcgaag
    12781 cgcggtaaga tttttacgcg attaatcaaa gagattacga ttgctgcaag gcttggtgga
    12841 ggagactcaa gcagtaatcc acgcttacgg ttagcgattg ataaagcata tg
//
LOCUS       DAOQLJ010000058        12629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1895_length_12629_cov_6.503976, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000058 DAOQLJ010000000
VERSION     DAOQLJ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12629
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1895_length_12629_cov_6.503976"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..78)
                     /locus_tag="PK522_05665"
     tRNA            complement(2..78)
                     /locus_tag="PK522_05665"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Arg,seq:acg)
     gene            273..1307
                     /gene="queA"
                     /locus_tag="PK522_05670"
     CDS             273..1307
                     /gene="queA"
                     /locus_tag="PK522_05670"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNC41254.1"
                     /translation="MKIQDFDFFLPAELIAQFPSERRTDSRLLCLDGPSGQYRDALFT
                     DLPNFLRPGDIVVLNNTQVIKARLFGTKETGGSIEVLVERILDEQHALVMIRASHAPK
                     IGSKLILADQISAIVNDRQQGFYLLQFEHALSVLQLLAQYGQLPLPPYIERDATLADE
                     DRYQTVFASEAGAIAAPTAGLHFDENLLRQLRELGVNIAYITLHVGSGTFQPVRTENV
                     LEHIMHSELYHIPEETTEAIQQAKRKGGRVLAIGSTTLRTLESCAQKHNGEIVPGHGS
                     TQLFITPGFKFQIVDRLLTNFHLPRSTLIMMISAFAGLENIRRAYCHAITQRYRFFSY
                     GDAMLIERKI"
     gene            1304..2389
                     /gene="tgt"
                     /locus_tag="PK522_05675"
     CDS             1304..2389
                     /gene="tgt"
                     /locus_tag="PK522_05675"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNC41255.1"
                     /translation="MKFQLLQTEGRARRGSLTLAHGTVETPVFMPVGTYGAVKTVSPA
                     ELQELQAQIILGNTFHLWLRPGLEVIEAHGGLHQFMQWHGPILTDSGGFQVFSLGQLR
                     KIQEQGVKFRSPINGDLCFLSPEESMRIQRILNSDIVMIFDECTPYLVDFAIVKASME
                     LSLRWAERSKRAHEGNSNALFGIVQGGMHENLREQSLQGLVQIGFDGYAIGGLSVGEP
                     KEDMQRILKHTAPLLPEDKPRYLMGVGTPADIVHAVAQGIDMFDCVLPTRNARNGWLY
                     TRNGIIKLRNSGYRLATQSPDEKCQCYTCKNFSLSYLHHLQKTNEALGARLNTLHNLY
                     YYQQLMREIRHAIETHQFEQYQREFMG"
     gene            2451..2915
                     /gene="yajC"
                     /locus_tag="PK522_05680"
     CDS             2451..2915
                     /gene="yajC"
                     /locus_tag="PK522_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNC41256.1"
                     /translation="MLISEAFAQATSGSQTEPGLMSLLPLIGILVIFYLLLIRPQSKR
                     AKEQIKMREALQKGDEVIISGGELGRVVSVGDNYVTLEIAPNVEIIVVKSGVQTLLPK
                     GTLKSIDTGKSSKSTKNNKTQKVTDTTESSNDDKNGEATDKETPSEGNKKES"
     gene            2976..4883
                     /gene="secD"
                     /locus_tag="PK522_05685"
     CDS             2976..4883
                     /gene="secD"
                     /locus_tag="PK522_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNC41257.1"
                     /translation="MNRYPLWKNLIIVISLFFGLLYTLPNFFGESPAIQISPLRIATA
                     TVNPELIKKVEDSLRKANIPTNGTIVEGSSIKVRFAETDTQIKAKDLLQAELGNDYIV
                     ALNLIPNSPDWLSSVGALPMYLGLDLRGGVHFMLQVDMVGALTKALDRYSADIRSSLR
                     EQKVSFSGIERERGQLTIKFRDEPARAKGETELKRIYPDLELRTTNMDRNFLLIATIK
                     PDAHARMQTAALQQNITTLRNRVNELGVAEPIIQQAGMDRVIVQLPGVQDTAKAKDIL
                     GRTATLEVRLVDDESDIDAALRGQAPLGTELFTERGGSPLLVKKQVLLTGDRITDAQP
                     GFDSDNQPAVHVNLDSNGARIFKDLTRNNVGKRMAILLVEKNLSEVITAPVIREEIGG
                     GRVQISGRMSTIEARDVALLLRAGALAAPMEIIEERTVGPSLGAENIARGFNSNLFGF
                     LAVAIFIMIYYRAFGVISVIALGANLLLLIALLSILQATLTLPGMAAIALTVGMAIDA
                     NVLINERIRDELRSGATPHMAISAGYERAFGTILDSNITTLIAGLALFSFGSGPIKGF
                     AVVLCLGIMTSIFSAVLVSRSLVNFLYGNQRKLENISIGTVWKSAVKTKRSGKNVKTK
                     NSELTNPIKDN"
     gene            4887..5819
                     /gene="secF"
                     /locus_tag="PK522_05690"
     CDS             4887..5819
                     /gene="secF"
                     /locus_tag="PK522_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNC41258.1"
                     /translation="MEFFRFERDIPFMSWGKYTTTISLLTFVLSVFFIAYKGLNLGVD
                     FTGGTVLEVNYAQPADLNKIRGTFSSIGISDVSVQNFGSSRDVMIRLPIKGDLSAAKL
                     SEIVIDALKKADDTVEVRRVEFVGPQVGQELLENGALALLFVSLGIVAYLAMRFEWKF
                     GVAGIIANLHDVIIILGFFAFFQWEFSLTVLAAVLAILGYSVNESVVIFDRIRENFRK
                     MRRAEVNQVINNAITRTMSRTIITHGSTQIVVLAMLFFGGEALFYFALALTIGILFGI
                     YSSVLVASPILLLLGVKREDLVTPEVKPQEEAVP"
     gene            5866..6471
                     /locus_tag="PK522_05695"
     CDS             5866..6471
                     /locus_tag="PK522_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNC41259.1"
                     /translation="MSEIINWIVETVGMLGYAGIVIMMFLESSFVPFPSEVVMIPAGY
                     LAFKGEMNLMIAILCGIVGSLSGALFNYYLAIKLGRPLLIRYGTYVMFNEDTLSRMEN
                     FFNRHGHISTFIGRLIPVIRQYISLPAGLANMNLVIFSLYTSLGAGIWVIILALLGYY
                     IGENEVLIKAYLHNIIVVLLLFCIIGATLYYWRYQKKQKRF"
     gene            complement(6479..6943)
                     /locus_tag="PK522_05700"
     CDS             complement(6479..6943)
                     /locus_tag="PK522_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41260.1"
                     /translation="MQASSDFQMNLNHFSVEIATESLRNLNLLQSSESTPPSLLNRMT
                     AQMQADAVFAGKLILAIQNLAVSEELDTNHQLIDKLNEAQHYINQFCDELGLRYKPGN
                     EHSASDNNNDDHSYSDAVSAADNLHSIIEILCSVIKTPSQSAEGIASKFFVV"
     gene            7142..8149
                     /locus_tag="PK522_05705"
     CDS             7142..8149
                     /locus_tag="PK522_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNC41261.1"
                     /translation="MDLLTQGLLGAALAQAGSKQQETRIATGVGFFAGLLADADILIQ
                     SDQDPLLTVEFHRHFTHSIFFVPFGALIAALMLWFFLRHKLSFLRLYFYCFLGYSLSG
                     VLDGLTSYGTHLFWPISQERVALNLISVIDPVFTLILLAGVIIAFRKKTKTFARISLF
                     LAGLYLCVGWVQLQRAENMTAFLAQTRGHVIETSIVKPTLANLILWRSIYEMNGKFYV
                     DAIRVGFFSEPKVYEGETIEKFIPGIHAPKLPEKSVLADDIERFTHFSAGYIGIQPER
                     PHLLIDVRYSNLPTSTDPLWAIVIDLHRPDQHAQYQLFRDASHEIRQKFLAFLLGEHI
                     N"
     gene            complement(8242..9993)
                     /locus_tag="PK522_05710"
     CDS             complement(8242..9993)
                     /locus_tag="PK522_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC41262.1"
                     /translation="MHSSPPEHANSSESLEKGNRQKWNAETSARGRILVMPRAGLPAK
                     ALAHILKEHNGKARKIGQSDLYIVDIPEYSEESVITALNQHPYLKYAEPDYIFYSSLT
                     PNDPYFGNAWHLSKTGATAAWDLSQGAGITIAILDTGVDSAHPDLAGKIVPGWNFYDN
                     NSNTVDVNGHGTAVAGAAAASSNNGVGVTAMAGQSKIMPVRIASPEATAYGSTIAQGL
                     TWAADQGARVANISYANVPASSTIINAAQYMKNKGGLVIVAAGNGGKDEGFTPTTSMI
                     PVSATNATDTKTSWSSFGNYVALSAPGIDIWTTYRNALYGTAWGTSLASPVVAGTVAL
                     MMAVNTKLGSTEIESLLFSTAKDLGSAGRDPYYGYGRVNAAAAVQAAKSNITATDTTP
                     PIVSTIEPLSGETVSGIVPVDIQAYDNVGVTRAELWVNNTSVAVDTSEPFAFAWDSAG
                     ASNGETNLVVRAYDAAGNMSLSNAISVTVSNPIQPPIVDTEAPIISIINPLTGNVSGA
                     VTITANASDNNGASGITLSIYIDGVLKTTGIGSALSMSWNTRSKYVKAGMHTIKAIAK
                     DAAGNSSAKSVSVNVIK"
     regulatory      complement(10278..10364)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(10542..11597)
                     /locus_tag="PK522_05715"
     CDS             complement(10542..11597)
                     /locus_tag="PK522_05715"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNC41263.1"
                     /translation="MRQRVRNLSATLFLILILGTFYGTVPYYQNYFSSSTDFLGQEYS
                     HWQVLLTAMLAYVVILSIFYLTEKSPRISKSIYCLRAIQRLMRSPKSILQTGLPFEER
                     LGLLSILLKGFFIPLMVVWLFAHTGHMIEHGNKLAVALNQATIEWISLFNAHGFWFMF
                     KVILFLDVLFFTVGYMIELPSLKNEIRSVDPTLMGWFVALASYPPFNGLTSSVFAGYT
                     TDFPQFNDPILHIALNIFLLILMAFYTWASIALNLKASNLTHRGIIMHGPYRYIRHPA
                     YTCKNLAWWIGVLPAVIAATETSITATLLAVGSMLGWSIIYILRALTEEDHLRSVDNE
                     YDLYCQKVKYRFFPGII"
     gene            complement(11761..12555)
                     /locus_tag="PK522_05720"
     CDS             complement(11761..12555)
                     /locus_tag="PK522_05720"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNC41264.1"
                     /translation="MNKSFKNKVAGVASAVAIAMSASAAYAYVETQNFWGTTSQSGGI
                     VTFSNEGVGANQLKITFDNTSNFSGIITGIVFNITQNINNASVLSFVDGNGTNLASLW
                     NIGFNVNNNITPGNTVFDIAFTTNNGIIGGIYNSGVATSTANTYPDIATLVLQISDPN
                     PWNFSDVGDDSILRTQRTGANGNGSDKIVTSSSSTSTSGGTSTSSTSGGTSTSSTSGG
                     TTTSSTSGGTSNGNTVSEPGVLALLGIGLLGQALMFVARRRRLGSK"
BASE COUNT         3572 a         2435 c         2790 g         3832 t
ORIGIN      
        1 atggcgcgcc cggcaggatt cgaacccacg accccttggt tcgtagccaa gtactctatc
       61 caactgagct acgggcgcaa ttataatcga gttgcagtat tgttaaccac gattgcacgt
      121 attatatcaa acctatttca ctaagcgcga gtgcaatcga tttaagttac ctcatttttt
      181 ttcaaacaaa gatctctcaa agcaagcacg caatcctgtt acattcgctg acatttgtca
      241 aggagtagcg taggaacagt ataataagcg ggatgaaaat acaggatttt gatttttttt
      301 taccggctga attaatcgcg caatttccat ctgagcgccg cactgacagc cgtttgcttt
      361 gccttgatgg tccttcgggc caatatcgcg atgcgctgtt tactgatttg cccaattttt
      421 tgcggcctgg tgatattgtg gttcttaata atacccaggt catcaaagca agactttttg
      481 gaactaaaga aacaggtggt agcattgaag tacttgttga gagaatactc gatgaacagc
      541 atgcactcgt gatgatacga gcgagtcatg cgcctaagat tggttcaaag ttaatcttgg
      601 ctgatcaaat cagtgcgatc gttaatgacc gtcagcaagg cttctattta ctccagttcg
      661 agcacgcact ttcagtattg cagttgctag cgcaatacgg acaattgccg cttccccctt
      721 acattgaacg cgatgcgacc ttagcggatg aagatcgata tcaaaccgtt tttgcaagtg
      781 aggcaggtgc aattgcggca cccactgctg gactgcactt cgatgaaaat ttgcttaggc
      841 aattacgtga attaggcgtg aatattgctt atattacact ccatgtgggt tcagggacat
      901 ttcaacctgt tcgaactgag aatgttctgg agcatattat gcacagcgag ctgtaccata
      961 ttcccgaaga aaccacggaa gccattcagc aagcaaagcg caaggggggg cgagttttag
     1021 cgatcggtag cacaacattg agaacacttg agtcctgtgc gcaaaagcac aatggagaaa
     1081 ttgttccagg tcacggcagc actcagcttt ttatcacacc tggttttaaa ttccagatcg
     1141 ttgatcgttt gctgactaat tttcatctac cacgttcaac gctaatcatg atgatttctg
     1201 catttgcagg tcttgagaac attcgtcgtg cttattgcca tgcgattaca caacggtatc
     1261 gtttttttag ttatggtgat gccatgttaa tagaaaggaa aatatgaagt ttcagttgct
     1321 tcaaaccgag ggtagggcaa ggcgaggatc gttgacactt gcgcatggca cagtagaaac
     1381 gcctgttttt atgccagttg gcacttacgg tgcagtcaaa acggtttcac cggctgaact
     1441 tcaggaactc caagcgcaaa ttatattagg aaataccttc catttatggt tacgtcctgg
     1501 tctcgaagtc attgaagcgc atggtgggct acatcaattt atgcaatggc atggtccgat
     1561 attgacagat tcgggtgggt ttcaagtttt tagtctgggt cagctacgca aaattcagga
     1621 acagggcgtc aagtttcggt ctcccattaa cggcgatctt tgttttcttt ctccagaaga
     1681 atcgatgcga attcagcgca tactgaattc tgatattgtg atgatttttg acgaatgcac
     1741 gccttatttg gttgattttg caatcgtaaa ggcttcgatg gaattgagtt tgagatgggc
     1801 ggaacgttct aagcgtgcgc atgaaggaaa ctccaacgca ctatttggaa tcgttcaagg
     1861 cggtatgcat gagaatcttc gtgagcagtc cctgcaagga ttggtgcaaa ttggctttga
     1921 tggctatgcc attggtggtc tgtcagtggg cgaacccaaa gaagatatgc agcgcatttt
     1981 gaaacacact gcccctttat taccggaaga taagccgcgt tacttgatgg gggtgggtac
     2041 gccagcagat atcgtgcatg ctgtagcgca aggcattgat atgtttgatt gcgtgctgcc
     2101 aacacgcaat gcacgtaacg ggtggcttta tacaagaaac ggtattatca agctgcgtaa
     2161 tagcggttat cgccttgcaa cgcaatcacc agacgagaag tgccaatgtt atacctgtaa
     2221 aaacttctca ttgtcttatt tgcatcattt acaaaaaacc aatgaagcac taggggcacg
     2281 gctcaataca ctacataatc tatattacta tcaacagctt atgcgtgaga tccgacacgc
     2341 aatcgagacc catcaatttg agcaatatca gcgagaattt atgggctagc ctaaggccgt
     2401 gctaaaatgc ttagatttta aatttaaaca attatagagg agatacgcga atgttgatta
     2461 gtgaagcatt tgctcaggcg acgagtggaa gtcagactga gcctggatta atgagtttgt
     2521 tgcctttaat cggtatttta gtcattttct acttactttt aattcgccct caatccaaac
     2581 gtgcaaaaga gcaaattaag atgagagaag ccttgcagaa gggtgacgaa gtcatcatca
     2641 gcggaggtga gttggggcgc gtcgtgagtg ttggtgataa ttatgtcaca ctcgaaattg
     2701 cgcctaatgt ggaaattatc gtcgttaaat ccggtgtgca aactttgttg ccaaaaggaa
     2761 cactgaaaag cattgatacc ggtaaaagta gtaaatcaac taaaaataat aaaacacaga
     2821 aagttactga cacaactgaa agtagcaatg atgataagaa tggcgaagca actgataaag
     2881 aaacgccttc agagggtaat aaaaaagaaa gttaagcatt ttttatacgc gttctgttca
     2941 aatcaaattc tgtgtaccaa aactaattaa ttaatatgaa tcgatatccg ctctggaaaa
     3001 atctgattat tgtcatttcc ctcttctttg ggttgctgta taccttacct aatttttttg
     3061 gggaatctcc ggcaattcaa atttcgccgc ttcgcattgc aaccgcaacc gttaaccctg
     3121 aattgatcaa aaaagtcgaa gatagcttac gtaaagcaaa tattccaacg aatggaacga
     3181 ttgtggaggg aagtagcatc aaagttcgct ttgctgaaac ggatacacaa atcaaagcca
     3241 aggatctttt acaagcggaa ttgggaaacg actatatcgt tgcattgaat ttgattccaa
     3301 attcacctga ttggctgagt agtgtcggtg ctttaccgat gtatcttgga ctcgatctcc
     3361 gtggaggggt ccacttcatg ttgcaagtgg atatggtagg cgcgttaact aaagcgcttg
     3421 atcgatacag cgcggatatt cgtagtagtt tacgggagca gaaagtttcc ttctcaggaa
     3481 tcgagcgtga aagaggacaa cttacaatca agtttcgcga tgagccagca cgtgccaaag
     3541 gtgaaactga gcttaaaaga atctatcctg atttggaatt gcgaacgacc aatatggatc
     3601 gaaacttttt actgattgca accattaaac ctgatgcaca tgcgcgaatg caaaccgcag
     3661 ctttacagca gaatattacg acattgcgta accgcgttaa tgagcttggt gtggctgagc
     3721 ctattattca gcaagcagga atggaccgtg tcatcgttca attgcctggc gtacaagata
     3781 ccgccaaggc gaaagatatt ttaggaagaa cagcgacgct cgaagtccgg ttagtggatg
     3841 acgaaagtga tatcgatgca gcattacgcg gacaggctcc tttgggtacc gagcttttta
     3901 ctgagcgagg cggcagtcct ctgctggtta aaaaacaagt attgctgaca ggagatcgca
     3961 ttaccgatgc gcagcctgga tttgatagcg ataatcaacc tgctgttcat gtaaatctcg
     4021 atagtaatgg ggccagaatt tttaaggatc tgactcgtaa taacgtaggt aagcgcatgg
     4081 ctattttgct ggttgaaaaa aatctgagtg aggtcattac tgcgccggtg attcgtgaag
     4141 agattggggg agggcgggtg caaatcagtg gccgaatgag tacgattgaa gcccgtgatg
     4201 tggccttact gctgagagcc ggcgcgcttg ccgcaccgat ggaaatcatc gaggaacgga
     4261 ctgtagggcc tagcttgggt gcagaaaata tcgcaagggg gttcaattca aacttgtttg
     4321 gattcctagc cgttgcaatt ttcattatga tttactatcg agcattcggt gtcatttcag
     4381 tcattgcgct cggtgctaac ttattgctat taatagcatt actttcaatt ttacaagcaa
     4441 ccctgacatt gccgggtatg gcagccattg cgttaacagt cggtatggcc attgatgcaa
     4501 acgttctgat taatgaacgt attcgagatg aattgcgaag tggcgctact ccccatatgg
     4561 ctattagtgc cggttatgag cgagcctttg gaacaatttt ggactctaat atcactacat
     4621 tgattgcggg acttgcgtta ttttcgtttg gatcgggtcc tattaaagga tttgccgtgg
     4681 tgttgtgctt aggaatcatg acttcaattt ttagcgcggt gttggtttca cgttctttag
     4741 ttaatttttt gtatggtaat cagcgcaaac ttgaaaatat ttcgatcgga acagtttgga
     4801 aatctgcggt caagactaaa cgttctggca agaatgtaaa gactaagaat agtgaattaa
     4861 caaatcctat taaggataat taacctatgg aattttttag atttgaacgc gatattccct
     4921 tcatgagctg gggaaaatat actacgacca tttctttgtt gacgtttgtt ttatccgttt
     4981 tttttattgc ttataaagga cttaatttag gtgttgattt taccggtggt acggttctgg
     5041 aagtaaatta cgcacaaccg gcagatctga ataaaattcg tggcaccttt agttcgatcg
     5101 gtatctcgga tgtgtcggtc cagaattttg gatcttcacg cgatgtcatg attcggttac
     5161 cgattaaagg tgacctatcc gctgcaaaat tgtcagaaat tgttattgat gcgctgaaga
     5221 aagctgatga taccgttgaa gttcgccgtg tagagtttgt gggaccccaa gttgggcaag
     5281 aattactgga aaacggtgcg ctagcacttc tttttgtatc gttgggtatt gttgcgtatt
     5341 tagcgatgcg ctttgaatgg aaatttgggg tagcagggat tattgctaac cttcatgatg
     5401 tgattatcat cctggggttt tttgcttttt ttcaatggga attttcttta acggttctcg
     5461 cagccgtgtt ggcaattctt ggttattctg tcaatgaatc agtcgtaatt tttgaccgga
     5521 ttcgtgagaa tttccggaaa atgcgaagag ctgaggtcaa tcaagtgatc aataatgcta
     5581 ttacgcgaac gatgtcccgt accattatta cgcatggaag tacacagatt gtcgtattag
     5641 ctatgttgtt ttttggtggc gaggctttgt tctattttgc actggcttta acgattggta
     5701 tcttatttgg tatttactcc tcagtcttag tcgcaagtcc tatcttgtta ttgttagggg
     5761 ttaaacgtga ggatttggtg acacccgaag tgaaaccaca ggaagaagct gttccctaag
     5821 gtattttcta attacctttc gtttaattga aagagagtgc acagcttgtc cgaaataatc
     5881 aattggattg ttgaaaccgt tggtatgctc ggttatgcag gaattgtgat tatgatgttc
     5941 ctggaatcaa gctttgtccc ttttcccagt gaagtggtga tgattccagc tggttatcta
     6001 gccttcaaag gagagatgaa cctaatgatt gcaatcttat gtggaatcgt tggtagtctt
     6061 agcggcgctt tatttaatta ttacttggcg atcaaactag gtcgcccact cttgattcga
     6121 tatggtactt atgtcatgtt taacgaagat actttgtcga ggatggaaaa tttctttaat
     6181 cgccatggcc atatctcgac cttcattggg cgtcttatcc ctgtcattcg gcaatatatt
     6241 tctttgccag cagggttagc taatatgaat ttagtgatct ttagccttta cacaagttta
     6301 ggggctggaa tctgggtcat cattttagcg ttgttaggct actatattgg ggagaacgag
     6361 gtattaatca aagcatacct acataatatt attgtagtgc ttttactgtt ttgtataatt
     6421 ggtgctacgc tttattattg gcgttatcag aaaaagcaga agcgtttcta gtgctcgatt
     6481 aaacaacaaa aaacttagaa gcaatacctt ctgcagattg ggatggtgtt ttgattacag
     6541 aacatagtat ctcaataatg ctatgcaaat tatctgctgc tgaaactgca tcggaataac
     6601 tatgatcatc gttattattg tctgaggctg agtgttcatt tcctggttta tatctcagac
     6661 ctaattcgtc acaaaattgg ttgatatagt gttgcgcttc gttaagttta tcaataagtt
     6721 gatggttggt atcgagttct tctgatacag ccaaattctg tatagctagg attaattttc
     6781 cagcaaatac agcatctgct tgcatctggg cagtcattcg attaaggagt gaaggggggg
     6841 tagattcaga tgattgcaat agattaagat tgcgtaagct ttctgtcgca atttctacgc
     6901 taaaatgatt cagattcatt tgaaagtcac tggatgcttg cattttggat tcccttaaca
     6961 aggtatatca aattaatatc gctaaatcat gtcctatctt taggaatgat cagcgataac
     7021 aatccttcta tttcattagg attgcattac aatttgagac aatacctaat tatatgattt
     7081 ttaatgatta gaacaaataa attattcagc atcgcgtgaa taagcataat aatatagtga
     7141 tatggattta ctgacacagg gtttattagg tgcggcttta gcgcaggcag gttcaaagca
     7201 gcaagaaacg cgaattgcaa cgggagttgg tttttttgcg ggcttattag cagatgctga
     7261 tattttgatt caatctgatc aagacccttt gctaaccgta gagtttcatc ggcatttcac
     7321 tcattccatt ttttttgttc cattcggtgc gttaatcgct gcactgatgc tttggttttt
     7381 tttgcgacat aagctatcgt tcttgcgact gtatttttac tgttttctag gttatagctt
     7441 aagcggtgta ttagatggct taacaagcta tggcacgcat ttgttctggc caatcagtca
     7501 agaacgtgtc gcgttaaatc tcatttcagt gatagaccct gtttttacgc tgattttact
     7561 ggcaggtgtc attatcgctt ttagaaaaaa aacaaaaaca ttcgcacgca taagtttatt
     7621 ccttgctgga ttatatcttt gtgtgggttg ggttcagcta caacgcgcag aaaatatgac
     7681 tgctttcctc gcacaaacac gaggtcatgt tatcgaaacg agcatcgtga agcctacact
     7741 tgcaaattta atactatggc gatcaattta tgaaatgaat gggaagtttt atgttgatgc
     7801 gatacgcgtt ggtttttttt cagaaccgaa agtatacgaa ggtgaaacca ttgagaaatt
     7861 tattcctggt atacatgctc caaaattacc tgagaaatca gtactagcag acgatattga
     7921 gcgatttacc catttttcag ccgggtatat tggcattcaa ccagaacgcc ctcatttact
     7981 gattgatgtg cgctattcga atttaccaac aagcactgac ccgttatggg cgattgtgat
     8041 tgatttgcat cgaccggatc agcatgcgca ataccaatta ttccgagatg catcgcatga
     8101 gattaggcaa aagtttttag catttctact tggggaacat attaattaga aaagtattag
     8161 gcgagtggga ttaatactag cgtaagtagc gagaacacgt gcttttaacg gcaagcacgt
     8221 gtttggttca tcagtaggac attactttat aacattaaca ctgactgatt ttgctgatga
     8281 attacccgca gcatcttttg ctattgcttt tatcgtgtgc atgccagcct taacatactt
     8341 agaacgtgta ttccaactca tacttagtgc acttcctatc cccgttgttt ttaggacacc
     8401 atcaatataa attgaaagag tgataccgct tgcaccatta ttatcggatg catttgccgt
     8461 aatggtgact gctccagata cattacctgt tagtggatta ataatgctaa tgataggtgc
     8521 ttcagtatcc acgataggcg gttgaatggg attactaact gtaacgctga tcgcattgga
     8581 tagcgacata ttgcctgcgg catcataagc acgaacaacc aaattcgttt ctccatttga
     8641 tgcaccagca gagtcccagg caaatgcaaa tggttcagaa gtatcaacgg caacactcgt
     8701 attgttgacc caaagctcag ctcgtgttac accaacatta tcataagctt gaatatcaac
     8761 gggaacgatt ccacttacag tttccccgga aagtggttca atagtcgata cgattggtgg
     8821 cgttgtgtcg gttgctgtta tatttgactt agctgcctga actgcggcag cagcatttac
     8881 acggccatat ccataatagg ggtcacgacc tgccgagccg agatcttttg ctgttgaaaa
     8941 caataagctt tcaatctctg tactgcccag ctttgtgttg actgccatca tcaatgctac
     9001 agtacctgct acgactggac ttgccagtga agttccccaa gctgttccgt ataatgcatt
     9061 gcggtaggta gtccagatat caatacctgg agctgataat gcgacataat taccaaaact
     9121 agaccaactg gttttagtat cagttgcatt ggtagctgat actgggatca ttgatgtggt
     9181 tggcgtaaaa ccttcgtctt ttccaccatt acctgcagca acaataacta gccccccttt
     9241 atttttcata tattgtgcag cattaatgat tgtggaactt gctggtacat tcgcatagct
     9301 aatgtttgcc acacgtgctc cttgatcggc tgcccaagtt aggccttgtg cgatagtact
     9361 accataagct gttgcttcag ggctagcgat tcttacaggc atgatttttg attgcccagc
     9421 catcgctgta acaccaactc cattattgct ggaggctgct gctgcaccag caaccgcagt
     9481 gccatgccca tttacatcga cagtattgga gttattatca tagaaattcc agccagggac
     9541 tatttttcct gctagatctg gatgtgcgct gtcaacgcca gtatcgagaa tggcgatagt
     9601 gatgcctgcc ccttgtgaaa gatcccaggc agcagtagct ccagttttgg aaaggtgcca
     9661 tgcgttgcca aagtatgggt cattgggagt aaggctagag tagaaaatat aatcaggctc
     9721 tgcatatttc aaatacggat gctgatttaa agcagtaatg acactttcct cagaatattc
     9781 cggtatatca acaatatata aatcactctg gcctattttt ctggctttac cattatgctc
     9841 tttcagaata tgagccagag ccttagcagg caaccctgct cgaggcatta ctaaaatcct
     9901 acctcttgct gatgtttctg cattccattt ttgacgattt cctttctcaa gtgattcgct
     9961 tgaatttgca tgttctgggg gagatgaatg caattgggtg gggatcagaa gaaatgatac
    10021 aaataagcat acaaaaacag aaaaactaag aaagttgtaa atttgatttt ttgacgcact
    10081 gaaaaataaa taactttgat tggtgctttc catgaattta tcctttactt ttttaaattc
    10141 aggggaagcc cgggaaagga actgcatgag tcgttgcgtt gcatggcttg ctagttttat
    10201 ataaaaagcg agttgcagcg cttgaactga tcttcgaatt caaacaattg cgagtgtttg
    10261 cttaatgaag aatactatct ggcagtcttg ctatcctggg gaattttccc tgtagatcaa
    10321 tgactttgcg tccctcactt tcgcgaggtt tgcctttaac aaatagttgt ttgctgtaag
    10381 tagtactcac agacaaaaaa actatcgaat atttatcgga tagattggaa gaatagtaaa
    10441 gaagtcactt gctgataaac gagtccagcg ttgtgagatt tggtttttgt tgagaatgaa
    10501 atacaaagta agtggagtag cgctactaat gatttgattt gctagataat accggggaag
    10561 aatcggtatt taactttctg acaatataga tcgtattcgt tatctacact gcgcaaatga
    10621 tcttcttccg ttaatgcgcg taggatataa atgattgacc aacccagcat agatccaacg
    10681 gcaagcaaag ttgctgttat ggaagtttct gttgcagcga ttactgcggg taatacgcca
    10741 atccaccaag ctaaattttt acacgtgtaa gctggatgac gaatatagcg ataagggccg
    10801 tgcataataa ttcctcgatg cgtcaaatta cttgccttga gattaagtgc aatcgaagcc
    10861 caagtataga aagccattaa gattaataaa aaaatattta aagcgatatg taaaatcgga
    10921 tcattaaatt gaggaaaatc cgtggtatat ccagcaaaaa cagaagaagt tagaccgttg
    10981 aaaggggggt agctggcaag tgctacaaac catcccatta aagtagggtc gacagatcgg
    11041 atttcatttt taagtgatgg caactcgatc atgtagccaa ccgtaaagaa cagaacatct
    11101 aaaaagagaa ttactttgaa catgaaccaa aagccatgtg cattaaataa cgatatccat
    11161 tcgatggttg cttgatttaa tgctacggct aatttatttc catgctcgat catatgtcct
    11221 gtgtgagcaa ataaccaaac gaccatgagc gggataaaga accctttaag taggatactc
    11281 agtaatccaa gtctttcttc aaaaggtagt cctgtctgca aaatgctttt gggtgacctc
    11341 atcagacgtt ggattgcacg taggcagtaa attgatttag aaattcttgg tgatttttcc
    11401 gtcaaataaa aaatagagag aataacaaca taagctaaca ttgccgtcag cagaacttgc
    11461 cagtgactat attcttggcc aagaaagtcg gttgatgaag aaaagtagtt ttgataataa
    11521 ggtacggttc cgtaaaaagt acctaaaatc aatattaaaa atagagttgc tgataaattt
    11581 ctgacgcgtt gtctcattga cttctaatag gtaattaaag ctcaaacaaa atagcattaa
    11641 tttaattcgt gcaatacaaa aagaaaatgc gcctaagcgc attttcttta aacctttcat
    11701 ttaaatgcta caaactattt catagtaatt aaaattaagc taactataag ttgttagcgt
    11761 ttattttgat cctaggcgac ggcgtcttgc tacaaacatc agtgcttgcc cgagtaaacc
    11821 aatgcctaat aatgctaaaa cacctggttc tgaaacagta ttgccattgc ttgtgccacc
    11881 actggtacta ctcgtggttg taccaccact cgtgctactg gttgaggttc caccgctggt
    11941 actgcttgtt gatgtaccac cactggtgct cgtgctacta ctgctggtaa caattttatc
    12001 gcttccgtta ccatttgcgc cggtacgctg tgtccgcaat atagagtcat ccccaacatc
    12061 actaaagttc catggattcg gatcggagat ttgtaatact aatgttgcga tatcgggata
    12121 tgtgttagcc gtgctggttg caactccaga attgtaaata ccgccaataa tgccattatt
    12181 cgtcgtgaaa gcgatgtcaa acacagtatt acccggggtg atattgttat ttacattaaa
    12241 accaatattc cagaggctgg ccagattagt accattgcca tccacaaatg ataaaactga
    12301 tgcgttatta atattttgag taatattaaa aacgatcccg gtgatgattc cactgaaatt
    12361 tgaagtgtta tcaaaagtaa ttttgagttg atttgcgcca acaccttcat tgctaaatgt
    12421 aacaatacca cccgactgag aagtggtacc ccaaaaattc tgagtttcaa cataagcgta
    12481 ggctgcactt gcgctcatcg caatagctac tgcgcttgcg acacccgcta ctttgttttt
    12541 aaaagattta ttcattgatt gcttccctca ttaatctaaa tgcctatgaa ctattctcag
    12601 tgtttttcat cttatatagc aagatcaat
//
LOCUS       DAOQLJ010000059        12626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1897_length_12626_cov_6.071673, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000059 DAOQLJ010000000
VERSION     DAOQLJ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12626
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1897_length_12626_cov_6.071673"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1030
                     /gene="dapF"
                     /locus_tag="PK522_05725"
     CDS             191..1030
                     /gene="dapF"
                     /locus_tag="PK522_05725"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNC41265.1"
                     /translation="MKLKFTKMQGLGNDFVVIDAINQKITLTATDIRFLADRHFGIGC
                     DQVLLVETSQNDADFRYRIFNADGNEVEQCGNGARCFVRFVHDHALTVKNEIRVETAS
                     GTIIPKLEESGEVTVNMGIPRFDPADIPFTAEKRALTYSLEINHKQIEISAVSMGNPH
                     AVQIVPNLEQAPVLTEGPLIESHARFPKRVNVGYMQIIDRQSIKLRVFERGAGETLAC
                     GTGACAAVVAGIQRGLLNSNVEVNTQGGKLKISWVGENQPVWMTGPATTVFEGIIVLP
                     VKF"
     gene            1069..3435
                     /locus_tag="PK522_05730"
     CDS             1069..3435
                     /locus_tag="PK522_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41266.1"
                     /translation="MNIAHKWRFFRSGGFDQVRLETGADIKFLRTLDPKLWAALSCPT
                     NNLEFDSKTLEFIDTDGDGHIRVPEIIAAVEWAASVLKNPEDLTQGADALQLNAIDDS
                     TPEGAALLASAKQILLNIGKKDEATITVEDTADLNKIFASTKFNGDGIIPATAAGDTE
                     TQIVIEDIMKCVGSVQDRSGLPGISAEHIEQFFTEAKAYSEWWQDAERDAANVLPLGE
                     NTEAAKMAFDAVKTKIDDYFTRCKLAEFDQKASDPLNPSLLNYEALTNTNLSSTTEQL
                     SALPIARIEAKKPLPLNEGINPAWADAISSLKKQVIQPLLADKEQLSIDEWQALCDKF
                     AAHQAWLNIKQGAAVEVLGIKRIRSILAGSYQDKVSALLQKDQSLASASDAINSVEKL
                     IRYHRNLFQLLNNFVSFRDFYTAQNKAIFQVGSLYLDGRSCDFCLRVSDINKHSSMAN
                     LSGTYLAYCECQRRGGSEKMYIAAAFTNGDADNLMVGRNGIFYDRKGQDWDATIVKII
                     EHPISVRQAFWYPYKRIGKMIGEQIEKMASAREKSVQDQAASGIADASQKAEAGKPPT
                     PPFDVGKFAGIFAAIGLAIGAIGTAIASVLTGFLSLVWWQMPLAILGLILLISGPSVL
                     IAFLKLRKRNLAPLLDGNGWAVNTRAIINIPFGTSLTHMATLPVGAQRSLVDPYAEKK
                     SPWKTYLFILVLLGSIAYLLHSGHLNQGTIDELRKRFSSNKAEVTQEASGTQEISPTP
                     PQTETLPDNSRIETDNTPQSAPTESKSESAAPSGPASIAPAPKPSVAP"
     gene            3584..5278
                     /locus_tag="PK522_05735"
     CDS             3584..5278
                     /locus_tag="PK522_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC41267.1"
                     /translation="MRTLITYIMVFPRRSTFVLVALLFAGVAEALSLTALLPLISIAV
                     GETDDSKIGRFMVQALERIGIAPTIDAILLIIVGGMLIKGVILLLTNRQVGYTVAHVA
                     TALRLDLIEALLASRWQYYLRQPVGSLANSVATEAYRAANGFEHSVNVLALAIQVIVH
                     AIVALFISWQATFGSLLIGIVLFIALHRLVRATRRAGTKQTHLLRHLLSYLSDVLSSV
                     KSLKAMARDNVADAILHEQSIQLEKATRKEVISRAALLALQEPILAALTASGLYIALV
                     VWNLSLPEVMVMVFLLTRILGLLNKTQRRYQQLTVQESAYWALRTAAEDARAAAERAT
                     GTITPTLQQGISLRHAAFDYDRKPIFQDLNIEIPIKSFTAVIGSSGVGKSTLLDLLCG
                     LAEPKSGEILIDGVPLHNIDLRQWRHLIGYVSQDTVLLHDTILNNTLVGAPALTIEDA
                     ERALKQAGAWDFISSFPEGIQTVVGERGGMLSGGQRQRIAIARALAHQPLLLLLDEPT
                     SALDPQSEQLICETLKKLAEDYTIVAVSHQPAVINAADRVFILSNGQADLLTNTPK"
     gene            complement(5280..6509)
                     /locus_tag="PK522_05740"
     CDS             complement(5280..6509)
                     /locus_tag="PK522_05740"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HNC41268.1"
                     /translation="MEVFLVGGSVRDELLGLPVKDHDYVVVGSNPEKMVQNGFRPVGK
                     DFPVFLHPVTHEQYALARTERKVSRGYKGFEVYASPDVTLQEDLARRDLTINAIAKDQ
                     TGKIIDPFNGIADLEAGILRHISPAFVEDPVRILRVARFAARFGFHIAPETMELMKEM
                     VHTGEVDALVPERLWQEIARGLMEKRPSQMFYALRACNALSRVLPEVDVLFGVPQPAQ
                     HHPEIDTGVHIMIAIDYAAQNNYSLAVRFATLTHDLGKGTTPPSEWPRHVGHETRSVT
                     LVKDLCERIRIPKEERDLALIVARYHGDVHRAEELRPATIADILQATDAYRKPDRFAA
                     FLQACACDFHGRPGYAEKPYAQSVRLNKAFDAARSVDAGVIAANLKQKDIDLLDLPSM
                     INRSIREARIAAIVNSQ"
     gene            complement(6525..8438)
                     /locus_tag="PK522_05745"
     CDS             complement(6525..8438)
                     /locus_tag="PK522_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNC41269.1"
                     /translation="MRKIIFCLMLVVSTIVTAASLEDDFLKLREAFRVGDAAKLALYA
                     PRFQRHALAPYAEYYQIRLYLETTSVDSIQAFLQRHQGSLIGDKLRGDWLRVLGKRQQ
                     WTLFAEEYPKLINKDEGLICYALQLRLASKDKEVIEEARSLWFTEKDMPDSCTPVFSQ
                     LIANQKLSHEDIWFRIRLALEAGNTGVARQVSRYLPGNHELNAQKLNEASKNPLRYLE
                     KFQGSYSNRADREIVLFALGRLLRSEINQAYVHLLKIRNHFPPTDQSFFMSSLAYRAA
                     LKQDDRALDWFIQAVDKHPYPLSEAKHAWMGRVALRKGNWNHVLQIIDNMPDSMQKMD
                     VWRYWKARALLKNGELGEANAILAPLSNEHSYYGQMAKEELGVMMSSPAANYHPSSQE
                     IHAMQQNPGIQRALALYQFNQRIEAAREWVWTIRNFTDEQLIAAAEVAFRHGIYDRAI
                     NTAIQTVSQHNFNLRYLAPYRDQMREVLKHQELDEAFVYGLIRQESRFLADVKSTAGA
                     MGLMQLMPATAKWVAGKLGMQQFQNHLVTDINTNLRLGTYYLKHVLSLFDNQPLLAAA
                     AYNAGPGRAKQWRDPEKPLEGAIYAETIPFNETRDYVKVVLNNSVYYANNFGHQNIPP
                     LRQRLGVVAPKPQ"
     gene            complement(8541..9884)
                     /gene="gdhA"
                     /locus_tag="PK522_05750"
     CDS             complement(8541..9884)
                     /gene="gdhA"
                     /locus_tag="PK522_05750"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNC41270.1"
                     /translation="MKYKGIEEFRNYVSERNPGQPEYMQAVTEVIESLWPFITNNPRY
                     AEHGLLDRIVEPERIIIFRVAWVDDHGEVKVNRGYRIQHSSAIGPYKGGIRFHPSVNL
                     SILKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGRSPGEVMRFCQAFVSELFRHVGSD
                     TDVPAGDIGVGGREVGFMAGMVKKLTNRADCVFTGKGISFGGSLVRPEATGYGTVYFV
                     EEMLKHVGQSLNGLRVAVSGSGNVAQFAVEKAMAFGAKVITVSDSSGTIVDEAGFTPE
                     KLAILAEVKNHLYGRVSDYAERVGAKFIPGERPWRVPVDVALPCATQNEVSEDDAATL
                     IKNGVICVAEGANMPCTAGAVKRFEHAKVLFAPGKASNAGGVATSGLEMSQQAMRLFW
                     SHEEVDARLQEIMRAIHNSCLRYGEQSDGRINYVDGANIAGFVKVADAMLAQGVI"
     gene            complement(10219..11463)
                     /locus_tag="PK522_05755"
     CDS             complement(10219..11463)
                     /locus_tag="PK522_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41271.1"
                     /translation="MRLVFKDFFRYCSTIIIVFNLSWIISACQTFDQSSQQSRFIENP
                     AAIGSQFPYLSSLPDGSMLMSWIEGKGNHQVLKFAIYRQDRWVRIGQAVQGSGWYVNW
                     ADYPAVVAIDDSFWVAHWRVKSKTEQIYDYDVFFSISVDAGVTWSAPQKPYQDDTAAA
                     HGFVSVFPALGQAGIVWLDGRDVAKKISDRFSLRYALIHRNGQIDSDRVIDDNTCTCC
                     WTASAVTSKGPVVVWRSRRGDEIRDHHFAQLIDGIWSDPQPVSQEGWSIAGCPVNGPS
                     IATNGKYVAASWFTAEGNRPRVRSAFANDSSLQFNQALDIDDEKPIGRTRVIALTDET
                     AIVIWITAMDKVTRKASVAARSIGMNGNMGQIKRLSEINPGRDSGVPQIVKNRSSVII
                     AWTQTEPPSGVKLLFVPLEIFD"
     gene            complement(11618..11914)
                     /locus_tag="PK522_05760"
     CDS             complement(11618..11914)
                     /locus_tag="PK522_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HNC41272.1"
                     /translation="MNWYRRENNGKLILSLYIQPGTKNTQFAGLHGDALKVSLAAPPI
                     EGKANKALIKFLADCFQVSANQVSIKRGAHSRYKLVEIEQATIDPGLLLEGESS"
     gene            complement(11914..12486)
                     /locus_tag="PK522_05765"
     CDS             complement(11914..12486)
                     /locus_tag="PK522_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNC41273.1"
                     /translation="MSSQILIFLLETILGLFSLACLLRFYLQLFRVHYDNPLSKFLIA
                     VTNFIVLPTRRVIPSWKGFDLSTFFLAWIGQFLIMVSTSFIQGYELETVSGNTMIGFG
                     LLSLLELVRLTLYIVMAMVIVQAILSWINPYSPIAPLLTSFTRPILGIFQRFIPPIAN
                     VDLSPLFVLVAVQLLLMVLTGLQQEIAVLF"
BASE COUNT         3647 a         2984 c         2543 g         3452 t
ORIGIN      
        1 tttctgaaga catattgagc tcctatttct atttcctaat taacccctat aattagcaaa
       61 atttgcaggg ataacagaaa aagcaggtta ttgcaagcat caggttcgag ctttatttaa
      121 tagatcaggc aataagccat tgaaattatt aggcacacat gcgctaaccg ctttccatca
      181 caagcaagct atgaagttaa aattcactaa aatgcaggga ctaggaaatg actttgttgt
      241 cattgacgct atcaatcaga aaattacact caccgccacc gatattcgat ttttggctga
      301 ccggcatttt ggtattggtt gcgatcaagt tctattagtt gaaacatctc aaaatgatgc
      361 tgacttccgt tatcgcatat tcaacgcaga tggaaatgaa gttgagcaat gtggaaatgg
      421 ggcacgctgc tttgttcgct ttgttcacga ccatgcattg acggttaaaa atgaaattcg
      481 tgttgaaacc gcaagtggta ctatcatacc gaaactagaa gaaagtggtg aagtcactgt
      541 caacatgggt atacctcgct ttgatcctgc tgatattcca tttactgctg aaaaacgtgc
      601 actaacttat tctctcgaga ttaatcataa acagatcgaa atcagcgctg tttccatggg
      661 aaacccgcat gctgtgcaaa ttgtaccaaa tctagagcaa gcgccagttt tgactgaagg
      721 tccgctaatt gagtcacatg cgcgtttccc aaaacgagtc aatgtcggtt atatgcaaat
      781 tattgatcgc caatcgatca aattacgtgt cttcgagcga ggagcaggtg aaacacttgc
      841 atgtgggacg ggcgcttgtg ctgcggttgt agcaggaata cagaggggtt tattgaattc
      901 taatgtagaa gttaataccc aggggggtaa attaaagata agctgggttg gtgaaaatca
      961 gcctgtatgg atgacggggc cagcaacgac cgttttcgag ggtataatag tccttccggt
     1021 caaattctaa gaaagttaaa aaaattctca catatgaagg aaagcataat gaatatagct
     1081 cataaatggc gctttttccg atccggtggc tttgatcaag ttcgtttaga aacaggggcg
     1141 gatattaaat tccttagaac acttgatccc aagttatggg ctgccttgag ttgtccaacg
     1201 aacaatctag aatttgacag taaaacgctt gaattcattg acactgatgg tgatggacat
     1261 atccgtgttc cagaaattat tgctgcagtc gaatgggcag cttctgttct taaaaatcct
     1321 gaagatctca cacaaggagc ggatgcccta caacttaacg cgattgacga tagcactcct
     1381 gaaggcgctg cactactggc atcggctaaa caaattttac tcaatattgg taaaaaagac
     1441 gaagcaacaa ttactgtcga agatactgca gatctcaata aaatttttgc cagcactaaa
     1501 ttcaatggcg acggtattat tcctgccacg gctgcaggtg acactgaaac tcaaattgtc
     1561 attgaagata tcatgaagtg tgtcggatct gtgcaagatc gctctggact cccaggtatt
     1621 tcagcagaac acattgagca attctttaca gaagccaagg catactcaga atggtggcaa
     1681 gatgcggaac gagatgctgc caacgtatta cctttagggg aaaataccga agctgcaaaa
     1741 atggcttttg atgcggtcaa aacaaaaata gatgactatt ttacgcgttg taagcttgct
     1801 gaatttgatc aaaaagcaag cgatccattg aatccttctt tattaaatta tgaagcgctt
     1861 acaaatacca atctctccag cacaacagag cagctctctg cattaccgat tgccagaatc
     1921 gaagcaaaaa aaccgcttcc acttaacgaa ggtatcaatc cggcttgggc tgatgccatt
     1981 agctcactaa aaaaacaagt tattcagccc ttattagcag ataaggagca actgagcata
     2041 gatgaatggc aagcgctttg tgacaaattt gctgcgcacc aagcctggtt aaatatcaag
     2101 cagggcgctg ccgtagaagt tcttgggatt aaacgaatcc gttcaattct ggctggaagc
     2161 taccaggata aagtcagtgc attgctgcaa aaagaccaat cccttgcgag cgcatctgat
     2221 gcaattaatt cagttgaaaa actgattcgc tatcatcgca atctttttca acttttaaat
     2281 aattttgttt cgtttcgtga tttttacaca gcgcaaaata aggcaatttt tcaggtaggc
     2341 tcgctttatt tagacggtcg tagctgtgat ttttgtctcc gtgttagcga catcaacaag
     2401 catagcagta tggctaattt aagcggtact tatctagcct actgtgaatg tcaacgtaga
     2461 ggtggtagcg agaagatgta tattgcagca gccttcacta atggtgatgc cgataattta
     2521 atggttggcc gtaatggtat tttttacgat cgaaaaggac aggactggga tgcaaccatc
     2581 gtcaagatca ttgaacatcc cattagtgtt cgtcaagcct tctggtaccc ttacaaacgc
     2641 atcggaaaaa tgattggtga gcaaatcgag aaaatggcta gcgcacggga gaaatcggtt
     2701 caagaccagg cggcttccgg catagcagat gcttcacaaa aggcagaagc tggcaaacct
     2761 ccaacacccc cctttgacgt tggaaaattt gcagggatct ttgctgcaat tggtcttgct
     2821 atcggtgcga ttgggaccgc aattgcatcg gttctgactg gcttcctcag tctcgtatgg
     2881 tggcaaatgc cgctcgcgat tcttggtttg atcctcttaa tatctggtcc ttccgtatta
     2941 atcgcttttc tgaagcttag aaaacgcaac ctagcaccat tactcgatgg aaatggttgg
     3001 gctgttaata cacgagcaat tattaatatt ccgtttggca cttcattgac tcacatggca
     3061 acattaccag ttggggcgca acgttccttg gtcgatccct atgcagagaa aaaaagcccg
     3121 tggaagactt atctattcat tctggtctta ttaggaagta ttgcttatct tttacatagt
     3181 ggacacctta atcaaggtac gattgatgag ctgagaaaac gtttctcatc caataaagca
     3241 gaagtaaccc aggaagcatc aggtactcag gaaatttcac caacaccacc ccaaacagaa
     3301 acattacctg ataattcaag aattgagact gacaataccc cccagtctgc tccaacagaa
     3361 tcaaaaagtg aatcagcagc gccttctgga ccagcctcaa ttgcgccagc accgaagcct
     3421 agtgtcgccc cctgagtgct gccgccatca ttttatgtat aagcgggcat aatcattact
     3481 atattctgac cgactgtgcc cataactctt agactctttt tacgatttta aaaccaataa
     3541 ccctacagcg agctagcgtc taagaaataa tttctaatcg aatatgcgca cactcatcac
     3601 ttatatcatg gttttcccgc ggcggagtac attcgtgctc gtcgcattgt tgtttgctgg
     3661 cgttgcggaa gcgttgagtt taacagcgct actgccattg atatcgattg ccgttggcga
     3721 aacggatgat tcaaaaattg gacgcttcat ggttcaagcg ctagagcgga ttggtattgc
     3781 tccaacaatc gatgcgatac tattaatcat cgtaggcggc atgcttatca aaggcgttat
     3841 tctattgctc accaatcgcc aagtcggtta taccgttgcc cacgtcgcaa ctgcccttcg
     3901 gttagatttg atcgaggcct tattagcgag ccgctggcaa tattacctcc gtcaacccgt
     3961 cgggtcactt gcaaattcag tggctacaga agcctatcgt gcagcaaatg gtttcgaaca
     4021 tagcgtcaat gtgctcgcac tggctatcca ggtaatcgtt cacgcgattg tggcgctctt
     4081 catttcatgg caagcaacat ttggatcatt attaattggg atcgtactat tcatcgcact
     4141 ccatcggttg gttcgcgcaa caaggcgagc aggaaccaag caaacacatc tgctacgcca
     4201 tttgttaagc tacctttccg atgtacttag ttctgtaaaa tcactaaaag ccatggcacg
     4261 cgataatgta gcggatgcca ttttacatga gcagtcaata caactcgaaa aagcaacccg
     4321 caaagaggta atcagtcgag cagccttact agcattacaa gaacccattt tagcggcatt
     4381 aactgcgagc ggactctaca tcgcactggt tgtttggaac ctttcattgc ccgaagtcat
     4441 ggtcatggta tttttgttaa cacgcatact gggcttactt aacaaaacac aacgacgtta
     4501 ccagcagctc acagttcaag aaagcgcata ctgggcactt aggactgcgg cagaagatgc
     4561 gcgtgcagct gcagaaagag ctaccggcac gatcacaccg accttacaac aaggtatcag
     4621 cctacgacat gctgcatttg attatgatcg taagccaatt tttcaagatc ttaatatcga
     4681 aattccgatt aagtcattta ctgcggtcat tggttcttct ggggtcggta aaagtacgct
     4741 actcgatttg ctctgtgggc ttgcagaacc caaatcagga gaaatactca ttgatggcgt
     4801 gcctttacat aatatcgatt tacgccagtg gcgccattta atcggctatg tatcgcagga
     4861 tacggttctg ctccatgaca caatattaaa caacacgctc gttggagcac ctgcacttac
     4921 aatcgaggac gcagaacggg cattaaagca agcaggagct tgggatttta tcagtagttt
     4981 ccccgagggc attcaaaccg ttgtcggtga gcgcggtggt atgctctcag gggggcaacg
     5041 ccaacgaatc gcgattgcac gcgccctggc tcatcaacca ttgctattgc tactagacga
     5101 acctactagc gcacttgatc ctcaaagtga gcaactcatc tgtgaaacac tcaaaaaatt
     5161 agcggaagat tatacgattg ttgcggtatc acatcagcct gcagtcatca atgctgcaga
     5221 ccgcgtgttt attctctcca atggccaagc tgatctactc accaacacgc ccaaatgatc
     5281 tactggctat tcacgattgc agcaatccgt gcttctcgaa tcgagcgatt aatcattgac
     5341 ggcaaatcaa gcaaatcgat atctttttgc ttcagattcg ctgcaataac acccgcatcc
     5401 acgcttcttg ccgcatcaaa agctttattc agtcgaactg attgcgcata aggtttttca
     5461 gcataacccg gtctcccatg aaaatcacaa gcacacgctt gcaggaatgc tgcaaagcga
     5521 tctggctttc gataggcatc ggtcgcttgt aaaatatcag caatcgttgc aggtcttaat
     5581 tcttccgctc gatgcacatc tccatgatag cgtgcaacga taagcgctaa atcacgttct
     5641 tctttcggga tacgtatgcg ctcacataaa tcttttacaa gtgtcacact tcgtgtttca
     5701 tgtcctacat gccttggcca ttcgctgggt ggtgtagtac ccttaccaag atcatgcgtt
     5761 aaggtagcaa atcgaaccgc caatgaataa ttattttgtg ctgcataatc aatggcgatc
     5821 atgatatgca cgcccgtatc gatttcagga tgatgctgag cgggttgtgg tacaccaaat
     5881 aacacatcga cttcaggcag aacccgcgat aaggcattgc aggctctcaa tgcataaaac
     5941 atctgcgaag gtcgtttttc catcagcccc cgcgcaattt cctgccatag acgctcagga
     6001 acaagtgcat cgacctcacc cgtgtgtacc atttccttca tcagctccat cgtttcaggt
     6061 gcgatatgaa aaccgaagcg tgctgcaaag cgagccacgc gcaaaattct taccggatct
     6121 tcaacgaaag caggactaat atgtctcaag attcctgctt caagatcagc aatcccattg
     6181 aatggatcga ttattttccc agtctgatct ttggctatcg cattaatggt taaatctcgt
     6241 cgcgccaagt cttcctgtaa agtaacatca ggtgaagcat aaacttcgaa tcctttgtac
     6301 cctcgggata ctttacgttc agttcttgct aaagcatatt gctcatgcgt cacgggatgg
     6361 agaaaaactg ggaaatcttt tccaacgggc cggaagccgt tttgtaccat tttctctgga
     6421 tttgaaccca cgactacata gtcgtgatct ttcaccggta acccaagcaa ttcgtctcta
     6481 actgagccac caacgagaaa aacttccata gattctactg aagtttattg aggcttcggt
     6541 gcgaccacac ctaatcgttg tctgagtggg ggaatatttt ggtgcccaaa attatttgcg
     6601 tagtagacgg aattatttaa aaccaccttc acatagtcgc gtgtttcatt aaatggaatg
     6661 gtttctgcat agatggcccc ttctaagggt ttttcaggat cgcgccactg ttttgcacgg
     6721 ccagggccag cattataagc ggcagccgct agcaaaggtt ggttatcaaa tagggacaat
     6781 acgtgtttca aataatacgt tcctaagcgc agattggtat taatatccgt cacgagatgg
     6841 ttctgaaact gttgcattcc caattttcca gcaacccact ttgcagtagc cggcattagt
     6901 tgcatcagcc ccattgctcc cgctgtcgat ttcacatcag cgagaaagcg actctcttgg
     6961 cgaattaacc cgtacacaaa cgcctcatct aattcttggt gttttaatac ctctctcatc
     7021 tgatcacgat aaggtgcaag atagcgcaag ttaaaattgt gctgtgagac ggtttgtatg
     7081 gctgtattaa ttgcacggtc ataaatccca tggcgaaatg ccacttcagc tgctgcaatc
     7141 aactgctcat ctgtaaaatt gcgaatcgtc caaacccatt cacgggctgc ctctatccgc
     7201 tggttaaatt gatataaagc cagtgcacgc tgtattcccg ggttttgttg catggcatgt
     7261 atttcttgcg agctaggatg ataattagca gcgggggaac tcatcatgac ccctaactcc
     7321 tcttttgcca tttgaccata gtagctatgt tcattgctta gcggtgccaa aatcgcattt
     7381 gcttcaccta attccccgtt tttaagtaat gctcttgctt tccagtatcg ccacacatcc
     7441 attttttgca tgctatcggg catattatcg ataatttgca aaacgtgatt ccagttgccc
     7501 ttacgaagcg caacacgccc catccacgca tgtttggctt ccgacagtgg atagggatgc
     7561 ttgtctacag cttgaataaa ccaatctaat gcacgatcat cttgtttaag cgccgcacga
     7621 taggccaggc tactcataaa aaaagattga tccgttggtg gaaaatggtt acggatttta
     7681 agcaaatgta cataagcttg atttatctca ctacgcagca atcgacctaa cgcaaacaaa
     7741 acaatttccc gatctgcacg attagaatag cttccttgaa atttctccaa atagcggagc
     7801 ggattctttg aagcttcatt caatttttgt gcattcaatt catgattacc aggtaagtag
     7861 cgactcactt gtcttgcaac gcctgtattg ccagcttcta gcgccaagcg aattcgaaac
     7921 caaatatctt catgcgataa cttctgattg gcaatgagtt gactaaaaac aggcgtacag
     7981 ctatccggca tatccttttc tgtaaaccaa agtgagcgag cctcctcaat cacctctttg
     8041 tctttactag ctaagcggag ctgaagtgcg taacaaatca atccctcatc tttattgatg
     8101 agttttggat attcttcagc aaacaaagtc cattgctggc gcttacctaa aacccttaac
     8161 caatcacctc ttagtttatc gccaatcaag ctaccttgat gacgctgaag gaatgcctga
     8221 attgaatcaa cgctcgttgt ttcaagatac aaacggattt gatagtactc ggcatagggt
     8281 gccagtgcat ggcgctgaaa gcgtggggcg tataatgcaa gcttggctgc atcacccact
     8341 cgaaaagctt cacgtagctt cagaaaatca tcttctaagc tggcagccgt tacaatcgta
     8401 ctgaccacca gcattaaaca aaatattatt tttctcatga ctcagcaata gtcagtaaat
     8461 cccgaataca aagctcagac ttactgtcag aggctcaaat cccaaaatac ctgaaaaatt
     8521 aaatctctat ttcaataaaa ttatattacc ccttgcgcaa gcattgcatc cgctactttt
     8581 acaaaacctg ctatgtttgc accatcgaca tagttaattc ttccatcaga ttgctcacca
     8641 tagcgcaagc aggaattatg aatcgctctc atgatttctt gtaagcgtgc atcaacttct
     8701 tcatgtgacc agaaaagtct catcgcttgc tgactcattt ctaacccaga tgtcgcaacc
     8761 cctcctgcat tacttgcttt accgggtgca aacaatactt ttgcatgttc aaaacgtttt
     8821 acagcacctg ccgtacaagg catattcgca ccttcagcta cacaaatcac gccattcttg
     8881 atcagcgtcg ccgcatcatc ttcactgact tcattctgag ttgcacaagg caaagcaaca
     8941 tcaacgggaa cccgccaagg tcgttcgccc ggaataaatt ttgcgccgac acgctcagca
     9001 tagtcgctta cacggccata aagatgattt tttacttcgg ctaaaatcgc caatttctcg
     9061 ggcgtgaagc cagcttcatc gacgatggtg ccactcgaat ccgaaacggt aatcactttc
     9121 gcgccaaatg ccatcgcttt ctcaactgca aattgcgcga cattaccaga tcctgaaaca
     9181 gcaacacgca gcccattcaa cgattgaccg acatgtttca gcatttcttc aacaaaataa
     9241 actgtaccat aacccgttgc ttcgggtcgt accagggaac caccgaagct aattcccttc
     9301 cctgtaaata cacaatctgc acgattcgtt aatttcttaa ccatgcctgc cataaagcct
     9361 acctcacgac ccccaacccc gatatcacct gctggcacat cggtatcaga acctacatga
     9421 cgaaatagct cgctgacgaa tgcttggcaa aaacgcatca cctcacccgg gcttctccct
     9481 ttaggatcaa aatcagaccc ccctttcccg ccacccatag gtaatgtcgt taaagcattc
     9541 ttaaaagttt gctcaaaagc caaaaattta aggattgaaa gattaacaga aggatggaaa
     9601 cgaatccctc ctttataagg gccaattgct gaactatgct gaatacgata accccgattg
     9661 acctttactt caccatgatc atccacccag gcaactcgaa agataatgat tcgttcaggt
     9721 tcgacaattc tgtctaataa gccatgctca gcataccgag ggttattggt aataaaaggc
     9781 cacaaactct ctattacttc tgtaacagcc tgcatatatt caggttggcc tggattacgt
     9841 tcagaaacat aatttctgaa ctcttctatt cctttatatt tcattctatt ttctcctctc
     9901 tgcttgatca gcgttcgtta ttccccaaaa ctttctgcca ttaatccaat ttttttctct
     9961 gattttaatg aattcttttt agtcacaaat acaaagtgta actggattac ttgcaatatt
    10021 acagccatct tttatttgcg tgatgaaatc ttgattgatt ctatttatat ctagattttg
    10081 gtcggttagg cttattcaac aattctttgt agcaatgcct taaattacaa tctcaaaatt
    10141 aagaaacctg aattttacct ttccatagca ttgcaaagct ggtcagcatt cattgaaata
    10201 acctaatcaa cgggtctgct aatcaaatat ttccaaagga acaaaaagta attttacccc
    10261 ggaaggaggc tcagtttgag tccatgcaat gataactgag gatcgattct taactatttg
    10321 aggcactccg ctatcacgac ctggattgat ttcacttaag cgtttgatct gtcccatatt
    10381 tccattcata ccaatgcttc gagctgccac gctcgccttc cgagttacct tatccattgc
    10441 cgttatccaa atcacaatgg ctgtctcatc agtaagcgct ataacgcgtg ttctgccgat
    10501 cggtttttca tcatcaatat ccaacgcttg attgaattgc aagctggaat cattggcaaa
    10561 agcactgcgt acacgcggac gatttccttc agcggtaaac caacttgcag ccacgtattt
    10621 tccgtttgta gcgatagagg ggccattaac tgggcatcct gcaatagacc acccctcctg
    10681 gctgacgggt tgcggatctg accatatgcc atcgatcaac tgcgcaaaat gatgatctcg
    10741 aatttcatcg ccacgccgac ttcgccaaac tacaacgggc cctttcgaag tcactgccga
    10801 agcagtccag cagcatgtgc acgtattgtc atcaatcact cgatctgagt caatttgccc
    10861 attccgatga attaaggcat agcgcaacga aaagcgatca gagatttttt ttgcaacgtc
    10921 tctcccatcc aaccagacta taccagcctg acctagcgca ggaaaaacag aaacgaatcc
    10981 atgggctgca gccgtatcat cttgatatgg cttctgcggc gcactccatg ttacaccggc
    11041 atcgacagaa atcgagaaaa acacatcgta gtcataaatc tgctcagttt tagatttaac
    11101 gcgccagtga gctacccaga atgaatcatc gattgcaaca accgcgggat aatcagccca
    11161 attcacatac caccccgagc cttgtacagc ttgaccgata cgcacccaac gatcctggcg
    11221 atagatagca aattttaaaa cctggtgatt tccttttcct tcgatccaac tcatgagcat
    11281 tgaaccatcc ggcaaggaag aaagatacgg gaattgagat cctattgctg caggattctc
    11341 aatgaatcga gactgttgag atgattgatc aaaagtttgg cacgcgctaa tgatccatga
    11401 caaattgaaa acaatgatga ttgtcgagca atagcgaaaa aagtctttaa agacaagtcg
    11461 catagcataa ctctttgctg tattaatgaa gaagaattac caacctggtt caaccaattt
    11521 tagtaaagat gatgaatccg gaaaatgtgg caaattgtcg cacgattcat atgaccttcc
    11581 aacaaaaaca ggttctcact accgcattac gttagtacta gctagattcc ccctcgagca
    11641 ataaaccagg atcaatcgta gcttgttcga tttcgacaag tttatagcga gaatgcgctc
    11701 ctcgttttat gcttacttga ttggcagaaa cttgaaaaca atccgctaaa aacttaatga
    11761 gcgctttatt cgccttacct tctataggcg gtgctgcaag actgacctta agggcatcgc
    11821 catgcaagcc tgcaaattgc gtattttttg tgcctggctg gatgtaaaga gataaaatta
    11881 gtttgccatt attttctcgg cggtaccaat tcattaaaac aagaccgcta tttcctgctg
    11941 caatccagtc aaaaccatta gcaataattg cactgccacc agtacaaata gtggggataa
    12001 gtcgacattg gcaatcggcg gaataaatcg ttggaaaata cctaaaattg gacgtgtaaa
    12061 gctcgtaagc aaaggtgcaa ttgggctata agggttaatc caagaaagaa ttgcttgcac
    12121 gataaccatg gccatcacga tgtaaagtgt caggcgaaca agctctaaca gcgataacaa
    12181 gccaaaccct atcatcgtat tgcctgatac cgtctctaat tcatagcctt gaataaaact
    12241 agtactgacc atgataagaa actgtcctat ccacgctaaa aaaaatgtgg atagatcaaa
    12301 tcccttccaa cttggaatga ctcgacgggt aggtagaacg ataaaattcg tcacagcaat
    12361 caaaaatttt gatagcgggt tatcgtagtg aactcgaaat aattgcaaat aaaatcttag
    12421 taagcacgcc agagaaaaaa gaccaagtat cgtctccaac aggaatatca atatttgact
    12481 agacattgtt caatctccaa agaggttcat actcaaaatt tcaaccatct acctgaccta
    12541 gttcatcacc gagctgctga gcgcgtttat aggcagcaaa cgctgcccga ataatttcat
    12601 tcttaacatc atcttggctc atactg
//
LOCUS       DAOQLJ010000060        12576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1910_length_12576_cov_5.147193, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000060 DAOQLJ010000000
VERSION     DAOQLJ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12576
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1910_length_12576_cov_5.147193"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PK522_05770"
     CDS             <1..766
                     /locus_tag="PK522_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNC41274.1"
                     /translation="AIPYIGDTLSQMILGDFTLSDAALNRFFAYHVVTLPLLLLVLVF
                     VHIVALHEVGSNNPDGIEIKSNLDSKTGTPVDGIPFHPYYSVKDIFGVVAFLIVFCGI
                     IFFAPEFGGYFLEYNNFIPANTLQTPDHIAPVWYFTPYYSMLRAVTVNFLGIDAKLWG
                     VILMAMAVVIFCFLPWLDRSPVKSIRYKGPYFKFALTLFVISFFVLGWLGTKAPTPLY
                     TLLAQIFTVIYFAFFLLMPWYSKIDKTKPEPKRVTK"
     gene            763..1488
                     /locus_tag="PK522_05775"
     CDS             763..1488
                     /locus_tag="PK522_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HNC41275.1"
                     /translation="MTLIRSFLIIFLFFPIGINAAGVEVQLDKAPIDTRDNDSIKRGA
                     QSFVHYCLTCHGASFMRYNRHQDVGFTNQELLDNIIQTGQKVGDLMVSAMKKNEAEGW
                     FGVAPPDLSVIARSRGADWLYTYLRSFYRDETTATGWNNLIFDKVAMPHVFYELQGQQ
                     ILKVKSLGDGKESKTLELAVPGSIPKAEYDKFVADLVNYMVYLGEPHAAHRVDLGIKV
                     MLFLFAMLGLTYLLYKEYWRDIH"
     gene            1561..2160
                     /locus_tag="PK522_05780"
     CDS             1561..2160
                     /locus_tag="PK522_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNC41276.1"
                     /translation="MMTLYSTATCPFSHRCRIVLHEKDMDFQIHDVDPNNKPEDLAVI
                     SPYGQVPVLVERDLVLYESNIINEYIDDRFPHPQLMPAEPVMRARARLLLHRFEQELF
                     CHIEVAEQNDHKLTDSARKIIAENLTMIASLFDKQKYMLGDEYSMLDVAIAPLLWRLD
                     YYGIRLPKQAAPLLKYSERLFSRPLFIDALTPSEKMMRK"
     gene            complement(2183..3133)
                     /locus_tag="PK522_05785"
     CDS             complement(2183..3133)
                     /locus_tag="PK522_05785"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HNC41277.1"
                     /translation="MSISMLNKQEALQAIIDIVRNHQLSNKEVIAALTDTKNITKQRT
                     TGVLSRIFGYIGSILVFSGICIFIGMRWEQLGAVERITATLGIGFMLFLIAVAVMDNP
                     KYDRTATPLLLIAALLQPTGIFVLLDEFSRGDDPLYGVLFMSVIMFIQQGFIFLQKGR
                     TTLAFTTILFGSMFIITTLELLEVDGNVISFMIGLSLMFISWALGHSPHRAISSFWYF
                     FGSISLLISAFDFLKNQTYEVLFLGLAAIIIYVSIAAQNRTLLLVGTLAMLSYISHYT
                     YIHFNDVLGWPIVLIICGVAFIAISAVAMKINAKFMSTPS"
     gene            complement(3130..3720)
                     /locus_tag="PK522_05790"
     CDS             complement(3130..3720)
                     /locus_tag="PK522_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41278.1"
                     /translation="MKQNFLKENFALIIGLGLPVVLVIGFFFARYLPQQYSDEPPSYE
                     LVFSVMRYDNYSPFHVDFIVKDQKLYMKLINKKEHEGANVRELFIYNGQKGSTRKIHY
                     SLPDHLVNLQNEILVKEFSAHVIDTNSKSPDGYELEPASYRSRGLLGELFGSNTGKYF
                     HRVKKAGGKSFPISDYGSEFSYGNLSFIGWIIQSNQ"
     gene            4044..6437
                     /locus_tag="PK522_05795"
     CDS             4044..6437
                     /locus_tag="PK522_05795"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNC41279.1"
                     /translation="MLHHIQIPIDGMTCAACAARLEKNLNKLQGVNASVNFANEKANI
                     DYEESLTQPQAIIQSIKDSGFNVLPQTLQLKIDGMTCAACSGRVESALNKLPGVTANV
                     NQATEVAYITYTPGIVSLENLIEAVTDSGYHADKLNQANRDKENSRKQAIYQDDLRLF
                     WFSAILTIPLMLQMLTMFSDDHSEWLPRWLQWLLATPVQFWIGFRFYKGAWYALKGGA
                     ANMDVLIVLGTSMAYLFSTVVTLYGLEQHVYFEASAAIITLVLLGKLMESRAKNKTST
                     AIEELIRLQPTIAHVQKENGEILDLDINNLSVNDIFIVRPGESLPVDGIVIEGDSAIN
                     EAMLTGESLPVTKRVGDNVYAATINQQGLLKCRATGVGSETQLAEIIRLIERAQGSKA
                     PIQRIADKIAGIFVPIVMGISLLTLSLTWWLEGEFVLALINAVSVLVIACPCALGLAT
                     PTAIMVGTGRSAQMGVLVKNAAALEHAEKIQVLVVDKTGTLTMGKPVVTNIIPIGKTD
                     QTTLLQIAALIEQNSEHPLAKAILDQAIGINKLEQKITNFQAITGRGVKAEIDGIAYI
                     LGSPKFLGEYGVENDHIEIKKLQLEGKTLVGIAMQLDHRYQSLGYFAIADQLRETSKQ
                     AIQRIQAMGIEVIMLTGDNAATATTIAKQAGIMQYRAEVLPQDKAAVVSEIKSQGKYT
                     GMIGDGINDAPALAAADVSFAIAAGSDVAIEAADITLMRNDLMSVADAIALSRATLRK
                     IRQNLFFAFIYNVLGIPLAAVGMLNPVIAGAAMAMSSVSVVSNSLLLKRWNMSRYNK"
     gene            6485..6694
                     /locus_tag="PK522_05800"
     CDS             6485..6694
                     /locus_tag="PK522_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HNC41280.1"
                     /translation="MESKTVYIKGMNCMGCVSSIKNVLEKLPGVESAEVSLEDACVTI
                     HYDAASIGIDQLKKAIDNAGFEAIT"
     gene            complement(6703..7434)
                     /locus_tag="PK522_05805"
     CDS             complement(6703..7434)
                     /locus_tag="PK522_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNC41281.1"
                     /translation="MKIFWIVSALLILNACASTSRSIQDNPTLPIESPNNEIALDNIN
                     RSVPTSTATEVEQNNPTVALTKGEDRVTSSHKATSKPEWPIETGVATYYANQMEGQLT
                     ASGELYDSAQLTAAHRTLPIGSQVRVTNAKTKQMVIVRINDRWGGGGDRIINLSKAAA
                     TELGFGSAGMLTVTLSVESIPSGSNQQFSEEKPLQAKQLPSRIADDSSANHPKLRICQ
                     NEANILGLTGEFYRYHVSACLGRNK"
     gene            complement(7548..9620)
                     /locus_tag="PK522_05810"
     CDS             complement(7548..9620)
                     /locus_tag="PK522_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNC41282.1"
                     /translation="MPILRTPLLFKILSAIVLTHLATIVLAAHELNNVPQQKTNNLMD
                     MDIEKLMSLEVTTVSRRSQKLAEVASAIFVITQDDIRRSGVTSIPEALRMAPGVEVAR
                     IGTDKWAIGIRGFNGRFADKLQVLMDGRSVYNPLFAGVQWEQQDTLLEDIERIEVIRG
                     PNASVWGANAVNGIINIITKKAAQTQGLLLTAGGGSFERGFFGARYGGTINDNTHYRF
                     YTKGFTRDPMNSISGANANDAWHSARGGFRLDHSRGIDQFTAQGDIFYNAIGDTFDKT
                     LLGPPIIEPTAARGHNEGGNIRLRWDRTLSEKSSIMFQTYYDRVDYKLLTSTQFRAES
                     FDIDFQHRFPLWNRHDLTWGANYRLYHNKVFDTEVIALSPRQQTNHLASVFVRDEMTL
                     IPEYLRFTLGTRIDHNDFTGFEIQPNARLMWTPNPENSLWASVSRAVRTPSRAENDIT
                     LNTRILNSIPGTNTPIPFPVLAQIAGNSNYNSEKMIAYELGYRHQISQTASIDIAGFF
                     NDYSQLRDLASPAFKDISVHSSFPSHLIYPVGLNNNASGHTYGVELSADWKPTQRWRL
                     QGNYSYLHMHINSNSTFKSIDPTTGGADKVSPNHQISFRSHYDLTERMQFNLWLRYIS
                     GIDFYQIPGYVTMDTKIAYKPIKNLEVFLVGQNLFSENHREFVSDFIPSLPLYIPRGI
                     YVGGEWRF"
     gene            complement(9622..10188)
                     /locus_tag="PK522_05815"
     CDS             complement(9622..10188)
                     /locus_tag="PK522_05815"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNC41283.1"
                     /translation="MLVQLIGNSAKNNSITMQQLLLKAWLLLLLTFPLPSIADTLDEY
                     QVKAAFIYNFIAFTQWPYEINQPLNFCIYGEDYFGQEIDHLQVKQINNQRIHVLRFSQ
                     LEEIKVCQVLFISKSNISSLPSILAFIQGQSILTIADTPDAAIKGIMINMIVAQNRIL
                     FEINLQSARNAKLDISSKLLQLAMKVYQ"
     gene            complement(10250..12169)
                     /locus_tag="PK522_05820"
     CDS             complement(10250..12169)
                     /locus_tag="PK522_05820"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC41284.1"
                     /translation="MKVADIKQLNISALLPSPKGVALALLEVCRKEDATLGEITKLIQ
                     TDPALSGRLIHKANLASQSARPITSVTEAVSRVGLTVVKQLALGFSLIDQYQGGPCKG
                     FNYQQFWSHSLLMAIAMQELGKSVRVCPPDELFACGLMTRIGCLALATIYPGKYTEIL
                     EKQDTNISLPLLEQQFLETDHNQLTAAMLINFGVPNIMVEPIYYHESPHESGFSEGSR
                     PYQIVHLLYLAKQIADLGISPESEHSKRTADLMLLGSKIGLDTESFGQFIDQIMHNWQ
                     QWGEMLKLPAVAPPSFQKMASTPAPRPEESIDAATLRVLLVEDELSSRLLTEGILNNI
                     LGHTVHTAVNGKEALSLAIEVQPHIIITDWIMPAMDGLELTRAIRSTDWGQNIYIIML
                     TSLENEEDIVEAFDAGVDDYVSKPINLRAFRARLRAAWHYRKLQESWERDREQLKRFA
                     AELAVTNRKLEHIALTDMLTELPNRRAGMNVLSETWSNSERANQSMAVMLIDIDYFKK
                     INDTYGHATGDRVLIEIATLIRSNARKGDSFCRMGGEEFLVICHSGCIEMKSTVQFAE
                     RLRQYTKSHPIKIDENNIEVTISIGIAQKETSVENPDQLINLADKALYAAKNAGRNKV
                     FLAAENKLINCSNQS"
     gene            complement(12150..12515)
                     /locus_tag="PK522_05825"
     CDS             complement(12150..12515)
                     /locus_tag="PK522_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC41285.1"
                     /translation="MHIALDDFGTGYSSMNYLKRLPIDSIKVDQSFVSGLLDDKDSLA
                     IVRSIVTLSKNLGFHITAEGIETLNQAEILKFLGCETLQGFYFSKPLHKNDMLQLSNK
                     SWDIDAVNPLGYHYESRRY"
BASE COUNT         3701 a         2436 c         2640 g         3799 t
ORIGIN      
        1 cgctattccc tacatcggtg atacgctctc acaaatgatc ctgggtgact ttactctctc
       61 agatgctgcg ctaaatcgtt tctttgctta tcatgtagtg acgttgccat tgttattgct
      121 tgtattggtt tttgtgcata ttgttgcttt acatgaagta ggatcgaata atcctgatgg
      181 aattgaaatt aaatcaaatc ttgattctaa aacaggtacc cctgtcgatg gaattccatt
      241 tcatccttac tactcagtta aagatatttt tggagtcgta gcatttctaa tcgttttctg
      301 tggcattatc ttttttgccc ctgaatttgg tgggtatttc ctggaataca ataattttat
      361 cccagccaat acactacaaa cacccgatca tatcgcacct gtttggtatt tcacgcctta
      421 ctattcgatg ttgagggcag taacggttaa tttcttagga attgatgcta aattatgggg
      481 tgttatctta atggcgatgg ctgtggttat tttttgcttc ttgccttggc ttgatcgtag
      541 tcctgttaaa tcgattcgtt acaaaggtcc ttatttcaaa tttgccttaa cgctttttgt
      601 aattagcttc tttgtattag gttggttggg taccaaagca ccaaccccat tgtataccct
      661 attagcgcaa atttttacgg ttatttattt tgctttcttc cttctaatgc cgtggtatag
      721 caaaatagat aaaactaaac cagaacctaa aagagtgaca aaatgacatt aatacgatcg
      781 tttcttatta tctttttatt ttttccaatc ggaattaatg ctgctggtgt agaagtacag
      841 cttgataaag cgcctattga tacacgtgat aatgattcaa taaaacgagg tgcacaatcg
      901 tttgttcatt attgcctaac ttgtcatggt gcaagtttta tgcggtataa ccgccatcaa
      961 gacgttgggt ttaccaatca ggagctgctt gacaatatca ttcaaacggg tcagaaagtg
     1021 ggtgatctaa tggtcagcgc gatgaaaaag aatgaagctg aaggttggtt tggagtagca
     1081 ccacctgatt tatcagtaat tgcgcggtca agaggtgcag actggctata tacttattta
     1141 agatctttct atcgcgatga aacaacggcg acagggtgga ataatttgat atttgataaa
     1201 gttgcgatgc cccatgtttt ctatgaatta cagggacaac aaatactcaa agtcaaatca
     1261 ctcggtgatg gtaaagaatc gaagactctt gaactggctg tacctggttc tattccaaaa
     1321 gctgagtacg ataaatttgt agctgacttg gtcaactata tggtttatct tggagaacca
     1381 catgctgcac atcgtgtcga tttaggaatc aaggtgatgt tattcttatt tgcgatgctt
     1441 ggactaacct atttactgta taaagagtat tggagagata tacattaaga atcaaatagc
     1501 gttaacagtt tgtactcgta tctcttttaa tagcctatag tataagattg ctgaaaagtt
     1561 atgatgacac tctactcaac ggcaacgtgt ccttttagtc atcgttgtag aatagttttg
     1621 catgagaaag atatggattt tcagatccat gatgttgatc ccaacaataa gcctgaagat
     1681 cttgcagtaa taagccctta tggacaagtt cccgtgttag ttgaacggga tcttgttctt
     1741 tatgaatcga atattatcaa cgagtatatt gatgatcgct tccctcatcc gcagttaatg
     1801 cctgctgaac ctgttatgag agcaagagcg agattactgt tacatcgttt cgagcaagag
     1861 ttattttgtc atatcgaagt tgctgaacag aatgatcata aattaactga ttcggctcga
     1921 aagatcattg cagaaaactt gacgatgata gcttctcttt tcgataagca aaagtatatg
     1981 ctgggcgacg aatattcgat gctggacgtt gcaatagcac ccttgttgtg gcgactagat
     2041 tattacggta ttcgtttacc caaacaagct gcacctttgc ttaaatattc agagcgctta
     2101 tttagtagac ctctgtttat cgatgcatta acgccatcag aaaaaatgat gcgcaaatag
     2161 cgttaatatt gttgtgtttt tgctagctag gcgtgctcat gaatttggcg ttaattttca
     2221 ttgcaactgc gctaatcgcg ataaaggcga ctccacaaat aataagcacg attggccaac
     2281 ctagtacatc attaaagtgt atgtaagtat aatggctaat ataactcagc atagctaaag
     2341 tacctactag caataacgtt ctgttttgag ctgcaatact cacgtagatg attattgcag
     2401 ccaagcctaa aaatagtact tcataggttt ggttcttaag aaaatcaaaa gctgaaatta
     2461 acaaactgat cgaaccgaaa aaataccaga atgacgatat ggctcgatga ggtgaatggc
     2521 caagcgccca cgatataaac attagcgata atccaatcat aaagctaatt acatttccat
     2581 ctacttctaa taactctagt gttgtgatga taaacatgct tccaaaaaga atcgtcgtaa
     2641 acgcaagcgt tgttcttcct ttctgcaaaa atataaaacc ttgctgaata aacatgataa
     2701 cggacatgaa taaaacccca taaagaggat catctccacg tgaaaactca tcaagcaata
     2761 caaaaattcc agtgggttga agtaaagctg caattagcaa taggggtgta gcagttcgat
     2821 catattttgg attatccata actgcaacag cgattaggaa taacataaaa ccgataccca
     2881 gtgtcgctgt aattcgttcg actgctccca gttgctccca tcgcatccca ataaagatgc
     2941 aaattcctga aaatacgaga atacttccaa tataaccaaa gattctagaa agaacccctg
     3001 tcgttctctg tttcgtgatg tttttcgtat cagttaaagc agcgataact tctttatttg
     3061 ataattgatg atttcttacg atatcgataa tcgcttgtag cgcttcttgc ttgttaagca
     3121 tagatatact cattggtttg attggataat ccaaccgatg aaactaaggt tgccataaga
     3181 aaattcactt ccataatcag aaatcggaaa gctttttccc ccagcttttt ttactcgatg
     3241 aaagtatttt ccggtattac tgccaaatag ctcgcctaat agtcctcgtg aacgataact
     3301 tgccggctct aattcataac catcgggaga tttgctgtta gtatcgatca catgcgctga
     3361 aaattctttc actaaaattt cattttgtaa attgaccaaa tgatccggaa ggctatagtg
     3421 aattttgcga gtacttcctt tctgtccatt gtagatgaac aattctcgga catttgcacc
     3481 ttcgtgctct tttttattga tcaatttcat ataaagcttc tgatctttaa caataaaatc
     3541 gacatgaaag ggtgaataat tatcatagcg catgacgcta aataccaatt catagcttgg
     3601 cggctcatca ctgtattgtt gtggaaggta tcttgcaaaa aagaaaccaa tcactaaaac
     3661 aactggtaac cctaatccga tgattaaagc aaagttttcc tttagaaaat tttgtttcat
     3721 tagaatcctt taatgcaaaa atttaagctt actatgctag aaaacaattg aaagtaaaaa
     3781 aatggagctt attttgtatt aaatcaattt aaaacattac agttaggaaa ctaacctgaa
     3841 ttgataaata attttatgaa gttttattga ttttcaagaa tcatgattta gctagtcgtg
     3901 ctgatcagca ttagcctcca ctcttttagg ttttgaggaa tctttaatct ggaatgttta
     3961 gaatcagttt tgctgtattg aattaccata ttaaagagag tacggtaaca attgatagaa
     4021 aaatttattg aaataaaact acgatgcttc accacattca aataccaatc gatggaatga
     4081 cttgcgctgc ctgtgcagcg cgactcgaaa aaaatttaaa taagttacaa ggagtcaatg
     4141 cgtccgttaa ttttgctaat gaaaaagcaa acatcgatta cgaggagtcg ttgacgcaac
     4201 cccaagcgat tattcagtcg attaaagatt caggtttcaa tgttttaccg caaacgttac
     4261 agcttaagat agatggtatg acatgtgccg catgcagtgg gcgtgttgaa tccgccttaa
     4321 ataaattacc gggtgtaacg gccaatgtga atcaagcaac ggaagtggcc tatataactt
     4381 atacgccggg tattgtttca ctggaaaatc tgatcgaagc agttaccgat agtggttatc
     4441 acgcagataa attgaatcaa gccaatcgtg ataaagaaaa ttcgcgaaag caagctattt
     4501 accaggacga tcttcgctta ttttggtttt cagcaatttt aacgatacca ctgatgttgc
     4561 aaatgctgac aatgttctct gatgatcata gtgaatggtt accgcgatgg ctgcagtggc
     4621 ttcttgcaac cccagtccag ttctggattg gtttccgttt ttataaaggg gcttggtatg
     4681 ctctaaaagg gggggctgct aacatggatg tcctcatcgt actgggtacc agcatggcct
     4741 acctatttag cacggtagtt acactctacg gattggaaca acatgtttac tttgaagcca
     4801 gtgcggcaat tattaccctt gtattactcg gaaaattgat ggagtcccgt gctaaaaata
     4861 agacttctac cgcaattgaa gaattgatta gattacaacc aacaatagca catgtgcaaa
     4921 aagaaaatgg tgaaatacta gatttggata tcaacaacct aagtgtgaat gatatcttta
     4981 ttgtccgtcc tggggaaagt ttacctgtcg atggtattgt gattgaagga gattccgcaa
     5041 tcaatgaagc gatgcttacc ggtgaaagtt taccagtaac aaaacgggta ggtgataacg
     5101 tttatgctgc tacgattaat cagcaaggat tattgaagtg ccgcgccact ggtgttggat
     5161 cagagacaca gctcgccgag attattcgtt taattgaacg cgcacagggg tcaaaagccc
     5221 ccatccaaag aatcgctgac aaaattgccg gaatctttgt accgattgtg atggggatca
     5281 gtttattaac gttatcgcta acctggtggt tagaaggtga atttgtctta gcgcttatta
     5341 atgctgtttc agtattagtt attgcctgtc cctgtgcatt aggtttagcg acgccaacag
     5401 cgattatggt gggaacgggt cgcagtgcgc agatgggtgt attggtaaaa aatgcagccg
     5461 cccttgagca tgctgagaag attcaagtgc ttgtcgttga taaaaccggg accctaacga
     5521 tgggtaaacc tgtggtaacc aatattattc cgattggaaa gactgatcaa acgacgttat
     5581 tgcagattgc agcgcttatc gagcaaaatt ctgaacatcc actcgctaaa gccatccttg
     5641 atcaagccat cggtatcaat aaactggagc agaaaatcac aaattttcag gcaataaccg
     5701 gtcgcggggt aaaggctgaa attgatggta tcgcatatat tctcggctca cccaaattct
     5761 tgggggaata tggtgttgag aatgatcata tagaaataaa gaaattacaa ttggaaggta
     5821 aaacgcttgt cggaattgcc atgcaactcg atcatcgcta ccaaagctta ggttatttcg
     5881 caatcgctga tcaattgcga gaaacttcaa agcaagccat tcaacgcata caagcaatgg
     5941 gtatcgaagt gatcatgcta acgggtgata atgctgctac agcgactacc atagcgaaac
     6001 aagctgggat catgcagtat cgtgctgaag tgttgccgca agataaagct gcggtggtta
     6061 gcgagataaa atcccaagga aaatataccg ggatgattgg agatggcatt aatgatgcac
     6121 ccgctttagc ggcagcggat gtcagctttg cgattgccgc aggatcagat gttgcaattg
     6181 aagctgcaga tattacactc atgcgtaatg atttaatgag tgtcgccgat gcaattgcac
     6241 tttcgcgcgc tactttaaga aaaattcgtc aaaatctctt ttttgctttt atctataatg
     6301 tgctcggtat tccattagct gcggtaggta tgctcaatcc tgttattgca ggtgctgcca
     6361 tggcaatgag ctctgtgtcg gttgtgagta attctttact gcttaaacgt tggaatatga
     6421 gtcgctataa taaatagtta aaagatagat agttttaggt tgaatgatga tgggagtaat
     6481 gaaagtggaa agtaaaacgg tttatatcaa aggaatgaac tgtatgggtt gtgttagtag
     6541 catcaagaat gtattagaaa agcttcccgg tgttgaaagc gctgaagtat cgcttgagga
     6601 tgcttgtgtt accattcact atgatgcagc ttcaattggc attgatcaat tgaagaaagc
     6661 aattgacaat gcgggttttg aagcaattac ctaaataaat ctctatttgt tgcgacccaa
     6721 gcacgcagat acgtgatatc gatagaactc acccgtcaac ccgagaatat tcgcttcgtt
     6781 ttgacaaata cgcaatttag ggtggttcgc tgagctatca tctgctatcc gagaaggaag
     6841 ttgttttgct tgcagcggct tttcttcgga aaactgttga ttgctacctg aaggtataga
     6901 ttcaacgctt aatgtgacgg ttagcatacc cgcacttcca aaaccaagtt cggttgctgc
     6961 cgctttagat aaattgataa ttcgatcgcc acctcctccc cagcgatcat ttatgcgaac
     7021 gattaccatt tgtttggttt tagcgtttgt gacacgaact tgacttccga ttggtaacgt
     7081 gcgatgcgct gcggtgagct gcgcagaatc ataaagctca ccactggcag ttagttggcc
     7141 ttccatctga tttgcgtaat aagttgcgac tccagtctca attggccatt caggtttaga
     7201 agtagcttta tgactactcg ttacacgatc ttccccctta gttaaggcca cggttggatt
     7261 attttgttca acttcagtag cagttgaagt gggaacggaa cgattgatat tgtcgagtgc
     7321 gatctcatta ttggggcttt caatcggtaa tgtaggatta tcttgaatgc tgcgagaagt
     7381 acttgcacat gcatttagaa ttaagagcgc actaacgatc cagaagattt tcatgactgt
     7441 tattattgtt ttcactgaaa taagatatta ggctttcaat aatacctgat caatggttaa
     7501 aactcactat taagttcatt catcacactg ggtttatagt tatcttgcta gaagcgccat
     7561 tcgcctccga cataaatacc acgtggaata tacagtggta atgaagggat aaaatcggat
     7621 acaaattcac ggtgattctc gctaaataaa ttttgaccga ctaggaatac ttctagattt
     7681 ttaatgggct tataagcaat tttagtgtcc atcgtgacat aacccggtat ttggtaaaaa
     7741 tcgataccgc tgatatagcg aagccagaga ttaaactgca tacgctcagt taaatcgtag
     7801 tgagagcgaa atgagatttg gtggttaggg ctaactttat ctgcgccacc ggtcgttgga
     7861 tcaatcgatt taaaagtaga gttagaattg atatgcatat ggagatagct gtaattccct
     7921 tgtaagcgcc atctttgtgt cggtttccaa tcggctgata attcaacgcc ataggtatgc
     7981 cctgaagcat tgttatttaa tcctaccggg taaatgagat gcgaaggaaa gcttgaatga
     8041 acagaaatat ccttaaaggc tggtgaagct aaatcacgca gttggctata atcattaaag
     8101 aagcctgcga tatcgattga cgcggtttgg gaaatttgat gtcgataccc taattcatac
     8161 gcaatcattt tttctgaatt atagttagaa ttccctgcga tctgagctaa aacggggaat
     8221 ggaataggcg tgttggttcc tggaatcgaa ttaagtatgc gtgtatttaa cgtaatatca
     8281 ttctcagctc gcgaaggcgt tcgtacggcg cgagaaaccg atgcccatag cgaattttca
     8341 ggattcggcg tccacatcaa ccgagcattc ggttgaatct caaacccggt aaaatcatta
     8401 tggtcgattc gtgttcctaa tgtaaatcgc agatattcgg gtattaaagt catttcatca
     8461 cgcacaaaga cgctcgcaag atgattcgtt tgctgacgtg gggaaagtgc gatcacttcg
     8521 gtatcaaata ctttattgtg ataaagccgg taattagcgc cccaagtcaa atcatgacgg
     8581 ttccaaaggg gaaatcgatg ctgaaaatca atatcaaagc tttccgcgcg gaattgagta
     8641 gacgttagta atttataatc aacccgatcg taatacgttt gaaacataat ggatgatttt
     8701 tcagaaaggg tgcgatccca gcgtaaccgg atgttacccc cttcattatg tccccgtgca
     8761 gcagttggct cgataattgg gggacccaat agcgttttat caaacgtatc accgattgcg
     8821 ttataaaaaa tatccccttg cgcggtaaac tgatcaattc ctcgagaatg atctaaacga
     8881 aatccacctc gggcactgtg ccaagcatca ttggcattag ctccggatat ggaattcatt
     8941 ggatcgcgtg tgaatccttt cgtataaaaa cgataatggg tattatcatt gattgttccc
     9001 ccatagcgtg caccaaagaa accgcgctca aagctaccac caccggccgt caacaatagc
     9061 ccttgggttt gagcggcttt tttagtaatg atattgatga taccattgac ggcattagcc
     9121 ccccagacag aggcgttcgg tccacggatg acttcgatcc gctcaatgtc ttcgagtaaa
     9181 gtgtcttgct gctcccactg aacgcctgca aataaagggt tgtacacact tcggccatcc
     9241 atcagtactt gtagtttgtc ggcaaaacgt ccattaaatc cgcgtatacc aattgcccat
     9301 ttatcggtac caatccgtgc tacttctaca ccaggcgcca tccgtaatgc ctcaggaata
     9361 ctggttacac ctgagcggcg aatatcatct tgggtgatca caaatatagc tgatgcaacc
     9421 tctgcaagct tttgcgagcg ccttgagacg gttgtgactt ctaaactcat cagcttttca
     9481 atatccatat ccattagatt attggttttt tgctgtggaa cgttgttcaa ttcatgtgct
     9541 gctagaacta tcgttgccag atgagtcaat acaattgctg agagtatttt aaacaatagg
     9601 ggggtacgca atattggcat gttattgata taccttcata gcaagttgta ataattttga
     9661 gctaatatcc aatttcgcat ttcgcgctga ctggagatta atttcaaaca aaatccgatt
     9721 ctgtgccacg atcatattga tcatgattcc tttgatagca gcatcgggag tgtcagcgat
     9781 ggtgagaatg gattgacctt gaataaaagc tagaatagag gggaggctgc tgatatttga
     9841 ttttgatata aataaaactt ggcaaacctt aatttcttca agctgggaaa atcgtaagac
     9901 atgaatgcgt tggttattaa tctgctttac ttgtaggtga tcgatttctt gtccaaaata
     9961 atcttcacca tagatacaaa aattgagggg ttggttaatt tcataaggcc attgggtaaa
    10021 ggcaatgaaa ttgtaaataa atgcagcttt tacttgatac tcatcaagcg tatctgcgat
    10081 cgaagggaga gggaatgtaa gcagtaacaa taaccatgct ttcaatagta attgttgcat
    10141 cgtaattgag ttattcttcg cgctatttcc tattaattga acaagcatcg atgaatttga
    10201 tacgcaatga atgactatgc cttcaccaca ctatgtagca atatctgtat taactttgat
    10261 tgctacagtt aatcagctta ttctcagccg ctagaaatac tttgttccta cccgcatttt
    10321 tggcagcata gagtgcttta tccgccaaat tgattaactg gtctggattt tcaacactgg
    10381 tctctttttg agcaataccg atactaattg tgacctcaat attattttca tcgattttga
    10441 tcgggtgaga cttagtgtac tgacgtaatc gttctgcaaa ttgcacggtc gatttcattt
    10501 caatgcagcc actgtggcaa ataactaaaa attcttcccc ccccatgcgg cagaatgaat
    10561 cccctttacg ggcatttgat cggattaacg tagcaatttc gatcaacaca cgatcacctg
    10621 tcgcatggcc ataagtgtca ttaatcttct taaaataatc gatgtcaatt aacatgacgg
    10681 ccatggattg attcgcgcgt tcactattgc tccaagtttc agatagaaca ttcattcccg
    10741 cacgacgatt aggcagttct gttagcatgt ccgtcaaggc aatgtgctct aattttcgat
    10801 tggttactgc caattctgct gcaaaacgtt tgagttgttc cctgtcacgt tcccaagatt
    10861 cttgtagttt ccgataatgc caagcggctc gaagtcgggc acgaaaagct ctaagattga
    10921 tgggcttaga tacatagtca tctacaccgg catcaaatgc ttcaacaata tcttcttcat
    10981 tctcgaggct agtgagcatg ataatgtaaa tattttgccc ccaatccgtt gaacgaatag
    11041 cccgagttag ctccagcccg tccatggcag gcataatcca atcagtaata ataatatggg
    11101 gctgaacctc tatcgcgagt gacaatgctt ctttaccatt aactgccgta tggacagtat
    11161 gtccgagtat attgtttaag atgccctcgg taagcaggcg gctggacaat tcatcctcga
    11221 cgagtaaaac acgtaacgta gcagcatcaa tcgattcctc gggtctaggt gctggggtgc
    11281 tcgccatttt ctggaaagaa ggtggtgcaa ctgctggaag tttgagcatc tcaccccatt
    11341 gttgccagtt atgcataatt tgatcaataa attggccaaa cgactcggta tccagcccaa
    11401 tcttactacc taataacatt agatcagcgg tgcgtttact atgctcagat tctgggctga
    11461 tacctaaatc cgcaatttgc ttggcaaggt agagaagatg aacgatttga taagggcgcg
    11521 atccttcaga gaaacccgat tcatgaggtg attcgtggta atagatgggc tcaaccataa
    11581 tattgggaac accaaaatta atcaacatcg ctgcagttaa ttgattatgg tctgtttcca
    11641 aaaactgttg ttctagtaac ggaagtgata tgttggtatc ttgtttttct agaatctcag
    11701 tatatttacc aggataaatc gttgcgagcg ccaagcaacc aattcgtgtc attaaaccac
    11761 aggcaaacaa ctcatcaggg ggacaaactc gaactgattt accgagttct tgcatggcaa
    11821 ttgccattaa cagggaatgt gaccaaaatt gttgataatt aaaaccttta caaggcccac
    11881 cttgatattg gtcaatgagc gagaagccca aggctaattg tttgacaacg gtcaatccaa
    11941 cgcgagaaac tgcttctgtt accgaagtga tcggacgtgc agattggcta gcaaggtttg
    12001 ctttatgaat caatcgtccg gataaagcgg gatcggtttg aatgagttta gtgatttcac
    12061 ctaatgtggc gtcttctttt cggcaaactt caagcaatgc aagtgcaaca cctttggggc
    12121 tagggagcag ggcgctgata ttgagttgct taatatcggc gactttcata atgatagcct
    12181 aggggattca cagcgtcgat atcccaagat ttattcgaga gttgaagcat gtcattctta
    12241 tgcagcggct tactaaaata gaaaccttgc aatgtttcac aacctaaaaa ttttaatatt
    12301 tcagcttgat taagtgtttc gataccttct gcggtgatat gaaatcctaa attcttagat
    12361 agcgtgacga ttgatctaac aattgccaag ctgtctttat catcgagtaa accactcaca
    12421 aaactttgat ccactttgat gctgtcgatt ggtaagcgct ttagataatt catggaggag
    12481 tatcctgtac caaaatcatc gagtgcaatg tgaattttat tggctctaga ctagcagagt
    12541 taataatatc tgatgatttg ccatagttcg ttagca
//
LOCUS       DAOQLJ010000061        12525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1924_length_12525_cov_6.276022, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000061 DAOQLJ010000000
VERSION     DAOQLJ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12525
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1924_length_12525_cov_6.276022"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1089
                     /gene="plsX"
                     /locus_tag="PK522_05830"
     CDS             64..1089
                     /gene="plsX"
                     /locus_tag="PK522_05830"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNC41286.1"
                     /translation="MNITVAIDCMGGDHGPHVTVPSAAEYLLADPETDIILVGIPDAI
                     EAELKAIGVSLSTRLRIHAASEVVSMDEVPAFALRYKKNSSMRVAIDLVKNGEALACI
                     SAGNTGALLATSRFVLKTIPGIDRPALAVVLPTIKGHTYILDLGANVNCTAEHLLQFG
                     VMGATLVSSVENKKMPSIGLLNIGEEDIKGNEVVKQAAELLRTSGLNFYGNIEGDDIY
                     KGTTDVVVCDGFVGNVALKTSEGVAQMLATFLRQEFKRNVFTKLVGIVASPVINAFKQ
                     RVDHRRYNGASLLGLRGIVIKSHGSADKYAFTAAIRRATDEVRGGMLKHISERVAELQ
                     QGIGKNN"
     gene            1121..2077
                     /locus_tag="PK522_05835"
     CDS             1121..2077
                     /locus_tag="PK522_05835"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNC41287.1"
                     /translation="MYSKIAGTGSYLPEKILTNIELESIVDTSDEWIQTRTGIAQRHI
                     AADDQMASDLALAASRRALDAANLKAQDIDLIILATTTPDMIFPSTACILQEKLGITD
                     GPAFDVQAVCSGFIYALATADMFVSSGKSKNALVVGTEIYSRIIDWKDRSTCVLFGDG
                     AGAVVLSQSEQPGILSSHLHANGGYSKILAAPGSISGGKIQGTPFIAMEGNTVFKFAV
                     KALEEAAIESLAANNLQASDIDWFIPHQANIRIIKSTAHKLGIPMEKVVTTVDRHGNT
                     SAASVPLALDLAVRDGRIQPDHYVLLEGVGGGFTWGSILLQW"
     gene            2082..3032
                     /gene="fabD"
                     /locus_tag="PK522_05840"
     CDS             2082..3032
                     /gene="fabD"
                     /locus_tag="PK522_05840"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNC41288.1"
                     /translation="MIIAFTFPGQGSQSVGMLNGYSDLPIIKQTFDEGSEILGYDCFE
                     LAKHGPVEQLNLTIHTQPIMLMAGIAVYRAWKSLGGGSPDYLAGHSLGEYTALVAAEA
                     LAFRDAIKLVRYRAEVMQECVPNGMGAMAAIIGLEDDAVKAICNQIVGDNEKVVLEPA
                     NFNSPGQIVIAGHKAAVLEGMALAKSKGAKLVTLLPLSIPSHCSLMYPAAEKFQKLLE
                     QIDFYSPKIPVIHNADVQQHQQSSEIKKILMQQIHSPVHWVNTIQSFVSQGVTHIVEC
                     GPGKVLSGLTKRIDKTPTNYSLTDSESLQQAIEKLNQPAF"
     gene            3042..3794
                     /gene="fabG"
                     /locus_tag="PK522_05845"
     CDS             3042..3794
                     /gene="fabG"
                     /locus_tag="PK522_05845"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNC41289.1"
                     /translation="MEKKLLEGNIAFVTGASRGIGMAITLELAKQGAIVIGTATTQNG
                     ADGITRQLEEISAPGMGVVMNVNDVDQIGKAFEMTNKQFGDISILVNNAGITRDNLLV
                     RMKDEEWDEVMTTNLKSIFYLSKIALRAMMKARSGRIINISSVVGATGNPGQANYAAS
                     KAGMIGFTKSLAREVGSRNVTINCVAPGFIDTDMTSSLTTEQRLNLLKQIPLGRLGQS
                     ADIAAAVVFLASPAASYITGTTLHVNGGMFME"
     gene            3898..4143
                     /gene="acpP"
                     /locus_tag="PK522_05850"
     CDS             3898..4143
                     /gene="acpP"
                     /locus_tag="PK522_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNC41290.1"
                     /translation="MDNTDVEQRIRKIVAEQLGVNESDVKNESSFVNDLGADSLDTVE
                     LVMALEEEFDCEIPDEEAEKINTVQEAIDYVNSHSSN"
     gene            4179..5423
                     /gene="fabF"
                     /locus_tag="PK522_05855"
     CDS             4179..5423
                     /gene="fabF"
                     /locus_tag="PK522_05855"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNC41291.1"
                     /translation="MSKRRVVVTGLGIISPVGNNVSDAWQNIVSGNSGITRINRFDAS
                     NFSSQIAGEVKNFDITHYLSAKDARRMDIFIHYGMAAGIQAIRDAGIEKIDGLNPERI
                     GINIGSGIGGLTMIEDTDSAYHAGGPRKISPFFIPSTIINMIAGNLSIMFGYQGPNLA
                     IVTACTTSTHSIGSSARMIEYGDADVMVCGGAESCVTPLAIGGFSSARALSVRNSEPE
                     LASRPWDKGRDGFVLSEGAGVIVLEEIEHARRRNAKIYAELAGFGMSADAYHMTAPCD
                     DGSGASRCMLNGLNNAGINPDEVDYINAHGTSTQLGDIAETTAIKRCFADHARKLAIN
                     STKSMTGHLLGAAGGVEAIFSILSLYNQVVPPTINLIEPDPDCDLDYVPNTARDMKMN
                     VAVSNSFGFGGTNGTLVFRKFQ"
     gene            5436..6446
                     /gene="mltG"
                     /locus_tag="PK522_05860"
     CDS             5436..6446
                     /gene="mltG"
                     /locus_tag="PK522_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNC41292.1"
                     /translation="MPWQKQIVNKFKFLIIFFILGISLVTSWLYYLAHAAYVLPYKPY
                     EFSVEPGSSLKKIAVKLAEEKVIPSAWLFLLLSRITEQETAIKAGDYLLNRDISPLQL
                     LEYLAKGDSQRNEIRFIEGWTFAQIRETLNNHSGIKHDTLHLSEAEIATLLGANQKNL
                     EGLLFPDTYFFMKNSSDLGIIKRSYDKMQNHLQVAWSSKKESLPIKNEYEGLILASII
                     EKETGKDSDRELIAAVFINRLRLGMKLQTDPTVIYGLGDKFDGNLRKQDLVTDHAYNT
                     YTRLGLPPTPIAMPGLASIQAALNPAENNLLYFVAKGNGESHFSENLKEHNMAVQKYQ
                     KR"
     gene            6495..9110
                     /locus_tag="PK522_05865"
     CDS             6495..9110
                     /locus_tag="PK522_05865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC41293.1"
                     /translation="MQFSVIIPTLNESENIDTLLTRLFALQFSSENYEIIFVDDGSTD
                     HTQEKIKQWSSQYTNVHLIERKGKPDLTASILAGVSAAKSDVIAVMDADLSHLPEQLP
                     LILEPVLTDRYDVAVGSRYVPGGSTKGWPLYRQWLSRAGGWLARPICDVNDATSGFFA
                     FKRNLISNIPEMARGYKILLELLVSNQEKIRITEVPICFHNRTQGSSKLSISHQVIFL
                     HRLIMLAGGIVSVGTASRFAMVGFLGVFVDAAVFHWLISSGAGLALAHITSFLAAVTV
                     NYTLNSKWSFRAHNGGRLDWYQFSRYLIVGLFALLLRGGVLALLVYTWDIPNGWAIFP
                     AIAATAIVNYLGSAFYVFPEKRTTLSLDTRWRVAAVGVVLFVILLRLVYIGLAQLLPD
                     ETYYWQYSQHMDLSFFDHPPLVAWLIWIGTAIAGQNEFGVRLGAFICGLVTLIYLYGF
                     ARNLYNKSVGMRAVMLFSVLPFGFASGLIMTPDAPLIAAWAATLFYMERALIAGQKSA
                     WLGMGIAFGLGLLSKYTLGLLGIAALVFVILDSTARRWMIRPYPYLSALLAFVLFLPV
                     VVWNAENNWVSFLFQTERIGGGGHVFSVHYLILYIAIILTPIGLFSAAHALVRAPYDK
                     NSDFERRRRLFIYVFTGIPLAIFLGLSTYDTPRFHWTGPLWLAIIPAMAWVMGQNTLD
                     KFFNGLRNAWQSTIVVSLLAYAFILHYVVLGFPGVPYQFLTEHYFWKEATIEVEQIAE
                     EIKTETGQTPIVVGMSKWSVASALSFYNQNGNRMDIRSRNMLGDTGAMYEYWFPSEPP
                     TDRPILLVGMNRYALERSRTGNQFDHMLNHPSSIQHRVVTRHGIPVRPLYYRIAKGYL
                     GSATPYFAKNATSSP"
     gene            9220..10338
                     /locus_tag="PK522_05870"
     CDS             9220..10338
                     /locus_tag="PK522_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNC41294.1"
                     /translation="MILVLKPNTLLESLEYKQLLAHLGNLSGISTRIHSETGIEQTLT
                     EIYLIGNTKVLSIEDIQCFPCVDRVVRVSEEYRVLGRHQNDDRPTYFEYNGVRFGQDT
                     LNVFAGLCAVDTLEHVELMMKALRDHGQVCTRMGAYKPRTSPYSFQGHGKSCLPYVFD
                     LAGKYGIKVIAMEVTHESHVEEIREALYQTGSSAGVMLQIGTRNTQNFELLKIVGRQQ
                     DFPVLIKRGFGITLDESLNAAEYLASEGNRKVVFGLRGMKTNMGDPYRNFVDFAHVPV
                     VKRLTRMPVCIDPSHSVGMRSIAPDRILDIMHVTAQGVIAGANMVLADFHPVPSKALV
                     DGPQALLIKELPFFLEDIQIAREAYEKRVRLATRYLEV"
     gene            10423..11274
                     /locus_tag="PK522_05875"
     CDS             10423..11274
                     /locus_tag="PK522_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNC41295.1"
                     /translation="MGLVVADTQGNRSDSLNLANRSNNIDFNAKRIISLSPAITELVF
                     SAGAGGKLVGVSRFSNFPSEAQLIPEIGDAYNLDLEKIIEISPELIIAWKQASIHKDL
                     DRLNNLGFQILEIEIKSLDDIPNLIRLIGKLAETAERAALAASQYEKTLLQMKQNYDN
                     RTLISVLQLIWLQPLITFGKDHLINEILGVCGGENVANFYPGSTFALATENIVAANPE
                     VIFDSTATMPEVRDLFKKLSSISAVKNDYIYFIPPDLIFRETVRALDAIQIACDYFDE
                     VREKMYQ"
     gene            11373..12344
                     /gene="ispH"
                     /locus_tag="PK522_05880"
                     /gene_synonym="lytB"
     CDS             11373..12344
                     /gene="ispH"
                     /locus_tag="PK522_05880"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNC41296.1"
                     /translation="MIKVLLANPRGFCAGVDRAIEIVERALNMHGAPIYVRHEVVHNR
                     FVVEDLEKKGAVFVENLEEVPQESILIFSAHGVSHAVRREAAARKLKIFDATCPLVTK
                     VHVEVAKMRKEGKEIVMIGHHGHPEVEGTMGQVEGNDKGMYLVETEDDVANLQVRDEK
                     NLTYVTQTTLSVDDAARVIDALKKRFPAITGPKKDDICYATQNRQDAVKKMVRNCDLV
                     IVVGSPNSSNSNRLCEVARNANVEAYMVDRAEQLQESWLIGKKCIGITAGASAPEILV
                     QQVLARLKQIGSNQTGQEVSVEELSGVVESVVFPLPKAEAISLEKHF"
     gene            complement(12319..>12525)
                     /locus_tag="PK522_05885"
     CDS             complement(12319..>12525)
                     /locus_tag="PK522_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41297.1"
                     /translation="TIGRHPELDNLFINSGHFRYGVTMAPGSAEILLNEIMGKPQPFD
                     ISPYQRGWHLPEKFNFSKNASLMK"
BASE COUNT         3629 a         2165 c         2911 g         3820 t
ORIGIN      
        1 tatgctcaaa atagtcaaaa acaaacaaat agcattgaat agtattttga taatcaacgc
       61 aaagtgaaca ttactgtagc gattgattgc atgggaggcg atcatggtcc ccatgttaca
      121 gtaccctctg cagcagaata tttattagct gatccagaaa cagacattat attggtcggt
      181 atacctgatg cgatcgaagc tgagttaaaa gccattggtg tatcgttaag tactcgttta
      241 agaatccatg cggcaagtga agtagttagt atggatgaag tgccggcatt cgctttacgc
      301 tacaaaaaaa attcgtcgat gcgagtggcg atcgatttgg tcaaaaatgg tgaagcttta
      361 gcttgtatta gtgctggtaa cacgggtgcg ttattagcaa cctcgcgttt tgtattaaaa
      421 acaattccag gtattgatcg gccagcgcta gcagtcgttt taccgacgat aaaggggcat
      481 acttatattt tagatttagg ggctaatgtc aattgtaccg ctgagcattt gctacaattt
      541 ggcgtaatgg gtgcgacgct agtatcttct gttgagaata aaaagatgcc cagtatcggt
      601 ttgcttaata tcggtgaaga agatattaaa ggtaatgaag ttgttaaaca agccgcggag
      661 ctattaagaa caagtggttt aaacttctac ggtaatatcg agggtgatga tatttataag
      721 ggaactaccg atgtggtcgt gtgtgatggc tttgtgggga atgtggcttt aaaaacttct
      781 gaaggggtgg cgcaaatgtt ggccactttt ttgcgccagg agtttaaacg aaatgttttt
      841 acaaaacttg tcggtatagt tgcatcacca gtcatcaatg cttttaagca gcgagttgat
      901 catcgtcgat ataatggggc tagtttgttg ggtcttaggg ggattgtgat taagagtcat
      961 ggttctgctg ataaatatgc ttttactgca gccattaggc gagcaactga tgaagtacgt
     1021 ggaggtatgc taaagcatat tagcgagcga gtggctgaat tacagcaagg cattggaaaa
     1081 aataattaaa ttaagctctt attgagtgat aaaaatggca atgtattcaa aaatcgctgg
     1141 aactggtagc tatttaccag agaaaattct gactaatatt gagttagaaa gtatcgtgga
     1201 tacaagtgat gagtggatac aaactcgaac tggcattgct cagcgacata ttgcagcaga
     1261 tgatcaaatg gcaagcgacc ttgctttagc ggccagccgt cgagcactag atgcagcaaa
     1321 tcttaaagca caggatatcg atctgatcat ccttgctacg actaccccag atatgatttt
     1381 cccaagtacg gcctgtattt tgcaagaaaa actcggtatt accgatggtc ctgcatttga
     1441 tgtacaagct gtttgcagtg gttttatata tgcactggca acagcggata tgtttgttag
     1501 ctcgggaaaa agtaagaatg cccttgtggt tggtacggag atatattcgc gtattattga
     1561 ttggaaagat cggagtacat gcgtattgtt tggtgatggt gccggtgcgg ttgtgttgtc
     1621 gcaaagtgaa caaccgggga tactctcaag ccatttacac gcgaatgggg gttacagcaa
     1681 gatactagct gcacccggca gtatttctgg aggaaagatt caaggaacgc cttttattgc
     1741 aatggaaggg aacacggtat tcaagtttgc ggttaaagca ctggaagaag cggcaattga
     1801 atctttggca gctaataatt tgcaagcctc tgatatcgat tggtttatac cgcatcaagc
     1861 taatattcgt atcattaaat cgacagctca caagcttgga ataccgatgg agaaagtagt
     1921 cacaacggta gatcgacatg gtaatacttc agctgcatcc gtgccccttg cattagatct
     1981 tgcagtccgt gatggacgta ttcagccaga tcactatgtt cttctagaag gagtaggtgg
     2041 cggttttaca tggggatcta tattgcttca gtggtgaaaa aatgattatt gcttttacgt
     2101 ttcctggaca aggttcccaa tcggttggca tgctgaatgg ctacagtgat ctgccaatta
     2161 ttaaacagac atttgatgaa ggctctgaaa ttctaggcta tgactgtttt gaattggcaa
     2221 aacatggtcc agtagaacaa cttaatttga cgattcacac tcaaccaatc atgttgatgg
     2281 cgggtatcgc agtttatcga gcctggaaat ctttaggggg gggaagtcca gattacttag
     2341 caggacatag cctgggcgaa tatacggcat tagtggcagc agaagcgtta gcttttagag
     2401 atgccattaa gttagtgcga taccgtgcag aggtcatgca ggaatgcgtg ccaaacggaa
     2461 tgggtgcaat ggccgctata attggattag aggatgatgc tgtcaaggca atttgtaatc
     2521 aaattgttgg tgataatgag aaggttgtac ttgagcctgc caattttaat tcgcctggtc
     2581 aaatcgtgat cgctggacat aaagctgcgg tattagaagg aatggcgcta gctaaatcga
     2641 aaggggcaaa attagttacc ctgcttcctt tgagcatccc ctcacattgt tctctgatgt
     2701 atcctgccgc tgaaaaattt cagaaattgc ttgagcaaat tgatttttat agtccgaaaa
     2761 taccagttat acataatgct gatgtgcaac aacatcagca gagtagtgaa atcaaaaaaa
     2821 tattaatgca gcaaatccat agtccggtgc actgggttaa taccattcaa tcttttgttt
     2881 ctcagggagt gacgcatatt gttgaatgtg ggcctggaaa agtactctct ggccttacaa
     2941 aacgcattga taaaaccccg accaattatt cactaaccga tagtgaatcg ctccagcaag
     3001 cgattgagaa attgaatcaa cctgcttttt gataagaatg tatggaaaaa aaattgcttg
     3061 aaggaaatat tgcttttgtg actggagcaa gccgtggaat aggtatggct attacgttgg
     3121 agcttgctaa acaaggtgca attgtcattg gaacagcaac aacccagaat ggtgctgatg
     3181 gtataacgcg ccaattagaa gagatcagtg cccctggaat gggtgttgtc atgaatgtca
     3241 atgatgttga tcaaattggt aaggcatttg aaatgaccaa taaacaattt ggcgatatct
     3301 caatattggt taacaatgcc ggtatcacgc gagataattt actggttcga atgaaagatg
     3361 aagagtggga tgaagtcatg acaaccaatc ttaaatcaat tttttatctt tccaaaatag
     3421 cactccgagc catgatgaaa gcgcgttctg gacgaattat taatatttct tctgttgtcg
     3481 gagcgactgg taatccgggg caggctaatt atgcagcatc aaaggctggt atgattggtt
     3541 ttacaaaatc tctagcgaga gaggtcggaa gtagaaatgt tacgatcaac tgtgtagctc
     3601 ctggttttat tgacacagac atgacaagtt cgttaacgac tgagcagcgc cttaatttac
     3661 ttaagcaaat acctctgggt cggctgggtc agtcagcgga tattgcagca gcggtggtgt
     3721 ttttagcttc gcctgctgca agctatataa ctggaactac gttgcatgtt aatggcggaa
     3781 tgtttatgga gtaatcacga aatcttattg ataaacctga tacgattagg tttggttttt
     3841 taaaaaacga aattttgtta aaatcactta gttaattacc tcacaaaagg ataccagatg
     3901 gataatacag atgtagaaca aagaattaga aaaattgttg ctgagcagct cggtgtgaac
     3961 gaaagtgatg taaaaaacga atcctctttt gttaacgact taggcgctga ttccctcgat
     4021 acagttgaac tggtgatggc acttgaagaa gaatttgatt gtgaaattcc agatgaagag
     4081 gctgaaaaaa tcaacaccgt acaagaagca attgattacg taaattcaca ttcctccaat
     4141 tgatattatt tatatagctt tttctttagg agatacattt gtcaaaacgc agggtggtag
     4201 taacggggtt aggtataata tcgcctgtcg gcaataatgt atcggatgct tggcaaaata
     4261 tcgtttcagg gaattcaggt atcacgcgta taaatcgttt tgacgcttca aatttttctt
     4321 cccaaattgc aggtgaagtc aaaaattttg atattactca ttatttatca gcgaaagatg
     4381 cacgtaggat ggatattttt atacactatg gcatggctgc tggtattcag gcgattagag
     4441 atgcaggtat tgaaaaaatc gatgggttaa atccagagcg tatcggtatt aatattggtt
     4501 ctggtattgg tggacttaca atgattgagg acactgatag tgcctaccat gctggaggcc
     4561 cccgtaaaat atcgcctttt tttataccca gtacgattat taatatgata gcgggtaatt
     4621 tatcgattat gtttggttat caaggaccga acttggcgat tgtaactgct tgtactactt
     4681 ctactcatag tattggtagt tctgcacgaa tgattgaata tggtgatgcg gatgttatgg
     4741 tatgtggtgg cgctgaatct tgcgtaactc ctcttgcaat cggtggattc tcatcggcac
     4801 gtgctttgtc agtcagaaat tcagaacccg aattagcaag tcgtccctgg gataaaggta
     4861 gagatggttt cgtattgagt gagggcgctg gcgtaatcgt actagaagaa atagagcatg
     4921 ctaggcgacg taacgctaaa atttatgctg agttagctgg atttggaatg agcgcagatg
     4981 cctatcatat gactgctccg tgtgatgatg gtagtggtgc ttctcgatgt atgctaaatg
     5041 ggctcaataa cgcgggtatc aatccagatg aagttgatta tattaatgcc catggtactt
     5101 ctactcaatt aggtgatatt gcggaaacga ctgcaatcaa acgttgcttt gctgatcacg
     5161 ctaggaaatt agcgatcaat tctactaaat caatgactgg ccacttactt ggcgcagcag
     5221 ggggagtaga agctattttc tcgattctgt cgctatataa tcaggttgta cctccaacta
     5281 taaatctaat cgaaccagat ccagattgcg atctagacta tgttcctaac acagctcgag
     5341 atatgaaaat gaatgttgca gtttctaact cgtttgggtt tggcggaaca aatggtacat
     5401 tggtatttag aaaatttcag tagattactt tgcatatgcc atggcagaag caaatcgtta
     5461 ataaattcaa gtttctcatc atttttttta ttcttggtat ttctcttgtt actagctggc
     5521 tctattatct agcacatgca gcatatgtat tgccctataa accttatgaa ttttcagttg
     5581 aacccggcag cagtttaaaa aaaatcgcgg taaagcttgc tgaagaaaag gtaataccga
     5641 gtgcttggtt attcttgcta ttatcaagaa taacagagca agaaacagcg attaaagcgg
     5701 gcgactattt gctaaatagg gacatttcac cacttcagct cctggaatac cttgccaaag
     5761 gcgatagcca acgtaatgaa attaggttta tcgaagggtg gactttcgct caaattcggg
     5821 aaacactcaa taatcactca gggattaagc atgatacgtt gcacttgagt gaagcagaga
     5881 ttgcaacgtt acttggcgca aatcaaaaaa acttagaagg cttgttattt cccgatactt
     5941 atttctttat gaaaaatagt agtgatctag gtattataaa acgatcctat gataagatgc
     6001 agaatcatct ccaggttgct tggtcgtcaa aaaaagaatc gctaccgatt aaaaatgaat
     6061 atgagggctt gattcttgct tcaatcatcg aaaaagaaac aggaaaagat agtgatcgag
     6121 aattgatagc agcagtattt atcaatcgcc tgcgcctcgg gatgaaacta caaaccgatc
     6181 caacagtaat atatgggtta ggtgacaagt ttgatggaaa tttgcgaaag caggatctag
     6241 ttactgacca tgcctacaac acttatactc gattaggttt acccccaact cccattgcaa
     6301 tgcctggtct tgcttctatc caagcagcgc ttaatccggc tgaaaataat ttgctctatt
     6361 tcgtggctaa agggaatggc gaatcacatt tctcagaaaa cctaaaagaa cataatatgg
     6421 ctgtacaaaa ataccagaag cgataattga ttgtttctta tgaataatat aataaataag
     6481 gatgaataaa cctgatgcaa ttctcggtta ttattcctac acttaacgag tcagaaaata
     6541 ttgatacatt attgacgcgt ttgtttgcac tccaattttc ttcagaaaac tatgaaatta
     6601 tttttgtgga tgatggctca accgatcata cacaagaaaa aattaagcaa tggtcatcac
     6661 aatatactaa tgttcatctt atagaaagaa aaggaaagcc tgatttaacc gcctcgattc
     6721 ttgcaggggt atccgcagca aaaagtgatg tgattgctgt tatggatgca gatttgagtc
     6781 acctgcctga gcaactaccg ttaattttag agccagtatt aactgatcga tatgatgttg
     6841 cggttggaag tcgatatgtc ccaggaggaa gtaccaaggg atggccttta tatcgacaat
     6901 ggctttctcg tgctggcggt tggttagcgc gtcctatttg tgacgttaat gatgcaacgt
     6961 ctggtttttt tgcatttaaa cgtaacttga tcagtaatat tccagagatg gcgcgtggct
     7021 ataaaattct tctggaatta ttagtatcaa atcaagaaaa aattcgaatt acggaagtac
     7081 cgatttgttt tcataacaga acacaaggta gctctaaact ttcaatttct catcaagtca
     7141 tttttttaca tcgactgatt atgttagccg gtggaattgt atctgttgga actgcaagtc
     7201 gctttgcaat ggtgggattt ttaggagtgt ttgttgatgc ggcggttttt cactggctga
     7261 taagttcagg cgctgggtta gctttggccc atatcactag ctttctagca gctgtgactg
     7321 ttaattatac attgaattct aagtggtcat tccgagcaca taacggaggt cgattagatt
     7381 ggtatcagtt tagtcgttac ctgatcgttg gactttttgc tttgttgcta aggggaggag
     7441 tacttgcgct ccttgtgtat acctgggata tacccaatgg atgggcaata tttcctgcaa
     7501 ttgctgcgac tgcaattgta aattatcttg gttcggcttt ttatgttttt cctgaaaaaa
     7561 gaacaacact atcactggat actcgctgga gagttgcagc agtcggggtt gttctttttg
     7621 tgatattgtt gcgccttgtt tatattggat tggctcaatt gctgccggat gagacttact
     7681 attggcaata ttcccagcat atggatctaa gtttttttga tcatccaccc ttagtcgcat
     7741 ggttaatttg gattggaact gctatagcag gtcagaatga atttggagtg aggttgggtg
     7801 cgttcatatg tggtttagtg acgctcatat atctctatgg tttcgcacgt aacctgtata
     7861 acaaatcagt tggtatgcga gccgtaatgc ttttctctgt tttaccattt ggctttgcat
     7921 ccggtttaat tatgacgcca gatgcacctc tgattgctgc ctgggctgct actttatttt
     7981 atatggagcg ggcattgatt gcgggtcaga aaagtgcatg gcttggcatg ggaattgctt
     8041 ttggcttggg tttgttatcg aaatacacgc tcgggttact cggtattgcc gcgttagttt
     8101 ttgttatttt ggattccacc gcgcgccgtt ggatgattcg gccatatccc tatttatctg
     8161 cgctgctcgc ttttgttctc tttttgcctg tggtcgtatg gaatgctgag aacaactggg
     8221 tttcgttttt gtttcaaact gaaagaatcg ggggcggcgg ccatgtgttt tctgttcatt
     8281 acctaatttt atatattgca attatattga caccgatagg tttgttttca gctgctcatg
     8341 cattagtccg tgctccatat gacaaaaata gtgattttga gcgtaggcgt cggttattta
     8401 tttatgtttt tacaggtatt ccactcgcca tcttcttagg tcttagcacc tatgatacgc
     8461 ctcgatttca ctggacgggg ccgctctggc tagcaataat tcctgcgatg gcctgggtga
     8521 tgggacaaaa tacacttgat aaatttttta atggcctacg aaatgcatgg caatctacca
     8581 tcgttgtatc attgctggcc tacgctttca ttttgcatta tgtcgtactg ggttttcctg
     8641 gggtacccta ccaattctta acagaacatt atttctggaa agaagcaacg attgaagttg
     8701 agcaaattgc cgaagaaatc aaaactgaga ctgggcaaac accaattgta gtagggatga
     8761 gtaaatggtc ggtcgcgagc gcgctatctt tctataatca aaacggcaat aggatggata
     8821 tacgttcacg taacatgctt ggggatactg gagcaatgta tgaatactgg tttccaagcg
     8881 agccacccac agatcgccct atactattgg tgggaatgaa tcgctatgca cttgagcgaa
     8941 gtcggactgg aaatcagttt gatcatatgc taaaccatcc tagttcaata caacaccgtg
     9001 tggttactcg gcatggtatt ccggtaagac ctttgtatta tcgaattgct aaaggttatc
     9061 tcggtagcgc aaccccatat tttgcaaaga atgcaactag ttctccataa aataaacgtt
     9121 ttcagcttaa tagaaatggt aataaactat tcctagatct aaagctagct ggaagcgatt
     9181 taccttgaag attactattt attgctatta ggcattttta tgattcttgt tttaaagcca
     9241 aatactttac ttgagagctt ggaatataag cagctacttg cgcatctggg aaatttgtct
     9301 ggtatttcta ctcgaattca ttctgaaact ggtattgaac aaacgctaac agagatttac
     9361 ttgatcggta ataccaaagt attatcgata gaagacattc aatgtttccc ttgtgtcgat
     9421 cgtgtggtca gggtctcaga agaatatcgc gtgttaggga ggcaccaaaa tgatgatcgt
     9481 ccaacctatt ttgagtataa cggggtgcgc tttggtcaag acacattgaa tgtgtttgca
     9541 gggctttgtg cagttgatac gttagagcat gtagagctca tgatgaaagc gttgagagac
     9601 catggtcagg tatgtactag aatgggagcc tataaaccac gaacaagtcc ttactcattt
     9661 caaggtcatg gtaaatcttg tttgccttat gtgttcgatc ttgctggtaa gtatggtatt
     9721 aaagttatcg caatggaagt gacgcacgag tctcatgttg aggaaattcg tgaagcttta
     9781 tatcagacag gaagttctgc aggggtaatg ctgcagattg gaacgcgtaa tacacaaaat
     9841 tttgaattgt tgaagattgt tggtcgtcag caggattttc cggttcttat taaacgtggt
     9901 tttggaatta cattggacga atcactaaat gcagccgagt atcttgcttc cgaaggtaat
     9961 cgaaaagtgg tttttggttt gcgtggaatg aaaacgaata tgggtgatcc ataccgtaat
    10021 tttgtcgatt ttgcacatgt tcctgttgtc aaacgtttga cgagaatgcc ggtttgtatt
    10081 gatccatcac attcagtcgg tatgcgttca attgcgccgg atagaatttt ggatattatg
    10141 catgtgactg cacaaggtgt tattgctggc gctaatatgg tactggctga ttttcatcct
    10201 gttccaagta aagcgctcgt tgatggacct caagcattat tgatcaaaga gttgccgttt
    10261 ttcctagagg atattcaaat cgcgcgtgaa gcttatgaaa aaagggtaag actcgctacc
    10321 cgttatctgg aagtgtaatt gaattgatct ttatcggtgt ataactttga gtttagctga
    10381 ttgagaacat actcaatttg tttacttctc ctattttttg ctgtgggact tgttgtcgct
    10441 gatactcaag gaaacagaag cgattcccta aatctcgcta accgttctaa taatattgat
    10501 tttaatgcta agagaattat ctcactttct cccgcaatta ctgaattagt tttttcagct
    10561 ggggctggag gtaagctagt aggcgtaagt agatttagta attttcctag cgaagctcaa
    10621 ttgattcctg aaatcgggga tgcatataac ctcgatcttg agaaaattat cgaaattagc
    10681 cctgaactca ttattgcttg gaagcaggct tcgatccaca aagatcttga tcgattgaat
    10741 aatcttggtt ttcagatact tgagattgag atcaagagcc tggatgatat acccaattta
    10801 ataaggttaa ttggtaaact ggcagagaca gcagaacgcg cagcattagc ggcgagtcaa
    10861 tatgagaaaa ctttgcttca aatgaagcag aattatgata atagaacgct aataagtgtc
    10921 cttcaattaa tttggttaca accgctaata acgtttggaa aggatcatct aattaacgag
    10981 atattagggg tatgtggtgg ggaaaatgta gctaattttt atccaggtag tacatttgca
    11041 ctcgctacag aaaatattgt agcagcaaat cccgaagtga tttttgatag tacagctact
    11101 atgccagagg tacgggatct ttttaaaaag ctttcatcga ttagtgcagt gaaaaacgat
    11161 tatatctact ttattccacc tgatttaatt tttagggaaa ctgtacgagc tctcgatgcc
    11221 attcaaatag cttgtgatta ttttgatgaa gttcgagaaa aaatgtatca gtgaaggcaa
    11281 cgtataatat cttataatgc gagtaatgtt cataataaat aaaaaagtta gtttttctaa
    11341 ctaaagctaa atgcttttat ggagaagaga aaatgattaa agttttgcta gctaatccca
    11401 gaggattttg cgcaggcgtt gatcgcgcga tagagattgt agaacgtgcg cttaatatgc
    11461 atggtgctcc aatttatgtg aggcatgaag tagtgcataa ccgctttgtt gttgaggatt
    11521 tggagaaaaa aggcgcagtg tttgttgaga atttagaaga agtgccacaa gagagtattt
    11581 tgatctttag cgcgcatggt gtttcacatg cggttcgtcg tgaagcagca gcacgaaaac
    11641 ttaagatttt tgacgcaact tgcccactcg ttacaaaagt tcatgtcgaa gtagcgaaaa
    11701 tgcgtaaaga aggtaaagag attgttatga ttgggcatca tgggcaccct gaggtagaag
    11761 gaacgatggg gcaagtggaa ggaaatgata aaggaatgta tcttgtggaa acagaagatg
    11821 atgttgccaa ccttcaagtt agagacgaaa agaatttaac ttatgtgact cagaccactt
    11881 tatctgttga tgatgcagct cgtgtaattg atgctttgaa gaaaagattt ccagcgatta
    11941 ctggcccaaa gaaagatgat atttgttacg cgactcagaa tcgccaagat gcagttaaaa
    12001 aaatggtacg gaattgtgat ttagtgattg tggtcggttc tcccaatagc tcaaactcta
    12061 atcgcttgtg tgaagttgcg cggaatgcca atgttgaagc atatatggtt gatcgagcgg
    12121 aacagcttca agagtcatgg cttataggaa agaaatgtat aggtattact gcgggggcat
    12181 ctgctccaga aatattggtt cagcaagttt tggctaggct taagcagatt ggctctaatc
    12241 aaacgggtca ggaagtgagt gtcgaagagc taagtggtgt tgttgaatca gttgtttttc
    12301 cgctacctaa agcggaggct atttcattag agaagcattt ttagaaaaat tgaatttttc
    12361 aggcagatgc catccccttt gataagggga aatatcaaag ggttgtggtt ttcccattat
    12421 ttcgtttaag aggatttctg cacttcctgg agccatcgtt actccatagc gaaaatgacc
    12481 gctgttgata aaaaggttat ccaattcagg atgtcgccct atggt
//
LOCUS       DAOQLJ010000062        12514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1928_length_12514_cov_6.062846, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000062 DAOQLJ010000000
VERSION     DAOQLJ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12514
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1928_length_12514_cov_6.062846"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="PK522_05890"
     CDS             complement(<1..1009)
                     /locus_tag="PK522_05890"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNC41298.1"
                     /translation="MQPIKKSSKLENVCYDIRGPVLDRARQMEEEGYHIIKLNIGNPA
                     SFGFEAPEEILQDVIRNLSAASGYCDSKGLFAARKAIMHYTQEKQIANVQMEDIYVGN
                     GVSELIMLTMQALLENNDEVLIPAPDYPLWTAAVSLAGGTARHYVCDEQSDWFPDLDD
                     IRTKITDRTRAIVIINPNNPTGSLYPKELLLEIIEIARQNRLIVYADEIYDKILYEQA
                     THTSVASLADDVLFITFNGLSKNYRAAGFRSGWAVVSGNKHDAQDYIAGLNMLASMRL
                     CANVPAQFGIQTALGGYQSIHDLTMPTGRLKRQRDLAWQMLTDIPGVSCYKPQSALYL
                     FP"
     gene            1203..1571
                     /locus_tag="PK522_05895"
     CDS             1203..1571
                     /locus_tag="PK522_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HNC41299.1"
                     /translation="MKFHLSNIEGINTFSGYGVGYVVINQVRYDRNLVVFPDRIIENW
                     CIRNSGRFAIDDFDEILTYQPEIILLGTGETQRFPSPQLIKAMLNARIGLEVMDTFSA
                     CRTYNILSTEGRRVAAALLV"
     gene            1637..1921
                     /locus_tag="PK522_05900"
     CDS             1637..1921
                     /locus_tag="PK522_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41300.1"
                     /translation="MGALVFYTFVFFIGYFVIHGFNLLIGRKLLNRKITALVIVCLIA
                     LMHGYKILTSPLPAQQDADAFQALGYYVMFPILVIVTACVFFGLQDKEDE"
     assembly_gap    1947..2046
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2123..3205
                     /gene="hisC"
                     /locus_tag="PK522_05905"
     CDS             2123..3205
                     /gene="hisC"
                     /locus_tag="PK522_05905"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNC41301.1"
                     /translation="MTHLLRNVIRPEIFELSAYHVPPAKGMIKLDAMENPYTLPLTLR
                     AEIAQLAENATINRYPDPTASALKSVLRKTLSVPDDMSILLGNGSDEIIQMIALAAAK
                     PGAVLLGVDPGFAMFRIIATFSGMSYVGVPLNEDFSLNLEAMRTAITRYQPAIIFLAY
                     PNNPTGNLFDIQVIHQIIQEAPGIVIIDEAYHAFAGVSFMDQLAQFPNLLLMRTLSKL
                     GLAGLRLGLLIGRSEWLEQFEKLRLPYNIGVMTQLIAEKVLQHYEDLLKQTAAIKTER
                     SKLLSELSGLSGITTFPSEANFILFRIKRASEVYALLKQKGVLIKNLDGAHPLLKDCL
                     RVTVGTPHENDQFYHALLQVLEDTNC"
     gene            3244..3831
                     /gene="hisB"
                     /locus_tag="PK522_05910"
     CDS             3244..3831
                     /gene="hisB"
                     /locus_tag="PK522_05910"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNC41302.1"
                     /translation="MRQIKVTRNTLETQISVEVNLDGSGHSKLNTGIPFLDHMLDQIA
                     RHGMIDITVEAKGDLHIDAHHTVEDIGITLGQALAKAWGDKKGVRRYGHAYVPLDEAL
                     SRVVLDLSGRPGLGYRVEFVRARIGEFDVDLIHEFFQGLVNHAMITLHIDNLFGDNAH
                     HQAETVFKAFGRALRMAIETDSRAINLIPSTKGSL"
     gene            3863..4513
                     /gene="hisH"
                     /locus_tag="PK522_05915"
     CDS             3863..4513
                     /gene="hisH"
                     /locus_tag="PK522_05915"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNC41303.1"
                     /translation="MTAIAIVDYGMGNLRSVSKALERVAPYATVQVTSDPKIVDKASH
                     IVVPGQGAMPHCMQELDKFGLKDVVIDAARNKPFLGICIGLQMLFDESEEGNVRGLGV
                     FPGKVVHFSAGGEADVQGGKLKIPHMGWNQVYQTMNHPLWDGIRSDARFYFVHSYYVK
                     SEDPNLSAAYSLYPTPFTCAIAKDNIFAVQFHPEKSHIAGLTLLSNFAKWEPHSSH"
     gene            4575..5321
                     /gene="hisA"
                     /locus_tag="PK522_05920"
     CDS             4575..5321
                     /gene="hisA"
                     /locus_tag="PK522_05920"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNC41304.1"
                     /translation="MLIIPAIDMKDGQCVRLKQGDMEAATVFSENPDEMALQWLNQGA
                     RRLHLVDLNGAFAGKPKNESVIRSIVKALNGNIPIQLGGGIRDLETIERYLDQGITYV
                     IIGTAAVKIPGFLHDACYAFPGQIMVGLDAKNGKVAVDGWSKVTGHDVVDLAKKFEDY
                     GVEAIIYTDIGRDGMLSGVNIEATVALARELTVPVVASGGISNLEDIKALCKIEAEGV
                     MGAITGRALYEGTLDFKQAQDLADKLSVTA"
     gene            5408..6184
                     /gene="hisF"
                     /locus_tag="PK522_05925"
     CDS             5408..6184
                     /gene="hisF"
                     /locus_tag="PK522_05925"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNC41305.1"
                     /translation="MGLAKRIIPCLDVNNGRVVKGVNFVSLKDAGDPVEIARRYDEQG
                     ADELVFLDITASSDNRDLILHIVEDVASQVFIPLTVGGGVRKVEDVRRLLNAGADKVS
                     INTSAVLNPQLVADAAGYYGAQCIVVAIDAKQVGEQGSQVPRWEVFTHGGRNATGLDA
                     IEWAKKMQALGAGEILLTSMDRDGTRHGFDLALTRTLSDAIDLPVIASGGVGSLNHLV
                     DGILQGHADAVLAASIFHYGQHTILEAKEFLSQHGIEVRL"
     gene            6228..6614
                     /gene="hisI"
                     /locus_tag="PK522_05930"
     CDS             6228..6614
                     /gene="hisI"
                     /locus_tag="PK522_05930"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNC41306.1"
                     /translation="MPASWVDKINWTIDGLVPVIAQEEATGTILMFAWMNKEAVKLTV
                     ETKVVVYWSRSKQRLWRKGEESGHTQKVKAIYLDCDDDVLLIQVEQMGGIACHTGRKS
                     CFFNKLEGDQWVVNAPIIKDPSEIYQ"
     gene            6615..6944
                     /locus_tag="PK522_05935"
     CDS             6615..6944
                     /locus_tag="PK522_05935"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HNC41307.1"
                     /translation="MSHASILHHLATVIDDRRTADPSTSYVAKLLNSGQDKILKKVAE
                     EAAETIMASKDGDTTHVVYEVADLWFHCLILLAHHGLRPEDVLAELARREGLSGIEEK
                     RLRNTEK"
     gene            6945..7298
                     /locus_tag="PK522_05940"
     CDS             6945..7298
                     /locus_tag="PK522_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNC41308.1"
                     /translation="MSDCIFCKIVRKEIPAKIIYEDQHCLAFHDINPAAPVHFLIIPK
                     LHIASLYEVDDTHQLILGKMLGLVPKLAKQEGCEHGFRTIINTGKVGGQEVFHLHIHV
                     IGSKNVLPAMIHRSQ"
     gene            7323..7562
                     /gene="tatA"
                     /locus_tag="PK522_05945"
     CDS             7323..7562
                     /gene="tatA"
                     /locus_tag="PK522_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HNC41309.1"
                     /translation="MGTFSIWHWLVVLAIVVLVFGTKKLRNLGSDLGNAVKGFKEGMK
                     GSEGEPMESPANTQQQIANRSTEDVSIKEKNETKL"
     gene            7682..8071
                     /gene="tatB"
                     /locus_tag="PK522_05950"
     CDS             7682..8071
                     /gene="tatB"
                     /locus_tag="PK522_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HNC41310.1"
                     /translation="MFDISFAELLVISVVALVVIGPERLPRVARTLGFLLGRARRYVE
                     TVKNDIRNEIELDELRRIKDTVQDTAQSIEKSVKQEIQQIQASVQPTASDSHDKKIKD
                     TSPVNNNHAGHEATPIASQSISNSKGD"
     gene            8155..8937
                     /gene="tatC"
                     /locus_tag="PK522_05955"
     CDS             8155..8937
                     /gene="tatC"
                     /locus_tag="PK522_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNC41311.1"
                     /translation="MDEEGSFISHLVELRTRLIRIITVLVVGFIPCAYFARELYTLLA
                     QPLLEKLPQGGQMIATAVATPFFVPMKVALMTAFLLTLPHTLYQIWAFVAPGLYAHEK
                     RLFLPLVVASSGLFFIGMAFAYFAALPLVFEFIIYFAPQGVAVMTDIGEYLGFVMTMF
                     LTFGITFEVPVFVVVLARTGIISLTKMKEVRQYVLVGAFVIGAIFTPPDVISQFLLAI
                     PLYLLYELGLFLAGMSIKKGFSASNELATKQENKPSASSNEK"
     gene            9016..12330
                     /locus_tag="PK522_05960"
     CDS             9016..12330
                     /locus_tag="PK522_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNC41312.1"
                     /translation="MIRLLSIVVFFICFIATSVLGQAIDLNDQLQSIQTKLEALNSSD
                     ETVQKKSLKEIFLKTQQVLLDHQSYLAKTQYYTEQLAQFPQQLENLEQQKTNVNSTQP
                     NFFELKQLTLSEMAQRNIETQAKLSEFQNQQQKILSELATLREQVVTNRNELATANST
                     RNIESAEIFAELDLSNEKIVEALRMYEMLQSQALADKMRMLELEALALPKAIEMATFQ
                     EEFILRPEIQQLIEESEWLAVEINLKRKAETEVAVEKSGLVLEESEWKHPILLIIVKK
                     NELLATELSHYVEQSSKLIVQKARSENRLNLIINNYAALQQRLALYEDDEALGSEIRK
                     QFKYVLTKPNTQLTQKKLNNAKLELFQLEQEKLQMQDDKTYLKRMTKGYTINPDAAVY
                     PDIMRAFQELKMFRLQVIDKFIEILREYLKELEQYASLQHQLLEKIDQYENLLKENLL
                     LTRNAKPIYQVTTNDIDKAMTWFLTDKTQSAFSKLVDRVGLVSFIVTILSFILLSVFW
                     WFLWPRYLMWEKVGKEAWGKVKQDKIIYSVRLLIFIFSQSLLFASPFFLIYLALEHSV
                     NTEINRAFSQVFYVAAIAVVFWNVLYQISRPSGLLNQHFKFPVDFVAQLHSSIRHYGP
                     VIVILCLITAFTDAIGEDMVRHVVGRVSFITVCFLLALLSLDWMGVTKVGKALYRDRH
                     FNYLKNPKLWAWIFFLQLLYLIATAAIGYYFAALYQAILIFETIAWITICGLIFLQAY
                     RGLLIAQRRITYKRAIEKREEMIALRTSIAGKAEVELVDENYMDVETISKQSHTLLTI
                     SVWGLLLLGLSSIWVDLLPTLGFLDKIVIWRTSAISEGETIVRLITLETLLVAMIILV
                     LAFIAAHNLPGTLEILVLRHLSLDTGTGYAITTLLKYVIIIIGIAIMSQKLGVEWSNI
                     HWLVAALGVGVGFGLQEIVANFVSGLILLFERPIRIGDTVTLSDVTGTVSKIHIRATT
                     LIDSDRKEVIVPNKTFITQQFINWTLSDEVTRIKIPVGIAYGSDCDNARKVLLEIAHN
                     HPLVLREPEPLAVFVDFGASTLNFELRVYAGQFSDRLDLVNDLNMEINRRFLAEGIEI
                     AFPQLDVHLHRASTKK"
     gene            12383..>12514
                     /locus_tag="PK522_05965"
     CDS             12383..>12514
                     /locus_tag="PK522_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase 2"
                     /protein_id="HNC41313.1"
                     /translation="MNKRLTIGTPLSAAATKVMLLGSGELGKEVIISLQRLGVETIAV
                     "
BASE COUNT         3637 a         2211 c         2799 g         3767 t
ORIGIN      
        1 ggggaaataa atataaggca gattgtggct tataacagct tacgcctggg atatccgtga
       61 gcatttgcca ggcaaggtcg cgctgccgct tgagtctacc ggttggcatc gtcaaatcat
      121 ggatgctttg gtatccaccc aatgcagttt ggataccaaa ttgagccgga acatttgcgc
      181 ataatcgcat cgaagccaac atatttaagc cagctatata atcttgtgcg tcgtgtttat
      241 tgcctgagac gacagcccaa ccagaacgga aacctgccgc acggtaattt tttgaaagac
      301 cattgaaggt aataaataaa acatcatcgg cgagcgatgc gactgaagta tgggtcgctt
      361 gctcatagag tatcttgtca taaatttcat cagcgtacac aattaaccgg ttctgccgag
      421 caatttcgat gatttctaat aatagttctt tgggatagag cgatcctgtt ggattattgg
      481 gattgataat cacgatggcg cgcgttctgt ctgtaatttt ggtccggatg tcgtctaaat
      541 caggaaacca atcagactgt tcatcgcaga cataatggcg agcagttcct cctgcaagcg
      601 aaacagcagc ggtccataac gggtaatcag gcgcgggtat tagaacttcg tcgttatttt
      661 ccaataaagc ttgcatggtc aacatgatga gttccgaaac accattaccc acataaatat
      721 cttccatctg cacgttagca atctgttttt cctgcgtgta atgcatgatt gctttgcgcg
      781 ccgcaaataa tccttttgag tcacaatagc cagatgctgc cgaaagatta cgaataacat
      841 cctgtaaaat ctcttcaggg gcttcaaatc caaaggaagc tggattgccg atattgagtt
      901 taatgatgtg atacccttct tcttccattt gtctcgcacg atccaaaaca ggacctcgaa
      961 tgtcataaca tacattttca agcttgctgg attttttgat cggctgcatg gtaggtcaag
     1021 tagtaataaa gaatatggta tcgtaagttg tgaatggatt ggatgagtac aaactcaatg
     1081 ctatgttacc cgacacagct atgctattgc aaaatatgta ttcgattgaa tggtggttta
     1141 agctggtatt ttgccattat ttgatgatga ttatgtttta taagtaaggg gggagggcgt
     1201 aaatgaagtt tcacctttct aatattgaag gaataaatac gttttcaggt tatggggttg
     1261 gctacgtcgt gatcaatcaa gtacgctatg atcggaattt ggttgtattt ccagatcgta
     1321 tcattgaaaa ttggtgcata agaaactctg ggcgattcgc tattgacgat tttgatgaaa
     1381 tacttaccta tcaacctgag ataatattgc tgggtacagg tgaaacgcag cgatttccta
     1441 gtcctcaatt aatcaaagcg atgcttaatg caaggattgg attggaagtg atggacacat
     1501 tttctgcttg tcgcacctac aatattttat caaccgaggg tcgcagagta gccgcagcgc
     1561 ttttagtcta acgacttttg taaaattttt gacgattaca caaataaatt agcttggata
     1621 ataaggaata ccatcaatgg gggcattagt tttttatacc tttgtttttt ttattgggta
     1681 ttttgtgatt catggcttta atttgttaat cggtcgtaag ttgttaaatc ggaaaattac
     1741 tgcactcgtc attgtttgtc tgattgcact aatgcatgga tataaaatat tgacgagtcc
     1801 actacccgct caacaggatg cagatgcttt tcaagcactt ggttactatg ttatgtttcc
     1861 aatcttagtc attgtgacgg cctgtgtgtt ttttggatta caagataagg aagatgaatg
     1921 aataccagcg tgactctgtt ttatatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnatat ctttaatttt tgataatcgt tagcgttatt tctccttttt ctttatttat
     2101 tatcttttct ttaatttgaa acatgactca cttgctcaga aatgttatac gaccagaaat
     2161 ttttgaactt tctgcgtatc atgttccacc tgctaaagga atgatcaaat tagatgcaat
     2221 ggagaatcct tatacattgc ctttaacgct tagagcggaa atcgctcaat tagcagaaaa
     2281 tgcaacgatt aatcgatatc cagatccgac agcgagcgca ttaaagagtg tcttaagaaa
     2341 aactttatca gtgcctgacg atatgtcgat ccttctgggt aacggctctg atgaaattat
     2401 tcagatgatt gcgttggctg ctgcaaaacc tggtgcagtt ctattagggg ttgatcctgg
     2461 gttcgcaatg tttcgtatca ttgctacttt ttctgggatg agttatgtag gtgttccgct
     2521 caatgaggat ttttcattaa atctcgaagc aatgcgtaca gcgattactc gctatcaacc
     2581 cgccattatt tttcttgcct atcccaataa tccaacaggt aacctatttg atatacaagt
     2641 gatacatcag attattcagg aagcacctgg tattgttatt atcgatgaag cctatcatgc
     2701 ttttgctggc gttagtttta tggatcagtt ggcgcaattt cctaatttac tattgatgcg
     2761 aaccttatca aaattgggtc ttgcagggtt aagattaggg ttgctgattg gaaggtctga
     2821 atggcttgag caatttgaga aactgcgttt accctataat attggcgtca tgacccaatt
     2881 aattgctgaa aaggtattgc agcattatga agatttatta aagcaaacgg cagccatcaa
     2941 aacagagaga agtaagctat tatcagaact tagtggttta agcggaataa cgacttttcc
     3001 ttcagaagct aattttattt tatttcgtat taagagagcg agtgaagtgt atgcgttact
     3061 caagcagaag ggtgttctca ttaaaaatct ggatggagct catcccttat taaaggattg
     3121 tttacgtgtt acggtgggca cacctcatga aaatgatcaa ttctatcacg cgcttttaca
     3181 agtacttgaa gacacaaatt gttagattga tatgtatcgt aaggcttaac tgataatatt
     3241 tttatgcgcc agatcaaagt tacccggaat acgttggaaa cgcagataag cgttgaggtt
     3301 aatttggacg gaagtggtca ttcaaagttg aatactggta taccatttct agatcatatg
     3361 ttagatcaga ttgctcgtca tgggatgatt gacatcacag tggaagcaaa aggagatttg
     3421 catatcgatg cacaccacac ggttgaagat attggaatta cacttggtca agcattagca
     3481 aaagcttggg gtgataaaaa aggggtgcgt cgttatggtc atgcctatgt tccactcgat
     3541 gaagcgcttt ctagagtcgt gctggattta tcaggtcgac cagggcttgg ttatcgggtc
     3601 gaatttgttc gtgctcgcat tggtgagttt gatgtcgatt taattcacga attttttcaa
     3661 ggattggtta atcatgctat gattacactc catattgata atctttttgg agataatgcg
     3721 caccatcaag ctgaaaccgt ttttaaggct tttggaagag cattacgtat ggcaattgag
     3781 actgattccc gcgcaattaa tctaatccca tccacaaaag gttcattgta agcgctactc
     3841 gtaaccgcat taattattaa gtatgacagc tattgcgatt gttgattacg gaatgggtaa
     3901 tttacgttca gtatccaagg cccttgaacg agttgctccc tacgcgacag ttcaagttac
     3961 gagtgaccca aagattgtag ataaagcttc gcatatcgtt gttcccggcc aaggcgcaat
     4021 gccgcattgt atgcaagagt tagataaatt tggtctaaaa gacgttgtaa ttgatgctgc
     4081 caggaacaag ccatttctag gtatttgtat tggtttgcaa atgctgtttg atgaaagtga
     4141 agaaggtaat gtgagaggct tgggtgtctt cccagggaaa gttgtgcact tctcggctgg
     4201 tggcgaagct gatgttcagg gcggtaagtt aaaaataccc catatgggct ggaatcaagt
     4261 ttatcaaaca atgaatcacc cgctatggga tggaattaga agcgatgctc gcttttactt
     4321 tgttcatagt tattatgtga agtcagagga tccaaactta tcagcagcct actcgcttta
     4381 tcctacccca tttacatgcg cgatcgctaa agataatatt tttgcagtac aatttcatcc
     4441 agagaaaagt cacattgctg ggttaacatt attaagtaat tttgctaaat gggaacctca
     4501 ttccagtcat tagtacaatt caattcatcg tttcattccg tttttttatt atcaattatt
     4561 tatcctaaca acatatgttg attattcctg cgatagacat gaaagatggt caatgcgtgc
     4621 gactaaaaca gggtgatatg gaagccgcaa ctgttttttc tgaaaaccct gatgaaatgg
     4681 cactacaatg gttgaaccaa ggtgcgcgtc gcttacattt ggtggatttg aatggagctt
     4741 ttgctggtaa accgaaaaat gagtctgtta ttcgctcaat cgttaaagcg cttaatggca
     4801 atatcccaat tcaattaggt ggaggaattc gtgacttaga aacgattgaa cgctatctgg
     4861 atcagggaat tacgtatgta atcattggga ctgccgcagt aaaaattcca ggttttttgc
     4921 atgatgcttg ttatgcattt cctggtcaaa ttatggttgg ccttgatgcg aagaatggta
     4981 aagtagctgt tgatggctgg tcgaaagtga cggggcatga tgttgtcgat ttagccaaga
     5041 aatttgaaga ctatggcgta gaagcgatta tttataccga tattgggcga gatggaatgc
     5101 ttagcggggt taatattgaa gcaaccgttg cgctagctcg ggaattaacc gttccggtag
     5161 tagcgagtgg aggcatttct aatttagaag atatcaaagc gctttgcaaa atcgaagcag
     5221 aaggtgtaat gggtgcaatc accggtagag ccttatatga gggtacgctg gatttcaaac
     5281 aggctcaaga cttggctgat aaattaagtg taacagccta aattaagtgt gcatcctgtg
     5341 atctgatcat tactttacta ttcacttttt tctctctaat atttttcttt actaaggttt
     5401 ttaggcaatg ggtcttgcca agcggattat tccctgtctt gatgttaaca atggacgtgt
     5461 tgttaaaggg gtcaattttg tatcgttaaa agatgcgggt gatcccgttg agatcgcacg
     5521 gcggtatgat gaacagggag ctgatgagct tgtgttttta gatataacgg caagttcgga
     5581 taatcgggac ttgatcctgc atatcgtgga agatgttgcg tcacaagtat ttataccgtt
     5641 gacggtaggg ggtggcgtac gtaaagtaga ggatgtgcga agattactga atgccggcgc
     5701 tgataaagtc agtattaaca cttcggcagt actaaatcca caactcgtag cagatgcggc
     5761 aggctattat ggtgctcagt gtatcgtggt tgccatagat gcaaaacaag ttggtgagca
     5821 aggtagccag gttccgcgtt gggaagtatt tacccatggc ggccgaaatg ctactgggct
     5881 tgatgcgatt gagtgggcta aaaagatgca agcgttgggt gcgggtgaaa tattgctgac
     5941 aagcatggat cgagatggta cccgacatgg attcgatctt gctttaacac gaacgctttc
     6001 cgatgcaatc gatttgcctg taattgcaag tggcggggta ggaagcttga atcacctggt
     6061 agatggaatt cttcaagggc atgctgacgc ggttcttgca gcaagtatct ttcattatgg
     6121 ccagcatacg atacttgagg caaaagaatt tttatcccag catggtatcg aagtacgact
     6181 ctaacaataa ctaggttgtc atcatttttt aattttctgg aatcagaatg ccagcttctt
     6241 gggttgataa aattaattgg accattgatg gattagtgcc ggtcattgca caggaagaag
     6301 caaccgggac tattttgatg tttgcttgga tgaataaaga agcggttaaa ttgacggttg
     6361 aaacgaaggt ggtagtttat tggtctcgtt ctaagcaaag gctctggaga aaaggtgaag
     6421 aatctggcca tacacaaaag gtcaaagcaa tttatctgga ttgtgatgat gatgttctac
     6481 tgattcaagt agaacaaatg ggcgggatag cttgtcatac cggtagaaaa agttgttttt
     6541 ttaataaact ggaaggggat caatgggtag ttaatgcccc cataattaaa gatccttcag
     6601 aaatctatca ataaatgtca cacgcctcca tacttcatca tctcgcaacc gtcattgatg
     6661 acagaagaac agccgaccca tcaacctcat atgttgctaa gctattgaat agtggtcaag
     6721 ataaaatctt aaaaaaagtt gctgaagagg cggctgagac gatcatggca tccaaagatg
     6781 gcgatacgac acatgtggtt tatgaagttg ctgatctctg gtttcattgc cttatcctac
     6841 ttgcacacca tggcctgaga ccagaagatg tcctagcgga attagcacga agagaaggat
     6901 tatctgggat agaagaaaaa cggctacgca atacggaaaa gtaaatgagc gattgtattt
     6961 tttgtaaaat tgtaagaaaa gaaattcctg caaagattat ctatgaagat cagcactgcc
     7021 ttgcttttca tgatataaac cctgctgctc ccgttcattt cttaattatc cctaaacttc
     7081 atattgcttc gctatatgaa gttgatgata cccatcagct cattcttgga aaaatgttag
     7141 gcctggtgcc taagttggcc aaacaggaag ggtgtgaaca tggttttcgt actatcatca
     7201 atacaggaaa ggtaggcggg caagaagttt ttcatcttca tattcatgtg ataggcagta
     7261 aaaatgtttt gccggctatg attcaccgta gtcaatagtg attctattga gaggaaataa
     7321 aaatgggaac atttagtatt tggcattggc tggttgtact tgcaatcgta gtgctagttt
     7381 tcggaaccaa gaaattgcgt aatttaggga gtgatcttgg caatgcagtg aaaggtttta
     7441 aagaaggcat gaaaggaagc gaaggagaac caatggaatc tcctgcaaat actcagcagc
     7501 aaattgcgaa tcgatctacc gaagacgtta gtataaaaga aaagaacgag actaaattat
     7561 aaagcgttta gttatattcc aataagggag ttgaattaag ctctggaaat tcaatatcct
     7621 gctataagaa cttaaactag agtagtttga attctaaatt tcccggaagg attttaggct
     7681 tatgtttgat attagcttcg ctgagttgct cgtcatttca gttgtagcat tagttgtcat
     7741 cggcccggag agacttccac gagtagctcg aaccttagga ttcttgttgg gtcgcgcacg
     7801 tcgctatgtc gaaacggtca aaaatgatat acggaatgaa atagaattgg acgagttaag
     7861 gcggattaaa gacacggttc aggatacagc gcaatccatt gaaaaatccg taaaacaaga
     7921 gattcagcaa attcaagcaa gtgtccagcc cactgcatcc gatagccacg ataaaaaaat
     7981 caaagatacc tctcccgtaa ataacaatca cgcagggcat gaggccacgc ccattgcgtc
     8041 tcaatcgatt tcgaactcaa agggcgactg attcgactaa taataattag gactgtcgtg
     8101 ttgagagata acagtatgct ttcatagagt tttttctaga gtgagtcata aaatatggac
     8161 gaggaaggta gttttatttc acatttggtc gaattacgca ctcggttaat ccggattatt
     8221 acggtattag tggttggttt tataccctgt gcttattttg cccgtgagct atacacattg
     8281 ttagctcagc cccttctcga aaaactcccg caaggtgggc aaatgattgc aaccgcagtg
     8341 gcgacacctt ttttcgtgcc aatgaaagta gcgctgatga cagcattttt attaacgctt
     8401 cctcatacgc tgtatcagat ttgggcattt gtcgcccccg ggctatatgc ccatgaaaaa
     8461 cgattattct taccgttggt cgtggcgagt agcggtttat tttttatagg gatggccttc
     8521 gcctattttg cagctttacc cttagttttt gagtttatta tttattttgc gccgcaaggg
     8581 gttgctgtca tgacagatat cggggagtac cttggttttg tcatgacgat gtttttgact
     8641 tttgggatta cgtttgaagt accggtattt gtagttgtac tggctcgaac cggaattatt
     8701 tcgttaacca aaatgaaaga agtaaggcag tatgtgcttg tcggtgcttt tgtgatcgga
     8761 gcgattttta ccccaccaga tgtcatctcg cagttcttat tagcgattcc actttattta
     8821 ctctatgaat taggattatt ccttgcagga atgtcaataa agaaaggttt ctcagcctcg
     8881 aatgagttag ccacaaaaca agaaaataaa ccaagcgcta gtagcaatga aaaataagcg
     8941 atcgaaacgg attatttacc aacatctggt tgcctttatt gagctgtatt ttaagatatt
     9001 caggccatat cctaaatgat tcggttattg agtattgttg ttttttttat ttgttttatt
     9061 gctacctcgg tattgggtca agcgattgat ttaaatgatc aacttcaatc gatccaaact
     9121 aagttagaag cgctcaatag tagcgatgaa acagtccaaa agaaaagtct caaagagatt
     9181 tttcttaaaa cacaacaagt tttacttgat catcaaagct accttgccaa aactcaatac
     9241 tatactgagc aattagcgca attcccacag caactagaaa atctagagca acaaaaaact
     9301 aatgtaaatt cgactcaacc taatttcttt gaattgaagc aactcacatt aagtgaaatg
     9361 gcgcagagaa acattgagac gcaggccaaa ttatcagaat ttcagaatca acagcaaaaa
     9421 atcctatctg agctagccac attgcgagag caagttgtca ctaatcgtaa cgagctagcg
     9481 actgcaaaca gtacgcgtaa cattgaatcg gctgaaatat ttgctgagct tgatttaagt
     9541 aatgagaaga ttgtagaagc attacgcatg tatgaaatgc ttcaaagtca ggcattggcg
     9601 gataaaatga gaatgctcga gctagaggca ctcgcgttac ccaaagccat cgaaatggcg
     9661 acattccaag aagaattcat acttaggcca gagatacaac aattaattga agaatcagaa
     9721 tggttagcgg ttgaaattaa tttaaagcga aaagccgaga cagaggttgc tgtcgagaaa
     9781 tcagggctgg tcctagaaga aagtgagtgg aaacatccca ttcttctcat catcgttaag
     9841 aaaaatgaat tgcttgcaac agagctgagt cactatgtag aacaatcttc taagttaatc
     9901 gtacagaaag cacgtagcga aaatcgattg aatctaatca tcaataatta tgctgcgttg
     9961 cagcagaggc ttgcattgta tgaggatgac gaagcgctag gttcagaaat taggaaacaa
    10021 tttaagtatg ttttaacaaa acctaatact cagctcacac aaaagaaact taacaatgca
    10081 aagctggagc tgtttcaact ggagcaagaa aagcttcaga tgcaggatga taaaacttat
    10141 ttaaaaagaa tgactaaggg ctatacaatc aacccggatg ctgcagttta ccccgatatt
    10201 atgcgggcat ttcaagaact taaaatgttc cgcttgcaag ttattgataa attcatagaa
    10261 attttgcgtg aatatttaaa agagttggag caatacgcga gcttgcagca tcaattattg
    10321 gaaaaaattg atcaatatga aaacctactt aaagaaaatc tattactaac ccgtaatgca
    10381 aaaccaattt accaggtaac taccaatgat attgataaag caatgacatg gtttcttacc
    10441 gataaaaccc agagcgcttt ttcaaaacta gttgatcgcg tagggctagt atcattcatt
    10501 gttacgatcc tttcatttat tttgctcagc gtattctggt ggtttctgtg gccacgctat
    10561 ttaatgtggg agaaggtggg aaaggaagca tggggaaaag ttaagcaaga taagataatt
    10621 tactcggtac gactgctgat tttcatattt tctcaatcat tgttgtttgc gtcaccattc
    10681 ttcctcattt atctcgcgct cgaacatagt gttaatactg agatcaatcg tgcattttcc
    10741 caggtttttt atgtagcagc gattgcagtt gttttctgga atgtgttgta ccagattagt
    10801 cgaccatccg ggctacttaa ccaacatttt aaatttccag tagattttgt agcccagtta
    10861 cattcgagta tcagacatta tgggccggtt attgttattc tctgcttgat tacggctttc
    10921 acggatgcaa ttggtgaaga catggtgcgc cacgttgtgg gaagagtgag tttcatcaca
    10981 gtgtgcttct tgcttgcttt attgtcttta gattggatgg gggtaaccaa agtcggaaaa
    11041 gcattgtatc gagaccggca ctttaattac ctaaaaaatc caaagctatg ggcttggata
    11101 ttctttttgc agctcctgta tttaattgct actgcggcga taggatacta ttttgctgct
    11161 ttgtaccaag ccatccttat ttttgagacg attgcttgga ttacgatttg cggtttgatt
    11221 tttctgcaag cttatcgtgg cttattaata gcacagcgta ggattaccta taaaagagca
    11281 attgaaaaaa gagaagaaat gatcgcgtta cgtacttcga ttgccggtaa agcggaagtg
    11341 gaattggttg atgagaatta catggatgtc gagacgatca gtaagcaatc tcatacatta
    11401 ctaacaatca gtgtatgggg tcttttgtta ctcggtttga gcagtatatg ggttgatttg
    11461 ctaccgacac tgggattctt agataagatc gttatttggc ggacttctgc gataagtgaa
    11521 ggagaaacca tcgtccggtt aatcacactt gaaacactgc tggtggcaat gattattctc
    11581 gtgctcgcat ttatcgcggc acataattta ccgggtactt tggaaatttt ggttttgcgc
    11641 catttatcac tggataccgg tacaggctat gccataacga ccttattaaa atacgtaatc
    11701 attatcattg gaatagcgat catgtcacaa aagctcggtg tggaatggtc caatattcat
    11761 tggttagtag ccgcgctggg tgtcggagtg ggatttggtt tgcaagagat tgtggctaat
    11821 ttcgtatcag gcctaatttt actatttgaa cgtccgatcc gaatcggaga tacggtgaca
    11881 ttaagcgatg taactggtac ggttagtaaa attcatattc gtgccacgac gctgatagac
    11941 tcagatcgta aagaagtcat cgttcccaat aagactttta ttactcagca atttattaat
    12001 tggacactca gtgatgaggt aacgcgcatt aaaataccag tcgggattgc ttatggatca
    12061 gattgtgata acgcaagaaa ggtattgcta gagattgctc acaaccaccc attggtattg
    12121 agagagcctg agcctctggc tgtttttgtt gattttggcg caagcacctt gaattttgag
    12181 ttacgagtat atgcgggtca attttcggat cgccttgatc ttgttaatga cttaaatatg
    12241 gagatcaatc gacgattttt agctgaaggg atcgagattg cttttccaca actggatgtc
    12301 catttgcatc gtgcatcaac gaaaaaataa tcagaagatg agtatgatga taagaagggt
    12361 attattttaa gggttttatg gtatgaataa acgattgacg attggcacac cactcagtgc
    12421 agcagcaacg aaagtaatgt tattgggtag cggtgagctt gggaaggaag tcattatttc
    12481 attacaacgg ttgggcgttg aaaccattgc tgtc
//
LOCUS       DAOQLJ010000063        12460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1941_length_12460_cov_6.575252, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000063 DAOQLJ010000000
VERSION     DAOQLJ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12460
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1941_length_12460_cov_6.575252"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PK522_05970"
     CDS             complement(<1..458)
                     /locus_tag="PK522_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNC41314.1"
                     /translation="MTIREQAIAGIKWTAGGKLAAQAITWGITLIVMRLLHPEDYGLL
                     AIASVFLAFMMMLSEAGLAPALVQKQELSKIALQQVFAIIIVINLALLALLNLLAPII
                     ANFFNDERLILILNVMSLQFLTVIAITIPQVLLYRELKFKYLSIIDFIAV"
     gene            complement(695..2083)
                     /gene="cysG"
                     /locus_tag="PK522_05975"
     CDS             complement(695..2083)
                     /gene="cysG"
                     /locus_tag="PK522_05975"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041357033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HNC41315.1"
                     /translation="MDFFPIFLNIRDRKCLVVGGGEIAARKVELLLKANALVTVISPT
                     LGETLEAFSSQSQISYIRDIFHPKYLTNYTLVISATNNHQVNQQVWEEAIKANIPVNV
                     VDNLSLCSFIMPAIIDRSPVVIAISSSGKSPVLVRMLRTRLETLIPGAYGKLAQYLGE
                     FRDLVKQHFILPEQRRFFWEKLLQSPFVEMVLSGKDQAAQQYLIKSIENDPKDLPQGE
                     VYLVGAGPGNPDLLTLRALQLMQQADVIVYDRLVSKSIMDLTRRDATRIYAGKERNNH
                     TLSQESINELLVRLAKDGKRVLRLKGGDPFIFGRGGEEIETLSEHRIPFQVVPGITAA
                     SGVASYAGIPLTHRDYAQSCTFVAGHLKHNSLDLDWNTLARPQQTIVVYMGLLGLPIL
                     CEQLIAHGLPATTPAAIVQQATTNNQRVVIGSLDTLPSLAMNAHIVPPTLIIIGEVVK
                     LHQKLTWYHYDY"
     gene            complement(2208..3203)
                     /locus_tag="PK522_05980"
     CDS             complement(2208..3203)
                     /locus_tag="PK522_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC41316.1"
                     /translation="MLNKRSFISVIGGGLLLILSSQLSARAIVMIDGSSTVYPITEAV
                     AEDFQAMKKGEIRVTVGISGTGGGFKKFCRAEIDIANASRPIQKAEMETCKAAGVEYI
                     ELPVAFDALTIAINPKNTWSKTITVAELKAMWEPSAQGKITKWNQINPAWPDEKIKLY
                     GAGADSGTFDYFTEAIVGKAKSSRGDFTASEDDNVLVQGIASDKFSLGFFGFAYYIEN
                     QKKVSAAAVDNGKGGVTPSAEAVENGSYQPLSRPIFIYVNAKALDKPEVREFINFYMN
                     NAPALVKEVKFFPLPAEVYQLNLDHLEKRKIGTVFGGESKIDLRIEELLKLEKQM"
     gene            complement(3370..4749)
                     /gene="glmM"
                     /locus_tag="PK522_05985"
     CDS             complement(3370..4749)
                     /gene="glmM"
                     /locus_tag="PK522_05985"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNC41317.1"
                     /translation="MKKKYFGTDGIRGRVGEFPITPEIILHLGYSAGKVLLESDWHLK
                     KGEKPVVLIGKDTRVSGYLLESALEAGFSAAGVDVLLSGPLPTPAIAYLIRALRLQAG
                     IVISASHNPFDDNGVKFFSSEGTKLPDEMELKIEMGLEAPIRTMPSVKLGKVQRLKDA
                     TGRYIEFCKSAFPYHLDLRGLRIVIDCANGATYHIAGHVLHELGADVVTICANPDGFN
                     INHQCGATHCATLQSAVKEHAADLGIALDGDGDRVIMVDNDGVIYDGDLLIYIIAKHR
                     YSKGMLRGGVVGTSMTNLAIEKAMMLLNIPFERSNVGDRYVLELLNKNGWQLGGESSG
                     HIVCLDKHTTGDGIISALQVLYALRDSKLNLREATGDIKLFPQRLINVRVAKGLNYLS
                     NPSIQAIVQQAENDLNSEGRVLLRASGTEPLIRVMVEGSNQQKVNSWAEKIANSVQEI
                     AQQQESKQS"
     gene            complement(4808..5578)
                     /gene="folP"
                     /locus_tag="PK522_05990"
     CDS             complement(4808..5578)
                     /gene="folP"
                     /locus_tag="PK522_05990"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNC41318.1"
                     /translation="MGIINVTPDSFSDQGRYAASEKAIEHAIKLIADGADLLDIGGES
                     SRPGSACVSLDEELNRVIPVIEALKSAAIPISVDTTKPEVMQAAVQAGVSMINDINAL
                     RTEGALKIIAESGVMVCLMHMQGLPKTMQDNPLYADVVSEVREFLQQRIQIAQDWGIS
                     NDQLVIDPGFGFGKTLQHNLELFRSLDQFTTLNIPVMVGVSRKSMLGTITGNDVNHRT
                     YASIAAAVLAAEKGAKILRVHDVKATKEAILVFNALNR"
     gene            complement(5761..7662)
                     /gene="ftsH"
                     /locus_tag="PK522_05995"
     CDS             complement(5761..7662)
                     /gene="ftsH"
                     /locus_tag="PK522_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNC41319.1"
                     /translation="MNNLIKNMAIWLVIAIVLMTVFNQFTVRQSAQSPIEYSQFINEM
                     NQGRIAKVTIDGRTLKGTKSDGRQFITFAPSDPWLVSDLLRAGVIVEAKPEEEPSILM
                     SIFVSWFPMLLLIAVWIFFMRQMQGGGRSGGAFSFGKSKARMLDKNSNHVTFADVAGC
                     EEAKEEVAELVEFLRDPTKFQKLGGRIPRGVLMVGNPGTGKTLLARAIAGEAKVPFFS
                     ISGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRQRGAGLGGGNDEREQ
                     TLNQLLVEMDGFEGNMGVIVIAATNRPDVLDPALLRPGRFDRQVTVPLPDIRGREQIL
                     QVHMRKVPISPDVHADVLARGTPGMSGADLANLVNEAALFAARSNKRLVDMDDFERAK
                     DKILMGAERRSVVMPENERRNTAYHESGHAVVAYLLPKTDPVHKVSIIPRGRALGVTM
                     QLPTEDRFSMDRDQILQTISVMFGGRIAEEIFMNQMTTGASNDFERATETTRRMVTQW
                     GMSDVLGPMVYGENEGEVFLGRSVTTHKNVSEATMQKVDAEIRRIIDEQYALARKLIE
                     ENRDKIEAMTKALLEWETIDSEQIKDIMENRPPRPPQPPQSKSSAAPKEDNKTVSSTA
                     ANEGSAQEV"
     gene            complement(7807..8430)
                     /locus_tag="PK522_06000"
     CDS             complement(7807..8430)
                     /locus_tag="PK522_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HNC41320.1"
                     /translation="MKSNKVNRAWIHEHVNDFYVKQAQKAGYRSRAAYKLIEIAERDQ
                     LFSPGMIVVDLGAAPGSWSQVCRERIGKSGKIIALDLLNMLPLDGVAFIQGDFSEEKV
                     CEMIENQLAGDLADLILSDMSPNLAGIGMSDQARSINLAELALAFANNKLKPGGSFLV
                     KIFQGSYVDQYIRDMRFHFNKVMIRKPKASRSKSKELYLLGLGKIIC"
     gene            complement(8636..9112)
                     /gene="greA"
                     /locus_tag="PK522_06005"
     CDS             complement(8636..9112)
                     /gene="greA"
                     /locus_tag="PK522_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNC41321.1"
                     /translation="MSPIPLTVAGAEKLRAELHEMKTVQRPAVIAAIAEARSHGDLSE
                     NAEYDAAKERQGFIEGRIAELESKLANAQIINPALIDADGACVFGATVELKDFGGNVT
                     VIYQIVGDDEANLKEKKISISSPIARALIGKYAGDIVEVVAPAGVREYEILNIQYI"
     gene            complement(9149..12358)
                     /gene="carB"
                     /locus_tag="PK522_06010"
     CDS             complement(9149..12358)
                     /gene="carB"
                     /locus_tag="PK522_06010"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNC41322.1"
                     /translation="MPKRTDIKSILVIGAGPIIIGQACEFDYSGVQACTALREEGYRI
                     VLVNSNPATIMTDPEMADATYIEPITWPMLEKIIDIERPDALLPTMGGQTALNCALDL
                     VKYGVLDKYGVELIGASREAIDKAEDREKFKQAMAHIGLSSARSAIAHSMEEALQVQA
                     MIGYPVIIRPSFTLGGTGGGIAYNRQEFIEICERGLDASPTRELLIEESVIGWKEFEM
                     EVIRDKQDNCIIICSIENLDPMGIHTGDSITVAPAQTLTDKEYQIMRNASIAVLREIG
                     VETGGSNVQFAINPADGRLLVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLNE
                     LSNDITGGVTPASFEPTIDYVVTKIPRFAFEKFPQANDRLTTQMKSVGEVMAIGRTFQ
                     ESFQKALRGLEIGVDGLDEKSSDLDLIAAELGHPGPERIWYVADAFRHGMAFDEIYQL
                     THIDPWFLHQIENLIQQEQSLTDSTVDSIDTDLLRQLKRSGFSDRRLAKLLNSSELAI
                     RTRRLNEKIYPVYKRVDTCAAEFSTDTAYLYSTYEEECEANPSTRKKIMVLGGGPNRI
                     GQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDISDRLYFEPLTLEDVLNIV
                     AVEKPNGVIVQYGGQTPLKLARDLERFGVPIIGTSPDMIDCAEDRERFQDLLNRYNLK
                     QPMNRVARNVEMALAAATEIGYPLVVRPSYVLGGRAMEIVHEQKDLERYMQEAVKVTN
                     DSPVLLDHYLNNAIEVDVDAIFDGKTILIGGIMEHIEEAGVHSGDSACSIPPFSLSTE
                     LQQELRRQTEVMARALNVIGLMNVQFAIQNDIVYVLEVNPRASRTVPFVSKATGLQLA
                     KISARCMVKKSLYDQAIFKDIEPTFFSVKEAVFPFSKFSGVDTILGPEMKSTGEVMGV
                     GNTFAEAFVKSQLATGIKLPISGKIFISVRASDKLEAVTLAKKFIELGFTIYATQGTA
                     EVMIASGLNVHKVNKVAEGRPHIVDMIKNGEICLIINTVANKRRAIQDSYSIRHAALL
                     AKITYYTTMAGARAAYMGISNMRELHAYSMQMLHHQTIQ"
BASE COUNT         3762 a         2730 c         2173 g         3795 t
ORIGIN      
        1 ataacagcga tgaaatcaat aatcgataag tatttaaatt taagctcgcg ataaagtaat
       61 acttgtggaa tcgttatggc gattacagtt agaaactgta aagacatcac gtttagaatt
      121 aaaataaggc gttcatcatt gaaaaaatta gcgataattg gtgccaataa atttagtaat
      181 gctaataatg ctaaattgat aacgataatg atcgcaaaaa cctgttgtaa tgctattttg
      241 ctcaattcct gcttttgcac aagcgctggc gcaagtccag cttcagagag catcatcata
      301 aatgctagga aaacagatgc tatagctaat agtccataat cctcgggatg taacaatcgc
      361 ataacaatga gtgtgatacc ccaagtaatt gcttgagcag cgagttttcc tcctgctgtc
      421 catttaatcc ctgcaattgc ctgttcacga attgtcatta caattaaaat atgaagaaaa
      481 taaaacgaaa acagcgcttg gtacactgtt cagcgtgtga atacaaccta tttgaataat
      541 atcttaagct ttgagtgaat ggacttatga gttgagtaaa ttatcagttg catattatca
      601 cttagtgaca aattaatatt gtgcgcaatg ttaagtgtaa gaaatttaag aatattgtat
      661 aaaagaatac tcctcaataa agttttagta ggattcaata atcatagtga taccaagtta
      721 gcttctgatg aagcttaacg acttcaccaa taataataag tgtcggaggc acaatatgtg
      781 cattcatcgc aagggaagga agtgtatcta aagacccgat tacaacgcgt tgattattgg
      841 tcgtagcttg ctgcacgata gctgcaggcg ttgtagcagg taggccatgg gcaatgagct
      901 gttcacataa gataggcaat cccagtaaac ccatatatac cactatcgtt tgctgaggtc
      961 gagctaaagt attccaatca agatctaagc tattatgctt aagatgtcct gcaacaaaag
     1021 tacaggattg agcataatca cgatgggtca gcggtatacc tgcatatgag gccacccctg
     1081 aagctgcagt aattccaggt actacctgaa acgggatacg atgctcagat aaggtttcta
     1141 tttcttcccc gccccgacca aaaatgaaag gatcaccgcc ttttaaccgt agcacacgtt
     1201 ttccgtcttt ggcgagacgc actaataact cattaatcga ttcttgcgat aacgtatgat
     1261 tattacgctc tttacccgca taaattcggg ttgcatcacg tcgagtgaga tccattatac
     1321 ttttcgatac aagacgatca taaacaatga catctgcttg ctgcattaat tgcaaagccc
     1381 ttaatgttaa taaatccgga tttccagggc ctgcgccaac taagtaaact tcaccctgcg
     1441 gtagatcttt tgggtcattc tcaattgatt ttatgaggta ttgctgagcg gcctggtctt
     1501 tccctgaaag aaccatttcg acaaatggac tttgcaaaag cttttcccag aaaaatctgc
     1561 gttgctctgg aaggatgaaa tgttgtttta ccaaatcacg aaattccccc agatattgtg
     1621 ccaacttacc gtaagcacct ggtattaggg tttccaaacg ggtgcgcagc attcgaacta
     1681 acacaggtga tttaccacta ctggaaattg caattaccac tggagagcga tcaataattg
     1741 ctggcatgat gaaagaacac aagctcagat tatcgacaac attaacgggg atatttgctt
     1801 tgatggcttc ttcccagact tgctgattta cttgatgatt attagtagca gaaattacta
     1861 aggtgtaatt cgtgagatat ttagggtgaa aaatatctcg aatgtaactg atttgagatt
     1921 gagacgaaaa cgcttctaat gtttcgccta atgtgggaga aataactgtg acaagagcat
     1981 ttgccttaag caatagctct acttttctag cagctatttc tccgccccca actaccaaac
     2041 attttcgatc tcggatatta agaaagatag gaaaaaaatc catatgatca ccgagcaaga
     2101 tgatttggca agttaagtct ttcgctgatt aaaattctct agagcctaaa gcttatcagt
     2161 aatcaattta aaaagttatt gttcaaaatc gttcagaaat gtcaaattta catctgcttt
     2221 tctaatttaa gtaattcttc gattctgaga tcaattttac tttctccacc gaatacggtg
     2281 ccaatttttc ttttttccaa atgatctaag ttaagctgat aaacctcagc tggtaatgga
     2341 aaaaatttta cttcctttac caaagcaggc gcattattca tatagaaatt gataaattct
     2401 ctgacttccg gtttatctaa tgcttttgcg ttgacataaa tgaaaatagg tcgagataac
     2461 ggttgataac tgccattctc aacagcttca gctgaaggag ttaccccccc tttgccatta
     2521 tcaacagcag cagcacttac ctttttttga ttctcaatat agtaagcaaa tccaaaaaaa
     2581 cctaaactaa acttgtcact agcgatgcct tgaacaagta cgttatcatc ttcagaagca
     2641 gtaaaatcac ctcgactcga tttggctttg cctacgattg cttctgtaaa gtaatcaaat
     2701 gtcccagaat cagcgcctgc gccatataat ttaatttttt catctggcca agctggatta
     2761 atttgattcc atttagtgat ttttccttgc gcagaaggct cccacatcgc ttttaactca
     2821 gcaactgtaa tggttttact ccatgtgttc ttaggattta tggcgattgt caatgcatcg
     2881 aacgcaacgg gtaattcaat atattcgaca ccagccgctt tgcaggtttc catttctgct
     2941 ttttgaatag gtcttgacgc attagcaata tcaatttcag cacggcaaaa ctttttaaat
     3001 cctccacctg tcccagatat tccaacagtt actctgattt cacctttttt cattgcttga
     3061 aaatcttcgg caactgcttc agtaatggga tataccgtac tagacccatc aatcatcaca
     3121 atcgctctcg cgctaagctg gctacttaaa ataagtaata gccccccacc aataacgcta
     3181 ataaaacttc ttttattcag catgttcaaa ataaatctct ccatattaaa gcaagttgat
     3241 caaattgatc agaatcacca tttacctttt ccttaaactt taagaaaaga taatattaat
     3301 taataattat tacagaattg ttacatgtaa taatttttga ttcgctttca atttaataat
     3361 gaccatcaat tacgattgtt ttgattcttg ttgttgagca atttcttgta cagaattagc
     3421 gattttctca gcccaactat taactttctg ctgattactg ccttctacca tgactcgaat
     3481 taatggctca gttcctgatg cccgaagtaa cactcggccc tcactgttta gatcattttc
     3541 agcttgttga acgatggcct gaatagacgg attgctgaga taattcaatc cttttgctac
     3601 ccgaacatta ataaggcgtt gtggaaatag tttaatatca ccagtggctt ctcttaagtt
     3661 caattttgaa tcacggagtg cgtacagaac ttggagtgca gaaataatac catcgccggt
     3721 ggtatgcttg tctaaacaaa cgatatggcc ggagctttcc ccaccgagtt gccatccgtt
     3781 tttattgagc agttcaagaa catatcgatc accaacatta gatctttcaa aaggaatatt
     3841 caatagcatc atcgcttttt ctatagcgag attagtcatt gaagttccta ctactccccc
     3901 tcttagcatg cctttactgt agcgatgctt agcaatgata taaattaaaa gatcaccatc
     3961 atagataaca ccatcattat caaccatgat tactcgatcg ccatctccat ctaaggctat
     4021 acccaaatcg gctgcatgtt ctttgacggc agattgcaaa gtagcacaat gagtagcgcc
     4081 gcattggtga tttatattga atccgtcagg gtttgcacag atagtcacta catctgctcc
     4141 tagttcatgg agtacatgcc ctgcgatatg ataagttgct ccatttgcac aatcgatgac
     4201 aatcctcaat cctctcaaat ctaaatgata agggaaagcg cttttacaga actcaatata
     4261 tcttccagtc gcatctttta agcgttgcac tttaccaagc tttactgaag gcatcgtccg
     4321 aatcggtgct tcaagtccca tctcaatttt taattccatt tcatccggta attttgtacc
     4381 ttcagatgag aaaaatttga caccattatc atcaaatgga ttatgggaag cggaaattac
     4441 aatccctgct tgtagccgta atgctcttat taaataggca atcgctggtg ttggtaaagg
     4501 gcctgataag agtacatcca cacctgctgc agaaaatcca gcttctaaag ctgattctaa
     4561 taaataacca gatacacgcg tatcttttcc aattaataca acaggctttt ctcccttttt
     4621 taggtgccaa tccgattcta acaatacctt accagctgaa taacctaagt ggagaataat
     4681 ttcaggtgta attggaaatt cgccaacacg tcctcgtatt ccatctgttc caaaatattt
     4741 tttcttcatt agtcagatat tctgaatgag aaattagtaa tttattaatg gactatctta
     4801 ttttcaatca tcgatttaat gcattaaaaa ctaaaattgc ttcttttgtt gctttgacat
     4861 catgtacgcg caaaatttta gctccctttt ctgcagctaa tacggctgca gcaatactcg
     4921 cataagtgcg gtgattgacg tcatttccag taattgttcc tagcatcgat ttacgtgata
     4981 ctcctaccat aaccgggata ttaagagtag taaattgatc aagtgatcga aataattcta
     5041 aattatgttg caaagtttta ccaaagccaa aaccaggatc gattactagt tgatcatttg
     5101 agatgcccca atcttgagcg atttgaattc gctgctgcaa aaactcccta acctcagaaa
     5161 ccacatcagc gtaaagtgga ttatcttgca tcgttttagg cagtccttgc atatgcatca
     5221 agcataccat aactccactc tcagcgataa tttttaatgc accttccgtt ctaagtgcgt
     5281 taatatcatt aatcattgaa acacctgctt gaacagcagc ttgcattact tctggtttgg
     5341 tcgtatcaac tgaaatagga atagctgcgg attttaaagc ttcgataaca ggaataacac
     5401 ggttgagttc ctcatcaaga ctgacgcaag cgctaccagg tcgagaagat tcacccccaa
     5461 tatcaagcaa gtctgcacca tccgctatca atttaatagc atgctcaatc gctttctctg
     5521 atgcagcata tcgtccctga tcggaaaatg aatcgggcgt tacatttatg atacccataa
     5581 tcaatggcgt atctttcttt aaattttctt ttagattaat gtgattattc aaaggaaaaa
     5641 atgaatctag aaatagccct atacctgcaa gcttagatac aaaaaaagaa cagaaaaaaa
     5701 ccgtagatat atcaaagtat atctacggtt gatttaaaag aggattggac gataagaagc
     5761 ttatacttct tgtgcagatc cctcatttgc agctgttgaa gatacggttt tattgtcttc
     5821 tttgggtgct gcagatgatt tagattgtgg tggttgaggt ggacgtggcg gcctattctc
     5881 cataatatct ttaatctgct cgctatcaat agtttcccac tcaagcaatg ctttagtcat
     5941 cgcttcaatt ttatctctat tctcttcgat gagttttcgt gcaagtgcat attgttcatc
     6001 aataatacgg cgaatctctg cgtctacctt ttgcattgta gcttcgctga catttttatg
     6061 tgtagttact gaacgaccca gaaacacttc accttcattt tctccataaa ccatcggacc
     6121 caacacatca gacatacccc attgcgtaac cattcttcgc gtagtttctg tggcacgttc
     6181 aaaatcattt gaagcacctg ttgtcatctg attcataaat atttcttctg caatgcgtcc
     6241 accaaacatt acagaaattg tttgtaaaat ttgatcgcga tccatactga aacgatcctc
     6301 agtcggcaat tgcatcgtaa cgcctaatgc tctcccacga ggaataatcg aaactttatg
     6361 aactggatca gttttgggta acaaataagc aacgactgca tgacctgatt catgataagc
     6421 agtgtttctt ctttcatttt ctggcataac aactgaacgt cgctcagctc ccattagaat
     6481 tttatctttt gctcgttcaa aatcatccat atcaactagg cgcttattag aacgtgcagc
     6541 aaacagtgct gcttcattaa ctaaatttgc caaatccgca ccagacattc caggggttcc
     6601 tcgcgctaaa acatcagcat ggacatctgg tgaaatagga actttgcgca tatgaacttg
     6661 taaaatttgc tcccttcctc ggatatcggg aagtggcaca gtcacttgac ggtcaaaacg
     6721 tcctggacgt aacagcgctg gatcaagtac atcaggacga ttagttgcag caataacaat
     6781 cacccccata ttcccttcaa aaccatccat ttcaacaagg agttgattaa gtgtttgttc
     6841 acgctcatca ttaccaccac caagacctgc tccacgctga cgacctaccg catcaatctc
     6901 atcaatgaaa ataatacatg gggcatgttt tttcgcttgt tcgaacatat ctcttactcg
     6961 agatgcgccc acaccaacaa acatttccac aaaatcagag ccagaaatac taaaaaaagg
     7021 aaccttagct tctccggcaa tcgctctagc caataatgtt ttacctgtac ctgggttgcc
     7081 aaccatcaat acccctctgg gtatacgacc acccaatttt tggaatttgg taggatctct
     7141 cagaaactcg actaactcag ctacctcctc ttttgcttcc tcgcaacctg ccacatcagc
     7201 aaatgtcaca tgattgctat ttttatccaa catccttgct ttgcttttac caaaagagaa
     7261 agcaccgcca ctacgaccgc ctccttgcat ttgccgcata aagaaaatcc aaacagcaat
     7321 taataacagc attggaaacc atgatacaaa aatgctcatc agtatcgaag gttcttcttc
     7381 aggtttagct tcaacgataa cgcccgctct aagtaaatcg ctgactaacc agggatctga
     7441 tggtgcaaat gttataaatt gacgcccatc tgatttagta ccttttaatg tgcgcccatc
     7501 aatagttacc ttcgcgatcc tgccttgatt catttcattg atgaattgtg agtactctat
     7561 cggtgattgc gccgactgtc ttacagtaaa ctgattgaat actgtcataa gaactatagc
     7621 aatcactagc caaatagcca tattttttat taaattattc atagtagatc ctaaatttat
     7681 acattaaagt actagaatgt ttattcctat tattcgtatt aataataaat tcgtttagta
     7741 ttcttttgct agagtaatca cgatttatta acaaatttaa attatttctg tgagataaat
     7801 agcagtctaa cagataattt tacctaatcc taacaaatac aactccttac ttttggaacg
     7861 tgaagctttt ggttttcgta tcataacttt attaaaatga aacctcatat ctcggatgta
     7921 ttgatcaaca taacttcctt gaaaaatttt gacaagaaaa ctccctcctg gtttcaattt
     7981 attattagca aatgctaacg ccaattcagc aagattgata ctgcgtgctt gatcactcat
     8041 tccaattcct gcgagattgg gtgacatatc tgaaaggata agatctgcca gatctcctgc
     8101 caattgattc tcaatcatct cgcaaacttt ttcttcacta aaatcacctt gaataaacgc
     8161 taccccatct agtggaagca tatttaataa atcaagtgca ataatcttac cggatttacc
     8221 gattctttca cggcagactt gagaccaact gccaggggct gcccccaaat caacaacgat
     8281 cattcccgga ctaaaaagct gatcgcgctc agcaatttca atgagtttat aagcagcccg
     8341 cgatcgataa cccgctttct gcgcttgttt aacataaaaa tcattaacgt gctcatgaat
     8401 ccacgctcga ttaactttat ttgatttcat agaagagcta aataattgcc aatgataatc
     8461 accctattaa taaatcatta tgatcatcaa agcgacatct tttaattgat tattttttaa
     8521 agcaaataaa aagtaaggat ttcaagactt acaattgaac tcagggaggt atatttattt
     8581 aggagaataa gagaataaaa taattatcaa tgttattaac gatttcttga agatattaga
     8641 tatactgaat attcaatatt tcgtattccc gaacgcctgc aggggcaacg acttcaacga
     8701 tatctccagc atattttcca atcagcgcac gagcaattgg tgagctaata gaaatttttt
     8761 tctctttgag attcgcctca tcatctccaa caatttgata gattacggtt acatttcccc
     8821 caaaatcttt taattcgact gttgcaccaa aaacacatgc accatcggca tcaatcaatg
     8881 ctggattaat gatttgtgca ttcgcgagtt tactttcgag ttcagcaatt ctaccttcaa
     8941 taaagccttg tctttctttt gcggcatcat attcagcatt ttctgaaaga tctccatgag
     9001 atcgagcttc agcaatcgct gcaataactg caggacgctg cactgttttc atttcgtgaa
     9061 gttcagcacg taatttttca gcacccgcaa cggtcaaagg gattggactc attatgaatt
     9121 ttatccttgt cggtaataag aaatcgaatt actgtattgt ttggtgatgc aacatctgca
     9181 tactataagc atgcaattca cgcatatttg aaatacccat ataagcagcc ctcgcaccgg
     9241 ccattgtggt gtaataagta atttttgcga gtaaagcagc atgtcgaata gagtaagaat
     9301 cttgaatagc tcttcgttta ttagctactg tattgataat caagcaaatt tcaccatttt
     9361 tgatcatatc gactatatga ggccgccctt ccgctacttt atttacttta tgaacattca
     9421 aacccgacgc aatcatcacc tcagcggttc cttgcgttgc ataaattgta aagccaagct
     9481 caataaattt cttagcaagt gtaacggctt ctaacttatc actcgcgcgc acactaataa
     9541 aaatcttacc agagattggt aatttaatcc ctgtcgccaa ctgtgacttt acaaacgctt
     9601 cagcaaaggt gtttccaaca cccatgacct cgccggtaga tttcatttca ggccctaaaa
     9661 tagtatctac accagaaaat ttagaaaatg gaaaaactgc ttctttgacg gagaagaatg
     9721 taggctcaat atctttaaag atggcttgat catataaact tttcttaacc atgcatcgtg
     9781 ccgagatctt tgcaagttgg aggcctgtag ctttcgatac aaagggtacc gtgcgcgaag
     9841 ctcttggatt tacttctagt acatatacaa tatcgttttg aattgcaaat tgcacattca
     9901 ttaatccgat cacattcaat gcacgagcca tcacttctgt ctgccgacga agctcttgct
     9961 gtaattcagt tgataagcta aatggtggaa ttgaacaagc tgagtcacca gaatgaacac
    10021 ccgcttcttc aatatgttcc atgatgccac caatgagtat cgttttccca tcaaaaatcg
    10081 catcgacatc aacctcaatt gcattattta gataatgatc aagtaaaaca ggcgaatcat
    10141 tcgttacttt gaccgcctct tgcatgtatc gctcaaggtc tttctgttcg tgcacaatct
    10201 ccattgcgcg acctcccaat acataactcg gacgcacaac taaagggtag ccaatttcag
    10261 ttgcagcagc caatgccatt tcaacattgc gtgctactcg attcataggc tgcttcaagt
    10321 tatagcgatt aagtaaatcc tgaaaccgtt cacgatcttc tgcacaatcg atcatatcag
    10381 ggcttgtacc aataatcggt acaccaaagc gctcaagatc tctcgctagt tttaaaggcg
    10441 tttgcccacc atattgaaca ataactccat tcggcttttc tacagcaacg atattcagga
    10501 catcttctag tgtaagcggc tcaaaatata aacgatctga tatatcgtaa tccgttgaaa
    10561 cagtttctgg attacagttg accatgattg tttcatagcc gtcttcacgt aatgcgagtg
    10621 acgcatggac acagcagtaa tcgaattcta ttccctgccc tatgcgattc ggtcctccac
    10681 ccaacaccat aatcttcttt ctagtgctgg gattagcttc acattcctct tcgtaagtag
    10741 aataaagata ggctgtatct gtggaaaatt cagcagcaca ggtatcgaca cgcttatata
    10801 ccgggtatat tttttcattt aggcgacggg tacgaatcgc taattcactt gaattgagta
    10861 gtttggccaa tctacgatct gagaatccac tcctttttaa ttggcgcaat aagtccgtat
    10921 caatagaatc cactgtacta tcagtcaaag attgctcttg ctggataaga ttttctattt
    10981 gatgcaaaaa ccaggggtca atgtgtgtta attgataaat ttcatcaaaa gccatgccat
    11041 gtcgaaatgc atcggctaca taccaaatcc gctcaggacc aggatgtcct aattcagccg
    11101 ctatcaaatc taaatcggat gatttttcat ctaaaccatc aacgccaatc tcaagtccac
    11161 gcaaagcttt ctgaaatgac tcttgaaatg tgcgcccgat cgccataacc tcacccactg
    11221 atttcatctg agtcgttagt cgatcattgg cttgaggaaa tttctcaaat gcaaatcgag
    11281 ggattttagt aacgacgtaa tcgattgtgg gctcaaaaga agcaggcgta acaccgccgg
    11341 taatatcatt actgagttca ttcaatgtat aacctacagc cagtttggca gcaatcttgg
    11401 caataggaaa tccagtggct ttagaagcaa gcgcagaaga tcgggaaaca cgtggattca
    11461 tttcgatcac tagcagacga ccatcagcag ggttaatcgc gaattgaaca tttgatccgc
    11521 ccgtttcaac gccaatttca cgtaataccg caatagaggc attacgcatg atttggtatt
    11581 ctttatcagt gagtgtctgg gccggcgcga ccgtaatcga atcaccggta tgaataccca
    11641 tcggatccaa gttttcgatc gagcaaatga taatgcaatt atcttgctta tcacgaatta
    11701 cctccatttc aaactctttc cagccaatta cagattcttc aatcagtaat tcgcgagtgg
    11761 gtgaggcatc caatccgcgt tcacaaattt ctataaattc ttgtcgatta tacgcaattc
    11821 ctccacccgt tccgccgagc gtaaaagaag ggcgaataat tactggataa ccaatcatcg
    11881 cctgtacttg cagtgcttct tccatgctgt gagcaatcgc agagcgagca gagctcaatc
    11941 cgatatgagc catcgcttgt ttaaatttct cacgatcttc cgctttatca atcgcttctc
    12001 gcgatgcgcc aattaactca actccatatt tatcgagcac tccatattta actaaatcaa
    12061 gcgcacaatt tagagctgtt tgtccaccca tggttggtaa gagtgcatct ggacgctcga
    12121 tatcgataat tttctcaagc atgggccagg ttataggctc gatataggtc gcatccgcca
    12181 tttctggatc tgtcatgatc gtcgctggat ttgaattgac cagtacaatt cgataacctt
    12241 cttcccgtag cgcggtacaa gcttgaacac ccgagtaatc aaactcacaa gcttgaccaa
    12301 taatgatagg ccctgctcca atcacaagaa tcgatttaat atcagtacgt tttggcattt
    12361 atattgcagc aacaaaggct tagtttaagt cttacttcgc aacgtgactc tgttgcttct
    12421 tatattgttt gattcgatcg ataaaacgat caaatagata
//
LOCUS       DAOQLJ010000064        12237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_1991_length_12237_cov_5.966426, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000064 DAOQLJ010000000
VERSION     DAOQLJ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 12237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12237
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1991_length_12237_cov_5.966426"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /gene="rplL"
                     /locus_tag="PK522_06015"
     CDS             <1..259
                     /gene="rplL"
                     /locus_tag="PK522_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNC41323.1"
                     /translation="AVAAPAAAAASAAEEQTEFSVVLTSAGDNKVNVIKVVRAVTGLG
                     LKEAKDLVDGAPKVVKEGIAKADAEAIRKQFTEAGAACDIK"
     gene            433..4506
                     /gene="rpoB"
                     /locus_tag="PK522_06020"
     CDS             433..4506
                     /gene="rpoB"
                     /locus_tag="PK522_06020"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNC41324.1"
                     /translation="MDYSFTEKKRIRKSFAKRASVLSIPFLLATQLESYTSFLQANKL
                     ANSRKNIGLQAAFNSIFPIESHSKNARLDFISYNLGHPPFDVKECQQRGLTYASPLRA
                     KVRLTILDKESSKPTVKEVKEQEVYMGEIPLMTETGSFVINGTERVIVSQLHRSPGVF
                     FEHDRGKTHSSGKLLFSARIIPYRGSWLDFEFDSKDYVYFRIDRRRKMPVTTLLKAMG
                     CTPEQILADFFVFDHFHFSKNKILFDLVPERLRGEVASFDIVSSSGDIIIQKDKRITA
                     KHVRDLQQSQIEKIEVPEDFLLGKILAKNIIDQKTGEIIALANDEITETILEKIRVAG
                     IKAVTTLYINDLNQGPYISLTLRTDEVADQFSAQVAIYRMMRPGEPPTEEAVIALFNG
                     LFFSAERYDLSVVGRMKFNRRVGRAELEGLPTLTHQDIIDVIKILVELRNGRGEIDDI
                     DHLGNRRVRSVGELAENQFRAGLARVERAVKERLSQAESENLMPHDLINAKPVSAAAR
                     EFFGSSQLSQFMDQTNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHTTHYGRVCPI
                     ETPEGPNIGLINSLALYAQTNEYGFIETPYRKVVEGTVTDQIVFLSAIEESQYVIAQA
                     NAKLDSDGRFIDEIVSCRHRNEFTLSPKDQIDYVDVAPAQIVSVAASLIPFLEHDDAN
                     RALMGSNMQRQAVPCLRAEKPLVGTGIERVVAEHSGTAVRAIRGGVVDFVDANRIVIR
                     VHDEETLAGDVGVDIYNLIKYTRSNQSTNINQRPLVKIGDVIERNNVIADGASTDMGE
                     LALGQNMLVAFMPWNGYNFEDSILISERVVAEDRFTSIHIEELSVVSRDTKLGTEEIT
                     ADIPNLSERQLSRLDESGIVYIGAEVEVGDVLVGKVTPRGETQLTPEEKLLRAIFGEK
                     ASDVKDTSLRVPSGISGTVIDVQVFTREGVQRDKRSKKIINDELDRYKKDLADQMRIV
                     ETDVFKRLERLLIGKVVTGGPKKLAKGTAITKEYLDSIDLHHWFDIRLADEDISRQLE
                     QMKEGLEQKQKLFDSAFEEKRKKLTQGDELPPGVQKMVKVYVAVKRRLQPGDKMAGRH
                     GNKGVISKIVPIEDMPYMADGTPVDVVLNPLGVPSRMNVGQILEVHLGWASKGLGEKI
                     GALLQQEKRVAKLRPFLETIYNSTGMKEDIASLNDDELIELARNLVGGVPFATPVFDG
                     ATEAEIKAMLELADLPKTGQVTLYDGRTGEAFDRSVTVGYMHVLKLHHLVDDKMHARS
                     TGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVGGRTKVY
                     ESIVKGDHKIDAGMPESFNVLVKEIRSLAMDIDLEQY"
     gene            4560..8792
                     /gene="rpoC"
                     /locus_tag="PK522_06025"
     CDS             4560..8792
                     /gene="rpoC"
                     /locus_tag="PK522_06025"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNC41325.1"
                     /translation="MRALLDLFKQVTQKEEFDSIKIGLASPEKIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTLSKVRRERM
                     GHIELASPVAHIWFLKSLPSRLGLVLDMTLRDIERVLYFEAYVVTDPGMTPLTQKQLL
                     TEEDYLNKTEEYGDDFRAAIGAEGIRDLLNSMNVQVEIDSLRLEMQSTNSETKIKKIA
                     KRLKVLEAFNKSGLKPEWMILTVLPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELRAPEIIVRNEKRMLQESVDSLLDNGRRGKAMTGANKRPLKSLADMIKGK
                     GGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKKMALELFKPFIFNKLEVMGIAS
                     TIKAAKREVENETPVVWDILEEVIREHPVMLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAFNADFDGDQMAVHIPLSLEAQMECRTLMMSTNNILSPANGEPIIVPSQDIVL
                     GLYYMTREKMYAQGDGMFFQDMVEALRAYENGAVELNAKIQVRIKEIDMVSKAEKIQR
                     YETTVGRALLSEVLPEGLSFSLVNKVLKKKEISKLINACFRLCGLRKTVIFADKLMNL
                     GFTYATRGGISICSDDLRIPVQKNDIIKSAEAEVQEIATQYTSGLVTQGERYNKVVDI
                     WGRAGDQVAKAMMDQLSVENIYDSSSNEIKKDDHGKPLMQESFNSIYMMADSGARGSA
                     AQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTAN
                     SGYLTRRLVDVTQDLVITENDCGTEDGVVMKALIEGGEVIEALKERLLGRVTVQEVIN
                     PDNQTVLYPAGFLLDENAVEQIEFYGVDEVKVRTPLTCETRYGLCAKCYGRDLGRGSL
                     VDVGEAVGVIAAQSIGEPGTQLTMRTFHIGGAASRTAVASQIESKSNGVIQYSNSIRY
                     VKNSRNELIIISRSGEVIISDDNGREKERHKIPYGATLLVHDGDAVKIGKVLASWDPH
                     TRPIITEYSGKVRFENVEEGVTVAKQIDDITGLSTLVVIDPKRRSTSSQSKGLRPLVK
                     FLDENEKEIKVAGTDVSVSITFQIGSIITVRNGQQVSVGEVLARIPQETSKTRDITGG
                     LPRVAELFEARSPKDAGLLAEVTGVVSFGKDTKGKQRLVITDLDNVSHEYLIPKDKHV
                     MAHDGQVVNKGEIIVDGPADPQDILRLQGVEVLARYITDEVQDVYRLQGVKINDKHIE
                     VIVRQMLRRVQITEAGDTSFIPGEQVERADVLIENEKVLAEGKIPAAYNYILLGITKA
                     SLSTDSFISAASFQETTRVLTEAAIMGKRDELRGLKENVIVGRLIPGGTGLSFHNIRK
                     KQKAKSSTEPNLLAEKSNGEVDNVTPINDNMA"
     gene            9093..10262
                     /locus_tag="PK522_06030"
     CDS             9093..10262
                     /locus_tag="PK522_06030"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNC41326.1"
                     /translation="MSLREISKSNFFQKVKIDRAHIDVLFQSLKNRGYRLVGPKISEG
                     AIIYDFLDVVDELPIGWTDEQEAGTYRIKQREDQALFGYNLGPHSWKKFLFSARKRVW
                     QGQKTSEGSFKTVQEMVAQSQPYAFIGVRSCELHAIQIQDRVFMNGQYQDTEYTQKRA
                     DNFIVAVNCGQAGKTCFCTSLNTGPRVSQGYDLALTEILDGGTHYFVVESGSERGLAV
                     LQELPCQSVGNNDLEAVDKCIEKAVGQMGRAIDTTDIKAFLYRNYDNKHWRKLEERCL
                     TCANCTMVCPTCFCSKIEDTTDLTGEYAERWQTWDSCFTMDFSYTHGGAVRASTQSRY
                     RQWLVHKLASWIDQFGTSGCVGCGRCITWCPVGIDLTEEIKALKQAEREAKNGEN"
     gene            10249..10722
                     /locus_tag="PK522_06035"
     CDS             10249..10722
                     /locus_tag="PK522_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNC41327.1"
                     /translation="MVKIRNIEALLLESPFFQSFPQDHLRLLAECSEIVHFNENELLL
                     TEGEKADYFYLILSGSVAIESHYPGREALIVSKVVTQGIVGFSWLFPPFRNQFDARAA
                     TRGSAIKLNGSCLLPIIESDHALGYYLLKGFSAIMLQRMQSARRQMLDVFGQSKN"
     gene            10727..11560
                     /locus_tag="PK522_06040"
     CDS             10727..11560
                     /locus_tag="PK522_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HNC41328.1"
                     /translation="MSLQALLNNPMEPTPFRILRVKQELKDCFTLILDCKQTPFHFAP
                     GQFNMLYIFGHGEVPISMSGDPNQPELLVHTIRNVGSITAALEQLREGDFVGVRGPFG
                     SPWPLSEAKGKDIVILAGGLGLAPLRPVIYHTLEHQSDYKKITLCYGARNPEAILFSD
                     ELEQWTHRIAVELTVDSAGPGWSGHVGVITDRLQQQAIVLTNTIAFVCGPEIMMRYSA
                     YALIDNGLSADRIYISMERNMKCAMGLCGRCQFGPYFICQDGPVFSFDKVEQLLKIRE
                     A"
     gene            11611..>12237
                     /locus_tag="PK522_06045"
     CDS             11611..>12237
                     /locus_tag="PK522_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfhydrogenase subunit delta"
                     /protein_id="HNC41329.1"
                     /translation="MEPIEKKKPKLAVWKFASCDGCQLTLLNCEDELLTLTENIQIAN
                     FVEASREVLPGPYDLSLVEGSITTPQDLTRIQQIRAQSNYLVVIGACATAGGIQALRN
                     FKDVKEFINIVYAHPSYIDTLATSTAIAEHVNVDFELRGCPINKQQLLEVINAFLHRR
                     KPNIPSYSVCIECKRRGNPCVMVMGKPCLGPVTHAGCGAICPSYQRGCY"
BASE COUNT         3720 a         2114 c         2852 g         3551 t
ORIGIN      
        1 agctgttgct gcaccggctg cagcagctgc atcagctgct gaagaacaaa cagaattttc
       61 tgtcgttctg acttcagctg gagataataa agttaatgta attaaagtag ttcgcgctgt
      121 gacgggtctt ggattgaagg aagctaaaga tttagttgac ggagcaccaa aagttgttaa
      181 agaaggaatt gctaaagctg atgcagaggc gattcgcaaa caatttacag aagctggtgc
      241 ggcatgtgat attaaataat agcgataaca gttaatacta aattaatagt tttggctgat
      301 agcaatcgct ggctatcagc ctttgctcca tttgactaat gtaaattcag tcttgatagt
      361 tgaggtaacc actttttagt tagattcaat aagcggtatt tacattgaat cttctgttcc
      421 tggagaaagt tgatggacta ttcgttcacc gagaaaaaac gcatacgtaa gagttttgca
      481 aagcgagcaa gtgtattatc cataccattt cttcttgcaa ctcagctgga atcctataca
      541 tcgtttttac aagctaataa gctagcaaat tctcgaaaaa atatcggtct acaagcagca
      601 tttaattcga tttttcctat tgaaagtcat tctaaaaatg ctcgtttaga tttcattagc
      661 tacaatttag gacatccacc ctttgatgtg aaggaatgtc agcaaagggg gttaacttat
      721 gcttctcctt taagagcaaa agtccggttg actattcttg ataaagaatc ttcaaaacca
      781 actgtaaaag aagttaaaga gcaagaagtg tatatgggag agattcctct tatgaccgaa
      841 acagggtcat ttgtcataaa tggtacagag cgagttatcg tgtcccagct tcatcgttct
      901 cctggtgtat tttttgagca tgaccgaggg aaaacgcact catcaggtaa gttacttttt
      961 tctgcaagaa ttattcccta tcgcggctct tggcttgatt ttgagttcga ttctaaagat
     1021 tatgtttatt ttcgtataga tcgtagacgt aaaatgcctg tgactacgct attaaaagcc
     1081 atgggctgta ctcctgagca aattctcgca gatttttttg tttttgatca ctttcatttt
     1141 tctaaaaaca aaattttgtt tgaccttgtt cccgagagac ttcgcggtga ggtggcaagc
     1201 tttgacatcg tatctagcag tggcgatata ataatccaaa aagataagcg aatcacggct
     1261 aaacacgtta gggatttaca gcaatcccaa atagaaaaaa ttgaagttcc agaagatttt
     1321 ttgctcggga aaattctagc taaaaatatt atagatcaaa aaactggtga aatcattgcg
     1381 ctagcaaacg atgagattac tgaaacaata ctagaaaaaa taagagttgc tgggattaaa
     1441 gcagttacaa cgctttatat aaatgatctc aatcaaggac cttatatttc cctaacttta
     1501 cgaacagatg aagtcgctga tcagttttct gctcaagtag ctatctatcg catgatgcgc
     1561 cctggtgagc caccaactga agaagcggtt attgctctgt ttaatggttt atttttctca
     1621 gcagaacgtt acgatctatc agtagtgggt cgaatgaaat tcaatcgccg tgtggggaga
     1681 gcagaattag aaggactacc tacgttaact caccaagata ttattgatgt aatcaaaatc
     1741 ttagtagagc ttcgaaatgg aagaggtgaa attgatgata tcgatcactt gggaaatcgt
     1801 agagtgcggt cagttgggga attagcagaa aaccaattta gagcaggcct ggcgcgagtt
     1861 gaaagagctg ttaaagagcg tttaagccag gcggaatcag agaatttaat gcctcatgat
     1921 ttaattaatg ctaaaccggt atctgctgcg gcccgtgaat tttttggttc aagccaatta
     1981 tcacaattca tggatcaaac gaatccactt tctgaagtaa cacataagcg tcgtatatcg
     2041 gcgctagggc cgggtggctt aacacgtgag cgtgctggat ttgaagttag agacgtgcat
     2101 acaacccatt atgggcgtgt ttgtccaatt gaaactccag aaggaccaaa tataggattg
     2161 attaattctc ttgcgcttta tgctcaaact aatgaatatg gttttattga aacaccttac
     2221 agaaaagttg tcgaaggaac tgtaacagat caaattgtat ttctttctgc aattgaagag
     2281 agtcagtacg ttattgcaca agcgaatgca aagttagatt ctgatgggcg atttattgat
     2341 gaaattgttt cgtgtcgtca taggaacgaa ttcacactct ctccaaaaga tcaaattgat
     2401 tatgtcgacg ttgcgcctgc acagattgta tccgtagctg cttcattaat accttttttg
     2461 gagcatgacg atgcaaatcg tgcattaatg ggttcaaata tgcagcgtca agctgtacct
     2521 tgtttgcgtg cagagaaacc actcgtagga actgggatag agcgcgtggt tgctgagcat
     2581 tctggaactg ctgtaagggc aataagaggt ggggttgttg attttgttga cgcaaataga
     2641 atagtcattc gtgttcatga tgaagaaacc ctcgcgggcg atgtcggggt ggatatctat
     2701 aacttgatca aatatacccg atccaatcaa agcacaaata ttaatcaacg gccgcttgtt
     2761 aagattggtg atgtaatcga acgtaataat gtcattgcag atggcgcatc aaccgatatg
     2821 ggggagcttg cattagggca aaacatgctc gttgcattta tgccatggaa tggttacaac
     2881 tttgaagact cgattcttat ttctgaacga gttgtagctg aggatcgctt tacatcaatt
     2941 catatcgaag aactttcggt agtgagtcgc gatacaaagc ttggaactga agaaattact
     3001 gctgatatac ctaatttatc cgaacgtcag ctatcacggc ttgatgagtc agggattgta
     3061 tatattggcg ctgaagtaga ggtgggtgac gttttggttg gtaaagttac acctagaggc
     3121 gaaacgcagt taacaccaga agaaaaatta ctgcgcgcta tttttggaga aaaagcctca
     3181 gatgttaagg atacgtcctt acgagtacca tctggaatct ctggaacggt tatcgatgta
     3241 caagttttca ccagagaagg ggtacagcgt gataagcgtt ccaaaaaaat aattaatgat
     3301 gaattagatc ggtacaaaaa agatctggct gatcaaatgc gcatcgttga aacagatgta
     3361 tttaagcgcc ttgaaagatt acttattgga aaagtagtta caggtggacc caaaaaatta
     3421 gcaaaaggta cagcaattac caaagagtat ctcgatagta ttgatttgca tcactggttt
     3481 gatattcgat tggctgatga agatataagc cggcaattag aacagatgaa agaagggtta
     3541 gaacaaaaac aaaaattgtt tgatagtgcg tttgaagaaa aacgaaagaa attgactcaa
     3601 ggtgatgaat taccgccagg tgttcaaaaa atggttaagg tttatgtggc tgttaagagg
     3661 cggctacaac ccggcgataa gatggctgga aggcatggaa ataaaggggt tatttcgaaa
     3721 atcgttccaa tagaagatat gccttacatg gcagacggta cgccagtcga tgtcgtattg
     3781 aatccactgg gtgtgccatc gcgaatgaat gtcggtcaaa tattagaagt acatctgggg
     3841 tgggcatcta aaggtcttgg tgagaaaatt ggagcattat tacaacaaga aaaaagagtc
     3901 gctaagttga ggccattcct cgaaacaata tataacagta ctggtatgaa agaagatatc
     3961 gcatcactga acgatgacga gctaattgaa ttagcgagga atttagtagg aggtgttcca
     4021 tttgctacac ctgttttcga tggagcaacc gaagctgaga ttaaagccat gctagagctt
     4081 gctgacttac ctaaaacagg tcaagtaact ctttacgatg gtcgaacagg agaagcgttt
     4141 gatcgctcgg ttacggttgg ttatatgcat gtattaaaac ttcatcatct agttgatgat
     4201 aagatgcatg caagatcaac agggccttat agcttggtta cgcaacaacc actaggtggt
     4261 aaagctcagt ttggggggca acgttttgga gaaatggagg tatgggccct agaagcctat
     4321 ggggcagcat atactttaca ggaaatgtta actgtaaaat cggatgatgt tgggggtaga
     4381 acaaaagtat atgaaagtat agttaaaggg gaccataaaa tcgatgcagg aatgcctgag
     4441 tcatttaatg tactcgtaaa agagattagg tctctggcaa tggatatcga tttagagcaa
     4501 tactaataag aaagccagac tagcatttta tataacacca ttaggataca ggagtaaaaa
     4561 tgagagcatt gcttgattta tttaaacaag taactcaaaa agaggagttt gattcgatca
     4621 agattggtct tgcctctcca gagaaaatac gttcttggtc atatggtgaa gtaaaaaaac
     4681 ctgaaacgat taattatcgt acgttcaagc ctgaaagaga tggtttgttc tgtgccaaaa
     4741 tttttggtcc tgttaaagac tatgaatgtt tatgtggcaa atataaacga ttaaagcaca
     4801 gaggggttat atgtgaaaag tgcggcgtcg aagtaacatt atcaaaagtt aggcgtgaac
     4861 gcatggggca tattgaacta gcgtcccctg tcgcgcatat ctggttctta aaatccttac
     4921 cttctcgatt aggtttagta ttagatatga ccttaaggga tattgagcga gtgctttatt
     4981 ttgaagcata cgttgtgact gatcctggta tgacaccctt gacacaaaaa caattattaa
     5041 cagaagaaga ttatttaaat aaaacagaag aatatggtga tgattttcgt gctgctattg
     5101 gagctgaagg tattcgagat ttattaaata gcatgaatgt tcaagtggag attgattcac
     5161 tccggcttga gatgcagagt acaaactctg aaactaaaat caagaaaatt gcaaaaagat
     5221 taaaagtact tgaagcattt aataaatcgg gattgaagcc tgaatggatg attttgacgg
     5281 tcttgccggt attaccacct gagttaagac cgcttgttcc tctggatgga ggtcgttttg
     5341 ctacctcaga cctcaatgat ttatatagac gagtcattaa cagaaataat cgcttgaagc
     5401 gattacttga gcttcgagcg ccagaaatca ttgtcagaaa tgaaaaaaga atgttgcaag
     5461 aatcagttga ttccttgcta gataatggtc gtcgtggtaa agcaatgacg ggagctaata
     5521 agcgcccact taaatccctg gcagatatga ttaagggtaa aggaggacgt ttccgtcaaa
     5581 atttacttgg taaacgagtc gattattctg gtcgatcagt cattgttgtt ggtcctcagc
     5641 tcaaacttca tcaatgcggg ttaccgaaga aaatggcgct agagctattt aagcccttca
     5701 tttttaataa gctagaagta atggggattg ctagcacgat aaaagctgct aagcgcgaag
     5761 tggaaaatga aactcctgtg gtttgggata tcttagaaga agttattcgc gagcatcctg
     5821 taatgctaaa tcgcgcacca acgcttcatc gcctcggtat tcaagctttt gaacctgtac
     5881 tgatagaagg gaaagcgatt caattacatc cgctagtctg tgcagcattt aatgctgact
     5941 ttgatggcga ccaaatggct gttcatattc cactgtcact agaagcgcag atggagtgtc
     6001 gaacgttaat gatgtcgact aataacattc tttcgccggc aaacggagag cccataattg
     6061 tcccatctca agatatcgtt ctgggccttt attacatgac tcgtgaaaaa atgtatgcac
     6121 agggtgacgg gatgtttttt caggatatgg tagaagccct tcgtgcatac gaaaatggtg
     6181 cagtagagct gaatgccaaa attcaggtta gaattaaaga aatcgatatg gtttctaaag
     6241 cagaaaaaat ccagcgctat gaaacgacgg ttggtcgtgc attgttatcc gaggttttac
     6301 cagagggtct ctctttttca ctcgtaaata aggtactcaa gaagaaagag atatcgaagc
     6361 ttattaacgc ctgcttccga ttatgtggat tacgtaaaac agtcattttt gctgataagc
     6421 tcatgaattt aggttttact tatgcaactc gtggcggtat ctcaatttgt tcagatgatt
     6481 tacggattcc tgtacaaaaa aatgacatta ttaaatctgc tgaagcagaa gtacaagaga
     6541 tagcaaccca atatacttca ggacttgtta ctcaaggtga aagatacaat aaagtagtcg
     6601 atatttgggg acgtgctggt gatcaagttg ccaaagcgat gatggatcag ttgagcgtag
     6661 aaaatatcta tgattcatct tcaaacgaaa ttaagaaaga cgatcatggt aaaccattga
     6721 tgcaagaatc atttaactct atctatatga tggctgattc gggtgcaagg ggttcagctg
     6781 ctcaaattag acaactggcg ggtatgcggg ggctaatggc aaaaccagat ggatcaatca
     6841 ttgaaacacc gatcactgca aactttcgtg aagggttgaa tgtattacag tacttcattt
     6901 ctacgcatgg tgcacgtaaa gggctagcag acactgcatt aaaaacagca aattcaggct
     6961 atttaacacg acgtcttgtc gatgtgaccc aagatttagt gattacagag aatgattgtg
     7021 gcactgaaga tggtgttgtt atgaaagcct tgatagaagg tggtgaagtc attgaggcac
     7081 tgaaagaaag gttattaggt cgtgtgacag tacaagaagt aatcaaccca gacaatcaaa
     7141 cggtattata tcccgcaggt tttctgcttg atgagaatgc ggttgagcag attgagtttt
     7201 atggtgttga tgaagttaaa gtgcgaacac cgcttacctg tgaaacccgt tatggattat
     7261 gcgctaagtg ttatggtcgt gacttgggaa gaggttctct cgttgatgtg ggtgaagccg
     7321 ttggtgtcat tgcagcgcaa tcgattggag agcctggtac acagctaacc atgagaactt
     7381 tccacattgg gggagcagct tcgagaactg cagtggcaag tcaaatcgaa agtaaatcaa
     7441 atggtgtgat tcaatattca aactctatcc gttatgtaaa aaattctcgt aatgaattaa
     7501 ttattatttc gcggagtggt gaagtgatca tttctgatga taatggtcgt gagaaagaac
     7561 gccataaaat tccttatgga gctacattac tcgttcatga tggtgatgcc gtgaaaattg
     7621 gtaaggtact tgcgtcatgg gatccacata cacggccgat cattactgag tatagtggga
     7681 aggttcgttt cgagaatgta gaagaaggtg taacagtagc taagcaaatt gatgatatca
     7741 ccggtttgtc cacacttgtg gttattgatc cgaaacgccg gagtacttca agccaatcca
     7801 aaggattgcg accgttagtt aaattcttgg atgaaaatga gaaagaaatt aaagtggccg
     7861 gtactgacgt atcagtaagt attacattcc aaattggatc tattattacg gtacgtaatg
     7921 gtcagcaagt atcagttggt gaagttctag cacgaattcc gcaagaaact tcaaaaacgc
     7981 gtgatattac tggtggttta ccacgtgttg ctgaactatt tgaagctcgt tcgcccaaag
     8041 atgctggctt gctggcggaa gtgactggtg ttgtgtcctt tggtaaagat accaaaggta
     8101 agcaacgcct ggttattaca gatcttgata acgtttctca tgagtactta atccctaaag
     8161 ataagcatgt catggctcat gatggtcaag tcgtaaataa aggtgaaatt attgtggatg
     8221 gtccggctga tccgcaagat attttacgat tacagggtgt agaggtatta gcgcgctata
     8281 ttacggatga agtacaggat gtttatcgat tacaaggtgt aaaaattaac gataagcata
     8341 ttgaagtaat cgttagacaa atgctacgtc gcgtccagat tactgaagca ggggatactt
     8401 catttattcc tggggaacaa gttgaaagag cggatgtatt gattgagaat gaaaaagtat
     8461 tggctgaagg gaaaattcct gcagcttata actatatatt actaggtatc actaaagctt
     8521 cgctttcgac tgactcattc atttcggcag cttccttcca agaaacgact cgtgtattga
     8581 ctgaagccgc aattatgggt aaacgggatg agttgcgagg tttaaaagag aatgtgatcg
     8641 ttggtaggct tatccctggt ggaacaggat tgtctttcca taatattcgt aagaaacaaa
     8701 aagcaaaatc ttctaccgag cctaatttat tagcagaaaa gagtaatgga gaagttgata
     8761 atgttactcc aattaacgat aacatggcct aagtaaattt ttgactatta tctgcggtag
     8821 ttttttaagt tgtacatagc aaatcaataa aattccaccc atattttcat tagaaaaatt
     8881 tttgatgaaa atgtgggttt gctccaaaaa gtctacgatt ttccattaat gacaggttac
     8941 caatatacct tgtcagtttt tgcagctatc tttaataaat tccctttttt tgaatgatca
     9001 tctaacatga tcaaaaagaa tcttgcagtg ttatcaagga ttggtaactc tctgtcatgg
     9061 ttcagtaaaa taaatctcat aaaattttgc agatgagctt gcgggaaatt tcaaaatcta
     9121 atttttttca aaaagttaaa attgatcggg cccatattga tgtacttttt caatcgttaa
     9181 agaatcgtgg ctatcgcttg gttggcccaa agatcagtga aggcgcaatc atttacgatt
     9241 tcctggatgt tgttgatgag ctgccgatcg gatggacaga cgagcaagaa gctggaactt
     9301 atcgtattaa gcaacgagaa gatcaagctt tatttggcta taacttaggg cctcattctt
     9361 ggaaaaaatt tttgttttct gcgcgtaaaa gagtctggca aggacagaaa acatctgaag
     9421 gtagtttcaa aacggtacag gaaatggtag cgcaatctca gccctatgca tttattggtg
     9481 ttcgatcttg tgaattacat gcgatccaaa tccaggatcg ggtttttatg aacggtcagt
     9541 atcaggatac tgaatatacg cagaaacggg cagataattt cattgtagcg gttaactgcg
     9601 ggcaagccgg aaagacttgt ttttgtactt ccctaaatac cggccctaga gtaagtcaag
     9661 gttatgatct tgcattaacc gaaattcttg atggtggtac acattatttt gtggtggaat
     9721 ctggcagtga gcgaggatta gccgtgttgc aggaattacc ctgtcaatct gtcggtaata
     9781 acgatttaga agcggttgac aaatgtatcg agaaagcggt cgggcaaatg ggtcgtgcta
     9841 tcgataccac cgatatcaaa gcgtttcttt atcgaaatta cgataacaag cactggcgga
     9901 aattagaaga aagatgcctg acttgtgcaa actgtaccat ggtttgtcct acctgctttt
     9961 gttcaaaaat tgaagatacg acggatttaa ccggagaata tgctgaacgg tggcaaacct
    10021 gggattcttg ttttacgatg gatttctcat atacccacgg tggcgctgtg cgtgcttcaa
    10081 ctcaatcacg ttatcgtcaa tggttagtgc acaaactcgc aagttggatt gatcagtttg
    10141 ggacatctgg ctgtgttggt tgtggtcgtt gcattacgtg gtgtccagtt gggatcgatt
    10201 taacggaaga aataaaagca ctgaaacaag ctgagcgtga agcgaaaaat ggtgaaaatt
    10261 agaaatatag aagcattatt actggaatcg cctttttttc aatcctttcc acaggatcat
    10321 ctcagattac tcgctgaatg cagtgaaatc gttcatttca atgaaaatga attgttattg
    10381 accgagggag agaaagcaga ttatttctat ctcattttga gtggaagtgt tgcgatcgag
    10441 agtcattacc caggccggga agcgctgatt gtttcaaaag tggtgacaca gggtatcgtt
    10501 ggtttttcct ggttgtttcc cccttttcgt aatcaattcg atgcgcgtgc tgcgactcgc
    10561 gggtctgcaa taaagctcaa tgggtcctgc ttgttgccta tcatcgaaag tgatcatgca
    10621 ttgggatatt atttattaaa aggcttttca gcgatcatgc tgcagcgaat gcaatcggct
    10681 cgtcggcaaa tgctggacgt ttttggtcaa tcaaaaaact agctcgatgt cactacaagc
    10741 tttactcaat aatccaatgg agccgactcc attccgaatt ctacgagtaa agcaagaatt
    10801 aaaggattgt tttacgctta tattggattg taaacaaacg ccttttcact ttgcacccgg
    10861 tcaattcaat atgctttata tatttggcca tggtgaagtt ccgatttcca tgagtggaga
    10921 tcctaatcaa cccgagttac ttgtgcatac catccggaat gtagggtcga ttactgcagc
    10981 gttagaacag ctaagggaag gtgattttgt tggggttcgg ggtccctttg ggagtccatg
    11041 gccactttct gaagctaaag gaaaagatat tgttatttta gcgggtggat tgggtcttgc
    11101 acctttgcgg ccagttattt atcacaccct ggaacatcag agtgattata aaaaaataac
    11161 gctatgctat ggtgcacgta atcctgaagc gatattattt tcggatgagc ttgaacaatg
    11221 gacccatcga atcgcggtgg agttaacggt tgatagcgct ggaccaggat ggtcggggca
    11281 tgtaggggtg attaccgata gattgcaaca acaagcgatt gttttaacga ataccatcgc
    11341 ttttgtttgt ggcccagaaa tcatgatgcg ctatagtgcc tatgctttaa tcgataacgg
    11401 tcttagtgcc gatcgcatct atatttcgat ggaacgtaat atgaaatgtg caatgggact
    11461 ctgtggtcgc tgtcaatttg ggccttattt tatctgtcag gatggaccgg tattctcatt
    11521 tgataaagta gaacaactgc ttaaaatccg agaagcgtag gcttgagtgc ttggtttttt
    11581 gatagtttaa atcgccagag aaaacatgta atggaaccga ttgaaaaaaa gaagccaaaa
    11641 cttgctgttt ggaagtttgc ttcgtgtgat gggtgtcaac taacgcttct aaattgtgaa
    11701 gatgagttgc tcacactaac ggaaaacatt caaattgcga attttgttga agcttcccgt
    11761 gaagtgctgc cgggacctta tgatttgtcc ttagtagaag gatcaattac tacaccgcaa
    11821 gatttaacac ggattcagca aattcgtgcg cagtcgaact acttggtggt gatcggtgct
    11881 tgtgcaacag ccggcggaat tcaagcgctg cgtaatttca aagatgtaaa ggagtttatc
    11941 aatatcgttt acgcgcatcc atcctatatt gatactttgg cgacttccac tgccattgcg
    12001 gaacatgtca atgtcgattt tgagcttcgt ggttgcccca ttaacaaaca gcaattgctg
    12061 gaagtaatta atgcatttct gcatcgacga aaacccaata ttcccagtta tagtgtttgt
    12121 atcgaatgca aacggcgagg gaatccttgt gtgatggtga tgggtaagcc ctgtcttggg
    12181 ccggttactc acgcagggtg tggtgcaatc tgtccttctt atcagcgtgg ttgctat
//
LOCUS       DAOQLJ010000065        11836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2081_length_11836_cov_6.120448, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000065 DAOQLJ010000000
VERSION     DAOQLJ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 11836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11836
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2081_length_11836_cov_6.120448"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /gene="rsmH"
                     /locus_tag="PK522_06050"
     CDS             <1..563
                     /gene="rsmH"
                     /locus_tag="PK522_06050"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNC41330.1"
                     /translation="RFSLPKLLQRSCARVGIRNYGEERHARKIARAVVMARAKYPIST
                     TFQLAEIITEVMRTGGYQHGGKQHPATRTFQAIRIHLNQELEELSLVLPQCVELLNPK
                     GRLVVISFHSLEDRIVKRFMRAKANPEGNLPRKIPLTSKEVQELIHREVLLIGDALKP
                     TSIEIARNPRARSAVMRVAEKLKNGR"
     gene            565..840
                     /gene="ftsL"
                     /locus_tag="PK522_06055"
     CDS             565..840
                     /gene="ftsL"
                     /locus_tag="PK522_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNC41331.1"
                     /translation="MIKLNILLVLILIFSGLGVVTAQHETRKLVISLEKEHNLTDQLA
                     IERNQLLIEQRTWAMPGYVEKIAKNKLDMIAPSSSHITVITPDHVLN"
     gene            868..2598
                     /locus_tag="PK522_06060"
     CDS             868..2598
                     /locus_tag="PK522_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNC41332.1"
                     /translation="MRYQTAATAIYLPVWRSRFLIIVLLLSLIALSGRAIYLQSLNKD
                     FLKQQGESRYSRVVELNAQRGKITDRNGEILAISAPVKSVWVDPQQLNVTPDQLIKLA
                     QILEVDVAEIHKRISDKDKRFVYLKRQLPPETAENIADLNIKGLYLKREFYRYYPTGE
                     MAAHVLGFTDIDGKGQEGVELGWEGILTGKHGSRRVIKDRIGRVVENVERIRSPQPGQ
                     NLVLSIDSKIQYLAHRELKQAVESHRAKAGSVIILDAQTGEVLALANYPTYNPNQRSS
                     IRKESLRNRAFVDTFEPGSTLKPFAVSIAMETGKIKPTSLLETAPGFLKVGKATIRDV
                     RNKGALSVSQVIQESSNVGTAKIALMLPPKTFWEMLSQSGFGTSTGSGFPGEVNGVLR
                     PYQKWKPIEQVTMSYGHGISVNLMQLARAYTIFASGGELKPISLLKRHSSVLGQRVLS
                     IETANAMSGMLEAAVQTKGTGLKAQVSGYRVAGKTGTAHKRLENKKGYAADRYISTFA
                     GYAPVSNPRLVVAVMIDEPSAGQYFGGAVAAPLFSEVMAAALWQLNVPRDAPGNIVSS
                     PLPVKFKEEG"
     gene            2595..4157
                     /locus_tag="PK522_06065"
     CDS             2595..4157
                     /locus_tag="PK522_06065"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNC41333.1"
                     /translation="MSSLISWKSDELLVDRILAELNVPIKRLVTDYRHIKAGNTFLAY
                     AGDRYDARSDIAKVIAAGANAVIWEKQQFSWNPEWVIPNCAVLDLKEKAGLISSRVYG
                     NPSRHLWLVGFTGTNGKTSCSHWYAEAMTILGKKTAVIGTLGNGFLQDLKYTANTTPD
                     AVLLQETLASCLKEGAQNLAIEVSSHGLVQGRVNGCEFAVAVLTNLSRDHLDYHKNMD
                     EYAAAKAKLFFWPNLKCAVLNLDDVFGIELVQQIGKRDLQIIGYGFNQSIDLKLNQGN
                     LRVLSGNNLQATIDGLEFDVEFEHEKAHIKTTLFSRFNANNLLAVIASLIASGVNLQD
                     AVAVLSRLKPVPGRMEKFGGGAQPTVIVDYAHTPDALEKVLTGLRETLVNSHLKQRPH
                     SRKARLICVFGCGGDRDRGKRPIAGGIVTRLADQVIVTSDNPRNEDARDIINEIVAGT
                     RYQNYIIEEDRASAIYQAIHNAHKNDIVLIAGKGAEAYQEIKGKRLPFDDREVTQQVL
                     HDLANMKMRVRR"
     gene            4154..5545
                     /gene="murF"
                     /locus_tag="PK522_06070"
     CDS             4154..5545
                     /gene="murF"
                     /locus_tag="PK522_06070"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNC41334.1"
                     /translation="MMTVQEAARMLNADWHGDDLVFGDVSTDSRQIKAGDLFVALSGD
                     RFDGHDYIGDTIERGAVAALVAREIKKTKLPQHFGWIHVANTRLALGQLASQWRDRFS
                     LPLVAVTGSNGKTTVKEMIASIFTKAASRQSNFTTDASIDQVLATIGNLNNDIGVPLT
                     LLRLRQHHVYAVVEMGMNHAGEIAYLTQLAKPDIAVITNAGAAHIEGLGSIELVAHAK
                     GEIFQGLDKPGVAIINADDKYAQLWYQLAGNRQIISFGMNAKAQIRADCHLLASSNKW
                     ILKFPKEESEIELQVLGQHNVMNALAAAAAALGAGVSEQVIIEGLSAFSGVKGRMQKK
                     SGLHQATLIDDTYNANPASVSAALSVLAHLSGRKIFVLGDMGELGVGGADLHRKIGEE
                     ASRAGIEKLLALGELSVNAAEGFGKGAMHFSNIDELLEEAEKLLDHNVTMLVKGSRFM
                     KMERVIQRFEVRH"
     gene            5603..6688
                     /gene="mraY"
                     /locus_tag="PK522_06075"
     CDS             5603..6688
                     /gene="mraY"
                     /locus_tag="PK522_06075"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNC41335.1"
                     /translation="MLLLIAQWIAQDVRVFNVFNYITLRTVLAVLTALVISFVIGPAM
                     IRKLTAYKIGQSIREDGPQSHLVKAGTPTMGGALILTSIVITILLWADLTNPYIWVVL
                     LTTLGFGVIGWVDDYRKVVHRNSKGLSAAAKFFWQSVIACCVAAYLYWIADTPEQTNM
                     IVPFFKEVAIPLGMVTFIVLTYLVIVGTSNAVNLTDGLDGLAIMPTVMISGALAIFAY
                     VTGHIVFAKYLGIPYIQNVGELAVFCGALAGAGLAFLWFNTYPAEVFMGDVGALSLGA
                     ALGVITVIVRQEIVLVIMGGVFVAEAMSVMLQVASFKLRGKRIFRMAPIHHHFELKGW
                     KENQVVVRFWIITLILVLLGLSTLKLR"
     gene            6685..8100
                     /gene="murD"
                     /locus_tag="PK522_06080"
     CDS             6685..8100
                     /gene="murD"
                     /locus_tag="PK522_06080"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNC41336.1"
                     /translation="MIFHEKKVLVLGSGETGISMVKWLSYLGAHLSLADSRANPPNPN
                     FLDDFIPHQKRFFGHFDLNLLENIDAIAISPGVPLAEAFVQEAIKRNIPVFGDIELFA
                     LTLNQLNQPKPIILAITGSNGKTTVTSMVGDMVKAAGWDVEVAGNIGPAVLKVLLQRL
                     STKGLPQCWVLELSSFQLETTQTLFANSAVVLNISEDHFDRYDGLDDYAAAKARIFIG
                     GTHSTQILNRDDPNVLAMALPDKKQVTFGLSKPSRDVDFGLLEFGSALWLVEGKRRLL
                     KADMLKIPGMHNVANALAALALCRSVGLPHEPLVDALLKFRGLPHRMQKIATIQGVDY
                     YDDSKSTNVGSAIAALNGMKGNIVLIAGGDGKGQDFSPLIEPIKQYVRALVLLGRDAE
                     KISQAIKGAAKPIHHVSTMEDAVRLSFLLAKPNDMVLLSPACASLDMFRNYIHRAEVF
                     TVAVKELESKGISLVESSPSL"
     gene            8097..9257
                     /gene="ftsW"
                     /locus_tag="PK522_06085"
     CDS             8097..9257
                     /gene="ftsW"
                     /locus_tag="PK522_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNC41337.1"
                     /translation="MIVQPTINRQKFAATSDYYLIGSVLALISLGLIMIYSASIAIAE
                     SEYGEAGSFHYLTRHASYIIIGLILGSIAFQIPTRVWRTLVPYLFLGGVALLILVLIP
                     GIGREVNGSQRWISLVIVNFQPSELMKLLMILYVSDYVVRKSLYLNDLRRGFLPLWLV
                     IGVVGVLLLREPDYGAFVVITSIAVVIMFLGGMSLKLFAGLASILVIGLVLLVLFEPY
                     RMDRIIAFMDPWADPFGKGYQLSHALIAFGRGEWFGVGLGGSVEKLFYLPEAHTDFLL
                     AVLAEELGFIGVMVVMMLFACLLIRAYQIGRLAASLENYFSALVAQGIGIWLGLQALI
                     NMGVNMGVLPTKGLTLPLMSFGGSSIVVSCIALAILLRIDSENRQRIRGMAV"
     gene            9292..10380
                     /gene="murG"
                     /locus_tag="PK522_06090"
     CDS             9292..10380
                     /gene="murG"
                     /locus_tag="PK522_06090"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNC41338.1"
                     /translation="MKHTLLIMAGGTGGHVFPGIAVADYMRSLGWRVVWLGTQNGMEA
                     VLVPKYGYPVEYINFSGIRRKRIMDWLLLPGKLLLAFWQSIKILIRNQPSIVLGMGGY
                     PAFPGGLMAVLLNKPLVIHEQNAIAGLTNKILARVADKILLGFPGTFPQKDNRVVVTG
                     NPVRSKISELPLPEQRFSDRTGPIKILVIGGSLGASVLNKVIPQALSRIPEAKRPIVQ
                     HQAGRQHIDELRENYREQGVEGQLIDFIDDMAASYAECDLVICRAGALTITELAAVGV
                     ASILIPFPYAVDDHQTANAEYLSKPGAAVLLPQHKLNADSLADKIMELTRAQLLDMAK
                     IARNLANFNSAQQVAQVCIAMEKANNET"
     gene            10370..11785
                     /gene="murC"
                     /locus_tag="PK522_06095"
     CDS             10370..11785
                     /gene="murC"
                     /locus_tag="PK522_06095"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNC41339.1"
                     /translation="MKHKVKSVHFVGIGGAGMGGIAEVLINLGYKISGSDLMENMTTE
                     RLQKLGALIYYGHDKENVANVDVVVTSTAVKADNPEIIAARERHIPVVPRAMMLAELL
                     RLRQGIAIAGTHGKTTTTSLVASILAEAGYDPTFVIGGKLEAAGSHAKLGRGEFIVVE
                     ADESDASFLYLQPILTVVTNIDADHMSTYGHDFARLKQTFVEFIEHLPFYGMAVLCID
                     DQNIREILPNITKPITTYGLSPDAQVSASSVQHDNGRMCFRVNIGVNGTARQLDITLN
                     LPGLHNVQNALAAITIANEIGVPDAAIVRALATFGGVERRFQRYGDIKIPGKGSFTLI
                     DDYGHHPAEIAATISATRGAFPGRRLILAFQPHRYTRTRDVFEDFVKVLSTVDILLLT
                     EVYAAGEEPIVAADSKSLTRAIRVLGKLEPIYVEMPDELPSVIHGIVEDRDVVLIMGA
                     GSIGKVAAWVSKAAKKLVAIG"
BASE COUNT         3309 a         2148 c         2942 g         3437 t
ORIGIN      
        1 cacgtttcag cttgccgaaa ttattacaga ggtcatgcgc acgggtgggt atcagaaatt
       61 atggcgaaga acggcatgct cggaagattg caagagcagt tgttatggca agagcgaaat
      121 acccgatatc taccacgttt cagcttgccg aaattattac agaggtcatg cgcacgggtg
      181 ggtatcagca tgggggtaag cagcatccgg cgacacggac ctttcaggca attaggattc
      241 atctcaacca agaacttgag gagctttcgt tagtattacc gcaatgtgtt gagctgctga
      301 atcccaaagg aaggttggta gtgattagtt ttcattcctt ggaagacaga attgtgaagc
      361 gttttatgcg agcaaaagca aatccagagg gtaatttgcc aagaaagatt ccgttgactt
      421 caaaggaagt tcaagaattg attcatcgag aggtgctgtt gataggcgat gccctaaagc
      481 ctacaagcat tgagattgca agaaatccgc gtgctagaag tgctgttatg cgtgttgcgg
      541 aaaaattaaa gaacgggagg tgaaatgatt aagctcaaca ttttgctggt attgatactc
      601 attttttcgg ggctaggtgt cgtgacagcg cagcatgaga cgagaaagct tgtcatttca
      661 cttgagaaag agcacaactt aactgatcag cttgcaatcg agcgtaatca attgttaatt
      721 gagcagcgca catgggcaat gcctggttat gttgaaaaaa ttgccaaaaa taaactcgat
      781 atgattgcgc cttcatcgag ccatattacg gttattactc ccgatcatgt tttaaattga
      841 gctaagccaa tcgatgggtg attagtgatg agatatcaaa cagcggctac agcaatttat
      901 ttaccagttt ggcgttcacg gtttctgatc attgtgcttt tattgagctt aatagcccta
      961 tcaggacgtg caatctattt acagagctta aataaagact ttttaaagca gcagggggag
     1021 tcgaggtaca gtcgagtcgt agaattaaac gcacagcgtg gaaaaattac tgatcgtaat
     1081 ggtgagattt tagcaattag tgcgcctgta aaatccgtat gggtcgatcc ccaacagctt
     1141 aatgtgactc ccgatcaatt aataaagtta gctcaaatat tagaagttga tgttgcagag
     1201 attcataaac gaatttcaga taaggataaa cgcttcgttt atttgaaacg ccaattaccc
     1261 cctgaaactg cagaaaatat cgcggactta aacattaaag gtctctatct caaaagagaa
     1321 ttttatcgat attatccgac aggtgagatg gcagctcatg tcctcggttt tacagatatc
     1381 gatggaaaag ggcaggaagg agttgagctg ggttgggaag gaattttgac agggaagcat
     1441 ggcagcaggc gcgtcattaa agatcgcatc ggtcgagttg tggaaaatgt tgaaagaatt
     1501 cgatcacctc agcctggtca aaatttagtc ttatcgattg acagtaaaat tcaatatctt
     1561 gcacatcgag aattaaaaca agcggtagaa tcccaccgtg ctaaagcagg gagtgtgatt
     1621 atactggatg cacaaaccgg cgaagtgtta gcgctcgcta attaccctac ctataatcct
     1681 aatcaacgtt cttccatccg caaggaaagc ttacgtaacc gtgcattcgt cgatacattc
     1741 gagccgggat caactttgaa gccttttgca gtatccattg ccatggagac cggcaaaatc
     1801 aaaccgactt cgctattaga aacagcacct ggttttctta aggtagggaa agcgacgatt
     1861 cgggatgtcc gaaataaagg ggcgttatcg gtatcacaag tgattcaaga gtcaagtaat
     1921 gtaggaactg caaaaattgc attgatgctt ccacccaaga cattttggga aatgcttagt
     1981 caatctggtt ttggtacttc aaccggatct ggatttccag gggaggtcaa tggggtattg
     2041 cggccatatc agaaatggaa gccgattgag caagttacaa tgtcctatgg tcacggtatt
     2101 tcggtcaatt taatgcagct tgctcgcgcc tatactattt ttgcatcagg aggtgaatta
     2161 aaaccgatat ctttgcttaa aaggcattct tcagtgcttg gtcagcgtgt tttgtccatt
     2221 gaaacggcaa atgcgatgag tggcatgcta gaggccgcag tacaaacaaa aggaaccggt
     2281 ttaaaagctc aggtgagcgg ttatcgagta gcaggtaaga cggggactgc gcataagcgt
     2341 ctcgaaaaca aaaaaggcta tgctgcagat cgatatattt cgacgtttgc aggctatgcg
     2401 ccggtatcta atccaagatt agtcgttgct gtcatgatcg atgaaccatc tgccggccaa
     2461 tattttggag gtgccgttgc tgcaccactc ttcagtgagg tgatggcagc cgctttatgg
     2521 caactcaatg ttcctcgtga tgcaccagga aatatagtga gttctccttt gccagtgaaa
     2581 tttaaggagg agggatgagt tctttaatct catggaaatc cgatgagctt cttgtagatc
     2641 gcattcttgc tgagctcaat gtgccaatta agcgactagt aaccgattat cgtcacatta
     2701 aagcaggaaa tacttttcta gcttatgcgg gggatcgtta tgatgcgcgt tctgatatag
     2761 ctaaagtgat tgctgcaggc gcaaatgctg taatttggga gaagcagcaa ttttcatgga
     2821 atccagagtg ggttatccca aattgtgcgg tattagatct caaagaaaag gcagggttga
     2881 tctcaagtcg agtgtatggg aacccttcgc gtcacctgtg gctcgttggt tttactggaa
     2941 ctaatggaaa aacatcgtgt agtcactggt atgctgaggc aatgacgata cttggaaaaa
     3001 aaacagcagt aattggtacg ttagggaatg gtttcctaca ggatctgaaa tatactgcta
     3061 atacaacgcc tgatgctgtt ttgctgcagg agacgctagc gagttgttta aaagaaggtg
     3121 cacaaaattt agcgatcgag gtatcttctc atggtctcgt tcaagggcgt gtcaatggtt
     3181 gcgagttcgc agtggctgtg ctgactaatt tatcgcgaga tcacttggat taccataaaa
     3241 atatggatga gtatgctgcg gcaaaagcca aactattttt ctggccaaat ctcaaatgtg
     3301 cagtattgaa cctcgatgat gtttttggta ttgaactcgt tcagcagatt ggaaaaaggg
     3361 atctgcaaat tatcggttat ggttttaacc aatcaatcga tttaaaacta aatcaaggaa
     3421 atctgcgagt acttagtggc aataatttac aagcgaccat tgatggtctg gaatttgatg
     3481 ttgaatttga gcacgagaaa gcgcatatca agactacatt attcagtcga tttaatgcca
     3541 ataatctatt agcagtcatt gcttccctca tagcgagcgg tgttaatttg caggatgcgg
     3601 ttgcagtcct gtcacgttta aagcccgtcc caggtagaat ggaaaaattt ggtgggggag
     3661 ctcagccaac ggtgattgtc gattatgccc atacaccgga tgcccttgaa aaagtgttaa
     3721 ctgggttgcg tgagacatta gtgaatagtc atttaaaaca aagacctcat tctagaaaag
     3781 ctagattgat ctgtgtattt ggatgcggtg gggatcgaga tcggggtaag agaccgattg
     3841 ctgggggtat cgtaacgcgt ttagcggatc aggttatcgt aaccagtgat aatccccgta
     3901 acgaggatgc gcgcgatatt atcaatgaga tcgtggccgg tactcgatac cagaattata
     3961 taatcgaaga agatcgtgct tctgcaattt atcaggcgat tcataatgca cataagaacg
     4021 atattgtgct aattgctgga aaaggcgctg aagcgtacca agagattaaa ggtaagcgat
     4081 tgccatttga cgaccgtgaa gttacccaac aagtacttca cgatttagcg aatatgaaaa
     4141 tgcgagtacg cagatgatga ctgttcaaga ggctgctcgc atgctgaatg ctgattggca
     4201 tggcgatgac ctcgtgtttg gtgatgtcag tactgacagc cgtcaaatta aagcaggtga
     4261 tttgtttgtg gcattatcgg gtgatcgatt tgatggacat gattatattg gtgacacaat
     4321 cgagcgaggt gccgttgcag ccctagttgc aagagagatt aaaaaaacca aattaccgca
     4381 acattttgga tggatacacg ttgctaatac tcgattagcg ctagggcaat tagcgtctca
     4441 atggcgtgat cggttctcac tcccattggt ggctgtgaca ggaagtaatg gaaaaacgac
     4501 agtcaaagag atgattgcgt ctatttttac taaggcggct tcccgtcaat ctaattttac
     4561 taccgatgct tcgattgatc aggtgttagc gacaatcggt aatcttaaca atgatattgg
     4621 cgtcccactt actttactaa gacttcgaca acatcacgtt tatgcagtag tagaaatggg
     4681 gatgaatcat gccggagaaa ttgcttattt aacgcagtta gcaaaacccg atattgcagt
     4741 gattaccaat gccggtgccg cccatatcga agggttgggt tcgattgagt tggttgcgca
     4801 tgcaaagggc gaaatttttc agggcttaga taagccaggc gtagccatca ttaatgcaga
     4861 tgacaaatat gcacagcttt ggtatcaact agcaggtaat cgccagataa taagttttgg
     4921 aatgaatgca aaagctcaga ttagagcgga ttgccacctg ctagcatcga gtaataagtg
     4981 gatcttaaaa tttccaaagg aagaaagtga gatcgaacta caagtactcg gacaacataa
     5041 tgtaatgaat gcattggctg cagctgctgc agcgttaggt gctggagtca gtgagcaagt
     5101 tattattgaa ggtttgtcgg catttagtgg cgtgaaaggt cgtatgcaga aaaaaagtgg
     5161 attgcatcaa gcaactttga ttgatgatac ttataatgca aatcccgcat ccgttagtgc
     5221 agcactttca gttttagcac atttatctgg aagaaaaata ttcgtattgg gtgatatggg
     5281 tgagcttggt gttgggggtg ctgatcttca tcggaaaatc ggtgaggaag caagccgagc
     5341 aggtattgaa aaattacttg cgctgggtga attgagtgtg aatgcggcag aagggtttgg
     5401 aaaaggagcc atgcattttt ccaatatcga tgagttatta gaagaagctg aaaaattatt
     5461 ggatcacaat gtcacaatgc tagttaaggg ttctcgcttt atgaaaatgg agcgagttat
     5521 tcagcgattt gaagtgcgac attgacttgc ttaacaaagg ttaaatacgc gtgatcaaaa
     5581 cggagatcac ataaggataa aaatgctgtt actcattgcc caatggattg cacaagatgt
     5641 tcgtgttttt aatgttttca actatataac attacggacg gtattggctg tattgacagc
     5701 attggtgata tcttttgtta ttggaccggc aatgatacgt aaattaacag cctacaagat
     5761 tgggcaatca attcgtgaag acgggcccca gtctcatttg gttaaagcag gcactccaac
     5821 aatgggcggt gctttgattt tgacgtcgat cgtcattacg atattactat gggcggattt
     5881 aacgaaccct tacatctggg tcgttttact gacgacattg ggattcggtg tcattggttg
     5941 ggtggatgat tatcggaagg tcgtgcatcg caattctaaa ggtctttcgg ccgcagctaa
     6001 atttttctgg caatccgtaa tcgcgtgctg cgttgcagcc tatctttatt ggattgcaga
     6061 cactcctgag caaaccaata tgatcgttcc cttttttaag gaggttgcga ttcctttagg
     6121 aatggtcact tttatcgtgc tcacttatct tgttattgtt ggaacgagta acgcagttaa
     6181 tttgacagat ggattggacg gcttggctat tatgccaact gtcatgatca gtggtgcatt
     6241 ggctatcttt gcttacgtaa cagggcatat cgtttttgcg aaatatttgg gtattcctta
     6301 tattcagaat gtgggcgagc ttgcagtttt ctgtggagca ttagctggtg ctggcctggc
     6361 ttttctttgg tttaacactt acccagctga agtctttatg ggcgatgtgg gagcgttatc
     6421 ccttggagcc gcgctaggtg tgattacagt tattgttcgc caagagattg ttttagtgat
     6481 tatgggcggg gtgtttgttg cagaagctat gtcagtcatg ctacaagtag cctcttttaa
     6541 gctccgaggt aaaagaattt ttcgtatggc cccgattcac catcattttg aattgaaagg
     6601 gtggaaggag aatcaggttg tcgttcgatt ttggattatt actttaattt tagttttatt
     6661 agggctctcg acgcttaaat tacgatgatt ttccatgaaa aaaaggttct agttctgggg
     6721 tcgggtgaga caggaatctc catggttaaa tggctttcat atttaggagc gcacttatcg
     6781 cttgccgata gtcgtgctaa cccacccaat ccaaatttct tggatgattt tatccctcac
     6841 cagaaaaggt tttttggtca ttttgacttg aacttactag aaaatattga tgcgatcgca
     6901 attagccctg gagttccgct agcagaagct tttgtgcaag aagccattaa gcgtaatatc
     6961 cctgtattcg gtgatattga gttatttgct ctaacactga accagctaaa tcaaccgaaa
     7021 ccgattatcc ttgccattac aggctccaat ggaaaaacga ctgtaacgag catggtgggt
     7081 gacatggtaa aagcggctgg atgggatgtg gaagtcgccg gaaacattgg gcctgctgta
     7141 ttaaaagtct tactgcagcg actttctacc aaaggactac cacaatgctg ggtacttgaa
     7201 ttatccagct tccaacttga aacgacccaa acactttttg ctaatagcgc agttgtactg
     7261 aatattagcg aagaccattt tgatcgctat gatggcctag atgactatgc tgctgcaaaa
     7321 gcaagaattt ttattggcgg cactcatagt acgcagattc ttaatcggga tgatccaaac
     7381 gtgcttgcaa tggcgttgcc tgataaaaag caagtgactt tcggtttgag taaaccaagc
     7441 cgagacgtcg attttggttt gctggagttt ggttctgctt tatggttagt tgagggaaaa
     7501 aggcggcttc taaaagcaga tatgctaaaa attcctggca tgcataatgt ggctaatgcg
     7561 ctggcagcat tggcactgtg tcgttcggta gggttaccgc atgaaccttt agtcgatgct
     7621 ttactgaaat ttagaggatt gcctcatcgg atgcagaaaa ttgcaaccat acaaggggtt
     7681 gattattacg atgattctaa aagtaccaat gtcggttctg ccatcgcagc attaaatggg
     7741 atgaaaggca atattgtgtt aattgcggga ggggatggga aagggcagga tttctcacct
     7801 ttaattgaac ccattaaaca atacgttcga gcgcttgtac tactggggag agacgcagaa
     7861 aaaataagcc aggctatcaa aggagctgct aaaccgattc atcatgtatc gacgatggaa
     7921 gatgcggtgc ggttgagctt tttattggcc aaaccgaacg atatggtttt gctttctccg
     7981 gcttgcgcaa gtcttgatat gtttagaaat tatattcatc gcgccgaggt ttttactgtg
     8041 gcggttaaag aattagagag taaaggtatt tcattagttg agagcagccc tagcctatga
     8101 ttgtccaacc aaccattaat cgacaaaaat ttgcagccac atcggattat tatctcattg
     8161 gatcagtatt ggcactgata agcctcggct taattatgat ttattctgca tccatcgcaa
     8221 ttgctgaatc agagtatggg gaagcaggtt cttttcatta cttaactcga catgccagct
     8281 atatcatcat cggcttgata ttaggttcta ttgcatttca aattcctact cgagtgtggc
     8341 gaacgcttgt tccctatttg tttttaggtg gagttgcctt actcatttta gtcctgattc
     8401 ctggaatcgg acgtgaagtt aatgggagtc aacggtggat atcgctcgtt atcgtcaatt
     8461 ttcagccatc agaactcatg aagctgttga tgattcttta tgtatcagat tatgtggtac
     8521 gcaaatccct atatcttaat gatcttcgaa gaggtttttt acctttatgg cttgttattg
     8581 gggtagtcgg tgtattgtta ctacgcgagc ctgattatgg tgcttttgtc gtcatcacat
     8641 cgattgcggt ggttatcatg ttccttggag gaatgagtct taagctattt gcgggactgg
     8701 caagtatttt ggtaatcggt ctggtgttat tagtgttatt tgaaccttat cggatggacc
     8761 gtatcattgc ctttatggat ccttgggctg atccattcgg taaaggttat caattaagcc
     8821 atgcattaat tgcttttggt cgaggagaat ggtttggcgt tggattgggt ggaagtgtag
     8881 agaaactttt ttacttacct gaagcgcata cggacttttt actcgcggta cttgccgaag
     8941 aattaggttt tatcggcgta atggttgtaa tgatgttatt tgcttgctta ttaataaggg
     9001 cctatcaaat tgggcgttta gcagcttcac tggaaaacta tttctcggct ttggtcgctc
     9061 aaggaatcgg aatctggcta ggactccagg cgctaatcaa tatgggggtt aatatggggg
     9121 tgttgccaac gaaaggatta acgttgccat tgatgagttt cggtggaagt agcatcgtag
     9181 tgagctgtat tgcattagcc attttgttgc gaatcgatag cgagaatcgc caaagaatta
     9241 ggggaatggc cgtatgactg atgcaaagcg tgttttttgt tagaggtcaa tgtgaaacat
     9301 acactgttga tcatggctgg gggtacggga gggcatgtgt ttcctgggat tgccgtggcc
     9361 gattatatgc gttcacttgg gtggcgggtt gtatggttag ggacacaaaa cggaatggaa
     9421 gcagtactcg tgcctaaata tggctatcct gtcgaatata ttaatttttc tggtattaga
     9481 agaaaaagaa taatggattg gttgctattg ccaggaaaat tgctactcgc tttctggcaa
     9541 agcatcaaaa ttttgatacg caatcaaccg agtattgtat tgggaatggg aggctatccc
     9601 gcattcccag gcggattaat ggcagttctc ttgaataaac ccctagtcat tcatgaacag
     9661 aatgcgattg ctgggttaac aaataaaatc cttgctcgag tggctgataa aatcttatta
     9721 ggttttcccg gtacatttcc tcaaaaagat aatcgtgttg ttgtcacggg aaatccggtg
     9781 cgaagtaaaa tttcagaatt acctttgccc gagcaacgat tttcagatcg aactggacct
     9841 attaagattt tggtcattgg gggtagcttg ggagcgagtg tattgaataa agtaataccc
     9901 caggcgctta gtcgtatacc tgaagcaaaa cgtcccatcg tgcaacatca agcaggtagg
     9961 cagcatattg atgaattgag agagaattat cgtgagcaag gtgttgaagg gcagttaatc
    10021 gattttattg atgatatggc agccagttat gccgagtgtg atttagttat ttgtagagcc
    10081 ggtgcgctaa cgattactga attagcagca gttggtgttg caagcatttt aatacctttt
    10141 ccttatgcag ttgatgatca tcaaacggca aatgctgaat atctttccaa acctggtgcg
    10201 gcagtgttgc taccacagca taaattgaac gctgatagtt tagccgacaa aataatggaa
    10261 ttgactcgag cgcagttgct tgatatggca aaaattgcac gaaatttggc aaatttcaat
    10321 tctgcgcaac aagtcgcgca agtatgtatc gcaatggaga aagcgaacaa tgaaacataa
    10381 ggttaaatct gttcattttg ttggaattgg tggtgccggt atgggcggta ttgctgaggt
    10441 gttaattaac ttaggttaca agataagcgg ctctgaccta atggaaaaca tgacgactga
    10501 acgcttacag aaattaggcg ctttgattta ctatgggcat gataaagaaa atgtggcaaa
    10561 tgttgatgtg gtcgtaacct caacggctgt taaagcagat aaccctgaaa ttatcgcagc
    10621 gcgtgagcgc catataccgg ttgtaccgag agcgatgatg cttgcagaat tgctgcgttt
    10681 gcgacaagga atagccattg caggaacaca tggaaaaaca acgacgacga gtttggttgc
    10741 gagtattctg gcagaagctg gatatgaccc tacctttgtt ataggcggaa agcttgaagc
    10801 tgcaggcagt catgctaagc tgggcagagg tgaattcatt gtcgtggaag cagatgaatc
    10861 tgatgcttca tttttatatt tgcaacccat cttaacagtt gtaacgaata tcgatgcaga
    10921 tcatatgtcc acgtatggcc atgattttgc aaggctcaag caaacgtttg tggaatttat
    10981 tgaacatctc cctttttacg gtatggctgt actatgtatt gacgatcaaa acattcgtga
    11041 aattcttccc aatataacga aaccaattac aacctatggt ttatcaccag atgcacaagt
    11101 cagcgcatct tcagttcaac acgacaatgg cagaatgtgt tttcgagtaa atattggtgt
    11161 gaatggaacc gcgcgccagc ttgatattac gttaaattta ccagggttac ataatgtgca
    11221 gaatgcgctt gctgcaatta ccattgctaa tgaaatcggt gtgcctgatg ctgctatcgt
    11281 tcgagcgctt gcaacttttg gaggagttga gcggcgattc caacgttatg gcgatattaa
    11341 aatccccggt aaaggaagtt ttaccttaat tgacgactat ggccatcatc ctgctgaaat
    11401 cgctgcgaca atctcagcga cccgaggcgc ttttccaggg aggaggctca tattggcatt
    11461 tcaaccccat cgctatacac gtacacgtga tgtgtttgag gattttgtta aggtattatc
    11521 cacagtagat attttgttgc tcacagaagt atatgccgca ggggaagagc cgatcgttgc
    11581 ggcagacagt aaatcattaa ctcgggcgat tcgtgtttta ggaaaattag aacccatcta
    11641 tgtggagatg ccagatgagt tacccagtgt aattcatggg attgttgaag atagagatgt
    11701 tgtgcttatt atgggagcag gctcaattgg aaaggttgcc gcatgggtgt caaaagctgc
    11761 aaaaaagttg gttgcgattg gataatcgag gaataaaaac tttccgtgta ctttggtaag
    11821 aggttttaat gcaatc
//
LOCUS       DAOQLJ010000066        11581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2155_length_11581_cov_5.794725, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000066 DAOQLJ010000000
VERSION     DAOQLJ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 11581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11581
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2155_length_11581_cov_5.794725"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..205
                     /locus_tag="PK522_06100"
     CDS             56..205
                     /locus_tag="PK522_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41340.1"
                     /translation="MCSGDIVHWFRMDGIVTELYDVITLPNVNQPMALGFKTDEIRRM
                     ISIEQ"
     gene            328..1677
                     /gene="chrA"
                     /locus_tag="PK522_06105"
     CDS             328..1677
                     /gene="chrA"
                     /locus_tag="PK522_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNC41341.1"
                     /translation="MVESQEPRMPRSIDFWDAFRFWLKLGFISFGGPAGQIAIMHREL
                     VENRRWISEQRFLHALNYCMLLPGPEAQQLSVYIGWLLHRTWGGIVAGLLFILPSLFI
                     LIVLTWIYLAFQDAPVVLGIFYGIKPAVTAIVIHAAYRIGSRTLKNGLLWAIACAAFI
                     AIFALKIPFPIIVAIAALIGYLGGRFSSDYFKLGSHPTKQMNQSFGPAVIDDDSPRLS
                     HTFFRWAHLAKIVVIGMLLWLIPMSLMMQYLGWQHALTQMGWFFTKAALLTFGGAYAV
                     LPYVYQGAVDYYQWLLPYQMIDGLALGETTPGPLIMVVTFVGFVAGWNLTLMGPDHLL
                     LAGIIAGVVVTYFTFLPSFIFVLAGGPLVESTHGNLKFTAPLTAITAAVVGVIINLAL
                     FFAWHVLFPGNQQNFDFVAGLITLFAVIALFRFQVGVIPVLIYSGILGLSLTVLGLL"
     gene            1873..2367
                     /locus_tag="PK522_06110"
     CDS             1873..2367
                     /locus_tag="PK522_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNC41342.1"
                     /translation="MRLPKILISFFLLSLYGISLLANAEQAQLKHFASGSYQQLLKQY
                     AGKPIVVMIWSIHCTICLEKMPLLSELRKNKPNINLIMIATDDVSATNQVRSILNENK
                     LDQVDNWIFAYANPQKLRYEIDPKWYGEVPRTYFLDKDHQRVGISGSVPRETMETMIR
                     EIFN"
     gene            2528..3736
                     /locus_tag="PK522_06115"
     CDS             2528..3736
                     /locus_tag="PK522_06115"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNC41343.1"
                     /translation="MKRYVFIGICLLLLIFSGYLIAESETNLPEQKIYTAAAFGPDGR
                     LWRIVPSQNFVSVDYSTDYGKSFSKQVRVNSVDIPIHLWDENPPTLSVDRKGRIYALY
                     FADDEKASTSFFSYSDNGTQFSVPIKISTEANHWYHYQTEMLIDVTNRVHFIWHDVRD
                     RAEYKKQGGGDLSIYHTATKVGEIIQFPAFDRRIAKNICSCCRTAMAEDIDGSLVILA
                     RFVYPGNIRDHGLLKLSADGEVSEPWRVTFDDWQIEGCPAHGPALSISADGRYHITWF
                     TQGEKRSGLFYAWSDDQGKTFSDPIQIGDSNRLPGRGDVLALGRQVALVWKIFDGDKT
                     YIEAMYSEDRGLHWSTPRSIAESSSQSTHPALISDGKQIFLSWNSMDTGYRLIPIKQS
                     DSNDQLSIKP"
     gene            complement(3749..4183)
                     /locus_tag="PK522_06120"
     CDS             complement(3749..4183)
                     /locus_tag="PK522_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41344.1"
                     /translation="MKQSSLKKAAIFISIFILIGQVNIASSHQLNNSLTAADAVDHFL
                     VTCGERGNHHLFVQIDDLSVVDSRQFNVLVAKDKVISSATNEDGKSSAVLRVAGGTGT
                     YHVYVSQTRGGEKAANYQLLYHCEDVDNDHLETSIFTKINQP"
     gene            4614..4850
                     /locus_tag="PK522_06125"
     CDS             4614..4850
                     /locus_tag="PK522_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNC41345.1"
                     /translation="MVAKAIFYHAGCPVCVAAEHAVANAIDRSKYQVEIVHLGTDKSR
                     VAEAEAAGVKSVPALVMDGAAFHINFGAGIEVLK"
     gene            complement(5018..5956)
                     /gene="lipA"
                     /locus_tag="PK522_06130"
     CDS             complement(5018..5956)
                     /gene="lipA"
                     /locus_tag="PK522_06130"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNC41346.1"
                     /translation="MTIRETGSAKTARNPIKINTQQSSELLRKPSWIRVRSSNSQTFN
                     EVKRMLREHKLHTVCEEASCPNIGECFGKGTATFMILGDLCTRRCPFCDVAHGRPLAP
                     DPEEPVHLAQSIAALKLKYVVITSVDRDDLRDGGAQHFADCIQQIRLHSPTTKIEILV
                     PDFRGRLSVALEKLATHLPDVLNHNLETVPRLYKQCRPGADYLHSLQLLSDFKLAFPN
                     IPTKSGLMVGLGETDDEIKQVMRDLREHHVDMLTIGQYLQPSIGHLPVMRYVAPEDFK
                     VFEHLAESMGFSHAACGPMVRSSYHADQQAHEAGIS"
     gene            complement(5973..6560)
                     /gene="lipB"
                     /locus_tag="PK522_06135"
     CDS             complement(5973..6560)
                     /gene="lipB"
                     /locus_tag="PK522_06135"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNC41347.1"
                     /translation="MGMKDYGETWQAMKTFTMNRDTDTSDEIWLLQHFPVYTQGIAGK
                     PEHLLYNNQIPVVNTDRGGQITYHGPGQLIVYTLLEFRRLGLTVRGLVTQLEKAVIDL
                     LAVYCIKAESRREAPGVYVNGAKIASLGLKIKNGFCYHGIALNVDMDMEPFAAINPCG
                     YKGLQMTQLVDLGVPDDIDVISQRIAEKLIIRLSE"
     gene            complement(6625..6894)
                     /locus_tag="PK522_06140"
     CDS             complement(6625..6894)
                     /locus_tag="PK522_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HNC41348.1"
                     /translation="MDAEESSLIEYPCDFPIKVMGKSQSGFAQVVLSIVKTHAPDFDD
                     AMMEVRLSKNGTYLSLTCTILATSRTQLDNLYQELHDHPMVMLVF"
     gene            complement(6920..7786)
                     /locus_tag="PK522_06145"
     CDS             complement(6920..7786)
                     /locus_tag="PK522_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HNC41349.1"
                     /translation="MIYLNGKFLPIEQAYIPVLDRGFIFGDGVYEVIPVYSRKAFRLE
                     HHLARLQDSLNGIRLSNPHTNDQWCQLITQLIDYHDSDDQYIYLHITRGVAKRDHAFP
                     VNISPTIFMMSNALLTPPKELLQKGVLAITAEDNRWGRCDIKAISLLPNVLLRQLAVD
                     AGATETILLRDGFLTEGAASNIFIVKESKLLAPPKSHLMLPGITYDVVLELVENHALP
                     YDVRPVAEQELRSANEILLTSSTKEIMPIVSLDGKLIGDGRPGPFFHKLNQLYQHYKE
                     LTMRGKQVPRDY"
     gene            complement(7793..8911)
                     /locus_tag="PK522_06150"
     CDS             complement(7793..8911)
                     /locus_tag="PK522_06150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNC41350.1"
                     /translation="MKLWVMFMVSLIVTPSFAQLPELSIAAKSYTLLDYQSGQTLLSQ
                     NAHERYEPASLTKLMTAYVVFSALKEKRISMDQVVPVSTTAWKMVGSRMFIEPNKPVT
                     VKELINGMIVQSGNDACVALAELIAGSEDIFAQLMNKEAERLKMTNTHFMNSTGLSHP
                     NHYSSAHDLSILASAIVRDFPEYYSLYSMRDFTYNGITQSNRNRLLWLDPNVDGMKTG
                     WTDKAGYCLITSAKRDNRRLISVLMGAASESMRSKESQRLLNFGFQFFDTAQLYKKHQ
                     HISSIQVWKGSQNKLNVGFDHDVYFSLPKGQSEKLKAKMEYKQPLIAPINKGQEVGMV
                     RFSIDDQQIAAYPLVALEAVDTAGIFGRAWDSVQMLFN"
     gene            9176..10027
                     /locus_tag="PK522_06155"
     CDS             9176..10027
                     /locus_tag="PK522_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNC41351.1"
                     /translation="MKHCDIDYKKIATAQPGIITKIIEINEESQNCFREIDALLRSDQ
                     AIAAFILRVANSPIYSRNQVIKTLPIAISLLGVSIIRSLVILAFSRAVFAKSRNELVC
                     KHVWHHSLLTAIASHQICYKLGNHEAGEEAFVAGLLHDIGKVLLFTHVQNEYMEALHY
                     ALEQHCPCADAEQKFLGINHRQVGEEAIKAWKLPEHFNRFVHNDLDRLAVGHIQDKVL
                     LSLAAANSIIKGRGFGANTIDPSIQKEKLIQYGLSEEFSSQLLDEAFFEELLTNDIYQ
                     FCANVHN"
     gene            10014..>11581
                     /locus_tag="PK522_06160"
     CDS             10014..>11581
                     /locus_tag="PK522_06160"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNC41352.1"
                     /translation="MSIINLSHEIKTILPSSLELGKDTIQITSLEQHVWSDIENKILS
                     RLNHLKCPQLIDSTSLLLHKLISNMVNTMHHRVFQQFVESDLYLGGVSGDNFFEELYQ
                     NEIEEHGDKNIASYCRRNDTQITLGFPDDLETLLTIRYPICCDSAVYLDSKLSEALGF
                     SLIKQYAGEASAHYYLAKLTRVNFEYQSSHFPGYFPLLLTRASELDKMKAIFSKLSYG
                     IVCFSSTGEILSISSAILDSLKLDSSSLSIQTFSKIIPPHFYNDVIWGLALETTEGIF
                     ENYRVRIKLPNDSGQSILFNISGYRSHDSTIQSLWQVVFLDPKSVDSLTEGSILNEAR
                     IHNITRNYVPQLVEQKARDVVRLGGKELTNEECYITVLFCDIVNFTAYVENNEKEESV
                     IHTLNLVLGRISRTVNQFEGSIDKFMGDCVMVLFKNPLNAVLAALEIQKHSLDINSLR
                     KNAGKEMLLLRIGIHWGQVIIGNIGISGRLDWTTIGDVVNTASRLEKNCQPGAVLISQ
                     ILYEVIATVEQPGI"
BASE COUNT         3399 a         2208 c         2337 g         3637 t
ORIGIN      
        1 gaaacgaaag ttaatcgatg acacacaatg aaactcagcg taaaaatggt tttaattgtg
       61 tagtggcgat attgtgcact ggttccgcat ggatggaatt gtaaccgaat tatatgatgt
      121 gattacctta cccaatgtga accagccgat ggcgctaggt tttaagactg atgaaattcg
      181 gcgcatgatc agtatcgaac aataagaagt atggatggct tgtcatacta atcccgatca
      241 atttctcaaa aacccccaag taatatcatt atcgatatag actaatgcct ttttgaagtt
      301 aaatggcaaa tggggaaggg tatcgatatg gttgaatcac aagaaccgag aatgcctcgg
      361 tcgattgatt tctgggatgc ctttcggttt tggttaaaac taggatttat cagctttggt
      421 ggccctgcgg ggcaaattgc cataatgcat cgcgagcttg tcgaaaatcg tcgatggatt
      481 tctgaacagc gcttcttgca tgcattaaat tattgcatgc tattacctgg acctgaagca
      541 cagcaacttt cagtctatat cggctggttg ttgcaccgta catggggtgg aattgttgca
      601 ggattgctct ttattctgcc ctccttgttc attttaattg tcctcacgtg gatttatctt
      661 gcattccagg atgctcccgt tgttttaggt attttttatg ggataaagcc ggctgttacc
      721 gcgatcgtta tccatgcagc gtaccggatt ggatcgcgta cgctcaaaaa tgggctgctt
      781 tgggcaattg catgcgcagc tttcattgct atctttgcac ttaaaatacc atttccaatc
      841 atcgtagcca tagcagcgtt gattggatat ttgggagggc gtttcagttc tgattacttt
      901 aagttaggta gtcatcctac aaagcaaatg aatcaatctt ttggaccagc agttatcgat
      961 gatgattcgc cacggttatc gcataccttt tttcgatggg cgcatctggc taagattgtt
     1021 gtaattggca tgttactttg gctaattccg atgagtttga tgatgcaata tttaggttgg
     1081 caacatgcct tgactcagat gggttggttt tttaccaaag cagctttact aacatttggt
     1141 ggcgcgtatg cggtgttgcc atatgtttac caaggcgcag ttgattatta tcaatggcta
     1201 ctaccctatc aaatgattga tggtcttgcg ttaggagaga ccactcccgg accacttatc
     1261 atggtagtta cttttgtggg gtttgttgca ggatggaatc tcacattaat ggggcctgat
     1321 catttgctgc tagcaggtat cattgcgggg gttgttgtta cgtattttac cttcttaccg
     1381 agttttattt ttgtactagc gggtggaccg ttagttgagt caacacatgg aaatttgaaa
     1441 tttacggcgc cactgacagc gattacagca gctgtagtcg gcgttattat taacttggcg
     1501 ttattttttg cttggcatgt attatttcca ggtaatcagc aaaattttga ttttgtggct
     1561 gggttaataa cattattcgc ggttatagca ttattccgtt tccaggtcgg ggtcatccca
     1621 gttctgattt attcagggat actaggatta tcgcttaccg ttctaggcct tttataaaaa
     1681 gcttggcatg aagtactaag ctaaattcac catgggtaaa tcaatcgttt aattgatccc
     1741 acctgcgaca aattgtcaca tgttcaagaa actcagtttt atggataatt gaaatcatca
     1801 attaaattat tttttgagtg tcgtggtgta gttatttaat catcccacga tcgaacatta
     1861 gcgaatgcca tcatgagatt acctaaaatt cttatttctt tttttctact gtcactctat
     1921 ggaataagct tattggcgaa tgctgagcag gcgcagctga aacattttgc ttctggtagt
     1981 taccagcaat tgctgaagca atatgctggc aaaccaatcg tagtgatgat ttggtctatt
     2041 cattgcacga tctgtttaga gaaaatgcca ttgttgagtg agttgcgtaa aaataaacca
     2101 aacattaatt tgatcatgat tgccactgat gatgtttcag cgacaaatca ggttaggtcc
     2161 atattgaatg aaaataaact cgatcaggta gataactgga tttttgcgta tgcgaatcca
     2221 caaaagcttc gctatgagat tgatccgaaa tggtatggtg aagtgccccg cacttatttt
     2281 ctcgataaag atcatcagcg tgtcggtatt agtggttctg taccgcgtga gacaatggaa
     2341 actatgattc gggaaatttt caactaaatt tctgcaatag taatttaaca cgcttggtca
     2401 tgctgattaa tagcacatgt ggaaattact ggggtttctc tgattctggt tattccgctt
     2461 tacatatttg atcctgaacg aattgagatt cttaatttta gaaaaatctt ttgcacaaaa
     2521 gagcgccttg aaacgctatg tttttattgg gatatgttta ttattgctga ttttttcagg
     2581 gtatttaatc gccgaatctg aaaccaatct accagaacaa aaaatctata cagcagcagc
     2641 atttggccca gatggccgat tatggcgaat tgtcccttct cagaatttcg tttcagttga
     2701 ttattctact gattatggaa aatcttttag caaacaggtt agggttaatt cagttgatat
     2761 acccatacat ttatgggatg agaatcctcc gacattatcc gtagaccgaa aaggtagaat
     2821 ttatgcgctt tactttgcag atgatgagaa agcaagcact agcttcttta gttattctga
     2881 taatggaact caattcagtg tgccgattaa aataagtacg gaagccaatc attggtatca
     2941 ctaccaaacc gaaatgctca tcgatgtaac caatcgtgtg catttcattt ggcatgacgt
     3001 gcgcgatcgt gcggaatata agaaacaggg tggtggagat ttatcaattt atcatacggc
     3061 gaccaaggtc ggggaaatca ttcaatttcc agcctttgat cggcgtattg cgaaaaatat
     3121 ctgttcttgc tgccgtaccg caatggcgga agatatcgat ggcagcttag tcatattggc
     3181 gcgttttgtt tatccaggaa atattcgcga tcatggtttg ttaaaattat cagccgatgg
     3241 agaagtgagc gagccgtggc gggttacgtt tgatgattgg caaatcgaag gatgtcctgc
     3301 ccatggtcct gctttatcga tcagtgccga tggcagatac cacatcacct ggttcaccca
     3361 aggggagaaa cgcagtgggc ttttttatgc gtggtctgat gatcaaggga aaactttctc
     3421 agatccaata caaatcggtg attccaatcg attacctggg cgaggggatg tattagcctt
     3481 aggtagacaa gtagctttag tatggaaaat atttgatggc gataaaactt atatagaagc
     3541 gatgtattct gaagatagag gattacactg gtcaacgccc cgatcaatag ctgaatcgag
     3601 ttctcaatca acacatcctg ccttaatttc tgatggaaag cagatttttc tatcttggaa
     3661 tagtatggat actggctatc gcttaattcc gattaagcag tctgatagta atgaccaact
     3721 atcaataaaa ccctaaatcc tgattctgct atggttgatt aatcttagtg aagatcgatg
     3781 tttcaaggtg atcgttatcg acatcttcgc agtgataaag taattgataa ttagctgctt
     3841 tctcgccccc tcgagtctga ctgacatata catgataagt cccagttccg cctgctaccc
     3901 gaagaacagc gcttgatttc ccgtcttcat tcgttgcgct tgaaatgact ttgtcttttg
     3961 caacaagaac attgaattgc ctgctatcaa ctacgctgag atcatcgatt tgtacaaata
     4021 agtgatgatt acccctttct ccacaagtga ccagaaaatg atcgacagca tcggctgcag
     4081 taaggctatt attcaattga tgcgatgaag caatgtttac ttgtccaatt aatatgaaga
     4141 tactaataaa aatggcagct ttttttaacg atgattgttt catgataatt accttaaata
     4201 cacttattat cgatggagct agaatttttc ctggaaatta ggattaacta ccataaagcg
     4261 cctgaattca ataaaagtgt atagattgat agtatgtcgc aagtgactta gaaaattaac
     4321 gcttgctcag actaaatctt atggactgga gagtcaatgc gacagtttgt cacactgatg
     4381 tgatggactg acccgatttt tccagaggtt tcttaatttg agttaaaaga ccacattagc
     4441 tgaccccttc agttggcaat attgatatct cgctgtgtcg attacaatcg cgtattgccc
     4501 aaaccaaagc tgattcaccg atacctttag actcatttcg tattggaaga gactctatgt
     4561 tataatctat gccaatatat tgataatctt tattttgaag aggaaaaaac tttatggtag
     4621 ctaaagcaat tttttatcac gcagggtgtc ccgtttgtgt tgcagctgag catgcagtag
     4681 cgaatgcgat tgatcgttca aaatatcaag ttgaaattgt tcacctgggt acagacaaat
     4741 ctcgtgttgc tgaagcagaa gctgccggcg ttaaatcagt acctgcgctc gttatggacg
     4801 gggctgcatt ccatattaac tttggcgcag gaattgaagt tttgaaataa ataagtttag
     4861 caattaaact ttttgatatt caaaaaaccc cgatatgggg ttttttgttt ttcaacgatt
     4921 aattttagct gtttctttta aattaaatcg taaagttgat tgatatcaac gctactagga
     4981 tcagatcact atctacttat ctgttatctg attcaattta actgatccct gcttcatggg
     5041 cctgctgatc tgcgtgataa cttgatcgta ccatagggcc acaagctgcg tgactgaacc
     5101 ccattgattc tgctagatgc tcaaatactt taaaatcttc tggagccaca tagcgcataa
     5161 ctggtaaatg acctatgctt ggttgcagat attggcctat cgttagcata tcaacgtgat
     5221 gttctcgtaa atcacgcatg acttgtttta tttcatcatc cgtttcgcct aaaccaacca
     5281 taagtcctga cttggttggt atgttaggaa aagcaagttt gaaatcactc agtaactgta
     5341 gtgaatggag ataatcagca cctgggcgac attgtttgta aaggcggggt actgtttcta
     5401 agttgtgatt caaaacatca ggaagatgtg tggcaagctt ttctagcgct accgataatc
     5461 gacccctaaa atcaggaacc aagatttcaa tttttgttgt aggggaatgt aaccgtattt
     5521 gttgaatgca atcagcaaaa tgttgagcac caccatctct caaatcatcc cgatctacac
     5581 tggtgatgac tacatatttc agtttcagcg cagcaatgga ttgagcgagg tggaccggct
     5641 cctcaggatc gggcgccaaa ggtcttccat gcgctacatc acagaatgga caacggcgcg
     5701 tgcacagatc acccaaaatc ataaaagtgg ccgttccttt accaaagcac tcgccaatgt
     5761 ttgggcatga tgcttcctca caaactgtat gtagtttgtg ttctcgcaac attcgtttca
     5821 cttcattaaa tgtttgacta ttcgaagagc gtacccgaat ccacgacggt ttacgtaata
     5881 gttcgctgga ttgttgtgta ttgattttaa tggggttccg tgccgttttg gcgcttcctg
     5941 tttctcttat agtcatggtt tataattgtt ccttattcgg atagtcttat gattagtttt
     6001 tcagcaattc tctgacttat aacatcaata tcatcaggaa cacctaagtc gactaattgt
     6061 gtcatttgta gtcctttata tccacagggg ttaatggcag caaaaggttc catatccata
     6121 tcgacgttta aagctatgcc atggtagcaa aagccattct taatttttaa tcctagcgat
     6181 gcaatttttg caccattaac atagacaccc ggcgcttcac gcctactctc ggctttgatg
     6241 caatacactg ctagtaaatc gataacagcc ttctcaagct gggtaactaa ccctcttaca
     6301 gttaaaccga ggcgacgaaa ctcaagtagt gtatagacaa tgagttgtcc cggtccatgg
     6361 taagtaatct gtccccctcg atcggtattc actacaggaa tttggttatt gtagagcaaa
     6421 tgttctggtt tccctgcaat tccttgcgta taaactggaa aatgttgaag tagccagatc
     6481 tcatcggatg tatcggtatc acgattcatt gtgaaagttt tcatggcttg ccatgtttca
     6541 ccataatcct tcattcccat atattttatt tgagtaggaa tgaatgtttc tgatcgcgac
     6601 ttagtaatcg ttctcacgag attattaaaa taccagcatt accatgggat gatcatggag
     6661 ctcttggtag agattatcta attgagtgcg tgaagtcgct aaaatggtac aggttaagct
     6721 taagtaagtg ccgtttttac ttaaacgaac ttccatcatt gcatcatcga aatctggcgc
     6781 atgggtttta acaatactca ataccacttg agcaaagcca ctttgtgatt ttcccattac
     6841 tttaattgga aaatcacaag gatattcaat gagcgaagac tcttctgcat ccatagtact
     6901 atcgtattgt atttaaattt caatagtctc taggaacttg cttacctcgc atggtcagtt
     6961 ctttatagtg ttggtagagt tgattaagtt tatgaaagaa ggggcctggt ctaccgtcac
     7021 cgatcaattt accgtctaat gaaacaatcg gcataatttc tttcgtagaa gaagttagta
     7081 gaatttcatt ggccgatcgt aactcttgct cagcgactgg ccttacgtca taaggtaaag
     7141 catggttttc taccagttca agcacaacat cgtaggtaat gcctggtagc attaggtggc
     7201 tcttgggagg tgctaacagc ttgctttctt taacgataaa gatattgctc gctgcacctt
     7261 cagttaagaa tccatctcta agtaatatgg tttcagtcgc tcccgcatca actgccagtt
     7321 gacgcagtaa cacattgggt agcaatgaga tagcttttat atcgcatcgg ccccaacgat
     7381 tatcttcagc ggtaatggcc aaaacacctt tctggagtaa ttctttaggg ggtgttagta
     7441 gcgcattgct catcataaat atcgtaggtg agatattgac gggaaacgca tgatctcgtt
     7501 ttgctacacc ccgggtaata tgcaaataaa tatattgatc atcgctgtca tgataatcaa
     7561 tcagttgtgt aattaactgg caccattgat cattcgtatg gggattgctc agtcgaattc
     7621 catttaagct atcttgtaaa cgcgctaagt ggtgctccaa tcgaaaggct ttacgagaat
     7681 aaactggtat aacttcataa acaccatcac caaagataaa tcctctatcc aatacaggaa
     7741 tataggcttg ttcaattggc aaaaattttc cgttcagata aatcatcatt aattaattaa
     7801 ataacatttg aacactatcc caagcgcggc caaaaatacc tgcggtatct actgcctcta
     7861 aagctactaa tggataagct gcaatttgtt gatcatcgat actaaatcta accataccaa
     7921 cttcttgacc tttattgata ggcgcaatca gcggttgctt atattccatt ttagctttta
     7981 atttctcaga ttgaccttta gggagtgaga aataaacatc atgatcaaag cccacattta
     8041 atttattttg tgaacctttc catacctgaa tggatgaaat gtgttgatgt ttcttataaa
     8101 gttgtgccgt atcaaaaaat tgaaagccaa aattcaatag gcgctgactt tctttgctcc
     8161 tcatactttc tgatgcagca cccattaata cagaaatcag gcgacgatta tcgcgttttg
     8221 ctgaagtaat tagacaataa cccgctttat cagtccaccc cgttttcata ccatctacat
     8281 tgggatccaa ccacaataat cgattccggt tggattgcgt gattccattg taggtgaaat
     8341 cacgcatcga atagagcgaa tagtattctg ggaaatcacg aacaattgca gaagctagaa
     8401 tcgataaatc gtgtgcacta ctatagtgat taggatgcga taggccggtc gagttcataa
     8461 aatgagtatt tgtcattttg agacgctcag cttctttatt cattaattgc gcaaagatat
     8521 cttctgaacc tgcaatcaat tctgcgagag caacacaggc atcgttacct gattgtacga
     8581 tcatcccatt aattagctct ttgacagtaa caggtttatt aggctcaata aacattcttg
     8641 agccaaccat tttccatgcg gttgttgaaa ctgggacaac ctgatccatc gaaattcgtt
     8701 tttctttaag cgcagagaac accacatacg ctgtcatcaa cttagtcagt gacgctggtt
     8761 catagcgttc atgtgcattc tgactaagaa gcgtttgacc actttgataa tctaaaagtg
     8821 tgtaggattt tgctgcaatc gataattcag gtagttgcgc aaatgaaggt gtaacaatca
     8881 agctcaccat gaacataacc caaagtttca ttattttaag aataaaattc atttttcttg
     8941 tccgctcaat ttcaaaaaaa caccctaaac actattaata ctcctgatcc cagatcaata
     9001 gttgcgatat acccattatt atctcaacgt tgcttgataa aaaattactt gacaaatccc
     9061 atttaaattt aaccgtgcaa atgccgtagt ttcacttaca cattcgataa gtaacctata
     9121 aggtcaatat gatagcaaat taagacatcg ttaatttgtt gttattaaac attctatgaa
     9181 acactgtgat attgattata agaagattgc tacagctcaa ccgggaatta tcacaaagat
     9241 cattgaaatc aatgaggaat cacagaattg ctttagagag atagatgctc tattacgttc
     9301 tgaccaagcg attgccgcat ttatccttcg agtagcgaat tcacctattt atagtcgaaa
     9361 tcaagttatc aaaacactac cgattgccat tagtttattg ggtgtaagta ttattcgatc
     9421 tctagtcatt ttagcgtttt cgagagctgt ttttgctaaa agtagaaatg aacttgtttg
     9481 taagcatgtt tggcaccatt cactattaac cgcaattgca agccatcaaa tttgttataa
     9541 gctcggtaat cacgaagcgg gggaagaagc attcgttgct ggactactcc atgatatagg
     9601 gaaagtgctt ctttttaccc atgtgcagaa tgaatacatg gaggcactgc attatgcgct
     9661 tgagcagcat tgtccttgtg ctgatgcaga gcaaaaattt ttaggaatta accaccgtca
     9721 agttggtgaa gaagcaataa aagcatggaa gctaccggaa cattttaatc gttttgttca
     9781 taacgatcta gataggttag cagtaggcca tattcaagat aaagtacttc tgagtcttgc
     9841 agcagctaac agcataatta aaggcagggg attcggtgcc aatacgatcg atccatccat
     9901 tcaaaaagaa aaactaattc aatatggatt gagtgaggaa ttttcgagtc aattactcga
     9961 tgaagccttt tttgaagagt tgctcacaaa cgatatttat cagttttgcg caaatgtcca
    10021 taattaatct ttctcacgaa ataaaaacga tcctgccaag ttcactcgaa ctaggtaaag
    10081 atacgattca aattacatcg ctagaacaac atgtttggag tgatatcgaa aataaaatct
    10141 tatcgcgatt aaatcacctg aagtgtccac aattaatcga ttcaacatcg ttattacttc
    10201 ataagctcat ttccaatatg gtgaatacga tgcaccatag ggtatttcaa caattcgttg
    10261 aaagcgactt atacctgggt ggagtcagtg gcgataattt ttttgaagaa ctttatcaga
    10321 atgaaataga agaacatggt gacaaaaaca tcgcttccta ctgcagaaga aatgataccc
    10381 aaattacact cggcttcccg gatgatttgg aaacattatt aaccattcga taccctattt
    10441 gttgtgatag tgcagtatat ctcgacagta aactttctga agcattagga ttctctctaa
    10501 tcaaacaata tgcgggagaa gcgagtgctc attactatct tgccaagcta acaagagtaa
    10561 attttgagta tcagagttca cattttccag gatactttcc actgctatta acacgagcat
    10621 cagagttaga taaaatgaag gctattttct ctaaactcag ctatggtatt gtttgtttct
    10681 ctagtacagg tgagattcta tcgatttctt cagcgattct cgatagttta aaactcgata
    10741 gctcttcatt atcaattcaa acttttagca aaattatccc tccgcatttt tataacgatg
    10801 ttatttgggg gttagcgcta gaaacaacag aagggatttt tgagaattat agggtacgaa
    10861 ttaagcttcc aaacgattct ggtcaatcta ttcttttcaa tatcagtggt tatcgtagtc
    10921 atgattcaac cattcaatca ttatggcaag ttgtatttct tgacccaaaa tcagttgatt
    10981 cactaactga aggatcaata ttaaatgaag caagaatcca caatattact cgaaattacg
    11041 tgccacaact tgtagagcaa aaagcgcgcg atgtagtaag actgggcgga aaagaattaa
    11101 ccaatgaaga atgctatatc acggttttat tttgcgatat tgttaacttt actgcttatg
    11161 ttgaaaacaa cgaaaaagaa gaatcagtta tacatacact taatctggtt ttaggaagaa
    11221 tatctagaac tgtcaatcaa tttgagggat caattgataa attcatggga gactgcgtca
    11281 tggttttatt caaaaatcca ttaaatgctg ttctagcagc gctcgaaata cagaagcatt
    11341 cacttgatat aaatagttta agaaagaatg cagggaaaga aatgttgcta ctgagaatag
    11401 ggattcattg ggggcaagtt atcattggta acattggcat ttcaggtagg cttgattgga
    11461 ctacaattgg cgatgtagtc aatactgcgt cacgcctaga aaagaattgc caaccaggag
    11521 ctgtactaat cagccaaata ctgtatgaag ttattgcaac agtcgagcag cctggtatta
    11581 a
//
LOCUS       DAOQLJ010000067        11458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2190_length_11458_cov_6.906779, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000067 DAOQLJ010000000
VERSION     DAOQLJ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 11458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11458
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2190_length_11458_cov_6.906779"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..415
                     /locus_tag="PK522_06165"
     CDS             227..415
                     /locus_tag="PK522_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41353.1"
                     /translation="MKIKCFFTILTCAVLVACGKPSAPETPNPEEYGKTIQPFHQIPA
                     DAQEGGGKARSVEEKSSY"
     gene            complement(470..2263)
                     /gene="mutL"
                     /locus_tag="PK522_06170"
     CDS             complement(470..2263)
                     /gene="mutL"
                     /locus_tag="PK522_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNC41354.1"
                     /translation="MNVIKLLPDELINQIAAGEVIERPASVLKEVLENCVDAGATEID
                     IHLMQGGLKLIRVTDNGVGISKEDLPYALTRHATSKISNQEDLQKIVSLGFRGEALAS
                     IASVSRLSLTSFQVGNQHAWEIQAEGSQTIALQPAAGSLGTSLEVRDLFFNIPARRKF
                     LKSESTEFAHCEAIFQRIALSNPTITFNLYHNGKLRSHLASADFPERIKQLLGQEFRQ
                     TALFITEEAADIQLQGLISRPTFSRTTREMQYFFVNGRFVKDKLLNHAIREAYRDVLH
                     LDRHPAYVIYLHINPDNVDVNVHPTKIEVRFRDSRAVHQFIFHAIHKALANGHTESSE
                     ADLPDSKHHIGVVTRNIKKIPLAYSQNRQVNLPLQSAAQPSVFYQTLFGEKSHEIIAA
                     PDLAVQSESHASNFLGYALGQLLGIYILAQSNQGLIIVDMHAAHERIVYEKLKQALDQ
                     QALPTQSLLIPHLLQADALDITTVEENALLLNQLGFEISITSPTTLAIRTMPSILKNA
                     DISKLISKLLEDIRSYGSSQLLTGNRNEILATMACHSAIRANELLTIEEMNVLLREME
                     KTERSDQCNHGRPTWLALSLEQIDKLFMRGN"
     gene            2431..3090
                     /gene="rpiA"
                     /locus_tag="PK522_06175"
     CDS             2431..3090
                     /gene="rpiA"
                     /locus_tag="PK522_06175"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNC41355.1"
                     /translation="MTQDEQKRAAAAAAIQYVPVGSIVGVGTGSTANYFIDELAKIKH
                     KIEGAIASSNVTAQRLKDHGIEVLDLNTVTDLPVYIDGADEITKHLHMIKGGGGALTR
                     EKIVSAVARKFICITDQSKLVNVLGTFPLPIEVIPMARSYVAREITYLGGHPALRQDF
                     VTDNGNIILDVHGLQILNPVELETTLNQITGVVTNGLFARKAANILLLGTDHGVEMIT
                     V"
     gene            3171..3878
                     /gene="phoU"
                     /locus_tag="PK522_06180"
     CDS             3171..3878
                     /gene="phoU"
                     /locus_tag="PK522_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNC41356.1"
                     /translation="MVNKEHISKRFDADLEEVRTRVLQMGGIVEEQIEQALEALIGGN
                     EATIDQVIANDHRVNAMEVSIDEICSQIIARRQPTASDLRMIMTVIKTITDLERIGDE
                     AAKIARMARLIYAADRSHTPRFVEIKHVAEIALDMLHQALDAFARLDLNAAAHIVRQD
                     EQVDEEFRAILRQLITFMMEDPRKISTCLEILFVAKAIERIGDHAKNMSEYVVYMVKG
                     KDVRHVTADEIEREVRG"
     gene            3930..5432
                     /gene="ppx"
                     /locus_tag="PK522_06185"
     CDS             3930..5432
                     /gene="ppx"
                     /locus_tag="PK522_06185"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNC41357.1"
                     /translation="MREYSTLAAVDLGSNSFHLQVARVVDKQLYPLDSLKEMIRLAGG
                     ITADKKLDESSQERALDCLKRFGERLRGFPTHAVRVVGTNSLRVAKNAAAFLRKAEGA
                     LGFPIEIIAGHEEARLIYLGVAHALPASDNNRLVIDIGGGSTEFIIGHQLNPYKLESL
                     YMGCVSHSLHHFPDGKLTKSNMKRAELAAQTEIQSIKADFSSRHWQEAYGSSGTARAL
                     GRILMLNQFGDSLSTEEITLKGLEQFREYLLKVGDLKKVNLEGLHSDRTPVIAGGLAI
                     MSAAFTELGIDRMYLTTGALRQGVLYDLLGRSYNDDMREVSVKQFMQRYLIDTAQANR
                     IELLAGELGKQLLSDFSDEEEVEDALQLLSWAARLHEVGISVAHSSYHKHSSYILGNA
                     DMPGFSNMEQAQLSELVLSHQGSLTKVSEFLTNPINLAQSIALRLATLFYRSRMNIEL
                     PQIKLEIKGHQCRLIISQVWLDQNPLTETLLQAEIDAWKKVDWDFRIISA"
     gene            5515..6432
                     /gene="glyQ"
                     /locus_tag="PK522_06190"
     CDS             5515..6432
                     /gene="glyQ"
                     /locus_tag="PK522_06190"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNC41358.1"
                     /translation="MTPLTFQEMILALQNYWSRQGCALLQPYDMEVGAGTSHTATFLR
                     AIGPEPWRAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVVLKPAPQTILDLYLGSLQEL
                     GFDLTCNDVRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGINCKPITGEI
                     TYGLERLAMYLQGVENVFDLVWTKGLTYRDVYHQNEVEQSIYNFEQSDTAFLLLAFGK
                     YEQQANHLMENQLPLPAYEQVLKAAHSFNLLDARGAISVTERAAYIGRIRNLARRVAQ
                     AYYDSRERLDPPFPMAPREWVEQMSCKAG"
     gene            6435..8612
                     /gene="glyS"
                     /locus_tag="PK522_06195"
     CDS             6435..8612
                     /gene="glyS"
                     /locus_tag="PK522_06195"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNC41359.1"
                     /translation="METRNLLIELLVEELPPKSLKKLGESFARALSQALAMQQLTSFN
                     TIITPYATPRRLAVHLTEVLSQSPNQQQSHKLMPVAIGLDSAGQATPALIKKMSSLGM
                     EIFSVAELKRAMDGKAEALFLERTVTGTLLVNGLQTALEQAIAQLPIPKVMAYQLADG
                     WSTVNFVRPVHSLIALHGKEVVAINLLGLTAGNTTRGHRFEAKSNSIVIQEADSYAQQ
                     LQNEGAIVASFVERRQAISDQLIATANKVNLNLIADEALLDEVTALVEHPNVLMGQFA
                     EAFLEVPQECLILTMKANQKYFPLFDQHGKLTNQFLLVSNICPDDPHAIIDGNERVVR
                     SRLADAKFFFDQDRKKTLILRLPGLNAVIYHHQLGTQGLRIEYLCALASKIGAMIGGD
                     QLAIEAAQATKLAKADLLTDMVGEFPELQGVMGRYYAQHEGLSDAIAYAIEDHYKPRY
                     AGDVLPRNLIGVCVALADKLETLVNLFAIGQIPTGDKDPYALRRHALGVIRMLIEKDL
                     PIELNKLLQLVFDIVQDEAIAQQWQQAMVDEQRSVVIPKRPILTTGIVESLQQFISDR
                     LAGVLREQGYTAQEVDAVLALNPQYLNDIPKRLAAVRAFSSLPEAASLAAANKRVSNI
                     LKKVEHEISDRVNSNQLCEEAEKALHEALLDLTDKVDQAFNEKDYVVTLQILAELKTV
                     IDAFFDHVMVNVEDGDLRANRLALLKQLQQIMNRVADISRLAS"
     gene            8614..9147
                     /gene="gmhB"
                     /locus_tag="PK522_06200"
     CDS             8614..9147
                     /gene="gmhB"
                     /locus_tag="PK522_06200"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HNC41360.1"
                     /translation="MKLIVLDDKGVINEINDPFIQTPEQWVPIPGSLEAIAKLTHQGY
                     RVVVATNQSGIGRGLLEMDTFNAINDKLFKAVQAAGGYLDALFFCPHAQQEHCHCRKP
                     QIGLFKEIIQRYSTDLKDVPVVGDSLKDLQAASSIGATPILVLTGNGQQTQATNNLPT
                     GTQVYPDLASVANALLK"
     gene            9144..9947
                     /locus_tag="PK522_06205"
     CDS             9144..9947
                     /locus_tag="PK522_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC41361.1"
                     /translation="MSRMLAILRSLVYTVLQIVITPPYAIFTLSCFWLPPHQRYQVTY
                     GWTRIMLFLLKTICGLHYRIIGAEHIPKQPSIVLSKHQSAWETLAFQQIFPPQVWVLK
                     KELLRIPFFGWGLAMTSPIAIDRGSGKKALQQIVDQGKDRLAQQFWIVIFPEGTRIPP
                     GHIGKYHIGGAWLATHTGALVVPVAHNAGEFWGKNSFVKNPGTITVSIGKPIDPAGLE
                     AGELNDQVKAWIEAEVSRISQFKKIEKQNEDSGNTCSTSKQSNALHRSK"
     gene            9883..10638
                     /locus_tag="PK522_06210"
     CDS             9883..10638
                     /locus_tag="PK522_06210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNC41362.1"
                     /translation="MKIRVTRVQHQNNPTHCIVLNNQTITYSLVRRNRKTFGLRINAS
                     GLTISAPLRIPQREIEAILQRKADWIIEKLDAWENSSSNHLEWRLHAVYWLLGEPYQL
                     IALSAQLQLVPQNSIKPDMVRPCSAESSSTQFSAQQLEHFVLAWYQQQATACMTDRLA
                     FYAEKLGVGMPVFHLSNARSRWGSCNASGVIRLNWRLIQLPLALIDYVIAHELAHLFE
                     MNHSQAFWEVVAKIYPEYRRAQRELKQIGKLMI"
     gene            10790..11311
                     /locus_tag="PK522_06215"
     CDS             10790..11311
                     /locus_tag="PK522_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HNC41363.1"
                     /translation="MKLHYKIATLACALLPFYAFAGGNPDHVKLPGNYQQIFTQYDKS
                     NRANQTQVAKFFANEIAVDSYKRGEEAAPGSIVVMEIYEPRKDTEGKIIAASTGIFEI
                     EKLVAVAVMEKRADWDKSFSPDDRTGNWGFAVYSPDGVAKSNDLNCVQCHTPLKDQDY
                     LFSYGKLVNFTKK"
BASE COUNT         3288 a         2266 c         2663 g         3241 t
ORIGIN      
        1 attcgcatga attcgaacat ttatagggcc cctaagcgtt ttgcaggacc gactagttag
       61 cctaggttgg aaccgtgtaa atctcctttg tttttattga gaatgccaag tttttctttg
      121 aaatttgatt aagatcaatg tttcacaaaa aatttatggc tactattgac tggtagtttc
      181 ttataacaac atcaattact agcgagtgag ctaaaggggt ttttttatga aaattaaatg
      241 ctttttcaca atcttgacat gtgcagtatt agtagcttgt ggtaaaccat cagcaccaga
      301 gacgcctaat cctgaagagt atggaaaaac gattcaacct ttccatcaaa ttccagcaga
      361 tgcccaagaa ggtggcggta aggcaagatc ggtagaggaa aaatcgagtt actaagaggg
      421 ggaattgttc tatagcctgg cctataaggg tggggttata gaacaactat taatttccac
      481 gcatgaatag tttatcaatc tgttctaggc ttaaagcgag ccaggttggc cttccgtgat
      541 tgcattgatc ggaacgttca gttttttcca tctcacgcaa aagtacgttc atttcttcta
      601 tagttagtag ctcattggct ctaattgcac tatgacaggc catggttgct agtatttcat
      661 tgcgattacc tgttaataat tggctagaac catatgagcg gatatcctct aatagcttgc
      721 taattaattt tgaaatatca gcgtttttta agatagaagg catggtacgg atggctagcg
      781 tggtgggaga agttatcgaa atctcgaatc cgagttgatt taatagcagt gcattttctt
      841 cgacagtcgt aatgtcgagc gcatcagctt gcaataaatg gggtatcagt aaagattgtg
      901 tcggtaacgc ttgttgatca agcgcttgtt taagtttttc ataaacaatt ctttcatgcg
      961 cggcatgcat gtcaacaata atcaaacctt gattactctg ggctaaaatg tagataccca
     1021 acagttgtcc taacgcatat cctaagaaat ttgatgcgtg agactctgat tgtacggcta
     1081 gatcaggtgc tgcaataatt tcatgcgatt tttcaccaaa tagtgtttgg tagaatacgg
     1141 agggttgtgc agcagattgc agtggtaaat tgacttgccg gttttgacta taggctaggg
     1201 gtattttttt aatgttgcgt gtaacgacac caatatgatg tttggagtca ggcaaatccg
     1261 cttcgctcga ctcggtgtga ccattagcca gcgctttatg gatagcgtga aaaataaact
     1321 gatgcactgc acgtgaatcg cgaaaacgta cttcaatctt agtggggtgg acattgacat
     1381 caacattatc tggattgata tgaagatata tcacatacgc tggatgacga tctagatgaa
     1441 gcacatcacg ataagcttca cggatggcat gatttaatag cttgtctttg acaaaacgac
     1501 catttacaaa aaaatactgc atttcacggg tggtccgtga gaaagtcggt ctggatatca
     1561 acccttgaag ttggatatct gcggcttcct cagtaataaa aagtgcagtt tgcctaaatt
     1621 cctggcctag caactgttta atacgttctg gaaaatctgc acttgccaag tgacttcgca
     1681 attttccatt atggtataga ttgaaggtga tagttggatt ggataaagca atgcgctgaa
     1741 aaatcgcttc acaatgagcg aattcagttg attcggattt taaaaatttt cgccgagctg
     1801 ggatattgaa aaataaatcg cgaacttcca gcgaagtacc taatgatcct gctgcaggct
     1861 gcagtgcaat tgtttgactt ccttctgctt ggatttccca agcatgctga ttaccaactt
     1921 gaaaactggt tagcgacagg cgggatactg aagcaatgct agctagcgct tctccacgaa
     1981 aacccaagct aacgatcttc tgcagatctt cttggttact gattttgctg gttgcatggc
     2041 gagtcagtgc ataaggaaga tcttcttttg atatgccgac tccattgtca gtgacacgaa
     2101 tcagcttaag cccgccttgc atcagatgaa tatcgatctc tgtagcgcca gcatccacac
     2161 agttttcaag aacttctttg agaacagaag cgggccgttc gatgacctca cccgctgcga
     2221 tttgattgat taattcatcc gggagcaatt taatgacatt catactacaa aatcaacacg
     2281 gctgtcgttc atagagttat gatataaagt ttaatcgact aacttaatca ttttaaacca
     2341 atacgagtta gttcggattt ctatagcatg atcgatagac tcatcgctaa tatactatct
     2401 attaattatt gatctcgaaa taggaacaac atgacgcaag atgaacaaaa acgggctgct
     2461 gccgctgccg caatccaata tgtcccagta ggctcgattg tcggtgtagg tacaggttct
     2521 accgcaaatt atttcatcga cgaactagca aaaattaagc acaaaattga aggagcgatt
     2581 gctagctcaa atgtaacagc acaacgcctc aaagatcatg gaatcgaagt gcttgacttg
     2641 aataccgtta cggatttgcc tgtttatatc gatggagccg atgaaattac caaacatttg
     2701 cacatgatta agggaggcgg aggtgctttg acacgagaaa agattgtttc agccgtcgct
     2761 agaaaattta tttgtatcac cgatcagagc aagttagtaa atgtattagg gactttccca
     2821 cttcctatcg aagtcatacc gatggcacgt agctatgttg cccgtgaaat tacatattta
     2881 gggggacacc ctgctttgag gcaagatttt gtgacggaca atgggaacat tatcttggat
     2941 gtgcatggtt tgcaaattct taatccagtt gagcttgaaa caacgctcaa ccaaatcaca
     3001 ggtgtggtga ctaacggctt atttgcacga aaagcggcta atatcctatt gcttggtact
     3061 gaccatggtg tggagatgat tacggtttga tgctgtgctt tattttgtca tgaaatggtt
     3121 atcaatgtga ccgacaattg gtgattctga tcactcaaaa aggataacat atggtcaaca
     3181 aagaacatat atcgaaaaga tttgatgccg atctcgagga agttcgaacc cgcgtgctgc
     3241 aaatgggcgg aatcgttgaa gagcaaattg agcaagcgct cgaagcattg atcgggggca
     3301 atgaagccac aatcgatcag gtgattgcga atgaccatcg ggtcaatgca atggaagtct
     3361 cgattgatga aatttgcagt caaattattg cacgtcgcca accaaccgcg agtgacttgc
     3421 gtatgatcat gactgtcatc aagacaatta ccgatcttga gcgaatcgga gatgaggcag
     3481 cgaaaattgc acgtatggcc aggctcattt atgcagcaga tcgttcacat acgcctcgct
     3541 ttgtggaaat aaagcatgtg gctgaaattg cactcgatat gcttcatcaa gcgttggatg
     3601 cattcgcaag attggatttg aatgctgcag cgcacatcgt ccgtcaagat gagcaagtcg
     3661 atgaggagtt tcgtgctatt ttgcgtcaac tgattacctt tatgatggaa gatccgcgga
     3721 aaatttcaac gtgcttagaa attttatttg ttgcaaaagc cattgaacga attggggatc
     3781 atgccaaaaa tatgtcggaa tacgttgtgt atatggttaa aggaaaggat gtgcgccacg
     3841 ttactgcaga tgaaattgaa cgggaagtgc gtggatagca aaatagatat caatgctagc
     3901 tagttttagc tatcagcaaa tttaaagtga tgcgtgaata ctcaaccttg gccgctgtcg
     3961 atttaggctc aaatagcttc cacttgcaag ttgcgcgtgt tgtcgataaa caactttacc
     4021 cattagatag cttaaaagaa atgatccgtc tggcgggcgg cattacagcg gataagaaac
     4081 tcgatgaatc gtcgcaagaa agagcgctgg attgtttaaa acgttttgga gagcggttgc
     4141 gcggttttcc cacccatgca gttagagtgg taggcactaa ttctctacgc gttgctaaga
     4201 acgctgctgc gtttctaaga aaagcagaag gcgctttagg atttccaatt gaaatcattg
     4261 cgggtcatga agaagctcgg ctaatttacc tgggggtggc gcatgcatta ccagcttcgg
     4321 ataataatcg acttgttatc gatattggtg gtggttcaac ggaattcatt attggtcatc
     4381 aactgaatcc ctataagtta gaaagtctgt acatgggttg tgtcagccat agccttcatc
     4441 attttcccga tgggaagttg actaaaagca atatgaagcg tgctgaactg gctgctcaaa
     4501 ccgaaataca aagtattaaa gctgattttt cttctcgtca ctggcaagaa gcctatggtt
     4561 cgtcaggaac ggctagagca cttggacgta tcttaatgct caatcagttt ggagatagct
     4621 taagtacgga agaaattact ttaaaggggc ttgaacagtt tcgagaatat cttttgaaag
     4681 tgggtgatct caagaaagtg aatttggaag gactccacag tgatcgaaca ccagtcattg
     4741 ctggagggct tgccatcatg tctgctgcgt tcactgaact cggcatcgat cggatgtatt
     4801 tgactaccgg tgcgttgcgg caaggggttc tatatgatct gttaggccgc tcatataacg
     4861 atgatatgcg tgaggtttcg gtcaagcaat tcatgcagcg ttatctcatt gatacggcgc
     4921 aggccaaccg aattgagcta ttggcgggcg aactgggtaa acagctatta tcagattttt
     4981 ctgatgagga ggaagtagaa gatgcacttc aattgctttc ttgggcagcg cggttacatg
     5041 aagtagggat ttcagttgcg cattccagct atcacaaaca ttcatcatat atcctgggta
     5101 atgctgacat gccagggttt tccaatatgg agcaagcgca gctcagtgaa ttggttcttt
     5161 cacaccaagg atcattgacc aaagtcagtg aatttttaac aaacccaatc aatttggctc
     5221 aatcgatcgc gctaaggctt gctacattat tttaccgaag ccggatgaat attgagttgc
     5281 cacaaatcaa gcttgaaatt aaaggacatc agtgcaggct catcatttcg caggtctggc
     5341 tcgatcaaaa tccactgaca gaaactttgt tgcaagctga aatcgatgcg tggaaaaaag
     5401 tcgattggga cttccgaatt attagcgctt gatttcttgt taattgttta attatgtagt
     5461 tttcaacatc gtagcttaca ctgcattgtt tatattttgc ttcattacgc atttatgacc
     5521 ccattgacgt ttcaagaaat gattttagcg cttcaaaatt actggagtcg acaaggctgt
     5581 gcattgctac aaccttatga catggaagtt ggcgctggaa cgagccatac agccactttt
     5641 ttgcgcgcca ttggccctga gccctggcga gccgcctatg tgcagccttc acggcgtccc
     5701 aaagatggtc gttatgggga taacccaaac cgcctgcagc actactatca atttcaggtt
     5761 gttttaaaac cggcacctca aacaattctg gatctttacc tcgggtcgtt gcaggaactt
     5821 ggttttgatc taacttgcaa tgatgtgcga ttcgtcgaag acgattggga aaatccaaca
     5881 ctgggcgctt ggggcctagg gtgggaggtt tggctaaatg gaatggaagt aacacaattt
     5941 acttattttc agcaagtcgg cggcatcaac tgtaaaccta tcacgggaga gatcacctat
     6001 ggtctggagc ggttagcgat gtatttacag ggtgtcgaga atgtgtttga tctggtctgg
     6061 acaaaggggt taacttaccg cgatgtttat caccagaatg aggttgagca atcaatttat
     6121 aactttgagc aaagcgatac cgcatttttg ctgctggcat tcggtaaata tgagcagcaa
     6181 gcgaaccatc ttatggaaaa ccaattgcca ttacctgctt atgaacaagt acttaaagca
     6241 gcccatagct ttaatttgct ggatgcaaga ggtgctattt ccgtaactga gcgtgcggct
     6301 tatattggtc gtatccgcaa tttggcccga cgggtagcac aagcatatta cgatagtcgt
     6361 gaacgattag atccaccgtt tccaatggcg cctcgtgagt gggtagagca aatgtcgtgt
     6421 aaggcaggat gaagatggaa acgagaaatt tactcatcga attgctggta gaagagcttc
     6481 cacccaagtc gttaaaaaaa ttgggggaaa gttttgcccg cgccttaagt caggcgctag
     6541 ctatgcagca gcttacgtcg tttaatacga ttataacgcc ctacgctacg ccgcgccgat
     6601 tagcagttca cttaactgaa gtattgagtc agtcccccaa tcaacagcag tcgcataaac
     6661 ttatgccagt agctatagga ctagactctg ctgggcaagc cacacctgcg ctgatcaaaa
     6721 aaatgagcag tttaggcatg gaaattttta gtgttgcaga acttaaacgc gccatggacg
     6781 gcaaagcgga agcattgttt ctagaacgga ccgtaactgg aacattgctg gtcaatggat
     6841 tgcaaacagc attggagcag gccatagcac aactaccgat ccctaaggtg atggcctacc
     6901 agctagccga tggttggagt acggttaatt ttgttcgccc cgtccattca cttattgcgt
     6961 tacacggaaa ggaagttgta gcaatcaatt tattgggact gactgccgga aataccacac
     7021 gcgggcatcg atttgaagcc aaatccaatt caattgtaat tcaagaagcg gatagctatg
     7081 cgcagcagct acaaaatgag ggtgctatcg ttgctagttt tgtagaacgt cgccaagcga
     7141 tcagcgatca gcttattgct actgcaaata aagtaaattt aaacttgatt gctgatgaag
     7201 ctttactcga tgaagtaact gctttggtag aacatccgaa cgtgctgatg gggcaattcg
     7261 ctgaagcttt cctggaagtt ccgcaggaat gtcttattct taccatgaaa gcgaatcaaa
     7321 aatattttcc gctattcgat caacacggca agctaaccaa tcaattcctg cttgtttcca
     7381 atatctgccc cgatgatcct catgcgatta ttgatggcaa tgaacgcgta gttcgttctc
     7441 gcttggccga tgcaaaattt ttctttgatc aggatcgcaa aaaaactctg attttacgcc
     7501 ttcctgggct aaatgccgtc atttatcatc atcagttggg gactcaaggt ttgcgaattg
     7561 agtacctctg tgcattagcg agtaaaattg gcgcgatgat aggaggggat caactcgcga
     7621 ttgaagctgc gcaagcaaca aagcttgcca aagcggattt acttaccgat atggttggcg
     7681 aatttcccga gctgcaaggg gtaatggggc gttattatgc acagcatgaa gggttgagtg
     7741 atgccattgc ttacgcgata gaagatcact ataaaccgcg ctatgccggc gatgtgttgc
     7801 cacggaattt gatcggtgtg tgtgttgccc tggctgataa gttagaaaca cttgttaatt
     7861 tgtttgccat tggtcagatt ccaactggcg ataaagatcc ttatgcgcta aggcgtcatg
     7921 cgctaggtgt tatccgaatg ctgatcgaaa aagatttacc cattgaatta aataaactgt
     7981 tgcagttggt ttttgatatc gtacaagatg aggcgattgc gcaacaatgg caacaagcga
     8041 tggtcgatga gcagcgttca gtagtgattc ctaagcgtcc aattttgacg actggaatcg
     8101 ttgaatcatt gcaacagttc atatcggatc gattggcagg cgtcttacgt gaacaaggtt
     8161 atactgctca ggaggttgat gctgttttag cacttaatcc acaatatttg aacgatattc
     8221 ctaaacgatt agctgctgta cgcgcatttt caagtttgcc cgaggctgcg agtcttgccg
     8281 ctgccaataa gcgggtcagt aacatactca agaaagttga acatgaaatc agtgaccgag
     8341 taaattctaa tcaactatgc gaggaagcgg aaaaagccct ccatgaagcg ctgctcgatt
     8401 taactgataa agtagatcag gcatttaatg aaaaagatta tgtcgtaacg cttcagatcc
     8461 ttgctgaatt aaaaacagta atcgatgctt tctttgatca tgttatggtc aatgtcgaag
     8521 atggggactt gcgtgctaat cgacttgcat tactcaagca gttacaacaa atcatgaatc
     8581 gtgtagcgga tatttcaagg ttagcgagtt gatatgaagc ttattgtgct tgacgataaa
     8641 ggagtaatca atgaaatcaa tgatcctttc atccaaacac ccgaacaatg ggtgccaata
     8701 cccggaagtt tagaagctat cgcaaagctt acgcaccagg gttatcgtgt cgtcgtggct
     8761 accaatcaat ccggaatcgg gcgtggtttg ctcgaaatgg atacttttaa tgctatcaat
     8821 gataagcttt ttaaagcggt tcaggcagcg ggcggttacc tggatgcctt atttttttgt
     8881 ccacacgcac aacaggagca ttgtcattgc cgcaaacctc agataggttt atttaaagaa
     8941 attattcaac gctatagcac cgatttgaaa gatgttcccg tcgtgggtga ttcattgaaa
     9001 gatcttcaag ctgcaagtag cattggagca acaccgattc tcgttctgac tggaaatgga
     9061 cagcaaacac aagcaaccaa taatctgccg actggaacac aagtttatcc ggatttagca
     9121 tccgtggcga acgctttatt gaaatgagtc gtatgcttgc tatccttcgc tctctggttt
     9181 ataccgtgct gcaaattgtc attactccac cttatgcaat tttcacattg tcctgcttct
     9241 ggttaccacc gcatcagcgc tatcaagtaa cgtatggttg gacccggatc atgctttttt
     9301 tacttaagac catttgtggt ttgcactatc gaattatcgg tgcggagcac attccgaagc
     9361 agcctagcat tgtgttatcg aagcaccaat cggcttggga aacgttagct tttcagcaaa
     9421 tctttccgcc tcaagtctgg gttcttaaga aagagctact acgtatccct ttttttggct
     9481 ggggactcgc aatgacaagc ccgattgcca tcgatcgagg ctctgggaaa aaagcgttgc
     9541 agcaaattgt cgatcaagga aaagatcggc ttgctcaaca attttggatc gtcatttttc
     9601 cagaaggaac gcgcattccg ccaggtcaca tcggtaaata tcatattggt ggggcttggt
     9661 tggctactca taccggtgca ttggttgtgc cggttgcaca taatgctggc gagttctggg
     9721 ggaagaattc attcgttaaa aatcctggaa cgattaccgt gagtattggc aaaccaatcg
     9781 atcctgctgg attggaagcg ggcgaattga atgatcaagt aaaagcgtgg attgaagctg
     9841 aggtatcgag aatcagtcaa tttaaaaaaa tagaaaagca gaatgaagat tcgggtaaca
     9901 cgtgttcaac atcaaaacaa tccaacgcat tgcatcgttc taaataatca aacgattacc
     9961 tattcattag ttcgacgtaa tcggaaaacc tttgggttga gaatcaacgc atctggctta
    10021 actatttcag cccctttaag aataccccaa agagaaattg aagccatact ccagcgcaag
    10081 gccgattgga ttattgaaaa acttgatgcc tgggaaaata gcagcagtaa tcaccttgag
    10141 tggaggcttc atgcggttta ttggttattg ggggaacctt atcagctcat tgctttatcg
    10201 gcgcaattgc aattagtgcc acagaatagc atcaaaccag atatggtcag gccatgcagc
    10261 gctgagtcat cgtcaacaca attttctgca caacagcttg agcactttgt tttagcatgg
    10321 tatcaacagc aagcgaccgc gtgcatgacg gatcgcttag cattttatgc tgaaaagctt
    10381 ggtgtaggaa tgccagtttt tcacctttcg aatgcccggt cacggtgggg tagctgcaac
    10441 gcatcaggag tgattcgcct taactggcga ttgattcagt tgccacttgc tttaatagac
    10501 tatgtgattg cccatgaatt agcacatctt ttcgagatga atcattcgca agcattctgg
    10561 gaagtggtag caaaaatcta tccagaatat cgccgggcac agcgtgaatt aaagcaaatt
    10621 ggtaagctga tgatttgaag atcgaaagat accgtgaatt tctatcgctt tatcgttcaa
    10681 aaataatgaa ataccgtgtt gtataaagaa tagatcaata ttatgcttta gtctgagcat
    10741 tgggatattt ttcacaataa ctattggttg attttgataa ggagagaaga tgaaactaca
    10801 ctataaaatt gcgactctag cttgtgcact gttacctttc tatgcgtttg caggcgggaa
    10861 tcctgaccat gtaaagttac cgggaaatta tcagcaaatc ttcacccagt atgataaatc
    10921 gaatcgtgct aatcaaacgc aagtcgctaa attttttgca aatgaaattg cggttgatag
    10981 ctataaaaga ggggaagaag ctgcgccggg ttcaattgtt gttatggaaa tttatgaacc
    11041 tagaaaagat acagaaggaa aaatcatcgc tgccagtact ggtatatttg aaattgagaa
    11101 actagtggct gttgctgtga tggaaaaaag ggctgattgg gataaatcat tttctcccga
    11161 tgatcgtact ggtaactggg ggtttgctgt atacagtcct gatggagtcg ctaaaagtaa
    11221 tgatctaaat tgtgtgcaat gccacacacc attgaaggat caagattatt tatttagcta
    11281 tggaaaatta gttaatttca ctaagaaata acaaaatatc tcgattgcag tccagattgc
    11341 agtccagatt taacttgcac tatcggacag tcgcttactg agcggattgt ccgataacga
    11401 tcagcgaaaa tcctacttaa gtttcacgaa taagcgcttg cttcaataca gcaatatt
//
LOCUS       DAOQLJ010000068        11401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2202_length_11401_cov_6.609642, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000068 DAOQLJ010000000
VERSION     DAOQLJ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 11401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11401
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2202_length_11401_cov_6.609642"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2252
                     /locus_tag="PK522_06220"
     CDS             <1..2252
                     /locus_tag="PK522_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HNC41364.1"
                     /translation="LGSLTDCDLVIEAIAERVDWKTELYAKIIPYLAPHTLVASNTSG
                     LSINQLAAALPEELQHRFCGIHFFNPPRYMHLIELIPCTYSDQTTLDHLESFLTTYLG
                     KGVIRAKDTPNFIANRVGVFSMLATIHHASAMGLSFDLVDQLTGTLIGRPKSATFRTA
                     DVVGLDVLSHVVETMRHSLAQDPWHSYYALPGWLQQLITQGALGQKSSRGVYQKKNKD
                     ILVFNPAKNEYENSTATIDEDIQQLLKQKDPARKFSELRENPHPQAQFLWAIHRDLFH
                     YCAVHLAEIANNARDLDLAVRWGFGWTLGPFEIWQAAGWVPITKWINEDIAAGRAMSQ
                     TPLPDWVMELGKTAPPGVHGTMGSFAPLTKGYQPRSTLSVYRRQPFPDRLIGEEHRYG
                     ETIFENNAVRMWHTGDDIAIVSFKSKMNTIDIEVLDGLQQAIEEAEHHWSALVIWQTE
                     PPFSAGANLQKATEKPKNDEPPSPFDQFVKKVKRTAQSTILQAARSLDLADVLMASKL
                     AEVETLITRFQQLSQHLRYSMIPTVAAVDGLALGGGCEFVMHCDRAVATLESYIGLVE
                     AGVGLLPAGGGCKELALRAAKESVDGDPFPKLKHYFQTVAMAQLGKSAEQAKELGYLR
                     QADMIVMNRFELLHVAKTQANAMAEAGYRPPLQFRDIPVAGSTGVATIKGMLVNMLEG
                     HFISEHDYLIGSKIAHVMCGGNLTPGSLVDEEWFLRLERTAFIELLATEKTQARIAHT
                     LKTGKPLRN"
     gene            2296..3495
                     /locus_tag="PK522_06225"
     CDS             2296..3495
                     /locus_tag="PK522_06225"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNC41365.1"
                     /translation="MTKQTQDAYIVAAVRTPVGKAPRGMFKNVRPDDMLVHVLQAVMK
                     QCTGLDPAAIDDVVAGCAMPEAEQGINVARVASLLAGLPDSVPGMTVNRFCASGLQAV
                     AIAADRIRLGEADVIIAAGTESMSMVPMMGNKVAMNPAMFTHNEHVAIAYGMGMTAEK
                     VAQQWKITRDQQDEFALTSHTRAINAIDMGEFDDEIAPYAIEENRPDLSSHEVITVKT
                     MRDTDEGPRADTSLDALAKLKSVFAADGTVTAGNSSQMSDGAGAVVVMSAAALKRFSL
                     EPIGRFVGYAVAGVPPEIMGIGPIKAIPKVLKQTGLKQEELDWIELNEAFAAQSLAVI
                     RDLGLDRNKVNPLGGAIALGHPLGATGAIRVATLLHGLRRHKQKYGMVTMCIGSGMGA
                     AGVFESL"
     gene            complement(3508..3687)
                     /locus_tag="PK522_06230"
     CDS             complement(3508..3687)
                     /locus_tag="PK522_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41366.1"
                     /translation="MKVMVIFKTGTSQVFIVPRDILAVEFRRLAESVGGEIHRIEFMQ
                     KNKFAAPKYALIKDI"
     gene            3881..5545
                     /gene="ettA"
                     /locus_tag="PK522_06235"
     CDS             3881..5545
                     /gene="ettA"
                     /locus_tag="PK522_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNC41367.1"
                     /translation="MAQYVLTMNRVGKIVPPKRTILKDISLSFFPGAKIGLLGLNGSG
                     KSTLLKIMAGVDKDIEGEVTPMPNLKIGYLPQEPQLNPDQTVREAVQEGLGDIASAQQ
                     KLDEIYAAYADPDADFDALAAEQTRLEAIIATGGGDTAQQMEVAADALRLPEWDAVIR
                     NLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAVTHDR
                     YFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEIRLKQEESSESARQKALQKELEWIR
                     QNPKGRQAKSKARIARFEELNSLEYQKRNETQEIFIPVADRLGNEVIELMDVSKAFGD
                     RLLIDNLSFKIPPGAIVGIIGPNGAGKSTLFRMIMGKEKPDSGEIKIGETVKVAYVDQ
                     SRDVLESDKTVFEAISGGNDILVVGKYETPSRAYIGRFNFKGPDQQKIVGQLSGGERG
                     RLHLAKTLISGGNVLLLDEPSNDLDVETLRALENALLEFAGCVLVISHDRWFLDRIAT
                     HILAFEGDSQVTFFAGNYQEYESDKRQRLGGEGAKPKRIRYKPIAR"
     gene            5657..5887
                     /locus_tag="PK522_06240"
     CDS             5657..5887
                     /locus_tag="PK522_06240"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HNC41368.1"
                     /translation="MGKWLVLLIVIWFIFWVLKSLQRLKAKTHHNSIDGIEDMVQCRY
                     CEIHIPKKEAIVADNHFYCCSEHYQLHSQSDT"
     gene            complement(5888..6913)
                     /locus_tag="PK522_06245"
     CDS             complement(5888..6913)
                     /locus_tag="PK522_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNC41369.1"
                     /translation="MQKPIAIWLFICCALVFAMVVVGGVTRLTGSGLSIVEWQPLIGA
                     IPPLSEADWEILLEKYRQIPQYHEVNKGMTLEEFKGIFWWEYFHRLLGRAIGLVFFIP
                     FLYFLIRKQIDRPLGLKLLGIFVLGGLQGLMGWYMVMSGLVNNIYVSQYRLTAHLGLA
                     FIIYAAMFWVATGLLSPAKKQQQPETESYYSHLRRYSFYLTLLIFIMILSGGFVAGIH
                     AGKAYNTFPLMDGHLIPPGLFVLEPWYRNFFENITTVQFDHRLIAWLLAFFVPFFWLR
                     CIKYSLSPSAKMACHLLLLALILQISLGVATLLSAVPLTLAATHQAGAMVLFTASLWV
                     NRKLSSI"
     gene            complement(6950..7393)
                     /gene="smpB"
                     /locus_tag="PK522_06250"
     CDS             complement(6950..7393)
                     /gene="smpB"
                     /locus_tag="PK522_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNC41370.1"
                     /translation="MSIAQNKKAFHDYFIEEKYEVGIVLEGWEVKSIRAGRVQLKEAY
                     VIIRNNELYLIGSHISALPTASTHINPDPLRTRKLLLHAEQIKRLIGKVERAGYTLVP
                     LDMHYKTGRIKLEIGLAKGKKQYDKRESEKQKEWDRTKQRLLRAR"
     gene            7491..7931
                     /locus_tag="PK522_06255"
     CDS             7491..7931
                     /locus_tag="PK522_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HNC41371.1"
                     /translation="MAEIKKVVYVDYSAGQMFRLVDTVENYPEFLPWCDGTSVKQLSD
                     TIVHATVNINYGPVRHSFTTENTRQVPELIKMKLLDGPFEELDGEWRFISLSEHECKI
                     EFNLHYTFSNRLLEKLVGPVFFGIANSFVDAFIKRAEKIYGISQ"
     gene            7915..8229
                     /locus_tag="PK522_06260"
     CDS             7915..8229
                     /locus_tag="PK522_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HNC41372.1"
                     /translation="MAFLSEKNIEVEIAYALSRHQFLQRITVPSGITVEQAITLSEFD
                     KRFPDIDWVSQKIGIFGKLTTLDAVLQNNDRVEIYRPLQCDPKENRRIRYKKKTVRLS
                     GI"
     gene            8267..8569
                     /locus_tag="PK522_06265"
     CDS             8267..8569
                     /locus_tag="PK522_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNC41373.1"
                     /translation="MLYVINGEDVPNSSEKRLIARPGHLERLQVLQQEGRLVLAGPYP
                     AIDNVDPGPAGFSGSLIVAEFESLEVAQAWANADPYVLAGVYLHVTVKPFKKVFPT"
     gene            8707..9495
                     /locus_tag="PK522_06270"
     CDS             8707..9495
                     /locus_tag="PK522_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNC41374.1"
                     /translation="MYLRKFLLLMLVMITGLVITSSLFAQTVAKVNGVGIPQARLDFM
                     VKAAIAQGQQDSTELRNSLRENLIAEEIVAQEAIKSGLDKSSDVMTQIELARQAILVR
                     AYQAEYIKNNAVGDDVLRREYEEVKSQMGDKEYKARHILVETEKEAKDIIAALKKGGA
                     FDKLAKEKSIDTGSKSNGGELGWSSAGAYVKPFADALQNLKKGNTTDQPVQTSFGWHV
                     VRLEDVRPAVAPPFDEVKMNMQQRVLQRNFASTIQNLRNSAKVE"
     gene            complement(9545..10624)
                     /gene="alr"
                     /locus_tag="PK522_06275"
     CDS             complement(9545..10624)
                     /gene="alr"
                     /locus_tag="PK522_06275"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNC41375.1"
                     /translation="MSRPISAQINLAALRHNLSIVRQYAKHSRIMAVIKANAYGHGLI
                     RVAKTLHEIDAFALLELEAAIQLREIGIHQPILLLEGFFSEADLISIDHYQLDTVIHH
                     PEQIAMLAKHRANKKINLFLKINTGMNRLGLAPREGTKAIHTLKNLSHVGEITLMTHL
                     ACADDPAADREINDQLNIFSEILNITRLPCSLANSAAIIQHPQTHQDWVRPGIMLYGA
                     SPLIHKTATELDLKPVMTVSSKIIALQQLNTNDKVGYGGTFRAQQPMQIGIVAAGYAD
                     GYPRHAQTGTPIIVNGQRTRIVGRISMDMLTVDLTGITNVTVGSPVILWGNHLPIEEV
                     AKSANTISYELFCSLSSRVKVITTE"
     gene            10783..>11401
                     /locus_tag="PK522_06280"
     CDS             10783..>11401
                     /locus_tag="PK522_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signaling recognition particle receptor family
                     protein"
                     /protein_id="HNC41376.1"
                     /translation="MFSFFKSQKNPPEDQSQSVSFVSKIKQGLARTRENLGKQLSSLF
                     GARKIDEAVYEELETILLTADVGIQATNQLLDSLRGKVKRDQLSDTHQLKGVLQETLI
                     ELLQPLARPLDISTHQPFVIMIAGVNGVGKTTTIGKLAYYFQSQGKSVLLAAGDTFRA
                     AAREQLVEWGKRNNVTVIAQESDSPGKSDPAAVIFDAINAAKARNI"
BASE COUNT         3364 a         2104 c         2678 g         3255 t
ORIGIN      
        1 atcttggttc gttgacggat tgtgacttag taattgaggc cattgcagag cgcgtcgatt
       61 ggaaaaccga gttatacgca aagataattc cttatcttgc accgcatact ttggtagcaa
      121 gtaatacttc tggactttcc attaatcagc tcgcagcagc gctgccggaa gaattgcaac
      181 atcggttttg tggaattcat ttttttaatc caccccgcta catgcatttg atcgaattga
      241 ttccttgtac ctatagtgat caaacaacac tcgatcatct cgagtccttt ttaaccactt
      301 atcttggtaa aggggtgata cgggctaaag atacacctaa ttttattgca aatcgagtgg
      361 gtgtgttttc gatgttggca acgatacatc atgcaagtgc aatgggctta agctttgatt
      421 tagtagatca gctcacggga acgctcattg gccgtcctaa aagtgctaca ttccgaacag
      481 ccgatgttgt gggattagat gttttatcgc atgtggtaga gactatgcgc cattcactag
      541 cgcaggatcc ttggcatagc tattatgctc ttccgggatg gttgcagcaa ttgattacac
      601 aaggtgcgct tggtcaaaaa tcgagccgtg gcgtgtatca gaaaaaaaat aaagatattt
      661 tggtatttaa tcctgcgaaa aatgaatatg aaaattcaac tgctacgatc gatgaggata
      721 ttcaacagtt actcaaacaa aaagatcctg ctagaaaatt ttctgaatta agggagaacc
      781 ctcatccgca ggctcaattt ttgtgggcga ttcatcgcga tttatttcac tattgtgccg
      841 ttcacctagc agaaattgct aataatgcgc gtgatctgga tcttgctgtg cgttggggtt
      901 ttggctggac attgggtcct tttgaaattt ggcaagcagc tggttgggta ccaatcacga
      961 aatggattaa tgaagatatt gcggctggtc gtgccatgag tcagactccc ttgcctgatt
     1021 gggtaatgga attgggtaaa actgcgcccc cgggagtcca tggtacaatg ggttcttttg
     1081 cgcccctaac aaagggatat caacctcgat ctaccttatc cgtatatcgg cggcagccct
     1141 ttcctgatcg actgattggc gaagaacatc gctatggaga aacaattttt gaaaataatg
     1201 cagtcaggat gtggcacact ggagatgata tcgccattgt tagttttaag agcaaaatga
     1261 atacaatcga tatcgaagtt ttggatggtt tgcagcaagc gattgaagag gcagaacacc
     1321 attggagtgc cctcgtcatt tggcaaacag agccaccttt ttcagcgggt gctaatctac
     1381 aaaaagcaac tgagaagccc aaaaatgatg agcctccctc accatttgat caatttgtaa
     1441 aaaaagtcaa aaggactgca cagtctacga ttttacaggc cgcacgtagt ttagatctag
     1501 cagatgtact tatggctagc aaactcgctg aagtggaaac gctcattaca cgctttcagc
     1561 agctatcaca gcatttacgc tactcaatga tcccaactgt tgcggcagta gatggacttg
     1621 cgcttggggg cggttgtgag ttcgttatgc actgtgatcg agctgttgcc acattggaaa
     1681 gttatattgg cctggtggaa gcgggcgttg ggttgctacc tgcaggagga ggatgcaagg
     1741 aattggcatt aagggccgct aaagaatcgg ttgacggtga tccatttcct aaattgaaac
     1801 attattttca aacagtggcg atggctcaac ttggaaaatc tgcagaacaa gccaaagaat
     1861 taggctattt acgccaagct gacatgattg tcatgaatcg gtttgagttg ctgcatgtcg
     1921 caaaaacaca agctaatgcg atggcagagg caggttatcg tcctccattg caatttcgtg
     1981 atattcccgt cgcaggatca actggggttg caacgatcaa agggatgctg gtgaatatgc
     2041 tggaagggca ttttatctct gaacatgact acttaattgg gagcaaaatt gctcatgtca
     2101 tgtgtggtgg caatctaacc ccagggagtt tggttgatga ggagtggttt ctgcgattag
     2161 aacgtacagc atttattgaa cttctagcga ctgaaaaaac ccaagcccgg attgcacata
     2221 cgctgaaaac cggcaaacca cttcgcaatt agcgataaaa tagtgaagcg attattcgtt
     2281 taatggagaa aagcgatgac taaacaaact caggatgcct acattgttgc tgccgttcgc
     2341 acacccgttg gtaaagcgcc ccgtggcatg tttaaaaatg tgcggcctga tgacatgctt
     2401 gtccatgtat tgcaagcggt tatgaaacaa tgtactggtc ttgatcctgc tgccattgat
     2461 gatgtggttg ctggttgtgc aatgcctgaa gcggaacaag gtatcaatgt ggcgcgtgta
     2521 gcatcgttac ttgctggatt gccggatagt gtgccgggaa tgacggttaa tcgcttttgt
     2581 gcctcagggc ttcaagcggt tgcgatcgct gcagatcgca ttcgtcttgg agaagcggat
     2641 gtgatcattg ctgcaggaac agaaagtatg agcatggttc ctatgatggg caataaagtt
     2701 gcgatgaatc ctgcaatgtt cactcataat gagcatgttg caattgccta tggaatgggt
     2761 atgactgctg agaaagttgc tcaacagtgg aaaattaccc gtgatcagca agatgaattt
     2821 gcactaacta gccatacgcg tgctatcaat gcaatcgata tgggcgaatt cgacgatgaa
     2881 attgcgccct acgccattga ggaaaataga cctgatctgt catcacatga agtcatcaca
     2941 gttaaaacga tgagagacac ggatgagggg ccgcgcgcag ataccagttt agatgccttg
     3001 gcgaagctga agtcagtatt tgcagcagat gggactgtta cggctggtaa cagttctcaa
     3061 atgtcagacg gtgcaggtgc cgtggttgtt atgagtgctg cagctctaaa acgatttagc
     3121 cttgaaccca ttgggcgttt cgttggctac gcggttgctg gcgtaccacc ggaaataatg
     3181 ggaatagggc cgattaaagc tattcctaaa gtattaaaac aaacgggatt gaagcaagag
     3241 gagctcgatt ggattgaatt aaatgaagca tttgctgcgc agagtctggc agtgattcgt
     3301 gatttaggac tggatcgtaa taaagtcaat ccattagggg gagcaattgc actcggtcat
     3361 ccgcttggtg caactggcgc aatacgagtc gcgacgctgt tacatgggct gcgtcggcat
     3421 aaacaaaaat atggaatggt tacgatgtgt atcgggagcg ggatgggcgc tgctggcgtt
     3481 tttgaatcat tatgaattcg ttgaatctta aatatcttta attaatgcat atttaggagc
     3541 agcaaattta ttcttttgca taaattcgat gcggtggatt tcaccaccga ctgattctgc
     3601 caatcgtctg aattcaactg ctagaatatc gcgaggaacg ataaatacct gactggtgcc
     3661 cgttttaaaa attaccatta ccttcataac tttctcctag ttagcgctta ttcgtaaaaa
     3721 tgtttagcgg ttaatcgctt aaaaacaatt ttggatcggt gcaacgtata aatgtatggt
     3781 tatattcggc atctcattac ttatcgatta acagataata gaattttaga taaatttcct
     3841 aaatttaaac ttttaataac tcacactttt acccatattt atggctcaat atgttttaac
     3901 gatgaatcgt gttggcaaaa ttgttccgcc taaacgtacg attcttaaag atatttctct
     3961 tagtttcttc ccaggcgcga aaattggctt gcttggccta aatggctcgg gtaaatcgac
     4021 tttgcttaaa attatggctg gtgttgataa agacattgaa ggtgaagtca caccgatgcc
     4081 taatttaaaa atcggctact tgcctcagga acctcaactt aatccagatc aaacggttag
     4141 ggaagctgtg caggagggat taggtgatat cgccagtgcg caacagaaat tagatgaaat
     4201 ctacgcggct tatgccgatc cggatgctga tttcgacgca ttagcggcag agcaaacacg
     4261 attagaagcc attattgcaa cgggtggcgg tgatacagca caacaaatgg aagtcgctgc
     4321 tgacgcatta cgtctgcctg aatgggatgc agtgattcgt aacttgtctg ggggagaaaa
     4381 gcggcgggta gcgctgtgtc gcttattact ttcaaagccg gatatgttgt tgctcgatga
     4441 acctacgaat cacttggatg cagaatcagt ggaatggcta gaacaatttt tggtccgatt
     4501 tccggggact gtagttgcgg taacccatga tcgttacttt ctcgataacg cagcagagtg
     4561 gatattggag cttgatcgtg gccatggaat tccttggaag ggtaattatt ccagctggct
     4621 agagcaaaaa gaaatccgcc tcaaacaaga agaatcgtca gaatcggcgc ggcaaaaggc
     4681 attgcagaaa gagctggagt ggattagaca aaatcctaag ggacgacaag ctaaatcaaa
     4741 agctaggatt gcgcgttttg aagaactcaa ttctttagag tatcagaagc gtaacgagac
     4801 tcaggaaatt tttattccag tagccgacag acttgggaat gaagtgatcg agttgatgga
     4861 tgtttctaaa gcttttgggg atcgactgct gattgataat cttagtttca agattccacc
     4921 cggtgccatt gttggcatca ttggccccaa tggtgcagga aaatcaacct tgtttcgcat
     4981 gatcatgggc aaagagaaac ctgattcagg ggaaattaaa attggagaaa cagtcaaggt
     5041 tgcctatgtc gatcaatcac gcgatgttct ggaaagtgat aaaactgttt ttgaagcaat
     5101 atcaggtggt aatgatattt tagtggttgg taaatacgaa acgccatcaa gagcttatat
     5161 tggtcgattc aattttaaag gcccagatca acagaaaatt gtaggtcagc tatcaggtgg
     5221 agagagaggt cggttacatt tggctaaaac gctgatatcg ggggggaatg ttttattact
     5281 ggatgaacct tcaaatgacc tggatgtgga aacactccgt gcgcttgaga atgcattgct
     5341 agaatttgca gggtgtgtat tggttatctc tcatgatcgg tggtttcttg atcgaattgc
     5401 gacccatatt ctagcgttcg aaggagattc gcaagttact ttttttgcag gaaattacca
     5461 agaatatgaa tccgacaaac gtcagcgact tggcggagaa ggtgccaaac ctaaacggat
     5521 aaggtataaa cctatcgctc ggtaattact tggaatggtg attgatcaga ttaactcaag
     5581 tattatttga ctattacaag gttgaagtac tcaaccttgt ttttatgcgc gaaggaacaa
     5641 ttcaggaaat gtgggaatgg gtaaatggtt agtactactt atcgtgattt ggtttatttt
     5701 ctgggtgctg aaaagtctcc agagattaaa agctaaaacc caccacaatt caattgatgg
     5761 gattgaagac atggtgcaat gtcgctattg cgaaatccat atccctaaaa aagaagccat
     5821 agtagctgat aaccatttct actgctgctc tgagcactat cagctacatt cccagtcaga
     5881 tacttaatca tatggatgat aatttacgat taacccagag cgaagcagta aataacacca
     5941 tggctcctgc ttgatgagtg gcagcaagcg ttaagggtac tgctgataat agcgtcgcaa
     6001 cccctaagct aatctgtaaa attaaagcca gtagcaacaa atggcatgcc atttttgcgg
     6061 atggagaaag tgagtattta atacatcgca accagaaaaa tgggacgaag aaggcaagta
     6121 gccaagcaat caaccggtga tcaaattgaa cggtcgttat gttttcaaaa aaattgcggt
     6181 accagggctc tagtacaaac aagcctggcg gaataagatg gccatccatt aaagggaaag
     6241 tattataggc ttttccagcg tggatacctg caacaaagcc gcctgataaa atcatgataa
     6301 aaatcagcag tgtcagataa aaagaataac gacgtaagtg ggaataataa ctttctgttt
     6361 caggctgttg ttgtttctta gcaggtgata gcaaacctgt cgctacccag aacattgccg
     6421 cataaatgat aaacgctaat ccaagatgtg ctgttaatcg atattgactg acatagatat
     6481 tgttaacgag accgctcata accatatacc aacccatcaa gccttgtagc ccaccaagta
     6541 cgaaaatacc caggagcttt aaaccgaggg ggcgatcgat ttgcttgcgt atcaaaaaat
     6601 aaagaaaggg aatgaagaat acgagcccga tagcacgtcc tagcaagcga tgaaaatatt
     6661 cccaccagaa aatccctttg aattcttcta gcgtcatgcc tttgtttact tcatgatatt
     6721 gcggtatctg acgatatttt tctaacaaaa tttcccaatc agcttcgctg agtggtggaa
     6781 tcgctccaat tagcggctgc cattccacaa tggataaacc tgaaccggtt agtcgggtta
     6841 ctccgcctac aacaaccatt gcaaagacaa gggcacaaca gataaatagc cagatggcga
     6901 taggtttttg cataattctt taaaagttaa tgagttttta atcaaataat tatcgggccc
     6961 gtaacaatcg ttgtttggta cggtcccatt ctttttgttt ttctgattca cgtttatcgt
     7021 attgtttttt tcctttggct aaaccaattt ccaactttat tctgcccgtc ttataatgca
     7081 tatcaagtgg aacgagggtg taaccagctc gttcgacttt gccaattaat cgtttaattt
     7141 gttctgcgtg taataataat ttgcgcgttc ttaatggatc aggattaata tgcgttgatg
     7201 cagtaggtaa agcgctaata tgacttccaa tcaaatataa ttcattattt cgaataatga
     7261 cgtaggcttc ctttaattga acacgtccag cacgaatgga tttgacttcc caaccttcaa
     7321 gaacaatacc tacttcatat ttttcttcga taaaatagtc atgaaaagct tttttatttt
     7381 gtgcgatact catcgggaat tggcaagaat aaaggaaagc agcttaaaat tgataagttt
     7441 atcagttttc cagtcttaac tcagttgaaa acaaaggatc attgaattgt atggcagaaa
     7501 tcaaaaaagt tgtgtatgtt gattactcag caggtcagat gtttagatta gtggatactg
     7561 ttgagaatta tccagaattt ttgccttggt gcgatggtac gtcagtaaaa cagctaagtg
     7621 ataccattgt tcatgccacg gtgaatatta attatgggcc tgttagacat agttttacta
     7681 cagagaatac gcgtcaggtt cctgaattga ttaaaatgaa attactggat gggccttttg
     7741 aagaactcga cggggaatgg cgatttattt cgttgtcaga gcatgagtgt aaaattgaat
     7801 ttaatctaca ctatactttt tctaatcgat tactcgagaa gttagtaggg cctgtttttt
     7861 ttggaatagc aaatagcttt gttgatgctt ttattaagcg agccgaaaaa atttatggca
     7921 tttctcagtg aaaaaaatat cgaggtcgag attgcttatg cgctttcgcg acatcagttt
     7981 cttcaacgaa taaccgtgcc tagtggtatc acggttgagc aggcgattac attatcagaa
     8041 tttgataaac gctttcctga tatcgactgg gtttcacaga aaataggtat ttttggaaaa
     8101 ttaactactt tagatgctgt gttgcaaaac aatgatcgcg tcgagattta tcgacccttg
     8161 cagtgtgacc caaaagaaaa tagaagaatt cgatataaaa agaaaacagt tcgcttatct
     8221 ggcatttaat tataaatttc tatcataaag tgaaggatgg ttgaaggtgt tatacgttat
     8281 caatggggaa gatgttccga atagttctga gaaacgtttg attgcgagac ctggtcattt
     8341 ggaacgcctc caagtattgc agcaagaggg taggctggtt ttagcggggc cttatccagc
     8401 aattgataat gttgatccgg gtccagccgg gttttcagga agtttaattg ttgcggaatt
     8461 tgaatcattg gaagtagccc aggcatgggc taatgctgat ccttatgtat tagctggcgt
     8521 gtatctgcat gtgacagtta aaccatttaa aaaagttttt ccaacgtgac aaagataaaa
     8581 ttaatcgttt tggccatgaa ggcctaaaat tttattaagg ctcggcttat aatatgtaaa
     8641 atccatggcg tgtgatggag ttgagagtag cgatttgttt tcaattacta aaatagagga
     8701 agttgcatgt acctgagaaa atttttgttg ttaatgttag ttatgatcac tggtttggtt
     8761 ataacctctt ctctttttgc acaaactgtg gcaaaagtga atggggttgg gattccccaa
     8821 gcgcgcctag attttatggt taaagcagcg attgcccaag ggcagcaaga ctcaactgaa
     8881 ttaagaaact cacttcgtga gaatctcatt gcagaagaga tcgttgcaca agaagcgata
     8941 aaaagcgggc ttgataaaag ttcagatgtg atgacccaaa tcgaacttgc gcgacaagca
     9001 atacttgtta gggcatatca agcggagtat ataaaaaata atgcggtagg ggatgacgtt
     9061 ctacgtcgcg aatatgagga agtcaagtcg cagatgggag acaaggaata taaagcacgg
     9121 catatcctag tagaaactga aaaagaagca aaagatatta ttgctgcgct caagaaagga
     9181 ggggcttttg ataagctcgc taaggagaaa tcaattgata ccggaagtaa aagtaatggc
     9241 ggtgaattag gttggagttc tgctggagcc tatgttaagc catttgctga tgctcttcaa
     9301 aatttgaaga agggtaatac gacagatcaa cctgtacaaa catcatttgg ttggcacgtt
     9361 gttcgactgg aagatgttcg tcctgctgtc gctccaccat tcgatgaagt gaaaatgaat
     9421 atgcagcagc gtgttttaca acgtaatttt gcttcaacta ttcaaaattt gcgcaatagt
     9481 gcgaaagtag aataaaagcc agaaaaagcc agtgagtcaa caaaatcttg ctggcttctc
     9541 aaaattactc agtggtgatt acttttaccc tcgaagaaag tgaacaaaat agctcatagc
     9601 ttattgtatt cgctgattta gcaacttctt cgatgggtaa atggttaccc cataatatga
     9661 ctgggctgcc gacagtgacg ttcgtgatgc ctgtcaaatc aactgtaagc atatccatcg
     9721 atatccggcc cacaatacga gtacgctgac catttacgat gataggcgta cctgtttgag
     9781 catggcgcgg atagccatca gcatagccag cagcaacgat tccaatttgc atcggttgtt
     9841 gggctcggaa agtaccacca tatccaactt tatcatttgt attgagttgt tgcagtgcaa
     9901 tgattttgct tgaaacggtc attacaggct ttagatcaag ttcagtggct gttttatgga
     9961 ttaaagggga tgcaccatag agcatgatgc cagggcgtac ccagtcctga tgggtttggg
    10021 gatgctggat aattgcggca gagttagcta aggagcaagg taaccgagta atattaagta
    10081 tttctgaaaa aatatttaat tggtcgttga tttcacgatc ggctgcaggg tcatccgcac
    10141 aagccaagtg cgtcatcaag gttatttcac caacgtgcga taaattcttt aaggtatgga
    10201 tcgctttagt gccttctcga ggagcgagtc ctaatcgatt catcccagta tttattttaa
    10261 ggaatagatt tatttttttg ttagctcgat gctttgctaa catggctatt tgctcagggt
    10321 gatgaatgac ggtatctaat tggtaatgat caattgatat aaggtctgcc tcagaaaaaa
    10381 aaccttctag caacaatata ggttgatgaa ttccgatttc tctcagttga attgctgcct
    10441 ctaattcaag taaagcaaaa gcatcgattt cgtgaagtgt tttagctacg cgtattaatc
    10501 cgtgaccata agcgttggct tttataacag ccatgattcg tgaatgtttt gcgtattgac
    10561 ggacgatgga aagattgtgt cttaacgcgg ctaaatttat ttgagcgctg attgggcgtg
    10621 acattcgaga gttgaattgt ttgtgatgtc gcaatgatta gtcaattttt aactgtttgt
    10681 atcgcattga acgtgacgct tattatttac acatacccaa aaatagggaa taggcataga
    10741 attagctttt aatcataatt aaaatcgttg aagcctatca gaatgttcag tttttttaaa
    10801 tcccagaaaa atcctccaga agatcaatca cagtcagtaa gctttgttag caagattaag
    10861 caagggttag cgcgaactag agaaaatcta ggtaagcaat tatcgagctt atttggagct
    10921 aggaaaatcg atgaagccgt ctatgaagag ctcgaaacaa ttttacttac ggctgatgtg
    10981 gggatacagg cgaccaatca gttgttagac tctttgcggg gcaaggttaa gcgtgatcaa
    11041 ttaagtgata ctcatcaatt gaagggagtt ttacaagaaa cactcattga attgcttcaa
    11101 ccgctagcga gaccccttga tatatcgact catcaacctt ttgtaatcat gattgcaggg
    11161 gtaaatggtg ttgggaagac gacaacaatt gggaagctcg cttattattt ccagtctcaa
    11221 ggaaaatcag ttctattagc agcaggtgat actttccgcg ccgctgcacg agagcagttg
    11281 gtcgaatggg gtaagcgtaa taatgtgaca gtgatcgcgc aagaaagcga ttctcccggg
    11341 aaaagtgatc ctgcagctgt tatttttgat gcaatcaatg cggcaaaggc aagaaatatc
    11401 g
//
LOCUS       DAOQLJ010000069        11373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2208_length_11373_cov_6.610709, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000069 DAOQLJ010000000
VERSION     DAOQLJ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 11373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11373
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2208_length_11373_cov_6.610709"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..2903)
                     /gene="prsT"
                     /locus_tag="PK522_06285"
     CDS             complement(99..2903)
                     /gene="prsT"
                     /locus_tag="PK522_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HNC41377.1"
                     /translation="MAITNYYKGTTKKKFTTVLVLILLILTTITACGKAKDADALISE
                     AKQYHEKGDDKAAIIQLKNALQQKPDDREARHLLGAIYNRTGDVRSAEKELSRALSLG
                     MDPARVLPDLGQALLSLGEFQQVLDKTETLPEDVDFAEILTLRGNALLGLGRFQEAKE
                     LFDQALQDKPDFPDAFIGLARHALSQKDVETAMRFLEEVTTKFPDNTNALIFKGDLLR
                     AQGKNDLALDVYGQAIKARPDQVTGYVNRASVLIGLGKFDSAQQDLNTASKIAPGNLL
                     IAFNQALLDFTQGKHAVALDALQKILSSAPNHMPSVLIAAASHFALGSVLQAEQLVNQ
                     YLKVIPNNLYAIKLKASILLKNHAAKQAIDLLTPALKSAQQDPQLLALAGESYMQSKD
                     FDKATEFFEKANALLPESAALHTALALSKLGQGNRESAIAELEAAANLDEQSPRAGVL
                     LVMTHLQTKDFSKALKAAEELEKEQVDSPLVQNLKGSIYVGMQDLAKARASFEKALAL
                     QQDYFPAISNLARLDVQENNLERAKNRFEEVLKRDKKHIQAMHALAGIALTQNNKNEA
                     ITWFERASNENPNELQPALQLGALYVRLQEGNKALSLAKKLQGSYPDNLSVVELLAQA
                     QLAVNDRVAALENYQKLAARLPDSAAAQFQLANVQALLQNQKAAAEAVKKAIALKPDY
                     LEANLLQARLEIQNNKPDEAMKIAKAIQKQHAKLPVGFELEGDLLMSQKKAAQAIGAY
                     EKAFSLNQSSQLMIKQYAALSQGGKGKQANERITKWLEQHPTDASTRLFLGGVHLEKK
                     QFDAAIKQFQMASEQNPDNPIILNNLAWTYQQIKDPSALDYAEKAYKNAPESPAILDT
                     LGWILVEKGEIARAVSLLQKAVSLAPEAAEIRYHLAVSLQKSGDKGAARQELEHLLAS
                     GKAFSKIDDAKKLLKDLEKD"
     gene            3190..4470
                     /locus_tag="PK522_06290"
     CDS             3190..4470
                     /locus_tag="PK522_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNC41378.1"
                     /translation="MDTVAVVGLGYVGLPLAVEFGKKRNTIGFDLSTSKIESYKRYID
                     PTDEVSTADLKAATQLTVTADPTELSKADYIIVAVPTPVDQAHQPDFSALIGASEIVG
                     RNMKRGAIVIYESTVYPGATEEVCIPAIEKYSSLQWKKDFFVGYSPERINPGDKQHTL
                     TTITKVVSGDSPETSEKVAHLYESVITAGVYRASSIKVAEAAKVIENTQRDLNIALMN
                     ELAIIFDKLGIDTLEILQAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAEMVGYIPQ
                     VILAGRRINDNMAKFIAEQTIKQIIKAGFNVKGAKVIVLGLTFKENCPDLRNSKVADV
                     ISELKDYGVDVYVHDPIAEAKEAEHEYGVTLQSWENLPKAHAIVAAVSHRDFIQKPLA
                     EICLKLVDKGCFIDVKSQFNESELCKAGLSVWRL"
     gene            4486..5514
                     /locus_tag="PK522_06295"
     CDS             4486..5514
                     /locus_tag="PK522_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC41379.1"
                     /translation="MTRYQIIQQTLSTRPYRWLVTGVAGFIGSNLLEALLKLNQKVVG
                     LDNFSTGYQHNLDQVKAAVSPTIWANFTFHHGDIRSLQDCQKACESIDFVLHHAALGS
                     VPRSIEDPIQTNESNISGFINMLVAARDANVKRFVYAASSSTYGDHPDLPKIESVIGR
                     PLSPYAVTKYVNELYADVFARCYGVQSIGLRYFNIFGPRQDPNGAYAAVIPQWIAALI
                     QNKTLKINGDGETSRDFCFVENVIQANLLSALAEESAAINQVYNIALNERTSLNQLYS
                     MMRKLLINKFPHLQNHQPIYVDFRQGDVRHSQADISKAMNLLGYAPTHRIDKGLEEAM
                     DWYIENLS"
     gene            complement(5547..5825)
                     /locus_tag="PK522_06300"
     CDS             complement(5547..5825)
                     /locus_tag="PK522_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41380.1"
                     /translation="MTLKSTITEFNTYLGDKESILDKNYQRIAEQIKLLWGYDEFFVF
                     IEKLVLVEKERNRGGFPYEVILELNILQDIHRKKFSKRSVDQKIRFHS"
     gene            complement(6118..7263)
                     /gene="ftsZ"
                     /locus_tag="PK522_06305"
     CDS             complement(6118..7263)
                     /gene="ftsZ"
                     /locus_tag="PK522_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNC41381.1"
                     /translation="MYEIMNSEGQEAVIKVVGVGGCGGNAVDHMIQNATKGVEFICLN
                     TDAQALKGNRAPVLLQLGSGITRGLGAGANPDIGREAALEDRDRIAELIQGADMLFIT
                     AGMGGGTGTGAAPVVAQIAKEMGILTVAVVSKPFSFEGKRLKTAQAGMEALSQHVDSL
                     IVIPNDKLMMVLGNDISMLDAFKAANDVLYGAVAGIAEVINCPGLVNVDFADVKTVMS
                     EMGMAMMGSAIASGVDRARMAAEQAVASPLLEEITLSGARGVLVNITASSGMKMREVH
                     EVMNTIKELTAEDATVIVGTVIDEAMQDTLRVTLVATGLGSVTQSQSRPLTVIHTRTG
                     TDDRALSPFDEPAVMRTGRRNNATVAAMQQSGVDPLDIPAFLRRQAD"
     gene            complement(7338..8573)
                     /gene="ftsA"
                     /locus_tag="PK522_06310"
     CDS             complement(7338..8573)
                     /gene="ftsA"
                     /locus_tag="PK522_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNC41382.1"
                     /translation="MNKPKVERDLIVGLDIGTSKIVAIVAEIRPEGGFEIVGLGSYPS
                     RGLKKGVVVNIETTVSAIQRALEEVELMADCKISEVYTGIAGSHIRGFNSHGMVAIKD
                     KEVTAFDVEKVMETAKAVNIPADQQILHILNQEFIVDGQEDVREPIGMSGIRLEVKVH
                     IVTGAVSAAQNIIKCVHRCGLEVRDLILQPLASAIAVLSDDEKDLGVCLVDIGGGTTD
                     IAVFTNGAIRHTSVIPIAGDQITNDIAMALRTPTKDAEDIKCRYGCALRGLADVREMI
                     EVPDVGNRGARQLSKQTLAEVIEPRVEELYLLIQAELRRSGFEQLLSSGIVITGGTSS
                     LQGMVELGEEIFHMPVRLGLPKYSGGLEEVVKTPRYSTGIGLIMAGIDEQRHHHNHLK
                     GGSAKQVFARMKGWFQKNF"
     gene            complement(8592..9374)
                     /locus_tag="PK522_06315"
     CDS             complement(8592..9374)
                     /locus_tag="PK522_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HNC41383.1"
                     /translation="MWNNHKALNLLANLILTGVVLSVAYFLVSSMVKLPLFDLKEIRI
                     ESVSNTDQSDKLALQHINRQQIQRVVGDAVIGNFFTVDLNRVKNAFQELPWVRVAKVQ
                     RNWPLGIKVILEEHSALAYWGDTALVNLQGEIFRAPSDKALPVFRGPTEESAALIAEQ
                     YKIMNRTLSPINQHVAEISLSPRYAWFIRLDNGTLLKLGRKKIKSRLERYISVYKNNI
                     VNLNQSIPLDYVDLRYPSGFAVRMDEVIPASTSPKPISRRKS"
     gene            complement(9364..10281)
                     /locus_tag="PK522_06320"
     CDS             complement(9364..10281)
                     /locus_tag="PK522_06320"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNC41384.1"
                     /translation="MNTVNSGKVAVLLGGRSAEREVSLRSGEAVLNALIRQGIDAHPF
                     DPSEQSLEELVRQGFDRAFIALHGRFGEDGSIQGALELLGIPYTGSGVLASALAMDKW
                     RTKLIWQAVGISTPRFLILDGQKAAEEVIDTLGLPLIIKPAREGSTIGLSKIKTPQAF
                     NEAYQLALNHDSIVLAEQFIEGKELTAAILGSKPLPLVRIEVSGDLYDYQAKYLSNDT
                     RYYCPSGLPDDLTEQIQRQALQAHTILGCYGWSRVDVILDHDHRPFFLEANTSPGMTD
                     HSLVPMAARAAGISFDQLVVQILALTHVE"
     gene            complement(10278..11273)
                     /gene="murB"
                     /locus_tag="PK522_06325"
     CDS             complement(10278..11273)
                     /gene="murB"
                     /locus_tag="PK522_06325"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNC41385.1"
                     /translation="MQSSAAVMDLFDFDNSIEQDIVDLGQLRGQLKINEPMGKHVSWR
                     AGGTARYFYIPQDRNDLSRFLSLLPEKIPLYMIGLGSNLLVRDGGFDGVIVGLHARLN
                     DLFISHMDGSGGSVFAGAGVSCAKLARFMGMSNLVGAEFLAGIPGTVGGALAMNAGCY
                     GGETWNIVERVQVIERSGNIIEKNIDDYQIGYRSVIRKSNDNKQGDEEWFAGAWFKLA
                     RGDGSVSKQKIKELLTARIAAQPLNYPNAGSVFRNPAGDYAARLIEFSNLKGLQIGGA
                     MISTKHANFIINTGNATAADIETIITEVRHRVREQTGVSLTQEVRIIGNTKVITQ"
BASE COUNT         3263 a         2661 c         2047 g         3402 t
ORIGIN      
        1 ctattgatct tgctatataa gatgaaaaac actgagaata gttcataggc attattcgtt
       61 aagttatccg tagccacctt atgctcagtt aggggtaatt agtctttttc taaatctttt
      121 aataattttt tagcatcgtc aattttggag aacgctttcc ctgaagctaa taagtgctct
      181 aactcttggc gtgctgcacc tttatcacca gatttttgta gacttactgc aaggtgatag
      241 cgtatttctg ctgcctcagg cgcaagtgaa actgctttct gtaaaagaga gactgcgcgt
      301 gctatttctc ctttttctac gagaatccaa cctaatgtat cgagaatcgc aggactctca
      361 ggtgcgtttt tataggcttt ctccgcatag tctaatgcag aaggatcttt gatctgctga
      421 taagtccaag caagattatt gaggataatt gggttatcag gattttgttc cgatgccatt
      481 tggaattgtt taatcgccgc atcaaactgt ttcttttcta aatgaactcc gcctaagaat
      541 aaacgcgtgg aagcatcagt aggatgttgt tcaagccact tcgtgatacg ttcattcgct
      601 tgttttcctt ttccaccttg actcaacgct gcatattgct taatcatcaa ttgactcgat
      661 tgattgagcg aaaaagcttt ttcatatgcg ccgattgctt gcgctgcttt cttctgactc
      721 atcaacagat cgccttctaa ttcaaagcca acaggtagtt ttgcatgttg tttctggatc
      781 gccttagcaa ttttcatcgc ttcatcaggc ttgttattct gaatttcaag ccgagcttgt
      841 agtagatttg cttccagata atcgggcttc aacgcaattg cttttttgac tgcttcagca
      901 gcagctttct ggttttgtag caatgcttgc acattcgcaa gttgaaattg tgcagctgct
      961 gaatctggta atctagctgc tagtttttga tagttttcaa gcgcggctac cctatcatta
     1021 actgcaagtt gagcttgcgc aagcaattcc actacgctca agttatcggg gtaactccct
     1081 tgaagttttt ttgcaagact caaagcttta tttccctcct gcaaccgcac ataaagcgct
     1141 cctaattgca atgcgggctg gagttcattg gggttttcgt tacttgcacg ctcaaaccat
     1201 gtgatcgctt cattcttgtt attttgcgtc agcgcaattc ctgctagggc atgcatggct
     1261 tgaatatgtt ttttatctcg ctttagcact tcttcaaaac gattcttagc gcgctctaga
     1321 ttattttctt gtacatccaa tctagcaagg ttagaaatag caggaaaata atcttgttgc
     1381 aatgcaagtg ctttctcaaa gctagcgcgt gcttttgcta aatcttgcat tcccacatag
     1441 atactccctt taagattctg aaccaaagga ctatctactt gctccttctc aagttcttct
     1501 gctgctttta aagctttgct gaaatctttc gtctgcaaat gagtcataac gagtaataca
     1561 cctgcacgcg gtgattgctc atccaaattc gcagccgctt ctagttcagc aatcgcactt
     1621 tcacgattac cttggcctag cttactcaaa gcaagcgcag tatgaagtgc agcactttct
     1681 ggaagcaacg cattcgcttt ttcaaaaaac tcagttgctt tatcaaaatc tttactttgc
     1741 atataacttt caccagccaa tgctaatagc tgaggatctt gctgcgctga tttgagtgca
     1801 ggtgtcaata agtcgatagc ttgctttgct gcatgattct ttagcaagat agacgctttt
     1861 agtttaattg cataaagatt attaggaatt acttttagat attgattaac gagttgttca
     1921 gcttgtaaca ctgatcctag ggcaaagtgg gaagccgcag caatcagcac gctcggcata
     1981 tggttcgggg ctgaacttaa aatcttttgt aaggcatcga gtgcaactgc atgttttcct
     2041 tgggtgaaat cgagcagcgc ttgattaaat gcgatcagta aatttcccgg tgctattttg
     2101 ctagctgtat tcaaatcttg ctgagcggaa tcaaattttc ctaaaccgat taaaaccgag
     2161 gcacggttaa catacccagt aacctgatca ggcctagctt tgattgcctg accatatacg
     2221 tcgagcgcca aatcattttt accttgtgcc cgtaacagat cgcccttaaa aattaaggca
     2281 tttgtgttat ccggaaattt tgtcgtcacc tcttctagaa aacgcatcgc agtttcgaca
     2341 tctttttgtg agagcgcatg tcgagcaagc ccaataaatg catccggaaa gtctggcttg
     2401 tcttgaagtg cttgatcaaa taactcttta gcttcttgaa atctgccaag tcccaataaa
     2461 gcattacctc taagtgtcag aatctcagca aaatcaacat cttctggcag tgtctcagtt
     2521 ttatctaata cttgctgaaa ctcgcctaaa ctgaggagcg cctgccccaa atcaggaagc
     2581 actctcgcag gatccatgcc taaactaagt gcacgactca attctttctc tgctgagcga
     2641 acatcaccag tccgattgta aatagcgcct aaaaggtggc gtgcttcacg atcatctggc
     2701 ttttgctgta atgcattctt gagctggata atagcggcct tatcatcccc tttttcatga
     2761 tattgttttg cttctgaaat aagcgcgtct gcatctttgg ctttaccaca agcagtaatc
     2821 gttgtaagta tcaataaaat taaaacaaga actgttgtaa attttttttt cgtggtacct
     2881 ttataataat tagttattgc cattacttaa tctccctaat caataaatta gacagccaat
     2941 tcgatagaaa aacccattct aatcagttaa ttcaattccc aagatcagtt ttctcattag
     3001 tcgagtctat accttttttg aatttatacc ataagtatat gatatagatt ttctaaaata
     3061 gcgcatcttt gttgtgttgt tgcacgagct aacttggatg attccatgta atctatagca
     3121 caaatgagaa actaataggg ttaagaaaca aaagcttttt gaataattag cgaataaaag
     3181 gaaatgttta tggatacagt agctgttgta ggattgggat acgttggatt accgttagcc
     3241 gttgagtttg gaaaaaaacg taataccatt ggttttgatc tttcaaccag taaaattgaa
     3301 agctataagc gttatatcga cccaacagat gaggtatcta ccgctgatct aaaagcagca
     3361 acacaattaa ctgttacggc cgatccaact gaattatcta aagcagatta tattatcgta
     3421 gctgttccga ccccagttga tcaggctcat caacctgatt tttctgcact gatcggtgcg
     3481 agtgaaatag taggtcgcaa tatgaagcgc ggcgcaatcg tcatttatga atcaacggtt
     3541 taccctggtg caaccgaaga agtttgcatt cccgcaatag aaaaatattc tagcttgcaa
     3601 tggaaaaaag atttttttgt aggatattca ccggaacgca ttaatccagg ggataagcaa
     3661 cataccctta caaccattac caaggttgtg tctggagaca gcccagaaac gtcagaaaaa
     3721 gtagcgcacc tctatgaatc agtgataact gccggcgtct atcgagcttc aagtatcaaa
     3781 gttgctgaag ctgcgaaagt tatcgaaaat acccaacgtg atttaaatat tgcgctcatg
     3841 aatgagctcg caatcatttt tgacaaattg ggaattgata cattagaaat cctccaggct
     3901 gcaggaacca aatggaactt tttacctttc agacctggcc tggttggagg acattgtatt
     3961 ggggtcgatc catattatct tacccataaa gccgagatgg tcggctatat tccgcaagtt
     4021 attttggcgg gacgacgcat taacgacaat atggctaaat tcatcgcaga gcaaacgatc
     4081 aaacaaatta ttaaagctgg ctttaatgtt aaaggcgcta aagtaatcgt gttggggtta
     4141 actttcaagg aaaattgccc tgatttacga aattctaaag tagcagacgt catatcagag
     4201 cttaaagatt atggagttga tgtctatgtt catgatccca tagctgaggc caaagaggct
     4261 gagcacgaat atggtgtaac cctgcaatct tgggaaaatc tccccaaagc ccatgcgata
     4321 gtagctgctg tctcgcatcg agatttcatt cagaaaccat tagccgaaat ttgcttaaaa
     4381 cttgttgata aaggatgctt tattgacgtc aaatcacagt ttaacgaaag cgaattatgt
     4441 aaagcaggat taagtgtctg gcgtctataa ctcggtattc ccaatatgac tcggtatcaa
     4501 attattcagc aaacactgag caccaggccc tatcgctggt tagtcactgg cgtagctggt
     4561 tttattggat ctaatttact agaagcgtta ttaaagctta accaaaaagt agttggtctc
     4621 gataattttt caaccggcta ccagcataac ctcgatcaag tcaaagccgc ggttagtcca
     4681 acaatctggg ctaattttac gtttcatcac ggtgatattc gatcactgca agactgccaa
     4741 aaagcttgcg agagtatcga ttttgtattg catcatgccg cattaggctc agtaccacgt
     4801 tcaattgaag atcctatcca aaccaacgaa agcaatatct caggttttat aaacatgtta
     4861 gttgctgctc gtgacgcaaa cgtcaaacgg tttgtctatg ccgcttctag ttctacctat
     4921 ggagatcatc ccgatttgcc aaaaattgaa tctgtaatcg ggcgcccgct gtctccctat
     4981 gctgtcacca aatatgtcaa tgaactttac gctgatgttt ttgcacgatg ctatggtgtc
     5041 caatccattg gtctgcgcta cttcaatata tttggtcctc gacaagatcc taatggcgcc
     5101 tatgcagcgg taattccaca gtggattgct gcgttgattc agaacaaaac acttaaaatt
     5161 aacggggatg gcgaaacaag ccgtgatttc tgctttgtcg aaaatgtaat ccaagccaac
     5221 cttctctcag cattggctga agaatcggca gccattaatc aggtctataa cattgcgtta
     5281 aatgagcgca caagcttgaa tcagctttat tcaatgatgc gtaagttatt gattaacaaa
     5341 tttccccatc tacaaaatca tcagccgatt tatgttgatt ttcgccaggg tgatgtacga
     5401 cattcgcagg cagatatatc aaaagctatg aacttactgg gctatgcacc cacacatcga
     5461 attgataaag gtttggaaga agcgatggat tggtatatcg aaaatctttc ctaattctat
     5521 tgatcaataa gctaaacaga tcaaatttat gaatgaaatc ttatcttctg atcaacggat
     5581 cgcttagaaa atttcttccg atgtatatcc tgtagaatat ttagttctaa tataacttca
     5641 taaggaaatc ctcctctatt ccgttctttc tcaacaagca ctaatttttc aatgaacaca
     5701 aaaaattcat catatcccca aagtaatttt atctgctccg cgattctctg ataattctta
     5761 tcaagaattg attctttatc acctaaataa gtattaaatt cagtgatcgt actttttagt
     5821 gtcatttacc actatctcct tccatttttt ctcaattcgc ttctaatctt tatttaaaaa
     5881 taggcctatt gatgaaaaaa acaatgtatc tttgaaaata tcggcaaata caactacaaa
     5941 gttaaaatgc tgcgtaattt agttgaatag gactgagcat ttactaatct aacttaagat
     6001 tacttaagtt cataacctta tcaaggtgtt agtactaccc agtcctaatt aaattacaat
     6061 aacatgcgtc taactaaaga atctccccat gcaaaggtct aattctatcg ttcaacttta
     6121 gtcggcttgt cgtcttaaga aagcaggaat atccagtgga tcgacccctg attgctgcat
     6181 agcagccaca gtagcattat ttcttcttcc cgtccgcatt acggccggtt catcaaacgg
     6241 tgaaagtgct ctatcatcgg taccggttct tgtatgaata accgttagag gtcttgactg
     6301 tgattgagta acgctaccca atcctgtcgc aaccagtgtc acgcgaagtg tatcttgcat
     6361 cgcttcatcg ataacggtac ctacgataac ggtagcatct tctgcagtaa gttctttaat
     6421 ggtattcatg acttcatgaa cttctcgcat tttcatacca gagcttgcag taatatttac
     6481 cagcacacca cgagcacctg ataacgtaat ttcctctaat aaaggactag caacggcttg
     6541 ttcagctgcc atacgcgcac gatctacccc acttgcaatc gcagagccca tcatagccat
     6601 tcccatttct gacattacgg ttttaacatc agcaaaatcc acattaacaa gaccagggca
     6661 attaattact tcggcgatac ctgctacagc gccataaagc acgtcattag ccgctttgaa
     6721 ggcatcaagc atactgatat cgttaccgag taccatcatc agcttatcgt taggaatcac
     6781 aatcaatgaa tcaacatgct gcgataatgc ttccatccca gcttgagctg ttttcaagcg
     6841 cttaccttca aaagaaaaag gcttactgac aacagctaca gtaagtatac ccatctcttt
     6901 tgcaatttgc gctacgactg gcgctgcacc agtgccagtc ccacctccca ttcctgcggt
     6961 aataaacaac atatcagcgc cctggataag ttctgctatg cggtcacggt cctcaagcgc
     7021 tgcttcgcga ccgatatcag gatttgcacc tgcccccaga cctcttgtaa ttcctgaacc
     7081 aagctgcagc aaaactggtg cacgatttcc tttaagggct tgagcatctg tattaaggca
     7141 aatgaactca acacctttag tcgcattctg aatcatgtgg tcaaccgcat tccctccgca
     7201 tcctccaaca ccaactactt taatgaccgc ttcctgacct tcagaattca tgatttcata
     7261 cataacacct ctcctttgca tggtaaattt caaataacta cgcttctaca caacgcatac
     7321 aacctaaaat tttttggtca gaaatttttc tgaaaccaac ctttcattcg tgcaaatact
     7381 tgcttagctg agccaccttt caaatgatta tggtgatgac gctgctcatc tataccagcc
     7441 atgattaaac caataccagt cgaatagcga ggagttttga ccacttcctc caaccctcct
     7501 gaatactttg gtaggcctag cctaaccggc atatgaaata tttcctcccc gagctcaacc
     7561 attccctgca atgagctcgt cccccccgta attacaattc ctgatgaaag taattgctca
     7621 aacccacttc tgcgcagctc agcttggatt aacaaataaa gctcttctac tcgcggttct
     7681 atcacctcag caagtgtttg cttagaaagc tggcgcgcac ctcgatttcc tacatcagga
     7741 acctcaatca tttcacgcac gtcagcaaga ccacggagcg cacacccata acgacatttg
     7801 atatcttcag catctttggt cggtgtccgc agtgccatcg ctatatcatt agtaatttgg
     7861 tctccggcga tcggaataac cgaagtatgt cgaattgcac cattggtgaa taccgcgata
     7921 tcagtagttc cccctccaat atcaacgaga caaaccccta aatctttttc atcatccgat
     7981 aatactgcta ttgccgaagc aaggggttgc aaaataagat cacgaacttc taacccacaa
     8041 cgatgtacac atttaataat attctgggca gctgacacag cccccgtcac aatatgtacc
     8101 ttaacctcaa gccgtatccc actcatccca atgggttctc gcacatcttc ctgtccatca
     8161 acaataaatt cttgatttag aatatgaagt atttgttgat cagctggaat attgactgct
     8221 ttggccgttt ccattacttt ctctacatcg aaagcagtca cttccttatc tttgatcgca
     8281 accatgccat gtgaattaaa accacgaata tgacttcccg cgatacccgt gtaaacttca
     8341 cttatcttac aatctgccat gagttccact tcttcaagcg cacgctgtat cgcactgact
     8401 gtcgtttcaa tattgaccac gacacccttc ttaagccccc gcgaaggata actccctaat
     8461 cctacaattt caaaaccgcc ctctggtcta atttcagcga caatagcaac gattttagag
     8521 gtaccaatat caagaccaac aatcaaatct ctctcaactt tgggcttatt catttatcaa
     8581 tccgcctttt ttcatgattt tcttcgagat attggttttg gcgaagtact agctggaatt
     8641 acttcgtcca ttcgcactgc aaaaccactt ggataacgta gatcgacata atctaaggga
     8701 atagattgat tcaaattcac aatattattt ttataaactg agatataccg ttctaagcgg
     8761 gatttgattt tcttacgccc taacttcaac aaagtaccat tatctaatcg aataaaccac
     8821 gcatatctag gagaaagcga gatctctgcc acgtgttgat taatcgggct gagcgtccta
     8881 ttcataattt tatattgttc agcaatcaac gcggcacttt cttcagttgg ccccctaaat
     8941 acaggtaaag ctttatctga cggagcgcga aatatctctc cttgaagatt aaccaaagca
     9001 gtatctcccc agtatgccaa tgcactatgc tcttctagta taactttgat tcctaatggc
     9061 caattacgct gcacctttgc tactctcacc caaggcaact cttgaaaagc attctttacc
     9121 cgattcaaat cgacagtaaa aaaattccct atcactgcat caccaacaac acgttgaatc
     9181 tgttgtcggt tgatatgttg taatgccaac ttatctgatt gatccgtatt acttacactt
     9241 tcaattctta tttctttgag atcgaatagc ggtaatttta ccatgcttga gacaaggaaa
     9301 taagcaacag ataacacaac tccagttaga atcaagttag ccagcaaatt caatgccttg
     9361 tgattattcc acatgggtaa gtgctaaaat ttgaactact aactgatcaa acgatatacc
     9421 cgcagctcgt gcagccattg gaactaaact atgatcagtc ataccaggag atgtattggc
     9481 ctcgagaaaa aagggacgat gatcgtggtc aagtattaca tcaactctac tccaaccata
     9541 acagcccaaa atagtatgtg cctgtaacgc ttggcgctgt atttgttcgg ttagatcatc
     9601 tggcaatccg ctgggacaat aatagcgcgt atcatttgac aaatatttag cctgataatc
     9661 atacagatcg ccagacacct cgattcttac aagtggcaat ggtttgcttc ctaaaattgc
     9721 agctgttaat tccttccctt cgataaattg ctcagccaat acaatagaat cgtgatttag
     9781 cgccagctgg taagcctcat taaatgcctg gggtgtttta atcttactca aaccaattgt
     9841 cgatccttct ctagcaggtt ttatgataag gggtaagccc agggtgtcaa tcacttcctc
     9901 agccgctttc tgtccatcta gaattaagaa tcgtggagta ctaataccaa ccgcttgcca
     9961 aattaattta gtccgccatt tatccattgc taaagcacta gctagtacac cacttccggt
    10021 atagggaatt cctaatagtt ctagtgcacc ttggatcgaa ccatcttcac caaaacgacc
    10081 atgaagcgca ataaatgcgc gatcgaatcc ttgacgtact aattcctcta gcgattgttc
    10141 actgggatca aatggatggg catcaatccc ctgtcggata agcgcattga gcaccgcctc
    10201 accacttcta agggaaactt ctctctctgc tgaacgcccg cccagcaata cggcaacttt
    10261 tccagaatta actgtattca ttgggttatc accttagtat taccaatgat tctgacttcc
    10321 tgagtcaaac taacaccagt ttgctctcta acgcgatgac ggacttcagt gataattgtt
    10381 tcaatatcag cagctgtggc atttcccgta ttgataatga aattagcgtg cttagttgat
    10441 atcattgcac cgccaatctg caaccccttc agattggaaa attcgatcaa acgcgccgca
    10501 taatctccgg ctggatttcg aaaaacagaa ccggcattag gataattaag cggttgagct
    10561 gcaatccgtg cagttaatag ctcctttatt ttttgcttgg aaacactacc atcacctcta
    10621 gccaatttaa accaagcacc agcaaaccac tcttcatctc cttgtttatt gtcattcgat
    10681 tttctgatca cagaacgata cccgatttga taatcatcaa tatttttttc tatgatgttt
    10741 cctgagcgct caataacttg aacgcgttca acgatattcc aggtttcacc accatagcag
    10801 ccagcattca tagcaagtgc tcctcccacc gttcccggga ttcctgctaa aaactcagcc
    10861 ccgactaaat tactcatgcc catgaatcga gccaatttag cacaagatac accggcccct
    10921 gcaaaaacac tccccccact accatccatg tggctaataa acaaatcatt caatcttgcg
    10981 tgcaatccca caattactcc atcaaaccca ccatcccgaa ccagcagatt acttcccaaa
    11041 ccaatcatat agagaggtat tttttcagga agcagcgaca agaatcgtga taaatcattg
    11101 cgatcttggg gaatataaaa atacctcgca gtaccacctg cccgccatga aacatgcttt
    11161 cccattggct cgttgatttt cagttgacct ctcagctgac ccaaatcgac aatatcctgc
    11221 tcaatcgaat tatcaaaatc aaatagatcc ataaccgccg cactagattg cattaaaacc
    11281 tcttaccaaa gtacacggaa agtttttatt cctcgattac ccaatcgcaa ccaacttttt
    11341 tgcagttttt gacacccatg cggcaacctt tcc
//
LOCUS       DAOQLJ010000070        11365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2211_length_11365_cov_6.134394, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000070 DAOQLJ010000000
VERSION     DAOQLJ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 11365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11365
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2211_length_11365_cov_6.134394"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PK522_06330"
     CDS             <1..278
                     /locus_tag="PK522_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41386.1"
                     /translation="FGNMMKAKGDFKVTSDTAIAVLLAIPGYETQGARFTPLATADSV
                     SNHEHDHSHDHDHRDDHSDNHHHDHDKKHDCEHCKAQHKEHHHHHKH"
     gene            complement(309..917)
                     /gene="cobO"
                     /locus_tag="PK522_06335"
     CDS             complement(309..917)
                     /gene="cobO"
                     /locus_tag="PK522_06335"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNC41387.1"
                     /translation="MKDLATQAERHRERMQRKKEIVDAAIERANQEKGLLLIHTGNGK
                     GKSSAAFGMIARALGHDMRVGVAQFIKGRSDTGEEAFLRHQPGVEWYVLGEGYTWDTQ
                     NLAQDIETAQRGWQLARTMLQNETINLVVFDELTYPIRYGWLNLDQILIDLENRPAMQ
                     HVIITGRNAPDTLCEIADTVTEFRNIKHAFYSGIKAQKGIDL"
     gene            complement(959..1528)
                     /locus_tag="PK522_06340"
     CDS             complement(959..1528)
                     /locus_tag="PK522_06340"
                     /inference="COORDINATES: protein motif:HMM:NF022526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2780 domain-containing protein"
                     /protein_id="HNC41388.1"
                     /translation="MNISIFRIFPLLLVMIVLMSCSSPGQLPSSSGAASVLQQSTEAI
                     QTPTAAINSVPTSDGLIGLLMQQLGVSQTQAEGGTGALLQLAKSKMSPGDFTALGNSI
                     PNMQSIMAAAPSMNTSELGVPKSLAGMTGGNMPGANSGMLGIAGAFQQLGLAPDMVQK
                     FIPVITQFVQGNGGGAVTSALQSALMGGL"
     gene            complement(1636..2541)
                     /locus_tag="PK522_06345"
     CDS             complement(1636..2541)
                     /locus_tag="PK522_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNC41389.1"
                     /translation="MNFLITGATGFVGQRLVARLKTEHCIKVLSRNRIHAERLLGVPA
                     YDWNYLLDPVPAEALEDTQVIIHLMGENLGNGRWTEQRKQEIYQSRILSTRKLVEATT
                     PNLKRFICGSAIGIYPGIGDSVYDETFVVPTNGNFMQTICRDWEDEVRKIENRGVRSV
                     SIRTGVVLGLGGMLEKLLPLFKLGLGGSIGDGKQWLPWIHIDDLVSVFIAAALDDHYQ
                     GPVNAVSPNPVQYQEFSETLGKVLHRPTFLTVPAFILKLALGEAAALALNSYRIEPAK
                     LIESYDFHFKFTQLGTALENIIQKS"
     gene            2666..5110
                     /locus_tag="PK522_06350"
     CDS             2666..5110
                     /locus_tag="PK522_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC41390.1"
                     /translation="MNELTPQALRLTIDPNSLGFLDTSELIDQPLSWIGQERAEKAAY
                     FGLEMDHPDYNLFVLGEVGSGRSSLLKQAMTKVAQHKVVPADLCYIHNFEMPERPFVL
                     RVFAGKGRLLRQKLVQFVKYLQSEVPRYFEGQDFKAESERIERKFKLDEAQAYTKLDT
                     FAEQHHFAIQRESNRMIFTLLDNSGKVLTEAEVLALSKEKRQEFERLEQALQAEIASY
                     FDKVRPLERAKEEVLNDLQRSGVKRLLADELAMIRDGACISDEDQQKLNQFVTKMEED
                     ILDHLELFRISDAEEEKRLEELKSLLFCYQINLVTDNHELKGAPVVIDESPSYRSLFG
                     SIDYQSIEGAFTTDFTRIRAGSLLKAHGGFLMLYLDDLLVDNLVWEKLCRLLRSSKLQ
                     IEEPGVNLAQVATASLVPEAIDVQVKIILIGSREQYYLIQEENPELARRFRVKVDFAD
                     SFISNEQIRHAASIFVAHACQESNLPHFSAKAVARLLEDCHRDANDQLRQSAIFSRTE
                     TLVLESAAQCKVHAGCLVDVVDVETAIQARLFRHNYPDCCLQEAIADGDILIDTMGER
                     IGQINGLVQIDLGDHCFGAPVRITAHTYAGESGVLNIEREVGLSGPIHDKGVFILQSY
                     LSALFASIAPLALNASIVFEQEYSGIEGDSASCAELFALISALSGIPIKQGIAVTGAV
                     NQYGEILPIGGINEKIEGYFSVCKTAGLDGSQGVLIPQRNQHNLMLPRNVIEAVAQGL
                     FHVYTAQHIAEGVELMMGYPAGITAESNLHEIINYSHESVLGYAQRTLRAYRHACQQI
                     DRPKSEHKRFVRRTEK"
     gene            5273..5812
                     /locus_tag="PK522_06355"
     CDS             5273..5812
                     /locus_tag="PK522_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNC41391.1"
                     /translation="MRVIVLLLFVCVTQSAFAACQSGLLDHRFRKLAGTEEVNLCDTY
                     AGKVLLVVNTASKCGYTPQYDGLEKLHEQYEKRGFAVLGFPSNDFMGQEPGTEEQIQD
                     FCRLTYGVQFPMFEKVTVRSERATAFFKQLAEVSGTYPTWNFHKFLIGRDGKFIKQFS
                     PHTTPADLDMINAIEKAIQ"
     gene            5858..7831
                     /locus_tag="PK522_06360"
     CDS             5858..7831
                     /locus_tag="PK522_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HNC41392.1"
                     /translation="MRNHFPQIEVTPPLHRAGQSLSDSPSWLKSKRMLIFLIAFAVTA
                     IISMSYVLGRSSIYRSHATLLTMAKTAIDQRSVDVDLQHVAIQKQLLLGADMLAEIIK
                     TLQSHPEVKTQLTASDIRSMLDVQPVAETNLVEIVAEGPDPKILPILVNTWLDVYLKG
                     RENEIARATGDTTQRIQTEISGLTEKINQKRIELELFRENNHIVSTGREENEALSRLK
                     GLNDSLNKAIEEEVKAKARLDSIKKSIAKGQAVVPKEDTQKLAMLEKRAQELKEELAD
                     LDRRYTREYMALTPSLKVIPEKIATLQTEIDQLRSAGQRIVLSEAEQEYVAALQTTQA
                     IRDQLEAHKKKAADFTARFTEHEALKIDLEGFEQRLRETQERLIQIETKYGGKFPQVD
                     VVERAAVPLQPIWPDYSRDSLIALISSLIMGLIAVWVVEFLTRKEKAKLAINLSGVHI
                     YGGEESAREVVNQLPLTQQRMLAPQSVQPALIGGVIQEIAPQKIHLLFQAGNNKEKQL
                     MVLLLSGLTLDEITLLDPQCFDLENNQLSILGKYPRKIPLHPALRRLVEARGYNLIGA
                     SGHALNLDDLAVLLTCTIVDAGLTAAEGISADALRQTYLIYLVRQGMRLSDLAQIVGY
                     ITSTELTTLSQFAPLGPKLAASAVNLFYPLEAS"
     gene            7907..8587
                     /locus_tag="PK522_06365"
     CDS             7907..8587
                     /locus_tag="PK522_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41393.1"
                     /translation="MLRKIFTTLGFGASAMMPSEPPYTPYQSDAANEIYNLLFCDNLA
                     AFKPKEGERAATWQIVLFAEPADRSALLALANDVTQESRIRYLAYTRLRLLGENVPTK
                     VLLGVIVEVSLADGLDALAAYSDGGVRYINHSGELVFFEGSTELEPHVKKLLAASEPV
                     VARIGPWKKPRRSPPESDNVRLTFLVSDGLYFGEGPMSIMQHEEMAGPVIQSATTLLQ
                     TIVSRASE"
     gene            8755..9837
                     /locus_tag="PK522_06370"
     CDS             8755..9837
                     /locus_tag="PK522_06370"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNC41394.1"
                     /translation="MVVNKTKDRAIGAIMGAFIGDALGLGPHWYYDLNELRRDYGDWI
                     DNYTDPRPNRYHAGCKAGQLSQVGFILVLTLRSLVECGEYNKDDFCRRMDEELFPLLD
                     GTPMSGPGSYTSQSIREAWRCRVQHKLPWGQTGSHADTTEAIERTLAIAVRYAFQPNQ
                     LAAAITDNTMLTQIDHTVVSMTVAYGAVLGLLIQGHLLNTDLSGKLMELVKDGKLPFH
                     AVTRDALQPPRPGDPDPPRAGRFASPDALLTPSYMAAAYENPEIRIEPAWKVSIVYGM
                     PCAIYHQLPAAYYLAARFKDDFESAVLHAINGGGQNQARAILTGALTGAQTGLTGIPQ
                     RFLDGLDGCADLLRLAEHLAAKVGNF"
     gene            complement(10027..10392)
                     /locus_tag="PK522_06375"
     CDS             complement(10027..10392)
                     /locus_tag="PK522_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41395.1"
                     /translation="MSICLLKSSLQSGVSFKSSNSSFRELSRLPCTDGCSLSVLYRVL
                     PENDEYRNYLRYRYWSHYLAEPSSDGLTFGTWGDIPTSADVRALIWDGRTEPKQWHEV
                     SVRKLHALGPPQTDTHGVR"
     gene            complement(10898..>11365)
                     /locus_tag="PK522_06380"
     CDS             complement(10898..>11365)
                     /locus_tag="PK522_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HNC41396.1"
                     /translation="PGVLKAYGLDAGSAALKGAAAGAGIDLMVGGLSLGAASALGALA
                     GTGWSTFKRYGKEIKAKIQGTEWLCADENTLKILYLRQQQLLSKLMQRGHAAYDPDHI
                     EETVTGELPAKWSEIVSILQQNPLWQKPSPQRDQNQQFQTLQSQLINALLDNK"
BASE COUNT         3158 a         2439 c         2669 g         3099 t
ORIGIN      
        1 tatttggaaa tatgatgaaa gcgaaaggtg acttcaaggt aacgtctgat accgctattg
       61 ctgttttatt agcgattcca ggctacgaga cacaaggcgc acgatttaca ccattggcaa
      121 cagcagattc ggtgagtaat catgaacatg accactctca tgaccatgat catcgtgatg
      181 atcattcgga caatcatcat cacgatcatg ataaaaaaca tgattgtgaa cactgtaaag
      241 cgcaacataa agagcatcac catcatcaca aacattaatc atcgatttgc agttaaagat
      301 gattaatttc agagatcgat ccctttctgg gctttgatac ccgaataaaa ggcgtgttta
      361 atattacgaa attcggtaac ggtatcagca atttcacata gcgtatctgg tgcgttacga
      421 cctgtaatga tgacatgttg cattgcaggt cgattttcca aatcaattaa tatctgatca
      481 agattgagcc agccgtaacg aattgggtaa gtgagttcat cgaaaacaac gagattgatg
      541 gtttcattct gcaacatagt acgagctaac tgccatccgc gctgcgctgt ttctatatct
      601 tgagctaggt tttgggtatc ccaggtgtag ccttcgccga gcacatacca ttcaacacct
      661 ggttgatgac ggagaaaagc ttcttcaccg gtatctgatc gtcctttaat aaactgagca
      721 acaccgaccc gcatatcatg tcctaatgca cgggctatca tcccaaaggc cgcacttgat
      781 tttccttttc cgtttccagt atggatgagt agcaagcctt tttcttgatt tgcacgctcg
      841 attgctgcgt caacgatctc tttttttcgt tgcatgcgtt ctcgatgacg ctcagcttgg
      901 gtggctagat ctttcatcaa ttaatgaaca aacagattat ctggtggata aacttctact
      961 atagtccacc catcaaagcg gactgtaatg cgctggtgac cgcacctcct ccatttcctt
     1021 ggacaaattg agtaatgact ggaataaatt tctgcaccat atcgggggct aagcctaatt
     1081 gctgaaatgc accagctatt cctaacatac cgctattagc gcctggcatg ttacctcctg
     1141 tcatacccgc caaactttta ggaacaccga gttcgcttgt attcatcgaa ggcgctgctg
     1201 ccatgatact ttgcatattg ggtatcgaat tacctaacgc tgtaaaatca cctggcgaca
     1261 tttttgattt ggctaactgg agtaatgcac ctgtaccgcc ttctgcttga gtttgagaaa
     1321 caccaagctg ctgcataaga agtccgatca aaccatcgct tgtaggaaca gaatttatcg
     1381 ccgcagtagg ggtctgaatg gcttcagtgc tttgttgtag aacacttgct gccccagaag
     1441 aactaggtaa ttgcccggga cttgagcaac tcataagaac aatcattact aatagtaaag
     1501 gaaaaatacg aaaaattgat atattcatga tagttttatt actcctattt aaggataaaa
     1561 ttgaaacgaa taaaaagaat aacgatgtta atcataagac gatgcaggtt attggtaaat
     1621 caatttagct taaggttaag atttctggat aatattttct aatgcagtac caagttgcgt
     1681 gaatttaaaa tgaaaatcat aggactcaat cagtttggct ggttcgatgc gataactgtt
     1741 caatgctaaa gcagccgctt cccctaatgc taatttaagg ataaaagcgg gaactgtcag
     1801 gaaagtaggc cggtgtaaaa ctttacccag ggtttctgaa aattcttggt actgtacagg
     1861 attgggtgaa accgcattga caggcccttg gtagtggtca tcgagcgcgg ctgcgatgaa
     1921 aacggaaacc aaatcatcga tatgaatcca aggaagccat tgttttccat caccaatgga
     1981 tcccccgaga ccgagtttaa acaaagggag caatttttct agcataccac cgagacctaa
     2041 caccacgccg gtacgaatgc tgacgctacg aacccctcta ttttcaattt ttctaacttc
     2101 atcttcccag tcgcggcaaa tggtttgcat gaaattacca ttcgttggaa caacaaaggt
     2161 ttcgtcatag accgaatcac cgattcccgg ataaatccca atagctgaac cacaaatgaa
     2221 acgctttaga ttcggcgtag tcgcttcaac caatttgcgc gtacttagga tacgcgattg
     2281 atagatttct tgcttgcgtt gctcagtcca tcgaccattt cccagatttt cacccatcaa
     2341 atgaataata acttgcgtat cttcaagcgc ttcggctgga acagggtcaa gcaaatagtt
     2401 ccaatcatag gcagggacac cgagcaaacg ttcagcatgt atccgattcc tgctaagtac
     2461 cttaatgcag tgttctgttt taagccgtgc tacaagtcgt tgaccgacaa accccgtggc
     2521 ccctgtgatg agaaaattca tgttagttca ctgttctaat tgtgttcgat gcgtgatcaa
     2581 gatacccttt tctaagatag atagcaaagt gcagtaaaat tacttttttt aacagccccg
     2641 ttcaagtaaa cagattactt attgtatgaa tgaattaact cctcaagcgc ttcgcttaac
     2701 aatcgatccc aactcattag gttttttaga tacatcggag ttaattgatc aaccattgtc
     2761 atggattggg caggaacgtg cagagaaagc agcatatttt ggcttagaaa tggatcatcc
     2821 cgactataat ttatttgtgt tgggagaagt ggggagcggt cgatcttcct tgctcaaaca
     2881 agccatgacc aaagtcgctc aacataaagt cgttccagct gatttgtgtt atatccacaa
     2941 ctttgaaatg ccagaaagac cttttgtgct gcgcgttttt gcaggaaaag gacgcttact
     3001 gcgtcagaag ctagtgcagt ttgtaaaata tttacaatct gaagtaccgc gttattttga
     3061 ggggcaagat tttaaagcgg aaagtgaacg aatcgaaaga aaatttaagc tggatgaagc
     3121 acaagcctat actaagctag atacgtttgc agagcagcac cattttgcga ttcagcgtga
     3181 atccaatcga atgattttca cattgctcga caattctgga aaggttttga cagaagctga
     3241 ggtattggca ttatcgaagg aaaagcgtca agaatttgaa cgactcgagc aagcgttgca
     3301 agcagagatc gcaagttact ttgacaaagt tcgcccgctt gagcgtgcta aagaggaagt
     3361 attgaacgat ctgcagcgca gtggggttaa acggttattg gcggacgagc ttgctatgat
     3421 tcgtgatggt gcctgtataa gcgatgaaga tcagcaaaag cttaatcagt ttgtgacaaa
     3481 gatggaggaa gatatcctcg atcatcttga actattcaga atttcagatg ctgaagaaga
     3541 aaaacgcctg gaagaattaa aatcactgct attttgctac cagattaatt tagtgactga
     3601 taatcacgaa ttaaaaggag cccccgtcgt tattgatgaa agtccttctt atcgttcact
     3661 gtttggcagc atcgattatc aatcgatcga aggggcattt acaactgatt ttactcgtat
     3721 ccgtgcaggg agtttgctca aggcgcatgg cggattctta atgctctatc tggacgactt
     3781 actagtggat aatctggtat gggaaaagct ctgccgtctg ttacgcagta gtaaattaca
     3841 gatcgaagag ccaggagtaa atcttgcgca agttgcaaca gcgtccctgg tgcctgaagc
     3901 gatcgatgtt caggtcaaaa tcattctaat cggttcacgt gaacaatatt atttaatcca
     3961 ggaagaaaac ccagagctag cacgacgctt cagggtcaag gtggattttg cggatagttt
     4021 tatttcaaac gaacaaatac gccatgctgc ttcaattttt gtcgcacatg cttgtcaaga
     4081 gtccaattta ccccatttca gtgctaaagc agttgctcgc ttattggaag attgtcaccg
     4141 agatgccaat gatcagttac gacaaagcgc tatttttagt cgaactgaaa cgttggtatt
     4201 agaaagcgcc gcacaatgta aggtgcacgc gggttgtttg gtcgatgtgg tagatgttga
     4261 aacggcgatt caagcgcgat tatttcgcca taattaccct gattgctgct tacaagaagc
     4321 gatcgctgat ggagatatct taatcgatac gatgggtgaa aggattggtc aaatcaacgg
     4381 tcttgttcag attgatttgg gcgatcactg ctttggcgcg cctgtccgaa ttaccgcgca
     4441 tacttatgca ggtgaaagcg gtgtactcaa tatcgagcga gaagtagggc tttctggacc
     4501 cattcacgat aaaggcgtat ttatcctgca aagctatctg tcagccctgt ttgccagcat
     4561 cgcaccgctt gcgctcaatg catcgattgt cttcgagcaa gagtatagcg gtattgaggg
     4621 tgattctgca tcctgtgcag agctattcgc gctgatttcc gcgctttccg gaatacccat
     4681 caagcaagga attgccgtga ccggggccgt aaatcagtac ggagagattt tacccatcgg
     4741 tggcatcaac gaaaaaatcg agggttattt tagtgtatgt aagactgctg gattagatgg
     4801 atcgcagggt gtgctcattc cgcagcgtaa ccagcataac ttgatgctcc cgcgcaatgt
     4861 catcgaagca gtcgcacaag ggttgtttca tgtttatacc gcacagcaca ttgctgaagg
     4921 ggttgaatta atgatgggtt atccagcagg aattactgct gaaagcaatt tgcacgagat
     4981 cattaattat tctcacgaga gtgttctggg ttatgcacag agaacattac gcgcttatag
     5041 gcatgcgtgt cagcaaatcg atcgacctaa atccgagcat aaacgctttg tgcggcgcac
     5101 tgaaaaatag ccaacagggt tgactgtatg aattttctga tggtgattat caaatagcgc
     5161 tagcttagca attgtggagg cagtagttgt ctaacgctag caggtttatt gtgaatactt
     5221 gtatgaatgt acggttcgtc atatccttca attaacaaca aggagaattc cgatgcgagt
     5281 tattgtattg cttttgtttg tttgcgttac tcaatctgct ttcgcagctt gtcaaagtgg
     5341 attgttggat catcgtttcc ggaagctggc aggaactgag gaggtcaatc tttgtgatac
     5401 ttacgcggga aaagtgctat tggtcgtcaa taccgcgagt aaatgtgggt ataccccgca
     5461 atacgatgga ttagaaaaat tacatgagca atatgaaaaa cgtgggtttg ctgtattagg
     5521 ttttccttcg aatgatttta tgggacagga gcccggcact gaggaacaaa tccaagattt
     5581 ttgccgcctg acttatggcg tgcagtttcc tatgtttgag aaagtaacgg tcagaagcga
     5641 acgggcaact gcttttttca aacaattggc ggaagtttca gggacttatc caacctggaa
     5701 ttttcacaag tttctgattg gtcgtgatgg gaaatttatt aagcagttta gtccccatac
     5761 gacgcctgct gatcttgaca tgattaatgc cattgaaaaa gccatccagt gattgggaaa
     5821 tgattttttt cctttatgtt gataattcgg tgatcgagtg agaaatcatt ttcctcaaat
     5881 agaagttacc ccgcctttac atcgagcagg gcagtcgtta tctgattcac catcatggct
     5941 aaaatcgaag cgaatgctga tttttctcat cgctttcgct gtaaccgcaa ttatttcgat
     6001 gagctatgtg ctgggacgtt catcaatcta tcgtagccat gcaaccttgc tgaccatggc
     6061 aaaaacagca atcgaccaaa ggagtgtcga cgtcgatctt cagcatgtcg caatccaaaa
     6121 acagttgcta ctgggtgcgg atatgttggc agagatcata aaaacgctcc aatcgcatcc
     6181 ggaggttaaa acccagttaa ctgcttcgga tatccgtagc atgcttgatg tacagccagt
     6241 cgcagaaacg aatttggtcg agatcgtcgc ggaagggcct gatcccaaaa tcttaccgat
     6301 tctagtcaat acctggctcg atgtttacct taaaggcaga gaaaacgaga ttgcccgggc
     6361 aacgggcgac acaacgcaaa gaattcaaac cgagatcagc ggtttgacgg agaaaatcaa
     6421 ccagaagcgc atcgagcttg agttatttcg tgaaaacaat cacatcgttt ctactggcag
     6481 ggaagagaat gaagctttat caagactaaa agggctaaat gactcgttaa ataaagcaat
     6541 tgaagaagaa gtcaaagcca aggcgcgctt ggattcaatt aaaaaatcca ttgccaaagg
     6601 tcaagccgtg gtgcctaaag aagacacgca aaaattggcg atgctggaaa agcgtgcaca
     6661 ggaattaaag gaagagcttg ctgatttaga tcgtcgctat actcgcgaat atatggcgct
     6721 aacaccttcg ttaaaggtca ttccagagaa aatagcgacg ttacaaactg aaatcgatca
     6781 acttcgtagc gcgggccaaa ggattgtgct ttcagaagct gaacaagaat atgttgctgc
     6841 gcttcagacc acacaagcga ttcgggatca actagaagcg cataagaaaa aagcggctga
     6901 ttttacggca cgctttacgg agcatgaagc attaaaaatt gatctcgaag ggtttgagca
     6961 acgattacgc gaaacacaag aacgattgat ccaaatagaa accaagtatg gcgggaaatt
     7021 cccacaagtc gatgtagtgg aacgtgctgc ggtaccttta cagccaattt ggcccgatta
     7081 ttcacgagat tcactgattg ctctcataag ctcgctcatc atgggtttga ttgcggtttg
     7141 ggtggttgag tttttaaccc gcaaagagaa ggccaagtta gcgattaatc tctcaggggt
     7201 gcatatctat ggcggtgaag aatctgcgcg agaagtagtc aatcagttac cgctgacaca
     7261 gcaacgcatg ctagcgccgc aatcagttca gccagccttg atcggtggcg tcattcagga
     7321 aattgcccct cagaaaatcc acttgctctt tcaggcgggt aacaataaag aaaaacaact
     7381 catggtattg ctactgagtg ggttaaccct ggatgaaatt accctactag atccgcagtg
     7441 ttttgatctt gaaaataacc agctttccat cctaggcaaa tatccgagaa aaataccgct
     7501 tcatcccgca ttgcggcgat tagtcgaagc acgtggctat aacctaattg gagcatcagg
     7561 ccatgcactt aatctggatg atctggcggt tttgctgact tgcacaattg ttgatgccgg
     7621 cctgaccgca gcggagggca tttcagcaga tgcattacgg caaacctatc tgatttattt
     7681 ggttaggcaa ggaatgcggc tttcagatct ggcgcaaatc gtgggataca tcacctcgac
     7741 tgaattaacg acattgagtc aatttgcacc gttagggcct aagttagcag cgtcagccgt
     7801 taatttattt tatccgctgg aagcttccta gcagatcgtc gaatcgattt tatacctgac
     7861 acgatcaggt ttcattgcaa gcagttaatt tcttagccgg agggatatgc tacgaaaaat
     7921 ttttaccacg cttggttttg gggcttccgc gatgatgccg agcgagccgc cctacactcc
     7981 ctatcagagt gacgctgcca atgagattta taatttactg ttctgcgata atctagcagc
     8041 cttcaaaccc aaggaagggg agcgcgctgc tacttggcaa atcgtgctgt ttgctgaacc
     8101 ggctgatcga tctgcgttgc tggcattagc gaatgatgtt acgcaagaga gtcgcattcg
     8161 gtatctggcc tatacccgat tgcggttgct tggggaaaat gtgccaacca aagttttact
     8221 gggcgtgatc gttgaagttt ctcttgccga tggcctagac gcgcttgcgg cttattccga
     8281 cggcggagtt cgctatatca accatagcgg ggaattggta ttttttgaag gttccactga
     8341 gctcgagccc catgtgaaaa agcttttagc agcatctgag cctgtagttg cgcgtatcgg
     8401 cccatggaag aaaccacgcc gatctcctcc tgaatccgat aacgttcgac tcacgtttct
     8461 tgtgtccgat ggactttact tcggtgaagg acctatgtcc atcatgcaac atgaggaaat
     8521 ggctggaccg gtaatccagt ctgccaccac gcttttgcag actatcgttt ccagggcgag
     8581 tgaatgaagg catctaattt tgttgcatct ggtacgatag aatcaataat gtgttaatgt
     8641 aagacctgac ccctttgaca cacaacgata gtagatggcc cctgggattt cacaatgaca
     8701 gagctaataa cattttgaaa ctgtctagat agaggacaga atataggaaa aaatatggtc
     8761 gtgaataaaa ccaaggatcg tgcgataggg gcaatcatgg gcgcgttcat tggtgacgcg
     8821 ttaggtctgg gcccccattg gtactatgat ctcaacgaac ttcgtcgcga ttatggtgac
     8881 tggattgata actatactga ccccaggccc aatagatatc atgccggatg taaagcaggg
     8941 cagttatctc aagtgggatt tattctggtg cttactcttc gttcgcttgt tgaatgcggt
     9001 gaatataata aagatgactt ttgccggcga atggacgaag aactttttcc gttgcttgat
     9061 ggcacgccga tgagtggtcc cgggagttat accagccaat caattcgtga agcatggcga
     9121 tgccgggtgc agcataaact accgtggggg caaaccggga gtcatgccga tactaccgag
     9181 gcgattgaac gtacgctggc gattgcagtt cgttacgcgt ttcagccgaa tcaacttgca
     9241 gcagccatta ccgataacac gatgctgact cagattgatc ataccgttgt ttcgatgacg
     9301 gtcgcctatg gtgcagtact aggtctttta atccaaggac atctactcaa tactgatctg
     9361 tcaggaaagc tcatggagct agtcaaggat ggcaagttac cttttcatgc agtcacacga
     9421 gacgctttac agccgccccg ccctggtgac cctgatccgc cccgcgcagg ccgtttcgct
     9481 tctccagatg ctttacttac accgtcttat atggctgcag cttatgagaa tccggaaatt
     9541 cgcatagaac cggcatggaa agtttctatt gtctatggaa tgccatgcgc tatttatcat
     9601 cagctaccag cggcttatta tttggctgct agatttaagg atgattttga atcagctgtc
     9661 cttcatgcga ttaatggggg tggtcagaat caggcacggg cgattctcac gggtgcgctc
     9721 accggcgctc aaactggatt aaccggaatt ccgcaacgat ttcttgatgg gcttgatgga
     9781 tgtgctgatt tactcaggtt agctgagcat ctcgctgcta aggttggaaa tttttgagct
     9841 tatattgaag cttaattcct tcttatcatt ttggtttgag ttcaagccat caattagggc
     9901 aaaaaatgat gcgaggagat tcgtaaaata acttaacaat aagcttagaa tcgcgctgaa
     9961 ataagaaatt tatgtaattt tgcaaatgta tcatgtaata aattcatgca agatttgaat
    10021 tagacttcac cgtacgccat gagtatcggt ttgtgggggg ccgagggcat ggagcttgcg
    10081 caccgacacc tcgtgccatt gctttggctc agttcggcca tcccagatta gcgccctaac
    10141 atctgcggat gtaggtatgt cgccccaggt accgaaagta agaccatctg agcttggttc
    10201 ggccaaatag tgtgaccagt accgatagcg caaatagttg cggtactcat cattttccgg
    10261 gagcacacga taaagtactg acagcgaaca accgtccgtg cacgggaggc gcgaaagttc
    10321 tcgaaacgat gagttggatg acttgaatga aacgcctgac tgtagggatg atttgaggag
    10381 gcatatactc accacgtgat tatccgggca ttcgagtagt tcgacagact tacctggctt
    10441 tgacgtgaca ttgtctaaat acgaacctgg aaagaaaagt tcgacgagta tgacgcgttc
    10501 gtttcttgag aaagttgtgg gaacattcca gacactaaac aagcgactct tcccttccca
    10561 taaaggatct ccaatcagag attctcgaag acttgacgaa agaccgactt ggcactgtcc
    10621 agaggaatga aagctagcct tgtactggtg acctacgctt cgcggggcga gatacacttc
    10681 agatccttca atccatacgc gccaatcctg agagcagtcg gtggctgcgc gatgaactcg
    10741 gaagcggtaa gtgggtgttg tgctaatgtg agacctaact acaattaaac atccgatatg
    10801 acgcaatata ctactctaca aatgaaaaat aatatggaat tgttcatgtt attcccttat
    10861 gtttttttcg agctaaatat tcctcaattc tacgtagcta tttattatcc aacaacgcat
    10921 taataagctg ggattggagt gtctggaatt gttggttctg atcgcgctgt ggggaaggtt
    10981 tttgccaaag tgggttttgt tggagaatcg agacgatctc tgaccatttt gccggcaatt
    11041 ccccagtgac ggtttcttca atgtgatcgg gatcataagc agcgtgtccg cgctgcatca
    11101 attttgacag taattgttgc tggcgaagat acaaaatttt gagagtgttt tcgtcagcgc
    11161 ataaccattc tgttccttgg attttggcct tgatttcttt gccgtaacgc ttgaaggtag
    11221 accatcctgt tcccgctaag gcgcccaagg cactcgctgc ccctaagctc aaaccgccga
    11281 ccatcaaatc aatgcctgcc ccagcggctg cacctttgag tgcagcgctt ccagcatcga
    11341 gtccataagc tttgaggaca cccgg
//
LOCUS       DAOQLJ010000071        11225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2251_length_11225_cov_6.190331, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000071 DAOQLJ010000000
VERSION     DAOQLJ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 11225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11225
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2251_length_11225_cov_6.190331"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PK522_06385"
     CDS             complement(<1..993)
                     /locus_tag="PK522_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HNC41397.1"
                     /translation="MNVNQKNFSDLVHLEQLRHIETNKGSELSYASVADMRLHSAREF
                     SYRYLLNHLTTRAKRLIHDNALTEAVEQPHKLFVRATRICVIIAAILGALAVGHAVGE
                     SATLNIYWLITVLLGFNFLSILLWILGISFNLQSLRNGVVAQLACWLPYRNKDSDSIR
                     ALATRAWWETCLTGSIGKWRISTLTHQFWLAYLGAGLTFLILLMMAKQYDFIWGTTLL
                     PDNSLPKLTQFLGTPVEMIGFRSPDTQQIASSRVGLAIHDAETRSAWASFLLGMLLVY
                     GLFPRIILLSISLFMLRLAESRYKLDLYLPYYITLRQQLMSHEFEAQVIDADPQI"
     gene            1147..1854
                     /locus_tag="PK522_06390"
     CDS             1147..1854
                     /locus_tag="PK522_06390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HNC41398.1"
                     /translation="MKPVVIIRFFPIEGPGYFATFLDNNHIPWKVVKVDQAETIPSTL
                     QAFSGLVLMGGPMSVNDDLPWIPQVLGLIQQAIELNIPVLGHCLGGQLMTKAMGGVVT
                     QGPIKELGWGKVDVMDHPVAKAWLGDITEFEAFHWHGETFSIPEKATLLFSSPYCAHQ
                     AFATDIHLAMQCHVEVTEEMVPTWSTFNTAEIEQNLSSPAVQTAPAMQERLPQRIAAL
                     NGVADQLYRKWIQALKA"
     gene            1948..2790
                     /locus_tag="PK522_06395"
     CDS             1948..2790
                     /locus_tag="PK522_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNC41399.1"
                     /translation="MLWKGRRQSSNIEDRRGQYARRGGAIGGGSIIIALIAVFIFGQD
                     PLVVLQELQQQAGMVETSNSNYEPNPEEQQVFELISVVLADTEDTWNQIFQKAGYDYQ
                     APKLVLYSGMTPTACGTGQAASGPFYCPGDQKIYLDLSFLSELQRMGASGDLAVAYVL
                     AHEVGHHIQTLTGVSTKVRNLQNQTNQVGANAIQVKMELQADCLAGIWVNHTQKRTQF
                     LEQGDIEEALQAASAVGDDQLMRKAGLPPRREAFTHGSSKERMSWFKQGLKTGDLAAC
                     ETGI"
     gene            2841..3827
                     /locus_tag="PK522_06400"
     CDS             2841..3827
                     /locus_tag="PK522_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNC41400.1"
                     /translation="MRFHLIIAYILLNFSTLVIAQSPELGNSSFDKFEFALIGDVPYS
                     RDDFDKLDRLIHEINTDPRLTWVLHAGDIKNGISVCSDRMFIDRLQRFQQFKIPFILT
                     PGDNEWTDCHRFFTGSYNPLERLARLRGLFYANPGNSLGQKTVTLRTQASDPHFSEYV
                     ENLRWRKNHVVFATVHVVGSNNGWAQFSNRTEADDMEAKRRTEAAIVWIKQTFEEARN
                     TDARGVFVLFHADLNFEYAKGSHARLGFDGIIEVFEQESAQYLRPILLAHGDSHRFRV
                     DKPLQNNKKQTINHVTRVETFGASNVHWVRVAVDPASAEVFTIQKQIIKKNW"
     gene            complement(3863..4573)
                     /gene="pyrF"
                     /locus_tag="PK522_06405"
     CDS             complement(3863..4573)
                     /gene="pyrF"
                     /locus_tag="PK522_06405"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNC41401.1"
                     /translation="MSEPRIIVALDYSDKKQALDLVNQLDKTCCRVKVGKELFTAAGP
                     QLVEQLMMLGFEVFLDLKFHDIPNTVAQACKVAANLGVWMVNVHASGGKKMLLAARDA
                     LPPGKTKLIAVTVLTSLDQFALNEIGWYRAPMDIATQLASLTRDCGLDGIVCSAAEAA
                     IIRKNIPQKNFWLVTPGIRPADSAQHDQARVTTAKVAILNGADYLVIGRPITQSANPM
                     ATLQQLNDEILSACSTIN"
     gene            complement(4592..4801)
                     /locus_tag="PK522_06410"
     CDS             complement(4592..4801)
                     /locus_tag="PK522_06410"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNC41402.1"
                     /translation="MYIFIWLAGFVFFLLLLTFSAKNTDLVTVNYYFDFHWQVPLVVL
                     FLIFFALGSCFGYLSCFVKHLRKKT"
     gene            complement(4858..5151)
                     /locus_tag="PK522_06415"
     CDS             complement(4858..5151)
                     /locus_tag="PK522_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HNC41403.1"
                     /translation="MTKSELISRLSARYPQLLTKDTELAVKMILDAMANSLSKGERIE
                     IRGFGSFDLNFRPARVGRNPKSGEKVLVPEKYVPHFKAGKKMREHVDFDKEKS"
     gene            complement(5168..6883)
                     /gene="rpsA"
                     /locus_tag="PK522_06420"
     CDS             complement(5168..6883)
                     /gene="rpsA"
                     /locus_tag="PK522_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNC41404.1"
                     /translation="MTTAANLKDSSENFAQLFEESLARKEMRVGEVITAEIVRVDYNF
                     VVVNAGLKSESYIPIDEFKNDRGEIEVKAGDFVSVAIDALEDGYGETRLSRDKARKLN
                     AWHDLEEAMEKGTIVSGMVNGKVKGGLTAMVNGIRAFLPGSLVDIRPVKDTTPYENKE
                     MEFKVIKLDKKRNNVVISRRAVLEETQGADRESLLANLQEGSTVHGIIKNITDYGAFV
                     DLGGIDGLLHITDLAWRRVKHPSEVINVGDEVTAKVLKFDQEKNRVSLGLKQLTEDPW
                     VGLSKRYPQGTRLFGKVTNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVYPSKIVQLG
                     DEVEVMIIEIDEERRRISLGMKQCKPNPWEEFASGHEKGEKVSGQIKSITDFGIFIGL
                     PGNIDGLVHLSDLSWSQSGEEAIANYKKGDEVEAVVLSIDVEKERISLGIKQLEGDPF
                     TIYVAEHDKNSIVKGKVKAIDPKGAVIGLSEEVDGYLRASEITRDKVEDIRTLLKEGD
                     EVESMIINVDRKNRNINLSIKAKEQQEESSALQMVSSKTPASTGTTNLGALLKAKMDN
                     KNTEE"
     gene            complement(6991..7656)
                     /gene="cmk"
                     /locus_tag="PK522_06425"
     CDS             complement(6991..7656)
                     /gene="cmk"
                     /locus_tag="PK522_06425"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNC41405.1"
                     /translation="MDKIPVITIDGPSASGKGTVAQRVAKQLTFHYLDSGALYRLVAL
                     QAMRSKTDITDEHALAHIAENLVVCFNDSTIILNQEDVTDLIRSEECGKYASRIAAFP
                     LVRTMLLNKQRAFRQQPGLVTDGRDMGSVVFPNASLKIFLTASAEERAQRRYKQLIEK
                     GINANINILQQDIYERDARDSKREIAPLQQFADTKLLDTTFLSIDQAVNEVVKWYQAI
                     RNT"
     gene            complement(7686..8999)
                     /gene="aroA"
                     /locus_tag="PK522_06430"
     CDS             complement(7686..8999)
                     /gene="aroA"
                     /locus_tag="PK522_06430"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNC41406.1"
                     /translation="MGWLDLTISQCALGTVKLPGSKSISNRVLLLAALAQGDTLVQNL
                     LVSDDTRHMLDALKMLGISITQIDTVDYQIQGSGGKFPNQEAKLFLGNAGTAFRPLLA
                     VLAIMGGHYYLSGVSRMHERPIGDLVDALKQLHAEIQYLGNPGYPPLEIKLAEIHNTP
                     VTIKGNVSSQFLSGLLMALPLIKEATSIIVDGELISKSYVSLTLAQMEKFGVHVINEN
                     WQRFILPGLQTYQSPGKIYVEGDASSASYFLAAGAIGGGPVRVVGVDKDSAQGEIKFA
                     EALERMGAKITYGENWVESAAISCNPTTRYLSAIDLDCNHIPDTAMTLAVLALFAEGT
                     TKLRNIASWRVKETDRIAAMATELRKLGASVEDGQDYLVITPPKSITPNVLIDTYDDH
                     RIAMCFSLVSFAVPVRINNPECVAKTFPDYFQRFTAIAHPPIGSN"
     gene            9191..9436
                     /locus_tag="PK522_06435"
     CDS             9191..9436
                     /locus_tag="PK522_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41407.1"
                     /translation="MDESTDVLLISILAIPFVIAIQAWVKKRKDSRRNEEGEEEFKTT
                     REALFAFLLEGLAIVGGLAILIAATSGILRYVINTYS"
     gene            complement(9513..10490)
                     /locus_tag="PK522_06440"
     CDS             complement(9513..10490)
                     /locus_tag="PK522_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC41408.1"
                     /translation="MLNYIIRRICYAIPILIGVNLITFVLFFMVNTPDDMARMHLGVK
                     YVTDDAIEKWKQDRGYDKPLIYNQTAEGFSKLTQTIFFEKSVAMFVFDFGQADDGRDI
                     SHEIKSRMVPSLAIALPVFLLGIWVYISLALMMVFFRMTYIDFWGVVLCVALMSISSL
                     FYIIGGQFLISKIWHLVPISGYGTGLDASKFLLLPVVIGVISSIGANTRWYRTIFLEE
                     MNKEYVRTARAKGLPESTVLFKHVLKNALIPILTGAVVTIPLLFLGSLIAESFFGIPG
                     LGSYTIEAINSQDFAIVRAMVFIGSFLYIIGLILTDISYTLVDPRIRLM"
     gene            complement(10502..>11225)
                     /locus_tag="PK522_06445"
     CDS             complement(10502..>11225)
                     /locus_tag="PK522_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC41409.1"
                     /translation="RSIEEAKKLLAEAGYPDGIDIKTGAPLVLYFDATSRSADDKSIL
                     DWMRKQFKKINIQLVVRSTDYNRFQDKMRKGNAQIFEWGWNADYPDPENFFFLLYGPQ
                     SKAGNHSGNNAANYDNPEYNQLFERMEKMDNGPERQAIIDQMVEILRYDAPWLWGYHP
                     KDYALYHAWFQNVKPNKMSYNNMKYLRINAELRERKQSEWNQPVLWPIGLAVIILMLS
                     LLPGFIAYRQKERKTEAKQLVG"
BASE COUNT         3191 a         2329 c         2515 g         3190 t
ORIGIN      
        1 aatttgagga tcagcatcga taacctgggc ttcaaattca tgcgacatga gctgttgccg
       61 caaagtgatg tagtagggga ggtataaatc gagcttatag cgactttctg caagcctcag
      121 catgaaaagt gaaatgctca acaggataat ccgcgggaac aaaccataga cgaggagcat
      181 gcctaataga aaacttgccc acgcactgcg tgtttcagca tcgtggatag ctaggccgac
      241 acgactcgag gcgatttgct gtgtatcggg ggatcgaaac ccgatcattt caacgggcgt
      301 ccccagaaat tgggtcaatt tgggcaaact gttatcagga agcaacgttg tgccccagat
      361 gaaatcgtat tgtttggcca tcatcagcaa aatgaggaag gtaagtccag cccctaaata
      421 agcaagccag aactgatgcg tcaacgtgct gatccgccat tttccaatgg atccggttag
      481 gcaggtttcc caccaagcgc gagttgctaa tgcgcggatc gaatcactgt ctttattgcg
      541 ataaggtaac caacaagcga gttgagccac gacgccattc cgaaggcttt gtaaattaaa
      601 gctgatacct aaaatccaga gcagaatgga gaggaagtta aagcctaaca acacggtgat
      661 aagccagtaa atgttcagcg tcgcagattc ccccactgcg tggccaaccg ccaatgcgcc
      721 aagtatggct gcaataatga cgcaaattcg agtggcgcgg acaaataatt tatggggctg
      781 ctcgacagct tcagtcaacg cattatcgtg aatcaagcgt ttagcacgcg tcgtaagatg
      841 gttcagaaga tatcgatagc taaattcccg agcactgtgc aagcgcatat ctgcaacact
      901 tgcataggat aattctgatc ccttattggt ttcgatatga cgtagctgtt ctaagtgaac
      961 gagatcagaa aagttttttt gattaacatt cattgtgaaa gcgatttgct aaaggcgcta
     1021 cactttgcaa ttttgtaatg ggtgattaac aatggtaaag cactcaacat aaagaatttc
     1081 aaaagattaa tggaaagcgc gtgttataaa gattgaaacg tttgtaaaag ctagagaggc
     1141 ttgattatga aacctgttgt aattattaga ttctttccta tcgaagggcc cggttatttt
     1201 gccacatttc ttgataataa tcatatcccg tggaaagtgg taaaggtaga tcaggctgaa
     1261 actatcccat caacgctgca agcattcagc ggtctcgtgt tgatgggagg accgatgagt
     1321 gttaatgatg atttgccttg gattccacaa gtactaggtt taatccaaca agcgattgag
     1381 ctgaatattc cagtcctggg ccattgcttg ggagggcagc taatgactaa agcgatggga
     1441 ggcgtggtaa cacaaggacc gatcaaggag ctgggttggg gtaaggtcga tgtgatggat
     1501 cacccggttg caaaagcttg gttgggggac ataactgaat ttgaggcgtt tcattggcat
     1561 ggagaaacct tttccatacc tgaaaaagca acgttgctat tctcaagccc ttattgcgcg
     1621 catcaagctt ttgccactga tattcatttg gcgatgcaat gccatgtaga agtaacggaa
     1681 gagatggtgc ctacctggtc aacttttaat accgcggaaa ttgagcaaaa tttatctagc
     1741 cccgcagttc aaacagcacc agcgatgcaa gaaagacttc cacaacgtat cgctgcacta
     1801 aatggggtgg ctgatcaatt gtatagaaaa tggatacaag cgttgaaagc ttgaatgact
     1861 tcaaagtgtg atgcgtggaa gcgcttacac gggtcgatct gatatcatca atcctaaaga
     1921 aacataaaag ggcaaggaga gttagatatg ctgtggaaag gaaggcgaca aagtagcaat
     1981 atcgaagaca gacggggcca atatgcgagg cgtggtggcg cgattggtgg tggtagcatt
     2041 atcatcgcgc tcattgcggt gtttattttc gggcaagatc cattagtcgt gctacaagaa
     2101 cttcagcagc aagcgggcat ggttgaaaca tcgaattcca attatgaacc taaccctgag
     2161 gaacagcagg ttttcgaatt gattagtgtg gtacttgcag atactgaaga tacgtggaat
     2221 caaatattcc agaaagcagg ctatgactat caggcgccga aactggttct ttattcgggg
     2281 atgacgccga ccgcttgtgg gacaggtcaa gcagcgtcgg ggcctttcta ttgtccaggt
     2341 gaccagaaga tctatctgga tttatcattc ttaagtgagt tacagcgtat gggtgcaagc
     2401 ggtgatctgg ctgtggccta tgtgctggct catgaggtgg ggcatcatat tcagacgctc
     2461 acgggcgttt caactaaagt gcggaacctt cagaatcaaa ccaaccaggt aggcgcaaat
     2521 gcgatccagg ttaagatgga attacaagcg gattgtttag cgggcatttg ggttaatcat
     2581 acgcaaaaaa gaactcagtt tcttgagcaa ggcgatatcg aagaagcact acaagcggca
     2641 tctgcggttg gcgatgacca gcttatgcgt aaagccggac tgccacctcg gcgtgaagcc
     2701 tttacgcatg gttcttctaa ggaaagaatg agttggttta agcaaggttt aaaaaccggt
     2761 gatctagcgg cttgtgaaac cggtatttag cttctggata aatggtaatc aactgaatcg
     2821 atatcagatc aatgtatgtg atgcgctttc acttaataat tgcctatatt ttgctgaatt
     2881 tttctacgct ggtgattgcg caatccccag aattaggcaa tagctcattc gacaaatttg
     2941 aatttgcact cattggtgat gtgccctata gcagggatga ttttgataaa cttgatcgct
     3001 tgattcatga aatcaatact gaccctcgat tgacatgggt gctccatgcg ggtgatatca
     3061 agaacggcat atcagtttgc tcagatagga tgtttattga tcgtttgcag cgttttcagc
     3121 aattcaaaat tccatttatc ctaacgcctg gagataatga atggaccgat tgtcatcgct
     3181 tttttacagg gtcatataac ccgctggagc gcttagcgcg gctaagggga ttgttttatg
     3241 ccaatccagg aaatagccta ggacagaaaa cagttacgct acgaacacaa gcaagtgatc
     3301 cacacttctc cgaatatgta gaaaatcttc gttggcgcaa aaatcacgtc gtttttgcga
     3361 cagtacatgt cgtcgggagc aataatggtt gggcgcaatt ttctaatcgt actgaagctg
     3421 atgatatgga agctaaacgt cgcacggagg ctgcgatagt ttggataaaa caaacctttg
     3481 aggaagcgag aaatacggat gcacgcggcg tatttgtgct atttcatgca gatctaaact
     3541 ttgaatatgc gaaaggaagc catgcaagac tgggttttga tggaataatc gaggtatttg
     3601 agcaggagtc tgcgcaatac ttaaggccga ttttacttgc ccatggcgat tctcatcgtt
     3661 tccgcgtcga taaaccgtta caaaataaca agaagcaaac aatcaaccat gttacccgtg
     3721 tcgaaacctt tggtgcgagt aatgttcatt gggttcgtgt tgcagttgat ccagcatcgg
     3781 ctgaagtatt taccatccag aaacaaatca tcaaaaagaa ttggtagctt ggactgagtc
     3841 acgctgttga tcgagtcagt tgttaattga ttgttgagca agctgacagg atttcgtcgt
     3901 ttaactgttg aagggttgcc ataggattag ccgattgcgt aatcggccgt ccaatcacta
     3961 aatagtcagc gccatttaaa atagcaactt tggccgttgt aacgcgagct tgatcgtgct
     4021 gagcgctatc cgctggacga attcctgggg tcacgagcca aaagttcttt tgagggatat
     4081 ttttccgaat gatagcagct tcagcagccg aacaaacgat accatccaag ccacaatcac
     4141 gggttaatga agcgagctga gtggcaatat ccattggtgc tctataccaa ccgatttcat
     4201 tcaaggcgaa ttggtctaag ctggtgagca cggttactgc aattaatttg gttttgccgg
     4261 gtggtagcgc atcgcgtgct gcgagcaaca tttttttccc tcccgatgcg tgaacgttga
     4321 ccatccaaac gcctaggttt gctgcaactt tgcatgcctg tgcaacggta ttgggaatat
     4381 cgtggaattt caaatctaag aacacctcaa agccgagcat catcagctgc tcgactaatt
     4441 gaggtcctgc tgcagtaaat aattcttttc ctactttgac gcggcagcag gttttatcta
     4501 attgattgac taaatctaaa gcttgtttct tatccgaata atcaagtgca acgataatac
     4561 gaggttcact catataaggg cgagtagatc gctaagtttt tttacgcaaa tgcttaacga
     4621 agcaagataa atacccaaaa catgagccta gtgcaaaaaa gatcagaaat aacacgacga
     4681 gtggaacttg ccaatgaaaa tcgaaataat agttaactgt tacgagatca gtattcttag
     4741 cagagaatgt taataacaat aagaaaaaca caaaacctgc taaccaaatg aagatataca
     4801 taatcgtctt tcctttaaga ttgagtagat aggaacgcgc tctatgtagg tttcaaatta
     4861 agacttttct ttgtcgaaat caacatgttc tcgcatcttt ttacccgctt tgaagtgtgg
     4921 aacatatttc tcaggaacta gaactttttc accagacttg ggattccggc ctacacgcgc
     4981 aggccggaaa tttagatcaa agctgccaaa tccacgaatt tcaatacgtt cgcctttcga
     5041 taagctattg gccatggcgt ccagtatcat ttttactgct aactcggtat ccttggtcaa
     5101 taattgagga taccgggctg agagcctcga aatcaactca gattttgtca ttgatgcctc
     5161 ttacctatta ttcttctgtg tttttattat ccatttttgc ctttagcaag gcaccgagat
     5221 tggttgtacc tgtacttgca ggtgtttttg aactaaccat ttggagggca ctactttctt
     5281 cttgctgctc tttggctttg attgagagat tgatattacg atttttgcga tcgacattaa
     5341 taatcattga ttcgacttca tcaccctctt taagtagggt acgaatatcc tcaactttat
     5401 cacgcgtgat ttcagatgcg cgtaaatagc cgtcaacttc ttcagataag ccgataacgg
     5461 ctcctttcgg atcaattgct ttaactttac ccttcacaat actattttta tcatgctctg
     5521 ccacataaat agtaaaagga tctccttcaa gttgcttgat gccaagagaa attcgttctt
     5581 tttccacatc gatcgatagg acgacggctt ctacttcgtc tcccttttta taattagcaa
     5641 ttgcttcttc accggattgg ctccaagaaa gatcagacaa atggaccaag ccatcgatat
     5701 tgccaggtaa gccaataaaa ataccgaaat cggtaatcga tttgatttga cctgaaactt
     5761 tttcaccctt ttcatggcca gaagcaaatt cttcccaagg atttggtttg cattgtttca
     5821 tgcctagcga aattctgcgg cgttcttcat cgatctcgat aatcatcact tcaacttcat
     5881 cgcccagttg aacaattttt gaaggataga cattcttatt cgtccaatcc atttcggaaa
     5941 catgcactaa cccttcgatt ccttgttcta tctcaacaaa agcaccataa tcggttagat
     6001 tagttacttt gccaaataag cgagtgcctt gtgggtagcg tttggagagt ccaacccatg
     6061 ggtcttctgt taattgtttc aatccaagcg acacacgatt tttttcttga tcaaatttta
     6121 agactttcgc agtcacttca tccccgacat tgataacttc tgaaggatgt ttaacgcgac
     6181 gccatgctaa gtcagtgata tgcaatagac catcaattcc acctaaatcg acaaaggcgc
     6241 cataatcggt aatatttttg ataatgccat gaacagtgga accttcttgg aggtttgcaa
     6301 gcaatgattc gcgatctgca ccttgtgttt cctcaagcac tgcacgccga gaaataacaa
     6361 cattattgcg ttttttgtcc agtttaataa ccttgaactc catttctttg ttttcatacg
     6421 gagtcgtatc tttaactggg cgaatatcaa ccaatgaacc cggaaggaaa gcgcgaatcc
     6481 cattaaccat agcagtgaga ccgcctttaa cttttccgtt taccatccca ctgacaatcg
     6541 ttcctttctc catggcttct tcaagatcat gccacgcatt taactttctt gctttatccc
     6601 gcgatagtct tgtttctcca taaccatctt caagtgcatc aatagccacg ctaacaaagt
     6661 caccggcttt aacctcgatt tctccccggt cattcttaaa ttcatcgata gggatataac
     6721 tttcagactt taagcccgcg ttaacaacga cgaagttgta atcaacacga acgatttctg
     6781 ctgtaattac ttctcccaca cgcatttctt tacgtgcgag actttcttca aagagttgag
     6841 caaaattttc tgaggagtct ttcaggttag cggcagtagt cattataaaa aattacctaa
     6901 tttgttaatc aatccaatcc ccttataagc aggggattgt tggttattgg gttgaaaaaa
     6961 agattattta cttaaagaaa gaacgaagat ttatgtattg cgtattgctt gataccactt
     7021 aacaacttcg ttcacggctt gatcgatact cagaaaagtt gtatcgagta acttcgtatc
     7081 tgcaaattgt tgcaagggag caatttctcg tttgctatca cgcgcatctc gttcgtaaat
     7141 atcttgctgt aagatattga tgttagcatt tattcctttc tcgatcaact gcttatagcg
     7201 cctttgggca cgttcttcgg cgcttgcagt aagaaaaatc ttaagggatg cattggggaa
     7261 aacaacggat cccatgtccc gtccgtcggt gaccaatcct ggttgctgcc ggaatgcacg
     7321 ttgcttattt aataacatcg ttctgacgag tggaaaggcc gcaatacgag acgcgtattt
     7381 gccgcattct tcactgcgta tcaaatcggt gacatcttct tgattaagta tgatagtgga
     7441 atcattgaaa caaactacta gattttctgc gatgtgagcc aatgcgtgtt cgtcggtgat
     7501 atcggtcttt gaacgcatgg cttgtaatgc aacaaggcgg tagagcgctc cactatccaa
     7561 atagtggaat gtgagttgct ttgctacacg ttgtgctacg gttcctttcc ccgatgcgga
     7621 agggccatcg atggtgatga ctgggatttt atccatttta aaagagatct cttgaaaaaa
     7681 ataagttaat ttgacccgat cggcgggtga gcaatagcgg tgaatcgttg aaaataatca
     7741 ggaaaagttt tagcgacaca ttcagggtta ttaattcgaa ccggcacggc gaatgaaacg
     7801 agcgaaaagc acatagcaat gcggtgatca tcataggtat cgattaacac attgggcgta
     7861 atggatttgg ggggggtaat cactaagtaa tcttgcccat cttcaacaga tgcaccgagt
     7921 ttacgcaatt cagtggccat cgcagcaatg cggtctgttt ctttgactcg ccagcttgcg
     7981 atattacgta attttgtcgt tccctctgca aagagagcaa gcactgctaa agtcattgcg
     8041 gtatcaggaa tatggttgca atccagatcg atggcactta agtatcgtgt tgtggggttg
     8101 catgaaatgg ccgcagattc cacccagttt tcaccatagg taatttttgc acccattctt
     8161 tcgagtgctt cggcaaattt tatttcgcct tgcgcgctat ctttgtcgac accgacaact
     8221 cgtactgggc ctcctccaat agcacctgca gcgaggaagt aggaagcaga agacgcatcg
     8281 ccctcgacat aaattttccc tgggctttgg taggtttgta ggccaggtaa aataaagcgc
     8341 tgccagttct cattaatgac atggactcca aatttttcca tctgtgctaa ggtgaggctc
     8401 acataggatt ttgaaatgag ctcgccgtcc acgataatac tggtcgcttc tttgataagt
     8461 gggagcgcca ttaataatcc actcagaaat tgactggata cgttcccttt tatcgtaact
     8521 ggtgtattgt gtatttctgc taacttaatt tccagagggg ggtagcctgg attgccaagg
     8581 tattgaattt cagcgtggag ttgttttaac gcatcgacga gatccccgat tggacgctca
     8641 tgcatgcgtg agacacctga gagataatag tgaccaccca tgattgcgag taccgctaaa
     8701 aggggacgaa aagcggttcc cgcatttcct agaaataatt tagcttcctg attaggaaac
     8761 tttccaccgc ttccttgaat ctgataatcc acagtatcga tttgcgtgat gctaatgccc
     8821 agcattttaa gcgcatcgag catgtgtctc gtatcatcag ataccaggag gttttgcacg
     8881 agtgtatccc cttgagcaag tgctgcgagc aataagaccc gattggagat acttttagag
     8941 cccggcagtt taacggttcc aagcgcacac tgagaaatag ttagatcaag ccatcccata
     9001 gtttaaataa atttttttaa ttctttttct agtcattaac gctacttttt taaaggaaga
     9061 acaaagcatt caataagggg gaacgataga gatagagagc aggtattacg atgtctgcta
     9121 taatgaaaaa taatctagaa aaagttaatg ttttcaacca ataataatta tagcttaaag
     9181 gaacgatcga atggatgaat ctacagatgt gttactgatt tctatcttgg caatcccgtt
     9241 tgtcatagcg attcaagcgt gggtgaaaaa acggaaagat agtcgacgaa acgaagaggg
     9301 cgaagaagaa tttaaaacaa cgcgcgaggc tttatttgct ttcctgcttg aagggctagc
     9361 aattgtaggc ggcctagcca ttttgattgc agctactagc ggtattctta ggtatgtgat
     9421 taacacttat agttagactt gagatacttg ttgattagct gagttgatag ttggtagtag
     9481 gctattaatt tgacttcttg aaaggctgat cactacatta gccttattct gggatcgact
     9541 agcgtgtagg aaatatctgt cagaataagg ccgatgatat aaagaaacga accaatgaat
     9601 accatcgcac gaacgatggc aaaatcttga gagttgatag cctcgatggt atagctgccg
     9661 aggccgggaa taccaaaaaa tgactcagcg ataaggctgc ctagaaataa gagcggaatc
     9721 gtgacgactg cgcccgttag aattggaatg agggcatttt taagcacgtg tttaaatagg
     9781 acagtacttt caggtaagcc tttggcacgt gcggtacgaa catattcttt gttcatttcc
     9841 tccagaaaga ttgttcgata ccagcgagta ttagcgccta tactactgat taccccaatt
     9901 actacaggta ataacaaaaa tttactcgcg tcgagtccag taccatagcc tgaaataggg
     9961 actagatgcc agattttgct gattaagaat tggccgccaa tgatataaaa aaggcttgaa
    10021 atcgacatca gtgcgacgca tagtacgaca ccccagaagt cgatataagt catgcgaaag
    10081 aaaaccatca tcaaggcaag tgagatgtag acccatattc ccaacaaaaa taccggtaat
    10141 gcaatggcta agctaggaac catgcgagat ttaatttcat ggctgatgtc gcggccatca
    10201 tcggcttgac caaaatcaaa cacaaacatg gcaactgatt tttcgaagaa aatggtctgt
    10261 gttagctttg aaaagccttc agccgtttgg ttataaatca gtggcttatc ataacctcga
    10321 tcctgcttcc acttttcgat cgcgtcatcg gtgacatact tgaccccaag gtgcattctt
    10381 gccatgtcgt cgggtgtatt gaccataaaa aacaaaacga atgtaatgag attcacgcca
    10441 atcaaaattg gaatcgcgta acagatacgg cgaataatat aattaagcat tgtttacttt
    10501 tttaacctac aagttgcttg gcctcagttt tacgttcttt ttggcggtat gcaatgaacc
    10561 ctggtagtaa gctcagcatg agtataatta cagcgaggcc aatgggccaa agcaccggct
    10621 gattccactc gctttgcttg cgctctcgta attcagcatt aatgcgcaag tatttcatat
    10681 tgttataaga catcttattg ggttttacat tttgaaacca tgcgtgataa agcgcatagt
    10741 ctttggggtg atacccccat aaccaagggg catcgtagcg tagaatttcc accatctgat
    10801 cgataattgc ttgccgttcg gggccattat ccattttctc catgcgttca aataactggt
    10861 tatattctgg attatcgtaa ttggcagcat tattaccgct atggttgccg gccttacttt
    10921 gaggtccata cagcagaaag aaaaaatttt cgggatcagg ataatcagca ttccaacccc
    10981 attcgaagat ttgtgcgttt cctttgcgca ttttgtcttg gaatcgattg taatctgtgc
    11041 ttcttacaac caattggata ttgatttttt tgaattgctt gcgcatccaa tcaagtatgg
    11101 atttatcatc ggcgctccgt gaagtagcat caaaatagag aacaagtggg gcgccggttt
    11161 taatgtcaat tccatcaggg tatccagcct cagcaagtag ttttttggct tcttcaatgg
    11221 atctt
//
LOCUS       DAOQLJ010000072        10843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2354_length_10843_cov_6.579162, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000072 DAOQLJ010000000
VERSION     DAOQLJ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10843
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2354_length_10843_cov_6.579162"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PK522_06450"
     CDS             complement(<1..434)
                     /locus_tag="PK522_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011113005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HNC41410.1"
                     /translation="MPELPEVETTRRGITPALINQNITNVTIRHANLRWPISDRLDQL
                     LVGQRIEAITRRAKYLLLRCTRGFLIIHLGMSGSLRISFDSASVEKHDHFDLKVSSGA
                     VLRYRDPRRFGAILWSDAEKIAQHPLLVNLGVEPLSDAFDGK"
     gene            complement(434..1297)
                     /locus_tag="PK522_06455"
     CDS             complement(434..1297)
                     /locus_tag="PK522_06455"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNC41411.1"
                     /translation="MASRVWLNLIMFATAVGLAVFLYIKPSFQPQTTYPISSLSKEAV
                     QLIQISSQHGTIELKRSNGDWLMTEPLQARADKTKVERILDLLTTTSDYRLPPENLSR
                     YGLAAPIAQLQVNHELFAFGQIAPVSNHQYVMTQSGIFLISPRHAMLLTVAPTELIDR
                     RLLGEAEIPQEFIFKAMHLQQTEKWLLSSGASDVQPSQDEINQWVHSWQLAQASYITQ
                     NEHEVDETQETLTIVLQSGQPIHFLIMQRDPELVLLRKDQFINYHFLGHSGNQLLSPY
                     SGIRHEERMVE"
     gene            complement(1306..2658)
                     /locus_tag="PK522_06460"
     CDS             complement(1306..2658)
                     /locus_tag="PK522_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNC41412.1"
                     /translation="MTTITQKLRFNLLVQNGLFVLLLLIIIALAGYAAMQFRYQWDVS
                     QNNRNSLSQASLDVINKMNGPIGITVYATPQDVQLGDIRKIISNFLMIYQRVKPDLVF
                     EFIDPIEQPDLAQEAGIRMNGEIIITFDHRSEHLTAINEQAFTNALMRLVRSEEKRVM
                     VLSGHGERKLDGIANRDMGDFGKKLIETGFKGESLNLASSDIPLETNLLVIASPQTDL
                     LEGEVEKIITYLDQGGSLLWLVDQESLFGLQPLAEKLGITFTPGIVVDPQAKRLKAPV
                     TFALGTIYGQHAITENFDYITVFPFARQIILNENEEWYGVSLVEVAPQGWVETEALGD
                     EVSYDETHDVPGPVSVAVALDRTLDDREQRVVVIGSSHFLANTYLGNGGNIDFGVNII
                     NWLAGDDDLILIQPRATIDSQLVLSETSLTLIAVGFLIVLPLFLLVTGFVIWWQRRAS
                     "
     gene            complement(2679..3422)
                     /locus_tag="PK522_06465"
     CDS             complement(2679..3422)
                     /locus_tag="PK522_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNC41413.1"
                     /translation="MILTIAAKEIKVLFVSPLAWVFLAAIQLILTWVFLGRLDAFIEL
                     QSQLMLIANPPGATEVIIVPVYAVASIVLLMATPLLTMRLMAEEWRNHTMPLLMAAPV
                     TVTAIVLGKFAGLMIFFLIIIFLVGLLPFSLFLGGTMDLGLILSNTMGLLLLAASFVS
                     LGLYVSSLTTHPAIAAAGTLGLLMMLWIIDIVSTDLGNLLPNLSLLKHYEQFNRGMID
                     SFSVIFFMLFIISFLVLTIRRLDGSRLHG"
     gene            complement(3424..4170)
                     /locus_tag="PK522_06470"
     CDS             complement(3424..4170)
                     /locus_tag="PK522_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC41414.1"
                     /translation="MNETACTLIAHDLSRQFGQYAAVQGIEIELKQGEVLGLLGPNGA
                     GKSTTMQMLSGNLAPTRGSIQICGIDLLDHPEEAKQNIGYLPEIPPLYKELTVDEYLR
                     FVAKLHRVESSQILDALVRVKDRCGLNQVGKKLIGILSKGFQQRVAIAQAIIHQPKVI
                     ILDEPSVGLDPNQIREIRELIRELGKTASIILSTHILSEVETICDRVQILNKGGVVYS
                     AALSDLKQKSINLEQIFEQFTLKKELVGDY"
     gene            4603..5304
                     /locus_tag="PK522_06475"
     CDS             4603..5304
                     /locus_tag="PK522_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNC41415.1"
                     /translation="MKKIISAKLYLIGVSVLPLLMLSNMVAADAHNRQGESETEKTQG
                     YNVNDRGAVSRNSTGLCWRTGFWTEEMAIPECDPDDTPPVEKQAATPAPAPLTSPEKI
                     TLSADALFDFDKAVLKPQGKASLDEFVGKLNMISYDLVIAVGHTDRLGSDEYNKRLSI
                     RRAEAVKGYLVSKGISADRVFTDGKGESDPVTGDTCRGEKRTKALIDCLQPDRRVEIE
                     VAGTRSDRYETIEQK"
     gene            5367..6701
                     /locus_tag="PK522_06480"
     CDS             5367..6701
                     /locus_tag="PK522_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HNC41416.1"
                     /translation="MNVTSTALRIDTLLEARWIIPVDPSHTTLIDHAIAIQDGKIIAI
                     LPTTEALSQFSPKQHFVLNHHLIIPGLINTHTHAAMTLMRGMADDLPLMDWLKNCIWP
                     VEKQWVDTDFVLAGTTLACAEMLKGGITCFNDMYFFPEKAVEAAAQTGMRAAIGLISI
                     EFPTAYATDANDYLAKGLMLREQYLEYPTLSFCLAPHAPYTVSDSTLSNIFTFANQLD
                     LPIHMHLHETSEEIASSLHDFGIRPIERLQKLNLLGPNLIGVHMVHLTNEEINLLKLH
                     NCNVVHCPSSNMKLASGIAPIVDLVNASINVAIGTDGAASNNRLDMFEEMRLTSLLGK
                     VTHGAQALPAHQVLRMATLNGAITLGIEALTGSLSVGKAADITAIDFSGLELSPCFNP
                     LSHLIYVAGREHVSHVWVNGQLLLNEGELVTLNEAEIIERTQYWQNRITSKQ"
     gene            6712..7419
                     /gene="ubiG"
                     /locus_tag="PK522_06485"
     CDS             6712..7419
                     /gene="ubiG"
                     /locus_tag="PK522_06485"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HNC41417.1"
                     /translation="MDNNGINADPLELEKFSQLAHHWWDPNSEFKPLHEINPLRLNYI
                     DQVSSGLSGKSVVDVGCGGGILSESMAARGANVTGIDLSDKALKVAKLHLLESGHQVD
                     YRKITVEMLATEHPRHFDVVTCMEMIEHVPDPASVIKSCAQLVKPGGWVFFSTLNRNP
                     KAYLFAIIGAEYILKLLPRGTHEYAKFIKPSELAHMARDAGLTEARIIGMTYNPITKI
                     YALESDTSVNYIMAFQA"
     gene            7458..8105
                     /locus_tag="PK522_06490"
     CDS             7458..8105
                     /locus_tag="PK522_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNC41418.1"
                     /translation="MVDAILFDFDGTIADTAPDLGHALNRQRINRGLPSLPISIIRKQ
                     ASAGSRGLLYLGFNLVPTHPEYELMREEFLQFYTERLCHDTCLFPGIADILDHLDSSN
                     IPWGIVTNKPSRFTKPIVQSLGLINRAACIISGDDTPHTKPHPEPLLHACNQINAKPA
                     NCIYLGDDIRDVKASLAAGIKPIVARYGYLGNDGPPESWGADHLIDHPKELAHYL"
     gene            8342..8770
                     /locus_tag="PK522_06495"
     CDS             8342..8770
                     /locus_tag="PK522_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC41419.1"
                     /translation="MHHVKKWFCYFAIFYLFIDSCVADSTAPEHVQGAQTIDTLTAKL
                     FFDQKIPFIDVRKYEDFIAGHIPGAYHLSIKSDFDELRLRAIVDKESPVVIYCNGIYC
                     MGSSIAAQKAVNWGWKTIYYYREGMPAWKNNEYPIQAQQN"
     gene            8830..9411
                     /locus_tag="PK522_06500"
     CDS             8830..9411
                     /locus_tag="PK522_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNC41420.1"
                     /translation="MSKKKILMLVGDYVEDYEAMVPFQALQIIGHIVHTVCPDKKSGD
                     TVRTAVHDFEGDQTYSEKPGHLFRLNASFSKIKVEQYDALIIPGGRSPEYIRLNPKVI
                     KIVQHFAQQGKPIAAICHGAQLLVAADSVRGRTCSAYPTLEPDVKRAGGKYIDIPMDK
                     AHQDQNLVTAPGWPAHPDWLAKFLDLLGTRIKP"
     gene            9411..>10843
                     /locus_tag="PK522_06505"
     CDS             9411..>10843
                     /locus_tag="PK522_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC41421.1"
                     /translation="MEKEIKKQCDRCFRFDNSKIIWLGIVIVVGILSACSRPIWNSPY
                     PSEDASKNILYNAFAERPKHLDPVQSYSSNEILLTAQIYQPPLQYHYLKRPFTLIPQT
                     THGMPLVKYIDEIGNESHAEKDITNIAYSIYEISIKPDIRYQPHPAFARNDQGNLRYH
                     ALTQKETESIFKLSDFTALGSRELLAEDYAYQIKRLAHPKLHSPIFGLMSEYIVGLKE
                     YAETLRKIADTLPKGTHLDLRDFPLEGVEIVDASTYRIKIKGKYPQFIYWLAMPFFAP
                     IPWEAERFYSQPGLAEKNITLDWYPVGTGAYMLVENNPNRIMVLERNPYFQGEYYPAD
                     GMPEDEESGLLADAGKPLPFIDRVIFSREKENIPRWNKFLQGYYDASGIGSDSFDQAV
                     QLTSHGEATVTEEMQQQGIRLETAVAASTYYMGFNMLDPVVGGRTDQEKAAARKLRQA
                     ISIAVDYEEYVSIFANGRGIPAQGPIPP"
BASE COUNT         3278 a         2438 c         2146 g         2981 t
ORIGIN      
        1 atcttgccat caaaagcatc actcaacggc tctactccaa gattaactaa tagtggatgc
       61 tgcgctattt tctctgcatc actccataag attgccccaa agcggcgagg atctcggtaa
      121 cgcaacacag caccactact cactttaaga tcaaaatgat cgtgtttctc aacactagct
      181 gaatcaaacg aaatgcgtaa gctacccgac attcccagat gaataatcaa gaatccacgc
      241 gtacatctga gtaaaaggta cttggcgcga cgcgttatcg cttcaattct ttgtccaact
      301 aaaagttgat caagtctatc tgaaataggc cagcgtaaat ttgcatgtct tatcgttaca
      361 tttgttatat tttgattgat aagcgctgga gttatgcctc gtcgagtagt ttctacctct
      421 ggtaactcag gcattactca accattcttt cttcatgcct aatcccagaa taaggggata
      481 gtagctggtt gcctgagtga cccaagaagt gataattaat aaactggtcc ttgcgcaaca
      541 ataccaattc aggatcgcgc tgcatgatca aaaaatggat cggttgccct gattgcaata
      601 cgatcgttaa cgtttcttga gtctcgtcga cttcatgttc attttgagtt atataagaag
      661 cctgagccaa ctgccaggaa tgaacccact ggttaatttc atcctgactc ggctggacat
      721 cacttgcccc agaagataag agccattttt ccgtttgctg caaatgcatt gctttaaaaa
      781 taaattcttg tggaatttcc gcttcaccta ataaccgacg atcaattagc tctgttggcg
      841 ctaccgttag taacattgcg tggcgtggcg aaatcaaaaa aatacccgac tgcgtcatca
      901 catattgatg attagacact ggcgctattt gaccaaaagc aaataattcg tgatttactt
      961 gtaattgagc gataggtgcc gccaaaccat agcgactcaa attttccggt ggtagtcgat
     1021 aatcacttgt agttgttaat aaatctaaga tacgctccac ttttgtttta tcagctctag
     1081 cttgaagggg ctcagtcatg agccaatcac cattcgaccg ctttaattca atggttccat
     1141 gttgtgaact tatttgaata agctgaacag cttctttcga tagcgacgaa attgggtaag
     1201 ttgtttgcgg ttggaacgat ggcttaatat agagaaaaac tgcaagtcct acagcagttg
     1261 caaacataat caaattaagc caaacacgtg atgccatggc aaatactaac tagctctccg
     1321 ctgccaccaa atcacaaaac cagtaacgag caaaaataaa ggaagcacaa tcagaaatcc
     1381 gactgcgatc aatgtcaagc tcgtctcact caaaaccagt tgactatcaa tcgtggcgcg
     1441 cggttgaatc agaatcaaat catcatctcc agctaaccag ttgatgatat taacgccaaa
     1501 atcaatattg cccccatttc ctagataagt atttgcaaga aaatggctac tcccaattac
     1561 cacaactcgt tgctcccgat catccagcgt gcgatcaagc gccaccgcaa cgctaacagg
     1621 accaggcaca tcatgtgttt catcgtagct aacttcgtct cctaatgctt ctgtctcaac
     1681 ccagccttgc ggggcaactt caactaatga aacaccatac cactcctcgt tttcattcag
     1741 aataatctgt ctcgcaaaag gaaatactgt gatgtaatca aaattctccg taatcgcatg
     1801 ctgtccataa atcgttccca atgcaaatgt aacaggcgct tttaatcgct ttgcttgcgg
     1861 atcaactacg atacctggcg taaaagtaat ccctaatttt tcagcaaggg gttgtaaacc
     1921 aaataatgat tcttgatcga ctaaccacaa taaacttccc ccctgatcca ggtaagtgat
     1981 aattttttct acctctcctt ccagcaaatc agtctgaggt gatgcaatca ctaataaatt
     2041 agtctcaagc ggaatatctg aactcgccag attgagtgat tctcctttga atcccgtttc
     2101 tattaatttt tttccgaaat cgcccatatc gcgattagcg ataccgtcta gtttacgttc
     2161 gccatgtcct gatagaacca tgacccgttt ttcctccgag cgcaccaaac gcattaaggc
     2221 atttgtgaaa gcctgctcat tgatagcggt taaatgttca ctgcgatgat cgaatgtaat
     2281 gataatctca ccattcatcc tgattcctgc ttcttgagct agatcaggct gttcaatggg
     2341 atcgataaat tcaaacacca gatccggctt tactcgctga taaatcatta aaaaattgct
     2401 aatgattttt ctaatatcac ccaattgcac atcttgaggc gttgcataca ccgtgatccc
     2461 aataggacca ttcattttat ttatgacatc aagactcgct tgactaagac tatttcgatt
     2521 gttttgacta acatcccatt gatagcgaaa ttgcattgct gcatatccag ctagcgctat
     2581 tattatcaaa agcaatagga caaatagccc attctgcacg agcagattaa agcgtagttt
     2641 ctgggtaatc gttgtcattc tttggttata actgttgatt atccatgcag tcgactccca
     2701 tccaagcggc ggatagtcag taccaagaaa ctaataatga atagcataaa gaaaataaca
     2761 ctaaatgaat caatcatgcc tcgattgaat tgctcataat gcttaagcaa tgatagattc
     2821 ggcagaagat ttcctaaatc ggttgagaca atatcaatta tccatagcat catcagtaaa
     2881 ccaagcgttc ctgctgcagc aatggctgga tgagttgtta agctggaaac gtacagcccc
     2941 agggaaacaa agcttgctgc taagagtaat aaacccatcg tattactcaa tatcaaacct
     3001 aaatccatcg tcccacctaa aaaaagtgag aagggaagca atccaacaag aaaaataatg
     3061 atcaggaaaa agatcattaa cccagcaaat ttacccagaa caatcgctgt cactgtaaca
     3121 ggcgccgcca tcaacaaagg cattgtgtga tttcgccatt cttcagccat caaacgcatc
     3181 gttagcaaag gtgttgccat caataacacg atggatgcta ctgcgtaaac aggaacgatt
     3241 atcacttcgg tcgctccggg cgggttggca atcagcatga gctgcgattg tagctctata
     3301 aaagcatcca agcgccctag aaatacccat gtaagtatga gctgaatcgc agcaagaaaa
     3361 acccacgcga gaggggaaac aaataatact tttatctcct tggcagctat cgttaaaatc
     3421 actttaataa tctccaacca gctctttttt tagcgtgaat tgctcaaaaa tctgctcgag
     3481 attgatgctc ttttgtttaa gatctgaaag cgcagcacta taaacaacgc cccctttatt
     3541 taaaatttgt acacgatcac agattgtttc aacttctgac aagatgtgcg tagaaagaat
     3601 aatacttgct gttttaccca gctctcggat tagctcacga atttctctta tttggttagg
     3661 atccagaccc accgaaggtt catccaaaat aatcactttc ggttgatgaa tgatcgcctg
     3721 ggcaatagca actcgctgct gaaaaccttt tgataaaatc ccaataagtt ttttccctac
     3781 ttggttgagt ccacagcgat ctttaacacg caccaatgca tcaagtatct gtgacgattc
     3841 aacacggtga agtttagcta caaaccgtag atattcatcg acagtaagct ctttatagag
     3901 tggtggtatt tctggtaagt acccaatatt ttgtttggct tcttctggat gatcaagtag
     3961 atcaatgcca caaatctgga tcgaacccct ggttggcgca agatttcctg aaagcatctg
     4021 catcgttgta ctttttccag caccattcgg accaagcagc ccgagcactt caccttgctt
     4081 caattctatt tcaatacctt gcacagcagc atattgaccg aattgtcggc ttaagtcatg
     4141 agcaatgagt gtacaagcgg tttcgttcac aggattagat actcttcagt aatcaagcca
     4201 tcaacattca gtgttggata aagcgcaaca acacctgaac tttaaatcgt aagaaagtca
     4261 tcgtcgttaa gttaaaacta ttcgcagcag atcatcagga ttagctagaa aagctcttgc
     4321 tgttttcgca ttaacctaat ttttagatac cggataattg tcggagatat tagttgtgta
     4381 ttcaatcact ctgtcttaat cgcaagacag agtgttgtat atcaacaaca acctggtttg
     4441 acaaaaagca aaacatgtac gcataatccg gcccaagtag cgtgacgaat cggttcagag
     4501 atatgtatca tgggttcatg ttcatgtcgg tgttcagtgg tttcgttaaa ccaattggtt
     4561 ttgaagcaaa attacagaat tttttcgaaa aaggacgata gcatgaaaaa gattatatca
     4621 gccaaattat atctaatagg agtatctgtt ttacccctac tgatgctttc aaatatggta
     4681 gcagccgatg cacacaatag acaaggtgaa agcgaaaccg aaaaaacaca aggctataac
     4741 gtcaatgatc gtggtgccgt atctcggaat tccacaggtt tatgctggcg cactggtttc
     4801 tggacagaag aaatggcaat tccagaatgc gatcctgatg atacaccacc agtagaaaag
     4861 caagcagcta cgcctgctcc ggcaccttta acatctcctg aaaaaattac actttcagca
     4921 gacgcattat tcgattttga taaggcggtt cttaaaccac aaggcaaagc atcgctagat
     4981 gaatttgtag gcaagttgaa catgattagc tacgatctcg tgatcgcagt tggacatacc
     5041 gatcggctcg gatccgatga atacaacaaa cgtttatcaa taagacgtgc tgaagctgtt
     5101 aaaggctatc tcgtatctaa aggtatcagt gcagatcgtg tctttactga tggaaaaggt
     5161 gaatctgatc cagtaactgg cgacacctgc cgtggtgaaa aacgcaccaa agcattaatt
     5221 gattgcctac aacccgatcg tcgcgttgaa atcgaagttg caggcacacg aagcgatcgc
     5281 tacgaaacca ttgaacagaa ataaactcaa gtaaaatagt gagtagctta agccctgcct
     5341 tgtgcagggc ttttttatga gtaagtataa acgtgacatc tacagccttg aggattgata
     5401 cattactcga agcacgctgg attatcccag ttgatccctc ccatacaacc ttaattgatc
     5461 acgccattgc gatccaagat ggcaagatca tcgctattct tccaaccact gaagcgctat
     5521 cgcaattctc acctaaacaa cattttgttt taaatcatca cctcataatt ccaggtctga
     5581 ttaacacaca cacacatgct gcgatgacct taatgcgcgg catggctgat gatctcccgt
     5641 taatggattg gttaaaaaac tgcatatggc ccgttgaaaa acaatgggtt gatacggatt
     5701 ttgttctagc aggaacaact ttagcgtgtg cagaaatgct gaaaggaggc attacttgct
     5761 tcaatgacat gtatttcttc cctgaaaagg cggtggaagc ggccgctcaa accggtatgc
     5821 gtgcggcgat tggacttatc agtattgaat ttcccactgc atatgcgacg gatgcaaatg
     5881 attatctcgc caaaggactc atgctccgtg agcaatactt agaatatcct actctctctt
     5941 tctgtttagc gccgcatgcg ccttataccg ttagcgatag cacactgtct aatatcttca
     6001 cgtttgccaa tcaactggat ctaccaatcc acatgcacct gcatgaaacc agcgaggaaa
     6061 ttgcaagcag tctccatgat tttggaattc gtcctatcga gcgtttacaa aagcttaatt
     6121 tacttgggcc taatctgatt ggcgtgcata tggttcattt aaccaatgaa gaaatcaact
     6181 tattgaaact gcacaattgc aatgtagttc actgcccttc ttctaatatg aagcttgcga
     6241 gcggtattgc gcccatcgtt gacttagtca acgcatcgat caatgtcgca attggaacag
     6301 atggagctgc tagtaataat cgactcgata tgtttgaaga aatgcgcctc acatcgttac
     6361 tcggaaaagt cactcacggc gctcaggcac tgcccgctca tcaagtatta cgaatggcga
     6421 cgttaaatgg tgcgattact ctgggtattg aagcgctaac tggctcatta tcggttggta
     6481 aagctgcaga cattactgca atcgattttt ctggcttaga gctatcccct tgtttcaatc
     6541 cgctttccca cctaatttac gttgctggcc gagaacacgt cagccatgta tgggtaaacg
     6601 gtcaactctt gctgaacgaa ggagaacttg ttactctgaa cgaagcagaa attatcgagc
     6661 gcacgcaata ttggcaaaac cgcatcacaa gtaaacaata aaaggagaaa aatggataat
     6721 aatggtatca atgcagatcc attagagctt gaaaaattta gccaactcgc tcaccactgg
     6781 tgggatccca atagtgagtt caaaccattg catgaaatta acccactcag gttgaattat
     6841 atcgatcaag tcagcagtgg cctgagtgga aaatctgtag tggatgtcgg atgcggcggt
     6901 gggatcttat cagaatctat ggcagctcga ggtgcaaatg tcaccggaat tgatttaagc
     6961 gataaagctt taaaagtagc taaattacac ttattagaaa gcggccatca ggtcgattat
     7021 cgtaaaatta ccgtagagat gcttgctacc gagcatcctc ggcattttga cgtcgtaacc
     7081 tgcatggaaa tgattgaaca tgtacccgat ccagccagcg tcattaagtc ctgcgctcag
     7141 cttgtgaaac ctggtggttg ggtattcttt tctaccctca atcgcaaccc caaagcctat
     7201 ctttttgcaa ttattggggc tgaatatatt ctgaagttat tgccacgcgg aacacacgag
     7261 tacgctaaat ttatcaaacc ttctgagctt gctcatatgg ctcgtgacgc tggattgact
     7321 gaggccagaa taattggcat gacctataat cccatcacaa aaatttatgc gctggaatct
     7381 gacacgagtg ttaactatat catggctttc caagcctaat taatgcttta aatcttacca
     7441 aacactgcca acaacacatg gtcgacgcta tcctatttga ttttgatgga acaattgctg
     7501 atacagcacc tgatttaggt catgctctca atcgacaacg cataaaccgt ggcttaccgt
     7561 cgctcccgat tagcatcatc cgtaaacaag cttctgctgg atcccgcggt ttactctacc
     7621 ttggtttcaa tctagtgccg actcatcccg aatatgaact catgcgcgaa gaatttctac
     7681 agttctacac cgaacgtctt tgtcacgata cttgcttatt tccgggtatt gcagatattt
     7741 tagatcatct tgattcaagc aatattccat ggggtattgt aaccaacaaa ccctctcgat
     7801 ttaccaaacc catcgtgcaa tcgcttggcc tcataaaccg cgctgcctgt attatcagtg
     7861 gagatgatac accgcatacc aaaccgcacc cagaaccact actgcatgcc tgcaaccaaa
     7921 tcaacgctaa acctgcaaat tgtatttatt taggtgatga tatacgtgac gtgaaagcga
     7981 gccttgcggc tggaatcaaa ccgattgtcg cccgttacgg ttacctcggt aacgatggtc
     8041 cgcctgagtc ctggggcgca gatcacttga tcgatcaccc caaagagctg gcccattatc
     8101 tataaaaaca tattatggta ttgcgtgaaa agatacttgg taaagatttt caacaatacg
     8161 tcagtcaatt taaacacaaa ctttgttagt cattgattta ataaactccc tatcagtaga
     8221 ataactttag cacgcaacct ggattaatct atcctcacgg tgaatttctt ggcagatttt
     8281 catcaacaat ttgataatct cagttttgtt aatacaaata acactttata ggaagcttaa
     8341 catgcatcat gtaaaaaagt ggttttgcta tttcgctatt ttttacttat tcattgattc
     8401 ctgcgttgct gactcgactg cccctgagca tgttcaaggt gctcagacaa tcgacacctt
     8461 aactgcaaaa ttattctttg atcaaaaaat tcctttcatt gatgtgcgta aatacgaaga
     8521 cttcattgcg gggcatattc ctggcgcgta tcatttgtca atcaaaagtg atttcgatga
     8581 actaagatta cgcgcgattg tagataaaga aagtccggta gtaatttatt gtaatgggat
     8641 ctattgcatg ggcagttcga tagctgcgca gaaagcagtc aattggggct ggaagacaat
     8701 ttattattat cgtgaaggaa tgccagcgtg gaaaaataac gagtacccca ttcaggcaca
     8761 gcaaaattaa tttgaatccc taaatttttc atcgataacc tcattgcgta ataaatacag
     8821 gcgtttgtta tgagtaaaaa gaaaattttg atgctagtgg gagactatgt ggaagattat
     8881 gaagcgatgg ttcctttcca agcgctacaa ataattggac acatagtgca tacggtatgc
     8941 ccagacaaaa aatcgggaga taccgttcga acagcagttc atgatttcga aggtgatcaa
     9001 acttactcgg agaaaccagg ccacttgttt cgattgaatg cgagcttctc caaaatcaaa
     9061 gtggaacaat acgacgcatt gatcattccg ggaggacgct cacccgaata tatccgactg
     9121 aaccccaaag taatcaagat tgtgcagcat tttgctcagc agggtaagcc aatcgcggct
     9181 atttgccatg gcgcacaatt attagtagca gccgatagtg tgcgaggtag aacatgctct
     9241 gcctatccca ccttggagcc agacgtcaaa cgcgcgggtg gaaaatatat cgatattcct
     9301 atggacaaag cgcatcaaga tcaaaatttg gtaacggcac ccggatggcc tgcccatcca
     9361 gattggctcg caaagtttct agatctactg ggaacgagaa tcaaacccta atggaaaaag
     9421 aaatcaagaa gcagtgtgat cgttgtttta ggtttgataa cagcaaaatt atttggcttg
     9481 ggatcgtaat cgttgttggg atactatctg cctgtagtcg accaatttgg aatagcccct
     9541 accccagtga agacgcaagt aagaatattc tctacaacgc gtttgctgaa cgacctaagc
     9601 acctggatcc cgttcaatct tacagttcca atgaaatttt actcaccgca cagatttatc
     9661 aacccccact acaatatcac tacctaaaac gcccttttac gctgattcca caaacaaccc
     9721 atggaatgcc tctcgtcaaa tacattgatg aaataggcaa tgaatcacac gcggaaaaag
     9781 atattacaaa tatcgcctat tcgatttacg aaatttcgat aaaacctgat attcgctatc
     9841 aaccccatcc agcatttgct agaaacgatc agggaaacct tcgctatcat gcattaacgc
     9901 aaaaagaaac tgagtcaatc tttaaactga gcgactttac cgcattgggc tcccgcgaat
     9961 tgctagcaga agactatgct taccaaatca aacgattggc gcatcctaaa ttacattccc
    10021 ccatttttgg cttaatgtcg gaatatattg taggactcaa ggagtatgct gaaacactga
    10081 gaaaaatagc agatacatta cctaaaggca cacacttgga tctacgcgat tttccattag
    10141 aaggagtgga aatcgttgat gcttctacct accgaatcaa aatcaaagga aaataccctc
    10201 aattcatcta ttggctagct atgccttttt ttgcgcccat tccttgggaa gcagaacgat
    10261 tttattctca gccagggtta gctgaaaaaa atatcacgct cgattggtat ccagttggaa
    10321 ccggtgctta tatgcttgta gagaacaacc ctaatcgcat catggtactc gaacgtaatc
    10381 cctactttca gggtgaatat tatccagcag acggtatgcc tgaagatgag gaaagtggtt
    10441 tactcgctga tgcaggaaaa cctttaccct tcatcgacag agtcattttt agtcgtgaaa
    10501 aagaaaatat accgcgctgg aataaatttt tacaaggtta ttacgatgca tccgggattg
    10561 gttcggatag ttttgatcag gcagttcagc taacaagtca tggcgaagca acggtaacag
    10621 aggaaatgca acaacaaggt attcggctgg aaaccgctgt tgctgcatcg acctactata
    10681 tgggttttaa tatgcttgat cctgttgtag gaggccgaac agatcaagaa aaggctgctg
    10741 ctagaaaatt acggcaagcc atttcaattg cggttgacta tgaggagtat gtctccattt
    10801 ttgccaatgg gagaggaata cccgcccagg ggcctatccc tcc
//
LOCUS       DAOQLJ010000073        10749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2370_length_10749_cov_5.638208, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000073 DAOQLJ010000000
VERSION     DAOQLJ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10749
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2370_length_10749_cov_5.638208"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PK522_06510"
     CDS             <1..199
                     /locus_tag="PK522_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNC41422.1"
                     /translation="ISADSHAPISWMTQNGVPLNVIQEMGAWESAEMVRRYAHLAPEQ
                     FAQHARVLDGVLNGTNLAQSK"
     gene            complement(235..310)
                     /locus_tag="PK522_06515"
     tRNA            complement(235..310)
                     /locus_tag="PK522_06515"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(275..277),aa:Thr,seq:cgt)
     gene            complement(462..2648)
                     /locus_tag="PK522_06520"
     CDS             complement(462..2648)
                     /locus_tag="PK522_06520"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNC41423.1"
                     /translation="MTIVRVALNVPIDSLFDYRLPDASLQDIGRLVRVPFGKRRMVGV
                     IVERVQDTQIKPEQLKAVDEVLRSIPPLPALLLKLFIFCNHYYHHPIGMIAMGCLPAK
                     LAGVKSISKINTEQAFFMLTEAGRLVSIDTLPVRDKAQRSLLTQLQTVGTLTSKQAKA
                     ISRRAPQLLGLFLTRAWIEEQHSVTPSIQPKRVSMPTLNAEQADAVKSIVAELHQFKT
                     WLLHGITGSGKTEVYLQVIAAVLQQDRQVLILVPEINLTPQLEKGFRDRFPALSVICL
                     HSKLNTTERTQNWLLAQAGTANIVLGTRLSIFTPLPKLGLIIVDEEHDPSYKQQDGFR
                     YSARDVAIFRAKQTGVPIVLGSATPSLESYHNAVKGRYSLLTLRTRAIKNAALPVIQL
                     IDTRSKLLQEGLSEPMLNALEKNLAQNKQSMVFINRRGYAPILLCKSCAWMATCSRCS
                     SRLVFHLQKQLLCCHYCGHQQPSPHACPHCGDPDLLPFGQGTQRIEAAIAKHFSSARI
                     VRVDRDNISRKGAWAHIRQAIQAQQVDILIGTQLLAKGHDFPELSLVCILNADASLYS
                     TDFRAEERLFSQLMQVAGRAGRAAIVGTVLIQTEIPDHPLYAALIKHDYDHYAKKLLK
                     ERQTIDFPPYAYQALLNAEAPKLDSALNFLKNALTITPLAQSMQIYDPVPAQMPRLKG
                     YERAHVLIQSHSRKKLQDFLANWYTELLKIKKTKVRWTIDVDPLEF"
     gene            complement(2809..4146)
                     /locus_tag="PK522_06525"
     CDS             complement(2809..4146)
                     /locus_tag="PK522_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HNC41424.1"
                     /translation="MKLNQQTRKIGLIISGLCVLSLTACEDSKEVVEVKTKVTAKGPT
                     TGVLSDGFAISGVAYSTASGSSGVTDANGVFHYQHGDNVRFKLGELTLADIKGSAIVS
                     PIDLAAGNENKLRNLLILLQSLDSDDDVTNGISISSKVANAIKSSINLESDPATFSDN
                     AALKAAREAAELPGEAKSLEEAGEQFLARGINLLSSHIWVKYDNQSASALQIAEDGSG
                     EYLHGEATADDSCDENRVCGGETIFRTGVESGTTKAEAFDTRGFKLVGTTSIDTNLRA
                     GLSHPRATWRINTDGDELIISDIVVAQRERQQSSLFGELFHIAKPIELSADDEPIATE
                     VKERRYSKMDNTAQGIVGAWSLDKDTVKSKLFLFFPNNRFMLIDPTGQTVQSEPLACG
                     KPGIEFAAYSYDGASKALKITSFIFDTNACAGLSESADKLVTFDISSDGIRLL"
     gene            4429..5535
                     /locus_tag="PK522_06530"
     CDS             4429..5535
                     /locus_tag="PK522_06530"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNC41425.1"
                     /translation="MSNPAELPHLTEKFLSLPIDWYLNQQIYEIEKQRLFEQGPGYIG
                     HELMIPNLGDYYVLKWLNNSKVLVRNEHGIELLSNICRHRQAILLEGHGNTKNIVCPI
                     HRWTYAMDGKLMGAPFFNRNPCLDLGKASLQHWNGMLFAGARNVVQDLANLSVLKDFD
                     FSNHVLNHIQIDHYPCNWKTFIEVYLEDYHVEPYHPGLGHFVDINNLTWEFNDWYSVQ
                     VVGTNPRFDRAGSPVYEKWHQQVLRYNDNQHPPHGAIWLVYYPNIMLEWYPHTLIVST
                     IIPTGIESCTNIVEFYYPEEIALFESEFIEAEQAAYRETALEDREICRRMTEGRRALY
                     QQQINEFGPYQIPMEEGMAHFHQFLRREIGPFIR"
     gene            5587..6438
                     /locus_tag="PK522_06535"
     CDS             5587..6438
                     /locus_tag="PK522_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC41426.1"
                     /translation="MHSLWMLVAGFMFACMGLFVKLGAAHFTSTELVFYRSLFGLIVT
                     FLFLTSCRMPLTTVHWKIHCWRGLFGLGGLLLFFYCLTQLPLATAVSLNNTWPLFLII
                     LATLILKEHLHWPLACAVMLGFTGVLLLLQPTLNENQLFAGLLGVISGFLASIAHFNV
                     KQLGKLGESDWLVVFYFTLISTIITGIWLTLTQFSTITPHNLLLLIGVGLTATLAQLA
                     LTRAYRMGKTMVIGALAYSTVLFSGLWDVYFWEEVLPISAWAGMGLIILGGILSIRVV
                     PKGIRNS"
     gene            6493..6846
                     /locus_tag="PK522_06540"
     CDS             6493..6846
                     /locus_tag="PK522_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HNC41427.1"
                     /translation="MINIEKTQNVVVVAVIGEFTLTDYKEFEDKVLHQYHFDGRANLL
                     FDWRDMVDYSVDVAWEEIKFMREHGSEFGRVAVVTDDQWQTWSAWVSNLFIDADVTVF
                     SDYDEALDWVTDAEV"
     gene            6884..7366
                     /locus_tag="PK522_06545"
     CDS             6884..7366
                     /locus_tag="PK522_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41428.1"
                     /translation="MDEFALARVLHVLGVVLWIGGVAMVTTVLLPSVRQFKSEHEQIE
                     FFEQVESRFATQARFTTLLVGVSGFYMTYLLDAWSRFAEPRYWWMHAMVFVWVMFSIM
                     LYILEPLVLHKWFINRARVKPQATFNLILRMHWVMLSISLITTAGAVAGSHGWFPLGF
                     "
     gene            7366..7743
                     /locus_tag="PK522_06550"
     CDS             7366..7743
                     /locus_tag="PK522_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HNC41429.1"
                     /translation="MSYTLLKLIHVTSVSISFCLFLLRGLWMVNASDMLKRRWVKILP
                     HVNDTVLLLSAIMLAITIQQNPLENSWLTAKVIGLLTYILLGMVALRFGKTRRTKITA
                     WIMALMVFGYIVLVALTKNPTIF"
     gene            complement(7954..8160)
                     /locus_tag="PK522_06555"
     CDS             complement(7954..8160)
                     /locus_tag="PK522_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41430.1"
                     /translation="MQNFIGSFFRIHVDAFQHDLGADFDRVEVTGASHFGCTGTLFDN
                     IAFFIDQFIPQLLASRKRSVLDDV"
     gene            8533..9408
                     /locus_tag="PK522_06560"
     CDS             8533..9408
                     /locus_tag="PK522_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41431.1"
                     /translation="MFDDLAAYYHENVVSSFVEYRDVCNDGVAGRSRDLRAALNAASA
                     LFHLREHLPGGLLSRADVERLCPDYALLGDVVNASKHKTLNNKTPHGAPLVTDAGNLG
                     EQLVLIEYEDDAGTYRYIQKTVVVRLSDGSECSLLEILTSVINFWEGQMLSLEVLSKA
                     RVFTHDNDIRYRSRADCEANRLTFELVKGLRFHQTMRLLRFNKTTGRAEPIDLTGLDV
                     RSRIYQPKYDIELSLIHDASGKTYKTEITLTEEESLILSRMSSDEERRAYVNNLPSAQ
                     AALQQLAVGAGLPKG"
     gene            complement(9492..>10749)
                     /locus_tag="PK522_06565"
     CDS             complement(9492..>10749)
                     /locus_tag="PK522_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNC41432.1"
                     /translation="ISLMNSKFGIPSLEAPTIDMTQARYNLAGALDNPIKGFEKLKVR
                     TGYNDYKHDERERTGELGTRFKNRELETRVELLHTPINELKGAVGVHFQDRTFSALGE
                     ESIVPITHARSTGVFLLEERSWEKLRVEMGGRYEHATRNPQHNINPARSFDLFSGSIG
                     GFWNLIQDYNVGLTATHGQRAPAIEELYVNGAHHATATFQTGDATLRKEASNNIDLSI
                     NKATGPIRWKINGFYNHFNDYIFLRNSDRDRNGIADRVDEEGILDPAGEFLVQDMTQR
                     NATFYGAEAEANLTLKPETLELRLFTDYVRGKLNSGGNIPRVTPQRFGMELNYVKGPW
                     SGNLTTIYVLRQNKRAELETSTADYVLLNLEASYRIKQTKADGIRLFIQGRNLLNEEI
                     RYHTSFLKNFVLQPGVALVVGIRGDF"
BASE COUNT         2951 a         2412 c         2270 g         3116 t
ORIGIN      
        1 catctcagca gattcccatg cccccatttc ttggatgacc caaaacggtg tgccgcttaa
       61 tgtcatccaa gaaatggggg catgggaatc tgctgagatg gtgagacggt atgctcactt
      121 agcacctgag caatttgctc aacatgctcg ggtcttagat ggtgtgctca atggcacaaa
      181 tttggcacaa tcgaagtaaa aaggaatcag tagttacgct aagttgttga tttttggtgc
      241 tgttgagagg agtcgaacct ccgacctact gattacgaat cagttgctct accaactgag
      301 ctacaacagc tttgctgcat gcaagtgctg gaattataga tgcttcatca attaccagca
      361 atgattcttc tatttaattc gcttaaccca cttcagaatg agttgtgcaa aataaattga
      421 gttattccat tttaggcaat tccaaagatc aaactaaacc cctaaaattc aaggggatca
      481 acatcgatag tccaacgcac ctttgttttt tttattttca atagctcggt ataccagtta
      541 gccaagaaat cttggagttt ttttcgcgaa tgcgattgaa tcaacacatg cgcccgttca
      601 tagcctttga gccttggcat ttgtgcagga acaggatcat aaatttgcat agattgcgct
      661 aaaggcgtga tagtcaaagc attctttaaa aagtttagcg cgctatctag tttaggcgct
      721 tcggcattca atagcgcctg ataagcataa ggtgggaaat cgatagtttg tctttctttg
      781 agtaattttt ttgcataatg atcgtaatca tgcttgatta aagcagcgta aagcgggtga
      841 tcgggtattt cggtttgaat gagtaccgtc cctactatgg cagcccgccc tgcgcgaccg
      901 gcgacttgca ttaactgtga aaacaatcgt tcttccgctc ggaaatctgt gctgtaaagg
      961 gaagcatccg cgtttaagat acacaccaac gataattcgg gaaaatcatg gcctttggcg
     1021 agtaattggg ttccgataag gatatcaact tgctgcgcct ggattgcttg gcgtatgtgt
     1081 gcccaagcac ctttgcgact gatattatct cgatccacgc gtacaatcct ggcagatgag
     1141 aaatgcttgg caatcgctgc ttcaatacgc tgtgttcctt gaccgaatgg tagtaagtca
     1201 ggatcaccac aatgtgggca agcatgtggg gatggttgct gatgaccaca gtaatgacag
     1261 catagcaatt gtttctgaag atgaaacacc aaccgactcg aacaacgtga acaagttgcc
     1321 atccaggcgc aggatttaca cagaaggatg ggcgcataac ctcggcgatt gatgaacacc
     1381 atactttgct tattctgcgc cagatttttt tcgagcgcgt ttagcattgg ctcagataaa
     1441 ccttcttgaa gcaatttgct ccgggtatca atcaattgaa tcacgggtaa tgctgcattc
     1501 ttgatcgctc gcgttcgtag cgttaataac gagtaacgcc cctttaccgc attatgatag
     1561 ctttccagcg agggtgtggc tgaacctaaa acaatcggaa cacccgtctg tttggcacga
     1621 aaaatcgcca catcacgcgc tgaatagcga aaaccatctt gctgtttata ggatgggtca
     1681 tgctcttcat cgacaatgat caggcctagt ttcggtaatg gcgtaaatat cgaaagccgt
     1741 gtcccaagaa cgatgttagc cgtaccggct tgagctagca gccagttttg agtgcgctcg
     1801 gtggtattca gtttactgtg cagacaaatc accgataaag caggaaagcg atctcgaaac
     1861 cctttttcaa gctgaggggt tagattgatt tcaggcacta aaattaatac ttggcgatct
     1921 tgttgtaaga cagcggcgat aacctgcaga tagacttcgg ttttaccact gccggtaatc
     1981 ccatgcaata accaagtttt aaattgatga agctctgcca caatggattt gactgcatct
     2041 gcctgctcag cattgagcgt cggcattgat actcgcttag gctgaatcga cggggtcaca
     2101 gagtgttgtt cctcaatcca cgcccgtgtc aaaaacagcc ctagtagctg cggtgcacgc
     2161 cttgaaatgg ctttagcttg cttgcttgtc agcgtgccca ctgtttgcag ctgtgtaagt
     2221 aggctacgtt gcgctttatc acgtaccggc agtgtatcga tcgataccaa ccggcctgcc
     2281 tcagtgagca taaaaaatgc ttgctcagta ttgattttac ttatcgattt cacacctgct
     2341 agctttgcag gcaaacaccc catggcaatc attccgatcg gatggtggta atagtgatta
     2401 caaaaaataa acagcttaag caacaaggct ggcagaggcg gaattgatct taaaacttca
     2461 tcaaccgctt ttaattgttc aggcttaatc tgagtatctt gaaccctctc cacaataaca
     2521 ccaaccatac gccgctttcc aaatggaacg cgaaccaatc ggccgatgtc ctgtagcgaa
     2581 gcatcgggta accgataatc gaacaaacta tcaatcggga cattgagtgc aactcgaaca
     2641 atcgtcactt atcgttactc aatcacatca ctacaagatt agttttagaa aacaaaaagg
     2701 caagagattt actcttgcct tttaatgaat cagtaagatc gagaattaaa tgctcacaac
     2761 attaattcga tacacgagac aatttcaccg ctgcttgacc ctgctttgtc aaagtagccg
     2821 tattccatct gagctgatat caaaagtaac caatttatca gcactttctg ataaaccggc
     2881 acaggcgttg gtatcaaaaa taaaacttgt aatttttaat gccttactcg cgccatcata
     2941 actatacgca gcaaattcaa tacccggttt accacacgcg agcggttctg attgaaccgt
     3001 ctgtcctgtc gggtcgatca gcataaatcg attattggga aaaaataaga acaattttga
     3061 tttgaccgta tctttatcca aactccatgc gccaacaatg ccctgtgcgg tattatccat
     3121 cttggaataa cggcgttctt ttacctctgt tgcgatcggc tcatcatccg cgctaagttc
     3181 aatcggttta gcaatatgaa ataattcacc aaataaacta ctttgttgtc tttcgcgttg
     3241 tgcaacaacg atatccgaga taatcaattc atcaccatcg gtattaattc gccaagtcgc
     3301 acggggatgt gataatcctg ctctaaggtt ggtatcaatt gatgttgttc ccaccagttt
     3361 aaatccgcgt gtatcaaaag cttcagcttt ggtcgttcct gactccacac ctgttctaaa
     3421 aatcgtttca ccaccacaaa cacgattctc atcacaggaa tcgtctgcag tcgcttctcc
     3481 atgtaagtac tcaccactcc catcttcagc gatttgaagg gcgcttgcgc tttggttatc
     3541 gtatttaacc caaatatgac tgcttaacag attaataccc cgggccagaa attgttcgcc
     3601 cgcttcctcc aatgatttag cttcccctgg taactcagcg gcttcacgcg cagctttgag
     3661 agcagcattg tcagaaaagg tagctggatc agattccaaa ttaatggacg atttgattgc
     3721 attggcaact ttggatgaaa ttgaaatacc attggtgaca tcatcatctg aatccagtga
     3781 ttgcaataaa atcagcaagt tccttagttt attttcatta ccggcagcca gatcaatcgg
     3841 gcttacgatt gctgaccctt tgatatccgc gagggtcaat tcacccagtt tgaagcgaac
     3901 attatcgcca tgctgatagt gaaaaacacc gtttgcatcg gtaacgcctg atgaaccaga
     3961 agccgtacta taagccacac ctgaaattgc aaatccatcg ctaagaactc cagtcgtagg
     4021 cccctttgcg gtcacttttg ttttcacctc aacaacttct ttactatcct cacaagcagt
     4081 caggcttaaa acgcatagac cagatataat taaccctatt tttcttgttt gttgattcaa
     4141 tttcatcaaa gtgactcttc ccaatttagt taatcaatag atgcgatatg tatagcgtac
     4201 ctaaaattgt aatcttatga aataatagga taatatcgcg ctcagatttt ttgttaatct
     4261 ttaggattat tataaccctc aaaacaaaac ctagctgaat gataaattca catccagcaa
     4321 aaagtataga tgtaatttat ttttttgatt ttttgaatgc ttatacactt agttggtcaa
     4381 tcaaactttg aaagttgata acatcatttg ataattaact gattgtttat gtcgaatcct
     4441 gctgaattgc cccatttaac cgaaaaattt ctgtcacttc ctatcgattg gtatctcaat
     4501 cagcagattt acgagattga aaaacaacga ttgtttgaac agggtcccgg ttatattggc
     4561 cacgaattga tgattcctaa tctgggtgat tactatgtct tgaaatggct gaacaattca
     4621 aaagtattag tgcgtaatga acatggtatt gagttgctct caaatatttg ccgccatcgt
     4681 caggccattt tgcttgaagg ccatggtaat accaaaaata tcgtttgccc aatccatcga
     4741 tggacctatg cgatggatgg aaagttaatg ggtgcccctt tttttaatcg aaacccctgc
     4801 ttagatctgg gtaaagccag tttacagcac tggaatggaa tgttatttgc tggcgcaaga
     4861 aacgtggtcc aagatcttgc taatttgagc gtgctaaaag attttgattt ctccaatcat
     4921 gtgttaaatc acatacaaat cgatcattac ccctgcaact ggaaaacgtt tatcgaagtt
     4981 tacctggaag attatcatgt cgagccctat catccaggtc taggtcattt tgttgacatc
     5041 aataacctta cttgggaatt taacgattgg tatagcgtac aagtcgtagg gactaatccc
     5101 agattcgatc gggctggctc cccggtttat gagaagtggc atcagcaagt gttacgttat
     5161 aacgataatc agcatcctcc tcatggcgca atctggttag tttattatcc caatattatg
     5221 ttggaatggt acccacatac cttgattgtt agcacgatca tcccgactgg tattgagagc
     5281 tgtaccaata ttgtcgaatt ttactaccct gaagaaattg ctttatttga aagtgaattc
     5341 attgaagcag agcaagccgc ctatcgagag acggcactgg aagaccgtga aatttgtcga
     5401 cgcatgactg agggacgacg tgctctatat cagcaacaaa tcaatgaatt tggtccttat
     5461 caaatcccaa tggaagaagg catggcccat tttcatcagt tcctacgacg cgaaattggt
     5521 ccctttatcc gctaaaagac ttacagtttc cttcatcatt ttccttttgt aagcgattaa
     5581 tttcccatgc actcattatg gatgctcgta gcaggcttca tgtttgcttg catgggcttg
     5641 tttgtgaaat taggcgcggc acattttacc agcactgaat tagtatttta ccgttcactg
     5701 tttggcttaa tcgttacgtt cctatttctg accagttgcc gtatgccatt aacgactgtg
     5761 cactggaaaa ttcattgttg gcgtggattg tttggattag gcggattgct actctttttt
     5821 tattgcctca ctcagcttcc cttagcgacg gcagtttctc tgaacaatac ctggccctta
     5881 tttttaatta tcttagcgac ccttatactc aaagaacatt tacactggcc gctcgcttgc
     5941 gcggtaatgc tgggctttac tggcgtttta ttactattac aacctacgct taatgaaaat
     6001 caactattcg ctggtttgtt aggcgttatt tccggttttt tggccagtat cgcgcatttc
     6061 aatgtaaaac aattaggaaa actcggtgaa tccgattggc tcgttgtttt ttatttcacc
     6121 ttgatatcga ccattatcac cggcatttgg ttgactctga cccaatttag tacgattaca
     6181 ccgcataatc tgctgttatt gattggggtc ggcctaaccg caacgttagc gcaattggct
     6241 ttgacacgcg cttatcgaat gggtaaaaca atggtcattg gtgcactcgc ctatagtacg
     6301 gtattattct ctggactatg ggatgtttat ttttgggagg aagtactgcc tatttcagca
     6361 tgggcaggaa tgggattaat cattttaggt ggaatattaa gcatccgagt ggtgcccaag
     6421 ggtatccgca attcctaatg aacggatgcg tggtacccat tcataaaaat agaacttggg
     6481 agtatttaaa tcatgatcaa tattgaaaaa acccaaaacg ttgtggttgt cgctgttatt
     6541 ggggaattta cgctcacaga ttataaagag tttgaagaca aagttttaca tcaataccat
     6601 ttcgatggtc gtgctaattt gttattcgat tggcgtgaca tggttgacta ctctgtcgat
     6661 gtcgcttggg aagaaattaa atttatgcgt gaacatggca gcgaattcgg tcgtgttgcc
     6721 gttgtgacag atgatcaatg gcaaacctgg agcgcttggg tttccaatct tttcatcgat
     6781 gctgatgtaa ccgttttttc agattacgat gaagcgctgg actgggttac tgacgcagaa
     6841 gtttaaataa cccacctaat cagttgagct gaacaaaagg cttatggatg aatttgcgct
     6901 agcgagagtc ttacatgtgc ttggcgttgt actttggatc ggcggtgtag ccatggtaac
     6961 aactgtttta ctgccctcag tacgacaatt taaatcggaa cacgagcaaa tagaattttt
     7021 tgaacaagtt gaaagccgtt ttgcaacaca agcccgattt acaacgctcc ttgttggtgt
     7081 cagtggtttt tatatgacct acctattaga tgcttggagc cgtttcgctg aacctcgata
     7141 ctggtggatg catgcgatgg tttttgtatg ggtaatgttt tcaatcatgt tgtatatcct
     7201 agaaccgctc gtgctccaca aatggtttat caaccgagcc cgtgtgaaac cgcaagcaac
     7261 atttaattta attcttcgca tgcattgggt catgctgagt attagcttaa ttaccacagc
     7321 aggtgccgtt gcaggcagtc atggctggtt tcctctaggt ttctgatgag ctataccttg
     7381 ttaaaactaa tccatgtaac gagtgtatcg atcagcttct gcctattcct actccggggt
     7441 ctatggatgg tcaatgcatc ggacatgctg aagcgacgtt gggtcaaaat cttaccgcat
     7501 gttaacgata ccgtattgct gctaagcgcc atcatgctag caatcactat ccagcaaaat
     7561 ccacttgaaa attcatggct gacggcaaaa gtaataggat tattgaccta catcctactc
     7621 ggtatggttg ccctacgttt tggtaaaacg cgaagaacaa aaattaccgc ttggatcatg
     7681 gcattgatgg tatttggtta tatcgtatta gttgctttga ccaagaaccc tacaattttt
     7741 taacaatacc ctgaaattta ctgagccacg ccaaatttgc gatagattga attgacctgt
     7801 cgccatgaac gacataaact taaaacgtgt cgcaaaaatg cagaaattaa accatgacag
     7861 tttggcgccc ggatcaacct tacaacaatc ttccgctttt gcctcccata accgagctag
     7921 aaacacacac ggtacttaag caagcagccg aactcataca tcgtcaagaa cagaccgttt
     7981 tcgacttgcc aaaagctggg gaataaattg atcaataaaa aacgctatat tatcaaatag
     8041 tgttccagtg cagccaaaat gtgaagcgcc agttacttcg acacgatcaa agtcagcgcc
     8101 caggtcatgc tgaaaagcat ctacatgaat ccgaaaaaag ctgccaataa aattttgcat
     8161 aagggaaaaa attccctaag aaggaaaaaa aacttcactt tttaccaaaa taatgcatag
     8221 caactgttat ttagatattt ttcccgtcac cacaggaaaa tttgcacgat tgagcagcgt
     8281 tctttactcg gatatttgct ctcattgcgt agtagactca aaactgaggt ttttttctgt
     8341 ttgattattc attcaatttg tggcgctaaa caagtctgat aaaatgacag ctaaattggc
     8401 tattagctga gaaatataag gaaatatcat gaacaacccc gtattagata tcatttgtga
     8461 cgtaatatat tgcgtcattt cggacgtcta attgaagttg ggcgcttgac atccccacga
     8521 gactgaatcg ccatgtttga tgatctagca gcctattatc acgagaatgt cgtctcgtct
     8581 tttgttgagt accgcgatgt ttgtaacgat ggggttgctg gtcgcagtcg tgacctccgc
     8641 gcggcactaa acgctgcctc agcactcttt catctacgag agcatttgcc aggtgggtta
     8701 ctgtcgcgag ccgacgtcga gcgcctttgt cctgactatg cgctcctcgg cgatgtcgta
     8761 aacgcatcga agcacaaaac actgaacaac aagacccccc atggcgcacc gcttgtcacc
     8821 gatgccggga acctgggcga acagctcgta ttgatcgaat acgaagatga tgcaggaacc
     8881 taccgatata ttcagaagac agtagttgta aggctctctg acgggtcaga atgtagcctg
     8941 ctcgaaattc ttacgagtgt catcaatttc tgggaaggcc aaatgttgtc tctggaagtg
     9001 ctttcgaagg cgcgtgtgtt tacacacgac aacgacattc gctatcgatc gcgcgccgat
     9061 tgcgaagcca accgccttac ctttgaattg gtgaaagggc tacgatttca ccaaactatg
     9121 cgacttctgc gcttcaataa aacgacaggc agagcggagc ctatcgatct aactggcttg
     9181 gacgtgcgat ctcgcatcta ccaacccaag tacgacatcg agttgtctct aatccatgat
     9241 gctagcggga agacctacaa gactgagatc accctgacgg aggaagaaag tttgatcctt
     9301 tctcgcatgt caagcgacga ggaacgccga gcatacgtaa acaaccttcc ctccgcacaa
     9361 gccgctcttc aacagcttgc agtcggagcc ggtcttccga aaggataagt gagggcctcc
     9421 gtaaatgcga cgcataacaa agtacccgca tagtttaaca aatcgatcga acgcacgggt
     9481 tctccactcc tctaaaaatc acctcttata cccacaacaa gggcaacacc gggttgtagt
     9541 acaaaattct tcaaaaagga ggtatggtag cgtatttctt cattgagcaa attcctacct
     9601 tgaataaaaa gccgaatgcc atctgctttg gtctgcttaa tccgataact tgcttcaaga
     9661 ttgagcaata catagtcggc ggtactcgtt tctagctctg cacgcttgtt ctggcgtaac
     9721 acatagattg tcgtcaaatt acctgaccag gggcctttta cataattgag ttccatccca
     9781 aagcgttgcg gcgtaacacg gggaatattt ccgccactat tcaatttacc ccgaacatag
     9841 tcggtaaaaa gccgcaattc tagcgtttca ggtttaagcg ttaaattcgc ttctgcttcc
     9901 gccccgtaaa aggttgcatt tctttgcgtc atatcttgaa ccaaaaactc ccctgccgga
     9961 tcaaggattc cctcttcgtc gactcggtcg gcaatgccat tccgatcgcg atcggaattt
    10021 cttagaaaga tgtagtcgtt gaaatgattg taaaagccat taattttcca ccgtattggg
    10081 ccagttgctt tattgatgga taaatcaatg ttattggatg cttctttacg taacgtagcg
    10141 tcaccagttt gaaacgttgc tgttgcatga tgcgcgccat tcacataaag ctcctcgatt
    10201 gctggcgcac gctgcccatg tgttgcggtc aatcccacgt tgtaatcttg gattaaattc
    10261 cagaatccac caatggagcc actgaataaa tcaaatgatc gagcagggtt gatattatgc
    10321 tgcggattcc gagtcgcatg ttcatagcgc cctcccatct ctaccctcag tttttcccag
    10381 ctccgctctt ctagcaaaaa aacacctgtc gagcgcgcat gggtgatggg cacaatggat
    10441 tcttccccaa gcgctgaaaa agtccgatct tgaaagtgca cccctacagc gccttttagc
    10501 tcattaatcg gcgtgtgcag tagttcaacg cgtgtttcca gttcacgatt tttaaagcgg
    10561 gtacccaatt ctccagtacg ttcgcgttcg tcgtgcttgt agtcattgta acccgttcgt
    10621 actttgagtt tttcaaagcc cttaatggga ttatcaagcg ctccagcaag attgtaacgc
    10681 gcttgcgtca tatcgatggt tggcgcttct aagctcggga taccaaattt actattcatc
    10741 agtgaaatt
//
LOCUS       DAOQLJ010000074        10738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2371_length_10738_cov_6.149958, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000074 DAOQLJ010000000
VERSION     DAOQLJ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10738
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2371_length_10738_cov_6.149958"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PK522_06570"
     CDS             <1..338
                     /locus_tag="PK522_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41433.1"
                     /translation="GNFMNSDSIAVRKADASSANALQVNIRMQPGEHTGQPIFSNLTS
                     VQASQGVVVIDFGFIEPQMIQALSRLAQSGEKAPDTISAKMSCRMVVSVEAASQLAQQ
                     LSQLFVKKT"
     gene            364..783
                     /locus_tag="PK522_06575"
     CDS             364..783
                     /locus_tag="PK522_06575"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNC41434.1"
                     /translation="MNQSNPLPVKIKIQQDERSGKPSYANFTTVQHGQGKIVIDFGFF
                     DPKLLQVSVNVDNKPLGVIDAHLSRRIILSQEVATQFAQQLSQLFTKHGDVKEPQSES
                     VMTDTLPSEIQDHSPEQGENLSENPKKGFRFPWFKKK"
     gene            complement(822..1100)
                     /gene="infA"
                     /locus_tag="PK522_06580"
     CDS             complement(822..1100)
                     /gene="infA"
                     /locus_tag="PK522_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNC41435.1"
                     /translation="MAKEELIEMNGTVDKVLPDSRYLVTLENGHQLVAYTAGKMKKNH
                     IRILAGDKITIELSPYDISNGRITFRHIENRNPAPTKHHASFNKNTRK"
     gene            complement(1213..1416)
                     /locus_tag="PK522_06585"
     CDS             complement(1213..1416)
                     /locus_tag="PK522_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNC41436.1"
                     /translation="MAMGTVKWFNDEKGFGFITPENGSTDVFVHYSAIQGGGFRTLRE
                     NQRVSYDTSPGPRGPQAVNIRPA"
     gene            complement(1659..2555)
                     /locus_tag="PK522_06590"
     CDS             complement(1659..2555)
                     /locus_tag="PK522_06590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC41437.1"
                     /translation="MNNLRLTRLWPGLVGLFLLISPLTSTATIIRLQTTLGVIDIKLH
                     DETAPRTVANFFEYLKSNAYDRSFFHRTIPGFIIQGGGYVWNQTTNAANTIAKLSPVD
                     NEFSVTRSNVRGTIAMAKLPDKPNSATSEWFINLADNSADLNKQNEGFTVFGQVLGNG
                     MQVADAIAALPRTNAGSVFSTLPIIGTIEDHKLQEKNLVIVSRITSNKTDTPENDSDR
                     LFSYLEAFYSQYLSPANPLGSGQTVSATFENYYYRYYPTTQSYVGIANGEVYYLGPAS
                     GNQIISIGTFSNWLEQAVAAGY"
     gene            complement(2582..3043)
                     /gene="ssb"
                     /locus_tag="PK522_06595"
     CDS             complement(2582..3043)
                     /gene="ssb"
                     /locus_tag="PK522_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNC41438.1"
                     /translation="MASVNKVILVGNLGRDPEIRYMPSGDAMANLNIATTDTWKDKEG
                     EKQEKTEWHRVVMFGKQAEIAGEYLKKGSQVYIEGRLQTRKWTDKSNIERYTTEIVAD
                     RMQMLGSRSGGASYEPTDKDGEYNSANFSDKPTGQSKKSDRFDDLEDDIPF"
     gene            complement(3046..4431)
                     /locus_tag="PK522_06600"
     CDS             complement(3046..4431)
                     /locus_tag="PK522_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC41439.1"
                     /translation="MSSLPNSEKMSPLEKRASVGLASVYGLRMFGLFIILPVFALFAE
                     HLPGGDNFTLVGIALGAYGLTQAILQIPFGWLSDRIGRKPVIYLGLVLFAIGSFIAAT
                     AVDIYWVIAGRVIQGAGAISAAVMALAADLTREEHRTKAMAMIGMTIGATFAISLMIA
                     PLLNQWIGVPGIFIMTGLLAIIAIIVVHKIIPDPIVSRFHSDAEVSKSRFSTALRHPE
                     LLRLNYGIFALHAVLMALWLVVPLSLREAGLAADHHWQMYLPVLVLSVLLMVPAIIYG
                     EKKAKLKPVFITAIALLTIGQGLIAFTSQSLWGIGIALVIFFTAFNLLEASLPSLISK
                     IAPVEAKGTAIGIYSSTQFLGAFVGASLGGYLYQHVGLTAIIIFCTMLLLVWLYFAFT
                     MKSPAAVRSRMYHVAEMDNQRANGLSKELAALPGVHEALVLANEQVAYLKVDLKGFDE
                     EKVVQLIKGGA"
     gene            4498..7329
                     /gene="uvrA"
                     /locus_tag="PK522_06605"
     CDS             4498..7329
                     /gene="uvrA"
                     /locus_tag="PK522_06605"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNC41440.1"
                     /translation="MELIKIRGARTHNLKNINLDIPRNKLVVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQLMEKPDVDLIEGLSPAIAIEQKATSHNPRSTVGTVT
                     EIHDYLRLLYARVGDPFCPEHGIKLSAQSVSQMVDHVLRLPADTKLMVLAPSVVGRKG
                     EQQDLFDELRAQGFTRIRLDGSIYEIDQLPKLTKNKKHTIEVVIDRLKVSSEAKQRLA
                     ESFEMALRHGDGRAIAVEMETGQEHLFSAKFSCPVCNYALSELEPRLFSFNNPVGACP
                     KCDGLGQITFFDPARVVTLPHLSLAAGAIKHWDRRNQFYFQMLTAIAKHYEFDIETPF
                     EQLADTIKQIILFGSGKEKIQFTFLNESGRASKELYPFEGVIPNLSRRYKETDSQTVR
                     EELAKFINSRICPDCEGTRLCREARHIRVSEQAIYQISAWPLKQASAFFDQLVLAGHK
                     QEIAERIIKEISNRLQFLNNVGLDYLSLDRSADTLSGGEAQRIRLASQIGSGLTGVMY
                     VLDEPSIGLHQRDNGRLLDTLQQLRNLGNSVIVVEHDQDAIFLADHVVDMGPGAGEHG
                     GCIIAQGAPSEIQHHPASLTGQYLSGKRSIAIPAVRTPLNPERILTLSGVTGNNLKQV
                     ELNLPVGLLVCITGVSGSGKSTLINDTLYRAVAQQLYGNNTEPAPYQTLEGIAFFDKV
                     INIDQTPIGRTPRSNPATYTGLFTPIRELFAGVPQARERGYTPGRFSFNVKGGRCESC
                     QGDGVIKVEMHFLPDIYVSCDVCHGKRYNRETLEIQYKGRNINEILQMTVENAHTFFE
                     SVPVVARKLQTLLNVGLGYITLGQSATTLSGGEAQRVKLSLELSKRDTGRTLYILDEP
                     TTGLHFQDIDLLLKVLHRLRDQGNTVVVIEHNLDVIKTADWIVDLGPEGGDGGGRIIV
                     EGTPESIVEHPESFTGLYLKPILAAQIKR"
     gene            7333..8604
                     /locus_tag="PK522_06610"
     CDS             7333..8604
                     /locus_tag="PK522_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNC41441.1"
                     /translation="MNPRELLLNSFKIALEAVDPLKLIPAHLPAPPKRNTLVVGAGKA
                     AAAMAQAVEIHWSPDANLEGIAITRYGHGLPTQRITVIEAGHPIPDQNGEMATRAIME
                     SARILGSDDLMLCLFSGGGSSLLSMPIEGVTFEELKDINRQLIRCGASIQEINTVRKH
                     LSNVLGGRLAAACQAPIHCLIISDVTGDNPTHVSSGPCSPDPTTYADALALIKHYAID
                     ISSNVSAILQQRSQNPADETPKAGDTAFRNVKSRVIATAHQSLVAVESFFREHKITPM
                     VLGDTVTGEAREVAKVYAALAREIRHYGTPFRPPVALISGGETTVTIKGQGRGGRNTE
                     FLLSLAIELNGLEQVYALACDTDGIDGTENNAGAILTPDTIARAQLLGLKPIDLLNNN
                     DSYQLFEWLNDLVVTGPTRTNVNDLRVILIT"
     gene            complement(8645..10453)
                     /locus_tag="PK522_06615"
     CDS             complement(8645..10453)
                     /locus_tag="PK522_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC41442.1"
                     /translation="MWSDPAIAYLDKARSSTNHDIARRMRFGGLFYLLCAISIVLFSP
                     DLREQSSIIAFIVCFFILALLRLFLFKQVELNTFSDALIERLIFTVYVLTALSWVGFF
                     IIILATINEISDGVLIAIAVTIGFLSGGISATAPRMALMTTFASLIFLPCLFSLAIFL
                     PIDSGWTVLFVGLAYFIYCVHSGKLQNHDYWIIRQQASLLETQTKDLEQARSQAEQAN
                     KAKSAFLAAMSHEIRTPMNGVLGMAELMANTPLNAVQSQQLAIIRSSGRTLLRVIDDI
                     LDFAKIEAQKLAIVNRAFSPSHLIQEIKLLFRPKADETGLDFIIDIDTPLPTLLTGDP
                     DRIKQVLFNLLGNAFKFTPAGSVQLSVKYIDIPESDIVELHFTIIDTGIGISHEDQKL
                     LFQDFRQVGESTQHIQGTGLGLAITQNLLTLMGGTIALTSESGRGSQFKVCIPLKHAT
                     EVNSQVESKTVEPINSVKLKSTDRRLKVLLVEDNVVNQMISRAMLEHLNCEIAIASDG
                     KAAIAEYQNQHPDLILMDCNMPIMDGFEATRHIRRLEQQTNQPRIPIIAVTAHAFDHI
                     RQDCLDAGMDEHLSKPFDLNQLAMLLNQFRNSPASK"
BASE COUNT         3082 a         2233 c         2446 g         2977 t
ORIGIN      
        1 gaggaaattt catgaatagt gattcaattg ctgtacgcaa agctgatgcc agtagcgcaa
       61 atgccttgca agtgaatatt cggatgcaac caggtgagca taccggacag cctatttttt
      121 ccaatctaac atcggtacag gcgagccaag gtgtggttgt gatcgatttt ggatttatcg
      181 agccacaaat gattcaagcg ttaagtcgat tggctcaatc tggcgaaaaa gcacctgata
      241 cgattagcgc aaagatgtca tgtcgtatgg tggttagcgt tgaggcagct agccagttag
      301 ctcagcagct tagccagctt tttgtaaaaa aaacatgaaa atgtaaactg accaggagtg
      361 attgtgaacc aatctaatcc gctaccagtg aagattaaga tacagcagga cgagcggtca
      421 ggaaagccca gttatgctaa ttttacgact gtgcaacatg gccaaggcaa gattgttatt
      481 gattttggtt tttttgatcc aaagttgtta caagtatctg tcaatgttga taacaagcca
      541 ttaggtgtaa tcgatgctca cttgtctcgt cgtattatcc tcagtcaaga ggtagcaacc
      601 cagtttgctc aacaattgag ccaactgttt acaaagcacg gagatgtaaa agaaccacaa
      661 agtgaatcgg tgatgacgga tactctaccg agtgaaatac aggaccacag tcctgaacaa
      721 ggtgagaatt tatcagaaaa tcccaagaag gggttccgct ttccttggtt caagaaaaaa
      781 tagtaaaaca ggaagggcga tgacttccag tgcttatctg gttatttacg agtattttta
      841 ttaaaagaag cgtggtgttt ggtgggtgct ggattccggt tctcaatatg gcggaacgta
      901 attcgtccat tgctgatatc gtacggtgaa agttctatgg taattttgtc tccagccagt
      961 atacgaatat gattcttttt cattttaccc gcagtgtagg ctacaagctg gtgcccattt
     1021 tctaacgtga ctaaataccg agaatcaggt aataccttat caacggtgcc attcatttca
     1081 ataagctctt cttttgccat caatttattt cctggaaagg tgttgattaa aaataaaaag
     1141 cctggaatgt gtcccaggct tttatagcgg gtcacacaat ttagtgaccc ataaagaagt
     1201 tggtgatcgt acttaagcag gacgtatatt tactgcctgc ggacctctag gtcccggcga
     1261 agtatcatag cttactcgtt gattttctct gagtgttcta aaaccgccac cttgaattgc
     1321 tgagtaatgt acaaatacat cagtactacc gttttccggt gtgatgaaac caaatccttt
     1381 ttcgtcattg aaccacttaa cagtgcccat tgccatgttt tatatccttt aaagaaagtt
     1441 attaagaaga aagggactta ttccccaaag gcgaggatca agatgacaaa ggaaagaggg
     1501 ataaaaaaac caacaacatt tatatcataa gaaaatttaa accaaacagc tttaacattg
     1561 cttgagaatc gctttccata atatgcgcct tattgatgag ataagcaaat ttattttagg
     1621 caatagtcga tcttcataat ggtttgtcag gatagcgttt aataaccagc agcaactgct
     1681 tgttcaagcc agttagaaaa tgtgccaatc gagataatct gattacctga agcagggcct
     1741 aagtagtaaa cctcaccatt cgctataccc acataagatt gggtagtcgg gtaatagcga
     1801 tagtaataat tttcaaacgt tgctgagaca gtttgacctg agccgagtgg attggccggg
     1861 gatagatatt gcgaataaaa tgcttctaaa taagaaaata aacgatctga atcattctcc
     1921 ggtgtatcgg ttttgttaga ggtgatccgg ctaacaatga ctagattttt ttcctgcaat
     1981 ttgtgatctt caatcgtacc gatgatgggt aatgtcgaaa aaacacttcc tgcattggtt
     2041 cggggtaatg cagcaatggc atcagccact tgcatgccat tacccagcac ttgtccaaat
     2101 actgtaaatc cttcattttg cttattcaga tctgcagagt tatcggcgag attgataaac
     2161 cattcgctgg tagcgctatt gggtttgtca ggcagctttg ccatagcaat cgtaccgcgc
     2221 acattggaac gcgtgacact aaattcattg tcgactggtg atagtttggc aatagtattg
     2281 gccgcattcg tcgtttgatt ccatacgtag cctccccctt gaatgatgaa tccaggtatc
     2341 gtgcgatgaa agaaagaacg atcgtaggcg ttgctcttta ggtattcaaa gaaattagca
     2401 actgttctag gtgcggtctc atcgtggagt ttaatatcga tgacccctag tgttgtttgc
     2461 agtcgaatga ttgtagctgt gcttgtaagt ggggaaatta agagaaaaag accgactaat
     2521 cctggccaaa gtcgagttaa tcgcaaatta ttcattattc taatgctcct cttggcttat
     2581 gctaaaaagg gatatcatct tccaaatcat caaatcgatc actttttttg gattgtccag
     2641 ttggtttatc tgaaaagtta gcgctattat attcgccgtc tttgtcagta ggttcgtaac
     2701 tagcaccacc ggaacggctt cctagcatct gcatacgatc agcaacgatt tcggtcgtat
     2761 agcgttctat attggattta tctgtccatt tgcgcgtttg taatctacct tcgatataaa
     2821 cttgtgagcc tttttttaaa tactcaccag caatctcggc ctgcttgcca aacatgacga
     2881 cacgatgcca ctctgttttt tcttgtttct ctccttcttt atctttccaa gtatccgtgg
     2941 ttgcaatgtt cagatttgcc attgcatcac cactcggcat atatcggatt tcagggtcac
     3001 gccctagatt tccaaccaag atcactttat tcactgatgc catgtttatg cgcctccttt
     3061 tatcaattga accacttttt cttcatcaaa accttttagg tctactttca aataagcaac
     3121 ttgttcatta gctaaaacga gtgcctcatg aacaccgggt aacgctgcga gttcttttga
     3181 taatccattt gctcgctgat tatccatttc tgcaacatga tacattcttg agcgaaccgc
     3241 agcaggcgat ttcattgtaa aggcgaaata aagccaaacc aataacaaca ttgtacaaaa
     3301 aatgataatc gctgtgagac caacgtgctg atataagtat ccaccaaggc ttgcacccac
     3361 aaatgcgcct aagaattgtg tgctactgta aataccgata gcggttcctt tggcttcgac
     3421 gggtgcaatt ttagaaataa gtgaaggcaa gctggcttct aataaattaa atgctgtaaa
     3481 aaaaatgact aatgcgatac cgattcccca taaagattga cttgtaaatg caatcaagcc
     3541 ttgacctatt gttagcaaag caattgcagt gatgaagacg ggttttagct tagctttttt
     3601 ttctccataa ataatagcag gtaccattaa taagacggat agaacaagaa ccggcagata
     3661 catttgccaa tgatgatcgg cagcaagccc ggcctcacga agtgaaagag gaacaacaag
     3721 ccaaagcgcc attaaaaccg catgtaaagc aaaaatacca taatttaatc tgagcaattc
     3781 tggatgacgg agcgctgtcg agaaacgact tttcgagact tcagcatcgg aatggaagcg
     3841 gctgacgatg ggatcgggaa tgattttatg aaccacgata atggcgatta tcgcaagtaa
     3901 gcccgtcata atgaaaatac ccggtacacc aatccattga ttgagcaatg gcgcaatcat
     3961 gagtgaaatt gcaaacgttg caccaattgt catgccaatc attgccatgg cttttgttcg
     4021 gtgttcttca cgtgtaagat cggccgcaag cgccatgact gctgcggaaa ttgcgccagc
     4081 accttggatt acacggcctg cgattaccca ataaatgtca acggcggttg cagcgataaa
     4141 gctaccgatc gcaaataaca cgagaccaag ataaataaca ggtttacgac cgatacgatc
     4201 ggataaccag ccaaatggaa tttgcaaaat agcttgagtt aatccataag cgcccagtgc
     4261 gataccaact agcgtaaaat tatctccacc cggtaaatgt tcagcaaata atgcaaaaac
     4321 cggtaggata atgaataggc caaacatacg cagaccgtaa acgctggcaa gaccgaccga
     4381 agcgcgcttt tcgagcggtg acattttctc agaattgggt aacgacgaca tgaatctgat
     4441 tgtgataaaa ctagaaagtt gggtatatta tcaggttgac ccatacttag atagccgatg
     4501 gaactaatca aaatacgagg ggcgcgtacc cacaatctca aaaatatcaa tctggatatt
     4561 ccccgcaata agcttgtcgt cattacgggt ttatcagggt caggtaagtc ttcactggct
     4621 tttgatacac tttatgcgga agggcaaaga cgttatgtcg agtcgttatc cgcttatgcc
     4681 cggcaatttc ttcaattaat ggagaaaccc gatgttgatt taatcgaggg gctgtcacct
     4741 gcgattgcca tagaacaaaa agcaacctcg cataatcctc gatcaactgt cggaacggta
     4801 acagaaattc atgattactt gcgcttacta tacgcacgag ttggcgatcc tttttgtcct
     4861 gaacatggaa ttaaactctc tgcccagagc gtgtcgcaaa tggttgatca tgtcctgcga
     4921 ttgccagctg atactaaact aatggtacta gcgcccagtg ttgtgggacg taaaggcgag
     4981 caacaggatc tgtttgatga attacgtgcc caaggattta caagaatccg actagatgga
     5041 tcgatttacg agattgatca gcttcctaag cttacaaaaa ataaaaagca tacgattgag
     5101 gtggtaatcg atcggttaaa agtttcttcg gaagcaaagc agcggttagc agaatcgttt
     5161 gagatggcat tacgccatgg cgatgggcgg gcaattgcag tcgagatgga aacagggcaa
     5221 gagcatcttt tttcagctaa atttagttgc cctgtctgta attatgcgct atcagaactc
     5281 gagcctcgac tcttttcttt taataatccc gtaggagctt gtccaaaatg cgatggactg
     5341 ggacaaatta cgttcttcga tcccgcacgt gttgtgacat taccgcattt atcgttggca
     5401 gcaggtgcta ttaagcattg ggatcggcgc aatcaattct attttcaaat gttgactgcg
     5461 atcgcgaaac actatgaatt tgatattgaa acaccatttg aacaattggc ggacactatc
     5521 aaacagatca ttttgtttgg ctcgggaaaa gaaaagattc aatttacgtt tctcaatgaa
     5581 agtggtcgcg catccaaaga actttatccc ttcgaagggg tgattcctaa tttatcgcgg
     5641 cgttataaag aaactgattc tcagacagtt cgggaagaat tagctaagtt cattaattca
     5701 cgtatttgcc cagattgtga gggcacgcgg ctgtgtcgcg aagctcgcca tataagggtg
     5761 agtgagcaag caatctatca aattagcgct tggcccctga aacaagcaag tgcttttttt
     5821 gatcaattag tgttagcggg ccataagcag gaaattgccg aacgcattat taaagagatt
     5881 tccaatcgct tgcaatttct gaacaatgtt ggattagatt atttgtcttt agatcgatcg
     5941 gctgatacgt tatcaggcgg tgaagcccaa cgaattcggt tggcaagtca gattggatca
     6001 gggttgacag gtgtgatgta tgtgctcgat gaaccttcga ttggcttgca tcagcgcgat
     6061 aatggacgat tgctcgatac cttgcaacag ctacgtaatt tagggaatag cgttatcgtt
     6121 gttgagcacg atcaagacgc catttttcta gcagaccatg tggtagatat ggggcctggg
     6181 gcaggggaac atggtggttg tatcattgcg cagggtgccc ccagcgaaat tcaacatcat
     6241 ccagcttcac taacaggtca atatctctct gggaaacgat caatcgcgat tccagctgtg
     6301 cgaacccccc ttaatccaga gcggatacta acgctatctg gagtaaccgg aaataatttg
     6361 aaacaggttg aacttaatct tccagtaggg ttgctggtat gcatcaccgg tgtatcggga
     6421 tcaggaaaat ccacattgat caatgacaca ctataccgag cggttgctca gcagttgtat
     6481 ggcaataaca cggaacccgc accctatcaa actttggaag gcatcgcttt tttcgataaa
     6541 gtgatcaaca tcgaccaaac cccaattggt cgtacgccgc gttccaatcc ggctacctat
     6601 acaggtttat ttacacccat tcgtgaattg tttgccggcg ttcctcaagc ccgagaacgt
     6661 ggctatacac ccggtcgttt ttcgtttaat gtgaagggag gacggtgtga atcctgtcaa
     6721 ggggatggtg tcattaaggt agaaatgcac tttctgccgg atatttatgt ttcgtgtgat
     6781 gtgtgccatg gcaaacgcta taaccgcgaa acgttagaga ttcaatacaa ggggcggaat
     6841 atcaatgaaa tcttacaaat gacggtagaa aatgcccata ccttttttga gtcagttccc
     6901 gtcgtggctc gtaaattgca aacattattg aatgtcgggt tgggttatat tacgctggga
     6961 caatcagcga ctaccttgtc tgggggggaa gcgcaacggg ttaagctttc attggaattg
     7021 tctaagcggg atacggggcg taccttatat attctggatg agccaaccac agggctgcat
     7081 tttcaagaca ttgatctcct tttgaaagta ctgcatcgtt tgcgtgatca gggaaatact
     7141 gtagtggtga ttgagcataa tttggatgtg atcaaaacag cagattggat tgtcgatctt
     7201 ggtcccgaag gaggggatgg gggtggtcga attatcgttg aaggcacacc ggaaagcatt
     7261 gttgaacatc ccgaaagctt tacggggctt tacttgaagc cgatactcgc agcacagata
     7321 aaaaggtaag gtatgaatcc acgcgaactc ctattaaata gctttaagat tgcgctcgaa
     7381 gcggttgatc ctttgaagct catacccgcc catttaccgg cacctcccaa aagaaatacc
     7441 ttagtcgttg gagctggtaa agctgctgca gcgatggcac aagcggttga aatacattgg
     7501 tcacctgatg ctaatttaga aggtatcgct attactcgct acggacatgg tttaccgaca
     7561 caacgcatca ctgtgatcga agctggtcac cccattcctg atcagaatgg cgaaatggcg
     7621 acacgggcga tcatggaatc tgcccgaata cttggttccg atgatttaat gctttgttta
     7681 ttcagtggag ggggctcgag tttactctcg atgccgatcg aaggtgttac ctttgaagaa
     7741 cttaaagata tcaatcgtca gttaattcgg tgtggtgcgt caatacaaga aattaatacc
     7801 gttcgtaagc atctatcaaa tgtattggga ggaagactcg ctgcagcctg tcaagcgccg
     7861 attcattgtt tgatcatttc agacgtgacg ggagacaatc caacgcatgt ttcatccggg
     7921 ccgtgctcgc cggatccgac cacctacgcg gatgcgcttg cattgattaa acactatgcg
     7981 atcgatattt catccaatgt aagcgcaatt ctgcaacaac gaagtcagaa cccagctgat
     8041 gaaacgccta aagcgggtga taccgcattt agaaatgtga agagccgtgt catcgcaacc
     8101 gcgcatcaat cgttggttgc agttgaaagc ttttttcggg agcataaaat aacgccgatg
     8161 gtattgggtg atacagtcac cggagaagcg cgcgaagtcg ctaaggttta cgcagcactt
     8221 gcaagagaaa ttcgccatta tggtacgccc ttcagaccac ctgttgcatt gatatcagga
     8281 ggagaaacga ccgtaaccat taaaggacaa gggcgtggtg ggcgcaatac ggagtttcta
     8341 ctatcccttg caatcgagct caatgggtta gagcaagttt atgcactggc ttgtgatact
     8401 gatggaattg atggtactga aaacaatgcc ggtgcgatct taacaccgga taccattgcc
     8461 agagcacagt tgctcggtct caaaccgata gacttactca acaataacga ttcgtatcaa
     8521 ttattcgaat ggttgaacga cttggttgtg acgggaccga cacgaaccaa tgtcaatgac
     8581 cttcgggtga ttttgattac atgaaaacct ttacaatagg cgctagccat gacacagatt
     8641 atgattattt gctagcagga ctattacgaa attgattgag tagcattgct aactgattga
     8701 ggtcaaatgg tttgctgaga tgctcgtcca taccagcgtc gaggcaatct tgccggatat
     8761 gatcgaatgc gtgtgcagtc accgcaataa tgggaatacg cggttggtta gtttgctgct
     8821 ccagtctgcg aatatggcgt gtagcctcga atccgtccat gatcggcatg ttacagtcca
     8881 tcaatattaa atccgggtgt tgattctggt attcagcaat cgctgctttt ccatcgctag
     8941 caattgcaat ttcacaattc agatgctcga gcattgctcg gctgatcatc tgattcacca
     9001 cgttatcttc gaccagtaat actttgaggc gtcgatctgt acttttcagt tttactgaat
     9061 tgattggttc gaccgttttg ctttcaactt gcgagttaac ttctgtcgcg tgttttagag
     9121 gaatacaaac cttaaattgc gaacccctgc cactttcgct tgtgagtgcg atcgttccac
     9181 ccattaacgt tagtaaattt tgggtgatag caagacctag gccggtacct tgaatatgtt
     9241 gtgtcgattc tcccacttgc ctaaagtctt gaaatagcag tttctggtct tcgtgtgaaa
     9301 tcccgattcc cgtatcgatg atcgtaaaat gcaactcaac gatatcagac tcaggaatat
     9361 caatatattt gactgaaagc tggacactac ctgcgggtgt aaatttaaaa gcattaccga
     9421 gcaaattaaa taagacttgc ttgatgcgat cgggatcacc agtgagtaag gtaggtaatg
     9481 gggtatcaat gtcgatgata aaatctagcc ctgtttcatc tgctttagga cgaaacagta
     9541 atttgatttc ttggatcaaa tgcgatggac taaatgcgcg attgacgata gcgagctttt
     9601 gggcttcgat tttagcgaaa tcaagaatat catcgatgac acgtaatagc gtgcgccctg
     9661 agctccggat aattgccagt tgctgggatt gcacagcgtt aagcggcgta ttggccatta
     9721 gttccgccat tcctagcaca ccattcatcg gcgtgcgaat ctcgtgactc attgcagcta
     9781 aaaatgccga tttcgcctta tttgcttgct cagcttgtga gcgagcttgt tcaagatctt
     9841 tcgtttgtgt ttccagtagt gatgcttgct ggcggataat ccaataatca tggttttgta
     9901 gctttccact gtgaacgcaa taaatgaaat aggctaaacc cacgaatagg actgtccagc
     9961 cggaatcaat gggtaagaaa attgcaaggc taaagaggca aggcaaaaag atcaaacttg
    10021 caaatgttgt cattagcgcc attcgtgggg ctgtagcaga gatgccgcca gacaaaaaac
    10081 caatcgttac tgcgatcgca ataagcaccc catcgctaat ctcgttgatt gttgccagaa
    10141 tgatgataaa gaaaccaacc caactaagcg cggtaagaac gtacactgta aaaataaggc
    10201 gctcaatcaa tgcgtctgag aatgtgttaa gctcaacctg cttaaataag aatagacgga
    10261 gcagtgcaag gataaagaag catacgatga aagcaataat gctcgattgc tcacgcaaat
    10321 cgggtgagaa aagtacaatg ctgattgcgc acagtaaata aaataaccct ccaaagcgca
    10381 tgcgccgggc tatatcgtgg ttcgtgctag accgtgcttt gtcgagatag gcgatggcag
    10441 gatcggacca cataccaatg ttgcagtgaa ataattaaga agaatcggat aacactaggc
    10501 gattcgtcga tattgaatga cttcggcgat atatttttta ttcattttat tgtctcccga
    10561 tcaatcaact atagtgcgta ccattttcaa aagcgatggt atgcgcttgc ctataagttt
    10621 aactgattga tcgcttgctt caataaattc ttactagttg tatcaaggac gcagaggtga
    10681 ccggtttctt ttacattcaa actgatatca gggggttcat catgtcaatg caatcgat
//
LOCUS       DAOQLJ010000075        10513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2441_length_10513_cov_5.708548, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000075 DAOQLJ010000000
VERSION     DAOQLJ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10513
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2441_length_10513_cov_5.708548"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1124
                     /locus_tag="PK522_06620"
     CDS             <1..>1124
                     /locus_tag="PK522_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41443.1"
                     /translation="AAYSKTYSSESVATLQGVLAGVTVDMDTAAEIDAYLTSIGFTPG
                     VLLTEGTDIVTGHVFIAPRGFTPGGTDQVNTLNDDDRLTGTSATDDRLEFDFVNDADT
                     GDNNIAPTMNGIEIANIKFATDADATLDLQDSTGFTHINLFRIDDENLAEIDNIQEAT
                     TNNLSINDSNSPFSDVEFTYLGTALAGTTDAVTLTMNNAQVNDVFIAENRNTPAEGFE
                     TVNVVSSGAANSMVALSTEDVQTLNISGDQDLSIFGTEDIISVVGDNTLIEATATTGG
                     LLRMAGSLTKIDASTLTGDLKLNLNDVLQATQDGTSGTDVAVSVIGGTGDDTFWLGNG
                     IGSNDTIDGGDGGNIAILTGGSIDGNINKTGKLEVRTNTT"
     assembly_gap    1125..1224
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1225..2766
                     /locus_tag="PK522_06625"
     CDS             <1225..2766
                     /locus_tag="PK522_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41444.1"
                     /translation="NDSESNSVEMGGTLPETLLTITGGEAGDFINFDIDTAGADVTNV
                     ITGVEADNGEVQQGLLGIDTDGSATDFEAGNIFDVEGLATQVRQVAATIDASEELANV
                     IIRVSTNENSVDGAQSITMGKGDDTVIFDMLNDSRAGLTISDTVAGGDGNDTLVIDGH
                     DTRVSLGASEWTNVTGFETIRLVGNNAAPVSTLIGQNSYNLTLTNDLIDANGGDMIHI
                     INDNDVNNDEEDEVDTATTGTEFGVTIDARTLNAQNHFSYNGEEGASRTTDKFILGDA
                     NVNGANVIDGGAVDNDGDTTFAANTDILEVRNTATVTTGDLANVRNVGIIAGSNDQAT
                     EQTLVLQLNDTVIDAMVDSYHASSTTEVETLSVRLNDAADVSAVAGMGIDLDTTGTTA
                     KSAITVDLDTVVGGTDVFKIGYGLLTVNNFTTGTDTIQLSVSKFGLTLDADDIGLVAN
                     TDTDADGTNIEIGVAAATAADTRIVIDTATGDIFYDADGNGAQSQVLIAQINGIAAGD
                     VFIVA"
     gene            2887..4425
                     /locus_tag="PK522_06630"
     CDS             2887..4425
                     /locus_tag="PK522_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HNC41445.1"
                     /translation="METWTGGYVADVNYIYGYYPELNPMRAQLALCGAGIVVPKIEVA
                     CELGFGQGLSINLHAAASDVNWYGTDFNPAQAAFAQQLAQQAKSQAHLFDQSFEAFCQ
                     RLDLPDFDYIGLHGIWSWISNKNRAVLVDFIQRKLKVGGVLYVSYNTYPGWAATVPLR
                     ELMLQHTEFMGSEQQGVIGKIDAAIEFAETLFAVNPRFASDNPQVGVRLKNQNRNYLA
                     HEYFNRDWQPMSFAQISQWLAEAKVQYAGSAHYQDYIDTINLTKAQQELLARLPSGRF
                     YQTVRDFCVNQQFRRDYWIKGARKLSPLEKREQLYEIAVVLTQLRSGFEPKIVGSLSE
                     AKIQPTIFNPLLDCLADNKPKQLGQLVQALKNQKITAAQVLQAALLLSGSGALQCAQG
                     EAAIQSARKKTDALNQYLMQKARSNAEINYLASPVTGGGISVPRFHQLFLLGYQQRQQ
                     PKSISAGELAQFAWRILSSQGQRLTKAGKPIETAEANLAELKSIAEKFLGNDLSVYQA
                     LQII"
     gene            complement(4422..5267)
                     /locus_tag="PK522_06635"
     CDS             complement(4422..5267)
                     /locus_tag="PK522_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNC41446.1"
                     /translation="MATTILSPNNPMTITLICIGLGFGTGIILALTGAGGTIIAVPLL
                     VLSLNLSVVEAVPIGLLAVSLAALIGASIALAQGNVRYRAAGYLAIMGSIVSPLGIIA
                     AQHLPNMVLTVLFAILLTYIAWRHFKISSAPRTSLSERFSVPCLLNDGSNRLVWNAPC
                     FRALTFSGLIAGFLSGLLGVGGGFIINPALKKISNLSMQTILGTTLAVVALISMVGVI
                     SAMLISTLQWSIAIPFVSSTIVGMLFGRYLARYVTESRSQQLFASLAACVAIVMLYKA
                     ISSVL"
     gene            complement(5242..5595)
                     /locus_tag="PK522_06640"
     CDS             complement(5242..5595)
                     /locus_tag="PK522_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNC41447.1"
                     /translation="MFKESLTETHPTALSAMLSNRAAAGQACALLKVLANEDRLLILC
                     QLIEGEKNVGELEQALGIRQPTLSQQLTVLRDENLVETERKGKFIYYRLTSQEALQIM
                     KMLYQLFCGHHHTES"
     gene            complement(5645..6079)
                     /locus_tag="PK522_06645"
     CDS             complement(5645..6079)
                     /locus_tag="PK522_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HNC41448.1"
                     /translation="MKTLTHLIALLAGLIFGFGLIVSDMVNPDKVQDFLDLSGSWDPS
                     LAFVMGGAIAVGFLAFTFAKRRNQTFMGTPIHLPTTHLIDKRLVLGSLAFGIGWGMAG
                     ICPGPGLVLVGSGSVQGMVFVAAMLIGMGIFELLERRKQKKP"
     gene            complement(6099..6530)
                     /locus_tag="PK522_06650"
     CDS             complement(6099..6530)
                     /locus_tag="PK522_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HNC41449.1"
                     /translation="MNIDWTSFTPVSALIGGGIIGIATAVLLLFAGRIAGISGIVGGL
                     FKQPSADTGWRIAFLAGLLLAPICWQQFSNLPPIQINANDATLALAGFIVGIGTRYGS
                     GCTSGHGVCGISRLSPRSIVATMLFIATGVMTVYFIRHLIS"
     gene            6626..7486
                     /locus_tag="PK522_06655"
     CDS             6626..7486
                     /locus_tag="PK522_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011113024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC41450.1"
                     /translation="MRAQIRAFFDPATWTMSYVVYDEQGGFCAIIDPVLDYDPKSGRT
                     KTVSADKLIDFVRENRLTVEWLLETHAHADHLSAAAYLQDKVGGKTAIGESIALVQGS
                     FKKLFNLGPEFVADGLHFDHRITDGEVLRVGKITLKAISVPGHTPADMAYQLDDTVFV
                     GDTLFMPDVGTARADFPGGDAHQLYHSIQKILSLPPQTRLFMCHDYPPSNRSPQWEST
                     VLEEKMHNIHIKDGVTEDAFVAMRTKRDATLEMPVLMLPSVQVNIRAGRFPDPESNGV
                     SYLKIPINAI"
     gene            7517..9160
                     /locus_tag="PK522_06660"
     CDS             7517..9160
                     /locus_tag="PK522_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNC41451.1"
                     /translation="MKRFDHARRQFLQAATFGSLVSLWPSLLLAGESQKSTTITSTFK
                     PDVEIELFARNDKVSMMSGAATDVYRYHGQLLQGPSTTLSTMPSYLGPTLNLVQGQKV
                     RIHFHNQLAEPCITHWHGMHVPQRMDGHPMYEVAEGGTYIYEFEVKNPAGTNWYHAHT
                     HELTAKQVYRGLAGLIVISDEQEQQLQLPSGEYDLPIVIQDRRFTESNQLYYRQHGMH
                     ERMVGFLGDTILVNGQINPTMPVKTRAYRLRVFNGSNSRIYKLAWEDGTPLIAIGTDG
                     ALLEKPESYPYIMLAPAERVELWVDFSGREVGSELVLQSLAFHGAAPSMGMRGRMGGG
                     MEGEAMGMMSGLAQGEQFPIAKFRITEKMSESPQLPQTLIKMNRLTARDASNPDRTIP
                     IAISMAHMSWKLNGRSFEMGNVMDIEKIPVNTIQKIRIFHESHQMHGGRGGMGGGMRG
                     GRGGGMGMTMSMAHPIHLHGQQFQILSRQLTDQQATGGYDTVKQGFINSGWKDTVLVM
                     PGEEVTIIKPFEDYTGLYLYHCHNLEHEDLGMMRNFLVS"
     gene            9306..10241
                     /locus_tag="PK522_06665"
     CDS             9306..10241
                     /locus_tag="PK522_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNC41452.1"
                     /translation="MNVFFKSQFNCFGLCLVSLLLWTSISFANSEADFFKATLTGDGA
                     TVEKLLAEGLDINLKNPDGFTALAVASQKGHDAIVKLLLDKNAEVDAANIQGGTALLL
                     AVKNGHAAIVDTLLAKGADPNLQAKNGLTPLIIAAAKDYADIVKSLVAKGADPDLSDT
                     SQTTALHIAAQNGHVDIVNTLLTNGAKPDLQDVNGASALILAALVGAQEVVQLLLEKG
                     AKADLKATNDFTALILAAQNGQTAVVETLLARGASINQQNQDGITALMWAALHDHIDT
                     VKLLIEKGADTKLKSKSDKTALDMAKDQTIIELLK"
BASE COUNT         2987 a         2092 c         2537 g         2797 t
ORIGIN      
        1 ctgctgcata ttcgaaaact tactcgagcg aatcagtcgc aacattgcaa ggcgttctcg
       61 ctggcgtaac ggttgatatg gatacggctg cagaaatcga cgcttattta actagcatcg
      121 gctttactcc tggtgtgtta ctgaccgaag gtacggatat tgttacgggt catgtcttta
      181 ttgcgccaag aggatttacc ccaggtggaa ccgatcaagt caatacactg aatgatgatg
      241 atcgtttgac gggtacatct gcaacggatg atagacttga gtttgatttt gtgaacgatg
      301 cggatacggg cgacaataac atcgcaccaa ccatgaacgg cattgaaatc gctaatatca
      361 aatttgcaac agatgcggat gcaacgctcg atttgcaaga ttcaacgggg tttacacata
      421 ttaatctgtt cagaatcgat gacgaaaatc ttgcagaaat tgacaatatt caagaagcaa
      481 caacaaataa tctgtcaatc aatgactcaa actcaccttt ttctgatgtt gagtttactt
      541 acttgggtac agcgctagcc ggcacaaccg atgctgttac gctaaccatg aataatgcgc
      601 aagttaatga tgttttcatt gctgagaatc gcaatacgcc agccgaagga ttcgaaactg
      661 tcaatgttgt gtcaagtggc gcagctaact caatggttgc tctgtcgacc gaagacgttc
      721 aaacattgaa tatctcgggc gatcaggatt tatcgatttt tggtacagag gatattatct
      781 ctgtagtcgg ggataatacc ttgattgaag cgacagcaac aaccggcggg ttgctcagaa
      841 tggcgggttc attaaccaag atcgatgctt ctacgctgac aggtgatctg aaacttaatt
      901 tgaatgatgt tttacaagca acacaagatg gaacatcagg aaccgatgtg gctgtatcag
      961 ttatcggtgg aacaggagac gatacattct ggttaggtaa tggtatcgga tcaaacgata
     1021 cgatcgatgg tggagatggc ggtaacatag ctattctcac agggggttca atcgatggca
     1081 atatcaataa aacaggtaag cttgaagtac gcaccaatac aacannnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnaacgat tcagagtcta attctgtaga aatgggcggt
     1261 acattgcctg aaacgctatt gacaattacc ggtggtgaag cgggtgattt catcaacttt
     1321 gacattgata ccgctggtgc agatgtaaca aacgtgatta ctggtgttga agcggataat
     1381 ggtgaagttc agcaaggttt gctgggaatt gatactgatg gttctgcaac tgacttcgaa
     1441 gccggtaata tttttgatgt agaaggttta gcaactcaag ttcgtcaagt tgctgcgacc
     1501 attgatgctt ctgaagaact ggctaatgtg atcattcgtg tcagcaccaa tgaaaacagt
     1561 gttgacggcg cacaaagcat taccatgggc aaaggcgatg ataccgtaat ctttgacatg
     1621 ttgaacgatt cacgtgccgg actgacgatt tctgacaccg ttgcaggggg tgatggtaac
     1681 gataccttag tgatcgatgg ccatgacact cgtgtatcac tcggtgcatc agaatggacc
     1741 aatgtgaccg gttttgaaac cattcgtttg gtgggtaaca atgctgcgcc agttagcacc
     1801 ttgattggcc aaaacagcta taacctcacg ttgaccaatg atctaatcga tgccaacggt
     1861 ggtgatatga ttcatatcat caatgataac gatgttaaca atgatgaaga agatgaagtg
     1921 gataccgcta caacgggtac cgaattcggt gtcactatcg atgcacgtac actgaatgca
     1981 caaaaccact tctcctataa cggtgaagaa ggtgcgagca gaaccacaga taaatttatc
     2041 ttgggtgatg cgaatgttaa tggtgccaat gtaatcgatg gtggtgcagt agataatgat
     2101 ggtgacacta cctttgctgc taatactgac attctcgaag tacgtaacac cgcaactgta
     2161 acaacaggcg atttggcaaa tgtcagaaac gttggaatca ttgctggctc aaatgaccaa
     2221 gcgactgaac aaacactagt actgcaattg aacgatacgg ttatcgacgc tatggttgat
     2281 agttatcatg cttcatcaac cactgaagtg gaaactttat ctgttcgttt gaacgatgca
     2341 gctgatgtat ctgctgttgc tggaatgggc atcgatctgg atacaaccgg aacaacggct
     2401 aagtctgcta ttactgttga tcttgataca gttgttggtg gaaccgatgt attcaagatt
     2461 ggttatggtc tgctaaccgt aaacaacttt acaaccggta ccgataccat tcaattgtca
     2521 gtcagcaaat ttggattgac attggatgcg gatgatatcg gtttagttgc taatactgat
     2581 actgatgctg acggtaccaa tatcgagatt ggcgttgcag cagcaacagc agcagataca
     2641 cgtatcgtga ttgataccgc aactggtgat atcttctacg atgcagacgg taatggtgca
     2701 caatctcaag tgctgattgc tcaaatcaat ggtattgctg caggcgatgt ctttatcgta
     2761 gcttaataac cgtagtaacc gaagttaagt gtgcaacagt aatgttgctc actaagtaga
     2821 aattaaaaaa accgcccgta tttagggcgg tttttttttg atcggatttt aaatgaatgg
     2881 cttgatatgg aaacttggac tggcggttat gtagcggatg ttaattatat ttatggctat
     2941 taccctgagc taaatccaat gcgagcacag ctcgctttgt gcggcgcagg catagttgtg
     3001 cctaaaatag aagtggcttg tgagctcggt tttgggcaag gccttagcat caatctgcat
     3061 gcggctgcat ctgacgttaa ctggtacggc acagatttta acccagctca agcagcgttt
     3121 gcacaacaat tggcgcaaca agccaagtca caagcacatt tatttgatca atcatttgaa
     3181 gcattttgtc agcggttaga tttacctgat tttgactata ttggcttgca tggtatttgg
     3241 agctggattt caaacaagaa tcgtgccgtt ctcgttgatt ttattcaacg taaactcaag
     3301 gttggcggcg ttctatatgt aagctacaac acctatcctg gatgggctgc taccgtacct
     3361 ttgcgagagc tgatgttgca acatactgaa ttcatgggat cagagcagca aggtgttatt
     3421 gggaaaatcg atgctgccat tgaatttgct gaaacgctat ttgctgtaaa tccacgattt
     3481 gcaagcgata atccacaggt gggcgtccgc ctgaaaaacc aaaaccgtaa ctatctggca
     3541 cacgaatatt tcaaccggga ctggcagccg atgtcttttg cacaaatttc gcagtggctt
     3601 gctgaggcaa aagtgcaata tgctggttct gcccattatc aagattatat tgacacgatc
     3661 aatttgacga aagcacagca ggagttgctt gctcgcttgc cgagtgggcg cttttatcaa
     3721 accgtacgtg atttctgcgt caatcaacaa tttcgcagag attactggat taagggagct
     3781 cgaaaattaa gtcctttgga aaagagagag cagctctatg agattgcagt tgttttaacg
     3841 caactgcgtt cagggtttga acccaaaata gtgggaagct tgagcgaggc gaagattcaa
     3901 cctactattt ttaatcctct gctggattgt ttggctgata ataagcccaa gcaacttgga
     3961 caattggttc aggcgcttaa aaaccaaaag attacagcag cgcaggtttt gcaagccgca
     4021 ttgctgttga gtggtagtgg ggctttacag tgtgcgcagg gcgaggcagc aattcagagt
     4081 gcccgaaaaa aaactgatgc tttgaatcag tatttgatgc aaaaagcacg tagtaatgct
     4141 gaaattaatt acctggcgag tcctgtcacg ggcgggggta tttcagtgcc gcgctttcac
     4201 cagcttttct tactgggtta ccaacaacgc cagcaaccta agtcgatttc tgcaggggaa
     4261 ttagcacaat ttgcctggcg gatattgtct tcgcagggtc aacgacttac caaagcaggt
     4321 aagccaatcg aaacagcgga agcgaattta gctgaattaa aatctatcgc tgaaaaattt
     4381 ctgggtaatg atttatcggt ttatcaggct ttgcaaatta tttagagcac actggagatt
     4441 gctttataca gcattacgat tgccacgcaa gcagcgagtg aagcaaatag ttgttgagac
     4501 cgtgattcag tgacatatcg cgcgagatat cgaccgaata acattcctac tatggtactg
     4561 ctaacgaatg ggattgcgat cgaccattgt agcgtgctga ttagcatcgc tgaaatcaca
     4621 ccgaccatgg aaatgagtgc aacgacggct aacgttgtgc cgagtatcgt ttgcatcgat
     4681 aagttagaaa tttttttgag cgcagggttg atgatgaatc ctccccctac ccctaataag
     4741 cccgacaaaa aacctgcgat caatccagaa aatgtgagcg cacgaaagca aggcgcattc
     4801 caaactaagc gattcgagcc atcattgagt aggcaaggta cggaaaatcg ctcagagagc
     4861 gatgttcgtg gcgctgaact tattttaaaa tgacgccatg caatgtaagt gagtagtatt
     4921 gcaaacaaaa cggtgagcac catattgggt aaatgctgtg cagctatgat gccgagcggc
     4981 gatacgatag aacccataat ggcaaggtaa cctgctgctc gataacgaac atttccttgt
     5041 gcaagcgcga tcgatgcacc gatcaatgcc gctaaactaa ccgccaataa accaattgga
     5101 actgcttcta caacggatag attgagactt aatactaaaa gcggtactgc gataatggtg
     5161 ccaccagcac cggtaagcgc aagaatgata ccggttccaa agcctaatcc aatgcaaatg
     5221 agtgtaatgg tcattgggtt gttaggactc agtatggtgg tggccacaaa ataactgata
     5281 gagcattttc atgatttgta atgcttcctg gcttgtcaat cggtaataaa taaatttacc
     5341 tttacgttcc gtttcgacta gattttcatc gcgtaataca gtcagttgct gtgacaaagt
     5401 cggttgtcgg ataccaagtg cttgctctaa ttcaccaacg tttttttctc cctcgattag
     5461 ctggcaaaga atcagtaagc gatcttcatt ggcgagcact tttaataatg cacaggcctg
     5521 gcccgcagct gctcgattgg atagcattgc tgatagtgcg gttgggtgag tttcggtgag
     5581 tgattcctta aacatagttt ggtgaaagag ggttgaatat aatggtggtg gttgtttctt
     5641 aattttaagg tttcttttgt ttacggcgct ctagcaattc aaatatcccc ataccgatta
     5701 acatggcagc aacaaatacc ataccttgca cgcttcctga gccgacgaga accaacccgg
     5761 gacctgggca aatacctgcc ataccccagc cgataccgaa agcaagactg ccaagtacta
     5821 atcgtttatc gatgaggtgt gtggttggca aatgaatcgg tgtacccatg aaggtttggt
     5881 tgcgtcgttt ggcaaaagta aaagccagaa atcctaccgc aatggcaccc cccatcacga
     5941 aagctaatga aggatcccat gagccactca gatccaggaa atcctgaact ttatcgggat
     6001 taaccatatc cgatacaatc aaaccaaatc caaagattag gccagccagt agcgcgatca
     6061 aatgagtaag cgttttcatc gtgttcagtt gagttgaatt aactgatgag atgacggatg
     6121 aaataaaccg tcatgacacc cgttgcaata aaaagcatgg ttgcgacgat cgatcgaggt
     6181 gaaagccggg agattccaca aaccccatga ccactcgtgc aaccggaacc ataacgtgtt
     6241 ccgataccga cgatgaatcc tgcaagtgcg agtgttgcat catttgcatt gatttgaatc
     6301 ggaggcaagt ttgagaattg ttgccaacag atgggggcaa ggagaagtcc tgctaaaaag
     6361 gcaatgcgcc agcctgtatc ggcgctgggt tgtttgaata atccgccaac aatgcctgag
     6421 attccagcga ttcgacctgc aaacaggagt aacactgcag ttgcgatacc gatgatgccg
     6481 ccaccgatta aagcagatac aggtgtaaag ctcgtccaat caatattcat cgaaatcccc
     6541 aaaatttatc tttacatata atcttttagt atattatatt aatatatata ttatagtcaa
     6601 ctaatatgaa ataaggttaa gcataatgcg tgcacagatt agagcgtttt ttgatccagc
     6661 gacgtggacg atgagttacg ttgtttatga tgagcagggt ggattttgtg ccattatcga
     6721 tccagtgctg gattatgatc cgaagtcagg aagaaccaag acggtatcag ctgataagct
     6781 gattgacttc gttcgggaaa accgattaac ggttgaatgg ctccttgaga cccatgctca
     6841 tgcagaccat ctttcagcag ccgcttatct gcaagataaa gtcggtggaa aaacggcgat
     6901 tggtgagtct attgcccttg tgcaaggtag ctttaagaag ctgtttaatt tgggtccaga
     6961 atttgtagca gatggtttgc acttcgatca ccggatcacc gatggtgaag tgcttcgcgt
     7021 aggaaaaata acgcttaaag caatatcggt tccaggtcat acgcccgcgg atatggcgta
     7081 tcagctcgat gacacagtat ttgtaggaga taccttgttc atgccggatg ttggaactgc
     7141 acgtgctgat tttccgggtg gtgatgcgca tcagttgtac cattcaattc agaaaattct
     7201 gagtctacca ccgcagaccc ggttatttat gtgccatgat tacccaccca gcaatcgttc
     7261 tccgcagtgg gaaagtacag tgcttgagga gaaaatgcac aatattcata tcaaagatgg
     7321 cgtgacggag gatgcgtttg tcgcgatgcg aacgaagcgt gatgcaacat tagaaatgcc
     7381 agtgctgatg ctaccttcgg tgcaagttaa tatcagggca ggccggtttc ctgatcctga
     7441 gtcgaatggg gtttcctatt taaaaatacc gattaatgca atttaatttg cgtagttaaa
     7501 aaaagaggaa cagaaaatga aacgttttga tcatgccaga aggcaattct tacaggcggc
     7561 tacctttggc tcattggttt ccttgtggcc aagtttactg cttgctggag aaagccagaa
     7621 aagcacaacg attaccagta cctttaagcc tgacgtagag atcgaattgt tcgcgcgcaa
     7681 tgacaaggtt tcgatgatgt caggtgccgc aacagatgtt tatcgctatc atgggcagtt
     7741 actgcaaggg ccttccacga cgttgtcaac gatgccaagt tatcttggcc caacgctaaa
     7801 tttggttcaa ggccagaaag tgcggattca ttttcataat cagctagctg agccctgcat
     7861 cacccattgg catggcatgc atgtaccaca gcgcatggat ggccacccaa tgtatgaggt
     7921 tgcggagggt ggaacctata tttatgaatt cgaggtcaag aatcctgcag gaaccaactg
     7981 gtatcatgca cacacacacg agttgacggc gaaacaagtt tatcgtggtc ttgcgggact
     8041 gattgttatt agtgatgagc aagagcagca attgcaattg ccaagcggcg aatatgatct
     8101 accgattgtg attcaagatc ggcgtttcac agaaagcaat caactctact atcgccaaca
     8161 tggcatgcac gagcgcatgg taggattctt gggggatacg attttagtta atgggcagat
     8221 taatcctact atgccagtaa aaacgcgggc ctatcgattg agagtattta atggctctaa
     8281 ttcaaggatt tataaattgg cttgggagga tggcacaccg ctcattgcta ttggaaccga
     8341 tggtgcgttg ttagaaaagc cggaaagcta tccttatatc atgttggccc ctgctgaacg
     8401 tgttgaatta tgggtggatt tttcaggacg tgaggtgggt tctgagcttg tgttgcagag
     8461 cttagccttc catggggcag ccccatcaat ggggatgcgt ggaagaatgg gaggcggtat
     8521 ggaaggcgaa gcaatgggca tgatgagtgg tctggcacag ggcgaacaat ttccgatcgc
     8581 taaattccgc atcactgaga aaatgagtga atcacctcaa ttgccgcaaa ctttgatcaa
     8641 aatgaatcga ctgacagcgc gcgatgcatc aaatccagat cgaacgattc cgattgcgat
     8701 tagcatggcg catatgtcgt ggaaactcaa cggacgttca ttcgaaatgg ggaatgtaat
     8761 ggatattgaa aaaatcccag tcaataccat tcaaaaaatt agaatttttc atgagagtca
     8821 tcagatgcat ggagggcgtg gtggtatggg tggtgggatg cgaggcggtc gaggaggagg
     8881 tatgggcatg acgatgtcga tggcgcaccc gattcacctt catggtcagc aatttcaaat
     8941 cctctcccgg caattaacag accaacaggc aactggtggc tacgatacgg tcaaacaggg
     9001 ctttattaat agcggctgga aggatacggt attggtgatg cctggcgagg aggttacaat
     9061 tattaagccg tttgaagact atacgggtct gtatctctac cattgccata accttgagca
     9121 tgaagatctc gggatgatgc ggaatttcct cgtgagctga aggtgaacac taaactgtgg
     9181 gcgccattga gcttggcgtt gattgcacag taaacgcttt tacaaacgaa gcgttaggaa
     9241 aagtgaggta tacttttcca ctttatttaa ccttataagt taatttctag cacatgctac
     9301 tcattatgaa cgtatttttt aaatctcaat tcaattgctt tggattatgc ttagtttctc
     9361 tcttgctttg gacttcaatc agttttgcaa atagcgaggc agattttttt aaggcaacgc
     9421 taactggcga tggtgcaacc gtagaaaaat tattggctga aggcttagac attaatttga
     9481 aaaacccaga tggatttacg gcgttggctg tggcttctca gaaagggcat gacgccatag
     9541 tgaaattact actggataag aatgctgaag tggatgcggc gaatattcaa gggggaaccg
     9601 cgttgttact cgctgtaaaa aatggccatg ctgcgatcgt cgatactcta cttgcaaagg
     9661 gcgcagatcc caatttgcag gctaaaaatg gattaacccc actcatcatc gcagccgcaa
     9721 aggattatgc tgatattgtt aaaagtttgg tggcaaaagg agcggatcct gacttaagcg
     9781 atactagtca aacgacggcg ctgcatattg cagcgcagaa cggccatgtt gacattgtaa
     9841 acacgttgct tacgaatggt gcaaaacccg atcttcaaga tgtgaatggc gcttcagccc
     9901 ttattttagc agctttggtc ggtgctcaag aggtggttca actgctactg gaaaaaggtg
     9961 cgaaagctga cttgaaagcg accaatgatt ttacagcgct tatcttggcg gcacagaatg
    10021 gtcagactgc agttgttgaa acgttgttag cgcgaggagc gagtataaat cagcaaaatc
    10081 aagatggaat tacggcattg atgtgggctg cgttacacga tcatatcgac actgttaaat
    10141 tgttgattga gaagggcgct gataccaaac ttaaaagtaa aagcgataaa actgccttag
    10201 atatggcaaa agatcaaacg atcattgaat tgctgaagta agtgaagaat aaagtttatg
    10261 gtgctaatgt gatgtgaatt ttgcaaaagt aagcgaaaat attctataat agcaagctaa
    10321 ctatggcagt ttcatagtta tatcccatcg ttcctgacct tccttcaatt ttctttcttt
    10381 ggaaattgct tatacccctg gttagcagcg atgcattaat cctgcgctag cggtattggt
    10441 tgactcgttc aattcattcc tgcttgcttt tttactcaaa cttaatttgg gaaataacgt
    10501 gtcttttgaa aat
//
LOCUS       DAOQLJ010000076        10411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2471_length_10411_cov_5.833140, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000076 DAOQLJ010000000
VERSION     DAOQLJ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10411
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2471_length_10411_cov_5.833140"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..174)
                     /locus_tag="PK522_06670"
     CDS             complement(10..174)
                     /locus_tag="PK522_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41453.1"
                     /translation="MCLIDTCRDRGIWHIVIRIRYIVFHVAWNNLRVKLPAWREHVME
                     MNKVGPWPEN"
     gene            536..1180
                     /locus_tag="PK522_06675"
     CDS             536..1180
                     /locus_tag="PK522_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41454.1"
                     /translation="MRMQIVLLAASLGFATVSLAKPSFQLDEIDRDGNTLIIKFSGNR
                     SGNIVQDIENIHAWYNGKELGVGLPHGLYNYFWQPISGRWGADGAQISFDGRANNFLF
                     TDLSFTPINLPAGQFLTNGDYVYDVSKFFGNALPLPQDDPAAYSEVTVRYPTLGTPST
                     SFAFDYHDNVTYSLSEIQIPVPGSISLIACGVLGLFLLHRRSRDKRNNLIRMIF"
     gene            complement(1497..1793)
                     /locus_tag="PK522_06680"
     CDS             complement(1497..1793)
                     /locus_tag="PK522_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNC41455.1"
                     /translation="MTYKLEFKKSALKEWEKLGHTIKDQFKKKLKERLKNPHVPSAAL
                     SGAENIYKIKLRQLGYRLVYSVEDKTVTVTVIAAGKRDRNEIYDIALSRLREKA"
     gene            complement(1783..2031)
                     /locus_tag="PK522_06685"
     CDS             complement(1783..2031)
                     /locus_tag="PK522_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002057569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNC41456.1"
                     /translation="MTHQILTEIAASISELKANPMKVVSSGNGMPIAVLNRNEPAFYC
                     VPAAAYEAMMELIDDTELLKLVKERIDEPTVTVSLDDL"
     gene            2434..3297
                     /locus_tag="PK522_06690"
     CDS             2434..3297
                     /locus_tag="PK522_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNC41457.1"
                     /translation="MTPEVIQTIAAYLAMAVPATVAISNIAKLIMEWLQQRHKIVEAQ
                     IQLSHQITTHYLDRALDPAAPLAIRHQLLRFLATPDLQGARLSTWAKAELERVGGIVD
                     ETNRAVASAEEGLRLAKNAADLERAEQKLVEAVRRQRGLLEPPTTPPVTAAALRAGLI
                     QEKKLNRLEMPGADLTNTKLVYRELRGANLKGANLSHSSLQGCDLRATDLSGALLIGT
                     TLYTADLRGANLTNSKLRSCNLQQARLEGADLRGAEIADCDLRATYDDSTQWPDAFDP
                     VQSGAVHTVSG"
     gene            3387..3731
                     /locus_tag="PK522_06695"
     CDS             3387..3731
                     /locus_tag="PK522_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41458.1"
                     /translation="MSIIEGYCSPISVAGGQNISFHISSQLNFRLEFVCLSEAETEKM
                     IIVGGIQNIFPAGVYPTPLRANETGCNWPSLLLITIPTFWNSGTSMELQWGQTRLFAR
                     NKSQLSKIQRAM"
     gene            3965..4315
                     /locus_tag="PK522_06700"
     CDS             3965..4315
                     /locus_tag="PK522_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41459.1"
                     /translation="MNFVEMSEQEIIEIANPIMDNLMDASTRIDHEAHVRDFTDRIKK
                     IVTKEHFQLICKQYQNEKGFFAERKLVAVFKRPNSAAIVWKQYFTKAKGEFVAEMVLV
                     HQNGKFFCDHAMVF"
     gene            4581..5114
                     /locus_tag="PK522_06705"
     CDS             4581..5114
                     /locus_tag="PK522_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNC41460.1"
                     /translation="MIEVIILAIALSMDAFAVSIGLGAKKNGPNMALRAGGFFGIFQA
                     LMPFIGYWGGQSVLGWVETYAHWIAFGLLTLIGAKMIYESTQEGVEETIARITNKMLL
                     VLAIATSIDAMAAGFSLTLLEVNPYVACLVIGVITFVFSVVGVYIGNRSGTWLESKAE
                     IFGGIVLILMGIKFLIL"
     gene            5370..5855
                     /locus_tag="PK522_06710"
     CDS             5370..5855
                     /locus_tag="PK522_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC41461.1"
                     /translation="MTTPAMNTICLWYNGNAQDAAQFYADTFPNSFIGAVYRAPGDFP
                     SGKEGDILTIEFTLMGIPCIGLNGGPAFVHNEAFSFQVATTDQAETDRYWNAIVDNGG
                     QESECGWCKDRWGVSWQITPIALMKAITNPDPAAAKRAFDAMMLMKKIDIAAIEAALR
                     G"
     gene            complement(5990..6946)
                     /locus_tag="PK522_06715"
     CDS             complement(5990..6946)
                     /locus_tag="PK522_06715"
                     /inference="COORDINATES: protein motif:HMM:NF023695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HNC41462.1"
                     /translation="MKATQQLTRHYAGISNPSGAVALSDNLFIVGDDEDNRLRIYDRD
                     TFDKPLHILSLSDIFKGAIKDGPDLEIDIESMAAMSNVLFIIGSHSTSRTGEYRPSRH
                     RLMAIQFKLDASNKVDIHPVGEIYTHLIDDLQQDARFKPYRLDKAAKTPPKAIGGLSI
                     EGLAATPDNGLLIGFRNPLSGGKIRNERLVKGNALLVKLKNPFEVIHGLPARFAKPIE
                     LDLEGQGIREITWRKKHQFMIVAGPYHENIASKTHSKETGKLYHWFSRSKKLKEYKKV
                     ALENLNIEAAFFYPKDNKTVQLLSDDGQMRNEKRFRSQIVKL"
     gene            complement(6954..8774)
                     /locus_tag="PK522_06720"
     CDS             complement(6954..8774)
                     /locus_tag="PK522_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41463.1"
                     /translation="MPYLTIKALFIFLIKTLIYAGAVYYTTNQFTIDNLGTVILGVFI
                     LSLPIILCGTYGSTVRQIRRLTIFKERGILFKIVSSRFLKSILWILWALSTSFFMLLQ
                     FHTYSEWEWFFFLLVIPFFWIVFSTCRKFIAHEIVGYMATDIALRWARFLCPILMLIL
                     YYAFFGQFETLPNYANIPEAIQDQKSRVENLAGSTITREASHFLALYDGIRIFILNHS
                     LSQDTIWGFLLLTFSKLIVFFNACAILSCFVIPTTEFRRIVGPLTDQDIPPALSIQRI
                     AIASAIFTLLTLFIYAPTFVYLEAWVQQKSIALNEDRTKIEKVAVDLIGEEPFRKGTI
                     EKLHKAKIKALPDKAAIQRDLDEKLEIGFKRLESRVDIYLDWYYSLMGEYTRISKLLI
                     GELEEDMKRRFQEALEQGGNPFWEYEAAVSQLLMHYEDAQKAYIEKANKILHKNRLDL
                     KGVPFDVNEQLTLLDAVNLPIHQEDFINLQHRLGTGSAAGMITAIIVGKVAGKSIFKL
                     AVGALFKVVASKATGAVVSAGAGAATGAAVGSVVPGLGTAIGAVIGGIIGGIGAGIIV
                     DKTLIELEAAISREKFKAEILAAINEARLEFKEKLINPAE"
     gene            complement(8913..8997)
                     /locus_tag="PK522_06725"
     tRNA            complement(8913..8997)
                     /locus_tag="PK522_06725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8961..8963),aa:Leu,seq:gag)
     gene            complement(9063..9431)
                     /gene="secG"
                     /locus_tag="PK522_06730"
     CDS             complement(9063..9431)
                     /gene="secG"
                     /locus_tag="PK522_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNC41464.1"
                     /translation="METLILAVHVIVALMIIVLVMLQQGKGADMGAAFGSGSSGSLFG
                     ASGSANFLSRATAVAATVFFITSLSLAYFSGSGVKSDSVMKRYEAGTIESSESQDMTD
                     AIPSATEKVDDGSKAMQIPE"
     gene            complement(9451..10206)
                     /gene="tpiA"
                     /locus_tag="PK522_06735"
     CDS             complement(9451..10206)
                     /gene="tpiA"
                     /locus_tag="PK522_06735"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNC41465.1"
                     /translation="MRGKLVVGNWKMNGNMLQNQQLLDAIIPELKAFTNMTSAVCVPY
                     PYLTMTQKALQATKITWGAQNVSHYDLGAYTGEVAAEMLVDLGCYYVIVGHSERRALF
                     GEDNQTVALKFKKAQSVGLVPILCVGETLAQREIGETERVIAEQLDTVVNLVGIEALA
                     KAVIAYEPVWAIGTGKTATPDQAQSVHAFIRNRLASHNAELASKIQILYGGSVKSNNA
                     VDLFSMPDIDGGLIGGASLIAGDFISICKAAQG"
BASE COUNT         2890 a         2327 c         2235 g         2959 t
ORIGIN      
        1 ctagctgctt tagttctctg gccaaggtcc aaccttattc atttccatga catgctcgcg
       61 ccaagcagga agcttcactc tgagattatt ccaggcaaca tgaaaaacaa tgtatctgat
      121 acgaatgacg atgtgccata tccctctgtc tcggcacgta tcaattagac acattcgcta
      181 aaagaacggg tatccgatat tgagtgctgc ttgtattgca gtggagtcct gaaaatcatc
      241 gccaccatct tgaaaagagc agcaatcaaa agattcttga ctatttcggc ttatcctcca
      301 gagtttcgct acgtcagtgc atttcctgaa tacgctgacg tctactcttg aacgccatta
      361 gcgtgaacgg caaaaaaagg gacaaaacca gcactttttg cctctgatta aagacaaatt
      421 ttgtgaattc cggatgattt tctggtcagt ttgacaaccc aatcccttac tagtagattt
      481 ggttcttata aaagaaaacg attatttttc ctatacgcca caaaggagac tttaaatgcg
      541 catgcaaata gtacttctag ccgcgtcact tggctttgca accgtatcct tggctaagcc
      601 gtcttttcag ctagatgaaa tagatagaga tggtaatacc ttaatcataa aattcagcgg
      661 caatcgctcc ggaaacattg tccaggatat agagaacata catgcttggt ataacggaaa
      721 agaacttgga gtcggactac cacacggcct ctataattat ttttggcagc ccatttccgg
      781 tcgatggggc gcagatggtg cacagatctc cttcgatggt agagccaata attttctttt
      841 caccgatcta tccttcacgc ccatcaattt acctgctggt caatttttaa ccaatggaga
      901 ttatgtttac gatgtaagca agttcttcgg caatgcactt cccttacccc aggatgaccc
      961 tgctgcttac tctgaagtaa cagtaaggta tccaacacta gggacgccta gtacatcatt
     1021 cgcttttgac tatcatgaca acgtaactta cagcctgtcg gaaatacaaa taccggtgcc
     1081 tggttccatt tctctgatcg cgtgcggggt gctcggacta tttctcttgc ataggagatc
     1141 tcgcgataaa aggaacaacc ttattcgcat gatattttaa ggttcaatta aaaattcctt
     1201 acgttgtttt tttgaactga gctaatagta cttgaacaaa tcagtattaa atattgaggc
     1261 gtaggaaatg ttttggcatc ggagtaccac ggaatcgatt gattcctctc aactgctctg
     1321 gttagtgagg tcagatgtaa ttagtgtcac taattgtata ctgactgatc attttccaag
     1381 gttaagggac gtctttcacg ctatacgatt tccaatcgat cgcagcataa tattcaatct
     1441 agaaagcatt actaagtgat ttttgtgaca gaaatgaatt acttcgatgt tatgactcat
     1501 gccttttcac gcaatcgcga gagggcgatg tcgtagattt cattccggtc gcgtttgcca
     1561 gctgcaatta cggtgactgt tacggtcttg tcttcaacgg aataaacaag gcgatagccg
     1621 agttgtctta atttaatctt gtagatgttt tccgcacccg acaatgcagc agatggaacg
     1681 tgaggattct tcaatcgttc ttttagtttc tttttgaatt gatctttgat ggtatggcct
     1741 agtttttccc attctttgag cgctgacttt ttaaattcca gcttataagt catccagtga
     1801 aactgtaacc gttggttcgt ctatacgctc ttttaccagt ttaagcagct ctgtatcgtc
     1861 aatcaactcc atcattgctt cataggcagc agcgggcaca cagtaaaagg cgggttcatt
     1921 acgattcaat acggcaatgg gcataccgtt gccactggaa acaactttca tggggttagc
     1981 cttaagttca gagatactcg ctgcgatttc tgtcagtatt tgatgtgtca tagctttatt
     2041 tttgagcagt caaacaataa gactgaatta taggtctcct aaaaggtctt gtaaagtttg
     2101 cgattataat gttttagatc attgaaatgt gattggaaaa atatcagaac taaccttaat
     2161 cgctacaagc taaaataatc ctaagatcta ggatcaatca gcattgctct atttttatcc
     2221 tgtgatggac tcggaagcgg ggggttgctt tctgtgtgat tattcattca atttgtggca
     2281 ctaaacaagt ctgataaaat gacagctaaa ttgacgatta gctgtgaata taaggggata
     2341 tcatgaacaa ctccatatta gatttcattc gcgacgtaat atactgcgtc atttcggacg
     2401 tctaattcta gttaggcgtc gaaaacaacg atcatgaccc ccgaagtaat tcagacaatt
     2461 gcggcatacc ttgcaatggc cgtaccggcg actgtcgcga tctccaatat tgcaaaactg
     2521 atcatggagt ggctgcaaca acgtcacaag atcgtcgagg cacagattca gctcagtcat
     2581 cagatcacga cgcactacct cgaccgcgct ctcgatccag ccgcgcccct tgcaatccgc
     2641 catcagcttc ttcgcttcct tgctacaccg gacttgcagg gcgctcgact aagcacgtgg
     2701 gccaaggccg aattggaacg ggtgggggga attgtcgacg agacgaatcg tgctgtggct
     2761 tcagcagaag aaggcctgcg tctcgcaaag aatgccgccg atctcgaaag agccgagcag
     2821 aagctggtag aagccgtacg gcgacaacgc ggtctacttg agccaccaac cacaccccca
     2881 gtcactgcag cagcgctgag ggctggattg atccaggaga agaagctcaa tagacttgag
     2941 atgccgggag ccgacctgac aaacaccaag ttggtgtacc gcgagcttcg aggagcgaac
     3001 ctaaaggggg ccaacctttc tcactccagc ctccaaggct gcgaccttcg cgccacggac
     3061 ctctcagggg cattactcat cggaacgacc ctgtacaccg cggatcttcg aggcgcgaac
     3121 ttgacgaact cgaagctgcg ctcctgcaat cttcagcagg cacgcctcga aggggctgat
     3181 ctacgcggcg ccgagattgc tgactgcgac cttcgggcca cttacgacga ctctacccag
     3241 tggccagatg cattcgatcc tgtgcagtcc ggagcagttc atactgtatc cggctaggca
     3301 tggagagtga accctgtcga cgcctaactc tacgtagggt ttactcaata catctcagtt
     3361 ttaaacttgc gatctaagga tacaacatgt caattataga agggtattgc agcccgatct
     3421 ctgtagctgg aggacagaac atttcatttc atatttcttc ccagttgaac tttcgactcg
     3481 aattcgtgtg tttatcggaa gctgagacag aaaaaatgat aattgttggt ggaatccaaa
     3541 atatatttcc tgctggtgtt tacccgactc cattacgcgc aaatgaaaca ggatgtaatt
     3601 ggccatcact attgttgatc actatcccta cattctggaa ttctggaact tcaatggaac
     3661 ttcaatgggg tcagactcga ttgtttgcga gaaataaaag tcagttaagt aaaatccaaa
     3721 gagctatgtg agatcaattt ctgcttttat cctttgatga acccagaagc caaggtttgc
     3781 ttttattgtg tagttatttt tttcaacttg tggcactaac aagtttgata aaacaacagc
     3841 taaattcgct attggcggta ataaggggct atcatgaata attccgtatt aaattacatt
     3901 tgtgatgaat atattgtgct atttcggatg cctgattgta tgttagcact tgcaaggagt
     3961 agtcatgaat tttgtagaaa tgtcagaaca ggagattata gaaatagcaa atcccatcat
     4021 ggacaatttg atggatgctt ctacaagaat tgatcacgag gctcacgtaa gagatttcac
     4081 ggatcgcatc aagaaaatag ttacgaaaga acatttccag ttgatttgca agcagtatca
     4141 aaatgaaaaa ggcttctttg cagagcgtaa gcttgtagca gtattcaagc gtccaaattc
     4201 tgcagcaatc gtttggaagc agtatttcac caaagctaaa ggcgaatttg ttgctgaaat
     4261 ggttcttgtt catcaaaatg ggaaattctt ttgcgaccat gcaatggtgt tttagcctac
     4321 gtgctaacta ggtgttcaac tggacagttt tcagtggggt aattgtttcg caaatctctc
     4381 tcgaaccata ggagatatga aattgggtac cgatgccgta ctccccccac aggaatttcg
     4441 ccatcataga ttttcatatc ggtattttca atgggcagag ttgccgctga gatcaatgtt
     4501 ctctgcgata tgatcaatgt tatgagcttc attattgaga gcataccata taaccacgta
     4561 cgttttttcg aagcaacacg atgatcgaag tcattattct tgcaatcgcg ctcagtatgg
     4621 atgcctttgc agtgtccatc ggtttgggtg cgaagaaaaa tggccctaac atggcactga
     4681 gagccggggg attctttgga atttttcaag ccttgatgcc cttcatcggg tattggggtg
     4741 gtcagagtgt tttaggctgg gtagaaacct atgcgcattg gatcgctttt ggactgctga
     4801 cgttgatcgg tgcaaaaatg atttatgaaa gcactcaaga aggtgtggaa gaaacgattg
     4861 cgcgcatcac caacaaaatg ctgctggttc tggccatcgc tactagtatt gatgcgatgg
     4921 ctgctggatt tagcctgact ttgttggaag ttaatcctta tgttgcttgc ttggtcatcg
     4981 gagtgataac ttttgtgttc agtgtagtcg gtgtttacat cggtaaccgc agcggaactt
     5041 ggctggaaag caaagcggaa atttttggtg ggatagtttt gattctgatg ggtatcaaat
     5101 tccttatttt atagtcagaa gggaccatca acagggccta gcctgtttta gtattacact
     5161 aattgcaagc tgatttctat tttgcaaaaa tatggcacat cttctccgtt tgatgctcat
     5221 caacgaatcg tggcatattg cttaacacaa gaataaccat cgatggtaat gaaatttggt
     5281 ctgcactgat ttgaacctca aatctttgat aagaagcaca ttaccgccct accaaaagat
     5341 tatatttttg atctcaatgg agaaatgaca tgacaacacc agcaatgaat acgatttgcc
     5401 tttggtataa tggcaacgcc caagacgcag cacagtttta cgccgacact tttcctaact
     5461 catttatcgg tgcagtgtat cgcgcaccgg gagattttcc gtcaggaaag gaaggagata
     5521 tattaactat cgagttcacc ctaatgggaa ttccatgcat tgggctcaac ggcggccccg
     5581 catttgtgca caatgaagca ttctcgtttc aggtagcaac tactgatcaa gctgaaactg
     5641 atcgctattg gaacgcgatc gtcgataacg gtggtcagga aagtgagtgt ggctggtgta
     5701 aggataggtg gggagtgtcc tggcagataa cgccaatcgc cctgatgaaa gctattacca
     5761 acccagatcc cgctgctgca aaacgggcat tcgacgcgat gatgttgatg aaaaaaatcg
     5821 atattgctgc aattgaagca gcacttcgtg gttgagatac ccgaaatcta acaatttcga
     5881 gcaagttgat tcttattttt cagcgtaact taacgtagat gttacgttac aggcaggatg
     5941 cctcagtttc cgcctttgct agaattttcc tgagatacaa agctttggat cacagcttaa
     6001 cgatctggct ccggaatctt ttttcattgc gcatttggcc atcgtcgctg agtaattgta
     6061 ctgtcttatt gtctttggga tagaaaaaag cagcttcgat attcaagttt tccaatgcta
     6121 ctttcttgta ttccttcagc ttcttcgatc ggctgaacca gtgatatagc ttgcccgttt
     6181 ccttggagtg tgttttactc gcgatgtttt catggtaagg ccctgcgaca atcatgaatt
     6241 gatgcttttt tctccaagtg atttcacgga taccttgtcc ttcgagatcc aattcgatgg
     6301 gtttagcaaa tcgcgctggt agcccgtgaa tgacttcgaa aggatttttt aatttaacga
     6361 gtaaggcatt gcctttcact aatcgttcgt tacgaatttt tcctccactc agtgggttcc
     6421 gaaagccgat caacaagcca ttatccggag ttgcagccaa cccttcgata cttaaaccgc
     6481 cgattgcttt cggcggtgtt tttgctgctt tgtctaatcg atatggcttg aaacgcgcat
     6541 cttgctgcaa atcatcgatc aaatgagtat agatttcgcc gaccggatgg atatcgactt
     6601 tattgctggc atcgagtttg aattgaatag ccatcagtcg gtgccttgag ggtcgatact
     6661 caccagtacg gcttgtcgaa tgtgaaccaa tgataaataa cacattgctc atcgcagcca
     6721 tactttcgat atcgatttct aaatcgggcc catctttaat ggcgcctttg aaaatatcgc
     6781 ttaacgataa aatgtgtaac ggcttatcga aagtatctcg atcatagatt ctgagtcgat
     6841 tatcttcatc atcgccgacg atgaaaaggt tatcgctcag cgctaccgca cccgagggat
     6901 ttgagatacc ggcgtaatgc ctagttaatt gctgcgttgc tttcatgggc agattattcc
     6961 gctgggttga ttaatttttc tttaaattct aaacgggctt cgttaattgc agctaagatt
     7021 tcagctttaa atttttccct actaatcgct gcttccaatt ctatcaaggt cttatcaacg
     7081 ataataccag cgccgatacc accgataatg ccaccaatga cggcaccaat tgcagttccc
     7141 aagccaggaa cgacggagcc aacagcagcg ccagtcgctg cccccgcccc agcactcacc
     7201 acggcaccgg tggctttact agcaaccact ttaaataacg cgcccactgc aagtttaaaa
     7261 atactttttc cagctacttt tccgacaatg atggcggtaa tcataccagc ggctgatcct
     7321 gtaccaaggc gatgctgaag attaataaaa tcttcttggt gaatgggtaa atttactgca
     7381 tccaacagcg tgagttgctc attgacatca aacggcacac ctttgagatc gaggcggttt
     7441 ttgtgcaaaa ttttgtttgc cttttcgata taggcttttt gagcatcttc ataatgcatc
     7501 agtaactgac taacagccgc ttcatattcc cagaaaggat taccaccttg ttccagtgct
     7561 tcttgaaatc ggcgtttcat atcttcttct aattcaccaa tcaatagctt actgatccgc
     7621 gtgtactcac ccatcaagct atagtaccaa tctagataga tgtctactct ggattccagc
     7681 cgtttaaaac cgatttctag tttctcgtct aggtcgcgtt gaatcgctgc tttatccggc
     7741 aacgccttga ttttagcttt atgcagtttt tcgatggtgc ccttgcgaaa tggctcttca
     7801 ccaatgagat cgacagcgac tttctcgatt tttgtcctat cttcattcag tgcaatcgat
     7861 ttctgttgca cccatgcttc caaatacaca aaagtgggcg cataaataaa tagagtcagt
     7921 agggtaaaaa tggccgaggc tatcgcgatt cgttggattg acagcgcggg gggaatatct
     7981 tggtcagtaa gcggcccaac gatgcgtcga aattccgtcg tcggaatcac gaaacaagaa
     8041 agaatagcgc aagcattgaa gaaaacaatg agtttgctaa atgttagcaa cagaaacccc
     8101 caaatcgtgt cttgtgaaag cgaatggttg aggataaaaa tccggatgcc atcataaagc
     8161 gctaagaaat gcgaagcttc gcgggtaata gtgcttcccg ctagattctc caccctgctt
     8221 ttttgatcct gaatcgcttc aggaatattc gcgtaattgg gtaacgtttc gaactgtcca
     8281 aagaaagcat aataaaggat tagcatcaat atcgggcaaa ggaacctagc ccagcgtaaa
     8341 gcaatatcgg ttgccatgta gcccacaatt tcgtgagcaa tgaacttgcg gcaagtgcta
     8401 aatacgatcc aaaagaacgg aatgaccagc aaaaaaaaga accattccca ctcgctatag
     8461 gtatgaaact gcagcaacat gaaaaaagaa gtactcagtg cccacagtat ccataaaatc
     8521 gatttcaaaa agcgcgacga aacgatctta aataaaatcc ccctctcctt gaaaattgtg
     8581 agacgcctaa tctggcgcac agtactgcca taggtcccgc acagaataat gggcaagctg
     8641 agaataaaga caccgagaat aaccgtccca agattgtcaa tcgtgaattg attggtagtg
     8701 taatagacag cacctgcgta gatcagggtc ttgatcagaa aaatgaaaag cgctttgatg
     8761 gttaggtagg gcatgaccaa gcgattgaag tttttattat tattctaccg attcagatcg
     8821 gatcgagacg ataagccgtt tattttgggt atcgcatgtg atactgattt tttaacaatc
     8881 agtacgagaa taatatagca gaaaaaaacg attggtgccg atggcgagac tcgaactcgc
     8941 acagctttcg ccaccgcccc ctcaagacgg cgtgtctacc aattccacca catcggcaat
     9001 tgaaaagtaa gcagaggata ggtgcttact ttcagaaaac atgattaatt aagcaatcat
     9061 tcttattctg gaatttgcat tgctttagat ccatcgtcga ctttctcggt cgcagaaggt
     9121 atcgcatcgg tcatatcttg gctttcacta ctttcgatgg tgcccgcctc atagcgcttc
     9181 ataacgctat cacttttgac accgctaccc gaaaaatacg ctaatgaaag gcttgtaata
     9241 aaaaaaacgg ttgctgcaac cgctgttgct cgactcaaaa aattagctga cccacttgcg
     9301 ccaaataaac tgccggagga accacttcca aatgcagcgc ccatgtcagc gcctttgcct
     9361 tgctgtagca taaccaagac aatgatcatc aatgctacaa tcacatgaac agctaaaatc
     9421 aacgtttcca taaactctat tctcttaaat tcagccctgg gctgctttac aaattgaaat
     9481 aaaatcacct gcaatcaatg aagcaccgcc aatcaatccg ccatctatat caggcatcga
     9541 aaataaatcg accgcattgt ttgattttac actaccgcca tataatatct ggattttaga
     9601 agctaattca gcgttgtgcg aagctaatcg attacgaatg aaagcatgca cactttgcgc
     9661 ttgatcggga gtcgctgttt ttccagtacc gatggcccaa actggttcat aggcgatgac
     9721 tgctttggca agcgcctcaa tacccactaa attgacgact gtatctaatt gctcagcaat
     9781 aacacgctca gtttcaccaa tctcacgttg agccaatgtt tcaccgacac ataagatggg
     9841 aaccaatcca acgctttgtg cttttttgaa ttttagcgct acggtttgat tatcttcccc
     9901 aaacaaggca cgacgttcag aatggccaac aatgacataa taacaaccca aatcgaccag
     9961 catttctgca gccacttcac ccgtataagc gccaagatca taatgactga cattttgggc
    10021 gccccaggtt attttagtag cttgtaacgc tttttgggtc attgtgagat agggataagg
    10081 cacacaaacg gcgctcgtca tgttcgtaaa cgctttcagt tcaggaataa tcgcatccag
    10141 taattgctga ttttgcagca tgttaccgtt cattttccag ttaccaacta ccaatttccc
    10201 gcgcattttt gcttaatcct atttccacat caaaaccagg tatggtactg ttgaaagcct
    10261 atcgcgtcaa ttactataca gttaggctgc ttttcttgac aaataagctg acatctttat
    10321 aatccactaa ctgtttcaat atatgcgctc ttgcttgcgg gaatagctca gttggtagag
    10381 cacaaccttg ccaaggttgg ggtcgcgagt t
//
LOCUS       DAOQLJ010000077        10179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2561_length_10179_cov_7.319637, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000077 DAOQLJ010000000
VERSION     DAOQLJ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10179
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2561_length_10179_cov_7.319637"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            438..1640
                     /locus_tag="PK522_06740"
     CDS             438..1640
                     /locus_tag="PK522_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HNC41466.1"
                     /translation="MSALIHLIYCSAATHPMNESELRKLLVKARAKNARLGITGILLY
                     ENGSFFQVLEGTPEVIDRLFNEICQDNRHNKVSTIIRESIFKRAFSEWTMGYSSVSLQ
                     ELDEIIGLNDFFTEGSSLTEITPGRAKKILAAFRDGRWRAKVQYTSSPESEAHTKQLI
                     MQPATMVTPKVSFSFQPMINAITGTVGAYEAILRGQHDEAFPEISQQIGDAEWTYFDT
                     NCRAIAISMAAKLGLPCDLHLSFVARRVEDAREAISASLDAAEKNNIEPSRIILQIDQ
                     DRLIGERDHFAQIIEEYRGAGLRISIDHFGLGQASFNLLELLRPEMISLNAQLVRDIN
                     NSGPRQAIVLGLKQTCTDLGINLVAKHIETVAELGWFVSEGIELLQGNVIASPAFERL
                     PNISWPLV"
     gene            1644..2360
                     /locus_tag="PK522_06745"
     CDS             1644..2360
                     /locus_tag="PK522_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC41467.1"
                     /translation="MESKVSFPQFSFAFQPVINISTGAIYAYEALARGCGNEPANHVF
                     RQVGKSNRYQFDEILRQKAISLAAKLGIGCNLSLNLLPQTLLYSQTALASSLAVAESV
                     GIPPELLTIEVMESELIDDICQFAKLINQYRGTGISLAIDDFGSGYSGLNLLADFQPD
                     SVKIDLSLIRSIDTRGPRQAIVRGIIRTCQDLGIDIIAEGIETPEEYLWCYEEGIELF
                     QGYYFAKPGFERLPVAFYPK"
     gene            2505..3116
                     /locus_tag="PK522_06750"
     CDS             2505..3116
                     /locus_tag="PK522_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41468.1"
                     /translation="MSGTLSIIGISAIRQIKARKDIFVASIPLIFALQQLTEGLLWVV
                     LQNPGLALAQASNWLAGLYGIFIGVIWPVYAPFAIYREETDSQTKRVMASMLLAGVGL
                     AAYTIIGMIQEPIVAGIINHSIHYAHEVEGQQFVLAMYLFATCTPFILSSRKLLNLTG
                     TIITLGFFVAFFFYQQTFASVWCFFAAIASGLIYFFIRNQTTK"
     gene            complement(3176..3670)
                     /locus_tag="PK522_06755"
     CDS             complement(3176..3670)
                     /locus_tag="PK522_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNC41469.1"
                     /translation="MPIKYSAVLIVFCCLVVGCSSVSQKERSPKQVRTPDSSIQTDSR
                     GVKGKLYAKYRQWEGVPYQWGGLSRSGIDCSGFVHVVYKRELGKIIPRTTELQARAGR
                     HVDINSLKAGDLVFFRIGNNTRHVGIYLENRRFMHASRSRGVTISQLDHRYWKSRYWK
                     SVRV"
     gene            complement(3753..4718)
                     /locus_tag="PK522_06760"
     CDS             complement(3753..4718)
                     /locus_tag="PK522_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HNC41470.1"
                     /translation="MRAVFLDFGSVTRGDLDCTKLENAISSWQYYDETSPQQIVERIK
                     TAEVVVSSKTFLDRAVIDSASHLKLICIAATGYNNVDLSAAAERNIGVCNVRNYATDS
                     VAQHVAMLMLNLVRRFVEYQSYIKHGKWQACAHFCSLDYGIEDLSGKILGIIGYGELG
                     RAVGKIGQAFGMQLRIAEHKGQPPRSGRLSFEEVLSESDFISLHCPLTAQTHHLITRT
                     ELALMKSTAYLINTARGKLINEADLLESLIDGRIAGAALDVLSEEPPTKGNILLTYPR
                     PNLIITPHIAWTSQAARQCLLDKIADNITHFFEGKPYNQVPEQPE"
     gene            4880..6130
                     /gene="glyA"
                     /locus_tag="PK522_06765"
     CDS             4880..6130
                     /gene="glyA"
                     /locus_tag="PK522_06765"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNC41471.1"
                     /translation="MFSKHNTIEQIDPDLWLAIKGEMQRQEDHIELIASENYASPAVL
                     EAQGTVLTNKYAEGYPGKRYYGGCKHVDIVEQLAIDRLKSLFNAEYVNVQPHSGSQAN
                     AAVYLSALKPGDTLLGMSLAHGGHLTHGASVNMSGKIFNSITYGLDPDTEEIDYKQVE
                     QLAKEHRPRMIVAGASSYARIIDWKQFRRIADEVGAYLFVDMAHYAGLIAAGFYPNPV
                     GIADFVTSTTHKTLRGPRGGVIMAKPEFEKALNSAVFPQTQGGPLMHVIAAKAVAFKE
                     AASQAFKDYQEQVLENARVMAKTLQNRGLRIVSGRTECHMFLVDLRAKNLTGKDAEAA
                     LEAAHITVNKNAIPNDPQKPFVTSGVRIGTPAITTRGFKEIETEKLANLVADVLEAPT
                     DTAVLNRVAKETQDLCAKLPVYER"
     gene            6181..6642
                     /gene="nrdR"
                     /locus_tag="PK522_06770"
     CDS             6181..6642
                     /gene="nrdR"
                     /locus_tag="PK522_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNC41472.1"
                     /translation="MRCPFCNAEDTSVINSRVSEEGDRVRRRRQCIVCGKRFTTYETI
                     ELRLPQIVKQNGNRTEFSRNKLLTGFLRALHKRPVPTEAVDAAIDRIIQHLLNSGARE
                     VSSRSIGELVMQELYNMDKVAYIRFASVYKSFQDVDDFQDVIKEVREIPKD"
     gene            6742..7845
                     /gene="ribD"
                     /locus_tag="PK522_06775"
     CDS             6742..7845
                     /gene="ribD"
                     /locus_tag="PK522_06775"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNC41473.1"
                     /translation="MPGEPSLFSAADYTFMAEAIRLAEKGLYTTSPNPRVGCVIVKNH
                     QIIGRGWHEKAGQPHAEINALKQAASEVKGSTVYVTLEPCCHYGRTPPCTDTLINAGI
                     AKLVIAAQDPNPRVAGKGIKQLKAAGIEVQYGLLEAQANQLNIGFVSRMTRQRPWITV
                     KIASSLDGKIALANGKSQWITGEAARTDVHRLRARSCAILTGINTVKQDDPELSVRYI
                     QTTRQPFRVILDNRLEIATNAKVLQKPGSLIFTTLQDNQRIDSLSEKGARVVVLSSST
                     GKLDLVEVMSTLAELEINELFVEAGRTLNGALIESRLVDEIIIYCAPIVLGNHAQSML
                     NLPELASLSQKWQLEIKEIGKVGSDIKITAKLI"
     gene            complement(7884..8384)
                     /locus_tag="PK522_06780"
     CDS             complement(7884..8384)
                     /locus_tag="PK522_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41474.1"
                     /translation="MIINQRLIVGTLFLVISASLNSLATAATENHDPLFVQTDGRMAL
                     QLSAGWNTFYSPFMYPQALLYPYPLFINPCYPYVHCGAFNQYRLLQERRKRSERSPSQ
                     HAKQRTFAFDRLALEYQRRGKLYRTNENEIVPAHKGDSKIKPEFRDSGSFLPTFLERH
                     NHSVKR"
     gene            complement(8457..>10179)
                     /locus_tag="PK522_06785"
     CDS             complement(8457..>10179)
                     /locus_tag="PK522_06785"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNC41475.1"
                     /translation="IPRGDRTQSVIEPMLTDQWYVAMEGMAKRGLDVVAQGEVKFVPE
                     NWTHTYQQWLENIQDWCISRQLWWGHRIPAWYDEVGNVTVAHDLEEAQRLSGKDQLIQ
                     DEDVLDTWFSSALWPFSTLGWPKETTELKTFLPTSVLVTGFDIIFFWVARMVMMSLHF
                     TGKVPFKEVYVTGLIRDAEGQKMSKSKGNVLDPLDLIDGIALSDLIKKRTTGLMNPKQ
                     AESIEKNTRKQFPQGIPAFGTDALRFTFASLASHGRDIKFDLQRCEGYRNFCNKLWNA
                     TRFVLMNCEGKAIGFDNTPHALNYSLADKWILSRLQQTLEAVEQGFNSYRFDMVAREI
                     YEFVWDEYCDWYVELAKTQLSSENEAIQRTTRYTLIKVLETALRLSHPIIPFITEELW
                     QKIGPLAGIKGGSIMVQSYPQSDSSAIDTEASDQIVLLKEMINACRTLRGEMKLSPAA
                     RVPLVIEGNAAVLNNFSSYISALAKLSEVTIISDGLPNDDAPVAVVRDFRLMLKIEID
                     VVAERERLSKEISRLEGEINKAQAKLDNADFIARAPEKVVAQEKERVAEFSAKKQKLT
                     EQLARLT"
BASE COUNT         2917 a         2257 c         2114 g         2891 t
ORIGIN      
        1 cctttcatca cctacctcat tatcctattg atcttgctat ataagatgaa aaacatctga
       61 gaatagttca taggcattaa attaaataga cctgatatca tgatcacatg atcacccaat
      121 cgatcgaaaa ttttataaaa caagggtata acgtgaaaat tgataggtgt ccccatatgc
      181 ttagcaatat taccccagtg gataaaattc gcatcttagt caaacgccag acgccaatca
      241 gtgagctcag cgttttctcc tatctcatag gccagttttg aaatgctttt caacaaaagc
      301 aaagtgagca accatggtct atttgataat taacctttcg cttattgctt ccttccacac
      361 tttgaactct gttgctgatt gtctagcatg ttagcgccat tacgcactat tttaaaacag
      421 gacattaagg atagccaatg agtgctttga tacacctgat ttattgtagc gccgcaactc
      481 acccaatgaa tgagtctgag ttacgcaagc tactggtaaa agcgagagca aaaaatgcac
      541 gactcggtat cacaggaatc cttctctatg aaaatggtag tttcttccaa gtattagaag
      601 gcacgccaga agttattgat cgactattta atgaaatttg ccaagataat cggcacaaca
      661 aagtgtctac cattattcgt gaatccattt tcaaacgtgc ttttagtgaa tggaccatgg
      721 gctattcgag tgtttctttg caagaactag atgaaattat tggccttaat gattttttca
      781 cggaaggctc cagccttaca gaaattacac cgggtcgcgc aaaaaaaatt ctagctgcgt
      841 ttcgagatgg acgttggaga gctaaagtac agtatacatc gagtccagaa tctgaagcgc
      901 ataccaaaca gttaatcatg cagccagcta ccatggttac tcccaaagtc tccttttctt
      961 ttcaacctat gattaatgca atcactggaa ccgtaggcgc ttacgaagcc attctgcgtg
     1021 gacagcatga tgaagccttt cctgaaattt cccaacaaat cggagatgct gagtggactt
     1081 atttcgacac gaattgtcgc gctattgcca ttagtatggc agctaaattg ggtttaccgt
     1141 gtgatttaca tctaagtttt gttgcacggc gagtcgagga tgcgcgtgaa gcaatttcgg
     1201 cctctctgga tgctgcagag aaaaataaca tcgaacccag ccgtattatc ttgcagattg
     1261 atcaagatag attgataggt gaacgtgatc attttgcgca aatcatcgag gaatatcgcg
     1321 gagcaggtct acgtatttcg attgatcatt ttggtttagg ccaggctagt tttaatttgt
     1381 tggaactgct gcgcccagaa atgatttcgt tgaatgcgca actagttcgg gatatcaaca
     1441 atagcggacc gcgccaagcc attgtgctag gtttgaaaca aacctgcact gatctgggta
     1501 tcaatctggt cgcaaaacat attgaaacgg tagctgaatt aggatggttt gttagcgaag
     1561 gcattgaatt attgcaaggc aatgttatcg ctagtccggc ctttgaacgc ttgcctaaca
     1621 taagctggcc tttggtgtag cgtatggaat ccaaagtgtc gtttccacaa ttctcgtttg
     1681 ccttccagcc tgttattaat atttcgaccg gggcaattta tgcgtatgaa gcattggcac
     1741 ggggttgcgg taatgagcca gccaaccacg tattccggca agtcggtaaa tctaatcggt
     1801 atcaatttga tgaaattttg cgacaaaagg cgatatcatt ggcggccaag ttgggtatcg
     1861 gttgtaatct gagtttgaat ttacttcccc aaacattatt gtattcgcaa acggcgctgg
     1921 catcatcctt ggcagttgct gaatcagtcg gtattcctcc tgaattgctc acaatcgaag
     1981 taatggaatc tgaactcatt gatgatattt gccaatttgc caaattgatt aatcaatatc
     2041 ggggaaccgg tattagtttg gctatcgatg actttggttc tgggtattct ggcttgaatt
     2101 tattggcaga ttttcaaccg gattctgtaa aaatcgactt atctttgatt cgcagcattg
     2161 atactcgcgg ccctcgtcaa gcaatcgtta ggggaattat tcgaacctgt caggatctcg
     2221 gtatcgatat tattgcagaa ggtattgaaa ctcccgaaga atacctttgg tgctatgagg
     2281 aagggatcga attatttcaa ggttattatt ttgccaaacc aggatttgaa cgcttgcccg
     2341 ttgctttcta ccctaagtaa agcgcaaact aaagcaggaa taatcaaaga gactctcctg
     2401 tttcaggggt ttttaaaaag ccgaattctc cttaacagtc aattaaagaa aacttttcca
     2461 ataacacttt tattatgtgc ttttcagctg aagcaagttt catcgtgagt gggacactct
     2521 ctatcattgg tatcagtgca attaggcaaa ttaaagctag aaaagacatt tttgttgcga
     2581 gcattccgct catttttgca ttgcagcaac tcaccgaggg cttactctgg gtggtgctgc
     2641 aaaatcctgg gcttgcgctt gcacaagctt ctaattggtt agctggttta tatgggattt
     2701 ttattggggt tatttggccg gtttacgcgc cgtttgcaat ttatcgagaa gaaacggata
     2761 gtcaaaccaa gcgagtcatg gcttcaatgc tattagcagg ggtaggcctt gctgcatata
     2821 cgattattgg aatgattcag gaaccgattg tagcgggcat tatcaaccat agcattcatt
     2881 atgctcatga agtggaaggc cagcaatttg ttcttgccat gtatttattt gctacttgca
     2941 caccctttat tttatcgagc agaaaattgt tgaatcttac gggaacgatc attacgctcg
     3001 gtttttttgt tgcattcttt ttctaccagc aaacctttgc ttcggtttgg tgtttctttg
     3061 cagcgattgc aagtggccta atctatttct ttattagaaa tcagactact aaataaggta
     3121 ttctcctatc agctttcatt acaagcttta ttcatttact acattcatca gccagctaaa
     3181 ccctcactga tttccaatag cgtgatttcc aatagcgatg atctaattgt gaaatggtaa
     3241 caccccggct ccgagaagca tgcatgaacc tccgattctc gagataaatt ccaacatgtc
     3301 gtgtgttatt accgattcta aaaaaaacca aatcccctgc tttcaatgaa ttgatatcta
     3361 catgccgacc cgccctcgct tggagctcag tggttcgagg aataatttta cctaattctc
     3421 gcttgtaaac cacgtgcaca aaaccggaac aatcaatccc gctacgactt aacccgcccc
     3481 attgataagg aacgccttcc cattgccggt atttggcata tagcttgccc tttacaccac
     3541 gcgaatcagt ttgaatgctc gaatcaggtg ttcgtacttg ctttgggctt cgttcttttt
     3601 gactgactga actacaacca accacgagac aacaaaagac aatgagaacg gcactgtatt
     3661 tgattggcat gttataagtt tatagagatc gataatgaag agtctatcaa aaaaaaataa
     3721 tttggaaaat aacgaataag ggccttccca gcttattctg gttgctcagg cacttgatta
     3781 taaggttttc cttcaaagaa atgggtaatg ttatcggcaa ttttatcgag caagcattgc
     3841 ctggcagcct ggctagtcca agcaatatga ggcgtaataa tcaaattagg gcgagggtaa
     3901 gttaggagta tattaccttt tgtcggtggt tcctcagaca aaacatcaag tgctgctccg
     3961 gcaatacgcc catcaattaa actttcgagt agatcagctt cattgatcaa tttgccgcgt
     4021 gccgtattga ttaaataagc ggtagatttc atcagtgcaa gctcagtacg cgtaatcaga
     4081 tgatgtgttt gcgcagtcag cgggcaatgc aaactaataa aatccgattc actaagtacc
     4141 tcctcaaagc taagtcttcc agaacgaggg ggttgccctt tatgttcagc tatccgcaat
     4201 tgcataccga atgcttgtcc gatttttcct actgctcgac ctagctcacc atatccaatt
     4261 atgccgagaa ttttcccaga taaatcttct attccgtaat ccagagaaca aaaatgggcg
     4321 caagcctgcc atttaccatg tttgatatag ctttggtatt caacgaaacg gcggaccaaa
     4381 ttgagcatca acatggccac atgctgagca accgaatcgg ttgcataatt cctcacatta
     4441 caaacgccga tattgcgctc agccgcagcg ctaagatcga cattgttata gccagttgct
     4501 gcaatacaaa tcaatttcaa gtggctagct gaatcaatca ccgctcgatc gaggaaagtt
     4561 ttactgctca cgacgacttc cgctgtttta attcgttcta cgatctgttg tggactggtt
     4621 tcatcgtagt actgccaaga cgaaattgca ttttctaatt ttgtacaatc aagatcgcct
     4681 cgggtaacag acccaaaatc taagaacact gcacgcataa catctccatt cctactttct
     4741 ccgttgcgaa acgccctcaa ccgctttgac tcgggtattg cattgtaacc catgcgttaa
     4801 gtccctacac aagatagatc aaggtggtag aatggaaagc tacctgtttt gataaatttc
     4861 ataaagagaa gatacggtca tgttttctaa acacaataca atagagcaaa ttgatccaga
     4921 tctatggtta gccattaaag gtgaaatgca acgacaagaa gatcatattg aactgattgc
     4981 ttcagaaaat tacgcaagcc ctgcagttct tgaagcacaa ggtacagtat tgaccaacaa
     5041 atatgcggaa ggttacccgg gcaagcgcta ttacggggga tgtaaacatg tcgacatcgt
     5101 tgaacaactc gccatcgatc gcctgaaatc gttgttcaat gccgaatatg ttaacgtaca
     5161 gccccattct ggctcacaag ccaatgcagc ggtttatctt tctgccctta agcctggaga
     5221 tactttactg ggtatgtcgc ttgctcatgg cgggcactta acgcatggtg catcggtcaa
     5281 tatgagtgga aaaatcttca attcgattac ttatggttta gaccctgaca ccgaagaaat
     5341 cgattacaaa caagtagaac aactggccaa ggaacatcgt cctcgcatga ttgtcgcggg
     5401 cgcctcatct tatgcccgaa ttatcgactg gaaacaattc cgtcgaattg ctgacgaagt
     5461 tggcgcctat ctttttgtag acatggccca ttacgctgga ctcattgctg caggatttta
     5521 ccctaaccca gtgggtattg cggatttcgt aacgagtacc acgcataaaa cacttcgagg
     5581 tccaaggggt ggcgttatca tggctaaacc tgaatttgag aaagcactca attcagcagt
     5641 gtttccgcaa acccaaggag gtcctttgat gcatgtcatc gcggctaaag cagtggcatt
     5701 caaagaagcg gcaagccaag ctttcaaaga ttaccaagaa caagtgttgg aaaatgcccg
     5761 tgtgatggca aaaacattgc aaaaccgtgg attacggatc gtttcaggcc gtaccgaatg
     5821 ccacatgttt ttggtcgatt tacgtgccaa gaatttaacc ggaaaggatg cagaagcagc
     5881 attagaagct gctcatatca ctgtcaataa aaatgcgatc cccaatgatc ctcaaaaacc
     5941 tttcgtaacc agtggggtac gaattggaac acctgcaatc accacacgcg gctttaagga
     6001 aatcgaaacc gagaagttag ccaatctcgt ggcagatgta ttggaagccc ctaccgatac
     6061 agccgtattg aatcgggtcg caaaagaaac tcaggattta tgtgcaaagc ttcccgtgta
     6121 tgaacgataa aagaccgtgc tttgggtaat gatcagttcg atttctgatc tattaaggta
     6181 atgcgatgcc ctttttgtaa tgctgaagat acaagcgtca ttaattcacg cgtcagtgaa
     6241 gaaggtgatc gggtaagacg tcggcgccaa tgtatcgtat gtggtaaacg ttttactacc
     6301 tatgaaacga tcgaattgcg tttaccacaa atcgtcaaac aaaacggaaa tcggactgaa
     6361 tttagtcgta ataaattgct gacgggcttt ttgcgtgctt tacataaacg gcccgtcccc
     6421 actgaagcgg ttgatgcggc aatcgatcgg attattcagc atttgctaaa ttctggcgca
     6481 cgtgaagttt catcccgtag cattggagaa ctggtaatgc aggaacttta taacatggat
     6541 aaagttgcct atatccgttt tgcttcggtc tacaagagct ttcaagatgt tgatgatttc
     6601 caggatgtca ttaaagaagt ccgggaaatc cctaaagatt aatcatcgca ttgtaaaact
     6661 tgatcgcaaa tcctttttat atcgcttctt taataaaatt gatgatagtg tacagttagg
     6721 tgtaacgatt atttgcacat aatgcctggc gaaccatcac tcttttctgc agcagattac
     6781 accttcatgg ctgaagccat tcggcttgca gaaaaaggct tatataccac gagccctaac
     6841 ccacgagttg gctgcgtcat tgtaaaaaat catcaaatta ttgggcgagg atggcatgaa
     6901 aaagcgggac aaccgcatgc tgaaatcaat gcgctaaaac aggcagcctc tgaagttaaa
     6961 ggcagtacgg tttatgttac gcttgaaccc tgttgtcact atggccgtac tcccccctgt
     7021 acagatactt tgatcaatgc aggtatcgct aaattggtca ttgctgcaca agaccctaat
     7081 ccacgagttg ccggaaaagg aataaaacaa ctcaaagcag ctggtattga agttcaatat
     7141 ggtctattgg aagcgcaagc aaaccaactc aatattggtt ttgtctcacg aatgacgcgc
     7201 caacgcccct ggattaccgt caaaatcgca tcgagcctag atggaaaaat cgccttagca
     7261 aatggtaaaa gccagtggat aactggggaa gcagccagaa cagacgtgca tcgattaaga
     7321 gcccgttcct gcgcaattct gaccggaatc aacaccgtaa aacaagatga tcctgagcta
     7381 tctgtccgct atattcaaac aactcgacaa ccatttcgtg tcatcttaga taatcgactt
     7441 gaaatcgcga cgaatgccaa ggtattacag aaacccggaa gtttaatttt cactaccttg
     7501 caagacaacc aaagaatcga ttcgttatcg gaaaaaggcg ctcgcgtcgt tgtcttatcc
     7561 agcagcacgg ggaaactcga tctcgtggaa gtgatgtcaa cattagctga gcttgaaata
     7621 aatgaattat ttgtagaagc aggcagaaca ttgaacggcg cgctcattga aagccgactc
     7681 gtcgatgaaa tcattattta ttgcgccccg atagtattag gcaaccatgc ccaaagtatg
     7741 cttaatttgc ctgagctagc atcgctttct caaaaatggc agctagaaat aaaagaaatt
     7801 ggtaaggttg gtagtgacat taaaatcaca gccaaactca tctgaatttt ctaatgcttt
     7861 ctttcaattg ctatccccat acactacctt ttcactgaat gattatgtcg ttctaagaat
     7921 gtaggtagaa atgacccgct atcgcggaat tcaggcttta ttttgctatc ccctttatgc
     7981 gctggaacaa tttcattttc gttggttcga tacaatttgc ctcgtctctg atactccaat
     8041 gcgagccgat caaatgcaaa cgttctttgt ttagcatgct gactcggaga tctttccgat
     8101 cgttttctcc gttcttgtag taaccgatat tggttgaatg caccgcaatg cacatacgga
     8161 tagcaaggat taatgaacag tggataagga tagagtaacg cctgagggta cataaacggg
     8221 ctgtagaaag tattccatcc ggcactgagt tgtagcgcca tcctaccgtc tgtttggaca
     8281 aacaatggat cgtgattttc tgttgctgcc gttgcaagcg aatttaaact cgctgaaata
     8341 acgagaaaca aagtgccaac tataaggcgt tgattaatta tcatgatgaa gctgatcctt
     8401 ctcaaaggtc ttagctattt gatacattaa ccaaattttt cgttcccgca tcacgattag
     8461 gttagccgcg ccagttgttc ggttaatttc tgtttttttg cgctgaattc tgcaactcgc
     8521 tctttctctt gtgcaactac cttctctggt gcacgtgcga taaaatctgc attatcgagc
     8581 tttgcctgtg ctttattgat ttcaccttcc aagcgagaga tttctttaga caaacgttca
     8641 cgctctgcaa caacatcgat ttcaattttg agcatcaacc ggaaatcgcg tactaccgca
     8701 acgggtgcat catcattagg taatccatcg gaaatgatgg taacttccga gagtttagct
     8761 aaggctgaaa tataggaaga gaaattgtta agtactgcag cattgccctc aatcaccaat
     8821 ggaaccctcg ctgcagggga taatttcatt tcacctcgca acgtgcgaca cgcattaatc
     8881 atttctttga gtagtacaat ttgatcgctg gcttccgtat caatcgcgct tgaatccgac
     8941 tggggataag attgcaccat gatacttcca ccctttatac ctgctagtgg gccaattttt
     9001 tgccacaatt cttcagtaat gaaaggaata atgggatgag acaacctaag cgcagtctct
     9061 aacactttaa tcaacgtata gcgcgtagtc cgttgaatcg cttcattctc agaacttaat
     9121 tgtgtttttg caagctcgac ataccaatca caatattcat cccaaacaaa ctcatagatt
     9181 tcacgcgcaa ccatatcaaa gcgataactg ttaaaacctt gttctactgc ttccaaggtt
     9241 tgctgcaagc gactgagtat ccatttatct gccaaagagt aattcaaagc atgcggcgtg
     9301 ttatcgaatc caatcgcttt cccttcacaa ttcatcaata caaagcgcgt ggcattccat
     9361 aatttattac agaaattccg ataaccctca cagcgttgca gatcgaattt gatatcacgc
     9421 ccatgtgagg caagacttgc gaacgtaaaa cgcagcgcat cggtaccaaa tgctggaata
     9481 ccttgaggaa attgtttgcg agtatttttc tcgatggatt ccgcttgttt cggattcatc
     9541 aatcctgtcg tgcgcttttt gattaaatcc gacaaagcaa tcccatcgat cagatcaagc
     9601 ggatcgagga cattcccctt cgatttactc attttctgcc cttcagcatc acggatcagc
     9661 cctgttacat acacctcttt gaagggcact tttccagtga aatgaagtga catcatcacc
     9721 atgcgcgcta cccagaaaaa aatgatgtcg aatccggtta ccaacactga agtcggtaga
     9781 aatgttttta attccgttgt ttcctttggc caacccaatg tagaaaaagg ccacaaggct
     9841 gatgaaaacc aagtatcaag cacatcttca tcttgaatta gctgatcctt cccagataat
     9901 cgctgcgcct cttccagatc atgcgcaacc gttacattac cgacttcatc ataccaagcg
     9961 ggaatacgat gtccccacca aagctggcgt gaaatacacc aatcttgaat attttctagc
    10021 cactgctgat aggtatgagt ccagttctct ggaacaaatt tgacttcacc ttgggcaact
    10081 acatcaaggc cgcgtttcgc catgccctcc atcgccacat accattgatc ggtcaacatg
    10141 ggctcgatca ccgattgagt ccggtcgcct ctcggtatc
//
LOCUS       DAOQLJ010000078        10146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2579_length_10146_cov_5.290060, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000078 DAOQLJ010000000
VERSION     DAOQLJ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10146
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2579_length_10146_cov_5.290060"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PK522_06790"
     CDS             complement(<1..679)
                     /locus_tag="PK522_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNC41476.1"
                     /translation="MTYTHDQTLPKGTHIGVYEIQDTKETTVFDITYRAWNHHLKEQV
                     EILEYFPQKLALRASGSQVVECKNPSNKEDYDLGLRAFLDQGNVLTHVEHPNIAASEN
                     ILEHNGTAYLITHRNEGKPLSRILEPSTIFEEAEIKFILISILNALKELHGYNFIHEG
                     IQPESIFLKKDGEPILTGFSSARWILARRLGEDTSTLITVYSPSEQYESTTEVKPATD
                     FYSLGATL"
     gene            1079..2071
                     /locus_tag="PK522_06795"
     CDS             1079..2071
                     /locus_tag="PK522_06795"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNC41477.1"
                     /translation="MKPPVCITVTGAAGQIGYSLIFRIAAGDMLGNDQPIILRLLDVT
                     ESQGFLNGIIMELVDCAFPLLIDVIKTDDPLVAFDKANIAILVGARPRTKGMERKDLL
                     AANSVIFSTQGKALNEVADPNVKVLVVGNPANTNAYIAMKNAPKLPINNFSAMLRLDH
                     NRALSQVAMKINQPVTEIRKMIIWGNHSSTQFPDIDHTEVRGEKIINLISDYKWVENY
                     FLPTVQNRGAAIIEARGGLSSAASAANAIINHMHDWLIGTRENDWVTMGIPSDGSYGI
                     PEEIIYGFPVTCQNGTYRIVQGLEISSRSKAKMLETYQELLEEKDSIKHLLSNR"
     gene            complement(2081..2536)
                     /locus_tag="PK522_06800"
     CDS             complement(2081..2536)
                     /locus_tag="PK522_06800"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNC41478.1"
                     /translation="MLANPIPDFELESTEGKPFRLTDYRGKKLIIYFYPKDDTPGCTT
                     ESQQFSHLYEQFKALNCEVVGISRDSIKSHENFREKLGLPFDLLSDSNETVCSRFEVM
                     KLKNMYGKQVRGIERSTFVIDEQGKLIREWRGVKVPGHVDEVLSFIKTL"
     gene            complement(2567..3907)
                     /gene="ccmI"
                     /locus_tag="PK522_06805"
     CDS             complement(2567..3907)
                     /gene="ccmI"
                     /locus_tag="PK522_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HNC41479.1"
                     /translation="MTTFWVVAGAFIVTALMFVLPTLIKSRQGKITALERQTANIAIY
                     RDQIAELDADLSNDVLSKEQYEKSKQELQKRMLLDVNASGTESGTDALPSKARGLVTA
                     VVIMLAIPLTAISLYMAIGDTRGLLPQSQLANATQFHSENMAGVPQGHAEIKSVVDNL
                     VARLQDNPEDIEGWVMLGRTYAIMGQYQQASATYAKLVEMIPDNAQLLSDYADVLAMT
                     NNGSLIGRPAELIAHALSIDPEYPKALALAGTVEFEKENYEQAANYWQRLVALIPEDS
                     QLAASVTSSIEQAKTLAVKGKEDHPVQLAQNSKVSSDLQHDATSSDEVKIAANSITST
                     KVSGKVIISPDLASKVSATDVLFVFARAKTGSKMPLAILRLSAKDLPATFSLDDDMAM
                     TPTMKMSSFPEVVIGARVSKSGQAVPTSGDLEGFSQPVKLGDKDVLITIDQIIP"
     gene            complement(3914..4375)
                     /locus_tag="PK522_06810"
     CDS             complement(3914..4375)
                     /locus_tag="PK522_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNC41480.1"
                     /translation="MSLVFTLVFFVQISFANEAIPVAEDPILEKRLIELSENLRCLVC
                     QNESLAGSRADFANDLRREIREQMKANKSDEEIIDFLVARYGDFVLYNPPFKPTTILL
                     WLGPILLFLGAILALVLYLRRRRSLVFEEPQQLSESQQLEAETLIRKIEKE"
     gene            complement(4420..4944)
                     /locus_tag="PK522_06815"
     CDS             complement(4420..4944)
                     /locus_tag="PK522_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HNC41481.1"
                     /translation="MMRFLLPLAVFIVLVVFLGIGLTLNPRQVPSPLIDKPAPVFELQ
                     QLHDPSKTLASQDNMGKVWMLNVWASWCVACRDEHPLLVQLAKLGIVPIYGLNYKDER
                     STAIQWLKQYGDPYAVSIIDADGKVGIDYGVYGVPETYVIDKKGIIRYKHIGPVTVKS
                     LEDKILPLIKELQG"
     gene            complement(4941..6989)
                     /locus_tag="PK522_06820"
     CDS             complement(4941..6989)
                     /locus_tag="PK522_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HNC41482.1"
                     /translation="MVPELGNFALILAMLLALIQGTLPLIGAARGIPAWIAIARPVVQ
                     GQFVFIAIAFGCLAYSFVNNDFSVLNVASHSNSELPLHFRFAATWGSHEGSLLLWIFL
                     LSGWSVAVSVFSKQLPDDMVARVLGVLGLVSVGFLLFLLFTSNPFDRLLPAALEGSDL
                     NPLLQDPGMVMHPPMLYMGYVGFSVAFAFAIAALLSGQLDAAWARWSRPWTTAAWVFL
                     TIGIMIGSWWAYYELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGSFKSWTV
                     LLAITAFSLSLLGTFLVRSGVLTSVHAFATDPARGIFILVYLVVIVGFSLALFAWRAP
                     KVGLGGDFQLVSRETMLLTNNVLLLVAAGSVMLGTLYPLLMDALNLGKISVGPPYFET
                     IFVPLMAPAIFLIGLGPIARWKQASLPSLVTRLRWAFVVSMVSALTMPLLMEEWKPMV
                     SFGLLLAFWIITSIVVNIKHRIQNSGQGSVIARLSRQSSSYYGMHCAHLGIAAFVIGV
                     TMVNGYETEKDVRMELGNIVSIGGYTFKFNGTTNKPGPNYKATVGDIDVLKGDTVVRK
                     LYPEKRTYNASGMVMTEAAIDTGIIRDLYIALGEPLDNSAWVVRVYHKPFVVWIWLGC
                     ILMALGGILAISDHRYRFNILRKKQSEVNQTVEKEAASIPNSTGVAISSTIVSEGKSV
                     "
     gene            complement(7033..7479)
                     /gene="ccmE"
                     /locus_tag="PK522_06825"
     CDS             complement(7033..7479)
                     /gene="ccmE"
                     /locus_tag="PK522_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNC41483.1"
                     /translation="MKPRHKKMAIIAFSLTALTGAAILVLNVFQSNLVFFFSPTQVVA
                     KEAPIGKSFRIGGLVEEGSVKRQNDSTTVNFAITDTAETIKVVYTGILPDLFKEGKGV
                     VAQGKIANDGIFYADEVLAKHDENYMPPEAAVALEQAAKAQKSTQN"
     gene            complement(7463..7627)
                     /gene="ccmD"
                     /locus_tag="PK522_06830"
     CDS             complement(7463..7627)
                     /gene="ccmD"
                     /locus_tag="PK522_06830"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HNC41484.1"
                     /translation="MGGYGLYVWGSYLATFICVIGEILMVKRSARTLRQQLSLTKESI
                     LRERKDEATS"
     gene            complement(7657..8397)
                     /gene="ccmC"
                     /locus_tag="PK522_06835"
     CDS             complement(7657..8397)
                     /gene="ccmC"
                     /locus_tag="PK522_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HNC41485.1"
                     /translation="MSINWYKYAAPSSFYFLAGKLIPFFTVLAVIFLVAGLYIGFFVA
                     PTDFQQGEAYRIIFIHVPAAWMSMFLYVVMAFWAGIGLAFNTRLSSMFATAIAPTGAL
                     FTFIALWTGAWWGKPMWGTWWVWDARLTSELILLFLYIGFMSLQAAIDDPRRADKAGA
                     VIALVGVVNIPIIYFSVQWWNTLHQGASVSVMQSPKMATAMLVGMLVMVLAAWMYSIA
                     VILLRARNIILERESHTSWVAELRGTTR"
     gene            complement(8433..9092)
                     /gene="ccmB"
                     /locus_tag="PK522_06840"
     CDS             complement(8433..9092)
                     /gene="ccmB"
                     /locus_tag="PK522_06840"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNC41486.1"
                     /translation="MLMWIIRRDLMLAMRRQADVLITLFFFIIVVSLFPLGVGPEMNI
                     LRAIAPGVLWVAALLASMLSLGRLFSIDYMDGTLEQLLIAPSPLFLLVLGKMISHWLV
                     SGLPLVLMAPILGIQYDLSLEALGVLTLSLLIGTPILSLIGAIGAALTLGVRGGGVLV
                     SLLVLPLYIPILIFGAGAVEASAAGLGYDAHFSLLGAFLLVSLVFAPWAAAASLRISM
                     E"
     gene            complement(9099..9713)
                     /gene="ccmA"
                     /locus_tag="PK522_06845"
     CDS             complement(9099..9713)
                     /gene="ccmA"
                     /locus_tag="PK522_06845"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HNC41487.1"
                     /translation="MLAATKLECTRGGHVLFTDLNFSVKSGELLQIHGPNGSGKTSLL
                     RMLCGLTQPSVGKITWNDSNIRSLGEQYYSCLTYLGHMIGIKDDLTAVENLSISCALA
                     GLEVDNRMALEALQQIGLKGRENLLARVLSQGQRRRVSLARLLLVKTKLWILDEPLTA
                     LDVAAIKLIKEKLEWFLAQGGMVILTTHQDIDISATAIHHLHLN"
     gene            complement(9767..>10146)
                     /locus_tag="PK522_06850"
     CDS             complement(9767..>10146)
                     /locus_tag="PK522_06850"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:NF027523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNC41488.1"
                     /translation="IRTLAEDIGKAMGWGGAYLTDLCRSQVGLFCLDQAYGLDQFQDD
                     VNARHDSLLYPVDSLLQGYHSVVLSEEQVNLLFNGQVVSKENEQELPMGNKVKLYDLR
                     GNFLGIGETIQANQIRPKRLVSH"
BASE COUNT         3197 a         2346 c         1724 g         2879 t
ORIGIN      
        1 atagcgtcgc gccgagtgaa taaaaatcgg ttgcgggctt aacttccgtt gtagattcat
       61 attgttctga gggcgagtaa actgtaatca aggtagaagt atcttcaccc aatcgccttg
      121 cgagtatcca acgcgcagat gaaaaacccg tcaagatagg ctcgccatct tttttcaaga
      181 aaatcgattc gggctggatc ccctcgtgaa taaaattata tccatgcagt tcttttaagg
      241 catttaagat tgatatcagt ataaatttga tctctgcctc ttcaaaaatt gttgatggct
      301 ctagtatgcg agaaagtggt ttgccttcat tacgatgtgt tatcaaataa gctgtcccgt
      361 tatgctccaa gatattttca gaggcggcta tattcgggtg ttccacgtga gtaagaacat
      421 ttccttgatc tagaaatgcc ctcaaaccca agtcataatc ttccttattc gatggatttt
      481 tgcattcaac aacttggcta ccgctagcac gtaatgcaag cttctgagga aaatattcaa
      541 gtatctcaac ttgttctttt agatgatgat tccaagcacg ataagtaata tcaaataccg
      601 tagtttcttt cgtatcttga atttcataga caccaatatg tgtacctttt ggcagagtct
      661 ggtcgtgagt atatgtcatg atattgttgt cctttttcct atactttata ttaacgtgtt
      721 gccttaaata ttaaatcatt tagcgagttg aattcatcta ggggaaatgg ctgattacgt
      781 atctcagtct tagattttca atgatacata attccattga ccactatctc tttgatgaaa
      841 ataacctttc ccacaaactt taccatgatg cgctctatcg taattaaatc ttcatccata
      901 tctctcatta tccaacttaa aatcgatcaa acaaaatgat tgaaccagca gtattgcgat
      961 aaccaattat cttacaaata gtacccagtt agctctgagc agaaatcgtt tagaatcctg
     1021 actaaaccgc gatacttcga tatactatat aaaatacatt taattaaaaa ttgatagtat
     1081 gaaacctcct gtttgcatca cagtcacagg cgcagctggg caaattggct acagcttaat
     1141 atttcggata gcggctgggg acatgcttgg taatgatcaa cccatcattc ttcgtttact
     1201 ggatgttact gaatcgcaag gttttcttaa tggtatcatc atggagcttg ttgattgtgc
     1261 ctttccgctc ttaatagatg tcattaaaac tgatgatcca ctcgtcgctt tcgataaggc
     1321 taatattgcg atcctagtcg gcgctcgacc acgaacaaaa ggaatggagc gtaaggacct
     1381 gctagctgct aatagcgtta tattcagtac ccaaggtaaa gcactcaatg aagttgctga
     1441 tccaaacgta aaagtgctcg ttgttggcaa tccagccaac acgaatgcat atattgcgat
     1501 gaagaatgca cctaaactac caataaataa cttttcagca atgctccgat tggatcacaa
     1561 tcgagcattg tcccaggtag ctatgaaaat taatcaaccc gttacagaaa ttagaaaaat
     1621 gattatctgg ggcaatcatt cttcaactca atttcctgat attgatcaca cagaggttag
     1681 aggcgagaaa ataatcaacc ttattagtga ttacaagtgg gttgaaaatt acttccttcc
     1741 aactgtgcaa aaccggggtg cagctattat cgaagcgaga ggtgggttat cgagcgctgc
     1801 aagcgctgcc aatgcaatta ttaatcatat gcatgattgg ttgattggaa ctcgagaaaa
     1861 tgactgggtg acaatgggga taccctctga tggtagttat ggcatcccag aggaaattat
     1921 ttatggattc cctgttacat gtcagaatgg cacttacagg attgttcaag gacttgaaat
     1981 cagtagtcga tctaaggcta aaatgcttga aacctaccaa gaactcctcg aagaaaaaga
     2041 ttcaatcaag catttacttt caaaccgatg aaacttcaat tcaaagtgtt ttaataaatg
     2101 atagaacctc atctacatga ccaggcactt tcacaccacg ccactctctt attagcttgc
     2161 cctgttcatc gataacaaaa gtactccgtt caataccacg aacttgtttg ccatacatat
     2221 ttttcaattt catgacttca aagcgactac aaaccgtttc gtttgaatca cttaataaat
     2281 caaatggcaa tcctagtttt tctctaaaat tttcgtgtga tttaatgcta tctcttgaaa
     2341 ttccaacaac ttcacaatta agtgctttaa attgttcata taaatggcta aattgctgac
     2401 tttcggtggt acaacccggc gtgtcatctt ttggataaaa atagattatc aacttcttac
     2461 cacgatagtc agttaaccta aaaggtttac cttcagtcga ttcaagttca aagtcaggta
     2521 taggattagc taacatctga gtatcaatta ataaagaaat ttatgtttag ggtatgattt
     2581 gatcaattgt aatcaaaaca tctttatcgc ccaatttaac tggttggcta aatccttcta
     2641 agtctccact tgtcggaact gcctgacctg atttacttac cctagcccca ataactactt
     2701 caggaaaact agacattttc atggtaggag tcatagccat atcatcgtca agtgaaaaag
     2761 ttgctggtaa atccttagca cttaatctga ggattgctaa aggcatttta gatcctgttt
     2821 ttgcacgagc aaaaacaaac aaaacatccg tagcggatac tttacttgcc agatcaggac
     2881 taatgatcac ttttccagat acctttgttg atgtaatcga atttgctgct atcttaactt
     2941 catctgaaga cgtagcatca tgctgtaaat cacttgagac ctttgaattc tgagctaatt
     3001 gaactgggtg atcttctttt ccttttacag caagtgtctt ggcttgctcg atgctactag
     3061 tcactgatgc tgctaattga gaatcttcag gaataagcgc caccagcctc tgccagtaat
     3121 ttgctgcttg ttcatagttt tctttctcaa attcaactgt tccggccaga gcaagtgctt
     3181 tcgggtattc tggatcaatg ctcagtgcat gagcaatcaa ctcagctggt ctaccaatca
     3241 agcttccatt attcgtcatt gccaacacat ctgcatagtc acttaataat tgcgcattgt
     3301 cgggaatcat ctcgactaac ttagcataag tagcactcgc ttgttgatat tgacccatta
     3361 tggcgtaggt tctgcctaac ataacccatc cttcgatatc ttccggatta tcttgaagac
     3421 gcgctactag attatcaaca actgatttaa tctcagcatg cccttgtggc actccggcca
     3481 tattttcaga atgaaactgt gttgcatttg caagctgcga ctgtggcaac aatccccgcg
     3541 tatcaccgat cgccatatac aacgatatag ctgttagggg aatagcaagc ataatcacaa
     3601 ccgcagttac taaaccacgc gctttactgg gtaatgcatc ggtgccggac tcagtgcccg
     3661 atgcattgac atctagcaac attctttttt gtaattcttg tttactcttt tcgtactgtt
     3721 ccttagataa aacatcgtta ctcaaatctg catctaattc agctatctga tcacgataaa
     3781 tcgcaatatt agctgtttga cgctctagtg ctgttatttt tccctgccga ctcttgatca
     3841 aggttggcaa aacgaacatc agcgcagtaa caataaaagc acctgcaaca acccaaaatg
     3901 tagtcatcct tttttattcc ttctcaattt ttctaatcaa ggtttctgct tctagctgct
     3961 gactctcaga tagttgctgc ggttcttcaa atactagcga tcgacgacgt cttaaatata
     4021 aaactaaagc aaggattgcc cctaagaata ataagatggg gccaagccat agcaatattg
     4081 tcgttggctt aaacggtgga ttgtaaagaa caaaatcacc gtatcgcgca actagaaaat
     4141 cgattatttc ttcatcgctt ttattagctt tcatttgttc tcgaatttct cgcctcagat
     4201 cattcgcaaa atcagcccgt gagccagcca gtgattcatt ttggcaaact aaacagcgta
     4261 agttctccga aagctcaatc aaacgttttt ctaaaatggg gtcttcagct actggaattg
     4321 cttcattagc aaaacttatc tgtacaaaaa atactaatgt aaatacaagt gacatctgcc
     4381 agcttgacag aaaatgactg tttaaataat tcattaatct caaccttgca attcttttat
     4441 cagaggtagg attttatcct ccaatgactt caccgtgacg ggtccgatat gtttataacg
     4501 aatgatccct ttcttatcaa taacataagt ctcaggaact ccatataccc cataatcaat
     4561 ccctaccttt ccatcagcgt caataatact taccgcatag ggatcaccat attgcttcaa
     4621 ccattgaatt gcagtacttc gctcatcttt ataattcaat ccataaattg ggactatgcc
     4681 aagtttagca agctgaacta acaatggatg ctcatctcga caagctacac accatgaggc
     4741 ccaaacattt agcatccaga ccttacccat attgtcttgc gatgctaatg tcttcgaagg
     4801 atcatgtaat tgctgtaatt caaagacagg tgcaggttta tcaatgagcg gagaaggaac
     4861 ttgacgcgga ttcagtgtta atcctatccc taaaaaaacc actaatacaa taaatactgc
     4921 taatggcaat agaaagcgca tcatacgctt ttaccttctg aaacgattgt cgagctaatt
     4981 gcaacgccag tggaatttgg aatcgatgcc gcttcctttt caacagtctg attaacctca
     5041 gattgttttt ttctaagaat attaaatcga taacggtgat ctgaaatagc cagaattcct
     5101 cccaatgcca tcaaaataca accgagccat atccaaacaa caaagggctt atgatacaca
     5161 cggacaaccc aagcactgtt atccaacggc tctcccaatg caatatacaa atctcttata
     5221 ataccagtat ctatcgcagc ttctgtcata accataccgg atgcattgta agttcttttc
     5281 tcaggatata atttgcgcac cacggtatca cctttcagca catcgatatc acccactgtc
     5341 gctttgtagt ttggtcctgg cttatttgta gttccattaa acttaaaggt atagccgcca
     5401 attgatacaa tatttccaag ctccatccga acatctttct cggtttcata accattaacc
     5461 atcgttacac caattacaaa agctgctata cctaagtgcg cgcaatgcat tccatagtaa
     5521 ctagaggatt ggcgactcaa tctcgctatg acgctcccct gcccactatt ttggatacga
     5581 tgtttaatat tgacaacaat acttgtgata atccagaacg ccaaaagtag accaaaacta
     5641 accatgggct tccattcttc catcaataaa ggcatggtca gagcgcttac catactaacc
     5701 acgaatgccc agcgtaaacg agtgactaat gacggtaaac ttgcttgctt ccaccgagca
     5761 attggtccaa gaccaatcaa gaaaatagct ggtgccatca aaggaacaaa tattgtttca
     5821 aaatagggag ggccaacaga tattttgcct aaattcaacg catccattaa taaaggatag
     5881 agtgtgccaa gcatgacact ccctgcagcc acgagcaata agacattatt agtcaataac
     5941 atcgtttcgc gtgacaccaa ttgaaaatca ccgcctaagc ctactttagg cgcacgccac
     6001 gcgaataacg ctaatgaaaa acccacaatc actactagat aaactaaaat gaaaatacca
     6061 cgggctggat cagttgcgaa tgcatgaact gaagtcaaaa cacctgaacg caccaagaat
     6121 gtacccaata agcttaatga gaatgctgta atcgccagta aaaccgtcca gcttttgaaa
     6181 ctaccacgct tctcagttac agccaaagaa tgaatcagtg cggttccaac taaccatggc
     6241 ataaaagatg cattctcgac aggatcccaa aaccaccatc caccccaacc taattcataa
     6301 taggcccacc aactaccgat catgatacca atcgttaaga atacccatgc tgcagtggtc
     6361 caaggacgtg accaccttgc ccatgctgca tcgagctgtc cactcaataa cgcagcaatt
     6421 gcaaaggcaa atgcaaccga aaacccaaca tatcccatat aaagcattgg aggatgcatg
     6481 accattcctg gatcttgtaa taatggattc aggtcacttc cttcaagcgc agcgggtaat
     6541 aaacgatcaa atgggttaga ggtaaataat aaaaataaca aaaatccaac gctaaccaat
     6601 cccaaaacac ctaagactct cgcaaccata tcatcaggca attgcttact aaatacgctg
     6661 acagcaacag accaacctga taacaagaat atccatagta ataacgaacc ctcatgtgat
     6721 ccccaagttg cagcaaatct gaaatgaagc ggtaattcag aattggaatg agatgcgaca
     6781 ttgagtactg aaaaatcatt attaacaaaa gaataagcta agcacccaaa tgcgattgca
     6841 ataaatacaa actgcccttg tactactggc cgcgctattg ctatccaagc aggaatacca
     6901 cgagccgccc cgattaatgg gagcgtgccc tgaatcaatg ctaataacat tgcaagaatc
     6961 aaagcaaaat taccgagttc tgggaccata attattaagt attaaagatt acaatgaaaa
     7021 aagattattc tattaatttt gagttgattt ctgggcttta gctgcttgtt caagtgcaac
     7081 agctgcttct ggaggcatat aattttcatc atgtttcgca agaacctcgt cagcataaaa
     7141 aatgccatca tttgcaattt tcccctgcgc cacaacccct ttcccttcct taaataaatc
     7201 aggaagtatc ccggtataga ctacttttat ggtttctgcc gtatcggtaa tcgcaaaatt
     7261 aacggttgtg gaatcattct ggcgtttaac gctaccttct tccaccaacc caccaattcg
     7321 gaaacttttc ccaattggcg cttcctttgc tacaacctga gttggactaa agaaaaacac
     7381 aagattactt tggaacacat tcaacaccaa aattgctgca cccgttaatg cggtaaggct
     7441 aaaggctatt attgccatct ttttatgacg tggcttcatc ttttctctct cttaaaattg
     7501 actcttttgt gagactcagt tgctgcctta aagttctagc gcttctttta accatcagaa
     7561 tctcgcctat aacacatata aatgtagcta gataagaccc ccaaacatag aggccatatc
     7621 ctcccatatt aaaaaaatcg gaccagctat cccagatcat cgcgttgttc ctcttaattc
     7681 agctacccag cttgtatgac tctcacgttc caatataatg ttccgtgctc taagcaagat
     7741 taccgctata gaatacatcc aagcagccag caccatcact aacattccaa ccaacatcgc
     7801 agttgccatt ttaggtgact gcatcacgct aaccgatgct ccttgatgca aagtattcca
     7861 ccactgaacc gaaaaataga taattggaat attgacaacc ccgaccaacg ctattacagc
     7921 accagcttta tcagctcttc tcggatcatc gatagctgct tgtagtgaca taaatccaat
     7981 atataaaaac aataatatca attcagaagt caatctagcg tcccaaaccc accaagttcc
     8041 ccacatcggc ttcccccacc acgcacctgt ccataaagca ataaatgtaa ataaagcccc
     8101 agtcggtgct atggctgtag caaacataga tgaaagtcga gtattaaacg ccaatcctat
     8161 ccctgcccag aaagccataa caacgtataa aaacatcgac atccaggccg ctggaacatg
     8221 aataaagata atgcgatatg cctctccctg ctggaaatca gttggcgcca caaaaaaacc
     8281 aatgtatagc cctgcaacaa gaaagattac tgctaaaaca gtaaaaaaag gaattaattt
     8341 cccggcaagg aaataaaaac tcgagggtgc tgcatactta taccaattta ttgacatact
     8401 aactaatttt ataaatttac taaaataata ttctactcca tcgaaatcct tagcgatgca
     8461 gcggctgccc aaggagcaaa aacgagtgaa accaatagaa aagcgccaag caatgaaaaa
     8521 tgtgcatcat accctaaacc tgcagcgctt gcttcaactg caccggcccc aaaaattaat
     8581 atggggatat aaagtggtaa tactaataac gaaactaata cccctcctcc cctaactccc
     8641 aatgtcagcg ctgcaccgat tgcgccaatc aaactcagta ttggggtccc tatcaataat
     8701 gatagcgtta gaactcccaa ggcttccagg gacaaatcgt attgaatacc aagaataggt
     8761 gccattaata ctaaaggcaa accggaaact agccaatgtg aaatcatctt tcccaatacc
     8821 aaaagaaaca aaggcgatgg tgcaatcaat aattgctcta atgtaccatc catataatcg
     8881 atggaaaaca atcgacccag tgacaacatc gaagctagta gtgcagctac ccataataca
     8941 cctggcgcta tggccctcag aatattcatt tctggaccaa cacccagtgg aaataggcta
     9001 actacaataa taaaaaaaaa caatgtgatc aacacatcag cctggcgacg catcgctagc
     9061 atcaagtctc ttctgattat ccacatcaac atttcatttc aattcaagtg taggtgatga
     9121 atcgctgtcg ctgaaatatc gatgtcttga tgtgtcgtca gtattaccat cccaccttga
     9181 gcaagaaacc attctaactt ttctttgatt aattttatag ctgccacatc cagcgcagta
     9241 agtggctcat ctaaaatcca caattttgtt ttaactagca ataatctcgc cagagatact
     9301 cttcttcttt gtccttgtga caacactcgg gccagcaagt tttctcgccc ctttaaacca
     9361 atctgttgta gcgcctcaag cgccattctg ttatcaactt ctaaaccagc cagtgcgcaa
     9421 gaaattgata aattctcaac tgccgttaga tcatccttta ttccgatcat atggcctaaa
     9481 taagtaagac agctatagta ctgttcgcca agtgatcgga tatttgaatc attccaagta
     9541 atcttcccga cgcttggctg tgtcaatcca cataacattc tcaacaaact tgtcttaccg
     9601 ctaccattag ggccgtgaat ttgtagcaat tcacctgatt tcactgaaaa attaagatcg
     9661 gtgaataaaa cgtgtcctcc ccgtgtacac tctaatttgg tagctgctag catttattat
     9721 ttataagact aagtttcaat tcaaaatctc aacgcatcgt taatgttcaa tgtgacacta
     9781 atcttttggg ccttatttga ttggcttgaa tcgtttctcc aattcccaga aaattccctc
     9841 gtaagtcata taacttaact ttattgccca tcggtaactc ttgctcattt tctttactaa
     9901 cgacttggcc attaaacaac aaattaacct gttcttcact taacactact gaatgatatc
     9961 cttgcaaaag agaatcaact ggatagagaa gtgaatcatg tcgtgcattt acatcatctt
    10021 gaaattgatc aagcccataa gcttgatcta agcaaaacaa ccctacttgg cttctacaaa
    10081 gatcagttaa ataagcgccc ccccaaccca ttgccttacc tatatcctca gctaaggtac
    10141 gaatat
//
LOCUS       DAOQLJ010000079        10032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2610_length_10032_cov_5.420367, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000079 DAOQLJ010000000
VERSION     DAOQLJ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10032
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2610_length_10032_cov_5.420367"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PK522_06855"
     CDS             complement(<1..538)
                     /locus_tag="PK522_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC41489.1"
                     /translation="MKRTEWLQETRKMRFEEAYEGYRSKSLTQREAALLLGVCDRTFR
                     RYMDRYEEGGLGALLDKRLTQASHRCAPIDEVMQLTQQYRKDYSGWNAMHFHAWYRRD
                     GGTRSYTWVKNQLQEAGLIKRSTKRGAHRMRRERSPLPGMMIHQDGSTHEWVVNQKWD
                     LIESLRVFRRPFSLSQATL"
     gene            703..1527
                     /locus_tag="PK522_06860"
     CDS             703..1527
                     /locus_tag="PK522_06860"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNC41490.1"
                     /translation="MADIFLSYASSNRTNAETLAIVIQKLGWTVWWDRQIPIGLTYDE
                     VIEKNLKAAKCVIVLWTKASAASDWVKNEASEGAKRKILIPILLENIEIPLEFRRNQT
                     AKLIDWRSATQYHPEFPKLYTRLTELLGHPRYSPEGPPFKEFNHQPSIVIEAPPPPPW
                     RKFFIVAAVILSLVVIVPPLVNIVNPPPTTSSSAQIDELPPLNMSAEQSYQKGKQLFD
                     SKKYKEAFIWLEAAAHQDHIGAINKVIEMYSDGKGVDENYGKVAEWVEKKSFLHKE"
     gene            complement(1541..2152)
                     /locus_tag="PK522_06865"
     CDS             complement(1541..2152)
                     /locus_tag="PK522_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41491.1"
                     /translation="MKRLIIVPLAATAILSGCAHTTNLSKEGQAGIGAGLGGALGAAI
                     GYAVCGAECAAIGAGAGIVAGGLTAYALASDPYTQSAAQQAESWKKETGSEAEVVKVS
                     DVTENGQKRQRLDVQKMQLSDAQVVTKDRLSPRIKEQLINTKSSALKTGGYVHVVCPA
                     NAPSSVMSDIDKIGIGYSKDTTTASGYTIYLSRDRAELKSLNI"
     gene            complement(2196..3575)
                     /locus_tag="PK522_06870"
     CDS             complement(2196..3575)
                     /locus_tag="PK522_06870"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNC41492.1"
                     /translation="MDQIGHYKIIKKIGEGGMGEVYKGVDTMLEREVAIKLMRSELSN
                     RPDIVERFRSEAIALGRLNHSNIAAVYNFGQVESQYYMALEFVSGKTLDSIIKKLGKV
                     PWREALNYAIAALDGLEHAHQLKIIHRDIKPANIMINEQGNVKIMDFGIARILGKERM
                     TQIGRFIGTLEYMPPEQIQGKEADARSDVYAIGAVLYEMLTGHILFERDTEFELLNSQ
                     ISEKPKPLREFNHEVSPELEKIILRALEKEPGKRYASAREFSEVIRRLLLAEPSASLT
                     HQNSLIAFCKDYPAVAIAASLLLPVGSYIAWQLNFADPSQVSQSVVFPVEKLVVEPTQ
                     RAIVENQSIEAERITAITSEKIPEMRNSLQSFPPNLPSSGNVDIGDSTVEDTFDIESE
                     LMHVLPMPERKPEVPEYFVQTNKQSAKQISTSAQQNIVTKTKPSSSASDKPVDDPRID
                     EWAKDFFEN"
     gene            complement(3608..4396)
                     /locus_tag="PK522_06875"
     CDS             complement(3608..4396)
                     /locus_tag="PK522_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNC41493.1"
                     /translation="MASYFHLEIGSLSDTGRCRSVNQDCLGIDGFDSSHNFLAIVADG
                     MGGHQAGDIASRIAVDEIKHHYFVLLKKQSPDQALRQAFQEANTAILTRAQQFPEYQR
                     MGTTLVVLSLNKSAAYFASTGDSRLYLIRENAIQQLTRDHTLVAEMLRKGLLSVEQAK
                     NHPDKHIITHAVGTQEKVFVDTSDLPLLLKYQDCFLLCSDGLYDLVSDAEMLDYATQY
                     PVQQACQKLVDLANTRGGYDNISVIIIKILEKPGLTNEIPITQS"
     gene            complement(4486..6339)
                     /locus_tag="PK522_06880"
     CDS             complement(4486..6339)
                     /locus_tag="PK522_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNC41494.1"
                     /translation="MQKVIVKHMTGSKANQTEIFELPIDAITFGRDTNCKVAYDPLKD
                     DLVSRTHLKITLVEDDQFLLTDLGSSNGTFVNEQKIAKPVILQAGDIVQLGKNGPKFE
                     FDLDPRPPVAAKATRLQEVPIPKSTRESQVSSTVESQLSRTDIQNISTNQKQGVGRET
                     VERLIDRVESDARVKIAETEGRARRKMINVSAGIFTVIVLVAGYFFYQNQQSQKQLQA
                     TEEQLKSNIAAAKEEQSRQMEEMKSNTPMSATEISQKYESAVVYIETSWKLKDVESGQ
                     QLYHGRVCQKSKSEKRCLPAYVSFQREGKEYVEPLLFTENVRDELFSSFGIETERGVA
                     IGKSNTGSGFVVDMNGFILTNTHVAAAWEVAYDISQLENGFVIRCNDEGKNCKVKGNI
                     ADTKYLRLLEKWIPANTQYLGGRPQTGKYVEGYHDYLEVTFPGLGQRSNARLTKISGN
                     ADVALIKVDAMKKLVHVELNSGRSISTGEPVTVMGYPIISPDTSVTRQNRKVPEPTIT
                     TGIIGKVIENKAGVTLDTVGKNVEMILFKGLNREDFLQKHMIHKEDGSLLVNELLYQL
                     TINAAGAGTSGGPVFDSKGQVIGIFTDLIRDEQGTQVSLALPIKYGLEIMK"
     gene            complement(6828..7055)
                     /locus_tag="PK522_06885"
     CDS             complement(6828..7055)
                     /locus_tag="PK522_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41495.1"
                     /translation="MKEEIHQRLEVLRGEYEKGQIRLRELQQQELHLQETLLRISGAI
                     QALTELLEQSSIDSQKIDLKKVNDNVSTNTS"
     gene            complement(7091..>10032)
                     /locus_tag="PK522_06890"
     CDS             complement(7091..>10032)
                     /locus_tag="PK522_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41496.1"
                     /translation="NGGCFTGLGTTRLELLGYPIKITANQLSSPEIIYHNAQPTGEWR
                     KTKDPQAFRLLPGLYNLQVAANYANVSYRVTPQGKIDYEDTLDIKNGGCFSGKESTQL
                     ELLGYSIEIIAEKLSTEQIAYPYIIPSLGSKSLQTFRLLPGQYNLQVAAGYATVIYLV
                     TPQGKIDYDEDLDANNGGCFAGLGTTRLELLGYSVEIVADEKLSTPQITYPYIISGWV
                     SGKTKQIFRLLPGLYNLQTPASFVSVVYTVTPQGKIGYEEPLDVSNGGCFAGKETTQL
                     KLIGFSIAVDSSPLFQPQFLISEATPSDEVAIQKKVTFHLLPGAYTIQSISGSGYIFS
                     VTPKGTIDYDETFDVNNPEAPGFFSGSETTTLTLEGYPITLDATPLADAISQFGLGNG
                     TRWLTTRESHTVRLFPARYGLDLASGVINGAEFEVIFGGTIDYDSSLDIQRASGDLRP
                     PGYLSGNNTNKLRLEGYAITLDASPLAQIAGSFRLGSGDATSRLPTNKASKVRLLPTH
                     YSLYFDTGAIAEHLFEVLYGGVIRYATQFDWSNGGFLEGNGEPRLKFLGYTLLIDASE
                     LSGTKVVLLPFQYSIGSVSKGIEAIQVMPQANLTLELFTISPQSVVFDMELNGTVTLR
                     KSFPFVILEISSGYPLLKLKKHSLQLEQNNKRAMKINLPISIPLPNLDDKSFSDLVDE
                     GKRLIPSFAPSWTDHNTSDPGITFIELFAFITEMLLYRANRITDENKRAFVQLLRGPK
                     YILGNSIDEEIRLAILELRSEQRAVTSEDFKRIAMEFRIGGDAIIARALCLPCYNLES
                     SVIKPNASAHVSLIILTKNTKPDQPPEAESSLCAAIKEYIKPRCLLTTRLHVVSPFFL
                     RISAHFVVYIFADQDEVVMENLVKKELENYFHPLTGGLDKQGWPLGRSVYISDLFVLL
                     DGIDGVDYVTPDKDSPMLESPNNDTRRIVENNTKKTIGYRLEPYELVYFSPGDSKITA
                     VRQSILQ"
BASE COUNT         2803 a         2270 c         1897 g         3062 t
ORIGIN      
        1 ctaatgtggc ttgactcaaa ctaaaaggtc tccggaaaac ccggagcgat tcaatcaaat
       61 cccatttctg attaacgacc cattcatgtg tgctgccatc ctgatgaatc atcattccag
      121 gtaatggtga acgttcacgg cgcatccggt gtgcgccacg ctttgtgcta cgcttgatca
      181 accctgcttc ttgcagctga ttcttaaccc atgtatagct gcgtgtgccg ccatcccgac
      241 gataccatgc atgaaaatgc atggcattcc aaccggaata gtctttgcga tactgttgcg
      301 tcaattgcat aacttcatca atcggcgcgc agcgatggga tgcttgagtc aatcgcttat
      361 ccaataatgc acccaaacca ccttcctcat atcgatccat ataacgccga aatgtccgat
      421 cgcatacccc tagcaataat gcagcctccc tttgcgtcaa actcttactt cgatagcctt
      481 catacgcttc ctcaaatctc atcttccggg tctcctgtag ccactctgtc cgcttcattt
      541 gaaatcctcc tcggattcca tataaatccg gacagatctt gtgctacaac cccggacagt
      601 ttatttgttc tctacaaact ccaaaataca cttctagtcc taacactctt tcgatagaat
      661 atacctatct cgaatattta tggcagtgga ggtaggggat taatggctga tatctttcta
      721 agttatgcta gttcgaatcg tactaacgct gagactttag ctattgtgat acaaaaactc
      781 ggctggacag tatggtggga tcggcaaata cctattggat taacttatga tgaagtcatc
      841 gaaaaaaatc ttaaagctgc aaaatgtgta attgtgctgt ggaccaaagc atcagctgcc
      901 tcagattggg tgaaaaatga agcctcagaa ggtgctaaac gtaagattct aattcctatt
      961 ctgctagaaa atattgaaat tccgctagaa tttaggcgca atcaaactgc taaattaatc
     1021 gactggcgat cagcaactca gtatcatcca gagttcccaa aactatatac acggcttact
     1081 gaacttctgg gtcatccacg gtattctcct gaaggaccac catttaaaga atttaatcat
     1141 cagccaagca ttgttattga agcccccccg cctccgccgt ggagaaagtt ttttattgtg
     1201 gcagcagtca ttctttccct ggtcgttata gtgccgccat tggtaaatat tgttaaccct
     1261 cctcctacca cttcttcgag cgcacaaatt gatgaattac ctccattaaa tatgtcggct
     1321 gaacaaagct accaaaaagg taaacagtta tttgattcta agaaatataa agaagctttc
     1381 atctggcttg aagctgcggc acatcaagat catattggag cgataaataa ggttatcgag
     1441 atgtacagtg atggtaaagg agttgatgaa aattacggta aagtagcgga atgggtagag
     1501 aagaaaagtt tcctccataa agaatagatt aatgaactaa ttatatattt agtgacttta
     1561 actcagccct gtctctagaa agataaatag tataaccact ggcagtggtt gtgtctttac
     1621 tatagccaat cccaatttta tcaatatcgc tcattacgga agaaggtgcg tttgcgggac
     1681 agactacatg aacatatcct ccagttttaa gagctgaact ctttgtgttg attagttgtt
     1741 ccttaattct tggtgacaat cgatctttag tgactacctg agcatctgat agttgcattt
     1801 tctgcacatc cagtcgttgc cgtttttgac cattctcggt tacatcagat actttgacaa
     1861 cttctgcttc gctaccggtt tcctttttcc aggactctgc ctgctgcgct gctgattgtg
     1921 tatagggatc actagctagc gcatatgcag tcaatccacc agcaacaata cctgctcccg
     1981 caccaatcgc tgcacactcc gctccacata ctgcataacc aattgccgca ccaagggcac
     2041 cacctaaacc cgcaccaata cctgcctgac cttccttact caggtttgtc gtatgagcac
     2101 aaccactgag gatagccgtt gcagcaagtg gtactattat caatcgtttc attattaatt
     2161 tcccttcatt agatttatta aacataaagt aaatgttaat tctcaaaaaa atctttagcc
     2221 cattcatcaa ttctaggatc atcgactggt ttatcactag cagaggaaga aggtttcgtt
     2281 ttagttacga tattctgctg cgctgatgtt gatatttgct tagcagattg cttatttgtt
     2341 tgaacaaaat actcaggcac ctccggtttc ctttcaggca ttggcaagac atgcatcaat
     2401 tctgattcta tatcgaatgt atcttccacg gttgagtctc ctatatctac attcccggac
     2461 gagggtaaat ttggtgggaa tgattgtaaa gaattcctca tctcaggtat tttctcagat
     2521 gtaatcgctg taattctttc agcttcgata gattgattct caacaattgc tctttgggta
     2581 ggctcaacca ctaacttctc tacaggaaat accaccgatt gggaaacctg agaaggatca
     2641 gcaaaattta attgccatgc tatgtaacta ccgacaggta gtaaaagtga ggcagcgatt
     2701 gcgacagctg gataatcttt acaaaaggca atgagagagt tttgatgagt taaggatgct
     2761 gatggttcag caagtaataa acgacgtatt acttcgctga attcccgtgc actggcgtaa
     2821 cgtttcccgg gttctttctc caaagcacgc aatatgattt tttctaattc aggtgagacc
     2881 tcatggttga attcgcgtaa tggttttggc ttttctgaga tttgtgaatt taataattca
     2941 aattccgtat cacgttcgaa aagaatatgc cctgttaaca tttcgtataa gactgcacct
     3001 atagcataaa catcagagcg tgcatcagct tcttttccct gaatctgctc aggcggcata
     3061 tattctaagg ttccgataaa acgccctatc tgagtcattc gctcttttcc cagaattctg
     3121 gcaattccga aatccatgat tttaacgttg ccttgctcat ttatcatgat atttgcgggc
     3181 ttaatatcac ggtgaatgat ttttagctga tgtgcgtgtt ccagtccatc caatgcagca
     3241 atcgcgtaat ttagcgcttc tcgccatgga accttaccga gctttttgat aatgctatct
     3301 agcgttttac cactgacaaa ttccagcgcc atgtaatact gactttccac ctgcccaaaa
     3361 ttataaactg ctgcaatatt ggaatgattg agacgaccta atgcaatcgc ttctgaccgg
     3421 aaacgctcga caatgtctgg gcgattactt agttctgaac gcatgagttt gattgcaacc
     3481 tcgcgctcta gcatagtatc cacacccttg tagacttctc ccatacctcc ttcgccaatt
     3541 tttttgataa tcttgtaatg cccaatttga tccataatgc aatatctttt aaaaattttg
     3601 aattttgtta gctttgggta attggtatct cattagttag tcctggtttt tccagaattt
     3661 ttatgataat tactgaaata ttatcatagc cacctcgggt attcgctaaa tctaccagct
     3721 tctggcaagc ctgttgcaca ggatattgcg ttgcataatc aagcatctct gcatcgctta
     3781 ccaaatcata caagccatct gaacacaaca ggaaacaatc ctgatacttt aataataatg
     3841 gtaagtcgga agtatcgaca aaaacctttt cctgagtacc aacggcatga gtaatgatgt
     3901 gtttatccgg atgatttttt gcttgctcta cgcttaataa tcctttcctt agcatttcag
     3961 ctaccaaagt atgatcccga gtaagctgct gaatagcatt ttctcgaatt aggtaaagcc
     4021 gactatcccc agtagaagca aaataggctg cagacttatt cagtgatagc acaacgagcg
     4081 ttgtgcccat tctttgatat tctgggaact gctgcgcgcg cgttaggatt gcagtatttg
     4141 cttcttggaa cgcttggcgt aaagcttgat caggcgattg tttttttaga agcacaaaat
     4201 aatgatgctt gatttcatca accgctatac ggcttgcaat atcgccagcc tgatgccccc
     4261 ccattccatc cgcaacaata gccagaaaat tatggctgct atcaaagcca tcaattccaa
     4321 gacagtcctg attgacagat cggcaacgcc ccgtgtctga taacgaaccg atttccaaat
     4381 ggaaatacga tgccatttgt tgattctgct cagaatcagg agaatcttga aaaaattttt
     4441 tccatctgtc aaaaaactcc atcacgtcca gctataagta aaagattact tcatgatttc
     4501 aaggccatat tttatgggta aagcaagact aacttgagta ccttgctcgt ctcttatcaa
     4561 atcagtaaaa atgccaataa cctgtccttt actatcaaaa actggaccgc cacttgtacc
     4621 agctccagct gcattaatgg tcagttgata caataattca ttaacaagaa gtgatccatc
     4681 ttctttatga atcatatgct tttgaagaaa atcctcccta tttaggccct taaaaaggat
     4741 catttctaca ttttttccta ccgtatccaa tgttacgcct gctttattct cgattacctt
     4801 cccaataatt cctgttgtta ttgtaggctc gggtactttt cgattttgtc ttgttacgga
     4861 ggtatcagga gaaattatag gataacccat cacagtaact ggttcacctg tcgaaattga
     4921 tctaccactg ttgagttcaa catgaaccag ttttttcatc gcatcaacct tgatgagcgc
     4981 cacatccgca tttccagaaa tttttgttaa acgagcatta ctccgttgac ctaatcctgg
     5041 aaaagttact tctaaataat catgatatcc ttcaacatat tttcctgtct ggggtctccc
     5101 acctagatac tgtgtattag caggaatcca tttttctagc aatctgagat acttggtatc
     5161 cgcaatatta cctttgactt tgcaattctt accttcatcg ttgcagcgaa taacaaaccc
     5221 attttctaat tggctaatat catatgctac ttcccaagct gcagcgacat gagtattagt
     5281 taggataaat ccattcatat ccacaacaaa accgctgcct gtgttacttt taccaattgc
     5341 gacccctctc tcagtctcaa taccaaagct agagaataat tcatctctaa cattttcagt
     5401 aaataagagc ggttctacgt attcttttcc ctctcgttgg aaagaaacgt aggcaggaag
     5461 acatctcttt tcggatttag atttttgaca aactcgccca tgatagagct gttgacctga
     5521 ttcgacatct tttagcttcc agctcgtttc aatataaaca acagcggatt catatttctg
     5581 agatatttca gttgctgaca ttggggtatt tgacttcatt tcctccattt gcctggattg
     5641 ctcttcttta gcagcagcaa tattactttt caattgttct tcagtagcct gtaattgttt
     5701 ctgactttgc tgattttgat aaaaaaaata tccagcaact aagacaatga cagtgaagat
     5761 ccccgcactg acgttaatca ttttgcgccg tgctctacct tcagtctcgg caatcttgac
     5821 tcgcgcatca ctctcaaccc tatcgatgag tcgctctacc gtttcacggc ctaccccttg
     5881 cttctgatta gtgctaatgt tttggatgtc tgtcctactt aattgagact ctaccgtgct
     5941 actaacctga ctttcacgcg ttgatttagg aatgggaact tcttgcagtc gcgttgcttt
     6001 tgcagcaaca ggagggcgcg gatcaagatc aaattcaaat ttgggaccgt tcttgccaag
     6061 ttgcacaata tctccagctt gtaatatgac tggttttgca atcttttgct cattcacgaa
     6121 agtgccgttg ctactgccca aatccgtcag taagaattgg tcgtcttcaa ccagggtgat
     6181 ttttaaatgc gtccgactta ccagatcatc tttcagcgga tcatacgcaa ctttgcaatt
     6241 ggtatctcga ccaaaggtta ttgcatcgat aggaagctca aaaatctcag tttgatttgc
     6301 tttagaaccc gtcatatgct taacaattac tttttgcatg attgcattcc cttcatagaa
     6361 aaatcacgtt gtggttatca gatttcaatc tactttctta tatagatatc aaaaaagata
     6421 gtactttcac tcatctttca ttctatcaat tcaatttctt tttgccactt atcacagata
     6481 gttttttgtc attttaatgt agcaaaactt cacttcctgt gataaaaaac acatcggtaa
     6541 tgcagaattc acatgacgat aattttttcg tacatcgttt tgtgaatggt tccgcgactt
     6601 ttctcttcgc tctccttcaa tggcttcaac tcagctaaaa taatagtcgg tttaatcaat
     6661 tttgattttc tgcacaggac cactgattcc tgcagcctgt tttaacttaa tatcattaag
     6721 gttatctttt tcaccgaatg aggtgatgac aaagcagtaa ggtttcatgg cgattcctta
     6781 ttacaaaata gtgatttgat caaattttat agaaaaattc ttacacctta tgaagtatta
     6841 gtcgagacat tgtcattaac ttttttcaaa tcaattttct gggagtctat agaactttgt
     6901 tcgagcaatt ccgttagagc ctgtatggca ccgctaatac gcaacaatgt ttcttgtaaa
     6961 tgcagttctt gctgctgtaa ttcacgtaat ctgatttgac ctttttcgta ttcgccacgt
     7021 agtacttcaa ggcgttgatg aatttcttct ttcactgggc atccacttat tgtataaatt
     7081 taacgttaaa ctattgtaag atagattgtc gaactgctgt gattttcgaa tctcctggac
     7141 tgaaatatac caactcataa ggttccagac gatatccaat agtttttttt gtattattct
     7201 ctactatacg ccgtgtatca ttattagggg attcaagcat tgggctatcc ttatccggcg
     7261 tcacataatc cacaccgtca ataccgtcga gaagaacaaa cagatcggaa atataaactg
     7321 accgccccag tggccaaccc tgcttatcca aaccacccgt aagtggatga aaataatttt
     7381 caagctcctt cttaactaag ttttccataa ccacctcatc ttgatcagca aatatataca
     7441 caacaaaatg agcagaaatt ctaagaaaaa aaggtgatac aacatgcaaa cgggtcgtca
     7501 gcaaacaacg tggttttata tactctttaa tcgcagcaca cagcgatgat tctgcctcgg
     7561 gtggttgatc tggttttgta tttttggtaa gaattatcag actgacatga gccgacgcat
     7621 tgggtttgat aacagaagac tcgaggttat agcatggcaa acaaagcgct ctagctataa
     7681 tagcatcgcc tccaatacga aattccattg caattcgctt aaaatcttca ctcgtcacag
     7741 cgcgctgctc acttcgtaac tcaagtatgg caagacgaat ttcttcatca atcgaattgc
     7801 ccaaaatata ttttggacct cgcaataatt gaacaaaagc tcgtttattt tcatcagtaa
     7861 tacgattggc ccgatacaat agcatttcgg ttataaatgc aaacaactcg ataaacgtaa
     7921 taccaggatc tgaagtatta tgatcagtcc atgatggtgc aaagcttgga atcaatcgtt
     7981 ttccttcatc aactagatcg gagaaacttt tgtcatccaa attaggcagt ggtattgaga
     8041 tcggtaagtt tattttcata gcacgcttgt tattttgctc aagttgtaga gaatgttttt
     8101 tcaatttgag taatgggtat ccgctactta tctcaagaat cacgaatgga aaagattttc
     8161 tcaaggtaac agtaccgttc agttccatgt caaacacaac tgattgcggt gatatcgtaa
     8221 atagttctaa tgtcaaattg gcctgtggca ttacttgaat ggcctctata ccttttgata
     8281 ctgaaccaat actgtattgg aatggtaata gtactacctt tgtcccacta agttcgctgg
     8341 catcaataag tagtgtgtaa cccaagaatt ttaatcgagg ctcaccattc ccttctaaga
     8401 aaccgccatt actccaatca aactgcgtag catagcgaat caccccacca taaagcacct
     8461 caaataaatg ctcggctatt gctccagtat cgaagtataa gctgtaatgg gtaggcaata
     8521 agcgtacttt acttgcctta ttggtgggca atcggctggt agcgtcgcca ctaccaagac
     8581 gaaatgagcc agctatttgt gctaaagggg aagcgtccaa tgtaattgca tagccttcca
     8641 atcgcaattt gtttgtgtta ttaccgctca aatagccagg agggcgcaga tcgcctgaag
     8701 cgcgttgaat atccaagctc gaatcgtaat caatcgttcc tccaaagatc acctcgaatt
     8761 cggcgccatt tatcacgcca ctggcaagat ccaagccata gcgggccgga aataagcgca
     8821 cagtatgaga ttctcgggta gtcaaccaac gagttccatt acccagtcca aattggctaa
     8881 tcgcatcagc tagcggggtc gcatccagcg taatcggata accctccagt gtcagcgtag
     8941 tcgtctcgct gccagaaaaa aagccaggag cctccggatt gttgacgtcg aaagtttcat
     9001 cataatcaat agtccctttt ggagtaaccg aaaaaatgta accagatccg gagattgact
     9061 ggatcgtata cgcgccgggg agtagatgga atgtaacttt tttctgtatc gccacctcat
     9121 cactgggtgt tgcttctgaa atcaggaact gcggttgaaa aagtgggctg gaatctactg
     9181 caattgagaa acctatcagc ttcaattgcg tggtttcttt tccagcaaaa caccccccat
     9241 tgcttacatc caaaggttct tcgtagccta ttttaccttg cggtgtcacg gtataaacta
     9301 ccgatacaaa actcgcaggt gtttgaagat tgtacaatcc aggcaaaagt cggaatattt
     9361 gcttggtttt gccactcacc cagcctgaaa tgatataggg gtaagtgatc tgcggggtcg
     9421 atagcttctc atcagcgacg atctcaacag aatagccgag caactctaac cgggtggtcc
     9481 cgagtcctgc aaaacagccg ccgttgttgg catccaaatc ctcgtcgtaa tcgatcttgc
     9541 cctgaggggt aacaagataa attactgtcg catagccagc ggcgacctgg aggttgtatt
     9601 gtcccggcaa aagccggaat gtttgcaaag acttgcttcc caaacttggg ataatatagg
     9661 gataagcgat ctgctcggtc gacaatttct cagcaatgat ctctatggaa taaccgagca
     9721 attccaactg agtactttct ttccccgaaa aacaacctcc attcttgata tccagagtat
     9781 cttcgtaatc gatcttgccc tgaggggtaa cgcgataact tacattcgca tagttggcgg
     9841 caacctggag attatatagt cccggcagaa gccggaatgc ttgcggatcc ttggttttcc
     9901 gccattctcc ggtaggctgt gcgttgtgat aaatgatctc cgggctcgac aactggtttg
     9961 cagtaatctt gatcggatag ccgagcaatt ccagccgggt ggtcccgagt cctgtgaaac
    10021 agccgccgtt gt
//
LOCUS       DAOQLJ010000080        10006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2621_length_10006_cov_6.207014, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000080 DAOQLJ010000000
VERSION     DAOQLJ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 10006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10006
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2621_length_10006_cov_6.207014"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="PK522_06895"
     CDS             complement(<1..1272)
                     /locus_tag="PK522_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNC41497.1"
                     /translation="MKRNLLSQWLLQGQWHSHRLQMSMVMIAIALGIALGFSIQLINS
                     AAIHEFSSAIKILSGQSDLQIRAVQPTFDEMLYPKLAQREGVELASPVLEIQAAIPGQ
                     LDDTRNGMLKILGIDVFRAMAMTPDIVGVPAEDRNFDTLAIDAIFLSPAAMEWLKVAQ
                     GDSLSLRLGTKTVTLRVAGGLVRTNPGQRLAVMDIGAAQWHFDHVGLLSRVDLKLKQS
                     VNLEQFKAALEKELPSQYFVAETIDQEKRINNMSRAYRINLNVLALVALFTGIFLVFA
                     TQFLATLQRRPQLALLRVLGMTKKQILLQILKESSLLGLLGALLGIVLGYGFAISALY
                     FLGGDLGAGFFPDIQPQIQFDPWLTLLFLMLGIGVTLMGSITPAWEAANAHPAQALKS
                     GSEALSMTRLNKPWAATACIIIALIFTQLPPV"
     gene            complement(1278..1946)
                     /locus_tag="PK522_06900"
     CDS             complement(1278..1946)
                     /locus_tag="PK522_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC41498.1"
                     /translation="MEHPFALELRNIDKAFASKRQIFSRLSFSLRQGEYVAITGESGI
                     GKSTLLNLIAGLDFPDGGEILINGSLLPKDDNAATLLRRQHFGFIFQAFHILPHLNLL
                     QNVALPLLLNQFPIDRAQAMLTAVGLEGRAQDFPHQLSGGELQRVAIARALVHQPKLI
                     LADEPTGNLDPDTAREILYLLQSEIKASGAAAIIVTHSQFAAQTANRVLQLTASNLIS
                     VDHP"
     gene            2213..2800
                     /locus_tag="PK522_06905"
     CDS             2213..2800
                     /locus_tag="PK522_06905"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNC41499.1"
                     /translation="MQSLATRFSFITTQKHTTLKKGAQYLLAILMVANLMLFSRPAHS
                     SESVEYQVKAAFISNFIAFTAWPGDSNESIQLCLYGEDMFGTSLDQIAQKPNSKGKIK
                     VRRINDESTLTECQVVFIAKSNISNLSNILNTLREKPILSLADSPNAASQGVNINMNV
                     NSDGKVTFEINLATARLSKLDFSSKLLQLATHVYR"
     gene            2807..4837
                     /locus_tag="PK522_06910"
     CDS             2807..4837
                     /locus_tag="PK522_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNC41500.1"
                     /translation="MKIINRTLAFALMLLILLSTNAISGKPGDNQFLELSIEELMNVK
                     VTTVSRRAQKLSEVASAIFVITQDDIRRSGATSIPDALRMAPGVQVDRVSTGQWAVSI
                     RGFNGIYANKLQVLMDGRSVYSPIFSGVLWEQQDTLIEDIERIEVIRGPAATVWGANA
                     VNGVINIITKKAAETQGTLLSAGGGSFEHGFFGARYGGKINEETPFRVYAKGFTRDHM
                     ISPEGANRHDQWHSARGGFRLDHARGLDQFTLQGDIYFNSFGNSVDKNTSQLLALQTQ
                     GLRGNQEGGNIRFRWDRVYSERSSIMLQTYYDRNTHQLLPIGKSDAESFDIDFQHRLP
                     LFDKHDVIWGANYRLYNNKIFDTLLTSASPRERTHHQFSGFIRDDVALIPDRLLFSLG
                     TRLDHNDFSGLEVQPNARLMWTPNAQNSIWASISRAVRIPSRGENDITLNAGQLQSLP
                     GIPTLPIPISILMQGANNFGSEKLLAYELGYRHQFSQQASIDIAGFINDYSHLRDLSF
                     GALSFSTGLPRQLILPLLLNNRASALTYGFEVSADWKPTNRWRLQGNYSFLDISTSSN
                     ELLKTFDPTTGGAAKVNPQHQFSVRSNYDISDRLQFNLWLRYTSAIGFYNIPGFVTMD
                     TKLAFKPVKDVELFLVGQNLFSEYHREFVSDNIPSIPALIPRGIYVGARWQF"
     gene            5022..5408
                     /locus_tag="PK522_06915"
     CDS             5022..5408
                     /locus_tag="PK522_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41501.1"
                     /translation="MKTRHLLVLLAFVAQSFAVNAESTIAPSNHESLAQHHLSLVDEA
                     KVKLQEHKNNLEDYELHSYYYGRGGQDFRSHETANVRLYEEIIAENLKQAEFHQQMAE
                     AEKNQINKANINNFAAGEVSSTESTH"
     gene            5481..5822
                     /locus_tag="PK522_06920"
     CDS             5481..5822
                     /locus_tag="PK522_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41502.1"
                     /translation="MKKIFFSVLILTFLVTNLSVAAESENTATAKNKSPLSHEEIAQR
                     YDNKAKRMIDKIAEEKQMLDEYELHSYYYGREGQDFASHHEALLHKFEKAAKRYEELA
                     AAHREMARKTN"
     gene            complement(5918..6850)
                     /locus_tag="PK522_06925"
     CDS             complement(5918..6850)
                     /locus_tag="PK522_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HNC41503.1"
                     /translation="MIVKDKVSWVRLLFHFRGSSFMETWPRILTVTVTASVVTYIELY
                     YSIQAYTLTTTPFLLVGVALGIFLGFRNSASYDKFWEGRKLWGALVNTARSLTRQICH
                     MTDSRQSCETLQPFQAMFVKRIIAFAHALRCHLRNQEPFEEIKKFLPADDYAAVAVAK
                     HRPLIILQQLSRDLAIARDRRWLSEYNHPFLEAQLNEFSNILGGCERIKNTPIPFSYS
                     VLTHRIVAAYCLSLPFGLVETTGILTPVVVLMISYAFFELDAIGDEIESPFGLQPNDL
                     ALTAISRNIEINLLELINDADQPDIVKPVDDILI"
     gene            7144..9564
                     /locus_tag="PK522_06930"
     CDS             7144..9564
                     /locus_tag="PK522_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNC41504.1"
                     /translation="MNKKQLSERDICTKFITPALVRAGWDISSQIREEFPLTKGRIIV
                     RGKLHTRAERKRADYVLFYKSNIPIAIIEAKDNNHTVGDGMQQALGYAEMLQVPFVFS
                     SNGDGFLFHNKIAQDGIVERELALHEFPDAEMLWQWWVKHLGLNNQQNSLVTQDYYSD
                     GSNKNPRYYQLLAINKTIEAIARGQNRILLVMAAGTGKTYTAFQIIWRLWKSKTKKRI
                     LFLADRNILIDQTMTNDFKPFGSSMAKIQNRQVNKSYEIYLSLYQAVTGTEEEKNIYK
                     QFSPNFFDLIVIDECHRGSAATDSAWREILEYFSSATQIGLTATPKETKDVSNIDYFG
                     DPIYTYSLKQGIDDGFLAPYKVVRIDLDKDLTGWRPDKGMLDKHGFEIEDRIYNQQDF
                     DKTLVLEKRTQMVAKKISEFLKQTNRFDKTIVFCENIDHAERMRQALINENADLAGQN
                     NKYVMRITGDNEEGKAELDNFIFPESKYPVIATTSKLMTTGVDAQTCKLIVLDQRIQS
                     MTEFKQIIGRGTRINEDYGKYFFTILDFKKATELFADPDFDGDPVQIYEPKGTESPVP
                     PDISVEGASGESMIYSSSDTNPIWTGAAQTDPDNETYRVRRFTVANVEVKVVAERVQY
                     FDATGRLITESLKDYTRKAVAREYGSLDDFLRRWNSADRKQVIINELSEQGVFFDALA
                     DEIGQQSGKLFDPFDLLCHVAWDMPPLTRKERVEQVKKRNYFTRYGEQAQRVLQALLD
                     KYADEGMAQIEETQILTIAPFTGFGTPMEIIRAFGGLDQYQQAVHDLEDALYLPDRHS
                     RESGNPGG"
     gene            9566..9853
                     /locus_tag="PK522_06935"
     CDS             9566..9853
                     /locus_tag="PK522_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNC41505.1"
                     /translation="MKQPCVYILASQKNGTLYTGVTSDLIKRVWEHKHDQAEGFTSKY
                     AVHHLVYFEQHEDMNAAITREKQIKKWNRAWKLRLIEALNPNWNDLYETLI"
BASE COUNT         3038 a         2262 c         2091 g         2615 t
ORIGIN      
        1 aactggcgga agctgcgtaa agatcagtgc aataataatg caggccgttg ctgcccacgg
       61 tttattcaac cgggtcatcg aaagcgcttc actaccggat ttcagtgctt gagctgggtg
      121 agcattagcc gcttcccaag cgggtgtgat acttcccatt agcgttacac cgatgcctag
      181 cattaaaaat aggagtgtaa gccaaggatc aaattgaatt tggggttgaa tatcgggaaa
      241 gaaacccgct cccaaatccc cgcccaagaa atacaaggca gatattgcaa aaccatagcc
      301 caatacaatg cctaagagag cgcctaacaa tcccagcaaa ctactttctt ttaaaatttg
      361 tagcaaaatc tgcttttttg tcatgcctaa aacgcgcaac aaagccagtt gcggccttct
      421 ttgaagcgtg gcaagaaatt gtgtggcaaa taccaagaat atccctgtaa ataatgccac
      481 caatgccagc acattgagat taatgcggta agcacgggac atattgttga tgcgcttctc
      541 ctgatcgatg gtttctgcca caaagtactg agaaggcaat tctttttcta atgcggcctt
      601 gaattgctct aaattaaccg attgctttaa tttgagatca actcgcgata atagtccgac
      661 atgatcaaaa tgccactgtg ccgcaccgat atccataacg gcaaggcgct gccctggatt
      721 tgtgcgtacc agcccaccag ctactcttaa agtgactgtt ttcgtaccta agcgcaaact
      781 taaggaatcc ccttgcgcta cctttaacca ttccatggca gcaggtgata aaaagattgc
      841 atcgattgcc aaggtatcaa agttccgatc ttctgcaggc acaccgacga tatcaggtgt
      901 catggccatt gctcgaaaaa cgtcaatccc gagaattttc agcataccat ttcgcgtatc
      961 atccaattgg ccaggaattg cagcctgaat ctctagcact gggctagcca attcaacccc
     1021 ttcgcgctgc gcaagcttag gataaagcat ttcatcgaag gtaggctgaa ccgcgcgtat
     1081 ctgcagatca gattgtcctg ataaaatttt gatcgctgag gaaaattcat gaattgcagc
     1141 tgaattaatg agctgaatcg aaaaccccaa tgcaatgcct aaggcgatcg caatcattac
     1201 catcgacatt tgcaaccgat gactatgcca ctgcccttgt aataaccact gcgataacaa
     1261 attacgtttc atggcactca agggtgatca accgagatca aattagacgc agtcagttgc
     1321 aaaacgcgat tagcggtttg ggctgcaaac tgagaatgcg tgacaataat ggcagcagcg
     1381 ccactagcct tgatttcaga ctgaagcaag taaagaattt cgcgcgctgt atctggatca
     1441 agatttccag tgggttcatc cgctagaatt aatttaggtt ggtgaaccaa agcacgggca
     1501 attgcaaccc gctgcaactc acctcctgat agctggtgcg gaaaatcctg cgctcgacct
     1561 tctaaaccaa ccgcagtcag catcgcttga gcacggtcaa tcggaaattg attcaatagc
     1621 aagggaagcg ccacattctg taataggttc aaatggggaa ggatatgaaa cgcttgaaaa
     1681 ataaaaccaa aatgttgtct tctcagtaac gtcgcggcgt tgtcatcctt aggcaataag
     1741 cttccattaa tcaaaatctc ccctccatct gggaaatcta ggccagcgat caaattcaat
     1801 aacgttgatt taccgatacc cgattcaccg gtaattgcaa catattcacc ctgtcggagc
     1861 gagaaagata agcgagaaaa tatctgtctt ttactcgcaa aagccttatc aatgtttctt
     1921 aattcaagcg caaatggatg ttccatgtaa atttaaatgt taccaaagaa agcgttgtag
     1981 aaaagcgagg ttcgataggg cacttcatat caaaaataaa gcgataaatc gctatttaat
     2041 caaacgatca atcctgatga ctcgttttaa cataacaagg ttcaaattaa cgtaataagg
     2101 cacttactct aacttcacag agatagttaa ttatcaaggc cctgttttgc aattgttaat
     2161 ctcatttaac cgatgattta ttggcaatat ggctgagtaa tacgcttact taatgcaatc
     2221 actcgctact cgatttagct ttataactac tcagaagcac actacgctta agaaaggtgc
     2281 gcagtacttg ctcgccatac taatggttgc gaatctgatg ctattttctc gtccagctca
     2341 ttccagcgaa agcgttgaat atcaggtcaa agcagcattc atctcgaatt ttatcgcatt
     2401 taccgcgtgg ccaggtgatt ccaatgaaag cattcaacta tgtctttacg gtgaagatat
     2461 gtttggcaca agcctggatc aaatagcaca aaaacctaat agcaaaggaa aaatcaaagt
     2521 taggcgcatc aatgatgaaa gcacattaac tgaatgccaa gtcgttttca ttgccaagtc
     2581 caatataagc aatctatcga acatcctgaa taccctacga gagaagccta tattatcgct
     2641 ggcagatagc cctaatgccg catctcaggg agtcaacatc aatatgaatg tcaatagtga
     2701 tggaaaagtc actttcgaaa tcaatctagc aactgcgcgt ctttctaaac tcgattttag
     2761 ctcaaagttg ctacagctag cgacccatgt ttatcggtaa gaacctatga aaataataaa
     2821 tcgaaccctc gctttcgccc taatgctctt gattttattg agtactaacg ccatcagcgg
     2881 aaaacctggt gacaatcaat ttcttgagtt gagcattgaa gaattaatga atgtcaaagt
     2941 gacgactgtc tcaaggcgtg cacagaaatt aagtgaggta gcttcagcga tctttgtcat
     3001 cacgcaagac gatatccgcc gctcgggtgc aaccagcatt ccagacgcac tcagaatggc
     3061 ccctggcgtt caggtcgatc gtgtcagcac tggtcaatgg gcggttagta ttcgtggctt
     3121 taatggaatt tatgcgaaca aattacaagt tctgatggat ggtagaagtg tttatagccc
     3181 tattttttct ggggtattgt gggaacaaca agataccctg atcgaagaca tcgagcgcat
     3241 tgaggtgatt cgcggccctg cagcaacggt ttggggagcg aatgcagtca atggcgttat
     3301 caatatcatt accaagaaag cggctgagac tcaaggaacg ctcctttctg caggaggggg
     3361 tagttttgag cacggctttt ttggagcccg ttatggtgga aagatcaatg aagaaacccc
     3421 tttccgagtt tatgcaaaag ggttcacgcg tgaccatatg atttctcccg agggtgctaa
     3481 tcgccatgat caatggcact cagcgcgagg tggttttcgt ttagatcatg cgagaggact
     3541 cgatcaattt acgctacagg gcgatatcta ctttaattct tttggcaatt cagttgataa
     3601 aaatacttct caattgcttg cgctgcaaac tcaaggattg aggggaaatc aggaaggcgg
     3661 caatatccgg tttcgctggg atcgcgttta ttcagaacga tcgtctatca tgctgcaaac
     3721 ctattatgac cgaaacactc accaattgct gccaatagga aaatccgatg cagaaagctt
     3781 cgatattgat ttccaacatc gacttccgct ctttgataag cacgatgtga tttggggcgc
     3841 caattaccgc ttatataaca acaagatttt tgatacttta ttaacaagcg ctagtccgcg
     3901 agaacggacg caccaccagt ttagcggttt tattcgcgat gatgttgctt taatacctga
     3961 tcgtttatta ttttcactcg gaactcggct cgatcacaat gatttttcgg gattggaagt
     4021 tcaacccaat gcacgtttaa tgtggacacc caatgcgcag aattcgatat gggcatcaat
     4081 ttcacgtgca gtccggattc cttcgcgagg ggaaaatgat attactttga atgcgggtca
     4141 acttcaaagt ttgccaggaa tacctacttt gcctatccct atttctattc tcatgcaagg
     4201 agcaaacaat ttcggctcag aaaaactact tgcctatgag ttaggctatc gccaccagtt
     4261 ttcccaacaa gcatcgatcg atattgctgg attcatcaac gactatagcc acttacgcga
     4321 cttaagcttc ggggcgttat cctttagcac aggattacca aggcaattaa tattaccgct
     4381 gctacttaac aatcgcgcat ctgccctaac ctatggcttt gaagtctctg cagattggaa
     4441 acccacaaat cgctggcgac tccaaggaaa ttacagcttc ttagatatca gcacttcttc
     4501 gaacgaattg ctaaaaacat ttgacccaac gaccggtggc gcggctaaag ttaatccgca
     4561 acatcaattc tcagtgcgtt caaactatga tatttcagat aggttacagt ttaacctttg
     4621 gctgcgatat accagtgcaa tcggcttcta caatattcca ggtttcgtaa ccatggatac
     4681 taaactcgct ttcaaacccg tcaaagatgt tgaattattt ctagttggac aaaacttatt
     4741 cagtgaatat cacagagagt ttgtatcaga taatatccca tcgattccag cactcatccc
     4801 tcgtggcatc tacgttggtg cccgatggca gttctaacta cctacctgta agccactcca
     4861 tctgcatgtt taagcagtaa agcgatattg attagtatcg ctttgagttc taatggtttc
     4921 ccttaatgaa aaatcaggga tcgcccaata tacgcaagcc accctcttcg gtactatgac
     4981 gctatcgaaa cttttttcaa ttctgcataa ggaaatttaa catgaaaaca cgtcatctac
     5041 tcgttctact tgcttttgtt gcacaaagtt ttgctgttaa cgctgaatca acgattgctc
     5101 ctagcaatca tgaatcgctt gcacagcatc atttaagtct agtcgatgaa gcaaaagtaa
     5161 aacttcaaga gcacaaaaat aatcttgaag attatgagtt acatagctac tactatggta
     5221 gaggcggtca agattttaga tcacacgaaa cagccaatgt tcgtttgtat gaagagatca
     5281 ttgctgagaa cctgaaacaa gcagaatttc atcaacaaat ggctgaagca gaaaaaaatc
     5341 aaattaacaa agcaaacatt aataattttg ctgccggtga agtttctagt actgaatcta
     5401 ctcattagta acacatgaat catcccaaag aaggatgatt cattcattga aatgcgcgtt
     5461 aatttaagga gatcttgaac atgaaaaaaa ttttcttctc tgtactgata ttaacttttc
     5521 tagtaacaaa tctatcggtc gctgctgagt cggagaacac cgctactgct aagaataaat
     5581 ccccactgag tcatgaggaa attgctcagc gctatgataa taaagctaag agaatgatcg
     5641 acaaaattgc ggaagaaaaa caaatgttag atgagtatga acttcatagt tattactatg
     5701 gccgtgaagg acaagatttt gcgtcgcatc atgaagcctt attacataaa tttgagaaag
     5761 ctgctaaaag atatgaagaa ttagctgcgg cgcaccgtga aatggcaaga aaaacaaatt
     5821 agcgcatcag cgctgcaaag cagagagcgt tactaagttt aatataaaac ttgaaaaggg
     5881 taacgctcat ctctgtatca tctgtagtta tcaatcatca aatcaaaata tcatccacag
     5941 gcttaacgat atcgggttga tcagcatcat tgatcaactc cagcaagtta atttcaatat
     6001 tccgagaaat ggcagtcaag gcaagatcat tcggctgcaa cccaaatgga ctctcgattt
     6061 catcaccaat cgcatctaat tcaaaaaaag cgtaggaaat catcaagact acgaccggcg
     6121 tcagaattcc agtcgtctcg actaaaccaa aaggtagcga taaacaataa gctgcgacaa
     6181 ttcggtgagt caatactgag tagctgaaag ggattggagt gttcttaatg cgctcacacc
     6241 cccccaaaat attcgagaat tcattaagct gcgcttctaa aaacggatga ttatattcgc
     6301 tcaaccaacg acgatcgcgt gcaatggcta gatcacgact caattgctgc aaaatgatta
     6361 agggtcggtg ttttgcaaca gcaaccgcag cataatcatc tgcgggtaaa aattttttta
     6421 tctcctcaaa gggttcttga ttgcgtaaat gacagcgtag cgcatgtgca aatgcgatga
     6481 ttcgtttgac aaacatggct tgaaatggct gcaatgtttc gcaactctgt cttgaatcag
     6541 tcatgtgaca aatttggcgt gtcaagctac gtgcagtatt caccaatgcc ccccataact
     6601 tacgcccttc ccagaattta tcgtatgaag cactgttgcg aaaccctaga aaaataccta
     6661 acgcaacccc aaccaatagg aacggcgttg ttgtgagcgt gtaagcttga atactgtaat
     6721 agagctcaat gtaggtcaca accgatgctg tcacagtaac cgtgagtatt ctcggccacg
     6781 tttccataaa gctactgccg cgaaaatgaa acaacaagcg cacccaagac actttatctt
     6841 taacaatcac aactcaaatc tccttcgatt tcatgctcac atgttcatct tgatttagcc
     6901 tagcgcactc taaattagca aaaaccagcc atccttgttt tctttaaagc gtaacagaaa
     6961 accagtttta gcgtgatcgc taagctgttt catcgtttga gacctaataa agatttggat
     7021 ctcagcattt ttcaaagcat ctcaaccaag aaatctaagt tagaatgctg ggatatcttc
     7081 tgcttttaac ccttcttaat ctgaaatttt agttcatgga taaaaattga aaaaacggat
     7141 ctgatgaaca aaaaacaact ttccgaacgc gatatttgca ccaaattcat tacacctgcg
     7201 ctggtgcgtg cgggttggga tatctcatcg caaatcagag aagagtttcc gctcaccaag
     7261 gggcgcatca tcgtacgtgg taaattgcat actcgggctg aacgcaaacg cgcggactac
     7321 gtgttgttct ataaatcaaa tatcccaatc gccatcattg aagcaaagga taacaatcat
     7381 acagtcggtg atggcatgca acaggcgttg ggttatgctg aaatgctgca agttccgttt
     7441 gtgttcagct ccaacggcga cggtttctta tttcacaata aaatcgcaca agatggtatc
     7501 gtcgaacgcg agcttgcgtt gcacgaattt cccgatgcag aaatgctttg gcaatggtgg
     7561 gtaaaacacc taggcttgaa caaccaacaa aatagcctgg ttactcagga ctactacagc
     7621 gatggtagca ataaaaaccc acgttattac caattgctgg ctatcaataa aactatcgaa
     7681 gcaattgcac gtggacagaa ccgtatccta ctggtcatgg ctgccggtac cggcaagacc
     7741 tataccgcct ttcagatcat ctggcgactt tggaaatcca aaacgaaaaa acgtatcttg
     7801 tttcttgccg accgtaatat cttgattgat caaaccatga ctaacgattt caagcccttc
     7861 gggtcgtcta tggccaaaat tcagaatcgt caggtcaata aatcttacga aatttattta
     7921 tcgctttatc aagctgtaac tggcactgaa gaagagaaaa atatctataa gcaattcagt
     7981 cctaatttct ttgatctgat tgtgatcgat gaatgtcacc gcggcagcgc tgctactgat
     8041 tctgcttggc gtgagatttt ggagtatttt tcttccgcca cgcaaatcgg tttgacagct
     8101 acacccaagg aaaccaaaga cgtctccaat attgattact ttggtgaccc gatttatacc
     8161 tattcactca agcaaggtat cgacgatggt ttcctagctc cctataaagt cgtgcgtatc
     8221 gatctggata aagatctgac tggctggcga cctgataaag ggatgcttga taaacatgga
     8281 tttgaaattg aggatcgcat ttacaatcaa caagattttg acaaaacgct tgtgttggaa
     8341 aaacgcacac agatggtcgc gaaaaaaatc tctgaattcc tcaaacaaac caatcgcttc
     8401 gacaaaacca tcgtattctg tgaaaacatc gatcatgcag agcgcatgcg tcaggcgcta
     8461 atcaacgaaa atgctgatct tgcagggcag aacaacaaat acgtcatgcg tatcaccgga
     8521 gataacgaag aaggtaaagc cgaactcgac aatttcattt ttccggaaag caaatacccc
     8581 gtcattgcca ccacctcgaa actgatgaca acgggtgtcg atgctcaaac ttgcaaattg
     8641 atcgtactcg atcaacgcat tcaatccatg actgaattca agcaaatcat cggccgcggc
     8701 actcgcatca acgaagatta cggcaagtac tttttcacca ttctcgattt caaaaaagcc
     8761 accgagctat tcgctgatcc ggattttgac ggcgaccccg tgcagattta tgaacctaaa
     8821 ggaactgaat cccccgtgcc accggatatt tcagttgaag gagcaagcgg tgaaagtatg
     8881 atttactcga gttctgacac aaatccgatt tggaccggtg cggcgcaaac tgacccagac
     8941 aacgagactt atcgtgtccg ccgctttacc gttgccaatg tcgaggttaa agttgttgct
     9001 gagcgagtcc agtattttga cgcgacgggt cgattgatta ccgaatcact caaagattac
     9061 acccgcaagg cggtagccag agaatatggc tctctcgatg attttctacg ccgctggaac
     9121 agcgctgata gaaaacaggt catcattaat gaactatccg agcaaggcgt gttcttcgat
     9181 gcacttgccg atgagatcgg acaacaatcc ggcaagctgt tcgatccttt cgacttgctc
     9241 tgtcatgttg cgtgggacat gccaccgctc acgcgcaaag aacgagttga gcaggtaaaa
     9301 aaacgcaatt actttacccg ctatggtgaa caagctcagc gcgtgctgca agccctgctc
     9361 gataagtatg ccgatgaagg catggcacag atcgaagaaa cgcaaatcct caccattgcc
     9421 ccttttaccg gattcggcac accaatggaa atcatccgcg cctttggcgg tttggatcag
     9481 tatcagcagg ccgtgcatga cctagaagat gcgctttatc ttcctgaccg tcattcccgc
     9541 gaaagcggga atccaggagg gtgatatgaa gcaaccctgt gtctacattc tcgcaagcca
     9601 gaaaaacggt accctttata ccggcgtaac aagtgatctg attaagcgag tatgggaaca
     9661 caaacatgat caggcagaag ggtttaccag taaatatgcg gtacatcacc tggtttactt
     9721 tgaacagcat gaagacatga atgccgcgat tacccgcgaa aagcagatca aaaaatggaa
     9781 tcgggcttgg aagcttcgtc tgatcgaagc actgaatccg aattggaatg atttatacga
     9841 aaccttgatt tgaattttct cgtcatcctc ccacaggcgg ggacccagaa gacaaaattg
     9901 ctggattccc gctccccatc tgcacgagaa caggcatgta ggaatgacaa gatgaatcgc
     9961 cccgggtttt ccggagataa aatgatttga gaggaggagg aagaga
//
LOCUS       DAOQLJ010000081         9964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2639_length_9964_cov_5.189525, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000081 DAOQLJ010000000
VERSION     DAOQLJ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2639_length_9964_cov_5.189525"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..>1370
                     /locus_tag="PK522_06940"
     CDS             50..>1370
                     /locus_tag="PK522_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNC41506.1"
                     /translation="MANEPAKAASANPSNTTNTAAPAAPNAAPEKFNWGGVPPVTPMP
                     RPGLFVIPPAGPGYFSLWDLISGNKREAAPVAPYAPFGLLPTSGFDVDFRYLEKPDHE
                     KDVFDPFKRIKLADNWMLSFGGQFWYRYMHETDSRLNAGGQDNKYHLFRTRFHADLWY
                     RDQFRLFAEFIDARALGLDLPALTTDRNYTDILNLFADVKLGQLVDGPVYMRVGRQEL
                     LYGSQRLISPLEWVNTRRTFQGVKAFWRTPTFDLDAFWVRPMTIERNEFDNWDKGRNF
                     MGLWGTYKPMKGQLLDLYYLSLVDNRILNPFTVQQGNILQGDSVLHTLGGRVAGDYNR
                     FLYEFEGMYQFGRRSHLDISAFAVATGLGYHVPLPMNPQFWIRYDFASGDANSQDGRS
                     NTFNHLFPFGHYYMGFLDQVGRQNIHDFNAQFTLHPQPWFTFIAQYHR"
     assembly_gap    1371..1470
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1471..1770
                     /locus_tag="PK522_06945"
     CDS             <1471..1770
                     /locus_tag="PK522_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNC41507.1"
                     /translation="QFTLHPQPWFTFIAQYHRFYLANTRDFLYNAAGLATLRDPTGQS
                     GSHVGDEIDFRVNVHVSRHQDVLFGYSKLFAGDFLRAQRPGISPDFFYAQYVIRF"
     gene            1793..2116
                     /locus_tag="PK522_06950"
     CDS             1793..2116
                     /locus_tag="PK522_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfJ"
                     /protein_id="HNC41508.1"
                     /translation="MRRLLVSIIFSLFVFISTQGYAEQPAQPLPQNEGIVISTLDTTG
                     YTYMELGNGSKRFWIAAPTTQVKKGDHIRFVESMSMHNFTSKTLNKTFDRIIFVTSTQ
                     VKLAQ"
     gene            complement(2120..4234)
                     /gene="uvrB"
                     /locus_tag="PK522_06955"
     CDS             complement(2120..4234)
                     /gene="uvrB"
                     /locus_tag="PK522_06955"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNC41509.1"
                     /translation="MIITFPNSPFKLHQPFEPAGDQPEAISKLEAGIHAGLMFQTLLG
                     VTGSGKTFTIANTIARLGRPALIIAPNKTLAAQLYAEMREFFPENAIEYFVSYYDYYQ
                     PEAYVPSRDLFIEKDSSINEHIEQMRLSATKSLLERKDTIIVATVSCIYGIGDPVDYH
                     GMILHLREQEAISQRDIIKRLTEMQYQRNEFEFNRGTFRVRGDIIDVFPAENSELALR
                     VALFDDVVESISAFDPLTGRILQKIARFTVYPSSHYVTPRSTTLKAIETIKTELAERA
                     NYFYQNTKLVEAQRIEERTRFDLEMLNELGFCKGIENYSRHLSGRKPGEPPPTLIDYL
                     PREALLIIDESHVTVPQIGGMFRGDRSRKENLVTYGFRLPSALDNRPLKFEEFEQLMP
                     QTIFISATPADYENQHSGQVVEQVVRPTGLIDPQIIVRPVLSQVDDLMSEVNIRVKQG
                     ERVLVTTLTKRMSEDLTDYFSDHGIRVRYLHSDIDTVERVEIIRDLRLGKFDVLVGIN
                     LLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAARHIHGTAILYADRITNSMR
                     RAIDETERRRQKQLLFNEAHHITPKGVSKRIKDLIDGVYCSETAQNNLRAAQAEARYE
                     NMSESRMAQEIKRLEKQMLATAKNLEFEKAAELRDEIKKLKDKLFVGYVEPEINPSNT
                     NNAQPLAMNPKQGVIRRVKKQK"
     gene            4310..5503
                     /locus_tag="PK522_06960"
     CDS             4310..5503
                     /locus_tag="PK522_06960"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNC41510.1"
                     /translation="MELSKRAQAIKPSPTLAVTAKAAKLKSEGKDIIGLGAGEPDFDT
                     PQHIKAAAIAAIQAGFTKYTQVGGTPALKQAIVAKFKRENGIEYTTKQVTVSCGGKQS
                     FFNLVLSTINPGDEVIIPAPYWVSYPDIVLIAEGKPVFIDTGIADNFKITAAQLEAAI
                     TPKTKMFVINSPSNPSGSVYSLEELKALGQVLLKYPNILIATDDMYEHILLTEGKFSN
                     ILNACPELYPRTIVLNGVSKAYAMTGWRIGYCGGPETIITAMENIQSQSTSNPTSISQ
                     VAAETALNGDQSCMLPMIAAFRERNQFMTDKLNAITGFRCLLSGGAFYAFVDANQAIQ
                     SLTKQGLIATANDMDFSEYLLEKSGVAVVPGSAFGCNGYIRLSFATSMDNLTKAMDRL
                     KKLLA"
     gene            complement(5694..7466)
                     /gene="arsA"
                     /locus_tag="PK522_06965"
     CDS             complement(5694..7466)
                     /gene="arsA"
                     /locus_tag="PK522_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HNC41511.1"
                     /translation="MTKLKFLNQPPRFLFFTGKGGVGKTSLACATAIMLADSDLRVLL
                     VSTDPASNVGQVFGITIGNQVTAIAAVPRLAALEIDPQAAAQAYRDRIVGPVRGVLPD
                     TAVKGIEEQLSGACTTEIAAFDEFTALLTDSVLTADYDHIIFDTAPTGHTIRLLQLPG
                     AWSDFLAEGKGDASCLGPLAGLEKQRAQYKAAVDALADPERSRLVLVARAQQATLREV
                     ARTHEELTAIGLTKQFLVINGLLPEAETLQDPLAAAIFQREQKALAAMPEVLNQLPCD
                     HIVLKPFNLVGLTALRKLLIDALPDEGSKEFTHTVILFPSLSNLTDEIAKDGCGLIML
                     MGKGGVGKTTLAAAIAVNLAHRGLPVHLTTSDPAAHLSETLNGAMDNLAVDRIDPSVE
                     TERYRQHVLETKGAHLDAKGRALLEEDLRSPCTEEIAVFRAFSRIIREAGKKFVVMDT
                     APTGHTLLLLDATGAYHREIVKQMDPSIVHYSTPMMQLQNPKQTKIFLVTLAETIPVL
                     EAANLQADLRRAGIEPWAWIINNSVAATTVHSPLLGQRASNELTDIEVVSTAHAKRYA
                     VVPLLKEEPIGVQKLLDLSRSSSQ"
     gene            complement(7486..7836)
                     /gene="arsD"
                     /locus_tag="PK522_06970"
     CDS             complement(7486..7836)
                     /gene="arsD"
                     /locus_tag="PK522_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HNC41512.1"
                     /translation="MTIIHVFDPASCCSSGVCGSDVDQELVGFSADVDWVKQHDVAIE
                     RFNLAQQPMAFADNSTVKAFLERSGADVLPLILVDGEVALAGRYPQRSELARWAGIPL
                     EEKIEESSCCGGCC"
     gene            8237..8641
                     /locus_tag="PK522_06975"
     CDS             8237..8641
                     /locus_tag="PK522_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HNC41513.1"
                     /translation="MIGLLIISHENLGEEFVRCVTHILGERPSNLSCLAVQPNDDPSS
                     TITQAKKLLANLDQGEGVIIITDICGATPCNIASQLMIAGKVVCLAGINLAMLLRILS
                     YRHEPLDVVVSKALDGGKNSVMQILPELPHAS"
     gene            8631..8900
                     /locus_tag="PK522_06980"
     CDS             8631..8900
                     /locus_tag="PK522_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNC41514.1"
                     /translation="MLRNEVTILNKLGLHARASAKLTKLAGQFDSEVWITRNGRRVNA
                     KSIMGIMTLAANKGSILEIEISGKDEAAAMDAITALINNYFEEGE"
     gene            8897..>9964
                     /locus_tag="PK522_06985"
     CDS             8897..>9964
                     /locus_tag="PK522_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HNC41515.1"
                     /translation="MSFLLHGVGVSEGIAIGHAHLASPAALDVPHYLLPKNQIDHEVD
                     RLKHAFTTVRQELETLQASVSEGPAFAEFNAFLELHHMILDDPTLSDAAVNCIIQTQC
                     NAEWAIHQQMEVLIAQFEAIEDAYLRERKADVTQVVERILKVLLGMPGYIPPPLKHDG
                     MSILVAHDLSPADVMQYKQHQFAAFLTDLGGVTSHTAIVARSLNIPSIVAMQHARQLI
                     YNNDLLIVDGSRGIVIVNPDKYILAEYRLKKNQLELEKRKLKRLRTLPTITLDGSIIE
                     LHANIELPQDIEPVQESGATGIGLFRSEFLFLNRDDLPNEEEQFAAYRFVAERMQGMP
                     VTIRTFDLGADKNLKQDIHRAA"
BASE COUNT         2658 a         2355 c         2166 g         2685 t
ORIGIN      
        1 gataaatgga tttttaacga tcgcaaccat aagctgcacc tttcagtcgt tggcgaacga
       61 gcccgctaaa gcagcatcag cgaatccatc aaatacaacc aatacagcgg cacccgctgc
      121 accgaatgca gcacctgaaa agtttaattg gggaggcgtt ccaccggtaa cgccaatgcc
      181 acgccccgga ttatttgtga ttccaccggc aggacctggt tatttttcat tatgggatct
      241 catctcaggc aataaacgtg aagctgcgcc ggtagcacct tatgcaccct ttggactgct
      301 acctacttct gggtttgatg ttgattttcg ctatctggaa aaaccagatc atgaaaagga
      361 tgtcttcgat ccgttcaaac gcataaaact ggctgataac tggatgctgt cattcggtgg
      421 gcaattctgg tatcgctaca tgcacgaaac cgatagccgc ttaaatgcgg gggggcagga
      481 taataaatat catcttttcc gtactcgttt ccatgctgac ctttggtatc gcgatcaatt
      541 tagattattt gctgaattca tcgatgcgcg cgcgttgggt cttgatttac ctgcgctgac
      601 gacagatcgg aattatacgg atatactcaa tttatttgct gacgttaaac tcggccaact
      661 cgtcgatgga ccagtataca tgcgggttgg gcgtcaagaa ttactgtatg gttcgcaacg
      721 cttgatttca ccgctcgagt gggttaatac gcgccgtacc ttccaagggg ttaaagcatt
      781 ctggcgtacc ccaacttttg atttagacgc attctgggtt cgtccgatga cgatcgaacg
      841 taatgaattc gataattggg ataaaggtag gaatttcatg ggtctttggg gaacctataa
      901 accaatgaaa gggcagttgc ttgatctcta ttacttgagt ttggtcgata accgtatcct
      961 gaatcccttc accgtgcaac aagggaatat tctgcaaggg gattctgtgc tgcataccct
     1021 tggtggacgt gttgccggtg attacaaccg ctttctttac gagtttgaag ggatgtatca
     1081 gtttggtcgt cgttcacacc tcgatatatc ggcttttgca gttgcaactg ggttaggtta
     1141 ccacgtgccg ttaccgatga atcctcaatt ctggattcgt tatgattttg cttcaggtga
     1201 tgcaaacagc caagatggaa gatcgaatac ctttaatcat ttattcccat ttgggcatta
     1261 ttacatgggt ttcttggatc aagtaggtcg tcaaaacatt cacgacttta atgcgcaatt
     1321 taccttacat ccgcaaccgt ggtttacctt catcgcacaa tatcatcgct nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caatttacct tacatccgca accgtggttt
     1501 accttcatcg cacaatatca tcgcttctat ttagcgaata cgcgtgattt cttatataac
     1561 gcggcagggc ttgcaacatt gcgtgatcca actgggcaat caggtagcca tgtgggagat
     1621 gaaatagact tccgggttaa tgtccacgtg agtcgtcatc aagatgtctt gtttggttat
     1681 tccaaactat ttgcaggtga ttttttaaga gcgcaaagac caggcatttc acctgatttc
     1741 ttctacgcac aatacgttat tcgtttctaa tacgaggata ttgttaatcg ttatgcgtcg
     1801 cctacttgtt agtattattt tttcattgtt cgtgttcatt tctacacaag ggtatgctga
     1861 gcaacctgct cagcccttac ctcagaatga aggcattgtg atatcaacgc ttgataccac
     1921 aggatacact tatatggagc tgggaaatgg gagcaagcgt ttttggatag cagcccccac
     1981 gacccaggta aaaaaaggcg accatattcg ctttgtcgag agtatgtcga tgcataattt
     2041 caccagtaaa acattgaata aaacgtttga tcgcatcatt tttgtcacgt ccactcaagt
     2101 taagctggcg caataacggt tatttctgtt ttttgactcg ccgtataacc ccttgcttag
     2161 ggttcattgc caggggttgt gcattattgg tattcgatgg gtttatttca ggttcgacat
     2221 aacccacaaa caacttgtct tttagctttt tgatttcgtc gcgtaattca gccgctttct
     2281 caaattcaag attctttgct gtggctagca tctgtttttc gagacgcttg atctcttgcg
     2341 ccattcggga ttcactcata ttctcgtaac gtgcttctgc ttgtgccgca cggagattat
     2401 tttgcgctgt ttcactgcaa taaacgccat cgatcaaatc tttgatgcgt ttactgaccc
     2461 ctttgggcgt aatgtgatgc gcttcattaa ataataattg cttctgacgc ctccgttccg
     2521 tttcatcgat cgcacgacgc atcgaattag taatacgatc cgcatacaat atcgccgtac
     2581 catgaatatg gcgtgccgcc ctcccaatgg tttgaatcaa agaacgctca gaacgtaaaa
     2641 atccttcttt atctgcatca agaatagcca ccagcgacac ttcgggaata tccaatcctt
     2701 cccgcaataa gttaataccc acaagcacat caaattttcc taatcttaga tcgcgaatga
     2761 tctcgacgcg ctcaaccgta tcaatatcag aatgaagata acgcactctg atgccatgat
     2821 cggagaaata atcggttaaa tcttctgaca tacgcttagt tagcgttgtg acgagaacac
     2881 gttccccttg cttcactcgg atattcactt ccgacatcag atcatcgacc tggctcagta
     2941 ccggtcgtac gataatctgt ggatcgatca agcccgttgg ccgcaccact tgttccacca
     3001 cctgaccact atgttgattt tcgtaatcag cgggcgttgc agaaataaaa atcgtctgcg
     3061 gcattagctg ttcaaactct tcaaatttaa gcggacgatt atcaagcgca gaaggcaatc
     3121 gaaaaccata cgtcaccaga ttttctttgc gggagcgatc gcctcgaaac atgccaccaa
     3181 tttgcggcac tgtcacatga ctttcatcga taatcaaaag cgcctcacga ggcagataat
     3241 cgattaaggt tggcggtggt tctcccggct ttctccccga caaatggcga gaataattct
     3301 cgatcccttt acaaaacccg agttcattca acatttcgag atcaaagcgg gttcgctcct
     3361 caatgcgctg tgcttccact aacttggtgt tctgataaaa gtaattagcg cgttccgcta
     3421 attcagtttt tatggtttcg atcgctttca aagtggtact ccgcggcgtc acataatgac
     3481 tggaaggata caccgtaaag cgcgcaatct tctgcaagat acgccctgtc aggggatcaa
     3541 aagctgaaat actttccacc acatcatcaa acaacgcaac acgaagtgca agttcagaat
     3601 tttctgcggg gaaaacatcg atgatatcgc cacgcactct aaacgtgccg cgattgaatt
     3661 caaactcatt gcgctggtat tgcatttccg tcaagcgctt aatgatatcg cgttgcgaga
     3721 tcgcttcttg ttcgcgcaaa tggagaatca tgccatgata gtcgacagga tcgccaatcc
     3781 cgtaaatgca tgaaactgtt gccacaataa tagtatcttt acgctctaat aaggatttcg
     3841 ttgcggaaag ccgcatctgc tcgatgtgtt cattgatgct ggaatctttc tcgatgaaca
     3901 aatcacgaga aggtacatag gcttccggtt gataataatc ataataagaa acgaaatact
     3961 cgatggcatt ttctggaaaa aattcacgca tttcagcgta cagttgcgca gccagtgttt
     4021 tgttcggcgc gatgatgagc gcgggcctcc ctaaacgcgc aatggtattg gcaatagtaa
     4081 aggttttgcc tgacccagtg acccccagca acgtttgaaa catcagcccc gcatggattc
     4141 ccgcttctaa tttgctgatc gcctccggct gatcgcccgc aggttcaaat ggttgatgta
     4201 gcttaaatgg actgttggga aaggtaataa tcacaggtat aatttcgtga atcgcaaact
     4261 aatttctaaa ttttaacacg gcagcatcaa ctcaatcagg agcacagcag tggaactttc
     4321 aaaacgggcc caagcaatca aaccttcccc tacccttgca gtcacagcca aggcggctaa
     4381 actcaagtca gaaggtaaag atattatagg actgggcgct ggggaacctg attttgatac
     4441 gccacaacac atcaaagccg ctgcaattgc agccattcag gccggattca ccaagtatac
     4501 ccaagttggg ggaacaccgg cactcaaaca agcgattgtc gcaaaattca agcgagaaaa
     4561 tggaatagaa tacaccacaa aacaagtcac cgtgtcctgc ggtgggaaac aaagtttttt
     4621 taatttggtt ctatccacta tcaacccggg ggacgaagtc attattcccg cgccttactg
     4681 ggtatcctat cctgacatcg ttctcattgc ggaaggcaaa ccggttttta tcgataccgg
     4741 aattgctgat aattttaaaa ttaccgccgc acagcttgaa gcagcgatta ccccaaaaac
     4801 caagatgttc gtgatcaaca gccccagcaa tccatccggc agtgtctata gccttgagga
     4861 actgaaagca ttagggcagg tactgctgaa ataccctaat atactgattg ccaccgatga
     4921 tatgtacgaa catatcttat taacggaagg taaattttca aatattttga atgcttgccc
     4981 tgagttatac ccacgaacca tcgttctgaa tggcgtttcc aaagcctatg ccatgaccgg
     5041 ttggcgcatt ggttattgtg gcgggcctga aacgatcatc accgccatgg aaaatatcca
     5101 atcgcagagt acctctaacc caacgtcgat ctctcaagtt gcagcagaaa ccgcgctcaa
     5161 tggcgatcaa tcctgcatgt tacccatgat cgcagctttc cgcgaacgca atcagttcat
     5221 gaccgataaa ctcaatgcga ttactgggtt tcgatgctta ctctccggcg gtgctttcta
     5281 tgcatttgtg gatgccaatc aggccattca aagtttaaca aaacaaggcc tgatagcgac
     5341 tgccaacgac atggatttca gcgaatattt actggaaaaa tcgggggtgg ctgtagtgcc
     5401 aggatccgcc tttgggtgta atggctacat ccggctttcg tttgcaacct cgatggataa
     5461 tctcactaaa gcgatggatc gcctcaagaa attgctagcg tgagtgcgga ttcggcatac
     5521 attgatttat gcactaccat agcgttaaga aatatgcttg ctcggtgaat tcgaactcaa
     5581 cacttcaaga tgttcagcaa gccatacttt aataatgggc tgccgcggcc tcatgcacgc
     5641 caattcaaaa gcacggtaaa aaacagcgac accacttttc tatccatgaa ttgttattgg
     5701 ctactgctcc ttgataaatc caataacttc tgcacgccga tcggctcttc tttgagtaat
     5761 ggcacaaccg cataacgttt tgcatgggca gttgatacca cttcgatatc agtaagttca
     5821 ttgctggcgc gctggcccaa taagggtgaa tgaacagttg ttgcagcaac actgttatta
     5881 atgatccacg cccatggctc aataccagca cggcgtaaat cagcttgtag gttggcagct
     5941 tccagtaccg gtatcgtttc cgctaatgta accaggaata ttttggtttg cttagggttt
     6001 tgtagttgca tcattggtgt tgagtagtgc acgatggaag ggtccatttg tttgacaatt
     6061 tcgcggtgat aagcgccggt cgcatccagt aataataagg tgtggccggt tggtgcagtg
     6121 tccatcacga caaacttctt acccgcttcg cgaattattc ttgagaatgc ccgaaatacc
     6181 gcaatttctt cggtacaagg cgagcgcaaa tcttcttcca ataaagccct gcctttagca
     6241 tctagatgtg cgcccttagt ttccaggaca tgctgacggt agcgttcagt ctccacagag
     6301 ggatcaatcc gatctaccgc tagattatcc attgctccat tgagcgtttc actcaaatgt
     6361 gctgcaggat ctgaagtagt caaatgtact ggcaaacctc gatgtgccag attcacggca
     6421 atagcagcag ccagtgtggt tttgccaaca ccgcctttgc ccatgagcat gattaaaccg
     6481 cacccatctt tggcaatttc atcagtcaag ttagataagc tggggaatag gattacagta
     6541 tgcgtaaact ctttgctgcc ttcgtcaggc aaagcatcaa tcagcaattt ccgtaaggca
     6601 gtcaatccga ctaggttaaa cggtttaagt acaatatggt cgcaaggtag ttggttgaga
     6661 acttccggca tggctgccag cgctttttgt tcgcgctgaa aaattgcagc agctaaaggg
     6721 tcctgtaatg tttcggcttc tggtaacaag ccattgatga ccagaaactg tttcgtcaag
     6781 ccaatagctg ttaattcttc atgcgtccgc gctacctcgc gtagcgtagc ttgttgcgca
     6841 cgagccacca ataccaatcg actccgctcc gggtcagcaa gagcatctac cgctgctttg
     6901 tattgggcgc gctgtttttc cagaccagct aatgggccaa gacaagaggc atcacccttg
     6961 ccttccgcaa gaaaatcact ccatgcgccg ggcaattgca gcagccgaat ggtgtgaccg
     7021 gtcggcgcgg tatcgaagat aatatgatcg tagtccgcag tcagcaccga gtccgtcaat
     7081 agtgcggtaa attcatcgaa ggcagctatc tcggtcgtgc atgcgccgga taattgctct
     7141 tcgatacctt tgactgctgt atccggcaac acgccacgca ccggaccgac gatgcggtcg
     7201 cggtaagctt gggctgccgc ttgcgggtca atttccaagg cggctaaacg gggtactgcg
     7261 gcaatcgccg taacctgatt tccaatcgtg ataccgaata cctgacctac gttagaggct
     7321 ggatcagtgc tgaccaataa tactcgcaaa tcggaatcag ccagcattat cgctgtggcg
     7381 caagccaatg atgtttttcc gactccgcct ttgccggtaa aaaatagaaa acgcggtggc
     7441 tgattgagaa atttgagttt ggtcacgagc aaacctgagc ttgcttcagc aacaaccgcc
     7501 gcaacaactc gattcttcaa ttttttcttc aagaggaatc cccgcccaac gcgcgagttc
     7561 gctacgctgc ggatagcggc ctgccagtgc cacttcccca tctacaagaa ttaaaggtag
     7621 cacatcagca ccagatcgct ccaagaatgc ttttaccgta gaattatccg caaaggccat
     7681 gggttgctgt gctaaattaa aacgctcgat tgcaacgtcg tgctgcttga cccaatctac
     7741 atcagccgaa aaaccgacca gttcctggtc tacgtcagaa ccgcaaacgc ctgagctaca
     7801 gcatgaggca gggtcaaata catggataat tgtcattttg attctcctat aaagttaaac
     7861 taacaaggtt gcgatacaat ttaattctac ttttagctat tagcgcatag caccgcaaag
     7921 ctgacctcag attttaatcg ctccagaact cctgggtaaa tatcgcattt attttttcat
     7981 tacaccatcc cctcaaaaaa cagcgtctct tacaaacttg ggacgattca aatttcatga
     8041 actccatatt tgacaataca cctcaaacac caagtaaagt gcatttatat ggccctaaac
     8101 tcgctgatat attttgtaag tcttgagtca tataaagatt tttgtagcct ctcccaaaat
     8161 aacctaacat gactacttac gaactcgcgt gttaatgtta ttttattcac tatttcaaac
     8221 taccaagaag aagataatga ttggcctatt aattatttct catgagaatc tgggagagga
     8281 atttgttcgg tgtgtaacgc atattttagg agagcgacct agcaacttga gttgcctcgc
     8341 tgttcaaccg aatgacgatc ccagttcaac gataacccaa gcaaaaaaat tgcttgctaa
     8401 tttagatcaa ggagaaggtg tcattatcat caccgatatc tgtggcgcta caccgtgtaa
     8461 tattgccagt cagctcatga tcgctggaaa agtcgtttgt ttggctggaa tcaatttagc
     8521 gatgctatta aggattttga gctatcgcca cgaacccctc gacgtggttg tatccaaagc
     8581 gctcgatggc gggaaaaata gcgtcatgca aattttaccg gagttaccgc atgcttcgta
     8641 acgaagtcac gattctcaac aaactcggac tccatgcacg tgcttcggca aaactcacca
     8701 aattagcagg gcaattcgat agcgaagtat ggataacccg caatggtcgc cgcgttaatg
     8761 caaaaagtat tatgggtatc atgacattag cagctaataa aggttcgata ctggaaatcg
     8821 aaatttcagg taaggatgaa gcggcggcaa tggatgcgat caccgccctc attaacaatt
     8881 attttgagga aggtgaatga gctttctatt acatggggta ggtgtttctg aaggcatcgc
     8941 gatcggacat gcacatctcg cctcccctgc ggcattggat gttcctcatt atttattgcc
     9001 taaaaaccaa atcgaccatg aagtggatcg cctcaaacat gcattcacga ccgttcgtca
     9061 agaattggaa acgctgcaag cttctgtaag cgagggacct gcctttgctg aattcaatgc
     9121 attccttgaa ttgcatcata tgattctgga tgatccgacg ttgtccgatg cggctgttaa
     9181 ttgcattatc caaacccagt gcaacgcaga atgggcgatt catcagcaga tggaagtact
     9241 cattgcgcaa ttcgaagcca tcgaagacgc ctatctccgt gaacgaaaag cagacgttac
     9301 gcaagtggtt gagcgtatct taaaagtgtt gctcgggatg cccggttata tcccccctcc
     9361 gcttaagcat gatggaatga gtattttagt tgcacatgac ctaagtcctg cggatgtcat
     9421 gcaatataaa caacatcaat ttgctgcctt tctgacagat ctgggtggag taacctctca
     9481 tactgccatc gttgcgcgca gcttaaatat tccatcgatc gttgcgatgc aacatgcacg
     9541 ccaattgatc tacaacaatg atctgttaat tgttgatggt agtcgtggaa tcgtgattgt
     9601 caatccagat aagtacattc tggctgaata tcgcctcaag aaaaatcaac tcgaattaga
     9661 aaaacgtaag cttaaacgac ttagaacgtt gccaaccatc acgcttgatg gcagtatcat
     9721 cgagttacat gcaaacattg aacttcctca agatattgaa ccggtgcaag aaagtggcgc
     9781 gaccggtatc ggtttatttc gcagcgaatt tctttttcta aaccgtgacg acctccccaa
     9841 tgaggaggaa caatttgcag cttatcgctt tgttgctgag cgcatgcaag gaatgcccgt
     9901 tacaatccga acatttgact tgggcgctga caaaaacctc aagcaggaca tccatcgtgc
     9961 tgca
//
LOCUS       DAOQLJ010000082         9881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2674_length_9881_cov_6.172807, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000082 DAOQLJ010000000
VERSION     DAOQLJ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9881
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2674_length_9881_cov_6.172807"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2102)
                     /gene="infB"
                     /locus_tag="PK522_06990"
     CDS             complement(<1..2102)
                     /gene="infB"
                     /locus_tag="PK522_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNC41516.1"
                     /translation="MSQITVEQFANELGMLPDALLEQLQTAGVSKQSGEEIITEGDKK
                     QLLEYLRKSHGAAETTKKKIALTRKKTTEIKKSDVAGKQRTIEVKVRKTRVYTKPVEE
                     TVEQSEDKETKAAIAPIEVSVSVPETSSVRKPIISADELAQREEEARRHAKLIELQEI
                     EFRKKAEKKIQPKVSEVIKEEPKQEVPVEKVTNTAELASQPTAQQPKSTITVKHEEKP
                     EKKKKQVKQQNLLSEETGKRREIKKHDLNQADGWRIRKTNKNAKTSSNESAELHRFTE
                     PTEPIKYEVMVPETISVAALAQKMSVKAAEVIRVLMKMGIMVTINQMLDQDTAMIVVE
                     ELGHIAKYAAPDNPESYLEEEKASQSDAIAEPRAPVVTVMGHVDHGKTSLLDYIRRTR
                     VAGGEAGGITQHIGAYHVETAHGMITFLDTPGHEAFTAMRARGAKITDIVVLVVAADD
                     GVMPQTIEAIHHAKAAKIPIVVAVNKMDKPEANFERIKQELVTHGVVAEDWGGEVIFV
                     GVSAKTGLGIDELLESILLQAEVLELKAVKAAPAKGVVIESRLDKGRGPVATILIQSG
                     TLKRGDVLLTGAAFGKVRAMLDENGNPIESASMSIPVEIQGLSDVAVAGETVLALEDE
                     RRAREIALFRQGKFRDVRLDQRQVAKMEEVFNQKDDIKILNLIIKADAQGSAEALAYA
                     LQKLATDEVKINIIHSGVG"
     gene            complement(2211..3683)
                     /gene="nusA"
                     /locus_tag="PK522_06995"
     CDS             complement(2211..3683)
                     /gene="nusA"
                     /locus_tag="PK522_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNC41517.1"
                     /translation="MSREILLLVDALAHEKNVSKAVVFTALEMALASATKKHFREDIE
                     VQVRIERDTGNYRCFQSKQVVPDDALENPFRQVILSEALKLNAEIKLGDFIEEPIETL
                     EFDRIGAQAAKQVIFQKIRDAEREQNLNDFLDRKEYLISGTIKRMERGNAIVESGRIE
                     AILPRDQMIPKENLRVGDRVRGYLQRVDRTSRGPSLVISRISPEFLIKLFQLEVPEIE
                     EKLLEIKAAARDPGSRAKIAVKSNDPRIDPIGTCVGMRGSRVQSVITELTGERIDIIL
                     WSEDPATYVVNALAPAEISSIVVDEDKHAMDIVVNDENLAQAIGRGGQNVRLASELTG
                     WVLNIMTEEESQKRNEEETSVISKLFIDKLDVDEEVAAILVEEGFSTLEEIAYVPLEE
                     MLEIEALDEETITELRHRASNALLTAAIATEERIERVSEDLLALEGMDIQLVRELAAK
                     GINTQEELADLAADDLVEMTGIDIERANQIIIKAREPWFV"
     gene            complement(3711..4139)
                     /gene="rimP"
                     /locus_tag="PK522_07000"
     CDS             complement(3711..4139)
                     /gene="rimP"
                     /locus_tag="PK522_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNC41518.1"
                     /translation="MALYELLDSTLNNMGYELVDVEFSPSGKLLRVFVDKEGGISIDD
                     CVAISNHLSRLLVVEQIDYGRLEVSSPGLDRPLRKERDFVRFLGRLVKVKSHIALQGQ
                     RNFVGILREVSDGKLGLEIEGKILHIELNNLDKARLVPEL"
     gene            complement(4371..6551)
                     /locus_tag="PK522_07005"
     CDS             complement(4371..6551)
                     /locus_tag="PK522_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HNC41519.1"
                     /translation="MLHFSLFNRTPVILQTEAAECGLACLAMIASFHGHQINLNGLRA
                     QHAASLRGSTLADLIQIAEQLKLASRPLRLDLDQLAELKLPCILHWNFNHFVVLTRVQ
                     RDRVVLHDPASGECELTLAEFSKHFTGIALELTPTSDFVTRNEIQRVKLRSLIGRLRG
                     LTGAISQILILALILQVFAILAPFYMQWVVDHALVAYDHDLVTILGVGFLLLAVVQVS
                     VTALRSWVLMVLSTTLNLQMMTNLFHHLIRLPMQWFNKRHIGDIVSRFDSLNVIQRTL
                     TTGFLEALIDGVMVLITLGMMLLYSVKLALIAMAAAGLYGLLRLVLYRPLRLATEDHI
                     VRGAKQQSHFLETVRGIQSIKLFAYEASRAANWQNLVIDQFNASIRTQRFNILYQGLN
                     GLLFGLENIITIWIGAMLILDTATGTGFSIGMLFAFIAYKTQFVQRISALIEKGLELR
                     MLSLHTERVGDVALTPIEQVNELHPISATPLTGRIEVKHLTFRHSETDPLVLQDIQFS
                     VQAGESVAIVGPSGCGKTTLVKLMLGLLQPTGGQIEIDGVPLSRMSLARYRQAVASVM
                     QDDQLFAGSIAENICFFDATPDFERVMASARLAAIHDDIMAMPMQFNTLVGDMGAVLS
                     GGQKQRVLLARALYRQPAILFLDEATSHLDIHCEQNVNQAIHTLKLTRIIVAHRPETI
                     ASADRVIQLVAGRIVSDTIVPAMANYHSESQMNELESQYAIAAN"
     gene            complement(6554..7801)
                     /locus_tag="PK522_07010"
     CDS             complement(6554..7801)
                     /locus_tag="PK522_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNC41520.1"
                     /translation="MARPLFRSEAIAHQQDTRQGEVLIARSLTIRLFVWVALIFTLGL
                     LAFAYWGEYTRKTHVVGYLSPTTGMIRIYSPQLGTIQEKHVREGQVVKQGDPLLTISS
                     ERTTSNAREAQAAMLTELKQRQVSLRFEQGKQSEIDALTGTSINARIRGLENEIRETQ
                     AQLALQSSRVASAERMVVRYQQLVAERFMSEVALQEKQEALLDQRNQLVQIKRTIASL
                     NRELNTARSELSASGLKKVNNRAAIERQISELEQQLTETDLRRSIVLTAPTEGIVTTL
                     LAEMGQMAQPNLPLLSILPAGAQLQAQLLVPTHSVGFIKPGQMVSLRYQAFPYQRFGH
                     HLGEIIEVSRAVIQPNEANLPVSIQESVYRATVRLPQQQIIAYGELLPLQPEMILDAD
                     IHLDRRRLIEWVFDPLLSITGRI"
     gene            complement(7889..8059)
                     /locus_tag="PK522_07015"
     CDS             complement(7889..8059)
                     /locus_tag="PK522_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41521.1"
                     /translation="MNEFDFNESVSLDEIEVHTLTADELSLIAGGMMKMDEGVNVGKL
                     AAYKEMYGDMWF"
     gene            complement(8182..9219)
                     /locus_tag="PK522_07020"
     CDS             complement(8182..9219)
                     /locus_tag="PK522_07020"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNC41522.1"
                     /translation="MGPTGLSRILQIHPSRQCNLSCLHCYSSSSPHEKEHLTLKLLCD
                     AISDAAAEGFNYVSLSGGEPMLYKPLVELLEHAHQLGMRTAVTTNGMLLTEQKIAQLR
                     EVVDVLAISIDGVPSSHNRIRGSQIAFDTMRSRLPLLRAEGLTFGFIFTLTQYNLDEL
                     NWVREFAMGEGAKLLQIHPLEEVGNAATRLKGNSPDATESAYAWILAQQKIDSDIKIQ
                     VDLAFSEVIKQHPELVFAGDCANLETKPFGELISPLVIEADGTVSPIQHGFSRNFSVG
                     NLKAFPLSTLLEDWREYGLPAFHALCREVYEEIANTSSPLFLNWNSLIGKRAESALFN
                     PQDQIRKFMVV"
     gene            complement(9234..9452)
                     /locus_tag="PK522_07025"
     CDS             complement(9234..9452)
                     /locus_tag="PK522_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41523.1"
                     /translation="MSQEMRGSEKLKATCQTMASLTPEQIAQVAGGFSWSGQISPYWL
                     INGIPFDPHFIKFQPLQLNEFNINQQQF"
BASE COUNT         2820 a         2517 c         1770 g         2774 t
ORIGIN      
        1 cctacaccac tatgaatgat attaattttc acttcatccg tcgcaagctt ctgcaatgcg
       61 taagccaaag cttcagctga cccctgcgca tcagccttaa tgatcagatt aagtatttta
      121 atatcatctt tctgattaaa tacctcctcc atcttagcga cttggcgctg atcgagtctc
      181 acatctctaa atttaccctg acggaaaagc gcaatctccc ttgcacgacg ctcatcctcc
      241 aacgcgagaa ccgtttcacc ggcaactgcc acatccgaca aaccttgaat ttctacagga
      301 atagacatac tggcactctc aatgggatta ccattttcat ccagcatggc tctaactttt
      361 ccaaatgctg caccagtcag caatacatca ccgcgcttta acgttcccga ctgtattaag
      421 attgtagcta ctggcccgcg tcctttatcc agccgtgatt cgatgacaac tccttttgca
      481 ggcgctgctt tgacagcttt aagttcaaga acttccgctt gcaataaaat actttctagt
      541 aattcatcaa ttcccagacc tgtttttgca gagacgccga caaaaatgac ctctccaccc
      601 caatcctcag caaccacacc gtgcgtgact aactcctgtt taatacgctc aaaattggct
      661 tctggtttat ccatcttatt tacagcaaca actatcggta ttttcgctgc cttagcatgg
      721 tgaatagcct caatcgtttg tggcataact ccatcatctg cagcaaccac taacacaacg
      781 atgtctgtaa tcttagcgcc acgcgccctc atcgccgtga aagcttcatg tcctggcgta
      841 tccagaaacg taatcattcc atgcgctgtc tcaacatgat aagccccaat gtgctgggta
      901 attcctcccg cctctcctcc tgcaacacgc gttcttcgaa tataatccag caaggatgtt
      961 ttcccatgat caacgtgacc catcactgtc acgacagggg cacgcggctc agctatagcg
     1021 tctgactgag aagctttctc ttcttccaaa taagattcag gattatcagg tgccgcatat
     1081 ttcgcaatat gacctaattc ctcaaccacg atcattgccg tgtcttgatc aagcatttga
     1141 ttgatcgtta ccataatgcc cattttcatt aaaacccgga ttacttcagc cgctttaact
     1201 gacatttttt gagcaagcgc agcaactgaa atcgtttcag ggaccattac ttcatattta
     1261 ataggctctg tcggctcagt aaatcgatgt aactcagcgc tctcatttga gcttgtctta
     1321 gcatttttat tggttttacg aatacgccaa ccatcagctt ggtttaagtc atgttttttt
     1381 atttcacgac gcttacccgt ttcttcactt agcagattct gctgtttgac ttgctttttc
     1441 tttttctccg gcttttcttc atgtttaacc gtaatagtcg atttaggctg ttgcgccgtc
     1501 ggttgagacg ccagctctgc tgtgtttgtc actttctcca caggtacttc ttgcttaggt
     1561 tcttccttta taacctcact aacttttggc tgtatcttct tctcagcttt ttttctgaat
     1621 tcaatttctt ggagttcaat caactttgca tggcgtctcg cttcttcctc acgctgcgct
     1681 agctcatcag cactaataat cggttttctt acagaagagg tctccggtac actaacagaa
     1741 acttctatcg gagcaatcgc tgctttggtt tctttatctt cagattgctc aactgtttct
     1801 tctacaggct ttgtataaac acgcgtttta cgaactttaa cctcaatcgt ccgctgctta
     1861 cctgccacat cagatttttt tatttcggtt gttttcttac gggttaatgc aattttcttc
     1921 tttgttgttt ctgccgcacc atgagactta cgaagatact cgagcaactg ctttttatcg
     1981 ccttctgtaa taatttcttc accagattgc ttactaacac cagcagtttg aagttgctct
     2041 agaagcgcat caggtagcat acctaactca ttggcaaatt gttcaaccgt tatttgagac
     2101 atcaaaaacc cttcacttac tccaaattta taaattgcag acttattccc gcattagttg
     2161 aggctctcat tgacggaaat gatagcaacc tcatacttat tcaataaaag ttacacaaac
     2221 caaggctctc gagccttaat aataatctga ttcgctcgct caatatcgat accagtcatc
     2281 tcaaccaaat catctgctgc taaatccgct agttcttctt gtgtattaat accttttgca
     2341 gcaagttccc gaaccagttg aatatccatt ccttctaaag cgagcaagtc ctcggaaacg
     2401 cgttcaattc tctcttcagt cgcaattgca gcggtaagca atgcattgct agctcgatga
     2461 cgtaattcag taatcgtctc ttcatcaagc gcttcaattt caagcatttc ttcgagtgga
     2521 acataggcaa tttcttctaa tgtagaaaac ccttcttcta cgaggatagc cgctacttct
     2581 tcatcaacat ccaatttatc gatgaacagt ttactgatga ctgaagtctc ttcttcattt
     2641 cgtttctgag attcctcctc tgtcatgata ttcaataccc aacccgtcaa ctcagaagca
     2701 aggcgtacat tttgaccacc tcttccaata gcttgcgcta aattttcatc attaacaacg
     2761 atatccatcg catgcttatc ttcatcaaca acgatactac tgatttctgc tggagcgagg
     2821 gcattaacca cataagtcgc aggatcctct gaccatagaa tgatatcaat ccgttcacct
     2881 gtcaattccg ttataaccga ctggactctc gagccacgca ttccgacaca agtaccaatg
     2941 ggatcaattc gtggatcgtt tgatttcaca gcaattttgg cacgtgagcc cggatctctc
     3001 gcagcagctt tgatttccag taatttttcc tctatctcag gcacttccag ttgaaatagc
     3061 ttaatcaaaa attcaggaga aattcgagat atgactaagc tcggcccacg cgaagtccga
     3121 tcgactcttt gaagataacc gcgaactcga tcccccaccc tcaaattttc tttaggaatc
     3181 atttgatctc gaggcaaaat cgcttcaatt ctacccgatt ctacaatggc gttaccacgc
     3241 tccatgcgct tgatcgtacc cgaaatcaaa tattctttac gatcaagaaa atcatttaga
     3301 ttttgctccc gttcagcgtc acgaattttt tgaaaaatga cctgctttgc agcttgtgca
     3361 ccaattcgat caaactctaa tgtttctata ggctcttcaa taaaatcacc taatttgatc
     3421 tcagcattaa gctttaaagc ctcgctcaat ataacctgcc gaaaaggatt ctctaatgca
     3481 tcatccggta caacctgctt actctgaaag catcggtaat tccctgtatc tctttctatc
     3541 ctaacctgta cttcaatatc ttcacggaaa tgttttttag tggccgacgc caatgccatc
     3601 tccaaagcag taaaaacaac cgctttactg acattctttt catgcgccaa tgcatctact
     3661 aatagtaaaa tctcgcggct catagtcctc caaccaaata tttattacgc ctatagttct
     3721 ggaaccaacc gtgctttatc taaattattt aactcgatat gtagaatttt cccttctatc
     3781 tccaaaccta atttaccgtc actcacttcc cgcagaatcc caacaaaatt tctctggccc
     3841 tgcaaagcaa tatgcgattt aaccttaact aatcttccta aaaaacgcac aaaatcacgc
     3901 tctttcctga gaggcctatc caaacctggt gaagaaactt caagtcgccc ataatcaatt
     3961 tgttctacca ccagcaaccg actcaaatga ttactgatcg ccacacaatc atcaatggag
     4021 atcccacctt ccttatctac aaatacacgc aataacttgc cagatggtga aaattccaca
     4081 tccaccaatt catagcccat attgttcagt gttgaatcca ataattcgta taaagccata
     4141 gcgcaaccac aaataaaaaa tgggctaata agcccacccc tgatatacgc tctattataa
     4201 caacaatcag atatcaacaa aatagaaatt tattttctag caatgtttcc aatatgttag
     4261 cgcccattat ccaatcaaaa aaacaacatt tgcattaata ttagcctgag caatctccaa
     4321 atctgctgct aaatttccat tgattatttc tagcctaaag ttttcagtat ttaatttgcc
     4381 gcaatcgcat attgactttc caactcattc atctgcgatt cagaatgata atttgccatt
     4441 gctggaacaa tcgtatcact aacaatcctg ccagcaacca actgaatcac tcgatctgcg
     4501 cttgcaatcg tttcgggacg gtgtgcgacg ataattcgcg ttaacttaag cgtgtgaatc
     4561 gcttggttaa cattctgctc acaatgaata tcgagatgac tcgttgcttc atccaaaaaa
     4621 agaatggccg gttgtcgata caatgcacgt gcgagcaata cccgctgttt ctgtccacca
     4681 gacaaaacag cccccatatc acccactaac gtattgaatt gcatcggcat tgccataata
     4741 tcatcatgaa tggcagccag acgagcacta gccataactc gctcaaaatc aggggttgca
     4801 tcaaaaaagc aaatattctc ggcaatcgag cccgcaaaaa gctgatcatc ctgcatcaca
     4861 ctggccacag cctgacggta tcgcgccaga ctcatccggc tcaaaggcac accatcgatt
     4921 tcgatctgtc cgcctgtggg ttgtaacaaa cctagcatga gtttaaccaa ggtcgtttta
     4981 ccacagcccg atggcccaac aattgccact gactcacctg cttgaacgct gaactgaata
     5041 tcttgcaaaa ccagaggatc cgtttcacta tgccggaaag ttagatgttt aacctcaatt
     5101 cgacctgtta ggggggtagc agaaatcgga tgtagttcgt taacttgttc aatcggtgtc
     5161 agcgccacgt ctcccactcg ttcggtatga agacttaaca tacgcagctc tagtcctttt
     5221 tcgattaatg ctgaaattct ttgcacaaac tgagttttat acgcaataaa cgcaaatagc
     5281 attcctatcg aaaatccagt tcctgttgct gtgtcgagta tcaacatcgc gccgatccat
     5341 atcgtaataa tattttccaa gccgaataaa agaccattta agccctggta taaaatattg
     5401 aatcgctgag tacgaatgct cgcgttgaac tgatcgatca ccaaattttg ccaattcgca
     5461 gcgcgtgatg cctcataagc aaacagctta atactttgta tcccgcgtac ggtttccaga
     5521 aaatggcttt gctgctttgc cccccttacg atatggtctt ctgtcgccaa acgaagtggc
     5581 cgatatagca ctaaacgcag taacccatat aatcctgctg cggccattgc aatcaacgcg
     5641 agcttgacac tatagagcag catcatacct aacgtaatca acaccatcac accatcgatt
     5701 aatgcttcga gaaaacccgt cgtcaaggtt cgctgaataa cattcaacga gtcgaaacga
     5761 gacacaatat cgccaatatg acgcttattg aaccactgca ttggcaagcg gatcaaatga
     5821 tggaataagt ttgtcatcat ttgcagattc agtgttgtac ttaacaccat caacacccaa
     5881 gatctcaatg cagtcacact cacctgaact accgctaaca aaagaaatcc cacacccaag
     5941 atggtcacaa gatcatgatc ataggcgacc aacgcatggt caacgaccca ctgcatataa
     6001 aaaggtgcca aaatggcaaa aacttggagg atcaatgcga gaatcaagat ttggctaatc
     6061 gcaccagtta aaccccgcaa tcgaccaatc aaggaccgca acttgactcg ctgaatctca
     6121 ttgcgcgtaa caaaatcact agtaggggtt aattcaagtg caatacccgt aaaatgcttt
     6181 gagaattcag ccaacgtcaa ttcacactca cccgaggcag gatcatgcag cacgacccgg
     6241 tcacgttgca cgcgtgtcaa gaccacaaaa tgattaaaat tccaatgtaa aatacaaggt
     6301 agtttcaact cagctaactg atccagatcc agacgtaagg gtcgtgaagc cagcttcagt
     6361 tgctcagcaa tttgaatcaa atccgcgagc gtagagccgc gcagcgaggc agcatgctgt
     6421 gcccgcaatc cgttcagatt gatttgatgc ccatgaaaac tcgcaatcat tgctagacag
     6481 gccaatccac actccgctgc ctcagtttgt agaataaccg gtgttcggtt aaataaagag
     6541 aaatgcaaca tgatcaaata cgtcctgtaa tcgacaataa aggatcaaac acccattcaa
     6601 tcaaacgtcg tcggtccaga tgaatatcag cgtctaatat catttctggt tgcagtggaa
     6661 gcaactcacc gtaggctatt atttgctgtt gtggtaggcg caccgtcgca cgataaactg
     6721 attcctggat ggatactggt aaattagcct cattgggttg aatcacagca cgactaacct
     6781 cgataatctc ccccaaatga tgtccaaagc gttgataagg aaatgcctga taacgcaaag
     6841 aaaccatttg ccctggtttg atgaatccaa ctgaatgcgt aggaacgagc aattgggcct
     6901 gcaactgcgc accggcaggc aaaatagaca gcaggggcaa attaggttga gccatttgcc
     6961 ccatttcggc taaaagtgta gtcactatcc cttcagtcgg cgcagtcagc acgatactgc
     7021 gacgcagatc tgtttcggta agttgttgct ccaattcgga aatctgccgt tcgatcgctg
     7081 ctcggttatt cactttcttt agaccagaag ctgacaactc ggatcgagcc gtattcaatt
     7141 cacgattgag gctagcaata gtccgtttga tctgaacaag ctgattacgc tgatcaagca
     7201 atgcttcttg tttctcctgc agagcaactt ccgacatgaa tcgctctgcc accaattgct
     7261 gatagcgtac caccatacgc tctgcactgg ctacacggct cgattgcaat gctaattgcg
     7321 cctgtgtttc acgaatttca ttctcaagtc cacgaatgcg tgcattaatg ctggtaccgg
     7381 ttagtgcatc gatttcgctc tgtttacctt gctcaaaacg caagctcacc tgccgctgtt
     7441 ttaactcggt gagcatcgct gcttgagcct cacgcgcgtt acttgttgtt cgttcactcg
     7501 aaatcgtcaa taaaggatcg ccttgtttta caacttgccc ttcccttaca tgcttctcct
     7561 gtatggtacc taattgcggt gaataaatac gtatcatacc tgtggtgggc gataaatagc
     7621 ctaccacatg ggtcttacgc gtatactcac cccaataagc aaaagccagc aacccaaggg
     7681 taaaaataag cgccacccag acaaacaaac gaatcgttaa tgatcgcgca atcagtactt
     7741 caccttgtcg agtatcttgc tgatgggcga ttgcctcaga acgaaaaagc ggtcgtgcca
     7801 tgcacaaact cctggaaatg aaatagtcac tgcattcaaa taatggtgca atcgcgagta
     7861 tattgcgatt gcaccgaact actattcatc aaaaccacat atcgccatac atttctttat
     7921 atgcagcaag cttgccgaca ttaaccccct catccatttt catcatcccg cctgcgatca
     7981 aacttaattc atctgcggtc agcgtatgga cttcaatctc atctaaagaa actgattcat
     8041 taaaatcaaa ttcattcatg attattctcc taagcaattt aatttaatta acttcattca
     8101 aagttgacta agctttctct tctaagttaa ccctgtaagc gttaacttac tgcttctaag
     8161 caaaattgac tgtgcagaaa ctcacaccac cataaatttt ctaatttgat cttgtggatt
     8221 aaaaagagca gattctgccc gtttacctat cagactgttc caattcaaaa ataaaggtga
     8281 ggaagtattc gcaatttctt catacacttc acgacacagt gcatgaaaag ctggcaagcc
     8341 atattctcgc caatcttcca ataaggtact cagcggaaat gcttttaaat tacccactga
     8401 gaaattccgc gagaacccat gctgaatcgg gctgactgtt ccatccgcct caatgaccag
     8461 tggtgaaatg agctcaccaa acggctttgt ctctagatta gcgcaatcgc ctgcaaacac
     8521 gagttctggg tgctgtttaa tcacttcgct aaaagcaaga tcaacttgga ttttgatatc
     8581 gctgtcgatt ttttgctgtg cgaggatcca ggcataagcg ctttcggtcg catccggtga
     8641 gttccctttt aatcgagtcg ctgcattgcc tacttcttcg aggggatgaa tttgcagtaa
     8701 ttttgcccct tcacccatcg cgaattcacg aacccaatta agctcatcca agttatattg
     8761 cgttagcgta aaaatgaaac caaacgtcaa accctctgcg cgtaataaag gtaagcggga
     8821 tctcatcgta tcaaacgcga tctgagaacc acggatccgg ttatgagatg agggaacccc
     8881 atcgatactg atggcaagca catcaacaac ctcacgcagt tgtgcaattt tctgctcggt
     8941 taacagcatg ccattcgttg taacggctgt gcgcattcct aattggtgag catgctcaag
     9001 caactccact aacggcttgt aaagcattgg ttcaccaccc gaaaggctaa cgtaattaaa
     9061 accttccgct gcggcatcag aaatcgcatc acataacaac ttaagcgtta aatgctcctt
     9121 ctcgtgcgga gaggaagagg aatagcaatg gagacaactc aaattacatt gccgagaagg
     9181 gtgaatctgt aaaatgcgag ataatccggt tggccccatc gctagcctcc ttcttagaat
     9241 tgttgctgat ttatattgaa ctcattcagc tgtaaaggct gaaacttaat aaaatgagga
     9301 tcaaacggta tcccattgat caaccagtat ggagaaatct gtccagacca tgaaaatccc
     9361 cccgcaactt gggcaatctg ctcgggcgtt aaacttgcca tggtttgaca tgttgctttc
     9421 aatttttcag aaccacgcat ctcttgtgac atgattgaat ctccttaaaa aaattaataa
     9481 attgaataaa tcgattgatg atccattgcg ggctgaattc caagatatct ccgccaagac
     9541 tacaaaccat cacgccatct tgattaccaa ctcgcattgc attcaatctg gttcaacaat
     9601 aattccaaat tcgaggcaaa acagtgagaa aaggcacaaa agaacaaaac taagccatta
     9661 attcgttttt aaggaaggga atgcgcctaa cccaaagcgc aaataacagg acaaccctgc
     9721 gataccagaa agaacatctg tttaaacgaa gcaaacattg ggagcaattg agtttatcga
     9781 taaacccgat tgctcccttt gctgattcaa ctcagcttat tgctaataac caaccgtaaa
     9841 gccttgcagt tcagcaggtg gtaatggttg tggattcaag c
//
LOCUS       DAOQLJ010000083         9813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2699_length_9813_cov_5.351199, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000083 DAOQLJ010000000
VERSION     DAOQLJ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9813
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2699_length_9813_cov_5.351199"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /gene="eno"
                     /locus_tag="PK522_07030"
     CDS             complement(<1..301)
                     /gene="eno"
                     /locus_tag="PK522_07030"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNC41524.1"
                     /translation="MSAIVDVIAREILDSRGNPTIEADVLLESGALGRASVPSGASVG
                     TKEAIELRDEDEERYFGKGVTKAVDNINTEISETIMGLDASEQAFIDQALIDLDGS"
     gene            complement(364..2031)
                     /locus_tag="PK522_07035"
     CDS             complement(364..2031)
                     /locus_tag="PK522_07035"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNC41525.1"
                     /translation="MTKYVFVTGGVVSSLGKGIAAASLAALLETRGIKVTMLKLDPYI
                     NVDPGTMNPFQHGEVFVTDDGAETDLDLGHYERFISTKMAKRNNFTTGQIYESVIRKE
                     RRGDYLGGTVQVIPHITDEIKLFIRNGVNDAQVAIVEIGGTVGDIESLPFLEAIRQMA
                     VQHPRDDTCFIHLTLLPYIGSAGELKTKPTQHSVKELREIGIQPDVLLCRSDRMLPQE
                     ERRKIALFTNVKEEAVISAIDVDCIYKIPALLHEQMLDEIICHKLNILAKPANLSVWK
                     NLVYALEHPTKTVNIALVGKYVDLTESYKSLSEALIHAGIHTHCKINICYLDSELIEK
                     EGTDCLSDKDAILVPGGFGKRGVEGKILAIHYARTHKIPYLGICLGMQLAVIEYARNE
                     LHLENAHSTEFNPNTPYPVLGLITEWRTREGQIEKRSEATDLGGTMRLGGQLCFLNED
                     SLAKKIYGSDKITERHRHRYEVNASFIPSLEKAGLKISGVSAEEKLCEMVELSQDEHP
                     WFVACQFHPEFTSTPQKAHPLFKAYINAAISYANQRTDNSLEQKEIA"
     gene            complement(2121..3884)
                     /gene="sdhA"
                     /locus_tag="PK522_07040"
     CDS             complement(2121..3884)
                     /gene="sdhA"
                     /locus_tag="PK522_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HNC41526.1"
                     /translation="MTIRKRKFDVVIVGAGGAGMRAALQLSEAGLTVAVLSKVFPTRS
                     HTVAAQGGIAASLGNVTEDNWHWHMYDTVKGSDYLGDQDAIEFMCRQAPEVVYELEHF
                     GMPFDRLDNGKIYQRPFGGQSQNYGGAQATRSCAVADRTGHAMLHTLYQRNLYANTQF
                     FVEWMGLDLLRDQQGDVLGVTALEMETGEVMILQAKVTLLATGGAGRIFQASTNAFIN
                     TGDGLGMAARAGIPLEDMEFWQFHPTGVHGVGVLISEAVRGEGGYLLNKNGERFMERY
                     APNAKDLASRDVVSRALTTEIKEGRGCGKDGDYLLLKLDHLGPEIIKSRLPGIREIAI
                     KFAHADPIEEPIPVVPTAHYMMGGIPTNFYGQVVAPYKTGPEEVVSGLYAVGECACVS
                     VHGANRLGTNSLLDIVVFGRAAGNKIIEELKHNPHHKSLPEDIADISLARLNRLDTQS
                     NGEKVADVGDALRRTMQTYCGVFRFPDMLKEGVSKINEVAQRVARTEINDKSKIFNTA
                     RIEALELDNLIEAAIATMVSAEARHESRGAHTRDDFPERNDEKWLKHTLYFKEGQRLD
                     YKPVRLKPLTVDSFPPKARVY"
     gene            complement(3886..4236)
                     /gene="sdhD"
                     /locus_tag="PK522_07045"
     CDS             complement(3886..4236)
                     /gene="sdhD"
                     /locus_tag="PK522_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HNC41527.1"
                     /translation="MVARTKRVLTGAHYGLIDWLVQRVTAVLMVLYLLLLAWVFTDQA
                     SDYAGLKSIFANQWMRIFSLLVFIGLCWHAWVGVRNVLMDYVKITSIRLSVQVLVITA
                     LLFYLIWFVDVLWR"
     gene            complement(4230..4619)
                     /gene="sdhC"
                     /locus_tag="PK522_07050"
     CDS             complement(4230..4619)
                     /gene="sdhC"
                     /locus_tag="PK522_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HNC41528.1"
                     /translation="METKFQSRRPKYLNLVKIKQPLPAIVSILHRISGVLLFFPGLPL
                     LLYCLQQVLESPESYAQFQSVIANPIIKWGFIFFLWALVHHFCAGIRHLALDLHLGST
                     LEGARLGSKLVVAFSIGITVLLGVLLW"
     gene            5110..5952
                     /locus_tag="PK522_07055"
     CDS             5110..5952
                     /locus_tag="PK522_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41529.1"
                     /translation="MRIDQIIIKLIVLPILLVVHYAHASYLDQTTPNGQVRIHYSSTT
                     PASCDAILLGVGTSMSISSYDKLSEELIKYGYVTVIMDHAPGNMVKTDPQKYATLAND
                     VKTNLNSWLSGIGCNTVTHWLMGGHSAGGQAAQNAISNGLVQADAIFSIDPYNANNAG
                     NVNLPAMYWGFDVTTCFVDKNAAAKAAYLHSQNLRAFYRVAKQYSWGPCGYSPKYFHC
                     SFCDEHCPACTNCVLTPQSFFVDVANSVNKFITALFYGNWSKSALTISATTPLTLFVD
                     SELP"
     gene            complement(5975..6775)
                     /locus_tag="PK522_07060"
     CDS             complement(5975..6775)
                     /locus_tag="PK522_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41530.1"
                     /translation="MFQNELLSSYPVNRIILVGASNLTLSLRMIIQHIQGSVGGPSEI
                     LVAAGHGRSYGLSSQVMIRELPGIVRCGLWEQLSTRVIIPSYALLTDIGNDIPYGVCP
                     EQILQWVTWCVDELTKASAQIVITNLPIESIEALSNRRFQFFRHLFFPSCTLSRIEVV
                     SRAKVVHQGIATLAQQKQLILCEQSRQWMGADGFHVAFMKREQVFRQLCNHFVGTDED
                     LSSVPKQSELRLSWQQRPRFALQRVWGKEKCCQQPSGLLSNQATVSLY"
     gene            6930..7793
                     /locus_tag="PK522_07065"
     CDS             6930..7793
                     /locus_tag="PK522_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNC41531.1"
                     /translation="MPAPNKIKQSLREIDSLPAMPVIAQKMLALPLDTTEGESQLLKL
                     ITQDPQISARIVGLSNSSFFSMPSTITSVSDAAMLLGLTQVKSIAIGMATITVLAKLP
                     EGKLKSMDLWAHSMAIATTMRTIARHMPANLRPIDDQIFLAGLLHDIGYSTLSYLDTE
                     TSNRLHEQLNNTANADLLAIEQEIVGIHHGEIGAQLGIHWGLPEELIAVMRYHHTPDH
                     PDATLGLPIIRLVNTAEKLLSNFWINEHTAQEITKQEWSNLGISENKIDSISEEVKTL
                     AENASQLVSAA"
     gene            complement(7817..8869)
                     /locus_tag="PK522_07070"
     CDS             complement(7817..8869)
                     /locus_tag="PK522_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC41532.1"
                     /translation="MRFQKRKIVLAIGCISALTGCASYFSGKEFSAYEVRPVTNIQQA
                     ESSAENFYHIGEYYQRKKQWLNALESYQKALSLQTEYVEAHNGLGIVYATLGQYELAI
                     DHFKKAITLDASKSYLHNNLGYAYYLQGVYHEAELSFQAAISNDPRNGKAKKNLTLVH
                     KKMGVSEKLARTLKATSNSETMSTKYPAQLTHENHLSQQLVQVAPNVYEFRSQRETQN
                     KVENKLVSIVMHDEQETQLQKNIKSLAQGIEVSNGNGVTGMARNVATQLNNAGLGQAR
                     LTNHQTFKQVQTEIYHRVSAHALAEKITQLLPGQIKLIESDDLREDINLKILLGQDIA
                     NFKALSHNSHQVMKVM"
     gene            complement(8872..9372)
                     /locus_tag="PK522_07075"
     CDS             complement(8872..9372)
                     /locus_tag="PK522_07075"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC41533.1"
                     /translation="MLHCLRQLLILAGCFLLLVGCATQSTNSLLTQNDVPSVKKQMGP
                     DLVKLYLQGKKYQENANYSEAISAYEAILAKDPDFVEAHNGLGVIYSAQEKFELALQH
                     LQTAVKLAPLATHLHNNLGYTYFLLGQDEKAIKAFEQSLYLDPENERAQFNLSLAHQK
                     ISKSGE"
     gene            complement(9384..>9813)
                     /locus_tag="PK522_07080"
     CDS             complement(9384..>9813)
                     /locus_tag="PK522_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNC41534.1"
                     /translation="VCVEAGLGLDAALAKVGEEMELRSPILAEEIHLVGLELRAGASR
                     EKALRNLALRTGVEEIEALVAMLVQADRFGTSTADSLRVYSDTLRTKRRLRAEEAAAK
                     IALKLLFPLIFFIFPALMVVLMGPAFISIYRNLLPALAGQ"
BASE COUNT         2844 a         2336 c         1836 g         2797 t
ORIGIN      
        1 cactcccatc taaatcaatc agtgcctgat caatgaatgc ttgctctgac gcatctaacc
       61 ccatgatggt ttcggaaatc tcggtattga tattatctac tgcttttgtc acgcctttac
      121 caaagtagcg ttcctcatct tcatcacgca attcaatcgc ttctttggtt ccaaccgaag
      181 cccccgacgg aacggatgcg cgccccagcg cacctgactc taacaaaaca tctgcttcta
      241 tggttggatt gccacgagaa tctaaaattt ctcgcgcaat aacatctact atcgcactca
      301 tgctactttt ttccttgtaa taatcgactt aatgtactaa tgcgcttctc tcttgctttg
      361 tttctaagca atttctttct gttctaaact gttatctgta cgctgatttg cgtagctaat
      421 cgcggcattg atatatgctt tgaataaggg gtgtgctttt tggggagtag atgtaaattc
      481 tggatgaaac tgacaagcaa caaaccaagg gtgctcgtct tgggataatt caaccatttc
      541 gcataacttt tcttcagcag atacgccact tatttttaga cccgcttttt ccaagctggg
      601 aataaaactc gcattcactt cataccgatg gcggtgacgc tcggtaattt tatcagatcc
      661 atagatcttt ttggccaatg aatcttcgtt gagaaaacac aattgccctc ccaagcgcat
      721 cgtcccaccg agatcagtag cttccgaccg cttctctatc tgaccttcgc gagtccgcca
      781 ctctgtaatg agaccaagaa ccggataagg ggtattggga ttaaattctg tactatgagc
      841 attctctaaa tgcagctcat tgcgtgcata ttcgatgact gcgagttgca ttcccaaaca
      901 aataccaaga taaggtatct tatgggtacg tgcataatga atggctaaaa tttttccttc
      961 aacccctcgt tttccaaatc cacctggaac taaaattgca tccttatcgc ttaaacagtc
     1021 cgtaccttct ttttcaatga gctcagaatc caagtagcag atattgattt tacaatgcgt
     1081 atggatgccg gcatgaatca atgcttctga caaagattta tacgactcgg tcaggtctac
     1141 atatttgcca accaatgcga tattgaccgt tttagttgga tgttctagcg cataaaccag
     1201 atttttccaa acggaaagat tagctggctt tgcgaggata ttgagcttgt ggcagataat
     1261 ctcatcgagc atttgctcat gaagtaacgc tgggatctta taaatacaat cgacatcgat
     1321 cgcagagatc acggcctctt ctttcacatt ggtaaatagt gcaattttgc gacgctcttc
     1381 ttggggcagc atgcggtcgg aacgacaaag caacacatcc ggctggatcc cgatttcacg
     1441 caactcctta accgaatgct gggttggttt tgttttcaat tcacccgcag aaccaatata
     1501 aggtaaaagc gtcaaatgga tgaaacaagt atcgtcacgg ggatgttgta ctgccatctg
     1561 ccggatcgct tccaagaatg gcaatgattc aatatcccct actgtacccc caatctcaac
     1621 aatagccacc tgagcatcat tcacgccatt gcgaataaac aatttgattt catcagtaat
     1681 atgggggata acttgaactg tccccccgag ataatcgcct cttctctctt tgcgaataac
     1741 gctttcgtag atttgacccg tcgtaaaatt attgcgcttg gccattttgg tactgatgaa
     1801 gcgctcataa tgacctagat ccaaatcggt ttccgcaccg tcatcggtga caaacacctc
     1861 tccatgttgg aatggattca tggtacccgg atcgacattg atataagggt caagcttcaa
     1921 catggtcact ttgataccac gtgtttcaag caatgcagcc aatgatgcag ccgcaatccc
     1981 tttacctaat gaagaaacaa ctccgccagt tacaaagaca tacttagtca taatggaggg
     2041 caatgttaac ccaaataata aaccatggaa actatctatc gtttccatgt tattcaaaaa
     2101 tgaaacatct ctcgatgttt ctaataaact ctcgctttag gtggaaatga atcaactgtc
     2161 aatggtttca atcgaactgg cttataatcc aagcgctgac cttctttgaa atagagcgta
     2221 tgttttaacc atttttcatc attgcgctct gggaaatcgt cacgcgtatg cgcaccacgg
     2281 ctttcgtgcc gagcctcagc agaaaccatc gtggcaatcg ctgcttcgat taaattatct
     2341 aactccagcg cttcgattcg agcagtatta aaaattttgc ttttatcgtt aatctcagtg
     2401 cgtgcgacac gttgcgctac ttcattgatt ttgctaaccc cttctttcag catatccgga
     2461 aagcgaaata caccacaata ggtttgcatg gttctgcgaa gtgcatcacc cacatccgct
     2521 actttctcac cattactttg cgtatcgagg cgattcaatc gtgctaatga aatatcagcg
     2581 atatcctctg gtaacgattt atgatgggga ttatgtttaa gctcttcgat aattttgttc
     2641 ccggctgcgc gaccaaaaac tacgatatcg agtagcgagt tggtacctaa acgattggca
     2701 ccatgcacgg aaacacatgc acactcccct actgcatata atcctgatac cacttcttca
     2761 gggcccgttt tataaggtgc aacgacttgc ccataaaaat tggtgggaat tccacccatc
     2821 atgtagtgcg cagtcggcac aacgggaatc ggttcttcta tgggatcggc atgagcaaat
     2881 ttaatcgcaa tctctcggat accgggtaat cttgatttaa tgatctccgg acctagatgg
     2941 tcaagtttta gtaacaagta atcgccatcc ttgccacacc ctctgccctc tttgatttct
     3001 gtagtcaatg cacgcgatac cacatcacga ctcgctaaat ctttggcgtt cggtgcatag
     3061 cgctccataa agcgttcgcc atttttattg agcaaatacc caccttcacc gcgcactgct
     3121 tcactaatca gaacaccaac tccatgcacg cctgtcgggt gaaattgcca aaattccata
     3181 tcttccagcg gaatacctgc acgcgctgcc atgcctaatc catcgccagt attaatgaac
     3241 gcattggtac ttgcctgaaa gatacgccct gccccacccg tcgctaacaa agtaaccttg
     3301 gcttggagga tcatgacttc ccccgtttcc atttccagcg ccgttacacc aagtacatcc
     3361 ccctgttgat cacgtaatag atcaagaccc atccattcga caaaaaactg agtgttggca
     3421 tataaattac gctgataaag cgtgtgtagc attgcatgac ccgtgcgatc agccactgcg
     3481 caagatcttg ttgcttgtgc tcctccataa ttttgcgatt gaccgccaaa agggcgctgg
     3541 taaatcttcc cattatcgag acgatcgaat ggcataccaa aatgctctaa ctcataaacc
     3601 acttcaggcg cctgtcggca cataaattca atcgcatcct gatcgcccaa gtaatcagaa
     3661 cctttcacgg tatcgtacat atgccagtgc cagttatctt cggttacgtt acccaatgat
     3721 gctgcaatcc caccttgtgc tgctactgta tgagagcgcg tcggaaaaac cttagataat
     3781 actgcaaccg tcaaaccggc ttcagataat tgcaaggcag cacgcattcc agcaccccct
     3841 gctccaacaa tcacgacatc gaattttctt ttgcggatgg tcacattatc tccatagcac
     3901 atcaacaaac cagatgagat aaaacaacaa agcggtgatg actaaaacct gcacgctcaa
     3961 tcgaatactg gtaatcttca cataatccat caatacattc ctgacgccaa cccaagcgtg
     4021 ccagcataaa ccaataaaca ccagtagaga aaatatcctc atccattgat tagcaaaaat
     4081 cgattttagc ccagcataat cacttgcttg atcggtaaat acccaagcaa gcaacagtaa
     4141 atataaaacc attaatactg cggtaacgcg ctgaactaac caatcgatta acccataatg
     4201 tgcgccggtc agaacccgtt ttgtgcgcgc taccataata agactcccaa taaaacggtt
     4261 atcccaatac taaaagctac aactaactta ctccctaagc gtgcaccttc caatgtgctc
     4321 cccaaatgca aatcaagtgc caagtggcga attcctgcac aaaaatgatg aaccagtgcc
     4381 caaaggaaga aaatgaaacc ccatttgatg atgggattgg cgatcacgga ttgaaactga
     4441 gcgtaacttt caggtgattc taatacttgc tgcaggcaat aaagcaataa gggtaaacca
     4501 ggaaagaaca gtaatacacc gctgatccga tgcagtattg aaacgatagc agggagtggt
     4561 tgcttgattt taaccagatt caaatactta ggtcgtctgc tttgaaattt agtctccata
     4621 ccttaaatca ctgacctact gaattccgat atcattaaaa aggttactgc tagtttaatc
     4681 ccttaaatca attcattcaa cgctttaatc tcgacttata gtaaacaagc aaacttttag
     4741 ctaaaccaag cctatcaatc acagtgcatc tacgcagaaa gatgattgaa accacacgat
     4801 cacacaagaa ttgaatcgat tcaacagcaa ggcggttgaa aaaaactgca gcgtggataa
     4861 gaggtagcgc gttttggcaa aggtaaacaa aaaaatgggc ggcaattata ggctattttt
     4921 tgtccattga agccacggta attaaaccaa aagatcgtta atttaacaaa atcatcttta
     4981 atagcagcat tctataattt cacaatgaaa aattagcacc acattgatta agcttttttc
     5041 accatcgcgc taaccgttag tcaaaataac cttgactatc tcggccattc atcaaggaga
     5101 atcattccaa tgcgaatcga tcaaatcatc attaaattaa ttgttctgcc aattctattg
     5161 gttgttcatt acgctcatgc gagctaccta gatcaaacaa cacccaacgg tcaagtacga
     5221 attcactatt catccacgac acctgcaagc tgtgatgcaa ttttactcgg cgtgggtaca
     5281 tcaatgagta tctctagcta tgacaaatta tcagaagaat tgatcaagta cggatacgtt
     5341 actgtaatca tggatcatgc acctggtaac atggtaaaaa ctgaccctca aaaatatgca
     5401 accctggcta acgacgtcaa aacgaatctc aatagctggt taagtggtat tggttgcaac
     5461 actgttactc attggctaat gggaggtcac tcagcgggtg gtcaggccgc tcagaatgca
     5521 atttcaaatg ggttggtgca agcggatgcc atatttagca tcgatcccta taatgcaaac
     5581 aatgccggca atgttaattt acctgcaatg tactggggat ttgatgttac tacctgcttt
     5641 gttgataaaa atgcagcagc aaaagccgcg tatctgcaca gtcagaattt acgcgcattt
     5701 tatcgtgtcg cgaaacaata cagttggggg ccttgtggct actctcccaa atattttcat
     5761 tgcagtttct gtgatgaaca ctgccccgcc tgcacaaatt gtgtcctaac gcctcagtca
     5821 ttctttgttg acgtagcgaa tagtgtcaat aaattcatta ctgcactgtt ttatggaaat
     5881 tggtcaaaaa gtgcattgac catcagtgca acaacgccat taacactttt tgtcgacagt
     5941 gaattaccct gaacttgatc agtattgagc accattaata aagcgatacg gtcgcttgat
     6001 tcgataataa accgcttggt tgttggcagc atttttcttt accccaaacg cgttgcaatg
     6061 caaaacgagg tcgctgctgc caacttaatc gaagctctga ctgtttaggg actgatgata
     6121 aatcttcatc agtccctaca aaatgattac atagctgtct aaaaacctgc tctcgcttca
     6181 taaatgcgac atgaaaacca tcggctccca tccattggcg agattgctca cagaggataa
     6241 gttgtttctg ttgtgcgagt gtagcgatac cttgatgtac cacctttgct cggctaacga
     6301 cttcgatcct cgataaggta catgacggaa aaaataaatg cctaaaaaat tggaaacgcc
     6361 tatttgatag cgcttcaata gattctatcg gtaagttggt aattacgatt tgtgcagatg
     6421 cctttgttaa ctcatcaaca caccaggtaa cccattgtag aatttgctca ggacacacgc
     6481 catagggaat atcgttgcca atatctgtca ataaagcata acttggaata attacacgcg
     6541 tgctgagttg ttcccataaa ccacaccgaa caataccggg taactcccga atcatcactt
     6601 ggctactcaa accataggag cgcccatgcc ctgccgcaac taatatttca cttggaccac
     6661 cgacactccc ttgaatatgc tgaataatca tacgaagcga taacgtcaga ttgctcgcac
     6721 cgactaaaat gattctattt actggataac tcgagagtag ttcgttctga aacattttca
     6781 ttgctttata tcaaaatagc aaaagattat ggcctagttc tacaattcta gcgaaaacga
     6841 ttagcgtaga attcataaag ttaccctaaa ttcagtaacc tcatctgaat tagatccttg
     6901 attaaagcgc tctttggagg aaatcaacca tgccagcccc aaataaaatt aaacaatccc
     6961 tgagggaaat tgattcactt cccgctatgc cagttattgc tcaaaaaatg ctggcactcc
     7021 cgttagatac cactgagggt gaatctcaat tacttaaact cattacacaa gacccccaaa
     7081 tttctgctcg aatcgtcggc ctctctaatt catccttttt tagtatgcca agcacgatta
     7141 cctcggttag cgatgccgca atgttacttg ggttgaccca agtcaaatcg attgcaatcg
     7201 gtatggctac gattacagta ttggccaagt tacctgaagg aaaattgaaa tcgatggatc
     7261 tatgggctca tagcatggct atcgcgacaa ccatgcgtac tattgcccgg catatgccag
     7321 caaatcttcg tcccattgat gatcagattt ttctagctgg tcttttgcat gatattggct
     7381 acagcacgct gagttatttg gacacagaaa ccagcaatcg cttgcacgag caattgaata
     7441 atacggctaa cgcagattta cttgcaatcg aacaggaaat agtcggtatt catcatgggg
     7501 aaatcggtgc tcaacttgga atacactggg gcttacctga agagctcatc gctgtgatgc
     7561 gctatcacca tacccccgat cacccagacg ctacactagg attaccaata atcaggcttg
     7621 ttaataccgc tgaaaaatta ctttctaatt tttggattaa tgaacatacg gctcaagaaa
     7681 ttactaagca agagtggagt aatcttggta tttctgaaaa caaaattgat agtatcagcg
     7741 aagaagtaaa gacacttgca gaaaatgcaa gtcaacttgt tagcgctgct taaagcagcg
     7801 cttaaaacta gaacgactac atcactttca ttacttgatg agagttatga cttaacgctt
     7861 taaaattagc gatatcttgg cctagcaaga tttttagatt gatatcttct cgcaaatcat
     7921 cactttcaat cagtttgatt tgccctggca ataactgtgt aattttttct gctagcgcgt
     7981 gagcgctgac acgatgatat atctctgtct gtacttgttt aaaggtttga tgattagtca
     8041 gtcgtgcttg gcctaatcca gcattgttca attgtgttgc cacattccgg gccatacctg
     8101 ttacaccatt accatttgaa acttcaatcc cttgtgcgag acttttaata tttttttgta
     8161 attgagtctc ctgctcatca tgcataacaa tacttactag cttgttttcc accttattct
     8221 gagtttctcg ctgtgatcta aactcgtaaa cgtttggtgc gacctgaact aattgttgtg
     8281 atagatgatt ttcgtgtgtt agttgtgctg gatactttgt tgacatcgtt tcactattac
     8341 tcgtcgcctt aagcgttcta gctaatttct cgcttactcc cattttttta tggactaacg
     8401 ttaaattctt tttagctttt ccattacggg gatcattgga tatcgcagct tgaaatgaaa
     8461 gctctgcttc atgatatacc ccttgcaagt aataagcgta gcctaaatta ttatgcaaat
     8521 agctcttgga agcatctaag gtgattgctt ttttgaagtg atcaattgct aattcatact
     8581 gacctagggt tgcataaaca atccccagcc cattatgggc ttctacatac tcagtttgca
     8641 acgataacgc tttctgataa gactcaagcg cattaagcca ttgttttttt cgttgataat
     8701 attcgccgat atgataaaaa ttctctgcgc tgctttctgc ctgttggata ttcgtaacgg
     8761 gcctcacttc atacgctgaa aattcctttc ctgagaaata cgaagcgcac ccggtcaatg
     8821 ccgatataca accaatcgct aagacgatct tacgtttctg gaatcgcatt gctattcccc
     8881 actttttgat attttttgat gcgccaggct taaattaaac tgcgctctct cattttctgg
     8941 gtcgagatac aaagattgct caaacgcttt tatcgctttc tcatcttgtc ccagcaaaaa
     9001 ataggtataa cccaaattgt tatgaagatg cgttgctagg ggcgccagct taaccgccgt
     9061 ttgcaaatgc tgtaacgcaa gctcaaactt ttcttgcgct gaataaatca cacccaatcc
     9121 attatgcgct tcaacaaaat cagggtcttt cgctaaaata gcctcataag ccgaaatggc
     9181 ttctgaatag tttgcatttt cttgatattt cttgccctgc aaatagagtt tcaccaaatc
     9241 gggccccatt tgctttttta cacttggcac atcattttgt gtcagcagac tgttagttga
     9301 ttgtgtcgca catcctacca ataataaaaa acagccggcc aaaattaaca attgtcttaa
     9361 gcaatgcagc atgatagaac tcctcattgt ccagccaacg ctggaagtaa gtttcgataa
     9421 atactgataa atgctggacc catcagcaca accatcaacg cggggaaaat aaagaaaata
     9481 agcgggaata ataatttcag tgcaattttt gcagccgctt cttctgcccg caaacggcgc
     9541 ttagttcgca atgtatcgga atagactctc agcgaatcag cggtactagt tccaaatcga
     9601 tcggcttgca ccaacatggc aactaatgct tcgatttcct cgacacccgt tcttaaagca
     9661 agattacgca gcgctttctc acgggaagca cctgcccgta attcaagacc aaccaaatga
     9721 atctcttccg caagaatcgg acttctcaat tccatctctt cacccacttt tgccaaagcg
     9781 gcatctaatc ctaatcctgc ttctacacaa acc
//
LOCUS       DAOQLJ010000084         9684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2739_length_9684_cov_5.142175, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000084 DAOQLJ010000000
VERSION     DAOQLJ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9684
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2739_length_9684_cov_5.142175"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..2061)
                     /locus_tag="PK522_07085"
     CDS             complement(112..2061)
                     /locus_tag="PK522_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC41535.1"
                     /translation="MLIQKASIIPATNQLSPAFFSATFTQYYSLKSLIPAYLICIWVL
                     LITFNTNAAPPAFINQNLTIQSSEAELLLEKPHPIKGTEKEEWITGRILVMPRAGLPN
                     KAFASILKEHQGKAQKIGQSDLYIVNLPEYTEEGVIAKLKHHPHIKYAELDYLLHSSF
                     VPNDQYYSKAWHHTKIGTPTAWDSTRGAGITIAILDTGVDNTHPDLTSNIVPGWNFYS
                     NNSNTADVNGHGTAVAGVAAASTNNTIGVASVAGSAKIMPVRVADANATASASTVAKA
                     LTWVADKGVRVANISYSGIPGNLTIQNAAQYMKNKNGLVIVAAGNSGTDLGYATTSTM
                     IPVSATDSNDIKTSWSSFGKYVAISAPGKDIWTTFRSNIYGIGWGTSMATPVTAGTVA
                     LMMAANPKLSSTQVESLLFSTAKDLGTAGRDSYYGYGRVNAAAAVQAAKAASTTTTLL
                     TAATDSEKPVASIINPANGATVSGLVSVDIEASDNVGVTRTELWVNNTNVAIDTLPPY
                     AFTWDSTGVPNSTANLVVRAYDEAGNSTTSDAISVTVSNQTTIAVNDTTPPQVSIVNP
                     ISGSVAGNVTITMNASDNSGSAGITLSLYIDNNLKAVGSGSTLSYNWNTKTKSVLEGG
                     HVIKAIAKDASGNTSTSTVNVTVVK"
     gene            2438..3178
                     /locus_tag="PK522_07090"
     CDS             2438..3178
                     /locus_tag="PK522_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNC41536.1"
                     /translation="MISYRYFLLPSVFILAACSSVENRQDPLETMNRAVFEFNEKFDY
                     YVFEPVAKGYKFITPDPLEMVIGNVFSNAGDVVNTANSVLQFKFADAAASATRVVINT
                     TFGMGGIVDLASDISRVSDIDISKRNEDFGQTLGFYGVGPGPYLVIPFWGPSSVRDAF
                     GVMVDSSLFHPFWSIYPDFDFLTVRLPTAVGWSIDTRAQMLDIDKTLEEASLDKYEFV
                     RDAYLQRRRSLVHDGNPPKEEQNGENGE"
     gene            complement(3290..4177)
                     /gene="htpX"
                     /locus_tag="PK522_07095"
     CDS             complement(3290..4177)
                     /gene="htpX"
                     /locus_tag="PK522_07095"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HNC41537.1"
                     /translation="MKRILLFIATNLAILIVLSITLRILGVDRVLDAQGGGLNFSALL
                     VFSAVLGFGGSLMSLAMSKWSAKHMTGAQVIEIPSNATEGWLVETVRKQAHAAGIGMP
                     EVAIYDSPDVNAFATGMSRNNALVAVSTGLLANMQQDEAEAVLAHEVSHIANGDMVTL
                     TLIQGVVNTFVIFLSRIIGHAVDRLIFKTEEEHGPAYMITSIIAQIVLGILASIIVMW
                     FSREREFRADAGGAKLAGRQKMIAALERLKQQYEPSHLPDKIATFGISGKQSQISRLF
                     MSHPPLDERIAALRGDTIA"
     gene            4397..5551
                     /locus_tag="PK522_07100"
     CDS             4397..5551
                     /locus_tag="PK522_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC41538.1"
                     /translation="MSQQPVVLIGSGLAGYGVARELRKLNSEIPITMLSADHGGFYSK
                     PMLSNAIATGKTPESILNSSAVQMANQLHITIRPDTRVSVIDSAQKAIVLENGEHLIY
                     GQLVLAMGADQIRLPLQGDGVADLLTVNDLDDYRHFRVALEGKKQIAILGAGLIGCEF
                     ANDLVGAGYEVHVIDISTQPLGRLLPSEGGRFLQEKLTAIGIKFHLKTITQRIDRMGD
                     RLSLSFDDGTQLEVDLILSAVGLRPRIQLAQAAGIEVNRGIVTDRYLQTRTPDIYALG
                     DCAEVEGKVLPFIMPISHASRALAVTLNGTPTLLRYPAMPVLVKTPACPTIVSPPDPD
                     KEGEWRVDKTEDGIKALFQDITGNLLGFALLGDATKEKNALTPLLPAVLD"
     gene            5710..6249
                     /locus_tag="PK522_07105"
     CDS             5710..6249
                     /locus_tag="PK522_07105"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HNC41539.1"
                     /translation="MSSRNSGWHPVPVSASSALRWWLLNQHSLTDLIKQRCNQFRVEP
                     TFQALARACHDELSVIDLHRNELALIREVYLYCGDRPVVFAHSVIARNHLQGVWRGLS
                     RLGNQSLGSLLFSNPLIKRQDRFSFRQLNDHHLLYHRACQRMSPNKPRRLWARRRSFS
                     LYGQPILVTEIFLPGILEL"
     gene            6252..7124
                     /gene="ubiA"
                     /locus_tag="PK522_07110"
     CDS             6252..7124
                     /gene="ubiA"
                     /locus_tag="PK522_07110"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNC41540.1"
                     /translation="MITKERLIAYARLIRLDKPIGILLLLWPTLWGIWLAAEGTPDAM
                     ILFIFIAGTILMRSAGCAINDFADRRIDPHVERTKNRPLAVGVITAKEAVVVASGLSF
                     CAFLLVLPLNTLTLQLSVCALFLAGTYPFTKRFFAMPQAYLGIAFSFGIPMAFAAQTG
                     ELPPIVWILLVANVLWVIAYDTEYAMVDRADDLKIGIKTSAITLGRYDIIGIMLCHSF
                     FLGIMTYVGWVLQLGVLYYLSLGVVVALVIYQYQLIKDRKPPGCFKAFLHNNWVGASI
                     FAGIALDSLIKIPF"
     gene            7146..8609
                     /gene="ubiD"
                     /locus_tag="PK522_07115"
     CDS             7146..8609
                     /gene="ubiD"
                     /locus_tag="PK522_07115"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HNC41541.1"
                     /translation="MKYKDLRDFLDQLALRGQLKRVTHPIDPYLEMTEICDRLLKQGG
                     PAVLFEQPKNHSIPVLGNLFGTPDRVALGMGQESMAALREVGKLLAYLKEPDPPKGLK
                     DAWEKLPVLKQVLNMAPKVVSSAPCQEIVWEGQAVDLSKLPIQTCWPGDVAPLITWGL
                     TITRGPHKSRQNLGIYRQQVIAPNKVIMRWLAHRGGALDYRDFCQVNPGMPYPVAVAL
                     GADPATILGAVTPVPDSLSEYQFAGLLRGAKTEVVQCLTHDLQVPASAEIVLEGFIYP
                     DETAIEGPYGDHTGYYNEQERFPVFTIERMTMRRNPIYHSTYTGKPPDEPAILGVALN
                     EVFVPLLQKQFTEITDFYLPPEGCSYRMAIVSMKKQYPGHAKRVLFGVWSFLRQFMYT
                     KFIIVTDEDIDIRDWKEVIWAITTRVDPARDTLIVENTPIDYLDFASPVSGLGGKMGL
                     DATNKWPGETVREWGRTITMDAAVKSRIDQLWDELPF"
     gene            8875..9300
                     /gene="ndk"
                     /locus_tag="PK522_07120"
     CDS             8875..9300
                     /gene="ndk"
                     /locus_tag="PK522_07120"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNC41542.1"
                     /translation="MAIERTLSIIKPDAVAKNVIGQIYTRFENAGLKIIAAKMMLLSR
                     TEAETFYAVHRQRPFFNDLVSFMTSGPVMVQVLEGEDAIKKNRELMGATDPKKADKGT
                     IRADFADSIDANAVHGSDAAETASVEIACFFPALSIFSR"
     gene            9334..>9684
                     /locus_tag="PK522_07125"
     CDS             9334..>9684
                     /locus_tag="PK522_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNC41543.1"
                     /translation="MNSVVNLLDYDSAGLVRFCEEIGEKPYRAKQLLRWIHQFGKSDF
                     AEMSDLAKLFREKLHSLATIRSPEVMSDYTAQDGTRKWLLATGQGNAVEMVYIPEVAR
                     NTLCISSQVGCALNC"
BASE COUNT         2529 a         1889 c         2339 g         2927 t
ORIGIN      
        1 ctctatcttg attgtaaaat tattcgacaa cctcaataac aagtatattc ggcttaggcc
       61 aagataaacg ccttaacgct atcttggcct agaaaaagga taaagcattt tttatttaac
      121 aactgtaaca ttgactgtgc ttgtcgaagt attgccagaa gcgtctttgg cgattgcttt
      181 gatgacatga ccgccttcta atacactctt tgttttagta ttccagttat agctcaatgt
      241 gctcccggat ccgacggctt ttaaattatt atcgatataa agcgataagg ttattcctgc
      301 tgagccacta ttatcagacg cattcattgt gatcgtaacg ttacctgcca ccgatcctga
      361 tatagggttg acgatgctaa cttgtggcgg cgttgtatca tttactgcaa ttgtcgtttg
      421 gtttgagaca gtgacactaa ttgcatctga ggttgtgctg ttaccggctt catcataagc
      481 gcgaacgaca agattagcag tgctgttagg aacacccgtt gagtcccacg taaatgcata
      541 aggcggtagc gtgtcaatcg caacattggt attgttcacc cataattcag tccgggtcac
      601 gccaacatta tcgcttgctt cgatatcgac tgatactaat cctgaaacgg ttgcaccatt
      661 ggcaggattt atgatgctgg ctactggttt ttcgctgtcc gttgctgcgg taagaagagt
      721 cgttgtggtt gacgctgctt ttgctgcttg tactgctgcc gcagcattca ctcgcccata
      781 accgtaataa ctatccctcc ctgccgtacc cagatctttg gctgttgaaa aaagtaaact
      841 ctcaacttga gtactactta gtttgggatt cgctgccatc atcaaagcaa ccgtgcccgc
      901 ggtgaccggt gtggccattg aagtacccca gccgattcca taaatattgc tcctaaacgt
      961 ggtccagatg tcctttccag gagctgatat ggctacatat tttccgaagc tagaccagct
     1021 tgttttaata tcattgctgt ccgttgctga aactggaatc atcgtcgatg tggttgcata
     1081 accaaggtct gtgccactat ttccagcggc aacgatgact aatccatttt tgtttttcat
     1141 gtattgcgct gcattttgaa tggtaaggtt accaggaatg cctgagtagc tgatatttgc
     1201 aacacgcaca cctttgtctg cgacccaggt tagcgctttt gctacagtgc tagcgcttgc
     1261 ggtggcattg gcatcggcaa cacgcactgg cataattttc gctgaacctg caactgatgc
     1321 tacaccaata gtgttgttag tggaggcggc tgctacgccc gcaactgcgg tgccatggcc
     1381 gttaacatca gcagtatttg agttgttgct atagaaattc cagccaggca ctatgttcga
     1441 tgtaagatct gggtgggtat tatcaacgcc tgtatcgaga attgcgatgg taatgccagc
     1501 gccacgggtg ctatcccaag cggttggggt cccaatttta gtgtgatgcc aggctttgga
     1561 ataatattga tcgttaggaa caaaactgga gtggagcagg tagtctagtt ctgcatattt
     1621 gatatgcgga tgatgtttta gcttggcaat aacgccttct tctgtgtatt ccggcagatt
     1681 gacgatatat aggtcacttt gaccaatttt ctgggccttg ccttgatgtt ctttcaggat
     1741 acttgcaaaa gctttgttag gtaatcctgc gcgaggcatg acaagaatgc gcccggttat
     1801 ccattcttct ttttcagtac ccttgattgg gtggggtttt tcaagtaata actctgcttc
     1861 agaagattga attgtgagat tctgattgat gaatgccggt ggcgctgcgt ttgtattaaa
     1921 agttattaac aatacccata tacaaatgag ataagcagga atgagcgact taaggctgta
     1981 gtattgcgta aatgtcgcag agaaaaaggc tggagataat tggtttgttg cggggataat
     2041 gctggctttt tgaataagca ttaggtgctt ctcctttttt tctatttgtt aattgaaaat
     2101 catactcatg cctgtcaagc aatgacttta gggggtagga taattgctaa ttgtataaat
     2161 cctttattag tcagcaataa gcatgccaaa tattaattca ttaataaaca gtgggttaga
     2221 attgaatctg ctggagttct gaagtcaagt gtcgctgctc tgagacgaag gggtgaatga
     2281 cttgatgtaa ctcactgtat taaatgatgg tttttacgtc gtcaatcaat gacagcgaat
     2341 aaaatagggg aaattcaaag ggtaaagata aagtaggttg tctcaactgc gtaattatgg
     2401 caagatgccc tatccatgta ataaaaagaa ttttaatatg atttcatatc gctatttttt
     2461 gttgccatcg gtgtttattt tggctgcttg ctcctcggtt gagaaccggc aggacccgct
     2521 tgaaacgatg aatcgtgctg ttttcgagtt taatgaaaaa tttgactatt atgtttttga
     2581 acctgtggcg aaggggtata aatttattac acctgatcca ttagaaatgg tcattggtaa
     2641 tgttttctcg aatgcgggtg atgtcgtgaa cactgctaat tcggtactgc agtttaagtt
     2701 tgcggatgca gccgccagcg caacacgcgt agtcatcaat acgacattcg gaatgggtgg
     2761 tattgtggat ttagcgagtg atattagtcg cgttagtgat attgatatta gtaaacgaaa
     2821 tgaagatttc ggtcaaacat tagggttcta tggcgttggt ccggggcctt atctggtgat
     2881 tccattctgg ggaccgagtt cagttcgtga tgcatttggt gtgatggtag atagttcgct
     2941 tttccatcca ttctggtcaa tttatcccga ttttgatttc ttaacggttc ggttgcctac
     3001 tgcagtgggt tggtcgattg atacgcgtgc gcaaatgctc gatattgata aaacgttgga
     3061 agaagcttcg ctggataaat atgaatttgt gcgagacgct tacttgcagc ggcgtagaag
     3121 ccttgtgcat gatggtaatc cgcccaagga agaacaaaat ggtgagaacg gagaataaat
     3181 ttcgcaccaa agaagttctg gcataagttg ttttgttgtt aataaaaagg cctgaatgat
     3241 ttatcattca ggcctttatt tttttaagtt gagttctcta ctgtaattat tatgcaatcg
     3301 tatcgccacg aagtgctgca atcctttcat caagcggtgg gtgcgacata aataaccgac
     3361 ttatctgact ttgttttccc gaaataccaa aagtagcgat cttatctggt aggtgtgaag
     3421 gttcgtattg ttgtttaagc cgttccagtg cagcgatcat tttttgtcgg cccgctaatt
     3481 tagcgccacc cgcatctgcg cgaaactcac gttcccgact gaaccacatg acgatgatgc
     3541 tcgccaaaat acccaatacg atttgagcaa taatactcgt gatcatgtaa gcggggccgt
     3601 gctcttcttc tgttttgaag atgaggcgat caacggcatg gccaatgatt cgggataaga
     3661 aaattacaaa agtatttacc acgccctgaa tcaaagtaag cgtgaccata tcaccattcg
     3721 caatatggct cacttcatgc gctagcactg cttcagcttc atcttgctgc atgttggcta
     3781 atagtccggt actcaccgcc actagtgcgt tgttccgact cattcccgta gcaaaagcgt
     3841 taacatcggg cgaatcatag attgctacct ctggcatgcc gataccagca gcatgcgctt
     3901 gtttgcgtac ggtttcaact aaccagccct ctgttgcatt ggatggaatc tcgatgacct
     3961 gagcaccagt catgtgtttg gctgaccatt tggacatggc gagtgacatc agagatccac
     4021 caaagcccaa taccgcagag aaaaccagta aagcactaaa atttagaccg ccgccttgcg
     4081 cgtcaagtac tcgatctacg ccaagtattc tgagcgttat actcagtacg ataagaatag
     4141 caagattagt tgctataaat aacagtattc gtttcataac atattaccta ttcaatagtg
     4201 agataaaaat tattggaatc atctacattt ttttggttca atttgactct attgtagata
     4261 ggggtgagta gttattttat caagcatagt ggttaaagag aaacagttca tccctgaaaa
     4321 taaaactaag cgggttgagg tacaatacag atcaaatgat aaagacaaca ctgattttta
     4381 aaacgatgga gatcgaatga gtcaacaacc tgttgtgctg ataggaagtg ggttagccgg
     4441 ttatggcgta gctcgggagc tgcgcaaact caattcagaa ataccaatta cgatgttatc
     4501 agcggatcat ggcggttttt attccaagcc catgttatca aatgcgatag caacgggtaa
     4561 aacccctgaa tcgattttga attctagtgc agtgcaaatg gccaatcagc ttcatattac
     4621 aattcgacct gatacgaggg ttagtgtgat tgattcagct caaaaagcga ttgtgcttga
     4681 gaatggcgaa cacttgatct atggccagct agtgctagcg atgggagcag atcagattcg
     4741 tttgccgctg caaggagatg gcgttgcgga tctattaacc gttaatgatt tggatgacta
     4801 ccgccatttt cgggttgcat tagaaggaaa aaaacaaatt gctattctag gtgcgggctt
     4861 gatcggatgt gaatttgcca atgatctggt cggcgcaggg tatgaagtgc atgtgattga
     4921 tattagcacg caaccactgg gtcgtttgtt gccctccgaa ggggggcgtt ttttacagga
     4981 aaaattgaca gcaattggga tcaaatttca tttgaaaacc ataactcagc ggattgaccg
     5041 aatgggagat cgattaagct tgtcgtttga tgatgggact cagcttgaag tcgatttgat
     5101 tttatctgcg gtaggattgc gccctcgtat ccagttagct caagcagcgg gcattgaggt
     5161 caatcgcggt atcgtaacgg atcgttacct tcaaactcgt accccagata tctatgcatt
     5221 aggagattgt gctgaagtag aaggaaaagt gttaccgttt attatgccga tcagccatgc
     5281 atcacgtgct ttagcggtaa cactgaatgg tacgccgact ttgttgcgtt atcctgcgat
     5341 gccagtattg gttaaaacgc cagcttgtcc gaccattgtt tctccacctg accctgataa
     5401 agaaggggaa tggcgtgttg ataaaacaga agatggaatt aaagcattat ttcaggatat
     5461 cacaggcaat ttattgggtt ttgcattgct gggtgatgcg accaaagaaa aaaatgcttt
     5521 gactccttta ctccctgcgg tgcttgattg attctcagcg cagaagttga tcaattaata
     5581 ctgattattg aattagaacg agactaaagc attctgagat tgggtgatag atgttattgc
     5641 aattaaaacc aggttgggga ttgtgttggc tgagtagtgt taatcgatta agtcgagtac
     5701 tgtttagcaa tgtctagccg caattcagga tggcatcctg ttcctgtctc agcttcatct
     5761 gcgcttcgat ggtggttact caatcaacat tcattaactg atttaatcaa gcagcgttgt
     5821 aatcaatttc gtgttgagcc cactttccaa gcgttggcta gggcatgcca tgacgagcta
     5881 agtgtaattg acttgcaccg caatgaactc gcgttgattc gtgaggttta tttatattgc
     5941 ggggataggc cagtagtgtt tgcccattcg gtgattgcgc ggaaccattt acagggagta
     6001 tggcgcggat taagtcgctt aggcaatcag tcactgggtt cgttgctgtt cagtaatcct
     6061 ttgattaagc ggcaagatcg atttagcttt aggcagctta atgatcatca tttgctttat
     6121 catcgtgctt gtcaaagaat gtcaccaaac aaaccaagac gattgtgggc gcggcgtaga
     6181 tcgttctcac tatatggtca acctatttta gttactgaaa tttttttacc tggtatttta
     6241 gagctatgag tttgattacc aaagaacgtc ttattgctta tgcgagacta attcgactgg
     6301 ataagccgat tggtatcttg ttgttactat ggccaacttt atgggggatt tggttagcgg
     6361 ctgaaggtac gccggatgcg atgattctgt ttatctttat agcgggcacg atactgatgc
     6421 gttcggctgg ctgcgccatt aatgatttcg cagatcgacg gatcgatccg catgttgagc
     6481 ggaccaaaaa ccgaccgtta gcagtgggcg tgattactgc gaaagaagca gtagtagttg
     6541 caagcgggtt aagtttttgc gcgtttctat tagtattgcc gctcaacacc ttgacacttc
     6601 agctttcagt ttgtgccctt ttcctggcag ggacttatcc gttcacaaaa cggtttttcg
     6661 caatgcctca ggcttatctt ggaattgcct ttagttttgg aattcccatg gcttttgctg
     6721 cacaaacagg agaattgccg ccgattgttt ggattttgtt ggtggccaat gtgttgtggg
     6781 ttatcgccta tgataccgaa tatgcgatgg ttgatcgtgc agatgatctc aaaattggaa
     6841 ttaaaacatc ggcaattacg ctgggacgtt acgacatcat cggtattatg ctgtgccata
     6901 gtttttttct ggggataatg acttatgttg gctgggtatt gcagctcggt gttctctatt
     6961 atctatctct cggtgtagtg gtcgcattgg tcatttatca ataccaattg atcaaggatc
     7021 ggaaaccgcc gggttgtttt aaagctttct tgcataataa ttgggttggg gcttcgatat
     7081 ttgctggaat tgctttggat tcgttaatca aaataccctt ttaagcaagt gactgcgagg
     7141 tttcgatgaa atataaggat ttgcgtgatt ttttagacca actggcactg cggggtcagc
     7201 tgaaacgggt gacgcatccc atcgatccct atttggagat gacagagatt tgcgatcgtt
     7261 tattgaaaca agggggcccc gctgttttat ttgagcaacc taaaaatcat tcgataccgg
     7321 tactcggaaa tttgtttggt actcccgatc gagttgcgct aggaatgggg caagaatcga
     7381 tggcagcttt acgagaggtt gggaaactat tggcttacct caaggagccc gatccaccga
     7441 aaggattgaa ggatgcttgg gagaaactgc cggttcttaa gcaagttttg aatatggcac
     7501 ctaaagtggt atccagcgcc ccctgccaag aaatcgtgtg ggaaggccaa gcggttgatt
     7561 tatcgaaatt accgattcaa acctgttggc ctggcgatgt tgcaccgttg attacctggg
     7621 ggcttacgat tacgcgaggt ccccataaat ctcgccagaa tctgggaatt tatcgtcagc
     7681 aagtgattgc gcctaataaa gtaatcatgc gctggctcgc gcatcgcggt ggagcgctgg
     7741 actatcgtga cttttgccaa gttaatcccg gcatgcctta tcctgttgcc gtagcactag
     7801 gtgctgatcc tgccacgata ctgggtgcgg tgacacctgt tccggatagt ttgagtgagt
     7861 atcaatttgc aggactatta cgcggtgcca agacggaggt tgtgcagtgt ttaacccatg
     7921 atttacaggt acctgcgagt gctgaaattg ttttggaggg gtttatttac ccggatgaaa
     7981 ccgcaattga aggtccttat ggtgatcata ccggctacta caatgagcaa gagcgctttc
     8041 ctgtgtttac aatcgaacgc atgacgatgc ggcgtaatcc tatatatcat tcgacttata
     8101 ccggtaaacc gccggatgaa cctgcgattt tgggggtggc ccttaatgag gtgttcgtgc
     8161 cgctattaca aaaacaattt actgaaatta cggatttcta tttgccgcca gaaggttgtt
     8221 cttaccgcat ggcaattgtt agcatgaaga agcagtatcc ggggcatgca aagcgtgtgt
     8281 tgtttggcgt ttggagcttt cttcgccaat tcatgtatac caaattcatt atcgtaacgg
     8341 atgaagatat cgatattcgt gactggaaag aggtaatttg ggcgattacg acacgcgttg
     8401 atccagcaag ggataccttg atcgtcgaaa atacccccat cgattattta gattttgcta
     8461 gccctgtttc tggattaggg gggaaaatgg ggctagatgc taccaacaag tggcctggag
     8521 aaacagtgcg tgagtggggt agaacgatta ctatggatgc ggcggttaaa tcacggattg
     8581 atcagctttg ggatgaatta cctttctagg ttaaaagtta cccaataatt tatcctcatt
     8641 gctaaccttt aaatcaccag tctaattgta ctgaaagtgg ctgagcccat tgagtactgg
     8701 atttgtgatt gatattccat cacgatacag gtgacatgtt agaatatggc ctttttatgc
     8761 agcttttata ggcaatatcg atctaaccga gaaacggatt ccagaatacg gcattcattt
     8821 tttgctaaaa tagttgctta ttatcaatca gtttattttt agtggagtta tttaatggct
     8881 atagaaagaa cgctgtcgat tatcaaacca gacgcagtgg caaaaaatgt tattggtcaa
     8941 atttataccc ggtttgagaa cgctggatta aagattattg cagcgaaaat gatgttgctt
     9001 tcccgaacag aagcagaaac attttatgct gttcaccggc aacgtccttt ttttaacgat
     9061 ttagtcagct ttatgacttc agggcccgtc atggtacagg tactagaagg ggaagatgca
     9121 atcaaaaaga atcgtgaatt aatgggagca accgatccta agaaagcgga taaagggacg
     9181 attcgtgctg attttgcaga tagtatcgat gccaatgcgg tgcatggttc agatgccgca
     9241 gaaacagcaa gtgtcgaaat tgcttgtttt ttcccagcat tatctatttt ctctcgttaa
     9301 ttcgatcaat tactgcaaac gcatttgtta agtatgaatt cggttgttaa tttgcttgat
     9361 tacgattcag ctggtcttgt tcgtttctgc gaagaaatcg gcgaaaagcc ttatcgtgcc
     9421 aagcaattac tcagatggat tcatcaattt gggaaatccg attttgcaga aatgagcgat
     9481 ttggcaaaat tattccgcga aaaacttcat tcattagcga cgattcgttc accggaagta
     9541 atgagtgatt acacggcgca agatggaact cggaaatggt tgttagcgac ggggcagggt
     9601 aatgcagttg agatggttta tattcctgaa gtagcgcgga acacattgtg catatccagt
     9661 caggttggtt gtgcacttaa ttgt
//
LOCUS       DAOQLJ010000085         9666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2748_length_9666_cov_7.192072, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000085 DAOQLJ010000000
VERSION     DAOQLJ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9666
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2748_length_9666_cov_7.192072"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..1512
                     /locus_tag="PK522_07130"
     CDS             310..1512
                     /locus_tag="PK522_07130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNC41544.1"
                     /translation="MRNNKTKKVFLKAGKEKSLLRRHPWVFSGAVDRTENLPTLSDTV
                     HIFSAQGEFLAQAAYHPQAQIAARVWSWEQELSIDADFIHQRIAQAVAFRRQMGFAKL
                     STGMRIIHGESDGLPGLVVDQYDRVVVMQISSVGIERWREACIKSLRDEFDPICIYER
                     SDTEVRVLESLPERCGVVFGDLPANITIKEHGLSFQIDVSSGQKTGFYLDQRDNRALV
                     GALAADKDVLNCFCYSGGFTVYAKQAGARSVLSIDASQSALTLAMQNVALNGFATDES
                     EWVCADVFQALRKLRDQNRSFDLIILDPPKFAPTASFAEKAARGYKDINLLGFKLLRP
                     GGILASYSCSSGISSDLFQKIIAGAALDAGVEAHIIAYQNAAADHPVLLSFPEGAYLK
                     GLILKKRL"
     gene            1613..1936
                     /locus_tag="PK522_07135"
     CDS             1613..1936
                     /locus_tag="PK522_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3470 domain-containing protein"
                     /protein_id="HNC41545.1"
                     /translation="MTYVVTESCIKCKYTDCVDVCPVDCFREGPNFLVIDPDECIDCT
                     LCVAECPVEAIYAEDDVPDNQRDFIALNGELSKLWKPIIEKKDALADADDWAKVTDKV
                     DHLER"
     gene            complement(2067..3164)
                     /locus_tag="PK522_07140"
     CDS             complement(2067..3164)
                     /locus_tag="PK522_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNC41546.1"
                     /translation="MKRREFIKWVGMGATLLPTSTTILAQSLIPNQWRGYRLTYQVTL
                     PPQGKQAHLWLPLPDSIDTPRQLIQGSVWNGTTKAGKLFTLPKTDTNLFHAEWQGFSE
                     RTVTVSSIVKTADRKVDLRFYTPSAKSSYPENIKRFLQPTQHIVIDGAIRKLADSIIK
                     KEEIRSPLNKARAIYDWVIDHSQFDKNIRGVGKGDIKQITINDPMLGKSADISALFVA
                     LARAVGIPARQQFGLRIDESAIHHDLGKLGDITTAQHCRAGFFLAELGWIPVDPAEVC
                     EVIALENLAEQPEKIAALKERLFGSWEMNWIALNDDEDVSLSHGKLTTKLPFLIYPHA
                     LIDGEPIDSLNPNEFSYKITSALLVGTGGKF"
     gene            3248..3928
                     /locus_tag="PK522_07145"
     CDS             3248..3928
                     /locus_tag="PK522_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNC41547.1"
                     /translation="MSTHFSNPLATIELTTGSNPTHSIIWMHGLGADGNDFVPIIEEL
                     DLPKTIAIRFIFPHAPKQPVTVNGGFIMRAWYDIRSADLDHAEDEVGLKRSEQLIMQL
                     IEKEKSRGIASENIVLAGFSQGGVMSLFVGLRYAEKLGGVMVLSGYLPLAHQFESSAH
                     LVNHTTPIFIGHGREDMIVPMQLANKAKQQLIVKGYLVEWHEYNMAHSVCNDELADIS
                     KWLRRVLL"
     gene            complement(3988..6096)
                     /locus_tag="PK522_07150"
     CDS             complement(3988..6096)
                     /locus_tag="PK522_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family decaheme-associated outer
                     membrane protein"
                     /protein_id="HNC41548.1"
                     /translation="MKQRNIFHLSTIAAALIITMPLETLGDDTIKQMTKPQSTIQFGA
                     GYLSDDNARFGQFNGLRDEGAYGIFNVDILRRNDDSGTWFKIIGRNLGLQNREIRLEH
                     SKQGNWGYSIEFNQTPRYEPFTAITAVEGIGSSQLEVPTTPTTGNPTQLKTEREAISV
                     GINKILPGNFELNLHFRNEEKDGSRIFGRGNRLGAPSPTAVGGFEFTPEPINFTTRQF
                     GATLDYTGKDLQVTGGYHGSIFLNKNSALHINGGSDVGGAYAPSNFSPIALPPDNQAH
                     QAFLSGGYSFTPTTRGTLKASYTRATQSDSFFPTQLSTPGIGSNLGGQVDTALVQSSL
                     TARPLPKLSLLANARFEDRDDKTPVFLYNPMALELDFNGNRWSGLNQPRSFRTWNSKF
                     ESSYVLPMNFRIIGGIDYEHWDRTGLPATTGHRSTTDEISYRAELRRTLAETVTGTIS
                     YIHSDRFGSHFLTNRTATGDPFFNFIAPINLADRKRDKVRLSINWEPIDPLSVQVMID
                     ESWDNYGHRAGSDLGVQKGSARNYSIDAVYTFSEAWQATGWFSRNETFLDQASLTPAP
                     PRQLQERWTVGLENISTAFGAGLNGKPTDKIDVGANLEYSDITDKFNQSVITNAQTYT
                     APNINFQLARFRLFARYAIQRNYGIRLDYILDHFKTNEWTWNSNIPAQNWVYTDGTQL
                     TQDRSQLINFVGFSAYYSWQ"
     gene            complement(6117..7076)
                     /locus_tag="PK522_07155"
     CDS             complement(6117..7076)
                     /locus_tag="PK522_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HNC41549.1"
                     /translation="MSRQIKKGLLHLLPMLVLALALGISPNSEAKSASEDADDESERG
                     IVLRKDRECTDCHDESSEYPILAIGKTKHGTTADSRTPDSCKSCHGESNDHLDGETTM
                     PMPSITFGKNSKNPIDERNQICLNCHQGGKRMHWLSSTHANRDTACTACHQIHAAQDK
                     VRDRIAQAEVCFACHKEQRAQINRPSRHPIREGKVVCSDCHNPHGSAGPSKMIRDSVN
                     ETCFSCHMEKRGPFIYNHPPVQENCAICHNPHGTTAPNLLKVRSPFLCEQCHEPNTHQ
                     SSPATLTGYYRRNTLARGCMNCHTQIHGSNNPENIRNEEVFQR"
     gene            7279..8389
                     /gene="prfB"
                     /locus_tag="PK522_07160"
     CDS             join(7279..7359,7361..8389)
                     /gene="prfB"
                     /locus_tag="PK522_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964773.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNC41550.1"
                     /translation="MEAEQLNTLRNQLVELNDRQKSLRRYLDFDNKKLRLIEVSRHAE
                     DPAVWNDAQRAQEVGREKKSLENVVLLLESLEKQLQDADELLQIAIEENDATAFQSIV
                     ADVAQLEKTIADMEFRRMFSNPMDPCNCFVDIQSGSGGTEAQDWAQMLERMYLRYCER
                     HGFEVELLEESAGDTAGIKSATLKVSGEYAYGYLRTETGVHRLVRKSPFDSGNRRHTS
                     FASVFVYPEVDDSIDIEINPAELRIDTFRASGAGGQHINKTDSAVRITHLPTNIVVQC
                     QSGRSQHRNKADAMAMLKSRLYEAELRKRNETKQAVEDAKTDIAWGYQIRSYVLDQSR
                     IKDLRTNVEIGNTQAVLDGDLDDFIQASLKQGVAS"
     gene            complement(8449..>9666)
                     /locus_tag="PK522_07165"
     CDS             complement(8449..>9666)
                     /locus_tag="PK522_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HNC41551.1"
                     /translation="MTFLGQFLDHDMTLDITSSLEQQVDPEMIQNFRTPTFALDSIYG
                     TGPSGSPYLFDQTIDHGFTTLLLEPITGSETKSRDGSLKYDLPRNSQNTPLIGDPRND
                     ENLVLSQMQVAFIKFHNNLVIRLKAEGLTNPVEIFLEAQRLTRWHYQWIILHEFLPKT
                     IGQALVDDILQNGRKFYQWNNAPYIPVEFSVAAYRFGHSQVRPSYRVNFGTNDANQIF
                     ALFFNDNLTNPVTIDMRGGSRAPERFIDWQTFFDFGDNNARNNKRIDTKISTVMFDLP
                     GMPANEPQSLAQRNLLRHLTFKVPSGQRVAAAMGIPALLENDLADLKPFNLHNRTPLW
                     FYVLREADVKADAKRLGPVGGRIVGEVMIGLLQGDNTSYLSQDPDWTPHLGSNGNFTM
                     VDLLKFAGVVTTL"
BASE COUNT         2630 a         1910 c         2289 g         2837 t
ORIGIN      
        1 gctcgaacct gggacctgcg gattaacagt ccgtcgctct accgactgag ctatcaggga
       61 atggaagcga tgcttatagt aacgctttga tattcattgg tcaacttcgt tgtgcgctgt
      121 tcttatcagg aaacagactt tatctaaatg taaaatacat attttttcgt gaattgaatg
      181 agttatattc aaatcaaaga aaaaagaggc atgatcaaat tattttggga aggatatgtc
      241 gcttcgggat atgatcacct agttattagc ttgtctgctg aagcaaaggc taaattttga
      301 agagactgca tgagaaacaa taaaactaaa aaagtattcc ttaaagcagg aaaagagaaa
      361 tcattattac gtcgccatcc ttgggttttt tcgggtgcag ttgaccgtac ggaaaattta
      421 ccaacattga gtgataccgt acatattttt tctgcgcagg gtgaatttct agcgcaggct
      481 gcttatcatc cgcaggcaca aattgccgct cgggtttggt cttgggagca agaattatca
      541 atcgatgctg attttattca tcaaagaatt gctcaagccg ttgctttccg ccgtcaaatg
      601 ggttttgcta aactaagtac agggatgcgc atcattcatg gtgaatctga tgggttgcct
      661 gggcttgtgg ttgatcagta tgatcgggtg gtagttatgc aaatcagcag cgttggtatc
      721 gaacgctggc gggaggcctg tattaaaagc ttgcgggacg aatttgatcc tatttgtatt
      781 tatgagcgtt cagatactga agtgcgggta cttgagtcgt tgcctgaacg atgtggtgtt
      841 gtattcggtg atttaccagc taacataacc attaaagagc atggtttgtc ttttcagatt
      901 gatgtcagta gtgggcaaaa aacggggttt tatttagatc agcgcgataa ccgtgccttg
      961 gttggtgcac tagcggcaga taaagatgtg ctcaattgct tctgttacag cgggggattc
     1021 acagtttatg caaagcaagc aggggcacgc tcagtgcttt cgatcgatgc ctcgcagtcg
     1081 gcgctgacac ttgcgatgca aaatgttgcg ctcaatggat ttgcgaccga tgagagtgaa
     1141 tgggtttgtg cagatgtttt tcaagcgtta cgaaaattaa gagatcaaaa tcgtagcttt
     1201 gacttaatta ttttagaccc cccaaaattt gctcctacag caagttttgc cgaaaaagca
     1261 gcgcgtgggt ataaggatat taatttgttg gggttcaaat tgcttagacc aggtggaatc
     1321 ttagcaagtt attcatgctc cagtggcatc agtagtgatt tatttcaaaa aatcatcgca
     1381 ggagcagccc tagatgcagg agttgaagcg catatcattg cttaccagaa tgcagcagct
     1441 gatcatccgg tattgcttag ttttcctgag ggggcttatc tcaaaggctt aattttaaaa
     1501 aagagactat aaatacgttt tgatcgtagc gtaggagggt agaagtccgc ttcgattttc
     1561 tgataaaatt atatattctg atattaaaaa gtgccttgca aggaatttca tcatgactta
     1621 tgtagtaact gaaagttgta ttaaatgtaa atatacggat tgcgtggatg tatgcccggt
     1681 tgactgtttt cgtgaaggac ctaatttttt agtcatcgat ccagatgagt gtattgattg
     1741 cacgctttgt gtcgcggaat gtcctgttga agcgatttat gctgaagatg atgtgcccga
     1801 taatcagcgc gatttcattg cgttaaatgg cgaattatct aagttgtgga aaccgatcat
     1861 cgagaaaaaa gatgcgctag ctgatgcgga tgattgggca aaagtgaccg ataaagtcga
     1921 tcatttagag cgttaagatt atttcttaaa ttaattttac gtaaagaatt gtcaatcact
     1981 ggtaggtaac cagtttctat tatgaatcaa ttactaccga tgttttaaga ttgtagtaat
     2041 tgattcatgc aatatagcgt ttctttttag aatttaccgc cggtacccac taataaggct
     2101 gaagtgattt tatagctaaa ttcattggga ttgaggctat caattggttc accatcaatc
     2161 aaagcatgtg gataaataag aaatggcagc ttagtggtaa gttttccatg gcttaggctt
     2221 acatcttcat catcattcag tgcgatccag ttcatttccc aagagccaaa taatctttct
     2281 ttaagtgctg cgattttttc gggttgctca gcaaggtttt ctaatgcgat gacttcacat
     2341 acttctgcag gatcaactgg aatccatcca agttctgcga gaaagaatcc ggcacggcag
     2401 tgttgagcag ttgtaatatc gcctaatttt cctaaatcat gatgaatggc agactcatca
     2461 atccgtaagc caaattgctg acgtgctgga atacccactg ctcgcgctag cgctacaaac
     2521 aacgcgctga tatcggcact cttaccaagc atagggtcgt ttatagtgat ttgtttgata
     2581 tcgccttttc ctaccccgcg gatattttta tcaaattgtg agtgatcaat gacccaatcg
     2641 taaattgctc tcgctttatt gagtggagag cgaatttctt ctttcttaat gatcgagtcg
     2701 gctaatttac gaatggctcc atcgattacg atgtgttggg taggctggag gaaacgcttg
     2761 atattctccg ggtagctact ttttgcagac ggtgtataga atcgcaaatc aactttgcga
     2821 tcggctgttt ttacaatgct actgacggtt acggtgcgtt cgctaaaacc ttgccattca
     2881 gcatggaata aattcgtatc agttttaggt agtgtaaaaa gttttccagc tttagttgtg
     2941 ccattccaaa cgcttccttg tattaattga cgcggagtat caatgctgtc ggggagcggt
     3001 aaccagagat gggcttgctt tccttgtggt ggtaaggtta cttgataggt taaccgatac
     3061 ccacgccatt ggttcggaat taacgattgc gcaagaatgg ttgtacttgt ggggagtagc
     3121 gtagctccca taccaaccca tttaataaat tctctgcgct tcatacctgt ctcctctgtt
     3181 attaagttta gtatagaatt ttcaaatttg aattctacta ttttaccagg gcgagttcct
     3241 tcaattcatg tctactcact tctcaaatcc gttagcaacg atcgaattaa caacaggctc
     3301 aaatccgact cactcaatca tctggatgca tgggcttggt gctgatggca acgatttcgt
     3361 tcccattatt gaagagctcg atctccctaa aacaatagcc atccgattta tctttccgca
     3421 tgcacccaag caaccggtca cggtcaatgg tgggtttatc atgcgcgcct ggtacgacat
     3481 acgcagcgct gatttagatc atgctgaaga tgaggtaggg ctaaaacgat ctgagcaatt
     3541 gattatgcaa ctgattgaaa aagagaaatc acgcggaatt gcatcggaaa atatcgttct
     3601 ggcgggattc tcacaagggg gggtaatgtc gctttttgtc gggttgcgat atgctgagaa
     3661 attgggtggt gtgatggtac tgtccggtta cttgccactt gcccatcaat tcgagagcag
     3721 cgcccatctt gttaatcaca ctacaccgat atttatcgga catggccgcg aggatatgat
     3781 cgtaccgatg cagcttgcga ataaagccaa gcagcaactc attgtaaaag gctatcttgt
     3841 ggaatggcat gagtacaaca tggcccattc agtttgcaac gatgaactcg cggatatcag
     3901 taaatggctg cggcgcgttt tattgtagtg cattctgaag agagcattta ccagggtaaa
     3961 gatcgtgttt tcagaatgca cggatgatta ttgccagctg taataagcgg agaatcctac
     4021 aaaattaatc aattgactgc gatcttgcgt tagctgcgtt ccatcggtat agacccaatt
     4081 ctgagcaggg atatttgagt tccaagtcca ttcgttggtt ttgaaatgat cgaggatgta
     4141 atccaagcga attccatagt ttctttgaat cgcgtatcta gcaaataatc taaatcgagc
     4201 tagctggaaa ttgatgttcg gggccgtata agtttgcgca ttggtaatca ccgactggtt
     4261 aaatttgtca gtaatatcgc tatactcgag attagcaccg acatcgattt tgtcagtggg
     4321 tttgccattt aatccagcac caaaagcagt gctaatgttc tctaggccaa ctgtccaacg
     4381 ttcttggagc tgacgaggtg gggcaggagt cagtgaggct tgatcgagaa atgtttcatt
     4441 tcgtgaaaac catccggttg cttgccaggc ttcagagaaa gtataaacgg catcgattga
     4501 ataatttctt gcagagcctt tttgaacccc taagtctgag cccgcgcgat gaccgtagtt
     4561 atcccatgat tcatcaatca tgacttgaac cgaaagggga tcaatcggct cccagttgat
     4621 ggataatcta actttatcgc gtttccgatc ggctagattg atgggggcga tgaaattaaa
     4681 aaagggatca ccagtagccg ttcgatttgt cagaaaatgt gaaccaaagc gatcgctatg
     4741 gatgtaggaa attgttcctg ttacggtctc agcgagtgtg cgtcgcaatt cagctcgata
     4801 cgatatttcg tcagttgtgc tgcgatggcc tgtggttgcc ggtaatcccg ttcgatccca
     4861 atgctcatag tcgataccac caataattcg gaaattcatg ggtaatacat aactgctctc
     4921 aaatttactg ttccaggttc taaaggatcg aggttggtta aggcctgacc aacgattgcc
     4981 attgaagtcc agttctagtg ccatagggtt gtaaaggaaa acaggtgtct tatcgtcacg
     5041 gtcttcaaat cgtgcatttg caagcaatga aagcttgggt aaagggcgag cggttaaact
     5101 actctgtaca agggctgtat cgacctgtcc tcccaaatta cttccgatac caggcgtgga
     5161 gagctgggta ggaaaaaatg aatcggattg cgttgcacgt gtataagagg ccttcagtgt
     5221 tccacgtgtt gtcggagtaa agctatagcc accggataaa aaagcttgat gtgcctgatt
     5281 atcagggggg agcgctatgg gtgaaaagtt actcggcgca taagctccac caacatcact
     5341 gcccccattg atatgaagtg cagaattttt attcaggaaa attgaaccat ggtaaccgcc
     5401 tgttacttgc aaatctttac ccgtgtaatc caatgtcgcc ccaaattgac gcgtcgtaaa
     5461 attaattggc tcaggtgtaa actcaaaacc gccgacagca gtggggctag gtgctcccag
     5521 tcggtttcca cggccaaaaa tacgtgagcc atctttttct tcgttacgga aatgaagatt
     5581 cagttcaaaa tttccaggta agattttatt gatacccacg ctgatcgctt ccctttccgt
     5641 tttgagctga gtaggattac ctgtcgttgg agtcgttggt acttccagtt gcgaacttcc
     5701 aataccttcg actgctgtta tggcagtaaa aggttcataa cgcggtgttt gattaaactc
     5761 gatagaatat ccccagtttc cttgtttact atgttcgaga cgtatctcgc gattttgtaa
     5821 acccagatta cgacctatta ttttgaacca ggtgccacta tcatcgtttc ttcttaagat
     5881 atcaacattg aaaatgccat aggccccctc atctctcaaa ccattaaatt gtccaaagcg
     5941 ggcgttatca tcggataaat aaccagcacc aaattgaatc gtactttgag gtttagtcat
     6001 ttgcttgatg gtatcatcgc ctaacgtttc aagtggcatt gtgatgatta gcgctgcagc
     6061 aatcgtgctg aggtgaaaaa tatttcgctg tttcatgatt cattcctcct tgtcgattaa
     6121 cgctggaaga cttcttcatt gcgaatattc tcagggttgt tactgccatg aatttgcgtg
     6181 tggcaattca tgcatcccct ggctagagta ttccgacggt aatagcccgt aagggtggct
     6241 gggctacttt gatgcgtgtt cggttcgtgg cattgttcgc agagaaaagg agaacgtact
     6301 ttcagtaaat tcggtgccgt tgttccatga ggattatggc aaatggcaca gttttcctgg
     6361 acgggtgggt ggttataaat aaagggtcct cgtttttcca tatgacagga aaagcaggtt
     6421 tcgttgacac tatctctaat cattttgctt ggccctgctg aaccatgagg gttatggcaa
     6481 tcggaacaca cgactttgcc ttcacgaatc gggtgtcttg aaggtcgatt gatttgagct
     6541 cgctgttctt tatggcacgc aaaacatact tcggcttgag caattcggtc acgaactttg
     6601 tcttgcgctg catgaatttg atgacaggcg gtgcaagccg tatctcggtt tgcatgggtg
     6661 ctgctcaacc aatgcatgcg tttaccgcct tggtgacaat ttagacagat ttgatttctt
     6721 tcatcgatag ggtttttgga gttttttcca aacgtaatcg atggcatggg catcgtggtt
     6781 tcaccatcca aatgatcatt actttcacca tgacagcttt tgcaggagtc tggtgttctg
     6841 ctatccgctg ttgtgccatg tttagtctta ccaatagcaa gaattggata ctcactgctt
     6901 tcatcgtggc agtcagtaca ttctctgtct ttcctgagga caataccgcg ttccgattcg
     6961 tcatcagcat cttcactggc gctttttgct tcagaattgg gggatatccc gagtgctagt
     7021 gctaacacta gcatcggtag taggtgtaat aagccttttt ttatttgtct tgacatgatg
     7081 gatagcctct ctaagttagt taaggttagg gttgaagccg tttcttgctt caacaaaaga
     7141 tgaaaaaatt atctaactgg agggctattc cgatctgtgt gatatctcac gatctgagaa
     7201 ataaattcaa ttgcagtatc gtttaacgag gggttagcca aacctgtata attttgtatt
     7261 ttttgggtgg caatcatgat ggaagcagaa caactcaata cgcttagaaa ccaattagtc
     7321 gagctgaacg atcgccaaaa atcgttaagg aggtatcttt gacttcgata ataagaagct
     7381 acggttgatt gaagtctcta gacatgcgga ggacccagct gtctggaatg atgcgcagcg
     7441 agcccaagaa gtagggcgtg aaaagaaatc gcttgagaat gtagtgttac tactcgagtc
     7501 attagaaaaa caactgcaag atgcggatga actccttcaa attgcaatcg aggaaaacga
     7561 cgcaacggcg tttcagagca tcgtcgccga tgttgcgcaa ctcgaaaaaa ccattgcaga
     7621 tatggaattc cggagaatgt tttccaatcc aatggatcct tgcaattgtt ttgtagatat
     7681 ccaatcgggt tccggaggga cagaggcgca agattgggcg caaatgctag aacggatgta
     7741 tttgcgctat tgcgagcgcc atggttttga ggtggaatta ttagaagaat cggctgggga
     7801 tacggcaggt ataaaaagtg caacgcttaa agtatccggt gaatatgctt atggctattt
     7861 acggactgaa acgggagtcc atcgtttggt cagaaaatct ccgtttgact ctggtaaccg
     7921 gcgtcataca tcctttgcta gcgtgtttgt ttatccagaa gtcgatgaca gtattgatat
     7981 tgagataaat ccagcagaac tgcggatcga tacgttccgc gcatccggtg ctggcggtca
     8041 acatatcaat aaaaccgatt cagcggttag gattacccat cttccaacta atatcgtcgt
     8101 tcagtgccag agtgggcgct cgcagcatcg caataaagct gatgcgatgg cgatgctcaa
     8161 gtcgcgcctt tatgaagcgg aattgcgcaa gcgcaatgaa accaaacaag cggttgaaga
     8221 tgcaaaaaca gatatcgcgt ggggttatca gatacgttcc tatgtgcttg atcagtcgcg
     8281 tatcaaagat ctgcgcacca atgtcgaaat tggtaatacc caagcagtgc tggatggaga
     8341 tttggatgat ttcatccagg caagcttaaa acaaggggtt gcttcttgag atcggtgtga
     8401 ttagaaaaag ttgggttgat gatcaaccca acttgaaatg cgagatattt atagtgttgt
     8461 gacaacaccc gcaaatttga gtaaatctac catcgtaaag ttaccatttg aacctaaatg
     8521 aggtgtccaa tcggggtctt gagaaagata ggaagtattg tctccctgta aaagcccaat
     8581 catgacttcg ccgacaatac ggccgccaac tgggcctaac cgttttgcat cagctttcac
     8641 atcagcctca cgtaatacat agaaccacaa gggtgtgcga ttatggagat taaatggttt
     8701 tagatcagcc agatcgtttt caagtagtgc aggaattccc atggcagctg caacgcgttg
     8761 accagacgga actttgaatg tcaagtggcg cagtagattg cgttgcgcaa gggattgagg
     8821 ctcatttgca ggcatacctg gaagatcgaa cataactgta gagatttttg tgtcaatacg
     8881 cttattattg cgtgcgttgt tatcaccaaa atcaaaaaat gtttgccaat cgataaaacg
     8941 ttcgggtgct cttgaaccgc cacgcatgtc aatggtgacc gggtttgtta agttatcatt
     9001 gaaaaatagg gcaaaaatct gattagcgtc atttgtacca aaattgaccc gatagctggg
     9061 tcttacttgt gaatgcccaa aacgataggc agcaactgaa aactcgactg gaatataagg
     9121 ggcgttgttc cattggtaaa atttccgacc attttgcaat atatcatcta ccaaggcctg
     9181 accaattgtt ttgggtaaga actcatgcaa aataatccat tgataatgcc aacgcgtcaa
     9241 acgctgtgct tcaagaaaga tttcaaccgg attggttagc ccctctgctt tgagtctgat
     9301 taccaagtta ttatgaaatt tgatgaacgc gacttgcatt tgggacaaaa ctagattttc
     9361 atcattgcga ggatcgccaa tcagcggtgt attttgacta ttgcggggta gatcatattt
     9421 caaagatcca tcacgggatt tggtttcaga gccggtaatt ggttctagca acaaagtagt
     9481 aaatccatga tcgattgtct gatcaaatag ataaggtgag ccagatgggc ctgtaccata
     9541 gatactatct aatgcaaagg ttggtgttcg gaaattctga atcatttcag gatcgacttg
     9601 ctgctctagg ctagaagtaa tatcgagcgt catgtcatgg tcaagaaatt gaccaaggaa
     9661 agtcat
//
LOCUS       DAOQLJ010000086         9660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2753_length_9660_cov_7.620406, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000086 DAOQLJ010000000
VERSION     DAOQLJ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9660
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2753_length_9660_cov_7.620406"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(390..1625)
                     /locus_tag="PK522_07170"
                     /pseudo
     CDS             complement(390..1625)
                     /locus_tag="PK522_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
     gene            complement(1713..2516)
                     /locus_tag="PK522_07175"
     CDS             complement(1713..2516)
                     /locus_tag="PK522_07175"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC41552.1"
                     /translation="MKSLKKEKLLRLINTSGVGMRKPENEGGSNSLTAANEYYQQVEQ
                     FAQRIRKTNDVDEMIKILDTLLNETKGLHNNNEIQNTRELRHAENKIESLKYELEELR
                     ELVHTDQMTGTYNRRGLDNIFMREAARADRYENALCVVLIDLDDFKFINDTHGHLFGD
                     SALIHFATIAKKTIRPSDVIARYGGEEFLIILPDTRPEEAIWVMQRLQNNLSKNPLLD
                     TEETPIKITFSGGITLRKFQETQNTVIKRADKALYRAKQYGKNRIEIIA"
     gene            complement(2705..3337)
                     /locus_tag="PK522_07180"
     CDS             complement(2705..3337)
                     /locus_tag="PK522_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC41553.1"
                     /translation="MIRVMIADDHAIVRQGLKQILSETGDISVTGEAETGFQAVKVIR
                     QQEFDVMLLDISLPDRNGIEILKQIKKDRPNLMVLMLSMHNENEFALRALKAGASGYL
                     NKQSAPAQLVTAIRQVSEGHKYISPALAQELANAWTSEADRPLHENLSDREFQTLCLI
                     ASGKSLSEISIEMCLSPKTVSVYRARMMEKLKISNNSELVRYAIKHKLVD"
     gene            complement(3370..4857)
                     /locus_tag="PK522_07185"
     CDS             complement(3370..4857)
                     /locus_tag="PK522_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNC41554.1"
                     /translation="MSESLSILLVGFLNQSAQTIRKIFTDEDINFDSKLVGTYDEFLH
                     EIQKCHWDIIIANEATMPTGTHAILTYFSQVNQYLRIPVIIISNESNEQSAKDFLKAG
                     AYDFMLISNLVRLAPVIKKCAQELKSIQENRDTQISLQKSEARFRAIASNLPGLVFQF
                     LKKPDKSVAFPFVSEGVLMLLGLTAEELTVQPDLFYQMILPEDQYSYNEQMKASAKNF
                     STWNWEGKIRVKGDSDIKWISLRATPRQSNQGGILWDGIVLNITRNKLAEIELANSHA
                     QLAELSSYLQKIKEHERMHIAREIHDDIGGTLTAIKCELQSCIDIDKPRLPEFYQQKA
                     RSIEELVDYVIDSTRRISLDLRPGVLDVGIVASVYWQAREFSKRNHIPCEVKCESEDI
                     SLNDDLAVAIFRIFQETLTNITKHANASIVKVKLAEINNHVLLEVIDNGKGITDLEMK
                     KPNSFGIRGIRERCQQLGGDFCITGTKGKGTSALIKIPIETKKIS"
     gene            5123..6832
                     /locus_tag="PK522_07190"
     CDS             5123..6832
                     /locus_tag="PK522_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNC41555.1"
                     /translation="MPQIINTNIASLNAQRNLNGSQGMLNTALERLSSGLRINSAKDD
                     AAGLAISERMSTQIRGLNQAARNANDGISLSQTAEGALTEIGNNLQRIRELSVQAANA
                     TNTASDRAALQAEVTQLTSEIQRVASQTQFNGLNLLDGSFSTMNFQVGANANQTISAS
                     SGNFQTNAFGNYRIGGLAAFTPGGSGDLVAGTIGTNSDAFGNSVLLSGTTGDTSGING
                     ALAAGDFRISTGSGNFDVYYRAGASANEIATAINRTGSGVNASATTEVVLGADTGAGA
                     GFQQNTTYSFAISTDYSDPTNANTVDPKYTTVSFKTGGADDTADVDSGSYLSTAVQAF
                     NDASAKTGFTAEAVLTEDGYWALKLTNSQGNDLRLLNNSYDNTGTALDITVSDTAVLD
                     GDTGTADSLADTLAGGGLDATTGVWTNGNGAWYTGKVTLDSAKSFTVTTAVADVFQDA
                     GTPGTAAAGTYGAQLQAASAMDITTAEAATRTLAIVDSALTTVNDQRARFGAIQNRLE
                     STISNLQTSSENLSASRSRIRDADFAAETASMTRAQILQQAGVAMLSQANAIPNNVLS
                     LLR"
     gene            6913..7275
                     /locus_tag="PK522_07195"
     CDS             6913..7275
                     /locus_tag="PK522_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNC41556.1"
                     /translation="MMISSTTGMADFLQPVLPQQKVQKSEHMPAQTTVTPESNPVNKQ
                     NTIELDVKEAVNQIQHFTETLAPNLQFSIDEETGKTVVKIMDAQTQEVIRQFPSEEAI
                     SIARALGKIEGLLFHDKA"
     gene            7308..8735
                     /gene="fliD"
                     /locus_tag="PK522_07200"
     CDS             7308..8735
                     /gene="fliD"
                     /locus_tag="PK522_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNC41557.1"
                     /translation="MISSPGLGSGLDVNGIVGQLMAIEQRPLLLLTNKEATQQAQISA
                     YGSLKGALSSFQSSVKTLTNLSIFTGIKANVGDSTIATASASTNAAVGSYQIEVQNLA
                     QAQKIKSESFATTSTAIGSGALTIEFGTYNGDGTFTSNPTKISKTITIESDQTDLASI
                     RDSINEADAGITASIVNDGSGNRLVFASNNTGTDNALKITVSDDDGNNLDNAGLSKLA
                     YDATTGGTANMTQTVAAQNATMVIDGITISKSSNTIKDALEGVTFNLLKAAPGTTTSL
                     SISEDTSKIQTAVSAFVGAYNELVKTISDISRYDTANKQASVLTGDATVRTVESQIRG
                     VLNSFLPGAPSSLNSLSQIGINFQKDGTLQLDNAKLTSVINDSEKDLGSLFATNGFAV
                     KLDKLVDGMLKNNGLIDGRLDGISDTIKDIGKQREMLGRRLEDVEKRYRTQFTALDTM
                     IASMTQTSTFLQQQLSNLPKASSGN"
     gene            8784..9236
                     /gene="fliS"
                     /locus_tag="PK522_07205"
     CDS             8784..9236
                     /gene="fliS"
                     /locus_tag="PK522_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNC41558.1"
                     /translation="MSAKNYAINSYQRVGVESGVASADSHKLILMLFEGSINALNRAK
                     QEILHNNISAKGEMISKAISIIDNGLKASLDISVGGEIANNLQALYDYMTRQLLIANI
                     QNSTGTINEVIELLSQLQEAWRSIGNKELAKPNIHQISNTHALEPTDI"
     gene            9244..9570
                     /locus_tag="PK522_07210"
     CDS             9244..9570
                     /locus_tag="PK522_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HNC41559.1"
                     /translation="MDGVQVIFTYKAILTVTEKMLKAAQANNWEELISLEQECKLLTQ
                     ALVSNSNQIQLSDEMQKKKIDIIHRILDKDAKIRTITEPWMTHLQNLLINANQKRKLD
                     RLYSSD"
BASE COUNT         3010 a         1873 c         1824 g         2953 t
ORIGIN      
        1 tttcttgtat acaaaacctc cgccacggtt aagttaattt tgcaggaccg actaactagt
       61 cttccctgaa aaaggcactc gataattgcc aagaagtcga tccggttagt tgggcactaa
      121 agagatcaag ttaaggagtt attttctgta tgattatttt tataaataaa cgaggtacag
      181 aaaatgagca agaaacgcag aaaaaagtgg atcccaattt ttgggtagat gtcttggaag
      241 ttatttttcc taccatcttt cagtaaaaag attaatccat taccttaaaa tttagaattc
      301 tttcggtaat cctcacattt tttaacttgc gattccccgt ggtcttgcca cgaggttgtt
      361 tattaggatt atggcagagg taggaaatat taatgggctg tttcatttat tctatttgtc
      421 caactcagcg cttccagctc tgcacgagtt aactgaggta aggttaaaaa attaatttct
      481 ttaagtatac tttgcacttg agtaacatcg cctttctcgt tagcttcaat gagagcaagc
      541 tagcggccta gacgacccga tcgttgtaat aacgcttgag ttaattcctc gggaatattg
      601 agcttatcaa caatttgttg catttccatt ccaagtaaag tatctagaag cgagaaaata
      661 ccaaccataa aagcacgatc ttgatatgtt ttatcatgag ggcgatcaat ctgcgctata
      721 agctccatca attttccacg aactgcagca gtttgcatta atgcgttcga aatgctatct
      781 cccgatgaat cagaggtata taacaacaac tgaacccacc tttgaagttg ttgccggcct
      841 aaaattatta ctgcttgctt aatggaatta attctttgtt gtaagccaaa accaactgaa
      901 tttactaagc gtagtaagtt atagctaaga ttgggttgat gcttaagttc tgtttcaatc
      961 tcttctgcct cacaatcccc catcaccata gttaataaat ttaataatgg gagcttattc
     1021 gcatcaactc gcttacctga aataatttca ggttttgcaa aatgatagcc ttgaaacata
     1081 tcgaaaccga gagccgtaca tttctttgcc aactcagggc tatcaacctt ctctgctaac
     1141 aagataactg gccagcgtct taatgtattg ataatttctt ttagcttggt ttcatcaatt
     1201 gcaagcacat cgagttttac aacattgaca ataggcaaca tctgcaatgt tctactattg
     1261 atctcagtta catcatcaag ggcaaggtgg taacctattt gctttaactc taaacagcgt
     1321 ttaactactt gatcggtaat ttctattgat tcaagtaatt cgagtacgat atgattactt
     1381 ggcagtaaca gtatcgcgtc gctcatcagt aattctgtac taatgttaat gaagcctcgc
     1441 tgttgaccaa gtactcgctg tatgcctagt tgaccgtaag cattaaccat aacactggct
     1501 gatgcatgaa catcattggt aacgttagct tcgttgattt ctccagcacg gaataataat
     1561 tcaaatgcca ctaaattctg gttacgatcc aatatgggtt gcctacctaa aaatacttcc
     1621 tccattacgc tactccagaa ttgaacgtca tgtataaact agaaaaaata gctacaatta
     1681 tttgtgattg tggctaaaag taaaataata aattacgcaa ttatttcaat acgattttta
     1741 ccgtactgtt tagcacggta aagtgcttta tcggcacgtt taatcacagt attttgtgtt
     1801 tcctgaaact tacgcagtgt aatgccaccg ctaaacgtaa ttttaatcgg tgtttcttca
     1861 gtatctaaca aagggttctt tgataaatta ttttgcaagc gctgcataac ccaaatcgct
     1921 tcttcaggtc tcgtatcagg caatatgatc aggaactctt ctccgccata gcgtgcaatt
     1981 acatcacttg ggcgtatcgt ttttttagct atagttgcga aatgaatgag tgcactgtct
     2041 ccaaataaat ggccgtgagt atcattaatg aatttaaaat catccagatc aattaaaaca
     2101 acacagagcg cattttcata acgatctgcg cgagcagctt cacgcataaa aatattgtcg
     2161 aggccccggc gattataagt accagtcatt tgatctgtat gaacgagctc cctgagctcc
     2221 tctaattcat attttaaaga ctcgatctta ttttctgcat ggcgaagctc gcgggtgttc
     2281 tgtatttcat tattattatg taatcctttt gtttcattaa ggagtgtatc taagatcttg
     2341 atcatttcat caacatcatt agttttacgt attctttgcg caaactgctc aacttgctgg
     2401 taatattcat tcgctgcagt taaagaattg ctaccccctt cattctcagg cttgcgcata
     2461 cctactccag atgtattgat taatcttagc aatttttctt tttttaagct cttcatatcg
     2521 atgagacctc ttagctggct tcataaatga aatttccact ctagataagt atggattgtt
     2581 ttttgaagga gaacttacct agaatttatt tcttcgttta gcagattagc aaatctatgt
     2641 tgaagtaaga aatgaaacta aggatgaaaa gaccctttat tctgtttttg ctttaacaat
     2701 ggttttagtc aactaattta tgtttaattg cataacgaac taattcagaa ttattggaaa
     2761 tttttaattt ttccatcatt cttgcgcgat aaacactgac cgtttttgga cttaaacaca
     2821 tttcaataga gatctcagag agtgatttac ctgatgcaat caagcataaa gtctgaaatt
     2881 cacggtcaga aagattttca tgaagtggtc gatccgcttc tgaagtccat gcatttgcta
     2941 attcttgtgc caatgcagga gagatatatt tatgtccttc agaaacttgt ctaattgctg
     3001 ttacaagttg cgctggcgca ctttgcttat ttaaataccc ggaagcgcca gccttaagcg
     3061 cacgtaatgc aaattcattc tcattatgca tactaagcat aagcaccatt aaattgggtc
     3121 ggtctttctt aatttgcttt aatatttcga taccattccg atcaggtaaa gaaatatcta
     3181 acagcatcac gtcaaactcc tgctggcgta tgacttttac cgcttggaac ccggtttctg
     3241 cttcccctgt tacgctaatg tcccccgttt cacttaaaat ttgttttaag ccttgacgta
     3301 cgatcgcatg atcgtctgca atcattactc gaatcatctt tatcctttgc gactattgag
     3361 tatcaagggt taactaattt tcttggtttc tataggaatc ttaatcagcg cactagttcc
     3421 ctttccttta gtacccgtta tacaaaaatc accgcccaat tgttggcaac gttcacgaat
     3481 tccgcgaata ccaaatgagt ttggcttttt catttcgagg tcagtaatac ctttcccatt
     3541 atcaataact tctaataaaa catgattatt tatctcagca agtttaactt taacaataga
     3601 tgcatttgca tgttttgtaa tattcgtcag agtttcctgg aatattctaa agattgctac
     3661 ggccagatca tcgttgagtg aaatatcttc actctcacat ttcacctcac aaggaatatg
     3721 attacgttta ctaaactccc ttgcttgcca gtaaaccgat gcaacaatgc caacatctag
     3781 aacgcctggc ctaagatcta atgagattct acgagtacta tcaattacat agtcaaccaa
     3841 ctcttcaatt gatctagctt tctgttgata aaattctggt aacctaggtt tatcgatatc
     3901 aatgcacgat tggagctcac atttgattgc tgttaatgtt ccgccaatgt catcatgaat
     3961 ttcacgagca atatgcatac gctcatgttc tttaattttt tgtaaatagg aagataattc
     4021 tgctagctgt gcatgtgaat ttgcaagttc aatttctgct aatttattac gagtaatatt
     4081 gagcacaatt ccgtcccata gaatgcctcc ttgattagat tgccgaggag ttgctcgcaa
     4141 actaatccac tttatatcac tatccccttt tactctaatc tttccttccc aattccaggt
     4201 agaaaaattt ttagctgatg ctttcatttg ctcattatat gaatactggt cttcaggtag
     4261 tatcatttga taaaacaaat caggttgtac ggtcaactcc tcagctgtta atcctagtag
     4321 catcagcacc ccttcactta caaatggaaa agcaaccgat ttgtcgggtt tttttaagaa
     4381 ttgaaatacc aaacctggca aattggacgc aatcgcgcgg aatcgtgctt cactcttttg
     4441 aaggctaatc tgagtatcac gattttcttg aatacttttt agttcttgag cgcacttttt
     4501 aataactgga gcaagcctaa caagatttga aatcagcata aaatcatatg ccccagcttt
     4561 cagaaaatct ttagctgatt gctcattact ttcattcgat ataataatta ctggaattct
     4621 caaatattga ttgacttgag aaaaataagt gagtattgca tgagtacctg tgggcattgt
     4681 agcttcattt gctatgatga tatcccagtg acacttttgt atttcatgta agaactcatc
     4741 ataagtgccc accaatttag aatcaaaatt aatatcctca tccgtgaata tcttacgaat
     4801 agtctgcgca ctctgattta gaaaacctac taagagtata gatagagact cactcacaat
     4861 aagtatttgt taattaatga attgcaatca gcataaccga ccattatcac catcgcaagt
     4921 ataataattc atattcattt gttataaata tatataagtc gcctatgact aattaaactt
     4981 tttattagtc attccgttta ccttcatcaa cagcgactca tcatgacctg aatagattgc
     5041 aggtagagtc aacgtcaccg tgagcggcac gagcgccgca tttttactga tctatagtaa
     5101 tcaaactctg aaggagaaac aaatgccaca aattatcaat actaatattg cctcgttgaa
     5161 tgctcaacga aatttaaatg gttcacaagg gatgctgaat actgcgttag agcgtttatc
     5221 atctggctta cgtattaaca gtgctaaaga tgatgcagct ggtctcgcaa tttctgaaag
     5281 aatgagcacc caaattagag gccttaatca ggctgcgcgt aacgcaaatg atggcatatc
     5341 actttctcag acagctgaag gtgcattaac tgaaattggt aataacttac agcgtattcg
     5401 tgaattgtca gtgcaagcag ccaatgcgac taataccgca agtgatcgtg cagcactcca
     5461 ggctgaggta acacagctaa cttcagaaat tcaacgtgta gctagtcaaa ctcaatttaa
     5521 cgggttgaac ctgttagatg ggtcattcag cacaatgaat ttccaagtcg gtgcaaatgc
     5581 aaatcaaacg atttcagctt cctcaggtaa tttccaaact aatgcatttg gtaattatcg
     5641 tattggtggc ctagctgctt tcactcctgg cggatccggt gatttagtgg caggaacaat
     5701 cggaacaaat agcgatgcct tcggtaactc cgtactttta agtggaacaa caggcgatac
     5761 atccggcatt aatggtgctc ttgcagcggg agacttcaga atctctactg gttctggtaa
     5821 tttcgacgtt tattatcgtg ctggtgcaag cgcaaatgaa atcgcaaccg caattaaccg
     5881 taccggtagc ggtgtaaatg caagtgcaac gactgaagtc gtattaggtg cagatactgg
     5941 cgctggtgct ggtttccaac aaaatacaac ctactcgttc gcaatatcaa ccgattattc
     6001 cgatccaacg aatgcaaata cagtagaccc gaaatatacg accgtttcat ttaaaaccgg
     6061 tggtgcggat gatacagcag atgttgattc tggtagttat ctgagtactg ccgtgcaagc
     6121 ctttaatgat gcatctgcta aaacaggttt tacagcagaa gcagtattaa ccgaggatgg
     6181 ttattgggcg cttaagctga caaattcgca aggtaatgat cttcgtctct tgaataattc
     6241 atacgataat accggaactg ctttggatat tacggtatca gatactgcgg tattagatgg
     6301 cgatacaggt actgctgata gtttagctga tacattagct ggtggcggtc ttgatgcgac
     6361 cactggagtg tggacaaatg gaaatggcgc ttggtatacc gggaaagtaa cgcttgattc
     6421 tgcaaagtca tttactgtaa ccactgctgt tgctgatgtt tttcaagacg ctggaacacc
     6481 tggcactgct gcagcaggca cctatggtgc tcaattgcaa gcggctagcg cgatggatat
     6541 tacgactgcg gaagcagcta ctcgtacact cgcaattgtt gactctgcct tgacaacagt
     6601 taatgatcaa cgtgcgcgct ttggtgcgat tcaaaatcgt cttgaaagca cgattagcaa
     6661 tctgcaaact tcttctgaga atttgtctgc ctcacgcagt cgtatccgtg atgcggattt
     6721 tgcagcagaa actgctagca tgacaagagc acaaattcta caacaagctg gtgttgcaat
     6781 gctttctcaa gcaaatgcaa tacctaacaa tgtattgtct ttattaagat aagcaatagt
     6841 attgataatg cttggtttag ggtagcagtg atcaatgaac tatcctaaac cagttataga
     6901 gaggagttta aaatgatgat aagttcaact acgggaatgg cagatttttt gcaacccgta
     6961 ttacctcagc agaaggtaca gaaaagtgag catatgcctg ctcagactac ggtcactcct
     7021 gaatcaaatc cagtaaataa acaaaatacg attgaattgg atgtaaaaga agcagtaaac
     7081 caaatccagc attttacaga aacgctagct ccaaatttgc agttctcgat tgacgaagaa
     7141 actggaaaaa ctgtagttaa gatcatggat gctcaaactc aggaagtaat acgccaattt
     7201 ccttctgagg aagcgatttc gattgcacgt gccttaggaa aaattgaagg gctattgttc
     7261 catgataagg cataaatgta actaatagtt gtataaagga aattaaaatg atttcttcac
     7321 caggacttgg ttcaggatta gatgtcaatg gaattgttgg gcaacttatg gcaattgaac
     7381 agcgcccact tttactactt accaataaag aagctactca gcaagcacaa atttctgcct
     7441 atggaagctt aaaaggagct ttatcttctt tccagagtag tgttaagaca ttaaccaatc
     7501 tatcgatatt tactggtatt aaggctaatg taggagattc aacaatagct actgcaagtg
     7561 catctactaa tgcagcagta ggtagttatc aaatagaagt tcagaacctt gctcaggccc
     7621 agaaaatcaa atctgaatca ttcgcaacta ctagtacagc aataggaagt ggcgcactga
     7681 caattgagtt tggtacttat aatggtgatg gaacgtttac tagcaatcct acaaaaatat
     7741 ccaaaactat aacaatcgaa tctgatcaaa cagacttggc aagtattcgc gactcgatta
     7801 atgaagcaga tgctggaatt acggcaagca ttgtcaatga tggaagtggg aatcgtttag
     7861 tattcgcatc aaataacacc ggtacagata atgcgctaaa aattacagtg agtgatgatg
     7921 atggtaataa tctagataat gcaggattat ctaagttagc ttacgatgca actacgggcg
     7981 gtactgcaaa tatgacacaa acagtagctg cacaaaatgc aacgatggtg atagatggaa
     8041 taactatcag caagtcttca aatactataa aagacgcctt agagggggta acttttaatc
     8101 tgctaaaagc tgctcctggt actactacaa gcttgtcaat aagcgaggat acatcaaaaa
     8161 tccaaactgc tgttagtgca tttgttggcg cttataatga gcttgtaaaa acaattagtg
     8221 atatctctcg ctatgacaca gctaataagc aagcatctgt tctaacaggg gatgcgacag
     8281 ttcgtacggt tgaaagccaa attcgtgggg tgttgaattc attcttacca ggcgccccaa
     8341 gtagcttaaa ttctctttca cagattggga taaatttcca gaaagatggc acattgcagt
     8401 tagataatgc caagttgact tcggtaatca atgattctga aaaagattta ggctcattat
     8461 ttgctactaa cggattcgca gttaagcttg ataaattagt tgatggcatg cttaagaaca
     8521 atggcttaat tgacggtcgc ctagatggaa tcagtgatac gattaaggat attggcaaac
     8581 agcgagaaat gctaggccgc cgcttggaag atgttgagaa aagataccgc acacaattta
     8641 cggcattgga tactatgatc gcaagtatga ctcaaacaag cactttcctt cagcagcaat
     8701 tatcaaatct accaaaggca agttccggta attagtactt tgatagataa gtaaacttta
     8761 ttaactatat ataagataaa tctatgtctg caaaaaatta tgccattaat tcatatcagc
     8821 gtgttggtgt agagtctgga gtagcatccg ctgattcaca taaactcatt ctaatgttgt
     8881 ttgaagggtc tatcaacgct ctcaatagag ctaaacagga aattctgcac aataatattt
     8941 cagcaaaggg ggagatgatt tcaaaagcca tatcgattat cgataacggc ttaaaagcaa
     9001 gtctcgatat ttctgttggt ggtgagattg caaacaatct gcaggctcta tatgactata
     9061 tgactaggca gcttcttatt gcaaatattc aaaatagtac cggaactatt aatgaagtta
     9121 ttgagctatt gtcccaactt caggaagcat ggcggtctat aggaaataaa gaacttgcta
     9181 aaccaaatat tcatcaaatt agtaatactc acgctttaga accaacagat atttaaaaaa
     9241 tttatggatg gagtacaagt aatttttact tacaaagcaa tactaactgt tactgaaaaa
     9301 atgctaaaag ctgcacaagc aaataattgg gaagaattaa tatctcttga gcaagaatgt
     9361 aagttattaa cacaagctct tgtatctaat tctaatcaga ttcagctcag tgatgaaatg
     9421 cagaaaaaaa aaatagatat tattcataga atattagata aggatgcaaa aatccgaact
     9481 attactgagc catggatgac gcatctacag aatttactga taaatgcaaa tcagaaacgt
     9541 aaactggatc ggctgtatag ctctgattga gatgtgatgg aaacgacttc ggcagactga
     9601 aataattagt cggtcctgca aaattaactt aaccgtggcg gaggttttgt atacaagaaa
//
LOCUS       DAOQLJ010000087         9574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_2779_length_9574_cov_5.792205, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000087 DAOQLJ010000000
VERSION     DAOQLJ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2779_length_9574_cov_5.792205"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /gene="purH"
                     /locus_tag="PK522_07215"
     CDS             <1..614
                     /gene="purH"
                     /locus_tag="PK522_07215"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393318.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNC41560.1"
                     /translation="RVEAALAEAVIKQFVEVIIAPAFSEEAQAVLKQKANVRVLVVPI
                     QAESNRYELKRVGGGLLVQTPDDFHVGEADLKVVTQLKPTPEQIQDCLFAWRVAKFVK
                     SNAIVFCAQGRTLGIGAGQMSRVDSARIASIKAEQAGLGLNGSTVASDAFFPFRDGLD
                     VVVKAGAKIVIQPGGSIRDDEVIQAADEQGVIMLFTGIRHFRH"
     gene            703..2019
                     /gene="purD"
                     /locus_tag="PK522_07220"
     CDS             703..2019
                     /gene="purD"
                     /locus_tag="PK522_07220"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNC41561.1"
                     /translation="MKLLVVGSGGREHAIAWKLSQSPRVQKVFVAPGNAGTALEAGLE
                     NIPITAIAELVAFVKQENIALTVVGPEAPLAEGIVDIFRAEGLRIFGPTQKAAQLEAS
                     KIFAKAFMQCYGIPTANSASFNEADAAHRYINQGAVPIVIKADGLAAGKGVVVAQTHE
                     QAHEAVDRMLTDSQLGKAGAQILIEDFLEGEEASFIVLADGQHALPLATSQDHKRLLD
                     NDLGPNTGGMGAYSPAPCITPELHARIMREVINPTLLGMTQENEPYTGFLYAGLMISP
                     QGDIKVLEFNCRLGDPETQPIMMRLKTDLLMLIEQALNQSLDKTELEWDRRTALGIVL
                     AAEGYPESPRKHDKIEGLVEIMKEQATTKDFHVFHAGTVLNNESPPAILTAGGRVLCV
                     AALGENIKLAHQRAYDIAHKIQFSGCQMRHDIGYRAMNRIDPKVDS"
     gene            complement(2054..2602)
                     /locus_tag="PK522_07225"
     CDS             complement(2054..2602)
                     /locus_tag="PK522_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HNC41562.1"
                     /translation="MKLLFDLFPVILFFIAYKLVDIYAATVAAIIATLIQVGWSWFRH
                     RKVDNLLWVSLVLITIFGGATLFLQDEAFIKWKPTVLYWLFACVLLISQVLFKKNLIR
                     SMLSNQLKLPAAIWSRLNTSWSLFFAAMGAVNLYVAFSFSTDFWVNFKLFGFTGLMLV
                     FIIFQGLFIGKHAEAQDVTKTE"
     assembly_gap    2896..2995
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2996..3183
                     /locus_tag="PK522_07230"
     CDS             <2996..3183
                     /locus_tag="PK522_07230"
                     /inference="COORDINATES: protein motif:HMM:NF016828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HNC41563.1"
                     /translation="NKLVKSFKQFLNNEEGMGAVEYALIVAVMSVIILAVWGTLQGGL
                     QTAFTNISNTLQNPSVN"
     gene            3348..3536
                     /locus_tag="PK522_07235"
     CDS             3348..3536
                     /locus_tag="PK522_07235"
                     /inference="COORDINATES: protein motif:HMM:NF016828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HNC41564.1"
                     /translation="MNKLVKSFKQFLNNEEGMGAVEYALIVAVMSVIILAVWGTLQGG
                     LQTAFTNISNTLQNPSVN"
     gene            3632..4171
                     /locus_tag="PK522_07240"
     CDS             3632..4171
                     /locus_tag="PK522_07240"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNC41565.1"
                     /translation="MDQLNILVLAGLLLVAVYYDIRSFRIPNKLILAGLITGLLFNVI
                     LPHETGGLSFQSALAGFGVGLILFLPLYLIRAMGAGDIKLMGMIGIFVGPLPILLIAL
                     YTAIAGGILAIIVLLCNGKLNGLVEFFKILLLKFQLSTIAGNSVTISIEQMLPKSTSK
                     LPYGVAIAAGTFFYLMTNF"
     gene            4497..5303
                     /gene="cpaB"
                     /locus_tag="PK522_07245"
     CDS             4497..5303
                     /gene="cpaB"
                     /locus_tag="PK522_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HNC41566.1"
                     /translation="MRNTGALGMIVVSLLLGSAAVVVAAKWLNNQVNIEASKIIVASQ
                     DINVGTRLTPEILKAIDWPSSSIPEGSFQEAKLLDSRVAKVNLQRGEPVLEAKLAPEG
                     TVGGLSGVIADGKRAITVRVNDVVGVAGFALPGNLVDILVNIKDDNKNPISKIVLEQI
                     LVLAIAQDLGRDETKPKVVNAVTLEVTPEQAERLDLARSVGSLSLVLRNQIDKSIGST
                     DGVRTQDLLGTKIVAAEPQKEVEKVVVYKSIAPRPTVEIIRGVQKSSANQ"
     gene            5315..6901
                     /locus_tag="PK522_07250"
     CDS             5315..6901
                     /locus_tag="PK522_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HNC41567.1"
                     /translation="MASKRLTYFGLLAVFFCGLQGTAQAHNVASVATTDRINDSHEMA
                     SSMNVTLGKSTLLRLPSAISRISVGNPSVADVTLTSANEVYILGKMIGTTNIILWPRN
                     GSTRVVDITVLMDAAALQDKLLEIMPEETDIHVKAAGDSLVLSGTVSDTVKVDRAVAL
                     ADTYVRTSVLGMMMSMQGNVGQGAAGQGGQGGMQGMQILRQGLQPNEDSAGAGAGLGS
                     IKIVNLLRVRDNQQVMLEVKVAEINRTLAEKLGFDFTRAANKAGSAWTQIMAGVAGGG
                     AFNLLMGKNLGATVNPNLNRITDFSASEGTAILMDAEKKDNLVKVLAEPNIVAISGQE
                     ASFLVGGEIMIPIPSTNQVRLLPKEFGIGVRFTPTVLEAGRIHLRVSPEVSELVSITN
                     VASTGLGQVLAIPTFTTRRISTTVQLNDGQSLAIGGLLQDDFKEQVKRFPMLGEVPVL
                     GALFRSSDFQMKKTELMVIVTPRLVKPLNHDYQLPTDSFVAPDRGEFLIQGRMESASE
                     PTVPPRKNYLQYDQGGFQMR"
     gene            6912..7229
                     /locus_tag="PK522_07255"
     CDS             6912..7229
                     /locus_tag="PK522_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HNC41568.1"
                     /translation="MKNIRFNINYFGCSLAVLTLMACAPVTPRLDARFGDAVGFARAQ
                     QTANPQASFNPTAPEGINGQAGDAIFDNYRDGFRNPQPPLRGVIDVGTSGGGGGRGAQ
                     STR"
     gene            7275..8567
                     /locus_tag="PK522_07260"
     CDS             7275..8567
                     /locus_tag="PK522_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HNC41569.1"
                     /translation="MSQLLTKMLGVPKALPALKKQSGAVAVIIALSLAGLIGFVGLAL
                     DLGKLFVAKTELQNSVDACALAAARELTGANSNQLILAEAAGIATGERHRVYFQGEAI
                     SISSVAFAVSLVDDYRSASDIDPAEAVEMRYARCEANRTDILNWFIQVLNLFPGVDIG
                     SQTVAATAVATLSPSQIISCAIPVGLCTDDVPPSTPVGTWLESVLGPPAGGKKSESGN
                     LTGNFMWVDYSPPGGGASELAGQLTGPGVCSLPAQGSQVGEAGVKSSLANDWNSRFGI
                     NQGSVKEGDAIPDYSGYAYTEVNWPSKFNALSDFQSKRGANIPYQGDASTGLNTHGTA
                     VDATYLQENGADRRLVTVAVVDCDGFTSGTTAPVQAWACVLMLHPINNSQGGSGTGAT
                     RMYLEYLGRSNDTGSPCATNGVPAGPTGIGPMVPALVR"
     gene            8573..9067
                     /locus_tag="PK522_07265"
     CDS             8573..9067
                     /locus_tag="PK522_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HNC41570.1"
                     /translation="MKLMNIRTSLQCGTNKRSVQGAVAVEMALLLALMLVLILGVAEF
                     GRALYQYNTLAKAVRDAARHLSQFNPNDTSDYDEAKEDATCLAVYGNLSCGGEALAAN
                     LTTDMISINTITETSSAGVPITLVEVTITGFTFNFTLSPLVFLGGGDQSITFGDIRAI
                     MRQS"
     gene            9064..9516
                     /locus_tag="PK522_07270"
     CDS             9064..9516
                     /locus_tag="PK522_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNC41571.1"
                     /translation="MKCISNYTFGPKLIIRSYQQGMVAVEFALITIILVTFLFGAMEM
                     SRALFYWNTATEATRLGARLAVVCDQDASAIKTKMQAMLGVLEADQISVTYLPDGCDS
                     NSCRSVTVSINGFNFAMLVPVLPLNIPMPPFSTTLPRESMDSENPDCT"
BASE COUNT         2655 a         1859 c         2352 g         2608 t
ORIGIN      
        1 agcgtgttga agctgcttta gccgaagcgg ttattaaaca attcgtggaa gtcattattg
       61 cccctgcgtt tagcgaggaa gcgcaagccg tactcaagca aaaagcgaac gttcgtgtgc
      121 tggtcgtgcc cattcaggca gaatcgaatc gctacgagct gaaacgcgtt ggaggcgggt
      181 tattagttca aacacccgat gattttcatg tcggggaggc tgatctcaaa gttgtgacgc
      241 aattgaaacc aacacctgaa cagattcaag attgcttatt tgcttggcgg gttgcgaaat
      301 ttgtgaaatc aaacgccatt gtattttgtg cacaaggtcg gacgctgggt attggtgcag
      361 gtcaaatgag tcgggtcgat agtgctcgca ttgcatccat caaagcggag caggcgggtt
      421 taggtttgaa tggctcaacc gttgcatccg atgcattttt tccatttcga gacggtttgg
      481 atgtcgtggt taaagcaggg gctaagattg tcatacaacc cggtggtagc attcgtgatg
      541 atgaagtgat tcaagcagca gatgagcaag gggttattat gctttttaca ggaattcgcc
      601 attttaggca ttaaaataac cgaaacgtcg cgataataga ttactttttg cttaatcggt
      661 cattacatta actattcttt tctaagctgg tagtgtataa caatgaagtt acttgtagtc
      721 ggtagtggag gtagagagca cgcaatcgcg tggaagctga gtcaatcgcc acgggttcag
      781 aaagtgtttg ttgcgcccgg taatgcaggt acagcgcttg aagcggggct cgaaaatatt
      841 ccaattacag ccattgctga gctagtggca tttgtaaagc aggaaaatat tgccctaacc
      901 gtagtaggtc ctgaagcgcc cttggctgaa ggaattgtcg atatcttccg agcggaaggg
      961 ctccgtattt tcggtccaac ccaaaaagcg gcacaacttg aagcttcaaa aatctttgca
     1021 aaagctttta tgcagtgcta tggcatcccc actgcgaatt cagcaagttt taatgaggct
     1081 gatgcggcac atcgttatat taatcaaggt gcagtaccga tcgtgatcaa agcagacggg
     1141 ttagctgcag ggaaaggcgt cgtcgtcgca cagactcatg agcaagcgca tgaggccgtt
     1201 gatcgcatgt taacggatag tcaactggga aaagctggcg cgcagatttt aattgaagat
     1261 ttcctagagg gtgaagaagc gagttttatt gtcctggcag atggtcagca tgcgttaccg
     1321 ctagcgacga gccaggatca taaacggctt ttagacaatg atttaggccc taatactgga
     1381 gggatgggtg cttactctcc tgccccctgt attacccccg aactacatgc gaggattatg
     1441 cgagaggtta tcaatcccac actattgggg atgacacaag aaaatgaacc ctatacggga
     1501 ttcttatatg ccggcctgat gatatcacct caaggtgata tcaaggtgct ggaattcaac
     1561 tgccgtttgg gtgatcctga aacgcaaccg attatgatgc ggctcaaaac ggatttattg
     1621 atgttaatcg agcaagcgct aaatcaatcg ctggataaaa ctgaactcga gtgggatcga
     1681 aggaccgctt taggtattgt gttagccgct gagggttatc cagaatcacc acggaagcat
     1741 gacaagattg aaggtttagt ggaaatcatg aaggaacaag caaccacgaa agattttcat
     1801 gtatttcatg ctggtactgt gctaaataat gaaagcccgc ctgcaattct tacggcaggg
     1861 gggagagtat tatgtgttgc tgcacttggt gaaaatatta aattggcgca tcaacgtgct
     1921 tatgacattg ctcacaaaat tcaatttagc ggctgtcaaa tgcgccatga tatcggttat
     1981 cgcgcgatga atcgaatcga tcctaaagtc gacagctgat ttcgtctcat tgaatcgatt
     2041 acccaagcgc aactcattct gtttttgtta catcctgcgc ttccgcatgt tttccgataa
     2101 acaacccttg gaaaataatg aaaacaagca ttagccctgt aaatccaaag agcttgaaat
     2161 tgacccaaaa atcagtcgag aagctaaatg caacataaag gttaaccgct cccatcgcgg
     2221 caaaaaatag tgaccaactg gtattcagac gcgaccaaat ggctgctggt aatttgagtt
     2281 gattgctgag cattgaacgg atcagatttt tcttaaaaag cacctggctg atcaatagca
     2341 cacaagcaaa taaccaatag agcaccgtcg gtttccattt gataaaagct tcatcctgaa
     2401 ggaatagcgt tgcaccccca aaaatagtga tgagaaccaa actcacccat agcaaattat
     2461 ccactttgcg gtgacgaaac catgaccaac ccacctgtat aagcgtcgca ataatcgctg
     2521 ctaccgttgc agcgtagata tcaaccagct tataagcgat aaaaaataat atgactggaa
     2581 ataggtcgaa aagtaatttc atgttgagtg ttgtaagtga ggttacgcga taaacgaagg
     2641 ctgaaataac agggcctagc tttaaaaaaa tactcatcat acctgatgcg ttactaaagt
     2701 tcacttacag gccattgctg cgattaatgc atccctccat ttttagtaat actacttttt
     2761 ttgatttctt atatcgctat ctgaagctgt tgctgtacgg aagtacgatg taccagggta
     2821 cgataggcga agcggcggta atttgtcaga atttcaccat gcagtaacga ttgaagaagt
     2881 taaacaaatt aaacannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgaac
     3001 aaattagtta aatcattcaa acaatttttg aacaacgaag aagggatggg ggcagttgag
     3061 tatgctttga tcgttgcagt aatgtcagtg attattctgg cggtttgggg aacgctacaa
     3121 ggtgggttac aaactgcgtt tactaatatt tcaaatacat tacaaaatcc tagtgtcaac
     3181 taaattactt taattatctg gtgtgtataa gtagcaattt tgaacggtta gacctgtacg
     3241 gaagtgcgat gtaccagggt acgataggcg aagtggcggt gatttgtcag aatttcacca
     3301 tgcagtaacg attgaagaag ttaaacaaat taaacaggag aaaagacatg aacaaattag
     3361 ttaaatcatt caaacaattt ttgaacaacg aagaagggat gggagcagtc gagtatgctt
     3421 tgatcgttgc agtaatgtca gtgattattc tagcagtttg gggaacgcta caaggtgggt
     3481 tacaaactgc atttactaat atttcaaata cattacaaaa tcctagtgtc aactaaacta
     3541 ttttaattta tctcagtagt aagtaagaag taattccata aaattatcca cccagatata
     3601 actgggtgga tttctaaatt aagatatcga tatggatcaa ttgaacattc tagtacttgc
     3661 agggttatta ctcgttgcag tttattatga tatccgtagt tttcgaatac ctaacaagct
     3721 gattcttgct ggactgatta ctggattgct ttttaacgtt attctgccac acgaaaccgg
     3781 tggattaagt tttcaatcag cgttagctgg atttggagtt ggactaatcc tttttttgcc
     3841 gctttatctc attcgtgcga tgggtgcagg tgatatcaaa ctaatgggta tgattggaat
     3901 ttttgtaggc ccactaccga tattgcttat cgcgctgtat accgcaatag caggtggaat
     3961 acttgcaatc attgttttgc tttgcaatgg taaattaaat ggactagtcg aattttttaa
     4021 gattttattg cttaaatttc agctaagtac aatagctgga aattcggtaa caatttctat
     4081 tgagcaaatg cttcctaagt ctactagtaa attaccttac ggagtagcta ttgctgccgg
     4141 gacattcttt tacctaatga caaattttta attggcgaat ctgagataaa ctaataaacc
     4201 gcgtttaatc tcgtaatttt cttcttttct aacaccatca attcagagaa tagtcaaata
     4261 atcgtgcact tatttgactt caactcgttc attcattagt cgaagtaact gtctattgca
     4321 actgcagccg aatgtcatgt cgatgcgttg catcggtcaa gatagggtca gtttattgat
     4381 cttttccatt tattgcgtga gagcgataac cactatagtt acccttgaga accatggcaa
     4441 taaacctaca ggcattactt aattcataac tcgttaacta ggtgtgaggt ctatcgatga
     4501 gaaatacagg agcgctagga atgatcgtag tctccttgtt attaggatca gcggcagtgg
     4561 tcgttgccgc gaagtggctt aataatcagg tgaatatcga agcatcgaaa ataatcgttg
     4621 cgtcacaaga cattaatgta ggcacccgcc ttaccccaga aattctcaaa gccatcgatt
     4681 ggcccagcag cagcatccct gaaggctcat ttcaagaagc aaaactcctg gatagtcgcg
     4741 tggcaaaagt gaatttgcaa cgtggtgaac cggttctaga agctaaatta gcaccggaag
     4801 ggacggtggg gggattatcc ggtgtgatcg cagatggtaa gcgtgccatt accgtgcgcg
     4861 tgaatgatgt cgtgggcgtg gctggttttg cgcttcccgg taacttggtc gatatcttgg
     4921 taaatattaa agatgacaac aaaaatccaa tttctaaaat cgtactcgag cagatattag
     4981 tgttggcgat tgctcaggat ttagggagag acgaaacaaa accaaaagtt gtcaatgcgg
     5041 taaccctaga agtaactcca gagcaagcgg aaagactcga tctggcaagg agtgttggat
     5101 cactttcttt ggtgttaaga aatcaaatcg ataaaagtat tggttctacc gatggtgttc
     5161 gcactcagga tttactgggt acaaaaattg ttgctgcaga acctcaaaaa gaagttgaga
     5221 aagttgtcgt gtacaaatcc atagccccca ggccaacggt tgagatcatt cggggtgttc
     5281 aaaaatcgag tgcaaatcaa taaaggggag aatcatggcg tctaaacgat taacttattt
     5341 cgggttatta gcggtatttt tttgtggatt gcaaggaaca gcacaagcac acaatgtggc
     5401 atcggttgca accacggatc gaatcaatga tagtcacgaa atggcttcca gcatgaatgt
     5461 gacactgggt aaatcaacct tgctgcgact tccttccgca atttccagga tatcggtcgg
     5521 taatccgagt gtggcagatg taacgttaac cagtgccaac gaagtttata ttctcggaaa
     5581 aatgatcggc accacgaata ttattttgtg gccgcgcaat ggttcgactc gggtcgtgga
     5641 tattaccgta ctaatggatg ccgctgcact tcaagataag ttactagaga tcatgccaga
     5701 agaaacggat attcatgtta aagcggccgg tgattcatta gtactctctg gaacggtttc
     5761 cgatacggtc aaggtagatc gtgcggtagc attagccgat acctatgtcc gcacttcagt
     5821 actgggcatg atgatgagca tgcaagggaa tgttggccaa ggcgctgcag gtcagggggg
     5881 gcaaggtggt atgcaaggca tgcaaatctt acggcaggga ttgcaaccca atgaagattc
     5941 agccggggca ggtgcagggt taggtagtat caaaatcgtc aatttgctgc gtgttcgcga
     6001 taatcagcag gtaatgctcg aagtcaaagt ggctgaaatt aaccgcacct tggctgagaa
     6061 acttgggttt gattttacgc gtgctgcgaa caaggcaggc agtgcttgga cacaaataat
     6121 ggctggcgtg gctgggggtg gagcatttaa tctgttgatg ggaaaaaatc taggcgcaac
     6181 ggttaacccc aaccttaatc ggattaccga ttttagtgcc agtgaaggta cggcaatcct
     6241 catggatgct gagaaaaagg ataatttagt taaagtgtta gccgaaccga atatcgtagc
     6301 aatcagtggt caggaagcta gtttcttggt ggggggcgag atcatgatcc cgattcccag
     6361 taccaatcaa gttaggctcc tacctaaaga gtttggaatc ggcgtgcgat ttaccccaac
     6421 ggtgttggaa gcaggtcgaa ttcatttacg ggttagtcca gaagtatccg agttggtcag
     6481 tatcaccaat gtggcctcaa cggggttagg tcaagtgtta gcgattccaa cgtttacaac
     6541 tcgccgtatt tcaaccaccg tgcaattgaa cgatggtcaa tcgctggcaa tcggtgggtt
     6601 gttacaggat gactttaaag aacaagtgaa acgttttccc atgcttggtg aagtgccagt
     6661 tttaggtgca ttgttccgca gtagcgattt ccaaatgaag aaaacggagc tgatggtcat
     6721 tgtaacacca cggctcgtga agccattgaa tcatgattat caactgccta ccgattcttt
     6781 tgtggcaccc gatcggggtg agttcttgat tcaaggtcgg atggaaagtg cttccgaacc
     6841 aacggttccg cctagaaaaa attatttgca gtatgaccaa ggtggctttc aaatgagata
     6901 aggagaaaaa ggtgaagaat attcgattca atatcaacta ctttggttgt tcactcgcag
     6961 tcttgacatt aatggcgtgt gctccagtca cgccaagatt ggatgcacga tttggcgatg
     7021 ccgtcgggtt tgcaagagcg cagcaaacag cgaatccaca agcttctttc aatccaacag
     7081 caccggaagg tatcaatgga caggctggag atgccatctt tgataactat cgcgatgggt
     7141 ttaggaaccc tcaaccacca ttacggggtg tgatcgatgt cggaacttct ggaggcggag
     7201 ggggaagggg tgctcagagt actcgatagc aatggcgcga acgaaatgga actaaatatc
     7261 ggataaatcg ctatatgagc cagttattaa ccaaaatgct tggtgtaccc aaagcgttac
     7321 ccgctttaaa gaaacaaagc ggtgcagttg cagtgattat tgcattatct ttagcgggtt
     7381 taatcggttt tgtgggcttg gcgcttgatc tggggaaact ctttgtggct aaaaccgagc
     7441 tgcagaatag cgttgatgcg tgcgctttag cggcggcacg cgagttaaca ggggccaata
     7501 gtaatcaact tatcctcgct gaagcggcag gaattgcaac tggcgaacgc catcgcgttt
     7561 attttcaagg tgaagctatt tcgatttcca gtgttgcctt tgctgtatcg ctcgtggatg
     7621 attatcgatc cgcttctgat attgatcctg ctgaagccgt tgagatgcgc tatgctcgct
     7681 gtgaagcgaa tcgtaccgat atcttgaatt ggtttattca agtgttgaat ttgtttcctg
     7741 gcgtggatat tggaagccag acggttgctg caactgcggt tgctacccta tctccttccc
     7801 agattatttc ttgtgcaatt cccgttggcc tatgtactga tgatgttcct ccgagtacgc
     7861 cagtcggtac ttggcttgaa agtgtgttag gtcctccggc gggtggaaag aaaagtgagt
     7921 cgggtaattt aactggaaat ttcatgtggg ttgattattc gccacctggt ggcggggcat
     7981 cggaacttgc agggcagtta acgggcccag gggtttgtag cctgcctgcg caaggctccc
     8041 aagtcggtga ggctggggtt aaatcctcat tagcgaatga ttggaacagc cgatttggca
     8101 ttaatcaagg tagtgtcaaa gagggcgatg ctatccccga ttacagtggc tatgcttata
     8161 ctgaagtcaa ttggccctct aaattcaacg ctttatccga ttttcaaagt aaaagaggcg
     8221 caaacatacc ctatcaaggg gacgcgagta cgggacttaa cacccatgga acagcggtcg
     8281 atgcgactta cctacaagaa aatggtgctg atcggcgttt ggtaacggtc gcagtagtcg
     8341 attgtgatgg ttttaccagc ggaacaactg cacctgttca agcttgggcc tgtgtattaa
     8401 tgctgcatcc gattaataat tcacaaggag gcagtggaac cggtgcaacg cgcatgtatc
     8461 ttgagtattt ggggcggtcg aatgataccg gtagcccttg tgcgaccaat ggcgtacccg
     8521 cagggcctac tgggattggc cctatggtgc ctgctttggt gaggtaagcg atatgaaatt
     8581 aatgaatata agaacgtctt tacagtgtgg tactaataaa aggtcggtgc agggtgctgt
     8641 tgctgttgag atggctttat tacttgcctt gatgcttgtc ttgattttag gcgtagctga
     8701 atttggtcga gcgctctatc aatacaacac gctggctaaa gcggtaagag atgctgcgcg
     8761 acatctttcg caattcaatc ctaacgatac cagcgattat gatgaagcga aagaagatgc
     8821 aacttgctta gccgtttatg gcaatttaag ttgcgggggg gaggcgcttg cggcaaattt
     8881 aacgacagat atgatttcta ttaatacgat cactgaaaca tcgagtgctg gtgtgccgat
     8941 cactctagtt gaagtaacta taaccggatt tacctttaat tttaccctta gtccccttgt
     9001 atttctcggt gggggagatc agtcaattac gtttggcgat atacgtgcca tcatgaggca
     9061 atcatgaaat gcatttcaaa ctatacattc ggtcctaaat tgatcattcg atcctatcaa
     9121 caggggatgg tggcggtaga gtttgcgctt atcacgatca ttttggttac gttcttattt
     9181 ggggcaatgg agatgagtcg agcgttattt tattggaaca cagcgacgga agcgacacgc
     9241 ttgggtgcgc gtttggctgt cgtctgtgat caagatgcca gtgcgatcaa gaccaaaatg
     9301 caagcgatgc tgggcgtgct tgaagccgat caaattagcg taacgtactt gcctgatggc
     9361 tgcgatagta attcttgtcg atcggttact gtcagtatca atggatttaa ttttgcaatg
     9421 ctggtacccg tgttaccact gaatatcccg atgccaccct tttccacgac attgccacgg
     9481 gaaagtatgg atagtgaaaa tccggattgt acatagtttt atttgaacaa attatctgaa
     9541 ttaatgggta gaggagtaat acattgaaaa tcgc
//
LOCUS       DAOQLJ010000088         9005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3024_length_9005_cov_6.796648, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000088 DAOQLJ010000000
VERSION     DAOQLJ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9005
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3024_length_9005_cov_6.796648"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PK522_07275"
     CDS             <1..182
                     /locus_tag="PK522_07275"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HNC41572.1"
                     /translation="INHRSALFQSFLKDCADRRIQLSRFINFYNTVKPHKSLNNATPY
                     EILNAYFNQPLCKQL"
     gene            complement(441..527)
                     /locus_tag="PK522_07280"
     tRNA            complement(441..527)
                     /locus_tag="PK522_07280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(491..493),aa:Leu,seq:caa)
     gene            complement(656..1477)
                     /locus_tag="PK522_07285"
     CDS             complement(656..1477)
                     /locus_tag="PK522_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM/exosortase system-associated
                     acyltransferase"
                     /protein_id="HNC41573.1"
                     /translation="MSQNEIINIGATFSEHFEIVPALSEELKNEAFRVRHQVYCEDLN
                     YESQRASKYETDEYDINALHLLMKSVRLNKFIGCTRLIRPSIDDPHQPLPFEKCCGNT
                     LDHSKVGSLLLSRNKIAEVSRLAVISEFRRRKGESERPINLSDEDYGISNYARFRFPY
                     IPLGLFIGTVELAYIHHIDILFMLTEKRLAMHFSKLGANLDFIGMPVEHRGVRVPSMV
                     NTVEIIKNMKPMFRPLYNLISKTICESLSRSTQNPFIIQIGSDPQNTANQQLNRL"
     gene            complement(1535..2308)
                     /locus_tag="PK522_07290"
     CDS             complement(1535..2308)
                     /locus_tag="PK522_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNC41574.1"
                     /translation="MTNMSQQYFMNYLLFLYFTLIPIAVFANTTEDTDTLARAILEKA
                     DAIRFPRESFQVEVDIKTISPEKINEEYKYRVLSKGNENSIVMITEPASERGQTILMK
                     ERDLWVFMPSVSQPIRLSLSQRLVGQVANGDIARANFSGDYNPKLLRTEIINDKAYYV
                     LELTAVDRSVTYHKVVLWVQESNYWPYRAEFYSLSNRLLKTSRYEKFDWVLGIERPTR
                     LIMEDALKKDEVSILDYSEMILRDLPDKIFTKEYLKKLD"
     gene            2360..3061
                     /locus_tag="PK522_07295"
     CDS             2360..3061
                     /locus_tag="PK522_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC41575.1"
                     /translation="MNIVQISNVSKEYMLGEQHVQALKDVNLEIEESVFLAIAGPSGS
                     GKSTLLNLIGCIDNPTTGKIFIDGQDTSGKTPDELSELRLRNIGFIFQTFNLFPVLSA
                     EENVEYPLLQIKELDSDERQERIVKFLDIVGLSKFAHHRPNQLSGGQRQRVAIARALA
                     THPKIILADEPTANLDHNTGEGILELMKEINRKDKTTFIFSTHDSRVMSMADRLIHIE
                     DGRIIRENTVNSMGS"
     gene            3323..4507
                     /locus_tag="PK522_07300"
     CDS             3323..4507
                     /locus_tag="PK522_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41576.1"
                     /translation="MAFAYADPDHLSKMRLRTELENIRQINSFIKWKLSGRIDYDAVY
                     SISNFYSHNVREDQRFEFWLRENYLDISRGNWDFRVGRQHIIWGEMVGLFFADVVSAK
                     DMREFVLQDFNLLRIPQWALRTEYSKNNFHADLVWIPFATLDKIGRPGEPGRSAAEFY
                     PFALPDQLQATFLNEDRSGRNITNSNYGLRLSQLVNGWDFSAFYYHSLDASPTFYLVG
                     GELGRPVFQARHDRIDQVGSTFSKDLGSVVVKGEFVYTNGRQFNVTRFSQPFGLVQQD
                     TVDYALGLDFSLPAEIRTNLQFFQRVFLSYDRDIFHDKWESGASIMLNRKLWQDVEAQ
                     TLLIHSLNRVEWMLRPRINWNFTKNWRLALGADMFEGPATGLFGRFANNDRIFTEIRL
                     SF"
     gene            complement(4508..5728)
                     /locus_tag="PK522_07305"
     CDS             complement(4508..5728)
                     /locus_tag="PK522_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC41577.1"
                     /translation="MLKLSLRNILRQKIHTTMSLISIICGVVGIILAGGWVNDIFIQL
                     GEALIHSQSGHIQIYKQGYFYEGSRSPEKYLIYDFDQMKKTVAQQITVEKFDSITARL
                     NFSGLLNNGRSDLPIVGEGIHPENEGQFSTALKITDGRQLTDQDKFGLMLGRGLANGL
                     KLKPGDTVTLLTNTIEGTLNSLDFEVIGIFQTFSNDYDARTIKISLATAQELLSINAV
                     NALVISLKETKDTDQITALLKNKLDSFDLEVRTWTELNDFYEKTVDLYKSQFGVLQLI
                     ILVMVCLSVANSVNMSIYERTGEFGTMLALGNRSMDVFYLIISENLLLGCIGASLGIA
                     IGMFLALTISAIGIPMPPPPNADLGYIAQIRIVPSVLLLAFLIGLFATVIAAIMPARK
                     IAQLPIVEALRQNY"
     gene            complement(5735..6976)
                     /locus_tag="PK522_07310"
     CDS             complement(5735..6976)
                     /locus_tag="PK522_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNC41578.1"
                     /translation="MNSNWEKSIEISLAFRNIIRQKQRSLISLGAIIFGVCSLILASG
                     FIEWIFIDFRETSIRSQLGHLQITRPGYQEEGKANPYHFLLPDTQPELKLTSSSLEKI
                     KVIVPRLSFSGLISSGESTLSFIGEGVDPNEQNFFGDALQLTTGNYLSGNNTFQIIMG
                     EGLSRNLGVETGDNVTLLVNTASGGINAIEVTISGLFTTVTKSYDDNAIRIPIETAQK
                     LIRVTGAHSWIVLLNHTEDTNQVLSLLHDQLPQSKFEITPWYQLADFYNKTTVLLSKQ
                     VLAIKIIIAIIILLSISNTMSISVIERTGEIGTAMALGIKQTKILYMFLTEGILLGCI
                     GGILGVMIGYLLANIISSVGIPMPPPPGMARGYIGEILVTQSMVLEAISLAIGTTLIA
                     SVYPAWKASKLQIVDALRHNR"
     gene            7142..8743
                     /locus_tag="PK522_07315"
     CDS             7142..8743
                     /locus_tag="PK522_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNC41579.1"
                     /translation="MRLENAILEWKKIIGDSQVFDKETAEVEYGSCTTGVSRAISASI
                     KPLDASHIPNIVKIANKYKVALYPISTGHNWGYGTALPSIDNCVILDLSALNKILEFD
                     PDTGLVTLEPGVTQGQLAEFLTLGNHSYMVPVTGAGPTCSLLGNALERGYGITPITDH
                     FGAVISIEAVLADGSIYQSALSAMGGESIDRAFKWNVGPYLDGIFSQGSFGVVTKMTI
                     ALAHRPESVESFLFGLKNSEQLPDLVRLIQKIITRYPGILGGVNLMNAHRVLAMSIPY
                     PRDKLALNKTIAQETLSELCKANHVMPWTGLGTLYGSRKVLKVVKQEIKAMLSPIASQ
                     LIFVNSGLIKTSIKVINFFPNFIRTKFERKLMMLERSMQLVSGWPNQTALPLCYWLMN
                     DSDVRQEVFLNPARDGCGLIWYSPLVLMKPDSVMQYINMVTSVMQKHGLEPLVTLTSV
                     TNACFDSTVPLLFDRTSSQGQKSAENCYWELLETGKKLGFVPYRFGIHAMSWLTADET
                     PYWQTVRKIKRSLDPDNIISPGRYV"
BASE COUNT         2699 a         1637 c         1831 g         2838 t
ORIGIN      
        1 agatcaatca ccgttcggca ctttttcaga gtttccttaa agactgtgcc gatcggcgca
       61 ttcagctttc ccgtttcatt aacttctaca atactgtcaa accccataag agtttgaata
      121 atgccactcc ttatgaaata ctcaacgctt atttcaatca acctctctgt aaacaactct
      181 gagatttctt acaggttatt ctccgttgat ttcacttttc agcggttctt acctggatga
      241 gacaagataa tcagtttgac ttatgggtga aagcactcat cgaatgctca ttctttggct
      301 ttactggccg agctgcggga tcggtacgca tttgacagta tgtcagaaga caacaagcaa
      361 tcttccggca tagtcaattt taaaaattta atgattgaaa atggaattga aacgatctga
      421 aactacttga atgctgcact tggtgccgag agggggaatc gaacccccat gatgtcacca
      481 tcgcgggatt ttgagtcccg tgcgtctacc aattccgcca tctcggctta tatgtataac
      541 cttaaatcca gcaagtgcac attattactg aaataatagg tctcagcaat cctgttattt
      601 cagtaagtgt atacgttacc tttaatttaa ctactttatg tatcagcttt atcttctata
      661 aacgattgag ttgctgattt gcagtgttct ggggatcaga gcctatttga ataatgaaag
      721 gattttgagt cgatcgcgag agactttcgc aaattgtctt tgagatcaga ttatacaatg
      781 gcctaaacat tggcttcatg tttttaatta tttcgacagt attaaccata gaaggtacgc
      841 gaacaccccg atgttcaact ggcataccaa taaaatcaag atttgcgccc aatttactaa
      901 agtgcattgc taatcttttt tcagttagca taaaaagaat atcaatatgg tgaatataag
      961 caagttctac tgttccaata aaaaggccaa gtggaatgta cggaaagcgg aagcgcgcat
     1021 aatttgaaat accatagtct tcatcagaaa gattgatggg tctttccgat tctcccttac
     1081 gacgacgaaa ttctgaaatt acagcgagcc tggatacttc agcaatttta ttgcgagata
     1141 agagtaatga gccgacttta gagtgatcta aggtatttcc acagcatttc tcaaatggaa
     1201 gtggttgatg cggatcatcg attgatggtc ggattaatct tgtacatcca atgaatttat
     1261 ttaacctaac acttttcatc aagagatgaa gggcgttgat atcatactca tcagtttcat
     1321 atttactagc acgctgggat tcatagttta aatcttcaca atagacctga tggcgtacac
     1381 gaaaagcttc attttttaat tcttcagata atgcgggtac aatttcaaaa tgttcgctaa
     1441 aagttgcgcc aatattaata atttcatttt gtgacataga tcgaagccct ataatagttt
     1501 ttgcgatttg gaagccttgg ggttggcttt aaaattaatc aagctttttt aaatattcct
     1561 ttgtgaatat tttgtccggt agatctctca aaatcatttc ggaataatcg agtattgaaa
     1621 cctcgtcctt ttttagcgca tcctccataa taaggcgagt cgggcgttca atgcccaata
     1681 cccaatcaaa tttttcatag cggctggttt taagaaggcg atttgataat gaatagaatt
     1741 ctgctcgata aggccagtaa ttggattctt gcacccagag aacaactttg tggtaagtaa
     1801 cgctccgatc gacagcggtc aactcaagta cataatatgc tttgtcattg ataatttctg
     1861 tacgtagaag cttggggtta taatctccag agaagtttgc acgagcaata tcaccatttg
     1921 caacttgacc aaccaaacgc tgggaaagcg atagacggat aggttgggaa acacttggca
     1981 tgaataccca caaatcacgt tctttcatta atatagtttg gccacgctca gatgccggct
     2041 cagttatcat gacaatacta ttttcattgc ctttagataa cacacgatat ttatattctt
     2101 catttatttt ttcaggagag atagtcttaa tatcaacttc gacttgaaag ctttctcggg
     2161 gaaatcttat tgcatcagct ttttctaata tggctctggc tagggtatct gtgtcttctg
     2221 ttgtatttgc aaagacagct attggaatta gcgtgaaata aagaaataaa aggtaattca
     2281 taaaatattg ttggctcata ttagtaatct ctttccagta aaatatcgtt tctgtgaaaa
     2341 caacctggaa tcaagtctca tgaatattgt tcaaattagt aacgtatcta aggaatacat
     2401 gttgggtgag caacatgtcc aagcgcttaa ggatgttaat ttagagattg aggaaagtgt
     2461 ttttttagca attgcgggcc catcagggag cggtaagtct actctactta atttaattgg
     2521 ttgcatagat aatccaacga ctggaaaaat ttttatagat ggccaggata caagtggaaa
     2581 aacaccggat gaattgtcag aacttagatt aagaaatatc ggttttattt ttcagacatt
     2641 taacctattt cccgttttat ctgctgaaga gaacgtggaa taccccttac ttcaaattaa
     2701 agaattagat tctgatgaac gacaggaaag gattgtaaaa tttctcgaca tagttggttt
     2761 gtccaaattt gctcatcatc gtcctaatca attgagcggc gggcaacgtc aaagagtagc
     2821 aattgcacgt gctttagcta ctcatcctaa aattatcctg gcagatgagc ctactgctaa
     2881 tcttgatcat aatacaggtg aaggaatctt ggaattaatg aaagaaatta acagaaaaga
     2941 taaaactact tttatttttt caacccatga ttctcgtgtt atgtccatgg cagatcggtt
     3001 aattcatata gaagacggta gaattattag agaaaatact gttaatagca tgggaagtta
     3061 gcaggtgtct tttacgcaga tttataacac gattggaacc tgtttaacaa taacaagcgt
     3121 aatatgcttt gcaagcatct catattgctt tggttctgat aaaacgaata ttctatcaat
     3181 taagcaagag attaaaagcg taggctcaaa tagggttgca tctctagatg atttatttat
     3241 agataataaa acatcttcaa cgaaagtctc taatcaacgt ttcaaacaaa catcaagctg
     3301 gcggggtttc tcacaatatg aaatggcatt tgcgtacgca gatcctgatc acttgtccaa
     3361 aatgcgctta cggactgagc ttgagaacat tcgacaaatc aattcgttca ttaaatggaa
     3421 attaagtggt cggattgatt acgatgcagt ttatagtatc tcaaattttt actcacacaa
     3481 tgtgcgagaa gatcagcgat ttgagttctg gttacgcgaa aattatttag atatttctcg
     3541 aggcaattgg gattttcgtg ttggccgaca acatattatt tggggagaaa tggtcggttt
     3601 attctttgct gatgtggtat cagcaaagga tatgcgggaa tttgtgctac aagattttaa
     3661 cttgcttaga atcccgcagt gggcattgcg gaccgaatat tcaaaaaata actttcacgc
     3721 ggatctggtt tggattcctt ttgcaacgct ggataagatt ggtaggccgg gtgagccagg
     3781 taggtcagca gctgagttct acccttttgc acttcccgat caactacaag caacatttct
     3841 aaacgaagat cgttcgggtc gtaatattac aaattcaaac tatggtttgc gcttatcaca
     3901 actcgttaat ggatgggatt tttcagcgtt ttattatcat agccttgatg catcacctac
     3961 tttctatctt gttggtggtg agcttggtag acctgtattt caggcgcgtc atgatcgaat
     4021 tgatcaagtc ggcagtacat tttctaaaga tttaggctca gtggtggtca aaggtgagtt
     4081 tgtttatacc aacggtaggc aatttaatgt aacgcgattc tcacaaccgt ttgggttagt
     4141 tcaacaagat actgttgatt atgcattagg gctcgatttt agcctgcctg cagagatcag
     4201 aaccaaccta cagttttttc agcgagtttt tcttagctat gaccgggata ttttccatga
     4261 taagtgggaa agtggcgcga gcatcatgct gaatcgaaaa ttatggcagg atgttgaagc
     4321 gcagacgtta ctgattcata gcctgaatcg agtagaatgg atgcttagac ccagaattaa
     4381 ttggaatttc acaaaaaatt ggcgacttgc attgggagct gatatgtttg aaggaccagc
     4441 aacagggctt tttggtcgtt ttgcaaataa tgatcgtatt tttacagaga ttcgtctttc
     4501 attctgatca ataattttga cgaagtgcct caacaatcgg taactgagca atttttcttg
     4561 cgggcataat ggccgcaatc acggtagcaa ataatccgat caaaaaagca agcaatagaa
     4621 cagaaggtac aatcctgatt tgagcaatat aacctagatc ggcattgggt ggaggaggca
     4681 tgggtatgcc gatggcggag atcgttagcg caagaaacat tcctattgca ataccgaggc
     4741 tagcgccgat gcatcctaat aagaggttct cactgatgat caagtagaaa acatccatac
     4801 tgcgattacc taaagccagc atcgtgccaa actccccggt tctttcatag atactcatat
     4861 tgacactatt ggcgacactt aaacatacca ttacaagaat gatcaattgc agtacaccaa
     4921 attgactttt atacaaatca actgttttct cataaaaatc attgagttca gtccaagttc
     4981 taacttcaag atcaaaggaa tctagcttgt tttttagtaa agcagttatt tgatccgtgt
     5041 ctttagtttc ttttagtgaa atcacgagcg catttacggc attaatactc aggagttcct
     5101 gcgctgttgc tagcgaaatt ttgatggtgc gcgcgtcata atcatttgaa aaggtttgga
     5161 atataccgat tacttcaaaa tctaagctgt tcaaagtgcc ttcaattgta tttgtaagta
     5221 aggtaaccgt atcacctggt ttaagtttta gtccattggc taacccacgc ccgagcataa
     5281 ggccaaattt atcctggtca gttaactgtc ttccgtcagt gatttttagt gcagtactaa
     5341 actgaccttc attttcaggg tgaatgcctt caccaacgat tggtaggtca ctacgaccat
     5401 tatttaataa gccagaaaaa tttagccgag ctgtaatact gtcaaatttt tcgacagtta
     5461 tttgctgagc aacagttttt ttcatttgat caaaatcata aataagatat ttctcagggg
     5521 agcgagaacc ttcataaaaa tacccttgct tatatatttg aatatgccca gattgtgagt
     5581 gaatcagagc ctcacctagt tggataaaaa tatcattaac ccacccacca gccaaaatga
     5641 ttccaacaac accacaaata atcgagatta aactcattgt ggtatgaatc ttttgtctca
     5701 gaatgttgcg taagctaagt ttaagcattg atttttatcg gttatggcga agcgcatcga
     5761 cgatttgcaa ttttgaagct ttccaagccg gataaacact tgcaataaga gttgttccta
     5821 tcgctaatga tatagcttct aataccattg attgagtaac taatatttcc cctatgtatc
     5881 ctcttgccat gccgggggga gggggcattg gtatcccgac acttgaaata atattcgcta
     5941 ataaataacc gatcattaca ccaagtatgc caccgatgca tccaagcagg ataccttccg
     6001 ttaggaacat gtacagaatt tttgtttgtt tgattccaag tgccattgct gtaccgattt
     6061 ctccggttcg ttctatcaca ctaatactca tcgtgttgga aatacttaac aaaatgatta
     6121 ttgcgataat gattttgatt gctagcactt gtttagaaag gagcacggtt gttttattat
     6181 agaagtcagc aagctgatac caaggtgtaa tctcaaactt actttgggga agctgatcgt
     6241 gcagtaaaga aaggacttga tttgtatctt cagtatgatt aagcaaaacg atccaactat
     6301 gtgcgcctgt cacacgaatg agtttttggg ctgtctcgat aggaattcga attgcattat
     6361 cgtcgtatga ttttgtgaca gttgtaaaaa gcccgctaat ggttacttca atcgcattaa
     6421 ttcctccaga ggctgtattt accagtaatg taacgttatc tccagtttct acgcccaggt
     6481 tgcgcgataa accttctccc ataataatct ggaatgtatt atttccagac aaataatttc
     6541 cggtagtgag ttgaagtgcg tcaccaaaga agttctgctc gtttggatca accccttcgc
     6601 caataaacga tagcgtcgat tcgccactgc tgattaaccc gctaaaagat aagcgtggga
     6661 caataacttt aattttctct aagctacttg atgtgagctt taattctggt tgtgtatcag
     6721 gtaatagaaa atgataagga tttgctttac cttcttcttg ataaccggga cgtgtgattt
     6781 gtaaatgacc taactgagat cgaatcgaag tttcccggaa gtcgataaaa atccactcaa
     6841 taaaaccact tgctaaaatg agtgaacaaa cgccaaatat gatggcccct aatgaaatta
     6901 aacttcgttg tttttggcga atgatattgc gaaatgctag gcttatttcg atggatttct
     6961 cccaattact gttcattcta gtataagagt tattaatatg gttatgaaaa aagataacga
     7021 tataataacc aagatgtact atatctttgt gttgattaat gggtgaaaaa acaaataaac
     7081 tagatggtaa agttaatttt ggcgtaaaac gggatttttt tctttatcag gatgccagct
     7141 ggtgagattg gaaaatgcga ttttagaatg gaagaaaatt atcggtgact ctcaggtttt
     7201 tgataaggag actgctgagg ttgaatatgg ttcatgtaca acaggagtaa gccgtgccat
     7261 ctctgctagt attaagccac ttgatgctag ccatattcca aatatcgtta agattgcgaa
     7321 taagtataag gtggcacttt atcccatcag tactggacat aactggggtt acggaacagc
     7381 gctcccatca attgacaatt gtgtaatttt agatttatca gcactaaaca aaattctgga
     7441 gtttgaccca gacactggtt tggtaacgtt agaacccgga gtcactcaag ggcaactagc
     7501 ggaatttctt acccttggaa atcactctta tatggttcct gtaacaggtg ctgggccaac
     7561 gtgcagctta cttgggaacg ccttagaacg aggctatgga atcactccga taacagatca
     7621 ttttggtgca gtaatttcta ttgaagcagt acttgcggat ggtagtattt atcaatctgc
     7681 cctctctgct atgggaggag aatcaattga ccgcgccttt aaatggaatg ttggacctta
     7741 cttagatgga attttctcac aaggaagttt tggtgttgtg acaaaaatga ccattgcact
     7801 tgcacatcgc ccagaatccg tggagtcatt cttgtttggc cttaaaaatt ctgagcaatt
     7861 accggacttg gtaagactta ttcaaaaaat tattacacgt tatccaggta ttttaggtgg
     7921 tgtgaattta atgaatgctc atcgcgtctt ggcgatgagc attccgtacc ctcgagataa
     7981 attagcacta aataagacta ttgcacaaga aacactttct gaactttgta aggctaatca
     8041 tgtaatgcct tggacgggtc tagggacact ttatgggtcg agaaaagtat tgaaggtggt
     8101 taaacaagaa ataaaagcaa tgttaagccc aatagcatca caattgattt ttgtaaattc
     8161 tggtttgata aaaacttcga ttaaagtcat aaatttcttt ccaaacttta ttcggaccaa
     8221 gttcgaaaga aaattaatga tgttagagcg ttcaatgcaa ttggtttcgg gttggcctaa
     8281 tcagactgca ttgcccctgt gctattggtt aatgaatgat tcagatgtta ggcaagaagt
     8341 ttttctgaat cctgccaggg atggatgtgg attaatttgg tattcaccat tagtccttat
     8401 gaagcctgac agtgtcatgc agtatataaa catggtgact tccgtcatgc aaaagcatgg
     8461 attagagcca ctcgttactt tgacaagtgt gacaaacgct tgctttgata gcacagttcc
     8521 cttgttattt gatcgtacat cgagtcaagg tcaaaagagt gcagaaaact gttattggga
     8581 gttattagaa acggggaaga agcttgggtt tgttccttac cgatttggta ttcatgcgat
     8641 gagctggttg actgctgatg aaacgcccta ttggcaaacc gttcgtaaaa ttaaacgatc
     8701 acttgatcct gacaatatta tttcgccagg taggtatgtt taatcttatt ttctctaggt
     8761 aattctgtcg atttttctta cacttttatt tttctaaaat ttggattggt tgctgctgct
     8821 gttgtaatgc attgttctgg ttgtgattta ttaatttatt aagattttgg catacaagtt
     8881 gcttttcttt ttgtgatggc agataatttg tcggtcataa ctcagtgata ggaaaactaa
     8941 ggagattcga atgattaaac aattaaaaaa aacagcgatt gcggcaatag tgggaggtgg
     9001 ttttc
//
LOCUS       DAOQLJ010000089         8988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3031_length_8988_cov_7.049703, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000089 DAOQLJ010000000
VERSION     DAOQLJ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8988
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3031_length_8988_cov_7.049703"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PK522_07320"
     CDS             complement(<1..495)
                     /locus_tag="PK522_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNC41580.1"
                     /translation="MEKEQAIKFMHDLLRLMLNKKASDLFITAGYPPAMKIDGKMTPV
                     SQQQLTAQHTVELVRAIMNDKQVGEFEASKECNFAIHPQGIGRFRVNAFIQQQRAGVV
                     IRTITTQIPKFDDLKLPEVLKDVVMSKRGLVIFVGGTGSGKSTSLAALIGHRNQDSYG
                     HIITI"
     gene            complement(529..1572)
                     /locus_tag="PK522_07325"
     CDS             complement(529..1572)
                     /locus_tag="PK522_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNC41581.1"
                     /translation="MNIVELLAFVVKNNASDLHLSAGMPPMIRVHGEIRRINLPAMEH
                     KDVHDMIYDIMNDSQRKHYEEHLECDFSFAIPNLARFRVNAFNTQRGAASVMRTIPSK
                     VLSLEQIKAPKIFAEIAQQPRGVVLVTGPTGSGKSTTLAAMINDINESNYGHILTLED
                     PIEFVHESKKCLINQREIGRDTHSFANALRSALREDPDIILVGEMRDLETIRLAMTAA
                     ETGHLVFGTLHTSSAAKTIDRIIDVFPAEEKEMVRAMLSESLRAVISQTLLKTKDGNG
                     RVAAHEIMIGTPAIRNLIREGKVAQMYSTIQTSQAVGMQTLDQNLADLVKRNVVSNAE
                     ARTKAMNKDNFRG"
     gene            1766..2482
                     /locus_tag="PK522_07330"
     CDS             1766..2482
                     /locus_tag="PK522_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNC41582.1"
                     /translation="MDTIATRLESVKLRIAEAAKLAGRDPQEILLLAASKTNQPEKLR
                     EAWNAGQKVFGENYLQEGLIKIHALSDLPIEWHFIGPIQSNKTKPIAENFAWVHSIDR
                     EKIAARLSSARPESLPPLQVCVQVNVSGEVTKSGIAPEDAIALAKFVSQQPRLKLRGL
                     MAVPELTSATALQREQFQIMRNIFDQLKNQGFDVDTLSMGMSEDMNIAIAEGATIVRI
                     GTAIFGPRRYAIPEELAPRQ"
     gene            complement(2451..2720)
                     /locus_tag="PK522_07335"
     CDS             complement(2451..2720)
                     /locus_tag="PK522_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HNC41583.1"
                     /translation="MALIITDECINCDVCEPECPNGAISQGEEIYEIDASRCTECVGH
                     YDKPQCIEVCPVNCIVTNPDLPESKDQLLAKYHRLITGEGQAPQE"
     gene            complement(2736..3215)
                     /gene="coaD"
                     /locus_tag="PK522_07340"
     CDS             complement(2736..3215)
                     /gene="coaD"
                     /locus_tag="PK522_07340"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNC41584.1"
                     /translation="MNKVIYPGTFDPITRGHEDLVRRASTLFEEVVVAVAGDTTKTPF
                     FSLEERISMAKTVLSDYANVKVIGFSGLLMSFVQRQNSRIIVRGLRAVSDFEYEFQLA
                     GMNRSLYPDVETVFLTPSEQYMFVSATIVREIARLGGEADKFVHPIVAEQLRRKLEH"
     gene            complement(3184..3759)
                     /gene="rsmD"
                     /locus_tag="PK522_07345"
     CDS             complement(3184..3759)
                     /gene="rsmD"
                     /locus_tag="PK522_07345"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNC41585.1"
                     /translation="MPQSGKVRIIGGQWRSRLISVANEVGVRPTPNRVRETVFNWLGQ
                     DLQGKHCIDLFAGSGAMGFESASRGAESVVMVEIKDNVFQVLRSMKEKLGAAQIKLVK
                     MDAIEFIRSDQNQYDVIYLDPPFQLGLLPKILPLLPSRLSKSGIVYLESEYPFQQNDH
                     WVILKCGRAGNVFFQLLKFEDHEQSNLPGHI"
     gene            4016..5263
                     /locus_tag="PK522_07350"
     CDS             4016..5263
                     /locus_tag="PK522_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HNC41586.1"
                     /translation="MSPYELLIGLRYTKAKRRNHFISFISLISLLGITLGMTALITVM
                     SVMNGFHKEVRSRILGVASHVQITSFNGTIAHWEQVADIAVKNPQVVSAAPYVNAQGM
                     LSYNQVVRGVIVRGILPDAENTVADFSNMMMMGELNQLQAGEFGIVIGLELARALGTF
                     PGDKIVLISPQGQVTPAGILPRLKQFTVIGVFEAGHYEYDSGLALIHLVDAQKLYRME
                     PDQVSGVRLKLHDLFEAPKVVRELSALFSSDTYISDWTQQHTNYFRAIQIEKRMLSLI
                     LALIIAVAAFNIVSTLVMAVTDKQSDIAILRTLGASPGSIMKIFIVQGTLIGLFGTLL
                     GIIGGVILAYNVSDVVVFIEQMFNIQFLSQEVYYISEIPSDPHLSDIVTVAVVSFVLT
                     LLATLYPSYRASKVNPAEALRYE"
     gene            5256..5936
                     /gene="lolD"
                     /locus_tag="PK522_07355"
     CDS             5256..5936
                     /gene="lolD"
                     /locus_tag="PK522_07355"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HNC41587.1"
                     /translation="MSNQVIACQNLYKSYFQGKLEVPVLHGVNLQLNKGEMVSIVGAS
                     GSGKSTLLHLLGGLDSPTKGQIKILDQDITQIDEQQRCQLRNQSLGFIYQFHHLLPEF
                     SAQENVAMPLLIRRMNRKEAMDTAAEMLQQVGLGHRLTHTPGELSGGERQRAAVARAL
                     VTRPACVLADEPTGNLDRHTAEGVFNLMLDLNQKANASLIIVTHDSQLAQRAERILHL
                     VDGVLTTQ"
     gene            complement(5977..6714)
                     /locus_tag="PK522_07360"
     CDS             complement(5977..6714)
                     /locus_tag="PK522_07360"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNC41588.1"
                     /translation="MLLFPDNVVNVNNPSRPIQLVLLRHGQSIWNQDRHFTGWSDVAL
                     SEKGKEEAKRAAHLLKQANFSFDLCYSSVLERAVDTLNIIRAEMGLERTPLIQDWRLN
                     ERHYGALEGLQRWEAIKKFGIWPVLACQIFYKAMPPILELNDLRFPGNQLRYQSINQQ
                     LLPQTESMEQTLARVYPCWQNDILPEIKRGKRILIVSHRNLLRTLVMQLNHLSPWHVM
                     KLSIATGKPLCFELDDQLNSIRSYYLQ"
     gene            complement(6695..7738)
                     /locus_tag="PK522_07365"
     CDS             complement(6695..7738)
                     /locus_tag="PK522_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNC41589.1"
                     /translation="MSDRSSVDFPLREAYALVSDLMTPDPRIYWVDFLFHILLGWTGF
                     IVTLQTTLFSIEQILGFVITVFTFYRSAIFVHELAHLKKGTFQVFRWVWNLICGIPMM
                     IPSFMYDGVHNDHHKRDVYGTHLDGEYLPFATQKPIEMVRYVLLSFILPLLLVARFLL
                     LTPLSYLIPPLRKLVWQRASSLTINPQYIRAPNAIRNDHYWWLQEFATFAFAATVVGL
                     VIVGLLPYQALVLWYVTAVCVFILNSLRTLAAHAYRNPGDHSLELAEQYLDSINIPGN
                     FLTTLWAPVGLRFHATHHLFMSLPYHNLGKAQQRLVNGLSDNSLYLKTIRKGLWDALK
                     RIWKEASIASISR"
     gene            complement(7740..8165)
                     /locus_tag="PK522_07370"
     CDS             complement(7740..8165)
                     /locus_tag="PK522_07370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNC41590.1"
                     /translation="MQKFGAPDTIIRQYQHWSVLLRPAQLTLGALVLVAHEPVFAFSE
                     LSSASFSELHQVTREIDSALKNAFGYDKLNYLMLMMVDPDVHFHVIPRYEAARSFNGQ
                     DFFDAGWPGVPNFSQVNQTDRVINQLIIDHITKHWKSTL"
     gene            complement(8194..>8988)
                     /locus_tag="PK522_07375"
     CDS             complement(8194..>8988)
                     /locus_tag="PK522_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HNC41591.1"
                     /translation="TKAIILSAGQGRRLLPLTENTPKCLLSVADKPVIAWQIDALIDA
                     GIDSITIVGGFQIGKVEALIAQRYAAYPEIRVCFNPFYEVADNLASCWIVRDQMEGNF
                     VLINGDTLFETALVAHVLQSEYAPITLSVDYKETFDEDDMKVELNSDKWVRHVSKLLV
                     PEQSHAESIGFIYFQQEGAILFRNAVEQALRDPSKLKSWYLSIIDQLAEQHQVNSRSV
                     QGFKWGEIDFIEDLQRAEKLFSTLDNKLNRYQQESSLRETIIPLPM"
BASE COUNT         2668 a         1921 c         1814 g         2585 t
ORIGIN      
        1 aatggtgatg atatggccat aactgtcctg attccggtga ccaataagcg cagccaacga
       61 agtcgactta ccagagccag tgccgccaac aaagatgact aatcctcgct tgctcattac
      121 gacatctttc agtacttcag gtaacttcaa atcatcaaat ttcggaatct gcgtggtaat
      181 cgttcgaatg acaacgcctg ctctttgttg ttgaataaat gcattgacgc ggaagcgccc
      241 aataccttgt ggatggattg cgaagttaca ttctttgctt gcttcaaatt ctcccacttg
      301 cttatcgttc atgatagctc gtacaagctc tactgtatgt tgcgctgtaa gttgctgctg
      361 tgatacaggg gtcatttttc catcaatttt cattgcggga ggatatccag cggtaatgaa
      421 cagatcagaa gctttcttat ttaacattaa gcgcagtaaa tcatgcataa actttattgc
      481 ttgttctttt tccataattt tatctactga taatgatcaa ttaaggtgtt atccacggaa
      541 attatctttg ttcatagcct ttgttcttgc ttcagcattt gagacaacgt ttctttttac
      601 gagatcagcc agattctggt cgagggtctg cataccaact gcctgactcg tctggatcgt
      661 tgaatacatt tgagctactt tcccttctcg aattaggtta cgaatcgcag gagtaccaat
      721 catgatctcg tgtgctgcaa ctcgaccatt accatcttta gttttcaata aagtttgaga
      781 aattacagca cggagcgatt ctgataacat tgcccgaacc atttcttttt cttctgctgg
      841 aaaaacgtca ataatacgat caatcgtttt agcggctgag cttgtgtgta gggttccaaa
      901 gactaggtgc ccagtttctg cggcagtcat agctaagcga attgtttcca aatctcgcat
      961 ttcaccgact agaataatat ctggatcctc acgcaaagct gaccgaagcg cattagcaaa
     1021 actgtgagta tctctaccaa tctctctttg attaattaag cattttttac tttcgtgtac
     1081 aaactcgatt ggatcttcaa gtgtaagaat atggccataa ttactttcat tgatatcatt
     1141 gatcatcgca gcaagtgtgg tcgatttacc agaaccggtt ggtccagtta ctagtacgac
     1201 accgcggggt tgttgcgcga tttcagcaaa aatcttgggg gccttgattt gctctagtga
     1261 tagtactttg gatggaatcg ttctcattac cgaagctgca ccccgttgtg tgttaaaggc
     1321 atttacccgg aagcgagcga gattgggaat agcaaaagaa aagtcgcatt ccaaatgctc
     1381 ttcataatgc tttctttgac tatcattcat gatgtcgtag atcatgtcat gcacatcttt
     1441 atgctccatt gcaggaaggt taatgcgtct gatttcacca tgaacacgaa tcatgggagg
     1501 catgccagca gaaaggtgca aatctgaagc gttattcttg accacgaagg cgagtagctc
     1561 tactatgttc atctattatt cctagtccaa gttgttaggt tatactgtga ttacttcagt
     1621 atgttttacc caattccaag taaaatcctg cttgtttatc aacggtaggc ctaaacaaaa
     1681 attgagaagc tactgctagc gtagttaatt tagaattttc cactccaaat acttacattt
     1741 tcgttttatt ttttacttca tcactatgga tacaatcgca acacgtttgg aatcagttaa
     1801 attgcgcata gctgaagctg caaagcttgc aggaagagat ccacaagaaa ttctattatt
     1861 agcggcaagc aaaacaaatc agccagagaa actgcgtgaa gcatggaatg ctggtcagaa
     1921 agtatttgga gagaattatt tacaagaggg actaataaaa attcatgcgc tttctgatct
     1981 tcccattgag tggcatttta ttggcccaat ccagagcaat aaaacaaaac caattgctga
     2041 gaatttcgct tgggttcaca gtatcgatag agaaaaaatt gctgctcggt tgtctagcgc
     2101 tagaccagaa tcattaccac cgctacaggt ttgtgtgcaa gtcaatgtaa gtggagaagt
     2161 tacaaaaagc ggcatagccc cagaagacgc gattgcattg gctaaatttg ttagtcaaca
     2221 accacgttta aaattgcggg gtttaatggc agtacctgaa ttgacttcgg ccacggcttt
     2281 gcaacgcgag caatttcaaa tcatgcgaaa catattcgat caacttaaaa atcagggctt
     2341 tgatgttgat acactatcca tgggcatgtc agaagatatg aacattgcca tagcagaagg
     2401 tgcaactatt gtccgtatcg gaacagcaat ttttggaccg agacgttacg ctattcctga
     2461 ggagcttgcc cctcgccagt aattaaccga tgatatttgg caagcaattg gtctttactt
     2521 tcaggcagat caggattcgt aacgatacag ttcaccggac aaacctcgat acattgcggt
     2581 ttgtcataat gcccaacaca ttctgtacac cgacttgcgt cgatttcata aatttcttcg
     2641 ccttgcgaaa tagctccatt agggcattct ggttcacaaa catcacagtt aatacattcg
     2701 tcagtaataa taagcgccat attattttta tttaatcaat gttctagttt tctacgtagc
     2761 tgttctgcca caatgggatg aacgaattta tcagcttctc cacctaatct cgcaatttcc
     2821 cgaacaatcg ttgcggaaac aaacatatat tgttcagaag gtgttagaaa tacagtttcg
     2881 acatcaggat aaaggctacg gttcattcct gccaattgga attcatactc aaaatcagag
     2941 accgcacgca gtcctcgaac aataattcga gaattctggc gttgtacaaa tgacatcaat
     3001 agcccagaaa aaccgattac tttcacgtta gcgtaatcgg atagcacggt ttttgccata
     3061 ctaattcgtt cttcaagtga gaaaaaaggt gtcttggtgg tatcacccgc aacagcaact
     3121 accacttcct caaacaaagt cgaagcacga cggactaaat cttcatgacc gcgtgtaatg
     3181 ggatcaaatg tgcccgggta aattactttg ttcatgatct tcaaatttta agagctgaaa
     3241 aaaaacatta ccagcacgcc cacacttcaa aataacccag tgatcattct gttgaaatgg
     3301 atactcactc tctagataaa cgattccaga tttacttaat cgcgatggta atagtggtaa
     3361 tattttagga agtaaaccca gttgaaaggg tgggtcgaga taaatcacat catattggtt
     3421 ttgatccgat cggatgaatt caatcgcatc cattttaacc agtttaatct gtgctgcgcc
     3481 taatttttct ttcattgagc gtagcacttg aaaaacatta tcctttatct ctaccataac
     3541 aacactttct gctccacgag atgccgattc gaaccccatg gcaccactcc cagcaaataa
     3601 atcgatacaa tgcttaccct gcagatcttg ccctagccag ttaaaaacgg tttcacgaac
     3661 tcgattcggt gtgggtcgaa caccaacttc atttgcaaca gagatcaaac ggcttctcca
     3721 ttgccctcca ataatacgaa cttttccact ttgaggcatt agctgataat atctattctg
     3781 aatggacaga aaagcgctga tttaagtaat ttgagttact taatcatcca attgctagca
     3841 ttatactgaa caaaaaataa ttgaataacc actagctaat caatttaagg caatgagaaa
     3901 gcgtattcat cgtttagaaa cagagctgct ttaattttaa accatataac atcactattg
     3961 ataatagctt ttcttagaaa acaacatcat ctaaatcttt ttcaggaatt catttgtgtc
     4021 accttatgag ctattaatag gcctacgcta cacaaaagca aagcggcgta atcattttat
     4081 ttcctttatt tccttgattt cactactggg tattacactg ggtatgacag cgctaattac
     4141 ggtcatgtca gtcatgaatg gatttcataa agaagtgcgt tcgcgaatat tgggggttgc
     4201 ttctcatgtg caaatcacaa gcttcaatgg aacgatcgcg cattgggagc aggtcgctga
     4261 tatcgcagtc aagaatcctc aagtagtttc ggcagcaccc tatgttaatg cacaggggat
     4321 gctttcgtat aatcaagtgg tacgcggtgt tatcgtgcgc ggaatattac cggatgctga
     4381 aaatactgtc gcagatttct ccaatatgat gatgatgggg gagttaaatc agctacaggc
     4441 tggagaattt gggattgtca ttggccttga attagcgaga gcgctgggta cttttccagg
     4501 tgataagatt gtgttgatat cacctcaagg tcaagtaacg ccagcaggta ttttaccccg
     4561 cttgaaacaa tttaccgtta tcggtgtttt tgaagccggt cattatgaat atgattcagg
     4621 tctcgcgtta atccatctgg ttgatgcaca gaagctctat cgcatggaac ctgatcaagt
     4681 atccggtgtt cgcttaaaat tacatgactt gtttgaggca ccgaaagtag tgcgagaatt
     4741 gtcagcccta ttttcatcgg atacctacat tagcgactgg acgcaacaac ataccaatta
     4801 ctttcgtgca attcaaatcg aaaagcgcat gctgtcactc attctggcac taattatcgc
     4861 agttgcggcg tttaacattg tttcgacact cgtcatggct gtcacagata aacaatcgga
     4921 catcgccatt ttacgcacgc tcggcgctag cccgggtagc atcatgaaaa ttttcattgt
     4981 acaaggaaca ttgatcggct tgttcggaac cctattaggc attataggtg gagttattct
     5041 ggcttataac gtcagtgatg tcgtagtttt cattgagcaa atgtttaaca ttcaattcct
     5101 ctcccaagaa gtatattaca ttagtgagat tccttcagat cctcatttat ctgacattgt
     5161 aactgtagcc gttgtgtcat ttgttttgac attgttagcg acactttatc caagttatcg
     5221 cgcatcaaaa gttaatcctg ccgaggcatt gcgttatgag taaccaagtt atcgcttgcc
     5281 aaaatttata taagagttat tttcagggaa agctagaagt acccgtatta cacggggtaa
     5341 atctgcaact aaacaaaggt gaaatggttt cgatcgtcgg tgcttctggc tcgggcaaaa
     5401 gcaccttact acacttactt ggagggttag acagtccaac caaaggtcag attaagatat
     5461 tggatcagga tatcacccag atcgatgagc aacagcgatg ccaactacgc aatcaatcat
     5521 tgggctttat ttatcaattt catcatttac ttcctgagtt tagcgcacag gaaaatgttg
     5581 cgatgccact tttgattcgg cgcatgaata ggaaagaggc tatggatact gctgcagaaa
     5641 tgcttcagca agttggactt ggtcatcgac tgactcatac cccaggagaa ctttctggcg
     5701 gtgaacgcca gcgtgcggcg gtagcaaggg cattggtaac aaggccagcc tgtgttttag
     5761 cggatgaacc tacgggtaat cttgatcgac ataccgctga aggggtattt aatttaatgc
     5821 tcgacctcaa tcaaaaagca aatgccagtt taatcatcgt aacacatgat tcacagctcg
     5881 ctcagcgagc agaacgcatt ttgcatctgg ttgacggcgt tcttacaact cagtaatttt
     5941 ttacccaata caaccttatt ctgaattgac cccttattac tgaaggtaat agcttctgat
     6001 agagtttaac tgatcatcta actcaaaaca gagtggcttc ccggtcgcaa tagagagctt
     6061 cataacgtgc cacggtgata agtgatttaa ttgcattacc agcgtgcgta aaagatttcg
     6121 atgtgaaaca atgagtattc gtttacctcg cttaatttcc ggcagaatat cgttctgcca
     6181 acacggataa acacgcgcca gggtttgctc catactttca gtttgtggta ataactgttg
     6241 gttaatactt tgatagcgta actgattccc tgggaagcga agatcgttca attcaagtat
     6301 cggcggcatt gctttgtaga agatttgaca agccaagaca ggccaaatac cgaatttttt
     6361 aatcgcttcc catcgttgaa gaccttcaag tgcgccatag tgccgttcat ttaagcgcca
     6421 atcttgaata agtggtgttc tttctaaccc catttcagcg cgtataatgt tgagggtatc
     6481 aaccgctcgt tccaatacag agctatagca aaggtcaaaa ctaaaattgg cttgtttaag
     6541 caagtgtgcg gcacgcttgg cttcctcctt gcctttctcg cttaatgcaa catcactcca
     6601 acctgtgaaa tgcctatctt gattccagat actctgccca tggcggagca aaactagttg
     6661 tatcggcctg gaagggttgt tcacattaac aacattatcg ggaaatagaa gcaatggatg
     6721 cttctttcca aattcgcttc aatgcgtccc ataatccctt acggattgtt ttcaaataaa
     6781 gcgagttatc cgataaccca ttaactaatc gctgctgcgc tttacctaaa ttatggtagg
     6841 gcaaactcat aaaaagatgg tgggttgcat ggaaccttaa tccaacagga gcccaaagcg
     6901 tagtcagaaa atttcctgga atattgattg aatctaggta ttgctctgcc aactcaagcg
     6961 aatgatctcc tggattacga taagcatgtg cagccagtgt tcttaacgaa ttaagaataa
     7021 aaacacatac cgcagtcacg taccataaaa ctaaagcttg gtaggggagt aaccctacaa
     7081 taaccagccc tacgacggtt gcagcaaatg caaatgttgc aaattcctgt aaccaccaat
     7141 aatgatcatt acgaattgca ttgggtgccc gaatatactg gggattaatc gttaatgatg
     7201 aggcacgctg ccaaacgagc ttacgcagcg gcggaatcaa ataagatagc ggggttaaca
     7261 ataagaaacg cgcaaccagc aatagcggaa gaataaagga gagtaataca tagcgaacca
     7321 tttcgatggg cttctgcgtg gcgaatggaa gatattcccc atccaaatga gttccataga
     7381 cgtcgcgctt atggtgatca ttatgcacac catcatacat gaaggaagga atcatcatcg
     7441 gaatgccaca gattaagttc catacccatc ggaaaacttg gaaagtcccc tttttaagat
     7501 gtgcaagttc atgaacaaaa attgcagaac ggtaaaaggt aaaaaccgtg atcacaaatc
     7561 ccaaaatttg ctcaattgaa aaaagcgttg tttgcaacgt aacgataaat cctgtccatc
     7621 ccaataggat atgaaacaaa aaatcaaccc aatagattct tgggtcaggc gtcattaaat
     7681 cactgacgag tgcatatgct tcacggagcg gaaaatcgac agaggaacga tcagacatct
     7741 tataaagttg atttccaatg cttggtaatg tggtcgatga ttaactgatt gatgacacga
     7801 tcggtctggt taacttggct aaaattggga acaccaggcc atcctgcatc aaagaaatct
     7861 tgcccattaa atgatcttgc agcttcatag cggggaataa catggaagtg aacatcagga
     7921 tccaccatca tcaacataag ataatttaat ttatcgtaac caaaagcatt tttcagtgca
     7981 ctatcaattt ctctggttac ttggtgtaat tcactgaagc tagctgaact taattcagag
     8041 aatgcaaaga ccggctcatg agcaacaagc accaacgcac ccaaagttaa ttgcgcaggg
     8101 cgcaataaaa cactccaatg ctgatattgg cgaatgatgg tatccggcgc accaaatttc
     8161 tgcatcgtac cattaaacac agtcaatgcc atattacatc ggaagcggaa taatcgtctc
     8221 gcgaagggag gattcttgct gataacgatt aagcttattg tcaagggtag agaatagctt
     8281 ttctgcgcgt tgtagatctt cgatgaaatc gatttcgccc catttaaatc cttgaactga
     8341 tctcgaatta acctgatgct gctcagcaag ctgatcgata atactcaaat accatgattt
     8401 aagtttactg ggatcgcgaa gcgcttgctc aaccgcattc cgaaacagta ttgctccttc
     8461 ttgctggaaa taaataaagc caatcgattc tgcatgagat tgttccggca ccagtaattt
     8521 actgacatgc cgaacccatt tgtcactatt gagctccact ttcatatcat cttcatcaaa
     8581 tgtttcttta taatcaacac tgagcgttat aggcgcgtat tcagactgaa gaacatgggc
     8641 aaccaaagca gtttcaaata gcgtatcacc attgataagg acaaaattac cttccatctg
     8701 gtcgcgcaca atccagcaac tggccagatt atctgctact tcataaaatg gattaaagca
     8761 aacacgaatc tctggataag cagcatatcg ttgagcaatc aatgcttcga cctttccgat
     8821 ttgaaaccca cctacaatcg ttattgaatc gataccagca tcaatcaatg catcaatttg
     8881 ccaagcaata acaggtttat ccgcaacaga taataagcac ttgggagtat tttctgtgag
     8941 cggtaacagt cttctgcctt gtcctgcact taatataatt gctttggt
//
LOCUS       DAOQLJ010000090         8903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3081_length_8903_cov_6.317699, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000090 DAOQLJ010000000
VERSION     DAOQLJ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8903
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3081_length_8903_cov_6.317699"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PK522_07380"
     CDS             <1..493
                     /locus_tag="PK522_07380"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNC41592.1"
                     /translation="YLDKDFIDSDHEIQLRTGVKIPLIFEIEGEAGFRKRETEVLQDL
                     VKLENIILATGGGVILSEENRRLLKQNGIVIYLCASVDELLRRTRTDKNRPLLQTEDQ
                     HARLTELLAHRDVLYRNTADIIIDSGKQGVRSLVYKLIRKLRTYDVKQQYAVPLEHAN
                     ERT"
     gene            529..1638
                     /gene="aroB"
                     /locus_tag="PK522_07385"
     CDS             529..1638
                     /gene="aroB"
                     /locus_tag="PK522_07385"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNC41593.1"
                     /translation="MANIETIKVHLVSSQSDRSYPIYIGSELLEQVEYILPYLRQKRV
                     AIVTNETVAPLYLKKIKDALEHHQVSTVAIILPDGENYKNWETLNLIFDALLKQNCER
                     KTTLIALGGGVIGDLTGFAAATYLRGIPFVQVPTTLLAQVDSSVGGKTGINHPLGKNM
                     IGSFYQPLLVLADSSTLFTLPDREFRSGLAEVVKYGLIRDAEFFNWLEVNMSKLVARD
                     LAVLNTAICRSCEIKAEIVAADEHESGVRSLLNLGHTFGHAIENAMGYGQWLHGEAVA
                     AGTIVAAELSKRMGLINETEFTRIRGLFELANLPISLPAISPSHYLDLMKLDKKVEGG
                     KMRFILLNQIGQAAIFDNIRESLLIETMLACGAND"
     gene            1631..2752
                     /locus_tag="PK522_07390"
     CDS             1631..2752
                     /locus_tag="PK522_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNC41594.1"
                     /translation="MIELAPYAISFQYSRGRLFKEDPPVGRSEFQRDRDRIIHSTAFR
                     RLEYKTQVFVNHEGDLFRTRLTHSLEVAQIGRSIARNLQLNEDLVEAIALAHDLGHTP
                     FGHAGQEALNRCMQEYGGFEHNLQSLRVVDFLEERYAAFNGLNLCFETREGILKHASL
                     KNARKLGEVGERFILKQRPSLEAQLTNLADEIAYNNHDVDDGLRSGLITLLQLQEIDI
                     FARHLHKVRKQYPDISGRRLIHETVRSMINSLAIDLTLQSKQNIAITKPASPEAVRTA
                     LPLIEFSEEISREQQVLKSFLRENLYKHFQVIRMSNKAQHTITRLFDAFSTEVRLLPN
                     EYQQKFSNYGHQAIADYIAGMTDRFAIKEYQRIFAISEN"
     gene            2788..3549
                     /locus_tag="PK522_07395"
     CDS             2788..3549
                     /locus_tag="PK522_07395"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNC41595.1"
                     /translation="MISIIQLTRMFLLAMLMFFLGLSAAWASNVESDELVDWASRGNF
                     TRIKTLFSETGKQPEQETLQRAYLVAAKNGHDKIVQFFLDQGVNIEVRDEKGYTALMY
                     AAEDDREQVMRVLLDAKAEINAANEEGMTSLLLASYRDRIEILRILIGAGANINAATN
                     KGITPTMLAAQNGYQIILQDLIKAKADLNSKAENGVTALMLAAQNGHQSATRTLIAGG
                     ANINIEAENGATALLLAKKYNHKGVIDLLVQAGAR"
     gene            3655..4344
                     /locus_tag="PK522_07400"
     CDS             3655..4344
                     /locus_tag="PK522_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC41596.1"
                     /translation="MNNFSKFLIYSLTSALFLILLVSCGDDSATSKGGAGNSSMKLMD
                     EVTSITELPSVLQESLSSEEKADLLKKILPMSDSSISPDEQFQKLREDASAGNAEAQN
                     GLGTLFYTGHAISRDASGKILDFDLESAAAWFYRAAEQGHAGAQFNLGLMYAEGQGVP
                     KDSAKAVELFTKAAEQGNVDAQNNLGVMYFVGEGVSRDVNKAKQWFEKAAAQGNEEAK
                     GNLEALQNAGS"
     gene            complement(4345..4950)
                     /locus_tag="PK522_07405"
     CDS             complement(4345..4950)
                     /locus_tag="PK522_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNC41597.1"
                     /translation="MDIITAIKSRRAIKSFDPNHILSDTEIEQLLSLAICSPTAFNIQ
                     NWRFVVIQDKALRKKIRAVSWDQAQVTDASLLIILTADLKSWEKQPERYWKNAPQSIR
                     DYIIPAIDHYYREHSQVERDEAMRSCGMAAMTLMLAAQGMGYASCPMDGFDFEAVSEL
                     IHLPSDHVIAMFVVIGKETKPAWPQGGQLPLEEIIIYNRFD"
     gene            5096..5689
                     /locus_tag="PK522_07410"
     CDS             5096..5689
                     /locus_tag="PK522_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HNC41598.1"
                     /translation="MSQFKKTLLCSLVSVFVSGLVVGQASAGQHDGHHGHHKTYAHFN
                     KDGELLTPKNYREWIFVGSPVTPKDMNDGNPAFPEFHNVYIDPTSWAHWKQTGTFRDG
                     TIIVKEMVSVGTKESSSGNGYFQGEFLGIAATVKDSKRFANSPNNWAYFGFASYESAS
                     AAAQPDDACAACHKANAAEDNVFTQHYPVLRAGKAKK"
     gene            5875..6453
                     /locus_tag="PK522_07415"
     CDS             5875..6453
                     /locus_tag="PK522_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNC41599.1"
                     /translation="MSNHQSSTGKIIAPELETIGWLNSAEPITLASLRGKVVLIHAFQ
                     MLCPGCVQHGIPQTKKIFEEFDPSKVAVLGLHTVFEHHAVMGRDALEVFAYEYRLSFP
                     IGIDKPNEHHVIPHTMMTYQMQGTPTTILIDKTGHLRMHKFGHVTDFVLGLSIGTLLA
                     EVDDESELASPSQQQNQKNLQSGACGPDECAV"
     gene            complement(6571..6978)
                     /locus_tag="PK522_07420"
     CDS             complement(6571..6978)
                     /locus_tag="PK522_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrosocyanin"
                     /protein_id="HNC41600.1"
                     /translation="MKLFKALCASIVLGLAVANVQAGEQNFTVVINAYDTTIPELNVE
                     GVTVKNIRAFNVLNEPETLTVHKGTSVKITVENKSPISEGFTIDGYGIQEVIKAGETK
                     TISFVADKAGAFTIWCQLHPKNIHLPGSLNVIE"
     gene            7187..8530
                     /gene="xseA"
                     /locus_tag="PK522_07425"
     CDS             7187..8530
                     /gene="xseA"
                     /locus_tag="PK522_07425"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNC41601.1"
                     /translation="MDHLSTASLNVHTLLSVSELNSYARALLESSFPLLWIRGEVSNL
                     KQYPSGHWYFLLKDKDAQIRCVMFRQKNQWLDWSLRDGMQIEVQALVTLYEARGEFQL
                     NVEHIRQAGLGNLYEAFEKLKSKLQAAGIFDAIHKKNIPEFPQRIGVITSLNTAALHD
                     ILSTLRHRMPSLPIIIYPTPVQGKDAAKSIVTMIELAGKRAECDVLLLCRGGGSIEDL
                     WAFNEEIVAYAIFHSVIPIVTGIGHETDFTIADFVADKRAPTPTGAAQIVSPDTQEIA
                     KHLNHLYHRIERAYKHEIERRMQTLDLLCHRLIDPRESIKHQVNRLQYLREKLLFCWS
                     RKIEKIDSENRHLQRRFFSSALNLEHMLNQQFERSSKLHKAINYYLERQGTKLVHYQT
                     QLTHLNPKSVLQRGYSITSNAEGKILRDSSQINIDDSIQVTLAEGWVKAKVSDAG"
     gene            8617..>8903
                     /locus_tag="PK522_07430"
     CDS             8617..>8903
                     /locus_tag="PK522_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNC41602.1"
                     /translation="MSRLFLALGAINAFLCVLLGAIGAHILKQKFSGELFNTFQTGLQ
                     YHFYHALGLILIGVLLFHLPQSKLVKLSGWVMLTGILFFSGTLYIVSFTEMR"
BASE COUNT         2725 a         1562 c         1934 g         2682 t
ORIGIN      
        1 ctatctggat aaagatttta tcgattcaga tcatgaaatt cagctaagaa ctggggtaaa
       61 gatcccatta attttcgaaa ttgaagggga agctggtttc cggaaacgtg aaacagaagt
      121 actccaggat cttgttaagc tcgagaatat tattttagcg acgggcgggg gagtaattct
      181 gagcgaagaa aatcgcaggt tattaaaaca aaatggaatt gtgatttatt tatgtgcatc
      241 cgtcgatgaa ttattgcgaa gaacacgtac cgataagaat agacctttat tgcaaactga
      301 agatcagcat gcgcgattga ccgagctgct agcgcatcgt gatgtgctct accgaaatac
      361 agcggatatc atcattgata gtggaaaaca aggcgtaaga tcgcttgttt ataaacttat
      421 tcgaaagctg cgaacgtatg atgtgaaaca acagtatgct gtgcctttgg agcacgcaaa
      481 cgaaagaacg taatagcagg caacggtaat acttagtacg aaattcccat ggcaaatatt
      541 gaaactatca aggttcatct cgtatcatct caatcggatc ggtcttatcc gatctatatt
      601 ggaagtgagt tattagagca agttgagtat attttgcctt acctgaggca gaaacgtgta
      661 gcaattgtaa ctaatgaaac cgtcgcacct ctttatttga aaaaaataaa ggatgcgttg
      721 gaacatcatc aagttagcac ggtagcaatc attttacccg atggagaaaa ttacaaaaac
      781 tgggaaacac ttaatttaat ttttgatgca ttgctgaaac aaaattgtga acgtaaaacg
      841 actttgatcg ctttgggtgg cggcgtgatc ggcgatctaa ccggttttgc tgctgctacc
      901 tatttacgtg gaataccttt tgtacaagtt ccaacgacct tattagcaca ggtagattca
      961 tccgtaggag gtaaaacagg aatcaatcat cccttgggta aaaatatgat tggttcgttt
     1021 tatcaacctt tattggtgtt agcggatagt tcaacacttt ttaccttacc agatcgagaa
     1081 ttccgatcgg gtcttgctga agtagttaaa tatggattga ttcgcgatgc tgaatttttt
     1141 aattggcttg aagtaaatat gagcaagcta gttgcacggg atttagccgt gctaaataca
     1201 gcaatatgtc gcagctgtga aattaaagct gagattgtgg cagcagatga gcatgaaagt
     1261 ggtgttcgtt ctttattaaa tttaggacat acctttggcc atgccattga gaatgcaatg
     1321 ggttatggac aatggttgca tggagaagcc gttgcggctg gaacgatcgt agccgctgag
     1381 ttatctaaac gaatgggatt gatcaacgaa accgaattca cacggattcg aggtttgttt
     1441 gaattggcaa atttgcctat ttctttgcct gcgatttctc caagtcatta ccttgatttg
     1501 atgaaattag acaaaaaagt agaaggtgga aaaatgcgtt tcattttgtt aaatcaaatt
     1561 ggtcaagcgg cgatctttga taatattcga gaatcccttt tgatcgagac gatgttagct
     1621 tgtggggcaa atgattgagt tagcccctta cgccatatcg tttcaatatt cgcgaggacg
     1681 tttattcaaa gaagatccac cagtagggcg tagtgagttt cagcgtgatc gtgatcgaat
     1741 tattcattcc actgcatttc gtcggttgga atataagaca caagtgtttg tcaatcatga
     1801 aggtgattta tttcgaacga gacttactca cagcttagaa gttgctcaaa ttgggcgctc
     1861 cattgcacgc aatttacagt taaatgaaga cttagttgaa gcgattgcat tagcccatga
     1921 tttagggcat accccatttg gtcatgcagg gcaggaagca cttaatcgct gcatgcaaga
     1981 atatggcgga ttcgagcata atctccaatc gcttagagtc gtagattttt tagaggagcg
     2041 ctacgctgct tttaatggat tgaatctctg ttttgagact cgagaaggaa ttttaaagca
     2101 cgcatctctc aagaatgcac gtaagctcgg tgaggttgga gaacgtttca tactaaaaca
     2161 aaggccttcg cttgaggcgc aacttacaaa tttggcggat gaaattgcgt ataacaatca
     2221 cgatgtcgat gatggtttgc gatcgggttt aattacgtta ctgcaattgc aggaaattga
     2281 tatctttgct cggcatttgc ataaagttcg aaagcaatat ccagatatat caggtaggag
     2341 gttaattcat gaaacggtgc gtagtatgat caatagtcta gcgattgatt taacgttgca
     2401 aagcaaacaa aacattgcaa taactaaacc agcaagccct gaagcggttc ggactgcgtt
     2461 accattgatt gaatttagtg aagaaataag ccgggagcaa caagtattga aatctttctt
     2521 aagagaaaat ctttataagc attttcaagt gatacggatg agcaataaag cacagcatac
     2581 gatcactcga ctttttgatg cttttagtac ggaggttcga ctgctaccta acgaatatca
     2641 gcaaaaattt tctaactatg gtcatcaagc cattgctgat tatattgccg gtatgaccga
     2701 tcgttttgct attaaggaat accaacgaat ttttgcgatt agtgaaaatt aagattaata
     2761 aaaagagaat cagtaaggag aaaaccaatg atcagcatta ttcagctaac ccggatgttt
     2821 ttattagcaa tgctaatgtt ttttttagga ttgagtgctg catgggcatc taatgtagaa
     2881 agcgatgagt tagtggattg ggcatcacgt ggtaacttta cccgtatcaa aacgttattt
     2941 tcagaaactg gaaaacaacc tgaacaagaa accctgcaac gcgcctatct ggttgctgca
     3001 aaaaatgggc acgataaaat cgttcagttt tttttagacc agggtgtcaa tatcgaggtc
     3061 agggatgaga agggctatac tgcattaatg tatgctgcag aagatgatcg agagcaagta
     3121 atgcgggtgc tattagatgc aaaagcagaa ataaatgcag ccaatgaaga gggtatgaca
     3181 tctttgctac tcgcatcgta tagagatcga atagaaattc tacgaatttt aatcggtgcc
     3241 ggggcaaata ttaatgcagc aacaaataaa ggaataacac ccaccatgtt ggcagctcag
     3301 aatggttatc aaatcattct ccaagattta attaaagcga aggctgatct taatagcaaa
     3361 gccgaaaatg gtgttacagc cttgatgcta gcagctcaaa atggtcatca atcggccaca
     3421 agaaccttaa ttgcaggggg agccaatatt aatatagagg cagaaaatgg cgcaacagca
     3481 ttattgctag cgaagaaata taatcataag ggtgtgattg atttacttgt gcaagcgggc
     3541 gctcgctagt tcattacaga gtgagattga acgagtagta atttgtagga tttatatctc
     3601 tgtaatagaa tctgagctta ttttaactgt agaacgaata ggtataggtt gattgtgaac
     3661 aatttttcta aatttctaat ttatagctta acgagtgctt tatttttaat tttattagtt
     3721 agttgtggtg atgattcggc aacgagtaaa ggtggtgctg gtaattcttc aatgaaattg
     3781 atggatgaag taaccagtat cactgaatta ccatctgtac ttcaagaaag cttgtcatct
     3841 gaggagaaag cagatctgct caaaaaaatt ctgcccatga gtgatagctc aatatccccg
     3901 gatgaacaat tccaaaaatt acgtgaagat gcaagcgcag gtaatgcaga agcacaaaat
     3961 ggtctgggca cactatttta cacagggcat gcgatttcaa gggatgcatc cggaaaaatt
     4021 cttgattttg atttagaatc agctgctgca tggttttatc gagcggcaga acaaggacat
     4081 gcaggagcgc aatttaacct tgggctcatg tatgcagaag gtcagggtgt cccaaaagac
     4141 tcagccaaag cagtagaatt atttactaaa gctgctgagc aaggaaatgt cgatgctcaa
     4201 aataacttgg gtgttatgta ttttgtcggc gaaggtgttt cgagagatgt caataaggcg
     4261 aagcaatggt ttgagaaggc agctgctcaa gggaatgaag aagccaaagg aaatctcgaa
     4321 gcgttacaaa atgcaggtag ttaatcaatc aaatcggtta tagataatga tttcttctaa
     4381 gggtaactga ccaccctgtg gccatgcagg ctttgtttct ttaccaatta ctacaaacat
     4441 tgcgatgaca tggtcactgg gtagatgaat taattcactt actgcttcaa aatcaaaccc
     4501 atccatagga caagaagcgt atcccatccc ttgagctgca agcattaagg tcattgctgc
     4561 cattccacaa gagcgcatgg cttcatcacg ttcaacttgg gaatgctctc ggtaatagtg
     4621 atcaatcgcc gggataatat aatctctgat ggattgaggt gcattcttcc agtaacgttc
     4681 aggttgtttt tcccaagatt tcaggtctgc tgtaagaatg attaataaag aagcatcagt
     4741 aacttgggct tgatcccatg aaacagcacg tatctttttt cttagcgctt tatcttggat
     4801 tacaacgaaa cgccagtttt ggatgttaaa ggctgttggt gagcagattg ctaatgagag
     4861 aagttgctca atctcggtat cagatagaat gtgattggga tcaaatgatt tgattgctct
     4921 gcgagatttt attgccgtga ttatgtccat ttataactat ccaatcatag aagaattatt
     4981 aaaataaatc ttaccttgac gagtttcgct accctgacta ccataaggcc aggagagcag
     5041 tcctatcgtt tgtctgtttt ctgtgaattc attacttaca ttttaaggat taattatgtc
     5101 acagtttaaa aaaacgttat tatgtagcct cgtttcagtc tttgtttcag ggttggtagt
     5161 tggtcaagct tctgcgggcc agcatgatgg gcatcatggt catcataaga cttatgctca
     5221 ttttaataaa gatggagaat tattaacgcc taaaaattat cgggaatgga tttttgttgg
     5281 ttctcctgta acacccaaag atatgaatga tggcaaccct gcatttcctg agttccataa
     5341 tgtctatatt gatccaacaa gttgggcaca ctggaaacaa accggaactt tccgagatgg
     5401 cacgattatc gttaaagaaa tggtgagtgt cggtaccaaa gaatcttcta gtggaaatgg
     5461 ttatttccaa ggtgaattct taggcattgc tgcaacggta aaagattcca aacgctttgc
     5521 aaatagccca aataattggg cttattttgg ttttgcgagc tatgagagcg caagtgcagc
     5581 agctcaaccc gatgatgcat gtgctgcatg ccataaagcc aatgcagctg aggataatgt
     5641 atttacccag cactatcctg tattgcgtgc aggcaaagct aagaagtgat ttaattattg
     5701 accatgctca tgggattcag agtggcgaaa gtctactcat aaagatctca tgttaatgcc
     5761 aagtattgct agagggcaca ttctttttta gtgattaata aaaagaagtt gccctttagt
     5821 tttttgaagt aaaaaaatga atagctgtgc ttttgatcaa gagtttatta caaaatgagt
     5881 aaccatcaat catccactgg aaaaattata gcgcctgagt tggaaacaat cggttggtta
     5941 aattctgccg aacccataac actcgcttca ctgagaggaa aggtggtgtt aatacatgct
     6001 ttccaaatgc tttgcccggg ttgtgttcaa catggtattc ctcaaaccaa gaaaattttt
     6061 gaggagtttg atcctagtaa ggttgctgtg cttgggttgc ataccgtgtt tgagcatcat
     6121 gccgtaatgg gacgtgatgc attagaagta tttgcttatg aataccgttt aagttttcca
     6181 ataggaatcg ataaaccgaa tgaacaccat gttattcctc ataccatgat gacataccaa
     6241 atgcagggaa ctccaacaac aattctaatt gataaaacgg gtcaccttcg catgcataaa
     6301 tttggccatg ttacggattt tgtactaggt ctttcgattg gtactttatt agctgaagtt
     6361 gatgatgaga gtgagttggc atctccgagt cagcaacaaa atcaaaaaaa tttacaatca
     6421 ggtgcttgtg gtccagatga atgcgcagtt tgatattgat aatcgatgaa aaataaaagc
     6481 cagaaattat ctggctttta ttatttaata tccgaaaaag taaggtgttt tgattgtatc
     6541 gctacaactt tattccttag tgtttttcaa ttattcaatg acattcaaag aaccaggtaa
     6601 gtggatattt ttaggatgta attggcacca aattgtgaaa gcacctgctt tatcagctac
     6661 aaaagaaatt gttttagttt cgcccgcttt aatgacttcc tgaattccat aaccgtcgat
     6721 agtgaaacct tcactaatcg gagatttgtt ttctacggta attttgacag atgtgccttt
     6781 gtgcacagtt aatgtttcag gttcattgag cacattgaag gcacggatat ttttaacggt
     6841 tacgccttca acattgagtt ctggaatcgt cgtatcatag gcattaataa caaccgtgaa
     6901 gttttgctct ccagcttgta catttgcaac ggctaatccc aatacgatac ttgcacataa
     6961 cgctttgaat agtttcatat accctccata gaatattttt tataaatagt tagtttaagt
     7021 ttgttgctta cgcttctcag tgaagcgtat ctaatatcta acataagata aatgagaata
     7081 gcaattaatc gcagttaaat attaggctga gaattatagc gcaagatttt attcatcgtt
     7141 atatttctct ataattttcc taagcttaaa ccaataagtt tgtcatatgg atcatctatc
     7201 gacggcgtca ctcaatgttc ataccttatt gagtgtttct gagttaaaca gctatgcgag
     7261 agcgctgctg gaatcatcat ttcctttgct atggattcgg ggtgaagttt caaatttaaa
     7321 acaatatcca tcgggccatt ggtatttttt actaaaagat aaagatgccc agattcgttg
     7381 tgtaatgttt cgacaaaaga atcaatggct agattggtca cttcgagatg gtatgcaaat
     7441 tgaagttcag gcacttgtaa ccttgtatga agcaagaggc gaatttcagc ttaatgttga
     7501 acatatccga caagctggtt tggggaattt atatgaagct ttcgaaaaat taaaatcaaa
     7561 attacaagct gcaggcatct ttgatgccat tcataaaaaa aatattccgg agtttcctca
     7621 gcggattggc gttattacat cgctaaatac tgccgcattg catgacattc tttcgacatt
     7681 acgtcatcgc atgccttctt taccgatcat tatttaccca acgccagtac aaggaaagga
     7741 cgcagcgaaa tcaatcgtga ctatgattga gttagcagga aaacgggcag aatgcgatgt
     7801 actattactt tgtcggggtg gcggctcaat cgaagatctt tgggccttta atgaagaaat
     7861 tgttgcctat gctatttttc atagcgtaat tccgattgtg acgggtattg gacatgaaac
     7921 tgattttacc attgctgatt ttgtcgcaga caaacgtgcg cctaccccaa ctggggcagc
     7981 acagattgtt tctcctgata ctcaagagat agcgaagcat ttaaatcatt tgtatcaccg
     8041 aatagaacgt gcctataagc atgagataga acgtcgtatg caaacactgg atttgttatg
     8101 ccatcgctta attgatcctc gggagtcgat caaacatcaa gttaatcgct tacagtattt
     8161 acgtgagaaa ctgttattct gttggagcag aaaaattgaa aaaatagatt ctgaaaatag
     8221 acatttgcaa cgaagattct tcagcagtgc acttaacctt gagcatatgc ttaatcagca
     8281 atttgaaaga tcttcaaaat tgcataaagc aataaattat tatctagaac gtcaagggac
     8341 gaagcttgtt cactatcaaa cccagctcac acatcttaat cctaaatctg tattacaacg
     8401 gggctacagc atcacttcta atgctgaagg taaaatttta cgggatagta gtcagatcaa
     8461 tatcgatgat tcaatccaag ttactttagc tgagggttgg gtaaaggcta aggtgagtga
     8521 tgccgggtaa tagaatcaat ggacttttca gttggggtag tgcagactga cgagctttat
     8581 aatcgatcgt ttattttaga aattattagg atcaagatgt cacgtttatt ccttgctctg
     8641 ggagcgatca atgctttcct ttgtgtacta ctgggtgcga ttggcgctca catccttaag
     8701 cagaagtttt ctggtgaact attcaatacg ttccagactg gcttgcaata tcatttttat
     8761 catgcattgg gtttgatttt gattggtgtc ttgttatttc acttacctca atcgaagtta
     8821 gtgaaactgt caggttgggt gatgctgacc gggatattgt ttttctctgg cacactttac
     8881 attgtcagct ttaccgaaat gcg
//
LOCUS       DAOQLJ010000091         8880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3089_length_8880_cov_6.185836, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000091 DAOQLJ010000000
VERSION     DAOQLJ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8880
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3089_length_8880_cov_6.185836"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..454
                     /locus_tag="PK522_07435"
     CDS             26..454
                     /locus_tag="PK522_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNC41603.1"
                     /translation="MSGCSTTTKTPTPSVSKSQSGAPYNRPYKVRGKTYYPMASAAGY
                     RAEGIASWYGSESGNKTAMGTRFNPQGLTAAHKTLPLPTKVKVTNLRNGRSVTVIVND
                     RGPFKKNRLIDLSHGAAKQIGLQGLTEVRIESINTVASNP"
     gene            complement(482..2581)
                     /gene="metG"
                     /locus_tag="PK522_07440"
     CDS             complement(482..2581)
                     /gene="metG"
                     /locus_tag="PK522_07440"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNC41604.1"
                     /translation="MSSRKLLVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMQGHTV
                     YYVCADDTHGTPIMLRAEKEGLTPEALIARVHGEHFSDFTGFNIRFDQYYSTHSAETR
                     HYSEDIYQKLKAAGLITVRAIEQLYDPVKQMFLPDRFVKGECPKCGAKDQYGDSCEVC
                     GAAYAPTELKNPYSAVSGATPIKKASEHFFFRLSDERCADFLRRWTREANHLQAEAAN
                     KMHEWLGQAGENKLSDWDISRDAPYFGFEIPGETAKYFYVWLDAPIGYMGSFKHFCTH
                     HGIDFDEYWKKESDTELYHFIGKDILYFHALFWPAMLENSGYRTPTQIFAHGFLTVNG
                     EKMSKSRGTFITAASYLQQQLNPEWLRYYYAAKLNGTLEDIDLNLEDFVARVNSDVVG
                     KFVNIASRCAGFITKRFEGRLVAGEDYRLLQQTIDTQFAAWQAGVIEQAYEARDFSTA
                     VRHIMKRADEVNELIHELAPWDIAKDATKTSELHRACSLGIQMFYLLSSYLKPILPAT
                     VEKIEQFLNCDSLKWPTQELGQPLSSLLLPAGHLIKPYQHLMTRIEPKQIEAMIVANK
                     TPADTQAPGAQSKAQPSDIAPIAETISIEDFSKIDLRIARIVDAQHVEGAEKLLKLTL
                     DIGIEQRTVFAGIKSAYDPEKLKGRLTVMVANLAPRKMKFGLSEGMVLAASDPNVTGG
                     LFILSPDEGAKPGMRVK"
     gene            2703..3791
                     /gene="apbC"
                     /locus_tag="PK522_07445"
     CDS             2703..3791
                     /gene="apbC"
                     /locus_tag="PK522_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HNC41605.1"
                     /translation="MALTEQHIEETLKTVIDQTTGKDYVSSKAVENIKIVDNHVSLDI
                     ALGYPAKTMLESIRQQISEALKSIAGVSTVTVNVFSKIIPHSVQRGVKLIPGVKNIIA
                     VASGKGGVGKSATAVNLALALAAEGANVGILDADIYGPSQPQMLGLTGHPESPDGKTI
                     EPMMAHGIQAMSIGLLIDAETPMVWRGPMVTQALQQLLNDTRWHDLDYLVIDLPPGTG
                     DIQLTLAQRVPVTGAVIVTTPQDIALLDARKGLKMFEKVGVPILGIVENMSMHICSKC
                     GHTEHIFGTGGGEKMCADYNVELLGALPLDIRIREHSDAGRPSIVAEPDGAIADAYRM
                     IARRVAAKIANLNKDYSDVFTQIIMEDN"
     gene            3799..4365
                     /gene="dcd"
                     /locus_tag="PK522_07450"
     CDS             3799..4365
                     /gene="dcd"
                     /locus_tag="PK522_07450"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNC41606.1"
                     /translation="MAIKSDKWIRKMAMEHGMIEPFEPGQVKQKEGQNIVSYGTSSYG
                     YDIRCSDEFKLFTNINSTIVDPKQFDSNSFVDVKSEVCIIPPNSFALARTVEYFRIPR
                     SVLTICLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYANEGVAQVIFF
                     ESDEICETSYKDRKGKYQFQQGVTLPKI"
     gene            complement(4396..5634)
                     /locus_tag="PK522_07455"
     CDS             complement(4396..5634)
                     /locus_tag="PK522_07455"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNC41607.1"
                     /translation="MKKPVKMLYEVINEKNHHLVKSWTKGVIFDENAKKQVLNIASMP
                     FIHKHIAIMPDVHLGKGATIGSVIPTIGAVIPAAVGVDLGCGMMAVKTSLKAIDLPDT
                     LSNLRHEIEKQVPHGRSKQIKKGRDKGSWSELPEDVIQAWLPLQHRFNRLCNQHPWLE
                     KTNHRNHLGTLGTGNHFIELCLDEKNQVWVMLHSGSRGVGNKIGSEFIEKAKKDMERW
                     LIHLPDKDLAYIPEGSAHFDDYINAVSWAQDFARINRTVMMQRTLAAMQKILEQPFTA
                     ELKAVNCHHNYISREYHFGQDVWVTRKGAVSAKLGEMGIIPGSMGAKSFIVRGLGNAE
                     SFCSCSHGAGRVMSREEAKRRVSLDEHVQATAGVECRKDESVIDETPSAYKDIEKVMA
                     AQADLVEIVHTLKQVLCVKG"
     gene            5797..7518
                     /locus_tag="PK522_07460"
     CDS             5797..7518
                     /locus_tag="PK522_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNC41608.1"
                     /translation="MKRQISTILAIAIFNFSVSTPLYALDLYVDTKTKQIFAEPGPGR
                     VHLGTYEKSGSSSAKAAPETSRKTEVTELAPTHENAEANRSYQPTGDRLERIREKASK
                     EQLKNQVSVLEERMKEVEKIHGTFDDRGLHWATKDGNFSVSLNGRIQPASQYNFINDP
                     DPAFGANTANELNSGMNIRRARLGVEGTFYKVWDYKFEYDFSRGNGTVGSGITDAFVR
                     LNHTDALSYKLGSFKEPFSLEEAASNRYLTFIERHMSVNAFVDNPNTYKTGIGANYAV
                     QRWQTGIAFQTEPIGSWSSAATSVNANGNQNRNNGSGDTGWQGIGRVSGRPWMEDDTK
                     FMHVGISAGHTEVNTQYRADGTMVGEGQIGGGGGMSFFAFPGTNVDRTNILNTGNLST
                     GALNDPNRRQVTSYDRYGAEGWFVYGPFSAQAEYLRTNINGVGYDGEHLTGYYGFISY
                     FLTGESKSYHVRNGAANRLKPRKPFHWDGSGLGAWEIAAGYDYIDMNSGAIKGGEMDM
                     FRFGLNWYPHSNVKFQSNIIHVFNINTAGTPITNTNGYSGGAGARTHGWNNGDLSAFL
                     TQITVDF"
     gene            7522..8646
                     /locus_tag="PK522_07465"
     CDS             7522..8646
                     /locus_tag="PK522_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC41609.1"
                     /translation="MFQMNTNWKHVLIMRMNHTLAARCSNAIALLEDKMLQLPHYLNR
                     FSFVVLLFVCSHLALVAHAQPTINIDGSSTVYPITKIVADQFQITKNNTVTTVVDISG
                     SGGGFKKFCRGEIDIVNASRPILRDEIQQCKNSGIQYIEIPIAYDALTVAINPANNWS
                     TRMTIAELKKMWEPAAQGNITKWKQINSAWPDEPISLYGADEDSGTYDYFTEAIIGRA
                     KSIRNDFTQSENDNHLVDAVASNKNAIGFFGFAYYIENQNRVTAVAINNGRGAILPST
                     DTVEDGSYQPLSRPVFIYVNIKSTDKAEIKEFVDFYLKNALLLVKEVKFFPLPPRAYK
                     TMLEHFNSKRVGTIFNGRPAIGLTIDELIRREGRLQYENY"
     gene            complement(8660..>8880)
                     /gene="trpC"
                     /locus_tag="PK522_07470"
     CDS             complement(8660..>8880)
                     /gene="trpC"
                     /locus_tag="PK522_07470"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181109.1"
                     /note="involved in tryptophan biosynthesis; amino acid
                     biosynthesis; converts
                     1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate to
                     C(1)-(3-indolyl)-glycerol 3-phosphate and carbon dioxide
                     and water; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HNC41610.1"
                     /translation="NRNLRTFETRLDTTLQLLDRIPSERIVITESGIKEKADIHLMRS
                     HQVNTFLVGETFMRADDPGIAMQQLFFA"
BASE COUNT         2478 a         2085 c         1896 g         2421 t
ORIGIN      
        1 gttgctcaca ttgattgtca gcttaatgag tgggtgttca acgacaacta aaacacctac
       61 gccatctgta tcgaaatcac aatcgggtgc gccctacaac cgaccttata aagtaagagg
      121 aaaaacttat tatccgatgg catcggctgc tggctatcgt gccgaaggca ttgcttcttg
      181 gtatggttca gagtcgggca ataaaaccgc gatgggtaca cggttcaatc cacaaggcct
      241 gacggcagcc cacaaaaccc taccactccc caccaaggta aaagtgacga atctgcgtaa
      301 tggtcgctca gtcactgtca ttgtcaatga ccgaggtcca tttaagaaaa atcgattaat
      361 cgatctatcg catggtgccg ccaaacaaat tggattgcag ggattaacgg aagttagaat
      421 tgaatcgatc aataccgttg ctagcaatcc ttgaaacagc caccggttgt atcatcaagg
      481 attacttgac tcgcatccca ggtttagcac cttcatcggg tgagagaata aacaacccgc
      541 ccgtaacatt tggatcgctc gctgcgagta ccattccttc ggacaaaccg aatttcattt
      601 tgcggggtgc taaattagcc accatgacgg ttagccgccc ctttaatttt tcaggatcgt
      661 aggctgattt aatgccagca aatacggtac gctgctcaat accgatatcc agcgtaagtt
      721 tcagtaattt ctctgcccct tccacatgtt gcgcatcaac aattcgggca atacgtaaat
      781 caatcttact gaagtcctca atcgagatgg tttctgcgat tggtgcgata tccgatggtt
      841 gggcttttga ttgcgcacca ggggcctgag tatcagcggg ggttttattc gctacaatca
      901 tcgcttcaat ttgttttggt tcgattcgtg tcatgagatg ttggtaaggt ttgattaagt
      961 gtcctgcagg tagtaataaa ctggacaaag gttgtcctaa ctcctgagtt ggccatttta
     1021 aggaatcaca attcaggaat tgttcaattt tttctacagt tgccggaaga atgggtttta
     1081 aataactcga aagcaggtaa aacatctgta tgccgaggct acaagcacga tgtagctcgc
     1141 tggtttttgt tgcatcttta gcgatatccc acggagccag ttcatgaatc aattcattga
     1201 cctcatccgc acgtttcata atatggcgaa ctgcggtcga gaaatcccgt gcctcataag
     1261 cctgctcgat gacgccagct tgccaggctg caaactgcgt atcgatcgtt tgctgcaata
     1321 accgataatc ttcacctgca actaatcgac cttcaaatcg tttagtaata aatccagcgc
     1381 agcgactcgc gatatttaca aatttaccga ccacatcgga attcacgcgt gcaacaaaat
     1441 cctctaaatt gagatcaata tcctctaatg tgccattaag ttttgcagca tagtaataac
     1501 gtaaccattc cggatttaat tgttgctgca aataactggc tgcggtaata aaggttccgc
     1561 gcgatttgct cattttttcg ccattcactg ttagaaaacc atgtgcaaaa atttgcgtgg
     1621 gggtgcggta cccggaattc tcaagcattg caggccaaaa cagtgcatgg aaataaagaa
     1681 tgtcctttcc aatgaagtgg tagagctcgg tatcactctc ttttttccaa tactcatcga
     1741 aatcaatccc atgatgagtg cagaagtgct taaaactacc catatagccg atgggcgcat
     1801 ctaaccaaac ataaaaatat ttggctgtct caccggggat ttcaaaacca aaatagggag
     1861 catcacgaga aatatcccaa tcggaaagct tattttcacc ggcttgcccc agccattcat
     1921 gcatcttgtt agctgcttcg gcttgtaaat gattcgcttc ccgcgtccag cgccgtaaaa
     1981 aatcggcaca acgttcatcg gacaaccgaa aaaagaaatg ctcagaagct tttttaatcg
     2041 gtgttgcgcc agaaaccgct gaatacggat tttttaattc agtcggtgca taggcggcac
     2101 cacatacttc gcaggaatcg ccatattgat ccttggcacc acatttgggg cattcccctt
     2161 tcacaaagcg atctggtaaa aacatctgct taaccggatc atacaactgc tcgatggcac
     2221 gtacggtgat taatcccgcc gcttttaatt tttgataaat atcctctgaa taatggcgtg
     2281 tttccgcaga atgtgtactg taatactgat cgaagcgaat attaaaaccc gtaaaatcac
     2341 taaaatgttc cccatgaaca cgcgcgatca acgcttcggg tgtcaatcct tctttctcag
     2401 cacgcaacat aattggcgtg ccatgcgtat catctgcaca aacataataa accgtatgcc
     2461 cctgcatttt ctgaaaacgc acccaaatat cggtttggat atattccaca agatgaccta
     2521 ggtggatact gccatttgca tagggtagtg ctgatgtaac gagaagtttg cgtgaactca
     2581 ttgatagact atctttgtta tattgtttat aaacgtaaat tgtaacaaac tggatagtta
     2641 ccctcgatat ttgttaacat cgaaatcttt cattttaatg atttatgcaa aaggagcacc
     2701 ctgtggcact aaccgaacaa cacattgagg aaaccctgaa aacagtcatt gatcagacta
     2761 ctggtaaaga ttatgtcagc agtaaggcgg tagagaacat taagatcgtt gataaccacg
     2821 tttcactcga tattgcactg gggtatcctg ccaaaaccat gctggaatcg attcgtcagc
     2881 aaatcagcga agcgttgaaa tcgattgcag gcgtcagtac ggttacggta aacgtattta
     2941 gcaaaattat tccacacagc gtgcaacgcg gcgttaagct gattccaggg gttaagaata
     3001 tcattgcggt tgcttctggc aaaggcgggg tgggaaaatc cgcaaccgcc gttaatcttg
     3061 cacttgcact cgcagccgaa ggtgctaacg tcggtattct cgatgcggat atttatggtc
     3121 catcccaacc ccagatgctg ggccttaccg gccaccctga atctccagat ggaaaaacca
     3181 ttgaaccaat gatggcacat ggtattcaag ccatgtcgat cggtctgtta atcgatgctg
     3241 aaaccccgat ggtctggcgc ggaccaatgg tgactcaagc tttgcagcaa ttactcaatg
     3301 atacgcgctg gcatgattta gattacctgg tcatcgattt accgcccgga actggagata
     3361 ttcaactcac cctagcgcaa cgcgttccag tcacgggtgc ggtcatcgtt actacaccgc
     3421 aggacattgc cttgcttgat gctcgcaaag gtttaaagat gtttgaaaaa gtgggcgttc
     3481 ccattctggg catcgtggaa aacatgagta tgcatatctg ctcaaaatgc ggccataccg
     3541 aacatatttt cggtacaggg ggcggtgaaa aaatgtgtgc tgattataat gtcgagcttc
     3601 tcggtgcgct gcccctcgat atcagaattc gcgaacactc agacgcagga aggccaagca
     3661 ttgttgcaga acccgatggc gcgattgcgg atgcgtatcg tatgattgcc cgtcgcgtgg
     3721 cggctaaaat tgccaacctt aacaaagatt attctgacgt atttactcaa atcatcatgg
     3781 aagataacta atttcactat ggcgatcaaa tcggacaaat ggattcgcaa aatggcaatg
     3841 gaacacggaa tgatcgagcc atttgagcca gggcaagtta agcaaaaaga aggccagaat
     3901 atcgtctctt atggaacatc gagctatggc tacgatatcc gatgttccga tgagttcaag
     3961 ctattcacca atataaactc cacgattgtc gatcctaaac agttcgactc caattccttt
     4021 gtcgatgtca aaagcgaggt ttgcatcatt ccccccaatt cgtttgccct tgcccgaacg
     4081 gtggaatatt tccgtatccc tcgcagcgtg ctgaccattt gcctcggaaa atcgacttat
     4141 gcgcgctgcg gtatcatcgt caatgtcacg ccgtttgaac ccgaatggga aggttatgta
     4201 acgctcgaat tttccaacac gacccccctc cccgccaaaa tttatgcaaa cgaaggggtt
     4261 gcgcaagtga ttttctttga atcagacgaa atttgcgaaa cctcgtataa agaccggaaa
     4321 ggtaaatatc aattccaaca aggtgtcaca ttacctaaaa tttgatctta cttatctttt
     4381 gatatttcta atcagttacc ctttaacaca taaaacctgt ttcagggtat gcacgatctc
     4441 gaccaaatcg gcttgggctg ccatgacttt ttcaatatct ttgtaggcac tcggcgtttc
     4501 atcgatcaca ctttcatctt tacggcattc cacaccggct gtggcttgca catgttcgtc
     4561 cagtgagacc cggcgctttg cttcttcgcg tgacatgaca cgccctgccc catggctgca
     4621 gctacaaaaa ctctcggcat tgcccaagcc ccgcacgata aatgacttcg cccccatact
     4681 accggggatt atccccattt cacctaattt agcggaaaca gcccctttgc gcgttaccca
     4741 cacatcttgg ccaaagtgat attcccggct gatgtagttg tgatggcaat tcactgcttt
     4801 taactcagcc gtaaagggtt gctccaatat tttctgcatc gccgctaaag ttcgctgcat
     4861 catcacggtt cgattgatgc gtgcaaaatc ttgtgcccac gatactgcgt taatatagtc
     4921 atcgaaatgc gcgctgccct ctggaatata ggctagatct ttatctggca aatgaataag
     4981 ccatcgctcc atgtcttttt ttgctttttc gataaattcg gaaccaatct tgttaccgac
     5041 cccccgtgaa ccactgtgca acatgaccca aacttgattc ttctcgtcta aacataattc
     5101 gatgaaatga ttgcctgttc ccaacgtacc caaatgattt ctatggttag ttttctcgag
     5161 ccagggatgc tgattacaca accgattaaa tcgatgctgc aagggtaacc aggcctggat
     5221 gacgtcttcg ggtaattcac tccaacttcc tttatctctc ccttttttaa tctgtttgga
     5281 acgtccatgc ggtacttgct tttcaatttc atggcgaaga ttactcagcg tatctggcaa
     5341 gtcgatggct tttagtgatg ttttaactgc catcatgcca caccccaggt caacacccac
     5401 cgctgctgga ataaccgccc ctatcgtagg aatgacactg ccaattgtgg cacctttgcc
     5461 taaatgtacg tcgggcatga tggcaatatg cttatggatg aatggcatcg aagcgatgtt
     5521 caagacttgt ttcttggcgt tttcatcaaa tatcacgcct ttcgtccaag actttactaa
     5581 atgatgattc ttctcattaa ttacttcgta cagcattttc accggttttt tcatgcgtaa
     5641 tttctaactc aaaacaagct catatttttc taccatttaa tcaaacagtt gcgttacaca
     5701 attatgaata ttttatgact gtaataattt tgtaacatgt gtagcttata aaggttgcca
     5761 gatttttaac tttattttct atcttggatt aaacccatga aaagacaaat tagcacgatt
     5821 ctggcaatcg cgattttcaa ttttagtgtt tccactccgc tttacgcact cgatttatac
     5881 gttgatacca aaaccaaaca aatttttgca gaacctggcc ctggtagggt tcatctagga
     5941 acttacgaaa aatctggaag ttcatctgca aaagctgctc cagaaacttc gagaaaaacg
     6001 gaagtgacgg aattggcccc tactcatgag aatgcagaag caaacaggag ttatcagcct
     6061 acgggtgacc gccttgaaag aatacgggaa aaagcttcca aggagcagct taaaaatcaa
     6121 gtctcagtcc tcgaagaacg catgaaagaa gtcgaaaaaa ttcatggtac gtttgacgat
     6181 cgcggtctgc attgggcaac taaagatggc aacttctcgg tctcactgaa tggacggata
     6241 caaccggctt ctcaatacaa tttcatcaat gaccctgacc cagcttttgg ggcgaatacc
     6301 gcaaacgagt tgaatagtgg catgaatatt cgccgtgccc gtttaggggt agagggtacc
     6361 ttctataaag tatgggacta caaatttgaa tatgacttca gccgtggtaa tggtaccgtt
     6421 ggttctggca ttaccgatgc cttcgttcgc ttaaaccaca ccgatgcact ttcttacaag
     6481 ctgggttcat tcaaagaacc gttcagtctg gaagaagcgg caagcaaccg ttacctcacc
     6541 ttcatcgaac ggcatatgtc ggtcaacgct tttgtcgata accccaatac ttataaaacc
     6601 ggtatcggtg caaactatgc ggtgcaacgc tggcaaacgg gtattgcttt ccaaactgaa
     6661 ccgattggtt catggtcatc tgctgcgacc tcggtcaatg ccaatggtaa ccaaaatcgc
     6721 aataacggtt ctggcgacac cggttggcaa ggaatcgggc gtgtttccgg aagaccttgg
     6781 atggaagacg acacgaaatt catgcatgtc ggtatttcag caggtcatac cgaagtcaat
     6841 acccaatacc gtgcggatgg caccatggtc ggtgaaggtc aaattggcgg cggcggtggc
     6901 atgtcattct tcgccttccc cggcaccaac gtcgatcgta ccaatattct caacaccggt
     6961 aacttaagca cgggggcatt aaatgatcct aaccgccgtc aagtcacgag ctatgatcgt
     7021 tacggtgctg aaggctggtt tgtatatgga cctttctcag ctcaagctga gtatttacga
     7081 accaacatca atggagtggg atacgatggc gaacacctta cgggttacta tggatttatc
     7141 agctatttcc tgactggcga atcaaaatcc taccatgttc gaaacggtgc agcgaatcga
     7201 cttaaaccgc gtaaaccctt ccattgggat ggttcaggtc tgggtgcttg ggagattgct
     7261 gccggctatg actatatcga tatgaattcc ggcgcgatca aaggtggtga aatggatatg
     7321 ttccgatttg ggttaaattg gtatccgcac tccaatgtta aattccaaag caacatcatt
     7381 catgtgttca atatcaatac cgctggcaca ccgattacta ataccaatgg ttattcgggt
     7441 ggagccggtg cgcgcactca cggttggaat aatggcgacc tcagcgcatt tctcacacaa
     7501 attactgtag atttctaaat catgtttcaa atgaatacaa actggaaaca tgttttaatt
     7561 atgagaatga accatacact tgccgctcgt tgcagcaatg caatcgcgct attggaggat
     7621 aaaatgttgc agttaccgca ttatcttaac cgctttagtt ttgttgtttt attgtttgtt
     7681 tgcagtcatc tagcgctagt cgctcatgct caaccgacca ttaacatcga tgggtcgagt
     7741 acggtctatc ccatcactaa aatcgttgct gatcaatttc agatcacaaa aaataatacg
     7801 gtaacgacag ttgttgatat ctcaggcagc gggggtggtt tcaaaaaatt ctgccgtggt
     7861 gagatcgaca tcgttaatgc ttctcggccg atactccgcg atgaaattca gcaatgtaaa
     7921 aattcaggta ttcaatatat cgaaatcccg attgcttacg atgccttgac cgtcgcgatc
     7981 aatcccgcta ataactggag cactcggatg acaatagctg agctcaaaaa aatgtgggaa
     8041 cctgcggcac aaggtaacat caccaagtgg aaacaaatta actcagcttg gcctgacgaa
     8101 ccaatcagtc tttatggggc tgatgaagat tcaggaacat acgattactt taccgaggcg
     8161 attatcggac gcgccaaatc aatccgtaat gatttcactc aatcggaaaa cgataatcac
     8221 ctagtcgatg ccgtcgcgag caataaaaat gcaattggtt tctttgggtt tgcctactat
     8281 attgaaaacc agaatcgagt cactgctgtt gcaatcaata acggtagagg cgcgattctc
     8341 ccttccaccg ataccgtcga agatggaagc tatcaacctt tgtcacggcc tgtttttatt
     8401 tatgtgaata ttaaatcaac cgacaaagcg gaaattaaag aattcgtcga tttctattta
     8461 aagaatgcac tgttgctcgt caaagaagtt aagttctttc cgctaccacc gagggcttat
     8521 aaaaccatgt tagaacactt caatagcaaa cgcgtgggta ccatcttcaa cggaagaccg
     8581 gcaattggtc tcaccatcga tgaattgatt cgtcgtgaag gaagattgca atacgagaac
     8641 tactaattta acgcatccct caagcaaaaa agagttgctg cattgcaata ccaggatcat
     8701 cagcacgcat aaacgtttct cccactaaaa aggtattcac ttgatgcgat cgcatcaagt
     8761 gaatatccgc tttctctttt atcccactct ctgtaatgac gatgcgctcc gaaggaatac
     8821 gatctagtaa ttgcaacgta gtatcgagac gcgtttcaaa agtacgcaaa ttacggttgt
//
LOCUS       DAOQLJ010000092         8879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3090_length_8879_cov_5.300997, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000092 DAOQLJ010000000
VERSION     DAOQLJ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8879
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3090_length_8879_cov_5.300997"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /gene="ycaC"
                     /locus_tag="PK522_07475"
     CDS             complement(<1..575)
                     /gene="ycaC"
                     /locus_tag="PK522_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HNC41611.1"
                     /translation="MNKTFKYNRLSKDDAVLLLVDHQAGLISLVQDFSPNEFKNNILA
                     LTACGKYFKLPTILTTSFEDGPNGPLVPEIKEMHPAAPYIARPGNINAWDNKDFVNAV
                     KKTDRKQLIIAGVVTEVCVAFPALSAIEEGYEVFVVTDASGTFNEVTRDAAWARMQSA
                     GVQLMNWFGVACELHRDWRNDVEGLGALFSS"
     gene            complement(614..964)
                     /locus_tag="PK522_07480"
     CDS             complement(614..964)
                     /locus_tag="PK522_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNC41612.1"
                     /translation="MTLEEKFNDFNQMILDGEILESMDKYYHSDCIVIEKNDVVATGL
                     QQAKARESELFEGVEAWLKSEIVSTAVGENVTMTEWHYEYKHKDFGHKKFDQVAVQHW
                     KNGKIISDRFYALY"
     gene            complement(981..1613)
                     /locus_tag="PK522_07485"
     CDS             complement(981..1613)
                     /locus_tag="PK522_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNC41613.1"
                     /translation="MSHINPDETLMLLIDHQSGLFQLVKDIPVSDLRRNAIALAKVAT
                     ILKMPVITSASVPDGPNGPLIPEIHQAAPHAQYVGRRGEINVWDSPDFVKAVEATGRK
                     TLILAGTLTSVCMAFPSISAVAAGYRVYAVVDASGNWSQMGTELTIARIVQAGVIPID
                     TVAVISELQKTWNRPEAIEFADLYADYPMLHYRLLIESYDKAQTVQKAKN"
     gene            complement(1704..2594)
                     /locus_tag="PK522_07490"
     CDS             complement(1704..2594)
                     /locus_tag="PK522_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNC41614.1"
                     /translation="MQIIQSEASIKKLLRIQCNAHQHWVGDGFPVRTLFSYSALGPMI
                     SPFLLFDYAGPMEFQATTQRRGIGEHPHRGFETVTIVYSGEVEHRDSSGGGGKIGPGD
                     VQWMTAASGLVHEEFHSRDFARRGGALEMVQLWVNLPAKNKMSPPRYQSILDSQIPAI
                     DLPQDQGKIRVIAGEFGGLTGPARTFTPINVWDLRLSNGQLIDLAVPDGYNTLLAVLK
                     GSMRVNNSETINTAEVGLFDQTGDHIRITSTSDTTALLLCGKPIDEPIVGSGPFVMNT
                     TEEIRQAMADYQSGKMGKIT"
     gene            2698..3039
                     /locus_tag="PK522_07495"
     CDS             2698..3039
                     /locus_tag="PK522_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC41615.1"
                     /translation="MPSKEVYCPVEVTLKVIGGRWKVLIIHQLLNGTMRFNELQRNLI
                     GITHRTLSRQLREMEMHQLIIRNDFNEIPPRVEYSLSPLGYSLKNILFAMDEWGRNII
                     CKNSQGCLQRI"
     gene            3424..3843
                     /locus_tag="PK522_07500"
     CDS             3424..3843
                     /locus_tag="PK522_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNC41616.1"
                     /translation="MQLKGSKTEGNLKAAFAGESQANRRYLYFAAKADVEGQNDVAAV
                     FRSTAEGETGHAHGHLEYLESCGDPATGLPFGSSRDNLKTAIAGETHEYTDMYPGMAK
                     TARDEGFTEIADWFETLAKAERSHANRFQRALDGFTD"
     gene            4467..4859
                     /locus_tag="PK522_07505"
                     /pseudo
     CDS             4467..4859
                     /locus_tag="PK522_07505"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
     gene            5394..5984
                     /locus_tag="PK522_07510"
     CDS             5394..5984
                     /locus_tag="PK522_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HNC41617.1"
                     /translation="MAKISRESLMTLEAYAKTRQQFRTQVIAHKKDRLIALGEHITLL
                     FEDELTIRYQIQEMLRAEKIFDEEGILQELAAYAPLVPDGTNWKVTMMIEYADPKERA
                     ERLAQLIGIEDKVWIEVEGYEKILAIADEDLDRENEVKTSSVHFLRFELSHEQIQALH
                     RGSTLRIGVSHPYYEAITEAIKNPVRAALLNDLNLP"
     gene            5981..6493
                     /locus_tag="PK522_07515"
     CDS             5981..6493
                     /locus_tag="PK522_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HNC41618.1"
                     /translation="MKLRLVFLSLVSLVSCSSNEKFVDTFDNSKNWVEIQAQLPNYPE
                     TENLLEFDAGPANDMRHYVDAQSIAIGEDSVIRFTLVSKSSEGAENISFEGIRCRTRE
                     RKRYAVGRLNKTWAHSRQPEWQLLNNVGQNHALRELAKFYFCPQDIMVGSQQEAIQAL
                     KAGIHPRVVR"
     gene            complement(6507..7115)
                     /locus_tag="PK522_07520"
     CDS             complement(6507..7115)
                     /locus_tag="PK522_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNC41619.1"
                     /translation="MNKSDVEILEKKLCYQGFFRMERYALRHRLFNGTWSKPIVRELM
                     ERGHAAAVLPYDPVSDCVVMIEQFRIGAVSAPVGPWLLEIVAGIIERDEPAEQVVKRE
                     AVEEAGCTIHELIPLFDYLVSPGGTSERIALYCGRVEANHIVGGFYGIEDEGEDIKVH
                     VMPFAEALSLLNTGKIAAASAIIALQWLALNRDSVRNRWLTA"
     gene            complement(7167..8288)
                     /gene="proB"
                     /locus_tag="PK522_07525"
     CDS             complement(7167..8288)
                     /gene="proB"
                     /locus_tag="PK522_07525"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNC41620.1"
                     /translation="MNQAVLSAARRIVIKVGSSLVTNQGQGLDHQALAGWAKQISALK
                     QLGKEIILVSSGAIAEGMQRLSWKKRPTAIYELQAAAAVGQMGLAQAYADCFSQYDLL
                     TAQILLTHDDLSNRKRYLNARSTLSTLLKLNIIPVINENDTVVIDEIRFGDNDTLAAL
                     VTNLVEAEVLVILTDQSGLFTSDPRKNDNATLVQEANAGDPEIEAMAGGAGSEIGRGG
                     MQSKVIAAKRAARSGAHTVIASGHEREVLVRLAKGEAIGTCLLANTPVLIARKQWLAD
                     HLQIRGSVILDSGAIKALVEEGKSLLPIGVIDVKGEFDRGEAVSCMDKDGNELARGLI
                     NYSARETRKIMRQPSQNIEELLGYLDEYELIHRNNLVVL"
     gene            complement(8291..>8879)
                     /locus_tag="PK522_07530"
     CDS             complement(8291..>8879)
                     /locus_tag="PK522_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNC41621.1"
                     /translation="DEFQLKLELKVLADVGLLGMPNAGKSTLIRAVSAARPKVADYPF
                     TTLHPNLGMVRVDHHRSFVMADIPGLVEGAADGVGLGHHFLKHLERTRLLLHVVDIAP
                     MNDTIDPVQEAKALVEELRKYNEALYHKPRWIVFNKIDLLPASEQEAACEQFIRDLKW
                     KDKWFSISALTGEGCKSLTYAVMDYLEQTFSAMQH"
BASE COUNT         2532 a         2066 c         1813 g         2468 t
ORIGIN      
        1 taactagaga ataaggcacc taagccttca acatcgttgc gccaatctcg atgcaattcg
       61 caagccacgc caaaccagtt catcaattgt acgccggccg attgcatacg cgcccaggct
      121 gcatcgcgtg ttacctcatt gaaagtaccg gaggcatcgg taactacgaa gacttcataa
      181 ccttcttcga ttgcggatag tgccgggaaa gcgacacata cttcggtaac cacgccggca
      241 atgattaatt gtttgcggtc ggtctttttg actgcgttaa caaagtcttt gttatcccag
      301 gcattgatat tgccggggcg tgcaatataa ggcgcagccg gatgcatttc cttgatttcc
      361 ggaaccagcg gaccattggg accatcttcg aaactagttg ttagaatggt tggcaacttg
      421 aaatacttgc cacaagctgt cagagccaat atattgtttt tgaactcatt cggtgaaaaa
      481 tcctgcacca gagaaatcaa tcctgcttga tggtctacca gtaataaaac cgcatcatct
      541 tttgaaagcc ggttatattt aaaagttttg ttcattattt ttctccttat gctactgatt
      601 gtaaattcat cgctcagtac aacgcataaa agcgatcact gatgatcttg ccattcttcc
      661 agtgctgtac cgctacttga tcgaatttct tgtgaccaaa gtctttatgc ttgtattcgt
      721 aatgccactc tgtcattgtc acgttctcac ctaccgcagt cgatacaatt tccgatttta
      781 gccatgcctc gacgccttca aatagctcgg attcgcgcgc tttagcttgt tgcagacccg
      841 ttgctaccac atcatttttt tcgatcacga tgcaatcaga atggtaatac ttatccattg
      901 attccaatat ttcaccatct agaatcatct gattaaagtc attgaatttt tcttctaaag
      961 tcattttaat tctctcaaac ttaattcttc gctttctgca ccgtctgtgc cttgtcatag
     1021 ctttcgatca acagccggta atgcaacata ggataatcag cgtagagatc agcaaattct
     1081 attgcttctg gccgattcca ggttttttgc aactccgaaa taactgcaac agtatcgatc
     1141 ggaatcactc ccgcttgcac aatgcgagca atggttaatt ctgtgcccat ttggctccaa
     1201 tttcctgacg catctactac agcatatact ctataaccag cagccactgc gctgatggaa
     1261 ggaaaggcca tgcatacgct ggtcagcgta ccagcaagga tcagtgtttt gcggccagta
     1321 gcttcaactg ctttgacaaa atcagggcta tcccaaacat tgatttcccc gcgccttccc
     1381 acgtactgcg catgtggagc ggcttgatgg atttccggaa tcagtggtcc gttcgggcca
     1441 tccggaacag atgccgaagt aattaccggc attttgagta ttgtagcaac tttggcaagt
     1501 gcaatggcat tgcggcgcaa gtctgacact ggaatatcct tcaccagttg aaatagaccg
     1561 ctttggtgat cgataagaag catcagggtt tcgtcgggat tgatgtgact catggtattc
     1621 tcctatgtta gttttgctaa aaaatggtta cttaatagtc aaaccaattt gtgtttacaa
     1681 gtagtttttg ctatacatgc tatttatgta atttttccca ttttcccgct ctggtaatct
     1741 gccatggctt ggcgtatttc ctctgtagta ttcatgacaa atgggccgct accgacaatg
     1801 ggttcatcaa tcggtttacc acacagcaac aacgctgtcg tatcggatgt actagtgatg
     1861 cgaatatggt ctcccgtttg atcaaacagg ccgacctccg ctgtattgat agtttcagaa
     1921 ttattaacgc gcatcgaacc tttgagtacc gccagcaatg tgttataacc atcaggcacg
     1981 gcaaggtcga tcagctgccc attggataag cgcaggtccc agacgttgat gggtgtaaag
     2041 gtacgtgctg ggccggtaag accaccgaat tcacctgcaa tgacacgtat ttttccttga
     2101 tcttgtggca aatcgatggc ggggatctgg ctatccagaa tactttgata acgtggcggt
     2161 gacatcttat tcttggcggg caaatttacc cataactgaa ccatctccag cgcaccgccg
     2221 cgccgtgcaa aatcacggct atgaaattct tcatggacca gaccggaagc tgctgtcatc
     2281 cattgcacat cgccagggcc gatctttccg ccgccaccgg aactatcacg atgttcaact
     2341 tcgccgctat acacgatagt gactgtttca aagccacgat gaggatgctc accaataccg
     2401 cgacgttggg tagttgcttg aaattccatt gggcccgcat aatcaaacag caaaaaggga
     2461 ctgatcattg gccccaatgc agagtaagaa aatagtgtgc gtaccgggaa accatcacca
     2521 acccaatgct gatgagcatt acactgaata cgtaacaatt ttttaatgga tgcttcagat
     2581 tgaattatct gcatgactga ttaccttccg acaaaattat atttgataga gtaatatcat
     2641 tggaattaat tacaagtacg cacaaaaaag ataccgtcaa aatcaggaaa actcaaaatg
     2701 ccaagtaaag aagtttattg cccggtagaa gttacactaa aagtaattgg aggtcgctgg
     2761 aaggttttga ttatccatca actattaaat ggtacgatgc gatttaacga actgcagcga
     2821 aatttaatcg gtattactca tcgcacctta agccggcagt tgcgcgagat ggaaatgcat
     2881 caattaatta tcagaaatga cttcaacgaa attcctccac gcgtcgaata ttcgttatca
     2941 cctttaggct attcacttaa gaatattctc tttgcaatgg atgaatgggg gagaaatata
     3001 atatgtaaga attctcaggg ttgtttacaa aggatttgat taaatttagt ctaataaaca
     3061 accccatgcc gaaaccacgg ggaatcgcaa gttaataaaa aagcatagcg taatttccag
     3121 aaattatctg gtctgatctc attaattggc aagcaacaaa tagagaaaat ctggatttgt
     3181 agcaaataag ctgctgcatt gaaacacaaa gtattgtata gattctagag gttctagact
     3241 cataaatact ctacactctg tagttgacat caatcagtct gttcgatagt ctcatgcgaa
     3301 ggatagctag aatcctatta tcatgatcag agatgatttc gattcatctc ttttgcctac
     3361 gagattgcaa atcatagttt gtttgtctat tttgattaac tgcattgcaa aaaggagaga
     3421 gttatgcaat taaaaggatc aaaaaccgag ggaaatttaa aagctgcgtt tgcgggtgaa
     3481 tcccaagcta atcgtcgcta tctttatttt gctgccaaag ctgatgtcga agggcaaaat
     3541 gatgtggctg cggtattccg ctctacggcc gaaggtgaaa caggccatgc tcatggtcat
     3601 ctagagtacc ttgaatcctg tggtgatcca gcaacaggtc tgccatttgg ttcctcacgc
     3661 gataatctca aaacggcgat tgcgggtgaa actcacgaat ataccgacat gtatcctgga
     3721 atggccaaaa cggcacgcga tgaaggtttt accgaaattg cggattggtt tgaaacactt
     3781 gccaaagctg aacgttccca tgcaaaccgt ttccaacggg cgttggatgg ttttactgat
     3841 taaccatttt gatcgttaat cgacagtagt ctatttcaaa gcaagccaag caaaagcctt
     3901 tgcttggctt taagaatgga atctagtcaa tatataaata ttgataaact cggtaatact
     3961 ttatttaccg gataatttgt ctaattagtt gtaatttttc tgctatgact actcgcgaag
     4021 gcagcctcga agcccctaaa cgccatcccc tcaattggaa gcaaactgat ttttataatg
     4081 aagatcggct ctttgaggaa atgaatcgcg tttttgatat ttgccatgga tgccgacgct
     4141 gcgttaattt atgtaccgca ttcccaaagt tatttgattt aatcgatgat gggaaaaccg
     4201 gtgaactaga cggggtagaa aaaaaaacct tctgggaagt agtcgatcgc tgctatttat
     4261 gcgatatgtg cttcatgaca aaatgccctt atgtccctcc tcacccatgg aacattgatt
     4321 ttccccattt aatgctgcgc gctaaggctg ttaaatataa aaaacaaggt gcgccattcc
     4381 gtgacaagtt attagcgaat accgataccg ttggaaaatt agcaaccatt cctgtcgttg
     4441 ttcaggtaac caatacgctc accaaaatgc cggtagccag aaaattattg gaaaacacgt
     4501 tgggtattca tgccgacagg aaattacccg attacgcgcc acaaaaattt cgtaaccacg
     4561 cacaacccaa ttctgatttc attgaaaaag aaggagcgag aactccgggt aaagttgcca
     4621 tctttgcaac ctgttatatc aactacaacg aacccaatat tgggcatgac ttactgcaaa
     4681 tattagcgca taacgaaata cctacttgtt tggttgaaca agaagtctgt tgtggtatgc
     4741 ctcagctaga gttaggcaat ttagagaaag tcgaagaata taaaaataag aatatcccag
     4801 ttctcgccaa acttgctcaa gcgggttatg ccattcttac tgccgtacct tcttgtacgc
     4861 tgatgttcaa gcaagaatta ccactgatgt tccctgacga tgaagcggtt caagcagtcg
     4921 ctgcggctat gttcgatcct ttcgaatact ttatgttgcg tcatcaggat caactcctga
     4981 aaacggattt tacccgttca ctcggaaaaa tttcttacca cattccttgc cacttacgcg
     5041 tgcaaaatat cggtaagaaa accagagacc ttctacagct cattccggat actcacgtaa
     5101 caaccattga acgttgctcc ggtcatgatg gtacatgggg ggtcaaacgt gaatttttct
     5161 cagactccat gaaaatcggt aaacctgttt ttaaccaaat ggcggcgccc gatcctgatt
     5221 atatctgctc cgattgcgct attgcagcac gacatataca gcagggaatt ggtcatcatc
     5281 gtgcgcaaaa acttcatccg ttgacactgt tgcgcctcgc ttacggtgaa aataaacctt
     5341 cattatctga accatcgatg gttgcccaac catcccacga aaacaaaaat tctatggcaa
     5401 aaatctcacg cgaaagcctc atgacactgg aagcctatgc caaaactcgg caacaatttc
     5461 gcactcaggt cattgcgcat aaaaaggatc gattgatagc actcggtgag catataacgc
     5521 tattatttga agatgagctc accattcgct atcaaatcca ggaaatgctt cgtgcagaga
     5581 aaatatttga tgaggaagga atcttgcaag aactagcggc ttatgctccc ttggttcctg
     5641 atggtaccaa ttggaaagta accatgatga tcgagtacgc tgacccaaaa gagcgggcag
     5701 aaaggctagc gcaacttatt ggaattgaag ataaggtctg gattgaagta gaaggatatg
     5761 aaaaaattct tgcgattgcc gacgaagatt tagatcggga aaatgaagta aaaacatcgt
     5821 cggttcactt ccttcgcttt gaattgagtc atgagcaaat tcaggcatta catcgtgggt
     5881 ctacattgcg tatcggcgtg agtcaccctt attatgaagc aatcactgaa gctatcaaaa
     5941 atcctgtccg tgcagcttta ctcaatgacc taaatttgcc atgaaattaa ggttagtatt
     6001 tctaagtttg gtcagcctag tttcttgctc aagtaatgag aaattcgtcg acaccttcga
     6061 taacagtaaa aattgggttg agattcaggc ccaattacct aattatcctg aaacagagaa
     6121 tttactcgaa ttcgatgcag gccctgccaa tgatatgcga cattatgttg atgcgcagtc
     6181 tattgcgatt ggagaagata gtgtcattcg ctttacgctc gtttcaaaat cttcagaagg
     6241 tgcagaaaat atcagctttg agggaattcg ttgtagaaca cgagaaagaa aacgctatgc
     6301 ggtaggtcgc ctgaataaaa catgggcgca ttcacgacaa cctgaatggc agttactcaa
     6361 taatgtcggg cagaaccatg cactacgtga gttagccaaa ttttattttt gtccacaaga
     6421 tatcatggta ggtagccaac aagaagcgat tcaggcactc aaggccggta ttcacccgcg
     6481 tgttgtaagg tgatagtttc tgaattctat gctgtaagcc agcggttacg aacggaatct
     6541 cgattcagtg ctagccattg aagtgcaatg atggcgcttg ctgccgcaat ttttccagta
     6601 ttcaataatg ataatgcttc agcaaaaggc attacatgaa ccttgatatc ttccccttca
     6661 tcctcgatac cataaaaacc accaacgata tgatttgcct cgactcgacc gcaatataac
     6721 gcgattcgct ctgatgtacc gcctgggctc accagataat caaagagcgg aatcaactca
     6781 tgaatcgtac aaccggcttc ttctactgct tctcgcttca caacttgttc tgctggttca
     6841 tcccgttcaa taattcctgc aacaatctcc agcaaccagg gtccgaccgg tgcagacaca
     6901 gcaccaattc taaactgctc aatcataacg acacaatcgc tcacaggatc atagggaaga
     6961 acagcagccg catgtcctcg ctccatcagt tcacgaacaa tcggtttact ccatgttcca
     7021 ttaaacaatc gatgacgcag cgcgtaacgc tccattctga aaaaaccttg gtaacacaat
     7081 tttttctcaa ggatttcgac atccgattta ttcatggatc aattctttcc aaacaattga
     7141 agattaggag aagcttatca aaaagattag agaaccacta aattgttgcg atggataagt
     7201 tcgtattcat ctagataacc gagtagttct tcaatatttt ggctgggttg tcgcatgatt
     7261 ttgcgtgttt ctctcgcact ataattaatt aaaccgcgcg ctagttcgtt cccatcttta
     7321 tccatgcatg aaactgcctc tccccgatcg aattctccct ttacatcgat cacaccaatg
     7381 ggtaataagc tcttaccctc ttcaactagg gccttaattg cgccgctatc tagaatcaca
     7441 cttccgcgaa tctgaagatg gtctgccaac cattgtttcc gggcaattaa cacgggtgta
     7501 ttagctagca aacaggttcc aatcgcttca ccttttgcta aacgaactag aacctcacgc
     7561 tcgtgccccg atgcaatcac tgtatgcgcc ccacttctgg cagcccgttt agcggcaatg
     7621 actttactct gcattccacc tcgcccgatt tcactccctg caccaccggc cattgcctcg
     7681 atttcaggat ctcccgcatt ggcctcctga acaagggttg cgttatcatt cttgcgaggg
     7741 tcactggtaa ataagcccga ttgatcggtc aaaataacca gaacctctgc ctctacgaga
     7801 ttggttacca atgcagccag cgtatcgtta tcgccaaatc gtatttcatc gatgaccacg
     7861 gtatcgttct cattgatgac tggaataata tttaacttta atagcgtgct cagtgtagac
     7921 cgggcattaa gatagcgttt ccggttcgat aaatcatcat gtgtcaataa tatttgcgct
     7981 gtaagcaaat cgtattgact aaaacagtct gcgtacgcct gcgccagacc catttgacca
     8041 accgccgctg cggcctgcag ctcatagatt gcggtaggac gtttcttcca actgagtctt
     8101 tgcattccct ctgcaatggc acccgaagaa accagaatga tttctttccc taactgcttt
     8161 agcgcagaaa tttgctttgc ccaacctgca agggcttgat gatcgagtcc ttgcccctga
     8221 ttcgttacta aactacttcc cactttgatc acaatgcgac gtgcagcgct aagtacagct
     8281 tgattcatga tcagtgttgc attgcactaa atgtttgttc caaataatcc atgacagcat
     8341 aagtcaagga cttgcacccc tcccctgtta aggctgaaat actgaaccat ttatctttcc
     8401 atttcagatc acgaatgaat tgctcacaag ccgcttcttg ttccgaggct ggcaataagt
     8461 caattttatt aaacacgatc cagcgaggct tatgatataa agcttcatta tatttcctga
     8521 gttcctcgac tagcgctttc gcctcttgaa caggatcgat cgtatcattc atcggtgcga
     8581 tatcgaccac atgaagaagc agtcgggttc gttctaaatg tttaagaaaa tgatggccaa
     8641 gccctacccc atcagcagcg ccctcaacca aacctggaat atcagccatg acaaagctac
     8701 gatgatgatc gaccctgacc atgcccaaat taggatgcag cgtcgtaaaa ggatagtcag
     8761 caacttttgg tcgagctgcc gacacagcac gaatcaaagt agatttaccc gcatttggca
     8821 ttcccaataa acccacatcg gcaagtacct tcaactcaag cttaagctga aattcatcc
//
LOCUS       DAOQLJ010000093         8877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3092_length_8877_cov_7.667649, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000093 DAOQLJ010000000
VERSION     DAOQLJ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8877
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3092_length_8877_cov_7.667649"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1481)
                     /locus_tag="PK522_07535"
                     /pseudo
     CDS             complement(1..1481)
                     /locus_tag="PK522_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918516.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
     gene            complement(1561..2343)
                     /locus_tag="PK522_07540"
     CDS             complement(1561..2343)
                     /locus_tag="PK522_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HNC41622.1"
                     /translation="MTTYNTPDRPDFIRQQYAFAAHIRNPKQFPCPEGIEARRMAVYR
                     ELFYNNVEAFIANTYPVLHNIMTEDRWHAMVQDYFANHLAQTPLFPEMPREFLKYLEH
                     ERQPQPDDPPFLLELAHYEWVELALSLFDEKIDNNAINLTGDLLEGIPVISPLAWVLS
                     YRFPVHKISPTFQPQQADEANPTHLIVFRDAEFDIHFIETNAITVHLLQRITHNTNQS
                     GQTILQRIAVEMNHPQPDMIVTYGEQLMQQLKEQGILLGTVR"
     gene            complement(2312..3178)
                     /locus_tag="PK522_07545"
     CDS             complement(2312..3178)
                     /locus_tag="PK522_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNC41623.1"
                     /translation="MNSFKYPVHGVGLGLRRVMLSTLADQNTESVNFWEVAPENWINV
                     AGRHGKQFRELTEKHPVICHGLSLSIGSPAPLDETFLQRLKQFLKEHRIRYYSEHLSY
                     CSDDGHLYDLMPIPFTEEAVRYVAERIRHVQDMLDQRIAMENISYYAAPGKQMEEIDF
                     LNAVLEEADCDLLLDVNNIYVNSINHRYDAEAFLRQLPAERIRYIHVAGHYQEAEDLL
                     VDTHGSSVIDPVWQLLEKAYQHFGVLPTLLERDFNIPALSELLEEAKIINHLQAKWLN
                     CACDHIQHARPS"
     gene            complement(3191..3535)
                     /locus_tag="PK522_07550"
     CDS             complement(3191..3535)
                     /locus_tag="PK522_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41624.1"
                     /translation="MSKKIIKPVSIALGTALLTTLSASNFAAESNPFAMKTLSSGYMQ
                     LASADDAKKGEQDSQPSTDQKKADHEGKCGEGKCGAKGEMKKNMEGQCGGKKAEQEGK
                     CGEGKCGSSNKK"
     gene            complement(3788..4117)
                     /locus_tag="PK522_07555"
     CDS             complement(3788..4117)
                     /locus_tag="PK522_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC41625.1"
                     /translation="MKNYFIAAVTGAIFVISGNAIAADIEAGKRKAAEVCASCHGADG
                     NSPAPNFPKIGGQPRTYLEKALKDYKTGDRKDPVMAGMAAALTPDDIGNLAFYYSSQN
                     IGLTMKK"
     gene            complement(4147..4479)
                     /locus_tag="PK522_07560"
     CDS             complement(4147..4479)
                     /locus_tag="PK522_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC41626.1"
                     /translation="MHKVKKLAVKLAFAVGLALAISGGVHAASDAEGVKSKTSMCEGC
                     HGIEGYRTAYPVAYHVPKLGGQHAVYLAKALRDYKAGERNHPTMKAIAATLSDKDIEE
                     LAAYYAGN"
     gene            complement(4579..6495)
                     /locus_tag="PK522_07565"
     CDS             complement(4579..6495)
                     /locus_tag="PK522_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC41627.1"
                     /translation="MPLITLEDSCLAFGHHALLNHVDLQLDPGERIGLIGRNGSGKSS
                     LLKVLAKEIKLDDGKLWHKPELKLVYVSQEPEFGTAGTVYHEVSRGLGHLSQVLNEYH
                     EISQALSEGKGSNETNFSRLASLQNFLELNDGWRIQTRVETVIHQLGLSAQDLIGNLS
                     GGTRKRVALARALVVLPDVLLLDEPTNHLDFTSIEWLEELLQTFAGSVIFITHDRRFL
                     DNVSTRIIELDRGLLGSFTGNFAHYLQKKMEMLQSEATQQHKFDKVLAQEEVWIRKGV
                     QARCTRNEGRVRRLEALRLERAARRTRVGSVNFNVEVGERSGHLVAELQQVTKCFGDK
                     TVIKDFSCRILRGDRIGLLGPNGSGKSTLLKLILGELEPDLGEIKRGTKLSIAYFDQM
                     RGQLNPESTVIEAINQGSDFIEINGERKHVISYLEDFLFAPQRARSPVKSLSGGERNR
                     LLLARLFTQPANLLVLDEPTNDLDIETLELLEVLLQSYTGTLFLVSHDRTFLDNVVTQ
                     VIAFEGDGLLNEYVGGYEDWARSKSAKDNLTQLLLQTSKISVPTEEKKSRLSPKLSYH
                     EVRELAALPEKIDSLEREQTLINQQLSDATLYKSNPEQIKVLQTRLTKIEEQLTQFFS
                     RWEELEAKQSAAKM"
     gene            complement(6665..7102)
                     /locus_tag="PK522_07570"
     CDS             complement(6665..7102)
                     /locus_tag="PK522_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNC41628.1"
                     /translation="MAITRYEPWGLLTQLQKELEQMRSDVAGDGSSATAEWAPAVDIK
                     EESDRFVLHADLPGVKPEEIDVSMENGVLTIRGEKKTEAKTEKEGYKRIERTYGSFYR
                     RFSLPDTANPDAISAVSKNGVLEITIPKRESVQPKKISVAAVE"
     gene            7538..8272
                     /locus_tag="PK522_07575"
     CDS             7538..8272
                     /locus_tag="PK522_07575"
                     /inference="COORDINATES: protein motif:HMM:NF016291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HNC41629.1"
                     /translation="MSYGDIVGRLIKVLANEGTSKLEHIIKSSQQSQTASGVWDFVLN
                     FFRTKQAGNMTGSGIGSLGVIAGALLNGGAAAVKGAMGGGVLAMLGTMAINALQKRLN
                     IQSADIESGLLSKFLSNEDIASMSSAETEKLVLSAMINAAKADGQLDQQEMDKILGKA
                     GVDGISDDEKKFIQDELSKPFDLQNLVSAVPNQVVGAQVYAASLFAIDINTDAEKDYL
                     KNLAQALELDGETIARLHEMTGAPLV"
     gene            8384..>8877
                     /locus_tag="PK522_07580"
     CDS             8384..>8877
                     /locus_tag="PK522_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HNC41630.1"
                     /translation="MATANLLALLDDIASVLDDIAVLSKLAAKKTAGVLGDDLALNAQ
                     QVMGIQANREIPVVWAVAKGSFWNKAILVPAALLISALLPWLVTPLLMIGGAYLCYEG
                     FEKLAHRFLHDAVEETTHKKALSKAIADPAIDLVAYEREKIRGAIRTDFILSAEIIVI
                     ALGIV"
BASE COUNT         2600 a         2035 c         1751 g         2491 t
ORIGIN      
        1 gcaacataaa acgccctgct ctggtccctg catcgaccgc cgtcaaaata aataaggctt
       61 cgaatagaat cgcaaagtgg taccagaatg ccatcatggc ggtaccgcca actacctcag
      121 acaaaatttg cgccataccc accgccaacg taggtgcacc gccagtgcgc gaaataatgc
      181 tgtgttctcc aacattctgc gcggtttggt taatcatttc cggtgttaca taaaaccccc
      241 attgtgcaat ggtctgcgcg gctgattcgg cagtgctacc aatgatcgca gcggggctat
      301 tcattgcaaa atatattcct ggctcaagcg tcgcggcagc gaccaatgcc ataatggcca
      361 cgaaggactc cattagcatt gccccataac caataaaacg cgcatcggtt tcattctgaa
      421 tcattttagg cgtcgtgccg gatgcaatga gcgaatgaaa tcctgaaatt gcgccacatg
      481 ccacggttac aaacaaaaag ggaaataagt cgccagacca gacgggacca gaaccatcta
      541 caaattgggt caatgctggc attttaagat ctggcgcaat gatgagcact cctatcgcaa
      601 gcccaaccga tcgtaccgat tttcaggaat gtcgagagat aatcacgtgg cgctaataac
      661 agccaaacgg gtaataccga agcaacaaaa ccatagccaa tcagcgccca agtcaattgc
      721 tccccggtta gggtaaaaat cgcagcccat gcagggtctt cctgtacata ttgcccaacg
      781 acaatcgata gcatcagcaa tacaaaaccg atgagcgaaa cttcaccaat cgcacaaggc
      841 cggaaaaaac gcgaataaat tcccataaac atcgcaatag gaatcgttgc tgccacggta
      901 aacgtccccc atggtgattc tgcaagcgcc ttgaccacaa tcagcgacaa aaccgctaac
      961 aaaatcagca tgataaaaaa cgcaccaaac aacgcgatca tccctgggat ttgccctagc
     1021 tctgacttga tcaaatcacc gagtgaacgc gcattgcgtc tcgtcgatac gaacaacacg
     1081 ataaaatcct gcactgcacc cgctagcaca acacctgcca gcaaccacag catcccaggc
     1141 aaatacccca tttgcgcggc tagaatcgga cccacgagcg gacctgcacc cgcaatcgcc
     1201 gcgaagtgat gaccaaataa cacgaatttg ttcgtcggaa catgatccaa tccatcgtta
     1261 tgcttatgcg ccggcgtcat tcgcgttgca tcaagcttca acacgtgatt ggcaataaat
     1321 aagctataga atcgatacgc aatcaaatag acacagactg ctgcaatgac gatccacaac
     1381 gcgccaatcg attcacctcg attgagtgcg atgactgcga gagcagaagc accgaggaga
     1441 gaaaagagta accaacctag tttattcact atcgaattca tatctaatta gtatcaaaaa
     1501 attattatca aattgactac ttgaatggag cgatgctagc gaatttagtc tacaggttgc
     1561 ttaacgtacg gtaccgagca agattccttg ttctttgagc tgctgcatca attgctcccc
     1621 gtaggttacg atcatatcag gttgaggatg attcatctca actgcgatgc gctgtaaaat
     1681 cgtttgcccc gattgattgg tattatgggt aatgcgttgg agcaaatgca cggtaatggc
     1741 gtttgtttca atgaagtgaa tgtcaaattc ggcatcacga aatacgatca gatgagttgg
     1801 attcgcttca tcagcttgtt gaggttgaaa agtcggtgaa attttgtgga caggaaaacg
     1861 ataactcagc acccaggcca aaggtgaaat aacaggtatg ccttcgagca aatcaccagt
     1921 taggttaatt gcgttgttat cgatcttttc atcaaataag gatagcgcta gctctaccca
     1981 ttcgtaatgc gcaagctcta acagaaaagg aggatcatca ggctgaggtt ggcgctcatg
     2041 ttcgagatat ttcagaaact cccttggcat ttccggaaac aagggggttt gcgctaaatg
     2101 gtttgcaaaa taatcctgaa ccatcgcatg ccagcgatct tcagtcataa tgttatgcag
     2161 caccgggtaa gtattggcga tgaacgcttc tacattgttg tagaacaatt cacgatacac
     2221 ggccatacgc cgcgcttcga ttccttcagg gcagggaaat tgcttagggt tacgaatatg
     2281 tgcagcaaaa gcatattgct gccgaataaa gtcaggacgg tcgggcgtgt tgtatgtggt
     2341 cacaagcgca atttagccac ttggcctgca aatgattgat aattttagcc tcttctaata
     2401 attcagaaag tgccggaata ttaaaatcgc gttccaacaa tgtcggtaat acaccaaagt
     2461 gctgatacgc tttttccagt aattgccaga ccggatcaat gaccgaagat ccatgggtat
     2521 cgaccaataa atcttccgct tcctgataat gccctgcaac gtgaatataa cgaatgcgtt
     2581 ctgcgggaag ctgtcgcaaa aacgcttctg catcgtagcg atgattgata ctgttgacgt
     2641 aaatattatt gacatccaac aacaaatcgc aatctgcttc ttctaagact gcattcagga
     2701 aatcgatttc ttccatttgt tttccaggtg cggcgtagta ggaaatattt tccatggcaa
     2761 ttcgttgatc cagcatatct tgaacatgcc gaatccgctc tgccacatag cgcaccgctt
     2821 cttccgtaaa aggaatgggc atcaaatcgt acaaatgccc atcatcacta caatagctga
     2881 ggtgctcgct gtaataacgt atgcgatgct ctttcaaaaa ttgcttcaaa cgctgtaaaa
     2941 atgtttcgtc gagtggtgct ggactcccga tggaaagcga taagccatgg caaataacag
     3001 gatgcttctc ggtaagttct cgaaattgct taccgtgtcg tccagcaaca ttaatccaat
     3061 tttcaggcgc gacttcccaa aaattaactg attcggtgtt ttggtctgca agcgtgctca
     3121 acatcacacg ccgtagacca agcccaacac catgaacagg atatttaaaa ctgttcatgg
     3181 taaaacctta ttatttcttg ttggagctac cacacttacc ttcaccgcat tttccttctt
     3241 gctcggcttt cttaccgcca cactgtcctt ccatattttt cttcatctca cctttagcac
     3301 cacacttgcc ttccccacat ttaccttcat gatcagcctt tttttgatct gtagatggct
     3361 gactatcttg ttcacctttc ttggcatcat cagcagaagc gagctgcata tagccactcg
     3421 ataaggtttt cattgcaaaa ggattactct cagccgcaaa attgctggct gataatgtgg
     3481 tcaatagcgc tgtacctaaa gcaattgaga caggtttgat gattttcttt gacattaaac
     3541 taattctcca taaaaagtta atctacgatg attacaaatt ggacaagcag ttaatatagc
     3601 agatttcttt gaagttataa ccctaacggt aaagatagga aaatcggata agcaggagaa
     3661 aaaatcgctg gtagcgttcc gaaaaataag aaagcgaata tgaaacattc catattcgcc
     3721 ttttcgaaac aactttattt ttaaagtagc gcactaatga atgccctact ttattgatct
     3781 aatacactta ctttttcatt gtcaagccaa tattttgact tgagtagtaa aaagcgagat
     3841 tgccgatatc atctggagtc aacgctgctg ccattcctgc cataactgga tctttgcgat
     3901 cgcctgtttt atagtctttt agtgcttttt caagataagt tcttggttga ccgccaattt
     3961 taggaaaatt aggcgctggg ctattgccat ctgctccatg gcaagaagca catacttcag
     4021 cggcttttct tttacctgct tcaatatctg ctgcaatcgc atttcccgaa ataacaaaaa
     4081 ttgctccggt tactgcagca ataaaataat tcttcataat cttattatcc ttgcgtaaat
     4141 gggaaattag ttacctgcat agtatgcagc taattcttct atatccttat cagaaagggt
     4201 agctgcaatc gctttcattg taggatgatt acgctcaccc gctttatagt ctctcaatgc
     4261 ttttgctaag tacacagcat gctgcccacc caattttggt acatgataag caactggata
     4321 agcagtacgg taaccctcta ttccatggca tccttcgcac atagaagttt tacttttcac
     4381 tccttcagca tcactggccg catgaacccc accagaaatt gccaaagcca agccgactgc
     4441 aaacgctaat ttaacggcca actttttcac tttatgcatc ctcctctcaa aaatcagaac
     4501 tttaaacgat gggggctgtt tagtcaatga agataatcgc tatctccctg ttgtttatag
     4561 ggaaaaaacg ctcaatccct acatttttgc tgcggattgt ttagcctcta attcctccca
     4621 gcgcgaaaag aattgagtga gctgctcttc aatcttggtc aaacgcgttt gcaatacctt
     4681 aatttgttca gggttacttt tatagagtgt tgcatcactg agttgctgat taatcaaagt
     4741 ttgttcacgc tctaatgaat caatcttttc tggtagcgcc gctagctccc gcacttcgtg
     4801 ataactcaat ttgggcgata atcttgattt cttttcttca gtcgggacag aaattttact
     4861 tgtttgaaga agtaactggg tcaagttatc tttggcactt ttacttcgtg cccaatcttc
     4921 ataaccacca acatactcgt ttagcaatcc atctccctca aacgcaataa cttgtgtaac
     4981 cacattatct agaaaagtac gatcatggct gactaaaaac agggttccgg tgtaactctg
     5041 caataaaact tccaataatt ccagcgtctc gatatcaaga tcattcgtag gctcatctaa
     5101 tacgagcaaa ttagcgggtt gggtgaacag acgcgccaat aacaagcgat tacgctcccc
     5161 accggataat gatttaaccg gtgaacgcgc gcgctgcggt gcaaaaagaa agtcttcaag
     5221 ataactgata acatgcttac gctcaccatt aatctcgata aaatcagagc cttgattaat
     5281 cgcctcgata actgtggatt ctggatttaa ttgacctcgc atttgatcaa aataagcaat
     5341 ggataatttc gttcctcgct ttatttcccc taaatcaggt tccaattcgc ctagaataag
     5401 ttttagtaag gtcgatttac ccgaaccatt agggcctaat aaaccgattc gatcgccacg
     5461 aagaatgcga caagaaaaat cctttatcac ggttttatca ccaaaacatt tggtaacctg
     5521 ctgaagttct gctactaaat gtcctgatcg ctcacctact tcaacattga aattgacaga
     5581 cccaacacgg gtgcgacgtg ccgcgcgttc caaacgcaat gcttccaaac gtcttactcg
     5641 cccttcatta cgcgtacaac gcgcttgtac accttttcgt atccatacct cttcttgcgc
     5701 aagcacctta tcgaatttgt gctgttgtgt cgcttcactt tgcaacattt ccattttctt
     5761 ttgcaggtaa tgcgcaaaat tacctgtaaa actcccaagt aatccgcgat cgagctcaat
     5821 aattcgagta gaaacgttat ctaaaaagcg ccgatcatga gtgataaaaa tcacactgcc
     5881 tgcaaaagtc tggagtaatt cttctagcca ttcaatcgaa gtaaaatcaa gatggttagt
     5941 aggttcatcc aataacaata catcaggcaa cacgactaaa gcacgcgcta acgcgactct
     6001 tttacgggtt ccacctgata aatttccaat cagatcttgt gctgataatc ctaattggtg
     6061 aatgacggtt tcaactctcg tttggatccg ccatccatcg tttaactcca aaaaattctg
     6121 caatgatgcg agtcgagaaa agttggtttc attgcttccc ttgccttcac tcagcgcttg
     6181 ggaaatttca tggtattcat tgagaacttg gctcagatga cccaaccctc gagaaacttc
     6241 atgatagaca gtacctgctg tcccaaattc aggctcctgc gaaacataaa caagcttcag
     6301 ttctggttta tgccaaagct ttccatcgtc aagcttaatt tctttcgcta gtacttttaa
     6361 caaacttgat ttgccactgc cattgcgtcc aatcaagccg attcgttctc ctgggtctaa
     6421 ttggagatca acatgattca gtaaggcgtg atggccaaag gctaagcagc tatcctcaag
     6481 ggtaataagt ggcataagta aaagtacaaa taatcaacat caaataagat ggtgcgattt
     6541 tttcatgaat tataggatgc agcaaactag ttcgctgatt gcttattatc ttgaatcgtt
     6601 taattcaaaa gaaaaaggga atcattagat tcccttttta atttactaaa agtaataaac
     6661 ttaattattc tactgctgcc acactgattt tcttaggctg taccgattca cgttttggaa
     6721 tggtaatttc cagtacgcca tttttagaaa cagctgaaat tgcatctgga tttgctgtat
     6781 caggcaggct aaaacgccta taaaatgaac cgtaagtccg ttcaattcgt ttatagcctt
     6841 ctttctcagt ttttgcttca gtttttttct cgcctctgat ggtcaataca ccattttcca
     6901 tactgacatc gatttcttct ggttttaccc ccggcagatc agcatgtaaa acaaaccgat
     6961 ctgactcttc tttgatatcg acagcaggcg cccattctgc ggtagcagaa gagccatcgc
     7021 cagccacatc actccgcatt tgctccaact ctttttgaag ctgtgttaat aatccccatg
     7081 gttcataacg tgtaatagcc ataattcatt atctccaatt ataaatatgc aaattacctg
     7141 caattaaaat ttcctatata tcctgatgct atagtgccat tgaacatatc tgagcatctc
     7201 caaatactca tcatcaacga tcgtatgcaa taacgagtac tgcttgtaat atagggatgc
     7261 gcctaccatt ttcaagcaga taaaatgatt tttttaatcc ctcattatca taggattgct
     7321 atgcaccgca taactcaagt gctggcaatc tagtcaactc atttaaaaga aattcttata
     7381 gtgaagcttt atcttcattc actgagttgc gctataactt cccaaaatgc tggtatgatt
     7441 accctggcac gtatcacaaa tcataggaaa ccttttcatc tttatctcat gcaattgcta
     7501 tagataaagc catctcaatt cacagaaagg aaaaaaaatg agttatggag atattgttgg
     7561 tcgcttaata aaagtattag cgaatgaagg aacctcaaag ctagaacaca tcatcaaatc
     7621 ttctcaacag tcccaaaccg caagtggcgt atgggatttt gtgttgaatt tttttcgaac
     7681 aaaacaagct ggcaacatga ctggcagcgg aattggtagc ttaggggtta tcgctggcgc
     7741 cctattaaat ggaggtgctg ctgctgtaaa aggagccatg ggtggaggcg tcttagctat
     7801 gctgggtacc atggcaatca atgcgctcca gaaaagatta aacatccaat ccgcagatat
     7861 cgaatcggga ttattgtcaa aattcttatc gaatgaagat attgcatcga tgagctctgc
     7921 tgaaacagaa aaactagtac tcagcgcaat gatcaatgct gctaaagctg atgggcaact
     7981 tgatcaacaa gaaatggata aaattcttgg gaaagctggg gttgacggaa tttctgatga
     8041 tgaaaagaaa ttcattcaag acgaattgag taaacctttc gatttacaaa atctagtatc
     8101 tgctgttcct aatcaagttg tcggagcgca agtttatgcg gcatctttgt ttgcaatcga
     8161 tattaacact gatgcagaaa aagattattt aaaaaatcta gctcaggccc ttgagttgga
     8221 tggtgaaacg attgcacgtc tgcatgaaat gaccggtgcg ccactggtat aaataattct
     8281 tgtataacca ggaatgtttg acgcctcgct aacgatagcg aggtgtttat ctgatatcgt
     8341 taccaaaatt taagcttttt tgaaatccta ttgattaacc tcgatggcaa ctgcaaacct
     8401 tctcgcactc ctcgatgata ttgcgagtgt tttagacgat attgctgtgc taagtaagct
     8461 cgccgctaaa aaaacagcgg gggtactggg agatgacctt gcgcttaatg cgcagcaagt
     8521 catgggcatt caagctaacc gtgaaatacc cgtggtgtgg gcagttgcaa aaggctcatt
     8581 ctggaataag gcaatcttag ttcctgcagc gttactgatt agcgctttac tcccttggtt
     8641 agttactcca cttctgatga ttggcggtgc ttatctttgc tacgaaggtt ttgagaagct
     8701 tgcacaccga tttctgcatg atgcagtaga ggaaacaacg cataaaaaag cattatccaa
     8761 agctatcgct gaccctgcta ttgatttagt tgcctatgag cgcgaaaaga tcaggggagc
     8821 tatccgtacc gattttattc tgtcagctga aataatagtg attgcgttag gtattgt
//
LOCUS       DAOQLJ010000094         8812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3130_length_8812_cov_6.440219, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000094 DAOQLJ010000000
VERSION     DAOQLJ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8812
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3130_length_8812_cov_6.440219"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PK522_07585"
     CDS             complement(<1..675)
                     /locus_tag="PK522_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41631.1"
                     /translation="MSVDEYQYRVIGIEQPTVASPQVKEVKVIKKRSLVPGGTRSANV
                     GDFDIKANEIVHLKLNNGFSLWLRADDLLDEFGKPNISRGSQHTWEIRVDPAKFQANR
                     SARGLAGLAIKVLDFFDVDLQSYTASKLCSLWEDKLLEKKAANLYRCSLEDTYALSEK
                     GELKYEQPLLLFIHGTASNSQKSFGGLWEASSKVGKAARERLKKKYGEAVYAFEHRTL
                     TESPIQN"
     gene            complement(754..1590)
                     /locus_tag="PK522_07590"
     CDS             complement(754..1590)
                     /locus_tag="PK522_07590"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNC41632.1"
                     /translation="MAKKALCIGINNYPGTHMDLQGCVNDAQDWSNVLQSRGFVISAL
                     LDQQATKAAMMSAMSNLINPAVAGDSLVITFSGHGTYQPDKDGDEVDGLDEALCPYDI
                     QTKGEALTDDEIHTLFKVRKPGVKLVLISDSCHSGTVTRAAAAEPEADAPRPRFMPMG
                     NWMSDKDLPHSRSGGIAPFINIGASTSPFLAAYTRSEDDLLLSGCQEGPNNFSYDAKI
                     SGRYNGAFTYYALKTLKTLSVNATYKDWHDAIRRYLPSASYPQTPQLVGTLTARQMKV
                     FS"
     gene            complement(1905..2225)
                     /locus_tag="PK522_07595"
     CDS             complement(1905..2225)
                     /locus_tag="PK522_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41633.1"
                     /translation="MKRILLLGMLLIPLITMAEDLSLRQRANYAFEQMKAAERDLTTI
                     TQTIEVKKGRLEYFKQKAIEAEQELANAYKESQEINKRVTSTRNRWEQLSNQLLQGDS
                     ESRD"
     gene            2454..2723
                     /gene="rpsO"
                     /locus_tag="PK522_07600"
     CDS             2454..2723
                     /gene="rpsO"
                     /locus_tag="PK522_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNC41634.1"
                     /translation="MAVTTEQKAQVISDYQRIKGDTGSPEVQIALLTTRINDLIGHFK
                     AHVKDHHSRRGLLKMVNHRRKLLDYLKRKNSESYLKIIERLSLRK"
     gene            2858..4981
                     /gene="pnp"
                     /locus_tag="PK522_07605"
     CDS             2858..4981
                     /gene="pnp"
                     /locus_tag="PK522_07605"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNC41635.1"
                     /translation="MKLTKKSFTYGRHEVTLETGEIARQAHGAVIVTMGETVVLVTVV
                     GDKNAKPGQDFFPLTVDYQEKFYSAGRIPGSFFKREGKPSEKETLTSRLIDRPIRPLF
                     PAGFYNEVQILAMVLSSDSEIDADIPAMIGTSAALVISGIPFDGPIGAARVGYFNNEY
                     ILNPTTTELKNTQMNLVVAGTQQAVLMVESEATELSEDVMLGAVVYGHEQMQIVINAI
                     NEFADEAGVVPWEWQPVERDPALIEKVTQAAEADLRNAFKIKQKQARTEAIKAVWQRV
                     YADLGIGTEEGPDEHIVNEIGFDLEAKIVRNQILDGEPRIDGRGTRTVRPITIRHGLL
                     PRTHGSALFTRGETQALVVATLGTARDEQKIDALQGDYSERFMLHYNMPPFATGEIGR
                     VGTPKRREIGHGRLAKRALVSVLPSTEEFGYSLRVVSEITESNGSSSMASVCGGCLAL
                     MDAGVPLKSHVAGIAMGLIKEGNRFAVLTDILGDEDHLGDMDFKVAGTENGITALQMD
                     IKIQGITKEIMQVALLQAKEGRLHILNIMKDALPANRESISTHAPRIIKFKINPEKIR
                     DVIGKGGAVIRALTEETNTTIDITDDGTVMIACVSSEGGEQAKKRIEDITAEVEVGKI
                     YVGTVLKLLDFGAIVSIFPGKDGLLHISQIANERVEDVAAHLKEGQSVRVKVLEADDK
                     GRLRLSMKAAIADDIDNASSAPQPN"
     gene            complement(4997..5884)
                     /locus_tag="PK522_07610"
     CDS             complement(4997..5884)
                     /locus_tag="PK522_07610"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNC41636.1"
                     /translation="MLDLDKKQFSKHFRTILEKIEQIPPFPESARLLSDLRQNPDANI
                     DQLSEIIESDPGLTAFILKYARIPILGYGHRIHSVDQAIRLVLGFNTASSLMMSIASS
                     SCLRMQKHGQLGYIKIWSKTLECAALCRELSLMMSNRSQIDSDLAHLCGLFQNFGYLL
                     FGHFFPKEYSYLNDLVARYPEQSFRTIQLQFFGITHDTIGKYLMNAWNLPEEVIVAIA
                     EQNYPDYSGKHAIYVKLIAVANRLLQSELQNIDGCAYVETTSLIDDLGINENEALKAK
                     ENILGCQNDFAILSQSLAA"
     gene            6154..7860
                     /locus_tag="PK522_07615"
     CDS             6154..7860
                     /locus_tag="PK522_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNC41637.1"
                     /translation="MPTYIFIALLAPFIMLSSVTYGASKLPATVKVNPEALTSEPMCD
                     PVIAPSWREAQEIEGVKIEASPVCSPDNPAWIAAAVKGTNNISMDTLMKTGLSPDAII
                     KTDDIDGDGDPDRITIRLEIMELNGKSPDFPGIVPTFDIAPGIQPGAWVFSPKMSGMS
                     TENFESIRANPLLRLPSPVIRVEVGDIVQIVLENTHYFPHSIHLHGVDHPFAHHEHGG
                     QDGVPQTSEIELMPGDRRVYQFQARQPGTMFYHCHVQTHTHLAMGLAGMIIIEENKPN
                     NWLQTLNIGAGHVRHPSVAVREHYDQEYDLHYHAMDKELGQIIQRHNDPRLIARDMNQ
                     RYDITDSTEDYYTLNGLSFPYTLRESLIVVEPDQKIKLRLLNSGGELIAIHTHGHHAT
                     ITHYDGVEHNPVAQIMRDVFDMAPAQRLDLKLETVNDGKHSYGEGDWLIHDHREKGIT
                     TNGMAEGGSMSSIVYKSYLGESNMPKVSHFGIDLNKYFTKEYYERRLPVWQDLDEWSS
                     LGSPGEGSGLEDKTQTSLIYGAIGLLFGLMFYWIFSKRKSITQVMLGTISRINASKGS
                     NQ"
     gene            7860..>8812
                     /locus_tag="PK522_07620"
     CDS             7860..>8812
                     /locus_tag="PK522_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNC41638.1"
                     /translation="MNRNILSLLIAGALTASTYAVAQPKDTINDEHAHHDHSSHSIEQ
                     HKEQSTDQNHDHSHHSEHQHSSDHAISHEHDHATDHNQHTTHSPEHSVSGADDHHHHH
                     MDHDHIMDHEGTMIMGQNFDKLPSSCQSISEEVEITVRAGRKYSAKFPGTAFAFDQQQ
                     WKVKPCSKITWHFVNEDNIRHQFMMHGLPKYLYKLGMFHLEVTGPKTVSGTMIVPGKD
                     ETFLVHCDISHHMEKGMKAQLVVGKGGTDIPSIPGLTPYNLTDTYEAENLPKVGDKVE
                     SSKMVRQITQFTSDNLQTYGVMLIGILLGLLCIPFIKKILKL"
BASE COUNT         2642 a         1830 c         1814 g         2526 t
ORIGIN      
        1 attctggata gggctttctg ttaatgtacg gtgttcgaaa gcataaactg cttcaccata
       61 ttttttcttc aatctctccc gcgctgcttt acctactttt gaagacgctt cccacaatcc
      121 accaaagctt ttctgactat tcgaagcggt tccgtgaatg aagagcagta aaggctgttc
      181 gtactttaat tcccccttct cgcttaatgc gtaagtatct tctaaggaac atcgatacag
      241 attagctgct ttcttctcaa gaagtttatc ttcccataat gaacaaagct tacttgcagt
      301 ataggattga agatcgacat cgaagaaatc gagtacctta atggctaacc ctgccaaacc
      361 tcgtgcgctt cgattagctt ggaatttcgc tgggtcgact ctgatttccc aagtatgctg
      421 gcttccacgg ctaatattcg gtttcccgaa ctcatccaat aaatcatctg cacgcagcca
      481 caaggaaaaa ccattgttta acttaagatg aacgatttca ttggctttaa tatcaaagtc
      541 acccacattc gcagatcgag taccgccggg caccaagctt cgttttttaa tgaccttgac
      601 ttccttaact tgcggggaag cgactgtcgg ttgttctatt cctatgacac gatattggta
      661 ttcatcaacg ctcattactc tacctccgat catctaagct cagtagattt agaatctaca
      721 attcttatct gcacttaata attcgctgaa ttactaggaa aaaactttca tttggcgcgc
      781 agttaatgtt cctaccagtt gaggagtttg aggataacta gcagaaggta aataccgcct
      841 aatcgcatca tgccagtctt tatacgttgc attgacactt aatgttttaa gggtttttag
      901 cgcataataa gtaaatgcac cattgtagcg tccgctaatt tttgcatcat agctaaaatt
      961 gttggggcct tcttggcaac ccgaaagcaa taaatcatcc tcacttcttg tataagccgc
     1021 caagaaaggc gaagtactcg caccgatatt gatgaatggc gcaataccgc cgctccgact
     1081 gtgcggcaaa tccttatcag acatccagtt gcccatcggc ataaaacgag ggcgtggtgc
     1141 atcggcttca ggttccgctg ctgcggcacg tgtaactgtt cccgagtgac agctatctga
     1201 aataaggact agcttgacac ccggtttgcg cactttaaag agcgtgtgaa tttcatcatc
     1261 cgttaaggct tcccctttag tttgaatatc ataagggcaa agcgcttcat ccaacccatc
     1321 gacttcatct ccatctttat caggttgata ggtgccatgc ccagaaaacg tgatgactaa
     1381 gctatcccct gccaccgcag gattgatgag attactcatt gcactcatca tggctgcttt
     1441 ggttgcttgc tgatccagta aagcgctaat cacaaatcca cgcgactgca atacattgct
     1501 ccagtcttgt gcatcattga cacaaccctg taaatccatg tgtgttcctg gataattatt
     1561 gattccaata caaagtgctt ttttagccat atcgccctcc tgtgtatttt tatggtgttt
     1621 cacatacagc ctaaccattg ctgagatggt aacgtagaaa tagcgcctat tcgattcaac
     1681 cgacctctca gcaatcatgc cagaaaaata aatcgcggtg tatgattttg atcaactgaa
     1741 gagatcagat gaatgagcaa tagaagctta gctcggtatg gaaaaaagag gtgctactga
     1801 agcagggcaa gaacggaaat ctgcttatat ttccccaaat aggctattgt ggaaattgtc
     1861 ttgatatcga tcgatgcgtt acaacaagtt aggccattat tactttaatc gcggctttca
     1921 ctatcacctt gtaacaattg attggaaagt tgttcccaac gattccgggt cgatgttact
     1981 cgtttattga tttcttgcga ttctttgtaa gcgtttgcta attcttgttc cgcttcgatt
     2041 gctttttgtt tgaaatattc caaacggcct ttcttaactt caattgtttg agtaatggtt
     2101 gttaaatctc gttctgctgc tttcatttgc tcaaacgcat aatttgcgcg ttgccgcaag
     2161 ctcaaatctt cagccatcgt tattaagggt atcaatagca tcccgagtag caaaattctt
     2221 ttcatacgtt ttccttaatt tctattttac aaacttaacc caatagggcg ctcagtttat
     2281 ctgttatcct cattacaaac aatttagtaa aaattgatgt agaacaaatc gaagtcaaag
     2341 ttgatattca ccctttttgg acaaaatatg gattaaagag tagaatttcg ccttcattta
     2401 cagatgatag gtaattgaat aagctacttt ttaactgaac tggagaaaat tttatggcag
     2461 tcacaacaga gcaaaaagcc caggtgataa gtgactatca aaggatcaaa ggtgataccg
     2521 gctcccctga agtgcaaatt gcacttttaa caacgcgtat caatgattta attggtcact
     2581 tcaaagcgca tgtcaaggat catcattccc gccgcggatt gcttaaaatg gtcaatcatc
     2641 gacgtaagct attggattat ttgaaaagaa aaaatagtga aagttatctc aaaattattg
     2701 agcgtctaag tctcagaaaa taattggtaa ctagaattta ttgttagtat gaaacgggtg
     2761 ggaataatta cgtaagtgta agatttttga gcgtttgatt cttgttttgt tacataacta
     2821 aagaaaaacg agtatacaaa tatagaagga tcgttaattg aaattaacca aaaagagctt
     2881 tacctacggt cgtcatgagg tcacattgga gaccggtgaa attgcgagac aagcccatgg
     2941 cgctgtgatc gttacaatgg gggaaacggt tgtattggta accgtggttg gtgataaaaa
     3001 tgcaaaacct ggtcaagatt ttttcccatt aactgttgat tatcaagaaa aattttattc
     3061 tgcaggtcga attcctggta gcttttttaa gcgtgaagga aaaccttccg agaaagaaac
     3121 actgacctct cgattgatcg atcgtccgat ccggccgcta ttccctgctg gtttttataa
     3181 tgaagtgcaa attcttgcca tggtattatc ttcagatagc gagatcgatg cagatattcc
     3241 cgcaatgata ggtacttcag cagcattggt tatttctgga atccctttcg atggccctat
     3301 tggcgcagct cgtgtgggtt atttcaataa tgaatatatt ctcaacccaa caacaacaga
     3361 gctcaaaaat acgcagatga acttagtcgt tgctggaaca caacaagctg tattaatggt
     3421 tgaatcagaa gccacagagt tgtccgaaga tgtcatgcta ggtgctgtcg tctatggcca
     3481 cgagcaaatg cagatcgtaa tcaacgcaat caatgaattt gctgatgaag ccggcgtagt
     3541 tccttgggaa tggcaacccg ttgaacgaga tcccgctcta atcgagaaag ttacacaagc
     3601 ggcagaagct gatcttcgta atgcatttaa aatcaagcaa aaacaagcta gaactgaggc
     3661 aatcaaagct gtctggcaac gtgtttatgc ggatctaggg attggcactg aagaaggccc
     3721 tgacgaacat atcgttaatg aaattggctt tgatttagaa gcaaaaattg ttcgtaacca
     3781 aattttagat ggagaacctc gcattgatgg acggggaact cgcaccgtta gaccaataac
     3841 cattcgccat ggcttattac cgcgtactca tggatctgca ttatttaccc gcggcgaaac
     3901 acaagccttg gttgtggcta cattaggaac ggctcgcgat gagcaaaaaa ttgatgcact
     3961 tcaaggcgat tactctgaac gttttatgct gcattacaat atgccgccct ttgcaacagg
     4021 tgaaattggg cgtgtcggca ctcctaaacg ccgagaaatt ggacatggtc gccttgctaa
     4081 gcgtgcactc gtttcagtac tcccttcaac tgaggaattc ggatactcat tacgcgtcgt
     4141 ttcagaaata acggaatcca atggatctag ctctatggct tctgtctgtg gcgggtgcct
     4201 tgcactaatg gatgcaggcg ttccactcaa atctcatgtt gctggtattg caatgggatt
     4261 gatcaaagaa gggaaccgct tcgcagtttt aactgacatt ctcggcgatg aagatcatct
     4321 cggcgatatg gattttaaag ttgcgggcac tgagaatggc atcaccgcac tacaaatgga
     4381 tattaagatt cagggtatta ccaaagaaat tatgcaggtt gctttgttac aagccaaaga
     4441 aggtcgccta catattctca atataatgaa agatgcatta cctgcaaacc gagaatcaat
     4501 ttcaacgcat gcacctcgta tcattaagtt taaaataaat cccgaaaaaa tccgtgatgt
     4561 gatcggtaaa ggtggtgctg taattcgcgc attaactgag gaaaccaata cgactattga
     4621 tatcaccgac gatggaacgg tgatgattgc ttgtgtcagc agcgaaggtg gtgagcaagc
     4681 caaaaaacgg attgaagata ttactgctga agttgaagtt ggaaaaatct atgtgggaac
     4741 tgttttaaaa ttactcgact ttggcgcaat tgtaagtatt tttcctggga aagatggact
     4801 attgcacatt tcacaaattg caaatgagcg cgttgaagat gttgctgctc accttaaaga
     4861 aggacaatca gtacgcgtta aagtgctaga agctgatgat aaaggacgcc tgcgtctgag
     4921 tatgaaagca gcaatagcag atgacattga taatgcctct tctgctccac aaccgaacta
     4981 ggaaaattaa taactgttag gctgctaaac tctgactcag aatcgcaaaa tcattttggc
     5041 agcctaatat attttctttg gcttttaagg cttcgttttc attaataccc aagtcgtcaa
     5101 ttaatgaagt cgtttctaca taagcacagc catcaatatt ttgtaactca ctctgtagta
     5161 aacgatttgc cactgcaatc aattttacat agatcgcatg ttttcctgag taatccggat
     5221 aattttgctc agcaatcgca acaatcactt cctctggtaa attccaggcg ttcatcaagt
     5281 attttccaat cgtatcatgt gtgataccga agaattgaag ttgaatggta cgaaaacttt
     5341 gctctggata acgagcaacg agatcattta aatagctata ttcttttgga aagaaatgac
     5401 caaatagcag atagccaaaa ttctggaata atccacacaa atgtgccaaa tcactatcaa
     5461 tctgtgatcg attactcatc attaaagaaa gctctcggca caaagcagca cattctaggg
     5521 tttttgacca aattttgatg tagcctagct gcccatgctt ttgcattctt aagcaacttg
     5581 aagatgcaat actcatcata aggctacttg cagtattaaa acctagaaca agcctgatag
     5641 cttgatcaac actatgaatt cggtggccat aaccaagaat gggtatccgt gcatatttta
     5701 gaataaaagc agtgagccca ggatcacttt cgataatttc actcaattga tcgatattgg
     5761 catcaggatt ttgtcgaaga tctgaaagta accgagctga ttcaggaaag ggtggtattt
     5821 gttctatttt ctctagaatg gtgcggaaat gtttactgaa ttgtttttta tcaagatcta
     5881 acatcaatcg atcgccttta tcgtaaaaat gtgtacatga agttgatttt tgattttctc
     5941 caaactaaat tctaagttgc tgctactcac ggtatgttta gctgaagaaa tcacttcaat
     6001 tgaacgacaa gttgtctacg ttcttaatta aatttaactc ggttaaatcc ctatgctagc
     6061 tagcagttgg taaaaccaac aaaaccacac tagggtatct actgtataat gtagggttat
     6121 atttagttaa tcattgaatt tagagtatgt aatatgccaa cctatatttt tattgcattg
     6181 ctagcccctt tcatcatgtt aagttcggtt acttacggcg cttcaaaatt acctgcaacc
     6241 gtaaaagtaa acccagaagc tttaacatca gaaccaatgt gtgacccagt gattgcgccc
     6301 tcatggcgtg aagctcaaga aattgaaggt gtcaagatcg aagcttcacc cgtttgcagc
     6361 ccagataatc ctgcatggat tgcagcagca gttaaaggta ccaataacat atccatggat
     6421 actttgatga aaacaggctt atctccagat gctatcatca aaactgatga tattgatggc
     6481 gatggtgatc ctgaccggat tacgattaga cttgagatca tggagcttaa cggtaaatca
     6541 cccgatttcc ccggaatcgt accaaccttc gatattgctc ctggtataca accgggtgca
     6601 tgggtgtttt cacctaagat gagcggcatg tcaaccgaga attttgaaag catacgcgct
     6661 aatcccttac ttcgcttacc ctccccggtt attcgggttg aagtgggtga tattgttcag
     6721 atcgtgctag aaaatacgca ctattttcct cacagcattc accttcatgg tgttgatcac
     6781 ccgttcgcac atcatgaaca cgggggccaa gatggcgtgc cacaaacgag tgaaatcgaa
     6841 ttgatgcctg gcgatcgccg tgtttaccaa ttccaggctc gccaacccgg aacgatgttc
     6901 tatcattgcc atgtgcaaac gcatacccat ttggcaatgg gcctggcagg aatgattatt
     6961 attgaggaaa acaagcctaa taactggtta cagacactca atatcggtgc tggccacgtt
     7021 cgccatccat ccgtagcagt tcgtgaacat tacgatcaag aatatgattt acattatcat
     7081 gcgatggata aagaattagg tcaaatcatt cagcggcaca acgatcctag attaattgcg
     7141 cgcgatatga atcagcgcta tgacattact gattcaactg aggattacta cacgctgaat
     7201 ggcctctcct ttccgtacac attgcgggag tccctaatcg tggttgaacc cgatcaaaaa
     7261 attaaactcc gcctcctaaa tagtggtggg gaattaattg ccattcacac gcatggccat
     7321 cacgcaacga ttactcatta tgatggtgta gagcacaatc ccgttgcgca gattatgcgt
     7381 gatgtatttg atatggctcc ggctcaacga ctagatttaa aattggaaac agtcaacgat
     7441 ggtaagcaca gctatggtga aggagattgg ttaatccacg atcatcgcga gaaaggaatt
     7501 acgaccaatg gcatggctga aggcggtagt atgagctcga ttgtctataa atcctatttg
     7561 ggcgaaagca acatgccaaa agttagccac tttggaattg acctgaacaa atatttcaca
     7621 aaagaatatt acgaaaggag attacccgta tggcaagatc tcgatgaatg gtctagttta
     7681 ggatctcctg gggaaggatc tggattagaa gacaaaaccc agacttcttt gatttatgga
     7741 gccatcggtc tgttatttgg actcatgttt tactggattt tttctaaacg taaatctatc
     7801 acgcaagtta tgctcggaac aatttcacgg ataaacgcaa gtaaagggag taatcaataa
     7861 tgaatagaaa tattttatcc ttattgattg ctggagctct gactgctagc acctatgcag
     7921 tagcacagcc aaaagataca atcaatgatg agcatgctca ccacgatcat agtagccaca
     7981 gtatagagca acataaggaa caatcaaccg atcagaatca tgatcacagc catcatagtg
     8041 agcatcagca ttcgagtgat catgcaataa gtcacgaaca tgatcatgct acggaccata
     8101 accaacatac cactcattcg ccagaacaca gcgtcagtgg tgctgatgat caccatcacc
     8161 atcatatgga tcatgatcat attatggacc atgaaggcac gatgatcatg ggccagaatt
     8221 ttgataaact gccgagtagc tgtcaaagta tttcagagga agtcgaaatt actgtacggg
     8281 ctggtagaaa gtactcagct aagtttcctg gcacagcatt tgcctttgat caacaacaat
     8341 ggaaggtaaa accatgttct aaaattacat ggcattttgt taacgaagat aatattcgcc
     8401 accaattcat gatgcatggc ctaccaaaat atctctataa gcttggtatg ttccatttgg
     8461 aagtgactgg ccctaaaact gtatcaggaa ccatgatcgt acctggaaaa gatgaaactt
     8521 tcctggttca ctgtgacatt tcgcatcaca tggaaaaagg aatgaaagcc caacttgtgg
     8581 tgggtaaagg tggtactgat attccaagta tacccggctt aacaccttat aatttgacgg
     8641 atacctacga agcagaaaat ctacctaagg ttggggataa agtagaatct agtaagatgg
     8701 ttcgccaaat aactcagttc acgagcgata atctgcaaac ctatggcgtg atgcttattg
     8761 gtatcttgct aggattactc tgtatccctt ttatcaaaaa gatactaaag ct
//
LOCUS       DAOQLJ010000095         8786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3154_length_8786_cov_5.851220, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000095 DAOQLJ010000000
VERSION     DAOQLJ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8786
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3154_length_8786_cov_5.851220"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PK522_07625"
     CDS             <1..687
                     /locus_tag="PK522_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41639.1"
                     /translation="PKDNQNSQLVHNSVELASNQPEIHELGSTEGNIKQSEQTEEKSR
                     PRRRRGGRQRNRSDRPRSTATSETLETTSHAVDAIPSIAIASTEPFEIVARSDFEAVN
                     STDHQSQPMNSDAWAEHRLQEVPSEKIERTEDQASSIKAANSVTQDELSVDSASSFKP
                     VTVTAPVVIDLPDLSKSGLVMIETTQENVSEILDDEMLLTRKPRRKTQAAKIDAPETA
                     EPLIQIETKD"
     gene            complement(746..1798)
                     /gene="holB"
                     /locus_tag="PK522_07630"
     CDS             complement(746..1798)
                     /gene="holB"
                     /locus_tag="PK522_07630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNC41640.1"
                     /translation="MNQLYPWQQSVWPQIRNHVITKAQAFIFSGRKGIGQLEFSEYLA
                     QSLLCEQPTQAQFACGSCRSCQWFEQGTHPNFYRLEPEALLVESDLDATQALSDSKQS
                     SEDTNSKKKLSQQITIDQIRHLDQFLYLSGHQSGRKILLIHPADCMNVSAANAMLKRI
                     EEPPPQVLFFLVTDRLQALIPTIVSRCMRITLPHPDRLSVKNWLHQEGIEENSIDLSW
                     FGTSPQLVKRYLEAGYSAYYVELIEKISSPMQLDPITLAEKLHKQELSMIVSWLQKWC
                     YDLVSFQMLGRVYYHHRYEAAIKKIVATTEPKQLIDFWKRLAQFQQWSRHSLNARLFL
                     EELLFAYIKTCSRISN"
     gene            complement(1795..2427)
                     /gene="tmk"
                     /locus_tag="PK522_07635"
     CDS             complement(1795..2427)
                     /gene="tmk"
                     /locus_tag="PK522_07635"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNC41641.1"
                     /translation="MQKGKFITLEGIDGAGKSTHLKWIANYLTNKGIRVLTTREPGGT
                     ELGESIRGLLLDHRQIMHAETEALLMFAARREHLDKLILPALDQGNWVISDRFTDASY
                     AYQGGGRDVSIDKLQILENWVQGQFQPDLTFYFDLSIEVARLRMQSIKIADRFEKEQN
                     DFFHRVRAAYINRAKQFPERMQVLDASRSIDDITHQLEKVLQPLLIEQKP"
     gene            2509..3096
                     /gene="ampD"
                     /locus_tag="PK522_07640"
     CDS             2509..3096
                     /gene="ampD"
                     /locus_tag="PK522_07640"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HNC41642.1"
                     /translation="MKINTAGYLQNAIQIPSPNFDERPLGTTIRLLVIHNISLPPNVF
                     EGNAIIELFTNQLDCNTHPYYEGLRDLKVSSHFLIRRQGEIIQFVSCIKRAWHAGQSC
                     WQGEERCNDFSVGIELEGSDTTPFEAIQYVQLIALTKALFQAYPLEHIVGHADIAPTR
                     KTDPGPFFDWHGFRESIRAFIPTTVTTLPGNERSN"
     gene            complement(3065..3964)
                     /locus_tag="PK522_07645"
     CDS             complement(3065..3964)
                     /locus_tag="PK522_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HNC41643.1"
                     /translation="MNITQLNQNFGIANTVKFIEGQGGLPFIQIHTDKAQALISVYAG
                     QVLSFKPVSQEADLMFLSSKAYYQAGKAIKGGVPICWPWFGADPEGKGRPAHGFVRNR
                     MWDVIETDLNSKGEVKVTLGLNDTPETQAIWPYSFQLRLVITISETLGLELITHNTGN
                     QAFTITQAFHTYFGVKDIHRATVTGLDGKSYLDKVDAGQQKQQSGDVKISSEVDRIYL
                     DVSNELTIHDAAMNRQIIIRSQGSQTAVVWNPWEKIAKEMADLLDDDYLRLLCVETTN
                     AAADTILIQPGSQFSLIAHYQVK"
     gene            4074..6788
                     /gene="acnA"
                     /locus_tag="PK522_07650"
     CDS             4074..6788
                     /gene="acnA"
                     /locus_tag="PK522_07650"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HNC41644.1"
                     /translation="MNHNLFNSLKTLQFPGKSHQYYALSALEEAGLGNISRLPISIRI
                     VLESVLRNFDDKKITESHIKQLAGWEPNAARIFEIPFVVARIVLQDFTGVPLLVDLAA
                     MRSAAMRLGKDPNRIEPLVPVDLVVDHSVQVDYYGDKQALARNMEIEFERNKERYQFI
                     KWGMQAFDTFKVVPPGIGIVHQVNLEYLARGVHLKQGLIYPDTLVGTDSHTTMINSIG
                     VVGWGVGGIEAEAGMLGQPVYFLTPDVVGVNLTGQLREGVTATDLVLTITEMLRKAKV
                     VGKFVEFYGEGTASLSLPDRATIANMAPEYGATMGFFPVDDATIEYFSHTGRTAEEIQ
                     AFTSYFKAQGLYGIPQQGDIDYSQELHLDLGTIVPSLAGPKRPQDRIPINQLKTTFST
                     LFSKSITENGYGKSPELLAQGFPTIHKALNQPVELHHGDVLIAAITSCTNTSNPSVLI
                     AAGLLAKKAIEKGLSVKRHIKTSLAPGSRVVTAYLTAAGLLPYLERLGFNIAGYGCTT
                     CIGNSGPLPESIEESIIKEDLICSAVLSGNRNFEARVHANIRANFLASPPLVVAYAIA
                     GTVLKDLTTEPIGIGNGNQPVWLRDIWPTSAEIHELMKYAMQADTFRELYRDLTKDHT
                     LWNNITAVSGKLYDWPESTYIAEPPFFQDFSLQPTQTGRSAEITGARALAFFGDSVTT
                     DHISPAGAIKDTSPAGKYLLDHHVTKADFNSYGSRRGNHHIMMRGTFANVRIKNLMIP
                     GSEGGITLFRQAKSNECQQMSIYDAAMRYMEQATPTLLFAGKEYGTGSSRDWAAKGTQ
                     LLGVKAVIAESYERIHRSNLVGMGVLPLQLKENESVQTLSITGDEFFDIKGIETIQPQ
                     QTVTLVIHRPNGEQSQVQLICRIDTAIEVEYYYQGGILPYVLRQLLNH"
     gene            7241..8206
                     /locus_tag="PK522_07655"
     CDS             7241..8206
                     /locus_tag="PK522_07655"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNC41645.1"
                     /translation="MKKIRILLVALLMFFSPIGSVWSLPFTSMYVFGDSLSDGGSSPS
                     AVLSIYKLLGGNCDPSHPCPPYFEGRYSNGAVAAEYLAQHILPGGTSTSHFFNYAVSG
                     ATTGIGNFGDNGTTTTPGSFGLPGMNQEIGLYLSNVNGIADSQALYFIWGGANDFLTG
                     NAPDQAAQNIANYVSTLISAGAESFIIPNLPDLSLTPFAQIAGIETIAHAFSVAFNAQ
                     LATLLDNLALQFPFANIVQFDISAVLMEAIQHPSQFGFNDAQNACLLVMCSNPETFVF
                     WDDFHPTTHAHAVFASLFANALPVPATLLLVIIGLPLIRLFRSSR"
     gene            complement(8203..>8786)
                     /gene="corA"
                     /locus_tag="PK522_07660"
     CDS             complement(8203..>8786)
                     /gene="corA"
                     /locus_tag="PK522_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNC41646.1"
                     /translation="GKIRHRGTDYLAYCLLDALVDNYFIILEKIGDSIDALEEALMLQ
                     PGQEVMEQLYQLKRELLFLHKAVWPLREVISFLLRRESSLIQDSTIPYLRDVYDHVVQ
                     AIDSIEINRDMLTTMLDLYLSSVSYRTNEIMKVLTIIATIFMPLTFLAGVYGMNFEHM
                     PELSWKYGYLLIWIIMIALGISMLYFFRRKKWI"
BASE COUNT         2557 a         1960 c         1751 g         2518 t
ORIGIN      
        1 cccaaagata atcagaattc tcagctcgtg cataactcgg tcgaactggc atcgaatcaa
       61 cctgaaatac atgaattggg gtcaaccgaa ggcaacatta aacaaagtga gcaaactgaa
      121 gaaaaaagcc gtccacgccg acgtcgggga ggcaggcagc gtaatcggtc tgatcggcct
      181 agatcgaccg ctaccagtga aacgctagaa acgacttctc atgcagtaga tgcgatccct
      241 tcaatcgcta tcgcatcgac tgaacccttt gaaatagtag cacggtcgga tttcgaagca
      301 gtcaattcaa ctgatcatca gtctcaaccg atgaactctg atgcttgggc tgaacatcga
      361 ttacaagaag taccgtctga aaaaatcgaa cgcactgaag atcaagcttc ttctattaag
      421 gcagctaact cagttacgca agatgaactc tcagttgatt cagcatcttc atttaaaccg
      481 gtaacggtta ctgcaccagt ggtgatcgat ttacccgatt tatccaagag tgggctcgtt
      541 atgattgaaa ctactcagga aaacgtatca gaaattttag atgatgaaat gctattgact
      601 cgaaaaccgc gtcgaaaaac acaggcagca aaaatagacg ctccagaaac tgctgaaccg
      661 ctaatccaaa ttgaaactaa ggattagact cgcaaccgaa aagggaatag atatattccc
      721 ttttcaacac atatattcgc ttaatctaat ttgaaattcg agaacaggtt tttatataag
      781 cgaatagcaa ttcctcaaga aatagtcgcg catttaaaga atgccttgac cattgttgaa
      841 actgagctaa tcgcttccag aaatcgataa gttgttttgg ctcagttgtt gccacgattt
      901 ttttgatggc tgcttcatac cgatgatgat agtaaaccct acctagcatc tgaaaactta
      961 ctaagtcata acaccatttt tgtaaccaac taacgatcat tgataattcc tgcttatgga
     1021 gcttctctgc caatgtgatc ggatcaagct gcatcggtga actgattttc tcaatcaatt
     1081 ctacgtaata ggcagaataa cctgcttcga gatagcgttt caccaattga ggcgaagtac
     1141 caaaccaact aagatcgatt gaattctctt caatcccttc ttggtgtaac caattcttaa
     1201 ctgatagtct atccgggtga ggcaaagtaa ttctcatgca tcgactaaca attgttggaa
     1261 ttaatgcttg taaacgatca gtcaccagaa aaaacaaaac ctgcggagga ggttcttcaa
     1321 ttcgtttcag catcgcattg gctgctgaaa cattcataca atcggctgga tggattaata
     1381 aaatcttacg ccctgattga tgccctgata aataaagaaa ttgatcgaga tggcgaattt
     1441 gatcaatcgt aatttgctga ctcaatttct tttttgaatt tgtatcttca cttgattgtt
     1501 tagaatcact gagtgcctgt gttgcatcga gatcggattc aaccaacaat gcttcaggtt
     1561 ctaaacgata aaaattagga tgagttcctt gttcaaacca ttgacaactc ctgcagctac
     1621 cacaagcaaa ttgcgcttgg gttggttgtt cgcataacag tgattgagca agatactcgg
     1681 aaaactcaag ctgacctatc ccttttcttc cactgaatat gaaagcttgg gccttcgtaa
     1741 tcacatgatt tcgaatctga ggccaaactg attgttgcca aggataaagt tgattcatgg
     1801 tttctgctca atcaacaaag gctgaagtac tttttccaac tgatgagtga tgtcatcgat
     1861 cgatcgactc gcatcaagca cttgcattcg ctccggaaat tgctttgctc tatttatgta
     1921 tgctgctctt acccgatgaa agaaatcatt ttgttctttc tcaaaccggt ctgcgatttt
     1981 gatggattgc atccgaagtc gtgccacctc gatcgataaa tcaaaataaa acgtcaaatc
     2041 aggttgaaat tgaccctgaa cccaattttc caaaatttgt aacttatcga ttgaaacgtc
     2101 tcttcccccc ccttggtaag cataactcgc atcggtaaat cgatctgata tcacccaatt
     2161 cccttgatct aaagcaggga gaatcaattt atccaaatgt tcgcgtcgtg ctgcaaacat
     2221 gagtaaagcc tcggtttcag catgcattat ctgacggtga tctaataaca aaccacgaat
     2281 gctttcacct aactcggttc cacccggctc gcgcgttgtc aatacacgaa tacccttatt
     2341 cgttagataa tttgctatcc atttaagatg agtactcttc cctgccccat caataccttc
     2401 taatgtaata aatttacctt tttgcatgcg caaacgaaca atattaaatt atgcttaact
     2461 tgaactatga tatgacttgt tccatcaaca taaaacaatg cttaatcgat gaaaatcaat
     2521 acagcaggtt acctgcaaaa cgcaattcag attccatctc ctaattttga tgagcgacca
     2581 ctcggcacca ccatacgttt attagtcatt cacaatatta gcctaccccc caatgtcttt
     2641 gaaggaaatg ccatcatcga attgtttacc aatcagctcg attgcaatac tcatccttat
     2701 tacgaaggat tacgcgactt gaaggtttca agtcatttcc tcatccgtcg ccaaggcgaa
     2761 atcatacagt ttgtttcctg cattaaacgg gcctggcatg ccggtcaatc gtgttggcaa
     2821 ggggaagaac gctgcaatga tttctccgtt gggatcgaac tagaaggatc cgatacgacc
     2881 ccttttgaag cgattcaata tgtccagtta attgccttaa caaaagcatt atttcaggct
     2941 tatccccttg aacatattgt gggtcacgct gatattgcgc caactcgcaa aaccgatccc
     3001 ggcccattct ttgactggca tggctttcga gaatccattc gagctttcat tccgacaacg
     3061 gtgactactt tacctggtaa tgagcgatca aactaaactg actcccaggt tgaatcaaaa
     3121 tagtatccgc tgctgcattg gtcgtttcaa cgcaaagtag acgtagatag tcatcatcca
     3181 ataaatcagc catctcctta gcaatctttt cccaaggatt ccataccacc gcagtttggc
     3241 taccttgcga ccgaatgata atttgccgat tcatcgcggc atcgtgaata gttagctcat
     3301 tactgacatc caaataaatc cgatcgacct ccgaactgat tttcacatct cccgattgtt
     3361 gtttttgttg acctgcatcg actttatcga gataggattt accatctagt ccagtcacgg
     3421 tcgcacgatg aatatccttc acaccgaaat aagtgtgaaa tgcctgcgta atggtgaatg
     3481 cctgattgcc ggtattatgg gtaattaact ccaagcccaa tgtttcgctg attgtaatca
     3541 ctaaccttag ttgaaatgaa tagggccaga ttgcctgtgt ttcaggggta tcatttaagc
     3601 caagcgtgac tttaacttca ccttttgaat taaggtcggt ttcgatgacg tcccacattc
     3661 gattccgaac aaatccatgg gcaggccgcc cttttccttc cggatcagca ccaaaccacg
     3721 gccagcatat aggaactccc cctttaatcg ctttcccagc ttggtagtaa gctttgctac
     3781 tcagaaacat taaatctgct tcctgactca ccggtttaaa ggaaagtact tggccagcat
     3841 agacagaaat gagtgcttga gctttatctg tatgaatctg aatgaacggt aatcccccct
     3901 gcccttcaat aaacttaact gtgtttgcga ttccaaaatt ctgattcaat tgcgtgatat
     3961 tcatcttttt ttaccttatt ttttgtagca aataggattg gaatgataaa ctcaaaacaa
     4021 gacatcataa actaggattg attactaaag ccatgctttg aggtacatac tgaatgaatc
     4081 acaatctttt taattcgctt aaaacactac aatttcccgg taaatcgcat caatattacg
     4141 ccctgtctgc ccttgaagaa gcgggattgg gtaacatttc gcgattacca atttcaattc
     4201 gtatcgtatt agaatccgta ctacgaaatt ttgatgacaa aaaaattacg gaatcccata
     4261 tcaagcaact cgctggatgg gaacccaacg cagcacgcat ttttgagatt ccattcgtag
     4321 tcgcacgcat tgttttacaa gattttacag gtgtaccgct gttagttgat ttggctgcga
     4381 tgcgcagtgc cgccatgcga ttaggcaaag atcccaatcg gatcgaacca ctggttccag
     4441 ttgatttagt ggttgaccat tccgttcagg ttgattatta cggtgataag caagcgcttg
     4501 ctcgtaatat ggaaatcgaa ttcgagcgca ataaggagcg ttatcaattc atcaaatggg
     4561 gtatgcaagc gtttgatact ttcaaagtag ttccgccggg tattggcatc gtgcatcaag
     4621 tcaatctgga ataccttgcg agaggagttc atctaaagca aggtttaatc tatccagata
     4681 cattagttgg tacagattca cacacgacga tgatcaacag tattggtgtg gtaggctggg
     4741 gagtcggggg tattgaggca gaagctggaa tgctgggtca acccgtctat ttcctgaccc
     4801 ccgatgtcgt tggggtcaac ttgacagggc agttacgtga aggtgtgacc gcaactgatt
     4861 tagtactcac cataacggaa atgcttcgca aagccaaagt cgttggtaag tttgtcgaat
     4921 tttatggaga aggtaccgct tcattaagtt tgccagaccg agcaacgatt gccaacatgg
     4981 caccagaata tggtgcaacg atgggttttt tccctgttga tgatgcaacc atcgagtatt
     5041 tcagtcatac cgggcgaact gccgaagaaa ttcaagcttt tacaagttat ttcaaagccc
     5101 aaggactcta tggcatcccc caacaaggag atatcgatta tagccaggaa cttcatttag
     5161 atttaggcac cattgtacct tcattggctg ggcctaagcg acctcaggat cgcattccca
     5221 ttaatcaact caaaaccact ttctcaacac tctttagtaa atcgattact gaaaatggtt
     5281 atggtaaatc ccctgaacta ctggcccagg gcttccccac aattcataaa gcgttgaatc
     5341 aaccggttga gttgcatcat ggcgatgttc ttattgctgc aattacctcg tgtactaata
     5401 cttcaaatcc tagcgtactg attgctgcag gattactcgc taaaaaagcc attgagaaag
     5461 gattatcagt taagcgccac attaaaactt cactcgcgcc cggatcaaga gtagtcactg
     5521 cttatcttac agctgctggg ttacttcctt atttggaacg actaggcttt aatatcgcag
     5581 gctacgggtg tacgacttgt attggcaatt ctggcccact ccctgaatcg attgaagaaa
     5641 gcatcatcaa agaagattta atctgctcgg ctgttttatc tggaaatcga aattttgaag
     5701 cgcgagtcca tgccaatata cgcgctaatt ttctagcttc tcccccattg gttgtcgctt
     5761 atgcaatcgc aggtaccgta ttaaaagacc ttaccactga gccgatcggt atcggtaatg
     5821 gtaatcaacc tgtctggcta cgtgatattt ggccaacgag tgctgaaatt catgaattaa
     5881 tgaaatatgc catgcaagcg gatactttcc gtgaactcta tcgtgattta acaaaagatc
     5941 acacactttg gaacaacatt actgctgtgt ccggaaagtt atacgactgg cccgaatcga
     6001 cctatatcgc cgagccgcct tttttccaag atttctcgct ccaaccgact caaaccggtc
     6061 gttctgctga aataacaggt gcacgcgcac tggcattctt tggtgactcc gtgacaaccg
     6121 atcacatcag ccctgctggc gcgattaaag atacttcgcc tgcaggtaag tatctattag
     6181 atcaccatgt taccaaagct gattttaata gctacggctc acgccgtggt aaccaccaca
     6241 ttatgatgcg cggcacattt gccaatgtaa gaattaaaaa tttgatgata ccgggtagcg
     6301 aaggtggtat taccttattt agacaggcga agtcgaatga gtgtcagcaa atgtcaatat
     6361 acgatgcggc catgcgctat atggagcaag caacccctac gctcctattt gctggtaagg
     6421 aatatggcac aggatccagt cgcgattggg cagctaaagg cacccaatta ctcggtgtta
     6481 aagcagttat tgcagaaagc tatgaacgta ttcatcgcag caacctcgtg ggtatgggcg
     6541 tactcccttt gcaacttaag gaaaatgaga gcgtccaaac gcttagcatt acgggagatg
     6601 aattctttga tattaaaggc attgaaacga ttcaacctca acaaacggtt acattggtca
     6661 tccaccgacc aaacggcgag caaagccaag ttcaattaat ttgtcgaatc gatacagcga
     6721 tagaggtgga atattactac caaggcggta tcctacctta tgtactcagg cagttactca
     6781 atcattagcg caagctagaa caacaaaatc gttgttaatt tataagaaat actactgagt
     6841 aaaatttaaa cagggggagg taaaaaaaag aggcatctta aagtcaggct aattgcttaa
     6901 actgttaaga tgcctttgag gaggaaggaa atagcattat taaacaaaag tcctctttac
     6961 tcattggtac ggtttttttc aaatcaatca aaagttaatt acctctatta atgtatttta
     7021 aaatggccgc ccatgaaatt catggcttgc tacatcatcg tgcaatctgc accaaattgt
     7081 caacagttta tcgaataaaa atctaactga ctgtgagaaa aatcacttaa ctaccgcata
     7141 atcagtgggt tatatttttt cttgcgaatt aacgattcat attgcatact gagtcttcag
     7201 aaaaaatggt atttatattt atcgttaacc aaagctcaat atgaaaaaaa tacgaattct
     7261 gctggttgct ttgttgatgt ttttttcacc cataggaagt gtttggtctc tacccttcac
     7321 aagcatgtat gtcttcggtg atagcctttc ggatggagga agtagtcctt cagccgtgtt
     7381 aagtatttat aagctattag gtggaaattg tgacccttcc catccatgcc ctccttattt
     7441 cgagggacga tactccaatg gcgcagttgc agctgaatat cttgcacaac acattcttcc
     7501 cggtggtact tcaacaagtc atttctttaa ttatgccgtt tcaggtgcaa cgacagggat
     7561 tggtaatttt ggtgacaatg gcacgacaac gacacccggt tcgtttgggt tacctggcat
     7621 gaatcaagaa atcgggttat atctctcaaa tgtaaatgga atcgccgatt cgcaagcttt
     7681 atattttatc tggggaggtg ccaacgattt tttaactggt aatgcacctg atcaagcagc
     7741 ccaaaatatt gcgaattacg tgagcacatt aatctccgct ggcgctgaat ctttcattat
     7801 ccccaatctt ccagatttga gcttaacgcc atttgcgcaa atagcgggga ttgaaaccat
     7861 cgcgcatgct ttttcagtcg catttaatgc gcagcttgct acgttattgg acaatttagc
     7921 gttgcaattt ccttttgcga atatcgtaca atttgacatc agtgcggtat tgatggaagc
     7981 gatacagcat cccagtcaat ttggatttaa tgatgcgcaa aatgcgtgct tactggtaat
     8041 gtgctcaaat cctgaaacct ttgtattctg ggatgatttt cacccaacga cgcatgcaca
     8101 cgcggttttt gcttccttgt ttgccaatgc gctcccggta ccggcaacat tactcttggt
     8161 cattatcgga ttaccgttaa ttcgcctatt tcgttcctcg cgctagatcc atttctttct
     8221 tctgaaaaaa taaagcatgg aaatgcctaa agcaatcatg atgatccata tcaacagata
     8281 accatatttc cagcttagtt cgggcatatg ctcgaaattc attccataga caccggctaa
     8341 aaaagtaagc ggcataaaaa tcgtcgcgat aatggtcagc actttcatga tttcattcgt
     8401 tcggtaactc acgctggaga ggtatagatc gagcatagtc gtgagcatat cgcgattaat
     8461 ctcgatacta tcgatcgctt gtaccacatg atcgtaaaca tcccgtaaat aaggtatggt
     8521 tgaatcttga atcaacgacg actctcgcct caataagaac gaaataactt cgcggagtgg
     8581 ccatacggct ttatggagga atagcaattc acgtttcaat tgatatagct gttccatgac
     8641 ttcctgaccg ggttgtagca ttaaggcttc ttcaagcgca tcgatggaat caccgatttt
     8701 ctccagaata ataaaataat tatcaaccag tgcatccaat aagcaataag ccaaatagtc
     8761 cgttccgcga tgtcgaattt tgcctt
//
LOCUS       DAOQLJ010000096         8778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3155_length_8778_cov_6.635561, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000096 DAOQLJ010000000
VERSION     DAOQLJ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8778
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3155_length_8778_cov_6.635561"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PK522_07665"
     CDS             <1..514
                     /locus_tag="PK522_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC41647.1"
                     /translation="TQQMMNTLRALPFGEALVFWQPNEIRSLSLKIFDRSFAVTYLLE
                     IIAVLVGLLGVAASFSAQVLTRIKEFGILRHMGLTRGQIIRMLTLEGGLLTGFGIAIG
                     FILGGSISLILIFIVNPQSFHWTMQLYMPWNWLALMIIALLFSAALTAYIVGKSAVAR
                     QAVQSVKEDW"
     gene            514..1578
                     /locus_tag="PK522_07670"
     CDS             514..1578
                     /locus_tag="PK522_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HNC41648.1"
                     /translation="MRLLLILLALIGLGIGVAAPPILLPVTPRELVFPEDFGAHENFR
                     IEWWYATGWLETTDQKTVGFQVTFFRPTTEYDQDNPSQFAPKRLIIGHAAISDPAVGR
                     LLHVEKTVREGFGLAYAKAGNTDVKLDDWYFIRDTDGSYRTVVQGNDFSLELRLEPSQ
                     PLMLQDQAGFSRKGPGSEQASYYYSEPHLKVTGKMVRAGNTTEVKGTAWLDHEWSSEY
                     LDPNAEGWDWTGINLDDGSALMAFQIRGKQGEKIWAYAGLRDASGRMTIFSPEEVSFH
                     PVRTWKSPHTGATYPVAMRIMTEETEWVLTPLMDDQELDSTQSTGAVYWEGAVTVIRN
                     GQPAGKGYLELTGYKKALSM"
     gene            complement(1653..2621)
                     /locus_tag="PK522_07675"
     CDS             complement(1653..2621)
                     /locus_tag="PK522_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41649.1"
                     /translation="MGIYYGDNSNNHLWEPYPKTESDKMFGFGGNDFIEGGYGNDSLY
                     GGSGNDQVNGDEGNDILYGESGIDTLLGGEGNDWLYGQSEIDRLEGGNGNDVLDGGTG
                     ADWMFGGLGDDYYYVDNVGDYIWETDGWDAVASEVYSYTLPTGVERLNLGENGVIGYG
                     NALNNLIFDYGNVGVSYLYGGYGNDTLWGYTGDDFLAGEWGNDTIYGGDNIDTIAGGA
                     NSDTLWGEGGADYFTYYDRSESINNSYGWDIIKDFNRWEGDKIDLSWLDANLNKSGSQ
                     AFTVDHFNYYGWGNNGYLEVYTIGGSTMLIELAGAPQLNIATDIIM"
     gene            2711..2872
                     /locus_tag="PK522_07680"
     CDS             2711..2872
                     /locus_tag="PK522_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41650.1"
                     /translation="MKNLTIQMNGGNEHVLFENRLPWNTEYSREYLPNITRLEKGERN
                     GFYARHMII"
     gene            complement(3225..4367)
                     /locus_tag="PK522_07685"
     CDS             complement(3225..4367)
                     /locus_tag="PK522_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNC41651.1"
                     /translation="MVIQLEQYSAGHYEKGYEYRYFLPSKINYEWVWQTSHINSLLEK
                     AAVKLGELNSFARLVPNIDLFIQSHVTKEAVVSSRIEGTQTNMREAILQEADVSPEHR
                     NDWREVQNYIAALNQAIGELESLPLSSRLLKKTHAILLDSVRGKHKLLGEFRTSQNWI
                     GGNSLADATFIPPHHQYVNDLMGDLENFLHNHDIHVPALIRIAIAHYQFETIHPFLDG
                     NGRIGRLLITLFLVSEGVLEKPLLYLSSYFEKNKSLYYDNLMLVREKNNMLQWFKYFL
                     VGIEQTATLAVNTLSNILGLKAEIEQSLRTKLGRRSANAQILLNALFKHPVTDVEQAQ
                     KVCNLSYKAANDLIADMCKHGILIEITGQSRNRMFMFSSYIETFNE"
     gene            complement(4712..4787)
                     /locus_tag="PK522_07690"
     tRNA            complement(4712..4787)
                     /locus_tag="PK522_07690"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4752..4754),aa:Arg,seq:cct)
     gene            4823..6070
                     /locus_tag="PK522_07695"
     CDS             4823..6070
                     /locus_tag="PK522_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC41652.1"
                     /translation="MDKQPSSINQDRVHRIKLPQAVIVLGLVSFFNDVASDMVIPLIP
                     ILLASVLASGPIALGLIEGIADALASFLKLWSGRHTDVMGGRRKALAVVGYSLSNFTR
                     PLLGIAASWLSVLILRSIDRVGKGLRSAPRDALIADATPVEIRGYAFGFHRALDNSGA
                     VAGSLIAAAILAWSGLNLAEVILWSALPGAIAVVLFIVGVKETQQNTQRGDTNLVQIP
                     TTNLPPLRWSSLSSSTQRYLIVLVLFTFARASETFIILLGYQLGIEVVELLIIWAAIN
                     LSKALTSTLGGQWADRYGRGNVMLISWVAFAIAFLLLSFATNNIELWAISVFYGLFAG
                     LGEGAERALIVDYAHPKERGTAFGWYHLMTGLAAIPAGLLFGSLWQGYSAALAFVVAS
                     GLAVIASILLRLWAWPNYGRIIR"
     gene            6148..6519
                     /locus_tag="PK522_07700"
     CDS             6148..6519
                     /locus_tag="PK522_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNC41653.1"
                     /translation="MADFTEAQLSQLKSAMQKRYLALQEEIHNELEHVSDSRYIDLTS
                     GFSGIPDDIDTARVDRQVNEMRQLEEALKQLNDLNFGDCIDCTSEIGFERLLAYPAAQ
                     RCIKCQKHHDRMYRHEASPTF"
     gene            complement(6503..7147)
                     /locus_tag="PK522_07705"
     CDS             complement(6503..7147)
                     /locus_tag="PK522_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNC41654.1"
                     /translation="MAVWLHWTQKSISITSVPKDKTPHPPDNPISDDEMLAFQEAMRD
                     VTPLAAPNCVLPQPQQPEFVRSPANELPSTKAEWSDHIALSLAAGEEWSYLRPGLSRQ
                     ILRKLRRGQWKVSAELDMHGMTKDQARQALATFLLECKQFNIRCVCIIHGRGLSSKNN
                     EPILKSRVGNWLAQCEDVLAFCQAPPTLGGSGAVIVLLKSQRSDQPNATTKTWD"
     gene            complement(7092..8045)
                     /gene="trxB"
                     /locus_tag="PK522_07710"
     CDS             complement(7092..8045)
                     /gene="trxB"
                     /locus_tag="PK522_07710"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNC41655.1"
                     /translation="MTTKHSRLLILGSGPAGYTAAIYAARANLNPVIITGLAQGGQLM
                     TTTDVDNWPADVMGIQGPELMERFQKHAERFQTEIIFDHIHTAKLTEKPFTLIGDQGT
                     YTCDALIIATGASAKYLGMPSEEAYMGRGVSACATCDGFFYKGQDVAVIGGGNTAVEE
                     ALYLSNIARRVTVVHRRDKFRSEKILIDKLMNKATNGNVNLAFNHVLDEVIGDASGVT
                     GIRIKQVQDGTTQHLEVKGVFIAIGHQPNTDLFQGQLEMRNGYLVTKSGNEGNATATS
                     ITGIFAAGDVQDQIYRQAVTSAGSGCMAALDAEKYLDHLGA"
     gene            complement(8078..8734)
                     /locus_tag="PK522_07715"
     CDS             complement(8078..8734)
                     /locus_tag="PK522_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HNC41656.1"
                     /translation="MNILSRRKFLRLGLAGSAASITLAGNKAVSSLLPFTPVEAEGPF
                     YPVTPQEDKDEDLTRIKGHHTSAQGEPIYVTGQVMNHQGQPLANASVEIWQANAKGRY
                     RHPRDPNPAPIDNHFQGWAIVTTDVQGHFRFKTIKPGAYPASAQWIRPPHIHFKISKE
                     HYQELITQMYFPDEALNEADLLLQHKKPSERSLMIAKRGALLNAIEVFEFPIVLRTTI
                     "
BASE COUNT         2369 a         1867 c         1963 g         2579 t
ORIGIN      
        1 gacccaacaa atgatgaaca cattaagagc attgcccttt ggggaagcat tggtattttg
       61 gcaaccgaat gagattcgat cccttagctt aaaaatcttt gatcggagtt ttgcggttac
      121 ctacttgctt gagataattg cggtgctggt gggattgtta ggcgtggcag ccagtttttc
      181 tgcccaagtg ttaacaagaa ttaaggaatt cggaatactg cggcacatgg gtctaacacg
      241 cggccaaatt atccgcatgc tcacattgga aggtggtctc ttgaccggct ttggtatcgc
      301 aattggtttt attttggggg gtagtatcag cttgattctc attttcattg tcaacccaca
      361 atcatttcac tggacgatgc aactgtatat gccgtggaac tggcttgctc tcatgatcat
      421 tgctttattg ttttcagcgg cgctgaccgc ctacatcgtt ggaaaatctg cagtggcaag
      481 gcaggcagtg caatcagtca aggaagattg gtgatgcgtt tactgcttat cttgctcgca
      541 ttaattggat tggggattgg tgtggctgct ccaccgatat tattaccggt tacccctcgg
      601 gaattggttt ttccagaaga ttttggtgca catgaaaatt tccgcattga atggtggtat
      661 gcaacagggt ggttagaaac caccgatcaa aaaacagtcg gatttcaagt cactttcttt
      721 cgcccaacca ccgagtatga tcaagataat cccagtcaat ttgcaccgaa acggttgatc
      781 attggtcatg ctgcgatatc agatccagcg gtgggtaggt tattgcacgt tgaaaaaact
      841 gtgcgcgaag gttttggcct ggcctatgct aaagcgggta atacagatgt caaattagac
      901 gattggtatt ttatccgcga tacagacgga agttatcgta cggttgtgca gggaaatgat
      961 ttttccctgg agttgcggtt agaacccagt caacccctca tgttgcagga tcaagcaggg
     1021 ttttctcgga aaggccccgg atctgagcaa gcgagctatt attatagtga accacatttg
     1081 aaagtaaccg gtaaaatggt acgcgcgggt aacacgaccg aagtcaaagg aactgcctgg
     1141 ctcgaccatg aatggtcatc cgaatatctt gacccaaatg cagaaggatg ggattggacc
     1201 gggattaatc tcgatgatgg ttctgcgttg atggccttcc aaattcgtgg caagcagggc
     1261 gagaaaattt gggcttatgc aggactacgt gatgcctcag gccgcatgac gattttctca
     1321 cctgaagaag ttagcttcca tccagtaaga acttggaaat ctccgcatac cggtgcaacc
     1381 tatccggtcg cgatgcgaat catgacggaa gaaaccgaat gggtactgac accgctgatg
     1441 gatgaccagg aactggattc cacgcaatcc actggggcag tttactggga aggtgcggta
     1501 acagttattc gcaacggaca acctgctgga aaaggttatc tagaattaac agggtataaa
     1561 aaagcattat cgatgtgatc gctcgtcgat ctgatcacta tttaaacaag acccctgatt
     1621 tatcatgatc aaaacaggga tctttttccg gactacataa taatatcggt agctatattt
     1681 aattgtggcg ctcctgctag ctcgattaac atggttgacc caccgatggt atatacttca
     1741 agatagccgt tgttacccca accataataa ttaaaatgat ctacggtgaa tgcttggcta
     1801 cctgatttat tcaggtttgc atctaaccag gatagatcta tcttatcacc ttcccaacga
     1861 ttgaaatctt tgatgatatc ccagccatag gaattattga tactctcact tcggtcgtag
     1921 taggtaaagt aatcagcacc tccttccccc caaagggtat cagaattagc gccacccgcg
     1981 atggtatcga tattatcccc cccgtaaatg gtatcattac cccattctcc agcgagaaaa
     2041 tcatctccgg tataacccca aagcgtatca ttcccgtacc cgccatacag gtaactgacc
     2101 ccaacattac cataatcaaa aataaggtta ttcaaagcat ttccatagcc gataacccca
     2161 ttttctccta gattcaatct ctcgacaccg gtcggtaaag tgtaggaata aacttcgcta
     2221 gcgaccgcat cccagccatc ggtttcccag atatagtcac ctacattatc cacataataa
     2281 taatcatcac ccaaaccgcc gaacatccaa tcggctccgg tgcccccgtc taatacgtca
     2341 tttccattac ccccttcaag tcgatcaatt tcactttgtc catatagcca atcatttcct
     2401 tctccaccca ggagtgtatc tatacccgac tcgccatata agatgtcatt gccctcatca
     2461 ccgtttactt gatcatttcc tgaaccacca tatagggagt cattaccgta accaccttca
     2521 ataaaatcat taccaccaaa accgaacatt ttgtcgctct cagtcttagg ataaggttcc
     2581 caaagatgat tattactatt atcgccataa tatataccca tttttttctc cttttaacga
     2641 ttagaatcag aatcttgttt gctatcaatc gatagtgttt aaagattaag taaaagcctt
     2701 aagaaaatat gtgaaaaatc tcacaattca aatgaacggc gggaacgaac atgttttgtt
     2761 tgagaatcgt ttaccttgga atactgagta ttcaagggag tatttaccga atataacgag
     2821 gcttgagaaa ggtgaaagaa atgggtttta tgcaagacac atgattattt gaatgtaaat
     2881 caattccgca gtaatgttgc atgtcagtct cctcatcgtt attggagcag tcagtttaac
     2941 aactgactgc gaggaagctg gctagaggat tatcagattc gatttttatt aaaaaactac
     3001 tgtcttcact tgatccagat gggtggctgg tgcgatttat atcaccaaaa tatgaggcaa
     3061 ccggtgtgag gaattcagca aaaatgtttt gtaatggtgt tattaaagat taacctatcg
     3121 gcgtatggca agaactttgt tcgaactatt gactcaggac agctactggg tgacctcaat
     3181 ttatgtttga attgaatata aggggagtat aaccaaaagt tttcttactc gttaaaagtt
     3241 tctatatagg aagaaaacat aaacatacgg tttcgactct gccctgttat ttctattaat
     3301 attccatgct tgcacatatc agcaatgaga tcgtttgcag ctttataact taaattacaa
     3361 actttttgcg cctgttcaac atctgtaact ggatgtttga ataatgcatt taacaatatt
     3421 tgtgcgttgg cgctccttcg ccccaattta gttcgcaaac tttgctctat ctcagctttc
     3481 aatcctagta tattggataa ggtatttact gctaacgtcg ctgtttgttc aataccaacc
     3541 aaaaaatatt taaaccattg cagcatgtta tttttttctc gtactaacat caggttatca
     3601 taatataggc ttttattctt ctcaaaatag ctcgagagat aaagcaatgg cttttccagt
     3661 acgccttcac tcaccaaaaa gagagtaata agtaagcgtc cgatacgtcc attaccatcc
     3721 aaaaatggat ggattgtctc aaattggtaa tgggcgatag ctatacgaat taatgctggt
     3781 acatgaatat cgtgattatg caaaaaattt tcaagatcac ccataagatc attcacatat
     3841 tgatggtgtg gtggtatgaa tgttgcgtct gccaggctgt tgccgcctat ccaattttga
     3901 ctggtacgaa attcacccag tagtttatgt ttaccacgca cactatctag taaaatagcg
     3961 tgtgttttct tcaataacct tgatgatagc ggtaaagatt ctaactcacc gatagcctga
     4021 ttaagtgctg cgatataatt ttgcacttca cgccaatcgt tacgatgctc cggcgaaaca
     4081 tccgcttctt gtaagattgc ctcccgcata ttggtttgtg taccctctat acgactagaa
     4141 acaaccgctt cttttgtgac atgcgattga ataaataaat caatattggg taccaaacgg
     4201 gcgaatgaat ttagttctcc taacttgaca gcagctttct caagtaggct gttaatatgc
     4261 gaagtttgcc atacccactc gtagtttatt ttagacggta gaaaataacg atattcgtaa
     4321 cctttttcat agtgcccggc actatattgc tcaagttgaa ttaccatgga tttataaaaa
     4381 ttaaagtaac atataaatgt tattatttta ctttaatcgt tagaagtaaa atataataaa
     4441 agttatttta ctttacgcat atttaagata cttaatgagt attggagcca aagtaataat
     4501 ataccccaag aaaattagcg aattattctc aaagtcacta tttttgaata atgcgcacgg
     4561 taagttttga atccacgaac atttagtttg aggtgaagta tctaatcgaa gtgaactagt
     4621 ggcgctgata tttttatgaa agagtctaca tgatttcgtg accccaacaa tagaccactt
     4681 gattagtatt tttggtaatg cactgatttt atggcgccct gaacacgaat cgaacgtgcg
     4741 acctacccct taggaggggg ttgctctatc cactgagcta ccagggcgta aaagaactct
     4801 cattttacgc tattatgagg agatggataa acaaccgtca tcgattaacc aagatcgtgt
     4861 tcaccgtata aaattgccac aagccgtcat tgtgctcggg ttagtgagtt tcttcaatga
     4921 tgtggcatct gatatggtga ttccactcat tcctatttta ttagcctcgg ttctagcttc
     4981 agggcctatc gcattaggtt tgatcgaagg tattgccgat gcactggcga gttttctcaa
     5041 gctttggtcg ggaaggcata ctgatgtaat gggtggtcgg cgtaaggcgc ttgccgtagt
     5101 tggttactcc ctatcgaatt tcactcgccc tttgctggga attgctgctt catggttatc
     5161 ggttttgata ctccgcagta tcgatcgggt tgggaaagga ttgcgcagtg caccccgcga
     5221 tgctttgatt gcggatgcaa cacccgttga gattcgtggc tacgcatttg gtttccatcg
     5281 cgcgctcgat aatagtggag cagttgcggg cagtctcatt gctgcagcca tattggcctg
     5341 gtcgggattg aatctggcag aggtaatctt atggtcagcg ctgccaggcg cgattgccgt
     5401 agtgttattt atcgtaggcg tcaaagaaac gcagcagaat actcagcgtg gcgatacaaa
     5461 tttagttcag atcccgacta cgaatttgcc gccgctgcgt tggtcatcat tatcatcatc
     5521 aacgcagcgt tatttgatag ttttagtact atttacattt gcacgcgcct cagaaacctt
     5581 catcatcttg ttaggatatc aactgggtat tgaggttgtc gagttgttga tcatttgggc
     5641 tgcgattaat ctgtccaagg cattgacatc aacgctggga gggcaatggg ctgatcgata
     5701 tggacgcggc aatgtgatgt taatcagttg ggtcgcgttt gcaattgcat ttttactgtt
     5761 gagctttgct actaataata tcgagttatg ggcgattagt gtgttttatg ggttatttgc
     5821 aggtctggga gagggtgctg agcgtgcctt gatcgttgat tatgcgcatc cgaaagaacg
     5881 tggaactgca tttggttggt accatctaat gactggattg gctgctattc cggctggctt
     5941 gctgttcggc tccttatggc aaggttatag tgcagcgctg gcttttgttg tggcaagcgg
     6001 tttggcagtg attgcatcga ttttgctgcg attatgggcc tggcctaact atggtaggat
     6061 aataagataa gtgagaaggc aggaagattc ttcccttcgt tgactaaacc taaaaaaaac
     6121 cgtgatgcgg gtaggagaaa gaatggcatg gctgatttta ccgaagcaca attaagtcaa
     6181 ttaaagagcg cgatgcaaaa gcgttatttg gctttacaag aagagattca taatgaactt
     6241 gagcatgtgt ccgattctcg ttatatagat ctaaccagtg gtttctctgg aatacccgat
     6301 gatattgata cggcgcgtgt cgatcggcaa gtcaatgaga tgcgtcagct tgaggaagcc
     6361 ttgaagcagc tcaacgacct taattttgga gattgcattg attgtaccag tgaaattggt
     6421 tttgaacgct tattagccta tcctgcagca cagcgttgca ttaagtgcca gaagcatcac
     6481 gaccgcatgt atcggcacga ggctagtccc acgttttagt agtagcattg ggttgatcgg
     6541 atcgctgcga tttaagcaaa acaatgacgg ccccactacc acccaaagta ggaggagcct
     6601 ggcagaatgc gagaacatcc tcacactgtg ccagccaatt accaactcgg gatttcagta
     6661 tgggctcatt attttttgaa ctgagccctc taccgtgaat gatacacaca catcgaatat
     6721 taaactgctt acattcgagc aaaaatgtag ccaaggcctg tcttgcttga tcttttgtca
     6781 taccatgcat atcaagttca gcgctgactt tccattgccc acggcgtaat ttacgtaaaa
     6841 tttgacgtga taagccggga cgcagataag accattcctc acctgcagcg aggcttagcg
     6901 caatatgatc tgaccactct gctttggttg aaggtaactc attagcaggg ctgcggacaa
     6961 attcaggttg ctgaggttgg ggtagcacgc aattcggcgc tgcaagcgga gtgacgtctc
     7021 gcatcgcctc ctgaaatgca agcatttcat cgtcactgat tggattatca ggagggtggg
     7081 gtgtcttgtc tttaggcacc gaggtgatcg agatactttt ctgcgtccaa tgcagccata
     7141 cagccactac ctgcactcgt tactgcctgg cggtagattt gatcttgcac atcgcctgct
     7201 gcaaatatgc ccgtaatgct ggttgcagtg gcgttaccct cattgccgct tttggtgact
     7261 aaatagccat ttctcatttc taattgacct tgaaagagat cggtattggg ttggtggccg
     7321 atggcaatga atacgccttt gacttctaaa tgctgagttg ttccgtcctg aacttgtttg
     7381 atgcgaatgc ccgtaacacc ggaagcatca ccgatgactt catctaatac atgattgaac
     7441 gctaagttca cattaccatt ggtggcttta ttcatgagtt tatcgatcag tattttttcg
     7501 gatcggaatt tatcacgccg gtgaacaact gtaacacgtc gcgcaatatt ggaaagatat
     7561 aacgcttctt caacagcggt attgccacca ccgatgaccg ctacatcttg ccccttatag
     7621 aaaaagccat cacaggttgc gcaagcagaa acgccgcggc ccatataagc ttcttccgaa
     7681 ggcatgccta gatattttgc ggaagcacct gtggcaatga tcaaagcatc acaagtatag
     7741 gtaccttgat caccgatcag cgtaaacggt ttctcggtca atttagcggt atggatatga
     7801 tcgaaaatga tttctgtctg gaagcgctct gcatgctttt gaaaacgctc catgagttca
     7861 ggaccttgta tacccatcac atcagcaggc cagttatcga catcggtagt cgtcatcagt
     7921 tgaccgcctt gggccaggcc cgtaatgatc acagggttga gattggcgcg tgcagcgtag
     7981 atcgcagcgg tgtatccggc aggaccagaa ccgagaataa gtagtcggga atgtttagtc
     8041 gtcatgattt aaatgagtcg atttaataat taaaaaatta gattgtagtt cttaatacaa
     8101 tcgggaactc aaaaacttcg attgcgttaa gcagtgcgcc tcttttggca atcatcaatg
     8161 agcgttcaga aggttttttg tgttgcaaca gtaaatcagc ttcattaagc gcttcatcag
     8221 gaaaatacat ttgtgtgatc aattcctgat aatgttcctt cgaaattttg aagtgaatat
     8281 gaggcggtcg gatccactgc gctgatgccg ggtatgcacc cggtttgatg gttttgaatc
     8341 gaaaatgacc ttgcacatca gtcgtgacga tagcccagcc ctgaaaatgg ttatcgatcg
     8401 gtgctggatt aggatcgcga ggatgacggt aacggccttt tgcattcgct tgccagattt
     8461 caacgctcgc attcgcaagc ggttgcccct ggtgattcat gacctgtcca gttacataaa
     8521 tcggttcacc ctgtgctgag gtgtgatggc cttttatccg agtgaggtct tcatctttgt
     8581 cttcttgagg tgtaaccgga taaaaggggc cctcggcttc taccggtgta aaaggtaata
     8641 atgaactaac agccttattt ccagcaagtg ttatacttgc tgcgctacca gctaacccga
     8701 gtcttaggaa ttttcgcctc gatagaatgt tcatgcgctt atttcctatc gtttacattt
     8761 gttcacaatg ctggtgac
//
LOCUS       DAOQLJ010000097         8745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3177_length_8745_cov_5.288608, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000097 DAOQLJ010000000
VERSION     DAOQLJ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8745
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3177_length_8745_cov_5.288608"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2420
                     /locus_tag="PK522_07720"
     CDS             <1..2420
                     /locus_tag="PK522_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNC41657.1"
                     /translation="EMREGRAKFKIEAGELIPANWFGHIKQKMRSFTIKSRINVISCL
                     AIVSLSFMGGWSLLGDYQSKERLRDVYENRTLVLIDLSTILDAIQRMRVNALTAANSN
                     NPVIAKERFADTNQLDRIFDENWSRYSVTSVTTEEKVKAEEFYRNVEAYRISRDKTMN
                     LSIEGDFEAARENAVKYARPKYDLVHAALVDLFEIQEKQTKVVFDTAIEQFEILKIST
                     IAMILFFTFIYLYLTRLLHRAINLPLASVSAIFRKIAAGDYTNQVAIVNHDEIGCLTE
                     SVQMMQTRAGAEIDEIKRVAAENRRIRNALDQSNASIMIVDPNSCIIYVNQAMQILLK
                     NNEKEFVAVLAGVQINAILGQSFTIFNEKLDLSEINKSTKQLLKIGSSIFVLTLTPVF
                     DDNGSRLGTSIEWLDRTAEAAVEDEVAQIVKSASEGDFSKRLETKSKNGFFEQLAKNI
                     NQLLENNEKGLTDIARMLGAMAKGDLTQTITRQYKGMFEKLKTDANATVQQLTDIIRQ
                     IKESADSINITSHEIAQGNTDLSQRTEQQSISLDNTASSMEELTATVKQNAENAKQAN
                     QLAVSASDVAIRGGEVVGQVVETMSSINESSKKIVDIIGVIDGIAFQTNILALNAAVE
                     AARAGEQGRGFAVVATEVRTLAQRSAAAAKEIKSLIGDSVNKVENGTQLVDQAGRTMD
                     EIVMSVKRVTDIMAEISAASQEQSEGIEKVNATITQMDEITQQNTALVEEGAAASESM
                     YELANELTKLVAVFRTNSSISLIAEAETESGQIERRGPHRATNVQRLPLSAKLVTNSI
                     EDTVAVT"
     gene            2497..3354
                     /locus_tag="PK522_07725"
     CDS             2497..3354
                     /locus_tag="PK522_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNC41658.1"
                     /translation="MNLDPVIDQPMREYDFTNQDFACVKRLIYQRAGISLAPSRSNMV
                     YTRLVKRLRETGYSQFKDYLAMLEENSDSGEWESFVNALTTNLTSFFREAHHFEMLTE
                     HLKRRKPGQTIKIWCSASSTGEEPYSLAMTAVETFDTYTPPVRILATDIDTNVLATAQ
                     AGIYSMNQLENFSDARLRRFFLKGKGSNAAFARIRPELKNLVAFKQLNLLDRSWPLKG
                     PFDAIFCRNVMIYFDKPTQHQLLDKFAPLLATDGLLFVGHSESFQYNENPFKLLGKTV
                     YELINKHSR"
     gene            3351..3941
                     /gene="cheD"
                     /locus_tag="PK522_07730"
     CDS             3351..3941
                     /gene="cheD"
                     /locus_tag="PK522_07730"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HNC41659.1"
                     /translation="MTTIDSDHLATTAYYDPIHQIDAVKLLPGEYYVTQKDILLVTVL
                     GSCVAACIWDSLLRIGGMNHFMLPGDENSGSSARYGDFAMSLLIEQLLKLGARKENLK
                     AKVFGGGNVLPGFAVTSIGERNAEFTLDYLQHQGIPIIAHDLTDVFPRKIYFFPKTGR
                     ALVKKLHVKNNTIFERERSHLAEIKQGFALTRSVMS"
     gene            4003..5085
                     /locus_tag="PK522_07735"
     CDS             4003..5085
                     /locus_tag="PK522_07735"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNC41660.1"
                     /translation="MKKKISVLIIDDSALVRNLLSSIINSQEDMEVIGTAPDPLIARE
                     MIRRLNPDVLTLDIEMPKMDGLEFLEKLMRLRPMPVIMISTLTEKSAEATIEALALGA
                     VDFVSKPKVDVASELQLYREEIARKIRMAVSVQIKKPTPVSTDTNQTASSAFGANRIY
                     STEKLIIIGSSTGGTEALKDFLVNLPPHTPGILIAQHMPEAFTKSFATRLDSLCKISV
                     KEAQGGERVLPGHAFIAPGHSHLLLKRSGANYVTELSQSEPVNRHRPSVDVLFRSAAT
                     YAGKNAIGVILTGMGRDGAAGMLEMRKAGAYNFAQDEASCVVFGMPKAAIEVGATDEV
                     VPLKNMADRVLAKLSSFGMDKMNVRI"
     gene            complement(5111..8743)
                     /gene="metH"
                     /locus_tag="PK522_07740"
     CDS             complement(5111..8743)
                     /gene="metH"
                     /locus_tag="PK522_07740"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNC41661.1"
                     /translation="MIQSYKLTEADYRGTRFADYPHDLKGNNDLLTLTRPDIIRSIHS
                     AYLEAGADIIETNTFNSNAPSMADYHMEQLVYELNVAGARLAREEASAFERRNPEHPR
                     FVAGVIGPTTKTASISPDVNDPGFRSITFDQLVKDYSESIRGLVDGGVDILLVETIFD
                     TLNAKAALFAIDQFFEQHHIRLPIMISVTITDASGRNLSGQTPEAFWNAVQHAQPLSV
                     GINCALGAELMRPYVEELSNVANVYTSTHPNAGLPNPLSETGYDESPEYTASLIKEFA
                     QSGFVNIVGGCCGTTPAHIQAIAKAVEHIPPRKIPDLPKKLRLSGLEPLNIGDDSLFV
                     NVGERTNVTGSRAFARLILNDNYNEALNIARNQVENGAQVIDINMDEAMLDSQKAMVT
                     FLNLIAAEPDISRVPIMIDSSKWSVIEAGLKCIQGKAIINSISLKEGEAEFIQHARLA
                     KRYGAAVIVMAFDEKGQADTKARKIEICTRCYRILVDQVGFSPEDIIFDPNIFAVATG
                     IDEHNNYALDFIEATQAIKQSLPYVKVSGGVSNVSFSFRGNEPIREAIHTAFLYHAIQ
                     AGMTMGIVNAGQLGVYSDIPKELLEEVEDVLLNRRPDATERLVSFAENFKGQKKEQIE
                     DLSWRNEPVRQRLAHALVKGISTYIEEDTEIVRQEIADKGGRPIEVIEGPLMEGMNVV
                     GDLFGAGKMFLPQVVKSARVMKQAVAYLLPYIEAEKKSSGDNRPKGKIVIATVKGDVH
                     DIGKNIVTVVLQCNNYEVINMGVMVPSAQILETARRENADIIGLSGLITPSLEEMAHV
                     AKEMEREGFKIPLLIGGATTSRVHTAVKIAPHYSGVTVWVPDASRAVGVCSQLMSQDQ
                     QVAYKAEVKAEQEKIRAQQKNKKGPAQLLTLEEARNNAFKTDWRSYTPPSPSFIGIRT
                     LNHYPLESLVPYIDWTPFFQAWELSGRYPAILDDEVVGEAAKNLYRDAQAMLKKITDQ
                     KWLTANAVIGLFPANSVNFDDIEIYADRSRNKVLMTYHTLRQQTIKPAGRPNLALADF
                     IAPKETGIIDTIGLFAVSAGFGIDERVKAFEAAHDDYSAIMLKALADRLAEAFAEHMH
                     ARVRREFWGYIKDESLNNEQLIDEAYQGIRPAPGFPACPDHTEKGPLFNLLEAEKRTG
                     IIVTESFAMVPTAAVSGFYFAHPESDYFAVGKIGKDQVEDYARRKGWSLSEAERWLAP
                     ILGYER"
BASE COUNT         2466 a         1774 c         2048 g         2457 t
ORIGIN      
        1 gtgagatgcg tgaaggtaga gccaaattta agattgaagc tggagaattg ataccagcca
       61 actggttcgg ccatatcaag caaaagatgc gttcatttac cattaaatcc cgaatcaacg
      121 tcatcagttg tttggctatt gttagtttga gtttcatggg tggatggagt cttttagggg
      181 attatcaaag taaagagcgg ctacgcgatg tttatgaaaa ccgtacatta gtactaatag
      241 atttatcaac gattctagat gcaatacagc gcatgcgcgt caatgctttg acagccgcaa
      301 attctaataa tccagtaatt gcaaaggaac gatttgcaga tacgaatcaa ttggatcgca
      361 tctttgatga aaattggtca cgatatagtg ttacttcggt tacgacagaa gaaaaagtga
      421 aagctgaaga attttatcgg aatgtagaag cctatcgaat ttcacgtgac aagacgatga
      481 atttgtctat tgagggagac tttgaagctg ctagggaaaa tgcggttaaa tatgctcggc
      541 caaaatatga cttagtgcac gcggcgcttg ttgatttatt tgaaatacag gaaaaacaaa
      601 cgaaagtagt atttgatact gcgattgaac aatttgagat actcaaaatt tcaacgatcg
      661 ccatgatttt attttttaca ttcatttatt tgtacctcac cagactgctg catcgagcca
      721 ttaatctacc cttagcaagc gtaagtgcca tttttaggaa gattgctgca ggtgattata
      781 ccaatcaagt tgccattgtt aatcacgatg aaattgggtg cttaaccgag tcggttcaga
      841 tgatgcaaac gcgggcgggt gctgagatcg atgaaatcaa gcgagtggct gctgaaaatc
      901 gacgtatccg caatgccctg gatcaatcta atgcatcaat tatgatcgtc gatcccaata
      961 gctgcatcat ctatgtcaat caagcgatgc agatattact taaaaataat gaaaaggaat
     1021 ttgtggctgt attagctgga gtgcagatta atgcgatatt aggccaatca tttaccattt
     1081 ttaatgaaaa acttgatctt tctgaaatta ataaatcaac caagcaatta ttgaaaatcg
     1141 gtagcagcat ttttgtattg acgcttactc ccgtattcga tgacaacggg tcccggctag
     1201 gaacatccat cgagtggctt gatcggacag ctgaggcggc tgtggaagat gaagtagccc
     1261 agattgtaaa atcagcgagc gagggtgatt ttagcaagcg cctagaaaca aaaagtaaaa
     1321 atggtttctt tgaacaatta gccaaaaata ttaatcaatt actcgaaaat aatgagaaag
     1381 ggttaaccga tattgcacgg atgctaggtg cgatggctaa aggcgatttg actcaaacca
     1441 ttacccggca atataaaggg atgtttgaaa aattgaaaac tgatgcaaat gcgacggttc
     1501 aacaactgac ggacattatt cgacaaatca aggaatcggc tgattcgatc aatatcacct
     1561 cccatgagat tgcacagggt aatactgact tatcgcaacg gacagaacaa caatccatca
     1621 gtctcgataa tactgcttcc agtatggagg agttgaccgc aaccgtaaaa caaaatgcag
     1681 agaatgcaaa gcaagccaat cagctcgcgg tatcggcatc tgatgtggca atcagaggag
     1741 gggaggtggt ggggcaggta gtagaaacca tgagttcaat caatgaaagc tcgaagaaaa
     1801 ttgttgacat tattggtgtg atcgatggca ttgcgttcca aaccaatatt ttggcattga
     1861 atgctgccgt agaagcggcc agagcgggtg aacaaggtcg gggattcgca gtcgttgcaa
     1921 cggaagtgcg tactttagcc caacgttcag ccgccgctgc gaaagagatc aaatctttaa
     1981 ttggagactc ggtgaacaaa gtggaaaatg gcacccagtt ggttgatcag gccgggagaa
     2041 ccatggatga gatcgtaatg tcagtaaagc gtgtgactga catcatggct gagatatcag
     2101 cagcttctca agaacaaagc gagggtattg aaaaagtcaa tgcaacgatt actcaaatgg
     2161 atgagattac ccaacagaac accgctttag ttgaagaagg cgctgctgca tcggaatcga
     2221 tgtatgaatt ggcaaatgaa ctcaccaaac tcgttgccgt gttcagaacc aattcatcaa
     2281 tttcattgat tgctgaggct gagactgagt ctgggcaaat tgagcgcaga ggccctcatc
     2341 gagcgacgaa tgtacagcgg cttccactat cagcaaaatt agtgactaac tcgattgaag
     2401 atacggtggc agtaacttaa acgaaggtaa ttgccatcaa aaggctaaag acagtttagc
     2461 gtcatatcat ttaatttaac tcagtaatga ctacttatga atctcgatcc ggttatagat
     2521 caaccaatgc gtgagtatga ttttacaaat caggattttg cttgtgttaa gcggctaatt
     2581 tatcagcgtg ccggaatttc tctggcaccg agtcgcagta atatggttta tacccgcttg
     2641 gttaagcgat tgagagaaac cggttatagc cagtttaaag attatttagc gatgttggag
     2701 gaaaatagcg atagcggtga atgggaaagt tttgtcaatg cattgacgac aaacttgacc
     2761 tcatttttcc gagaagcgca tcactttgag atgttgacgg agcatcttaa acgaagaaaa
     2821 ccagggcaaa cgattaaaat ttggtgcagt gcttcatcaa caggagaaga gccttattcg
     2881 cttgcaatga ctgctgtgga aacgtttgat acgtacacgc cacccgtacg tattctagca
     2941 actgatattg ataccaatgt gttagcaaca gcccaagcag gaatttattc catgaaccaa
     3001 ctggaaaatt tttctgatgc ccgattgagg cgttttttcc ttaaaggaaa agggtctaat
     3061 gctgcttttg cccgtatccg ccctgagcta aaaaatctag tagcgtttaa acagcttaat
     3121 ttattagacc gatcttggcc gcttaaaggg cctttcgatg cgatattttg ccgcaatgtc
     3181 atgatttatt ttgataaacc aactcaacac caattactgg ataagtttgc accgttgctc
     3241 gctacggatg gattattgtt tgtaggtcat tcagagagtt ttcagtataa cgagaatcca
     3301 ttcaaactgc tgggcaaaac ggtttatgag ttgataaata aacattcacg atgaccacta
     3361 tcgatagcga tcaccttgct acaactgcct attatgatcc catccatcag attgatgcgg
     3421 taaaactatt accgggtgag tattacgtta cgcagaagga catattatta gtaacggtgt
     3481 taggttcctg tgtggcagct tgtatctggg atagcttatt gagaattggc gggatgaatc
     3541 atttcatgtt gccaggcgat gaaaatagtg gatcttcagc gcggtatggg gatttcgcaa
     3601 tgtcattgct catcgaacag ttactgaagc tcggtgcgcg taaagagaat ttgaaagcta
     3661 aagtatttgg gggcggtaac gtattgcctg gttttgcagt aaccagtatt ggtgagcgta
     3721 atgcggaatt tacccttgat tatcttcagc atcaaggaat acccattatt gcacacgatt
     3781 taaccgatgt ttttccacga aagatttatt tttttcccaa aacaggtcgt gcgttggtca
     3841 aaaagttaca tgtcaaaaat aatacgattt ttgagcgaga aaggagtcat ttagcagaaa
     3901 ttaagcaagg atttgcattg actcgttcag tgatgagtta aaaaaagaaa aagatgaata
     3961 agcttcattt tgtaatgaag taatttttcc aggaagggca tcatgaaaaa aaagattagt
     4021 gtgttgataa ttgatgattc tgctttagtt cgtaacctac tttctagcat cattaatagc
     4081 caggaagata tggaggtgat aggtacggcg cccgatccac tgatcgctcg tgaaatgatt
     4141 cgtcgattaa atccagatgt cttaacactg gatatcgaaa tgcctaagat ggatggcctc
     4201 gagtttctgg aaaaattgat gcgcttacgg ccaatgcccg ttatcatgat ctctacactg
     4261 accgagaaga gtgctgaagc aactatagaa gcattagcac tgggagctgt cgattttgtt
     4321 tccaagccga aagttgatgt cgcgagtgag ctacagctat atcgtgaaga aatcgcacgt
     4381 aaaattcgca tggcggtttc agtacaaata aaaaaaccaa cgcctgtatc gactgacaca
     4441 aatcaaacag cttcttctgc ctttggagct aatcgtatct attcaacgga aaagttaatt
     4501 atcataggtt cctctaccgg gggaactgaa gcattaaaag atttcttggt gaacttacca
     4561 ccgcatacac ccggtatatt gattgctcaa catatgcccg aagcgtttac taaatcattt
     4621 gcaactcgtt tggatagcct ttgcaagatt tcagttaaag aagctcaggg gggggagaga
     4681 gttctaccag gacatgcttt tattgctcct ggccattctc atctgctatt gaagcgcagt
     4741 ggcgcaaatt atgtgacgga gcttagccaa tcggagcctg tgaatcgtca tcgaccttcg
     4801 gtggatgttt tgtttcgctc agcagctacg tatgccggca aaaatgcgat tggagttatt
     4861 ctgacaggca tgggtagaga tggggctgcc gggatgctgg aaatgcgtaa agcaggtgct
     4921 tataactttg cccaggatga agcgagttgt gtggtattcg gtatgcccaa agccgcaatt
     4981 gaggtgggtg caaccgatga agttgtgccc ttgaaaaata tggccgatcg tgtactcgca
     5041 aaattaagca gctttggtat ggataaaatg aatgtacgga tttgaacctt gagtatatcc
     5101 atgctcggtt ttatcgctca taacctaaga ttggtgcgag ccatctctca gcttcactaa
     5161 gcgaccagcc ttttcgtctt gcatagtcct ctacttgatc ttttcctatt tttccaacgg
     5221 cgaaatagtc tgattcaggg tgggcgaaat aaaaaccaga aactgctgca gttgggacca
     5281 tcgcgaaaga ctccgtaacg ataataccgg ttcgcttttc tgcttcaagt aagttaaata
     5341 atggtccttt ttccgtatga tcagggcaag caggaaaacc cggtgcgggc cgaatgcctt
     5401 gataggcttc atcaatgagt tgttcgttgt tgagtgattc gtcctttata tatccccaga
     5461 attctcgtcg gactcgagcg tgcatatgtt cagcaaatgc ttctgctagg cggtctgcca
     5521 gcgctttgag cataatggca ctataatcat catgggctgc ttcaaaggct ttaacccgtt
     5581 catcgatgcc gaaaccagca ctgacggcaa ataagccgat tgtgtcaata ataccagttt
     5641 ctttgggagc gataaaatcg gctagtgcca aattaggacg acctgccggt ttgatcgttt
     5701 gctggcgtag cgtatggtaa gtcatgagca ctttattacg cgagcgatcg gcataaattt
     5761 cgatatcatc gaaattgaca gaattagcag gaaacaatcc tataactgcg ttagccgtca
     5821 accatttttg atccgtaatt tttttcagca tggcttgtgc atcgcggtat aaatttttag
     5881 cggcttcacc gacgacttcg tcatctaaga tggcaggata gcgacctgat aattcccagg
     5941 cttggaaaaa aggtgtccaa tcgatatagg gaaccaatga ttcgaggggg tagtgattta
     6001 aggttctaat cccgataaaa cttgggctgg gcggagtata actacgccag tctgttttga
     6061 aagcattgtt gcgcgcttct tcgagtgtga gcagttgagc tgggcctttt ttgtttttct
     6121 gttgtgctcg gattttttcc tgttccgcct taacttcagc tttataggca acttgctgat
     6181 cttgggacat caattgacta catacgccca cggctcgact ggcatcgggt acccagacag
     6241 ttaccccgct gtaatgggga gcgattttaa cggcagtatg aacccgagaa gttgttgctc
     6301 ctccaatcaa gagtggaatt ttaaagcctt cgcgctccat ctctttggca acatgcgcca
     6361 tttcttcgag cgaaggggta atgagtccag ataagccgat gatatcagcg ttttcccgac
     6421 gcgcggtttc caagatctgg gcactgggaa ccataacgcc catattgatt acttcgtagt
     6481 tattgcactg tagtacgacg gttacgatat ttttcccgat atcgtggaca tcgcctttga
     6541 ccgttgcaat gacaatcttt cctttaggac gattatcgcc cgaacttttt ttctcagctt
     6601 caatataggg gagtaaatac gcaaccgctt gtttcatgac tcgcgctgat ttaacaactt
     6661 gcggcaagaa catttttcca gcgccaaata agtcacccac gacattcata ccttccatga
     6721 gggggccttc gatgacttcg atggggcgtc ctcctttatc agcaatttct tgtctaacga
     6781 tttcagtatc ttcttcaatg taagtactga tacccttaac cagggcatgt gctaagcgtt
     6841 gacgaacggg ctcattgcgc caagaaagat cctcgatttg ctcttttttt tgccccttga
     6901 agttttctgc aaaactaacg agtcgttcgg ttgcatcagg acgccggttg agtagcacgt
     6961 cttctacttc ttccaatagt tctttgggga tatccgagta aacgccgagt tgacctgcgt
     7021 tgacaatacc catggtcatt cctgcttgaa ttgcgtgata tagaaatgct gtatggattg
     7081 cctctcgaat cggttcattt ccccgaaatg agaacgatac attggaaacc ccaccactta
     7141 ccttgacgta gggcagagac tgttttattg cttgggttgc ttcaatgaaa tcaagcgcat
     7201 agttgttgtg ttcatcgatt ccggtagcaa ctgcgaaaat attgggatca aagataatgt
     7261 cttcaggaga gaatccaact tgatcaacta aaattcgata gcatcgtgta cagatttcaa
     7321 ttttgcgtgc tttcgtatcg gcttgtcctt tctcatcaaa ggccatgaca atcacggcag
     7381 cgccataacg ttttgctaat cgggcatgtt ggataaattc tgcttcaccc tcttttaaac
     7441 taattgaatt gatgatcgcc ttgccttgaa tacatttcaa gccagcttcg atgactgacc
     7501 atttgctgga gtcaatcatg atcggtaccc ggctgatatc aggttcggct gctatcagat
     7561 tgaggaatgt caccattgct ttttgggagt ccagcatagc ctcatccatg ttgatatcaa
     7621 tcacttgagc gccattctcg acctgatttc gggcgatatt gagtgcctca ttgtagttat
     7681 cgtttaagat gagtcgcgca aatgcccttg atcctgttac gttcgtacgc tcaccgacat
     7741 tgacgaaaag tgagtcatcg ccgatattga gcggttccaa gccggataag cgtaactttt
     7801 tagggagatc aggtattttt ctgggtggaa tatgttctac cgctttagca atcgcttgaa
     7861 tatgggcagg cgtggtgccg cagcatcccc ccacgatatt aacaaaccca gattgggcga
     7921 attctttgat caagctagcc gtatattcgg gcgattcgtc atagcctgtt tcagataatg
     7981 gattaggaag tcctgcattg ggatgggtac tcgtataaac attggcaaca ttggaaagtt
     8041 cttccacata aggacgcatt agctcagcgc ccaatgcgca gttgataccc accgataatg
     8101 gttgggcatg ctgtaccgca ttccagaacg cctcaggtgt ttgtccagag agattgcggc
     8161 ccgatgcatc ggtaatggtt acggagatca tgatcgggag tcgaatgtgg tgttgctcaa
     8221 agaattgatc gatcgcaaat aacgctgctt tggcattcag cgtgtcgaaa attgtttcaa
     8281 ccagaagaat atcgacgcca ccatctacca acccccgaat agattccgaa tagtctttta
     8341 ctaattgatc gaaagtaatt gaacgaaaac ccgggtcatt gacatcgggt gaaatcgatg
     8401 ccgttttggt cgtcggccca atgacacctg cgacaaaacg agggtgctcg ggatttctac
     8461 gctcaaacgc tgaagcttct tcacgggcta atcttgcacc agcgacatta agttcataaa
     8521 ccaattgctc catatgataa tcagccatgg agggtgcgtt tgaattgaag gtattggttt
     8581 cgatgatatc ggcgccagcc tcaaggtaag cgctgtggat tgagcgaata atgtcaggtc
     8641 gcgtcagcgt tagtaaatca ttattccctt tgagatcatg ggggtaatct gcgaaacgtg
     8701 ttccacgata atctgcttca gttaatttgt agctctgaat catgg
//
LOCUS       DAOQLJ010000098         8650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3226_length_8650_cov_5.480861, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000098 DAOQLJ010000000
VERSION     DAOQLJ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8650
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3226_length_8650_cov_5.480861"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            401..1852
                     /locus_tag="PK522_07745"
     CDS             401..1852
                     /locus_tag="PK522_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41662.1"
                     /translation="MKVPFRSKTTYCAGILASLCFSQSVLADTLITHWNEAALQAIRV
                     THPGPPIVARALAITHTCMYDAWSAYDAKAKGTRLDDDLRRPDNEHTMANKSKAVSFA
                     AYRCLSDLFPSEVASFDGIMKSLGYNPADQSTDTNTAEGIGNVAAAAVIQYRHQDGAN
                     QLGDLNGGAPYSDYTGYTPVNSPSSIVDPNHWQPLQVGSNMQSFITPHWGLVEPYALK
                     SGDEYRKKIPAPANYHTEPARYRVQAQQVVDYAAHLTDEKKVIAEYWADGPLSELPPG
                     HWTLFATFVSKRDRHNLDQDVKMFFALTNAIFDASIVSWDAKRHFDYVRPVTAIHFLF
                     SGQQIPSWQGLIDGADWKPYQADSVVTPPFPEYFSGHSIFSAAGAETLKLFTKSDNFG
                     HSVVVPARSSRVEPGVVPASDLVLFWATFSDAADEAGISRRFGGIHFIDGDLEARKVG
                     RVVAQNAWKKTLKYFGDKDHKHDKDDDHGKGKK"
     gene            complement(1955..2308)
                     /locus_tag="PK522_07750"
     CDS             complement(1955..2308)
                     /locus_tag="PK522_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41663.1"
                     /translation="MKTLLPFACIYILLIAPTGFLMGQLFFGHFSIAASLAGSAGLIC
                     AFAGFGVIGGAIKARSIAFWSGLFALIGVAFDAADYYLNYAIPGNYYAWGLIGPYCCA
                     IIFVAYVSRSLMVVK"
     gene            2539..3279
                     /locus_tag="PK522_07755"
     CDS             2539..3279
                     /locus_tag="PK522_07755"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNC41664.1"
                     /translation="MEKIRLSKLMSERGICSRREADRFIELGWVFVDGERISELGIKI
                     FPTQKIALNKAAQAQQTDLVTILLNKPIGYVSGQPEPGYQPAVTLIKPENLFSPKQAM
                     QPAKRFQPWHLKKLAPAGRLDIDSQGLLVLTQDGRIAKQMIGENSRIEKEYLVRVEGV
                     LPVAKLALLNHGLSLDGQALKPAQVSWQNRDQLRFILNEGKKRQIRRMCELVGLKVTG
                     LKRVRIGKVKLGNLPEGQWRYLENGEKF"
     gene            3300..3524
                     /locus_tag="PK522_07760"
     CDS             3300..3524
                     /locus_tag="PK522_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2835 family protein"
                     /protein_id="HNC41665.1"
                     /translation="MHQRVRVVLKIPAYQLELYYQGVVDTVAAETTDGRIVHFPANVL
                     RSVVQSHGVYGTFELVFDENNKFVSISSTS"
     gene            3630..3932
                     /locus_tag="PK522_07765"
     CDS             3630..3932
                     /locus_tag="PK522_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNC41666.1"
                     /translation="MRVKLIVLNSVPVEVFVMDAISYTTARANLAKTMERVCNDHSPV
                     IITRKREASVVMISLEDYQAMEETVYLLRSPANARRLLESIAELEAGKGTVRQLAE"
     gene            3929..4183
                     /locus_tag="PK522_07770"
     CDS             3929..4183
                     /locus_tag="PK522_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HNC41667.1"
                     /translation="MNLIFSTQAWEDYLYWQKTDKSTVKRIHGLIKAIQQKPFEGIGK
                     PEPLKHGLAGYWSRRIDNEHRIVYKILNTDLFIAQLRYHY"
     gene            4196..4525
                     /locus_tag="PK522_07775"
     CDS             4196..4525
                     /locus_tag="PK522_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNC41668.1"
                     /translation="MNDIQQLTEILLKFRDERDWAQFHNAKDLALALNIEAAELLEVF
                     LWKAPEQANIESVKEELADVLTFALLLSEKYGLDVKQIVLEKLEKNALKYPVDKSKGS
                     AKKYTEL"
     gene            complement(4535..5683)
                     /locus_tag="PK522_07780"
     CDS             complement(4535..5683)
                     /locus_tag="PK522_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC41669.1"
                     /translation="MSNNPIIRWREAGEEKSARWQSEKSLQPPQQVKVIDDRMPADNA
                     YRLACEGTALLWRSDFQNAKQLLQAMARRIDKKRSTSKHLPAELSVADTFHRYRLMQS
                     QRAQILDKLLIPFQADHTIALPRAPDIRHACLEAYGPRNEPYVASLRELLGLIGAHEW
                     RKKGINIPVLGITIHPHYGVFSPVRNEYIQLIAQAALPKILTMQSIAFDIGTGTGVLA
                     VILAQRGVQRIVATDHDPRALDCARDNLLRLGLIEKLELLQTDLFPQGQAALIVCNPP
                     WIPARPASPMEHAIFDPENRMLRGFLQGLRDHLLPGGEGWLILSDIAEHLGLRSRAEL
                     LHMIDTAGLKIISKSDVKPHHPRVSDTSDPLHTARTRELTSLWQLAAK"
     gene            complement(5842..6270)
                     /locus_tag="PK522_07785"
     CDS             complement(5842..6270)
                     /locus_tag="PK522_07785"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNC41670.1"
                     /translation="MPTNSVTSDLSLHVPLPESQPVLRMVPMPSNTNYAGDIFGGWIM
                     SQVDIAGSIPALRRARGRVATIAVNSFVFKQPVLVGDLVSFYADIIKIGRTSITVDVA
                     VYAQRGVREGGEEICIKVTEAVLTYVAIDTDRHPRPVPPE"
     gene            complement(6300..8108)
                     /locus_tag="PK522_07790"
     CDS             complement(6300..8108)
                     /locus_tag="PK522_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNC41671.1"
                     /translation="MHKDVIPIEAAQTLDGLFSERVTRSPQKIAYRDYNSVTEQWRDY
                     SWADMARWVVRWQAALTREDLSPGDRVAIMCRNCPQWVAFEQAALGLGLVVVPLYTED
                     RAENAAYCIENAGVKLLVVEKSTQWEAFLPIIAQLSSVLRIVILLVTDDATKRGYDPR
                     VVTLQQWLPEQGEQMLHINTNPDALATIIYTSGTTGRPKGVMLSHDNILSNTFASTQA
                     VDVLPDDLFLSFLPLSHTFERTSGYYVPMMCGATVAYARSIQLLQEDLLTIQPTVLVS
                     VPRIYERVYAGLRTKLAEGSVLARKLFYFAVSVGYSRFEQQQGRDTWHFSHLFWPILE
                     KLVARKLMDKLGGRLRLAMSGGAALSPEISRIFIGLGLPILQGYGMTESSPVVCVNRL
                     DDNLPSSIGRTISGVEVKLGENNALLIRGPNVMLGYWNNPEATKAVISEDGWLNSGDI
                     AQIDEQGRVTITGRLKDIIVTSTGEKVPPADMEAAILRDPIFEQVMVVGEGRSYLGAL
                     VVLSQRGLKTLAELCHLPPDPHQLINQESVEKILLDRIAQQIHEFPGYAKIFKVALIP
                     EPWSVANEMLTPTLKLKRSKIAQRYQAEIENLYTKR"
     gene            complement(8167..>8650)
                     /locus_tag="PK522_07795"
     CDS             complement(8167..>8650)
                     /locus_tag="PK522_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1974 domain-containing protein"
                     /protein_id="HNC41672.1"
                     /translation="PLLHWSIQDALYRTQQAFDEFLSNFPGHFLIRIWLRWQVFPLGK
                     RCSPPSDELSHRIARLILEPGEVRERLTAGIYVPTDDNEPLTQLEQALSCAINCEPIE
                     ARLRAATKEGQLTAKGDGKIAQAVALNLISAEEAALLDKMKILRRRVIMVDDFTPSFD
                     "
BASE COUNT         2356 a         1863 c         2084 g         2347 t
ORIGIN      
        1 gatacgatca ggtgatcgta tgtactagct aacaactcgc tcctgaatct ctgaagagag
       61 ctcatttgtt tttttattac gctatccact caaaaaatag cgcttcctgc aattcatact
      121 actttctctt ggaaagtagt atgtaccaat gaccaataga tgaagtgaca gcctttcgac
      181 agaattcaat gtgttgatta ctgatggcag tcagcctttg tataagcacc ccggaagttt
      241 tttacaaagg atcgacgcat gtgatttttc acacagattt tatcaatctt aatttgtaag
      301 attttcaccg gtagcgagca aactggaaat tcaatttgtt ttcgatgccc acaatgtaaa
      361 taccttctga ctgaaaatta attcttataa aggggaaaaa atgaaagttc catttcgatc
      421 taaaacaact tattgtgctg ggatcttagc atcgctttgc tttagccaat ctgttttagc
      481 tgatacgctc attactcact ggaatgaagc tgcacttcag gctattcgcg tgactcatcc
      541 aggtcctcct atcgtggcaa gagcactggc gataactcat acctgcatgt atgatgcttg
      601 gtctgcttat gatgccaagg cgaaaggcac gcgactggac gatgatttac gccgtcctga
      661 taatgagcat accatggcta ataaatctaa agcagtgagt tttgcagcat atcgttgtct
      721 atctgatctc ttcccatcgg aagtggccag ttttgatggg ataatgaaat cgcttggtta
      781 taatccagca gatcagtcga ctgataccaa taccgcagag ggaattggga atgtggctgc
      841 cgctgctgtt atccagtatc ggcatcaaga tggtgcaaat cagctcggag atttgaatgg
      901 cggcgctcct tatagtgatt atacaggcta tacgcctgtc aattcgcctt cttccattgt
      961 tgaccccaat cattggcaac cgttgcaggt tggttccaat atgcagagct ttattacccc
     1021 gcactggggc cttgtggaac cttatgcgct taaatcgggt gacgaatatc gtaagaaaat
     1081 tccagcccct gctaattatc ataccgagcc cgcacgctat cgtgtgcaag cacaacaagt
     1141 ggtggattat gcagcacatt taaccgatga aaagaaagtc attgctgaat attgggctga
     1201 tggtccgctt tctgaattgc cacctgggca ctggaccttg tttgcaacat ttgtctcaaa
     1261 acgtgatcgc cataaccttg atcaagatgt aaaaatgttt tttgcgctaa ccaatgccat
     1321 ttttgatgcc agcattgttt cttgggacgc aaagcggcat tttgattatg tacggcctgt
     1381 cacggcaatt cacttccttt tttctgggca acagattcca tcttggcaag ggttgatcga
     1441 tggtgcagac tggaaacctt atcaagctga tagtgttgtt acgccgccat tcccagagta
     1501 tttctcaggg cacagtattt tcagcgcagc tggcgcagaa acactcaaat tatttaccaa
     1561 aagcgacaat tttgggcata gcgtcgttgt gccggctaga tcatcccggg ttgaaccagg
     1621 tgtcgtccca gccagcgatc tcgttttgtt ctgggcgaca tttagtgatg cagccgatga
     1681 agcgggtatt tcgagacggt ttggtgggat acattttatc gatggcgatc tcgaagcacg
     1741 taaagtgggt cgtgtcgttg cccaaaatgc gtggaagaaa accttgaaat atttcggcga
     1801 taaagatcat aagcacgaca aagacgatga tcatggcaaa ggtaagaaat aatagcgtta
     1861 gtactagctt gctaagttaa gcttattgca tatacgtaac cgtaaccaac tgcctcatca
     1921 ttcatgatga ggcagttggt ttttaggaat ctcttcattt tacaaccatg agcgagcgac
     1981 tcacataagc gacaaaaatg attgcacaac aataggggcc aattaatccc caggcatagt
     2041 aattaccggg aatagcataa ttaagatagt aatctgctgc atcgaatgcc acgccgatca
     2101 atgcgaacag acccgaccaa aacgcgatcg atcttgcttt aatcgctccg cctattacac
     2161 caaatcctgc gaaggcacaa attaatcctg cactgccagc aagcgaagct gcaatcgaga
     2221 aatggccaaa aaacaattga cccatcaaaa atcctgtagg cgcgatcaat aagatataga
     2281 tacaagcaaa cggtaagagc gttttcatgg tggttgattt tatctgggaa ggcgtcagct
     2341 taaccgataa aataaaacaa cgatggctcg agaaaaaaca aaagataggt tattttatga
     2401 agtacaaaat actgattagt cttattcaga ggggtagtat tgattcgatc gtttcaataa
     2461 atgatcctcg agatggagtg aaaaagctca agcgattacc tttataatgc gggtaaatct
     2521 aacttatcat tcctcgccat ggaaaaaatc cgtttatcca aattgatgtc ggaacgaggc
     2581 atctgctcac gccgcgaagc tgatcgtttt atcgagcttg gttgggtatt tgtcgatggc
     2641 gagcgtatct ccgaacttgg catcaaaata tttccgacac agaaaattgc cttgaataaa
     2701 gcagcacaag cgcaacagac cgatttagtg acgattctgc tcaacaagcc gatcggctat
     2761 gtgtctgggc agccggaacc gggttatcaa ccagccgtga cgctgatcaa gccggaaaat
     2821 ctgttttcac ccaagcaagc aatgcaaccg gctaagcgat ttcagccgtg gcacttaaaa
     2881 aaactggccc ctgccggtcg ccttgatatc gattcgcaag gtttactggt attaacgcaa
     2941 gatggtcgca tcgccaagca aatgatcggt gagaattcac gcatcgaaaa ggaatatttg
     3001 gtgcgagttg aaggtgtgct gccggttgct aaactcgcat tgctgaatca tggtttgagt
     3061 ttggatgggc aggccctaaa accagcacag gtgagctggc aaaaccgcga tcaattgcgc
     3121 ttcattttga acgagggaaa aaaacgtcaa attcgccgta tgtgtgagct ggttggtctt
     3181 aaagtaaccg gtttgaaacg agtgcgcatt ggtaaagtga agctgggcaa tctacccgaa
     3241 ggtcaatggc gctatctgga aaatggtgaa aagttttaat ggtattttga ggtcattgca
     3301 tgcatcaacg cgtacgagtc gtgctgaaaa tcccagcgta tcaacttgag ctttattacc
     3361 aaggggttgt cgataccgtc gcggctgaaa ctactgacgg ccgcattgtg cattttccag
     3421 ccaatgtgct gcgctcggtg gtgcaaagtc atggtgtata cggtacgttt gaattggtat
     3481 tcgatgaaaa caataaattt gtatcgatca gtagcaccag ttgagggagt gctgaataat
     3541 tttgcgaaca taaagaagtg ggaagaacat aataagctgg ggaatagtat cagttagagg
     3601 ttaaaagtat taaagttgac ccatcaagta tgcgtgttaa actgattgta cttaattctg
     3661 taccagtaga ggtatttgtc atggatgcga ttagttatac aacagcacgt gctaatttag
     3721 ctaaaacaat ggaaagggtt tgcaacgatc attctcctgt tattattact cgtaagcgag
     3781 aagcgtcggt tgtcatgata tcgttagagg attatcaagc gatggaggaa accgtgtatt
     3841 tgctccgttc tcctgctaat gcacggcgat tgttggaatc tattgctgaa ctggaagctg
     3901 gaaaaggcac tgttcggcaa ttggctgaat gaacctgatt ttttctactc aggcttggga
     3961 agattatctt tactggcaaa aaactgataa atccacagta aaaagaattc atggtttaat
     4021 caaggcaatt cagcaaaaac cttttgaagg gattggcaaa ccagagccgc taaaacacgg
     4081 gttagctggt tattggtcaa ggcggattga taatgaacat agaatagttt ataaaatttt
     4141 gaatactgat ttgttcatcg cacaacttcg ttatcactat taaaggttct tttctgtgaa
     4201 cgacatccag caacttaccg agattttgct aaagtttcgc gatgagcgtg attgggcaca
     4261 gtttcataac gcgaaagatt tggcgctagc gctgaatatc gaggcagcag aattattgga
     4321 agtttttctg tggaaagcac cggaacaggc gaatatcgag agcgtaaaag aggagttggc
     4381 tgatgtgttg acatttgctt tgctgctgtc ggaaaaatat ggtttagacg tgaaacagat
     4441 tgtattagag aagctggaga aaaatgcctt gaaatatcca gtggataaat ctaaaggaag
     4501 cgctaaaaaa tatacggaat tgtaggtgtt gtggctattt tgcggcaagt tgccataagg
     4561 atgtcaattc tcttgtgcgg gctgtgtgca gtggatcact ggtatcggag acccgaggat
     4621 gatgaggttt gacgtcggat ttcgatatga tctttaggcc tgcagtatcg atcatgtgta
     4681 gtagttctgc acggctacgc aatcccaggt gttcggcaat atctgatagg atcagccaac
     4741 cttcacctcc tggaagaaga tgatcgcgca gaccttgtaa gaatcctcgc agcatgcgat
     4801 tttctgggtc gaatatggcg tgttccatgg gagaagcggg tcgagctggt atccacggtg
     4861 gattgcagac aatcaatgct gcctgcccct gaggaaaaag gtcggtttgc agtaattcga
     4921 gtttttcaat caaacccaaa cgtaacagat tgtcgcgtgc gcaatcgagc gcgcgtggat
     4981 catggtcggt tgcaacaatg cgttgaacac cccgttgcgc taatatcaca gccaatacgc
     5041 cggtgccagt accaatatcg aatgcgattg actgcatggt tagtatttta ggtagtgctg
     5101 cttgggcgat gagctggata tactcgttgc gaactgggga aaatacccca taatgcggat
     5161 gaatggtgat gcccagtacg gggatattga tacccttctt gcgccactcg tgtgcgccaa
     5221 ttaaacccaa cagttcgcgt agtgaagcaa catacggttc attacggggg ccgtatgctt
     5281 ccaagcaggc atggcgaata tctggtgcac ggggtaacgc gatagtatga tccgcttgaa
     5341 acgggatcag caatttatcg agtatttgcg cacgttgtga ttgcatcaga cgataacggt
     5401 gaaaagtatc ggcgactgac aattcggctg gtagatgctt cgatgtggat cttttcttgt
     5461 caatgcggcg agccatggct tggagcaact gcttggcgtt ttgaaaatcg ctgcgccata
     5521 gtaatgctgt gccttcacac gccaagcggt aagcattatc agcaggcata cgatcgtcaa
     5581 tgacctttac ctgttgcggt ggttgcaagc ttttttcact ttgccagcgg gcagattttt
     5641 cttctccggc ttcacgccag cggataatcg gattgttgct catgagggtg ctggtggtgt
     5701 tattggcagg gtggctgggc ataagagtgc tgaataatta gaaatagaaa tggcgctaga
     5761 ctacggattg taactgctaa tctagcgcca aaaccaaaga taagcgattt caactcacta
     5821 taaaactaat attggttggt cttattcagg cggaacaggg cgagggtgtc gatcggtatc
     5881 aatggcaaca taagtcagca cggcttcggt tactttgata caaatctctt caccaccctc
     5941 acgtaccccg cgttgtgcgt agaccgcgac atcaacggta atcgaggttc gtccaatttt
     6001 gataatatcc gcataaaaac tgactaaatc accgactaat accggttgct tgaaaacaaa
     6061 agagttaacc gcaatggttg caacccgacc tctagcccgt ctcaatgcag gaatgctacc
     6121 cgctatatca acttgggaca tgatccaacc cccaaagata tcccctgcat aattggtgtt
     6181 ggaaggcatt ggcaccatcc gcaaaaccgg ttggctttcc ggaagtggaa catgcaaaga
     6241 tagatcgctt gttactgagt tggttggcat ggtgtgatag gtgtcgttaa acctatgatt
     6301 caacgcttag tgtagagatt ctcgatttca gcttgatagc gttgtgcgat tttagaacgc
     6361 tttaatttta acgtgggtgt tagcatttca ttggcgacgc tccaaggctc tggaatcaaa
     6421 gcaactttga aaattttggc atagcctgga aattcatgga tttgctgcgc aatgcgatcg
     6481 agtagtattt tttctactga ctcctggtta atcaattggt gaggatcagg cggtaagtga
     6541 cacaactcgg caagggtttt caatcctcgc tgacttaata caactaacgc gccgagataa
     6601 gagcggcctt cgcctaccac catgacttgt tcgaagatgg gatcacgcaa gatggccgct
     6661 tccatatcgg caggcggaac tttctctccc gttgaggtta cgataatatc ttttaatcga
     6721 ccggtgatcg tcactcttcc ttgttcgtcg atttgcgcta tatctcctga gttaagccac
     6781 ccatcttccg agattactgc tttcgtcgct tctggattat tccaataccc aagcatgaca
     6841 ttcgggccgc gaatcaataa tgcattgttt tctcccagtt taacttcaac gcctgaaatt
     6901 gttcggccaa tgctggaagg tagattgtca tcgagtcggt tgacacaaac tacaggactg
     6961 ctttccgtca tgccatatcc ttgcagaatt gggagcccca agccaatgaa aatacgtgat
     7021 atctcaggcg ataatgcagc accaccactc atggcaaggc gtaatcgacc acctaattta
     7081 tccatgagtt tgcgcgcgac taatttttcg aggatcggcc agaatagatg ggagaaatgc
     7141 caagtatcac gaccttgctg ctgttcaaaa cggctgtaac ctactgatac agcaaaatag
     7201 aatagcttgc gtgctagcac agaaccctct gcgagtttgg tccgcaaacc ggcgtaaacc
     7261 cgttcgtaaa tccgtggcac cgaaacgagc acggttggct gaatcgtcag taaatcttct
     7321 tgcaataatt gaatggagcg agcgtaggca accgttgccc cgcacatcat cggtacataa
     7381 tagccagagg ttcgttcaaa cgtatgagag agaggtagaa acgaaagaaa tagatcatcg
     7441 ggtaagacat ctaccgcctg tgtcgaagca aaggtattag acaggatatt gtcgtggctt
     7501 agcataaccc ccttgggtcg cccagtggtt ccagaggtat agatgatcgt tgccagcgca
     7561 tcgggattgg tattgatatg cagcatttgt tccccttgct cgggtaacca ttgttgcagc
     7621 gtaacgacgc gcgggtcgta ccctcgtttc gttgcgtcat ccgtgactaa tagaattaca
     7681 atccgtaata ccgagctgag ttgagcaata attggaagaa aagcttccca ttgcgttgat
     7741 ttttcgacaa cgagcagttt tactcccgca ttctcaatgc aataggctgc attttctgca
     7801 cgatcttcgg tatacaaagg gacgacgacc aatccgagcc ccagtgctgc ttgctcgaaa
     7861 gctacccatt gcgggcagtt cctgcacata atggcaaccc gatcaccagg ggatagatct
     7921 tccctcgtca gcgcagcttg ccagcgtaca acccatcgtg ccatgtcagc ccaactgtag
     7981 tcgcgccatt gctcggttac actattgtag tcccgatagg ctatcttttg tggtgagcgc
     8041 gttacccgtt cactaaataa accatccagg gtttgggctg cttcaatggg gatgacatct
     8101 ttatgcatgt actgactttc gttcacgctt gatttccttg ttattgttgt tattagcagc
     8161 gatcaattaa tcaaaactcg gcgtgaaatc atcgaccata atgacccggc ggcgcaggat
     8221 tttcatttta tcgagtaaag cggcttcttc agcgctgatt aaattgagtg ccacggcttg
     8281 tgcgatcttg ccgtcccctt tagcagtcaa ttgaccctct ttcgttgcgg cacgtaatct
     8341 tgcttcaatc ggttcgcaat tgatcgcaca agataatgct tgttcaagtt gcgttagtgg
     8401 ttcattgtca tcggtgggaa cataaatacc cgctgttaag cgctcacgaa cttcacccgg
     8461 ttcgagaatg aggcgagcaa tgcgatgcga tagctcgtct gatggtggtg agcagcgttt
     8521 tccaagcggg aagacttgcc atctgagcca aatccgaata agaaaatggc caggaaaatt
     8581 gcttaagaat tcatcgaacg cttgctgggt tcgatatagt gcatcttgaa ttgaccagtg
     8641 taacaacggt
//
LOCUS       DAOQLJ010000099         8498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3324_length_8498_cov_6.176359, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000099 DAOQLJ010000000
VERSION     DAOQLJ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8498
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3324_length_8498_cov_6.176359"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="PK522_07800"
     CDS             <1..1151
                     /locus_tag="PK522_07800"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNC41673.1"
                     /translation="DRTMKTSTFKSMRLISYTLAGLFVSTSAQALFINATVTGGTPIL
                     GGTTDLLKSNRTVSDNNISTSVSASKTQATNATISQFDASQGVLVGVTTTLTAGNNTT
                     YLRADGANAARGSASVDTSWTGSGGLNTSGNLNSVSKSGNTDGADNSWNALSQTISNG
                     ANLNSWIGAGNLISTINTTINSNRIDGGSNIALIADISSSGANINGATAPSIIDLSAA
                     YSVTYNYMEHAVASFDGSSTLTSLDIDFGTIVLGDLVNSISFDIFNLAGVNGVALDLT
                     SISPSGDTATFVTDLGFFSDLIHGAHNSYSASFDPTSAGTFDATYLLTLSDDDATGAS
                     ASRHTYSLELHLTGTAVNPTAIPEPSMIALLGAGLLSLFGFSRRSSLK"
     gene            1539..1778
                     /gene="hfq"
                     /locus_tag="PK522_07805"
     CDS             1539..1778
                     /gene="hfq"
                     /locus_tag="PK522_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNC41674.1"
                     /translation="MSTKGQLLQDPFLNILRKEHIPVSIYLVNGIKLQGQIDSFDQYV
                     VLLKNSVTQMVYKHAISTVVPARAVSIPIESSIHE"
     gene            1778..2965
                     /gene="hflX"
                     /locus_tag="PK522_07810"
     CDS             1778..2965
                     /gene="hflX"
                     /locus_tag="PK522_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNC41675.1"
                     /translation="MHDSKILVVNQRSKALLVGFCQRDSDNESFEELRQLASSAQLDV
                     LEIVKSKRSRPNPATYIGTGKIDEITSLLKTSPEKISIVIFNNDLTPIQERNLTNRLE
                     CTVIDRTSLILDIFAQRAKSHEGKLQVELAQLEHLATKLVRGWTHLERQKGGIGLRGP
                     GETQLETDRRLLAKRVKFLKEKLSILQRQRDVQRLSRKRSQVKTISIVGYTNAGKSTL
                     FNRLTRSNSYAADQLFATLDTTTRKMFIPAYGNLVISDTVGFIRDLPHTLIAAFRATL
                     EETIQADLLLHVVDGTSPVKDDQIEAVNRLLEEINASTIPQILILNKIDQAGSKMRPG
                     YVRDEYGKITRILISAKTGEGLEYVKLALTEAIRYPSEEVDLEDVFLPEEKEVIDNSA
                     VAF"
     gene            2988..4172
                     /gene="hflK"
                     /locus_tag="PK522_07815"
     CDS             2988..4172
                     /gene="hflK"
                     /locus_tag="PK522_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNC41676.1"
                     /translation="MGLNDPQWGKRKGNAGPPDLDDVLRNFSKKINSLFGKRGGNNND
                     DEEPSGGSPRKPQGGNIFVIIMLLLVVWFASGFYIINEGQRGIVLRFGKHVDTTQAGL
                     RWHLPFPVEKVETVNVSQVRTVEIGYRNNVRSKVLKESLMLTDDENIIDIQFAVQYIL
                     NSPEKFLFNNRDPESAVLQVAETAIREAIGKSKMDYVLYEGREQVAARTTELMQMILD
                     RYEIGISVSRVTMQNAQPPEQVQAAFDDAVKAGQDRERQRNEGQAYANDVVPKARGTA
                     ARMLEEANGYQQRVISSAEGDVSRFQQVLVEYNKAPAVTRERLYLDMMQQVLSNTSKV
                     LVDQKGGNNLLYLPLDKLIQSDSALANRIPMVAQPQVIQEIPDTSGRTRESFRSRERE
                     VR"
     gene            4174..5052
                     /gene="hflC"
                     /locus_tag="PK522_07820"
     CDS             4174..5052
                     /gene="hflC"
                     /locus_tag="PK522_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNC41677.1"
                     /translation="MGKFSSALSGILTVLVIAISSSIYIVDEREQAILFQLGEVVDVK
                     TSPGLYFKIPIAQNVRYFDSRILTMDTEEPERFITSEKKNVLVDLFVKWRIVDVKQYY
                     ISVRGDEALAQTRLAQTVNSSMRDEFGNRTVHDVVSGERDKIMEIMRQKANVDAKKIG
                     VEVVDVRLKRVDLPQEVSDSVYRRMEAERKRVANELRSTGSAEAEKIRADADRQREVI
                     LAEAYREAQKIMGDGDGQAAAIYADTFQKSPEFYSFYRSVEAYKQAFKNKTDILVLEP
                     SSDFFKYIKGAAEGKK"
     gene            5144..5329
                     /locus_tag="PK522_07825"
     CDS             5144..5329
                     /locus_tag="PK522_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HNC41678.1"
                     /translation="MWEVFFIAIGLMMIFEGLFPFSFPNAWRETFQKLILLEDNQIRF
                     IGLTSIVVGLIILLLVN"
     gene            5378..6547
                     /locus_tag="PK522_07830"
     CDS             5378..6547
                     /locus_tag="PK522_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNC41679.1"
                     /translation="MRNWILPEYVEDILPAESLHIEKIRRQMLDLMHVHGYQLVIPPL
                     LEYTESLLSGSGSDMDLHMFKVVDQLSGRMLGLRADITPQAARIDAHLLNAQGVTRLC
                     YASSVLHTIPCEIARTREPFQIGAELYGHSGLESDIEIQHLMLQCLVKSGVGKIQLDL
                     GHVAIFRCLIKDLAIKPALETDFYTLLRAKDLAALKELLQSTSTLLSKNAQEMLLHLP
                     ELYGDRKILIEARKLLPEHPEIIAALDQLEVLAEKLACYVDRITFDLSDLRGYHYHTG
                     IVFAAYTKGNPGPIALGGRYDEIGKSFGRSRPATGFSMDLRELSRLAHTETSPKGILA
                     PYKPDDKLLQEKVIQLRASGWVVITLLPGCNEENDATCCDKKLVLKNDTWDVVEI"
     gene            6586..7908
                     /locus_tag="PK522_07835"
     CDS             6586..7908
                     /locus_tag="PK522_07835"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNC41680.1"
                     /translation="MAKNVVVIGTQWGDEGKGKIVDWLTDHAAGVVRFQGGHNAGHTL
                     VIDNKKTVLHLIPSGILRSSVMCYIGNGVVLSPRALLEEIDILESAGISVSPRLRISE
                     ACPLILPYHAALDSARETAKGSAKIGTTGRGIGPAYEDKVARRAIRLQDLLCKERFEK
                     KLEEILDYHNFVLKHYFHTTTIDLKEVLDEGLAQAERIRPMVADIPELLFQAHKRGAS
                     LLFEGAQGALLDIDHGTYPYVTSSNCIAGAASTGSGVGPQMLHYVLGITKAYTTRVGS
                     GPFPTELDDETGSHLAKRGHEFGSTTGRPRRCGWFDAVAMRRSIQINGVSGLCITKLD
                     VLDGLEKLKVCIGYKNNSTANSVTSSTLNTLPVGAEDLVACEPVYKELPGWSENTKGI
                     TDFKALPSAAQSYLRCLEEVCEVPLDLISTGPDRAETIVFRHPFVDAT"
     gene            complement(7972..8418)
                     /locus_tag="PK522_07840"
     CDS             complement(7972..8418)
                     /locus_tag="PK522_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41681.1"
                     /translation="MMKRMLWLKVIGLFVLGFYFSTISAQQSGQLIIEAQIQDLEASL
                     LRTTQHQQSVFQQFQMLQEIRRFEVAQDSFTTLSQSPQIGGTLNSPIVDYDDAVRAKQ
                     DRQRKLQQYTLDLETLYQRFQELESEKRMIMEQIDLLNRQKKQKVE"
BASE COUNT         2568 a         1605 c         1860 g         2465 t
ORIGIN      
        1 aggacagaac tatgaagacc agtacattta aatcaatgcg tttaataagt tatacattgg
       61 ctggtctttt cgttagtaca tcagcacaag cattatttat caatgcaacg gtaactggtg
      121 gaacccctat tctgggtgga acgactgatc ttcttaaaag taatcgcaca gtttctgata
      181 ataatatctc aactagcgtt tcagcatcca aaacgcaagc aacgaacgca acgattagcc
      241 aattcgatgc ttctcaaggt gttttagttg gggtaacgac tacacttaca gcaggcaata
      301 atacaaccta ccttcgtgct gatggtgcga atgctgcgcg aggctcagcg tcagtagaca
      361 ccagttggac aggaagcggg gggttaaata cctcaggtaa tcttaattct gttagcaaaa
      421 gcggaaatac cgatggtgca gataacagtt ggaatgcatt atcacaaact atcagtaatg
      481 gggcaaacct aaatagctgg ataggtgctg gaaatttaat ctcaaccatc aatacaacga
      541 taaattctaa tcgaatcgat gggggtagta acattgcgct tatcgctgat attagtagtt
      601 ctggggcaaa tattaatggc gctacagccc cttcaattat agaccttagc gcagcttatt
      661 ctgttaccta taattacatg gaacatgcgg ttgcatcgtt tgatggtagt tcgactttaa
      721 cctcgttgga tattgatttt ggaaccattg tactgggtga tctagtcaat tcgattagct
      781 ttgatatttt taatctggcc ggagtcaatg gtgttgcgtt ggatttgaca agcatttctc
      841 caagtggaga taccgctact tttgtgactg acctcggatt tttctcagat ctcattcatg
      901 gcgctcataa tagctacagt gcttcatttg atcctaccag tgcaggtact tttgatgcaa
      961 cgtatttact gaccttatca gatgatgatg caacgggggc ttcagcaagc cgccatactt
     1021 atagcttaga actccattta acgggtactg cagttaatcc aacggcgatt cccgaaccca
     1081 gcatgatcgc gttacttgga gctggcttgt taagcctgtt cggtttttca agacgctcta
     1141 gtttaaaata ataaatcatt tcaatcattc agaaagaaaa agcgctggta gactatctat
     1201 caacgactac cagcgctaag aagtagtccg caattattct tttctcctcc gtaaattttg
     1261 cttttgttcc actgctaact tccaattact gacgttggta cgattttcag cgataatccc
     1321 tcttctttct acattgcctt tacttacaac taaacttagc tgagtttaat cgaagagtcc
     1381 cacttacaaa tcgatattaa gtaatttggg cctattgcct tttaaataaa ttaggtttgt
     1441 ttgattgctt cagaaatgag caaggtatag atttggatag aaaaataaag tagagtctga
     1501 aaaataatta agaaacgatt atataagggg gtttattaat gagcacaaaa gggcaattac
     1561 tgcaggatcc ttttttgaat attctacgaa aagaacatat tcccgtatcg atctatttag
     1621 taaatggcat caagcttcaa gggcaaattg attcttttga tcaatacgtt gtgctactga
     1681 agaattcggt aacgcaaatg gtttacaaac atgccatatc aactgtggtt cccgcaagag
     1741 ctgtatcgat tccaatcgaa tcttcaattc atgaataatg catgattcaa aaatactagt
     1801 ggttaatcag cggtcgaagg cacttttggt cggattctgc cagcgcgata gtgacaatga
     1861 aagcttcgag gagttacgac aacttgcgag cagtgctcaa ctcgatgttt tggaaatcgt
     1921 gaagagtaag cgatctagac caaatccagc aacttatatc ggaacaggaa aaattgatga
     1981 aatcacttcc ttgctcaaaa ctagtccgga aaaaatttcg atcgttatct ttaacaatga
     2041 cctcacaccc attcaagaac gaaatctgac taatcgactc gaatgcaccg ttattgatcg
     2101 aacgagtttg attctggata tttttgcaca acgcgctaaa agtcatgaag gtaaattaca
     2161 agttgagctg gcgcaattgg aacaccttgc cactaaatta gtaaggggtt ggactcatct
     2221 tgagcgacaa aaagggggga tcggacttcg aggtcctggg gaaactcagc ttgaaacaga
     2281 ccggcgacta ctggcaaaac gagttaaatt tcttaaggaa aaattatcaa ttctgcagcg
     2341 acagcgcgat gttcagcggc tatcacggaa acgatcgcaa gtaaaaacaa tttcgattgt
     2401 tggttataca aatgcgggaa aatctacatt atttaatcga ttaactcggt caaacagtta
     2461 tgccgcagat caattgtttg cgacgctcga tacgacaaca cgtaaaatgt ttatacccgc
     2521 ttatggaaat ttagtcattt ctgatacggt tggttttatt cgagatttgc cgcatacctt
     2581 gattgcagca ttccgggcta ctctagaaga aactattcaa gcagatttat tgttacatgt
     2641 tgtagatgga accagtccag taaaagacga tcaaattgaa gcagtgaata ggcttcttga
     2701 agaaataaat gcttcaacta tcccacagat tctaatactc aataaaattg atcaggccgg
     2761 ttcaaaaatg agacccggct atgttcgtga tgagtatggt aaaatcactc gtattctcat
     2821 tagtgctaaa acgggtgagg gacttgaata tgtcaagcta gccttaactg aggcaatcag
     2881 gtatccttca gaagaggtgg atctagaaga tgtttttttg cctgaagaaa aagaagtaat
     2941 agataacagc gcagtggctt tttgaacgag agaaaggatt aattattatg ggcttaaatg
     3001 atccacaatg ggggaaaaga aagggtaatg caggtccacc cgatttggac gatgttttac
     3061 gaaatttctc aaagaaaatt aatagtcttt ttggtaaaag aggcggaaat aataacgatg
     3121 atgaagaacc aagtggtggt agtcctagaa aaccacaagg tggaaatatt tttgtcatca
     3181 ttatgcttct gctcgttgta tggtttgcca gcggctttta tatcattaac gaggggcaac
     3241 gcggtattgt gcttcgtttc ggtaaacacg tagatactac ccaggcaggt ttgcgttggc
     3301 atcttccttt tccagttgag aaagttgaaa ctgttaacgt aagtcaagtt cgcacagtag
     3361 agattggata tcggaataat gttagaagta aagttctgaa agaatccctc atgttgacgg
     3421 acgatgaaaa tatcatcgat attcagtttg cggttcaata tattctgaat agtcctgaaa
     3481 aattcttatt taataatcgt gatccagaaa gcgctgtatt acaggttgct gaaacagcga
     3541 ttcgtgaggc aattggtaaa agtaagatgg attatgtttt atatgaaggc agggagcaag
     3601 tggctgccag aacaactgaa ctcatgcaaa tgattttaga tcgatatgaa atcggtattt
     3661 cggtaagccg tgtgacgatg cagaatgcgc aacctcccga acaagttcaa gctgcttttg
     3721 atgatgcggt taaagcggga caagatcgag aacgtcaaag gaacgaaggt caagcctatg
     3781 cgaatgatgt agttccgaaa gcaagaggta ctgcagcgag aatgctagaa gaagccaatg
     3841 gttatcagca gcgtgttatc tcaagcgcag agggtgatgt cagtcgcttc cagcaagtgt
     3901 tagttgaata taacaaggca cctgctgtta cccgtgaacg gttatattta gatatgatgc
     3961 aacaggtttt atcaaatact agtaaagtat tagttgacca gaaaggggga aacaatttgc
     4021 tgtatttacc attagataaa ttaattcaat ccgattcagc attagcaaat cggattccaa
     4081 tggtggcaca acctcaggtc atccaagaaa ttcctgatac cagtggacgt acacgcgagt
     4141 cttttcgcag cagagaacga gaggtgagat aacatgggaa aattttcttc agcattatca
     4201 ggtattctca cggtacttgt catagcaatt agctcttcaa tctatattgt cgatgaaaga
     4261 gaacaggcta tcctatttca attaggggaa gtcgtcgatg ttaaaacttc acctggccta
     4321 tatttcaaga ttccgattgc tcagaatgta cgatatttcg attcgcggat tctgacaatg
     4381 gataccgagg aaccagaacg attcattact tcagaaaaaa agaacgtttt agttgatttg
     4441 ttcgtgaaat ggcgtattgt cgatgtaaaa caatactata ttagtgtaag aggcgatgaa
     4501 gcacttgcac aaacgcgcct tgcgcaaact gtcaactcaa gcatgcgtga tgaattcggt
     4561 aaccgtactg ttcatgatgt agtgtccggt gagcgggata aaattatgga aatcatgcgc
     4621 cagaaagcga atgttgatgc caagaaaatt ggtgtagaag tagtcgatgt tcgtttaaaa
     4681 cgtgtagatt taccacaaga agttagcgat tcagtttatc gtagaatgga agctgaaagg
     4741 aaacgcgtag ccaatgagtt acgttcgacc ggttctgctg aagctgaaaa aattcgcgca
     4801 gacgcagata gacaacgaga agttattctg gcggaagcct atcgagaagc ccaaaagatt
     4861 atgggggatg gtgatggtca agctgcagca atttatgcag acaccttcca gaagagtcct
     4921 gagttttatt ccttctatcg cagtgtagaa gcttacaaac aagcatttaa gaataaaact
     4981 gatattctcg tgcttgaacc aagttctgat ttcttcaaat acatcaaagg cgctgcagaa
     5041 gggaagaaat aattactaat tcactaaaat actatcgatc aatgcgaatg ttattaaaga
     5101 gaaagccatt gatcgatagt caaagattgg agttatttgc ttaatgtggg aagtcttttt
     5161 cattgcaatt ggattgatga tgatatttga aggattgttt cccttctcat ttcccaatgc
     5221 atggcgcgaa acattccaga agttgatcct gttagaagat aatcaaatac gatttattgg
     5281 cctaacgtct atcgttgtag gtttaatcat tcttctttta gtgaattaga tcagacgcta
     5341 tttcttcaac tgtaacttac cttggctttc cctttcgatg cgtaattgga ttcttcctga
     5401 atatgtcgaa gatattcttc ctgccgagtc attacatata gaaaaaatcc gccggcagat
     5461 gctggattta atgcatgtgc atggctacca actcgtcatt cctcctttat tagaatatac
     5521 cgagtcctta ttgtcgggta gtggtagcga tatggatcta cacatgttca aagttgttga
     5581 tcaactcagt ggaagaatgt tgggactccg ggcagatatc acaccacagg cagcacgcat
     5641 tgatgcacat cttttgaacg cccaaggtgt tactcggctt tgctatgcca gcagtgtcct
     5701 acataccata ccctgcgaaa ttgctcggac acgtgaacct tttcaaatag gtgcagagtt
     5761 atacggacat tcaggtttag aaagcgatat cgagattcaa catttaatgt tgcaatgtct
     5821 tgtaaagtca ggggtcggaa aaatacaatt agacctggga catgttgcta ttttcaggtg
     5881 cttaattaaa gacttagcaa ttaaacctgc actggaaaca gatttctata ccctgctacg
     5941 cgctaaggat ttagcagcac ttaaggaatt acttcaatcg actagcacct tactcagtaa
     6001 aaacgctcag gaaatgttat tgcatttacc tgaattatat ggcgacagaa aaatactcat
     6061 tgaagcacgt aaactgctac cagagcaccc tgaaatcatc gctgcacttg accagctcga
     6121 agtgcttgca gaaaaattag catgttacgt ggatcggatt acgtttgatc tatctgattt
     6181 aaggggctac cactatcata ctggtattgt ttttgctgcc tatacgaaag gaaatcctgg
     6241 gcctattgcg ttgggtggca gatatgatga gattggtaaa tcttttggaa gatcaaggcc
     6301 agcaacaggt tttagtatgg atttacgtga attatctcgg ctagcacata ctgaaacgag
     6361 ccctaaagga attttagcgc cctataagcc tgatgataag ttactacaag aaaaggtgat
     6421 acaactgcgg gcatcgggct gggttgtcat cacacttttg ccgggttgta atgaagaaaa
     6481 tgatgccact tgttgtgata agaagctcgt tcttaaaaat gacacttggg atgttgtaga
     6541 aatttgatac tgccttaagt aattcaggtg aagagaaata aagctatggc taaaaatgta
     6601 gtggtaattg gtacccaatg gggtgatgaa ggaaaaggga aaattgttga ttggttaacc
     6661 gatcacgcag cgggtgttgt tcgttttcaa ggtggacata atgctggtca taccttagtc
     6721 atcgataata aaaaaactgt attacatctt atcccatctg gaatattgcg tagttcggtc
     6781 atgtgctaca ttggtaacgg agttgttctg tcaccacgcg ccttattaga agaaatcgat
     6841 atccttgaaa gtgcaggtat ctcagtatca cctcgattaa gaattagtga agcttgtcca
     6901 ttaatcttac cgtaccatgc cgccctagac agcgcacgtg agaccgcgaa aggatcagct
     6961 aaaattggca caaccggacg tggcattggc cccgcctatg aggataaagt ggcgcgacgg
     7021 gctatccgtc tgcaagactt actttgtaaa gaacgatttg aaaagaaact cgaagaaatt
     7081 cttgattacc ataatttcgt tctcaaacat tactttcata ccacgacgat agatttgaaa
     7141 gaagtgcttg atgaaggact tgctcaagca gaacgaattc gaccgatggt agccgatata
     7201 cccgagttac tgttccaggc ccataagcgc ggagctagtt tactatttga aggtgctcaa
     7261 ggagcattac tcgatattga ccatggcact tatccctatg ttacctcaag caactgtatt
     7321 gcaggcgctg cttctactgg gagcggtgtt ggtccacaaa tgttacatta tgtactgggg
     7381 ataaccaaag cttatacgac tcgcgtgggt tctggcccct ttcctaccga acttgacgat
     7441 gaaacaggtt cacatttagc aaaacgggga catgagtttg gatctaccac agggcgtcct
     7501 cgtcgctgtg gatggtttga tgctgtcgca atgcggcgat cgatacaaat caatggggta
     7561 tccgggttat gtattactaa attagatgtt ttagatggtt tagaaaaatt aaaagtttgt
     7621 attgggtata aaaataattc tacagctaat tcagtcacaa gtagcacttt gaatactttg
     7681 ccagtgggtg ctgaagatct tgtggcctgc gagccggttt ataaagaact acccggatgg
     7741 agtgaaaata ctaaaggtat tactgatttt aaagcgttac caagcgcagc tcaaagttac
     7801 ttacgttgct tggaagaagt atgtgaagtt ccattagatc ttatttctac tgggcctgat
     7861 cgcgccgaaa caatcgtatt tagacaccct tttgtagatg caacctaaga taacttgcct
     7921 agaaaatctt actttgctgt atgtactttc aacaatacca atcactcccc ctcattctac
     7981 tttttgcttt ttctggcgat tcagcaaatc aatctgttcc ataatcatcc ttttctcact
     8041 ttctagttct tggaatcgtt gataaagcgt ctcgagatca agcgtatatt gctgtaattt
     8101 cctctgtcga tcttgtttag cgcgaaccgc atcatcataa tcaacgatcg gtgagttcag
     8161 tgttccccca atttggggcg attgggataa tgtggtaaag ctatcttgcg ctacttcaaa
     8221 ccggcgaatc tcttgcaaca tctgaaattg ctgaaaaaca ctttgttgat gttgagtcgt
     8281 tcgtaataag ctcgcctcta aatcttgaat ttgtgcttca atgatcagct gacctgattg
     8341 ttgcgctgag attgttgaaa aataaaaacc aagaacgaac aatccaatca ctttgagcca
     8401 taacatacgc ttcatcataa atttttttgc actgaatgat cgatgatagt actggtttta
     8461 taatttgcgg tggggacatt cagctttgtt gcaatctg
//
LOCUS       DAOQLJ010000100         8487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3329_length_8487_cov_6.186907, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000100 DAOQLJ010000000
VERSION     DAOQLJ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8487
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3329_length_8487_cov_6.186907"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PK522_07845"
     CDS             <1..517
                     /locus_tag="PK522_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNC41682.1"
                     /translation="PLQLTFATMILANNGHAFRPHFVKQIHDSKTGRIYENMIKQLYP
                     LNLKNENLMRVRNALVDVTRPGGTAARAGANASYTFAGKTGTSQVINIKQGERYVASK
                     INERHRDHAMFIAYAPAEDPKIVVTVLVENGGSGGSTAAPIARKVFDYYLLGQMPEPL
                     QSDSVNNSRRD"
     gene            586..1689
                     /gene="rodA"
                     /locus_tag="PK522_07850"
     CDS             586..1689
                     /gene="rodA"
                     /locus_tag="PK522_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNC41683.1"
                     /translation="MDSIDRIWQYCTRYIDGFLLGAIFLLMITGLIVLYSASGGNFGR
                     VNSQFVNMLIAVFIMWVIANIPLQQLMRLALPLYFIGIILLIAVALFGEVQNGARRWL
                     NIGVTRIQPSELLKIAVPLMMAWYFEKTEIKLRLRDYAGGALILILPVLLIARQPDLG
                     TAILISASGFYVLYFAGLSWRVMGSLAIIAAAAIPLFWSFGMHDYQRKRVLTMLDPSQ
                     DALGAGYHIIQSNIAIGSGGITGKGWQNGTQSQLDFLPEQSTDFIFAVFSEEFGLMGN
                     SLLLLLYLIIIGRCIYITANAPTQFTRLVAGSITLTFFTYVFVNMGMVSGILPVVGVP
                     LPLISYGGTSLVTMLLGFGILMSIQTHPKLVKT"
     gene            1824..2606
                     /locus_tag="PK522_07855"
     CDS             1824..2606
                     /locus_tag="PK522_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNC41684.1"
                     /translation="MAPVTSPTESQNKNYYLDDGPEDDPPTNLDTIPSANPQPEAIRP
                     ANMRPYMVFGQVYTPMTSIEPYKQKGIASWYGRRFHGKPTASGEVYNMYGMTAAHPTL
                     PIPSYARVTNLNNGKSIVVRINDRGPFIANRLIDLSYTAAYQLNILTNGSGPVEVESI
                     IPQSDQFASNRANQAPKISPINPTNQIFLQLAAFGSKHNADKYLLQIRQELATIGENI
                     NITQEGNLYKVHIGPYANHASAKEIAHEILQRTTIKPLLTMR"
     gene            complement(2629..4974)
                     /locus_tag="PK522_07860"
     CDS             complement(2629..4974)
                     /locus_tag="PK522_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNC41685.1"
                     /translation="MAGVCWLQQQATLPESSAIWILPLLVVLIAGFANYSTRLARFIN
                     AFLIFCFFLTLGFLWAGVNAHWRLADELPEEWESRDIAVTGVIAKLPEITRQGIRFQF
                     DIEKIQTEGAYVPKHILITWYQNQHRAIKIQPGERWQLTVRLKRPHGNANPYVLDTEI
                     KYLERNIRAIGYVRYSDQNIRLDEEVRTPFYFIERLRDQIRKRFNEVLSDHPYSGVLI
                     ALAIGDQRAIPKDQWTLFIQTGTSHLMAISGLHVTMISGIAFTFFYWLWCRFPQLLVR
                     LPARKFALSAGLVIALGYVLLSGFAIPARRAFLMLCVIVIGLWQDRRIAVSTLLKWAL
                     IIVVMFDPWAVTSPGFWLSFGAMAWIAYIAIDRIGSLQTNKISNWITIQWVITIGLVP
                     LLLILFQQFSLISPVANALMIPLVSLAVVPLTVVSVMPAFDFLLIPAHLALTGGMTLL
                     QGLSELTDTTWQQHQPPLWAIIAGMLGIFWFFLPGGRGLFLTSGFPSRWLGIMGLLPL
                     FLISPPKPSQGELWVTVLDVGQGLSIVVQTENHVLLFDTGPRYGDSNSGERIIIPFLR
                     GAGIQHIDQLVISHADSDHSGGLSSILGEVPVKTIVSSVELDHPVLSAVPQSQPCRAG
                     DAWIWDGVLFEFLYPLSPERKQRVQRSRQNNSSCVLKITTAGDSILIPADIEKEAELD
                     LLKYFSHLLPSTVLIAPHHGSKTSSQAAFIQSVNPQLTIFTVGYRNHLKHPNQAVVER
                     YQAIGSQMMRSDYQGAITLQFSQHGFTAKSWRETKPRYWYQ"
     gene            5171..5857
                     /gene="rpe"
                     /locus_tag="PK522_07865"
     CDS             5171..5857
                     /gene="rpe"
                     /locus_tag="PK522_07865"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNC41686.1"
                     /translation="MFRIAPSILSANFAKLGEEITNVLDSGADIIHFDVMDNHYVPNL
                     TIGPLVCEAIRPLTKAMIDVHLMVEPVDRIVPDFAKAGADIISFHPEASNHIDRTIGL
                     IKEQGCKAGLVFNPATPLSYLDHILDKLDLILIMSVNPGFGGQKFIPEALNKLRAVRQ
                     RIEASGKKDILLEIDGGVKVDNIAEIARAGADTFVAGSAIYGAGKDSDPHRYDTVLAA
                     MRSELAKVKA"
     gene            5918..6643
                     /locus_tag="PK522_07870"
     CDS             5918..6643
                     /locus_tag="PK522_07870"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HNC41687.1"
                     /translation="MTHSASSQQIYLKKEIANPLAIKAVVIDLDGTLLDTAMDLATAA
                     NLMLVELGKPELSFATIQTFIGKGIQKLVKRALTNHLDVEPDPALFEKALPIYEHHYR
                     ENLSKQTRPYPGVVEGLKILQQKGFRLACITNKAEAFTVPLLKATHLFDYFELVLSGD
                     SLPRKKPDPLPLLHACQFFQIEPHQLLLIGDSLNDAIAARAAGCAIFCVPYGYNEGQD
                     VKELDCDAIVPTIAAATEIIENA"
     gene            6700..8178
                     /gene="trpE"
                     /locus_tag="PK522_07875"
     CDS             6700..8178
                     /gene="trpE"
                     /locus_tag="PK522_07875"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNC41688.1"
                     /translation="MNEDKFNELAAQGYNYIPFVLETFADLDTPLSIYLKLANQPFSY
                     LLESVLGGERFGRYSVVGLPARTRLEVFKQQINIIKGVATETHHVTDVLGFIESYLEN
                     YKIAPNDDFDLPRFYGGLVGYFGYDTIRYIEPKLSNCSKPDTLNTPDILLLLSEELVV
                     IDNLAGKIYLIVFINPNTDHAYQTGKDRIKALLKQLRQPVTIPPESPHSPSTATSEFS
                     ESAFIAAVERAKQYIIDGDIMQVVLSQRTSLPYTASPLALYRALRSLNPSPYMFYYHL
                     GDFHIVGASPEILVRLEKNVVTVRPIAGTRPRGKHFQEDQALATELLADPKERAEHVM
                     LMDLGRNDISRVAQTGTVKVTENMTIERYSHVMHIVSNVEGQLEPNLTAIDVLRATFP
                     AGTVSGAPKIRAMEIIDELEISKRGIYAGAVGYLGFNGDMDLAIAIRTGVIKDGKLYV
                     QAGAGIVADSIPYNEWVETQNKAKAVLRAAEIAEKGLDSKIN"
     gene            8208..8299
                     /locus_tag="PK522_07880"
     tRNA            8208..8299
                     /locus_tag="PK522_07880"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8242..8244,aa:Ser,seq:gga)
BASE COUNT         2473 a         1950 c         1730 g         2334 t
ORIGIN      
        1 cccgctacaa ttaacgtttg ccactatgat ccttgcaaat aatggccatg ctttccgtcc
       61 ccattttgta aagcaaatac acgacagcaa aacggggcga atctatgaga atatgattaa
      121 acaactttat cccctcaatc tcaaaaatga aaatttgatg cgagtgcgga atgctttggt
      181 tgacgtaaca cgccccggag gtactgcggc aagagcgggc gccaatgcca gttatacctt
      241 tgctggaaaa acaggcactt ctcaagtcat caatatcaaa caaggtgagc gttatgtagc
      301 aagcaaaata aacgaacgcc atcgcgatca tgcgatgttt attgcttatg cgccggctga
      361 ggaccctaaa attgtagtca cagttctcgt tgaaaatggt ggttctgggg gatcgactgc
      421 cgcacctatt gcgcggaagg tttttgatta ttatctatta ggacaaatgc cagagccatt
      481 acaatcggat agcgtcaata atagccgccg tgactaattt ctggttaatc gtattcacga
      541 tttacgatcg ttccaataac ccattaacct attgacttct ctcgaatgga tagcattgat
      601 cgaatctggc aatattgcac acgctatata gatggcttct tactcggtgc catttttctg
      661 ctgatgataa ccgggctgat cgtactctac agcgcaagtg gcggtaattt cggtcgagta
      721 aatagtcagt tcgtgaatat gctgatcgca gtttttatta tgtgggttat cgcaaatatc
      781 ccgctccaac agctcatgcg attagcgcta ccgttgtatt ttatcggtat catcttattg
      841 attgctgtgg cactttttgg tgaggttcaa aatggtgcgc gacgttggtt aaatattggc
      901 gttacccgta tccaaccttc tgaacttcta aaaattgcgg tgccactgat gatggcttgg
      961 tattttgaaa aaacagaaat aaagttacgt cttcgggatt atgcaggcgg tgcgcttatt
     1021 ttaatattac cagtactact cattgcgcgc caacctgatc ttggtacggc aatattaatt
     1081 agtgcgagtg ggttttatgt actctatttt gcgggattat cttggcgagt aatgggcagt
     1141 cttgcgatca tagctgctgc agcaattcca ttattttggt cattcgggat gcacgattat
     1201 cagcgtaaac gggttctaac gatgctagac ccatcccaag acgcacttgg cgcgggttat
     1261 catattattc aatcgaatat cgccattggg tcgggtggta ttaccgggaa aggatggcag
     1321 aatggtacac aatctcaact cgatttttta ccggagcaaa gtacagattt catttttgct
     1381 gtattctcag aagaatttgg attgatgggc aatagcttat tactgttact ttatctcatc
     1441 attatcggac gttgcatcta tataactgcc aatgcgccca cgcaatttac gcgccttgtt
     1501 gccggttcga ttacgctgac tttttttaca tacgtatttg ttaatatggg catggtcagt
     1561 ggtattttac cggttgtggg tgttccactt ccattaatta gctatggtgg tacatcattg
     1621 gtcacgatgc tcctgggttt tggtatcctc atgagcatcc aaactcaccc taagctagtc
     1681 aagacatgag gtaccttcaa atgcatttta agaaagattt ttttaatagc agcctaaact
     1741 ggcttagttt cttcttagtt atcaccctac tttatgggtg tagcagccct gctccctttg
     1801 ttcgttctgg cgaaaaaatt tcgatggccc ccgttacatc accaacagaa tctcaaaaca
     1861 aaaattatta tctcgatgat ggccctgaag atgatccccc cacgaatcta gacaccatcc
     1921 ctagcgcaaa tccccaacca gaagcaatta gacctgctaa catgcgacct tatatggttt
     1981 ttggacaagt ctatacaccc atgacgagta tcgagcctta caaacaaaaa ggcattgcat
     2041 cttggtatgg ccgtcgtttt cacggtaaac cgactgcttc gggtgaagtt tataatatgt
     2101 atggtatgac agctgctcac ccgactttgc caatcccaag ctacgctcgc gtgacaaacc
     2161 taaataatgg aaagtccatc gttgttcgaa tcaatgaccg ggggcctttc attgcaaacc
     2221 gactcataga cctatcttat acagcagcct accaacttaa tatcctgacg aatggcagtg
     2281 gaccagtcga ggtagaaagc atcattccgc agagcgatca attcgcttca aatcgtgcaa
     2341 atcaagctcc taaaatcagt cctatcaatc ctaccaatca aatttttcta caattggcag
     2401 cgtttggttc aaaacataat gcagataaat atctgctaca aattcgccag gaactcgcaa
     2461 ccattggtga aaatattaac atcacgcaag aaggcaatct atataaggtc catattggcc
     2521 cctatgctaa tcatgcatca gccaaagaaa tcgctcatga gattctacaa cgcacgacaa
     2581 ttaaaccttt gcttaccatg cgttaatgat tcgatgaccc gtaccacgtc actgatacca
     2641 gtaacgtggt ttcgtttccc gccaactctt ggcagtaaaa ccgtgttgtg agaattgcag
     2701 tgtaatcgcc ccttgataat cactgcgcat catctgactt ccaattgcct gatagcgttc
     2761 aaccactgct tgattagggt gctttagatg attgcgataa cccaccgtaa agatagttaa
     2821 ttgcggattg acgctttgaa tgaaagcagc ttgagaagaa gttttacttc catgatgcgg
     2881 tgcaatcaaa acggttgatg gcaataaatg agaaaaatat tttaataaat ctaactcagc
     2941 ttctttttca atatctgctg gaatgagtat actgtccccg gcagtcgtta ttttcaatac
     3001 gcagctcgaa ttattctgcc tgcttcgctg taccctttgt ttgcgttcag gcgataaggg
     3061 atacaaaaat tcaaataaaa caccatccca tatccaagca tcacccgctc gacaaggttg
     3121 actttgtgga accgcagata aaactggatg gtccaactct actgatgaca caattgtttt
     3181 gacaggcacc tctcctaata tcgatgataa tcctccacta tgatctgaat ctgcatgaga
     3241 tatcactaac tgatctatgt gttgaattcc tgcgccccgt aaaaagggaa taatgatccg
     3301 ttcgccgcta ttgctatccc cataacgcgg cccagtatca aacagtagca catgattttc
     3361 tgtttgaacc acaattgata acccctgccc gacatctaat accgttaccc ataattcgcc
     3421 ttggcttggt ttcggtggtg aaatgagaaa caaaggtagt aaacccatta tgcctaacca
     3481 tcgcgaagga aagcccgaag ttaagaacag tcctcttcca cccggtagaa aaaaccagaa
     3541 aattcctagc attccagcta tgattgccca taaagggggc tgatgctgtt gccaagtggt
     3601 atctgttaac tcactgagtc cttgcagtaa tgtcatcccc cctgttaatg cgaggtgagc
     3661 aggtatcagt aaaaaatcga atgctggcat aactgaaact accgtgagtg gcacaaccgc
     3721 gagactgacg agaggaatca ttagcgcatt ggcaactgga gatatcagcg aaaattgctg
     3781 gaacaaaatc agcaacaatg gaacaagccc aatggtgata acccactgaa ttgtaatcca
     3841 gttactgatt ttattggttt gtaaactacc aattcgatcg atcgcaatat aggcaatcca
     3901 agccattgca ccaaacgata accaaaaccc cggtgatgta acagcccaag gatcgaacat
     3961 cactacgatg atcaacgccc atttaagtag cgtagaaact gcaatgcggc gatcctgcca
     4021 taatccaatt acaatcacgc ataacattaa gaatgcacgg cgcgctggta tagcgaaccc
     4081 tgagagcaat acatacccca atgcaatgac caatcctgcg cttaatgcaa atttacgagc
     4141 aggtaatcgt actaagagct gcgggaagcg acaccaaagc caatagaaaa aagtaaaggc
     4201 aatccctgaa atcatcgtca catgaagtcc tgaaattgcc atcaagtgac tcgtgcccgt
     4261 ttggataaat aatgtccatt gatctttagg aatagcgcgt tgatcaccga tagcaagcgc
     4321 aatcaaaacc ccactataag gatgatccga tagtacttca ttaaaccgct tgcgaatctg
     4381 atcccgtaag cgctcgataa aataaaatgg tgtacgtact tcttcatcca gacgaatatt
     4441 ctggtctgaa taccgtacat aaccaatcgc tctaatatta cgttctaggt attttatttc
     4501 tgtatcgagc acataaggat tggcatttcc atgaggacgt ttcaaccgaa ctgtcaattg
     4561 ccatcgttct ccaggttgaa ttttgatcgc cctatgttga ttctggtacc aagtaattaa
     4621 aatatgcttc gggacataag cgccttccgt ttggatcttt tcaatatcga attgaaagcg
     4681 tatcccctgt cgagtgattt ctggcaattt tgcaatcacc ccggtcacag caatatcacg
     4741 actttcccat tcttctggca attcatctgc aagacgccaa tgcgcattaa cccctgccca
     4801 aagaaaacct aaggtaagaa aaaaacaaaa aataagaaac gcattgataa atcttgcgag
     4861 ccgagtactg taattagcaa acccagcaat caaaacgact aataacggca gtatccaaat
     4921 tgcactgctt tccggtaaag ttgcttgctg ctgcagccaa caaacaccag ccacgaatcc
     4981 taaactttta aaatgatttg gtaatttcgt aacgatttcg atcaacctag cttttgtaag
     5041 catggaaacg catgtgaaac atcacgtttg aaaataaaaa taaaggaagt tttaaaattc
     5101 acacgactga cattaacgcg aaatgacaat ttacggttaa aatgcactat ttaataataa
     5161 gaggctagtc atgttccgaa ttgctcccag tattttgtct gcaaattttg caaaactcgg
     5221 tgaagaaatc accaatgtat tagattccgg cgcagatatt attcacttcg atgtaatgga
     5281 taatcactat gtacccaatt taaccatcgg cccactcgtc tgtgaagcga ttcgcccact
     5341 cacaaaagca atgatagatg ttcatttaat ggtggagcct gttgatcgca tcgttcctga
     5401 tttcgctaaa gcgggcgctg atatcatttc atttcacccg gaagcttcta atcatatcga
     5461 tcgtacgatt ggtttaatca aagaacaagg ttgtaaagca gggttagtct ttaatccggc
     5521 tacgccattg agttacctag atcatatttt ggataaactc gacttgattt tgattatgtc
     5581 cgttaatcca ggttttggtg gtcaaaaatt tattcctgag gcactcaata agcttcgtgc
     5641 agttcgccaa cggattgaag ccagcggcaa gaaagatatt ttgcttgaaa tcgatggcgg
     5701 cgtgaaagtc gataacatcg ctgaaatcgc ccgtgcaggt gcggatacct tcgttgcagg
     5761 atctgccatc tatggcgctg gaaaggatag tgatcctcat cgctatgata cggtactcgc
     5821 tgccatgcgc agtgaattag caaaagtaaa agcttaaagt aaaccgcgtt aagggataac
     5881 tgttgaacgc attcaatcat aacaatcaat attctttatg acacattctg cttcctctca
     5941 acagatttat cttaaaaagg aaattgctaa cccattagcg attaaggctg tcgtcatcga
     6001 tcttgatgga actttgctgg ataccgccat ggatctggcg acagcagcca atctcatgtt
     6061 agtcgaatta ggcaaaccag aattatcatt tgcgaccatc cagacattta ttgggaaagg
     6121 gattcaaaag cttgttaagc gtgctttaac caatcatctc gatgttgaac ctgatccagc
     6181 cttatttgag aaagcgcttc caatttatga gcatcactat cgagaaaatc tcagcaaaca
     6241 gacccgacct taccctggtg tggtagaagg gttaaaaatc ctacaacaaa agggtttccg
     6301 tttagcttgt atcacgaata aagctgaagc atttaccgtg ccgctattaa aagcaaccca
     6361 tttattcgat tattttgagc ttgtcttatc gggcgatagt ttgccgagga aaaaacccga
     6421 tccactccct ttattacatg catgccaatt ttttcagatt gaaccgcatc aattattact
     6481 cattggagat tcattaaatg atgcgattgc agcgcgagca gcgggttgtg cgattttctg
     6541 cgtaccttat ggttataatg aagggcagga tgtaaaagaa ctcgattgcg acgcaatcgt
     6601 acccaccatt gctgctgcaa cagaaatcat tgagaacgca tagcaaccaa ctaatccagc
     6661 acgatttaac ctctatcata ctgcgaagca gcgtaaatca tgaatgaaga taaatttaac
     6721 gagctagcag cacaaggtta taactacatc cctttcgtgc tcgaaacctt cgctgattta
     6781 gacaccccgc tctcgatcta cctcaaatta gcgaatcaac ctttctccta tttacttgaa
     6841 tcggtactag gaggagaaag attcggtcgg tactctgtgg taggcttacc agccagaacc
     6901 cgtctagaag tttttaagca acaaatcaac atcattaagg gcgttgcaac agaaacccat
     6961 catgtaactg atgttttggg tttcattgaa tcttatttag aaaattataa aattgcaccc
     7021 aatgatgatt ttgatttgcc tcgattctat gggggattag ttggttattt tggttacgac
     7081 acgatacgct atatcgaacc caagcttagc aactgctcca aaccagatac cctcaacaca
     7141 ccggatatct tgttactctt atccgaagag ctcgtcgtta tcgataatct cgctggaaaa
     7201 atttatctca tcgtttttat caatcctaat accgatcacg cataccaaac cggtaaagat
     7261 cgaattaaag cgctgctaaa acagttacga caacccgtta caatcccacc tgaatcgcct
     7321 cattcaccta gcactgcaac cagtgaattt tcagaatcag cgtttatcgc agccgttgaa
     7381 cgtgccaaac aatacatcat cgatggtgat atcatgcaag tcgtgctatc gcagcgcacc
     7441 agcctaccct acacggcttc tccgcttgct ctgtatcgcg cattacgcag cttgaaccca
     7501 tctccttaca tgttttacta ccatttagga gatttccata ttgtgggcgc ttctcctgaa
     7561 atcctggttc gactcgagaa aaatgtggtt acagtacgtc ccatcgctgg cacccgtccg
     7621 cgcggcaaac atttccagga agatcaagcg ctcgcaaccg agctattagc tgatcctaaa
     7681 gaacgcgccg aacacgtcat gttgatggat ttggggcgca atgacattag ccgcgtcgcc
     7741 caaaccggca ccgtaaaagt aaccgaaaac atgaccatcg aacgatactc ccatgtaatg
     7801 catatcgttt ccaacgtcga agggcagctc gaacctaatt taactgcaat cgatgtgcta
     7861 cgggccactt ttccagccgg tacggttagc ggtgcgccta aaatccgggc gatggaaatc
     7921 atcgatgaac tcgaaatatc taagcgcggt atctacgcag gcgctgtagg ctacctcgga
     7981 ttcaatggcg atatggatct agccattgcc attcgcactg gggttattaa ggatggtaag
     8041 ctatatgttc aggccggcgc tggtattgtt gctgattcca ttccttataa tgaatgggta
     8101 gaaacgcaga ataaagcaaa agccgtgtta cgggccgcag aaattgctga aaaaggactc
     8161 gatagcaaaa taaactaacg acacgccttt caatttaaaa tgaatttgga gaagtgaccg
     8221 agaggcttaa ggtgcacgcc tggaaagcgt gtgtacgctt taaaacgtac cgcgggttcg
     8281 aatcccgcct tctccgccaa acacccatta tccatgtccg ggtattcgtc aagcacttga
     8341 ttcaaccaat atttcccatc cgactcacta cagcatttgg cgtggtaacg ttgatgctgc
     8401 agaaccggta tttacgagct gtgttggctt gcatgaaagc gtgatagtga tcatcgattt
     8461 taccgatctt ggggttctac tgtcacg
//
LOCUS       DAOQLJ010000101         8486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3332_length_8486_cov_5.871308, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000101 DAOQLJ010000000
VERSION     DAOQLJ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3332_length_8486_cov_5.871308"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..658)
                     /locus_tag="PK522_07885"
     CDS             complement(293..658)
                     /locus_tag="PK522_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNC41689.1"
                     /translation="MNKRFLSQLIISLGICSLLLIPMTVFAEEGTAVHYSNKFQGRKT
                     ASGEVFDQKKLTAAHKQYPFGTKVRVTNLENNRSVIVKINDRMATKNSNIIDVTSRAA
                     NELGFRKKGKAQVSLEAVD"
     gene            complement(789..1856)
                     /locus_tag="PK522_07890"
     CDS             complement(789..1856)
                     /locus_tag="PK522_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41690.1"
                     /translation="MGIEQLNNLIGKWVSGFSINVITDRTWVELASILAPSALLILIL
                     FGIAVSLENKTWRSFNLWICLLVTIFYLPYELYRQSSNLVKAQTNIGEVQTNLKLLLD
                     SAHLNHVGNLIDEEVSSHVLGEMIHSGEKKDLLYISWLLSENEKKLLAEQENKQKMIV
                     NELNSALSNLKQEIVETQEPVEKIANDILSRIDGTISQLIESKIQHFNQALNQSLENF
                     QSEINTFVQNELNTYQAKLTEITQQNTDELQSYTRSAKQTFADQINQTNKESLQKLDE
                     TTKSIDQMKAALGGINFEQVVGQVQQLSNSIKSAQERNEALFEYNECIRTAGIIDLIG
                     KEEDCKKKLNNRLNELTHSNK"
     gene            complement(1942..3408)
                     /gene="lpdA"
                     /locus_tag="PK522_07895"
     CDS             complement(1942..3408)
                     /gene="lpdA"
                     /locus_tag="PK522_07895"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNC41691.1"
                     /translation="MAKLFDVAVIGAGPGGYVAAIRCAQLGLNTVCIDEWKNSEGTPS
                     LGGTCLNVGCIPSKALLESSENFERAAKKFGEHGIKLENLSIDVPAMIARKNKIVKAF
                     TSGIAMLFKKNKITSMHGRGMLLDRQNENQLWNIQVSNGDQSEVVQAEFVILATGSTP
                     RPFPLVEIDNDKVLDNVGALALSEVPKRLGVIGAGVIGLEMGSVWRRLGAEVTILEAQ
                     PGFLLSADESVAKEAQKVFTRELGLVIQTGVDINSIKQNKQQISIAYTDSNKESQKLE
                     VDKLVVAIGRVPNSKGLASDGNSLSVNDQGFIEIDVHCRTNLPNIFAVGDVVRGPMLA
                     HKASEEGVAVAELIATVKKQAANNESLPKVDLTVVPWVIYTSPEIAWVGKTEQMLKAE
                     NIPYKVGQFPFMANGRARALGETHGFVKILAHAETDRILGVHMIGPFVSELIAEAVVA
                     MEFKASSEDLARIIHAHPSLSEVLHEAALGVDKRALHI"
     gene            complement(3462..4511)
                     /gene="nagZ"
                     /locus_tag="PK522_07900"
     CDS             complement(3462..4511)
                     /gene="nagZ"
                     /locus_tag="PK522_07900"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNC41692.1"
                     /translation="MSLGPLMLDIGSTQLTQADITRLKHPLTGGVILFARNFESSKQL
                     IELTAEIRSIRNPQLLIAVDHEGGRVQRFQKEFTRLPAMHELGKIWNHRPQQAKHLAK
                     QIGFVMSAELSACGIDFSFTPVLDVDHGQSSVIGDRAFHRSSSAIAELAHSLMIGLKL
                     NGMPAVGKHFPGHGFIKADTHHELAIDPRIYQDIEMEDLIPFKQLIDFGITGMMAAHV
                     IYPNVDKHPAGFSSYWLKTILRNELGFEGCIFSDDLSMKGTEGYGNIVDRTRISLEAG
                     CDMVLVCNDSLSCDLLLDQLKWETSAISLARLARMRGRSHASSLIKLHEEASFVQAVN
                     EIDQIGKDNLELSFT"
     gene            complement(4514..4891)
                     /gene="acpS"
                     /locus_tag="PK522_07905"
     CDS             complement(4514..4891)
                     /gene="acpS"
                     /locus_tag="PK522_07905"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNC41693.1"
                     /translation="MIYGIGTDLVKPSRITRSLEKYGERFARRLLSEWEWQDYVNNNQ
                     PALFLAKRFAAKEALSKAMGTGLRPPVTLTNIAIAHDSLGKPYFEFHPELATLIKNEG
                     ITHHHLSISDELNLATAFVILER"
     gene            complement(4888..5613)
                     /gene="pdxJ"
                     /locus_tag="PK522_07910"
     CDS             complement(4888..5613)
                     /gene="pdxJ"
                     /locus_tag="PK522_07910"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041353082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNC41694.1"
                     /translation="MVTLGVNIDHVATLRQARKTIYPSPVMAALIAESAGADAITLHL
                     REDRRHIQEYDVETLRQQLKIRMNLECAITEEMVTFACRIKPHDVCLVPERREELTTE
                     GGLDVVRYFKQVKTVCSRLADAGIFVSLFIDAELDQIETAVKAGAPAIEIHTGPYADA
                     KDSDDQQSELEKICAAVAYGNRLKLKVNAGHGLHYENTRPIAAIEGISELNIGHAIVS
                     HALFVGFEQAVKQMKTLIQEASQ"
     gene            complement(5647..6543)
                     /gene="era"
                     /locus_tag="PK522_07915"
     CDS             complement(5647..6543)
                     /gene="era"
                     /locus_tag="PK522_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNC41695.1"
                     /translation="MNNSPQFRTGYVAIIGKPNVGKSTLLNFLIQQKISITSKKVQTT
                     RHRIKGILTDQQSQFIFVDTPGYQVRYSNKLNTIMNKIVIQSINEVDVVLFVIEAINF
                     DSNDELILRQLPANKPVILVINKIDQLSDKSKLLPFLAQISTKYPFTEMVPISAIKKM
                     QLTQLLGAVRAYLPENPPLFGEDEICDRNERFLAAEMIREKLFRLMGDEIPYSASVSI
                     DRFEQLTHIRKIYACILVDKTSQKIMIIGQGGEKLKQIASLARKDMEKLFGSKVYLEV
                     WVKVKAGWANDAKTLSGLGYEG"
     gene            complement(6621..7343)
                     /gene="rnc"
                     /locus_tag="PK522_07920"
     CDS             complement(6621..7343)
                     /gene="rnc"
                     /locus_tag="PK522_07920"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNC41696.1"
                     /translation="MILTSSPNTSPKLSEKTRFNLFFETIGYSFKELTLLREALTHRS
                     FGLPNNERLEFLGDSVLNCAISGLLFKKFPTITEGDLSRIRSNLVNQSTLFNLALSIG
                     IADLIRLGEGEKKSGGNNRPSILANALEAILGAVFLESGFKKTETCIKKIYAPLLQTL
                     EFDAIDKDAKTTLQEHMQSLKLDLPEYIVLAVSGLAHRQKFMVECVIAKLNIRVVGEG
                     TSRRRAEQDAARQAYALATQSG"
     gene            complement(7340..7708)
                     /locus_tag="PK522_07925"
     CDS             complement(7340..7708)
                     /locus_tag="PK522_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HNC41697.1"
                     /translation="MYPSTSRKQQGISLSGLMIWSFVLILVSIVGMKLIPAYIENATI
                     KRTLLAVATESNLRTNNEEIRQSFNRRASIDNIKAINGQDIIIDKKSDKVVLSTSYSV
                     KIPLFANVSLYIDFTAASNQ"
     gene            complement(7742..>8486)
                     /gene="lepB"
                     /locus_tag="PK522_07930"
     CDS             complement(7742..>8486)
                     /gene="lepB"
                     /locus_tag="PK522_07930"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNC41698.1"
                     /translation="DFFILKHKRTVDSAEPWWVEYPKSFFPIILIVFCLRSFLVEPFK
                     IPSGSMIPTLLVGDFILVNKYIYGIRLPVINQKIIDIDLPKRGEVLVFRFPEDTSIDY
                     IKRVIGVPGDTVTYRNKQLSINGTPLKLEQDGDYKYIESGLAFIYAQRFKEINGTGSY
                     SIIINHDIPPLHLSAVHSFPYRDNCVFDRTGFTCNVPEGNYFTLGDNRDGSSDSRYWG
                     FVPEKNIVGKAFLVWWNFSDLSRIGLSIE"
BASE COUNT         2511 a         1835 c         1419 g         2721 t
ORIGIN      
        1 tactcaaaac actgcatggc ccgatcggca tgactgccac tgaaattatc gactcagcac
       61 gatgccttat taatcaatgg gtatatggca ctgatgtttc atcacgatac ttacgacttt
      121 tcctataaat tcatttgata gatattactt ttacctgctt aggtaaggtc atataaaact
      181 taagccctat attagcaaac ttaatctgct gatatagggc ttaatcaatt actaaagata
      241 atagaaattt acttgttagc ttcgactctt ccagctgatt cgtgctattt tattaatcaa
      301 ctgcttcgag gctaacttga gctttacctt ttttcctaaa accaagctca tttgctgcac
      361 gactggtcac atcaatgata ttgctatttt tagttgccat gcgatcattg attttaacga
      421 ttacactgcg gttattttct aaattcgtga ctctaacttt agtcccaaaa ggatattgtt
      481 tgtgtgcagc ggttaatttc ttttgatcaa agacttcacc acttgcagtt tttctgcctt
      541 gaaatttatt gctatagtga acagcagtcc cttcttcagc aaatactgtc ataggaatca
      601 atagcaaact gcaaatgcca agactaataa ttaattgtga tagaaaacgt ttattcattt
      661 tattattcct tctagtattg aattatttat tttactcagc cattctaacc actgttttaa
      721 actaaaacaa taaacctgtc atgacaagcg ttaatatccc taaaatgggt tgcttattag
      781 tttgcagctt atttgttcga gtgcgtcaat tcgtttagac gattatttaa tttcttctta
      841 caatcctctt ctttcccgat tagatcgatt attcctgcag ttcgaatgca ttcattgtat
      901 tcgaacaacg cttcatttct ttcttgcgct gattttatcg aatttgatag ttgctgtact
      961 tggcctacta cctgttcaaa gttgatacca ccgagagcag ctttcatttg atcaatactt
     1021 ttagtcgttt catcaagttt ttgtagagat tctttgttag tttgattaat ctgatctgca
     1081 aaagtctgtt ttgctgagcg tgtatagctt tgcaattcat ccgtattctg ttgtgtaatt
     1141 tcagttaatt ttgcttgata agtatttaac tcattttgta caaacgtatt aatttcactt
     1201 tggaaatttt ctaatgactg attcagtgct tgattaaagt gttgaatttt actctctatc
     1261 aactggctaa tcgttccatc gatacggcta agaatatcgt tcgctatttt ttctaccggt
     1321 tcctgggtct caacaatctc ctgctttaaa ttagatagcg ccgaattcaa ttcattgaca
     1381 atcatttttt gtttattttc ttgttctgcc aacaattttt tttcattctc gctcagtaac
     1441 cacgaaatat acaggaggtc tttcttctct ccagaatgga tcatttcccc gagcacatgg
     1501 gaagacactt cttcatcgat tagattgcct acgtgattca aatgggccga atcaagtagt
     1561 aattttaaat ttgtttgaac ttcaccaata ttcgtttgag ccttaacaag gttgctagat
     1621 tggcgataaa gctcataagg tagataaaaa atggtgacta ataaacaaat ccataaatta
     1681 aatgatcgcc aagttttatt ctcaagagaa actgcgatac caaacaaaat caagattaac
     1741 aatgctgagg gcgccaaaat ggatgcaagc tcaacccaag tcctatcggt tatgacgttg
     1801 attgaaaaac cactcaccca ttttccaatc aaattattca attgttcaat tcccattcgc
     1861 taccctcccc tatgaaatct cttctatttt ctaatattgt tgatattgat tatttaacca
     1921 atcgaaaata tttaacctac cctaaatatg taaagcacgc ttatcaacgc ctagcgcagc
     1981 ttcatgtaac acctctgaca atgaaggatg cgcatgaata atgcgcgcaa gatcttcact
     2041 actcgcttta aattccattg ctacaactgc ctccgcaatt aattctgaga caaaaggtcc
     2101 tatcatatgg acccctaaaa ttcgatcggt ttcagcgtgc gctaaaattt tgacaaaacc
     2161 atgtgtttca ccgagtgctc ttgcccggcc attcgccata aacggaaatt gacctacctt
     2221 gtagggtata ttctcagctt ttagcatttg ctccgtttta cctacccagg caatttcagg
     2281 cgatgtataa ataacccatg gaacaacggt cagatcaact tttggtaaag attcgttatt
     2341 agctgcttgt tttttaacag tagcaattag ttcagcgaca gcaacacctt cttctgatgc
     2401 tttatgggct aacatcggtc ctcttactac atcgcctact gcaaaaatat taggcaaatt
     2461 cgtacgacaa tgcacatcaa tctcgataaa accctgatca ttcacagata aactgttgcc
     2521 atcactggct aagcccttag aatttggcac tctaccaatt gcgactacga gtttgtcaac
     2581 ctcgagtttc tgtgattctt tatttgaatc tgtataggct atagaaattt gttgtttatt
     2641 ttgtttaatt gagttgatat caaccccagt ctgaatcacc aaacccaatt cacgggtgaa
     2701 gaccttttgt gcttctttag caactgactc atcggcactc aacaaaaaac caggttgtgc
     2761 ttcgagaatc gtaacttctg cacctaaacg acgccagacg cttcccattt ccaatccgat
     2821 aacaccggca ccaatcaccc caagacgctt aggtacctca gataaagcaa gtgcaccgac
     2881 attgtctaac actttatcat tatcaatctc gaccagtgga aaaggtcggg gggttgatcc
     2941 ggtcgctaat ataacaaact cagcttggac cacttcactt tgatcgccat tgcttacttg
     3001 gatattccat aattgattct cgttctggcg atcaagtaac attccccggc catgcatcga
     3061 ggtgatttta ttttttttga aaagcattgc tattccactg gtaaatgctt ttacaatttt
     3121 attcttacgg gcaatcattg ctggtacatc gatggaaagg ttttctaatt tgattccatg
     3181 ctcgccaaac tttttagcag cacgttcaaa attctccgaa gattccaata acgctttgga
     3241 tggaatacaa cccacattaa ggcaagttcc acccaaactc ggcgttcctt cgctattttt
     3301 ccattcatca atacatactg tattcagtcc aagttgagca cagcgaattg cagcaacgta
     3361 cccacccggt cctgcaccga taacagcgac atcgaataat ttagccatga tttcttttta
     3421 taaattaaaa acgattaggt ttaaagtgtt tgaaatatct tttaagtaaa gcttagttct
     3481 aagttatctt tgccaatttg gtctatttcg tttacagctt gaacaaagct ggcttcctca
     3541 tgtaatttaa tcagtgaaga agcatgagat cggccacgca tacgggctaa tcgggcaaga
     3601 ctgattgccg acgtttccca ttttaattgg tctagcagca aatcgcagga taaagaatca
     3661 ttacatacca ataccatatc acaacccgct tctagggata ttcgagtacg atcaacgata
     3721 ttgccatagc cttccgtacc tttcatactc aaatcatcgc taaatataca cccttcaaat
     3781 cccaattcat tgcgtaaaat agtttttaac caatagctgg aaaatcctgc tggatgttta
     3841 tcaacgttag gataaatgac gtgtgctgcc atcattcccg tgattccaaa atcaatgagt
     3901 tgtttaaagg ggatgaggtc ttccatctca atatcctggt agatccgagg atcaatcgcg
     3961 agttcatggt gcgtatctgc tttaataaat ccatgacctg gaaaatgttt tccaacagca
     4021 ggcataccat tcagttttaa accgatcatt aaactatgtg ccaactcagc aatagcagaa
     4081 gatgaccgat gaaatgcacg atcaccgata acactactct gtccatgatc cacatcgagt
     4141 accggtgtaa agctaaaatc aataccacag gcggacaatt cagcagacat cacaaaccca
     4201 atctgttttg ctaagtgctt cgcttgttgt ggacgatgat tccatatttt tccaagctca
     4261 tgcatggccg gtaatcgcgt aaactctttc tggaatcgct gtactctgcc cccttcatga
     4321 tcaaccgcaa tcaatagctg tggatttctt attgaacgaa tctctgcagt cagttctatt
     4381 aattgtttac tcgattcaaa attacgcgca aaaagaataa ctccaccagt taaagggtgc
     4441 tttaatctcg taatgtctgc ttgggtcaat tgcgtgcttc caatgtcaag catcagagga
     4501 ccaagtgaca tatttatctt tccagtatca caaaagcggt tgctagattc aactcatcgc
     4561 taatcgataa atgatgatgc gtgattccct catttttaat taacgtagcc aactctgggt
     4621 gaaattcaaa atagggttta cctaacgaat catgggcaat tgctatattc gtaagcgtaa
     4681 ctggggggcg cagacccgtc cccatcgctt ttgacagcgc ctctttcgca gcaaagcgtt
     4741 tagctagaaa aagggcgggc tgattattat tgacgtaatc ctgccactcc cattcagata
     4801 gtaagcgtct agcaaaacgt tctccatatt tctcgagaga tcgtgttata cgagaaggtt
     4861 ttacaagatc tgtgcctatg ccatagatca ttggcttgct tcctgaatta atgttttcat
     4921 ttgcttcaca gcttgttcaa agcctacaaa taaggcgtgc gatacaatcg catggccaat
     4981 attcaattct gaaatacctt cgatggctgc gataggacgg gtattttcat aatgtaaacc
     5041 atgcccggca tttaccttca gcttgagacg attgccatag gctaccgctg cacagatttt
     5101 ctctaattcg ctttgctgat cgtcagagtc tttagcatcg gcatagggtc ccgtatgaat
     5161 ttcaattgcg ggtgcgcctg ctttaactgc tgtttcaatt tgatccaatt cggcatcaat
     5221 aaacaatgaa acaaagattc cagcatccgc taatcggcta caaactgttt tgacttgctt
     5281 aaaataccga acaacatcta atcctccttc cgttgtcaat tcttcacgtc gttcaggcac
     5341 taaacataca tcatggggct taattcgaca tgcaaaagta accatctctt cagtgatcgc
     5401 gcactctaaa ttcatacgaa ttttaagttg ctgccgtaat gtttcaacat catattcttg
     5461 gatatgacgt cgatcttccc ttaaatgaag agtaatggca tcagcacccg ctgattcagc
     5521 tatcaacgca gccatgactg ggcttggata aattgttttc cttgcctgcc gcaaagttgc
     5581 gacatgatcg atattaacac ctagtgtaac cacttaagat ttagtcctat tattaaaata
     5641 gctaaattag ccttcatacc cgagtccact taacgttttt gcatcattgg cccaacctgc
     5701 tttcactttc acccaaactt ctaaataaac tttgcttcca aataattttt ccatatcttt
     5761 tcgagctagt gatgcaatct gcttcaattt ttcgccacct tgcccaataa tcattatttt
     5821 ttgactggtt ttatcaacca aaatacaagc ataaattttt cgaatatggg ttagctgctc
     5881 gaatcgatcg atcgaaacac tcgctgaata tggaatttca tctcccatta atcgaaacaa
     5941 cttttctctt atcatttcag cagctagaaa acgttcgtta cgatcgcata tttcatcttc
     6001 accaaataag ggaggatttt caggaagata ggctctaact gctccgagca gctgagtcaa
     6061 ttgcattttt ttgatcgcac tgatcggcac catttcggta aatggatatt ttgttgaaat
     6121 ttgagcaaga aaaggtagaa gttttgattt atcgctcaat tgatcgattt tattaattac
     6181 caatataaca ggtttattgg caggtaactg acgcaatatc aactcatcat tactatcaaa
     6241 attgatcgct tcaatgacga acaacacaac atcgacctca ttgatgctct gaatcactat
     6301 tttgttcatt atagtattta atttattcga atagcgaacc tgataacctg gagtatctac
     6361 aaaaataaat tgtgattgct gatccgttag aatccctttg atgcgatggc gcgtcgtttg
     6421 aactttctta gaagtgatac taattttttg ttggatcaaa aaattaagta atgtggactt
     6481 acctacattc ggtttaccaa taatcgcaac ataaccagtc ctaaattgag gtgagttgtt
     6541 cattaattgg gtatataggc tgtttatata tcgttttctt agaaggtttt gctagactaa
     6601 atcagattag tcgctgtcaa tcaacccgat tgtgttgcta gcgcgtaagc ttgccttgcc
     6661 gcatcctgct cagctctgcg acgactggtt ccttcaccca caactcttat attgagcttg
     6721 gcaatcacac attcaaccat aaatttctgg cgatgcgcca aaccacttac cgcaagcacg
     6781 atatattccg gtaaatcaag ttttaaactt tgcatatgtt cttgtaacgt tgttttagca
     6841 tctttatcaa ttgcatcgaa ttctagtgtt tgtaataaag gcgcgtatat ctttttaata
     6901 catgtttcag tttttttgaa gccgctttca agaaaaacag caccaaggat ggcctcgagc
     6961 gcatttgcta aaatagaagg tcgattatta ccaccgcttt tcttttcccc ctctcctaag
     7021 cggatcaaat cagcgattcc aatggataat gctagattga aaagtgtact ctgatttaca
     7081 agatttgacc ggattctcga taaatctccc tcagtgatcg taggaaattt cttaaaaagt
     7141 aatcccgata tagcgcaatt caatacgcta tcccctagaa actccaaccg ttcattattg
     7201 ggcaatccaa aacttcgatg tgtcagagct tcccgcagta aggttaactc tttgaatgag
     7261 taaccaatgg tttcaaaaaa aagattgaat cttgtttttt cagataattt aggagacgtg
     7321 ttaggagaag acgttaaaat cattgattac ttgcagcagt aaaatcaatg tataacgaaa
     7381 cattggcgaa caatggaatt ttcacagaat agctcgtgct cagtacaact ttatcactct
     7441 tcttatcgat aataatatct tgtccgttaa tcgctttaat attatcgata ctcgctcttc
     7501 tattaaaaga ttgacgaatt tcctcattat tcgttcttaa attagactcc gtagcgactg
     7561 caagtaaggt acgtttaatc gttgcatttt cgatatacgc agggattaat ttcatcccga
     7621 caatagatac taaaatcaat acaaatgacc aaatcattaa tcccgataag ctaattcctt
     7681 gctgctttct cgatgttgaa ggatacatat aactcctcat ttcaattaat aattaacttt
     7741 attattcaat cgaaagccca attctactga gatcactaaa attccaccaa actagaaatg
     7801 ctttcccaac gatatttttc tccggtacga atccccaata tcgactatca ctactgccat
     7861 cccgattatc acccaacgta aaataatttc cttcaggtac attgcaagta aaaccagtgc
     7921 gatcaaatac gcaattatct cgataaggaa aagaatgaac ggctgataaa tgtaagggag
     7981 gaatatcatg attgataatg atactgtaac tacctgttcc attgatttct ttaaatcgct
     8041 gtgcatagat aaacgcaagt cctgattcaa tatacttata atccccatct tgttctaatt
     8101 ttaggggagt accattgata ctcaattgct tatttcgata cgtaacagta tccccaggca
     8161 caccgattac acgcttaata taatctatcg aagtgtcctc tggaaaccta aacaccaata
     8221 cttcaccgcg cttcggcaaa tcgatatcaa ttatcttctg attaattacc ggtaagcgaa
     8281 taccataaat atatttgttt actaaaataa aatcgcccac caacaacgtc ggaatcatag
     8341 aacctgatgg gattttaaat ggctcgacta aaaatgagcg aagacaaaat acaatcaaaa
     8401 taattgggaa aaaactttta ggatattcga cccaccaagg ttcagccgaa tcaaccgtac
     8461 gtttatgttt aagtataaaa aaatca
//
LOCUS       DAOQLJ010000102         8370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3405_length_8370_cov_5.174023, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000102 DAOQLJ010000000
VERSION     DAOQLJ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8370
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3405_length_8370_cov_5.174023"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PK522_07935"
     CDS             <1..527
                     /locus_tag="PK522_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC41699.1"
                     /translation="LGGIGLNVIQGAKMVGADKIIGIDINPARESMAREFGMTHFINP
                     NDTENIVDAIVQLTDGGVDFSFECIGNTKVMRQALECTHKGWGRSIIIGVAEAGAEIS
                     TRPFQLVTGRKWEGSAFGGARGRTDVPKIVDWYMEGKIKIDPLITHTLKLDDINEGFE
                     LMKAGQSIRSVVIF"
     gene            559..1518
                     /gene="speB"
                     /locus_tag="PK522_07940"
     CDS             559..1518
                     /gene="speB"
                     /locus_tag="PK522_07940"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNC41700.1"
                     /translation="MVDKIKGDGAIRREGLYGSSIENAYAGVLSFLRRKYTRDLTGAD
                     IVVSGIPLDLATTFRPGTRFGPQAIRAASVQLVELRPYPWGFDPFDALAVIDYGDCWF
                     DAHNPLTIKPTIVEHAQRILTTNAKMLTFGGDHYISYPLLIAHAQKYGTPLALLHFDA
                     HCDTWPDNGPESLDHGTMFYKAIKENLINPSISAQVGIRTWNDDFLGMNIIDAPWVHD
                     QGVNATIKKILEIIGTNPVYLSFDIDCLDPAFAPGTGTPVPGGLTTDQALRIIRGLGS
                     LNIIGMDIVEVSPAYDHSSVTALAAAHIACDLLCLMRNQKLKI"
     gene            1524..2375
                     /gene="fghA"
                     /locus_tag="PK522_07945"
     CDS             1524..2375
                     /gene="fghA"
                     /locus_tag="PK522_07945"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HNC41701.1"
                     /translation="MTLPFEMRSTHRCFGGLQGFYQHRANSLGGLPMRFSVYQPPQAN
                     ANRVPVLYFLAGLTCTEETFMIKAGAQRLAAQYGYMLVSMDTSPRQTAIAGEADDWEI
                     GSGAGFYLDACLEPWSKFFHMESYVTKELYQLILEHFPADPTRIGIFGHSMGGHGALI
                     LALRNPSLYQSVSAFAPITAPSDCPWGQKAFNCYLGEAKDRWIQHDATALIAAGHKVP
                     TLLIDQGLNDPFLATQLNIDRFETTCQLAKQPLILRRHEGYDHGYYFISTFIEDHFKH
                     HETLLGG"
     gene            complement(2382..3608)
                     /locus_tag="PK522_07950"
     CDS             complement(2382..3608)
                     /locus_tag="PK522_07950"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HNC41702.1"
                     /translation="MIDKSTIKILILDDEPFMLKLLARMLANQGFVHVVCCNNGNDAL
                     AQLNVESTRPDLILLDLNMPEMDGIEFIRYLVERQFTGSLILVSGEDERMLKTTERLV
                     QAHKIAVLGYLQKPVKPDALLAMLAQWSPPSKAAPTKPRKTYSADDIRLAIVRGELVN
                     YYQPKVSVATGTVIGVETLVRWQHATDGLVFPNQFITVAESSGLINELTRLVLLNAFN
                     QAKRWQQSGMDLRVAVNVSMDNLASLDFQDLVVDLATQVGIPPNKIVLEVTESQLMRD
                     IRVSLEILTRLRLKRFHLSIDDFGTGHSSLAQLRDIPFDELKIDQGFVHQAWRDDTLR
                     VIYDASLALAKQLNMESVAEGVEDRQDWDFLRQTGCDLAQGNFISKPLLASEFGDWIT
                     YWQQRVKADLMVDNLS"
     gene            complement(3651..4694)
                     /locus_tag="PK522_07955"
     CDS             complement(3651..4694)
                     /locus_tag="PK522_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNC41703.1"
                     /translation="MDPLTHALSGVLIVRAVEPILPSLKLLPLRVRVVAGLVAAAFPD
                     IDIALRLIDTLIYLNWHQGPTHSLVLLPIWAFLLAHAFAWIHRGRFAWTLFYTPACLG
                     LAIHILGDWITAYGLMVFAPFSTERFFVPLAFVIDPWITGMIVAGLVMSNVFPRQTYI
                     AACALLCLALYGLLLTDKHDQAVKIGRDYVKNHSILDASVAVLPQPFSPFNWKIIVSE
                     NEKYHVLMVKLSDQSVFIPDMGLLSKMASAYTPVSTARWQKINRYGDVSAHAKLVREA
                     WFDPIFDDFRQFAVFPQFDKMDVYESNICVWFFDLRFQFPLLPPSFRYGVCRDKMGST
                     WTIERHRGIFWID"
     gene            complement(4748..5356)
                     /locus_tag="PK522_07960"
     CDS             complement(4748..5356)
                     /locus_tag="PK522_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC41704.1"
                     /translation="MTDPIEIRDAIIDTAVTLAAQKSWEAVRLYDVATALNLSLDQIR
                     PYFHEKEDLVDAWFDRADSAMLKQAEAAHFYELSVEQRIFQLIMGWLDALSSQRKVTR
                     QMILGKLEFGHLHIQIPGIMRISRTVQWIREAAHRDATSVQRALEESALTTIYLMTFI
                     YWMQDDSLGSTRTQQFLKQRLQQAFWVGELFFAKHHTDSSRA"
     gene            5591..6460
                     /locus_tag="PK522_07965"
     CDS             5591..6460
                     /locus_tag="PK522_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41705.1"
                     /translation="MKRQAKRLILSFCALGLLSNSYIALADTTSDTETLLNWAEKTFP
                     QFFKSKQATQSIEPWLYRFYPETGIYAGVNLTDNNVYTLGGPWGNNPALIDSLSNLIA
                     QINNSGGNGNIASCDTTNTIPGITYSQSGNVITVTSNGQCVTAPEQNATNLCPIPKQT
                     TPTGISILSTNTVTRSQISGITASIPGLLDPFLNLISASSNAKHCTVNAPNENTNLIV
                     NSDLCFDITTQLSDLIGVIEGITVNPPITLSTAGTYTSQAVSDCFATDAATITDAVTG
                     DLWVKENGSFVKK"
     gene            complement(6517..>8370)
                     /locus_tag="PK522_07970"
     CDS             complement(6517..>8370)
                     /locus_tag="PK522_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNC41706.1"
                     /translation="KESDVAQFRDWVQPKLVQGQRVEGIRDARPEIRAALERAEKFLS
                     LAALVSVIVAAAAIALSSRRFIQRHLDGCAVMRCLGASQTTLFSLYFYHLVLFGLVAS
                     TIGCVLGLIAQEFLSNWMAELVNSSLPWPSIVPAIYGLVMGVVLLLGFSLPPLLNLKS
                     VPALRVIRRDMGSVNLHSLTGYSLGLLVLALLFIWEAGDLRLGLMVVLGFIGAIAVFS
                     LLGWLLIQSLLLARRHNTNAWGYGLANIRRRIVTSVVQAIALGLGLMAMLVLTVLQND
                     LLKDWQMSLPPETPNRFLLNIQTDQLTSLTKFFDQHEMPEPTWYPMVRGRLTAINGHP
                     VQVENYKDIRKKQLLNREFNLSWSNELQSDNEITDGRWWSENQPMTESEISLEEEIAR
                     VIGVNIGDRLTFNIADQLFEATITNLRKVDWDTFRVNFFAIARPGYLENYPTYYVTSF
                     HLPADKTKLLHELIREYPNLLIIDVASVIEQIQKMIFQVSRAIEFVFIFTLLAGFVVL
                     YAAIVATQDERVYEAAIFRTLGAKRNQLVRAWASEFMILGALVGIFAAAGATVLGYVI
                     GKHALHIPYSFNPWIWLIGLLIGIVGVMVAGLIGTRFTLAQPPLLTLRRLG"
BASE COUNT         2505 a         1939 c         1661 g         2265 t
ORIGIN      
        1 gtttaggtgg aattggttta aatgtcatcc aaggcgctaa gatggtgggc gcagataaaa
       61 ttatcggtat cgacattaat ccagccagag aatccatggc cagggaattt ggaatgacgc
      121 acttcatcaa tccgaacgac actgaaaata tcgtcgatgc cattgtacaa ctcactgatg
      181 ggggagtcga ttttagcttc gaatgtatcg gtaacactaa agtgatgcgc caggcattgg
      241 agtgtaccca caaaggttgg gggcgaagca ttattattgg tgttgctgaa gcgggtgctg
      301 aaattagtac tcgcccattc caattggtaa cgggtagaaa atgggaagga tccgcttttg
      361 ggggcgcacg gggacgaacg gatgtaccta aaatcgtcga ttggtatatg gaaggaaaaa
      421 tcaaaattga tccacttatt acccataccc tcaaacttga tgatatcaat gaaggctttg
      481 agctgatgaa agccggtcaa tcgattcgtt ctgtcgtaat cttttaatcg taaaccgttt
      541 ttacgcaatc gagtctaaat ggtcgacaaa attaaagggg atggcgcaat tcgtcgtgaa
      601 gggctctacg gaagctcgat cgagaatgct tatgctggag tgctgtcttt tctacgccgg
      661 aaatatactc gtgatttaac tggtgctgat attgtcgttt ccggaatccc tttggattta
      721 gcgacgactt ttcgtcccgg cacgcgcttt ggaccacagg cgatccgagc tgcaagtgtg
      781 caactcgttg agctgcgccc ctatccttgg ggattcgacc catttgatgc gcttgcagtc
      841 attgattatg gtgattgctg gttcgatgct cataatccac ttacgatcaa accgaccatt
      901 gttgagcacg ctcaacgtat tctaacaacc aatgccaaaa tgctcacatt tgggggtgat
      961 cactacattt cttacccatt gctgattgct catgcacaga aatatggtac cccgttggct
     1021 ttacttcatt ttgatgcgca ttgcgatacg tggcctgaca acggaccaga aagtctcgac
     1081 cacggcacca tgttttataa agctatcaaa gaaaacttga tcaatccaag tatttcagcc
     1141 caggttggaa ttcgtacttg gaatgatgat tttctaggta tgaatatcat cgatgcccct
     1201 tgggtgcatg atcagggtgt caatgctacc attaaaaaaa ttctagagat tattggaacg
     1261 aatcctgtct atctcagctt tgatattgat tgccttgatc ctgcctttgc accgggcact
     1321 ggcacaccgg tacccggtgg actaacaacc gatcaagcac ttagaatcat tcgtggtctg
     1381 ggttctttga atattattgg tatggatatc gttgaagttt cccctgcata tgatcatagt
     1441 agcgtgactg cacttgcagc agcacatatc gcatgtgatc ttttgtgctt gatgcgcaat
     1501 caaaaattaa aaatttaatt ccgatgaccc ttccttttga aatgcgtagt acgcatcgtt
     1561 gctttggtgg tttacaaggc ttttatcaac accgagctaa ttcactcggt ggactaccca
     1621 tgcgattctc ggtctaccaa cctccccagg caaatgcaaa tcgagtccca gttctttatt
     1681 ttctagctgg acttacctgc acggaggaaa cctttatgat caaagcaggt gcgcaacgac
     1741 tagctgcaca atatggatat atgctcgtta gcatggatac cagcccgcgt caaactgcaa
     1801 ttgctggtga agcagatgac tgggaaattg gctcgggagc aggattctac ctggatgctt
     1861 gtttggaacc ctggtctaaa ttcttccata tggaaagtta tgtaaccaag gaactgtatc
     1921 agttgatatt ggaacatttt ccagcagatc caacgcgtat cggtattttc ggtcattcga
     1981 tgggaggaca tggcgctttg atccttgcat tacgtaatcc gagtttatac caatctgtat
     2041 ctgcatttgc ccccattacc gcaccttccg attgcccatg gggccaaaaa gcttttaatt
     2101 gctatcttgg agaagcaaag gatcgctgga ttcaacatga cgccacggct ttaatcgcag
     2161 caggccataa agtccctacg ctattgattg atcaaggact aaatgatcct tttctcgcga
     2221 cacaactcaa cattgaccgt tttgaaacaa cctgtcagtt agccaaacag ccgcttatcc
     2281 tgcggcgcca tgaaggctac gatcacggct attatttcat tagcacattt attgaagatc
     2341 atttcaagca tcatgagact ttacttggtg gatagctttg actaactcag attatccacc
     2401 atcaaatccg cttttacgcg ctgctgccaa tatgtgatcc aatcaccaaa ttctgaagct
     2461 agtaaaggct ttgaaataaa attaccttgt gcgagatcac aaccagtttg acgcaagaaa
     2521 tcccagtctt gtcgatcttc aacgccttct gcaaccgatt ccatattgag ttgcttggct
     2581 aaagcaaggc ttgcatcata gatcacacgc aaagtgtcat ctcgccatgc ttgatgaaca
     2641 aagccttgat cgatcttgag ctcatcgaaa ggaatatcgc gcaactgtgc aagtgaagaa
     2701 tgcccagtcc caaaatcatc gatcgacaag tgaaaccgtt tcaaacgcaa acgggtcaaa
     2761 atttcaagtg atacccgaat gtcgcgcatc aattggcttt cagtgacttc caatacgatt
     2821 ttattgggtg gaatgccaac ttgggtagcc agatccacaa caagatcttg gaaatctagc
     2881 gatgccaaat tatccattga tacatttacc gctacacgta aatccattcc cgattgttgc
     2941 catcgcttcg cctggttaaa tgcatttaat aaaactaacc gcgttaactc attaatcaat
     3001 ccactcgatt cagctactgt aataaattga ttaggaaata ccaaaccatc ggtagcatgc
     3061 tgccaacgca ccaatgtttc cactccaatc actgtccctg tagcaactga aaccttaggt
     3121 tgataataat taaccaattc acccctcaca atagctaatc taatgtcatc tgcgctatag
     3181 gtttttcttg gtttagtcgg agcagctttt gagggtggtg accactgtgc tagcatcgca
     3241 agtaacgcgt cgggtttaac gggtttttgt aaataaccga gtacagcaat tttatgcgcc
     3301 tgcacgagcc gctcagtcgt ttttagcatt ctttcgtctt caccgctaac aagaattaaa
     3361 ctaccggtaa attgacgctc aacaagataa cgaatgaatt caatgccatc catttccggc
     3421 atgtttagat ctaataaaat caaatcaggt cgtgtcgatt caacattaag ttgcgctaag
     3481 gcatcattcc cattattaca gcaaaccacg tgaacgaacc cttgattggc tagcatacgg
     3541 gccaataatt tcagcataaa gggttcatca tcaagaatca gtatcttgat cgtagattta
     3601 tcgatcatat tattcctttt cggtgtgact cagtcagcta aagaaggtat ctaatcaatc
     3661 cagaatatgc ctcgatggcg ttctatggtc caagttgaac ccatcttgtc ccgacaaaca
     3721 ccatagcgaa atgaaggtgg caatagcgga aattgaaagc gcaaatcaaa aaaccacaca
     3781 cagatattcg attcgtacac gtccatttta tcgaattgtg gaaataccgc aaattgacga
     3841 aaatcatcaa aaatcggatc aaaccaggct tcccgaacta actttgcgtg cgctgaaacg
     3901 tctccatagc gattaatttt ttgccaacgt gccgttgaaa cgggagtgta agcactcgcc
     3961 atctttgaaa gtaaccccat atcaggaata aataccgatt gatcggagag tttcaccatt
     4021 aacacatgat atttctcatt ttcagaaaca atgattttcc agttaaacgg cgagaaaggt
     4081 tgtggtaata ccgcaactga agcatcgaga atcgaatgat ttttgacata gtctcttcct
     4141 atttttaccg cttgatcatg cttgtccgtc aacaataatc catataacgc taggcacaac
     4201 agcgcacaag cagcgatata agtttggcga ggaaacacgt tgctcataac aagccctgcg
     4261 acaatcattc ctgttatcca cggatcgatt acaaatgcca gtggaacaaa aaaacgttct
     4321 gtactgaaag gagcaaacac catcaaacca taagcagtaa tccagtctcc taaaatatgt
     4381 attgctaacc ctaagcaagc cggtgtatag aaaagtgtcc aagcaaatcg gccacggtga
     4441 atccaagcaa aagcatgtgc taataaaaat gcccagattg gtaacaatac taacgaatgc
     4501 gtcggtcctt gatgccaatt caaataaatg agcgtgtcga taagtcgtaa tgcgatatcg
     4561 atatctggga atgctgctgc taccaggcca gctacaactc gtactcgtaa aggtaataat
     4621 tttaagctgg gcagtatagg ttccactgct cgcacgatga gtacaccgct tagtgcatgc
     4681 gtaagtgggt ccatcttaaa gtatgatgag ttcgttctat cattacgcga tattttgata
     4741 agtcagatca tgctcgactg gaatcggtat gatgttttgc aaaaaaaagc tcaccaaccc
     4801 agaaagcttg ctgaagtctt tgctttagaa attgctgagt acgcgtcgac cctaaggaat
     4861 catcctgcat ccaataaata aaagtcatca aataaatcgt tgtcaatgct gactcttcta
     4921 atgctcgctg cacagaagtt gcatcgcgat gcgctgcttc acgaatccat tgtactgttc
     4981 ggctaatccg cattattcct ggaatttgga tatgaagatg cccgaattcc agtttaccca
     5041 gaatcatctg tcgtgtgacc ttacgttggc tagacaaagc atctaaccac cccatgatta
     5101 actgaaaaat ccgttgctca acggatagct cataaaaatg agctgcttca gcttgcttta
     5161 acattgcgct atcagcacga tcgaaccaag catcgaccaa atcttctttt tcgtgaaaat
     5221 agggacgtat ctgatccaat gaaagattta gagcagttgc tacatcatat aaacgcacgg
     5281 cttcccatga tttttgggcg gcgagtgtca ctgctgtatc aatgattgca tcacgaattt
     5341 caatcggatc tgtcatctta gtctccgcaa ataagccatt agtaagccca ctttttctta
     5401 taattcaaca atcaattcga ctggactgta tttgtattgt tgctatttta tctagcaaat
     5461 gcagaaaata tatcgtttta accatttaaa tcactcgctt gcttttttta tgtaaatgcg
     5521 aacatagtcg atatttttct attagaatca aatcgagatt tacatgttac agttaaatgg
     5581 agaactaaat atgaaacgcc aagccaaacg actcatttta tctttttgtg ctctgggtct
     5641 actcagtaac agttatatcg cactcgcgga tactaccagt gataccgaaa ccttgttaaa
     5701 ttgggcggaa aaaaccttcc ctcaattttt taaaagcaaa caggcaacac agagtattga
     5761 accctggttg tatcgcttct acccagaaac gggaatctac gccggtgtca atttaaccga
     5821 taataatgtt tatacactag ggggtccatg gggaaacaat ccagcgctga tcgattcctt
     5881 atcgaatctc atcgctcaaa ttaataattc aggcggaaat ggaaatattg caagttgtga
     5941 cacgaccaat accattcctg ggattaccta tagtcagagt ggcaatgtca ttacggtcac
     6001 atcaaacggt caatgtgtta ctgctccgga gcaaaatgca actaatctat gccccattcc
     6061 caagcaaacg acaccaaccg gcatatcaat tcttagcacc aacacggtga ctcgttcgca
     6121 aatttcagga attacagctt cgattccagg actattggat ccatttttaa atctaattag
     6181 tgcttcttca aatgcaaaac actgtacggt gaatgcaccc aacgaaaaca ccaatctcat
     6241 tgtcaactca gacctttgct ttgatattac cacgcaactt tctgatctca ttggtgtgat
     6301 tgaaggaatt actgtaaatc caccgattac attgtcaact gcaggaacct acaccagcca
     6361 agctgtttcc gattgtttcg caactgatgc agcaacgatc actgatgcag taacaggaga
     6421 cctttgggtt aaggaaaatg gtagttttgt taaaaaataa cgctattaat taattcacgc
     6481 acccatccat caagcgctga ttgatggatg ggatcattat cctaatcgcc gcaatgtcaa
     6541 taatggcggt tgagcaagcg taaaccgtgt cccaatcagt cctgcaacca tcaccccaac
     6601 tatcccaatt aataacccga tgagccaaat ccaaggatta aagctatacg gaatatgcag
     6661 cgcatgttta ccaattacat agcctaatac cgtagctccc gctgcagcaa aaataccgac
     6721 taaagcaccc agtatcataa attcgctggc ccaagcgcga acaagctgat tgcgctttgc
     6781 acccaaggta cgaaaaattg ctgcttcata tacacgttca tcttgggttg caacaatcgc
     6841 agcatagaga acaacaaatc cagctaataa agtaaagata aacacgaatt caatcgctct
     6901 tgacacttga aagatcattt tctgaatttg ttcgattacc gacgccacat caataatgag
     6961 caaattagga tattctctaa ttaactcatg aagtagcttg gttttgtcag caggcaaatg
     7021 gaagctagtt acgtaatagg ttgggtaatt ctccaagtaa ccaggccgag caatcgcaaa
     7081 aaaattgacc cggaatgtat cccaatcgac cttacgtaga ttggtaatgg ttgcttcgaa
     7141 caactggtct gcaatattaa aagtcaaacg atcaccaatg ttcacgccaa tgacacgagc
     7201 aatttcctct tccagcgata tttcactctc agtcatcggc tgattttcag accaccagcg
     7261 cccgtccgta atctcgttat cagactggag ttcattcgac caggaaagat tgaattcacg
     7321 attcagcagt tgcttctttc ggatatcttt ataattctcc acttgaaccg gatgtccatt
     7381 gattgctgtc aatcgccctc tcaccatggg ataccaagtt ggttcaggca tttcatgttg
     7441 gtcaaagaat ttagtcaatg atgtcaattg atcagtttgg atattgagca aaaaccgatt
     7501 cggggtttca ggcggaagac tcatctgcca atcttttaac agatcatttt gcagcacggt
     7561 caataccagc atcgccatta agcctaatcc tagtgcaatt gcttgcacca cactggtcac
     7621 aattcgtctg cgaatgttag caagcccata gccccaagca ttggtattgt gtcgccgagc
     7681 gagcaacaat gactgaatca gtaaccaacc cagcaagcta aaaactgcaa ttgcaccgat
     7741 aaaaccaagt actaccatca gccctagacg caaatcccca gcttcccaga taaacaaaag
     7801 cgctaacacc aataatccca aactatagcc ggtcaaacta tgcaaattaa cactccccat
     7861 gtcacgccga atgactcgca atgcaggtac acttttcaaa ttgagtaacg gtggtaatga
     7921 aaaacccagc aatagtacga ctcccataac caatccataa atagcaggaa cgatacttgg
     7981 ccaaggaagt gatgagttaa ccaattcagc catccaattt gataaaaatt cttgagcaat
     8041 caatcccaaa acacacccga tcgtacttgc aaccaggcca aacaacacca aatggtaaaa
     8101 ataaagactg aacaaagttg tctgactcgc ccctagacaa cgcatcacag cacatccatc
     8161 taaatgacgc tgaataaagc gtcgagaaga taaagcaatg gctgcagcgg cgacaatgac
     8221 acttaccaaa gcagcaaggc taagaaattt ttcagctcgc tccagcgcag cacgtatctc
     8281 gggtcgggca tcgcggatac cttctacacg ttgaccttga accaatttag gttgtaccca
     8341 atctctaaat tgggcgacat cactctcttt
//
LOCUS       DAOQLJ010000103         8302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3455_length_8302_cov_5.690554, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000103 DAOQLJ010000000
VERSION     DAOQLJ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8302
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3455_length_8302_cov_5.690554"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..109
                     /locus_tag="PK522_07975"
     CDS             <1..109
                     /locus_tag="PK522_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HNC41707.1"
                     /translation="EIIIGKQRNGPIGKVDLTFLGEYTRFENYAKPDYY"
     gene            complement(135..521)
                     /locus_tag="PK522_07980"
     CDS             complement(135..521)
                     /locus_tag="PK522_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNC41708.1"
                     /translation="MTRRLISSGSTFEEEIGYSRAVVEGDWIFVSGTTGFDYSTMSIS
                     EDLLEQAEQCFKNIELALKQANSSLKDVVRVTYVLPEASDFEKCWPIFRKYLGDVRPA
                     AMMISAGLSDPRMRIEIQVTARKCVS"
     gene            867..1271
                     /gene="cheY"
                     /locus_tag="PK522_07985"
     CDS             867..1271
                     /gene="cheY"
                     /locus_tag="PK522_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HNC41709.1"
                     /translation="MSADKNLKFLVVDDFSTMRRIIRNLLRELGFLNVEEAEDGAAAL
                     RKLRESSFDFVVSDWNMPNMDGLTMLQSVRADEELKQIPVLMVTAEAKKENIIAAAQA
                     GANGYIVKPFTAATLDEKLNKILQNIAHKAAS"
     gene            1307..1990
                     /locus_tag="PK522_07990"
     CDS             1307..1990
                     /locus_tag="PK522_07990"
                     /inference="COORDINATES: protein motif:HMM:NF016247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HNC41710.1"
                     /translation="MNNPTNSSPTEISADDKLLERIGKLTRLLHNSICELGYDKDKKL
                     QEASEQIADIHERLNYVLSKTQSAAETTLSAIENIKPIQSNLSSSAIILTQEWQELIK
                     LENSCHSNKNVAKFSNLFMRTVSYLENIPKQAEVTNDYLVTVIMAQDFQDLTGQVIKK
                     ISHLLLLIENELIQVLLDNSISTNPATINNKSSPNNLLNGPTINPQKKPEILSNQTDV
                     DNFLADLGY"
     gene            complement(2015..2614)
                     /gene="coaE"
                     /locus_tag="PK522_07995"
     CDS             complement(2015..2614)
                     /gene="coaE"
                     /locus_tag="PK522_07995"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646453.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNC41711.1"
                     /translation="MIVGLTGGIGSGKTSVAEIFQTLGITIIDTDEIAHELTHENGEA
                     ISHIKEIFGGEYITDTGRLDRVKMRNLVFTDLSMKKKLEEILHPLIYRNVEQLIPTIE
                     SLYGIVAIPLLFETGCFCNLIDRVLVVDCPEELQILRTVQRSKLSQKDVRAIIKTQIS
                     RKERLVRANNIISNDRDFIYLVDQVRKLHSEFTSFTKMK"
     gene            complement(2627..3487)
                     /locus_tag="PK522_08000"
     CDS             complement(2627..3487)
                     /locus_tag="PK522_08000"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNC41712.1"
                     /translation="MAFINSLQASPELFVTICGVLGITVGSFLNVVIYRLPKMMELAW
                     HQQCAELKGEPAKLLPVFNLITPRSNCPHCGHKITMLENIPLISYLFLRGRCSQCHEH
                     ISFCYPTVELLAGLMSGFAAWYYGYSFMCFAALIFIWAMIALTFIDFDTQLLPDAITL
                     PLLWIGLLINLNNGIIDLQSAVIGAVAGYLVLWVVYWIFKLITGKEGMGYGDFKLLAA
                     IGAWFGWQTLPLVILSSSFVGGLVGIFLIYFRNQHSGLAIPFGPYLAGGGLIAMFWGK
                     QINHSYLSLF"
     gene            complement(3523..4746)
                     /locus_tag="PK522_08005"
     CDS             complement(3523..4746)
                     /locus_tag="PK522_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNC41713.1"
                     /translation="MATVAASNDKKVKEISFTWEGKDKTGKLIKGEMRAAGVEVVNST
                     LRRQGIRVTKVAKTKSSGSISDKDITLFTRQLATMMKSGVPLLQAFDIVGKGHSNPAV
                     GKLLMDIKLDVETGSSLANAFRKYPLYFDSLFCNLVAAGEAAGILDSLLDRLATYKEK
                     IQAIKGKIKSALFYPISIIVVAFVITAVIMIFVIPAFKELFQGFGADLPGPTLMVMTI
                     SDFFVDYWWAIFGGIGASLYSFFYAWKRSIAMQRVMDKLLLRLPIFGDVIRKATVARW
                     SRTLSTMFAAGVPLVEALDSVAGAAGNYVYYEATKHIQSEVSTGSSLVAAMTETQVFS
                     SMVLQMVAIGEEAGSLDAMLSKVADFFEAEVDNAVEALSSLMEPMIMVVLGTLIGGMV
                     VAMYLPIFKMGQVVG"
     gene            5019..6281
                     /locus_tag="PK522_08010"
     CDS             5019..6281
                     /locus_tag="PK522_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HNC41714.1"
                     /translation="MSDPTFIQLTASILFALAVIHTFSTKYFEHLAHIQPQHAGIWHL
                     LGEVEVVFGFWAMILILFMFAVGGKTEAVGYLNSRDFTEPMFVFVIMVIAGTKPILSF
                     TAFIVQRTANLLPINRAMAMYFLVLALVPLMGSFITEPAAMTLAALMLRDTLFGQTIS
                     NKLKYATVGVLFVNISIGGTLTPFAAPPVLMVAAKWNWDFWFMITTFGWKSAIAVMIN
                     AALVTLLFSKELVRTKQQDTDTTTTKMPALMIAAHITFLTLVVLFAHYPVVFMGLFLF
                     FLGFATAYQRYQNPLILREALLVAFFLGGLVVLGGQQQWWLQPLLMGMDDTAVFFGAT
                     ALTAITDNAALTYLGSLVEGLSEEFKIALVAGAVTGGGLTVIANAPNPAGFAILKSKF
                     DEQAIHPLGLLIAALLPTMIAIIAFRIL"
     gene            complement(6338..>8302)
                     /locus_tag="PK522_08015"
     CDS             complement(6338..>8302)
                     /locus_tag="PK522_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC41715.1"
                     /translation="MMVDPTVVVVENIYQRLGEAKDTGKSKFNVIVDAVAEVGMPVIF
                     GLIVTVLVFLPLMTLEGMEGKTFSPLAITISISLFIALLVSLLLSPVLCDYLIKGGGE
                     QDTKIIAVMKSAYLSVFDLAMRNQKKTIMIAISSLMLAFAIFPFLGKSFIPIMKEGAI
                     TPVIIRAPSISLEEAIAIETEAMKLIAAVPGVKSVVSKLGRGDTPADPAAQNESDPIA
                     DLDLKGSGRTQQEIEEDIRKALDGLPGVNIVLSQPIAQRVDEMLTGVRSQVAIKIFGD
                     DLEQLRKLSEQMARIVKSTPGARDIRIERLSGQQELTIDIDRRAIARHGLNVSDVNEI
                     ISTAIGGKFVTQVFEGERRFNLLLRFPEGFRHDVEAIKQILLRPANNNIGGEVATGGV
                     LVPLSAVADIHVKDGPAIVSREFARRRVVVGVNVHGRDLGGFVAELQERAAKEIKLPQ
                     GYYFVWGGQFENMERAMAKLSIIVPITLAAIFFMLFMLFNSVKLACLIYLVLPFASVG
                     GVVGLFVFREYLSVPASVGFIAVWGTSILNGVVLISFVKELREKGLSIEESVRKACSQ
                     RFRPVMMTAATTVLGLAPFLTATGLGSEIQKPLAVVVIFGLTTATCMTMIVMPMIYRW
                     FDTPPKRDLADSFEGVTAPAVEAVAQTKKA"
BASE COUNT         2752 a         1817 c         1347 g         2386 t
ORIGIN      
        1 tgaaattatt attggtaaac agcgcaacgg cccaattgga aaggtcgatc taactttctt
       61 aggggaatac acgcgctttg aaaattatgc gaaaccagat tattactaat atagtttgat
      121 ttcgtaaatt gccactacga aacacatttt cttgcggtta cttgaatttc aattcgcatt
      181 ctcggatctg ataatcctgc tgaaatcatc attgctgctg gacgaacatc tccaagatat
      241 ttacggaaaa taggccagca tttctcaaaa tcagaagcct ctggcaatac ataagttacc
      301 cttacaacat cttttaaact tgaattagcc tgcttcaatg ccagctcgat atttttaaaa
      361 cattgctcag cttgttctaa taaatcttcc gaaatactca tggttgaata atcaaaacca
      421 gtcgttcctg acacaaaaat ccagtctccc tcaacaaccg ctcttgaata accaatctct
      481 tcttcaaatg ttgaaccaga actaattaat cgccttgtca ttaagtttcc tctacttctc
      541 aaaataaaat tattatttcg cgattataca agtaaagatt taccttgata cagtattcag
      601 taagattaaa tctgcttaac cttgttaaat ttattgttat taagcaaaag ctggtaggca
      661 taataaaatc tctctaacca gcaataccta tttcataaaa tgatcagcag tataattctt
      721 aaagaactag aggggtatca ccaggagata actttcagca agtgcgcgat ctattactaa
      781 atttttattt tgataagccg taacacttta taagttaatc agtacaaaac gtactaattt
      841 agattctctc gtaaaaggat atactaatgt cggcagacaa gaacttaaaa tttttagtag
      901 ttgatgattt ctctaccatg agacgaatca tacggaacct tctcagggaa ttaggctttc
      961 ttaatgtaga agaagcggaa gatggagctg cagctctaag aaaacttcgt gaaagtagtt
     1021 ttgattttgt tgtgtccgat tggaacatgc ccaatatgga tggacttaca atgttacaaa
     1081 gtgttcgagc agatgaagaa ctaaaacaaa ttcctgtttt gatggtaaca gctgaggcta
     1141 agaaagaaaa cataattgca gccgctcaag ctggtgcgaa cggttatatc gttaagcctt
     1201 ttactgcagc aactctagat gaaaaattaa ataaaatcct acagaatatt gctcataaag
     1261 cagcttcgta aagaaagcct actttaccga tcgatttaag aaagcaatga ataatcctac
     1321 taactcttca ccaactgaaa tatcagctga tgataagcta cttgaaagaa taggaaaact
     1381 tacaagactc cttcataaca gcatttgtga acttggatat gataaagata aaaaattaca
     1441 agaagctagt gagcaaatag cagatatcca tgaaagattg aattatgttt tgtctaaaac
     1501 acaatcagca gctgaaacaa cactgtccgc tatcgaaaat attaaaccaa tacaatcaaa
     1561 tctatctagt agtgcaatta tcttaactca agaatggcaa gagttaatta aactcgaaaa
     1621 cagctgtcat tcaaataaaa atgtagcgaa atttagcaat ttattcatga gaacagtatc
     1681 ttacttagaa aatataccta aacaagcaga agtaactaat gattacttag taactgtaat
     1741 aatggctcaa gattttcagg atttaactgg tcaagtaatt aaaaaaattt ctcatttact
     1801 gctgctaatc gaaaatgaat taatacaggt attactagat aactcaatat caaccaaccc
     1861 tgcaaccata aataacaaaa gctctccaaa caatttactc aacggaccta ccatcaatcc
     1921 tcaaaaaaaa cctgagatcc tctctaatca gactgatgtt gataacttct tagcagactt
     1981 aggttattaa agaaataaaa gaattcaagt tattttattt catttttgta aagctggtaa
     2041 actcactatg aagttttcta acttgatcta ctaaataaat aaaatcccga tcattagaaa
     2101 tgatattgtt agctctgacc aaacgttctt ttcttgagat ttgcgtctta attatcgctc
     2161 gaacatcttt ctgggaaagc ttacttcgct gaactgttcg taaaatttgt aactcttctg
     2221 gacaatcaac aacgaggact cgatcgatta aattacaaaa gcaacccgtc tcgaacaaca
     2281 gtggtatagc tacaatccca tataatgact caatcgttgg aataagttgt tctacatttc
     2341 tataaataag cggatgcaat atttcctcaa gcttcttttt catagataaa tctgtaaata
     2401 caagatttct catttttacc ctatctaagc gcccagtatc agtaatgtat tctcccccaa
     2461 aaatttcctt tatatgagaa attgcttctc cattctcatg agttaactca tgagcaattt
     2521 catcagtatc aataatggta atacccaatg tttgaaaaat ctcagcaaca ctggtttttc
     2581 cagaaccaat accaccagtc aatccaacga tcaagggtaa ttccttctag aacagactga
     2641 gatagctatg attaatttgc tttccccaga acattgcaat taatccacct cccgcaagat
     2701 agggtccaaa tggaattgct aatccgctat gctgatttcg aaaataaatt agaaaaatac
     2761 caactaaacc accaacaaat gatgaagata aaataacgag gggcaatgtc tgccacccaa
     2821 accaggcgcc aattgcagca agaagtttaa aatcaccgta gcccatccct tctttaccag
     2881 taattagttt aaaaatccag taaactaccc aaagtactag ataaccggct acagcaccaa
     2941 taactgctga ctggagatca ataatcccat tatttagatt aatcagaaga ccaatccaca
     3001 ataatggcaa tgtgattgca tctggcaaga gctgtgtatc aaaatcgata aacgtaagtg
     3061 caatcatagc ccaaataaaa ataagagcag cgaaacacat aaaactatac ccataatacc
     3121 atgcagcaaa accactcatt aatcctgcta atagttcaac tgttgggtaa caaaaagaaa
     3181 tatgctcatg acattgagaa cagcgacctc ttaaaaacaa atagctaata aggggaatat
     3241 tttcaagcat tgtgatcttg tgaccacagt gaggacaatt agatcttggc gtaataagat
     3301 taaatacggg taataattta gcgggttccc ccttcaattc cgcacattgc tgatgccaag
     3361 caagctccat cattttgggt agtcgataaa taacgacatt taaaaaacta ccaactgtaa
     3421 ttcctagaac tccacaaatt gttacaaata attctggcga tgcttgaagg gaatttataa
     3481 atgccatgat attaataggt caataaaaca aacaaattaa gcttagccaa cgacttgccc
     3541 cattttgaaa ataggtaaat acattgccac aaccattccc ccaattaatg tacctaaaac
     3601 aaccatgatc ataggctcca ttaagcttga taaagcttcc actgcattat ctacctcagc
     3661 ctcaaagaaa tcagcaacct tagataacat cgcatccaaa gaaccagcct cttcaccaat
     3721 cgcaaccatc tgaagtacca tactcgagaa tacctgagtt tctgtcatgg cagcaactaa
     3781 gctactccct gtacttactt cactttgaat atgtttcgtg gcctcataat agacgtaatt
     3841 accagctgcc cctgcaactg aatcaagcgc ttctaccagc ggaacccctg cagcgaacat
     3901 tgtcgataac gttcgtgacc agcgtgcaac agttgctttc ctaatcacat ctccaaaaat
     3961 agggagcctt agcaataact tatccataac acgttgcatt gcaatggatc gtttccaggc
     4021 ataaaagaag ctatataaac tggcaccgat cccaccaaaa atagcccacc aatagtcaac
     4081 aaagaaatca gaaattgtca taaccattaa tgtaggcccc ggcaaatctg ctccaaaccc
     4141 ttgaaatagt tctttgaatg caggaataac aaaaatcata atcactgctg taatcacgaa
     4201 cgcaacaaca ataattgaga ttggataaaa taatgcagac ttaattttac cttttatagc
     4261 ctgaatcttt tctttatacg tagctaaacg atccagcaaa ctatccaata tacccgcagc
     4321 ttcaccagca gcaactaaat tacagaataa agaatcgaaa taaaggggat attttcggaa
     4381 cgcattggct aagctacttc ctgtttcaac atcaagctta atatccatta atagtttgcc
     4441 cactgccgga ttactatgcc ccttaccaac aatatcaaaa gcttgcaaca aaggaacccc
     4501 tgatttcatc atcgtagcta gttgccgagt aaacaacgtt atatccttat ctgaaattga
     4561 tccactggat tttgttttag caactttagt aacacgaata ccttggcgcc gtagcgtgga
     4621 atttacaacc tcaacacctg ctgccctcat ttctccctta attaatttcc cagttttatc
     4681 cttcccttcc caagtaaaac taatttcttt gacttttttg tcattacttg cggcaacagt
     4741 tgccataatt actccttatc atacccaaat gttagtgatt caaatcaacc ttgatcaaaa
     4801 caaagctttg atagattaaa cttgtaatct ataaaattac ggcaaatcct gagaatgagc
     4861 ttagatcaaa caaataaggt ttaaggtagc tttcgttaaa ataaacagaa aatcaatata
     4921 agtaatcagt attttacagt taactacgca ctgctctatc attcataatc attttcctgc
     4981 atgatgcgta tccattatct ctaaataata attcaatgat gagtgaccca acatttattc
     5041 aattaactgc aagcatctta tttgcacttg cagtaattca cactttttca accaaatatt
     5101 ttgagcattt ggctcatatt cagcctcaac acgctggaat atggcattta ctcggagaag
     5161 ttgaagttgt ttttggtttc tgggcaatga ttctaatact ctttatgttt gctgtcggtg
     5221 gaaaaaccga agctgtgggt tacttaaatt cacgtgattt cactgaaccc atgtttgtgt
     5281 ttgtaatcat ggtcattgca ggcacaaaac ctatccttag tttcacagcc tttatagtgc
     5341 aacggactgc aaatcttcta ccaatcaatc gtgctatggc tatgtatttc ctggtactcg
     5401 cccttgttcc cttaatgggc tcttttatca cagaaccagc agcaatgacg ctagcagcgc
     5461 ttatgctacg cgacacatta tttggccaaa ccatatcaaa taaacttaaa tatgctacag
     5521 tcggtgtact gttcgttaat atctctattg gaggaacgtt aacacctttt gcagctcccc
     5581 ctgtattaat ggttgccgct aaatggaatt gggatttctg gtttatgatt acaacttttg
     5641 gctggaaatc agcgattgcg gttatgataa acgctgcact tgttacatta ctttttagca
     5701 aagagctagt aagaacaaag cagcaggata cagacactac cactacaaag atgcctgctt
     5761 taatgattgc tgctcatatc acttttctaa cactcgttgt attattcgct cattatccag
     5821 tagtattcat ggggctattc ctattcttcc tcggatttgc aacggcatat caacgctacc
     5881 aaaatccact cattttacgc gaagcgctat tagtggcttt tttcttagga ggactagtcg
     5941 ttcttggtgg tcagcaacaa tggtggctac aaccattatt aatgggcatg gatgacactg
     6001 cagtattttt tggcgcaact gccctaaccg caattacaga taatgccgct ttgacttatt
     6061 taggttcatt agttgaaggt ttgagtgaag aatttaaaat tgctttagtt gcaggggcag
     6121 ttactggtgg aggcttaacc gtaattgcca atgcccctaa cccggcagga tttgcaatct
     6181 taaaaagtaa gttcgatgag caggcgattc atccattagg tttactaatt gctgcattac
     6241 ttccaacaat gattgccatt atcgcttttc gcatactcta ggctatcaac aataggactg
     6301 aataaaataa attcagccct atccgattta tacagaatta tgctttctta gtttgagcta
     6361 ccgcttctac agctggtgcg gttactccct caaaggagtc cgctaaatcc cttttaggcg
     6421 gtgtgtcaaa ccaacgataa atcatcggca tcacaatcat tgtcatgcaa gttgctgtcg
     6481 tcaatccaaa tattacaacg acagctaggg gtttttggat ttcagaacct aaccctgtcg
     6541 cggtaaggaa tggtgctaat cctagcaccg tagtagcagc tgtcatcata acgggtcgga
     6601 aacgttggga acaggccttc ctaaccgact cttcaatact caagcctttt tcacgtaatt
     6661 ccttcacaaa agaaatgagc acaaccccat ttaaaataga agtaccccac actgcaataa
     6721 atcccactga tgccggcact gacaaatact ctctaaaaac aaataaccca acgactccac
     6781 caaccgatgc aaagggtagt actaaataaa tcagacatgc cagcttaact gaattaaaca
     6841 acataaataa catgaagaaa atagcagcga gcgtaattgg aacgatgatg ctaagtttag
     6901 ccatagcacg ctccatattc tcaaattgcc ccccccaaac aaaataataa ccttgaggta
     6961 atttaatttc cttggcagca cgttcctgca gctctgcaac gaaaccaccc aaatcacgac
     7021 catgaacatt tacaccaaca acaacacgac gacgcgcaaa ttctcgtgaa acaattgcag
     7081 gcccatcttt gacatgaata tcagcgacag cacttaatgg aactagcaca ccgcccgtcg
     7141 ccacttcacc acctatgtta ttattagctg gtcgcaatag aatttgttta attgcttcta
     7201 catcatgacg gaatccctct gggaatcgca gtagcagatt aaatcgacgc tcaccttcaa
     7261 acacctgtgt aacaaattta ccgccaatgg ctgttgaaat gatttcattg acatcggaaa
     7321 cattcagtcc gtgccgtgca attgcacgac gatcaatatc aatggtcaac tcttgttgac
     7381 cggataagcg ttcgattcgt atatctcttg caccaggtgt tgatttaaca atccgcgcca
     7441 tttgctcaga aagtttgcgc aattgttcca aatcatcgcc aaagatttta attgccactt
     7501 gagatctcac ccctgtcaac atttcatcta cccgttgtgc aatcggttga gaaagtacaa
     7561 tatttacccc gggtaatcca tccaatgctt tacggatatc ctcttcaatt tcttgctgag
     7621 ttcgaccaga gccttttaaa tctaaatcgg caattggatc ggactcgttc tgggctgccg
     7681 gatcagcagg tgtatcacca cgacctaatt tagaaacaac actcttaaca cctggaacgg
     7741 ctgcaatcag cttcattgct tcagtttcga tcgcaatcgc ttcctctaaa gaaatagaag
     7801 gtgcgcgaat gattaccggc gttattgccc cctccttcat aatcgggata aaagatttcc
     7861 ccaaaaaagg aaaaatagca aaagcaagca tgagtgagct aatagcaatc atgatggttt
     7921 ttttctgatt acgcattgca agatcaaaaa cactcaagta tgcgcttttc atcaccgcaa
     7981 taattttagt atcttgttca cctcctccct ttattaagta atcacacagc accggcgata
     8041 ataataatga tacgagtagt gcgataaaca aagaaattga aattgtaatt gcaagcggac
     8101 taaacgtttt cccttccatt ccttccagtg tcataagtgg aagaaacact agcaccgtta
     8161 cgataagtcc aaaaatcacg ggcattccaa cctctgccac agcgtcaaca ataacattaa
     8221 atttagattt acccgtatct ttggcttctc ctaagcgttg gtagatattc tccacaacaa
     8281 ctaccgtagg atccaccatc at
//
LOCUS       DAOQLJ010000104         8290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3464_length_8290_cov_6.602550, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000104 DAOQLJ010000000
VERSION     DAOQLJ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3464_length_8290_cov_6.602550"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(338..472)
                     /gene="rpmH"
                     /locus_tag="PK522_08020"
     CDS             complement(338..472)
                     /gene="rpmH"
                     /locus_tag="PK522_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNC41716.1"
                     /translation="MKRTYQPSVIRRKRTHGFLVRMRTRSGAAVIRARRAKGRARLSV
                     "
     gene            732..1199
                     /locus_tag="PK522_08025"
     CDS             732..1199
                     /locus_tag="PK522_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HNC41717.1"
                     /translation="MMHTNMTIDQRILDRRSTIPFLCPYQLGLKQGKRVLKRRSGSGA
                     AYVDTYSTYIVLCTILTIILSALDALLTLNILARGGVELNNFMAVLIDDSTEKFVSIK
                     LALTSLAMIFLLIHHHVELTQSLKVKHLLILILVGYSTLICYEAGILFFASKY"
     gene            complement(1225..2577)
                     /locus_tag="PK522_08030"
     CDS             complement(1225..2577)
                     /locus_tag="PK522_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HNC41718.1"
                     /translation="MLLFSYKQIRLLMLLAVIGGHVTIANAIGLLDAYESALMHDPTF
                     RAAIHENEAGQQAIAIGRAGLLPTLSITHLQSISRGTQTNPNIAGISEKSNLSFNSEV
                     TTLTLRQPLLNMEAVTNYDQSHARANSSQAKLIGQSHLLIVRLVEAYVKTLLAQDRLY
                     LAKAQDKALEELKIVNQNMLKRGEGTHTDVLETQSRHALAQAQVIEAQDEFDTAKLAL
                     SAMIGQDVAALDALSSHFKVQPLYPADFESWQEMALVGNAELLTQQHMVTSSKLEIQK
                     SRAGHAPRVDLVASLNRNNAASFLFPDRDATFGTIGVEVNLPLYAGGRVIATTDQAQA
                     NHARAGAEFDAIRDRVLVELRKQFQLAQSSIKRIDSLELAVDSALLLVQATDKSIKGG
                     VRINLDLLDAQRQLFLAQVNLAEAKYNYLLAYLRLRLASGTIALDDLEKVAAYFVAKN
                     "
     gene            complement(2580..3923)
                     /locus_tag="PK522_08035"
     CDS             complement(2580..3923)
                     /locus_tag="PK522_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HNC41719.1"
                     /translation="MNSVNQNNSINEGVDQATEVKTDASSHTRLGWWIVLAGVGGFLL
                     WATLAPLDKGVPLSGSVMVATNRKAVQHQTGGIVDDILVKEGDTVKAGQVLVKMNDIQ
                     VKAQAEITRGQLITARAVEARLIAERDKKSEIDFPPFLLTALDDPRAMSSISLQKQLF
                     FSRRSVLKHEIEALDESIAGMNLQVRGLEDARKSKEEQRHFLKEQLINMRELAQEGYV
                     PRNRVLELERLHAQLNSDLSTDIGNIGRLRRQISEFNLKRLQRLEEYQREVRQQLTDV
                     QREAESLNSRLLAQDFELANVLVKAPVDGVVVGINVFTRGGVIGPGFRIMDIVPSEDM
                     LVIEGQVPVHLIDKVHAGLDVDFIFTAFNQNKIPRVPGVVTHVSADRLTEERTGIPYY
                     NLKAKVVPEGMKLLTDFQIRAGMPVEIFVKTGERPLMSYLLKPFLDRLHKSLVED"
     gene            complement(3938..5722)
                     /locus_tag="PK522_08040"
     CDS             complement(3938..5722)
                     /locus_tag="PK522_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HNC41720.1"
                     /translation="MKAQKLPHSELAEILISFKRAFRTVGVFSAVINLLLLMPALYML
                     QLYDSVLTSRNEMTLLMLTLIMLGAYIFMGTLEFIRSFVLIRIGAQFDLKLNKRVYDA
                     AFEQSLRIGDSNAGQALQDLTNLRQFLTGNALFAFFDAPWFPIYILVIFLFNVWLGVF
                     ALFGTAVLIVLAYINEKISHKPLAEANAMAVASSALATNNLRNAEVIEAMGMLPNIQA
                     RWYKIHSRFLNLQAEASEKSGVVTAISKATSVALQSMMLGLGALLVLEDSISPGMMIA
                     GSILLGKAIGPVQLLISVWRQFGNTRSAYERLTKLLEENPARKPGMALPKPKGIIAVE
                     NVIAAPPGSKVAVIKGLTFSIAAGEVLGIIGPSGSGKSTLARLLVGVWPAASGKVRLD
                     GADVYQWNKDELGPHIGYLPQDIELFSGTVSENIARFGEVDAEQVILAAKRAGVHEMI
                     LNMPKGYDTPLGDGGGGLSGGQKQRIGLARSMYGDPSLIVLDEPNSNLDDIGEQALLA
                     AIVDLHKRGKTIILITHRTSIITATSKLLFLRDGVIQMFGPSSQVLAELAKQQQQAQQ
                     AMQAKRQAMDQNVQSIALTPGNQPEGKA"
     gene            complement(5873..6766)
                     /locus_tag="PK522_08045"
     CDS             complement(5873..6766)
                     /locus_tag="PK522_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC41721.1"
                     /translation="MVNQSIGVFGATSLVGKRLLPLLIQNGWEVTAFSRQKIDSEFIP
                     TTSAIKWCSLESLRENQLNFNLDQKIPYWFYLAPIWTLPAYFDFLSKQMVKKIIVLSS
                     TSIFTKVTSSDTSEIKIAKKLEEGEANLRNWAERHEINWIILRPTLIYGYGQDRNVCE
                     IARFIQRFHFFPLLGNANGLRQPIHADDVAAACYAAFLSQQVTNRVYNISGNELIAYR
                     DMVERIFVALDKSPILLKIPYWFFQLAIKLLKPFPRYSKWSITMVERMGRDLVFDHSE
                     ASRDFGFMPRAFKLGKEDIPL"
     gene            complement(6759..7673)
                     /locus_tag="PK522_08050"
     CDS             complement(6759..7673)
                     /locus_tag="PK522_08050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC41722.1"
                     /translation="MNLPSPVSLVIVNHNAGLLLTDCIRSAQSQVQEIIVIDNASTDT
                     SLAEIEQRFSSKEKQLLTIKRLDQNIGFAAACNIGIQETRSPFVLFLNPDCMLPVNAI
                     DRLMQVLQSDSQIGMVGGRLLNADGSEQGGGRRAIPTPWRALVRATGLYRLERFWPNL
                     FFDFHLHKQPLPLNPIDVEAISGALMLVRRDAIDEVGVWDEGYFLHCEDLDWCMRFRH
                     KGWRIVFVPDVQVLHHKGACSQARPLFVAWHKHKGMLRFYQKFFRDQYPSVLMWLVST
                     GVWFRLGAVVFFHYLSQLGRFCLRQRRG"
     gene            7819..>8290
                     /locus_tag="PK522_08055"
     CDS             7819..>8290
                     /locus_tag="PK522_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HNC41723.1"
                     /translation="MLQNITSKHRQEKFIREVKTNGLLTAIKKAYILLRQLQKPLSNQ
                     LPNIDTKSYFAQLFQQAFKSESYIPFRINQIQSLRSDIKLIAFYLPQFHPIKENDEWW
                     GKGFTEWTNVTKAVPQFLGHYQPHLPDVLGFYDLRLKEVQQQQITLAKQYGIYGF"
BASE COUNT         2513 a         1923 c         1499 g         2355 t
ORIGIN      
        1 agatttcgca atctaaaaat ataatcgtta ttccctaact taggcctgtg caaacgaaaa
       61 acctctctca gcactcgctt taatttattt cgtctcacgg cagtccgcgc tacttttctc
      121 gcaacaacca agccaagtct gggaaaattg cctttattcg gcttcaagta tagctgcaat
      181 agttgggaac ttacagattg tttaaactga ataaccgatt gaaactctct cgcccgatga
      241 agcctaaatt ttttgggaaa tgaaaagatt ctctttcacc caaccatttg ataaaggact
      301 cttcttttct actttatact gactttaagc acgaggccta gacacttaaa cgtgcacgac
      361 cttttgctcg acgtgctcga ataacggcag cgccactacg ggttctcata cgtacaagga
      421 aaccatgcgt acgttttctt ctgataactg agggttgata agtgcgtttc atcttaaact
      481 ttctttttag ttgatcaaat aaatataaac ttcatttctg taacagtata ttacaacctt
      541 ttaaaggtag tgatgtcaac cttaatttca cccaattaat tacagcaagc tttatcctaa
      601 gacgacacga tcaattcact tagcttgtac atgagtacag ttgttcctga acccacagat
      661 aagaataatc atgtttcgtt tcttacccag tttagcagga ctaattgtta attattgaaa
      721 ggttataagc tatgatgcat acaaatatga ctattgatca aagaatttta gaccgccgtt
      781 ctacaatacc ctttctttgc ccctatcagt taggtcttaa gcaaggaaaa cgcgttctta
      841 agcgacgctc aggcagtggc gctgcttatg ttgataccta ttccacttac atcgtgcttt
      901 gcaccatttt aacaatcatc ctcagcgcat tagatgcttt acttacgctt aatattctag
      961 ccagaggggg cgtagagctt aataacttca tggcggtact tattgatgat agtactgaga
     1021 aattcgtaag catcaagcta gcacttacat cgcttgcgat gatcttcctc ttgattcatc
     1081 atcatgtcga attgacacaa tcattaaaag ttaagcattt gttgatacta atcctagtcg
     1141 gctatagtac gctcatttgt tacgaagcag gaatactatt ttttgcaagt aaatattaat
     1201 cgaaattata agcactaaag taggctagtt ttttgcaaca aagtaggcag caactttctc
     1261 caagtcatct aaagcgatag ttcctgacgc caggcgaagt cgtaagtagg ccaaaaggta
     1321 gttatatttt gcttctgcaa gattaacttg cgctaaaaat agctgccttt gtgcgtccag
     1381 caaatcaaga ttgatgcgca cgcctccttt aatgctttta tccgtcgctt gtacaagcaa
     1441 taacgctgaa tctacggcta attcgagaga atcaattcgc ttaatactgc tttgtgctaa
     1501 ttgaaattgt ttacgtaatt cgaccagcac tcgatctcgg atcgcatcaa actccgcgcc
     1561 ggctcttgca tgattcgctt gggcttgatc cgttgtcgca ataacacgtc cgcccgcata
     1621 aagtggcaaa ttaacttcca cgccaatggt tccaaacgtt gcatcacgat cgggaaaaag
     1681 aaaagaagca gcattgttac ggttcaagct tgctacaaga tccacacggg gggcatgccc
     1741 agctcgactt ttttgtattt ctagcttact cgaagtaacc atatgttgtt gtgttaaaag
     1801 ctcagcatta cctaccagtg ccatttcttg ccagctttca aaatcagcgg gatacaatgg
     1861 ctgcacttta aaatggctgg ataaagcgtc caaagcagcg acatcttgcc ctatcattgc
     1921 tgataatgcc aatttagctg tatcaaactc atcttgagct tcgataactt gtgcttgcgc
     1981 taaagcatgt cgcgattgtg tttctagcac atcggtatgg gtcccttcac cgcgcttcaa
     2041 catattttga ttaacaattt ttaattcttc tagcgcttta tcttgcgcct ttgctaaata
     2101 taagcgatct tgcgcgagca atgttttcac ataagcttct accaaccgga cgatgagcaa
     2161 gtgactttga ccaatcagtt tagcttggct agagtttgct cgggcatggc tttgatcata
     2221 attggtaaca gcctccatgt tcagcaaggg ttgtcgcaat gttaatgttg tgacttcact
     2281 attaaaactt agattagatt tttcactaat ccctgcaata ttaggattcg tttgcgtgcc
     2341 tcgacttata ctctgcagat gagttatcga taaagtgggt aatagtcctg cccgaccgat
     2401 tgcaattgct tgctgtccgg cttcattttc atgaatagcc gcacgaaaag ttggatcatg
     2461 cattaaggct gactcatacg catccaataa accaattgcg tttgcaatgg taacgtgccc
     2521 ccctatcaca gcaagcagca taagtaaccg aatttgttta taagaaaaca acagcacgat
     2581 taatcttcca ccaatgattt atggagtcgg tccaaaaaag gctttagcaa atagctcatt
     2641 aacggtcgct ctcccgtttt cacaaaaatc tcaactggca tacctgctcg aatttgaaaa
     2701 tcagtcagca acttcatccc ttcaggaaca acttttgcct ttaaattata atagggaatt
     2761 ccggtacgct cttcagtcaa ccgatcagcg gatacatggg ttacgactcc aggtactcgt
     2821 ggaattttgt tttgattaaa cgcagtaaaa atgaaatcga catcaagtcc tgcatgcact
     2881 ttatcgatta aatggacggg aacttgccct tcgatcacga gcatatcctc agaaggtaca
     2941 atatccatga tgcgaaaccc aggaccaata acacccccac gagtgaacac attaataccg
     3001 actacaaccc catcaacagg agcttttact aacacattag ctaactcaaa atcctgtgca
     3061 agtaaccgac tattcaatga ttccgcttcg cgttgcacat cggtcaattg ctgacgaact
     3121 tcccgttgat actcttctag gcgctgtagg cgctttaaat tgaattctga aatctgtcga
     3181 cggagtctcc caatgtttcc gatgtctgtt gaaagatcac tattcaattg cgcatgaaga
     3241 cgctcaagct ccaaaacccg attacgggga acataacctt cttgcgccaa ctcacgcata
     3301 ttaattagtt gttccttcaa aaagtgacgt tgctcttcct tacttttacg agcatcttcc
     3361 aaacctctaa cctgtagatt cattccagca atactttcat ctaatgcttc tatttcatgc
     3421 tttaacacag agcgcctaga aaaaaataat tgcttttgaa gcgaaatact actcatggca
     3481 cgaggatcat caagcgcagt taataaaaac ggcggaaaat caatttcaga ttttttatcc
     3541 cgttcagcaa tcaaacgggc ttcgacagcc cgagcagtaa taagttgacc tcttgtaatt
     3601 tcagcctgtg ccttgacttg gatatcattc attttcacca aaacttgacc ggctttcact
     3661 gtatcacctt ccttaacgag gatatcatca acgatacccc ctgtttggtg ctgtacggct
     3721 ttccgattgg ttgcaaccat aacagatcca gaaagtggca cgcctttatc aagtggcgca
     3781 agggttgccc acaatagaaa accgcccacc ccagctaaaa caatccacca tccgagccga
     3841 gtatgagatg acgcatctgt ttttacttcg gtagcctgat ctacgccttc attgattgaa
     3901 ttgttttgat taactgaatt catagtgaaa ttaaaaacta cgctttccct tctggttggt
     3961 tacccggtgt taatgcgata gattgcacat tttggtccat tgcttgacgc ttggcttgca
     4021 ttgcttgctg tgcttgttgc tgctgcttcg ctaattcagc aagaacctgg ctgctaggcc
     4081 caaacatctg tatcacacca tctcgcagga ataatagttt actcgtcgcc gtaattatac
     4141 tggtacgatg tgtaatcaaa ataatcgttt tgccacgttt atgtaaatca acaattgctg
     4201 ccaataacgc ctgctcacca atatcatcga gattagaatt aggttcatca agcacgatca
     4261 gcgaaggatc gccatacatc gaacgcgcta atccaatacg ctgcttttga ccgcctgata
     4321 accccccccc cccatctccc aagggagtat cataaccctt gggcatattc aaaatcattt
     4381 cgtgaacacc tgcgcgcttt gccgccaata tgacctgctc agcatcgacc tcaccaaagc
     4441 gagcaatatt ctcactcacg gtcccagaaa ataattcgat atcttgaggc aaataaccga
     4501 tatgaggccc tagctcatct ttattccatt gataaacatc cgcaccatct aaccgtactt
     4561 tcccagatgc agcaggccat acaccaacga gtaaccgtgc cagggtagat ttcccagatc
     4621 cactgggccc aataataccc agaacttcac ccgccgcaat cgaaaaggtc aatcccttga
     4681 tgacagccac ttttgaaccg ggcggtgcag cgattacatt ctcaactgct attatccctt
     4741 tcggtttagg caatgccata cctggcttac gcgctgggtt ttcttccaat aactttgtca
     4801 atctttcata ggcactgcgg gtattaccaa attgtcgcca aacactaatc aataattgta
     4861 ccggccctat cgctttacca agcaaaatag agcccgcaat catcataccg ggtgaaatac
     4921 tgtcctcgag tacgagcaac gctcccaagc ctagcatcat tgattgcaaa gcaactgaag
     4981 ttgccttact aatggcagta acgacgcctg acttctcgct tgcttctgct tgtaaattaa
     5041 gaaatcggct atgaatttta taccagcgcg cctggatgtt tggaagcatc cccattgctt
     5101 cgattacttc tgcatttcgg agattattgg ttgcaagtgc tgaagaagct acagccatgg
     5161 cattcgcttc agcaagcggt ttgtgcgata ttttttcatt aatataagca agcactataa
     5221 gaacggccgt tccaaatagt gcaaacacac caagccaaac gttaaataga aaaataacca
     5281 atatataaat gggaaaccag ggcgcatcaa aaaaagcaaa caatgcattt cctgttaaaa
     5341 attgacgtaa attggtcaaa tcttgtaatg cttggcctgc attgctatcg ccaattcgca
     5401 gactttgttc aaaggcagca tcatagacac gcttattcaa cttcaaatcg aattgggccc
     5461 cgattcgaat cagcacaaaa cttcgaataa actctaacgt tcccatgaag atataagccc
     5521 ctaacataat tagggtcagc atcaagaggg tcatttcatt acggctagtc aaaacgctgt
     5581 cataaagctg taacatatac aacgcaggca ttaacaaaag taaatttatg accgcactaa
     5641 acacaccaac cgtgcgaaaa gcccgtttaa aactaattaa aatttctgca agttcactat
     5701 gaggtaactt ctgtgctttc atttaaaaaa ttagaaaaat aagcgatttg tcttacagat
     5761 gatctcttgg taatagtgcc aatcaagcca agtcaagaat gtcggccatt aaaccaaaaa
     5821 ataccttaat gcgaatgtgt aatttctcac agattcttga tgcgcatgat ggctaaagtg
     5881 ggatatcttc tttacctaac ttaaacgcgc gcggcataaa accaaaatct cgcgaggctt
     5941 ccgaatgatc aaatacaaga tctcttccca ttcgctcaac cattgtgata gaccatttgc
     6001 tgtatcgagg aaatggtttt aacagcttta ttgctaattg aaaaaaccag tacgggattt
     6061 tgagcaagat tggcgattta tctaatgcga caaatatccg ttcgaccatg tcccgatagg
     6121 caatcaattc attcccgcta atattatata ctcgattagt tacttgctgg gatagaaatg
     6181 cagcataaca tgccgcagca acatcatctg catggatagg ttgtcttaag ccatttgcat
     6241 ttcctaataa ggggaaaaaa tgaaaccgtt gaatgaagcg agcgatttca caaacatttc
     6301 gatcttggcc ataaccataa ataagggtag ggcgtaaaat gatccaatta atctcgtgcc
     6361 tctcagccca atttcgtaga tttgcttcac cttcctcaag ttttttggcg atttttattt
     6421 cgcttgtatc cgaggaagtc actttggtaa aaatacttgt agaagataaa acaattattt
     6481 tttttaccat ttgtttagat aaaaaatcga aataagcagg caaagtccag atcggcgcca
     6541 aataaaacca atagggtatc ttctgatcta aattaaaatt aagctgattc tcacgcaatg
     6601 attccaatga gcaccattta attgcagaag tggtcgggat aaattcagaa tcaatttttt
     6661 gcctcgaaaa tgccgtcact tcccaaccat tttgtattag tagtggtaat aagcgctttc
     6721 caaccagact agttgctcca aaaacaccaa tactttgatt aaccacgtcg ttgtctcaag
     6781 caaaaacgcc ctagctggga aagataatga aaaaaaacta ctgcccccaa acgaaaccaa
     6841 acacctgtag agacaagcca cattaacacg gaagggtact gatcacgaaa aaatttctgg
     6901 taaaaccgca acataccttt atgcttatgc caagcaacga agagcggcct agcttggcta
     6961 catgcgcctt tatgatgcaa tacctgcaca tctggaacaa agacaattcg ccatcctttg
     7021 tgcctaaacc gcatgcacca atcgagatcc tcgcaatgaa gaaaataacc ttcatcccaa
     7081 acacccactt catctattgc gtcacggcgc accaacatca atgcaccaga aatagcctct
     7141 acatcgatag gatttagggg taaaggctgt ttatgcaaat gaaaatcaaa aaaaaggtta
     7201 ggccaaaacc tctccaagcg atataaaccg gtcgctcgca ccagtgcacg ccaaggtgtt
     7261 ggaatggctc gccgaccacc gccttgctca cttccatccg cattaaggag tcggcctcca
     7321 accattccga tttggctatc ggattgcagc acttgcatga gacgatcaat cgcattgact
     7381 ggaagcatac aatcaggatt taagaataaa acaaaagggg atcgggtctc ttgaattcca
     7441 atattacagg cagcagcaaa tccgatattt tgatctaagc gtttaatcgt gagcagttgt
     7501 ttttctttac ttgaaaacct ttgctcgatt tcagccaaac tcgtatcagt ggaagcatta
     7561 tcgataacaa taatttcctg aacttgactt tgagctgagc gaatgcaatc agttagtaac
     7621 aatcctgcat tatgatttac aatcacaagc gataccggtg atggaagatt catttattca
     7681 ttaataagat gctgcatatt caatcaatct acatccaagc caatcgcaag tagtataaat
     7741 ctaatctcca ttatatagct caaccgttta tttataagca aattcgtaaa accacagctt
     7801 caatcaatgc tcaaattatt gcttcaaaat attactagca aacatcgaca agaaaaattc
     7861 atacgggaag tcaaaaccaa tggtttgtta accgcaatta aaaaagcata tatcttgttg
     7921 cggcagttac aaaaaccctt atccaatcaa ctccctaata ttgatacgaa atcttatttt
     7981 gctcagctat tccagcaagc ctttaaatca gaaagctata ttccttttag aattaatcag
     8041 atacagtctc tgcgttctga tattaaatta atcgcatttt accttccaca atttcatcca
     8101 atcaaagaaa atgatgaatg gtggggaaaa ggttttaccg aatggacgaa tgtaaccaaa
     8161 gcggttccac aatttctcgg gcattatcaa ccgcacttac ccgatgtact gggtttctat
     8221 gatttacgcc taaaagaagt acagcagcaa caaattacat tagccaagca gtatggaatt
     8281 tatggattct
//
LOCUS       DAOQLJ010000105         8053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3602_length_8053_cov_6.239560, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000105 DAOQLJ010000000
VERSION     DAOQLJ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8053
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3602_length_8053_cov_6.239560"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..579
                     /locus_tag="PK522_08060"
     CDS             268..579
                     /locus_tag="PK522_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNC41724.1"
                     /translation="MTSVIYRITYPNNKIYIGQDRTNSINYFGSASSELISQDFTNEQ
                     RQSFTITRDILWSSECASQSEVTHVEYELIERYHANNPAVGYNQFPPFKKTNYEVKKE
                     S"
     gene            complement(587..1213)
                     /locus_tag="PK522_08065"
     CDS             complement(587..1213)
                     /locus_tag="PK522_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HNC41725.1"
                     /translation="MENEQPPQQSQHNTSDLLQLVVVQNVNDKLSIGEIKNMLHERGF
                     GVLLVIAAIPICLPIPAPPGFTTVFSLPLIIFSLQMICGMRSPWLPRWLTDKKISRKG
                     LEKLVQKAAPWLRKIESYLHPRITYISVSAWERIIGIFVLIFSITIALPIPMINFLPG
                     WGVMIMSLGLLSKDGLVIIGGMIVGTIGVGIALIAVILIWAGVAPAPL"
     gene            complement(1319..2836)
                     /locus_tag="PK522_08070"
     CDS             complement(1319..2836)
                     /locus_tag="PK522_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNC41726.1"
                     /translation="MSQSSTNIHKRLKSLEKHLADEHPNNPVLAKAVHSFRKLDRVAQ
                     DMGLLDHNESYATYVTWWPMIAVLGTFSAGKSTFINSYLNLNLQRTGNQAVDDKFTVI
                     CYTDQDEVKTLPGVALDADPRFPFFQISRSISEISETGPQRIDAYIQLKTCPSEKIRG
                     KILIDSPGFDADSQRTSTLRLTQHIIDLSDLVLVFFDARHPEPGAMKDTLEYLVSGTI
                     NRADANKFLYILNQMDVTAKEDNPEEVVSAWQRSLAQVGLLAGRFYRIYDPKAANPIN
                     DAQVKERFEKKREEDMADINARMQQIEVERSYRIAGMLEQTAETIETQVVSKLTDMLD
                     QWKKSVFLFDGILFTLLFIACGVILTMTESWGILQPFAENVIAQDTQSLIILGIMVSI
                     IGYIHFSIRGAVAKSLVKKLPAEFGHDHNSCKQYTQAFLKCTSAFRPMFLSKPSGWNS
                     SNQQILAEVKDEANDYIQSLNDLFTDPSGRKADAETVNEPVIDEQQNQPTQQASQ"
     gene            complement(3000..3908)
                     /locus_tag="PK522_08075"
     CDS             complement(3000..3908)
                     /locus_tag="PK522_08075"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HNC41727.1"
                     /translation="MNNAKKPLTTPWLDITQQIRQFDRNFNILAIDTIGGGCINQTYR
                     IQGDHCDYFVKLNKASFLPSFEAEAAGLTEIQETHTIRVPEPKCYGYNTANAWLVLEY
                     ISLNPVNKNGYLTLGQNLAALHHITSQQFGWFRDNTIGATLQKNNHTANWVDFWRHQR
                     LGYQLHLAKQKGYGGKLQQLGEKLLTDLDLFFPSQSIVPALLHGDLWSGNCASDQSGQ
                     PIIYDPAVYYGDRETDLAMTELFGGFPSQFYAAYQEAYPLDKGYNIRKTLYNLYHILN
                     HLNLFGGGYLRQAEQMQVKLLAELRS"
     gene            complement(3914..5296)
                     /gene="argH"
                     /locus_tag="PK522_08080"
     CDS             complement(3914..5296)
                     /gene="argH"
                     /locus_tag="PK522_08080"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNC41728.1"
                     /translation="MKKHTNTWSGRFNEPVSELVQRYTASVNFDKRLAEYDIQGSLAH
                     AQMLASVGIISDADLVAIEQGLTTILQEIRDNTFDWKIELEDVHLNIEKRLTALIGDA
                     GKKLHTARSRNDQVATDIRLYLRASIDEITSLIQAVQLALLNLAENHIDTIMPGFTHL
                     QVAQPISLGHHLMAYHEMLARDRERLTDCRKRLNRLPLGAAALAGTSYPIDRMFVAKQ
                     LAFDRVCSNSLDAVSDRDFAIEFCACAALIMMHLSRLSEEIIIWMNPLFNFIQLADRF
                     CTGSSIMPQKKNPDVPELVRGKTGRVNGHLIALLTLMKSQPLAYNKDNQEDKEPLFDA
                     IDTLTDTLRIYSEMIPGITVNHEAMRNAAMRGYATATDLADYLVKKGIPFRDAHEMVA
                     QAVRFAESKSCDLSKLTLTELQQFTPLIEEDIFSVLTLEGSIKSRNHVGGTAPDQVRQ
                     AIAQARHDLT"
     gene            5537..6193
                     /locus_tag="PK522_08085"
     CDS             5537..6193
                     /locus_tag="PK522_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41729.1"
                     /translation="MTDQYKQNDSKNTINWTNPDALLNMLSGKFAAFDKFQKMQLPSW
                     SVWVFLGILLLVIAWSQIVVSQANSRLEKGQAEIANQLELERSTLIRKAREYADAQYH
                     KEEDRFGQVLSWAVRGELLRNNLDQVSQYLNELVKIKDTERAVLIGDDGKLLVSTDKK
                     LEEAAAKEIYPEELLQQRKITIQSDVNGRKLLIVPIMGLNNRLATVVISYKNPPLNSN
                     "
     gene            complement(6254..7507)
                     /gene="murA"
                     /locus_tag="PK522_08090"
     CDS             complement(6254..7507)
                     /gene="murA"
                     /locus_tag="PK522_08090"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNC41730.1"
                     /translation="MQKLLIQGGYPLQGEITISGAKNAALPLLCASLLTTDTIELSNV
                     PELMDIVTMLSLLQQMGVATRRKDHSKIELSTKDVSNPTAPYEMVKTMRAAILVLGPL
                     VARVGEAHISLPGGCAIGLRPVDQHIKGLQAMGAEIAIEHGYIRAQASKLRGARILMD
                     IVTVTGTENLMMAATLASGLTIIENAAREPEIIDLANCLNQMGAKISGAGTDIITIEG
                     VEALHGVSYEVMPDRIETGTFLTAATATTGEILLKKARAETLDAVTSKLLEAGALIDF
                     DRDQIHLVMNKRPQSVSLRTAPHPAFPTDMQAQFMALNAIAEGTALITETIFENRFMH
                     VQELKRLNADITVEGNTAVIKGIQQLDGANVMATDLRASASLVIAGLVARDETTIDRI
                     YHLDRGYESIEKKLSSVGAQIKRVT"
     gene            7640..8029
                     /locus_tag="PK522_08095"
     CDS             7640..8029
                     /locus_tag="PK522_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HNC41731.1"
                     /translation="MAKQIIQTVDAPQAIGTYSQAVRVNGGDTIYLSGQIGLDPVTMQ
                     MVEGIDAQIERVILNLKAVAKASGGSLSDIVKLNIFLIDLANFAKVNEAMAQHFQQPY
                     PARAAIGVASLPRGALVEMDGVMVLSN"
BASE COUNT         2336 a         1590 c         1763 g         2364 t
ORIGIN      
        1 atcgatggcg ctattcggtt cagcattgcc atctgatgta aatttcaatc ttaacctaac
       61 gttaaatgcg atacctgaac ctgttacgct ggctttagtc gggttaggta tattgggaat
      121 gacagcagtg ggtcgccgtc gtagtaacac cagcaatatt agcttcgctt gatatattga
      181 acaggtaaag tcattttgat caaaatgact ttactgtggt aggtagtata ttagatagat
      241 tctaactatc tagtaggatc gagtagtatg actagcgtaa tttaccggat tacgtatcca
      301 aacaataaaa tctatatcgg acaagatcgt acaaatagca ttaattactt tgggagtgct
      361 tcaagcgagc tgatctctca ggatttcact aacgaacagc gccagagctt taccatcaca
      421 agggatattc tgtggtcttc cgagtgcgcg tctcaatctg aagtaactca tgttgaatac
      481 gaattaattg aacgatatca cgctaataac ccagcagtgg gttataacca gtttccacca
      541 tttaaaaaaa caaactatga agtgaaaaaa gagagttaaa ttgattctaa agtggcgctg
      601 gcgcaacacc cgcccaaata aggataaccg caattaatgc gatgccaacc ccgattgtgc
      661 ccacaatcat accgccgata atcacaagcc cgtccttact gagtagcccg agtgacataa
      721 tcattacacc ccatcccggt aaaaaattaa tcatgggaat gggtaaggca atcgtgatgg
      781 agaaaatgag gacaaatatc ccgatgatgc gttcccaggc agagacgctg atataagtga
      841 ttcttggatg caagtagctt tcaattttac gtaaccacgg cgctgctttt tgaactaatt
      901 tttctaatcc tttgcggcta attttcttgt cggttagcca tcgaggaagc cagggtgatc
      961 gcatgccaca aatcatttgt agcgagaaaa tgatcagtgg taatgaaaaa acggttgtaa
     1021 aacccggagg agctgggatt ggaaggcaga ttgggatggc agcaataacc agtaataccc
     1081 caaatcccct ttcatggagc atattcttaa tttcaccgat acttaattta tcgttgacgt
     1141 tttgtacaac gactaattgt agtaaatcag aggtattgtg ctgcgattgt tgtggtggtt
     1201 gttcattctc catgcagtca tccagataaa gcgcgctatt tacgtaaaaa aacaaccccc
     1261 ctaactaagg gggggcttga aatgatgatt ttgtggaacc tcaatcaatt ttctaacgtt
     1321 attgggaagc ttgttgtgtg ggttgattct gttgttcgtc aatcacgggt tcgtttacgg
     1381 tttctgcatc ggctttgcgg ccagatgggt cagtaaataa atcattgagc gattgaatat
     1441 agtcgtttgc ttcgtcttta acttccgcaa gaatttgctg attagaggaa ttccaacccg
     1501 atggtttgct cagaaacatg ggtctgaatg cagaagtaca tttcaagaac gcctgagtat
     1561 attgcttgca agagttgtga tcatgtccaa attcagcagg tagttttttg actaaacttt
     1621 ttgcgacagc acctcgaatt gaaaaatgga tataaccgat tatactgacc ataataccta
     1681 aaataatcag cgactgggta tcttgggcga tgacgttctc agcaaagggc tgtaggattc
     1741 cccagctttc ggtcatagta agaatgacgc cgcatgcaat gaataaaagc gtaaaaagaa
     1801 taccatcaaa tagaaataca ctttttttcc attgatcaag catatccgtt agcttactta
     1861 ctacttgcgt ttcaatggtt tcagctgtct gttctagcat gcctgcgata cggtaagaac
     1921 gttctacttc gatttgctgc atacgggcat tgatatcagc catgtcttct tcccgttttt
     1981 tctcaaaacg ttccttgacc tgtgcgtcat taatagggtt tgccgctttt ggatcataaa
     2041 tacgatagaa ccgacctgct aacaacccaa cctgagcgag tgatcgttgc caagcagaaa
     2101 caacttcttc aggattatct tccttagcag ttacatccat ttgattcagg atatatagga
     2161 atttattggc atctgcgcgg ttaatagtgc ccgatactaa atactccaat gtatctttca
     2221 tagcgccggg ttccggatgg cgcgcgtcaa agaaaaccag tacaagatct gataaatcga
     2281 tgatgtgttg tgtcaaacgg agagtcgaag tgcgttgact atctgcatca aatcctgggg
     2341 agtcaattaa aatttttccc cgaatttttt cagaagggca agttttaagt tggatataag
     2401 catcgatacg ttgcggccct gtttctgaaa tctcagaaat acttcgactt atctgaaaga
     2461 atgggaagcg tggatcggca tcaagtgcaa caccggggag tgttttaact tcatcttggt
     2521 cggtgtagca aataacggta aatttatcat caactgcttg attacccgtt ctttgtaaat
     2581 ttaagtttaa ataggaatta atgaaggtgg attttccagc agaaaaagtt ccaagcacag
     2641 cgatcattgg ccaccaagtt acataggtcg cataggattc attgtgatcg agtaatccca
     2701 tgtcttgagc aactcgatcc aatttgcgga agctatggac tgccttggcc aagacgggat
     2761 tgtttggatg ttcatcggct aaatgttttt caagactttt gagtcgcttg tggatattgg
     2821 ttgagctttg tgacataaaa atccttagtc ttattggtta aatagttaaa tataatatta
     2881 acgagtgaat atttttgtta acgtaatcaa gctaatgctc gtggcctatt ctagtgtgtc
     2941 tactatccct ttacaatcaa gcaacacatt taaatcggta tcttaacatt tacttcatgc
     3001 tacgaacgta gctcggctag cagttttacc tgcatttgtt ccgcttgacg tagataacct
     3061 cccccaaata aattgagatg gttcaatata tgatagaggt tgtataacgt cttgcggatg
     3121 ttataacctt tatccagcgg gtaagcttct tgatatgcag cataaaattg ggaagggaaa
     3181 ccgccaaata gttcggtcat ggcaaggtca gtttctcgat ccccataata aacggcagga
     3241 tcataaatga taggttgtcc tgattggtct gaggcacaat taccactcca taaatcaccg
     3301 tggagcaaag caggaacaat cgattgacta ggaaaaaata aatcgaggtc tgttagtaat
     3361 ttttcaccca gttgctgtaa ttttccccca tagccttttt gtttggcaag atgaagttgg
     3421 tatccaaggc gttgatggcg ccagaaatca acccagtttg cagtgtgatt atttttttga
     3481 agcgtagcac caatcgtatt atcacgaaac caaccgaatt gttgggaggt aatatgatgt
     3541 agtgcagcta agttttgacc taatgttaga taaccgttct tattgactgg gttgagactg
     3601 atatattcaa gtactaacca tgcgtttgct gtgttatatc cataacattt tggttcgggc
     3661 acacgtatcg tatgggtttc ctggatctct gtaagtcctg cagcttctgc ctcaaaggaa
     3721 gggagaaaag aggctttgtt gagtttcaca aaataatcac aatgatcgcc ctgaattcgg
     3781 taggtttgat taatgcatcc tcctccgata gtatcaatcg ccaggatgtt aaagttacga
     3841 tcaaattgac gaatttgctg agtaatatca agccaaggcg ttgttagtgg ttttttggca
     3901 ttattcatat tatctaagtt aaatcatgcc ttgcttgtgc aattgcttgc cgcacttgat
     3961 cgggagccgt gccgccaaca tggttgcgac tttttatgga tccttctagt gtcaaaactg
     4021 aaaaaatatc ttcttcaatg agtggtgtga attgctggag ttcagtaagc gtcagtttac
     4081 tgagatcgca agatttactt tctgcaaaac ggacggcttg cgcaaccatt tcatgggcat
     4141 cacgaaatgg aatgcctttc ttgactagat aatctgcgag atcagtcgcc gttgcatagc
     4201 cccgcatagc agcattacgc atggcttcat gattgacagt aatgccaggg atcatttcgc
     4261 tatagattct taacgtatct gtcaaagtat caatcgcatc gaacaagggt tctttatctt
     4321 cttgattatc tttattgtag gcgagtggtt gggacttcat gagtgtgagt aatgcaatta
     4381 aatgcccatt gacacgccca gtttttcctc gcacaagttc gggtacatca ggattttttt
     4441 tttgtggcat gatcgatgaa ccagtacaga aacggtctgc aagctgaatg aaattaaaga
     4501 gcggattcat ccaaataata atttcttcag ataatcgcga taagtgcatc atgatgagcg
     4561 ctgcgcaggc acagaattca attgcaaagt cacgatctga aacagcatct aatgaattgg
     4621 agcagacacg atcgaaggcc aattgttttg caacaaacat tcgatcgatt gggtagctcg
     4681 tgccagccaa agcggcagcg cctaacggca atcgattgag acgttttcgg caatctgtta
     4741 accgttctcg gtctcttgcc aacatttcat gataggccat taaatgatgg ccaagtgaaa
     4801 tgggttgagc cacttggagg tgagtaaaac ccggcatgat agtatcgatg tggttttcag
     4861 cgagattcaa taacgctaat tgtacagctt gtattaggct ggtaatttca tcgatggatg
     4921 cgcgaagata taatcgaata tcggttgcaa cttgatcgtt acgcgatcgt gcggtatgaa
     4981 gtttcttgcc tgcatctcca atcaaagcgg ttaagcgttt ttcgatattg agatgcacat
     5041 cttcgagttc aattttccaa tcgaatgtat tatcgcgaat ttcctgaaga atggtggtga
     5101 gcccttgttc aatcgcgacc aaatcggcat cactaatgat accgactgaa gccagcattt
     5161 gggcatgcgc tagcgatcct tggatatcgt attctgctaa gcgtttatcg aaattgacag
     5221 aagcagtgta gcgttgcacc aattcggaaa caggttcatt aaatcgtcct gaccaggtat
     5281 ttgtatgttt tttcacaaaa ataagatctc cctacattta acatcatcaa atcatcagat
     5341 ttgattttaa ttattttaga ttagcaattt ctcaaattga atcttaactc gactagcaat
     5401 ttaggttatg agaattgatg catttacaac aagcgacaaa tgattgatta aaaaatgaag
     5461 aactgtcttt tacttcaggg tggaatagtg gataattacc aaatttcata cctgtcacat
     5521 caacaaggag ccatttatga cagatcagta caagcaaaat gatagtaaaa ataccattaa
     5581 ttggactaat cctgatgcac tgttaaatat gttaagcgga aagtttgcag cttttgataa
     5641 attccaaaaa atgcagttgc cttcatggtc cgtatgggta ttcttgggta ttctactgct
     5701 tgtcatcgct tggagccaaa ttgttgtaag tcaggccaat tcaagattag agaaaggaca
     5761 agccgagata gcaaaccagc ttgagctaga gcgctcgacc ttgatcagaa aagcccgtga
     5821 gtatgctgat gctcaatatc ataaagaaga agatcgtttt ggccaggttt tatcatgggc
     5881 ggtgagaggc gaattgttgc gcaataatct cgatcaagtt agccagtatt tgaacgagtt
     5941 ggtcaaaata aaagatacgg aacgtgctgt cttgattggc gatgatggga aattattggt
     6001 ttcaaccgat aaaaaacttg aagaagcggc agcaaaagaa atttatcctg aagagttgct
     6061 gcaacagcgt aaaattacga ttcagtctga tgttaacgga cgtaaattat taatcgttcc
     6121 gatcatgggg ttgaataaca gacttgcaac ggtagtcatt agttacaaaa atcccccgct
     6181 aaacagtaac taatttactg aataaaattc agtgcccctc cgtttgccag atgagcggag
     6241 ggtacttttc ccttcaagtt acgcgtttga tctgagcccc cactgaagat agtttttttt
     6301 caatggattc atacccgcgg tctagatggt aaattcgatc aatcgttgtc tcatcgcgcg
     6361 caactagccc agcaataaca agactcgctg atgcccttaa atcggtagcc attacattag
     6421 cgccatccaa ttgctgaata cctttgatca ctgcagtatt accttccacg gtaatatctg
     6481 cattgagacg tttcaattct tgtacatgca taaatcgatt ctcaaaaatg gtttcagtaa
     6541 tcaaggctgt tccttctgca atggcattga gtgccatgaa ttgggcttgc atatcagtcg
     6601 ggaaagcagg atgaggagca gttcttaagc tgactgattg aggtcgctta ttcataacga
     6661 gatgaatttg atcgcgatcg aagtcgatga gagcaccggc ttctaacaat ttacttgtta
     6721 cagcgtctaa tgtttccgct cgcgcttttt ttagcagaat ttcaccggtg gtagcggtgg
     6781 cggctgttaa gaaagtacct gtttcaatac gatctggcat aacttcatag gaaactccat
     6841 gtaaggcttc aacaccctcg atcgtaataa tatctgtgcc tgcgccagag atttttgcac
     6901 ccatttgatt caggcaatta gcaagatcga taatttcagg ttctcgtgcc gcattttcaa
     6961 taatggtcaa acctgaagca agtgttgcgg ccatcatgag attttcagta cccgtcactg
     7021 ttacgatatc catgagaatg cgggcacctc tgagtttact tgcttgagca cggatataac
     7081 catgttcgat cgcaatttcc gcccccatcg cttgaagacc tttgatgtgt tgatccactg
     7141 ggcgtaaacc aattgcacaa ccacctggta atgaaatatg agcttcacca acccgagcga
     7201 ctaatggtcc taacacaagg attgcagcac gcatcgtttt aaccatttca tagggtgcgg
     7261 ttggattaga gacatctttt gtagataatt cgatcttaga atggtcttta cggcgagtgg
     7321 ctacgcccat ttgctgaagt aggctcagca tagtcacgat atccatcaac tcagggacat
     7381 tgcttaattc aatggtgtct gtcgtcaaca atgaagcgca taacaaaggt aatgcggcat
     7441 tctttgcacc tgaaatggta atttcaccct gcaatgggta accaccctga attaagagtt
     7501 tttgcataag cgatatttaa ggaagaaaaa agatgaggat aagcggttgt tctacagcga
     7561 cagcgaagtt atgattacaa agtacgatgt actcgcaccc atattccaaa actataaact
     7621 taacataaat ggagcaagaa tggctaaaca aatcattcag acagttgatg caccgcaggc
     7681 gatcggaact tattctcaag cggttcgagt taacggcggg gatacgattt atctatcagg
     7741 gcaaatcggg ttagatcctg taacgatgca aatggtcgaa gggatcgatg cgcaaattga
     7801 acgcgttatt ctcaatttaa aagcagttgc gaaagcaagt ggcggaagct tgagtgatat
     7861 tgtaaaactc aacatctttt tgattgattt ggctaatttt gcaaaggtaa atgaagcaat
     7921 ggctcagcac ttccagcagc cctatccagc acgagcagcg attggggtag catcgttacc
     7981 tcgcggtgcg ttggtagaaa tggatggcgt aatggttcta tcgaattaat ttatccttgc
     8041 tgagataaag tct
//
LOCUS       DAOQLJ010000106         8030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3620_length_8030_cov_6.579937, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000106 DAOQLJ010000000
VERSION     DAOQLJ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3620_length_8030_cov_6.579937"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            404..1438
                     /locus_tag="PK522_08100"
     CDS             404..1438
                     /locus_tag="PK522_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HNC41732.1"
                     /translation="MHIHLLIPNLFWSDTSLKETIKNWSLPALETILTKSQLNQQPSE
                     GIEAWLCETFGISKQQDWPIAPITLNLDDPNYSLKSSSKYWLRADPVHLRIANNHLLL
                     ADSQMFKISLTEASQITDTFNRYFSKDEISFAAFYPDRWYMGLPRIPNLQTRPLSYVS
                     GKNINAQLPMTTEKGLAHKILNEVQMLLHEHPINLAREIQGELIINSVWLWGGGILPK
                     QFCCNFDQVWSNYPITQALANLCNAPHYPLPPDTNEWLQSTNEGNHLVVIDLLQKKAQ
                     YKIINEWYDCINQLEQKWFSPLLNGIKKDQIKQLTIHTFNQDLVKQFKLTPRGLWKFW
                     SFNKAFSTFH"
     gene            1660..2913
                     /locus_tag="PK522_08105"
     CDS             1660..2913
                     /locus_tag="PK522_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC41733.1"
                     /translation="MIIQTLSIERNYAEYLTQLHNNKERLANEIKHRLWAEKTTSENK
                     TRLQIIDENFPVMLAYFNSKQQCCYHNRAYRLWFSLKSEQIDNHSPQQFLNTEFYQGI
                     KENIDNLLSGEIIHGQRIQKLISGSVCLISEQFIPHFDAKGKVIGFYTLYTPRILKEH
                     ESITENSKNNLVEIRSEKTGAEEKSSQNKNSRLSSEMNFASRVLKAINQGEFHLYFQS
                     IISTNQGPNQITFCEILIRMAEEETNLIPPGAFLPLVEKYNLMPQLDRWVVGYITKWL
                     SSHPTKSNTIMCINIAKDTLCDPQFTIFIKNQLTTYNIPAKTLCFEIEEVDAKSNLSE
                     VATFTQNIRQIGCYVSLCSFGHDRTSFNLLRNIKVDFIKIDGNLICDMLYNETDQTKV
                     SEINRLAHIMKIKTIAELVESQEIA"
     gene            complement(3010..3261)
                     /locus_tag="PK522_08110"
     CDS             complement(3010..3261)
                     /locus_tag="PK522_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HNC41734.1"
                     /translation="MISSDQIKKSIEASIPCEWIQVEGEDGRHFNAVIVSAEFVGKTM
                     IQQHQLVYRALRERMKDDVHALSMRTLTPEQWVNAQSSK"
     gene            complement(3328..4083)
                     /locus_tag="PK522_08115"
     CDS             complement(3328..4083)
                     /locus_tag="PK522_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC41735.1"
                     /translation="MTGFLTLFHKELLRFWKVSFQTILAPIVSTLLYLLIFSHILESR
                     VQIYPGVSYSQFLIPGLIIMAILQNAFANSSSSLIQSKISGNIIFVLLSPLSYFEFFG
                     AYLLASVVRGLLVGLGMFIVVSQFHEVSTQFPFWIILFVLLGSMILGVLGIIAGICSD
                     KFDQLAAFQNFIILPSTFLSGVFYSTLSLSPAWQKLSYVNPFFYIVDGFRYGFLGVSD
                     VSPYLSLAAVIACFLVVSLLTLNMLKTGYKFRH"
     gene            complement(4080..4994)
                     /locus_tag="PK522_08120"
     CDS             complement(4080..4994)
                     /locus_tag="PK522_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC41736.1"
                     /translation="MIPAIEIQRLNKSFGNLVALEDIQLSIAQGEFFALLGPNGAGKT
                     TLISIVAGLVMANSGQVKVMGHNVVTSYSKARSVLGVVPQELVFDPFFTVRETLVFQS
                     GYYGIRKNKVWIDEILHRLDLADKADTNMRALSGGMKRRVLIAQALVHKPPVIILDEP
                     TAGVDVELRQALWSFVKQLNQDGHTIVLTTHYLEEAEALCQHVAMLKKGKIIVLDSIR
                     NLIDKIRDISLQLRLACEVLPMQLIKWSSIKQNECYIFKIQDYLEISEILTILKQEEI
                     EILEMKLQEPDLEEVFIRMMSDKSFIGT"
     gene            complement(5063..5347)
                     /locus_tag="PK522_08125"
     CDS             complement(5063..5347)
                     /locus_tag="PK522_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNC41737.1"
                     /translation="MAIYLDGNKVALSGSITFNNVVELTQQGIDLMDSHCQIIDLNEV
                     TEVDSSAVSMLLEWLRIAKTRKLDLHFVHLPENLSSLLKLYGLSEIILPT"
     gene            complement(5396..6013)
                     /locus_tag="PK522_08130"
     CDS             complement(5396..6013)
                     /locus_tag="PK522_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC41738.1"
                     /translation="MKKMINTLISLMLLCVALSGWAKDTAPDLLVKNTVDEVIAIIKQ
                     DNGIKKGEKDRILDLVENKILPHFNFIRMTQLAMGKNWSKVEPQQQKELVNEFQTLLV
                     RTYSNALTNYRDEVIEVNPISPQSDAKNTTVKTVVIQGRGKEPVPIDYSMEKTDNGWK
                     VFDVTVAGVSLVTNYRSTFNSQIKKGGVNGLIESLAKKNKSLEGQ"
     gene            complement(6050..6508)
                     /gene="mlaD"
                     /locus_tag="PK522_08135"
     CDS             complement(6050..6508)
                     /gene="mlaD"
                     /locus_tag="PK522_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HNC41739.1"
                     /translation="MSRTVMDFWVGLFVMAGIGALLILGLKVGNLTSYNAGEVYNLTG
                     NFENIGGLKIRAPVKSAGVVVGRVLDIQYSLETHDAIVTMGVSSSYQFPKDTFASILT
                     SGLLGEQYIGLSPGGDAVMLADGDKIMKTNSAMVLEEMIGRFLFDKASEN"
     gene            complement(6508..7305)
                     /gene="mlaE"
                     /locus_tag="PK522_08140"
     CDS             complement(6508..7305)
                     /gene="mlaE"
                     /locus_tag="PK522_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HNC41740.1"
                     /translation="MRNRIVVSIQAIGHRTIDSIWRLGVASRFFVWVLLKSTTSFLRF
                     GLVVREIYFVGVLSLIIILVSGLFVGMVLGLQGYETLQKYGSESAVGTLVALSLVREL
                     GPVMAALLFASRAGSAITAEIGLMKATNQLAAMGMMAVDPIARVVAPRFWAGVISMPF
                     LAAMFSVMGVFGGYLVTVVFIGVDNGSFWSQMQNAVDFRYDIVNGVIKSCFFGVAVTA
                     IAVFEGFDAPPTAEGVSGATTRTVVTSSLVILGLDFVLTSFMFRGVS"
     gene            complement(7298..>8030)
                     /locus_tag="PK522_08145"
     CDS             complement(7298..>8030)
                     /locus_tag="PK522_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC41741.1"
                     /translation="AMSRGKVIAVMGGSGSGKTTLLRLIGGEIKPSGGHVKVADQIVH
                     HLSRDELFKLRRKMGMLFQFGALFTDISVFDNVAFQIREHTTLPESMVRDLVLMKLQA
                     VGLRGAHHLMPAELSGGMARRVALARSIALDPMLVMYDEPFTGLDPISLSVIGNLIRR
                     LTDALGMISIVVTHDVQESLKIVDYVYFLADGVIAAQGTPQEVKNSVAPFVHQFVHGE
                     EDGPVPFHYPAQSYQKDLALENFVA"
BASE COUNT         2713 a         1766 c         1278 g         2273 t
ORIGIN      
        1 cgatattaac ttgagaacga gctgcttgca ccagcccatt tgcttccaac ccaatctgct
       61 gaatttctag cacagtatat tgatttgcca aagtttgcgc ccaaccgact gagctcgcta
      121 acccaaacaa acccattaag ataaataatc tcagcatggt aatctttaat aaataattaa
      181 gaatttgcgt aaaattctag aattttacat gaagtatttg tgatcaagca tttaagttgc
      241 aggctaaata caaagtattt agtcaatcta gccaaggtaa agtataaact gtgataaatg
      301 aatacaagct gaacgattta ttctaaagaa gctatttcta gattctctgc ctaatctgtt
      361 agattagatg attaactcat cattattgac ttataaacct tcgatgcata tccacctttt
      421 gattcctaac ttgttttggt cagacaccag tttaaaagaa actatcaaga attggtcgct
      481 acctgcctta gaaacaattt tgacaaagag ccaattaaat caacagccat cggaaggaat
      541 cgaagcatgg ttatgcgaaa catttggtat atcaaaacag caagattggc ccatagcgcc
      601 cattacctta aacttagatg atcccaatta cagcctaaaa tcgagttcta aatattggct
      661 gcgcgctgat cctgtgcatc tcagaattgc aaataaccat ctgctcttgg cggatagtca
      721 aatgttcaaa atatctttaa cggaagcaag ccaaattacc gatacattca accgatattt
      781 ctctaaagat gaaatctctt ttgccgcttt ttatccagat cgatggtaca tgggcttacc
      841 cagaattccc aatcttcaaa caagaccgct cagctatgta agcgggaaaa atatcaatgc
      901 gcagcttcca atgacaacag aaaaagggct agcacacaaa attttaaatg aagttcaaat
      961 gctccttcac gagcatccca ttaatctagc aagagaaatt caaggtgagt taattatcaa
     1021 tagcgtttgg ttatggggag gtggaatatt acctaaacaa ttttgctgta actttgatca
     1081 ggtttggagt aattacccta taactcaagc gctagcgaat ttgtgcaatg cgccgcatta
     1141 cccgctcccg ccagatacaa acgaatggtt gcagtctact aatgaaggta accatctagt
     1201 agtaattgat cttttacaga agaaggcgca atacaaaatt attaatgaat ggtatgactg
     1261 cataaatcaa ttagaacaaa aatggttctc ccctttattg aacggaataa aaaaagatca
     1321 gattaaacaa cttacgattc atacgtttaa tcaagattta gtaaaacaat tcaagctaac
     1381 ccctaggggt ctttggaaat tttggtcttt caataaagca ttctcaacat ttcattaatt
     1441 tagatcctag ttattaattt ataaaatact cattaaataa ccgaccgatc aatgaacgaa
     1501 caactatctc ccagaattag ccacacaaag tcctatgttg tttcatctat tgctatcatt
     1561 gttttagcaa tactcatggc atcaaacttt ctgactctgg gacttgaatc aaatgtaatc
     1621 agtatcttgt ggggcaccct tgtcattgtc gtgataacaa tgattataca aacgctcagc
     1681 attgaacgaa actacgcaga atatttaacc cagttgcata ataataagga aaggcttgca
     1741 aatgaaatta agcatcgttt gtgggctgaa aaaactacat ccgaaaataa aactcggctc
     1801 caaattatcg atgaaaactt tccagtcatg cttgcttact tcaactccaa acagcagtgc
     1861 tgttatcaca acagagccta tcgattatgg tttagcttaa aatctgagca aatcgataat
     1921 cattccccac agcaatttct aaacacagag ttttatcagg ggataaaaga aaatattgat
     1981 aacttattaa gtggtgaaat cattcatggc cagcgtatac agaaacttat tagtggctca
     2041 gtttgcctta tctcagaaca attcattccc cattttgacg ctaaaggaaa agttattggc
     2101 ttttatacac tgtatacgcc tagaatacta aaagaacatg agtcaattac agaaaattct
     2161 aaaaataacc tagttgaaat tagatcagaa aaaaccggcg ccgaggaaaa atcaagtcag
     2221 aataaaaatt ctcgcctttc ctcagaaatg aattttgctt ccagagtact aaaggccatt
     2281 aatcaaggtg aatttcattt atatttccag agtattatct ctacgaatca aggtcctaat
     2341 cagattactt tttgcgaaat tttaattcga atggccgagg aagaaacaaa tttaattcca
     2401 ccaggcgcat ttttaccttt agtagaaaaa tataatttaa tgccacaact tgatcgctgg
     2461 gttgttggct acataacaaa atggctttct tcacatccca cgaagtctaa tactatcatg
     2521 tgcattaaca tagccaaaga taccctttgc gatccccaat tcactatctt tattaaaaac
     2581 caattaacaa catacaatat tcctgcaaaa acactatgtt ttgaaatcga agaagtcgat
     2641 gcaaaatcca atctttcaga agtcgctacc tttacacaaa atattcgaca aataggttgc
     2701 tatgtttcac tctgtagttt tgggcacgat cgaacttctt ttaatctgct tagaaatatt
     2761 aaagttgatt tcataaaaat tgatggaaat ctaatctgtg acatgcttta caatgaaact
     2821 gatcaaacta aagttagcga aattaatcga cttgctcaca ttatgaaaat taagactatc
     2881 gctgagcttg tagaatctca agaaatagct taaagcttcg tgaaatcggg gttgattttg
     2941 tgcaaggctt tggaattgcg caaccacaac cgctcaatgc gctcacagaa acatcagatt
     3001 agtattcatc tatttacttg attgtgcatt aacccattgc tctggtgtaa gtgttctcat
     3061 cgatagcgca tgaacatcgt ctttcatccg ctcacgcaat gcacgatata ccaattgatg
     3121 ttgctgaatc attgttttac caacaaactc tgcactgaca atcacggcat taaagtgacg
     3181 accatcttct ccttctacct gaatccactc acagggtatg gatgcttcaa tagatttttt
     3241 aatttgatcg gaagaaatca taattacctc agtagtatgt aaaattaata aaagtgcata
     3301 aaaatttaat catcgttacc agaataacta gtgcctgaat ttataacctg tcttaagcat
     3361 gttcagtgtt agcaatgaaa caaccaaaaa acaagcaatt acagcagcca aactcagata
     3421 gggcgacaca tcagaaacac ctagaaatcc atagcgaaaa ccgtcaacga tataaaaaaa
     3481 aggatttaca taagaaagct tttgccaagc aggtgaaaga gaaagagttg aatagaatac
     3541 cccagataag aatgtagaag gcaaaataat aaaattctgg aaagcagcaa gctgatcaaa
     3601 tttatctgaa caaattccag caattatccc cagaactccc agaatcatac tccccaataa
     3661 aacaaacaat ataatccaga atggaaattg tgttgatact tcatggaact gggataccac
     3721 aataaacata cccaaaccca ctagcaaacc cctcactaca gaagctaata aataagcacc
     3781 aaaaaactca aaataagata gaggcgacag cagcacaaaa atgatattgc cagaaatttt
     3841 tgactgaatt aggcttgatg atgagttggc aaaagcattc tgtaaaatag ccataattat
     3901 taacccagga atcaaaaact gagaatagga aacgcctgga taaatttgga cacgtgattc
     3961 taatatatgt gaaaaaatca acaaataaag caaagttgat acgataggcg ccaaaattgt
     4021 ttggaaactg accttccaga acctcaagag ctctttatga aataaagtca aaaaacccgt
     4081 catgtcccta taaatgattt atcgctcatc attcttataa agacttcttc taaatcaggc
     4141 tcctgaagct tcatttcgag tatttcaatt tcttcctgct ttaaaatggt aagaatttct
     4201 gaaatttcca agtaatcttg aattttaaaa atatagcatt cattttgctt tatcgatgac
     4261 catttaatta gctgcattgg taatacttca cacgctaaac gaagctgtaa cgagatatca
     4321 cggattttat caatcaaatt acgaatacta tccaaaacaa taatttttcc tttttttagc
     4381 attgctacat gttggcataa tgcttcagct tcttctaagt aatgagtcgt caacacaatt
     4441 gtatgtccat cttgattcag ctgtttaacg aaagaccata aagcttgcct aagctcaaca
     4501 tccactccag ctgtcggttc atcaagtatg attaccggag gtttatgaac cagggcttgc
     4561 gctatcaata cccttcgctt catacctcca gaaagcgctc gcatattagt gtctgcttta
     4621 tcagccagat caagtcggtg caatatttca tcaatccata ctttattttt tctgatgcca
     4681 taatatccag attggaaaac cagtgtctca cgcacagtaa aaaaaggatc aaaaactaat
     4741 tcctgaggaa caacgccaag cacacttcgg gcttttgaat agctagttac aacattgtga
     4801 cccattactt taacctggcc actattagcc atgaccaatc ctgccacaat actaatcaat
     4861 gttgttttac ccgcaccatt tggcccaagt aatgcaaaaa attccccttg agcgattgaa
     4921 agttgaatat cctccagcgc aaccaaatta ccaaatgatt tattcagtct ctgaatctcg
     4981 attgctggaa tcatctacaa acaaaagaaa caaacctact ttaattaaag ttaagcaaaa
     5041 actgtgatcg agaaatgact tattaggtgg gtaagattat ttcactcaaa ccatacaatt
     5101 tgagtaaact acttaaattt tccggcaagt gaacaaagtg aagatcaagt ttccgcgtct
     5161 ttgctatccg taaccattca agtaacatac tcacagctga cgaatcaact tctgttactt
     5221 cgttcaaatc gataatttga caatggctat ccattagatc aatcccttgc tgagttaatt
     5281 caacgacatt attaaatgta atcgaaccgc taagagcaac cttatttcca tctagataga
     5341 ttgccatgat tgattaagtt catccatgtt aattaaaact caattcgcag acttattact
     5401 gcccttctag cgatttattt ttcttcgcaa gtgattcaat taggccgtta accccccctt
     5461 tctttatctg actattaaaa gtactacgat aatttgtcac aagactgact ccagcgactg
     5521 tcacatcaaa aactttccat ccgttatctg ttttttccat actataatca attggaacag
     5581 gttctttacc tcgcccttga atcacaaccg ttttgaccgt agtattttta gcgtcagact
     5641 gaggtgaaat cggattgact tcaatcactt catcacgata attcgttagt gcgttggaat
     5701 aagttctcac tagcaaagtt tgaaattcat ttactagttc cttttgttgc tggggctcta
     5761 cttttgacca gttcttcccc atcgcaagtt gagtcatccg tataaagtta aaatgcggta
     5821 gtattttatt ttcaaccaaa tcaagaatac gatctttttc acccttcttg atcccattat
     5881 cctgcttgat gatagcaatc acttcatcaa ccgtattttt aactagtaga tcaggcgcag
     5941 tatcctttgc ccatcctgat aaagccacac atagcagcat caaagaaatc aatgtattaa
     6001 tcattttttt cataattatc tcaatcctca aaattatccg ataagtgtat taattctcag
     6061 aagctttatc aaacagaaag cgtcctatca tctcttctag caccatggct gaatttgttt
     6121 tcataatttt atcaccatcc gcgagcatta cagcatcgcc ccctggtgac aaaccaatat
     6181 actgttctcc cagtaatcca gacgtcagaa tactagcaaa ggtatccttt ggaaattgat
     6241 aagaactgct gactcccatt gtaacaattg catcatgggt ttccagacta tattgaatat
     6301 ccaatacacg tcctacaaca acaccagcac ttttaacagg tgctcgaatt tttaggccgc
     6361 cgatattttc aaaatttcct gttaaattat acacttcccc tgcattatag cttgtcaagt
     6421 tccctacttt taaccctaga atcagcaggg ctccaatacc ggccatcacg aacagcccga
     6481 cccagaaatc cattactgtt cgtgacatca actcactcct ctgaacataa atgaagttaa
     6541 tacaaaatct aatccaagaa taaccaatga agaggtcacg actgttcgag tcgtcgcgcc
     6601 tgaaacacct tcggcagtag gtggggcatc gaaaccttca aaaaccgcga tcgctgtaac
     6661 cgcaacgcca aaaaagcagc ttttgatcac accatttaca atatcatagc gaaaatcaac
     6721 cgcgttctgc atctgtgacc aaaatgatcc attatcaacc ccaataaaaa cgacggtaac
     6781 caggtatccg ccgaatactc ccattactga gaacatggca gccagaaacg gcattgatat
     6841 cacaccagcc cagaaacgtg gcgctactac ccttgcaatc ggatcaactg ccatcattcc
     6901 catcgcagct aactgatttg ttgctttcat tagtccaatt tcagcagtaa tagccgatcc
     6961 agctcggctc gcaaacaata acgccgccat cacaggacca agctctcgaa ccaaagaaag
     7021 cgcaactaat gttcccactg cagactctga accatatttc tgcagtgttt cgtagccttg
     7081 taaaccaaga accattccaa caaaaaggcc tgatacaaga ataataatca gtgaaagaac
     7141 accaacaaaa tagatttccc taacgacaag accaaatcgc aaaaaactag ttgttgattt
     7201 gagtaaaacc caaacaaaaa accgacttgc aacgcccaaa cgccaaatgc tatcaatcgt
     7261 ccggtgtcct atcgcttgga tactaaccac aatgcggtta cgcaacaaaa ttctccagcg
     7321 ccaggtcttt ttgataagat tgagcaggat aatggaatgg tacaggacca tcctcttcac
     7381 catgcacaaa ttgatgaaca aaaggtgcga ccgagttttt tacctcttgt ggagtgcctt
     7441 gcgcagcaat cacgccatcc gccaaaaaat aaacataatc tacaattttc aaagactctt
     7501 ggacatcatg tgtaaccaca attgatatca ttcctaatgc atcagttaat cgtcggatca
     7561 gattaccaat tacgctcaat gaaatcgggt ccaaaccagt aaagggttca tcatacatta
     7621 cgagcatggg atcaagtgca attgatcttg ctaaagcaac acgacgtgcc atcccaccgg
     7681 ataactctgc tggcatcaag tgatgtgcac cccttagccc tacagcctgt aatttcatca
     7741 aaaccaggtc tcgtaccatt gattctggta aggtagtatg ctcacgtatt tgaaaagcta
     7801 cgttatcaaa gacagagata tccgtgaata aggcaccaaa ctggaacagc atccccatct
     7861 ttcttcgcaa cttaaacaat tcatcccgac taaggtgatg aacgatttga tctgcaactt
     7921 taacatgacc accagaaggt ttaatttccc caccaatcaa acgtaataac gtcgttttcc
     7981 ccgagccact tccacccatc actgcaatca ctttcccacg cgacatagcc
//
LOCUS       DAOQLJ010000107         7805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3757_length_7805_cov_5.550452, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000107 DAOQLJ010000000
VERSION     DAOQLJ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7805
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3757_length_7805_cov_5.550452"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PK522_08150"
     CDS             complement(<1..451)
                     /locus_tag="PK522_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNC41742.1"
                     /translation="MFNQHHFWALICGPLLAGVVAASMHFSGWHVEACWTAAIVLLCV
                     IWWIFEPIPIPVTSLIPITFFPLTGVLAKEEVAKAFGNDLILLMLGGFMISVAMAQSG
                     THRRLALGLVNFIGGDSSRRIVFGFMCACAVLSMWISNTATALMMLPL"
     gene            complement(490..1740)
                     /locus_tag="PK522_08155"
     CDS             complement(490..1740)
                     /locus_tag="PK522_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HNC41743.1"
                     /translation="MIEKELKLFGMAASQGRWLFVLIGLSINICLGSIYAYSVFRKPL
                     ETLWDIPSSQSGYPFMIFLAVFAITMPLAGSLMAHWGPRKTATLGGLLVGIGWMAASF
                     APDITTLTLLYGVIGGAGVGIVYGCPIAVVAKWFPQKSGLAIGLTVMGFGLSALITAP
                     LMKAMIIHPEIGTLNTFFYLGVSFLFIVSLLARCLRFPPDEWSSVNVITHSSTTTANP
                     TIELSRHDMIRTRHFYALWATYTIGCLAGLMAIGIASPVGIEVAKLEPTTAALAISLF
                     AIFNGLGRPLFGTIADKLGPKQTAMLSFAMIGGASLLLSGLEEGDTTLYLFAFCVLWL
                     CLGGWLALAPTSTGIFFGKKHYAQNYGVVYTAYGVGAILGTLISGNIKDITGSYLGAF
                     PIVAGLAVLGLLIAAVGLNSVKNK"
     gene            complement(1985..4015)
                     /locus_tag="PK522_08160"
     CDS             complement(1985..4015)
                     /locus_tag="PK522_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNC41744.1"
                     /translation="MTVTLTNLDDNYIDGPGGNEIFAQLGNDTVNGADGSDTIWGEGG
                     NDSILGGNQNDTLYGGIGNDTLNGGSGVDRLEGGADNDTYIIDSNGIFLPLDTIVDSG
                     GIDTVLSSVTYTLGAAIENLTLTGAAATSGTGNVSANVITGNSANNTLNGLNGNDTLN
                     AGLGNDVLNGGDQNDTLNGDGGVLALSGGNDTLNGGNGNDTLSGGLGNDVLNGDVGDD
                     RLVGGSGNFFQPIDVDTLSGGIGNDTYVISENTDVITELSGEGTDTVESSITFNLGTS
                     GANVENLTLTGTAVINGTGNTLSNVINGNSKDNTLDGGAGNDTLKGNDGNDTLIGGPG
                     LTVVIGPFGPTFISDNDTLEGGKGNDTYIMDYTGDTITENLNEGIDLVKSSLSSYTLP
                     TNVENLTLTGVGNINGLGNSLDNVITGNAGINALSGDIGNDTLNGGGGNDVLDGGQGN
                     DTYIADGGDTISEAAGAGTDRVESSITFSLAAIANVENLTLTGAANINGTGNSLNNTL
                     VGNSGTNTLTGSGGNDVLIGGAGADILNGGNDDDTFWYDATDVSVAGNRISGGSGTDT
                     LTFMSSAVQNLNLTTLADDRITGIEQININGFNLFGPSPNNTLTLNVTDVFAISDTDT
                     LRVDGGVGDVVNSIGQGWANTGVVVIGTQNYDQYTFGTATLLVDTDIGGTIS"
     gene            4339..4770
                     /locus_tag="PK522_08165"
     CDS             4339..4770
                     /locus_tag="PK522_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HNC41745.1"
                     /translation="MKFHIFILGLFILALTACSTTRPILYPNTHYKTVGQHAAEQDIV
                     ACRQLAEEAGAQEGNASQVGNVATSTAMGAGMGAASGAVGGAISGAAGTGSLIGAASG
                     AALGLIRGLFGLARPTQPNQAYVNFVYRCLQDKGYEVTGWQ"
     gene            5382..6017
                     /locus_tag="PK522_08170"
     CDS             5382..6017
                     /locus_tag="PK522_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41746.1"
                     /translation="MLAGLMLTMALMAPELPNPIEKAVDYYQKVDSYQTIIKSYVGSK
                     SDSPDIVRYYYNKSGAVRMEVVEPFNGAVLIYNPVTELVKLWLFGYGSVPSFSLSPEN
                     KWIQSSSGQRVDRSDIGALYEDVILLQRQGKTTVVGTETVEKQAALYVIVEGNPGVAI
                     EGVSRFDLWFDVQTSLPLKVISYKADGSQIERIEMSEYQVNPAFPKGFFDH"
     gene            6023..6490
                     /locus_tag="PK522_08175"
     CDS             6023..6490
                     /locus_tag="PK522_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC41747.1"
                     /translation="MTAAFSFTTEWRFDAPHEMVCDAIIQCLDWPQWWLGAEQVEKLV
                     TGDSDGVGSVHRFTWKGKIPYHFTFTIRVTRYVPGVLLEGVAEGEVVGTGRWDFIRAN
                     DITIVRYAWTVRTNRLWMNLLAPIARPIFKWSHDQVMRQGELGLAHWLRMQSK"
     gene            complement(6555..6860)
                     /gene="ftsB"
                     /locus_tag="PK522_08180"
     CDS             complement(6555..6860)
                     /gene="ftsB"
                     /locus_tag="PK522_08180"
                     /inference="COORDINATES: protein motif:HMM:NF002058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HNC41748.1"
                     /translation="MKTVALILVTLIALVQYSLWYGKGGWLEVFELEKQLQDQHQLNQ
                     KSQNQNTILEAEIIDLKQGFDAIEELARNELGMIRKDEVFFQVQQPTEVPSVTKSKK"
     gene            complement(6860..>7805)
                     /gene="eno"
                     /locus_tag="PK522_08185"
     CDS             complement(6860..>7805)
                     /gene="eno"
                     /locus_tag="PK522_08185"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNC41749.1"
                     /translation="VSLAVAKAAAEESSLPLYRYLGGVRAISMPVPMMNLINGGAHAN
                     NNLNMQEFMVIPHGFDSFREALRCGAEIFHTLKKLISKKNMPTTVGDEGGFAPNLSGN
                     EEGLQLIVQAIDQAGYQPGTQVAIGIDCASSEFYQDGKYHLKSDGLSLTSAQFVDYLA
                     TWVDKYPIISIEDGMSEHDWDGWKLLTERLGKTVQLVGDDVFVTNSRILKEGIQRGIA
                     NSILIKVNQIGTLTETLTAVEIAKCAGYTAVISHRSGETEDTTIADIAVATNALQIKT
                     GSLSRSDRLAKYNQLLRIEEDLGDNAIYAGRSAFYQLT"
BASE COUNT         2238 a         1830 c         1628 g         2109 t
ORIGIN      
        1 caagtgggag catcatcaat gcagtcgccg tattggaaat ccacatactt aatacggcac
       61 aggcgcacat aaaaccaaaa acgatgcgtc ggctactatc gccaccgatg aaattcacca
      121 accctaatgc cagacgtcga tgcgttcccg attgcgccat cgccaccgaa atcataaagc
      181 cacccaacat cagcaaaatc agatcattcc caaaggcctt ggcgacttct tctttggcta
      241 gcacccccgt tagtgggaaa aatgtgatgg gaatcagtga agttacgggg atgggtatcg
      301 gttcaaaaat ccaccagata acacacaata aaacaatagc agccgtccaa caggcttcta
      361 catgccaacc tgagaaatgc atactcgcag caacaactcc agcaagtaaa ggaccgcaga
      421 ttaatgccca gaaatgatgt tgattaaaca tggtggacct tttattgatt acccatcttc
      481 acaactactc tatttgtttt taaccgaatt taaccctact gcagcaatca acaagccaag
      541 caccgctaat cctgcaacaa tcggaaatgc acctaaataa ctacccgtga tatctttgat
      601 attgccagag atcagtgttc ccaaaatggc acccacacca taagcggtat aaaccacgcc
      661 ataattctgg gcataatgtt tttttccaaa gaaaattcca gtggaagtgg gcgccaatgc
      721 tagccagccc cctaagcaca gccataacac acaaaaagca aataaataca aggtagtatc
      781 gccttcttcc aagcctgata acagcagtga cgcaccaccg atcatggcaa atgagagcat
      841 tgccgtttgt ttcggcccga gtttatcagc gatagtccca aacaaagggc gccctaaccc
      901 attgaatatc gcaaataatg aaatcgctaa tgctgccgtt gtcggttcta gttttgcgac
      961 ctcgataccc actggggaag ctattccaat tgccatgagt ccggctaagc aaccgattgt
     1021 ataggtcgcc cataatgcat aaaaatgcct ggttcttatc atgtcatgcc ggcttaactc
     1081 aatcgtggga ttggctgtcg tagtagagga atgggtaatt acattaactg atgaccattc
     1141 atcaggcgga aaacgaaggc atcgtgctaa taatgagacg ataaacaaaa agctgacgcc
     1201 taaatagaaa aaagtattga gggtgccaat ttccgggtga atgatcatcg ctttcatcaa
     1261 aggagcggta atcagtgccg agagaccaaa ccccatcacc gtcaaaccaa tcgccaagcc
     1321 acttttttga gggaaccact tggcgacaac ggcgattggg caaccataca cgatccccac
     1381 accagcaccc ccgatgacgc cgtataaaag tgtcaatgta gtaatatccg gagcaaagct
     1441 agcagccatc cagccaatac ccactaacaa gccgccgagc gttgctgttt ttctgggacc
     1501 ccaatgcgcc atcaagctcc cggcaagcgg catggtgatt gcgaagactg caagaaagat
     1561 catgaatggg taaccgcttt ggctcgatgg aatgtcccac agggtttcta atggttttct
     1621 aaatacactg taagcgtaaa tcgatcccaa gcaaatattg atcgacagtc caattagcac
     1681 aaataaccat cggccttggc ttgctgccat tccgaataat ttcaactctt tttcaatcat
     1741 gctgtttttc ttcgttatat ttttaatcag aaaattttgc atgatcatag cgtttcttaa
     1801 ccggtttggg ggattaaaac gtaaaccaag cattaactta gctggtttta gtttggacgc
     1861 aaaagggaaa gtattttagg attttaggca ctcgcgcctg agagtgggtg ttgggggcaa
     1921 cgatctactg catcgttacc ccccggttag aagaatttaa ttcaagctaa attcagcttg
     1981 ctaactaact gatggtgcca ccgatgtcgg tatcaactaa cagcgtagca gtcccaaacg
     2041 tatattgatc atagttctgc gttccaataa ccacaacacc ggtattggcc cacccttgtc
     2101 cgattgaatt gacaacatca cccactccac catcaactcg cagcgtatcg gtatccgaga
     2161 tcgcgaatac atcggtaaca ttcaacgtga gtgtgttgtt gggcgatggt ccgaatagat
     2221 tgaaaccatt gatattgatt tgctcaatac cggtaatgcg atcatctgcg agtgtggtaa
     2281 gattgagatt ctgtacagca ctgctcatga atgtcaaagt atcagtaccc gatccaccag
     2341 aaattcggtt acccgctacg ctaacatcag ttgcatcgta ccagaaggta tcatcatcat
     2401 tacccccatt caaaatatca gcaccggcac ccccgatgag aacatcatta ccccctgaac
     2461 cggtcaaggt attggtgcca ctattaccaa cgagtgtgtt attaagggaa ttacccgtac
     2521 cattgatatt agctgcaccg gtaagcgtaa gattctcaac gtttgcgata gctgcaaggc
     2581 taaaagtgat agaactttca actctatcag taccggcacc agctgcttca gatatcgtgt
     2641 cacccccatc agcgatatag gtatcgttac cttgcccgcc atcgagcaca tcatttccac
     2701 cgccaccatt caaggtgtcg ttaccaatat cgccactgag cgcattaatc cctgcattgc
     2761 cagtaattac gttatcaagg ctattgccaa gaccattgat attaccaacc ccagttaggg
     2821 tgagattctc aacattggtt ggaagcgtat atgaactcag tgagcttttt acaagatcaa
     2881 tcccttcgtt gagattttca gtgatggtgt cgcccgtata atccatgata taagtgtcat
     2941 tgcctttgcc accttcaagc gtatcattat cgctaatgaa cgtgggacca aagggtccaa
     3001 tgacaacggt gagaccaggc ccaccaatca aagtatcgtt gccatcattt cctttaaggg
     3061 tatcgttgcc cgcaccgcca tcgagtgtat tatcctttga attaccgttg ataacgttac
     3121 ttaaagtatt acctgttccg ttgatcactg ctgttccggt taatgttaaa ttttcaacat
     3181 ttgcaccgct cgtgcctaaa ttaaacgtaa tcgaactttc aacggtatcg gttccttcac
     3241 ctgataattc ggtaatcaca tcggtatttt ccgagatcac gtaggtatcg ttgccaatac
     3301 caccgcttag ggtatcgaca tcaataggtt gaaagaaatt tccagaaccg ccaactagcc
     3361 gatcatcgcc aacatcacca ttgagcacat cattgcccag gccgcctgag agagtatcat
     3421 tgccattgcc gccattgagc gtatcgttac caccggataa tgctaaaaca ccaccatcac
     3481 cattcaacgt gtcattttga tcaccgccgt tgagcacatc attacccaag ccagcattca
     3541 gagtgtcatt accgtttaat ccgtttaaag tgttgttggc gctgttacca gttataacat
     3601 tagcagaaac attaccagta ccacttgttg cagctgcacc ggttaaggtt agattctcga
     3661 tggcagcacc cagtgtatag gttactgaac ttaatactgt atcgattcca ccactatcca
     3721 cgatagtatc gagaggtaaa aaaattccat tggagtcgat aatgtaggta tcgttatctg
     3781 cgccaccttc cagtcggtcg acgccagaac cgccatttaa ggtatcgttt ccgatcccgc
     3841 catacagtgt gtcgttttgg tttccaccca gaatggaatc attaccgcct tccccccaaa
     3901 tcgtgtcact gccatcagcc ccattgacag tatcattgcc cagctgggca aaaatttcat
     3961 taccgccggg tccatcaata tagttgtcat cgagatttgt caaagttacg gtcattttgt
     4021 ggtcctccta ataataatta tttgatcatg cactcaactc tcactggaat aggcgcatgc
     4081 tatgggaagg cttggttaaa cactttcgac tgtggtattg aagcgtttca tttgcaaagt
     4141 atgtgtcaat ggaaagttga attaccaagc aagcacaggt taacttgatc aaattggtaa
     4201 atctgtgaga aaaatcacat ttgtaatcgt ttattgaggt agcttcatcc ccatgtagct
     4261 atctgaactc aactgtggcg ctagaagtgt gataaattca acttgtattt tagcaatgtt
     4321 tttttgaggg caattcgcat gaaattccat atcttcattt taggtctatt cattttggca
     4381 ctcacagcct gttccaccac acggccgatt ttgtatccca atacacatta caaaacagtt
     4441 ggacaacacg ctgctgagca ggatattgtt gcctgccgtc agttagcaga agaagccggt
     4501 gcgcaagaag gtaatgccag ccaggtcggt aatgtcgcga ccagtactgc aatgggtgcc
     4561 gggatgggtg ctgcaagcgg tgcagttggg ggagcgattt ccggtgcagc gggtactggc
     4621 tccttaatcg gtgctgccag tggcgctgcg cttgggttga ttcgtggtct atttggtttg
     4681 gcgagaccga cacaacccaa ccaagcctat gtcaattttg tttatcgctg cttgcaagac
     4741 aagggctatg aagttaccgg ctggcaataa ttgcgagatg aacgaagtgt atgaaaatat
     4801 tgtgtttgaa actgaattcg attgttattc taaacagttc caggaataag tttagcttaa
     4861 agacagtgac tcactcatgt tcagcagaag caagaaacta ggtctgcaat ggctttaatt
     4921 aagaaattac gcgaacaaat gcgaactggt ggtcgcaaat ttgcccgaga tgaaatgaat
     4981 gagtggtaat tttgttgata tcaatatgtt catctactgg ttcgatgaaa ctaataagca
     5041 taaatgcact atttcagagc aattgaattc aaaaagcact gaaaacacat agcgcttgta
     5101 ttagttatca agtaatctaa gaaacactca atattgctac ccgcaagcta tcatccccga
     5161 tgaatgcaga aaatgctgaa gaaagtttta ttctttttgc cagaaggaaa ggatagcgtg
     5221 aattttgtgg tcagtctcac tgtgcatttt ccactacacc actcaccgtg aacagggtat
     5281 tacaaattta gcaaaaatct aactcaacaa atgcaattat catcgatacc tgttatcgta
     5341 tgccctgtgt aattgtttga aaaactcaat aatctagact catgctagcc ggtttaatgt
     5401 taacaatggc gctaatggcg ccagagcttc ccaacccgat tgaaaaagcg gttgattatt
     5461 atcagaaagt agatagctac caaacgatca tcaaatcgta tgtgggaagc aagtccgata
     5521 gtccagacat tgtgcgctac tactacaata aaagtggtgc agtgcgtatg gaagtggtcg
     5581 aaccattcaa tggtgcagtg ctgatttata acccagtgac agaattagtc aaattgtggc
     5641 tatttggtta tggcagcgta ccttctttta gcctaagtcc tgaaaacaaa tggatacaaa
     5701 gttcatcagg acagcgtgtt gaccgatctg atattggtgc attgtatgag gatgtgatat
     5761 tactgcaacg ccaaggcaaa acgactgtcg ttggtaccga aacagtggag aagcaagctg
     5821 cgttgtatgt gatcgtggaa ggcaatcctg gtgtagcgat tgaaggcgta tcgcgtttcg
     5881 atctgtggtt tgacgtacaa accagcttac cgcttaaagt catcagttac aaagcagacg
     5941 ggagtcaaat cgaacgaatc gaaatgtcag aatatcaagt taaccctgcc ttcccaaaag
     6001 gtttttttga tcattaattt tagtgacggc tgcattttct tttactactg aatggcgctt
     6061 cgatgccccg cacgaaatgg tatgtgatgc aatcattcaa tgcctggatt ggcctcagtg
     6121 gtggttaggg gctgaacaag tggaaaaact ggtaacaggt gatagcgatg gcgttggtag
     6181 tgtgcatcgt ttcacttgga aaggtaaaat tccttatcat tttaccttta cgatacgagt
     6241 aacgcgctat gtacccggtg ttttgctaga aggggtagct gaaggtgaag tggtcggtac
     6301 cgggcgctgg gatttcatac gcgcgaacga tataacgatc gtccgctatg cctggacagt
     6361 gcgcacaaac cgtctatgga tgaatctgct tgcaccgatc gcgagaccga ttttcaaatg
     6421 gagccacgat caagtaatgc ggcaaggtga actcggatta gcgcactggt tacgcatgca
     6481 atctaagtaa aatgccggct actttgtgca agccagcaga aaagacatca aacaaatcca
     6541 acaacctaga cttactattt cttagatttc gttacagagg gtacttctgt tggctgctgt
     6601 acctgaaaaa agacttcatc tttacgtatc attcctagct cgtttctcgc aagctcttca
     6661 atagcgtcaa aaccctgctt gagatcaatg atctctgctt ccaggatggt attctggttc
     6721 tgtgatttct gatttaattg gtgttgatct tggagttgtt tctccaactc aaagacttcc
     6781 aaccacccac ctttaccata ccacagggaa tattgaacta atgcaattaa agtaaccagg
     6841 atgagcgcaa cagtcttcat taggttaatt gatagaatgc actccttcct gcataaattg
     6901 cgttatcacc caagtcttct tcgatacgaa gcaactggtt atattttgct aatcggtcgg
     6961 aacgtgacaa tgaacccgtt ttgatttgaa gtgcattcgt tgcgactgcg atatctgcaa
     7021 tggtcgtatc ctcggtttca cccgagcgat gcgaaatcac agccgtgtag cctgcacatt
     7081 tggcgatttc tactgcagtc aaagtttccg tcaacgtacc aatctgattc actttaatca
     7141 gaatcgaatt tgcaataccc cgttgaatac cctcttttag tatccggcta ttagtcacaa
     7201 aaacatcatc acccaccaat tggacagttt ttccaagcct ttcggtcaat aatttccagc
     7261 catcccaatc atgctcactc ataccatctt caatgctgat aattggatat ttatcgaccc
     7321 aggtagccaa ataatcgaca aattgggcag aagtcaggct taaaccatcg gatttaagat
     7381 ggtattttcc atcttggtag aattcagaac ttgcacaatc aatcccaatc gcaacttgtg
     7441 taccaggttg ataaccagct tgatcgatgg cttgcacaat caactgtaat ccttcttcat
     7501 taccagacag attcggtgca aacccccctt catcacccac cgttgttggc atattttttt
     7561 tactgatcag ttttttcaat gtatgaaaaa tctcagcccc acaccgcagt gcttcacgaa
     7621 agctgtcaaa accatgcgga atcaccatga actcctgcat attcaaatta ttattggcgt
     7681 gagcaccacc attgatgaga ttcatcatcg gaaccggcat cgatatcgct cttactcccc
     7741 ctaaatatcg atataaaggc aaactggatt cttctgcagc ggctttcgcc actgccagcg
     7801 aaact
//
LOCUS       DAOQLJ010000108         7803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3759_length_7803_cov_7.558596, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000108 DAOQLJ010000000
VERSION     DAOQLJ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7803
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3759_length_7803_cov_7.558596"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..936)
                     /locus_tag="PK522_08190"
     CDS             complement(82..936)
                     /locus_tag="PK522_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNC41750.1"
                     /translation="MSNYVQRFLLENLAIRGSVVHLDSIWLQMTAGRNYPQPAAQLLG
                     EMSAITLLLGDNLKQSGRLTIQLTGNGPISMLVLDCTENLHLRGMAKCAPLVEAQPVP
                     DLLGDGRLLLTLDMPSMRESYQSIVPLEGNNIAEIFEHYFLQSEQLPSRLFLIAEHQS
                     IFGLLLQKLPNADLQDPDGWVRAEALAATVQDHTLLDLSAEQILMRLFYEETIRIFDP
                     QPVHYGCKENPTKIYTMLRSLGREEVDSILKEFGEVIVNDDICNREYRFDALAVDAIF
                     REAGPTVH"
     gene            complement(1006..1227)
                     /locus_tag="PK522_08195"
     CDS             complement(1006..1227)
                     /locus_tag="PK522_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41751.1"
                     /translation="MTGLSNKIEVLTHEQLTCVSGGMIKLDRLPHIPPDQPHGGGLPV
                     DYWATAGTMRFGAGPWDYGYGSPTGPWPV"
     gene            complement(1445..2911)
                     /locus_tag="PK522_08200"
     CDS             complement(1445..2911)
                     /locus_tag="PK522_08200"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNC41752.1"
                     /translation="MKPTLQLKLTPNLTLTPQLQQSIRLLQLSTHELNQEIERMAQEN
                     PLLELENTIHEQPYRDDAPSESQSSPTLSNEEGVQSEDSYLNNNELSPSSASEIELEW
                     YGDQDSPYTKRSDDDDEWDLFQQTAESISLREHLATQISLSQIDDRERRIVGLLIDSL
                     DDAGYLSQDLMELSELIPSELGIQLKDLETALKHLQRLDPPGIGARNLRECLTLQLQS
                     LPETTPYRDKALQLVMTDLECLAAKDFRQIKKMLQCDDHCLRAIQQLIIHLNPRPGEA
                     YKNDFARYIVPDVIAAKSEGAWIAKLNPSSLPRLSINHLYANILKHNKNESTQSLIGQ
                     LNEAKWLIKNIQQRSDTILRVSNEIVTRQQDFFENGAVSMRPLIMREIAESLELHEST
                     VSRVTTQKFIHTPHGIFELKYFFSSHVATDTGDTCSAVAIRSLIKQLIQVENQKKPLS
                     DLQISKILGERGIVVARRTVAKYREFLHIPPANLRKIV"
     gene            complement(2946..3668)
                     /gene="lptB"
                     /locus_tag="PK522_08205"
     CDS             complement(2946..3668)
                     /gene="lptB"
                     /locus_tag="PK522_08205"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNC41753.1"
                     /translation="MSILSAKNLKKSYKSRTVVKDVSFSVASNEVVGLLGPNGAGKTT
                     CFYMIVGLVPLCGGEIYLDDRNLSKLPIHERARLGLSYLPQEASIFRRLSVEKNILAV
                     LELQNLSADEIQQRLDNLLHDLHISHLRENMGISLSGGERRRVEIARALASQPNFVLL
                     DEPFAGVDPIAIIDIQKIIRFLKDQGIGVLITDHNVRETLGICDRAYIISDGTVLASG
                     KPDEIINNEQVREVYLGEHFRL"
     gene            complement(3702..4232)
                     /gene="lptA"
                     /locus_tag="PK522_08210"
     CDS             complement(3702..4232)
                     /gene="lptA"
                     /locus_tag="PK522_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HNC41754.1"
                     /translation="MVVGVLPSSLALAERADRNKPIQLESDSATVEDYKRKDTFRIST
                     FVGNVILTQGTIVIKADKIIMKEDLKGYRYATAHGDLVSFRQKRDGANEFIEAWSQRV
                     EYDDKTDKIELFGKARLKRGPDEVKGDYISYDVARDFFQVKGNNTKNEVENPDRRVRV
                     VIQPKATPKIEDDSEN"
     gene            complement(4324..4890)
                     /gene="lptC"
                     /locus_tag="PK522_08215"
     CDS             complement(4324..4890)
                     /gene="lptC"
                     /locus_tag="PK522_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNC41755.1"
                     /translation="MIRRLLPGPVIILAILVLFTFWLDQSIQQPKKRYDTDQTKNPDY
                     VIENLSGIQMVYAQAAERHFTAKILKHYPVTNVSALEHVHFTHNQTNEPTLKINADSA
                     ELSKGNEDIYLFGNVNVVRGDEANKDIVTMVTDSLHLVPDQEIVKTDRPVTITKMNTI
                     VNSVGMQLNNKTGIIELHSKVKARDVKL"
     gene            complement(5186..6847)
                     /locus_tag="PK522_08220"
     CDS             complement(5186..6847)
                     /locus_tag="PK522_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNC41756.1"
                     /translation="MEIQANPVNISKDLAITTEETLTDPIPEPILKGHLRLRLKLIAQ
                     GLTAPNWAIPAPGDTDHLYVGDQNGKLWKINLLDGNKESFLDLSQLLVPLGIFGENSF
                     DERGFLGFAFHPQYTQNSLLYTYTSEPAGEKSDFSTISSGSNPNHRSVIREWRINSAN
                     LAKDMPNRVLLSIDQPQFNHNAGALNFGPDGMLYIALGDGGGADDRDGQPFIGEPIIG
                     HGTHGNSQDLTNPLGSLLRIDPSGTNSINGQYGIPNDNPFSNSNNALGEIYAYGFRNP
                     FRFSFDSVSGALLLADVGQNTIEEVNIVKSGGNYGWGLKEGRFRFEPNGNGPGFITNA
                     PIIGDFIDPILEYDHDEGTAIIGGFVYQGKMIPELKGHYVFGDVAKTSNGDGRLFYSD
                     GSKIQELDLTQQSQPGFWILGFGQDNDGELYVLGNSTGLPFGSTGVVYKIVPNSHFDG
                     TEVAIPAVKVITSIDKAETYHARLRLIPDSQPLRFELIHAEKLPDRIIDTDNAIFNSA
                     MGTLNLPFINIVSQGSEVTTYQVQLRQVSNQPTLTFELTQSVLIR"
     gene            7061..7402
                     /locus_tag="PK522_08225"
     CDS             7061..7402
                     /locus_tag="PK522_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNC41757.1"
                     /translation="MKATVTELLQRMPGKVSEEWPMGEPFAVALAHGSMSVELYAPKD
                     VDIQTPHDQDELYFIHSGKGEIVIAGSRYAFEPGTVFFVPAHIEHRFENFSADFATWV
                     VFWGPKGGEKA"
     gene            7453..>7803
                     /locus_tag="PK522_08230"
     CDS             7453..>7803
                     /locus_tag="PK522_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41758.1"
                     /translation="MFQRKIMLVTILFTFLLVDVTAAPVPVGPALMGSGDGLKMRWVN
                     TDFSPHSITDAAQALNLRPGDVGYVGEVSGVTKVIDFEDGNYGDLFRDTDQAVPLGAD
                     DNFAVLYKGFLNVTV"
BASE COUNT         2086 a         1555 c         1754 g         2408 t
ORIGIN      
        1 ttccccattg attttccatt gattttcctt gttggtattt ttgttgcgcg gaactttgta
       61 tctcatgaaa ttcagcattc ctcaatgtac tgttggtcct gcttcgcgaa agattgcatc
      121 gactgcaagc gcatcaaacc gatattcacg gttgcagatg tcgtcattga caatgacttc
      181 tccgaattct ttcagaatcg aatcgacttc ttcgcgaccc agcgagcgta gcatagtgta
      241 aatcttggta gggttttcct tgcagccata gtgaactggt tgtgggtcga aaatacggat
      301 ggtttcttca taaaataaac gcatcaagat ttgttctgcg ctgagatcaa gcagtgtgtg
      361 atcttgaaca gtggcagcaa gtgcttcagc acgcacccaa ccgtccggat cttgcagatc
      421 agcattggga agcttttgta gcagcagacc aaaaatagac tgatgttcgg cgatcagaaa
      481 caggcgcgat ggtagttgtt cggattgaag aaagtaatgc tcgaagattt cagcaatgtt
      541 gttaccttcc agcggtacga tgctttggta gctttcgcgc attgagggca tatcgagtgt
      601 aagtaataat cgaccatcac caagcagatc gggaactggt tgtgcttcga caaggggagc
      661 acactttgcc atgccccgga gatgtagatt ctcggtacaa tcgagtacca gcatcgatat
      721 tggtccgttg ccagtgagct ggatagtgag tcgtccagat tgtttgaggt tatcgccaag
      781 cagtagggta attgcactca tttcgccaag tagttgtgcc gcaggttgtg ggtaatttcg
      841 tcctgcggtc atttgcaacc aaatcgaatc gagatgtact accgaaccgc gaatagccaa
      901 gttttccagc aggaagcgct gcacgtagtt gctcatgatg taatgcttga tcctattaat
      961 tttctgatac cagagtggtc gattatagcc actctggtcg attaattaaa ctggccaggg
     1021 tccagtgggg gaaccataac cgtaatccca agggccagca ccaaagcgca ttgtgccagc
     1081 ggttgcccag tagtctaccg gaagaccacc tccatggggt tgatcaggtg gtatatgcgg
     1141 gagtctgtcg agtttaatca tcccgccact gacgcaggtt aattgctcgt gggttaatac
     1201 ttcgatttta ttggaaaggc cagtcatgat tgcttctcct attgaattga gttaataaat
     1261 tttcaggcca tatttggatg gcagtattaa aatagcgatg cttagccaat gaagctgtga
     1321 taaaactcac gttcaagcgg tattgacttc aatttatagg agggacgaga agattatccg
     1381 tttatcttga gtaaccgcaa tgttgcgcgg tgagtatcgg ataattttga tgactgagaa
     1441 gtatctagac gattttgcgg agatttgcgg gtggaatgtg gagaaactct cgatatttgg
     1501 caacggtgcg gcgagcaacg acgatacccc gttcacctag aatttttgat atttgaagat
     1561 cgctgagtgg tttcttttga ttttcaactt gtattaattg tttgattaag cttcgaattg
     1621 caacggctga gcaggtgtca ccggtatccg tcgcgacatg gctactgaaa aagtatttca
     1681 gttcaaaaat accatgcggg gtatggatga atttttgagt agtaacgcgt gaaactgttg
     1741 actcatgaag ctcgagtgat tctgcaattt cgcgcatgat cagcggtcgc atagaaactg
     1801 caccattttc gaaaaaatct tgttgccgcg taacaatttc gttggatact ctcagaatcg
     1861 tatctgagcg ctgctgaata tttttgatga gccatttggc ttcattgagt tggccgatca
     1921 aagattgagt ggattcattc ttattgtgct tgagaatatt tgcatagaga tgattaatac
     1981 taagccgtgg gagtgaactg ggattgagct ttgcaatcca ggctccctcg gatttagcag
     2041 caatcacgtc aggaacgatg tagcgagcaa aatcattttt atatgcttcg cctggccgtg
     2101 gattgagatg aatgattagc tgctgtatgg cacggaggca atgatcatcg cactgcagca
     2161 ttttcttgat ctgtctaaaa tccttcgctg ctagacattc gagatcagtc ataaccagtt
     2221 gtaaagcttt atcacggtaa ggtgttgttt ctggaagtga ctgtaactgg agcgtgagac
     2281 attctcttaa attacgtgca ccgatcccag gtggatcgag tcgttgcaga tgtttaagcg
     2341 cagtttcaag atcttttagc tgaataccca attcagaagg aatgagttct gaaagctcca
     2401 ttaaatcctg acttaaatag cctgcatcat caagactatc aatcagtagt cctacaattc
     2461 tccgttctcg atcgtctatt tggctgagac taatttgtgt tgcgagatgc tctcttagac
     2521 taatcgactc agcagtctgt tgaaataaat cccactcgtc atcgtcatcg cttcgtttag
     2581 tataggggct atcttgatcg ccataccatt caagctcaat ttcagaagcg ctacttggcg
     2641 ataattcgtt attattgaga tacgaatctt cactttgaac gccttcttca tttgaaagtg
     2701 ttggtgaaga ttggctttcg ctgggtgcat catcccgata gggctgttca tgaatcgtat
     2761 tttctaactc taacaaggga ttttcttgag ccatgcgctc gatctcttga ttcaattcgt
     2821 gagtggaaag ttgaagtaaa cgaatcgatt gctgtaattg aggcgttagc gttagattgg
     2881 gggtgagttt tagctgtagt gttggtttca tgatctaaat ttcacttttc ttagcttatt
     2941 catgcctaaa gccggaaatg ctcccctaga taaacttctc taacttgctc attgttgatg
     3001 atttcgtctg gttttccact cgctagaacc gttccatcac taattatata agcacgatca
     3061 caaataccaa gtgtttctcg tacgttatga tctgtaatca gaacgccaat gccctgatct
     3121 tttagaaagc ggataatttt ctggatgtcg ataatggcga taggatctac ccccgcgaat
     3181 ggctcgtcga ggagtacaaa attgggttgt gaagctaagg ctctagcaat ttctactctg
     3241 cgacgttccc cacctgacaa actgatgccc atattttctc gtaaatgact aatatgcagg
     3301 tcatgtagta gattatctaa ccgctgctgt atttcatccg cacttaaatt ctgtagttcc
     3361 agaacggcta gaatattttt ctcaaccgac aagcgtctaa aaattgaagc ttcttggggt
     3421 aagtaactca atcctaagcg agctcgctcg tgaatgggta atttacttag attgcggtca
     3481 tccaggtaga tttcaccacc acataatgga actaaaccga cgatcatata gaaacaagtt
     3541 gttttgccag ccccattggg ccctaataaa ccaacaactt cgttgctagc gaccgagaag
     3601 gaaacatctt tgacgacggt tctcgattta tagctttttt ttaaattttt agcgcttaag
     3661 atgctcatgg ataaacgatt gtctgttata aactaaaaaa tctagttttc tgagtcatct
     3721 tctatttttg gagttgcttt gggttgaatg acaacgcgta cgcgacgatc tgggttctca
     3781 acctcgttct tcgtattatt tcctttaacc tggaaaaaat ctcgagcaac atcataagaa
     3841 atataatccc ctttaacttc atctggacct cgctttaatc gcgctttacc aaataattcg
     3901 attttgtcgg ttttatcatc atattcgaca cgttggctcc aggcttcgat aaactcattt
     3961 gcgccgtctc ttttttgtcg gaaactgacg agatcgccat gggctgtcgc atagcgatag
     4021 ccttttagat cttctttcat aattatttta tctgctttaa tcacaatggt tccttgggtg
     4081 aggatgacat tgccaacgaa cgtgctgata cgaaaggtgt ctttgcgttt atagtcttcg
     4141 accgttgcgc tatcagattc taattgaatc ggtttgttgc gatctgcgcg ttcagcaagc
     4201 gccaaactag agggtaatac accaacgacc atagcgagca agcagctaat caaatgtacc
     4261 cgcttaaaag tgggataaat caaagtatcg cgattacgca agatcgattt catttttttt
     4321 gcttcatagt ttgacatccc gcgcctttac ttttgagtgg agttcaatga tgcccgtttt
     4381 attattaagc tgcattccta ctgaattcac aatggtattc atttttgtta tggttaccgg
     4441 ccgatctgtt tttacgattt cttgatcggg aactaagtgc agcgagtcag ttaccatggt
     4501 aacgatatct ttgttggctt catcacctcg aacaacatta acattaccaa ataagtaaat
     4561 atcttcattc cctttggaaa gctcggctga atctgcatta atttttaatg tcggttcatt
     4621 ggtctggtta tgggtaaagt gaacgtgctc aagcgctgaa acattcgtca ctggataatg
     4681 cttcaaaatc ttggcagtga aatgccgctc tgcagcctgc gcatagacca tctgtattcc
     4741 tgacaagttt tcgataacat aatcaggatt tttggtttgg tcggtatcgt aacgtttttt
     4801 aggttgctga atggattgat ctagccagaa tgtgaataag acaagaatgg ctaatataat
     4861 tacggggcca ggtaataagc ggcgaatcat tttattagta attcgtgaga atgatgactt
     4921 agatgaatag ggtttaataa attaatctga ctattgattt aatggttata aattttaaca
     4981 aaacataaaa aaaataactg tatgagcagt aaagtaatta atgtttattt aatgcatatc
     5041 agtgaagcaa atcaactgaa ttaagtagtg gagtttaaat atgggacaac taagcagtaa
     5101 aaagcagtct ttccgctcgc ttttgtttac tggatcctta tgaatggtgg atactattat
     5161 aacactttgt aacctatcct gattattagc gtatcagtac tgattgagtt aattcaaaag
     5221 tcagcgttgg ttgattcgat acttgtcgaa gctgcacctg gtaggttgtt acttcactcc
     5281 cttgcgagac gatattgata aacggtaagt ttagtgttcc cattgctgag ttaaatattg
     5341 cattgtcagt atcaataata cgatctggta gcttttctgc atgaatcaat tcaaagcgta
     5401 gcggttgact atctggaatg agccgaaggc gagcatgata agtttcagct ttatcgattg
     5461 aagtgataac cttaactgct ggtattgcaa cctcagttcc atcaaaatga ctattgggaa
     5521 caattttata aacgacccct gttgatccaa aggggagtcc tgtgctattt ccaagtacat
     5581 aaagttcgcc atcattatcc tgaccaaaac cgagtatcca gaacccaggt tgcgattgct
     5641 gggtcaaatc gagttcttga attttactgc catctgagta gaagagtctg ccatctccat
     5701 tgcttgtttt ggcgacatcg ccaaaaacat aatgtccttt caactcgggt atcattttac
     5761 cctggtagac aaaaccaccg ataatcgcag tcccctcgtc atgatcgtat tccaagattg
     5821 gatcgataaa atcaccaatt atgggggcat tggttataaa acctgggcca tttccatttg
     5881 gttcaaagcg aaagcgacct tcttttagtc cccagccata atttcctcct gatttaacga
     5941 tattcacttc ttctatggtg ttttgaccga catcggctaa tagtagagca cctgatacgg
     6001 aatcaaatga aaagcggaac ggattgcgaa atccgtaagc atagatttca cccaacgcat
     6061 tattactgtt agaaaaaggg ttatcatttg gaatgccata ttgcccattg attgaattgg
     6121 ttccactggg atcgattctg agtaggctac ctaacggatt ggttaaatcc tggctattgc
     6181 catgagtgcc atgcccaatg atgggttccc cgataaaagg ttgtccatcg cggtcatcgg
     6241 cgcccccgcc atcgccaagg gcgatataaa gcatcccatc tggaccaaaa tttaatgcac
     6301 ctgcattatg attgaattgc ggttgatcga tactcagtaa aacccgattg ggcatatcct
     6361 ttgctaaatt agcggagttg attctccatt cccggatgac ggagcggtga ttggggtttg
     6421 agccagaaga gatcgttgag aaatcacttt tctcacccgc tggctccgaa gtataagtgt
     6481 agagtaagct attttgcgta tattgagggt ggaaggcaaa accgagaaaa cctctctcgt
     6541 caaatgaatt ttcaccaaat atgccgagtg gcacgagtag ttgagaaaga tcaaggaaac
     6601 tttctttgtt gccatcaagc aagttgattt tccatagctt tccattttga tcacctacat
     6661 agaggtgatc ggtatcccct ggtgctggga tagcccaatt gggtgccgtc aacccttgtg
     6721 ctatgagttt tagtctcaga cgtagatggc cttttaaaat cggctcagga attggatcag
     6781 ttagcgtttc ttcagtagtg atggctaaat ctttagaaat gtttactggg ttagcctgta
     6841 tttccatgct atacgtgcca atagctaaaa atagcaacga taatgtgctg cgtatcataa
     6901 tacttacccc ccttgagcgg ataaacgctt actcatcgat catactccag tatagatgaa
     6961 tccatactta tttattttat ttcatagagt tattgcttga aatttttttg actatctgga
     7021 taatgttgca tatgacatat tttttatgga aacatgctgt atgaaagcta ctgtaactga
     7081 acttttgcaa cgtatgcctg gaaaggtttc tgaggaatgg ccaatggggg agccttttgc
     7141 cgttgcactt gcacatggtt cgatgtcggt agagttatat gcgccaaagg atgtcgatat
     7201 tcaaactcca catgatcagg atgaactgta ttttattcat tccggaaaag gtgagatcgt
     7261 gattgcgggg agtcgctacg catttgaacc tggtacggtt ttctttgttc cagcgcatat
     7321 tgagcatcgt tttgaaaatt tttctgctga ctttgcaacc tgggttgttt tctgggggcc
     7381 caaaggggga gaaaaagcgt aatgggtgtt gcaaagaaaa ttttacctcg atcgaacgtc
     7441 aaaggaggcg tcatgttcca aagaaaaata atgctagtca caatactgtt cacttttttg
     7501 ttggtagatg tgacagcagc gccggttccc gttggtccag cgctaatggg aagtggggat
     7561 gggttaaaga tgcgttgggt aaatacggat tttagcccgc actcaataac agatgctgct
     7621 caagccctta atctcaggcc aggtgatgtg ggctatgtgg gagaagtcag cggcgtcaca
     7681 aaagtcatcg actttgaaga cggtaattat ggagatttat ttcgggatac tgaccaggcg
     7741 gtcccgctcg gcgctgatga taattttgct gttctttata aaggttttct gaatgttact
     7801 gtg
//
LOCUS       DAOQLJ010000109         7799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3760_length_7799_cov_6.277505, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000109 DAOQLJ010000000
VERSION     DAOQLJ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3760_length_7799_cov_6.277505"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2286
                     /gene="pheT"
                     /locus_tag="PK522_08235"
     CDS             <1..2286
                     /gene="pheT"
                     /locus_tag="PK522_08235"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNC41759.1"
                     /translation="VDEVASAFDNVVVAEVISVQKHPDADRLTICNVDVGLANNTLLQ
                     IVCGAANVSESIKVPCALVGACLPGGIVIKQSKLRGVESFGMLCSAQELGLGEKKEGL
                     LVLPGDAPVGMNFRDYYQLEDRIFTLKLTPNRADCLSLNGIAREVAAITDTPLHVPEY
                     ELARDSCVDRLTVNVEVPDACPLYCGRIIRNVTPATPTPLWMVHRLERSGIRAINIVV
                     DILNYVMLEMGQPMHGFDLAKISDQLLGKVVVRFAHSDETLKLINGENLELKSDMLVI
                     ADTNRAVALAGIMGGLETSIQNETTDVFLESAYFSPHVISGKSFKLGFNTDSAYRFER
                     GVDFASTRKALERATQLILELMDDAVAGPITQVESSLPQRSPVMVRLSKIQQVLGINL
                     NSNQIASILERLRFSFSEDKGIFHISPPSYRFDLAIEVDFIEEVARIYGYDRIPISLP
                     KTTVSMLPDSELGRLSPSQLRNMLVLRDYQEVINYAFVDPQWELDFSKDCIPIALKNP
                     IASQMSVMRTTLIGGLIANLQFNISRKQSRVRLFEIGCCFLRGAGDNYQQIEKIAGIC
                     YGDTLEEQWGVTSRNVDYFDIKMDIESVYLPQKVTFNKCEHHALHPGKSAQIIIDNHV
                     VGWLGELHPRLQTQYDLPRPVILFELSLDSLDRKHLSKAKVLSKFPPVYRDIAIVISE
                     HVTNDQLLKTMKDEQLHIVKDIYLFDYYSGKSLEQGKKSYAYRILLQDNEKTLTDQEV
                     EVAILKLISVLQAKYDAKLRV"
     gene            2560..2874
                     /locus_tag="PK522_08240"
     CDS             2560..2874
                     /locus_tag="PK522_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HNC41760.1"
                     /translation="MALTKAELTDLLFESVGLNKREAKEVVESFYEELSLALQKGEGV
                     KLSGFGNFQLRTKPCRPGRNPKTGEEIPISARRVVTFHASQKLKSMVEFNYHRKNGAR
                     QP"
     gene            2855..3235
                     /locus_tag="PK522_08245"
     CDS             2855..3235
                     /locus_tag="PK522_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNC41761.1"
                     /translation="MERDSRSYPPIPAKRYFTIGEVSELCGVKPHVLRYWEQEFIQLR
                     SIRRRGNRRYYQHHEVLLIRRIHELLYGQGFSISGARTRLSNDGGLESGLIEDPSQTI
                     TACSTEELLYIRKEIKAIISLLRS"
     gene            3293..3369
                     /locus_tag="PK522_08250"
     tRNA            3293..3369
                     /locus_tag="PK522_08250"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3327..3329,aa:Pro,seq:ggg)
     gene            3412..4161
                     /gene="surE"
                     /locus_tag="PK522_08255"
     CDS             3412..4161
                     /gene="surE"
                     /locus_tag="PK522_08255"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNC41762.1"
                     /translation="MRILLSNDDGYFAPGLAHLANALSTIAEIVVVAPERDRSGSSNS
                     LTLDRPLKLHKSPNGFYFVNGTPTDCVHLAVTGMLDYLPDMIVSGINDGANMGDDTIY
                     SGTVAAATEGFLFGIPSIAVSLVNRSECNFATAAQVALEMVKRFMEKRVHVPVLLNIN
                     VPDVPLSRLAGMEVTRLGRRHKAEPVIKSTNPRGETIYWVGAAGIAQDAGEGTDFFAV
                     QNNKISITPLQIDLTRYDQIGYLKDWLDLSD"
     gene            4230..4865
                     /locus_tag="PK522_08260"
     CDS             4230..4865
                     /locus_tag="PK522_08260"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNC41763.1"
                     /translation="MTSQRTRARMVERLREQGITDEMVLAVMNTIPRHLFVEEALASR
                     AYEDVSLPINFGQTISSPWIVARMTELLRGNHNLNKVLEIGTGCGYQTAVLAKLAKEV
                     YSIERIGPLLTRTRIRLRELRITNISLKHADGLLGLPDVGPFDAIIMTAVTTQIPELL
                     LEQLTVGGRMVYPKGISKQFLCVIEHTSQGFIETILDEVKFVPILSGVVNR"
     gene            4922..5983
                     /locus_tag="PK522_08265"
     CDS             4922..5983
                     /locus_tag="PK522_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNC41764.1"
                     /translation="MFGASTKMCFGLNVNRYILVTDIYQVLLLLLMCWVVTGCVSPHT
                     APIVESQQNPTRAPSPEVVKPRIQNKQLYVVQRGDTLYGIALKHGVDYKELVQWNGIA
                     DPNTLKVGQEIDLLVPSRQSKVAEIAPEDQSAQPTLFALPQSAAPVEVNSIKPLQTEP
                     VSPSPGAMVIAEPKSIKLPYAEPSAAKPDLEVSQSTKNQPAAILPPPNKIEKPSDVEK
                     PTKAQPEIALPKNNNNSIKWSWPTNGKVLSKYTERTKGVSISGNKGQSVLASADGTVV
                     YSGNSLRGYGNLIIVKHNDIYLSAYGHNSKILVREGETVSKGQKIAEMGNTDSETVKL
                     HFEIRERGKPVDPLKFLSN"
     gene            complement(6046..6564)
                     /locus_tag="PK522_08270"
     CDS             complement(6046..6564)
                     /locus_tag="PK522_08270"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HNC41765.1"
                     /translation="MTYIFSFEPIADHHASVLILGSMPGNASLMAGQYYAHKQNVFWK
                     IMAELLQFELNSSYETRVQKLKSAHIALWDVLRSCKRVGSLDSNILPNSQTVNNLQHF
                     LQQHHLISKVCFNGSKAENCFNRYVAKTISGSPKQFIRLPSTSPANASIPYEKKLKIW
                     QEALFPSLRSNL"
     gene            complement(6628..>7799)
                     /locus_tag="PK522_08275"
     CDS             complement(6628..>7799)
                     /locus_tag="PK522_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNC41766.1"
                     /translation="YTDLVSGKMTVSQIRDIELDDLLGREPVILDAEGLHTLLSNKTI
                     LITGAGGSIGSELCRQIITFKPKQLVLFELNEFALYSIQEEFCSKFPDIAMAFVIGDV
                     KDESRLAQLFAQYQPTVIFHAAAYKHVPLMEKENTWQALQNNVLGTYVLAKLAIKHHI
                     TKFVLISTDKAVNPTNVMGASKRLAEMVCQALQQSILSQESMQANTIFSPSTRFVIVR
                     FGNVLGSTGSVIPKFREQIARGGPITVTHPDISRYFMSIQEAAQLVLQAGSMGGIKGG
                     GEIFVMDMGNPIRIVDLAKDMIRLSGFSEEEIKITYTGLRSGEKLHEELITENESTLP
                     TQHEKLRITLSHPVDERWLTSFITWLIEQPALSDTEVRMALAKWVPEYSDKNNEH"
BASE COUNT         2329 a         1382 c         1750 g         2338 t
ORIGIN      
        1 gtggatgagg ttgcgtctgc atttgataat gtagttgtag ctgaagttat ttcggttcaa
       61 aaacatcccg atgcagaccg actaacaatt tgtaatgttg atgtagggct agccaacaat
      121 acattacttc aaatcgtttg tggtgcagca aatgtatctg aaagtatcaa ggttccctgt
      181 gctttggtgg gcgcatgtct gcctggtgga atcgtcatca agcaaagcaa attaagggga
      241 gtggagtcat ttgggatgct gtgttcggcg caagaactag gactgggtga aaaaaaggaa
      301 ggattactcg ttttgccagg ggatgcgcct gtaggcatga attttcgcga ctattatcag
      361 cttgaggatc gtatctttac gcttaaatta acgcctaatc gtgctgattg cctaagtcta
      421 aatggcatcg cacgcgaggt ggctgcgatc accgatacgc cattgcatgt tcctgagtat
      481 gaattggcta gggatagttg tgtagatcgt ttgacggtca atgtggaagt gcctgatgcc
      541 tgcccgctat attgtggtcg tattattcga aatgttacgc cagcgactcc tactccttta
      601 tggatggttc atcggttaga acgaagtggg attcgtgcta tcaatatcgt ggtggacatt
      661 cttaattatg tgatgttaga aatgggtcag ccgatgcatg gttttgattt agctaaaatt
      721 tcagaccaat tattgggaaa agtagttgtt cgttttgcgc attccgatga aacactcaaa
      781 cttattaatg gagagaatct tgagctgaaa tcggatatgc tggtaattgc agataccaac
      841 cgggcagttg cattagctgg aataatgggt ggactggaaa cgagtattca gaatgaaact
      901 actgatgtat ttctagaaag tgcctatttt tctcctcacg taatcagtgg aaaatcattc
      961 aaactaggat ttaataccga ttcagcatac cgctttgaac gaggagttga ttttgcatca
     1021 acccgaaaag ccttggaaag ggcaactcaa ctaattttag aattaatgga tgatgcagta
     1081 gcggggccaa taacgcaagt tgaaagctca ttaccacaac gaagtccggt tatggtgcga
     1141 ctttcaaaaa tacagcaagt cttaggaatc aatttaaata gcaatcagat tgccagtatt
     1201 ttagaacgat tgagattcag ctttagtgaa gataagggca tttttcatat atcgcccccg
     1261 tcttatcgtt ttgatttagc gattgaagtg gattttatcg aggaagtcgc tcgtatatac
     1321 ggatatgatc gtattccaat cagcttacca aagacgacag tgtcgatgct tcctgattca
     1381 gaattgggaa ggctctcacc ctctcagctt aggaatatgc tggttttgcg tgattatcag
     1441 gaagttatta actatgcatt tgtagatcct caatgggagc tcgatttttc taaagattgc
     1501 atccctattg ctttaaagaa ccctattgcc agtcaaatga gtgtcatgcg gactacattg
     1561 ataggagggc taattgccaa cttgcagttc aatatcagtc gcaagcaatc tagagtgaga
     1621 ttatttgaaa ttggatgctg ttttttacgt ggtgcaggag ataattatca acaaatcgaa
     1681 aaaattgctg gaatttgcta tggagatacc ctcgaagaac agtggggagt gacatcgcga
     1741 aatgtagatt attttgatat taaaatggat atcgaatcag tgtatttgcc acaaaaggta
     1801 acttttaata agtgtgagca tcatgcacta catcctggaa aatcagcgca gattatcatt
     1861 gataaccacg tagtggggtg gcttggagag ttacatccac gcttacaaac tcaatatgat
     1921 ttacctcggc ctgtaatttt attcgagttg agtttggatt ctctggatag aaagcattta
     1981 tctaaggcta aggttctttc taaatttcca cctgtttatc gtgatattgc gattgttatc
     2041 tctgaacatg tgacgaatga tcaattactt aaaacaatga aagatgagca acttcatatt
     2101 gtcaaagata tttatctttt tgattattac agcggaaaat cgctagagca aggtaagaaa
     2161 agttatgcat atcggatttt attgcaagat aacgagaaaa ccttaactga tcaagaagtg
     2221 gaagtggcaa tcttaaaact aatcagtgtg ctgcaagcta aatatgatgc aaagcttaga
     2281 gtgtaatttc ttcgcttcga tagtaaaata ttctctatcg tattttgatt gattaaaagt
     2341 gctgttgagt taagaatgag tttaggctat caatcgcaag taacataaaa tccttgttca
     2401 agttttcgaa ctgaatctct gcatataaaa aaagagtcaa attctaatag caataaagca
     2461 gtctatcgaa gcgttgtttt gtttctaaaa acctaaaact ttgggtaaat gctggatata
     2521 ggttgtgtat tcgtgtgatt taaatttaat gagggagata tggcattaac aaaagcggaa
     2581 ttaactgatt tgttgtttga aagtgtagga cttaataaac gcgaggcaaa agaagtcgtt
     2641 gaatcttttt atgaggaatt aagtcttgcc cttcagaaag gagagggagt taagttatca
     2701 ggatttggta attttcagtt gcgtactaaa ccctgtcggc ccggtagaaa tcctaaaaca
     2761 ggtgaagaga ttcctatttc agcgaggcga gtcgttactt tccatgcaag tcaaaaatta
     2821 aaatcgatgg tagagtttaa ctatcataga aaaaatggag cgagacagcc gtagttatcc
     2881 tcctatccca gcaaagcgct atttcacaat tggagaagtg agtgaattgt gtggtgttaa
     2941 gccccatgtt ctgcgctatt gggagcaaga atttattcaa ctcagatcaa ttagaaggcg
     3001 tggtaacaga cgttactatc aacatcatga agtgttgcta attcgtcgca ttcatgaatt
     3061 gttatatgga caaggttttt ctattagtgg agcaagaacc cgtttatcta atgatggtgg
     3121 tcttgaatca ggtttgattg aagacccgtc tcaaacaatc acagcatgtt caactgaaga
     3181 actgttgtat atccgcaaag aaattaaggc gatcatctct ttattgcggt cttaagcgat
     3241 attcaatcgc taatctaaaa ctttgtttgc tataatttgg aaagagaaaa gacggggcgt
     3301 agcgcagcct ggtagcgtac ttgcatgggg tgcaagtggt cggaggttca aatcctctcg
     3361 ccccgaccaa ggcgttgtat aatattatta tcataatgaa ttaaatctca aatgcgcata
     3421 ttgcttagta acgatgatgg atactttgca cccggtcttg cgcatcttgc caatgctctc
     3481 tcgacaattg ctgaaattgt agttgttgca cctgaaagag atcgtagtgg ttcaagtaac
     3541 tctttaacgc ttgatcgccc acttaaattg cataaatcac ccaacggttt ttattttgtg
     3601 aacggtacac caaccgattg cgtccatttg gctgtaacgg gaatgctcga ttacttaccg
     3661 gatatgatag tgtcggggat caatgatggc gctaatatgg gtgatgatac aatttactcc
     3721 ggaacagttg ccgcagcgac tgaagggttc ttgttcggaa taccttccat cgctgtgtca
     3781 ttagtaaatc gatctgagtg taattttgct actgctgccc aagttgcatt agaaatggtc
     3841 aagcggttta tggagaaaag agttcatgtg cctgttttat taaatatcaa tgttcctgat
     3901 gtaccattga gtcgattagc cggcatggag gttactcgtt taggccgtcg ccataaagca
     3961 gagccagtta ttaaatctac aaacccgaga ggggaaacca tttattgggt tggagcagct
     4021 ggtattgcac aggatgcggg tgaagggact gatttttttg cagttcaaaa taataaaata
     4081 tcaattactc ccttgcaaat tgaccttaca cgatatgacc agataggcta tttaaaagat
     4141 tggttagatt tatcggattg agtgagtctt gttgaaataa attgattgaa aaggggtaga
     4201 gagtggatgc tcatcatcat ggcataggca tgacttcaca gcgtactcga gcgagaatgg
     4261 ttgaacgatt acgtgagcaa ggtattacag atgaaatggt acttgcagta atgaatacga
     4321 ttcctcgcca tctttttgta gaagaagcgt tggcatcaag agcttatgaa gatgtttcgt
     4381 taccgattaa ttttggacaa acgatttcga gcccttggat tgttgctaga atgactgagc
     4441 tgctccgagg aaatcataat ctaaataaag tgcttgaaat tggaacaggg tgtggttacc
     4501 aaacggctgt attagcgaag ttagccaagg aagtatattc gatagagcgt ataggcccac
     4561 tgctgacgag aacgcgtatc cgattgcgag agctaaggat aaccaatatt agccttaaac
     4621 atgcagatgg attgttaggt ttgcctgatg tagggccttt tgatgcaatt attatgacag
     4681 cagtaacgac tcaaatacct gaattactat tagaacaatt aactgtgggg gggaggatgg
     4741 tttacccaaa aggtatttca aaacaattct tgtgcgtaat tgaacatacg tcccaaggtt
     4801 ttattgaaac catacttgat gaagtaaagt ttgtgccaat tttatctggt gtagtaaata
     4861 gatagatgat agtaggggag ggttatctgg gatgttcagt caattgataa aggaaagaat
     4921 cgtgttcggt gcatcaacaa aaatgtgttt tggcctaaat gttaaccgat atattctggt
     4981 tactgatatt tatcaagtct tattactgtt actgatgtgt tgggtcgtta caggctgtgt
     5041 atcacctcac actgccccga ttgttgaatc tcagcaaaat ccaacccgtg caccatcccc
     5101 tgaagtggtt aaaccgagaa tacagaataa gcagctctac gtggttcaga gaggggatac
     5161 gttatacgga attgcactga aacatggcgt tgattataag gaattagttc aatggaatgg
     5221 aattgccgac cctaatacat tgaaagttgg tcaagaaatt gacttgctag tcccttcacg
     5281 ccaatcaaaa gttgctgaaa tagcgccaga agatcaatct gcgcaaccaa cattatttgc
     5341 gttgccgcag tcggcagctc cagtagaggt caattcaatt aagccactac agacagaacc
     5401 tgttagtccg tcacctggag caatggtcat tgcagagcct aagagtatta agctacctta
     5461 cgctgaacca tctgcggcta aaccagattt agaagtaagt caatctacta agaatcagcc
     5521 tgctgctatt ttgcctccgc ctaacaaaat agagaaacca agtgatgtag agaagcctac
     5581 aaaagctcaa ccagaaattg cgctacccaa aaataataac aacagtatca agtggagctg
     5641 gccaaccaat ggaaaagttt tgagcaaata tactgaaaga acaaaagggg ttagtatttc
     5701 tgggaataaa ggacaatcgg tattggcctc ggcggatggc actgttgttt atagtggaaa
     5761 tagtttacgc ggctatggaa atttaatcat tgtgaagcac aatgatattt atttaagtgc
     5821 atatgggcat aacagcaaaa ttttggtccg tgaaggagaa accgtgtcaa aggggcaaaa
     5881 aatagctgaa atgggcaaca ccgatagcga aacggttaag cttcatttcg agattagaga
     5941 acgaggtaag cccgttgacc cactcaaatt tttgtcaaat tagcacttag aatgattata
     6001 tgattgggag tcatcagccc ttacatttgg tatgagcctg cctagctaga gattacttct
     6061 gagagatgga aatagcgctt cttgccagat ttttagtttc ttttcatagg gtatggaagc
     6121 attagcgggg cttgttgagg gaagtctgat gaattgtttt ggacttccgc tgattgtttt
     6181 agcaacataa cgattaaagc aattttctgc tttacttcca ttaaaacata cctttgaaat
     6241 caggtggtgt tgctgcaaga agtgctgaag gttatttaca gtttgtgaat taggcaaaat
     6301 atttgaatca aggctaccaa ctcgcttaca tgatctcagg acatcccaga gtgcaatatg
     6361 agctgattta agtttttgga ccctcgtttc ataggatgaa tttaattcga actgaagcaa
     6421 ctcagccatt attttccaga atacattttg tttatgtgca taatattggc ctgccatcag
     6481 tgatgcattt cctggcatac ttcctaaaat taaaacgcta gcatggtggt ctgcgatggg
     6541 ttcaaaactg aaaatataag tcatttaagt attagcgtac taatgaatat ttcctggtag
     6601 tttcagcgct atcactggat ctgctagtta atgttcatta tttttatctg agtattcagg
     6661 aacccatttt gctaaagcca tccgaacctc ggtgtctgat aacgcgggct gttcgatgag
     6721 ccatgtaatg aatgaagtta gccatcgttc gtctactgga tgggacagcg taattcgaag
     6781 tttttcgtgc tgagttggta gggtgctctc attctcagtt attaattctt catgaagctt
     6841 ttcacctgat cgtaaacccg tataagtaat tttaatttct tcttcactga atccagatag
     6901 tcgaatcata tctttagcaa gatcaacgat tctaatagga ttgcccatat ccataacaaa
     6961 aatttctccc ccccctttta ttccacccat agaaccggct tgaagaacga gttgtgctgc
     7021 ttcttgaatt gacatgaaat agcgagaaat atcaggatga gtgactgtaa taggtccccc
     7081 tctcgcaatt tgctcacgaa acttaggtat tacgctcccc gtgctaccta aaacgttacc
     7141 aaaacgtaca atgacaaagc gggtgctagg tgaaaaaatg gtattggctt gcatactctc
     7201 ttgagagaga attgattgtt gcaatgcttg gcaaaccatc tcagctaatc gcttgctcgc
     7261 gcccattaca ttggtagggt taactgcttt atcagtcgat atgagtacaa atttagtgat
     7321 gtgatgtttg atcgctaatt tagctaaaac ataggtaccc agaacattat tttgtaatgc
     7381 ttgccaagta ttttcttttt ccattaatgg aacatgcttg taggcagcgg catggaaaat
     7441 aacagtgggt tgatactgtg caaaaagctg cgctaatctc gattcatctt tgacatcgcc
     7501 aatgacgaaa gccatcgcga tatcaggaaa tttgctacaa aattcttctt gaatcgagta
     7561 gagcgcaaat tcatttagct caaataaaac tagttgtttc ggtttgaagg tgatgatttg
     7621 tctgcaaagt tctgatccaa tcgagccgcc tgcacctgtt atgagtatgg ttttgtttga
     7681 taacagggta tgtaagcctt cggcatcgag gataacaggc tcacgaccga gtaaatcatc
     7741 aagttcaata tctcgaattt gcgaaacagt catttttcca ctgactaaat cagtgtaag
//
LOCUS       DAOQLJ010000110         7645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_3887_length_7645_cov_6.621476, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000110 DAOQLJ010000000
VERSION     DAOQLJ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3887_length_7645_cov_6.621476"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="PK522_08280"
     CDS             complement(<1..1547)
                     /locus_tag="PK522_08280"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNC41767.1"
                     /translation="MSEANALFLEASKYLKPEELKLLSRSYYFSQHAHQGQFRKTGDP
                     YISHPVAVAKILCSLRLDALTLASALLHDVVEDTDTSKAEISENFGETVAELVDGVSK
                     LDKIESQTQADAQAENFRKMLLAMAQDVRVILIKLADRLHNMRTLGVMRTDKKKRIAR
                     ETLEIYAPIAHRLGLNNIYQELQELGFQHFYPNRYKVLTKATKAARGNRREVVSKILE
                     AIKQKLLNAGIDAIVTGREKHLYSIYKKMSEKQLSFSEVLDIYAFRVLVKDTRSCYAA
                     LGALHGLYKPIPGKFKDYIAIPKPNGYQSLHNTLLGPYGLPLEIQIRTYEMHRIAETG
                     VASHWLYKSSDNEDNDLHLKTNQWLKSLLETLSDSSDSVEFLEHLKIDIFPGDVYVFT
                     PQGKILALPRGSTVVDFAYAIHTDVGNRCVAAKVNSENTPLRYKLKNGDRVEIITAPT
                     ANPNPGWLSYVNTGRARSSIRHFLRTIQYDESAELGERLLNQVLLSFNIIPETITPAQ
                     WDKLVKD"
     gene            complement(1630..1833)
                     /gene="rpoZ"
                     /locus_tag="PK522_08285"
     CDS             complement(1630..1833)
                     /gene="rpoZ"
                     /locus_tag="PK522_08285"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNC41768.1"
                     /translation="MARITVDDCLEIIPNRFDMTLAATVRARQISIGSTPLIENEKDK
                     PTVIALREIARGKINADILNTIR"
     gene            complement(1854..2459)
                     /gene="gmk"
                     /locus_tag="PK522_08290"
     CDS             complement(1854..2459)
                     /gene="gmk"
                     /locus_tag="PK522_08290"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNC41769.1"
                     /translation="MSSLIIISAPSGTGKTSIVSALTESDSQLSLSVSHTTRRPRPNE
                     VEGQDYYFVDRDIFQAMVNRGEFLESAEVYGNLYGTSHKWVKESLASGRDILMEIDYQ
                     GAQQVRKAFPQAISIFILPPSYAQLEERLKQRGQDDQKTISRRLANVREEVSHLNEFD
                     YVVVNNELQEAIKDVNCIIKAERLRIMRQLINQRTLVAQFY"
     gene            complement(2660..3358)
                     /locus_tag="PK522_08295"
     CDS             complement(2660..3358)
                     /locus_tag="PK522_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41770.1"
                     /translation="MQTNTDSTNSHEDDLKKLESDIREAIAHGGNVKEAVHKLTLNAM
                     QANRLDIDSLGRIASAVMQGIHEGASQKLALASEQSHIAKDQISHAVSGLDTALAQFV
                     GASKLALEEAAGKAKQFSREELSKTRADLESLESLFLETLQRTASTAKGLIADALNDL
                     LMHAKRNGTTIGEQLKDTLATFTHQLSSVSHSQIEASLQLAQTTADLLHKISSGILSG
                     ISEQTKRNDSENKQ"
     gene            complement(3404..4720)
                     /gene="serS"
                     /locus_tag="PK522_08300"
     CDS             complement(3404..4720)
                     /gene="serS"
                     /locus_tag="PK522_08300"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNC41771.1"
                     /translation="MLDIQQLRNELDLVAVKLAKRGYEFPVDQFRSLELKRKVIQTET
                     QELQAKRNAVSKQIGIAKQKGEAVTAMMAEIANLGDSLKQFENQLEQIQNELQKILLV
                     TPNLAHESVPEGRDDKNNLEIRRWGKPKLFDFEIKDHVTIGEQLGMLDFETASKLSGA
                     RFNVMKGGIARLHRALAQFMLDTHTLEHGYTEVYVPYLVNADCLRGTGQLPKFAQDLF
                     VAQSGAEIKSDSDLADQGIGKLHLIPTAEVPLTNMVSDKIIPCEQLPLKFVAHTPCFR
                     SEAGSYGKDTRGLIRQHQFDKVELVHIVHPHHSFEALEQLVHHAETILQKLELPYRVM
                     VLCTGDMGFSAAKTYDIEVWLPAQNTYREISSCSNCEAFQARRMQARFRNEKGKTELL
                     HTLNGSGLAVGRTLVALLENYQNADGSITIPSVLKKYMNNLDRITI"
     gene            complement(4830..5357)
                     /locus_tag="PK522_08305"
     CDS             complement(4830..5357)
                     /locus_tag="PK522_08305"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNC41772.1"
                     /translation="MPIKSLIRTIAHYPHEGIQYRDITTLLKDPIGLKTTIEEIAKRY
                     QLMKIDKVVGIESRGFIIGAPVAYQLEIGFVPIRKSGKLPAKTIGCEYDLEYGTDRIE
                     LHTDAIQPGERVLLIDDLIATGGTMEAAVKLIHELGGEVVECCFVVDLPEAGGRLRLE
                     KQGQKVFSLCQFEGS"
     gene            5645..6856
                     /gene="zapE"
                     /locus_tag="PK522_08310"
     CDS             5645..6856
                     /gene="zapE"
                     /locus_tag="PK522_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HNC41773.1"
                     /translation="MKQTTKQSEGPEAEYQALLQAGELQPDIAQRRAIHALERLHHEL
                     FAYSEVTNRGSQRSFLSWWPFRALTRNTNNLTAPKGVYLYGGVGRGKSMLMDVFYSVT
                     PTTQKKRVHFHEFMLEVHARLKAINEHSAQKRKRESRYLSGDNDPIPLIAQEIADETV
                     LLCFDELHVTDIADAMVLTRLFDKLFELGVVVVATSNRLPDDLYKNGLNRQRFLPFIE
                     QIKEKLHIIALDGPVDYRYNRLKGAQTYYFPINEDATNQLAITFFQLTDRRIEDRDKV
                     PSEELIVQGRKLFVPKAAKGVAVFSFKRLCANPLGTADYLAIARTYHTVIMVAIPQFS
                     QETEDQARRFVLFIDALYEYSVKFLCSAAVPVQLLYTGSEISFEFERTISRLMEMQSE
                     DYLTQGHGKAI"
     gene            6872..7204
                     /locus_tag="PK522_08315"
     CDS             6872..7204
                     /locus_tag="PK522_08315"
                     /inference="COORDINATES: protein motif:HMM:NF018784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HNC41774.1"
                     /translation="MRKIRTLVLVYFAILILSGCGTLTTVFQADSVTKRKLNQVETKC
                     ELVPRIYSGIAYDFCVLRGKPSQAALWLVPVPELIMVDIALSAALDTALLPYTIYEQI
                     QSGSIYIQ"
     gene            7313..7498
                     /locus_tag="PK522_08320"
     CDS             7313..7498
                     /locus_tag="PK522_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41775.1"
                     /translation="MTWSEARWVKKGDKSVFGYEKLTGLASNGLLMAVLATAVNCHDS
                     ELLARLERLPNSLPNTE"
BASE COUNT         2149 a         1482 c         1550 g         2464 t
ORIGIN      
        1 ctatctttta caagcttatc ccattgagct ggagtgatag tttctggaat gatgttaaac
       61 gatagtaata cttgatttaa gagacgctcg cccaattctg ctgattcatc atactgaatg
      121 gttctgagga aatggcgaat acttgaccgt gctcttccag tattaacata ggataaccac
      181 ccaggatttg gatttgccgt tggtgcggtt atgatttcaa ctctatcacc atttttcagt
      241 ttatagcgga gtggggtatt ttcgctattt acttttgcgg ccacgcagcg attcccaaca
      301 tcagtatgga ttgcatatgc gaagtcaact acagtagagc ctcgtggcaa tgccagtatc
      361 ttaccttgag gtgtaaaaac atatacatca cctggaaaaa tgtctatttt caagtgttca
      421 agaaattcaa ctgaatcaga ggaatcactg agtgtttcta gtaagctttt taaccattga
      481 ttggttttta aatgtaagtc attatcttca ttatcgctac ttttatataa ccaatgagat
      541 gcaacaccgg tttctgctat tcgatgcatc tcataggtac gtatctggat ttctagtggt
      601 aacccgtatg gacctagcag agtattatga agtgactgat atccattcgg ttttggaatg
      661 gcaatataat ctttaaattt tcctggtatt ggcttatata aaccatgtaa agcccctaaa
      721 gctgcgtaac atgatcgagt gtctttgact aatactcgaa aagcgtagat gtctagaact
      781 tctgagaagc ttagctgttt ttcagacatc ttcttgtaaa tactataaag atgtttctcc
      841 cgaccagtca cgattgcatc gataccagca ttgaggagtt tctgcttaat agcttctagt
      901 attttactaa ctacctcgcg acggtttcca cgtgcagcct tggttgcctt agttaatact
      961 ttatatcggt taggataaaa atgttgaaac ccaagttcct gtaattcctg gtagatatta
     1021 tttaatccga gtcgatgtgc aataggtgca taaatctcaa gtgtttctcg agcaatacgt
     1081 ttttttttat cggtccgcat tacaccgagg gtccgcatat tatgcaagcg atcagcaagc
     1141 ttgattaaaa tgacgcgtac gtcttgtgcc atagcaagca gcatcttgcg aaaattttct
     1201 gcttgagcat cggcctgagt ttgtgattcg attttatcga gcttagaaac cccatcaaca
     1261 agctctgcaa ctgtttcccc gaaattttcg ctaatctctg cttttgaagt atccgtgtct
     1321 tcaacgacat cgtgtagtag cgcagaagct agtgtcaatg catcaagtcg aagactacat
     1381 aaaattttag ctactgcgac aggatgggag atataaggat cccccgtttt acggaattga
     1441 ccttgatgag catgttgact gaaataataa gagcgactga gtagttttag ctcttcaggc
     1501 ttaaggtatt tagatgcttc taaaaataac gcattagcct cagacatttc cataggaact
     1561 taagcgctac gtttgacgtg ctgcaacgaa gtgtatttgg aaatgggtag tagaagttat
     1621 ttcaaattac tagcgaatag tattgagtat atcagcattg attttacccc ttgctatttc
     1681 gcgcaaggca ataacggtgg gtttatcctt ctcgttttca atcaacggag ttgatccgat
     1741 cgaaatttga cgggcccgca cagttgcagc cagtgtcata tcaaagcgat taggaattat
     1801 ttctaaacaa tcatcaacgg taatacgtgc catcagtttc tctcttcaaa aaattaataa
     1861 aattgggcga ctagcgtacg ctgattaata agttgtcgca ttatccttaa tcgttctgct
     1921 ttaatgatac agttaacatc cttaatagct tcttgtaatt cattattaac aacgacatag
     1981 tcgaattcat taaggtgact cacttcttca cgaacgttag caagacgtcg tgaaattgtt
     2041 ttttgatcat cttgccccct ttgttttaga cgttcttcaa gctgcgcata ggatgggggt
     2101 aaaataaaaa tactaattgc ttgaggaaat gctttgcgta cttgttgcgc tccctgataa
     2161 tcaatctcca tgagtatatc tcgtcctgaa gcgagggatt ccttaaccca cttatgagaa
     2221 gttccataca aatttccata tacttcagca ctttcaagaa actctccacg attcaccatc
     2281 gcttggaaaa tatctcgatc aacaaaatag taatcctgtc cttccacttc attaggtctt
     2341 ggtctgcgag tagtatgcga tacagataaa cttaactgac tatcagattc ggttagcgcg
     2401 ctgacgatac tagtttttcc tgtcccagat ggggcgctaa taataataag gctactcatg
     2461 ttggtgatta tattggatat tgcggtattc tggataatat aatgctaatt ataatgtctc
     2521 tatttgattt gatctgggtt ttatcggtaa gaaatatatc ttttataaaa gagagaagct
     2581 aacttagcga ttcgaataac cttttaagca gatttttcag taataaattc gattctatat
     2641 atctttttca aattaggtac tattgcttat tttctgaatc gtttcgtttc gtttgctccg
     2701 aaattccaga caaaattcca cttgaaattt tatgaagtag atctgctgtt gtttgggcga
     2761 gttgtaaact agcttcaatt tgagagtgac ttactgaaga caactgatgt gtgaacgttg
     2821 ccaaggtatc tttgagttgc tcaccgatcg ttgttccatt tctttttgca tgcataagga
     2881 gatcattaag ggcatcagcg atcagtcctt ttgctgtcga cgcagttcgt tgcagagtct
     2941 cgagaaataa gctttctaga ctttctaaat cagcacgtgt tttagagagt tcctctcgtg
     3001 aaaattgctt ggcttttcca gctgcttctt ccaatgctaa cttggaagca ccgacaaatt
     3061 gggccaaagc agtgtcaagg cctgagactg catgactaat ttggtctttt gcaatatgtg
     3121 actgttcaga agcaagtgct aatttttggc tggcaccttc atggatacct tgcataacgg
     3181 cactagcaat tcgtccgagt gaatctatat caagtcggtt agcttgcatc gcgttgagtg
     3241 taagtttatg gaccgcttct tttacatttc caccatgggc gatagcctct cgaatatctg
     3301 actcaagttt ttttaaatca tcttcatggg aattggtgct atcagtattg gtttgcattt
     3361 tgtttctcct gaattaaatt aaaaattatt ggatgtaccc gtgttagatt gtgattcgat
     3421 caaggttatt catatatttc tttaaaacag atggaatggt aatactacca tcagcatttt
     3481 ggtaattttc caaaagagcg acaagcgttc gaccgactgc tagacctgat ccatttaacg
     3541 tatgtagtaa ttctgttttt cctttttcat tgcggaatct agcctgcatg cgtcgagctt
     3601 ggaaagcttc acaattactg caagaagaaa tttcacgata agtattttgt gcaggtagcc
     3661 atacctcgat atcataggtt tttgctgctg agaatcccat atctcccgta cagagcacca
     3721 tgacgcgata aggtaattca agtttttgca aaatggtttc agcatgatgg accaactgct
     3781 ctagcgcctc aaaggaatgg tgtggatgga caatgtggac tagctccacc ttatcgaact
     3841 gatgttggcg aattaaccct cgtgtatctt taccataact ccctgcttct gagcggaagc
     3901 agggagtgtg tgcgacaaat tttagcggaa gttgctcaca aggaatgatt ttatcactga
     3961 ccatattcgt taaaggtact tctgctgtgg ggattaggtg caatttacct ataccttgat
     4021 cagcgagatc tgagtcagat ttaatttctg cacctgactg ggcaacaaat aagtcttgtg
     4081 caaatttggg caactgtcct gtgccgcgca aacaatcggc attcactaaa taaggcacat
     4141 agacttcagt gtaaccatgc tcaagcgtgt gggtatctaa cataaactgg gctagcgccc
     4201 gatgtaagcg agcaataccg cctttcatga cgttaaaacg agcgccactt aatttagagg
     4261 ctgtttcgaa atcaagcata cctaattgtt caccaatcgt gacatgatct ttaatctcga
     4321 aatcaaatag tttaggtttt ccccagcgtc ttatctcaag attattttta tcatctctgc
     4381 cttcaggtac gctttcatgt gccaaattag gggttaccaa taatattttt tgcagttcat
     4441 tttggatctg ttcaagttga ttctcgaatt gtttaaggct atcacctaaa ttggcgatct
     4501 ctgccatcat tgctgtgact gcttcccctt tttgcttggc aatcccgatc tgctttgaga
     4561 ctgcattcct ttttgcttgc agctcctgtg tctcggtttg aattacttta cgttttagtt
     4621 caagtgacct gaattggtcg actggaaatt catagccacg tttggctaat tttactgcca
     4681 caaggtcaag ttcgttacga agttgctgga tatctaacat ccttgatggg taccttatta
     4741 atgatgagtg ataaagcgtt agtttttttt acttaatgaa tcgttatcaa atgaaaaatt
     4801 acaactaaca ttaataaatc atttctcagt tagctgcctt caaattggca aagtgaaaac
     4861 actttttgtc cctgtttttc taagcgtaac cttcctcctg cttcgggtag atcaactaca
     4921 aaacagcatt cgacaacttc tccgcccaat tcatgaatta acttaacagc tgcctccatg
     4981 gttccacccg ttgcaattaa atcatctatc aacaacacgc gttcccccgg ctgtattgca
     5041 tcggtatgaa gttcgatacg atctgttcca tattctaaat catattcgca tccaatagtc
     5101 ttggctggca atttgccaga tttccgaatg ggtacaaaac caatttctag ttgataagcg
     5161 actggagcac caatgataaa tcccctggat tcgattccaa ccactttgtc gattttcatc
     5221 aactgatacc gttttgcaat ttcctctatg gtagttttta acccaatagg atcctttagc
     5281 aaggttgtaa tatctcgata ttggatccct tcatgtggat aatgggcaat cgtccgaatt
     5341 agggatttta tgggcatata aagttaataa aataatattt gcttgcgaaa tagctaatca
     5401 ttttatcgtt ttttaaactt gacagagaag atgattctgc gatttataaa aaatctgttg
     5461 atggtaaaaa attcagtttg ttattagata gtagcatcag gaaacattac gatcatttat
     5521 cagtgaatca atccatgtga tgtttaaagc agagctaacc gggaattgat ctactgttag
     5581 ctttttatca gtatgattca gatcattata tttgaacaat tcagatagcc tatatcgaag
     5641 taccatgaaa caaactacta aacagtccga agggcccgaa gcggaatatc aggcattatt
     5701 gcaggcaggt gaattacagc ctgatatagc acagcgtcga gcgattcatg cattagagcg
     5761 attgcaccat gaattatttg cttattctga agtaacgaat agagggagcc aacgatcttt
     5821 tttgtcatgg tggcctttta gagcactaac aagaaatact aacaatttga ctgctcccaa
     5881 aggagtttat ctttatggtg gcgttggtcg tggaaaatca atgctgatgg acgtgtttta
     5941 ctcagttact ccgaccactc agaagaaacg cgtccatttt catgaattta tgctggaagt
     6001 tcatgcaaga ctgaaagcaa tcaatgagca ttctgctcag aagcgcaaac gggaatcacg
     6061 gtacttgtca ggtgataatg atccgattcc actgattgcg caagaaatcg ctgatgagac
     6121 tgttttgttg tgttttgatg aattacacgt gaccgatatt gccgatgcaa tggtgttgac
     6181 gcgcttattc gataagttat tcgaattggg agttgttgtg gtagcgactt ctaaccgact
     6241 tcctgatgat ctctataaaa atgggttgaa tcgacaacgt tttcttccat ttattgagca
     6301 aatcaaagaa aagctgcata ttattgcatt agatggcccg gttgattatc gctataatcg
     6361 tttgaaaggc gctcagactt actattttcc gattaacgag gatgcaacta accaattagc
     6421 cattactttt tttcagttaa ctgatcggcg tattgaagat cgagataaag taccgagtga
     6481 agaactcatt gtgcaaggaa gaaaactgtt tgttcctaaa gcggctaagg gggttgcggt
     6541 tttctcgttc aagcgattat gtgctaaccc tttaggtacc gctgattatt tagcgattgc
     6601 tagaacgtat catacggtaa taatggtcgc aataccgcaa tttagtcagg aaactgaaga
     6661 ccaagccagg cggtttgttt tatttatcga tgcgttgtat gagtatagtg ttaaatttct
     6721 ctgctcggca gcagtgccgg ttcaattgct ttataccggt agtgaaatta gttttgaatt
     6781 tgagcgtacg atttcacggc tgatggaaat gcagtcagaa gattatttaa cacagggcca
     6841 tggcaaagcc atttaaaatt aatattaaag tatgagaaaa attcgaacgc tggttctagt
     6901 gtactttgct attctgattc tctcgggatg cggcaccctg actactgttt tccaagcaga
     6961 tagcgttaca aaacgtaaac tgaatcaagt tgaaactaaa tgtgaattgg tgcccagaat
     7021 ttatagtgga atagcgtacg atttttgtgt attgcgtggg aagccatcgc aagctgcgct
     7081 gtggttagtt ccggtacctg agttaataat ggtcgatatc gccctatcag cagcattaga
     7141 cacagctcta ttaccttata cgatttatga gcaaatccaa agtggtagta tttatattca
     7201 gtgaaagtcc tattacctga attctgcgat catttctttg agtagttaac aatgtcagga
     7261 aaatttcttg tcaaccttgt ctcgatgaaa attgagtaaa cttagtattt tagtgacctg
     7321 gtcagaagca cgctgggtta aaaaaggcga caaatctgta ttcggttatg agaagcttac
     7381 gggattagct agcaatgggt tgttgatggc agtattagct actgccgtca attgtcatga
     7441 tagcgagttg cttgctcgac ttgaaagact tcccaactct ttgccgaaca ctgaatgact
     7501 ccgcaaatat aggagatagt gcgcctactc taccggaaat agccgattta gatggactaa
     7561 ttacaagctt gttcatggaa aaattactaa aaagcccaat tcctcaataa attagcccgg
     7621 aattttacta gaatgcaaag cttgc
//
LOCUS       DAOQLJ010000111         7461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_4030_length_7461_cov_5.565893, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000111 DAOQLJ010000000
VERSION     DAOQLJ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7461
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4030_length_7461_cov_5.565893"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PK522_08325"
     CDS             <1..617
                     /locus_tag="PK522_08325"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HNC41776.1"
                     /translation="PQADPAAREAWKKKLPERLTEEVAAFGTPRPLRLMFQDEARFGR
                     ISDVRHCWDKKPNRPVVRAMLTQQYTYAYGAVSPLDGRFDSLILPWVNGDCMQLFVDE
                     ISARYPHENIVMVMDGAGWHKSQTMKLAENMRILFLPPYSPELNPQEHVWDELREKFF
                     HNRAFDSLDALEIHLESALKSFESNQETMRSITGWDWIINAVSK"
     gene            796..1164
                     /locus_tag="PK522_08330"
     CDS             796..1164
                     /locus_tag="PK522_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNC41777.1"
                     /translation="MLPTTNRSFPMNKTELIDAIAARAQTTKTQTATILNGFMETVQK
                     TLAKGDTIQLVGFGTFLVAQRASREGRNPATGKTMTIPAKKVVKFRPGKALADAAAAT
                     KAPRKAQPAAKPKAAAAKRK"
     gene            1490..2284
                     /locus_tag="PK522_08335"
     CDS             1490..2284
                     /locus_tag="PK522_08335"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HNC41778.1"
                     /translation="MDQNTFNLDKNQLVQHAFDQMEKHVKGADYTPRQKLALTCRILF
                     DNGHDSGLAGQITARSDQSGAFLTQRLGLGFDEISEANLLLVDEHLQVISGEGMPNPA
                     NRFHAWVYRARPDVNCIIHTHAIHTAALSMLEVPLQISHMDNCVLYDDVAFLPKWPGV
                     PVGNDEGELIAAALGNKRALLLAHHGLLVASSSIEEACLLALAFERTARMQLLATSVG
                     NIQPIDPVLGREAHDWILRAQRNAAAFAYYARRSLRLPHNQDCLAE"
     gene            2287..5937
                     /locus_tag="PK522_08340"
     CDS             2287..5937
                     /locus_tag="PK522_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC41779.1"
                     /translation="MFHFTSLTQRFAVWFAVVALLPILLIGYSLLQTFEAEIKKAVIQ
                     QVSSIADKKIEQIDAYFNERMLDASVIQAADTTHQAIKEYTAAFKQFGVNSEAYRDLD
                     AIYRNHFERFVKHADYYDVFLISTDGWIVFTYSRESDFATSLLTGPYRDTGLGAVARR
                     ALREQKGSISDFEYYEPSKGAIAAFIAEPIIIEGEFSGVFALQIYSDPVFKVLTNNVG
                     LGQSGETILTRLESENTALVMAPLKKEPDAALKHKILLDDSRFSTGIRRSLAGQDGGG
                     LTIDYLGKEVVAAWRYLPLMKWGMVVHIDVSEAFASVYRVRAVGLVILFLTFIVATSG
                     ALLFNRRVVTPLKNLNEGAQAIASGNLNQRVPNEGWDEMEKLANAFNTMTERLNTSYR
                     GLEKRVAERTAELETANAELAIKEEETRSVVEHMVDCVVTTDEKGVMLSVNPVMEKLF
                     GYTPDEAIGQNVAILVPEPDRSQHEFYMERYCNTGHGQTYVGRPYLPGTHGIGLGREV
                     EGVHKNGQLIPVYLAVSEYFVGEKRHFTGVMRDIREHVKIMKDLEHARLEAEQANQAK
                     SAFLATMSHEIRTPMNGVIGMIEVLQQSSLSSYQMEMTNLIRESAFALLEIIEDILDF
                     SKIEAGKLEIEFAPIPLATVIEKACSMLEHLAANKGIELTLFIDPAIPDEVLGDALRL
                     RQIVVNLINNAIKFSSGQGRPGKVSTQVLLIEADHDQVMISIQVTDNGIGMNEETRER
                     LFIPFTQGDTSTTRRFGGTGLGLTITHHLVKLMGGEVTVQSKEGEGSTFIVHIPFKPL
                     PSETTTHQKQIDLAGLSCIVYGPDEGLSNDLVAYLKYAGANVERVTDLSAARKRIKTL
                     PAGQWLLVIDAGHDESSVDELRLAFRTRLNLDSPDKPHLVVVKRGRRHRGRIEKGEQV
                     TLDGNILYRFSFLHAVAVAAGRAKLEEDNLPLEGSSTRIIPPSREEALQQGRLILVAE
                     DNEINQKVIRQQLALLGHVADVVQDGRDALRHWESGDYAMILTDLHMPEMDGYQLTQA
                     IRAAKKGAIQIPIVALTANALKGEADRCRAIGMDDYLSKPVQLEQLKQTLQKWMPKHS
                     DDSKVPEQAKQQVINQQATPVDIRILQALVGDDPAITQEFLGDFKTNAQVISDQLHMA
                     HQQHQLDQVKAAAHKLKSSSRSVGAQVLGEWCEKIEQAARNHDEQSLTSLLPQFDAEL
                     QAVKHYLETMNA"
     gene            5959..7128
                     /gene="sucC"
                     /locus_tag="PK522_08345"
     CDS             5959..7128
                     /gene="sucC"
                     /locus_tag="PK522_08345"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNC41780.1"
                     /translation="MKIHEYQAKEILRQFGVTTPNGIACFSVNEAISAAQSLGGNRWV
                     VKAQIYAGGRGKGGGVKLARSLDEVRQFAEGMLNKPLITHQTGPQGQIVHRLYIEQGV
                     DIQHEYYLGMVIDRARQRVCLMASAEGGMDIEEVAAKTPEKIHKILIDPVAGLKDEDI
                     THIAGKISLPSTSLDATRVLLKGLYQAFDQTDASLLEINPLILTTSGQVIALDAKMNF
                     DDNALFRHPEIVALRDTDEEDPAEMEAAKHDLSYIALDGNIGCLVNGAGLAMATMDII
                     KLYGGHPANFLDVGGGATAEKVTEAFKIMLRNPKLQAILVNIFGGIMKCDVIAQGVTT
                     AAREVKLTVPLVVRLEGTNVDLGKKILAESGLPIISASNMADAAEKVVNAIQKGA"
     gene            7131..>7461
                     /locus_tag="PK522_08350"
     CDS             7131..>7461
                     /locus_tag="PK522_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNC41781.1"
                     /translation="MSILINRDSRVITQGITGKTGQFHTQLCREYANGRQCFVAGVNP
                     KKPGEQFEGIPIFATVKEAKQTTGATVSVIYVPPPFAAAAIDEAVEADLDLVICITEG
                     IPVRDMLR"
BASE COUNT         2078 a         1517 c         1887 g         1979 t
ORIGIN      
        1 atccacaagc ggacccagca gcgcgcgagg catggaaaaa aaaactcccc gagcgactaa
       61 ccgaggaagt ggcggctttt gggaccccac gcccgctgcg cctaatgttt caggacgaag
      121 cacgtttcgg tcgcatcagc gatgtgcgtc actgctggga caagaagcct aatcgccccg
      181 tagtacgtgc catgctgacg caacaataca cttacgccta tggcgcggtc agcccactgg
      241 atggacgatt tgattccttg atcctgccct gggtgaacgg ggactgcatg caactcttcg
      301 tggatgaaat ctcggcacgt tacccgcatg aaaatatcgt tatggtaatg gacggcgcag
      361 gctggcacaa aagccagacc atgaaattgg cagagaatat gcgaatccta tttctaccac
      421 catactcgcc agaactcaat ccacaggaac atgtgtggga tgaattacgg gaaaaattct
      481 ttcataaccg tgctttcgat agtctagatg cgcttgagat acatctggaa agcgcactaa
      541 aaagctttga atcaaaccag gagactatgc gcagcattac cggatgggat tggataatta
      601 atgctgtttc taaatagaat tggaataagt ccgattggaa gcctcaatga ctagagaaat
      661 ctggggtaaa aaccccagtt tcggtaattg taaggaaaaa agtgtcggaa aagcgacaaa
      721 caaagcaaaa ttttgtttaa aaaccccata aacatattgc ttttttgcga aatatcgggc
      781 gaaaatacca acatcatttt accaaccaca aataggagct tcccaatgaa caaaacggaa
      841 ttgattgatg caattgcagc tcgtgcacaa acaaccaaaa cacaaactgc aacgattctg
      901 aatgggttta tggaaaccgt acaaaaaacc ctagcaaaag gtgatacgat tcagctagtc
      961 ggctttggta cctttctagt tgcgcaacgt gcgagtcgtg aaggccgtaa tcctgcaaca
     1021 ggcaaaacca tgacaattcc agctaagaaa gtagtgaaat ttagaccagg gaaagccctt
     1081 gctgacgcag cggcagcaac taaagcgcct cgtaaagctc aacctgctgc taaacctaaa
     1141 gctgctgctg ccaaacgtaa ataatcccct tcttcaaaat aacgatcttg agattgcgta
     1201 tttgtgatct tgagagcttg ggaaaggcct acttgcaagc tgtatcagca ttgacggata
     1261 tcgcttaata ctatcaaact cgtaggcctt atttttctac ttactttcag tgttttgtaa
     1321 gcaatttttt gctgagtaca tcttcttcat aatactttca cacaagcttt agcttttctt
     1381 catgctttgc gggcattatt ccattgaatg gataatttat ttgctaagca cgatgtgatt
     1441 tgctactttg attttaaatt caattcttat tttgtcagag agcataatca tggatcaaaa
     1501 taccttcaac ttagataaga atcagctagt acagcatgca ttcgatcaaa tggaaaagca
     1561 tgttaagggt gcagattata cgccacggca gaaacttgcc ttaacctgcc gcatcttatt
     1621 tgataatggc catgattctg ggttggctgg tcaaattacg gcgcgatccg atcaatcagg
     1681 cgcatttcta acacaaagat taggcttggg ttttgatgaa atcagcgagg ctaatctttt
     1741 actggtcgat gagcatttgc aagtaataag cggtgaggga atgcctaatc cggctaatcg
     1801 ttttcatgct tgggtttatc gagcccgccc tgacgtaaat tgtattattc atacgcatgc
     1861 gatccataca gctgcgctga gtatgctaga agtaccattg caaatctctc atatggacaa
     1921 ctgtgtgtta tacgatgatg tagcattcct gccgaaatgg ccgggtgttc cagtcggtaa
     1981 tgacgaaggt gaattgatcg cagcagcgtt agggaataaa agagcgttgc tactcgcgca
     2041 tcatgggttg ttagtcgcgt ctagtagtat cgaagaggct tgtttattgg cgcttgcttt
     2101 tgagcggacc gcacggatgc aattactggc cacatcagtc ggtaacattc aacccatcga
     2161 tcctgtgtta ggaagagaag ctcatgactg gattttacgt gcacagcgca acgctgctgc
     2221 atttgcttat tatgcgcgac gaagtctgag attgccccat aaccaggatt gcttggcaga
     2281 gtaatcttgt ttcatttcac ctcccttaca cagcgatttg ctgtatggtt tgcggttgtc
     2341 gcgttacttc caatcttgct gattgggtat agcctgttgc aaacgtttga ggctgaaatc
     2401 aagaaggctg tcattcaaca agtttcttca atcgcagata aaaaaatcga acaaatcgat
     2461 gcctatttca atgaacgtat gcttgacgca agtgtgatcc aggccgccga tacgacgcat
     2521 caagcgatca aagaatacac ggctgctttc aagcaattcg gggtaaattc tgaggcatac
     2581 cgagatctag acgcgatcta tcgcaatcac tttgagcgtt ttgtaaagca tgcagactac
     2641 tacgatgttt tcctgatttc taccgatggg tggattgtct ttacctatag tcgcgaatct
     2701 gattttgcga cgagtctact gacaggaccc tatcgtgata ccggactggg cgccgttgct
     2761 agacgcgctt tacgcgaaca aaaagggagt atttctgatt ttgagtatta tgagccttcc
     2821 aaaggcgcca ttgccgcatt tattgcggaa ccgatcatta tcgaaggtga atttagtggt
     2881 gtttttgccc ttcagatcta cagtgatcct gtatttaagg ttttaaccaa taatgtaggc
     2941 ttgggtcaaa gtggtgagac gattttgaca aggttagaga gtgaaaatac agccttggtc
     3001 atggcaccat tgaagaaaga gcccgatgct gccttaaaac ataaaatttt attggatgat
     3061 tcgagattct caaccggtat tcgacgcagc ttggctgggc aagatggggg tggattgact
     3121 attgattatc tgggcaagga ggttgttgct gcctggcgtt atctcccact tatgaaatgg
     3181 ggaatggtcg ttcacatcga tgtcagcgaa gcatttgctt cggtttatcg tgtgcgtgct
     3241 gtcggtcttg tgattctgtt tttgactttt atcgtggcga catccggggc gctattattc
     3301 aatcgccgcg tcgtgacacc gctcaaaaat ctgaatgagg gcgctcaagc gattgcatcc
     3361 ggaaatttga accagcgcgt acccaatgaa ggctgggacg agatggaaaa gcttgcaaac
     3421 gcatttaaca ccatgacgga acgtttgaat accagctatc gggggttaga aaagcgagta
     3481 gcggaacgga ctgctgaact tgaaactgca aatgctgaat tggcgattaa agaagaagaa
     3541 acgcgttcag tggtcgaaca tatggtggac tgcgtcgtta caaccgatga aaagggagtg
     3601 atgctttcag ttaatcccgt gatggaaaaa ctgtttggct atactcctga tgaggcgatt
     3661 gggcaaaatg tagcgattct tgtaccggaa ccagatcgta gtcagcatga attttatatg
     3721 gagcgctact gtaatacggg gcatgggcaa acctatgtgg ggcgacctta tttgccagga
     3781 acacatggta ttggtttggg gcgagaggtg gaaggcgttc ataaaaatgg ccaattgatc
     3841 ccggtctatc ttgcagtcag tgagtatttt gtaggggaga agcgtcattt tactggagta
     3901 atgcgggata ttcgtgagca tgtaaagatc atgaaagatc ttgagcatgc acgactcgag
     3961 gcggagcagg caaatcaagc caaatcagcc tttctggcga ccatgagtca tgagatccgg
     4021 acgcctatga atggtgttat tggtatgatc gaagtgttgc agcaatccag tctaagtagt
     4081 tatcaaatgg agatgacgaa tttaattcgt gaatctgctt ttgctttgtt ggaaatcatt
     4141 gaggacattc ttgatttctc aaaaattgaa gcaggtaagc ttgaaattga atttgcaccg
     4201 attcccctgg ccactgtgat tgagaaagct tgcagcatgc tcgagcatct ggctgccaat
     4261 aaagggattg aattaacctt attcattgat cctgccattc ccgatgaagt gctcggagat
     4321 gcgttgcgct tgcgtcagat cgtcgtcaat ctcattaata atgctattaa attctcaagt
     4381 ggccagggac gacccggtaa agtttctacc caagtgttat tgatcgaagc agatcatgat
     4441 caagtaatga tttcgattca ggttacggac aatgggattg gtatgaatga agaaaccagg
     4501 gaacgattat tcatcccttt tactcagggc gatacttcga ccacacgacg tttcggtggg
     4561 actggcctgg gtctcacgat aacgcatcat ctcgtgaaat taatgggagg agaagttaca
     4621 gtccagagta aagagggcga ggggtctacg tttattgttc atattccttt taaaccgctg
     4681 ccttcagaaa cgactacgca ccagaagcag atcgatcttg caggtctttc ctgtatcgtg
     4741 tatgggcctg atgaaggatt gagcaatgat ctcgtcgcct atttgaaata tgcaggtgca
     4801 aatgttgagc gggttactga tctatctgca gcacggaaac gaatcaaaac attaccggct
     4861 ggacaatggt tactggtcat tgatgcgggt catgatgagt catcggttga tgagttacgg
     4921 cttgcatttc gtacccgttt gaatttagat tcacctgata agccgcattt ggtagtcgtt
     4981 aaacgcgggc ggcgccatcg ggggcggatt gagaagggtg aacaagtaac gcttgacggt
     5041 aatattttat atcgtttttc ttttttgcat gctgttgcgg ttgccgcagg gcgagcaaaa
     5101 cttgaggaag ataatttacc cttagaaggg tcatcgaccc gaattattcc tccttctcgt
     5161 gaagaggcat tgcagcaggg tagattgata ttggttgcag aagataatga aattaaccaa
     5221 aaagtaattc ggcaacaact tgcgctactt gggcatgttg ctgatgtagt gcaagatggt
     5281 cgtgatgcat tgcggcattg ggaaagcggt gattatgcta tgatactaac ggacctgcat
     5341 atgccggaaa tggatggtta tcagttgaca caagctattc gtgctgcaaa aaagggagcg
     5401 attcagattc caatcgttgc attgaccgcg aatgcgctta agggagaggc tgaccggtgc
     5461 cgagcaatcg gtatggatga ttatttgagt aaaccggtac agttagagca gcttaagcaa
     5521 acactacaaa agtggatgcc taagcacagt gacgattcca aagtgcctga gcaagctaag
     5581 cagcaggtaa ttaaccagca agcaacgcct gttgatattc ggatattgca agcattagtc
     5641 ggggatgatc cagcgattac tcaggaattt ttaggtgatt ttaagacaaa cgcacaggta
     5701 attagtgatc aacttcatat ggcgcatcaa caacatcaat tggatcaggt aaaggccgct
     5761 gcacacaagc taaaatcctc gtcgcgatca gtaggggccc aagtgctggg cgagtggtgc
     5821 gaaaagattg aacaggctgc taggaatcat gatgagcaga gcctcaccag cctgttgccg
     5881 cagtttgatg ctgaactgca agcggttaaa cactatcttg aaacaatgaa cgcataatcc
     5941 tttgaaagga gttagtcgat gaaaattcat gaataccagg ccaaagaaat tctacgtcaa
     6001 tttggggtaa ctacgcctaa tggtattgcc tgtttcagcg tgaatgaagc tatctctgct
     6061 gcgcagtcat tgggcggaaa tcgatgggtg gtgaaagcac aaatctatgc cggtggacgg
     6121 ggaaaagggg gcggggttaa gttagctcgt tcactcgatg aagttaggca gtttgcagaa
     6181 ggcatgctca ataagccact gataactcat cagactggcc cacaagggca aatcgtgcat
     6241 cggttgtata tcgaacaggg tgtagatatt cagcatgagt attatttggg tatggtgatc
     6301 gatcgtgccc gtcaacgcgt ttgcttgatg gcgagtgctg aaggtgggat ggatatcgag
     6361 gaggtcgcag ccaaaacacc tgaaaaaatc cataagattt tgattgatcc tgtggctgga
     6421 ttgaaggatg aggatatcac ccatattgca gggaaaatta gtttgccgag taccagcctt
     6481 gatgcaacca gagtcctgct aaaagggctt tatcaggcgt ttgatcaaac cgatgcttcg
     6541 ttattggaaa tcaacccatt aattctgacg acttcaggtc aggtaatagc gctcgatgca
     6601 aaaatgaatt ttgatgataa cgcattattt cgtcaccctg agattgtagc gttgcgcgat
     6661 acggatgaag aagatcctgc cgaaatggaa gcggccaagc atgacctctc ctatatcgct
     6721 ttggacggaa acattggttg cttagtaaac ggtgctggtt tagcgatggc gaccatggat
     6781 atcatcaaat tatatggagg acatccagcc aatttcttgg atgttggtgg tggtgcaacg
     6841 gctgagaaag tcacggaagc gttcaagatc atgctgcgta atcccaagtt gcaagcgata
     6901 ttagtcaata tctttggcgg aatcatgaaa tgtgatgtga tcgcccaagg ggttactacc
     6961 gcagcccgcg aagtcaaatt aacggtaccg ttggtggttc ggcttgaagg tacgaatgtt
     7021 gatcttggta agaaaattct cgccgaatcg ggcttaccga tcatttctgc cagtaatatg
     7081 gccgatgcag ccgagaaagt ggttaacgca atacagaaag gagcctagcc atgtcgatac
     7141 tgatcaatcg tgacagtcga gtgattacac agggcattac cggaaaaacc gggcagtttc
     7201 atacgcagct atgccgtgaa tatgctaatg gacgtcaatg ctttgtcgct ggggtaaatc
     7261 ccaaaaagcc aggcgagcag tttgaaggca ttcctatttt tgctacggtc aaagaagcaa
     7321 agcaaacaac tggtgctacg gtctcggtga tttatgtgcc accacctttt gcggctgcag
     7381 ccattgatga agccgttgag gccgatttag atttggtgat ctgtattact gaaggtattc
     7441 cggtgcgtga tatgctgaga a
//
LOCUS       DAOQLJ010000112         7364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_4115_length_7364_cov_5.621973, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000112 DAOQLJ010000000
VERSION     DAOQLJ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4115_length_7364_cov_5.621973"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PK522_08355"
     CDS             <1..606
                     /locus_tag="PK522_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNC41782.1"
                     /translation="KTSGEGDIRKNLVAEGNLIDCIVNLPAKLFLNTQIPAALWFLNR
                     NRAGTTVGVNDVRANNVGANNHLPLQHDHPHKNEILFIDARNLGHLINRRTRELSHDD
                     IHKIASTYHAWRNAAVVPAQAGIQPYEDIKGFCASVPISRVAELDYVLTPGRYVGLPD
                     EEDDFNFPERFAALRAEFEAQLREEAVLNQAIVESLARVKV"
     gene            603..1766
                     /locus_tag="PK522_08360"
     CDS             603..1766
                     /locus_tag="PK522_08360"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNC41783.1"
                     /translation="MSKWQECRLGEVAEIQTGPFGSQLKNSQYVTGGTPVITVEHIDN
                     FRIIDFDYPSVTYEDKKRLSKYLLNQGDIIFTRVGSVDLSAYVQPHQQDWMFSSRMLR
                     VRSDQRINSRFLSYFFQQNSFREYILSIAVGATMPSINTEILKSVPISYPSLPEQKAI
                     SSVLSSLDDKIDLLHRQNKTLEAMAETLFRQWFVEEDSGFREGCLADVAINVKEAIKA
                     SDLKSESKYIGLEHIDQRNIALKHYGFGSEVSSNKYAFQRHDILFGKLRPYFHKVCFA
                     PFSGICSTDILVIRPIKSELFAFCLFAFFQDEVVEYANLSSGGTRMPRTDWTTLKNYP
                     IAIPDSYSIERFNNAVSSSLKKISSNLEQIRTLETLRDTLLPKLMSGEVRVTV"
     gene            1763..2770
                     /gene="rhuM"
                     /locus_tag="PK522_08365"
     CDS             1763..2770
                     /gene="rhuM"
                     /locus_tag="PK522_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HNC41784.1"
                     /translation="MIEPIDNIVIYQPDDGLLGVEVRLRDETAWLSQAQIAALFDKDS
                     DTIGLHLKNIYQSGELIESATTEKSSVVRQEGGRQVRRNIKVYNLDAIISVGYRVNSK
                     KGTQFRIWASNILKRYLVQGYALNEQKLQMQQQKLTDLKQAIALSSRLLRQKDLTVSE
                     SQGILSILEKYSHALTVLDDYDHQRLQVTGIQQTGQSRISYDEAIQQIRLWREQEKLG
                     GLFGNEKDDSFKSSLETIYQTFGGTELYPSVEEKAANLLYFIVKNHSFSDGNKRIAAA
                     LFVWFLARHDYLLNADGEKRIADNALVAFTLLIAESKPEEKDTMVKVKVIINLINGNN
                     P"
     gene            2819..3421
                     /locus_tag="PK522_08370"
     CDS             2819..3421
                     /locus_tag="PK522_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41785.1"
                     /translation="MTDNPTSHRRKSIRLQGYDYSQAGLYFITVCTHNRVPLFGEIVD
                     GVMALNTAGQIVEKCWCTIPEHFPQVTVDEFVVMPNHVHGIITIGTTVGVNHVGANDV
                     GVNDVGANDYLPLPSNETSTQSNETPRSSQHGTSRTIGSMVRGFKIGVTRWFRANTDI
                     HKVWQRNYYEHIIRNEDSYLKIADYIQTNPQRWEIDTYYV"
     gene            3502..3696
                     /locus_tag="PK522_08375"
     CDS             3502..3696
                     /locus_tag="PK522_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNC41786.1"
                     /translation="MRTTIVLDDELLEKAQALTHVQEKSALVKEALKALIERESAKRL
                     ANLGGSEPQLEAIPRRQTSE"
     gene            3705..4079
                     /locus_tag="PK522_08380"
     CDS             3705..4079
                     /locus_tag="PK522_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC41787.1"
                     /translation="MILVDTSVWINHLRNNDPYLVRLLTENSVLGHPFVRSELALGNL
                     RQREIILAALDNLPQAPVAFTDEVNYFIEKHALYGLGIGLIDVHLLASTLLSGNTRLW
                     TQDKRLQAAALRLNLAALVHHV"
     gene            4072..7299
                     /locus_tag="PK522_08385"
     CDS             4072..7299
                     /locus_tag="PK522_08385"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNC41788.1"
                     /translation="MSKLTESAIETLAIQLFEQLGYSAIHAPDISPDGDHPERTRYDE
                     VLLTGRLTSALNRINPGMTDAVLHAALKEVERIHSPELLNNNETFHRLLTEGVKVSTQ
                     HEGNERGEIVWLIDFDTPDHNEFVVASQFTVIENNQNKRPDLVVFVNGIPLVVIELKN
                     AADENATLQSAFKQISTYKQAIPGLFTYNALLVISDGLEAKAGSLSAGFSRFMTWKIP
                     DDVDGMVGANNHLPQRVELETLIKGMLNKKTLLDLVRHFVVFEKTKKEDPQTGVISIS
                     TVKKIAAYHQYYAVNAAVASTLRAADMSTASKVMQNPTSYGLPDVTQQPKGDRKAGVV
                     WHTQGSGKSLSMVFYTGKIVLALNNPTIVVITDRNDLDDQLFDTFAACKQLLRQEPRQ
                     VENRQQLKALLRVASGGVIFTTIQKFQPEEGNVYEELSDRGNIVVIADEAHRTQYGFS
                     AKTVDDKNADGKVIGKKVVYGFAKYLRDALPNATYLGFTGTPIEKTDVNTPAVFGHYV
                     DIYDIAQAVEDGATVRIYYESRLVKVALSEEGRKLIKELDDELDQDELTDTQKAKAKW
                     TQMEALIGSERRIQNIAQDLISHFEARQEVFAGKGMMVCMSRRIAADLYREIIKLRPA
                     WHSDDLNKGVIKVVMTAASSDGPIMAQHHTTKQQRKLLAERMKDNNDELKLVIVRDMW
                     LTGFDAPSMHTLYIDKPMKGHNLMQAIARVNRVYGDKPGGLVVDYLGIASDLKEALSF
                     YSDAGGKGDPALMQEQAVQLMLEKLEVVSAMYHGFAYEDYFVADTAKKLVLILAAEDH
                     ILGLDDGKKRYINEVTALSLAFAIAIPHEQALAVKDEVAFFQAVKARLSKFDSTGSGK
                     TDETIETTIRQVIDRALVSEQVIDIFDAAGLKKPDISILSEDFLAELKDYQHQHVALE
                     VLKKLLNDELKVRAKTNLMQSRSLMEMLENAIKKYHNKILTAAEVIEELIKISKEIVA
                     SDKEAEKLHLSHYEYAFYTAVASNNSARELMQNDQLRELAIVLTQRVRQNASIDWTIK
                     ESVKAKLKVIVKRTLRQFGYPPDMQLLATEMVLKQAEMIANELAA"
BASE COUNT         2076 a         1654 c         1828 g         1806 t
ORIGIN      
        1 aaaacctccg gcgaaggcga catccgcaaa aacctcgttg cagaaggtaa tttgatcgat
       61 tgcatcgtca acctgcccgc caaattgttt ctgaacacgc agattcccgc ggcactgtgg
      121 tttttgaatc ggaatcgcgc gggaacaacc gtcggggtaa atgatgtacg ggcaaataac
      181 gttggggcaa ataatcattt gcccctacaa cacgatcatc cgcacaagaa tgaaattctg
      241 ttcatcgacg cccgtaacct cggccacctg atcaaccgcc gcacgcgcga attgtcgcac
      301 gacgacatcc acaaaattgc cagcacctac cacgcctggc gcaatgccgc cgtcgttccc
      361 gcgcaggcgg gaatccagcc gtatgaagac atcaaaggct tctgtgcctc agtgccgatt
      421 tcgcgcgtcg ccgaactgga ttacgtgctg acaccgggac gctacgtcgg cctgccagac
      481 gaagaagacg attttaattt tcccgaacgt tttgctgcgc tgagggcaga atttgaagcg
      541 caattgcggg aagaagcggt gctgaatcag gcgattgttg agagcttggc gagggtgaaa
      601 gtgtgagtaa gtggcaggaa tgtagattag gagaagttgc agaaattcag acaggacctt
      661 ttggtagtca actcaaaaat agccaatatg taactggcgg aacaccagta ataactgttg
      721 aacacataga taattttagg attatagact tcgactatcc cagtgttacc tatgaagata
      781 aaaaaagatt atcaaaatat ctactgaatc aaggtgacat tatttttact agagtcggtt
      841 ctgttgattt gagcgcttac gtccaacctc atcagcaaga ctggatgttt tcatccagaa
      901 tgcttcgtgt taggtcagat caaagaatta attcaagatt cttgagctac ttctttcaac
      961 aaaactcatt tagagaatac attctaagta ttgctgttgg agctacaatg ccatcaatca
     1021 atacagaaat tctgaaaagt gttcctattt catacccttc actcccagaa caaaaggcca
     1081 tcagctccgt cctcagcagc ttggacgaca aaatcgacct gctgcaccgc cagaacaaaa
     1141 ccctcgaagc catggctgaa acgctgttta ggcagtggtt tgtagaggaa gattcgggtt
     1201 tcagggaagg ctgtttagct gatgttgcaa ttaacgttaa agaagctata aaagcatcag
     1261 atttaaaatc ggaatcaaaa tatattggtt tggaacatat cgatcaaaga aatattgcac
     1321 tgaaacatta tggtttcggt tctgaagtca gcagtaataa gtatgcattt caaaggcacg
     1381 atatcttatt tggcaaatta cgcccatatt tccataaagt ttgtttcgca ccattttcag
     1441 gtatttgctc aacggatatt ttggtaatta ggccaataaa atcagaattg tttgcttttt
     1501 gtctttttgc atttttccag gatgaggtag ttgaatatgc caatttgagt tctgggggga
     1561 cgcgaatgcc gagaactgat tggacaacat taaagaatta tccaatagct atcccagatt
     1621 cttattctat tgaacgcttc aataatgctg tttcttcttc attgaaaaag atttcttcta
     1681 atttagagca aatccgcacc ctcgaaaccc tgcgcgacac cttgctcccc aaattgatga
     1741 gcggcgaggt gcgggtaacc gtatgatcga accgatagac aatatcgtga tttaccagcc
     1801 ggacgatggg ttgttggggg tggaagtgcg cttgcgcgac gaaacggctt ggttgtcgca
     1861 ggcacagatc gcggccttgt ttgataagga tagcgatacc atcgggttgc acctgaagaa
     1921 tatttaccaa tccggtgagt tgattgagtc ggcaactacc gaaaaatcct cggtagttcg
     1981 acaagaaggt gggcggcagg tcagaagaaa catcaaggtt tacaacctcg atgccatcat
     2041 ttccgtcggt tatcgcgtca actccaaaaa aggcacgcag ttccgtatct gggccagcaa
     2101 tattctcaaa cgttacctgg tgcaaggtta tgcgctgaat gaacagaaac ttcagatgca
     2161 acagcaaaaa ctcacggatt taaaacaagc gattgcttta tcttcacgtc tgttgcgaca
     2221 aaaagacctg actgtttccg aatcgcaagg tattttatcg atcctggaaa aatacagcca
     2281 cgcattgaca gtgctggacg attacgatca ccagcgctta caagtaacgg gaatccagca
     2341 aaccggacag agcagaattt cctatgatga agcaattcag caaatccggc tgtggcgtga
     2401 acaggagaaa ctgggtgggt tgttcggcaa cgaaaaagac gattccttca aaagctcgtt
     2461 ggaaaccatt taccaaacgt ttggcggcac agaactctat ccaagtgttg aagaaaaagc
     2521 ggctaatctg ttgtatttca tcgtcaagaa tcattctttt tccgatggca acaaacgcat
     2581 tgccgccgcg ctgtttgtct ggtttctggc gcgtcatgat tatctgctta acgcggatgg
     2641 agaaaagcgc atcgctgata atgccctggt tgcgtttacc ctgttgattg ccgagagcaa
     2701 acccgaggaa aaagacacga tggtgaaagt gaaagtcatc atcaatttaa tcaatggcaa
     2761 taatccgtag gggcaaataa tcatttgccc ctactatccc aacaataacg cggacaccat
     2821 gaccgacaac ccaaccagcc atcgccgcaa atcgattcga ttgcaggggt atgattattc
     2881 acaggctggt ttgtatttca ttactgtttg cacacacaac cgggtgccat tgtttgggga
     2941 aattgttgat ggggttatgg cgttgaatac agcgggtcag atcgtagaaa aatgctggtg
     3001 tacgataccc gagcattttc cgcaggttac agtggatgaa ttcgtggtga tgcccaatca
     3061 tgtgcatggg attattacca tcggtacaac cgttggggta aatcatgtgg gggcaaatga
     3121 cgttggggta aatgatgtag gggcaaatga ttatttgccc ctaccatcca acgaaacatc
     3181 cacgcaatcc aacgaaacac cccggtcatc gcaacatggt acatcccgca ccattggatc
     3241 gatggtgcgg ggattcaaaa ttggcgttac ccggtggttt cgcgccaata cggatattca
     3301 taaagtttgg cagcgcaatt attacgaaca tatcattcgc aatgaggata gttacctgaa
     3361 aatcgcagac tatattcaaa ctaatccgca gcgttgggaa atcgatactt attatgttta
     3421 agagtaaatg atgatttgcc cttaaacttg tcttcgtgca caataaagcg catcattcga
     3481 tgacatatct ggagataagg catgagaact accattgtat tggatgatga attactggaa
     3541 aaagcgcaag ccctgaccca tgttcaggaa aaatcagcct tggttaaaga agccttgaaa
     3601 gccctgatcg agcgggaaag tgccaaaagg ctggctaatt tgggtggctc tgaaccgcaa
     3661 ctggaagcca ttccccgccg acaaaccagc gaatagctgc cgcaatgatt ctggtcgata
     3721 cttcggtctg gattaaccat ctgcgcaata acgatccgta tttagtccgc ctgctgactg
     3781 agaacagcgt attaggtcat ccctttgttc gtagcgagct ggcgctgggc aacttgcgcc
     3841 aacgtgaaat cattctggct gcgctggata acctgcccca agcccctgtc gcttttaccg
     3901 atgaagtgaa ttatttcatc gaaaaacatg ctttatatgg tttgggtatc ggattgatcg
     3961 acgtgcatct gttagcttca acgctgttat ccggcaatac tcggctttgg acacaggata
     4021 agcggcttca ggcagctgca ttacgtctta atttggcggc actggttcac catgtctaaa
     4081 ctcaccgaat cagcgataga aaccttagcg atccaactgt tcgagcaact tggttacagc
     4141 gctatccacg cgcccgatat ttcccctgat ggggatcatc cggaacgcac ccgttatgat
     4201 gaggtgctgc ttaccgggcg cttaacaagt gcgcttaatc gcatcaatcc tggcatgacg
     4261 gacgcggtat tacacgctgc gttgaaggaa gttgagcgta ttcattcacc tgaattactc
     4321 aacaacaatg aaacctttca tcgcctgctg accgaaggcg tcaaagttag cacacaacac
     4381 gaaggcaacg aacgtggcga gatcgtctgg ctaattgatt ttgatacccc cgaccataac
     4441 gaatttgtcg tggctagcca gttcacggtg atagaaaata accagaacaa gcgcccggat
     4501 ctggtggtat ttgtgaatgg tatcccgctg gtggtgatcg agcttaaaaa tgccgccgat
     4561 gaaaacgcca cgctacaatc cgctttcaag caaattagca cctacaaaca agccatccct
     4621 ggtttgttta cttacaatgc cttgttggtg atttcggatg gattggaagc caaagccggt
     4681 tcgttatcgg cgggattcag ccgtttcatg acgtggaaga ttcctgatga tgttgacggt
     4741 atggtagggg caaataatca tttgccccaa cgggttgaat tggaaacgtt gatcaagggc
     4801 atgctcaaca aaaaaacctt gttggattta gtgcggcatt ttgtagtgtt tgaaaaaacc
     4861 aagaaagaag atccgcagac cggtgtgatc agtatcagca cagtcaagaa gattgccgcc
     4921 tatcaccaat actatgccgt caatgccgcc gtggcttcta cgttgcgtgc cgctgacatg
     4981 agcacagctt ccaaagtcat gcaaaacccg accagttatg ggctgccgga tgtgacgcag
     5041 caacccaagg gcgatagaaa agccggtgtc gtatggcaca cgcaaggcag cggcaaatca
     5101 ttgtcgatgg tgttttatac cggcaagatc gtgctggcgc tgaataatcc cacgattgtc
     5161 gtcattaccg atcgtaacga tctggatgat cagctattcg atacttttgc cgcgtgcaaa
     5221 caattgctgc gacaagaacc cagacaggtg gaaaaccgcc agcaattgaa agcgctgttg
     5281 cgtgtcgcct ccggtggtgt gattttcacc acgatccaga aattccagcc agaggaaggc
     5341 aatgtttatg aagaactctc agatcgtggc aacatcgtgg tgatcgccga tgaagcacat
     5401 cgcacacaat acggattctc ggctaaaacg gtggatgaca aaaatgctga cggtaaagtg
     5461 atcggcaaga aagtggtgta cggttttgcc aagtatttac gcgatgcgtt gccgaatgcg
     5521 acttatctgg gttttaccgg tacgccgatt gaaaaaaccg atgtcaacac gcctgcggtg
     5581 ttcggccatt atgtggatat ttacgatatt gcgcaggcgg tggaagacgg cgccacggtg
     5641 cgcatttact atgaaagccg cttggtaaaa gtcgcgctaa gcgaggaagg ccggaaactg
     5701 atcaaagagc tggatgatga gttggatcag gacgaactga ctgataccca gaaggctaaa
     5761 gccaagtgga cgcaaatgga agcactgatt ggcagcgagc gtcgcatcca gaacatcgcg
     5821 caggatctga tcagccattt tgaagcacgc caggaagtgt ttgccgggaa aggtatgatg
     5881 gtgtgcatgt cgcgtcgcat tgcggctgat ctttatcgtg agatcatcaa gctgcggcca
     5941 gcctggcatt ccgatgactt gaacaaaggc gtgatcaaag tggtgatgac ggcggcttcc
     6001 tccgatggac cgatcatggc gcaacatcat accactaagc agcagcgcaa gctgctggcg
     6061 gagcgcatga aggataacaa tgatgaacta aaactggtga tcgtgcgcga catgtggttg
     6121 accggattcg atgcgcccag tatgcacacc ctgtatatcg ataaacccat gaaaggccac
     6181 aacctgatgc aagccatcgc acgggtgaat cgtgtgtatg gtgacaaacc tggcggattg
     6241 gtggtggatt atctcggcat tgcttcggac ttgaaagaag cgttgtcgtt ttattccgat
     6301 gcgggcggca aaggcgaccc agcgttgatg caagaacaag cggtgcagct catgttggag
     6361 aaactcgagg tcgtatcggc gatgtatcat ggttttgcgt atgaagatta ttttgtagct
     6421 gatactgcga aaaagctggt actgattctg gccgctgaag atcatattct cggactggat
     6481 gatggcaaga aacgctatat caatgaagtg accgcattat ctctggcgtt tgctatcgct
     6541 attccgcatg aacaagcgct ggcagtcaaa gacgaagtgg ctttctttca ggccgtcaaa
     6601 gcacgcttat ccaaattcga cagtactggc agcggcaaaa ccgatgaaac tattgaaact
     6661 accatccggc aagtcatcga ccgggcgttg gtgtcggaac aagtcatcga tattttcgat
     6721 gccgccggac tcaagaagcc ggatatttcg atactttccg aagactttct ggcggaactt
     6781 aaagattatc agcaccagca tgtggctctg gaagtattga agaaattgct caatgatgag
     6841 ctgaaagtgc gtgccaaaac caatctaatg caaagccggt ctttgatgga aatgctggaa
     6901 aacgccatca agaaatatca taacaagatt ttaacggcgg ctgaggtcat tgaagaattg
     6961 atcaaaatca gtaaggaaat cgtcgcctcc gacaaagaag ccgagaagtt gcatttgtcc
     7021 cattatgaat atgccttcta caccgcagtt gccagtaaca acagcgcacg cgagctgatg
     7081 caaaatgacc aattgcgtga attggcgatc gtactgacgc agcgagtgcg gcaaaacgct
     7141 tcgatcgatt ggaccatcaa ggagagcgtg aaagccaagt tgaaagtgat cgtaaaacgc
     7201 acgctacggc aattcggcta tccaccggat atgcaattac tggcaactga aatggtactg
     7261 aagcaggctg agatgattgc gaatgaattg gctgcttgaa tagcgtattt agtcggtcct
     7321 gcaaaacgct taggggccct ataaatgttc gaattcatgc gaat
//
LOCUS       DAOQLJ010000113         7272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_4218_length_7272_cov_5.744354, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000113 DAOQLJ010000000
VERSION     DAOQLJ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4218_length_7272_cov_5.744354"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PK522_08390"
     CDS             <1..311
                     /locus_tag="PK522_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC41789.1"
                     /translation="FGFEYVGAFSHFIVVRVNDTLCLDFDNETQFESHHYAFKVSERE
                     FDDIFARLLAEKIPYGSGPGSANDMAINHNYGGRGVYFCDPNRHLLEMLTADYVMPPQ
                     "
     gene            complement(407..1618)
                     /locus_tag="PK522_08395"
     CDS             complement(407..1618)
                     /locus_tag="PK522_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HNC41790.1"
                     /translation="MPLVDMRDMLDHAYQNNYAIGGFGLVSLDFLHAILSAAEYCRSP
                     VIFNLSEPLFKHFGTQLILSSIEHAAHHAKVPVAIHFDHGESYESIVQAIKLGCNSVM
                     LDASKDTFPANATQTTRVTEMAHGCGVAVEGALGFVGGSEGENAISHLGEIIFTSAEE
                     AEAYVTRTHVDFLAVSIGTSHGRQPSRARLDFKRLKRIDETLKIPLVLHGGSGLVEEQ
                     YHKLILSGIAKINCYTELSDLAAHQIQANLNAQSYNGYIEAMTGVHDRLFERIKQYMQ
                     FWGSAGRAAEVLIQCRHWNSVEQIILFNPNQPQNIDLLSAQGSALLNKIPSVRNVFLG
                     KAITEPTRYQLCWHIQLSHEDALPHYLAHPSYTAFINQCCYTLSSERINITFTPSHFA
                     KNHELQHIDRL"
     gene            complement(1663..2019)
                     /locus_tag="PK522_08400"
     CDS             complement(1663..2019)
                     /locus_tag="PK522_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41791.1"
                     /translation="MIEADTLALPLRKFPKKINAKCYNRGRLAILRIGQPLRIALEHH
                     RGLEVILNNTIWLCVDSFADDQPVLAWREFRTQDRNQLHRPIACELWLYHSCAGLIMG
                     SALEDLQQTLEKLTLH"
     gene            complement(2063..2971)
                     /gene="cbbX"
                     /locus_tag="PK522_08405"
     CDS             complement(2063..2971)
                     /gene="cbbX"
                     /locus_tag="PK522_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbX protein"
                     /protein_id="HNC41792.1"
                     /translation="MTTETVSVDLEAEFQDSNIKEVLEKLDRELIGLKPVKTRIHEIA
                     ALLLVDRVRKQLGLSAGAPSLHMCFTGNPGTGKTTVALRMSEILHRLNYVREGHLVSV
                     TRDDLVGQYIGHTAPKTKEVIKKAMGGVLFIDEAYYLYKPENERDYGAESIEILLQTM
                     ENNRDDLVVILAGYKDRMDKFFYSNPGMRSRIAHHIDFPDYSANELLAIAKLMLAAQN
                     YRFSPSGENAFSEYLQLRMKLEHFANARSVRNALDRARLRQANRLFTSAKKTLTSIDL
                     MTIEEEDIRSSRVFLEGKLDTGSSKI"
     gene            complement(3104..3538)
                     /locus_tag="PK522_08410"
     CDS             complement(3104..3538)
                     /locus_tag="PK522_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HNC41793.1"
                     /translation="MMTNTGNRLTQGQFSFLPPLTDKQISSQIKYALKKGWAIGIEYT
                     DDPHPRNTYWEMFGNPMFDLKDPAGILMEINNCRKTFPNHYIRVTAFDSSRGVESPTM
                     SYIVNRPENEPGFSLMRQESSSRNINYTIHSYATDKPEGERY"
     gene            complement(3556..5031)
                     /locus_tag="PK522_08415"
     CDS             complement(3556..5031)
                     /locus_tag="PK522_08415"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase large subunit"
                     /protein_id="HNC41794.1"
                     /translation="MASETIKEGKERYKSGVIPYKKMGYWEPDYIPQDTDVIALFRIT
                     PQEGVEPEEAAAAVAGESSTATWTVVWTDRLTACELYRAKAYRVDPVPNTGPGTKTDQ
                     QYFAYIAYDLDLFEEGSIANLTASIIGNVFGFKAVKALRLEDMRIPVAYLKTFQGPAT
                     GIVVERERLDKFGRPLLGATTKPKLGLSGRNYGRVVYEGLKGGLDFMKDDENINSQPF
                     MHWRDRFLFCMEAVNKAAAATGEVKGHYLNVTAATMEDMYERAEFAKSLGSVIVMIDL
                     VVGYTAIQSMAKWARKNDMILHLHRAGNSTYSRQKNHGMNFRVICKWMRMAGVDHIHA
                     GTVVGKLEGDPLMIRGFYDTLRETNIERNLEHGIFFDQGWASLRKVMPVASGGIHAGQ
                     MHQLIDYLGEDVVLQFGGGTIGHPQGIQAGATANRVALEAMIMARNEGRDYVAEGPKI
                     LAEAAKWCSPLKLALDTWKDITFNYESTDTADFVPSTTMSQ"
     gene            5202..6158
                     /locus_tag="PK522_08420"
     CDS             5202..6158
                     /locus_tag="PK522_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNC41795.1"
                     /translation="MRHSTIRQLEVFEAIARLKSFTRAAEELFLTQPTVSMQIKKLTD
                     DIGLPLFEQVGKKIYLTDAGKELYQTCLGIFEHFERFEMIASNMRGLKAGKLRLAVVT
                     TAKYFTPRLLGMFCKKYPGIEVKLKVTNRERVLERLLHNQDDLYILGQVPEEIDAVAA
                     PFLDNPLVVLASTNHPLAKARNIPVQSLSEEAFIMREPGSGTRMATERFFTDRGLKIN
                     IRMELGSNEAIKQAIVGELGIAVLSRHTLALDAPMGQLVMLHVEGFPLKRSWYYAYPS
                     GKQLSIVAETFLEYLRQAPDFLVDLSCYHASVGCCPLLNKSN"
     gene            6366..6845
                     /gene="rnhA"
                     /locus_tag="PK522_08425"
     CDS             6366..6845
                     /gene="rnhA"
                     /locus_tag="PK522_08425"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNC41796.1"
                     /translation="MQNTVQKIEIYTDGACKGNPGVGGWGALVMYDGHTQELFGGEVS
                     TTNNQMELMAAIRALQALESLPLAESARVHLFTDSQYVQKGISEWIENWKKRGWRTAD
                     RKPVKNESLWKTLDQLSQRYSIEWCWVRGHTGHNGNERADALANRGVETVMVGNHSN"
     gene            6860..>7272
                     /gene="dnaQ"
                     /locus_tag="PK522_08430"
     CDS             6860..>7272
                     /gene="dnaQ"
                     /locus_tag="PK522_08430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HNC41797.1"
                     /translation="MRQIFLDTETTGLDVKSGHRIVEIAAVEVCNRQLTGRHFHYYLN
                     PERESEASALQIHGLTTEFLQDKPKFPDICEAFIQFIENAELLIHNAPFDVSFLNNEL
                     VLCNQNTLSSYCTEIIDTLILAKELHPGKRNNLDA"
BASE COUNT         1953 a         1484 c         1669 g         2166 t
ORIGIN      
        1 tgttcggttt tgagtacgtc ggcgcgtttt cgcattttat cgtggtgcgc gtcaacgata
       61 cgttgtgcct ggattttgac aatgaaacgc aatttgagtc acatcactac gcgttcaaag
      121 tttcggagcg agaattcgac gatattttcg cgcgattgct ggcggaaaaa attccgtacg
      181 gcagcggccc cggcagtgcc aacgatatgg cgatcaatca caactatggc ggccgtggcg
      241 tgtatttctg cgacccaaac cggcacttac tggaaatgtt gacggcggac tacgtgatgc
      301 caccgcaata acgggcaaga ttttgatagt taaagaaact gctaacgtta ccttatcgat
      361 tttggagaga tcaactgatg ttaagtcaaa gtagtacttt agggcgctac aacctgtcga
      421 tgtgttgtag ttcatggttc tttgcgaagt gactgggtgt aaacgtgata tttattcttt
      481 cagaagatag cgtgtagcag cattggttga taaacgcagt ataggatgga tgagcaagat
      541 aatggggtaa ggcatcttcg tgggaaagtt gaatgtgcca acacagttga tagcgcgtcg
      601 gttctgtgat ggctttacct aaaaacacat tgcgtacact ggggattttg tttaacaatg
      661 cgctaccttg agcggaaagc aaatcaatat tctgcggttg gttgggattg aataagataa
      721 tttgttctac tgaattccag tgccgacatt gaatgagtac ttcagcagct ctgcctgccg
      781 atccccagaa ttgcatgtat tgtttgattc gctcaaataa tcgatcatgc acacctgtca
      841 ttgcttcaat gtatccatta taagattgtg cattcagatt ggcttgaatc tgatgggctg
      901 ccaaatccga taactcggta tagcagttga ttttagcaat tccactcaag attagcttat
      961 ggtattgctc ttcgactaat ccactgccgc catgcagaac caggggaatt ttgagcgttt
     1021 catcgatgcg ttttagtcgc ttgaaatcca gcctggctcg actaggctgc cgtccatgag
     1081 aggtgccgat ggaaaccgcc aagaaatcaa cgtgagttct ggtgacataa gcttcagctt
     1141 cttcagccga agtgaaaatg atttcaccta aatgggagat cgcattttca ccttcacttc
     1201 cgccgacaaa acccaaagcg ccttctacgg caacgccaca gccatgtgcc atttcagtga
     1261 cacgagtcgt ttgtgtggca ttggccggaa atgtgtcttt tgaagcgtcc agcatgacgc
     1321 tgttacagcc taatttaatc gcttgtacga tcgattcata gctttcgccg tgatcgaaat
     1381 gaatggcaac cggtactttg gcatgatgtg ctgcgtgctc gatggatgac aatatgagtt
     1441 gagtaccgaa gtgcttgaat aacggttcgg agaggttaaa aataactggt gagcgacaat
     1501 attcagcagc actcaggatc gcatgtagaa aatctaagct aactaatcca aaaccaccga
     1561 ttgcgtagtt attttggtaa gcatggtcta gcatatctcg catatcgaca agtggcattt
     1621 cctttccccg tattcagagt cagcctgaat cgctaattgt gcctaatgaa gtgtgagttt
     1681 ttcgagggtt tgctgcaaat cttctaaagc cgaccccata atcaatcccg cacagctatg
     1741 atataaccat agctcgcatg cgattggtcg atgtaattga tttcgatctt gtgttctgaa
     1801 ttctcgccaa gcgagtacgg gttgatcatc tgcaaagcta tccacacata accaaatggt
     1861 gttatttaaa atcacttcta gtccacggtg gtgctcaagc gctattcgta gcggttggcc
     1921 tattcttaag atagcaagac gtccccggtt gtagcacttt gcatttattt tcttagggaa
     1981 tttacgtaat ggaagtgcaa gcgtatcggc ttctatcatc gcaatcctca tttttgttag
     2041 ttatctaatt ctgattagac ttttaaatct tgctgctacc agtatcgagc tttccttcta
     2101 aaaatacacg actggaccgg atatcttctt cttcaatggt cattaaatca atactggtta
     2161 aggttttttt cgcactggta aacagtcgat tggcttgcct caacctggcg cgatctaatg
     2221 cattgcgaac agaacgtgca ttggcaaagt gttcgagttt catccgtagc tggagatact
     2281 cgctaaaagc gttttctcct gatgggctga agcggtaatt ctgggcggcc agcattaatt
     2341 tagcaattgc taacagctca ttggcactgt aatcggggaa atcaatgtga tgggcgatgc
     2401 gcgaacgcat accggggtta ctataaaaga atttatccat ccgatcctta tagccagcaa
     2461 ggataacgac taaatcatcg cgattgtttt ccatcgtttg caataagatt tcgatcgatt
     2521 ctgcaccata gtcacgctcg ttctctggtt tatacagata ataggcttca tcgataaata
     2581 acacgccccc cattgccttc ttgataactt ctttggtttt aggggctgta tgaccgatat
     2641 attgtccaac taagtcatcg cgcgttacgg atactaagtg accttcccgg acataattca
     2701 gtcgatgcaa aatttcagac atccgcaatg cgacggttgt ttttccagtt cctgggttgc
     2761 cagtaaaaca catatgtagg cttggcgcac cagcggataa gcccagttgt ttgcgaacac
     2821 gatcaaccag tagaagtgcg gctatctcat gaattcgagt tttaactggt ttcaggccga
     2881 tcaattcccg atctagtttt tctagaactt ctttgatgtt cgagtcttga aattcagctt
     2941 ctaaatcgac tgaaaccgtt tcggttgtca ttagattttt tgttgtctta gccacgatgc
     3001 actccttgtt ctcgcattgc tagaatcggt tttaacttat attggtgagt aaaagcctaa
     3061 attttctctc tctttggata aagagagaga aagcaggttt aggttagtat cgttctcctt
     3121 caggtttatc tgtcgcataa gaatgaatgg tgtagttgat atttctgctg gagctttctt
     3181 gtcgcatcaa gctaaaacca ggttcatttt ctgggcggtt cacaatataa gacatagtag
     3241 gactttctac accgcgacta gagtcaaatg ctgttactct aatgtagtga ttcggaaacg
     3301 ttttgcgaca gttattgatt tccatcaaaa tccctgcagg atcttttaaa tcaaacattg
     3361 gattgccaaa catctcccag taggtattgc gtggatgagg gtcatccgta tattcgatgc
     3421 cgattgccca tccttttttg agtgcgtatt tgatttgtga tgagatctgc ttgtcagtaa
     3481 gcggcggcag gaaagagaat tgcccctgtg ttaggcgatt gccagtattt gtcatcatga
     3541 ttgcttctcc ttgggctatt gactcatggt tgtagaagga acgaaatcgg cggtgtcggt
     3601 agattcataa ttgaaggtga tatctttcca agtatctaat gcaagcttaa gcggtgagca
     3661 ccattttgct gcttctgcta gaatctttgg tccttccgca acataatcac gaccttcatt
     3721 acgcgccatg atcatcgctt ccagcgcaac gcgatttgcg gttgctcctg cttgaatacc
     3781 ttgtggatgg ccgatggttc ctccaccaaa ttgcagcact acgtcttcac ccaggtaatc
     3841 aatgagttga tgcatttgac ctgcatgaat tccacctgac gcgacgggca taacttttct
     3901 gagggaagcc catccctgat cgaagaagat tccgtgctca aggttacgct caatattggt
     3961 ttcgcggagt gtgtcataga atccccggat cattaatgga tcaccttcta gcttgcctac
     4021 aacagttcca gcatgaatat gatctacacc agccatacgc atccatttac aaatgactct
     4081 aaaattcatg ccatgatttt tctggcgcga ataagttgag ttacccgctc gatgtaagtg
     4141 caaaatcatg tcatttttac gcgcccattt cgccatggat tgaatcgcgg tatagccgac
     4201 gaccaaatca atcataacga tgactgatcc gagcgatttg gcgaattctg ctcgttcgta
     4261 catatcttcc atggtggctg cagttacatt gagataatgc ccttttacct ctcccgtggc
     4321 agcagcagct ttgttaaccg cttccataca gaataagaag cgatcacgcc agtgcatgaa
     4381 gggttgtgaa ttaatgtttt catcatcctt catgaaatca agaccacctt ttaggccttc
     4441 ataaacgaca cgtccataat tgcgaccaga aagtcctaat ttaggtttgg tggtagcgcc
     4501 taataggggg cgaccaaatt tatccagccg ttctcgttca acgacaatac cggttgcggg
     4561 accttggaat gttttaaggt acgctacggg gatacgcata tcttctaacc gcaatgcttt
     4621 gactgccttg aagccaaata cattaccgat aattgatgca gtcaaattgg caatggaacc
     4681 ttcttcaaat agatctaagt cataggcaat gtacgcaaaa tattgctgat ccgtttttgt
     4741 tcctggtcct gtattgggga caggatcaac acgataggct tttgctcgat agagttcgca
     4801 agctgtgagt cgatcagtcc agacaaccgt ccaggttgct gttgacgatt caccggcaac
     4861 tgccgcagca gcttcttctg gttctactcc ttcttgtggg gttattcgaa atagtgcaat
     4921 gacgtcagta tcttggggaa tgtaatcggg ttcccaatag cccatttttt tgtacgggat
     4981 cacgcctgat ttataccgtt ctttaccttc tttaatcgtt tcagaagcca tatttttctc
     5041 caaggtgtta aaaggatgtg gccacacgat gagtcatagg aattagcgca ttgtgggttg
     5101 gaattatggt agatttttat taccataaat aaaaatcaat tatttctata gtaatgatag
     5161 atataaatct atggtgacag ttttttaaga gggataaaac gatgcgtcac agcacaatac
     5221 gccagcttga ggtgtttgaa gccattgcac gtttgaaaag ctttactcgt gcagctgaag
     5281 aattatttct gacccagcca acggtatcga tgcaaatcaa gaaattaacc gatgatattg
     5341 gtttgccttt atttgagcag gttggaaaga aaatttattt aacggatgca gggaaagaac
     5401 tttatcaaac ctgtttaggc atatttgaac attttgagcg gtttgaaatg atcgcatcca
     5461 atatgagggg cctaaaagct ggaaagttga ggcttgcggt tgtgacaaca gcaaaatatt
     5521 ttacgccgcg cttattaggt atgttttgta aaaaatatcc cggaattgag gttaagctaa
     5581 aagtaacgaa tcgtgaacgg gttttagagc gattactcca taatcaggat gatctttata
     5641 ttttgggtca agttccggag gaaatcgatg ctgttgctgc tccttttcta gataatccat
     5701 tggttgttct ggcttcaaca aaccatcctc ttgccaaagc tagaaatatt ccagttcaat
     5761 cactgagtga agaagccttt atcatgcgag aaccaggttc tggtacccgt atggcaactg
     5821 aacgtttttt taccgatcgt gggttaaaaa tcaatatcag aatggaattg ggaagtaacg
     5881 aggcgatcaa acaagccatt gttggagagt tgggtattgc tgtactttcg cgacatacct
     5941 tggcattaga tgcgccgatg gggcagcttg ttatgttgca tgtagaaggt tttccactta
     6001 agcgctcctg gtattatgct tatccatccg gtaaacaatt atcgattgtg gcggagactt
     6061 ttcttgagta tttacgccag gcacctgatt ttctggtcga tttgtcgtgc taccatgctt
     6121 cagttggatg ctgtccgtta ttgaataaaa gtaattaaaa tcagatcgct taaatcattc
     6181 taagtactaa tcatgaactt aagtgatggt tgatagtgaa ctactcgtga aaacaagcca
     6241 tatatgacct gattgactgg taattgctgc aggccaggat tagaatgact aagatagatt
     6301 gactcgatga attgagagcg cacgtcagtt tataagggac acacgaatag tggatataac
     6361 ttgtgatgca aaatacagta caaaaaatcg aaatttatac agatggagct tgtaaaggta
     6421 accccggggt tggcggttgg ggtgccttgg ttatgtacga tgggcatact caagagttgt
     6481 ttggtggcga agtgagcacg acaaataatc aaatggagtt gatggcggcg attcgcgctt
     6541 tacaagcgtt agaatcatta ccgctagctg agtcggcgcg ggttcatctg tttactgatt
     6601 cacaatatgt acaaaagggg attagtgaat ggatcgagaa ttggaagaaa cgaggatggc
     6661 ggactgctga ccgcaagcca gttaaaaatg aatcattgtg gaaaacactt gatcaattat
     6721 cgcagcgcta ttcaattgaa tggtgctggg taagaggtca taccggtcat aatggcaatg
     6781 agcgagcaga cgcattggct aatcggggtg tggaaaccgt gatggttgga aatcattcaa
     6841 actgaagtat aaaccagtaa tgcgccaaat ttttcttgat actgaaacaa cgggtctcga
     6901 tgtaaagtcg ggtcaccgta tcgtggaaat cgctgctgtc gaagtttgta accgacaact
     6961 gacgggtcgc catttccatt attatttgaa tcctgagaga gaaagtgaag ccagtgcttt
     7021 acagatccat ggattgacta ccgaattctt acaagataag cctaaatttc ctgatatttg
     7081 cgaagcgttt attcagttta ttgaaaatgc agagttattg attcacaacg caccttttga
     7141 cgtgagtttc ttgaacaatg agttggtgtt gtgtaatcaa aatacactga gtagctattg
     7201 cactgagatc atcgatactt tgatcttggc gaaagagctt catccgggta aacgcaataa
     7261 tctcgatgct tt
//
LOCUS       DAOQLJ010000114         7238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_4250_length_7238_cov_5.391480, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000114 DAOQLJ010000000
VERSION     DAOQLJ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4250_length_7238_cov_5.391480"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PK522_08435"
     CDS             complement(<1..460)
                     /locus_tag="PK522_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNC41798.1"
                     /translation="MINIEINGKQLEVPKGSTVMDAAKQLGIYIPHFCYHKKLSIAAN
                     CRMCLVQIEKAPKPLPACATPATEGMKIFTHSDQAVLAQKGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGESASRYQEAKRVVTNKNLGPLISTDMTRCIHCTRCV"
     gene            complement(515..1792)
                     /gene="nuoF"
                     /locus_tag="PK522_08440"
     CDS             complement(515..1792)
                     /gene="nuoF"
                     /locus_tag="PK522_08440"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNC41799.1"
                     /translation="MTQHTQVILAGIDFADPDTWRLTNYLKRGGYKALRKIFSEKISP
                     EVVIDEVKKSALRGRGGAGFPTGLKWSFMPRQYEGEKYLVCNSDEGEPGTFKDRDIMR
                     FNPHILIEGMLIAAYAMGIRTGYNYVHGEIWDVYERMEEAVEEARSAGFLGNNILGSN
                     FSFQLYNHHGYGAYICGEETALLESIEGKKGQPRFKPPFPANFGLYGKPTTINNTETF
                     ASVPWIILNGGEKYLELGKPNNGGTKIFSVSGHVNKPGNYEVPMGTPFAELLELAGGM
                     RGGRKLKGCIPGGSSMPILPGEVMMNTNMDYDSIAKAGSMLGSGAVIIMDETTCMVKA
                     LERLAYFYYEESCGQCTPCREGTGWLYRIINRIEHGKGRQEDLDLLDNLADNIQGRTI
                     CALGDAAAMPVRAMLKHFRDEFAYHIEYKKCMV"
     gene            complement(1789..2265)
                     /gene="nuoE"
                     /locus_tag="PK522_08445"
     CDS             complement(1789..2265)
                     /gene="nuoE"
                     /locus_tag="PK522_08445"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNC41800.1"
                     /translation="MLSTESLKKIDKEIAKYPADQKQSAVMSALAIAQTEKGWLSSET
                     MDFVANYLDMPPIAVYEVATFYNMYNLKPIGQYKITVCTNLPCALSGGNDAANYLKQK
                     LGIGFNETSADGRFTLKEGECMGACGDAPVLLVNNKRMCSFMSNEQIDRLLEELKK"
     gene            complement(2268..3524)
                     /locus_tag="PK522_08450"
     CDS             complement(2268..3524)
                     /locus_tag="PK522_08450"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNC41801.1"
                     /translation="MAEIRNYTMNFGPQHPAAHGVLRLVMELDGEVIIRADPHIGLLH
                     RATEKLAESKTYIQSVPYMDRLDYVSMMVNEHAYVMAIERLLKIEVPLRAQYIRVMFD
                     EITRILNHLLWLGAHALDVGAMTVFLYAFRDREDLMDCYEAVSGARMHAAYYRPGGVY
                     RDLPDTMPQYQATDGVSDIQVIKQRNENRQGSLLDFIEDFTNRFPGYVDEYETLLTDN
                     RIWKQRLVDIGIVSPERAKALGFTGPMLRGSGIEWDLRKKQPYEVYDQVEFDIPVGVN
                     GDCYDRYLVRIEEMRQSNRIMKQCVAWLRENSGSVIVDNYKIAPPPRAAMKQNMEELI
                     HHFKLFTEGIHVPPGEIYAAVEHPKGEFGIYIISDGANKPYRLKIRAPGFAHLAALDE
                     MSRGHMIADLVAIIGTQDIVFGEIDR"
     gene            complement(3582..4193)
                     /locus_tag="PK522_08455"
     CDS             complement(3582..4193)
                     /locus_tag="PK522_08455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNC41802.1"
                     /translation="MSMSRAEKLASSIANILSNKLVDQAIHLNELTVVLESQDLLDVA
                     KILRDHPDLAFDTLIDLCGVDYSTYTNADFAGKARGGRRFAVVYHLLSIKHNHRLRVR
                     SFLNDDELPMIDSVIDIWPSANWFEREAFDLYGIVFVNHPDLRRILTDYGFIGNPFRK
                     DFPLSGHVEMRYDPDQKRVIYQPITIEPREITPYIIREEHYGD"
     gene            complement(4205..4675)
                     /locus_tag="PK522_08460"
     CDS             complement(4205..4675)
                     /locus_tag="PK522_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNC41803.1"
                     /translation="MDSTVDRGFITTSLDSVINWGRTGSMWPMTFGLACCAVEMMQTG
                     ASRYDLDRFGIVFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSCAN
                     GGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGILQLQNKIKRTNTIAR"
     gene            complement(4680..5036)
                     /locus_tag="PK522_08465"
     CDS             complement(4680..5036)
                     /locus_tag="PK522_08465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNC41804.1"
                     /translation="MLGNYFPILLFIVVGLLIGILSMLAGWFLAPNNPDDEKLSPYEC
                     GFSAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVVLEEIGFFGFVAMLVFLGIL
                     TVGFIYEWKKGALEWD"
     gene            5718..7232
                     /locus_tag="PK522_08470"
     CDS             5718..7232
                     /locus_tag="PK522_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC41805.1"
                     /translation="MGISTDPVTTKTTSRKTDFAYQAELRHNPKSFLKLILLGFSIVG
                     LPLIAALVYSAIRIDQLADQSRNTIYSATQITYGSSILIDEIIVMERSIQHAIILEDS
                     SLLESYFKAHDNFEKTATTLLEVSPYIEQQLLLEKLRLYETNIYREILNLSDTPQEFK
                     YLLDRFASLLILAQDFSTDNYQLIGSKVNEMSDLALHARTLVEWELLILIPFVILLAL
                     IFSVFIARPLRQIDEAIYDMGQGKLSRPVRVDGPQNLKYLGSRLDWMRRRLLKLEEQK
                     IQFLRHVSHELKTPLSAIREGVNLLAEGVAGDLNQKQNQIITILQTSSIQLQKRIEDL
                     LNFSALQSNKTALIKQRANLAYIITEVIKNQNLSILSRKIKIDLNCPKLILECDKQKI
                     STIIDNLLSNAVKFSPEASCIKIHAKQFDHIIWLDIIDSGVGVDIADEDKIFEPFYQG
                     RTAPISKIRGTGLGLSIAREYAYAHGGNIELIQHPDEPGAHFRLTLPVVDLEER"
BASE COUNT         2253 a         1609 c         1211 g         2165 t
ORIGIN      
        1 gaacacaccg tgtgcaatgt atgcagcgag tcatatcggt ggagataagt ggcccaagat
       61 tcttattcgt aacaacacgc tttgcctctt gatagcgaga agcgctttca ccataaccaa
      121 ccgcaagatc ttgcagctga cattcaccgc cttggtcaca aatcgggcaa tcgagtgggt
      181 gattaattag taagaactcc ataacaccct tctgcgctag aacagcctgg tcagaatgag
      241 tgaaaatttt catcccttct gttgcaggtg ttgcacatgc tggcaaaggt ttaggggctt
      301 tctctatttg caccaggcac attctgcaat tcgctgctat cgacaatttc ttatggtagc
      361 aaaaatgcgg gatataaatc ccaagctgtt ttgcagcgtc cattacagtg ctccctttgg
      421 gcacctccag ttgtttccca ttaatttcga tattaatcat cgatcagcta actcaaaaat
      481 tcttatgatt gaacggtaaa atttttatta cttatcacac catacatttt ttgtattcaa
      541 tgtgataagc aaattcatcg cgaaaatgct ttaacatagc ccgtacaggc attgctgcag
      601 catctcctaa tgcacaaatg gttcttcctt gtatgttatc agcgagatta tcaagtaaat
      661 ctaaatcttc ttgcctacct ttcccatgtt cgatccggtt aataatccga tacaaccatc
      721 ctgttccctc tctgcagggt gtgcactgac cacaggattc ttcataatag aaataggcta
      781 atctttccag tgctttaacc atacacgttg tctcatccat aatgatcaca gcgcctgagc
      841 caagcataga accagctttc gcaattgaat cataatccat atttgtattc atcatcacct
      901 ctcctggcaa gattggcatt gatgaaccac ctggaataca accctttaat tttcgacctc
      961 cccgcatccc tcctgctaat tctaataatt ctgcaaaggg agtccccatt ggcacttcat
     1021 aatttccagg tttattgaca tgtcctgata ctgaaaaaat tttagttccg ccattgtttg
     1081 gttttcctaa ttctaaatac ttctctcccc cattcagaat aatccaaggt acagatgcga
     1141 aagtttctgt gttattaatc gtcgtaggct ttccatataa gccaaaattt gctggaaaag
     1201 gcggtttaaa tcgtggttgc cccttttttc cttctattga ttccaataat gcagtttcct
     1261 caccgcatat gtatgcacca tagccatgat gattatagag ttggaagcta aaattcgacc
     1321 ctaaaatgtt attacctaaa aaaccagctg agcgcgcttc ttcgaccgct tcttccattc
     1381 gttcatagac atcccaaatt tctccatgaa catagttgta tcccgttcta attcccatcg
     1441 cataggcagc gatcaacatg ccttcaatta aaatatgtgg gttaaatcgc ataatatcgc
     1501 gatccttgaa agtacccggt tcaccctcat cagagttaca aaccaaatat ttctcgccct
     1561 catactgacg aggcataaaa ctccatttca atccggttgg aaagccagcg cctccacgac
     1621 cccgtaatgc agatttcttt acttcatcaa taacaacttc aggtgatatt ttttcactga
     1681 agattttcct taatgcctta tatccacctc gtttaaggta gttggttaat ctccaagtat
     1741 caggatctgc aaaatcaatt cctgctagga taacttgtgt atgctgagtc atttttttaa
     1801 ttcctctagc agtcgatcaa tttgctcatt tgacataaaa gagcacatac gtttgttatt
     1861 aactaacaat actggcgcgt caccacacgc tcccatgcat tcaccttctt ttaaggtaaa
     1921 tctgccgtct gcggaagttt cgttaaaacc aataccaagc ttctgcttta gatagtttgc
     1981 cgcatcattg ccgcccgata aggcacaagg taagttggtg caaaccgtaa tcttatattg
     2041 accaattggt ttcaagttgt acatattata aaatgtagca acctcataaa ccgctattgg
     2101 tggcatatct aagtaattcg caacaaaatc catggtttca ctcgaaagcc aacctttttc
     2161 agtttgtgca attgcaagcg cagacattac tgcagattgc ttctgatcag caggatattt
     2221 agcaatctct ttatcaattt tctttaggga ttcagtactt aacatgttta ccgatcaatt
     2281 tcaccaaaaa cgatatcttg cgtgccaata attgccacga ggtctgcaat catatgccct
     2341 cgagacattt catctaatgc tgccaaatgg gcaaaacccg gtgcacgaat ttttaaccga
     2401 taaggcttat ttgctccatc agagattata tatataccaa actctccttt aggatgctca
     2461 actgcagcat agatttcacc cggaggaaca tgaataccct ctgtaaaaag cttaaaatga
     2521 tggattaact cttccatatt ttgcttcatt gcagcacggg gaggaggcgc aattttataa
     2581 ttatctacaa ttactgaccc gctattttca cgaagccaag caacgcactg cttcatgatg
     2641 cgatttgatt gcctcatttc ttcaattcga acaagatacc gatcgtaaca atcaccattc
     2701 actcctaccg gtatatcaaa ttcgacttga tcgtagacct cataaggttg tttcttacgc
     2761 aaatcccatt caataccaga acctcgcaac atcggtcctg taaatcctag agctttagct
     2821 ctttcagggg aaacaatacc aatatcaact agccgttgct tccatattcg attatctgta
     2881 agcagcgtct catactcatc aacataacca ggaaatcgat tagtaaaatc ttcgataaaa
     2941 tccaacaaag aaccttgacg attttcgttt ctttgtttga ttacttgtat atcactcaca
     3001 ccatctgttg cttgatattg tggcatcgta tcaggcaaat cacgataaac accgcctgga
     3061 cgataataag cagcgtgcat ccttgcacca gatactgctt catagcaatc cattaaatct
     3121 tctctatcgc gaaatgcata cagaaagaca gtcattgcac caacatctaa tgcatgtgca
     3181 cctaaccaca aaagatggtt caatatccgc gtaatctcat cgaacatcac acgaatatac
     3241 tgggctcgta atggcacttc aattttgagt aaccgctcaa tcgccattac ataggcgtgc
     3301 tcattaacca tcatggacac gtaatccagg cgatccatgt agggaactga ttgaatatac
     3361 gttttacttt ctgccagttt ttctgttgcc cgatgtaata atccaatatg tggatcagcc
     3421 ctgattatta cttcaccatc aagctccatt actaatcgta atacaccgtg tgcagctggg
     3481 tgttgtgggc caaaattcat ggtgtaattt cgtatttcag ccatagctta ttttattttt
     3541 atagttaaaa aaagttgcat atcgatcaat ctaagctatg gctaatcacc ataatgttct
     3601 tcacgaataa tataaggtgt tatttcacgt ggctcgattg tgatgggttg atatataact
     3661 cgtttctgat ctggatcata tcgcatttca acatgaccag ataacgggaa atctttacgg
     3721 aagggattcc caataaatcc ataatcagta agaattctcc gcaaatcagg gtggttaaca
     3781 aaaactattc catataaatc gaaagcttcg cgctcgaacc aatttgctga aggccaaata
     3841 tcaatgaccg aatcaatcat gggtaattca tcatcgttaa gaaacgatct cacccttaaa
     3901 cgatggttat gtttaattga tagtaaatga taaacaaccg caaaccgccg acccccacgc
     3961 gcttttcctg caaaatcagc attggtataa gttgaatagt caacaccaca cagatcaatt
     4021 agcgtatcga atgctaagtc aggatggtcg cgcaaaattt ttgcgacatc caacaaatct
     4081 tgcgattcca gcactacggt taactcattt agatgaattg cctgatctac taatttatta
     4141 cttagaatat tagcgatcga cgacgcaagt ttttcagcgc gactcattga cataaaattg
     4201 gtatttaacg agcgatagta ttagttcttt ttatcttatt ctgcaattga agaataccat
     4261 aaagcagtgc ttctgctgtt ggtggacagc ctggaacata aatatcaacg ggcacgattc
     4321 gatcacaacc acgtacgact gagtaagaat aatggtaata tccgccccca ttggcacacg
     4381 atcccatgga tataacccag cgtggctcag ccatttgatc atatactttt cttaaagcgg
     4441 gcgccatttt attgcagagc gttcccgcta ctatcataac atccgattgt cgagggcttg
     4501 gtctaaaaac aattccaaat cgatcaagat cgtaacgcga agcccccgtt tgcatcatct
     4561 caaccgcaca acaagccaat ccaaaagtca ttggccacat cgaacctgta cgaccccaat
     4621 tgatgaccga atctaagctt gtcgttataa atccgcgatc tacagtacta tccataaatt
     4681 taatcccact ctaaagcgcc ctttttccat tcgtagataa aacccacggt aagaatgccc
     4741 agaaatacca acatagcaac gaatccaaaa aatccgattt cttctaatac aaccgcccaa
     4801 ggaaacaaga aagcaatctc tagatcaaat agaatgaata aaattgcgac caaataatac
     4861 cgaacatcaa acttcatcct ggcgtcttca aaagcggaga atccacactc ataaggcgat
     4921 aacttttcat catccggatt atttggtgct aaaaaccagc ctgccaacat tgataaaata
     4981 cctatcaata atccaacaac aataaaaagc aatattggga aataattacc aagcattgta
     5041 ttaatcataa aaattgattt tattcttctt atattattga cctaaggcta gtcaaataaa
     5101 ccactgcata agttatgagg gttattggaa ttgtatacct tgttattttt tagttaagtt
     5161 tatgttaagt atttttagta ttttataccg aataggatgg ttataacgcg atttatctaa
     5221 aaagatagct aactagcgtt caatataaca acactctcct cttctcatca gcacctttaa
     5281 cacaaaaact taagggcacg ttgagtagaa tgttaacata ggactattaa ctactcaaat
     5341 gaaggaatgc tagaattgat tagcctatga ctataaagct cccttcgtct aatcaattca
     5401 atatcgtatg agtcctcgta taacaaccac aaacagttga atttttttat ttatatcttt
     5461 acaagagatt attaaaagct aacacaccta accttattca ccatgcaatc aaaccggaag
     5521 aaccaaaata attcataatc aaagtagtta tcttctttta aaattttgtt atcgcatcat
     5581 aaagcggttg ttatatactc aaaaaaaata gagtgaccga ttgtagtacg gctatctaac
     5641 ttatcaatca ttaaagatta aaggtaaata tagatatgaa gctgaatttc ttattgaaat
     5701 aataggatat atcaacaatg ggaatttcta ctgatcccgt cacaactaaa actactagcc
     5761 gaaaaactga ttttgcctac caagcagaat taagacacaa cccaaaatcc tttctgaagt
     5821 taattttgct tgggttctca attgtaggct tacctttgat tgctgcttta gtttatagcg
     5881 cgattcggat tgatcaatta gcagaccaaa gccggaatac aatttatagc gctacgcaaa
     5941 ttacctatgg aagcagtatc cttatcgatg aaatcatcgt aatggagcgc agtattcagc
     6001 atgctattat tttagaagac tcgtctttgc tcgaaagtta ttttaaagct catgacaact
     6061 ttgaaaaaac agccaccacg ctattggaag tctcacctta catcgagcaa cagcttttac
     6121 tcgagaaact aagactgtat gagacaaata tttatcgtga aattcttaat ctatctgata
     6181 cgcctcaaga atttaaatat ttattggatc gctttgccag tttgcttatc cttgctcagg
     6241 acttctccac agataattat caattaattg gtagtaaagt taatgaaatg agtgacttag
     6301 ctctacatgc cagaactttg gtcgaatggg aactgcttat ccttattcca tttgtaattt
     6361 tacttgcact cattttttca gtttttattg cccgacctct tcgccaaatc gatgaagcaa
     6421 tctatgatat ggggcaagga aagctttcac gccctgtgcg agttgatgga cctcaaaatc
     6481 ttaagtattt aggttctcga ttggattgga tgcgccgccg acttctcaag cttgaagaac
     6541 agaaaattca gtttctacgc catgtttcgc atgagcttaa aacacctttg agtgcgattc
     6601 gcgaaggtgt caatcttctg gcggaaggag tggccggtga tcttaaccaa aaacaaaacc
     6661 aaatcattac tattcttcaa acgagcagta tccaattaca aaaacgtatt gaagatttgc
     6721 taaattttag cgcactccaa tccaataaaa ctgctttaat aaagcaacgt gcaaatctag
     6781 cctatatcat tacagaagta ataaaaaacc agaatctttc aattcttagt cggaaaatta
     6841 aaatcgattt gaactgccca aaattaattc tagaatgtga taagcagaaa atcagtacca
     6901 tcatcgataa tttactttcc aacgctgtaa aattctcgcc agaagcaagc tgcataaaaa
     6961 ttcatgctaa gcaattcgac catattatct ggcttgatat catcgattca ggtgttggtg
     7021 ttgatatcgc agatgaagat aaaatatttg aacccttcta tcaaggccgc acagccccca
     7081 tcagcaaaat tcgtggaaca ggtcttggat tatccatagc gagagaatac gcatacgctc
     7141 atggcgggaa tatcgaactt attcaacatc ctgatgaacc cggggcccat tttcgtttaa
     7201 cgctcccagt tgtagatctt gaggaacgat aattggta
//
LOCUS       DAOQLJ010000115         7121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_4362_length_7121_cov_6.393716, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000115 DAOQLJ010000000
VERSION     DAOQLJ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4362_length_7121_cov_6.393716"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1687
                     /locus_tag="PK522_08475"
     CDS             245..1687
                     /locus_tag="PK522_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41806.1"
                     /translation="MEPLVHFDKIIVEKPKVSKDCFYTNYILVRKGKSITCELRHKYE
                     ESIEGDNAQRYANLTSGIPAINHGLFAKEICFEFDLGPKEYNFFDEMLKKMSIEIFVS
                     RFYHDPLFIKPAFIAKQADVKLSNAMCQAQFTAGIGHDIANYPADPDKIVVLSSGGKE
                     SLLCYGLLNKAGMDVYPIYINESGGHWRTALTAYRSFKQTDTKTKRIWSNIDRLYAFM
                     VRNSPVVENNIRGIKSITDPLHFFTFAHYVFASLPLMEKSRIGHVCVGNEYVEPTKIL
                     TERHKINDVEFFPGIYDQSQAFDRYMTEWFESAGYGIKQWAPVRTITGYQVQKTLALD
                     YPGLFKLQTSCHHAHFKNGKILPCGTCQKCMRILAFCIANQLDYTLIGYTADNVAWLQ
                     SNIHRLVLAGDQELEHTFYQLGQQGFTVKGQRHDHIEQLHFDTHSSDVSNVPLQWRNR
                     IYPLLLGKATGMVQLTEDHQWQSIDIHEIS"
     gene            complement(1684..2568)
                     /locus_tag="PK522_08480"
     CDS             complement(1684..2568)
                     /locus_tag="PK522_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41807.1"
                     /translation="MNPFSVDHISCSNKQDHFNRYPMAKSTVTAYACLHRRIALLFLL
                     GLIYLSGCETVPLQDRNYADRGGFWEQGTSTQARFHHQAFDKDDEIGVMYASGRNTIL
                     NGSAINLSSDLDNHSVISNGPQSSARLEFKGDDEDDGDTCVINIDRFNRGDAYADTYD
                     CWYHIKTPHALLKAKNAILHIDVTQNETEVVVISGTIKVFTHGDPIQSIDVNSDQEVI
                     ISHQMLHRPRSISPDEIWNKISWRAQFQLYKTVIDWDQVVRGAIIVGIVAAIVAIGVA
                     TKGVGLSGLHRSRGFSRH"
     gene            2764..3312
                     /locus_tag="PK522_08485"
     CDS             2764..3312
                     /locus_tag="PK522_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNC41808.1"
                     /translation="MSRVTKKAINQYPLLARLIFWAQKLKYGVTLEPAWYWGRSPKLL
                     YGLQVFYRCLDRKQSPLNRRLRALVSNRIAQINTCAFCMDISTSVLQHQAVTFEMLGI
                     LADYESNSNFSESERAALAYAEAMTDSAQRVNDVLFQRLSQSFSEEQIVELTAWIAFQ
                     NLSSKFNAALDIPAQGFCQTPK"
     gene            3397..4116
                     /locus_tag="PK522_08490"
     CDS             3397..4116
                     /locus_tag="PK522_08490"
                     /inference="COORDINATES: protein motif:HMM:NF022633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HNC41809.1"
                     /translation="MIVYHFISPESALKALRDRRLKMTRLNELNDLHELCIANLFDSD
                     TKRELEVFKNRVNAQYGIICFSAIYHDPVLWSHYADSHRGVALVFDIPDHKAITINYQ
                     PEQYKQIINTAIHCGEFESFGLSKLIATKFSSWHYEQEVRMICDLNDHFCQIDAKGNK
                     AYFESLSLEPFGKDALKLVGLIRGVRCDLNPMDIAGELLAADTLSIQDASIDSSTFQI
                     TSSNAYPIHGVRSFSACCFRV"
     gene            4356..4784
                     /locus_tag="PK522_08495"
     CDS             4356..4784
                     /locus_tag="PK522_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41810.1"
                     /translation="MSPTEIQAKLQAMGYAQVSHTTFVKERPLAGGRQVIYRIEIEHT
                     PNSRKLGYFRSESGGRNKSPVTKTLPIPEQEAKWVNTLYELVCSDIPSEISVLRQCEP
                     ASEVSVRFGQGNPLSLSPTITTQLAASAGSINLNIIYHKQ"
     gene            4830..6041
                     /locus_tag="PK522_08500"
     CDS             4830..6041
                     /locus_tag="PK522_08500"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC41811.1"
                     /translation="MNYRFVIWIIVGLVLSCTGCVHRQYVTEPYPRWGTEIKVDQLGS
                     AEHLIVRIAYPPDIRDARACLLLVHGMNEYIGRYAGVADYFAKNFLVAGFDLYAHGLS
                     HPSLHQADLALNAGAHTHEIKDAYLAQLPLKNLDLLRKSLDQALQQLISICSERGLEG
                     KPIFIVSHSLGSLITASYLLQHQHMQAYAQQIAGVIFLGPAFAVTEVPGWRGWFANPF
                     IKLSFHAEEQHLNLHDELSPRVMANQALSLIVVSVLDGIFEVLSWPGLRSLFSPTTPS
                     WVVDYLTNDEQERARIRSDGWFIRQTLLRYVKGIEAEIVAFRSQMSEFKWPYYLIYSE
                     HDPITASWGNHDFAQTTLRNHSDNQILAYPELNHHEHLFSAQPLRNEILEKIVQWLDR
                     RLTHLQGSDDD"
     gene            6195..6794
                     /locus_tag="PK522_08505"
     CDS             6195..6794
                     /locus_tag="PK522_08505"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNC41812.1"
                     /translation="MSLVTRPAPDFTKPAILPNGSFEDNYNLHSHIRDKYAVLFFYPL
                     DFTFVCPSEIIAHSHRAKEFQQRNVEVIGISVDSQFTHHAWRNTPITQGGIGPIDLTL
                     VADLGGEIMTAYGVNLPNHIALRGSFLIDRKGIVRHEVVNDLPLGRDVDEMLRMVDAL
                     RFFEEHGELCPAGWKNGEPGMKASPEGVADYLSKHAEKL"
     gene            complement(6931..7005)
                     /locus_tag="PK522_08510"
     tRNA            complement(6931..7005)
                     /locus_tag="PK522_08510"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6971..6973),aa:His,seq:gtg)
BASE COUNT         2013 a         1407 c         1517 g         2184 t
ORIGIN      
        1 aatgcctgtt tgagtcacac ccagattaaa ctgaaaatag tttagaggca tataacgaat
       61 tgatcatcgt aggttttctg tagccaaata gtcaggttca acgtgacaat agatttctaa
      121 atacactcct ttattaacct gctaaaaaag ctaaattcaa ttttcgaagt tatctacaca
      181 atgacttaac aagtgattca gtagaatctt tatttctact tacgattagg tatttattcc
      241 tagtatggaa ccgcttgttc atttcgataa aatcattgtc gaaaaaccca aagtttcgaa
      301 ggattgtttt tacaccaact atatccttgt tcgcaaaggt aaatctatta cttgcgaact
      361 tcggcacaaa tatgaagaaa gtatcgaggg agataacgcg cagcgctacg ccaatttgac
      421 atcgggtatt ccagctatca accatgggct ttttgctaaa gaaatttgct tcgagtttga
      481 tctagggccc aaagaataca atttcttcga tgagatgctc aagaagatga gtatcgagat
      541 ttttgtaagc cgcttttatc acgatccact ttttatcaaa cctgccttta tcgccaagca
      601 agctgatgta aaactttcca atgctatgtg ccaagcccaa ttcacagcgg gtatcggcca
      661 tgatattgct aattatccag ctgatcccga taagatcgtg gtcctttctt ccggtggaaa
      721 agagagttta ctttgctatg gtttactcaa taaagcaggt atggatgttt atcccattta
      781 tatcaatgaa tctggtggtc attggcgaac agcgcttaca gcttatcgat cattcaaaca
      841 aacggatact aagactaaac gtatttggtc aaatattgat cggctgtatg cgtttatggt
      901 gagaaattca ccggtcgtcg agaataatat acgtggtatc aaatccatta ctgatccgct
      961 gcattttttt acgtttgcgc attatgtatt tgcttcgttg ccactcatgg aaaaatcaag
     1021 aattggtcat gtttgcgttg gtaatgaata tgtcgaacct actaaaattt taactgagcg
     1081 ccataagatc aatgatgtcg aattttttcc cggaatctac gatcagagtc aggcatttga
     1141 tcgttatatg accgaatggt ttgaatccgc agggtacggt atcaaacaat gggcgccagt
     1201 tcgcactatc actggctatc aagtgcaaaa aacgctagcg ctagattatc ctggattatt
     1261 taaattacag acttcctgcc atcatgccca tttcaaaaat ggcaaaatac tcccatgcgg
     1321 cacgtgtcag aagtgtatgc gaattttagc attttgtatc gccaatcaac tggattacac
     1381 cctgattgga tatactgcag acaacgtagc ttggttgcaa tccaatatcc atcgattggt
     1441 attagcgggt gatcaagagt tggagcatac cttctatcaa ttaggccaac aaggctttac
     1501 cgttaaaggc caacggcacg atcatattga gcaacttcac ttcgatacac attcatcgga
     1561 tgttagcaat gtaccgcttc agtggcgaaa taggatttac ccactattac tcggaaaagc
     1621 taccggcatg gtacaactta ctgaagatca tcaatggcaa tcgatagata tccacgaaat
     1681 aagttaatgt ctgctaaatc cccgagatcg atgaagtcca ctcaatccca cacctttggt
     1741 tgccacgcca attgcgacaa tggctgcaac aattccaacg ataatagcgc cacgcactac
     1801 ttgatcccaa tcgatgacgg ttttatacaa ttgaaattgc gcacgccaac tgattttatt
     1861 ccaaatttca tcgggtgaaa tggaacgtgg gcgatggagc atttgatgcg aaatgatgac
     1921 ttcttgatca ctgtttacat cgatagattg tatggggtct ccatgagtaa acactttaat
     1981 ggtgccgcta ataacgacta cttccgtttc attttgagtc acgtcaatat gtagtattgc
     2041 atttttagct tttaatagtg cgtgcggtgt tttgatgtga taccagcaat cgtaggtgtc
     2101 ggcgtaggca tctcccctat taaatcggtc gatattaatt acacacgtgt ccccatcatc
     2161 ttcatcgtca cccttaaatt cgagtcgggc tgaactttgc ggtccgttac tgatgactga
     2221 gtgattatca agatctgaag ataagttgat agccgatcca tttaatatgg tattgcggcc
     2281 ggatgcgtac attactccaa tctcatcatc tttatcaaaa gcctgatgat gaaagcgtgc
     2341 ttgggtagaa gtgccttgct cccagaaccc ccctctatcg gcataattgc gatcttgtaa
     2401 tgggactgtt tcgcaaccgc ttagatatat caaccccaat aaaaatagta gtgcaatgcg
     2461 gcgatgtaag catgcatatg ctgttactgt gcttttagcc attggatatc ggttaaaatg
     2521 atcttgcttg ttactgcaag agatatgatc tacgctgaac ggattcattt tgctgctcct
     2581 ctcagtctga ttcggtttag atgaatgatt gatggagatt actctacgct catctatctg
     2641 aatcgcgact gaataaggcg ttaaaaatac atattcaggg agattggaaa aagctaggat
     2701 aaaaccctaa agattaagtg atcgaatgga aatcacgcaa gaacagaaga ggagtctaat
     2761 tacatgtcgc gagtcactaa aaaagccatt aatcagtatc cattgttagc ccgcttaatt
     2821 ttttgggcac agaaactgaa atatggtgta acgcttgagc cagcatggta ttgggggcgt
     2881 tcaccgaaat tgctgtatgg tctacaagtt ttttatcgct gcttagatcg caagcaatct
     2941 ccgttgaatc gacgattacg ggcattggtt tcaaatcgga ttgcacaaat caatacttgt
     3001 gcattttgta tggatatcag tacctcagtc ttgcaacatc aagcggtaac atttgaaatg
     3061 ctgggtatat tggctgatta cgaatcaaat tctaactttt cagaatcaga gcgagctgct
     3121 ttggcctacg ctgaggcaat gacggattca gcccaacgtg tgaatgacgt tctttttcag
     3181 cgtttgagtc aatccttttc tgaagaacag atcgtagagc tcaccgcttg gattgctttc
     3241 cagaatcttt caagtaaatt taatgcggca cttgatatcc ctgcgcaagg gttttgtcaa
     3301 acaccgaagt gattcaataa atcgatatat tagtagtgtt aggctggatt ttttttgtaa
     3361 aagctattta aaagagaata tttcaagtaa tttttcatga ttgtttatca ttttatttcg
     3421 cctgagtcgg cgcttaaagc attacgtgat cgtcgtctga aaatgacacg tctcaatgag
     3481 ctaaatgatt tgcacgaatt gtgtattgct aatttgtttg attcggatac caaacgtgag
     3541 ttagaagttt tcaagaatcg agtgaatgcc caatatggaa ttatttgttt ctcggcgatt
     3601 taccatgatc ctgttctatg gagtcactat gctgatagcc atcgtggcgt tgctctagtg
     3661 tttgatattc ctgatcataa ggcaattaca atcaattatc agcctgagca atataaacaa
     3721 atcatcaata ctgcgattca ttgtggtgag tttgaatctt ttggtttgag taaattaata
     3781 gccacaaaat tttcaagctg gcattatgaa caagaggttc ggatgatctg tgatttgaac
     3841 gatcattttt gtcaaatcga tgcaaaagga aataaagctt attttgaatc gctcagtttg
     3901 gaaccattcg gtaaggacgc tttaaaatta gttggattga taaggggtgt tcgatgtgat
     3961 ttgaacccca tggatatagc aggtgagtta ctcgcggccg atacgctttc tatccaagat
     4021 gcgagtatag attcttcgac cttccagatt acatctagta acgcttatcc aatccatggc
     4081 gtgcggtcat tctccgcttg ttgctttagg gtctgaccgt gatgagatcg agagaattga
     4141 tagcgttgtc gaattgaaga tttgttacta atcaggaggt aaatttcatg ttttctcatt
     4201 gtttttttac gcatattacc tcccgaatca tactcttagt tgtattttta tttatacatt
     4261 catcaagctc ctttgcttta aataaccagt cgaatgttga ctcggatttg ctgacgagtt
     4321 ttcttaattt aactgttgaa ggggtttctc tcagtatgtc accgactgaa atccaagcta
     4381 agttacaagc aatgggttat gctcaagtga gtcacacgac ttttgttaaa gaaagaccac
     4441 tcgcaggggg acggcaagtg atttatcgca ttgaaattga acatacgcct aacagtcgta
     4501 aattgggtta ttttcgctca gaaagtggtg gaaggaacaa gagtcccgtg acgaaaacat
     4561 taccgattcc tgagcaggaa gcaaagtggg taaatacgct ttatgaatta gtgtgttcag
     4621 atatacccag tgaaattagc gtgttgcgcc agtgtgaacc ggcctcagag gtaagcgttc
     4681 gatttggtca gggtaatccg ctttcgttat ctccaaccat caccacacag ttagcggcga
     4741 gtgctggaag tatcaactta aacattattt atcataaaca gtaaccccag aaattagagc
     4801 tgttttagtt atgctgtgat taccaaccct tgaattatcg attcgttatt tggattatcg
     4861 tcggattagt actgagttgt acaggttgcg ttcatcggca atatgtgact gaaccctatc
     4921 cacggtgggg aacggaaatc aaagttgatc aactgggcag cgctgagcac ctgattgttc
     4981 gcatagccta tccaccggat attcgtgatg cgagggcctg cttattgtta gtccacggga
     5041 tgaacgaata catcggtcgt tatgctggtg ttgccgatta ttttgcaaaa aactttctgg
     5101 ttgcaggatt tgatttatac gcccatggtc tttctcatcc ttcattacac caagctgacc
     5161 ttgcattgaa tgcgggtgct cacacgcatg aaattaaaga tgcctattta gctcaacttc
     5221 cacttaagaa ccttgatctt ttaagaaaaa gtttagatca ggcgcttcaa caattgattt
     5281 cgatttgtag tgagcggggt cttgaaggga aaccgatttt tattgtttca catagtttgg
     5341 gatctttgat tacagcttcc taccttctcc agcatcaaca tatgcaggct tacgctcagc
     5401 aaattgcggg ggtaattttt ttagggcctg catttgctgt tactgaagta cccggctggc
     5461 gtggctggtt tgctaatcca tttattaagc tttcatttca tgcggaagaa cagcacctta
     5521 atttgcacga cgaactatca ccgcgtgtga tggctaatca agcactctca ctcattgtag
     5581 tatcggtgct agacgggata tttgaggtgt tatcctggcc tgggctgcga tcactgtttt
     5641 ctccaactac accgagctgg gtagttgatt atcttactaa cgatgagcag gaaagagcgc
     5701 gaattcgttc agatggatgg tttattcgcc aaactttact gcgttatgtg aaaggaatcg
     5761 aagcggaaat tgtagctttc cgcagccaga tgtctgaatt taagtggcct tattatttga
     5821 tttattcaga acatgatccg ataacggcaa gctggggtaa tcatgatttt gcccaaacaa
     5881 cattacgtaa ccactcggat aatcaaatct tggcgtatcc ggaactgaat caccatgagc
     5941 acctcttttc tgcacaaccc ttgcgtaacg aaattcttga aaaaattgtg caatggttag
     6001 atcgccggct gactcatttg caaggaagtg acgatgattg acactctaac gattttctct
     6061 attttcttgc aataaagttt gttgtgcagc aggattaatc ttaaaatatt cacttagcct
     6121 gactataaaa taaaaatagg caattatcgt tcgtcacata ttagattatt taataacaca
     6181 gaggaaaatt acgcatgtca cttgttacac gtcccgcacc agattttacg aaaccagcta
     6241 ttctaccgaa tggaagtttt gaagataatt acaatcttca ttcacatatt cgtgataaat
     6301 acgcggtcct ttttttctat cctctggatt ttacctttgt ttgcccatca gaaattattg
     6361 cacattcgca tcgcgccaaa gaatttcagc aacgcaatgt tgaggtaatc gggatatcag
     6421 tggactcaca attcacgcac catgcgtggc gtaatacacc gattactcag ggaggtattg
     6481 gccccattga tcttaccttg gttgcagatt tgggcggtga aatcatgact gcatatggcg
     6541 tcaatcttcc aaatcatatt gcgttaagag gatctttttt gattgatcgc aaaggtattg
     6601 tgcgtcacga agttgtaaat gatctgccac ttgggcgtga tgtggatgaa atgttgcgca
     6661 tggtggatgc acttcgtttc tttgaggaac atggggagtt gtgtccggct ggttggaaga
     6721 atggagagcc gggtatgaaa gcaagcccag aaggcgtagc tgactatctc agtaaacacg
     6781 cagaaaaatt ataatttttg atttttttat gattctcttt gcggatttag ttggttcgta
     6841 aagagaatca actaaatcga atggctattg ttggagtttc aaaagaaaat ttagagggcc
     6901 ttgccgtttg actgctcggt tagagataag tggggtggct gatgggggtc gaacccacga
     6961 caaccggaat cacaatccgg gactctacca ctgagctaca gccaccatcc taaatcgata
     7021 gagaacttac tataactagt ttctttttaa aaaagaaaaa aacctctggc gtgcccgaca
     7081 ggaatcgaac ctgtaacccc cagcttagaa ggctggtgct c
//
LOCUS       DAOQLJ010000116         7055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_4430_length_7055_cov_5.732714, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000116 DAOQLJ010000000
VERSION     DAOQLJ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4430_length_7055_cov_5.732714"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PK522_08515"
     CDS             <1..467
                     /locus_tag="PK522_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC41813.1"
                     /translation="LPMRELHCLDEALIRDLVMLKLKMVEVGAEHANKRPAELSGGMI
                     KRVALARALALNPELLFLDEPTAGLDPSLSESIVDLVRALREELTLTIVMVTHDLDTL
                     AAISTRIAVLADQHIVALGKLQEVVQVDHPFIKDFFWGERGQNALRKYLIAD"
     gene            563..1696
                     /gene="dapE"
                     /locus_tag="PK522_08520"
     CDS             563..1696
                     /gene="dapE"
                     /locus_tag="PK522_08520"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HNC41814.1"
                     /translation="MSGDTLTLAQMLISRRSLTPRDDGCQKLLENRLKALGFQIEKLN
                     FGDVENLWARRGTKQPLVCFAGHTDVVPTGPVGEWESDPFVPTIRDNRLYGRGAADMK
                     ASLAAFITAIESFLEANPEPEGSIALLITSDEEGPAVDGTVKVVEILKERGESIDFCI
                     VGEPTCTDQFGDTIKNGRRGSLSGHLTIKGIQGHIAYPHLAKNPIHLAAPVIAELAQT
                     VWDNGNEYFPPTTWHISNIQGGTGATNVIPGHISLIFNFRFATASTPESLQKKFCEIL
                     DKHGLEYQITWELSGKPYLTPRGKLADALTEAIKTVTTITPQLSATGGTSDGRFIAEI
                     CPQVVEFGPRNATIHKINESVEIEDIERLSQIYQKALEYLLGH"
     gene            complement(1724..2812)
                     /gene="hemH"
                     /locus_tag="PK522_08525"
     CDS             complement(1724..2812)
                     /gene="hemH"
                     /locus_tag="PK522_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNC41815.1"
                     /translation="MMLPEPPFQHGTSSKTGVLLINLGTPDAPTAEALKPYLRQFLSD
                     SRIIELPRWIWWFILNGIILNTRPKKSAEKYALVWTEEGSPLKVHTERQTKLLAQALA
                     SSISPVPMVEYAMNIGNPSIAHVLAKMKAAGCDQILVLPLFPQYAASSIGSAMEHVFA
                     ELAKIRNMPAVRTIKHFHDDHGYIAALAQNVQDYWNSHGKPSKLVISFHGVPRKTLDK
                     GDPYHCECQKTGRLLAEALALKPEEYQVCFQSRFGKAKWLTPYTTDILQELGKQKLNR
                     VDVVCPGFVADCLETLEEIAIEGKKTFVEAGGNEFHYIPCLNEHPAWINALTQITQNH
                     LQGWLAPEPTAEEAALCRERATKLGASS"
     gene            complement(2881..3903)
                     /gene="hrcA"
                     /locus_tag="PK522_08530"
     CDS             complement(2881..3903)
                     /gene="hrcA"
                     /locus_tag="PK522_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNC41816.1"
                     /translation="MLNDRAKSLLKTLVERFIVDGEPVGSRSLSKFSGLDLSPATIRN
                     VMVDLEEMGYIASPHTSAGRIPTALGYRFFVDSLLVVKTLESHEVNHLENQLHPDNPS
                     RLINVASQLLSELTRFAGVVVTPKRSGSAVFRHIEFMTLSEKRVLLILVTPEGDVQNR
                     IIFTEASYSQTDLIEASNFLNQHYAGCTLEEIRIRLQTELKQLRHEMMLLMNAAINVS
                     NDVIQESSEAVVVAGEHKLLDVKDLSDNLSNLKQLFELFERKTKLLQLMDLSRQAHGV
                     KVFIGGESNVAALDEVSVITAPYEIEGAIVGTVGVIGPRRMAYERVIPIVDITARLLS
                     SSLSQH"
     gene            4111..4995
                     /locus_tag="PK522_08535"
     CDS             4111..4995
                     /locus_tag="PK522_08535"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNC41817.1"
                     /translation="MSTRFNKIALIGKHKNPEILAPMMKLAEYLASRQFGVIIDRLSA
                     AHLEHSEFPILTLEEIGAEADLAVVIGGDGTMLNIARILAPYNVPLIGVNQGRLGFLT
                     DLTVDTMIETLGEMLDGQFMIEQRVLLSAEVIRQDQVVYKALAFNDIVIHRGISTGMI
                     EFQVRINGEYVYSLRSDGLIVATPTGSTAYALSAGGPILHSSLDLIELVPVCPHTLSN
                     RPIVVGPDAEIEIMLNYNTETKIYSDSHSWFDLGEHDRILVRRFPQVVKLLHAINHSY
                     YRMLREKLGWSEIPHKNH"
     gene            5004..6680
                     /gene="recN"
                     /locus_tag="PK522_08540"
     CDS             5004..6680
                     /gene="recN"
                     /locus_tag="PK522_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNC41818.1"
                     /translation="MLTHLTIQNFVIVERIELSLEPGFTVLTGETGAGKSILIDALML
                     ALGGKSDTNQIRQGCERAEIIAEFNIEHLPDTQRWLQENDLADDSGSCILRRVIEANG
                     RSRHFINGISVALQQLRAIGDQLVDIHGQHAHQSLLRSDAQRALLDGFAGNDALLSQV
                     KAAYQHWHGLNQQRLAWEANQSAFAQEKEQLAWQVDELTSLNFNPQEWQSLQADHQRL
                     SHSASLQAAVEYSIDQLSEGEAALLGQLQTTLTRLRQALDYDEKLQSPYQLLESTSVQ
                     LQEGIHELKHYQKRLDSDPQRLQDVEERIAAIHAMARKYRCDIESLPERLQLAHDRLQ
                     VICGEYSGDNLIEAEASARQHYEAVAQQLTLSRAGAAQSLAKQVTQSMQNLAMAGGIF
                     VINLIPVEQGNANGFEQIEFQVSPHKDTPARPLTKVASGGELSRISLAIQVITSKVSR
                     VPTLIFDEVDAGIGGRIAEMVGTLLKQLGSAHQILCITHLPQVAAMGDQHWLITKIKD
                     AKHAQGISSHIDDLDQQARVEEIARMLGGATITTITRQHAAEMLQKCQNR"
     gene            complement(6692..>7055)
                     /locus_tag="PK522_08545"
     CDS             complement(6692..>7055)
                     /locus_tag="PK522_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNC41819.1"
                     /translation="DGLLALNFVAFFEQGQNTALASVAKTLAQSFAHQLAFISEPDHE
                     FNDFIFIASRQPLTIEQSLLNTQQTIWLKQRRIEIDQRKGIVLTDNFNPLEHLQGNKS
                     EHYRRMMVDMIGTQFFIR"
BASE COUNT         1951 a         1467 c         1667 g         1970 t
ORIGIN      
        1 cgttgccgat gcgtgaatta cattgtctgg atgaagcatt gattcgtgat ttagtaatgc
       61 taaagctgaa aatggtggaa gtgggcgcag aacacgccaa taaacgacct gctgaactct
      121 ctgggggtat gatcaagcga gttgctctag cccgggcgtt agcgcttaat cctgaattac
      181 tttttcttga tgaaccgacg gccggactcg atccttcatt aagtgaaagt attgtggatc
      241 ttgttcgagc gctgagggaa gaactaacgc taaccatcgt catggtgacc catgatctcg
      301 atacgttggc tgcgatttca acccggatcg cagtattggc agatcagcat attgttgcgt
      361 tgggaaagtt acaagaggtt gttcaggttg atcacccgtt tatcaaggat tttttctggg
      421 gtgaacgagg acaaaatgcg ttgcgcaaat atttaattgc tgattagcag ctagcgcttc
      481 gctaaaaatg aatcgtacgg ataaggtcta tcggatttca gattatttaa agtaaagctt
      541 aaagcgaaca taaggaaaat aaatgtcagg tgatacgctt acactcgctc aaatgttgat
      601 ttcacgacgt tcattgacac cacgtgatga cgggtgtcaa aaactgctag aaaaccgttt
      661 gaaagcatta ggatttcaga tcgaaaaact caattttgga gatgttgaga atttgtgggc
      721 gcgtcgtggc acaaaacaac cgttagtgtg ttttgcgggg cacacggatg tcgttcctac
      781 aggacctgtg ggtgagtggg aaagcgatcc gtttgtgccg accatccggg ataatcgatt
      841 atatggacgc ggtgctgccg atatgaaggc ctcattggct gcctttatta cggcgattga
      901 atcgtttttg gaagcgaatc ctgagcctga aggatcgatt gcgttattga ttacctctga
      961 tgaagaaggg cctgccgtcg atggaaccgt caaggttgtc gagattctca aggaacgggg
     1021 tgaatcgatt gatttttgta ttgtcggtga gccgacttgc acggatcaat ttggggacac
     1081 cattaagaat ggccgacgcg gttctttatc tggccattta acgatcaaag ggattcaagg
     1141 gcatattgcg tatccgcact tggccaaaaa tccgatccat ctcgccgcac ctgtcattgc
     1201 tgagctagca caaaccgtgt gggataacgg caatgaatat tttcctccga caacctggca
     1261 tatttccaat atccagggag gtacaggagc aaccaatgtg atacctggcc acatcagttt
     1321 aatatttaat tttcgttttg caacagcgag tacgccagaa tcgctacaaa agaaattttg
     1381 cgaaattctt gacaagcacg gtcttgagta tcagataaca tgggagttat caggtaagcc
     1441 ttatttgaca ccgagaggaa aattggccga tgctttaacg gaagccatta aaactgtaac
     1501 cacgataaca ccacagcttt ctgcaacggg tggaacctca gacgggcgat ttattgcaga
     1561 aatctgtcca caagtcgttg aattcggtcc acgtaacgcg acgatacaca aaatcaatga
     1621 gtcagtagaa atagaagata tcgagcggtt gtcacaaatt tatcagaaag ccttggaata
     1681 tttgctcggt cactgagcca atattctggt ttggctagca tgactagctg cttgcaccca
     1741 atttggtggc gcgttcacga caaagtgcag cttcttcagc agtgggttct ggcgctaacc
     1801 aaccttggag atggttctgt gtgatctggg ttagtgcgtt aatccaggcg ggatgctcat
     1861 tgaggcaagg gatataatga aattcatttc cacctgcttc aacaaaggtt tttttgcctt
     1921 caatggcaat ttcttctaag gtttctaagc agtccgcaac aaatcccggg caaaccacat
     1981 cgactcgatt aagtttttgt ttgccgagtt cttgaagaat atcagtggta taaggtgtta
     2041 gccacttagc cttgccaaag cgtgattgaa aacaaacttg atactcttct ggctttaatg
     2101 ctaaagcttc ggccagtaat cttcccgttt tctgacattc gcaatgataa ggatcgccct
     2161 tgtccaacgt ttttcgtggg acaccatgaa agctaatgac caacttgctc ggcttaccgt
     2221 ggctattcca ataatcctgt acattttgtg ccaaggctgc aatgtagcca tgatcatcat
     2281 ggaagtgctt aatcgtccga acagccggca tattgcgaat tttggccaat tcagcgaaaa
     2341 catgttccat cgcagaaccg atgctactcg ctgcatattg aggaaacagg ggtagcacta
     2401 gaatctgatc acatccagcc gctttcattt ttgctagaac gtgtgcgata gaaggattac
     2461 caatattcat tgcgtattcc accatgggaa ccggagagat ggatgaagct aatgcttggg
     2521 caagtagctt agtttgtcgt tcggtgtgaa ccttgagggg agaaccttcc tcggtccata
     2581 ctaatgcata tttttctgct gatttcttgg ggcgcgtatt gaggataatg ccattcagaa
     2641 tgaaccacca aatccagcga ggcagttcta taatgcgaga atcactcaag aattggcgta
     2701 agtaaggttt tagcgcttct gctgtgggtg catccggtgt tcctaaattg attaataaca
     2761 caccggtttt actcgatgtt ccgtgctgaa aggggggttc tggtagcatc atgatttatt
     2821 cttgtgatgt gttgagtagc aatagtgatt gttttaaagg cgatttatcg tgaaatcaaa
     2881 tcagtgttgc gagaggctgc ttgaaagtaa tcgtgctgtg atatcaacaa taggaataac
     2941 gcgctcataa gccattcgtc tcgggccaat gacacccaca gtgccgacga ttgcgccttc
     3001 gatttcatag ggtgcagtga taacactgac ttcatccaat gctgcaacat tcgattctcc
     3061 cccgatgaaa actttgacac catgtgcctg gcggctgaga tccatcaatt gtaataactt
     3121 ggttttacgt tcaaataatt cgaacaattg ttttagattg gagagattat ccgagagatc
     3181 tttcacgtcg agcaatttgt gctctccagc caccacaacg gcttcgctac tctcttgaat
     3241 gacatcgtta ctgacgttaa tcgcagcatt catgagtagc atcatttcat gacgaagttg
     3301 tttcaattct gtttgcaaac ggatgcgaat ctcctcaagc gtacaacctg cataatgttg
     3361 attcagaaaa ttactggctt caatcaaatc ggtctgacta taactcgctt ctgtaaaaat
     3421 gatacggttt tgtacgtcgc cctctggtgt gaccagaatg agcaatacgc gcttttcaga
     3481 cagcgtcatg aattcgatat ggcggaaaac agcgctaccg gaacgttttg gggtaacgac
     3541 tacacctgca aatcgcgtca attcagataa taattgagaa gcaacattaa tcaatcgaga
     3601 aggattgtct ggatgcaatt gattttcaag gtgattcact tcatggcttt ctaatgtttt
     3661 aacgaccagt aagctatcga caaaaaatcg ataaccgagc gcagtcggaa tgcgtcctgc
     3721 cgaagtatgc gggctcgcaa tataacccat ttcttctagg tcaaccatga cattgcgaat
     3781 cgttgcagga ctaagatcca atcctgaaaa ctttgagagc gaacgcgacc ctactggctc
     3841 tccatcgaca atgaagcgct ccacgagtgt tttgagtaat gattttgcac gatcgtttaa
     3901 catatcagtt gatattttac acgattaaac ttataccgcg cgtaagttaa cccctgctta
     3961 tccatggtat ttttatgtta tccctttgtt tgtgttgtca ccgcattatc cttttacctt
     4021 tccagttgac tacgttaata ttcaaaataa tcacattacc tgtgtcagtg tattaataga
     4081 tatgtattgc cctaatttgt ttcaataaca atgagcactc gttttaataa aattgcatta
     4141 attggcaaac ataaaaatcc agaaattcta gcgccgatga tgaagctcgc ggaatacctt
     4201 gcgagtcgac aatttggcgt cattattgat cgattaagtg cagctcattt ggagcattcc
     4261 gagtttccca ttttgacttt ggaagagatt ggtgccgagg ctgatctggc ggttgtcatt
     4321 ggtggggatg ggacgatgtt gaatattgct cgcatattgg cgccgtataa tgttccattg
     4381 attggggtca accaaggtag attgggtttt ctaaccgatt taaccgtcga caccatgatt
     4441 gaaacactag gagaaatgct tgacggccag tttatgattg agcaacgggt gctgctttca
     4501 gcggaagtca ttcgacagga tcaagtggtt tataaagcac tggcttttaa cgatatcgtt
     4561 attcatcgcg ggataagtac cgggatgatt gaatttcagg tacgcattaa tggtgaatat
     4621 gtttattcat tgcgttcgga tgggttgatc gttgcgaccc caacgggatc gactgcttac
     4681 gcactttcag cagggggacc gatattgcat tccagccttg atttaatcga attggttcca
     4741 gtttgtccgc ataccttgag taatcgtccc attgtcgttg gacctgacgc agagattgag
     4801 atcatgctga attacaatac tgagactaaa atttattcag atagtcattc ttggtttgat
     4861 cttggtgaac atgatcggat attggtaagg cgttttcctc aagtcgtcaa attgctacac
     4921 gcgattaacc atagctatta ccgcatgctg cgtgagaagt tagggtggag tgaaataccg
     4981 cataaaaatc attgattgtc agcatgctga cccatttaac catacaaaat ttcgttatcg
     5041 tcgagcgcat cgaattgagt ctcgagccgg gttttacggt gttgacggga gaaacagggg
     5101 caggaaaatc cattttaatc gatgcattaa tgcttgcatt agggggcaaa agtgatacca
     5161 atcaaatccg gcaaggctgt gaacgtgctg aaatcatcgc tgaattcaat attgagcatt
     5221 tgccggatac tcaacgttgg ttgcaagaaa atgatctggc ggatgattca ggaagttgca
     5281 ttttacgacg cgtcatcgaa gcgaatggac ggtcacgcca ctttataaat ggtatttcag
     5341 ttgctttaca gcaattacgc gcaatcggtg atcaattagt cgatattcat ggacagcatg
     5401 cgcatcaatc gttattacga agtgatgcac agcgcgcttt gctcgatgga tttgctggaa
     5461 atgatgcatt gctgtcacaa gtcaaagcag cttatcagca ctggcatggc ttaaatcaac
     5521 aacgccttgc ttgggaggct aatcaatcag cgtttgcaca agaaaaagag caattggcct
     5581 ggcaagtgga tgagctgact agccttaatt ttaatcccca agaatggcag tcattacaag
     5641 ccgaccacca acgtttatcg catagcgcta gcttgcaagc agccgttgaa tattcgattg
     5701 atcaattatc ggagggagag gctgcgctat tgggacaact tcaaacaaca ctgacccggc
     5761 ttcgtcaagc tttggattac gatgagaaat tgcaatcccc ttatcagtta ctggaatcca
     5821 catcggtgca attgcaagaa gggattcatg agctgaaaca ctatcaaaaa aggctcgact
     5881 cagatccaca gcgtttgcaa gatgttgaag agcgcattgc tgcgattcat gccatggcgc
     5941 gaaaatatcg ctgtgatatc gaatcattgc ctgaacgact tcagttggct catgatcgtt
     6001 tgcaagtgat ctgtggagag tactcgggtg ataatttgat cgaggcggaa gccagtgctc
     6061 gacagcacta tgaagcagtt gcccaacaat tgacactttc acgcgctggg gcagcacaat
     6121 cgcttgcaaa gcaagtgaca caaagcatgc aaaatttggc gatggcgggg gggatttttg
     6181 taatcaatct gatcccagtc gagcagggta atgcaaatgg gtttgaacag atcgagtttc
     6241 aagtatcacc gcataaagat accccagccc gccctttgac caaagtagca tcagggggtg
     6301 agctatctcg aatcagttta gcgatacaag tcattaccag caaggtaagt cgcgttccta
     6361 cactgatatt tgatgaagtc gatgctggaa ttggtggacg tatagccgag atggtaggca
     6421 cattacttaa acaattagga agtgcccatc aaattttatg tattacccat ttaccccagg
     6481 ttgctgcgat gggagatcag cattggctca ttaccaaaat aaaggatgca aaacacgctc
     6541 aaggaatctc aagccatatt gatgacttgg atcagcaagc acgtgttgag gaaattgcgc
     6601 gaatgctggg aggcgcaacg attacaacga ttacccgaca acatgctgca gaaatgctgc
     6661 agaagtgcca aaacaggtaa taattgactt gctagcggat aaaaaattgg gttccgatca
     6721 tgtctaccat catccttcgg taatgttccg atttgttacc ttgtagatgt tccaaaggat
     6781 taaaattatc cgtcagcaca atgcctttac gctgatcgat ttcaatccta cgctgtttta
     6841 accatatcgt ttgctgggta ttcagcaaac tttgctcaat cgtgagtggc tgcctgcttg
     6901 cgataaagat gaaatcatta aactcatgat cgggttccga gataaacgca agttgatgag
     6961 cgaagctttg tgcgagggtt ttcgcaacag aagctagcgc cgtattttgc ccttgttcaa
     7021 agaatgcaac aaaattcagc gctaagagac catct
//
LOCUS       DAOQLJ010000117         7028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_4460_length_7028_cov_6.362111, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000117 DAOQLJ010000000
VERSION     DAOQLJ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4460_length_7028_cov_6.362111"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PK522_08550"
     CDS             <1..547
                     /locus_tag="PK522_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNC41820.1"
                     /translation="FALFRLDLDSELLFVGDAGTTEASRPSRREGFEISTFYMPTPWL
                     MIDLDYALARARFSDSDPSGDHIPGAIRGVGKAAISIDNIGPVFGSLQFRYFGKRSLI
                     EDNSVRSNHTMTLNGQIGYKVSDNVRVIMQAFNLLNTKAHAIDYYYTSRLPGESLGGI
                     NDLHFHPIESRSFRLNLVMNF"
     gene            598..1059
                     /gene="nikR"
                     /locus_tag="PK522_08555"
     CDS             598..1059
                     /gene="nikR"
                     /locus_tag="PK522_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HNC41821.1"
                     /translation="MERFTISMDDQLFQQFDQAAQKRGYDNRSEAIRDLVRDYLETTR
                     LEKDIHGYCIATLSYIFNHHERDLASQVTSAHHHHHDITLSSMHVHMDHDNCLEVVIL
                     RGTIQDVRRFANRVIATNGVRHGKLHIVPIELSQEHHEHSSVPHTHSSPLT"
     gene            complement(1127..2110)
                     /gene="yut"
                     /locus_tag="PK522_08560"
     CDS             complement(1127..2110)
                     /gene="yut"
                     /locus_tag="PK522_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HNC41822.1"
                     /translation="MGWSMAIDNAIPKPLRVMLRGVGQVFFCCNAVTGLIFLIALYVG
                     GITAGLAATVGVVSSTIAAYLLGFSDDDIEAGLYGFNGTLVGPCLFLFLENTPQLWLY
                     VVLASILSTVVLAALMRILQPYNIPASTSPFVLTCWMFMVAVYSFDGFSRGSLLPAPG
                     IPVDIATTSAATMEAWFTNFSQATIEAWYTGITKGIGEVMFADSVIVGILFLVGIAIT
                     SLRGAVMALLGAIVGVAVPVLLGADKTLIELGLYAFNPVLTMMAVGWVFLKPSTGTAL
                     LALLAGILTIICQAGLANFLAPIGLPTLTFPFVLVMWMFLFAASKSKYWKS"
     gene            complement(2214..2849)
                     /gene="ureG"
                     /locus_tag="PK522_08565"
     CDS             complement(2214..2849)
                     /gene="ureG"
                     /locus_tag="PK522_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HNC41823.1"
                     /translation="MNNVHKQSNPLRVGIGGPVGSGKTALCEALSKRMRDHYEMAVIT
                     NDIYTKEDMEILLRANALPPERLMGVETGGCPHTAIREDASINLEAIARMTHDFPNLD
                     LILVESGGDNLASTFSPELSDLTIYVIDVAAGEKIPRKGGPGITRSALLVINKIDLAP
                     YVGASLEIMERDAKKMRGDRPFVFTNMHTGEGVDQVVDFIVKQGLLDEVKH"
     gene            complement(2861..3538)
                     /locus_tag="PK522_08570"
     CDS             complement(2861..3538)
                     /locus_tag="PK522_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HNC41824.1"
                     /translation="MQSLTLLRLLQLASPSLPIGAYTYSQGLESAIEKGTVSNESSAR
                     QWIAESLSIVADFEAPIVWRLLKAFTARDAEQVAYWTESFVAARDTAEFRAETIQMGY
                     SLGKLITDLNVADESLKAILANQSEVPLPTAFACAAVALGIPHEAALLGMLFSLVENQ
                     VLVCVKSVPLGQVSGQSLLLSLHPEIEHAAHHAQRLEDDELSNWAPGLSLLSMQHETQ
                     YSRVYRS"
     gene            complement(3547..4071)
                     /gene="ureE"
                     /locus_tag="PK522_08575"
     CDS             complement(3547..4071)
                     /gene="ureE"
                     /locus_tag="PK522_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HNC41825.1"
                     /translation="MITLNTKIDHAETIYAQLVLPYEKRENSRLRATLASGEEVAIFT
                     ERGTVLRHNDLLKSADGQVVQIVAAHEPTYKITCRTPHDLLRCAFHLGNRHTQTQVGD
                     GFLRILQDTVLKQMLEGLGASIAEENAQFEPESGAYGGGGHHHHGDGHYHAHGPLAPI
                     PARQKIHRPSDTKA"
     gene            complement(4236..5942)
                     /gene="ureC"
                     /locus_tag="PK522_08580"
     CDS             complement(4236..5942)
                     /gene="ureC"
                     /locus_tag="PK522_08580"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HNC41826.1"
                     /translation="MSVKISRQTYAEMMGPTTGDRVRLADTELFIEIEKDFTTYGEEV
                     KFGGGKVIRDGMGQSQRNHADVMDTVITNAVIVDHWGIVKADIGLKNGKIAAIGKAGN
                     PDIQPNVTMAIGSATEIIAGENMIVTAGGVDTHIHFISPQQAEDAMMNGITTMLGGGT
                     GPAVGTAATTCTPGPWHMHSMLRASDGIVMNTGFFGKGNTSLPTPLEEQILAGGCGLK
                     LHEDWGTTYAAIDNCLAVADKYDVQVAIHTDTINEGGYLENTIAAMKDRTIHTFHTEG
                     AGGGHAPDIIAVVGLDNVLPSSTNPTRPYTVNTLDEHLDMLMVCHHLHANIAEDLAFA
                     ESRIRKETIAAEDILHDMGAISMMSSDSQAMGRIGEVVLRTWQTAHKMKIQRGTLQED
                     NSRNDNFRVKRYVAKYTINPAITHGIGHAIGSVEVGKYADLVLWRPGFFGVKPSMILK
                     GGMIAASLMGDPNASIPTPQPVHYRYMFGGYGGGIKTSCFTFASQAALSAGLVDQLKL
                     DKNIVAVKNTRNLRKKDMIHNSATPKMEVDPETYEVRADGQLLTCGAEDVLPMAQRYF
                     LF"
     gene            complement(5975..6295)
                     /locus_tag="PK522_08585"
     CDS             complement(5975..6295)
                     /locus_tag="PK522_08585"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HNC41827.1"
                     /translation="MRLPLIPGEVFTEAGEIELNVGRKTKTLTVSNGGDRPIQIGSHF
                     HFYEVNSAMKFDREAAYGMRLNIMAGTAVRFEPGQERTVELVELAGDRVVYGFNQKVM
                     GKLK"
     gene            complement(6318..6620)
                     /locus_tag="PK522_08590"
     CDS             complement(6318..6620)
                     /locus_tag="PK522_08590"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HNC41828.1"
                     /translation="MDLTPREKDKLQIFTAGLLAERRKARGLRLNYPEAVALITCAVL
                     EGARDGRTVAELMTAGQKVLSRDEVMEGVPEMIPDIQVEATFPDGTKLVTVHNPIV"
     gene            complement(6708..>7028)
                     /locus_tag="PK522_08595"
     CDS             complement(6708..>7028)
                     /locus_tag="PK522_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HNC41829.1"
                     /translation="LEQICLKGENATMKGALALSGYTVCATLLLTGKTIAQPLIDLAR
                     LEAERLTNKTGQVGISQLKSVVVVRYLGHSSEVARQVMLSVWGLFRPEVLGYPAIVPR
                     MWTT"
BASE COUNT         1985 a         1736 c         1420 g         1887 t
ORIGIN      
        1 atttgcgctc ttccgcttag atttggattc agagttattg ttcgttggtg atgcaggcac
       61 gactgaagcc agtcgaccca gccgacgcga agggtttgaa atttcgacct tttatatgcc
      121 aaccccttgg ctaatgatcg atctcgatta tgcgctggcc agagcacgct ttagtgatag
      181 cgatcccagt ggcgaccata ttccaggagc cattcgggga gtcggaaaag cagctatctc
      241 gatcgataat atcgggcctg tttttggtag tctgcaattt cgctattttg gcaaacggtc
      301 gctcatcgaa gataatagcg tacgctcaaa ccacacgatg acgttgaatg gtcaaatcgg
      361 ttataaagtt agcgataatg ttcgcgtaat catgcaagcc tttaatttat tgaataccaa
      421 agcgcatgcc atcgattatt actatacttc tcgactacca ggagaatcgt tagggggaat
      481 caatgattta cattttcatc cgatcgaatc tcgatcattt cgccttaatc tagtgatgaa
      541 tttttaattt taccgcttat tactaattgt tatgtgtcca ttcaggagaa attaattatg
      601 gaacgcttta cgatttcaat ggatgatcag ctcttccaac agtttgatca ggctgctcaa
      661 aaacgaggct atgataatcg atcagaagct attcgcgatt tggttcgtga ttatctggaa
      721 accactcggt tagaaaagga tattcatggc tactgcatcg caacattgag ttacattttt
      781 aatcatcatg agcgggattt agccagtcaa gtaacctcag cacaccatca ccatcacgat
      841 attaccttat cgagcatgca cgttcatatg gaccatgata attgtctaga agtcgtgatt
      901 ctcaggggta cgatccaaga tgtcaggcgg tttgcaaacc gagtcatcgc taccaatggg
      961 gtacggcatg gaaagctaca tatcgtaccg atagaactct cccaggaaca tcatgaacat
     1021 tcttcggttc cacacactca cagtagccca ttgacctaat catattcaac caataaaaaa
     1081 acccgccaga aggcgggtca ttcaatgaat tcagttgaag ctagattcaa cttttccagt
     1141 atttcgattt actagcagca aataaaaaca tccacattac caaaacaaaa ggaaaggtaa
     1201 gggttggtaa tccaatcggt gcaagaaaat ttgctaatcc agcttgacaa ataatcgtca
     1261 agattccagc taacaaagcc agaagcgctg ttccagtaga gggtttgaga aaaacccaac
     1321 ccacagccat catggtcaga acaggattaa atgcatataa acccagttca atcaaggttt
     1381 tatccgcacc cagtaagact ggtactgcaa caccgacaat ggcacctaat aatgccatca
     1441 ctgcaccacg cagcgaagtg atcgcaatac cgaccaggaa tagaataccc acaatgacac
     1501 tgtcagcaaa catcacttca cctatcccct tggtaatccc tgtataccaa gcttcaattg
     1561 ttgcttggct aaaattggta aaccaggcct ccatggtcgc tgcggatgtt gttgcgatat
     1621 ctactggaat tcctggggct ggtagtaaag aaccccgaga aaagccatca aaagaataaa
     1681 ccgcaaccat gaacatccag catgtcagga caaacggaga tgttgaagca ggaatattgt
     1741 acggctgcaa aatacgcatt agtgctgcca acaccacggt ggacagaatc gatgcaagca
     1801 cgacatacag ccaaagttga ggtgtgttct caaggaataa gaacaagcag ggcccaacga
     1861 gcgttccatt gaaaccataa agcccagctt cgatatcatc atcagaaaaa cctaataaat
     1921 aggctgcaat cgtactacta accacaccaa ccgttgccgc caggcctgca gtaattccgc
     1981 ctacatacag tgcgatcaag aaaataagcc cagtaaccgc attgcagcaa aaaaagactt
     2041 gcccaacccc tctgagcatt actctcagtg gttttggaat tgcattgtca atagccatag
     2101 accatcccat agtgactaac tcctcaagta gtattttgta aaataaaact gcgataactc
     2161 gactagaacc ttaactcagc ttactcagtt gtattgaatt tctacatagt agattaatgc
     2221 ttcacttcat ccagcaaacc ttgcttcaca atgaaatcga caacttgatc aacaccctcc
     2281 cctgtatgca tattggtaaa cacaaaagga cgatctcctc gcattttctt agcatcacgt
     2341 tccataattt ctagactagc cccaacataa ggcgccaaat cgattttatt gatgaccagt
     2401 aaagcagagc gtgtgatacc aggcccccct ttgcggggta ttttttcgcc ggcggcaaca
     2461 tcgatgacgt agatggttaa atccgataac tcaggactaa acgttgaagc caggttatct
     2521 ccacccgatt caactaagat tagatcgagg tttggaaaat catgcgtcat gcgcgcgatt
     2581 gcctctaaat taatcgatgc atcttctcga atcgccgtat gcggacatcc gcctgtttct
     2641 acgcccatta agcgttccgg gggtaaggca ttggcgcgga gcaaaatctc catatcctct
     2701 ttggtataga tatcattggt gatgacggcc atttcataat ggtctcgcat gcgtttgctt
     2761 aacgcttcac atagcgctgt tttacccgat ccaaccgggc cgcctattcc aactcgcagt
     2821 gggtttgatt gtttgtgtac gttgttcata gtgatataga ttaagatcga taaactcggc
     2881 tatattgcgt ttcgtgttgc atggaaagca atgaaagtcc aggcgcccaa tttgatagct
     2941 catcatcctc aagtcgttgt gcatgatggg cagcatgttc aatctctggg tgcaatgaca
     3001 ggagaaggct ctgcccagaa acctgtccaa gcggtactga tttgacgcaa acgagtactt
     3061 gattttcaac caaagaaaat agcataccca ataacgccgc ttcatgcgga ataccgagtg
     3121 caacagcagc acaagcaaac gctgtaggta aaggtacttc tgattgattc gccagtatag
     3181 cttttaaaga ttcatcagca acattaagat ccgtgatcaa cttaccgagg gaatagccca
     3241 tttgtatcgt ttctgcacga aattcagccg tatctctcgc tgcaacaaaa ctttcggtcc
     3301 agtaggcaac ttgctccgca tcacgggcag taaatgcctt gagtagacgc cacacgatgg
     3361 gtgcttcaaa atcagccaca atactcagcg attcagcaat ccattgacgt gcagagctct
     3421 cattggacac cgttcccttc tcaatcgctg attccagtcc ttgtgaatag gtataggcac
     3481 caatgggaag cgatgggctc gccaactgaa gcagacgcaa tagtgttaga gattgcaaaa
     3541 tagctcctat gctttggtat cagatggtcg atgaatcttt tgtcgcgcag gaataggggc
     3601 taatggacca tgtgcatgat aatggccgtc accatgatgg tgatgaccgc cacccccata
     3661 agcacctgat tcaggctcaa attgtgcatt ctcttcggca atcgaggcgc ccagcccctc
     3721 aagcatttgc tttaataccg tatcttggag aatacgtaga aagccatcac cgacttgtgt
     3781 ctgtgtatgg cgattcccca aatggaatgc gcaacgtaat aaatcatggg gcgtgcggca
     3841 ggttatttta taagtaggct catgggcagc aacaatctga accacttgcc catcagcact
     3901 tttgagtaaa tcattatgac gaagaacggt gccacgttcc gtaaaaatgg ctacttcttc
     3961 tccggaagca agcgtagctc ttaatcgact attctcacgc ttttcataag gtagaaccaa
     4021 ttgagcgtaa atagtctcag catgatcgat tttagtattt aatgtaatca tctgttttat
     4081 tctggtttat agataagtta tagatgatta ctatcacagc aatctaggta ttaacgacgg
     4141 ctgtgatagt cactgccaaa tagcgcagtc cattacgcta tgactaaacc taataggtta
     4201 gctaatgcat gaactgcgca acttgaacgc taccgctaga ataagaaata ccgttgtgcc
     4261 atcggcaata cgtcttctgc gccacaggtg agtaattgcc cgtcagcacg aacttcatag
     4321 gtttccggat caacttccat cttcggtgtt gcgctattgt gaatcatatc tttcttacgc
     4381 aaattacgcg tatttttgac cgcaacaata tttttatcca gcttgagttg atcgactaaa
     4441 ccagcactca acgcggcttg cgatgcaaac gtaaagcacg atgttttaat tcccccacca
     4501 tacccaccga acatataacg ataatgaacg ggctgtggtg ttgggatcga tgcattagga
     4561 tcgcccatca atgaagctgc aatcatgcca cctttcagga tcatagatgg tttgacgcca
     4621 aagaaaccag gtcgccagag cactaagtca gcatatttac caacttcgac tgaaccgatc
     4681 gcatgcccaa tcccatgggt aattgcaggg ttgatggtgt atttcgcaac ataacgtttc
     4741 acgcggaaat tatcattcct tgaattatct tcttgcaatg taccgcgttg tattttcatt
     4801 ttgtgcgctg tttgccaagt ccgcaatacc acttcaccaa tccgtcccat cgcttgagaa
     4861 tccgatgaca tcatcgaaat agcgcccatg tcatgcagaa tatcttctgc tgcaatggtt
     4921 tccttacgaa tacgagactc agcaaaagcc aaatcttccg caatattggc atgcaaatga
     4981 tggcaaacca tcaacatatc taaatgctca tccagggtat tcacggtata aggacgagtt
     5041 ggattcgttg aagaaggcag aacattatcg agccccacaa ccgcgataat atcgggtgca
     5101 tgccccccac ccgcaccttc agtatggaag gtatgaatgg tacggtcttt cattgcagca
     5161 atggtattct ctaaatagcc accttcgttg atcgtatccg tatggatcgc aacttgaaca
     5221 tcatacttat ctgctactgc caggcaatta tcaatcgcag cataagtcgt tccccagtcc
     5281 tcatgcagct tgagaccaca accaccggct aaaatttgtt cttcaagcgg ggtgggcaaa
     5341 ctggtatttc ctttaccgaa gaaacccgta ttcatcacga ttccatcaga agctctcagc
     5401 atcgaatgca tatgccaagg gccaggagta caagtggttg ccgcagtacc gacagcggga
     5461 cctgttccac cgcctagcat cgtggtaatg ccattcatca tggcatcttc agcttgttgc
     5521 ggagaaatga agtggatatg cgtatcaaca ccacctgcag tgacgatcat attttcacca
     5581 gcgatgatct cagttgccga accgatcgcc atcgtgacat tcggttgaat atctgggtta
     5641 cctgctttac cgattgcggc aatcttgcca ttcttaaggc caatatccgc tttgacaatt
     5701 ccccaatgat caacaatgac cgcattggta atcacggtat ccatcacatc cgcatggtta
     5761 cgctgcgatt gccccattcc atcccgtatt actttaccgc ccccaaactt tacttcctca
     5821 ccataggtcg taaaatcttt ttcaatctca ataaaaagct ctgtgtcagc aagccgtaca
     5881 cggtcgcctg ttgtcgggcc catcatttct gcataggttt gacgggaaat ttttacgctc
     5941 atttttttgc tcctctaaaa ataatccggt acgactactt aagtttgccc atcacttttt
     6001 gattgaatcc atagacaact cgatctcctg ccaactccac gagttcaacg gttctctctt
     6061 gtccaggctc aaaacggacg gctgtgccag ccataatatt gaggcgcatg ccataagcag
     6121 cttctcgatc aaatttcatc gcagagttaa cttcataaaa atgaaaatgt gatccaatct
     6181 gaatggggcg gtctcctcca tttgaaaccg taagtgtttt ggtttttctg ccaacattca
     6241 actcaatttc cccagcttcg gtaaatactt cacctggtat taaaggtaat ctcataacga
     6301 tctcctatct tgatcaatta cacaattgga ttatggaccg taaccaactt ggttccatcc
     6361 ggaaacgttg cttctacttg gatatcagga atcatttctg gaaccccttc catcacttca
     6421 tcccgagaca atactttttg ccccgccgtc atcaattcag caacggtacg cccatctcta
     6481 gcgccttcaa gtactgcgca ggttattaat gccactgctt ccggataatt gagccttaag
     6541 ccgcgcgctt ttcttctttc agcaagtaac cctgccgtaa atatttgtaa tttgtctttc
     6601 tctcttggtg ttagatccat ctcatttctc ctgagtaaaa aatattttca gatgatcggc
     6661 actacttaac attaaacaat caacagcttc cctacgtaaa accgagctca tgtcgtccac
     6721 atccgcggca caattgctgg ataacccagt acttctggtc gaaataaccc ccatacgctt
     6781 aacatgacct gacgggccac ttcacttgag tgaccaaggt aacggactac aaccacggat
     6841 ttaagctggg aaatacccac ctgcccagtt ttattggtta atctttccgc ttccaatcgt
     6901 gccagatcaa ttaaaggctg cgcaatggtt ttaccagtca gtaataacgt agcgcagacg
     6961 gtatagcctg aaagtgccaa agcccccttc atcgttgcgt tctcgccttt taaacagatc
     7021 tgctctag
//
LOCUS       DAOQLJ010000118         7011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_4479_length_7011_cov_6.269120, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000118 DAOQLJ010000000
VERSION     DAOQLJ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 7011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7011
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4479_length_7011_cov_6.269120"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..511)
                     /gene="groES"
                     /locus_tag="PK522_08600"
     CDS             complement(221..511)
                     /gene="groES"
                     /locus_tag="PK522_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNC41830.1"
                     /translation="MNIRPLHDRVIVKRLEEERKTASGIVIPDSATEKPDQGEILAVG
                     KGKAGDDGKIRPLEVKVGDKVLFGKYSGQAVKIKGEEYLVMREEDIMGVIEG"
     gene            678..1991
                     /gene="bioA"
                     /locus_tag="PK522_08605"
     CDS             678..1991
                     /gene="bioA"
                     /locus_tag="PK522_08605"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HNC41831.1"
                     /translation="MNDKNLSIRSFKHIWHPCTQMKHHETSPLVPIEKAQGIWLYDTN
                     GNRYMDAISSWWVNLFGHCHPYINDALVDQLNKIEHVMFAGFTHQPAVELAERLAQIT
                     PGNLDHCFFASDGASATEIALKMSFHYWQLYGKTDKVNFISLKNGYHGETLGSLSVTE
                     IPFFRQTYAPLLRNTEYMPSPDWRNIIGNESHLEFALKAASELEIFLSKHHTQIAALI
                     LEPIVQAAGGMGMYHPIYLQRARTICDKYQVHLIADEIAVGFGRTGKMFACEHAEIVP
                     DLLCLAKGLTGGYLPLSVVMTTDKLYQAFYQDEISKAFVHSHSHTGNALACRASLATL
                     DLIEQYNIIEANQAKIDYLNQLASPLQSHPKVKNFRNRGMIWAFEVETNDSKFSNNFF
                     QKALEKQLLLRPLGKTVYFMPPYIIKEREIEHLVSNVLQIIDMQS"
     gene            2009..3415
                     /locus_tag="PK522_08610"
     CDS             2009..3415
                     /locus_tag="PK522_08610"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC41832.1"
                     /translation="MAQLTFLGGTNEVTGSCYLVETEETRFLVDCGMFQGGSEAEIKN
                     RSAFSFDPETIDFVLLTHAHIDHSGLLPRLSARGFRGPVYTTGATIDLLKVMLKDSAY
                     IQEKEVEWKNKILQRKQRVSEYENAPLYTVTQAESFLKQLYSIAYDEKVFPHPTIQCC
                     FRDAGHILGSAIVEVWIKTQSKQKKLVFSGDIGQPGHPVVQDPTLIHQADVLLVESTY
                     GNRLHRSMVDTIDELTYAVNDTLLRKGGNIIVPAFSVGRTQELLFLLIDLHRQNKLGK
                     MDIYVDSPMAKAATDITLQHSQLLDKEAHETLQWFNKEGNPLKINFTENVEDSIALNA
                     IDHGAIIISASGMCDAGRIKYHLKYNLPRPECTILITGFQAAGTLGRRLVDGAKIVRI
                     FGQEIVVRSDLYTIGGLSAHADQAGILNWLKAFKTPPNHTYIVHGEPSNANALANIIK
                     QQLNWPVSIPERFATVTL"
     gene            complement(3436..4677)
                     /locus_tag="PK522_08615"
     CDS             complement(3436..4677)
                     /locus_tag="PK522_08615"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HNC41833.1"
                     /translation="MKKTFLLDRLGWLIIVSLVLLSISHSKADAVTDWNKWAGNIVVD
                     AEIGPLPAERALAIVQAAVYESVNAITRRYSTSDIELLPPNPDASIEAAIAEANRVTL
                     MQLAPNQQASIEKTYQTAISAISEGSAKRAGIELGAKVAQAILAKRTQDGAADKESYR
                     PNTSAGVYIPTVIPEAPQWMKRTPWLMTNPAQFRPASPPELGSDIWARDYNEVKALGR
                     FDSQVRTSEQTEIARFWEEVMPPIYHGIVRSVATQPGREITQNARLFAAVTQASDDAL
                     IAVFDAKYHYGLWRPVTAIRNGDIDGNEATERDATWLPFISTPMHPEYPCAHCIVSAA
                     VGTVLEAEIGNNPSPILTTVSKAANNATRSWRNVHDFMQEVINARIYDGVHYRNSGEV
                     GSNMGRQIAQLAIKKYEVGEK"
     gene            complement(4720..5616)
                     /gene="hemF"
                     /locus_tag="PK522_08620"
     CDS             complement(4720..5616)
                     /gene="hemF"
                     /locus_tag="PK522_08620"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HNC41834.1"
                     /translation="MNMIEVKAYLADLQNRIVAGLEAIDGKSFQRDGWERPDGGGGLS
                     CVIEEGNVLERGGVNFSYVFGHGLPASATAARPELAGRSFEAAGVSLVLHPRNPYAPT
                     VHMNVRFFEAKKEGADPVWWFGGGMDLTPYYGFEEDAIHFHQTCKTALDPIGSDYYPR
                     FKKWCDEYFFLKHRKEPRGIGGVFFDDFNQPDFSTCFKLTQRVGDHFLPAYAPILERR
                     LNVPYGEQERDFQAYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSLPPIVKWRYNW
                     TPQAGSPESKLYTDFLIGKNWI"
     gene            5759..6934
                     /gene="aroC"
                     /locus_tag="PK522_08625"
     CDS             5759..6934
                     /gene="aroC"
                     /locus_tag="PK522_08625"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNC41835.1"
                     /translation="MSGNTLGKLFCVTSFGESHGPAIGCVVDGCPPGLELSEADIQYE
                     LDRRKPGTSRHVTQRREADVVEILSGVFEGKTTGTPIGLLIRNEDQRSKDYSKISDTF
                     RPGHADYVYWQKYGIRDYRGGGRASARETAVRVAAGAIAKKWLNKTYGTLIRGYLSQL
                     GAISIPFKQWYDVNQNPFFVSDNDFVPQLEGYMDSLRKSGDSVGAKISVVAEGVPVGW
                     GEPVYDRLDAEIAYAMMSINAVKGVEIGSGFACVIQKGTEHSDEMTPNGFLTNNAGGI
                     LGGISTGQPITVNVAIKPTSSIRLGRRSIDKQGNPVIVETHGRHDPCVGIRATPIVEA
                     MLALLLIDHALRHRAQNADVYCSTPKIAGSIQQSNQTSVPVEDINLDHTENPDAEEA"
BASE COUNT         2048 a         1582 c         1404 g         1977 t
ORIGIN      
        1 acaccatctt tagtaatagt cggtgcacca taggaacgct ccagcaccac attacgacct
       61 tttggaccca aggttacctt gactgcgtct gcaagaatgt taaccccgtg taccaaatta
      121 tgacgtgcta catcaccaaa tctaacttct tttgctgcca taattcttct cctaaattac
      181 tgttgcatga tcaaatttca aattaaaagg attaaatacc ttaaccttca ataacaccca
      241 tgatgtcttc ttcacgcata acaaggtatt cttcaccttt gatttttaca gcttgaccgg
      301 aatattttcc aaacaaaact ttgtcaccaa cttttacttc caaagggcga attttcccat
      361 catccccagc ttttcccttt ccaacagcta aaatttcacc ttgatctggt ttctctgttg
      421 cagaatctgg aataacaatt cccgaagccg tcttgcgttc ttcttctagc cgcttgacaa
      481 taacacgatc gtgtaagggg cgaatattca tagttgattc tcctaataat gattaaaaaa
      541 catgaacata cttaaaatca aatcaattca atcaaaactc atgctggttt tgtagcagta
      601 ttcgctattt taaagtaatt agcactcaac actgacgagt gctaataata tgatctattc
      661 acaggaattt caagtcgatg aatgataaaa atttatcaat acgtagtttt aagcatatct
      721 ggcatccgtg cactcagatg aaacaccatg agacatctcc ccttgtacct atcgaaaagg
      781 ctcaaggtat ctggctttat gatacaaatg ggaatcgtta tatggatgca attagttctt
      841 ggtgggtaaa tctttttggg cattgccacc cttatatcaa tgatgcgcta gttgatcaat
      901 tgaataaaat tgaacatgtc atgttcgccg gttttactca tcaaccggct gttgagctag
      961 cagaaagatt ggctcagatt acgccaggta acttagatca ttgttttttt gctagcgatg
     1021 gggcatcagc tactgaaatt gctctaaaaa tgagctttca ctattggcaa ttatatggaa
     1081 aaactgacaa agtcaatttc atcagcttga aaaatggtta tcacggggaa acgctcggtt
     1141 cattatcggt caccgaaatt ccatttttta gacaaactta tgcgccactg cttcgaaaca
     1201 ctgaatatat gccttcaccc gattggcgaa atattatcgg caatgaatca cacctagaat
     1261 tcgcattaaa agcagcctct gaattagaga tttttctttc caagcaccat acacaaattg
     1321 ccgcacttat tctcgaaccc attgttcagg cagctggcgg aatgggtatg tatcatccta
     1381 tttacttaca acgggctaga actatttgtg ataaatatca agttcattta atcgccgatg
     1441 aaattgcagt tgggtttggt cgtacaggaa aaatgtttgc gtgcgaacat gctgaaattg
     1501 ttccagattt attatgtttg gcgaaaggat taacgggggg ctatctgcca ctttccgttg
     1561 tcatgacaac tgataagcta taccaggctt tttatcaaga cgaaatctca aaagcgtttg
     1621 ttcattccca ttctcatact ggaaatgccc tggcatgccg tgcatccctt gccacactgg
     1681 atttgatcga acaatacaac ataattgaag cgaatcaagc gaaaattgat tacctcaatc
     1741 aacttgcttc accactgcag tcccacccta aagtaaaaaa ctttcgtaat cgtggcatga
     1801 tctgggcttt tgaagtcgaa acgaatgact ccaagttctc gaataatttt ttccagaaag
     1861 cactcgagaa gcagctactg cttcgaccac ttggaaaaac agtatacttt atgccaccat
     1921 acatcattaa agaacgtgaa attgaacatt tagtaagcaa tgttctacaa attattgaca
     1981 tgcaatcgta gtaaaaatag gaaatacctt ggcacaactt actttcttag gtggcacgaa
     2041 tgaggtaaca ggttcctgtt acctcgtaga aactgaggaa actcgatttc tagttgattg
     2101 tggaatgttt caagggggaa gtgaggcaga gattaaaaat agatctgcat tcagcttcga
     2161 tcctgaaaca attgatttcg tgctcctcac ccatgcgcat attgatcatt ctggattact
     2221 accacgatta agcgctcgag ggtttagagg accggtctat acgactgggg ccacaatcga
     2281 tctactcaag gttatgctaa aagatagcgc ctatattcaa gaaaaagaag tggaatggaa
     2341 aaataaaatc ctacaaagaa aacaacgtgt ttctgaatat gaaaatgcgc ctttatacac
     2401 agttacccaa gcagaaagct ttcttaaaca gttgtacagt atagcttatg atgaaaaagt
     2461 ttttcctcat ccaacgatac agtgctgctt tcgtgatgcg gggcatatct taggatccgc
     2521 gattgtcgag gtctggataa agacccaatc aaagcaaaag aaattagtct tctcagggga
     2581 cattggacaa ccaggacatc ccgttgttca ggatccaact ctgattcatc aagcagatgt
     2641 tctactcgtt gaatcaactt acggtaatcg acttcatcga tctatggtag ataccatcga
     2701 tgagcttacc tatgccgtta acgatacgct attaagaaaa ggcggtaaca tcatcgtgcc
     2761 tgctttttct gttggaagaa ctcaagaact ccttttctta ctcatcgatt tacatcgaca
     2821 aaataagtta ggaaaaatgg atatttatgt tgattcgcca atggcaaaag ctgccacaga
     2881 tattacactt cagcatagcc aattactgga taaagaagcg catgaaaccc tacagtggtt
     2941 caataaagaa ggaaacccac taaaaatcaa cttcaccgag aatgttgaag attccatcgc
     3001 gctcaatgcg attgatcacg gggctatcat catttcagcg agtggtatgt gcgatgcggg
     3061 tcgtatcaaa tatcatctta aatataacct tcctagacct gaatgcacga tactgatcac
     3121 cggttttcaa gccgccggaa cattaggacg aagattagtc gatggtgcca aaattgtacg
     3181 gatttttggt caagaaattg ttgttcgatc agatctctac accattggcg gactttcagc
     3241 gcacgcagat caagctggga ttctaaattg gctcaaagca tttaaaacac ctcccaacca
     3301 tacctacatc gttcatggtg aaccgagtaa tgctaatgcc ctagccaata tcataaaaca
     3361 acaactcaac tggcccgtta gtattccaga aagattcgcc acagtaacgc tttgatcgtt
     3421 caagatgata caaacctact tttcacctac ctcatatttc tttattgcaa gctgagcaat
     3481 ttgccgaccc atatttgagc ccacttctcc cgaattacga taatgcaccc catcataaat
     3541 ccgtgcatta atcacttctt gcataaaatc gtgtacatta cgccagctcc tggttgcatt
     3601 attcgctgcc ttactcaccg tagtcagtat tggggacgga ttatttccta tttccgcttc
     3661 caatacggtt ccaactgcag cagaaacgat gcaatgcgca cacggatact ctggatgcat
     3721 cggcgtactg ataaatggta accaggtagc atctcgctct gttgcttcat tcccatcgat
     3781 atcgccgttc cgaatggctg ttactggcct ccagagtcca tagtgatatt tagcatcgaa
     3841 aacagcaatg agcgcatcat cggaagcctg agttacggca gcaaataaac gtgcattttg
     3901 tgtaatttct ctccctggtt gggttgctac ggaacgtacg atgccatgat aaattggggg
     3961 cataacttcc tcccagaaac gagcaatctc tgtctgttca ctcgtcctga cctgactatc
     4021 aaatcgacca agggctttta cttcattata atctcgcgcc caaatatcgc ttcctaactc
     4081 aggtggggac gccggcctaa attgcgctgg attcgtcatc agccaaggcg ttcgcttcat
     4141 ccattgtggt gcctctggaa taacagtggg tatataaaca cctgcactag tattgggccg
     4201 ataagactct ttgtcagcag cgccatcttg ggttcgcttt gctaatatcg cttgcgctac
     4261 ttttgcacct aactcgatac ccgctctttt tgctgaaccc tcgctaatag cagaaatagc
     4321 agtctgatat gttttttcaa ttgaagcttg ctgattaggt gctagctgca ttaacgtaac
     4381 tcgattagct tctgcaatcg cagcttcaat tgatgcatcg gggttaggtg gtagcaattc
     4441 aatatcactt gtagagtaac gacgtgtgat ggcattgact gattcataga ctgctgcttg
     4501 aacaattgct aatgcgcgtt cagcgggtaa aggcccaatt tcagcatcga caacgatatt
     4561 acccgcccat ttattccagt ctgttacagc atcagccttt gaatgggata tagacaataa
     4621 tactaagcta acaataatca accaacccaa tcgatctaat aaaaatgttt tcttcatcta
     4681 gaaacctcca agaaagtagt ttaaatcaat agttctcaat taaatccagt tcttgccaat
     4741 caagaaatct gtatatagct tggattcggg gctccctgct tgtggcgtcc agttatagcg
     4801 ccattttaca atcggcggca gtgacatcag aatggactca gttcgcccac ccgtctgcaa
     4861 gccaaataaa gttccccgat cccagaccaa gttgaattcc acataacgac cacgtcgata
     4921 agcctgaaaa tcccgttctt gttcaccgta agggacattt aagcgcctct ccagaattgg
     4981 agcatacgca ggtaaaaaat gatctcctac tcgctgagtc aacttaaagc aggttgaaaa
     5041 atcgggttga ttgaaatcat caaagaaaac accgcctatt cctcttggct cttttcgatg
     5101 tttgagaaaa aaatattcgt cacaccattt cttaaaccgt ggatagtagt ctgatccaat
     5161 gggatcaagc gctgttttac aggtttgatg gaaatgaatt gcatcctctt caaagccata
     5221 ataaggcgtt aaatccatcc ccccaccaaa ccaccatacg ggatcagcac cttccttttt
     5281 cgcttcaaaa aagcgtacat tcatatggac tgtaggggca tatggattac gtggatgcaa
     5341 aacgagtgac actcccgccg cttcgaaaga acgacctgcc aactcggggc gtgctgccgt
     5401 cgcagaggca ggtaagccat ggccaaagac atacgagaaa ttaacaccgc cccgctctag
     5461 tacattgcct tcttcaatga cacagctcag ccctccgcct ccatcaggcc tttcccatcc
     5521 atcacgttga aatgacttgc catcgattgc ttctagacct gctacaattc tattttgcaa
     5581 atcagccaga tatgctttga cttcaatcat attcattctt tattccccct caatctacta
     5641 cactgcatta gttaatttct ctattattcc tagggtaatt ctaacctcag ctcgaaagct
     5701 agggaagaaa tttggttgct atgttagact tactttactt atttcataaa taaaaggtat
     5761 gtcaggcaat acattaggaa aattgttttg cgttacgtcg tttggtgaat cgcatggccc
     5821 tgcaatcggc tgtgtagtag acggttgtcc acctggactc gagctatctg aggcagatat
     5881 tcaatatgaa cttgatcgac gcaaacctgg aacctcgcgt catgtaaccc aacgtcgcga
     5941 agcagatgtt gttgaaatac tctctggtgt ttttgaagga aaaacaacgg ggacaccgat
     6001 cgggctccta attcgaaatg aagatcagcg aagtaaagac tatagcaaaa taagcgacac
     6061 tttccgtcct ggccacgctg attatgtgta ttggcaaaaa tatggtatcc gtgattaccg
     6121 aggtggcggt cgagcatctg ctcgcgaaac agcggtaaga gtggcggcag gtgcgatagc
     6181 aaagaagtgg ttgaataaaa cctatggcac gctgattcgt ggctacctgt cacagcttgg
     6241 agcaattagc atccctttca agcaatggta tgatgtcaat cagaatccct tttttgtttc
     6301 agacaatgac tttgtccctc aacttgaagg ttatatggat agtctacgca agtcaggtga
     6361 ttcagttggc gccaagatta gtgttgttgc tgaaggtgtg ccagtcggtt ggggagaacc
     6421 cgtctatgat cggctcgatg ctgaaattgc ctatgcgatg atgagtatca atgcagttaa
     6481 gggtgtcgaa atcggctcag gattcgcatg cgttattcaa aaaggaactg agcactctga
     6541 tgaaatgacg ccaaatggct ttttgacaaa caacgctggt ggcattctgg gaggcatttc
     6601 gactggccag ccgataacag ttaatgttgc gattaaacca acatcaagta tccggttggg
     6661 aagaagatcc attgataaac aaggcaatcc tgtcattgtt gagacccacg ggcgacatga
     6721 tccttgcgtg ggaattcgtg cgacaccgat tgtagaagca atgctggcat tgttgcttat
     6781 tgatcatgcg ctacgccacc gtgcacaaaa tgctgatgtc tattgcagta cacccaagat
     6841 agcaggcagt attcagcaaa gcaatcaaac ttctgtccca gtagaggata taaatctcga
     6901 ccatactgaa aacccagatg ctgaggaagc gtgatcattt tctactggtt ttacttttta
     6961 gtcggtcctg caaaattaac ttaaccgtgg cggaggtttt gtatacaaga a
//
LOCUS       DAOQLJ010000119         6972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_4519_length_6972_cov_5.647680, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000119 DAOQLJ010000000
VERSION     DAOQLJ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4519_length_6972_cov_5.647680"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PK522_08630"
     CDS             complement(<1..866)
                     /locus_tag="PK522_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC41836.1"
                     /translation="MKAVYFDKGGSADVLHYGDIATPNDCGENQVLVRIKAIGINPID
                     CKLRAAPERFPVTFPVIPGCDGAGIVEAVGTKVSHFKSGDEVYFSQPGFKGRQGTYAE
                     YTLVDASLLALKPRTLSFEQAVAAPLVFITAWEALHDRARIMHDQIVLIHAGAGGVGH
                     AAIQLAKQAGARVITSVSNEEKAAFVKQLGADLIINYRTQDVIAEVMRWTDSNGVDIA
                     FDTVGPTVLHSCFRCVKPYGDVVTILQATTDTDWSEARKRNVRFSHELMLSPVLLELE
                     QAKQHQGEILKQ"
     gene            complement(890..1390)
                     /locus_tag="PK522_08635"
     CDS             complement(890..1390)
                     /locus_tag="PK522_08635"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNC41837.1"
                     /translation="MNPPIYLLAAVARNRVIGCDNKMPWHLPEDLKHFKQLTLGHIVI
                     MGRKTYESIGKPLPERINIVITRQPMFSAANLIVVASIEAALQACTPYPDKKRFVIGG
                     SEIYQQTLPMSQRLYLTEIQKDFVGNRYFPEFDRNEWSEAAREIHHQGTADGLEYHFV
                     ILDRRS"
     gene            complement(1387..2181)
                     /locus_tag="PK522_08640"
     CDS             complement(1387..2181)
                     /locus_tag="PK522_08640"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNC41838.1"
                     /translation="MHQYLELMRSILKQGHQKSDRTGTGTLSMFGYQMRFDLAAGFPL
                     VTTKKCHLKSIIYELLWFLRGDTNIHYLKQNGVSIWDEWADERGNLGPVYGHQWRSWT
                     VADGGAIDQISQVIEQIKKTPDSRRLIVSAWNVGDLDKMHLAPCHVLFQFYVVDGKLS
                     CQLYQRSADVFLGVPFNIASYALLTLMIAQVCNLKPGEFIHTFGDAHLYLNHLEQAQE
                     QLKRQPRQLPTMHIDPSVHRIFDFDYHHFELKDYDPYPAIKAPVAV"
     gene            2267..3736
                     /locus_tag="PK522_08645"
     CDS             2267..3736
                     /locus_tag="PK522_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HNC41839.1"
                     /translation="MLATLIQNNTSINESVIASHVLVLLPKLEKLPNKYDVLGEETLK
                     ALLARRQMEFDRLSETPVCANLNNGVLCAWVMVDLEQPIFDQQTTIRKALQLLLDEEP
                     SSVHLAVNGNAHQKQSLARLAVYVAWVNGAVLPVRKNRPDRKPLDTIYLYGYEEADSF
                     TLLRAQAEGNLLARGLTVLPPNELTPKTYREQIKKLAQNESWNYQEYDLATLRKMGAG
                     AFVAVAQGSDTEDAAIVHLQRHSSKKHCKTIALVGKGICFDTGGHNLKSARYMHGMHE
                     DMNGSAVALGILLAATRTDLPFNIDCWLAIAQNHISPRAYKQNDVITAVNGTTIEIIH
                     TDAEGRLVLADTLALAEKLKPDLIIDFATLTGSMFTALGSRYSGIFTNRDYLAEHALA
                     AGKSSGERVCVFPMDKDYETELESNIADTKQCKLDSDFDHILAARFLSRFVNDIPWLH
                     MDLSACNHKNGLGAVGTDVTGFGVAWAAAFLEKLPKAEA"
     gene            3828..4649
                     /gene="amrB"
                     /locus_tag="PK522_08650"
     CDS             3828..4649
                     /gene="amrB"
                     /locus_tag="PK522_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNC41840.1"
                     /translation="MLTKRLPAVAGLFYPADCRTLGEEVNHYLRLTPISEIIPKALIV
                     PHAGYIYSGAIAAVGYNTLRSIAARIRRVVVLGPAHRVALRGLALPGVDSFMTPLGQV
                     EIDTDAVERIKNLPQVIVNAQAHESEHALEVQIPFLQTMLGEFKLLPLVVGMASTEEV
                     AEVLDCVWGGEETLIVISSDLSHYLPYESAQNKDSKTALSILQLRPLVNHEQACGAIP
                     INGLLAAARQHHLVAHLLDLRNSGDTAGSRDQVVGYGAFAFSEPHSPLARQYHVA"
     gene            4639..5217
                     /gene="amrA"
                     /locus_tag="PK522_08655"
     CDS             4639..5217
                     /gene="amrA"
                     /locus_tag="PK522_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNC41841.1"
                     /translation="MLLDQGNVLLAVGRAAIAQALNLDFQYPVDESASWLQAWGASFV
                     TLTQHQELRGCIGSLEAYRPLVADVRSNAVSAALHDPRFLPLSADEFETIAIEVSILS
                     LTQRIAALDEADAIRQMRPGIDGIVLEYGRYRSTFLPQVWDELEQPQQFLAMLKRKAG
                     LPVDFWAADLKLARYTVSKWRETDGMEDYRHG"
     gene            5210..6304
                     /gene="amrS"
                     /locus_tag="PK522_08660"
     CDS             5210..6304
                     /gene="amrS"
                     /locus_tag="PK522_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNC41842.1"
                     /translation="MDESISLEEQYPAKYWHQLQDSRVQCDLCPRDCKLHEGQRGACF
                     VRGRAGDRMVLTTYGRSSGFCVDPIEKKPLNHFYPGSSVLSFGTAGCNLACNFCQNWD
                     ISKSRSFDKLADQASPETIALTAKRLGCKSVAFTYNDPVIFAEYAMDVADACHAQGIN
                     TVAVTAGYIHAEPRADFFAKMDAANVDLKAFTESFYVKQTGSHIQPVLDTLKYLKHET
                     NVWLELTTLLIPGLNDSAQELQALSTWVANELGSEVPLHFSAFHPDYKMRDIEATPLS
                     KLIEARRIAMAAGLRYVYTGNIHHPEGDTTYCSSCGRVLIERDWYEIKHYHLTANDHC
                     PDCGTAIAGRFGKSAEHFGRQRIPVLLGYQ"
     gene            6301..6945
                     /locus_tag="PK522_08665"
     CDS             6301..6945
                     /locus_tag="PK522_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNC41843.1"
                     /translation="MMTTISITDDTARLEGELMIPPGAQGIVVFVHGSGSSRFSPRNN
                     YVAKIIRDRGLGTLLFDLLTRQEDQNYTTRFEISLLTQRLLAATAWLGAETKTSKLKI
                     GYFGASTGAAAALQAAAKLGRKIAAVVSRGGRPDLAGVSALEKVMSPTLLLVGRADDG
                     VIELNEQAYSRLQCEKQLVLIPGATHLFEEPGTLEQVANQAADWFAHYLTDTAA"
BASE COUNT         1863 a         1462 c         1750 g         1897 t
ORIGIN      
        1 cattgcttta atatttctcc ttgatgttgc ttggcttgct ctaactccag taaaaccggt
       61 gaaagcatga gttcatgact aaagcgcaca ttgcgtttac gcgcctcgct ccaatcggta
      121 tcagtcgtgg cttgtagaat agtgacgaca tcgccgtaag gtttgacgca acggaagcaa
      181 ctgtgcagga ctgttggacc aacggtatcg aaggcaatgt ctacgccgtt gctatcagtc
      241 cagcgcatga cttcagcaat aacgtcttgg gtacggtagt taataatcaa atcggcgccg
      301 agctgtttca cgaaagcggc tttttcctca ttgctgacgg aggtgatgac gcgagcacca
      361 gcctgtttgg ctaattgaat tgcagcatga ccaacaccac cggcgccagc atgaattaaa
      421 acgatttgat cgtgcatgat ccttgcccga tcatgtagtg cctcccacgc ggtgataaat
      481 actaatggcg cggcgaccgc ctgttcaaaa gataacgtac gcggcttcaa cgcaagtaaa
      541 ctggcatcga ctaaggtata ctcagcatag gttccttggc gacctttaaa accgggctgt
      601 gaaaaataaa cttcatcgcc tgacttgaaa tggctgacct tggtgccgac ggcttctaca
      661 attcctgcac catcgcaacc gggtatcacg ggaaaagtta cggggaaacg ttcgggtgct
      721 gcacggagtt tgcagtcgat gggattgatt ccaatcgctt tgatacgaac cagtacttga
      781 ttctcaccgc agtcgtttgg ggttgcgata tcaccgtagt gcaatacgtc ggctgaccct
      841 cctttgtcaa aataaactgc tttcataatt ggatttccat aaaatgactt taacttcttc
      901 gatcaaggat tacaaaatgg tactcgagtc catctgccgt gccttgatgg tggatttcac
      961 gcgctgcttc agaccattca ttgcggtcga attcgggaaa gtagcgattt cccacaaaat
     1021 ctttctgaat ctcggtaagg tagaggcgtt gactcatagg aagtgtttgc tggtaaattt
     1081 ctgaaccacc aatcacaaag cgttttttat cggggtaggg tgtgcaggct tgcaatgcag
     1141 cttcgatgga tgcaacgacg atcaagttag cagccgagaa catgggttgt cgcgtaatca
     1201 cgatattgat tcgctctggt aagggtttgc cgatcgattc ataggttttt cgccccatga
     1261 tgacgatatg ccccagtgtc agttgcttaa aatgtttcaa gtcttctgga agatgccatg
     1321 gcatcttatt atcgcaacca atgacccgat tacgggcaac ggcggctaat aagtaaatag
     1381 ggggattcat actgccacgg gcgctttaat cgcaggatag ggatcatagt ctttcaactc
     1441 aaaatggtga tagtcaaagt caaaaatacg atgaactgat gggtcaatgt gcatggtcgg
     1501 caattgtctt ggttgtcgtt tgagctgttc ttgcgcttgt tccagatgat taaggtaaag
     1561 atgggcatcg ccaaaggtat gaatgaattc gccaggtttt aggttgcaca cttgagcaat
     1621 cattagcgtc aacaacgcat aagaagcaat attgaaagga acgcctaaaa aaacatccgc
     1681 actgcgttga tagagttggc aagataactt gccatccaca acataaaact ggaaaagtac
     1741 atggcaaggc gctaagtgca ttttatccaa gtcacccacg ttccaagcgg atacaatgag
     1801 acgccgcgaa tcgggtgttt ttttgatttg ttcgatcact tgcgagattt gatcaatcgc
     1861 gcctccatcg gcaactgtcc atgaacgcca ttgatggcca tagaccgggc cgagattgcc
     1921 tcgctcatcc gcccactcat cccagattga aacgccattt tgcttcagat agtggatatt
     1981 ggtatcgccg cgcagaaacc ataagagctc atagataatg gatttcaagt gacacttttt
     2041 cgtggtgacc aaaggaaatc ctgctgcgag atcaaatcgc atttggtagc caaacatgga
     2101 caaggtgccg gttccagttc tgtccgattt ctggtggcct tgcttaagga tagaacgcat
     2161 taattcaaga tattggtgca taggtggtga gtatacttct gagaagaaaa aaccaggtag
     2221 ccccttataa agctataata aggacaactt aatgatagga attctgatgc ttgcaacact
     2281 catacaaaat aatacatcca ttaacgaatc tgtaatagct tctcatgttt tagtactatt
     2341 gccaaaactc gaaaaattac ctaataaata tgatgtattg ggagaagaaa cactaaaggc
     2401 actgcttgct cggcgtcaaa tggagtttga tcgactgagt gaaacgccgg tatgcgctaa
     2461 tctgaacaat ggtgtgttgt gtgcctgggt tatggtggat ttggaacaac caatattcga
     2521 tcaacaaacc acgattcgaa aagccttaca actattgctg gatgaagagc caagctcggt
     2581 tcacctggca gtaaatggaa atgctcacca gaaacaatca ctggctaggt tagcggtgta
     2641 tgttgcttgg gtgaatggtg ctgttttacc ggtacgcaaa aataggcctg atcgaaaacc
     2701 actggatacg atttatttat atgggtacga agaagcggat agcttcacgt tgctccgtgc
     2761 tcaagcagaa gggaatttgt tagcgagagg gttaacggtt ttaccgccga atgaattaac
     2821 acccaaaacg tatcgcgagc agattaagaa attagcccaa aatgaaagtt ggaactacca
     2881 agaatatgac ctagcgactc tccgtaaaat gggcgcaggt gcatttgtgg cggttgctca
     2941 gggtagcgat accgaagatg ctgcaatcgt acatttgcag cgccacagtt caaagaaaca
     3001 ttgcaaaacg atcgcattgg tcggtaaggg gatatgcttt gatacgggag ggcacaacct
     3061 aaaaagtgcg cggtatatgc acggtatgca tgaggatatg aatggctcgg ctgtagcgtt
     3121 aggcatttta ttagcagcga cccggactga tttaccgttt aacatcgatt gctggttagc
     3181 aattgctcaa aatcacatca gcccccgcgc ctataagcaa aatgatgtga ttaccgcagt
     3241 caatgggacg acaatcgaaa tcattcatac cgatgcagaa ggacgcttgg ttctcgcaga
     3301 taccttagct ttggccgaaa aactcaaacc tgatttaatt atcgattttg caacattgac
     3361 ggggagtatg tttaccgcgc taggatcacg ctatagcggt attttcacga atcgcgatta
     3421 tttagcggaa cacgcattag cggctggtaa atcaagcggg gaacgtgtgt gtgtatttcc
     3481 gatggataag gattatgaaa cagagttgga aagtaatatt gctgacacca aacaatgtaa
     3541 attagacagc gattttgatc atattctcgc tgctcgcttt ttgagtcgtt ttgtcaacga
     3601 tataccatgg ctccatatgg atctttcagc ctgcaatcat aaaaatggtt tgggcgctgt
     3661 cgggaccgat gtgactggat ttggagtggc ttgggcagcc gcgttcctgg agaaattacc
     3721 gaaagctgag gcgtagataa cttcggtttt attctaaagt tttattattg cccttgtttt
     3781 tctgatacat attcccatat tgatatcgtt caatacaggg aaggtttatg ttgacaaaac
     3841 gattgcctgc tgtggcaggc ttgttttatc cagcggattg tcgcacatta ggtgaagaag
     3901 ttaaccacta tttaaggttg acacctatct cagaaataat acctaaggct ttgattgttc
     3961 cccacgcagg ttatatttac tcaggcgcga ttgcagcagt cggttacaac acgttgcgct
     4021 caattgcagc gcgcattcgg cgcgttgtag tgttagggcc agcgcatcgg gttgctttgc
     4081 gaggattagc attaccggga gtcgatagtt ttatgacgcc tttagggcag gttgagattg
     4141 atactgacgc agttgagcgt ataaaaaact taccccaagt tatcgtcaat gcccaagcgc
     4201 atgagtcgga gcatgcattg gaagtgcaga tccctttttt acaaacgatg cttggtgagt
     4261 ttaaattatt accattagtg gttgggatgg catccaccga ggaggttgct gaggtattgg
     4321 attgtgtctg gggtggagaa gaaacactga ttgtaatcag ctcagatctt tctcattact
     4381 taccttatga gtcggcccag aataaagaca gtaaaacagc gctatcaatt cttcagttac
     4441 gacccctggt taatcatgag caagcatgcg gcgcgatccc gatcaacggc ctgcttgcag
     4501 ctgcgcgtca acatcatttg gtagcacatc tattggattt gcgaaattcg ggcgataccg
     4561 ctgggtcgcg cgaccaagtt gttggctatg gtgcttttgc attctccgaa ccacattctc
     4621 cattagcgag gcagtatcat gttgcttgat caagggaacg tgttattagc agttggacgt
     4681 gctgcgatcg cacaggcatt gaatctcgat tttcaatacc ctgttgacga gtcagcgtct
     4741 tggttgcaag cctggggtgc gagttttgtt acgctgactc agcatcaaga attgcgcggg
     4801 tgtattggtt cgctcgaagc ctatcgacct ctagtagcag atgttagaag taacgcggta
     4861 tcggccgcat tgcatgatcc ccgtttttta cctttgagtg cagacgagtt tgaaacgatt
     4921 gcgattgagg tttcaatttt atctctaacg cagcgaatcg ctgctctgga tgaagcggat
     4981 gcgataagac aaatgagacc gggtatcgat gggatcgtgc ttgagtatgg gcgctaccgc
     5041 agtacttttt taccccaggt ttgggatgaa cttgaacaac cccagcaatt tttagcgatg
     5101 ctaaaacgca aagcgggctt accggttgat ttctgggctg ctgatttaaa gcttgctcgt
     5161 tatacggtta gcaaatggcg tgaaactgat ggcatggagg attatcggca tggatgagtc
     5221 cattagcctt gaggaacaat atccagctaa atattggcat caattgcaag atagccgtgt
     5281 tcagtgcgat ctgtgtcctc gggattgtaa attacatgaa gggcagcgtg gtgcttgttt
     5341 tgtcagagga agagctgggg atcggatggt gctgacgacc tatggacgat catctggctt
     5401 ctgtgtggat cccattgaga aaaaaccgct taaccatttt tatccgggca gtagtgtcct
     5461 atcgtttggt actgcaggtt gtaacctggc ttgcaacttt tgccagaatt gggatatttc
     5521 taagtcgcgt agttttgata aattagctga tcaagcaagt cctgaaacca ttgcgctaac
     5581 cgctaaacgc ctgggttgta aaagtgttgc atttacctac aatgatccag tgatttttgc
     5641 tgaatatgca atggatgtcg cggatgcttg tcatgcacag ggcatcaata cggttgcggt
     5701 tacggcaggt tatatccacg ctgaacccag agctgatttt tttgccaaga tggatgcggc
     5761 caatgtcgat ctcaaagcgt ttactgaatc cttctacgtg aaacaaactg gttcccatat
     5821 ccaacctgtg cttgatacat tgaaatatct caaacacgaa acgaatgtgt ggcttgagct
     5881 aaccacttta ctgataccag gattgaacga ttcggcgcaa gaactccagg cattgagcac
     5941 atgggtagcc aatgaattgg gtagcgaagt ccccttgcat ttctcggctt ttcacccgga
     6001 ttacaaaatg agagatattg aggcaactcc gctcagtaag ttaattgagg cgagacgaat
     6061 tgcgatggcg gctggcctcc gctatgttta taccggaaat atccatcacc ctgaaggcga
     6121 cacgacgtat tgctcatcgt gtggtcgcgt gttaattgag cgggattggt atgaaattaa
     6181 gcactatcat ttaactgcaa atgatcattg ccccgattgt ggtactgcaa ttgctgggcg
     6241 tttcggaaaa tctgctgaac attttggtcg gcagcgcatc cctgttttat taggatatca
     6301 atgatgacga cgatatcgat taccgatgat acagcacgat tagagggtga attaatgatt
     6361 cctccaggcg cgcaagggat cgtggtgttt gtgcatggca gtggtagcag ccgatttagt
     6421 ccgcgtaata attatgtggc aaaaatcatt cgagatcgtg ggttaggaac attgctgttt
     6481 gatctgttga ctcgacagga ggaccagaat tatacaaccc gttttgagat cagcttgttg
     6541 acgcaacgtt tgcttgcggc aactgcttgg cttggagcag aaactaaaac cagcaagcta
     6601 aaaatcggat actttggcgc cagtacgggt gctgctgcag ctttacaagc ggctgccaag
     6661 ctcggaagaa aaattgcagc cgtggtatca cgaggaggac gtcctgatct tgctggtgtg
     6721 tcagcgttag aaaaagtgat gtcacctacc ttacttttag ttggcagggc ggacgatggg
     6781 gtaatcgaat taaacgagca ggcctattca cggttacagt gtgaaaagca actagtatta
     6841 ataccgggag caactcattt gtttgaggaa cctgggacct tagaacaagt ggcaaatcag
     6901 gctgcagatt ggttcgctca ttatttaacc gataccgcag catgagatta atggcgactg
     6961 agctttaagg at
//
LOCUS       DAOQLJ010000120         6777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_4714_length_6777_cov_5.245611, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000120 DAOQLJ010000000
VERSION     DAOQLJ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4714_length_6777_cov_5.245611"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1598)
                     /locus_tag="PK522_08670"
     CDS             complement(<1..1598)
                     /locus_tag="PK522_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41844.1"
                     /translation="MAWGRLALIQAIGESEATLQKVAEALGFESKFARQPQMPEQAPI
                     TPDPGGDSAPTMLTTEALPTEKPHAFFLRVNRMETLSPHNVENRGYPYYLDDPALKLD
                     ATQPSQGTYRFAPPPPLLSMSRLAPLLHQGLGQIKTTSRIDFSRMVRHIAQGKFIRRL
                     PRIKQQRWPQQLQIIVDPHVSLEPYWSDFAFIITALKKRLGTERVTALRFDEATLSES
                     QMQATYWPTQPQDRWFVWQLSASDVPLLILSDLGMVTSDANVRYQWRQWITQLRSHAG
                     PILTLSPALQSPQGRQLCALIKPHPLHDRVRLPRHPARKGFELAQPKAETLSNVLTWL
                     SILPIIDTGLLRRLRTEMQWGGSELESLIWNHACFSQTSLGLRMPDSSEAQQYRAKYQ
                     QQFAQTPQAQRFWQIVHDHHAQGFEGLRQLERLSRSISEHEHDDAAHCYFQRVCATIR
                     QTSPVSAHAQALQAQCRTVLLSLPDHLFLSDQQDVAYALYAMAYQQEIQAGQWPESLK
                     PGFEPARLQWLLNEAQAQQRVAWQV"
     gene            complement(1604..2626)
                     /locus_tag="PK522_08675"
     CDS             complement(1604..2626)
                     /locus_tag="PK522_08675"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNC41845.1"
                     /translation="MTQFLKLEHFLPPNFILSKPDKQLYHIFDEADCYALWAAYAAGR
                     PLLVRGKPGTGKSQMAKAIAEQLGWAFVSEVIRGSTELSDLHWHFDAIGRLSEAQAMS
                     FKPQGSTDDWENRANPLRFLSPGAFWWAYDWQSAEQQYQACMTKLHPAPEPEQPESLA
                     ASQQPHGVVLLIDEIDKAEPDLPNGLLETLGQYQFTVPYLNQQAAQSELKNPIQAEPA
                     RLLIVITTNEERELPSAFVRRCLVHTLKMEESAEQINGIEKRVAWLIARAQLHFGSQI
                     SEDVYRKAAELLWQDRTSGNYLRYPPGLAEYIDLLKALRGLEPGEQARRLEKIASYTL
                     KKEVTE"
     gene            complement(2639..4105)
                     /locus_tag="PK522_08680"
     CDS             complement(2639..4105)
                     /locus_tag="PK522_08680"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNC41846.1"
                     /translation="MGEQNTTIKELAGRVPIQAYLSYRHCEETKQARDLLKKHCEAYK
                     IELIYDEEQLKIGDSITAFMDEIASAQCIFIFLTETYFDSAFTLYELIKINECCRDRK
                     TNKPFIVPIKISKSIHGFSASDAKNGHWLGDKNQKCRDDLTEKLGSQVEDHDQAWQRI
                     AEAFPEMLNPFLDKIYPSVEEDASVLETRVNNVYAEIATVIQKSKDKLTQDIKAKITR
                     LLNNRKPIFLNQLSSDLGFASSDNETIARYLAENGDVKGTIKIMTQTLKKQREVLRSV
                     NLEKWRESHAIAEQLCGWLLIKTIDPVWWFNHEFKASKIGKTFASDIFLEERAFVEII
                     ISRDLFQPAKYKRLAETGKVQPFTDNLSIDFIFDANVGALEESFLINLYRDLFKSTGA
                     EGTTAQLKEDIKVQLDTIITEDRKCIYYVTNEATMMSLKSIQGIEEWLQQLEGKLRFI
                     YLPISDEKVLKQAQSPYPEQKALLFQIADLLKIRDEMI"
     gene            4368..6185
                     /locus_tag="PK522_08685"
     CDS             4368..6185
                     /locus_tag="PK522_08685"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding and patatin-like
                     phospholipase domain-containing protein"
                     /protein_id="HNC41847.1"
                     /translation="MNIHGAADKMKALFLSGMTTSMLKLADIPFFNALSPHTLAHLQA
                     TIPVRHYKQGSVIARAGDKGQFFQAIAEGAVWVQQADRYHRKSGIVLGPGQVFGEMSL
                     FSGMPISATLVASKDTKTYCFNSNQFLHLLESVPQLHVNLTNLLIERLRRRTHHEGYA
                     PNLVVLAYETESTLSQKLVERLIDGIHHYSAGSRLFSGLQNPISDAADPELHSKFNAW
                     RESAPNGQLLVLRVRPAELPHLSRYFEPKDVVLHIIDTTTNYIEHFNSADQVGVADFA
                     RVYLGERDQTYCDLQPWAYQLMPAALEKSSTSPNSWQEPFNSTLDKIARYLTFKEVGI
                     AMSSGAALGFAHLGVLDAMAQCGINYDVLCGSSMGGIVALTVAHLGSIDQAIQQVREQ
                     LGSNRKIKDPSLLPRGSLFIGNKIAAAAKATFGDCTFADLKHPAAVVAADLVTHERII
                     FDRGLVAPAVLATSAIPGFFPPVANQSRLMVDGSVVSLVPVDVLSNRRCGLRIAINVN
                     TKPFASGNDVITGFDGLLQKTQKPLGLKDVLSASWGLLGSWRSSSEALRAEIVINPQT
                     PVESGYNFNEFEKLVDCGRAAMHDRIESIQESIKLMLRA"
     gene            complement(6444..>6777)
                     /locus_tag="PK522_08690"
     CDS             complement(6444..>6777)
                     /locus_tag="PK522_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNC41848.1"
                     /translation="ILDEMEESGKLAITALAFIRGRSLSNVMFIIDEAQNLTPHEIKT
                     IITRAGESTKIVFTGDIHQIDTPYLDEQSNGLTYIIDRLKGHPLFAHIRLEKGERSEL
                     ANLANDLL"
BASE COUNT         1643 a         1600 c         1604 g         1930 t
ORIGIN      
        1 acctgccaag ctacacgctg ttgggcttgc gcttcgttta gcaaccactg caagcgtgcg
       61 ggttcaaaac ccggtttcaa cgattctggc cactgccctg cctggatttc ttgttgatac
      121 gccatagcat ataacgcata agcaacgtcc tgttgatcac tcaggaacag atgatcgggt
      181 agcgacagga gcacagtgcg gcactgcgct tgcaaagctt gcgcgtgcgc tgaaacgggt
      241 gaggtttgcc gtattgtggc acacacgcgt tggaaataac aatgggctgc atcatcgtgc
      301 tcatgctcgc taatactacg acttaacctt tccaactggc gtagtccttc aaagccttgc
      361 gcatggtgat cgtgcacgat ctgccagaag cgctgagcct ggggtgtctg ggcaaattgc
      421 tgctgatact tggcgcggta ttgctgtgct tcactcgaat ccggcatgcg caaacccaag
      481 ctggtctggc tgaaacaagc atggttccaa atcaagcttt ccaattcgct gccaccccat
      541 tgcatctcgg tacgcaagcg ccgcaagaga cccgtatcga taatcggtag tatcgacaac
      601 cacgtcagta cgttgcttaa agtttctgct ttcggttgtg cgagctcgaa tccttttcgg
      661 gcggggtgac gcggtaaacg cacgcgatca tgcaagggat gcggtttaat aagtgcgcat
      721 aactgccggc cttgcgggga ttgcagcgcg ggactcagcg tgagtatcgg ccctgcatgc
      781 gaacggagtt gtgttatcca ttgccgccac tgatagcgca cattggcatc gcttgttacc
      841 atgcccagat cgctcaaaat caacaatggc acgtcggacg ccgacaattg ccagacaaac
      901 caacgatcct gcggttgcgt cggccagtaa gttgcctgca tctgcgattc actcagcgtc
      961 gcttcatcaa agcgcaacgc agtgacgcgc tcggtaccca atcgtttctt aagcgcggtg
     1021 ataatgaaag caaaatcaga ccagtagggt tctaaactca cgtggggatc gacaatgatt
     1081 tgcaactgtt gcggccaacg ctgctgcttg atgcgcggca atcgacggat gaatttacct
     1141 tgtgcgatgt gacgcaccat ccgtgagaaa tcgattcgac tggttgtttt gatttgcccc
     1201 agaccctgat gtaacaaagg cgccaagcgt gacatcgata ataacggggg cggtggcgcg
     1261 aaacgatacg ttccttgcga tggctgggtc gcatcgagtt ttaacgcagg atcatccaga
     1321 tagtacggat agccacgatt ttccacgtta tgcggcgaca gcgtttccat gcggttaacg
     1381 cgcaaaaaga aggcatgggg tttttccgtc ggcagtgctt cggttgttag catggttggg
     1441 gcgctatctc cccctggatc tggtgtgatt ggcgcttgct caggcatttg cggttggcgt
     1501 gcaaacttgg actcaaaacc cagggcttca gctactttct gtaatgtggc ttcactctca
     1561 ccgatcgctt gtatgagcgc tagtcgcccc catgccatgg cgtttactcc gtcacttctt
     1621 ttttcagcgt gtaactcgcg attttctcta gacggcgagc ttgctcgcct ggttccagtc
     1681 cacgtaacgc tttgagcaaa tcgatgtatt cggcgagccc cggtggataa cgtaaataat
     1741 tccctgaggt gcgatcttgc cacaatagct cagccgcttt gcgatataca tcctcactga
     1801 tttgtgaccc aaaatgtaat tgggcacgcg cgatgagcca ggctactcgc ttttcaatgc
     1861 cattgatctg ttccgctgat tcttccattt tcaacgtatg aaccaaacaa cggcgcacaa
     1921 aagcgctcgg taattcacgt tcttcattgg tggtgatcac aatcagtaat cgcgctggct
     1981 cagcttggat cgggttctta agttcgcttt gagcagcttg ttgattgaga tacggcacgg
     2041 tgaattggta ttgcccaagg gtttccagca agccattggg tagatccggt tcggctttat
     2101 cgatttcatc gatcaacagc actacgccat ggggctgctg ggaagccgct aagctttctg
     2161 gctgctccgg ctccggtgca gggtgcaatt tagtcataca ggcttgatac tgttgttcgg
     2221 ctgattgcca atcataggcc caccagaatg caccgggaga caagaaacgc agcgggttgg
     2281 cgcgattttc ccagtcatcg gtgcttccct gcggtttgaa actcatcgct tgcgcttcac
     2341 tcagacgacc aatggcatcg aaatgccagt gcagatcgct caattcggtg ctaccgcgaa
     2401 tcacttcact gacaaaggcc caaccgagct gttcggcaat ggctttggcc atttggctct
     2461 tccctgtgcc gggcttgccg cgaactagca gtggtcgccc tgccgcatag gctgcccata
     2521 acgcatagca atccgcttca tcaaagatat ggtaaagctg tttgtcgggt ttcgatagga
     2581 tgaaattagg aggtaggaag tgttcgagtt tgagaaattg ggtcataggt ttaaattttt
     2641 aaatcatttc atctctgatt tttaatagat cggctatttg aaaaagcaat gctttctgct
     2701 caggataagg cgattgcgct tgcttcaata ctttttcatc ggatattggc aaatatataa
     2761 accgtagttt tccttccaac tgttgcaacc actcttcaat cccctgaata gattttaaac
     2821 tcatcatcgt tgcttcattg gttacatagt aaatacattt acgatcttca gtgataatcg
     2881 tgtcaagctg aaccttaata tcctctttta attgtgctgt agtcccttcc gcaccggtag
     2941 acttaaacaa atctcgatac agatttatca aaaaactctc ttctaatgcg ccaacgttcg
     3001 catcgaaaat aaaatctata ctgaggttgt cagtaaacgg ttgtaccttc cctgtttcag
     3061 ctaacctctt atatttcgcg ggctgaaata aatctctgga aataataatt tcaacaaaag
     3121 ccctctcttc caagaaaata tcgctcgcaa aagttttacc gattttgctc gctttgaatt
     3181 cgtgattaaa ccaccaaact ggatcaatcg ttttaatcag taaccatcca cacaattgtt
     3241 cagcaatcgc atgactttca cgccattttt cgagattaac actccgtagg acctctcttt
     3301 gctttttaag cgtttgggtc attatcttaa ttgtgccttt tacgtctcca ttctcggcca
     3361 gatatcgagc aatcgtttca ttatcgctgc tggcgaatcc taaatccgaa ctgagctgat
     3421 tcaggaaaat aggctttctg ttatttagaa gccgtgtaat ttttgcttta atgtcttgag
     3481 tgagtttatc ttttgatttt tgtattaccg ttgcaatctc tgcatagaca ttattaacac
     3541 gggtttccag aaccgatgca tcctcttcca ctgaaggata tattttgtct aaaaacgggt
     3601 taagcatttc tggaaatgcc tcagcaattc gttgccatgc ttgatcatga tcttctactt
     3661 gtgatccaag tttttcagtt agatcatcac ggcacttttg atttttgtcg cctaaccagt
     3721 gaccattttt cgcatctgat gctgaaaagc catgtattga ttttgatatt tttattggta
     3781 cgatgaatgg cttattcgtc ttacgatccc tgcagcattc attgatttta attagttcgt
     3841 acaaggtaaa cgcagaatca aaataggttt cagtgagaaa aataaatata cattgtgctg
     3901 acgctatttc gtccatgaaa gccgtaatac tatcgccgat cttgagctgc tcttcatcat
     3961 aaattaattc gattttgtag gcttcgcagt gtttctttaa taaatcacgg gcttgtttgg
     4021 tttcttcgca gtggcggtag gataaataag cttgaatggg cactctgccc gctaattctt
     4081 tgatagttgt gttttgctca cccatgtttt gcctgtttta atacaacgat catctgatag
     4141 tgaaaattct accaccgtca ttgcacaaaa gtggggttgc ttgcaccaat atgtcataag
     4201 acaatttagg catagatagc caatttggct atttttcgca gtgtacggtt ttagtttcat
     4261 caaaaaatac agggtgttcg tttcgttatc agtgcgtttg ccaagagaac tgttagaata
     4321 caatgaggag taaagcgttg taagggctgc ggcaagcgct ggaatcgatg aatattcacg
     4381 gtgctgctga caaaatgaaa gctttgtttc tttcagggat gacgacttcc atgctaaaac
     4441 ttgcggatat tccatttttc aatgcgttat cgcctcatac gttagcgcat ctacaggcca
     4501 cgattccagt gcgccattac aagcaaggca gtgtcattgc acgcgctgga gataaaggcc
     4561 aatttttcca ggcgattgcc gaaggtgccg tgtgggtaca acaagcagat cgctatcatc
     4621 gcaaaagtgg cattgtctta gggccgggtc aggtatttgg tgagatgtcg ctgttttcag
     4681 gcatgcctat ttctgcgacg ctagtcgctt ccaaagatac taaaacctat tgtttcaaca
     4741 gtaatcaatt tctgcatttg ctggaaagcg taccgcaact acatgtcaat ctaacgaatt
     4801 tattaatcga gcgcttacgg cggcgtaccc atcatgaagg ctatgccccg aacttggtgg
     4861 tgctggctta tgaaacagaa tcgacgcttt cgcaaaaatt agtggagcgg ttgatcgatg
     4921 gaattcatca ctattcggcg ggttcaaggc tattttcagg cttgcaaaac ccaatcagcg
     4981 acgcggctga tcctgaatta cactccaagt ttaatgcttg gcgtgagtct gcccccaatg
     5041 gtcaattact cgtgctcaga gttcgcccag cggagttgcc ccacctcagc cgttattttg
     5101 agcctaaaga tgtagtgcta cacatcatcg ataccacgac gaattacata gaacacttca
     5161 atagcgccga tcaagtggga gtcgctgatt ttgcacgggt ttatttaggg gaacgggatc
     5221 aaacttattg cgatttgcag ccttgggctt atcaattaat gccagcagcg ctcgaaaaaa
     5281 gcagcacctc accgaattcc tggcaagagc cgttcaattc aaccctggat aagattgcac
     5341 gctatttgac gttcaaagaa gtcggtatcg cgatgagttc aggtgctgcg ctcggttttg
     5401 cgcatttggg cgtgctcgat gccatggctc aatgtggcat taattacgat gtgctctgtg
     5461 gcagcagtat gggcggtatt gtggctttga cggttgcaca cttgggcagt atcgaccagg
     5521 cgattcagca agtgcgcgaa caactcggct ctaaccgcaa gatcaaggat ccatcgttgt
     5581 taccccgtgg atcactattc atcggtaaca aaattgcagc agcggcgaaa gccacctttg
     5641 gtgactgcac gtttgctgat cttaagcacc ctgcagcggt agtcgccgcc gatttggtaa
     5701 cacatgagcg tattatcttt gaccgaggtt tggtcgcgcc tgcggtatta gccacctctg
     5761 caattcctgg attttttcca cccgtggcca atcaatcgcg tttgatggtc gatggcagcg
     5821 tggtttcgtt ggtacctgtg gatgttctga gcaaccgccg ttgtggattg cgcattgcca
     5881 tcaatgtcaa caccaaacct tttgccagtg gcaatgatgt catcacggga ttcgatggat
     5941 tactgcaaaa aacccagaaa ccgttggggc tcaaagatgt actcagcgct tcctggggtt
     6001 tgcttggctc ctggcgttct tctagcgaag cattgcgtgc agaaatcgtg atcaacccgc
     6061 agacgccggt ggaatctgga tacaatttca atgagtttga aaaattggtt gattgtggac
     6121 gtgctgctat gcacgaccgt atcgaatcga ttcaagagtc gatcaagctg atgctgcgcg
     6181 cgtgatggtg tatgtgcggc atggtatcgc caatggctat tgctaccgta ttttgttctc
     6241 attgcaatga accctatttt caaaggggtt catactgata aataggccat ggcacttgct
     6301 ggcatctacg gcatcatcat gattctgacg gatttggttg atcatagtgt gcgtagccca
     6361 ctttcacgaa cccgaaaaac cgatctcaag caataaagtc tgcaaacatt gacttagtag
     6421 caatacagtg tagattcaat tctctacagc agatcgttgg caagattggc caactcagaa
     6481 cgttcccctt tttctaggcg tatatgcgca aacaaaggat ggcctttcaa tcgatcgatt
     6541 atatacgtca agccattgga ttgctcatcc aagtacggcg tatcgatttg gtgaatatca
     6601 cccgtgaaga cgattttagt actttctcca gcgcgagtga taatcgtttt gatttcatgc
     6661 ggcgtgaggt tttgcgcttc atcaataatg aacatcacgt tagataaact ccgtccacga
     6721 ataaacgcga gggccgtaat ggccagtttg ccactttctt ccatctcatc gagtatt
//
LOCUS       DAOQLJ010000121         6547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_4951_length_6547_cov_5.720579, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000121 DAOQLJ010000000
VERSION     DAOQLJ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4951_length_6547_cov_5.720579"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            488..1294
                     /locus_tag="PK522_08695"
     CDS             488..1294
                     /locus_tag="PK522_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNC41849.1"
                     /translation="MKHKNSLVALFAIGFVFFMSNGSAAPINFLAAGSNAASIQATVD
                     SFRNTLGNLNANQPAEFVGGRREINWDAVPDAVSDPNAFPGNFFNGSVVGRARGIEFD
                     PTGSTTGFQVSSLTNPAFGAPGQFQAFSPEKLFRTVGGDEFDIRFFSPSDQVTSVFTT
                     GFGMVFTDADLGTLPTLEFFDAQGNLLHSLTGQATGSFGLGFMGTVFDTAVVSRIHVT
                     IGDANGLVMDDFIFGEPTRQQNQIPEPSSLALMFAGAFYLTRKLTRQAFN"
     gene            complement(1370..1549)
                     /locus_tag="PK522_08700"
     CDS             complement(1370..1549)
                     /locus_tag="PK522_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41850.1"
                     /translation="MHNSNLGRVIKRRTLSSFLVIAIQNVADSSTNTGDQLLAQINQP
                     VESIGVDYSCFVAEK"
     gene            1743..3464
                     /locus_tag="PK522_08705"
     CDS             1743..3464
                     /locus_tag="PK522_08705"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNC41851.1"
                     /translation="MTHQNMQIIEASSPVYSEHQKEFYRRLQYWCLFISVKVILLSGL
                     LLFSINAASADEWIYRMQQGDNLWNLTERHLTHLKFVLPLQRLNHIQNPFQIPPGTEI
                     RIPIQWTRQQSSSALVLRVFGDAQLRRQGLAQPLPVVDGMQIFPGDAISTGEDAYVTF
                     EFADQSILRLQDNSELRFERLEILGNSAVISTEVNLQKGRTESQVPKHSPINSRFRII
                     TPSAVSSVRGTDFRVGANNDRATMNEVIGGLVQVSNQHQSINIPSGFGVLAEANSKSL
                     QSVPLLPPPDLSETPSLYERIPLNIPVRPLSGASGYRAQIALDESFTQLITDFDTASL
                     PIRGGELPDGDYWLRIRGRDSLGLEGFEAKLPIRLNARPEPPFIIAPQHDSVVENQSP
                     ELQWAHQPEVAHYIIAITQKDSDFSTPIIYEPEVKGNRYQLNQSLEPGDYVWKIAAVS
                     AVEGIGPYNDPLSFRVPVPGPALESTDVSDQNISFSWAATEPGQYFHFQFARDEDFKQ
                     PIVDTFIHESKITLPRQDSGRHYLRIKIIEADGTHGSFSPAQMIEIPSKYPYWLFLLL
                     PLIGLLL"
     gene            3461..6337
                     /locus_tag="PK522_08710"
     CDS             3461..6337
                     /locus_tag="PK522_08710"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC41852.1"
                     /translation="MKVSFDFWNNYSFRSALLIIVVACLPFTGWLERFDWMIYDKISY
                     WKQFIPDDQIVIIAIDEKSLQTIGRWPWSRRIHAELIDRLRQAGRNVVALDFLMAESD
                     DNDPEGDRLLGLAIARHGAVVLPIAPIVEPDSQKISLVQPLPMFQRNALLGHVDVELD
                     YDGVARRLFLFAGVNSPDWPALGYVLVRKAGGINAVSDSDYLIRSEINIPGTSDYWVR
                     SHEVLIPFAGSPGTYPRISYERILNDDLVLKQLNNKIIIVGMEAIGLHSGFTTPVTSN
                     HRLMSGIEWHANVVNMLHSGNTINPLAKFWIVFISIIWVLIALIGVNFIQRNKVIFLL
                     MTVMALGMLLVWIMFDFFHVWIAPSAGLIGLILVYPLWSWSRINEFMQSLFITKTFSF
                     NALESVGDGVITTDAESRIVYMNSEAERLIGIGIEQLKGSLLYELLDIKVVRRSRSIE
                     IREISTLVKNIPQLQDQYRLKNYDESKRMVRVMHQSLFDEHKVLMGSVVTISDITDTV
                     ELKQQVSHQAHHDSLTMLPNRTLLLAKLDQMVESAQQTTSIFAVFNIVIDEFKKINDA
                     LGHRAGDSLLKLVTQRLQAIIGEQNVIARWSGDEFMVLFASAQDALQLVPNVAQDIID
                     SISHRFELDGQEIFVTASIGISLYPQDGQTGESVLSHAASAMGLAKQHGGNQFSFYLP
                     DKVTDWTKDKLALEKDFRLALKNNELDVFFQPIINVQLRRVVRMEALVRWIHPIRGFL
                     PPGDFVPMAEQIGLINQMGELVLYKACKLARELEKIAGPISISVNVAPRQLLQKGFIQ
                     IVETIINEVGLPAELLVLEITESAIIGDMVLASEILSDLKSLGILIALDDFGTGYSSL
                     SLLRELPIDILKIDKSFIRTIDGNVHDFTIAQTIIGLGKNLGKTVIAEGVETANHMKI
                     LRDHACYLQQGFYFSRPLPFAQLIRYFEKNELAVATLCEYFPTN"
BASE COUNT         1812 a         1199 c         1507 g         2029 t
ORIGIN      
        1 aatgcctgtt tgagtcacac ccagattaaa ctgaaaatag tttagaggca tattcgacta
       61 aatatgacca tattgaaggt ttgaagcgtc attttgctga tcaattagaa aagttgttag
      121 cgactaggaa attttagttg ggtattccgg taagaattcg ctttatcaat gcgcaagaat
      181 ctgtagtaaa gcatctaagt ccatgaataa tcaaaggcat tgcaaagagc tacagaagat
      241 ttttccgtac tgctttaatc aaataattaa cctccggcat cgatggacag gattgatacc
      301 atctgatatt ccaggaacga tagtagtaga ggtggcttgc ctagctgcct tggtagacct
      361 attgcatcgg cttgtataac taaaaaatgc tttacaatta atcacttgtg ggatttctat
      421 atcacacatg aataattatc attaatcttt ctaatatgat tatcaagcat acgataagga
      481 gtagattatg aaacataaaa atagtttagt ggccttgttt gctatcggtt tcgttttttt
      541 catgagtaat ggttctgcag cccccataaa tttccttgca gcggggagta atgctgcaag
      601 tattcaagca accgttgata gttttagaaa tacgttaggg aatttaaatg caaatcaacc
      661 cgcggagttt gttggcggtc gacgagaaat caattgggat gcagtgccag atgcggtttc
      721 agatcctaat gctttcccag gtaatttttt taatggcagt gttgtagggc gagcacgtgg
      781 aattgaattt gatcctacgg gttctacaac tggcttccaa gttagttcac taactaaccc
      841 agcttttggt gcgccaggcc agtttcaggc attttctcct gaaaaactat ttcgaacggt
      901 gggtggcgat gaatttgata tccgattctt ttctccgtca gatcaagtaa catctgtatt
      961 cacaacagga ttcggaatgg tttttaccga tgcagatctt ggtactctac ctacattaga
     1021 attctttgat gctcaaggta atctgttgca ctcactgaca gggcaggcaa ccggaagttt
     1081 tggtcttgga tttatgggaa cagtattcga tactgcagtt gtttcccgca ttcatgtgac
     1141 gattggtgac gcaaatggct tagtcatgga tgattttatt tttggagaac caacacggca
     1201 acaaaatcaa attcctgaac caagctcctt ggcattaatg tttgcaggcg ctttttattt
     1261 aactagaaaa ttaactcgac aagctttcaa ctaagagcgg gtcgatttca gtaatagtac
     1321 cctaaattag gcagcaagtt aactaacttg ctgcccctcc aatgtttagt tatttttctg
     1381 caacgaagca ggaataatca actccaatcg attctactgg ttgattgatt tgcgccaata
     1441 attgatctcc cgtatttgtg ctgctgtcag ccacattctg aatcgcaatg accaagaatg
     1501 acgatagggt acgtctttta attactcgac caagattact attgtgcaca gttattggtt
     1561 aataaatata ctgattaggc tattgtaaca agttgataaa tagaacaata tagtgcttaa
     1621 tcggttagag atgagaaagt cgaagttaga acttatgtaa cgtgttattg ggaaatagct
     1681 aatattccat tgtgttttat ctgcgatgag cagaatcaag atttaatcgg agttatgagt
     1741 taatgacgca tcagaatatg cagattatag aagcttcctc tcctgtttat agcgagcatc
     1801 aaaaagaatt ttaccgacgt ttgcagtatt ggtgcttatt tataagcgtt aaagtaattc
     1861 ttttatcagg gctgttgtta ttttcaatca atgcagcaag cgctgatgag tggatttaca
     1921 gaatgcagca gggcgataac ctttggaatt taacagagcg acatctaact cacttgaaat
     1981 ttgttttacc ccttcaacgc ttgaaccaca ttcagaatcc atttcaaatc ccgcctggaa
     2041 ctgagatacg aatcccaatt caatggaccc gccagcagtc gagttctgct ttagtattgc
     2101 gcgtttttgg tgatgctcag ttgaggcggc aaggcttggc ccagccttta cctgttgtag
     2161 atggtatgca aattttccct ggggatgcaa ttagtactgg agaggacgct tatgtcacgt
     2221 ttgagtttgc agaccaatcc attttgagat tgcaagataa tagcgaatta cgattcgaac
     2281 gattagaaat tctgggtaat agcgctgtca ttagcacaga ggttaatttg caaaaagggc
     2341 ggacagaaag tcaggtaccc aagcattctc caataaacag ccgttttaga atcattacac
     2401 cttctgccgt gagttcagtc aggggaaccg attttcgggt cggagcaaac aatgatcgtg
     2461 cgacgatgaa cgaagtcatt ggtggtttag ttcaggttag taaccagcat caatcaatta
     2521 atatcccgag tggttttggt gtgttggcag aagcgaacag taaatcatta caatctgtcc
     2581 cgttactacc tccacctgac ctaagtgaaa cgccttccct gtacgagcgt attcccttaa
     2641 atattccggt gagaccatta tcaggggcga gcggttatcg agcccagatt gcactcgatg
     2701 agagttttac gcagcttata acagattttg atactgctag tttgcccata aggggtggag
     2761 agcttccgga tggggattac tggcttcgga ttcgaggccg tgattcgctg ggtctggaag
     2821 gttttgaagc aaaattacca atccgtctta atgcacgtcc ggaaccgcca tttataatag
     2881 cgcctcagca tgattcggtc gtggaaaatc agagccccga gttgcagtgg gcgcatcaac
     2941 cggaagtggc gcactatatt attgcgatta cgcaaaagga cagtgatttt tctacaccga
     3001 taatctatga gcccgaggtg aaaggaaatc gttatcagct caatcagtct ttggagccgg
     3061 gtgattatgt gtggaaaatt gcagcggtat ctgcagtaga gggaataggt ccctacaatg
     3121 atccattatc ttttcgtgtt cccgttccag gacccgcgct ggaatcaacc gatgtgagtg
     3181 atcaaaatat cagcttttct tgggcagcga ccgaaccagg gcaatatttt cattttcagt
     3241 ttgcgcggga tgaggatttt aagcagccga ttgtagacac gtttattcat gaatctaaaa
     3301 ttaccttacc gcgccaggat agtgggcgcc attacttacg cattaaaatc attgaagcag
     3361 atggtacgca cggttcattt agccccgctc aaatgattga aattccctca aagtatcctt
     3421 attggttgtt tctccttttg cctttgatcg gcttattgtt atgaaggtga gttttgattt
     3481 ttggaacaat tattcttttc ggtctgcatt attgataatc gttgtagctt gcttaccttt
     3541 tacgggttgg ttagaacgtt ttgactggat gatttatgac aagatttcct attggaagca
     3601 atttatccct gatgatcaga tcgttattat tgcaattgat gaaaagagct tacagaccat
     3661 tggtcgttgg ccgtggtcga gaagaattca tgcagaactg attgatcgac ttcgccaggc
     3721 gggacgtaat gtcgttgcat tggattttct catggcggaa tcagatgata acgatcctga
     3781 gggcgatagg ttgttgggat tggcgattgc tcgacatggt gctgtggtgc ttccgattgc
     3841 acccattgtt gaacctgatt ctcaaaaaat ttcattggtt cagccattgc cgatgtttca
     3901 acgtaatgcg ctattggggc atgtcgatgt ggaactggat tatgatgggg tggcacgccg
     3961 acttttttta tttgcgggcg tgaattcgcc cgattggcct gcacttggct atgtgttagt
     4021 ccggaaagca ggaggcatca atgcagttag tgattctgat tatcttattc gaagtgaaat
     4081 caatattccg ggcacgagtg attattgggt tcgttctcat gaggtactca ttccttttgc
     4141 cggttctcca ggaacctatc ccagaatatc gtatgagcga atcttgaatg atgatttggt
     4201 tcttaagcag ttaaataaca agatcatcat cgttgggatg gaagcgattg ggttgcattc
     4261 cggatttaca acgcctgtga cttcaaatca tcgtttgatg agcggtatcg aatggcatgc
     4321 taatgtggtg aatatgctgc atagcggtaa tacgattaat cccctggcta aattttggat
     4381 tgttttcatt tcgattattt gggtgctgat tgccctaatt ggagtaaatt tcatacaaag
     4441 aaataaagtc atttttttat tgatgacggt catggcgcta ggtatgttac tcgtctggat
     4501 catgtttgat ttttttcatg tttggattgc acctagcgcg gggttaattg gtctaatttt
     4561 ggtttatcca ttatggagtt ggtcgcgtat taatgaattt atgcaatcac tctttattac
     4621 caaaacgttc tcattcaacg cccttgaatc agtaggagat ggcgtgataa cgacagatgc
     4681 tgagagtcgg attgtttata tgaatagtga ggcggaacga ttaatcggta tcggtattga
     4741 gcagcttaaa ggtagtttat tgtatgaatt actcgatatt aaagtggtgc gacgcagtag
     4801 aagtattgaa atacgtgaaa tcagcacgtt agttaaaaat atccctcagt tgcaagacca
     4861 atatcgttta aagaattacg atgaaagtaa gcgtatggtt cgagtaatgc atcaatcatt
     4921 gtttgatgag cataaagttt tgatggggtc agtggtaacg atttcagata ttactgatac
     4981 ggtagaactt aaacagcaag tttcccatca ggcgcaccat gactcattga caatgctgcc
     5041 taatcgaact ttgctattag ctaaacttga ccaaatggtg gagtctgcgc agcaaacgac
     5101 ttcaattttt gctgttttta atattgttat cgatgaattt aagaaaatta acgatgccct
     5161 gggccatcgc gctggtgatt cacttctgaa actggtaacg caacgtttgc aagcaatcat
     5221 tggagagcaa aatgtgattg cacgttggag tggagatgaa tttatggtgt tattcgcttc
     5281 tgcacaagat gcgctgcaat tggttcctaa cgttgctcag gatattattg acagtatcag
     5341 ccaccgattt gagttggatg ggcaagaaat tttcgtaacg gctagtatcg gtatcagttt
     5401 gtaccctcaa gatgggcaaa ccggggaatc agtattaagt cacgctgcaa gtgcgatggg
     5461 gctagcaaaa cagcacggtg gtaatcaatt tagcttttat ttaccggata aagttactga
     5521 ttggactaag gacaagttag cacttgaaaa ggatttccgc cttgcgctta agaacaatga
     5581 gctagatgtt ttttttcaac cgattattaa tgttcaattg aggcgtgttg tccgtatgga
     5641 ggcgcttgtt cggtggatac atccgattcg aggtttttta ccgccagggg attttgtacc
     5701 catggcagag caaatcggct tgattaatca aatgggtgaa ctcgtattat ataaagcttg
     5761 taaattggca cgtgagttgg agaaaatagc cgggccgatt agtatttcag ttaatgtggc
     5821 accccgccaa ttattacaaa agggttttat ccagatcgtt gagacgatta tcaacgaggt
     5881 tggtttacca gctgaactgc tagtgcttga aataactgaa agtgcaatta ttggggatat
     5941 ggtacttgct tctgaaatac tttccgacct aaagtccttg ggcatattaa tcgcgctaga
     6001 tgattttgga actggttatt cttcgcttag tttactgcgt gagctgccaa tcgatattct
     6061 aaaaattgat aaatcattta ttcgaacgat tgatggtaat gtgcatgact ttaccattgc
     6121 acaaacgatt attggtcttg gtaaaaatct tggaaaaacg gtaatcgctg agggagttga
     6181 gacagctaac catatgaaaa ttctgcgaga tcacgcctgc tatctgcagc aaggttttta
     6241 ttttagtcgc ccattgcctt ttgcgcagct aatacggtat tttgaaaaga acgagctagc
     6301 cgttgcgact ttatgcgagt attttccaac taattgattc gcaactaatt tatagcaaaa
     6361 catcactaaa ctaagatgat aaggagcata aaatgatgca attactgaat aggtatcgca
     6421 attttgggtt catttttatg gggattgcac tggcgatttt gccgcttgct gcagtggctg
     6481 aacaaattgg cagcgtatca accaaattca aagtgctagg tcccaatgat aaaatcgtaa
     6541 tcgaagc
//
LOCUS       DAOQLJ010000122         6538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_4960_length_6538_cov_5.828783, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000122 DAOQLJ010000000
VERSION     DAOQLJ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4960_length_6538_cov_5.828783"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="PK522_08715"
     CDS             complement(<1..1109)
                     /locus_tag="PK522_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNC41853.1"
                     /translation="MKIAIIGAGCSGLSAIKQLAAAGLTDITCFEKTDRLGGNWAYTE
                     KPGYSSLYQATRTISSKAMSQFSDFPMSADYPDYPSHEQILAYFQAYARYFGLEKFIR
                     FNLGVTQADKTLDDRWKLTLSDSTEQIYDYLVVANGHLTVPRHPDWKDQFLGTYLHVR
                     DYKTNRGFENKHVLVVGAGNSGCDCAVDIGRVAAQVDLSLRSPQYIIPKYIMGNPTDT
                     FASGFDWLPQWLQDGIQKMILRSQIGRYQDYQLPEPTFPPRQAHPTLNSELLPCMREG
                     KIKPRKAIDRIVDQTVYFSDGSFAQYDIILAATGYQIAFPFFPKGLMDWETSLSIPLY
                     LRMFDPQHANLAFIGLLQPQGCLWKLTEVQAKLLSL"
     gene            1265..1870
                     /locus_tag="PK522_08720"
     CDS             1265..1870
                     /locus_tag="PK522_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNC41854.1"
                     /translation="MQKYFGYLVAVLSLVLTGCGYNTLQSTDEQIKASWSEVVNQYQR
                     RADLIPNLVNTVKGFAEQEKDVLLGVTNARSKVTGIQATPELVNNPEAFTKFQAAQGE
                     LSNALSRLMVVVENYPQLKSDANFRELQAQLEGTENRITVARNRYIKAVQDYNVTVRS
                     FPSNLTAMLFGFQNKPNFTVENEQAISVAPKVDFGTPAKAH"
     gene            1889..2776
                     /locus_tag="PK522_08725"
     CDS             1889..2776
                     /locus_tag="PK522_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HNC41855.1"
                     /translation="MTSRPRMRLTELTLFSTLLLVIQCWFIALVNAEAAIPPLKSRVT
                     DLTHTLSTQESSQLEQQLAQFEASNGSQIALLIVPSTQPETIEQYAIRVVDAWKLGRK
                     GVDDGVLLLVAKNDRALRIEVGYGLEGILPDAVAKRIIEETIVPAFRQDDFFGGLQAG
                     VNQIIRVIQGESLPPPKKVSPGLNVILENSIPILVVFLVLGKVLQAMFGRMFGATATG
                     AIAGSIVWLIFSSLAAAALIAIIVFVISLFEDTGRGIYRGGRGHWPGGGLGGGGGFGG
                     GGFSGGGGGFGGGGASGRW"
     gene            2792..3295
                     /locus_tag="PK522_08730"
     CDS             2792..3295
                     /locus_tag="PK522_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNC41856.1"
                     /translation="MNVFRISCHLMTGQFSVRRAFPTVLLDSIEQSIKSSEHRHAGEI
                     VFAVEAALDLASLLKDKPARERAIDVFSMLRVWDTELNNGVLIYLLMADRDVEIIADR
                     GIHAKVGQVGWERICQEMEAAFRQGQFEQGILTGIEAITQQLQTFFPPIHPSGANELP
                     NRPVVLK"
     gene            complement(3327..3719)
                     /locus_tag="PK522_08735"
     CDS             complement(3327..3719)
                     /locus_tag="PK522_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HNC41857.1"
                     /translation="MNTMIRTLPCPCDNEKNYSECCSAYLDEGKFAPSAETLMRSRYT
                     AYVLQRDDYLMKTWHPETRPINLNLITDQSKWLGLTVKRSEQTAPDQAIVEFIARYKV
                     NGKAQRLHEISRFKRIDKQWYYLDGDIL"
     gene            complement(3731..4231)
                     /locus_tag="PK522_08740"
     CDS             complement(3731..4231)
                     /locus_tag="PK522_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41858.1"
                     /translation="MLKIKSILVLALLGISQSVVSANTHCSHQEVIVFNCNIGKKVVS
                     ICASPNLSAKTGYLQYHFGPIHSPELVFPSKKIAPHDQVTGGTLTFSGGGGAYLRFIR
                     NHHRYVVYTAIGRGWGEKAGVNVEKNGHRKANLICRNQPISELGPDFFERAAIKQDPI
                     GFELPE"
     gene            complement(4265..5059)
                     /locus_tag="PK522_08745"
     CDS             complement(4265..5059)
                     /locus_tag="PK522_08745"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HNC41859.1"
                     /translation="MTRYKEGDSGKDIKRIQTALKDKGFNPGALDGEFGPGTEAAVIA
                     FQKSEGLVADGVIGTQTLKALGLIESDQPESFDLQGVTSINVSKMFPQTPISHIKTHL
                     PFVLDALKDAGLADRQMILMALATIRAETASFEPISEGKSKYNTSPNGHSFDLYDNRK
                     DLGNRGKPDGDRYKGRGFVQLTGRANYTKFGKEISQDLINNPDLANDPGIAAQLLARF
                     LKKGERKIRACLDDHDLAGARKVVNGGKHGLVEFSEAYNIGNKLFA"
     gene            complement(5103..>6538)
                     /locus_tag="PK522_08750"
     CDS             complement(5103..>6538)
                     /locus_tag="PK522_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41860.1"
                     /translation="HYSRSHRLGELYESEIYQSVDDGHTNQIPRIMPELLIKPEGEAD
                     TQTHKPKHYNWLRRAKIPALVINSTSLNSGHNWQFTARSMGEPPSQIDGEIDNNTRYR
                     RMYYEHTPNETLQKYRLGYAVAASACVPGLFEPLTIADLYQDKIVRLVDGGVHDNQGV
                     AGLLDEGCTRILCSDACGQMDDVDQPSDSRPGVVLRASSILQDRVREAEYQDLSNRLD
                     SHALDGLFFVHTKKELESQPLDWINCQDPRTPAHLQDPKTSYGIDRELQKKIAGIRTD
                     LDAFTEVEAYAIMASGYRIAKREFELLQNQYQKEGHTDHWGGYDIDAPGTDWPFRKLE
                     PIMGLKAEANKQSQDLHFQLHVARKIFFKTYDLRLDYRIIVWSLLALLIAGILSFLYQ
                     QWNTPLTDTLHLGSLTWGKLFSLLILMVISALIPMVHLLSPSNKVANIFKFMVAAGGS
                     LLAKFHLAVLDRWFLKRGKLERLLKLK"
BASE COUNT         1733 a         1409 c         1560 g         1836 t
ORIGIN      
        1 aggctgagta atttcgcttg cacttcggtt agtttccaaa gacaaccttg gggctgtaat
       61 aaaccaataa aggctaaatt ggcatgctga ggatcaaaca ttcttaagta aaggggaata
      121 gagagcgacg tttcccaatc cattaaaccc tttggaaaaa aaggaaatgc aatttgatat
      181 ccagtggctg cgaggatgat atcgtattgc gcaaaggatc catcgctgaa ataaacggtt
      241 tgatcgacaa tccggtctat tgctttgcgg ggtttaattt ttccctcgcg catgcacggt
      301 aacaattcag aatttagggt agggtgagct tgcctcggcg gaaaagtcgg ctccggtaat
      361 tgataatctt gataacgccc aatttgtgag cgcagaatca ttttttgaat tccatcctgg
      421 agccattggg gtaaccagtc aaaacctgaa gcaaaggtat cagtgggatt tcccataata
      481 tatttgggga tgatatattg cggagagcgc agactaagat caacttgagc ggctacgcgg
      541 ccgatatcca cggcgcaatc gcaacctgag ttgcctgcac cgactaccag tacgtgtttg
      601 ttttcaaatc cacgattggt cttataatcg cgtacatgga gataggtacc aaggaactga
      661 tctttccagt cggggtgccg cggaacggtc aagtgaccat tggcaacgac cagataatcg
      721 tagatctgct cagtgctatc actcaatgtg agtttccaac gatcgtcgag tgttttgtct
      781 gcctgtgtta cgccaagatt gaatcgaata aatttttcta acccaaaata ccgggcatat
      841 gcttgaaaat aggctaggat ttgttcatga ctggggtaat ctgggtaatc agccgacatg
      901 ggaaaatcac tgaactgcga cattgctttg ctcgaaatgg ttcgagtagc ttgataaagg
      961 ctgctatagc caggtttttc ggtgtaggcc caatttccac ccaaccgatc agttttttcg
     1021 aagcaggtaa tatcggttaa gccggcagca gccagttgtt taatggcgct aagacccgaa
     1081 caaccggcgc caatgatagc gattttcatg ggtttgtgat ttagctgaaa tacaaagggt
     1141 tgcgtaccag tcagtataat gcacgacggc aaccaaactg tagatgatta aataaaacga
     1201 ttgcatttaa gcaacgcttc ctttagtttt aagagcatca atcgaatcgg agaggagtcc
     1261 tggcatgcaa aaatatttcg gttatctcgt agcggtttta tcgcttgttc tgacagggtg
     1321 tggctataac accttgcagt cgaccgacga gcaaatcaag gcaagctggt cagaagtggt
     1381 gaatcagtat caacgcaggg cggatttgat acccaatctc gtcaataccg tgaaaggctt
     1441 tgctgagcag gaaaaagacg tcttgctcgg tgtgaccaat gcccgatcaa aggtgacggg
     1501 tattcaagcg acacctgagc tcgtcaataa tccagaagcg tttaccaaat tccaagcggc
     1561 tcagggggaa ttatccaatg ccctgtcacg cttgatggta gtcgtggaaa actatccgca
     1621 acttaaatct gatgccaatt tcagagaatt gcaagcgcaa ttggagggaa cagagaatcg
     1681 cattaccgtg gcgcggaacc gttatatcaa ggcggttcaa gactataatg tgacagtccg
     1741 atcgtttccc agtaatttga ctgcaatgtt gttcggcttc caaaacaaac caaactttac
     1801 agtggaaaat gaacaagcta tttcagttgc ccccaaagta gattttggca cacctgctaa
     1861 agcgcactaa tgctttgttt cctaatcgat gactagtcgt ccgcgcatga gattgaccga
     1921 attaacccta ttcagcacat tgctgctggt aatacagtgc tggttcattg cgctggtgaa
     1981 tgcggaagct gcaattccgc cactgaaatc aagggtgacg gatttaacgc atacgctatc
     2041 cacgcaggaa tcgagtcaac ttgagcagca attagcccag ttcgaagcga gcaacggcag
     2101 ccagatcgca ctattgatcg taccatcgac gcagcccgaa accatcgaac aatacgccat
     2161 ccgtgtagtt gatgcctgga aattgggaag aaagggcgtg gatgatggcg tactgctact
     2221 cgtggcaaaa aatgatcggg cgttacgcat tgaggttggt tatggattag aaggcatcct
     2281 gcccgatgcc gtggcaaaac gtatcatcga agaaaccatc gtgcccgcat ttagacaaga
     2341 tgattttttt ggtggattac aagcgggtgt gaatcaaatc atccgagtca ttcagggaga
     2401 atcattacca ccccctaaaa aagtatctcc cggtctgaat gttattctgg aaaacagtat
     2461 tcccatcttg gtggtatttt tggtgctagg caaggtactg caagcgatgt ttggtcgtat
     2521 gtttggggcg accgcgaccg gtgcgattgc tggatcgatt gtctggttga ttttttcttc
     2581 attagccgca gcagccctga ttgccatcat cgtatttgtg atcagtttgt ttgaagatac
     2641 tgggcgtggt atttatcgtg gaggacgcgg tcattggccg ggtggtggat taggaggagg
     2701 gggtggtttt ggcggaggag gattcagcgg tggtggtggc ggatttgggg gcggtggtgc
     2761 ttctgggcgc tggtaacaga ggaattatca catgaacgtg ttccgtattt cttgccatct
     2821 catgacaggg cagtttagcg ttcggcgtgc ctttcctaca gttttactcg actctattga
     2881 gcaatcgatc aagtcttcag aacatcgaca cgccggggag attgtatttg ctgttgaagc
     2941 agcattggat cttgcgtcgt tgttgaaaga taaacccgca cgggaacgag ccatcgatgt
     3001 tttttccatg ttgcgagtat gggataccga gctcaacaat ggcgtcctca tttatttact
     3061 catggcggac cgcgatgtag aaatcattgc cgatcgcggt attcatgcta aggtcggtca
     3121 agtaggctgg gagcgcattt gccaagagat ggaagctgct tttcgtcaag gtcagtttga
     3181 gcagggaata ttaacgggaa ttgaggcgat cacgcagcaa ttgcagactt tctttccacc
     3241 aatacatcct agtggggcca acgaattacc gaacaggccc gtcgttctga agtaaaacta
     3301 taaattcata aaaagacggc aatgtcttac aaaatatcgc catctagata gtaccattgt
     3361 ttatcaatgc gtttaaaacg actgatttca tgaaggcgct gcgctttacc attcacttta
     3421 taacgtgcaa taaactcaac gattgcttga tcgggtgcag tttgctcaga gcgttttact
     3481 gtcagcccca gccattttga ttgatcggtt attaaattca agtttattgg gcgtgtttct
     3541 ggatgccacg ttttcatcag ataatcatcg cgctgtaata cataggcagt gtaacgtgaa
     3601 cgcattaatg tctcagcaga aggtgcaaat tttccttcat ccagatatgc actacagcat
     3661 tcagaataat ttttttcatt atcgcaagga caagggaggg tacgtatcat tgtattcatt
     3721 gtaatgccaa ctactccggt agctcaaaac cgattgggtc ttgtttgatt gctgcacgtt
     3781 caaagaaatc agggccaagt tctgagatgg gctgattacg acagattaga tttgctttcc
     3841 gatgaccatt tttttcaacg ttgactccag ctttttctcc ccaaccacgg ccaattgcgg
     3901 tataaacaac ataacgatgg tgattgcgga taaaccgaag ataagcacca ccacctcctg
     3961 aaaaagtcaa agtaccccct gtcacttgat cgtggggtgc aattttttta cttggaaaaa
     4021 caagctcagg ggaatgaatg gggccgaaat gatattgcag atagccagtt tttgcactga
     4081 ggtttgggga agcacaaatt gatacgactt ttttaccaat attgcaattg aaaacaatta
     4141 cttcctggtg actgcagtgt gtattggcag aaacaactga ttgactgatt ccaagtaaag
     4201 ctaaaactag aatcgacttg attttcagca cggcaaaagc acttgtttaa tgtctccgaa
     4261 ttattcaggc aaaaagcttg tttcctatgt tgtaagcctc tgaaaattca actaatccat
     4321 gtttcccgcc attcacgact ttcctcgctc cagctaaatc atggtcatcc aagcatgccc
     4381 taattttacg ttcacccttt ttcagaaagc gggctaacag ttgcgctgca ataccgggat
     4441 cattcgctaa gtcaggatta ttgattagat cttggctgat ttctttgccg aattttgtat
     4501 aattagctcg cccggttaat tgtacaaatc cccgtccttt atagcgatct ccatcaggtt
     4561 tacctcgatt gcctaaatct ttgcggttat cgtataaatc aaatgagtga ccattgggcg
     4621 atgtgttgta tttcgatttg ccctcgctaa tcggttcgaa acttgcagtc tcagcacgaa
     4681 tcgtagcaag agccatgagt atcatttgcc tatccgctaa gcccgcatct tttagggcat
     4741 caagcacaaa aggaaggtgg gttttgatat gactgatggg ggtttgtgga aacattttcg
     4801 atacattaat cgaagtaaca ccttgtagat caaaggattc aggctgatct gattcaatta
     4861 gacccagcgc tttcagggtt tgggtaccaa ttaccccatc cgccactaga ccttcacttt
     4921 tctggaaagc gatgacggca gcctctgtac ctggaccgaa ttcaccatcg agtgcacccg
     4981 gattgaaccc cttatctttt agcgctgttt ggatgcgttt aatatcctta ccagaatcgc
     5041 cttctttgta gcgggtcatg gtttttcccc tctgttgtta ggtcgtacaa tatgtttcat
     5101 cgttatttta atttcaacaa tcgctcaagt ttgccgcgtt tcaagaacca acgatcgagc
     5161 actgccagat gaaattttgc taataaacta ccgccagctg caaccataaa tttgaaaata
     5221 ttagctactt tattgcttgg acttagcaaa tgaaccattg gtatcaatgc actaatgacc
     5281 attaatatca atagactgaa caattttccc cacgtgagcg atcctaaatg gagcgtgtca
     5341 gtcagtggcg tgttccattg ttgataaagg aaactgagta tacctgctat tagcagcgct
     5401 aataatgacc aaacgatgat gcgataatcc agtcgcaaat cgtaggtttt aaagaatatt
     5461 ttgcgtgcaa cgtggagctg aaaatgtaaa tcttgacttt gtttgttagc ttcagctttg
     5521 agccccatga tgggttctag tttacggaaa ggccaatcgg ttcccggtgc gtcgatatcg
     5581 tatccgcccc aatgatcggt atgaccttct ttttggtatt gattttgtaa cagctcaaat
     5641 tcccgtttag caatgcgata accgcttgcc atgatggcat aagcttcaac ttcggtaaat
     5701 gcatcaagat cggtgcggat ccctgcaatc ttcttttgta gttcccgatc gatgccatac
     5761 gatgttttag ggtcttgcag atgagcaggc gtacgaggat cttggcaatt gatccaatcc
     5821 aatggttggg attcaagctc ttttttggtg tgcacaaaaa ataagccatc gagcgcatga
     5881 ctatccaagc ggttgctaag atcttggtat tccgcttccc gtactcgatc ctgtaagatt
     5941 gagctagcgc ggagtacgac accaggacgt gaatcggaag gctgatcgac gtcatccatt
     6001 tggccacagg catcgctgca cagaatacgg gtacaacctt catcgagcaa gcctgcaact
     6061 ccttggttat catgtacgcc accatcgact aggcgcacga ttttatcttg atacagatca
     6121 gcgattgtta atggctcaaa taaaccggga acgcaagctg aggctgccac ggcgtagccc
     6181 aagcgatatt tttgtagggt ttcgttaggg gtatgttcat agtacatacg gcgatagcgt
     6241 gtattgttat caatttcacc atcgatttgg ctgggtggct cgcccatgga acgcgcggta
     6301 aactgccaat tatgtccgga gttcaatgaa gtactattga tcacgagcgc tgggattttg
     6361 gctcggcgta gccaattata gtgctttggt ttatgcgtct gagtatccgc ttccccttcg
     6421 ggtttgataa gcaattcagg catgatgcgc ggtatctggt tcgtatgccc atcgtcaacc
     6481 gattgataaa tttctgactc atataattcc ccgaggcggt ggctgcgaga ataatgtt
//
LOCUS       DAOQLJ010000123         6496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_5008_length_6496_cov_5.822854, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000123 DAOQLJ010000000
VERSION     DAOQLJ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5008_length_6496_cov_5.822854"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PK522_08755"
     CDS             complement(<1..335)
                     /locus_tag="PK522_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNC41861.1"
                     /translation="MVFLFSACTSYPLLTNQGEKIAHDYLIGPGDTLNIIVWRNPEIS
                     MSVPVRPDGKITTPLVEDLPASGKTSTQLARDIEETLSKYLQQPVVTVIVTGFVGPFT
                     EQIRVIGEAT"
     gene            complement(841..1917)
                     /locus_tag="PK522_08760"
     CDS             complement(841..1917)
                     /locus_tag="PK522_08760"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNC41862.1"
                     /translation="MQKIKVLVIDDSALICKILAEIINNQPDMEVAGVAHDPIVAREL
                     IRELNPDVLTLDIEMPKMDGLDFLERLMRLRPMPVLMISTLTEANSEPALLALELGAV
                     DFISKTKFDMATGLESFSDEVVSKIRMSVYAKIKKSTANQSEQSVKQNLSYGTNPTNW
                     GNKLIIVGASTGGTEAIREFLMELPPDVPGILIAQHMPESFTKPFANRLNTQCRITVV
                     EAQGGERVLPGHAYVAPGHSHLLLKRSGGYYMTELSQSEPVNRFRPSVDVLFRSAATH
                     AGKDALGVIMTGMGKDGAAGMLEMHRAGAYTFAQDEESCVIFGMPREAIAMGGVDEIV
                     PLKNMARQVLRRLAYSDKPNQLRA"
     gene            complement(1977..2570)
                     /gene="cheD"
                     /locus_tag="PK522_08765"
     CDS             complement(1977..2570)
                     /gene="cheD"
                     /locus_tag="PK522_08765"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF010013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HNC41863.1"
                     /translation="MAMDDGITYQYAKYSYFNCKLNREVIKLLPGEFYVSDNDVVMET
                     VLGSCIAACVYDSNQGIGGMNHFLLPAPPTQDREKDRSSARYGEYAMDILISEVIRMG
                     AIRSNLEVKLFGGGNVQHSMTAMNIGGKNADFALDYVKRKEIHLAGYDLVDIYPRKII
                     FYPKDGLVMVKKLMIEPASIINDEKNYESILKNQIDK"
     gene            complement(2634..5963)
                     /locus_tag="PK522_08770"
     CDS             complement(2634..5963)
                     /locus_tag="PK522_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNC41864.1"
                     /translation="MLVSKTTPKGVVSYVNEPLIEMSGFTEQELIGQAYNIICHPDMP
                     AEVFDDYWKTIKRGKPWDGLVKNRAKDGSYYWVYESVSPIIEGGQISGYLMLRNKPTH
                     DQIRAAEELYRTMREGSCKYKLREGQLFKKGFRGDLSHRLKFITIKHRIVAMVLSSLI
                     GMAIVSGLGLFGASQSNDRLRTVNEDRLVPVKDLSDIRDSMQEMRLQSMRLSYLTDQK
                     NVKTYFDQIMQLDKIFNEKWAKFKATSLTAEETELANHFSKQAEIYMQARNQFLNMIG
                     NSDFVAARNYLEQETDPKLSQAFLALQNLQALQETIAQSEYSDAGIALKSISSAITIA
                     VLFIACLLIYMGINLIRAVIKPISDISQVIKRVAEGDFSTQLEVRNFEEIGLLTEEIK
                     MMQNRSGFEFNEAKRVASESLRIKNALDQSNSSIMIADSNGQIIYLNQAAEALFKTNE
                     VGFAKELPEFKAGNLLGASFNLFNQKMNLMSITQSQKVDTVLGGRIFSLVFVPVINEQ
                     QQRLGTAIEWIDRTQEIAVEKEVSGVVSAAVDGDFKKRLTLEGKSGFFKHLSEAINKL
                     METTSTGLADVTRVLDGLAHGDLSQKIEGDYKGTMGQLKDSANQTVTQLNEIVSQIKM
                     MVAGAAAGDFSQRGDVTQYQNEFKEMIKGLNQLMQICESGLKDMVRVLNALAKGDVSQ
                     KIEGDYKGTFDQLKSSSNQTIAQLNEIVSQIKIMVAGAAAGDFSQRGDVTHYQNEFKE
                     MIEGLNQLMQICELGLKDMVRILNALAKGDLTQKMTNEYEGIFGQLRDDSEQTVTQLT
                     VIVNKIRESVESINTASRDIADGNNDLSQRTEEQAANLQETASSIEELTSTVKQNAEN
                     AKHANELAASASGVAIKGGEVVGRVVHTMSSINDSSKKIADIISVIDGIAFQTNILAL
                     NAAVEAARAGEQGRGFAVVATEVRALAQRSAAAAKEIKELISDSVNKVEDGTQLVDQA
                     GKTMDEIVTSVKHVTEIMAQIASASQEQSEGIEQVNDSITKMDEITQQNASLVEEAAA
                     ASESMREQAEQLAQAVRVFKLAQGSAPNQSVQKVERRGPDRAKNVERIHSSSAADKAK
                     KGRYVLPDPAKSGTDDDWEAF"
     gene            complement(6088..>6496)
                     /locus_tag="PK522_08775"
     CDS             complement(6088..>6496)
                     /locus_tag="PK522_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNC41865.1"
                     /translation="TMDEIVSSVKRVTDIMAQIALASQEQSLGIEQVNDSITKMDEIT
                     QQNASLVEEAADASESMREQAEQLAQAVKVFKLAQGVISNKNAQMTDRRGPNRAKNVE
                     RIHLSSAVDNAKTGRYILSDPAKSGTEDDWETF"
BASE COUNT         1774 a         1588 c         1059 g         2075 t
ORIGIN      
        1 gttgcttcac cgattactct aatttgctca gtaaatggcc ctacaaatcc agttacaatc
       61 actgtgacga ctggctgctg caaatatttt gaaagtgttt cttctatatc tctggctaat
      121 tgagttgaag tcttcccact tgccggcaaa tcttcaacta aaggggttgt tattttccca
      181 tcagggcgaa ccggaacaga cattgatatt tcaggatttc tccaaacgat gatattcaaa
      241 gtgtccccag gcccgattaa ataatcatgt gcaatcttct ctccctgatt cgttaggaga
      301 ggatagctag tacatgcgct aaataaaaat accaaacata caattaaaac tgctatggat
      361 cttcttaaaa attgatcgtt atttgggata tccactttat gcctcattaa aaaaatcgct
      421 aaaagaattt agaacattcc atatcacttg gatgttaaac caaaaaaaaa aattcaaaca
      481 ccataaaatg gatgaatttg atggcatttc tgaagataag atgctgatga cgcaacttat
      541 ttactattaa agaaatttgc aaacagtatc aggacttgcc agttacatta atactatatt
      601 gtggaactaa ttcgttgcaa gctacccgaa atttgggaat aactcgagtc aatatgaatt
      661 taaaaatcga cttccttagt ttttctggaa attaggctat atctatagtc tcattttaac
      721 cagattcatg cggttaatta tcatatatca acaaaactta gttttttttg taacattatt
      781 acaacgcttt attacaatac gttatcaaat catccgcagc aataattcag gccactcaac
      841 ctaagcacga agctgattgg gcttatcaga ataagcgagt cttctcagaa cttgcctagc
      901 catgttttta agcggaacaa tttcatcaac gcctcccatt gcaatcgctt ctcttggcat
      961 accgaatatt acgcaactct cttcatcttg tgcaaaggta taagcgcccg ctcgatgcat
     1021 ctctagcata cctgcggcac catccttacc catacccgtc atgataaccc ctaatgcatc
     1081 tttacctgcg tgagtggcgg cggatctaaa taaaacatcg actgaagggc gaaaacgatt
     1141 caccggctca gattgactaa gctcagtcat ataatatcca ccgcttcgtt tgagtagaag
     1201 gtgagagtgg cctggcgcta cataagcatg cccaggtaat acccgctcac ctccttgggc
     1261 ttcgacaaca gtaattctgc attgcgtatt caaacgattt gcaaatggct ttgtaaagga
     1321 ttcgggcata tgttgtgcaa ttaaaatgcc tggaacatca ggcggtaatt ccattaagaa
     1381 ttctctaata gcctcagttc ctccagtaga cgcgcccact ataatgagct tatttcccca
     1441 atttgttggg ttggttccgt aagaaagatt ttgtttcacg gattgttcag attgattagc
     1501 agtagatttc ttaatttttg catatacact catacgaatt ttgctgacca cttcatcact
     1561 aaagctctcc aaacccgtag ccatatcaaa tttagttttt gaaataaaat caacagctcc
     1621 aagctcaagc gctaataaag ccggttcaga attagcttct gtcaatgttg agatcatcaa
     1681 taccggcatg ggccgcaaac gcataagcct ttcaagaaaa tctaaaccat ccattttagg
     1741 catttcaata tcgagcgtga gtacatctgg attaagttct cgaatgagct cccgtgcaac
     1801 aatcggatca tgagctacgc ccgccacttc catatcaggt tgattattaa taatttctgc
     1861 caaaatttta caaattagtg ctgaatcatc aataactaat accttgattt tttgcatcta
     1921 ctcacctctt tatcaatatt tgcttgattt ttaatttcat gattcagcct tatcaatcat
     1981 ttgtctatct ggtttttcaa aattgactca tagttctttt catcattgat aattgaagct
     2041 ggttcaatca ttagtttctt aaccattaca agtccatcct ttggatagaa aataattttg
     2101 cgtggataaa tatcgactaa atcgtaacct gctaagtgaa tttctttccg tttcacataa
     2161 tctaatgcaa aatcagcgtt tttccccccg atgttcattg cggtcatact gtgctgcaca
     2221 ttgccgccgc caaacaactt aacctccagg ttgctgcgta tcgcccccat tctgatcact
     2281 tcactaataa gaatatccat agcgtactct ccataacgtg cagaagatcg atctttctcc
     2341 cgatcttggg taggtggcgc aggcaacaag aagtgattca tgccaccgat accctggttt
     2401 gaatcataaa cacaggcagc aatacaagaa cccaatacag tttccattac tacatcgtta
     2461 tcactcacat aaaactcacc aggtaatagc tttatgactt cacgattaag tttgcaatta
     2521 aagtaactat attttgcata ttgataagtt attccgtcat ccatggccat tcgaattatt
     2581 tctatgaata ttcataaccg ttagtaacaa gatgacactg atctcgcaca aaactagaat
     2641 gcttcccaat cgtcatcggt accagattta gcaggatcag gtaatacata ccgccctttc
     2701 ttagctttat cagctgcgct cgatgaatga attcgctcta cattcttcgc acgatccggt
     2761 ccacgtcgct ctaccttttg tacgctttga ttaggcgctg atccttgagc tagcttgaat
     2821 actcttactg cctgtgcaag ttgttctgct tgttctcgca tcgattccga tgccgctgcc
     2881 gcttcctcaa ctagcgatgc attctgttga gtgatctcgt ccatcttcgt gatcgaatcg
     2941 tttacttgct caataccctc actttgctcc tgcgatgccg atgcaatctg cgccataatt
     3001 tctgtcacgt gcttcactga agtcacgatc tcatccattg tcttacctgc ctgatctacc
     3061 agctgagttc catcttcaac cttattaacc gagtcactaa tcaattcttt gatttcctta
     3121 gctgcagccg ctgagcgttg cgccagcgct cgcacctccg ttgccactac cgcgaaacca
     3181 cgcccctgtt cgcctgctct ggctgcctca acagcagcat tcaatgccaa aatattggtc
     3241 tgaaaggcaa tgccgtcaat cacgctgata atgtcagcaa tcttcttcga gctatcattg
     3301 attgagctca tggtatgcac aactcgcccg accacttcac cacccttaat cgccacgcca
     3361 gaggctgatg ctgccaactc atttgcatgt tttgcattct ccgcattctg ttttaccgtt
     3421 gaggtcaatt cctctatgct cgatgctgtc tcttgcagat tcgctgcttg ctcttccgta
     3481 cgttgtgata gatcattgtt cccatctgct atgtccctgg atgccgtatt gatggattca
     3541 actgattctc tgatcttatt cacaatcacc gtcaattgcg ttaccgtctg ttctgaatca
     3601 tctcgcaatt gtccaaatat cccttcgtac tcgttcgtca tcttttgcgt caaatcaccc
     3661 ttcgctaacg cattcaatat ccttaccata tcttttaaac ccaattcaca aatctgcatc
     3721 agctgattca atccttcgat catttccttg aattcattct gataatgggt aacatctcct
     3781 cgctgactaa aatctcccgc agccgcaccc gctaccatta tcttgatctg cgatacaatt
     3841 tcattgagct gcgctatagt ttggtttgag ctactcttta attgatcaaa cgtgcctttg
     3901 taatcccctt caatcttctg tgacacatct cccttcgcta atgcgtttag cactctcacc
     3961 atatctttta aacccgattc acaaatttgc atcagctgat tcaatccttt gatcatttcc
     4021 ttgaattcgt tctgatactg agtaacgtct cctcgctgac taaaatctcc tgcagccgca
     4081 cccgctacca tcatcttgat ctgcgataca atttcgttta actgcgttac tgtctgattt
     4141 gcgctatctt tcaattggcc cattgttcct ttgtaatcac cttcgatctt ttgagataaa
     4201 tcgccatgtg ccaacccgtc taaaacacgt gtaacatctg caagtcctgt agaagtcgtt
     4261 tccatcaact tattgattgc ctcagataaa tgtttgaaga aacctgactt tccttcaagg
     4321 gtcaaacgtt tcttaaaatc accatcgacc gcggcagata caacaccgga tacctctttt
     4381 tccaccgcaa tttcctgagt acgatcgatc cactctatcg cagtacctaa acgttgctgt
     4441 tgctcattga tcactggaac aaaaactaac gaaaaaattc tcccgccaag gacagtatca
     4501 accttctggc tttgggtaat actcattaaa ttcattttct gattaaacag attaaagctt
     4561 gcgccaagca aattcccggc tttaaattca ggtagttctt tagcaaatcc aacttcattt
     4621 gttttaaata gcgcctcagc agcctgattc aaataaataa tttgaccatt ggaatcggca
     4681 atcatgatcg aggagttcga ttggtctagt gcattcttga tgcgtaagct ttctgaagcc
     4741 actcgttttg cttcattaaa ctcaaatcca gatcggttct gcatcatctt tatctcttca
     4801 gtcaaaagcc caatttcctc gaagttacgc acttcaagct gagtactaaa atcaccttca
     4861 gccactcgtt taattacttg actaatgtca ctaataggtt ttatcactgc gcgtattagg
     4921 ttaatcccca tataaatgag tagacaagca ataaacagaa ccgcaattgt gatagcacta
     4981 gaaattgatt ttagggctat tcccgcatca ctatattctg actgcgcgat cgtttcctgt
     5041 aacgcttgca aattctgtaa cgctaaaaaa gcttgactaa gctttggatc agtttcttgt
     5101 tccaaataat ttcttgctgc tacaaaatca ctattcccaa tcatattcaa aaattggtta
     5161 cgggcctgca tataaatttc agcttgttta gaaaaatgat ttgctaattc agtttcttca
     5221 gcagttaaac tagtcgcttt aaattttgcc catttttcat taaatatctt atcgagctgc
     5281 ataatttgat caaaatacgt tttcacgttc ttttgatcag tcaaataaga caatctcatt
     5341 gattgcaatc tcatttcttg catgctatcg cgtatatcgc ttagatcttt aactggaact
     5401 aaacgatctt cattaactgt acgcaaccta tcattacttt ggctggctcc aaacaatcct
     5461 aaccctgaaa caatagccat tccaatgaga gagcttaata ccattgcaac aatgcggtgt
     5521 ttgatcgtga taaattttaa tcgatgactc agatcgcccc ggaatccctt tttaaacaat
     5581 tgcccttcac gaagtttata tttgcaactc ccttcacgca tcgtacgata tagctcttcc
     5641 gccgcccgaa tttggtcgtg cgtaggtttg tttcttaaca tcaaataacc tgatatttgg
     5701 ccaccttcaa tgatcggtga cacactctcg taaacccaat aataagaacc atctttagcg
     5761 cgatttttaa ctaatccatc ccaaggcttt cctctcttaa tcgttttcca ataatcatca
     5821 aacacttctg ccggcatatc ggggtgacaa atgatgttat atgcttgccc tatcaattcc
     5881 tgctcagtaa aaccactcat ttcaatcaaa ggctcgttaa catagctaac cacacctttc
     5941 ggggttgttt tggaaaccag catctcgcca tccctcattt ccttttcaac attgcttact
     6001 ggcatattga atcgcacgtc acacctacct ttttcaaaga gttaaattaa tcaatcgact
     6061 attgaaaatc tctaaactta tgtattacta aaatgtttcc caatcgtctt cagtgcctga
     6121 tttagcagga tcagataata tgtatcgtcc tgtcttagca ttatcgaccg cactagacaa
     6181 atgaatccgc tctacattct ttgcacgatt tggcccacgt cgatcagtca tttgtgcgtt
     6241 tttattagat atcacccctt gcgctaattt gaataccttt accgcctgtg ccagttgttc
     6301 tgcctgctca cgcattgatt ccgatgcatc ggctgcttcc tctactagcg atgcattctg
     6361 ctgggtgatc tcatccatct tcgtgattga gtcatttact tgttcaatac ctaaactttg
     6421 ctcctgtgat gcgagagcga tttgcgccat aatgtctgtc acacgtttca ctgacgatac
     6481 aatctcatcc attgtt
//
LOCUS       DAOQLJ010000124         6474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_5034_length_6474_cov_6.642156, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000124 DAOQLJ010000000
VERSION     DAOQLJ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5034_length_6474_cov_6.642156"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PK522_08780"
     CDS             complement(<1..643)
                     /locus_tag="PK522_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HNC41866.1"
                     /translation="MQGPEYFTYLSAQHLESLNLSAEAIIQSIEQLLTTARQSHAWNA
                     PKSVIMPDQRYLMATLSAADDPPFLAVKALVLNPCNHARGLADINALVTLLDSDTGVP
                     LAIIDGNWVTAVRTAGLSAVAAKRLAKPDASVIAFIGCGVQARSHLKLFAQLFPLKEL
                     KAFGRGASNRNALCQLGEALGLRSIASESAQAAICDADLIISTVTFSTQLVPFL"
     gene            complement(668..1108)
                     /gene="trxC"
                     /locus_tag="PK522_08785"
     CDS             complement(668..1108)
                     /gene="trxC"
                     /locus_tag="PK522_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HNC41867.1"
                     /translation="MQESIHIVCPHCDAINRIAEHRLSDNPNCGKCHGALFEEQPLEL
                     SAARFHKHIGQNDIPVLVDFWAPWCGPCKMMAPHFAQAAAQLEPHIRLAKVNTEIEQM
                     LGAQYSIRSIPTLVLFSHGQEVARQSGAMGATDIVRWALSHHSG"
     gene            1336..2799
                     /gene="guaB"
                     /locus_tag="PK522_08790"
     CDS             1336..2799
                     /gene="guaB"
                     /locus_tag="PK522_08790"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNC41868.1"
                     /translation="MRLIQKALTFDDVLLVPAHSVVLPRDVDLTTHLTRTIKLKLPLV
                     SAAMDTVTEARLAIAIAQEGGIGIIHKNMPINVQAAHVAKVKRFESGIVSEPITIPPN
                     MTVRGVLELIQQHKISGLPVVEDSKVVGIVTNRDLRFETNLDQPIKNIMTPKKRLVTV
                     KEGASREEALTLLHKHRLERVLIINDQYELKGLITVKDITKTTEHPNASKDSQERLRV
                     GAAVGVGEGSKERAEALIEAGVDVLIVDTAHGHSQSVLDQILWVKKQFPSAQVIGGNV
                     ATATAAKALVDHGADAVKVGIGPGSICTTRIVAGVGVPQITAIENVANALQGTGVPAI
                     ADGGIRYSGDIAKAIAAGANTVMLGGLFAGTEESPGEIELYQGRSYKSYRGMGSLSAM
                     QQGSSDRYFQEHKQTEANKLVPEGVEGRVPYKGSIVAVIHQLVGGVRSSMGYLGCKTI
                     DEIHAKAEFIEITSAGIRESHVHDVQITKEAPNYHVE"
     gene            2814..4373
                     /gene="guaA"
                     /locus_tag="PK522_08795"
     CDS             2814..4373
                     /gene="guaA"
                     /locus_tag="PK522_08795"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNC41869.1"
                     /translation="MHHKILILDFGSQYTQLIARRIRETNVYCELHPYDVSADFIKAF
                     SPIGIILSGGPASVFIEDTPRAPQIVFELGVPVLGICYGMQVMAAQLGGAVESAEKRE
                     FGYAELAIQDGPLFTGLQDRIDSSGKTLLDVWMSHGDSVTRLPENFKIIADNAATPIA
                     AMADEKRKFYGLQFHPEVTHTLQGKAILEHFAHDICAAGYDWNMPDYIEESVSRIRAK
                     VGNEKVILGLSGGVDSAVAAALIHRAIGDQLTCVFVDNGLLRLNEANQVMDVFSKHLY
                     AKVVHVDASQRFLSKLQGITDPEQKRKIIGKEFVEIFQHEAEKLVDAKWLAQGTIYPD
                     VIESADSKTKKAHTIKSHHNVGGLPETLHLKLLEPLRELFKDEVRELGIALGLPHDMV
                     YRHPFPGPGMGVRILGEVKYEFAELLRRADAIFIEELRISGWYERTSQAFAVFLPVKS
                     VGVMGDGRTYEYVIALRAVHTQDFMTAHWAELPYTLLGKISNRIINEVRGINRVVYDI
                     SGKPPATIEWE"
     gene            4499..5725
                     /locus_tag="PK522_08800"
     CDS             4499..5725
                     /locus_tag="PK522_08800"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HNC41870.1"
                     /translation="MKRIEIAVNQLKLGMFVSALDRPWLETPYLIEGVLIKSVEDITE
                     LARHCNYVYVDIEKSNPQSISNYAGQGTKNSSQADAATPFHGKAVYVDRCTAEKELPA
                     AQKAYDTTITLIDEIFECIERNTQLDIFAAQTAVESLRESVLRNADALLLLSRLKHSK
                     QALYDNALKVSVLLLAFGRHLGLPREELSELGLGGLLMDIGKLHLPPELLKKTTALTA
                     KEQEIMQKHVENGEAILARTPNIPEAVAKIVSQHHEREDGDGYPRKLTANQLHNFARM
                     AAIVDSYEDLTTARSGIKGLKPFQIIVELKNSARSGLNSVLVEQFSHCIGMFPVGSLV
                     ELNSREVAIILTHNRSQRFHPKVMVILDAKKQPYEKPFELDLKSTKPAPSGVPYEIVR
                     DLPLGAYNIDASQYYL"
     gene            5762..>6474
                     /locus_tag="PK522_08805"
     CDS             5762..>6474
                     /locus_tag="PK522_08805"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC41871.1"
                     /translation="MAAITYQDFLNKASEVLQDPAHLNHKKALLLIDFEKLPALESAV
                     GFFPIDNILQQAFDLINHALHPSDIVGITGRHQICCLLTQLRSESHALLAAHKVLRIF
                     QQRFPLGERLIVLFPRIGIAFSDLCKNELGRLLGNAGSALQQARFEHEPIKVYEKKEE
                     DTILLGIDLWSDLGNAIEEGELTLYYQPQIDLATEKIISTEALIRWNHPQRGLIQPDK
                     FINAYELFSVFFILYSKIN"
BASE COUNT         1874 a         1462 c         1394 g         1744 t
ORIGIN      
        1 cgagaaaagg gactaattga gtagaaaaag taactgtcga gatgatgaga tccgcatcac
       61 aaattgctgc ctgtgcactt tcactcgcaa tcgatcttaa accaagtgct tcgcctaatt
      121 gacatagtgc attgcgattg ctagcgcccc gaccaaatgc cttcaactct tttagtggaa
      181 atagctgcgc aaacagcttt aaatgactcc gggcttgaac accacatcca ataaacgcga
      241 taaccgatgc atcaggtttt gctagccgct tggcagcaac cgcactcagt ccagcagtac
      301 gaacagcggt aacccaattc ccatcgataa tggcaagagg cacccctgtg tcgctatcca
      361 ataatgttac taacgcattg atatcagcca atcctcgcgc atgattgcac ggattgagaa
      421 ccaacgcttt aaccgctaga aagggcggat catctgctgc agataaggtt gccatcaaat
      481 agcgctgatc cggcatgatg acactcttag gtgcattcca ggcatgagat tgccgcgctg
      541 tggtaagtaa ctgttcgata ctttgaataa ttgcttctgc tgaaagattc aggctttcaa
      601 ggtgctgggc agataaataa gtaaaatatt caggtccctg catcacacaa attcgctagc
      661 aagaccttta cccagaatgg tggctaagtg cccaccttac aatatcggtc gcccccattg
      721 ctcctgactg acgcgctact tcttggccgt ggctaaaaag gacgagtgtc ggaatactac
      781 ggatactata ttgtgcgccc agcatctgtt cgatttccgt attgacttta gccaaacgaa
      841 tgtgcggctc caattgcgct gctgcttgcg caaagtgagg cgccatcatt ttgcagggcc
      901 cacaccaagg tgcccaaaaa tcaaccaaca ccggaatatc attctgaccg atatgtttat
      961 ggaatcgtgc cgcagataat tcaaggggct gttcttcaaa taaagcgccg tgacatttgc
     1021 cacagttagg gttatcactc aagcgatgct cagcaatacg attaattgca tcacagtgtg
     1081 gacaaacaat atggattgat tcctgcatga ttttgttgcg actaaataat aagtaatcga
     1141 aaggatttta gccattatgg catgatttag tcgaattctt acttaacaac agatttgtaa
     1201 caaataaaca ctactgaaat cagaaccaga aaaagtaggc attgtaaaaa caaaattcac
     1261 tgccaaccct cattttcctt cccgataaga aattgatttc gttataataa cgctttgtaa
     1321 cgaatcgtta ttatcatgcg actgattcaa aaagctctga ccttcgatga tgtattatta
     1381 gttcccgcac actcggtcgt attgccacgt gacgttgatt taacgacgca tttaactcga
     1441 acgatcaaac tcaagctgcc gcttgtttcg gctgccatgg ataccgtaac ggaagcccgg
     1501 ctcgccattg ccattgcgca agaaggtggc atcggtatca ttcataagaa tatgcccatt
     1561 aatgtgcagg cagcacatgt tgccaaagta aagcgttttg agagtggtat tgtctccgaa
     1621 ccgattacga ttccaccgaa catgactgtg cgtggtgtgc tcgaattgat ccagcagcat
     1681 aaaatctcag gacttcctgt cgtagaggat tccaaagtag tgggtatcgt tacaaaccgc
     1741 gatcttcgct tcgaaacaaa tctcgaccag cccatcaaaa acatcatgac acccaagaag
     1801 cgtcttgtta ccgtcaagga aggcgcttca agagaagaag cgcttacgct tttacataaa
     1861 catcgcttgg aaagagtgtt aattatcaat gatcagtatg aattgaaggg tcttatcacc
     1921 gtaaaagaca ttactaaaac aaccgaacat cccaatgcca gcaaagatag tcaagaacgg
     1981 ttgcgagtcg gtgctgcagt tggggtggga gaaggtagta aagaacgcgc tgaagcgctc
     2041 atcgaagccg gtgtcgatgt actgattgtc gatacggccc acggccattc tcagagtgtg
     2101 ctcgatcaga tcctttgggt caaaaaacaa tttccatctg cccaagtcat cggcggtaat
     2161 gttgcgacag caacagcagc caaagcacta gtcgatcatg gtgccgatgc agtcaaagtc
     2221 ggtattgggc ctgggtcgat ttgtactact cgcattgttg caggcgtggg tgtcccacaa
     2281 atcacagcca ttgaaaatgt tgctaatgcc cttcagggta ctggcgtacc agccattgca
     2341 gatggtggca ttcgctactc aggcgatatt gccaaagcca ttgctgcagg ggcaaacact
     2401 gttatgcttg gtggtctttt tgctggcact gaagaatcgc ctggcgaaat tgaactatat
     2461 caaggccggt cttacaaaag ctatcgaggc atgggatcac tttctgctat gcaacaagga
     2521 tcaagcgatc gctatttcca agaacacaaa caaactgagg caaacaagct agttccagaa
     2581 ggcgttgagg gaagagtccc ctataaagga tcgattgtgg cagtcattca tcaattggta
     2641 ggaggtgttc gctcaagcat gggttacctc ggttgtaaaa ccattgatga aattcatgct
     2701 aaagctgagt ttatcgaaat cacttcagca ggaattcgtg aatcacatgt gcacgatgta
     2761 caaatcacca aagaagctcc taattatcac gtcgaataac aagcaagtca tctatgcacc
     2821 ataaaatcct catcttggat tttgggtctc agtacaccca attgattgcc cgtcgcatcc
     2881 gggaaactaa tgtgtattgc gaattacatc cttatgatgt tagcgctgat tttattaaag
     2941 cgttctctcc tattggcatt attctatccg gcgggcctgc atccgttttt atcgaagata
     3001 ctccccgtgc ccctcaaatc gtgttcgaac tcggcgttcc cgtactcggc atctgttatg
     3061 gaatgcaagt catggcggca caacttggcg gtgctgtcga atcggctgaa aaacgtgagt
     3121 ttggctatgc agaactcgct attcaagatg gcccactttt taccgggtta caagatcgaa
     3181 ttgattcaag cggaaaaaca ctactggatg tttggatgag tcatggcgac agcgtcacgc
     3241 gcttacccga aaattttaaa atcattgccg ataatgctgc cacacctatc gcagcaatgg
     3301 cagatgaaaa gagaaaattt tatgggctac agtttcatcc tgaagtaacc cataccctgc
     3361 aaggaaaagc tattctcgaa cacttcgcgc atgatatctg cgcagctggc tatgattgga
     3421 atatgccaga ctacattgag gaatcagtca gtcgcatccg cgccaaagtc ggtaacgaga
     3481 aagttattct cggtttatcg ggtggtgttg actctgctgt cgccgctgcg ctcatccacc
     3541 gggcgattgg tgatcaactc acctgcgttt ttgttgataa tgggttgctc cgcctaaatg
     3601 aagcaaatca agtaatggat gttttttcca aacaccttta cgccaaagta gtgcatgtcg
     3661 atgcgagcca gcgatttctc tcaaaacttc aaggtattac cgacccagaa cagaaacgta
     3721 aaatcattgg gaaggaattt gttgagattt ttcaacacga agctgaaaag ctggttgacg
     3781 caaaatggct agcacaaggc acgatctacc ctgatgtgat tgaatctgct gatagcaaaa
     3841 caaaaaaagc acacaccatt aagtcccatc ataatgtggg tggattacct gaaacacttc
     3901 atttaaaact gcttgagcct ttacgtgaat tatttaaaga cgaagtacgc gagttaggta
     3961 tcgcgttagg acttcctcat gatatggttt atcgccaccc ttttccgggg cctggaatgg
     4021 gggtcagaat tctcggcgag gtcaaatacg aatttgcaga attgctaagg cgcgctgatg
     4081 caatctttat cgaagagctt agaatttcgg gatggtatga aagaacatct caagcttttg
     4141 ctgttttttt accggttaag tctgtcggtg tcatgggcga tggccgtacc tatgaatatg
     4201 taattgcact tcgtgccgtg catacacaag acttcatgac agctcattgg gctgaacttc
     4261 cctatacgtt gctcggtaaa atctctaacc gaatcatcaa cgaagttcgt ggtattaatc
     4321 gcgtcgttta tgatatctcc ggtaaaccgc ctgcgacaat tgagtgggaa tgaaatcata
     4381 gctacttaat ttcattcact ctgttctttt ccgaatcaag cgcttaaaca gcataaacct
     4441 caaaaaagta actcttttcc aagattctgc taagttaaaa ccctctaaaa ttgccctgat
     4501 gaagcggata gagatagcag taaatcaatt gaaattaggt atgtttgtta gtgccttaga
     4561 taggccctgg cttgagacgc cttatctaat tgagggcgtt ttgatcaaat ctgtcgaaga
     4621 catcactgag ttagctcgtc actgcaacta cgtctacgtt gatatcgaaa aaagcaatcc
     4681 ccaatccatt tcaaattatg cgggtcaagg tacaaagaat agcagtcaag cggatgctgc
     4741 aacccccttt catggtaaag cggtttatgt tgatcgatgc actgctgaaa aagaactacc
     4801 cgcagcacaa aaagcgtatg acactacaat tacactcatc gatgaaattt ttgaatgtat
     4861 cgaacgcaat actcagcttg atatttttgc agctcaaact gctgtagaat cgctgcgaga
     4921 aagtgtttta cgaaatgccg acgcattact cctactttcg cgccttaagc attcaaaaca
     4981 agcgttgtat gataacgccc ttaaagtctc tgttttatta ctcgcttttg gccgccacct
     5041 tggcctccca agagaagaat tatctgagct tggcctcggt ggtttgctca tggatattgg
     5101 taaacttcac cttcctccag aacttctcaa aaaaactacc gcacttaccg ccaaagaaca
     5161 agaaatcatg caaaagcatg ttgaaaacgg cgaagctatc ctggctagaa cgcctaatat
     5221 tcctgaagca gtcgcaaaaa tagtgtcgca gcaccacgaa cgtgaagatg gcgatggtta
     5281 tccaagaaag ctaactgcca atcaacttca taattttgca cgcatggctg cgattgtaga
     5341 tagttatgag gatctcacga ctgctcggtc aggtattaaa gggttaaaac ctttccagat
     5401 catcgttgag cttaaaaata gcgcacgcag cgggcttaat tctgtactcg tagaacaatt
     5461 ctcccattgc atcggaatgt ttcctgtcgg ctcactcgtt gagctcaatt ctcgcgaagt
     5521 tgcgatcatt ttgacgcata atcgctctca gcgatttcat cccaaagtaa tggttatact
     5581 agatgctaag aaacagcctt atgaaaaacc ttttgagctc gacttaaaat caactaagcc
     5641 tgcaccaagc ggggttccct atgagatcgt aagagattta ccattaggcg cctataatat
     5701 tgatgctagc caatattatc tatagtaaat gggttgataa atacacttat ttattttccc
     5761 catggctgcc atcacttacc aagattttct gaataaagca tctgaagtac tacaggatcc
     5821 agctcatctc aatcataaaa aagctttgct actgattgac ttcgaaaaat tacctgcgct
     5881 cgaaagtgca gtcggttttt ttccgattga taatattctg caacaagcgt ttgatctcat
     5941 caatcatgcg ctccatccca gcgatattgt tggaatcact ggtcgccatc aaatttgctg
     6001 tttattgact cagctgcgct ctgagagtca tgcattgtta gccgcacata aagtgctacg
     6061 aatttttcag caacgtttcc cattaggtga acggcttatt gtgctatttc cacggatagg
     6121 catcgcattc agcgatttgt gtaaaaatga attaggccga cttcttggta atgcaggatc
     6181 tgcacttcag caagccagat ttgaacatga acctattaag gtttatgaga aaaaggaaga
     6241 agatacgatt cttcttggta ttgatctttg gtcagacctt ggaaatgcca tcgaagaagg
     6301 agagcttact ttatactatc aaccgcagat tgatttagct actgaaaaaa tcatatcaac
     6361 agaagcgctc atccgctgga atcatcctca gagagggtta atacaacctg acaaatttat
     6421 aaatgcctat gaactattct cagtgttttt catcttatat agcaagatca atag
//
LOCUS       DAOQLJ010000125         6406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_5111_length_6406_cov_6.262636, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000125 DAOQLJ010000000
VERSION     DAOQLJ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5111_length_6406_cov_6.262636"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PK522_08810"
     CDS             <1..623
                     /locus_tag="PK522_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNC41872.1"
                     /translation="KLVKDSGVKTKKALFAEIALGKQFPVVVAKRLTAPYEALSEAFS
                     TSNNPISILGTEGMTIQFAKCCYPIPGDAIAGLIKKDHGLVIHTQDCTAISKYLKNSE
                     NLLNVSWGEEIPNTFQSAIIMTVVNQRGVLARVASEIAKVGSNIDDVKLENDSDFTNM
                     RFILQVRNRKHLAQIMRKLRHIHEIVKLGRVKAGIEHKYHKGSIKL"
     gene            638..1294
                     /locus_tag="PK522_08815"
     CDS             638..1294
                     /locus_tag="PK522_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41873.1"
                     /translation="MENSSILDFTSPGSIPLESSEIVKQVNLEIRISIQTLENILVTD
                     PMETTCWKLLGGLYLGANLTNKFNKLTQQYQNFFGKPLFPEFEEKNRAEERLLFRMPS
                     NIVPELLPNTTEVEQACNSRKKVTIDFSDVKESSAEGLSTLAAFFLSLAKVSNKPEII
                     DINHFILNLEKKAIASNKVNSVWDVLFAYKRLCDDVKGFDNLALKFAIQYSISPPSWI
                     "
     gene            complement(1492..3660)
                     /locus_tag="PK522_08820"
     CDS             complement(1492..3660)
                     /locus_tag="PK522_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41874.1"
                     /translation="MSVRAASSAELIQSGVTAYQTIGTLRRETPINGDAIAAAYTGDL
                     QALVQEIDEANVLELHNDIQGAINDIKNGKAPKLAAQVVDKTLQRVFYQIIWNRISAI
                     RSEFQSGALSTLVSMLDEAEAAFNAIKGTVARENRVLTADRQSIVAGDNPGLDTEVLD
                     QFARVRVALNKTNAEEDFIVVQIARYAIRMSLARAYYIGVLREVAAVIENRNSDVEET
                     LEVLKEGEIFYRIIESQVSNGNPAGNLIIKNQLVGHPSKVVADEIVSELGKGFIGRVL
                     AETSGQERAITEGNRPQAVAEAAGAKYFARVLFPDLELRLGSTVRSNLENELENLLAA
                     SNELNATNSKVARDAIAGILNQYESALNRSKYTVTKPTALIENAVVDFQEIGVLRNQS
                     PADADAIAQKYSGDLKGLTQIVDQIYGLTIDQDVSAAINRVKNGDDTALALQVIDKSL
                     QRMFAIVVYNRVVLTVDQFANLSTDELLIEWERAYSAYLAIARTADREDKILTADKTT
                     IMSGRNPDLDYQILTAFVQGKEVLSKANDDDRASIALAQENIIIPLVRSFLIGVLREV
                     EGIISDRDGNVDEAREKQVEGEYFYRVVEGFISQDNLVGSNLIKTQLTGSLASVEADA
                     IVRQINKGILGQLKRNISQIEVNFASDKSKALLARERLFLLAGIFLSDLELRLGALQR
                     VRLENAIRNLKEAILTDDSSQAIATRAVMTEVIANYESKL"
     gene            complement(4008..6395)
                     /locus_tag="PK522_08825"
     CDS             complement(4008..6395)
                     /locus_tag="PK522_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HNC41875.1"
                     /translation="MTRTIQKQLSKSGIVFFITITTFFSSAGIVHSSALNESAVVNLN
                     IPAASLNEALNQFSKQTGIKIAFDPEQFQGKTAKALIGEFTLKEAINQLISGNKLTAV
                     PVGSGYTLLEIADSSVSNPVVTTLPSIQITATKTASRYLATSSITATKTNTLLRDVPQ
                     SISVITEGIIKDQAIRSLSDAVRYIPGIGVSQGEGNRDALIFRGNRSTGDFFVDGIRD
                     DVQYLRDLYNIERVEVLKGANGMIFGRGGSGGVINRVTKQAGWDPIREFSFQGGSFNL
                     KRMTMDVNQAINEHVAFRVNGLFEDAGSFRNGVESQRLGISPTVTIKPTDRTKIVVNM
                     ERFHDERVADRGIPSFQGRPADVKSSQFFGDPRRSNSNIEVLSFNSLIEHRFESGVML
                     RNRTNYSEFDKFYENVFANGPVFGSFVPLSAYNNATDRQNIFNQTDLLYTLETGPITH
                     TLLAGVEVGQQITNNRRETGFFNNDLSRGQSIRVPFGNSITNAPITFVNAEGDAFNRS
                     KVDITSFYFQDQIELLPQLQAIAGVRYDLFEVNFQQKNGNQDRLNTTDGLLSPRFGLI
                     YKPIEPVSIYVSFSQAFVPRAGEQLTSLTVTTDTLKPEKFTNLEAGFKWDIRPDLSFT
                     GAAFRIDRTNVITIDPTNAARSFLTKGQRTEGVELSLSGQLLPNWNVMGGYAYQIGEV
                     TSDQAGAPKGNSVAELPRNTFSVWSRYDLTPKFGAAIGVIYRSSMFASTSNTVIIPDF
                     TRVDAALFAQLTTRVRAQLNVENLFDAKYFASVHNDNNITPGSPVAIRASLIANF"
BASE COUNT         1986 a         1361 c         1090 g         1969 t
ORIGIN      
        1 ataagcttgt aaaagatagt ggtgtaaaaa ccaaaaaagc actttttgct gaaattgcac
       61 taggaaagca atttcctgtt gttgttgcaa aacgattaac tgctccttat gaagcgctgt
      121 ccgaagcctt tagtactagc aataatccta tatcgatact aggaactgaa ggcatgacca
      181 ttcaatttgc caagtgctgt tatccaattc ctggtgatgc cattgctgga ttgataaaaa
      241 aagatcatgg actagtcatt catacccaag attgcacagc aatttcaaaa tatctaaaga
      301 attcagaaaa ccttttgaat gtgagttggg gcgaggaaat tccaaataca ttccaatcag
      361 ctattattat gacagtggta aatcaacgcg gtgttcttgc aagagtcgct tctgagattg
      421 ctaaagttgg gtctaatatt gacgatgtca aactagaaaa tgatagtgat ttcactaata
      481 tgcgttttat tttacaagtc agaaatagga agcatctcgc tcaaatcatg agaaagctaa
      541 ggcatattca tgaaattgta aaacttggac gagtgaaagc tggtattgag cacaaatatc
      601 ataaaggtag tattaaactt taacttaagt tatcacaatg gaaaattctt ctattcttga
      661 ttttacatcc cctggctcga ttcctttaga gtcctcagag atagttaagc aagttaatct
      721 ggaaattagg atttcaattc aaacactcga aaatattcta gtcactgatc ctatggaaac
      781 aacgtgctgg aaattacttg gagggcttta tctaggagca aacctaacta ataaattcaa
      841 caagcttaca caacaatacc aaaatttctt tggaaaacca ttatttcctg aatttgaaga
      901 aaaaaaccga gccgaagaac gattgttgtt tagaatgcct tctaatattg ttccagaatt
      961 attaccaaac actacagaag tagaacaggc ctgtaactct cgcaaaaaag taacaattga
     1021 tttttctgat gttaaagaat caagtgcaga aggattaagt acactagcag ctttctttct
     1081 atcactagcc aaagttagta ataaacccga aataatcgac ataaatcatt tcatattaaa
     1141 tctagagaaa aaagcgatag ccagcaataa agtaaatagc gtatgggatg tacttttcgc
     1201 ttataaacgt ctgtgtgatg atgtaaaagg gtttgataat ttagctctta aatttgcaat
     1261 tcagtatagt atttctccac cttcttggat ttagcttcaa caatccttag ttatcacgat
     1321 aaaatatttc tgctgaactt gccatcaata tcagtagatt tatgtttttc tcaaaacgcc
     1381 aactaaattt ctgttaagcc actatcgaag ctctcatgta gctgacagtg gctttgtttt
     1441 gatatttatt gattgatcca gttacccagc attctaatat ttatgagaaa gctacaattt
     1501 actctcataa tttgcaataa cttcggtcat cactgcacga gtagcaattg cttgagacga
     1561 atcgtcagta aggatggctt ctttaagatt ccgaattgca ttttctaacc tcactcgttg
     1621 taatgcacct aagcgtaact ctagatccga tagaaatatc cctgcaagta aaaaaagcct
     1681 ctctctggct agcaaagcct tactcttatc acttgcaaaa ttaacctcga tttgactgat
     1741 atttctttta agttggccca gaattccttt atttatttgt ctaacaattg catcagcttc
     1801 aacagaggcc aaactaccag ttagttgtgt cttgatgaga ttgctgccaa ctaagttatc
     1861 ttgtgaaata aatccttcca caacgcggta aaaatattcc ccttcgactt gtttttctct
     1921 agcttcatca acattaccat cacgatcaga aataattcct tctacttctc ttagcacacc
     1981 gatcaagaag cttcttacta agggaataat gatattttct tgtgccaaag caattgaggc
     2041 cctatcatcg tcattagctt tgcttaagac ttctttacct tgaacaaacg cagtaagaat
     2101 ttgataatct agatctgggt tcctgccact catgattgtt gtcttatccg ctgtcagtat
     2161 tttatcttcc cgatctgccg ttctagcaat agccaaataa gcgctataag ctctttccca
     2221 ttcgatgagt agctcatcgg tcgaaagatt ggcaaattga tcaactgtca acactacacg
     2281 attgtaaacc acgatcgcaa acattctctg tagtgattta tcaattactt gcaatgctag
     2341 agcagtatca tcgccatttt ttactcgatt gatcgctgct gaaacatcct gatcgatcgt
     2401 taatccataa atttgatcaa cgatttgtgt caaacctttt aaatcaccag aatatttttg
     2461 cgcaatggca tcggcatctg ctgggctttg gtttcttaaa accccaattt cctgaaaatc
     2521 cactactgcg ttttctatga gcgcagtggg ttttgttact gtatatttcg aacggttaag
     2581 cgcactctca tactgattca gaatccctgc aatagcatcg cgtgctactt ttgagtttgt
     2641 tgcatttagc tcgttactcg ctgctagcaa attttcgagt tcattttcta aattacttcg
     2701 cactgtcgat cccaacctca attccaaatc tggaaataag actctagcaa aatatttagc
     2761 gccagctgct tctgctacgg cctgtggacg attgccttca gtaatggctc tttcttgccc
     2821 gcttgtttct gcaagcactc gaccaataaa tcctttacct aattcactca caatctcatc
     2881 cgcaaccact tttgatggat gacctaccaa ttggttcttg atgattaaat tacctgctgg
     2941 atttccattt gatacctgag actctatgat gcgataaaag atttctcctt cttttagtac
     3001 ttcaagggtc tcttctacat cggaatttct attttcgatc acagcagcca cttcacgtag
     3061 aaccccgata tagtatgccc gcgccaaaga catacgaatc gcgtaacgag caatttgcac
     3121 cacaatgaaa tcttcttcag cattagtttt attcagcgct acccttactc gtgcaaattg
     3181 atcgagaacc tcagtatcta atccaggatt atctcccgct actattgatt gtctatcagc
     3241 agttagcact cgattctctc tggctacagt tcccttgatt gcattaaatg ctgcttctgc
     3301 ctcatccaac atactaacta aggtagataa tgcgcctgac tgaaattcgc tacgtattgc
     3361 tgaaattcga ttccaaataa tttgataaaa aacacgctgc aaggttttat ctacaacctg
     3421 tgcagccaac tttggcgctt tcccattttt aatatcgtta attgcacctt gaatatcatt
     3481 atgtagctct aaaacattcg cttcatcaat ttcttgtacc agcgcttgca aatcacctgt
     3541 ataagctgca gcaatggcat cgccattaat tggtgtctct ctgcgtaacg tcccaatcgt
     3601 ttgataagct gtcacgcccg attgaattag ttcagcactt gaagctgctc ttacactcac
     3661 actacttaac gctaaaccca atgacaataa acttatcaat gatgaagaaa ctattaaatt
     3721 gaataactgc tttcttaaaa gcattacaaa atcccccttg ataattaata ttgaatacga
     3781 aggttaatga taatgattat tattaataaa atcaaatatt gtaaaattat ctttagagag
     3841 attctctaag aaatctgcga tatagctcca agagaagtaa ctagaccgcc agtaccactc
     3901 tacccgagcc ctattaattc aacaagacca gtagcaaatt ttattgcttc aatagcttat
     3961 gtactagagg gagaaaaaca aaacttggag agattaaaag catggcttta gaaatttgca
     4021 attaatgagg cacgaattgc aactggagat ccaggagtaa tattattgtc gttatgcact
     4081 gaagcaaaat atttagcatc aaaaagattc tcaacattca attgagctcg gacccgcgtt
     4141 gttaattgtg caaataatgc ggcgtcaaca cgtgtaaaat ctggaataat aactgtattt
     4201 gatgttgatg caaacatact actacgatag ataacaccga tggctgcgcc gaattttggt
     4261 gttaaatcat atcgactcca aacagaaaat gtattacgag ggagctcggc gactgaattt
     4321 cctttgggag ctccagcttg atcactcgtt acttcaccaa tttggtatgc ataccctccc
     4381 atcacattcc aattcggtag caattgccca cttaaactta gctcaacccc ttctgttcgc
     4441 tgtccttttg ttagaaagga cctagcagca ttagttgggt caatcgtaat aacatttgtt
     4501 ctgtcaattc taaatgcagc acctgtaaac gataaatcag gtcggatatc ccacttaaaa
     4561 cccgcttcaa gattcgtaaa tttttcaggt ttcagcgtat ccgttgtaac agttaacgaa
     4621 gttaactgtt ccccagcacg aggaacaaaa gcttgactaa agctcacata aattgagact
     4681 ggttcaattg gtttataaat caaaccaaac cgaggggata gcaaaccatc ggtagtattc
     4741 aagcgatctt gatttccatt cttttgctga aaattaactt caaacaaatc gtaacgaacc
     4801 cctgcaatgg cttgcagctg gggcaataac tcaatctgat cttgaaaata aaaggaagtg
     4861 atatcgacct tactgcgatt aaaagcatct ccttcagcat tcacaaatgt gatcggcgca
     4921 ttggtaattg aattaccaaa tggtactcga atactttgac cccgtgataa atcattatta
     4981 aaaaaaccag tttcccttct gttgttagta atttgctgac caacctcgac ccctgctaac
     5041 aacgtatgag taataggtcc tgtttcaagg gtatacaaca aatctgtctg attaaaaata
     5101 ttttgacgat ctgttgcatt attataagca ctcaaaggta caaaacttcc aaaaactgga
     5161 ccattagcaa aaacattctc atagaacttg tcaaattctg aatagtttgt tcggttacgc
     5221 agcatgactc cagattcaaa acgatgctcg attagcgagt taaatgacaa aacctcgata
     5281 ttagaattac ttcttcttgg atcaccaaaa aattgagaac tcttaacatc agctggtctt
     5341 ccctggaaag aaggtatacc acgatcagca acacgctcat catgaaaccg ttccatatta
     5401 acaacaattt tagtgcgatc cgttggttta attgttacag tgggcgaaat tccaaggcgc
     5461 tgtgactcaa cgccatttcg aaagctaccc gcatcttcaa ataaaccatt taccctaaat
     5521 gccacatgtt cgttaatggc ctgattaaca tccattgtca ttcttttcaa attaaaagat
     5581 ccaccttgga acgaaaattc acgaatagga tcccacccag cttgcttggt aacgcgatta
     5641 ataaccccgc cagaaccacc gcgtccgaat atcattccgt tagcaccttt taatacttca
     5701 acacgctcaa tattatataa atcacggaga tactgaacat catcacgaat accatcgaca
     5761 aaaaaatctc ctgttgatcg gttaccccta aatattagcg catcacgatt accctcaccc
     5821 tgcgagaccc cgattcccgg aatatatcgc actgcatcac tcaaactccg gatagcttga
     5881 tcctttatta ttccttccgt aataacagaa attgattgcg gaacatctcg aagaagtgta
     5941 ttggttttgg ttgctgtaat gctactagtg gccaaataac gacttgctgt tttggtcgcc
     6001 gttatctgta tcgaaggtaa cgttgtaact actgggtttg atacggagga atcggctatt
     6061 tctagtagcg tataccctga accaactggc accgctgtca gtttatttcc actgattaat
     6121 tgatttatcg cttcttttaa agtgaactcg ccgatcaatg ctttggctgt ttttccctga
     6181 aattgctctg gatcaaaagc aatcttgata ccagtctgct ttgaaaattg attaagtgct
     6241 tcgtttaatg aagctgcagg aatatttagg ttaacaactg ctgattcatt tagcgctgac
     6301 gaatgaacga tcccagcgct tgaaaaaaag gtagtaatgg taataaaaaa aacaattccc
     6361 gacttgctga gttgtttttg aatcgttcga gttatagagg taaatt
//
LOCUS       DAOQLJ010000126         6360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_5181_length_6360_cov_6.580333, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000126 DAOQLJ010000000
VERSION     DAOQLJ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5181_length_6360_cov_6.580333"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /gene="sucA"
                     /locus_tag="PK522_08830"
     CDS             <1..1238
                     /gene="sucA"
                     /locus_tag="PK522_08830"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965018.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNC41876.1"
                     /translation="VNWAPFLNSGKWNQPVVTAVPIDDLIAVAERLTDIPTGFKLNPR
                     IEKILTDRREMGKGRLPLDWGMAENLAYATLLKDNFPIRISGQDSGRGTFFHRHAVLH
                     DQNREQEQWENGTYIPLRHITPTQPDFTVIDSILSEEAVLGFEYGYASAQPNELVIWE
                     AQFGDFANGAQVVIDQFITSGEAKWGRLCGLVMLLPHGYEGQGPEHSSARLERYLQLC
                     AEYNIQVCIPSTPAQIFHLIRRQMIRPMRKPLIVMMPKSMLRNKESVSLLEDLAMGHF
                     QPVIPETENLSPDQVRRIILCSGKIYYELLAYRRKKAITDMVLIRLEQLYPFPHEDFQ
                     QEIDRYGQAKEIIWCQEEPGNQGAWHRIQHYIIRHKRPDQKLGYALRPSSASPSVGYL
                     ALDRFRQNELIEAAFRDNL"
     gene            1252..2493
                     /gene="odhB"
                     /locus_tag="PK522_08835"
     CDS             1252..2493
                     /gene="odhB"
                     /locus_tag="PK522_08835"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNC41877.1"
                     /translation="MLIEIKVPQLSESVAEATLISWHKKQGEKVVRGENLIDIETDKV
                     VLELPATQDGILIEILKNDGATVKSGEIIAKIETEMTSTASVSSSLTINQGTVKPKPE
                     TKNQIAKILMPAAQKMAEENQLKQNEVNSIVGTGREGRVTKEDIANHIKSKPQTAEVT
                     STPASILSEVPNMEQLASNTERLEQRVPMTRLRMRIAERLVQSQSTAAILTTFNEVNM
                     QAVIDLRTRYKDKFEKEHGVKLGFMSFFVKAVVAALKKFPIINASVDGNDIIYHPYYD
                     IGIAVASPRGLVVPILRNADQLTQVEIEKQIADFVTRAQNGKLTIDELSGGTFSITNG
                     GVFGSMLSTPIINPPQSAILGIHATKERPVVENGQIVIRPMNYLALSYDHRIIDGKEA
                     VLSLVTIKEALEYPMSPLFES"
     gene            complement(2516..6022)
                     /gene="dnaE"
                     /locus_tag="PK522_08840"
     CDS             complement(2516..6022)
                     /gene="dnaE"
                     /locus_tag="PK522_08840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNC41878.1"
                     /translation="MPIDPAFIHLRLHSEYSITDSIIRIDEVLARAAQDHMPALALTD
                     LGNLFGLVKFYQSAEKIGIKPIIGCDAWISNEADRNNPFRVLLLCQTHSGYLLLCRLL
                     SRAYRENLFRGRAELKKAWLHSSAEGTEDLIAISGAHFGEVGQLIIQNNLQEAQVVSK
                     EWSELFPNRFYLEIQRNGRVDEERFIRNSLILAAQLNIPIVATHAIQFLDAKDYKAHE
                     ARVCIAEGYVLGDRRRPRAFTDQQYFKTQSEMQALFADIPEALANSVEIAKRCNLQLE
                     LGINRLPLFPVPDTTSIEAYLSNQALSGLKQRLQDLPENEFNHKLTTYQARLNFEVNT
                     IIQMGFAGYFLIVADFINWAKQNNIPVGPGRGSGAGSLVAYSLGITDLDPLRYDLLFE
                     RFLNPERISMPDFDIDFCQDRRESVIEYVKNRYGMESVSQIVTFGTMAAKAVIRDVGR
                     VLDLPFNFVDQLAKLIPFELGMTLKKARAIEPQFESRAREEEDVRNLLELAESLEGIT
                     RNVGMHAGGVLIAPGKITDFCPIYCAEASESVVSQFDKDDVEKIGLVKFDFLGLKTLT
                     ILDQAISDINQQQLHDIKYQTEPESTGGISLNQIALEDPETYALLRKGNAVGIFQFES
                     RGMKDLLQKAKPDRFEDLIALVALYRPGPMDLIPDFIERKHGIQRVEYLDPRLEPILG
                     PTYGVMIYQEQVMQIAQVIGGYSLGGADLLRRAMGKKKVEEMAQQRDIFVEGAIKNGL
                     TEQNAIKLFDLMEKFAGYGFNKSHAAAYALIAFQTAYLKAHYPAAFLAACLSAEMNDT
                     DKINDFYEDCKVNGINVLPPDINFSMYRFVPTDAKTIRYGLGAVKGTGENAITAIVQA
                     RERSGLFTDLFDFCKRVDRRLVNRRSIESLIRVGAFDGIQSNRAMLFASVGSAMEFAE
                     QSSLSTQQVNLFSETEVNAQLPTLLEHVAWSEKEKLQNEKLGLGFYFSGHPFSFYLKE
                     LNQFITTRLDKLRSQREPQLIAGIIHAIRTQMTKRGKMAIVTLDDGRARVDIVIYNEI
                     LGEHSSWLKEDQLLVAKVIIGNSMNDPNELRITAEKLYDLTNARSQSAKKIQFTINQS
                     IIDCKLVVPKLKELIAANLPVAKGNHGQVQPTRCPVTILYQNQQATCVIELGDAWHVL
                     LSDFFIESVSQLFKPENVQILY"
     gene            6082..>6360
                     /locus_tag="PK522_08845"
     CDS             6082..>6360
                     /locus_tag="PK522_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNC41879.1"
                     /translation="MQTLPELLLPAGSLEKMRTAYAFGADAIYAGQPRYSLRARNNEF
                     SLEQLRTGIIEAHQLNKKFFVASNLMPHNRKVKTYMADMEPVIDLKPDA"
BASE COUNT         1922 a         1425 c         1223 g         1790 t
ORIGIN      
        1 cggttaattg ggcgcctttc cttaattcag gcaaatggaa tcaaccagtg gtaaccgctg
       61 taccaattga tgatttaata gccgtagctg agagactcac agacattccc actggtttca
      121 aattaaaccc acggattgaa aaaattttga ctgatcgacg cgagatgggg aaagggagac
      181 taccgcttga ttggggaatg gctgaaaatt tagcctatgc aacacttttg aaagataact
      241 ttccaattcg gatttctggt caggacagtg gccgtggcac cttctttcat cggcatgccg
      301 tattacacga tcaaaatcga gaacaagagc agtgggaaaa tggtacttac attccactaa
      361 gacatattac gcccacgcaa cctgatttca ccgtgatcga ttctatactt tcggaagaag
      421 ccgttttagg gtttgagtac ggctatgcct ctgcccaacc caatgaatta gtcatttggg
      481 aggctcaatt tggtgacttt gcaaatggtg ctcaggtagt cattgatcaa ttcatcactt
      541 cgggagaagc taaatgggga cgattatgtg gtttggtaat gctgcttccc catggctatg
      601 agggtcaagg ccctgagcat tcctcagcac gattggaacg ttatctgcaa ctctgcgctg
      661 agtataatat tcaagtttgc attccgtcta cgccagctca aatatttcat ttgattcgca
      721 gacaaatgat acgccccatg cgcaagccat taatcgtcat gatgcccaag agtatgctaa
      781 gaaataaaga atccgtttct ttactcgagg atcttgctat gggtcatttt caacccgtta
      841 ttccagaaac ggaaaatctg tcacctgatc aggttagacg gatcatattg tgtagtggaa
      901 aaatttatta tgagctactc gcatatcgaa gaaaaaaagc aatcacggat atggtgctca
      961 ttcgtcttga acaactttat ccttttcctc atgaagattt tcaacaagaa attgatcgct
     1021 atggacaagc aaaggaaatc atttggtgtc aagaagaacc tggaaatcaa ggcgcttggc
     1081 atcgaattca acattatatt attcgacata aacgtcctga tcaaaaactt ggctacgcgt
     1141 tacgaccttc ctcagcttca ccatccgtag gatatttggc attggatcga ttcaggcaaa
     1201 atgaactcat cgaagcagct ttcagagaca acctttaata aggaaaataa tatgcttatt
     1261 gaaatcaaag ttcctcaatt atccgaatca gtcgctgaag ccactttgat ttcttggcat
     1321 aagaagcaag gtgaaaaagt agttcgtggt gagaatttaa tcgatattga aaccgataaa
     1381 gttgtacttg agctacccgc aacgcaggat ggcatcttaa ttgaaatact aaaaaatgat
     1441 ggagcaaccg tcaaaagtgg ggaaatcata gccaagattg aaacagagat gacttccaca
     1501 gcctcagtta gctcaagttt gacgataaat caaggcaccg tgaaacctaa accagaaact
     1561 aaaaatcaaa tagccaaaat actcatgcca gcagcccaaa aaatggcgga ggaaaatcag
     1621 ttaaagcaga atgaagtcaa ctcaatcgta ggaaccggcc gagaaggtcg cgtcaccaaa
     1681 gaagatatcg cgaatcacat taaaagcaaa ccgcagaccg ctgaggttac ttccacccct
     1741 gccagcatac tttctgaagt acctaatatg gagcaattag cgagtaacac tgaaagactt
     1801 gagcaacgtg tcccaatgac ccgtctcaga atgcgcattg cggaacgact tgtgcaatct
     1861 caatcgacag cagcaattct gaccactttt aatgaagtca atatgcaagc cgtgattgat
     1921 ctacgcacac gatataagga taagtttgag aaagaacatg gggtcaaatt aggtttcatg
     1981 tcgttctttg ttaaagcagt tgtcgctgca ctaaagaaat tcccgatcat caatgcttca
     2041 gtcgatggaa atgacatcat ctatcatccc tattacgata tcggaattgc tgttgccagc
     2101 ccacggggcc tggttgtgcc aattcttaga aatgcagatc aactaacaca agtagaaatt
     2161 gaaaagcaaa ttgccgattt tgttactcgc gctcaaaacg gtaaactcac cattgatgaa
     2221 ttatccggcg gaacattttc cattactaat ggtggtgttt ttggatcaat gctgtctacc
     2281 ccgatcatta atcctcccca aagcgctatt ttaggcattc atgctaccaa agaacgtccc
     2341 gttgttgaaa acggtcaaat tgtaattaga ccgatgaatt atcttgcgct ttcctacgat
     2401 caccgcatta ttgatggtaa agaagccgtt ctctcgttag ttacaatcaa agaagcatta
     2461 gagtatccga tgagtccttt attcgaatct taaacttaga ttcatcgaat cttgctcagt
     2521 atagaatctg gacattttcg ggtttaaata actgagaaac cgattcaata aaaaaatcac
     2581 ttaataaaac gtgccatgca tcacccagct caatcacaca cgttgcctgc tgattttgat
     2641 aaagaattgt aacggggcat cgtgtgggtt gtacttgccc atgattgcct ttagcaacgg
     2701 gtaaattcgc agcaatcaat tcctttaact taggcacaac caatttacaa tcaatgattg
     2761 actgattaat cgtaaattga atcttcttcg cggattgaga acgtgcattg gtaaggtcat
     2821 ataatttctc cgccgtgatt cgcagttcat tgggatcatt catactgtta ccgatgatta
     2881 ccttcgcaac taataattga tcttccttta accaactact atgttcgcct aagatttcgt
     2941 tataaatcac gatatcgacc cgtgcccggc cgtcatcgag tgtaacgatt gccattttcc
     3001 ctcgcttggt catctgcgtt cggatcgcat ggattattcc ggcaatcaat tgtggctcac
     3061 gctgtgatcg aagtttatct aaacgcgtcg tgataaattg attcaattct tttagataaa
     3121 acgaaaaggg atgaccacta aaataaaacc caagacctag tttttcattt tgtaactttt
     3181 ctttttcaga ccatgccaca tgctcaagta aggtaggtag ctgagcatta acttcagttt
     3241 cgctgaatag attgacttgc tgagttgata aactcgattg ttcagcaaat tccattgcgc
     3301 tgccaactga cgcaaataac atagcacgat tggactggat accatcgaaa gcaccgactc
     3361 gaattaagga ttcgatgcta cgccgattca caagccggcg gtctacgcgc ttacaaaaat
     3421 caaagagatc agtaaacaaa ccactccgtt cacgcgcttg tacaatagct gtaatcgcgt
     3481 tttcccctgt gcctttaaca gcgcctaatc catagcgaat cgtttttgca tcggtaggta
     3541 caaaccgata catcgagaaa ttgatatctg gcggtaaaac gttaattcca tttactttgc
     3601 aatcctcata aaaatcatta atcttgtctg tatcattcat ttccgcagac aaacaggcag
     3661 ctagaaaagc cgctggataa tgcgctttga gataagcggt ctggaacgca attaatgcat
     3721 aagccgccgc atgggactta ttgaatccat atcccgcaaa tttctccatg agatcgaata
     3781 gcttaatggc gttctgttca gtcaatccat ttttgattgc accttcaaca aaaatatcac
     3841 gctgctgagc catttcctca acttttttct tacccatggc gcgacgcaat aaatcggctc
     3901 cacccaaact ataaccacca atgacttgcg caatctgcat cacttgttct tgataaatca
     3961 tcacaccata ggttggtcct aatatgggct caagtcttgg atccaaatac tcgacacgtt
     4021 gaatcccatg cttacgctct atgaaatcag gaatcaaatc cataggacct ggcctataaa
     4081 gcgcaactaa ggcaattaga tcttcaaatc gatcgggttt ggctttttgt aataaatctt
     4141 tcatgcctcg cgattcaaat tgaaaaatcc ccaccgcatt gccttttctt aataaagcat
     4201 aggtttcagg atcttccaaa gcaatttgat tcaaagagat gccgccggtt gattcaggct
     4261 cggtttgata tttaatatca tggagctgtt gctgattgat gtcactgatt gcttgatcaa
     4321 gaatcgttag tgtttttaaa cccagaaaat caaacttaac gagtccaatt ttctcaacat
     4381 cgtctttatc aaactgactg accacagatt cactcgcttc cgcacaataa attggacaga
     4441 aatctgtaat ttttccgggc gcaatcagca cgccaccagc gtgcataccc acatttcggg
     4501 taataccttc gagactttca gctaactcta ataaattgcg cacatcctct tcttctcgtg
     4561 cacgcgactc aaactgaggc tcaatagcac gcgctttctt tagtgtcatc cctaattcga
     4621 atggaattaa tttcgcaagt tgatcaacaa aattgaatgg taaatctaaa acacgcccaa
     4681 catcccgtat cactgctttg gcagccatcg tcccaaatgt cacgatttga gagacgcttt
     4741 ccataccata tcggttcttt acatactcga tgacactttc acgacggtct tggcaaaaat
     4801 caatatcaaa atcaggcatc gagatacgtt ctggatttag aaaacgttca aacaacaaat
     4861 catatcgtag cggatcgaga tcggtaattc ctaacgaata agctactaaa gaaccagctc
     4921 cagatcctcg accaggacca acgggaatgt tgttttgctt agcccaatta ataaaatccg
     4981 caacaatgag aaaatagccg gcaaacccca tctggataat ggtgttcact tcaaaattga
     5041 gcctagcttg ataagttgtt aatttgtggt tgaattcatt ttccggtaaa tcttgcaaac
     5101 gctgctttaa acctgataat gcttggttgc ttaaatatgc ttcaatgctg gtcgtgtcag
     5161 gtactggaaa taacggtaat cgattaatac ccagttcaag ctgcaaatta catcgtttcg
     5221 caatttcgac actattggct agcgcttccg gaatatccgc aaacaaagct tgcatttcac
     5281 tttgtgtttt aaaatactgc tgatcagtaa atgcacgggg acgccgccga tctcctagta
     5341 cataaccttc tgcaatacaa actctagctt catgtgcttt ataatctttt gcatccaaaa
     5401 attgaatggc atgcgtggcc acaatcggaa tatttaattg ggcagcgagt atcagcgagt
     5461 ttcgaataaa ccgttcttca tccacccgcc catttcgttg aatctccaga taaaatcgat
     5521 tcggaaataa ttcagaccat tctttactga ctacttgggc ttcctgcaga ttattttgta
     5581 ttatcaattg acccacttcc ccaaaatgtg cgccagatat tgcaattaaa tcttcagttc
     5641 cttctgcaga agaatgaagc catgctttct tcagttctgc cctaccccga aataaatttt
     5701 cacgataggc acgcgacaac aatcggcaca ataatagata gcccgagtgg gtctggcata
     5761 gcaatagtac gcgaaagggg ttgtttcgat cagcttcatt actaatccaa gcatcacagc
     5821 caatgatggg ctttatccct attttctctg cgctttggta aaatttgacg agtccaaata
     5881 aattacccaa atcggtaagc gctagggctg gcatatgatc ttgagctgct cttgctaata
     5941 cttcatcaat ccgaataata ctgtccgtta tcgaatattc gctgtgcagt cgtaaatgaa
     6001 taaaagcagg atctataggc atagtgctac aatttacatc aattaattaa ttatccaagc
     6061 caatattggt cattttacat catgcaaact ttgccagagc tattactccc agcaggttct
     6121 ttagagaaaa tgcggaccgc ctatgctttt ggggctgatg caatttatgc aggtcagcca
     6181 cgctactcgt tacgggctcg caataatgaa ttttcacttg aacaactacg cactggaatt
     6241 attgaggcac atcaacttaa caagaaattt tttgtcgcga gcaatctcat gccccataac
     6301 cgtaaagtca aaacctatat ggcagatatg gagcctgtca tcgatttgaa accagatgcc
//
LOCUS       DAOQLJ010000127         6277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_5305_length_6277_cov_6.378335, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000127 DAOQLJ010000000
VERSION     DAOQLJ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5305_length_6277_cov_6.378335"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..1246
                     /gene="pstC"
                     /locus_tag="PK522_08850"
     CDS             299..1246
                     /gene="pstC"
                     /locus_tag="PK522_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNC41880.1"
                     /translation="MSSKFTKDTGVDPGYSKGSLKYSYKRHLKEKIIELLLLLAAVFS
                     VLVTFAIIYMLVKESYVFFQHVSLKEFLTDTQWTPLFDDAHYGILPLVSGTLVSSVVA
                     LMVALPLGTIIAIYLSEFASFRVREIAKPFLELLGGIPTVVYGYFALLFVTPLLQKIF
                     PNLPGFSLLSAGLVMGIMIIPYVSSMAEDAMRAVPMNLREGSYAMGATRFQTAINVVT
                     PAALSGIAAAYILGISRAVGETMVVAIAAGMQPNLTWNPMDQAATITAYIVQVSLGDL
                     PHGSIGYQTIFAAGLTLLLITLIFNILGHLLRKYYRESY"
     gene            1319..2251
                     /gene="pstA"
                     /locus_tag="PK522_08855"
     CDS             1319..2251
                     /gene="pstA"
                     /locus_tag="PK522_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNC41881.1"
                     /translation="MIEENQTDLRELRSIIVRHKRWDLIFLICGVLALMIAILTFIAL
                     FLNMLIDGLPRLSWEFFTSYPSRRAESAGILSAWVGTTLVMLVTAVSAVPLGIAAGVY
                     LEEYAPKNFFTELIEINVTNLAGVPSIIYGLLALGLFVYQLGLGQSILAAGLTLALLI
                     LPIVIVTTREAIRSIPVAIREGSYALGATKWQTVADHLLPYSAPSILTGIIIGLARAI
                     GESAPIITIGALTFIAFLPNAPVTHEFPFVSFEWLLAPFTVMPIQMFNWVSRPEEAFQ
                     FNAAAAGLVLVIMTLTMNALAIYLRYQMRKNIKW"
     gene            2291..3058
                     /gene="pstB"
                     /locus_tag="PK522_08860"
     CDS             2291..3058
                     /gene="pstB"
                     /locus_tag="PK522_08860"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNC41882.1"
                     /translation="MKNQHKSVVKNLSFHYGDFNALKNIDMVLHDKQVTALIGPSGCG
                     KSTFLRCFNRMHDLYPGNRYEGNIILYPDNENILAPSVDPIEVRMRISMVFQKPNPFP
                     KSIYENVAYGLRIRGIRQRALLDEKIEEALRNAALWDEVKDRLHELAFNLSGGQQQRL
                     CIARALATNPEILLFDEPTSALDPIATASIEELIADLKSKVTILIVTHNMQQAARVSD
                     YTAYMYLGQLIEFDLTDTIFIKPKNKQTEDYITGRFG"
     gene            complement(3068..3793)
                     /locus_tag="PK522_08865"
     CDS             complement(3068..3793)
                     /locus_tag="PK522_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNC41883.1"
                     /translation="MPDHSFKAMILAAGRGERMRPLTDTIPKPLLRVGNKMLIEYHLE
                     NLVLAGFKEIVINHAYLGDMIEKALGDGARYGVAIQYSKETIKLETAGGIANALPYLT
                     ADHRNQPFLVVNGDIYCPIDFSSLIPDLARLNAQSNDDLAHLLLVNNPSHHPEGDFYL
                     SEQGRIIATGQTKLTFSGIGIYRPQLFEQVAINCPTRLAPLLHQAVANHQVSGKFFSG
                     KWVDIGTPDRLNALDQQLRSQRC"
     gene            complement(3801..4811)
                     /locus_tag="PK522_08870"
     CDS             complement(3801..4811)
                     /locus_tag="PK522_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNC41884.1"
                     /translation="METDRLHQLKAWLHNEFPNQHFDLSIASADASFRRYFRVQFEDR
                     SLIAMDAPPQHEDCRPFIKIAQLFSTADVNVPSILSQNLTDGFLLLTDLGNTTYLQQL
                     VAHPSEARAMYSDAIDALIRMQQISQENILLNYDRALLQKELNLFPEWYLTRHKQFNL
                     SQHQSDHLQSTFSAILANNLEQPKVFVHRDFHSRNLMITTPNPGILDFQDAVYGPITY
                     DLVSLFKDAYIQWEEELVLDWVIRYWEKARKARLPVSSDFSIFYRDFEWMGVQRHLKV
                     LGIFARLYHRDNKAGYLNDIPLVLHYLRKTCERYRELHLLKTLLDEIEDAPSPQQVGY
                     TF"
     gene            complement(4921..5568)
                     /locus_tag="PK522_08875"
     CDS             complement(4921..5568)
                     /locus_tag="PK522_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNC41885.1"
                     /translation="METKQLESHFKRSLIAAAASLLLIGCGAEQGKESFSDRVAKRFG
                     GGDAAGLPEPAWKEAAAEHKKAKEAGAASGDKGKEPEKKEPEKAAAEPAKPSPVEATI
                     QATEEIAADVGEKISAVAEDIKTEVSESVNSLTAMATPSADSPTEDLKAVVKPTPEII
                     RQLQQALTDAGYNPGPVDGKSGGKTTEAMKKFQKDNNLTVGQITKETLATLGIAY"
BASE COUNT         1703 a         1260 c         1372 g         1942 t
ORIGIN      
        1 gaagctaaat ctgaagcttc cgatgatgat gaagatgacg aggatgagta aggttggctt
       61 aatccaattc tagcatctgt tgtatcaaag acgaggttct ctgatgacag gaacctcgtt
      121 aaaggttcaa agaaattgaa gaataatctt cgcttctata ggagctccat cctaatctga
      181 gcaatgatca ggttgtcgag gaaggtgctt atttcacaag cttgtaacaa taagcgccta
      241 ctctagctta cataataaaa atatgaattg gctttcccac taacggtaaa tcgataagat
      301 gagttctaaa tttacaaagg atacgggtgt cgatccgggt tattctaagg ggagtctcaa
      361 gtatagttat aagcgacatc tcaaagaaaa gatcattgaa ttgctgctat tacttgccgc
      421 ggtcttttcc gttcttgtca cgtttgcaat catttacatg ctggtaaaag aatcctatgt
      481 tttttttcag catgtatcat taaaagaatt tctgacggat acgcagtgga ctcctttatt
      541 tgatgatgct cactatggca tacttccact cgtttctggc acgctggtaa gttctgttgt
      601 cgcactcatg gttgctctgc cgcttggaac catcattgca atctatctgt ctgaatttgc
      661 ttcatttaga gtgcgtgaaa ttgctaaacc atttcttgag ttactaggtg gcattccaac
      721 tgtggtttat ggttattttg cgctactgtt tgtaacaccg ctgcttcaaa agatttttcc
      781 taatttacct ggattcagct tgctatcagc cggtttggtg atgggaatca tgattattcc
      841 atatgtgagt tccatggctg aggatgcgat gcgagcagtt ccaatgaatt tgcgtgaggg
      901 atcttatgcg atgggagcaa cacgcttcca aacggcaatt aatgtcgtca caccggcagc
      961 tttatcaggg atagcagcag cctacatatt aggtatttcc agagcagtag gtgaaaccat
     1021 ggttgtggca atcgctgcag gtatgcaacc taatctgact tggaatccta tggaccaagc
     1081 tgcaacgata actgcgtata tcgtacaggt aagtttaggg gacttgccac atggcagtat
     1141 cggttaccaa acaatttttg ctgcaggctt aacgttgcta ctcattacac tcattttcaa
     1201 tattctaggc catttattaa gaaaatatta tcgtgagtct tattagtaat gggttaatca
     1261 atgaattctg gaatagcgtc ttgtcgagtg taacgtgttt ttctaattta gtagggttat
     1321 gatagaagaa aaccaaactg atctaagaga attaagatca atcattgttc ggcataaacg
     1381 ctgggatttg atttttctga tctgtggtgt gctcgcgttg atgattgcta ttctgacctt
     1441 tatagccctt tttctgaata tgctcattga tggcttgcca cggttatcct gggagttttt
     1501 tacgtcttac ccttcacggc gggctgaatc ggcgggtatt ttgtcagctt gggtaggtac
     1561 aactttggta atgcttgtga ctgctgtttc agccgttcca ttagggatcg ccgcaggcgt
     1621 ttatttagag gaatacgccc ccaaaaactt ctttacagaa ttaattgaaa ttaatgtgac
     1681 caatcttgca ggcgtaccct cgatcattta cgggttattg gcacttggac tttttgttta
     1741 tcaattaggt ttggggcaaa gcattttagc tgcaggatta acactcgcat tactgatttt
     1801 gccaattgtt atcgtgacaa cccgtgaggc gatacgctct attcctgtag cgattcgtga
     1861 aggatcctat gctctcggcg caacgaaatg gcaaaccgtt gctgaccatt tattgcctta
     1921 ttctgcgcca agtattttaa ccggtattat cattgggctc gccagagcga tcggtgaatc
     1981 tgctcccatt attaccatcg gtgcattgac ttttatcgct tttttaccta acgccccagt
     2041 aacgcatgaa tttccctttg tttcatttga atggttactc gcgcctttta cagttatgcc
     2101 aatccaaatg tttaattggg tatcgaggcc tgaagaagca tttcaattca atgcagccgc
     2161 tgcagggctg gttttagtca ttatgacgct gactatgaat gcactcgcca tttatttgcg
     2221 ctatcagatg cgtaagaata ttaaatggtg acagaaaata ttcaactgat aataataata
     2281 cgaacctctt atgaagaatc agcataaatc ggtagtaaag aatttaagct ttcactatgg
     2341 cgactttaat gcattaaaaa atatcgatat ggttttgcat gataagcaag taactgcttt
     2401 gattgggcca tccggttgtg gaaaatcgac ttttctccgg tgttttaatc ggatgcatga
     2461 tctttatcca ggaaatcgct atgaaggtaa tatcatctta tatcctgata atgagaatat
     2521 ccttgcaccg agcgtcgatc cgattgaagt gaggatgcgt atcagtatgg ttttccagaa
     2581 acccaatcca tttcccaaat ccatttatga aaatgtagcc tatgggctca ggataagagg
     2641 aattcggcag cgtgcgctac tggatgagaa aatcgaagaa gcgcttcgca atgctgcatt
     2701 atgggatgaa gttaaagatc gtctccatga attagccttt aatttatccg gtggacaaca
     2761 gcaaagactt tgcattgcgc gagcgctggc aaccaatcca gaaattttac tgtttgatga
     2821 accaacctcc gcgcttgatc cgattgcaac cgcaagtatt gaagaattaa ttgccgatct
     2881 aaaaagtaaa gtgacgattc tgatcgttac gcataacatg cagcaggctg cccgcgtatc
     2941 ggactatacc gcttatatgt atttaggcca attaatcgag tttgacctga ctgatacgat
     3001 ttttattaaa cctaaaaaca agcaaactga agattatatt accggtcgat ttggttaata
     3061 tgcttcacta gcatcgctgg gatcgaagtt gttgatccag cgcgtttaga cgatcgggtg
     3121 taccgatatc aacccatttt cccgagaaaa acttaccact cacttgatga ttagcaactg
     3181 cctgatgcaa gagtggcgct aatcgtgttg ggcaattgat agctacctgt tcaaacagtt
     3241 ggggtcggta gattccaatg ccactaaatg taagtttcgt ttgaccggtt gcgatgatgc
     3301 gaccttgctc agatagataa aaatcacctt ctggatgatg agaaggatta ttaacgagca
     3361 agaggtgtgc gagatcgtca tttgattgtg cattcaaacg cgctaaatcg ggtatcagcg
     3421 atgagaaatc gatagggcaa tagatatcgc cgttgacgac taaaaaaggt tggttgcgat
     3481 gatcagcagt gagatagggt agtgcatttg cgataccgcc ggctgtttca agtttaatgg
     3541 tttcttttga atattggatg gcgacaccgt aacgtgcgcc atctcccagt gctttttcaa
     3601 tcatatcgcc aagatacgca tgattgataa cgatttcctt gaagccagcg agtacgagat
     3661 tttcaagatg atactcgatc aacattttgt ttcccacgcg aagcaacggt ttgggaatag
     3721 tatccgtgag cgggcgcata cgttcacccc ggcctgctgc tagaatcatt gccttaaaag
     3781 agtgatcagg catacaacga ctaaaaggta taacctactt gttgtggcga tggcgcatct
     3841 tcaatttcat cgagcaaagt tttcaatagg tggagctcac ggtagcgctc acaggttttg
     3901 cgtaaataat gcaagacgag aggaatatca ttgagataac ctgctttgtt atcgcggtga
     3961 taaaggcgtg caaagatacc taataccttc aagtgacgct gtacgcccat ccattcaaag
     4021 tctcgataga agattgagaa atctgatgaa acgggtagcc ttgcttttcg tgctttttcc
     4081 cagtagcgaa tcacccaatc aaggactaac tcctcttccc actgaatgta ggcatctttg
     4141 aacaaggaaa cgaggtcata ggtaataggt ccgtagaccg catcctgaaa atcgagaata
     4201 ccaggattcg gtgtggtaat catgagatta cgcgaatgga agtcgcggtg cacaaatacc
     4261 ttgggttgtt cgagattatt ggcgagtatt gctgagaaag tcgattgcag atgatctgat
     4321 tgatgctgac tcagattgaa ctgtttgtgt cgcgttaggt accattcggg aaatagatta
     4381 agctcctttt gtagcagggc gcgatcataa ttcagcaaga tattttcctg gctgatctgc
     4441 tgcatgcgga tgagcgcgtc gattgcatcg ctgtacattg cccgcgcttc gctaggatga
     4501 gcgactaact gttgtaaata agtggtattt cctaagtcgg tgaggagtaa aaatccatcg
     4561 gttaaattct gagataagat gctgggaaca ttcacatcag ccgttgagaa aagctgagca
     4621 attttgataa agggtctaca gtcttcatgt tgaggcggcg catccattgc aatgaggctg
     4681 cgatcctcaa attgaactcg gaaatagcgt cgaaaactag catcagcaga agcaatcgat
     4741 aaatcaaaat gttgattagg aaattcgttg tgaagccatg cttttaattg atgtagacga
     4801 tcagtttcca caaatacccg atattattgt ttaaaaaaat tgaaagcatt atcaacaaaa
     4861 aataaaggcc agtccatttt tttggactag ccttttatat aaacaaacag aaaagttttt
     4921 ttagtatgcg ataccgagtg ttgctaatgt ttctttagta atctgaccga ctgtcaggtt
     4981 attatctttc tggaattttt tcatcgcttc agtggttttt ccaccgcttt tgccatccac
     5041 agggcctggg ttataaccag catctgtcag cgcttgttgt aactgacgaa tgatttccgg
     5101 tgttggttta acaaccgctt taagatcctc tgttggagag tctgcgctcg gtgttgccat
     5161 ggctgtcaga gaattgacgc tttcactaac ctcagttttg atgtcttcag cgaccgcaga
     5221 aattttttct ccgacatccg ctgcaatttc ttcagttgct tgaatagttg cttcaactgg
     5281 gctgggttta gctggctcag cggctgcctt ttcaggttct tttttctcag gctcttttcc
     5341 tttgtcgcca cttgcagcgc ctgcttcttt cgctttttta tgctcggctg ccgcttcttt
     5401 ccatgccggt tcagggaggc ctgccgcatc tccaccgcca aatcgtttag caacgcgatc
     5461 agagaatgat tcttttcctt gctctgcgcc acaaccgatt aataataacg aagctgctgc
     5521 agcaattagg ctacgtttga agtgtgattc aagttgtttg gtttccatat ttaattctcc
     5581 ttccattttt attgttatta gtaattaatg actcaagtta aacccaaaat atcgaacatg
     5641 ttgaaagtta agattcatat agtttcctta ctcctatgat tgatagacga attttcccaa
     5701 ttaatgattc acttaataca acgctcggtg gttatagctc gttgtattgt tatgattaat
     5761 ttcctaacgg atgcgattaa gtacactaac gctggagact atcacagtat ttgtcaaaaa
     5821 attgtcaaga aggcggagtg ggttgtggat tagaataatc acgctgaatt ccaagcattg
     5881 tttttatcat cagtaatgct aacgggtaag ttgccctaca tttttttgga tttaccgtga
     5941 ttctgagttt atcgatgcaa tcagttattt accatttctc agcagcacca aatcctctaa
     6001 tgatgcggtt tgacatcaaa aataacatcg attagattgc aattaatcga tagcttcgca
     6061 atatttgctt tgcgaaatga gcataaaatt atgagcgata tttttatcag ttatactagc
     6121 gaggatcgtg acttagcaag acaattcccc caaaattatc atccatccgc tcactgagga
     6181 gaataaacca acgggatcac tggaatcgat catattccac cttgcaccac gcttaccttt
     6241 tttactcctt gtgtcgcgtc acatcagcat accattg
//
LOCUS       DAOQLJ010000128         6264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_5336_length_6264_cov_5.600258, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000128 DAOQLJ010000000
VERSION     DAOQLJ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5336_length_6264_cov_5.600258"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..151
                     /locus_tag="PK522_08880"
     tRNA            76..151
                     /locus_tag="PK522_08880"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:110..112,aa:Glu,seq:ttc)
     gene            313..1299
                     /gene="birA"
                     /locus_tag="PK522_08885"
     CDS             313..1299
                     /gene="birA"
                     /locus_tag="PK522_08885"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HNC41886.1"
                     /translation="MKPISFAILKLLSDGQFHSGTQIAQFLGVSRTSVSNALRGIDEF
                     GVTLYRVTGKGYRWINPVQWLDKNAINQYLAEYSQRFRIAIDEVVESTNTILLNTSKA
                     KNYDQSMIDVVVTELQTKGRGRRGRSWFSGLGDSLTFSLSWQFQQGVNFLAGLSLVVG
                     IAIIRTFTSLGIREVALKWPNDLLFNSSKLGGVLIELQGEVQGPATAVIGIGINLTLS
                     DKTKLYVDQDISDLYSITHTPIDRNKLLASLLIEMATVLEIFAAEGFSPFKEEWISYH
                     SYEAREVEVLLPAGLIERGIVTGITEDGSLRLQMESGLKSFHCGEISLRKIG"
     gene            1314..2078
                     /locus_tag="PK522_08890"
     CDS             1314..2078
                     /locus_tag="PK522_08890"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNC41887.1"
                     /translation="MTYLLVIDSGNTAIKWGLADKDKWIDKGVAMQAHRDSLGGVWGK
                     IPEPSQITISNVAGSLAEQSLMLIFKQLQWTAKVNWAFSKSDQCGVKNYYDIPSQLGS
                     DRWAALIAAWSMKQQACLVVSVGTTMTVDILSNQGEFMGGIILPGFDLMQSTLINQTA
                     LLTDSSNGCFQKVPRNTQDAMVSGAIYGLTGAIEQMYSIFTKSLSHSNPKCIISGKGA
                     QTVLPYIDIAGKEWVDDLVLEGLRIISLTDSSAIFT"
     gene            2093..2692
                     /gene="yihA"
                     /locus_tag="PK522_08895"
     CDS             2093..2692
                     /gene="yihA"
                     /locus_tag="PK522_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNC41888.1"
                     /translation="MSILQGIRFYTTVNHLKDLPKTTNIEVAFAGRSNAGKSSAINTL
                     TRRNRLAFVSKMPGRTQHINYFQLNDGNFLVDLPGYGYAKVPQLIREHWRHLLSTYLQ
                     SRSNLVGLILIMDVRHPLTELDQKMLEWFSITNKPVHILLSKSDKLSRNQSMKTLKSV
                     QNFLLEQYPHCTAQLFSNLATVGVEEALQVLSNWFKSTD"
     gene            2894..3904
                     /gene="hemB"
                     /locus_tag="PK522_08900"
     CDS             2894..3904
                     /gene="hemB"
                     /locus_tag="PK522_08900"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNC41889.1"
                     /translation="MSPLSQYPQKRMRRLRRDNFSRNLVRENLLSVDDLIYPVFVLDG
                     QHLKESVASMPGVSRLSIDLLLHQAEQCVNLGIPAMALFPVVDASLKSLTAHEALNPE
                     GLVPRTVRALKKHFPELGVITDIALDPYTSHGQDGLIDENGYVLNDETILVLEQQAVV
                     HAQAGADIVAPSDMMDGRVAAIRKKLDDNQHIHTRILAYSAKYASSFYGPFRDAVGSS
                     ANLKSGNKYTYQMDPANSDEALWEVGLDLQEGADMVMIKPGMPYLDIVRRVKEKFGAP
                     TVVYQVSGEYAMLKAAAINGWLNEEACVLEALLAFKRAGADAIMTYFALDAAGWLKQS
                     GS"
     gene            complement(3926..6247)
                     /locus_tag="PK522_08905"
     CDS             complement(3926..6247)
                     /locus_tag="PK522_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HNC41890.1"
                     /translation="MSLRWLTYPFIALFAVSLIGILLVVFAALVIIPTLPSLEVITDY
                     RPKIPLRIYSAEGLLIGEFGEERRDFVNIRETPVFLKKAILAAEDDRFYQHGGVDYIG
                     VLRAAYSNFSGGGTLQGASTITMQVARNFFLTKEKTLTRKFSEALLAFKIEHSLDKDK
                     ILELYINQIYLGQRSHGFSAAAQTYFGKPLININLAEAAMLAGLPKAPSRLNPVVDLK
                     RSKARQHYVLKRMHELSYISAKEYEEAEKQTILIHRQTRINAMPADHIVEMARQALYQ
                     QYGEDVYTRGLKVYTTIHKADQVAAYEAVRKGILDYDSRYGYRGAEALVDIPPSDANP
                     AKTLISALQEHSDSDDLLIAAVISSNSNQIEAILKNGQPIQISGDGLKIAKKYLATDP
                     QLGNKRIRKGSLIRVQKNNQNQWKITQLPEVEAALISIDANDGKIRALVGGFDFYLNQ
                     FNHVTQAWRQPGSSFKPFIYSASLEKGFTPATIINDAPIYFDAAQTGSKAWEPKNFDG
                     KFGGPIRMRVALAQSKNLASIRILQAIGPRYAQDYIARFGFDSNRHPPYLPMALGAGS
                     VTPLQMASGYAIFANGGYKVEPYFIERVEDDKGNIVQQVDPLVAGQNATQIIDPRNAF
                     LMNSMMQDVIQRGTATAAKQLKRNDLAGKTGTTSNFIDAWFCGYQKDLITVSWVGFDE
                     PKSLGKNETGGKVALPIWINYMEKALKQIPVATYTPPKGIVTAKINPATGLRETAGGM
                     SEYFFQEHLPPVATVNDSDEDLLSNDDFWSQAF"
BASE COUNT         1775 a         1180 c         1414 g         1895 t
ORIGIN      
        1 ttgcatggca tgcaagaggt cagcggttcg atcccgctta tctccaccag tttttgcaat
       61 actttttatg atcttgtccc catcgtctag aggcctagga catcaccctt tcacggtgag
      121 tacaggggtt cgaatcccct tggggacgcc aggtaaattc tattcagata gaaattttct
      181 ctatcgcgct ctatttttgg ctaacttagt tatcaagata gtttataaat acctagcttg
      241 ctcaagttct cgtttgtatt gccatgattc atagcaatac cttaaatcta ttctttaaag
      301 cttagattct cggtgaaacc aatctctttt gcgattttaa agttattgag cgatggccag
      361 tttcattcag gaacgcaaat tgcccaattt ttgggcgttt ctcgtaccag tgtatcgaat
      421 gcactccgtg gtatcgatga atttggtgtg acgctctatc gtgtaaccgg gaaaggatat
      481 cgctggatca atccggtgca atggttggat aagaatgcta ttaatcaata tcttgctgaa
      541 tattcacagc gattccgaat agcgattgat gaagtcgttg aatccactaa tacaattctg
      601 ttaaatacaa gtaaagcaaa aaattatgat caatcgatga ttgatgtggt ggtaacggaa
      661 ttacaaacaa aaggcagggg tcgtcgaggg cgctcgtggt tttctggttt aggtgatagt
      721 ttaacttttt ctttatcttg gcaatttcag caaggagtca attttctggc tggtctaagc
      781 ttagtagttg gaattgcaat aatacgaacc tttacgtcac tcggaataag agaggtcgcc
      841 ctaaagtggc ctaatgattt attattcaat agcagtaaat taggtggggt attgattgaa
      901 ttacagggag aagtacaggg gccagctaca gcagtaattg gcattggaat caatctaacc
      961 ttatcggata aaactaagct ttacgttgat caggatatct cagatcttta ttcgataacg
     1021 cataccccaa ttgatcgcaa taaactactt gcttcattgc tgattgaaat ggcaacggta
     1081 ttagaaattt ttgcggctga aggcttctca ccttttaagg aagagtggat cagttatcat
     1141 agctatgaag caagggaagt tgaagtgtta ttgccagctg ggttgattga aagaggaatt
     1201 gtaactggaa taacggaaga tggttcgtta cgactacaaa tggaatctgg attgaaaagc
     1261 tttcattgtg gtgagattag cttgcgtaaa attggttgag aattttttat tcaatgactt
     1321 atttgctcgt tatcgattca ggtaatactg ccataaagtg gggattggcg gataaggata
     1381 aatggattga taaaggggtt gccatgcagg cgcatcgcga ttcactggga ggtgtctggg
     1441 ggaaaatacc ggaaccatca caaattacga tctccaatgt ggctggatca ctcgcagaac
     1501 aatcgctgat gctgatattt aaacagcttc agtggacagc aaaggtaaat tgggctttct
     1561 caaaaagtga tcaatgtggg gttaagaatt attatgacat tccttctcaa ctaggaagtg
     1621 atcgatgggc tgctttaatt gctgcatgga gcatgaagca acaagcctgt ttagtcgtga
     1681 gtgttggaac caccatgacg gttgatattc tttctaatca aggtgaattt atgggtggaa
     1741 ttattttgcc tggatttgat ttgatgcaaa gcacgttaat aaaccaaact gcgttattaa
     1801 ccgattccag caacggttgt tttcaaaagg taccgcgtaa tacccaagat gccatggtga
     1861 gcggcgcaat ttatgggtta actggcgcaa tcgagcagat gtatagcata tttacaaaga
     1921 gcttaagtca ttctaatcct aaatgtatta ttagcgggaa aggtgctcaa acggtgttgc
     1981 cttatatcga tattgctggc aaagaatggg tagatgatct agtgctagaa gggttaagga
     2041 ttatttcatt gacagattcg agtgctatat ttacttgagc gatgatcaga tgatgtcaat
     2101 tttacaaggg ataagatttt atacgacggt caatcacctt aaggatcttc caaaaacaac
     2161 aaacatagaa gtggcatttg ctggtcgctc aaatgcggga aaatcaagtg cgattaacac
     2221 attgacgagg cgtaaccgac ttgcttttgt cagtaaaatg cctgggcgca cccagcatat
     2281 caattacttt caattgaatg atggtaattt tctggttgat cttccaggtt atggatacgc
     2341 aaaagtaccg caattgatac gtgagcattg gaggcattta ctgagtactt atttacaaag
     2401 ccggtctaat ttagttgggt tgatattgat tatggatgtg cgccatccac tgactgagct
     2461 tgatcaaaag atgcttgagt ggttttcgat cacaaataaa ccggttcaca ttttgttgtc
     2521 taagtcagat aagttgtcac gaaaccaatc aatgaaaacc ttaaaaagcg tacaaaattt
     2581 tttgttagaa caatatccgc attgcacagc ccaattattc tctaatttag caactgtcgg
     2641 ggtcgaggaa gcattgcagg tattatcaaa ttggtttaaa tcaaccgact aattttacag
     2701 tatgatgctt tactgtcaca ttaggcaaaa aaaacccccg gtcaaagggg aaaaaccggg
     2761 ggagttgcct tatctcaaag gcctcgcctc agggagggaa ggcgagagac gatcatcata
     2821 tgcttgcgtc taagcatatg agacgaaatt ctctgttaag ttcattttat ttcaattttt
     2881 ttcttaaatt gctatgtcac cattaagtca atacccacaa aaaagaatgc ggcgattacg
     2941 tcgtgataac ttttcaagaa atttagttag agagaattta ttaagtgttg atgatcttat
     3001 ttaccctgta tttgtgttag atggacaaca tctcaaagaa tcagttgctt caatgccggg
     3061 tgtctcaagg ctaagtatcg atctgctatt gcatcaagct gagcaatgtg tgaatttagg
     3121 tattccagca atggcgttat ttccggttgt tgacgcctca ttaaagagct taactgctca
     3181 tgaagcacta aaccctgagg ggttagtacc taggacggtg agagcattaa aaaagcattt
     3241 tccagaatta ggtgtgatta ctgatatcgc actggaccct tatacgagtc atggacagga
     3301 tggattgatt gatgagaatg gttatgtact aaatgatgag acaattttag tgttagagca
     3361 gcaagccgta gtacatgccc aagccggggc tgatatcgtt gcaccttctg atatgatgga
     3421 cggtagggtg gctgcaattc gaaaaaaatt agatgataat cagcacatcc atacgcggat
     3481 tcttgcctat tcggcgaaat atgcgtcaag tttttatggg ccattcaggg atgcggtggg
     3541 ttcgtcagcg aacttgaaat caggtaataa atacacttat caaatggatc ctgccaattc
     3601 agatgaagca ttatgggaag ttgggctaga tttacaagag ggtgcagata tggtgatgat
     3661 taaaccaggt atgccctatc tggatattgt tcgcagagtg aaagagaaat ttggtgctcc
     3721 caccgttgtt tatcaagtga gtggtgagta tgcaatgctc aaagcagcgg caatcaatgg
     3781 ttggctaaat gaggaagctt gtgttctaga ggcgctgctt gcttttaaac gagctggtgc
     3841 tgatgcgatt atgacctatt ttgcattaga cgcagccggt tggttaaaac aaagcgggtc
     3901 gtaggaagca attgaatagg agaatctaaa aggcttgact ccagaaatcg tcatttgata
     3961 aaaggtcttc atcactatca ttaactgttg ccacaggagg aaggtgctct tgaaaaaagt
     4021 attcgctcat tcctcctgct gtttctctta acccagtagc tggatttatt tttgcggtaa
     4081 caattccttt tggaggggta taggtcgcaa ctgggatctg ttttaacgct ttctccatat
     4141 aattaatcca gatcggtaac gctaccttac ccccggtttc attttttcca agtgacttcg
     4201 gttcgtcaaa tcccacccag gaaacggtaa tcaagtcttt ctggtatccg caaaaccatg
     4261 catcaatgaa attactggta gttccagttt ttccagcgag atcattacgt ttaagttgct
     4321 ttgctgctgt cgcagtaccc cgttgtatga catcttgcat catactgttc atcagaaacg
     4381 cattacgtgg gtcgataatt tgtgttgcat tttgacctgc cacaagtgga tcgacttgct
     4441 gaacaatatt gcctttatcg tcttcaacgc gctcaatgaa atagggctct accttatatc
     4501 ctccattcgc aaatatggcg taacccgatg ccatttgtag aggagttacg gagcctgccc
     4561 ctaatgccat gggtaaatag ggtggatggc ggttgctatc gaagccaaaa cgtgcaatat
     4621 agtcttgtgc atagcgtggg ccaatggcct gtagaattcg aatcgatgca agatttttgg
     4681 attgcgcaag cgcaacacgc atgcgaatcg ggccaccaaa cttaccatcg aaattcttgg
     4741 gttcccatgc tttactccct gtttgagcgg catcaaaata aataggagca tcattaataa
     4801 tggtggcagg tgtaaatcct ttttctagag atgctgaata gatgaagggt ttaaagcttg
     4861 atccaggctg tctccatgcc tgagtaacat gattgaattg atttaagtag aaatcaaaac
     4921 caccaaccag cgcacgtatt tttccatcgt ttgcatcaat tgaaatcagc gcagcctcaa
     4981 cctcaggtaa ctgcgtaatt ttccattggt tctgattgtt tttctgaacg cgaatgagtg
     5041 aacccttgcg aattctctta ttccctaatt gtggatcagt agcaaggtat ttcttggcta
     5101 tttttaagcc atcacccgaa atttgaatgg gttgtccgtt ctttagaatt gcttcaatct
     5161 gattggaatt tgaagatatt actgctgcga ttagtagatc atcactatcg ctatgttctt
     5221 gtagcgcgga tataagcgtt tttgccggat tagcatcact gggtggaata tctaccaatg
     5281 cttctgcacc tctataacca taacgtgagt cgtaatctaa aattccttta cggacagcct
     5341 cataagctgc cacttgatca gccttgtgaa tggtagtgta gacctttagt ccacgggtat
     5401 acacatcctc tccatactgt tgatacaatg cttggcgtgc catttcgacg atgtgatcgg
     5461 caggcatggc attaatacga gtttgtcggt gaattaaaat agtttgtttt tcagcttctt
     5521 cgtattcttt ggctgaaata tagctaagct catgcattct ttttagcacg tagtgttgcc
     5581 gtgcctttga tcgtttgagg tcaacgaccg gatttaatcg tgaaggcgct ttaggcaacc
     5641 ctgctagcat tgcggcttct gcaagattga tattgataag tggcttccca aaataggttt
     5701 gtgccgctgc tgaaaagccg tggcttcttt gccctaagta tatttgattg atataaagtt
     5761 ctagtatctt gtctttatca aggctgtgct caatcttaaa ggcaaggagc gcttcactaa
     5821 attttcgagt tagggttttt tctttagtta gaaaaaaatt ccgagcaact tgcatggtga
     5881 tagtactcgc accctgtaat gtgcccccac cagaaaaatt ggagtatgca gctctcagta
     5941 cgccaatata atcgacccct ccgtgttgat aaaagcgatc atcctctgca gctaaaatcg
     6001 ctttttttag aaataccggt gtttcacgaa tattgacgaa atctcgtctt tcttctccaa
     6061 attctccaat aagcaatccc tctgcactat aaatgcgtag cggtattttc ggtcgataat
     6121 cagtaatgac ttcaagagag ggtaaggtcg ggatgatgac gagagcagca aacacaacta
     6181 acaaaatgcc aatcaaactg acggcgaaaa gcgcaataaa gggataagtt aaccaacgga
     6241 gtgacatgaa agggattgct taat
//
LOCUS       DAOQLJ010000129         6226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_5384_length_6226_cov_5.913304, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000129 DAOQLJ010000000
VERSION     DAOQLJ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6226
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5384_length_6226_cov_5.913304"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PK522_08910"
     CDS             <1..733
                     /locus_tag="PK522_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HNC41891.1"
                     /translation="GMAQILSEVVGGTAMMAFWYHFAILFEALFILTAVDAGTRAGRF
                     MLQDLLGSFIPAMKKTESVPASLVATFICVSGWGYFLYQGVVDPLGGINTLWPLFGIS
                     NQMLAGIALILCTCVLFKMKQDRYAWVTMIPVTWVCVCTLTAAWQKIFHDNPRIGFLA
                     HAEKYQEAVMQGLVLAPAKSIDQMQQVIINDYVNATLAGLFMAVLISMLWFGLRTVLQ
                     ARTNQHPTVQEAPFELLPVTVPTGK"
     gene            750..944
                     /locus_tag="PK522_08915"
     CDS             750..944
                     /locus_tag="PK522_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HNC41892.1"
                     /translation="MQFSITEVCNRVAQSLRLMVGVPEYSQYVAHMKTAHPDRTIMTE
                     VQFYRERQEARYGGKVSRCC"
     gene            complement(954..1472)
                     /gene="msrB"
                     /locus_tag="PK522_08920"
     CDS             complement(954..1472)
                     /gene="msrB"
                     /locus_tag="PK522_08920"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNC41893.1"
                     /translation="MKRRAVLKTLATASMFPFMAACWRIPEIGEAANPITPLKKSEKE
                     WRELLSQRAYQILFEEETERPESSNLVYEDREGTYLCAACYLPLFESQHKYESWTGWP
                     SFTQPIAGHVATQLDFKLIKPRVEYHCVRCGGHQGHVFQDGPPPRGERWCNNGIALKF
                     VSKDEPLPALRT"
     gene            complement(1483..1662)
                     /locus_tag="PK522_08925"
     CDS             complement(1483..1662)
                     /locus_tag="PK522_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41894.1"
                     /translation="MDWMQIVSALALGLFVVFLFPTAINMMKNSPKGSAADWQSVIVP
                     MVIIILFIMFLIQLV"
     gene            complement(1756..2427)
                     /locus_tag="PK522_08930"
     CDS             complement(1756..2427)
                     /locus_tag="PK522_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HNC41895.1"
                     /translation="MNRSFTTVSQSSTSALATNKVLRNTYMLLSLTLLFSGLTAGLSM
                     FLNMPPMTYMISVIGGMVLAMFVLPRFAHSSAGIGIVFLITGLLGFGLGPMLTMYASL
                     PNGGNIITLSLGGTGVIFLGLSAYALATRKDFSFMGGFLVVGFLLVLIAAIANIFLQI
                     PAMSLAISSVVILIMSGFILYDTSRIIHGGEDNYVLATIGLYMTIFNIFISLLQILGI
                     MGKDD"
     gene            complement(2710..4578)
                     /locus_tag="PK522_08935"
     CDS             complement(2710..4578)
                     /locus_tag="PK522_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNC41896.1"
                     /translation="MTLAMQGKLVAQDPNDPPASELLKAIEAEKQRLHVTSSLRKQGS
                     SEGKNLDSLLRGNDGMKIKPLPPIKPKEVPYELPCGWEWVRLGNICSYIQRGKGPNYV
                     DFSMHRVVSQKCVRWYGLDIGPARYIDPISLEKYESFRFLRAGDLLWNSTGTGTIGRA
                     CIFPQELEKSKIVVDSHVTVVRSIKINSLFLWRWIQSPIVQNDIESSASGTTNQIELN
                     TTTVVNQLMPLPPLPEQHRIVAKIDPLMAKCDELEKLRKERQEKRLAVHAAAIKQLLD
                     NPPSFPRRRESSSTEELESHSPSTRGQACGDDEKELGSDGVSDSSWDFIQQHFGELYT
                     VKENVAELRKVILQLAVMGRLVPQGPNDPPASELLKVIEAEKQRLHATSSLRRQGSSE
                     GKNLDSRLRGNDGIKIKSLPLIKPEEVPYELPLGWEWVRLGNLTQVITKGSSPNWQGV
                     QYTDKDKGILFITSENVGSYELLLEKRKYVEEKFNLIEPRSILKKRDILMNIVGASIG
                     RTAMFDLDEVANINQAVAIIRLIKKLDHEYFLRFFNSQTCLSYMFDKQVDNARPNLSM
                     GNIALFAIPLPPLPEQHRIVAKIDQLMALCDKLEQQINAANHKQSELLNAVMASMS"
     gene            complement(4674..5180)
                     /locus_tag="PK522_08940"
     CDS             complement(4674..5180)
                     /locus_tag="PK522_08940"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNC41897.1"
                     /translation="MLPDGFLFGEGVKTTLKRELLEEFNLHTIVRLPKGVFSPYTSIA
                     TNLLFFEKSGSTQEVWFFEHPYPEGYKSYSRSKPLTIAEFEREKSWWGGSARKDRKTT
                     EYAWKVSAQDIAERNYNLDCKNPHEVEVNHRAPEELMAEYQQIVKQLEDAQQDLKAEL
                     MTCLGKKS"
     gene            complement(5236..6105)
                     /locus_tag="PK522_08945"
                     /pseudo
     CDS             complement(5236..6105)
                     /locus_tag="PK522_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
BASE COUNT         1522 a         1450 c         1520 g         1734 t
ORIGIN      
        1 gggtatggcg caaattttgt ctgaggttgt cggtggtaca gccatgatgg cgttctggta
       61 ccactttgcg attctattcg aagcgctgtt tattttgacg gcagttgatg cggggaccag
      121 agcaggacgt tttatgttgc aagatttact cggctctttt attcctgcca tgaaaaaaac
      181 cgaatcagtt ccggctagtt tagtggctac ttttatttgt gtgtcaggat ggggatattt
      241 tctataccaa ggggttgtcg atcccctcgg tggcattaat accttgtggc cattatttgg
      301 tatttccaat caaatgctag ctggtatcgc attgattcta tgtacctgcg tactgtttaa
      361 gatgaaacag gatcgctatg cctgggtgac tatgatccca gttacctggg tttgtgtatg
      421 cacgttaacc gcagcgtggc agaaaatctt tcatgacaat ccgcgcattg gttttctggc
      481 ccatgcggaa aaataccagg aggctgtaat gcaaggattg gtactcgcgc ctgccaaatc
      541 gatcgaccaa atgcagcaag tcattattaa cgattatgtc aatgcaacgt tagcggggtt
      601 gtttatggca gtcctgatca gtatgttgtg gtttggtctc cgaaccgtgc tgcaagcgcg
      661 tactaatcaa catccaacgg tacaggaggc gccttttgag ctgttaccgg ttaccgttcc
      721 gaccggaaaa taattaaagg agtaacacaa tgcaatttag catcacagaa gtttgtaatc
      781 gcgttgctca aagtctgcgc ttgatggtgg gtgtacccga atatagccaa tacgtagccc
      841 atatgaaaac agcgcatcca gatcgaacga ttatgaccga ggtgcaattt tatcgtgaac
      901 ggcaagaagc gcgttatgga gggaaagtta gccgctgttg ctagcggttg gtatcaagtt
      961 cttagcgctg gcaagggttc gtctttagat acaaatttga gcgcaatccc attattgcac
     1021 caacgttcac cgcgtggcgg agggccatcc tggaatacat gaccttgatg cccgccgcat
     1081 cgaacgcagt gatattcaac acggggctta atcagcttga aatcgagttg ggttgccaca
     1141 tgacctgcaa tcggctgagt gaaactcggc cagcctgtcc aactttcata tttgtgctga
     1201 ctttcaaata gtggtaaata gcacgctgcg caaagataag tcccttcacg gtcttcataa
     1261 accagattgc tggattcggg tcgttcggtt tcttcctcga agagaatttg ataagcgcgc
     1321 tgtgagagta attcacgcca ttctttctca gatttcttga gtggcgtgat gggattggca
     1381 gcttcgccaa tttcaggaat tcgccagcaa gcagccatga acggaaacat actcgcagtg
     1441 gctagtgttt ttaatacagc gcgccgtttc attctaatgc actcaaacta gctggattaa
     1501 aaacataata aataaaataa tgatgaccat cggcacaata actgattgcc aatctgcagc
     1561 gctgccttta gggctgttct tcatcatatt gatagcagtt ggaaataaaa aaactacaaa
     1621 caaccctaac gctaatgcag agacaatttg catccaatcc atactcaggt ttttccttta
     1681 ttattgagca caaaaaaaca aaccccggat gaaccggggt ttgtttgtct aacataggta
     1741 acttatcgac aagaatcagt cgtctttacc catgatgcct aaaatttgca acaagctaat
     1801 gaagatattg aaaatcgtca tgtaaagacc gattgttgcc aacacatagt tgtcttcacc
     1861 gccatgaata atgcggctgg tgtcgtaaag aataaaacca ctcatgatca gaataaccac
     1921 agatgagatc gcgagtgaca tcgctgggat ttgcaagaaa atatttgcaa tcgcagcaat
     1981 taatactagc aaaaagccca cgaccaagaa tccacccata aaactaaaat ctttccgggt
     2041 agctagcgca taggctgaca atcccaggaa aatcacaccg gttccaccta gcgacaaggt
     2101 aatgatattg cccccatttg gcagtgaggc atacattgtc aacatgggac ctaacccaaa
     2161 ccccagcaaa ccggtaatca gaaaaacaat accaatgccc gcgctagaat gagcaaaacg
     2221 cggcaacaca aacattgcaa gcaccatgcc gccgatcaca gaaatcatat aggtcattgg
     2281 cggcatattc agaaacattg agagtccagc agtcaatccg ctgaacaata atgtcaacga
     2341 tagaagcatg taggtattcc gtaatacctt gttagtagct aatgcagacg tcgaactttg
     2401 tgaaacggtt gtaaagcttc gattcatttt gtgacctcca acaaaaacat aaatgtgaat
     2461 attatcattg tatttaagca cacgatatcg attaaatacc gtcttctgcg ctcatctctt
     2521 gttaacgtag gttagaccta cttttttaat tatagatcaa ttattttcga tgatttagaa
     2581 aaataccgac ccaagagccc agtttaaatg atttttgagc tcagataaaa gtgctattac
     2641 gatgaataag ataacaaatt catcgacttt tgttttaatt tttctcagaa tgcaatgtgc
     2701 taaacgttat catgacatgc ttgccatgac cgcattgagc aattcgcttt gtttatggtt
     2761 ggcagcgttg atctgctgtt ctagcttgtc gcacaaggcc attaactggt cgattttggc
     2821 gacgatgcgg tgctgttcgg ggaggggtgg gagaggaatt gcaaataatg cgatgtttcc
     2881 catactaagg ttggggcgcg cattatcaac ctgcttatca aacatatagc tcaagcaggt
     2941 ttggctgttg aaaaacctca agaaatattc atgatccaat tttttaatta aacgaataat
     3001 tgctactgcc tgattaatat tcgccacttc atcaagatcg aacatagcag ttcgcccgat
     3061 agatgcgcca acaatattca tcaaaatgtc tctttttttg aggattgatc taggttctat
     3121 caggttgaat ttttcttcca catacttcct tttttctagt agtaattcat atgaacccac
     3181 attctcgcta gtgatgaaaa gaatgccttt gtctttatca gtatattgca ccccctgcca
     3241 attcggcgag gagcctttag ttattacttg agtcagatta cccaacctca cccactccca
     3301 ccctagcggt agttcataag gcacttcctc cggtttgatg agcggcagtg acttgatctt
     3361 tatgccgtca ttcccgcgaa ggcgggaatc caagtttttt ccttcactgg atccctgcct
     3421 gcgcagggat gacgtggcgt gaagtcgctg tttctcggct tcgatgactt tcaacagttc
     3481 actggcgggc gggtcgttgg ggccctgggg gacgaggcgg cccatgacgg cgagctgaag
     3541 gatgactttg cgcagttcgg cgacgttttc cttgacggtg tagagttcgc cgaaatgttg
     3601 ttggataaag tcccaactgc tatccgaaac accatcactc ccgagctctt tttcgtcatc
     3661 cccgcatgct tgtcctcgtg tagacgggga gtgggattcc aattcttccg tgctactgga
     3721 ttcccgcctg cgcgggaatg acggggggtt gtcgagcagt tgcttgatcg cggcagcgtg
     3781 gacagccaac cgcttctcct gccgttcctt gcgtagcttt tccagttcat cgcatttcgc
     3841 catcaacggg tcaattttgg cgacgatgcg gtgttgttcg gggaggggcg ggagaggcat
     3901 tagttgattg acaacagttg tagtattcaa ctcaatctga tttgttgtgc ctgatgctga
     3961 actttcaata tcattctgaa cgataggact ttgaatccaa cgccacagaa acagtgagtt
     4021 aattttgatt gagcgaacga ctgttacatg ggaatctact acgattttag atttttcgag
     4081 ttcttgtgga aagatgcacg ctctaccaat ggttcctgta ccagtcgaat tccacaaaag
     4141 atctccagct cttaaaaatc gaaacgattc atacttttcc agtgagattg gatcaatata
     4201 tcttgcaggt ccgatatcaa gaccatacca tctgacacat ttttgtgaaa ccacgcggtg
     4261 catcgaaaaa tcgacataat taggtccttt tcctcgctgg atgtatgaac aaatattacc
     4321 caacctcacc cactcccacc cacacggcaa ttcataaggc acctccttag gcttaatcgg
     4381 gggcagcggc ttgattttca tgccgtcatt cccgcgtagg agggaatcca ggttttttcc
     4441 ttcactggat ccctgcttgc gcagggatga cgtgacgtgg agtcgctgtt tttcggcttc
     4501 aatggctttc agcaattcgc tggccggtgg atcgttcgga tcttgcgcca ctaatttacc
     4561 ttgcatcgcc agcgtcaaga tcagttctca aagttttttg atgccgtctg gggcggcaaa
     4621 ggcggtgtcg aagtgttgtt ccagaagctc gttcacgcca aggcgtgagg tcatcatgat
     4681 tttttcccca ggcaggtcat cagttcggct ttcaagtcct gctgcgcgtc ttcgagttgt
     4741 ttgacgattt gttggtattc cgccatcagt tcttccggtg cgcggtgatt gacttccact
     4801 tcatgcggat tcttgcaatc gaggttgtaa ttgcgttcgg caatgtcttg cgcgctgact
     4861 ttccaggcgt attcggtggt ttttcggtct ttgcgtgcgc tgccgcccca ccagcttttt
     4921 tcccgctcga attcggcaat ggtcagcggt ttggagcggg aataggattt atacccctcg
     4981 ggataggggt gctcgaaaaa ccagacctcc tgggtcgatc cgcttttctc gaagaacaac
     5041 agattggtgg cgatgctggt gtatggactg aatacgcctt tgggcaaacg cacgatggtg
     5101 tgcaggttga attcttccag caattcgcgt ttgagcgtgg tcttgacacc ttcgccgaac
     5161 agaaaaccat ctggtagcac gcaccaccgc ggctttgccg ccgtcgtgtt tcaataggtg
     5221 catgatcaat gccatgaaca agtcggcggt ttcgcgagtc tggtatttgc gcgggaagtt
     5281 gttttcgatg ccgtcttctt ccatgccgcc aaacggcggg ttggtgacga tcaggtcgac
     5341 ccggtcgcgc gggccgtaat ctctgagcgg acggctcagt gtgttgtcgt ggcgaatgtt
     5401 ggtgggcacg tcgatgccgt gcagcatcat gtttgtcatg gccagcatgt gcggcagcgg
     5461 tttcttttcc accccggaaa tgctttcctg taaccgcgtg cggtcgccgg ggtttttgat
     5521 ctgctgcttc aggtgttcga tggtgcaggt gaggaaaccg ccggtgccgc aggccgggtc
     5581 gagaatgatt tgcccgagct gcgggtcgag catgtcgacc atgaactgag taacggcgcg
     5641 aggggtgtag tattcaccgg cattgccggc cgattgcagg tcggcgagaa ttttttcata
     5701 aatgtcgttg aacaggtggc ggtcactgga tttgttgaaa tcgacatccg cctcgatggt
     5761 gttgatcacc tggcgcagca atgtgccgga tttcatgtag ttgtaagcat cctcgaacac
     5821 cgagccgacc actttaccgt gcggcgacac accggcagcc gtcgccagtt ttttcagtgc
     5881 cggaaaaaga tcgttgttga cgaaatcgat caactcatcg ccggtcatgc cctccgcatc
     5941 ccttgcccag ttgctccagc ggaagcggct gggcagcggc gatttgtagc cgggaatggt
     6001 gagctgccat tcttgttcct tgtcgtcgaa gattttcaga aagatcagcc aaaccatctg
     6061 gctgatgcgc tgtgcatcgc cgtcgacacc aacgtctttg cgcatgatgt cctgaataga
     6121 tttaaccagg gtattgaggg acatggttgt gttatctcca gttcttctat cttgaatcgc
     6181 tccgggtttt ccggagacct tttagtttga gtcaagccac attagc
//
LOCUS       DAOQLJ010000130         6212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_5404_length_6212_cov_5.640084, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000130 DAOQLJ010000000
VERSION     DAOQLJ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5404_length_6212_cov_5.640084"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /gene="sucA"
                     /locus_tag="PK522_08950"
     CDS             complement(<1..543)
                     /gene="sucA"
                     /locus_tag="PK522_08950"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF027404.2"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNC41898.1"
                     /translation="MMRQFIENSFLFGTNAPFVEDLYERYLSSPESISPEWRSFFDNL
                     QQTIAISTKDRPHSLIIQSLVNPVNKIASIQDKSLTQPLFNQPYDETKERKQIAVLQL
                     INMYRFLGVRCAKLDPLNHQPLLDVPELDPAFHGLTANDLDTNFNTGSLVGPEHAPLR
                     TILQILQQTYCNAIGAEYMFI"
     gene            complement(689..1990)
                     /gene="gltA"
                     /locus_tag="PK522_08955"
     CDS             complement(689..1990)
                     /gene="gltA"
                     /locus_tag="PK522_08955"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNC41899.1"
                     /translation="MTEQRKATLRLGENTEPIEFPILSGNMGPDTVDIRTLYAKTGLF
                     TYDPGFLSTASTKSSITFIDGDKGILLYRGYPIEQLAARCDFMEVCHLLLTGELPTDP
                     QKQHFVSTIKSHTMLHEQLVRFYTGFRRDAHPMAVMVGVVGALSAFYHEAMDISDVLH
                     REQSAFRLIAKLPTIAAMAYKYNIGQPFIYPRNDLSYAANFLHMMFATPAEKYEPNPV
                     IVRALDRILILHADHEQNASTSTVRLAGSSGANPFACISAGIACLWGPAHGGANEACL
                     NMLEQIGDVSRINEYIKRAKDKTDGFRLMGFGHRVYKNFDPRAKLMRETCHEVLNEME
                     LHNNRLFKLALELERIALEDDYFIAKKLYPNVDFYSGIVQKALGIPTSMFTAIFAMAR
                     TVGWVAQWNEMIADPDQKIGRPRQLYTGTGLRDIPADKINN"
     gene            complement(2078..2314)
                     /locus_tag="PK522_08960"
     CDS             complement(2078..2314)
                     /locus_tag="PK522_08960"
                     /inference="COORDINATES: protein motif:HMM:NF015870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HNC41900.1"
                     /translation="MTKRIERIRWRCRRGLLELDLLLQRFLNKYDSQLNEQQIDQFES
                     LLSLSDNDLLAIICSNPETVDKSLKRLIQLIQRS"
     gene            complement(2311..3006)
                     /locus_tag="PK522_08965"
     CDS             complement(2311..3006)
                     /locus_tag="PK522_08965"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HNC41901.1"
                     /translation="MRFLISRFDPDKDEKPYMQDYEIELEPTDKMLLDALIRIKSIDD
                     SLSLRRSCREGVCGSDAMNINGRNGLACITPIATLKEPIEIRPLPGLPIIRDLIVDMS
                     QFFKQYHSIKPYLVNHDPPPETERLQSPSERAELDGVYECILCACCSTSCPSFWWNPD
                     KFVGPAGLLQAYRFLADSRDQATTERLDYLEDPYRLFRCHSIMNCVDVCPKGLNPTNA
                     IGKIKGMMLNKVV"
     gene            complement(3125..4537)
                     /gene="thrC"
                     /locus_tag="PK522_08970"
     CDS             complement(3125..4537)
                     /gene="thrC"
                     /locus_tag="PK522_08970"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNC41902.1"
                     /translation="MRYISTRGGMSPQNFSEILLSGLAQDGGLAVPESYPRVDAAELE
                     SWRYLSYPELAFEILSRFMDDIPTANLRDIITRTYTSKLFGSADITPIKTLEPGLHIL
                     CLSNGPTLAFKDIAMQFLGNLFEYTLAKTGSELNILGATSGDTGSSAEYAMRGKQRIR
                     VFMLSPYGKMSRFQTAQMFSLQDENIYNIAIQGVFDDCQDIVKAVSNDHPFKKGHHIG
                     TVNSINWARIAAQIVYYFKGYFAVTQSNDEFVSFSVPSGNFGNIFAGHVARMMGLPIK
                     HLILATNENDVLDEFFRTGLYRPRTTSETHHTSSPSMDISKASNFERYVFDLSERDSA
                     RIKELWQSVENGNGFDLFGTAIWAKAKDLGFLSGSSHHSNRIETIRETYKKYGVLVDT
                     HTADGIKVGLENRESDIPLICLETALPVKFSDSIQEAIGKEPEPIPGFEDLEKLPQRF
                     IVMDKDVNEVKQFISSKVRS"
     gene            complement(4599..5912)
                     /locus_tag="PK522_08975"
     CDS             complement(4599..5912)
                     /locus_tag="PK522_08975"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNC41903.1"
                     /translation="MKSINVGLLGIGTVGGGTFTVLKRNQEEIARRVGCNIVIQMVAD
                     KDIEKARHIVGSQTIVTQNAQEIVANPDIEIIIELIGGQTIAKELILQAINNGKHIVT
                     ANKALLANHGSEIFSAAQQKGVIVAFEAAVAGGIPIIKALREGLTANRIEWIAGIING
                     TSNYILSEMREKGLAFSTALATAQRLGYAEADPTYDIEGIDAAHKITIMAAIAFGIPV
                     RFEKAYIEGISQLNNEDIRNAEELGYRIKLLGITKRVEKGIELRVHPTLIPIKRLIAN
                     VEGVMNAIVVKGDAVGPTLYYGAGAGAEPTASSVIADLVDVARQLTVDPKHRVPYLAF
                     PPDSLSDTPIVSMEDVQTAYYLRLQVIDKPGVLADITRILADADISISAMIQKEHAEE
                     EDRVNIIMLTHTTQEKNINRAIDKIEKLPSMTDKVVRIRLEELNK"
     gene            complement(6008..>6212)
                     /locus_tag="PK522_08980"
     CDS             complement(6008..>6212)
                     /locus_tag="PK522_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HNC41904.1"
                     /translation="IYPIQDDERFVLDLLLEEKVLVVQGTGFNWLHPDHFRVVFLPNS
                     DDLTEAISRIARFLDRFRKRYKT"
BASE COUNT         1767 a         1232 c         1308 g         1905 t
ORIGIN      
        1 aatgaacata tattcagcgc cgatcgcatt gcaataagtt tgctgaagaa tttgtaatat
       61 ggttcgaagt ggcgcatgtt ccgggccgac tagcgacccg gtgttaaagt ttgtgtctag
      121 atcattcgct gttaatccat gaaaagcagg gtcaagttct ggaacatcga gtagcggctg
      181 atggttgagt gggtcaagtt tggcacatct tacacctagg aatcgataca tattgatcaa
      241 ctgcaacact gcaatttgct tacgttcttt ggtttcatcg taaggctggt tgaatagtgg
      301 ttgtgtaagg cttttgtctt gaattgaggc tatcttatta actgggttga ctaatgattg
      361 aatgatcaag gagtggggtc gatcttttgt gctgattgca atggtttgtt gaagattatc
      421 aaagaagctg cgccattctg gagagataga ctcgggacta cttaagtagc gctcatagag
      481 atcctcaaca aaaggcgcat tggtaccaaa cagaaatgaa ttttcgataa attgtctcat
      541 catgcaccaa cccatttaat ccagctgaaa atatttcaca ataaagtgaa gttattttca
      601 aactatgttg aaaaccgatt tataaaaata acagcatgtt tggcatgctg cttcaaatta
      661 attttgaggc attcttatcg tataaaaatt agttgtttat tttatcagcg gggatatcgc
      721 gaagcccggt acccgtatag agttgtcgag gtcgacctat tttctgatct ggatccgcaa
      781 tcatttcgtt ccattgtgct acccaaccga ccgttcgtgc cattgcaaag atagcggtaa
      841 acatcgatgt tgggataccg agtgcttttt gcacgatgcc agaataaaaa tcaacattgg
      901 ggtaaagttt cttcgcgata aagtagtcat cttcaagtgc aattctttct agttctaaag
      961 caagtttgaa aaggcggttg ttatgtagtt ccatttcatt taatacttca tggcacgttt
     1021 cacgcattaa ttttgcacga ggatcaaaat ttttatagac gcgatgaccg aaccccatta
     1081 atcggaatcc gtccgtttta tcttttgcac gtttaatata ttcattgatg cgtgaaacat
     1141 ccccgatctg ttccagcata tttaagcagg cctcattggc gccaccgtga gcgggtcccc
     1201 ataagcaggc gattcctgct gatatacacg cgaatggatt ggcaccactt gatccagcga
     1261 gccggaccgt tgaggtcgat gcattttgct catgatcggc atggagaatc agtattcgat
     1321 ctagtgctct gacaataacc ggattcggtt catatttttc tgctggtgtc gcaaacatca
     1381 tgtgcaaaaa gtttgctgca tagcttaagt cgttgcgtgg gtagataaaa ggttggccaa
     1441 tattgtattt gtaggccatt gcagcaatag tgggcagctt ggcgattaaa cggaaggctg
     1501 attgctcacg atgtagcacg tctgaaatat ccattgcctc atggtaaaaa gcagataaag
     1561 cgcctacgac acctaccata acagccatgg gatgagcatc tcttcggaat cctgtgtaga
     1621 atcgaacgag ttgctcatgc aacatggtgt gagattttat ggtgctcaca aaatgttgtt
     1681 tctgaggatc agtaggtaac tcgccagtca acagtaggtg acatacttcc ataaaatcac
     1741 agcgtgccgc aagttgctca atcggataac ctcgatagag cagtattcct ttatcaccat
     1801 cgataaaggt aatacttgat ttggtgcttg cggtcgatag aaagccagga tcataagtga
     1861 ataggcctgt tttggcatac agtgttcgta tatcaaccgt atcagggccc atattgccgg
     1921 ataaaatcgg aaactcgata ggttcagtgt tctcgccaag tcgtaaggtt gctttacgtt
     1981 gttcagtcat atttattttc tccttctctt tgggttgaga aacgatctcg ttattgattt
     2041 attattaact attctgcact tttacagatt caagaattta acttcgttga atgagctgta
     2101 taagtcgttt taagctttta tcaaccgttt caggatttga acaaattata gctaacagat
     2161 cgttatctga aagtgacaac agtgattcaa attggtctat ttgttgttca tttaattgag
     2221 aatcgtattt attcagaaat cgttgtaaga gtagatcaag ctcgagcaaa cctcttcgac
     2281 agcgccagcg tattcgctcg atacgcttgg tcatactact ttattgagca tcatgccttt
     2341 gatttttcct atcgcattgg ttggattgag tcctttgggg cagacatcaa cacaattcat
     2401 aatggaatga cagcgaaaca ggcggtatgg atcttcaaga taatcaagtc gttcagtggt
     2461 ggcttgatcc cgactatcag caagaaagcg ataagcttgt aagagaccgg caggtcctac
     2521 aaatttatcg ggattccacc aaaaagaagg gcatgaagtt gaacagcagg cgcataaaat
     2581 acattcatat acaccatcta attctgctct ttcgcttggt gactgtaatc gttcagtctc
     2641 ggggggagga tcatggttaa cgagataagg ttttatcgaa tgatactgtt taaagaattg
     2701 ggacatatcg acgatcaggt cacgaataat cggcagaccg ggtaatggac gaatctcgat
     2761 cggttctttg agggtagcga tgggtgtaat gcaagcaagt ccattacgtc cattgatatt
     2821 catggcatct gaaccacata cgccttctcg acaggatcgg cgtaagctca ggctatcatc
     2881 aatagatttg atgcgaatta acgcatctaa aagcatttta tctgtgggtt caagttcaat
     2941 ttcataatcc tgcatatagg gtttttcatc tttatcagga tcaaaacgac taattaagaa
     3001 tcgcatcatt gactaccttt ttagctaaaa cgttaaacta attattcagc atatcagatt
     3061 atgataaatt taaaatgaaa gaaagcgaag atatcggttt tatggaatga atttaaccgt
     3121 tttattatga tcgtacctta cttgaaataa actgctttac ttcattcaca tccttatcca
     3181 ttacgatgaa tcgttgtggg agtttttcaa gatcttcgaa gccaggtatc ggctcaggtt
     3241 ctttcccgat tgcttcttga atactatctg agaatttaac aggtaaagca gtttccaaac
     3301 atatcagcgg tatatctgat tcgcgatttt ctagcccaac tttgatacca tctgcggtat
     3361 gagtatcaac taatacacca tattttttat aagtttctcg aatggtttcg attcgattac
     3421 tgtgatgact tgatcctgag agaaatccta aatcttttgc tttagcccat atggcggttc
     3481 caaataaatc aaagccattt ccattctcaa cagattgcca caattctttg attctagccg
     3541 aatcacgctc agatagatca aatacatagc gctcaaaatt tgatgctttg gagatatcca
     3601 tagaagggct gctagtgtga tgtgtttctg acgtggttcg tggacggtaa agtccagttc
     3661 ggaaaaactc atctaataca tcattttcat tcgttgcaag aatcaaatgc ttgataggaa
     3721 gacccatcat ccgtgcgaca tgtcctgcaa aaatattgcc aaaattacca gatggcacgg
     3781 aaaatgatac gaattcatcg tttgattgcg taacagcgaa atagcctttg aaataataga
     3841 caatttgagc cgcgattctt gcccagttga tcgaattgac agttccaata tgatggccct
     3901 ttttgaaagg gtgatcattg ctgactgctt ttacaatgtc ttggcaatca tcgaataccc
     3961 cttgaattgc aatgttatag atgttttcat cttgtaggga aaacatctgg gcagtttgga
     4021 agcgactcat cttaccataa ggggagagca tgaatacccg tattctttgt tttccacgca
     4081 ttgcatattc tgcgcttgag ccagtatctc ctgaggttgc tcctaggata ttgagttcac
     4141 tgcctgtttt ggcgagagta tattcaaata gattgcctaa gaattgcatt gcaatatctt
     4201 tgaaagcgag ggttggccca tttgataaac ataaaatgtg taatcctggt tccagtgtct
     4261 taatcggtgt gatatcagca ctaccgaata gtttagatgt ataggtacga gtgatgatat
     4321 ctcggagatt ggcggttgga atatcatcca tgaagcggga tagaatttca aaagcaagct
     4381 ccggataact taaatagcgc cacgattcaa gctctgctgc atcgactcga ggataggatt
     4441 cgggtacagc taaaccccca tcttgtgcaa gtccactcaa cagaatttca ctaaaatttt
     4501 gcggcgacat accgcctctg gtagagatat aacgcatagt gtatgaccga tctagaaaag
     4561 agggatggga aaagatgatg ctattttccc aagttaattt atttgtttaa ttcttctaaa
     4621 cgaatacgca cgactttatc tgtcatgcta ggcagttttt caatcttatc gattgctcta
     4681 ttgatattct tttcttgagt agtatgagtt agcataatga tgttaactcg atcttcttcc
     4741 tcagcatgtt ctttttgtat catagcacta atcgagatat ccgcatcggc aaggatacgg
     4801 gtgatatcag ctagcacacc aggcttatca atgacttgaa gtctcaaata gtatgcggtt
     4861 tgtacatctt ccattgagac aatgggggta tcagaaagtg aatcgggagg gaaggccaaa
     4921 tagggaacac ggtgcttggg atctaccgta agttgtcgag caacatcgac caaatcagca
     4981 atcactgaac tggcagtggg ttctgcacca gcgccagcac cataatataa agttgggcct
     5041 accgcatctc ctttaaccac aatggcattc atgacgcctt cgacattagc aatcaaacgt
     5101 ttgataggaa ttaatgtagg atgaactcgt aattcgattc ctttctctac ccgtttggta
     5161 atgcctaata atttgattcg atagcctaat tcttcagcat tacggatatc ttcattattt
     5221 aattgggaga taccttcaat ataagctttt tcaaatcgaa cagggatacc aaacgcaata
     5281 gcagccatga tggtaatttt atgggctgca tcgatccctt caatatcata agtcggatct
     5341 gcttccgcgt aaccaagtcg ttgtgcagtg gcaagtgcag tactaaatgc taatcccttt
     5401 tcacgcattt cggagagaat ataattgctc gttccattga taatacctgc tatccactca
     5461 attcggttag ctgtaaggcc ttcacgcagc gccttgatga ttggaatccc tccagcaacc
     5521 gcagcttcaa aagcgacaat cacccctttt tgctgtgctg ctgaaaaaat ctcactgccg
     5581 tgattagcaa gcaaggcttt gtttgccgtt acgatgtgct tgccattatt aatggcttgt
     5641 aggatgagtt ccttggcaat ggtttgacca ccgataagct caatgataat ttcgatatca
     5701 gggttagcca caatttcttg agcgttttgg gtaacaatcg tttggctacc gacgatatgg
     5761 cgtgcctttt cgatatcttt gtctgcaacc atttgaataa cgatattgca tcctacacgg
     5821 cgcgcgattt cttcttgatt tcgttttaga acagtgaaag taccgccacc gaccgttcca
     5881 attccgagta aaccaacatt aatagatttc atcttatatt cttaagtaaa taagctaatg
     5941 atcaatacgc tctattttaa ttcaacccat ccaggataag ccaatttatt gaatgaatag
     6001 gttgtaatta ggttttatag cgtttccgaa aacgatcgag aaagcgagcg attcgtgaaa
     6061 tagcttcagt caagtcatcg ctattcggta aaaatacaac cctgaagtgg tctggatgta
     6121 accaattgaa acctgtccct tgtacaacta gcactttttc ttctagcaat aaatccaaga
     6181 caaagcgttc atcatcttga attgggtaaa tt
//
LOCUS       DAOQLJ010000131         6067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_5617_length_6067_cov_5.033599, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000131 DAOQLJ010000000
VERSION     DAOQLJ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5617_length_6067_cov_5.033599"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PK522_08985"
     CDS             complement(<1..376)
                     /locus_tag="PK522_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011497995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC41905.1"
                     /translation="MFNSVQHNVIKHKIGLLNLAAELGNVSRACKVMGFSRDTFYRYQ
                     AAVETGGVEALIDANRRKPNIKNRVEEATEAAVTAFALEQPAFGQVRVSNELRKRGIF
                     VSSSGVRSVWLRQNLESFKKRLS"
     gene            complement(551..1006)
                     /locus_tag="PK522_08990"
     CDS             complement(551..1006)
                     /locus_tag="PK522_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC41906.1"
                     /translation="MKAYAVGINHVALEVGNIHEALKFYSDFLSFEVHQENEMQAFIY
                     FGDQFLNFIKNDNRQPDQMRHFGIAVDNKELVRQTLEGMGIKLLNGPFLDFLDPWGNR
                     VEITTYSNIQYTKADHVLLGMGLGHLKKTDQALAELNAKGLGPIQYDNN"
     gene            complement(1142..1921)
                     /locus_tag="PK522_08995"
     CDS             complement(1142..1921)
                     /locus_tag="PK522_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HNC41907.1"
                     /translation="MDQVTESAGRGLARFLTKPVRQSTQVAMISQEKLAAALQPGDVL
                     LVEGNTRISVAIKYLTQSTWSHAALYIGDALPSSMSATTPVLVEADLEEGVRAITLAH
                     YAQMHTRICRPVGLNDEDRRRVVDYAIARIGQQYDLKNIFDLVRYLLPIAPVPTRFRR
                     RLLALGSGDPTRAICSTLIAQAFQSVHYPILPEIQRAWLDDPMCADCYAEIYRIRHHS
                     LFTPRDFDISPFFNIIKPTIENGFDYHSLAWSNAIQEPGKM"
     gene            complement(2002..2724)
                     /locus_tag="PK522_09000"
     CDS             complement(2002..2724)
                     /locus_tag="PK522_09000"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNC41908.1"
                     /translation="MGMGIELASIPLAFLAGIIGVLSPCVWPLVPVIMSSAANSGHFG
                     PLFLALGLATAFAVAGTFLTLLLLNLGLNPDVFRPVAAALLILIAFLLISRRLGEWAS
                     NRLSLFTSRFNTAGGGAAGSVAGQFGIGALLGLVWLPCVGPTLGAAIALASIGQNTSM
                     AFIVMFAFGTGTAAALLSTALLSINLLRRIIKPNLMKQADNGKRVLGVLLLALGLIVF
                     TGFDKTLETAALNMLPGWALSI"
     gene            complement(2724..3125)
                     /locus_tag="PK522_09005"
     CDS             complement(2724..3125)
                     /locus_tag="PK522_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNC41909.1"
                     /translation="MKPLFIALMSIVITLSSAAQAAVEKEAFTEARYNELRRSGKVFL
                     VDIYATWCPTCAKQQDILATYGENNPQQNLNVLVVDFDNDKKWVRYFKAPRQSTLLLY
                     SGDEQLWFSVGETRYDVIAGELDKALNTWGI"
     gene            complement(3245..3988)
                     /locus_tag="PK522_09010"
     CDS             complement(3245..3988)
                     /locus_tag="PK522_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41910.1"
                     /translation="MNDKAFEIDHDVFTELSRMQLERMADAGAQIIECYRVLQKGGLN
                     IVGEILKGQGEFYELEHYPKDDVFDGETYSQYYYHAHRAGTNENGHFHTFLRQPGMPA
                     GISPVPYSGAEPWPQGSDALSHLIGISMDAYGFPLGLFATNRWVTAEAWYKAEDVIVI
                     LDYFKVDHAYPSWPVNLWISTMLVLFRPQIEALIRQRDQVIAGWECRYPGVDVYEDRE
                     LELTGYTSINVEQQIRRVVTAIEKRGMTA"
     gene            complement(4044..4721)
                     /locus_tag="PK522_09015"
     CDS             complement(4044..4721)
                     /locus_tag="PK522_09015"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNC41911.1"
                     /translation="MKTKIQPLRATVISALFGAVIGLPLALSGCAMATCKPCAPRAGK
                     RNPCNPCAARNPCTAMNPCAAKNPCAAGDRVDPQAVTRPEGTKPYQGDLAALLNEGKA
                     LWNSKAISRNNSMSCSSCHQDGTAMLQPDFAKPYPHFVQMVADNNGMKSITLEGMIQF
                     CMIKPMQSKPFAWDSRQLAALTAYTAVLQKEFKPVGNAFNPCAARNPCAPKNPCSMRN
                     PCAVRSY"
     gene            complement(4816..5067)
                     /locus_tag="PK522_09020"
     CDS             complement(4816..5067)
                     /locus_tag="PK522_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41912.1"
                     /translation="MKTRQKLLTSSMLAGLMAISMSAPTFAESNPCAPKAKAQHQHDM
                     KNPCAGKNPCAAKNPCAGKNPCATKNPCAAKNPCAAKKY"
     gene            5584..>6067
                     /locus_tag="PK522_09025"
     CDS             5584..>6067
                     /locus_tag="PK522_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HNC41913.1"
                     /translation="MAELPTLVLICKRPMLGFGKQRLAAKLGMEVTHRVAEALLACAL
                     EDAYNWPGPIVIAPASPDDHDWAVAVSSPARSPVIVLPQVSGNLGQRLNVLDSALRSK
                     GMKQLVFIGSDSPGLAQTDYVAVINALQCDDTVLKPALDGGVVLMASNCPWPELTDLP
                     W"
BASE COUNT         1597 a         1408 c         1363 g         1699 t
ORIGIN      
        1 ctgacagacg cttcttgaac gactccagat tttggcgcag ccacactgaa cgaacgccgg
       61 atgaagaaac gaagattccg cgtttgcgta gttcattgga aacacgaact tggccaaaag
      121 ccggttgctc cagagcaaaa gcggtgaccg ccgcctctgt cgcttcttct acacggttct
      181 taatgttggg cttgcgccgg tttgcatcaa tcagtgcctc aacaccacct gtttccaccg
      241 ctgcctgata gcgatagaaa gtatcgcgag aaaaacccat caccttgcaa gcccgggata
      301 cattgccaag ctcggctgcc agattaagca gaccaatctt gtgtttgatg acattatgtt
      361 gaacactgtt gaacactact catgggatta ctcctttacg ctttcgcgtt tggttgataa
      421 agattcgcac ctctatcaaa ccggtaatcc caccctttgg caagacctta ttgtcagatc
      481 aaatctgaac tactacaaat tattaccatt taattattac tgtttctatc tgtaattgga
      541 attaggtcaa ttaattatta tcatattgaa taggtcctaa accttttgca ttcaattctg
      601 ccagtgcttg atcagttttt ttaagatggc ccaatcccat tcccagcaat acatggtcag
      661 cttttgtata ttgaatattg ctataggtgg ttatttctac ccgatttccc caaggatcaa
      721 gaaaatcaag aaaagggcca tttaaaagtt tgatacccat cccctcaagt gtctgcctca
      781 ctaattcttt attgtctacc gctattccaa agtgtcgcat ttgatcaggt tgtctattat
      841 cattttttat aaaattgaga aattgatccc caaaataaat aaatgcttgc atctcatttt
      901 cttgatgaac ttcaaagctc aagaaatcac tataaaattt taacgcttcg tgaatatttc
      961 caacttctaa tgccacatga tttatgccta ctgcatatgc tttcatattt tttcctttgt
     1021 cattgaaatt caatctaacc caaataatct attcttgtgc tattgaagat aatattgaag
     1081 aaagttaatt aagcaattaa tgcataaata gctaatctgc attagtcttc attgatttgt
     1141 atcacatctt ccctggctct tgtatggcgt tactccaggc aagcgaatgg tagtcaaagc
     1201 cattttcgat ggttggtttg ataatgttga agaagggaga aatgtcgaaa tcccgaggag
     1261 tgaacaaact atgatggcgg attcgataga tttccgcata acaatcagcg cacatcggat
     1321 cgtctaacca ggcacgctga atctccggca agatcggata gtgcaccgac tgaaacgctt
     1381 gcgcaatcaa ggtcgaacag attgcacgag ttggatctcc gctaccgagg gccaacaggc
     1441 gtctgcggaa tcttgttgga acgggtgcta tgggaagaag atagcgtacg agatcgaaaa
     1501 tgttcttgag atcatactgt tgaccgatac gtgcaatggc ataatccaca acgcgcctac
     1561 gatcttcatc attcaaacca acagggcggc agattcgggt gtgcatttgc gcataatgag
     1621 caagcgttat tgcacgcacg ccttcctcca ggtccgcttc aaccagtacc ggcgtagttg
     1681 ccgacatgct ggaaggaagt gcatcgccga tatacagtgc tgcatgtgac caggtcgatt
     1741 gcgtaagata tttgatggca acactaatac gggtatttcc ttcaaccaat aaaacatctc
     1801 cgggttgtaa agctgccgcg agtttttcct ggctgatcat ggcgacctga gtgctttgcc
     1861 ggactggctt ggttaaaaat cgtgccagac cacggcctgc actttcagtt acctgatcca
     1921 caaattcctc tttttatcgc tagttccgga tttttgttca ctataatttt tatcatccta
     1981 taacaacgaa tcgttttcct gtcaaataga cagcgcccaa ccggggagca tgttcagtgc
     2041 ggctgtctcc agcgtcttgt caaagccggt gaagactatt aaacctagcg ccagcagcag
     2101 aacacccagg acacgtttgc cgttatccgc ctgtttcatt aaattcggct tgatgattcg
     2161 tctgagcaga ttgatggaaa gtaatgctgt gcttaatagc gccgcagcgg tgccggtgcc
     2221 aaaggcaaac atgactatga atgccatgct tgtattctgt cctatggaag ccaaggcaat
     2281 ggcggctcct aaagttggcc cgacgcaggg cagccagacc agacccagaa gtgcgccaat
     2341 tccgaactga ccggccacag agccggccgc gccaccaccc gcagtattga aacggctggt
     2401 gaagagcgac agccggttgc ttgcccattc tcccagccgt ctggatatca atagaaatgc
     2461 gatcagtatc agcagcgcgg ccgcgacagg ccggaacaca tccggattca atccgagatt
     2521 gagcaacagc agtgtgagga atgtgcccgc aacagcgaaa gcagtggcca ggccaagtgc
     2581 cagaaataat ggtccgaagt gaccgctgtt tgccgccgat gacatgatta caggaaccag
     2641 cggccatacg caaggagaaa ggacgccaat aataccagcc agaaaggcca gagggatcga
     2701 ggccagttca attcccattc ccatcagatg ccccaagtat tcagagcctt atccaactcg
     2761 ccggcaataa catcgtagcg tgtctcgccc acgctgaacc agagttgttc atcaccggaa
     2821 tacaacaaca aggttgattg tctgggtgcc ttgaaataac gcacccactt cttgtcatta
     2881 tcaaaatcca ccaccaggac attcagattt tgttgaggat tgttttcacc gtaggttgcc
     2941 agaatatcct gttgcttggc acacgtagga caccaggttg cataaatatc caccagaaaa
     3001 accttaccgg atctccgaag ttcattgtaa cgggcttcag taaaagcctc cttttcaacg
     3061 gcagcttgtg cggcagagga tagcgtgatg actatgctca tcagagcaat aaaaagtggt
     3121 ttcataattt atggtgtctt ttgaaattaa atttgtttgt cattttttgt tctgctgcac
     3181 cgggtaaggt taacttgatc tggcgtgaat aatatttctt cagactttat gtaaaacaaa
     3241 gacatcatgc tgtcatgccg cgcttttcaa tggctgtcac aacacgcctg atctgttgct
     3301 caacattgat agatgtgtaa cctgtgagct caagttcccg gtcctcatag acatcaacac
     3361 ccggatatct acactcccaa ccagcaatca cttggtcacg ttgccgtatg agcgcttcta
     3421 tctgtggccg gaataaaacc agcatggtgg atatccatag gttaaccggc cacgaaggat
     3481 aggcatggtc taccttaaaa taatccagta tgacaatgac gtcctcggct ttgtaccagg
     3541 cttctgctgt tacccatcga ttggttgcga ataatccgag aggaaagccg taggcatcca
     3601 ttgaaatgcc aatgagatgg gaaagcgcgt cacttccctg tggccaaggc tctgctccac
     3661 tgtagggaac cggtgatatt ccggctggca ttcccggctg acgcaagaat gtatggaaat
     3721 gcccgttctc attagttccg gcacggtgcg cgtgatagta gtactgactg tatgtttccc
     3781 catcaaacac atcatccttg ggatagtgtt ccagttcgta aaactcgcct tgtcctttta
     3841 gtatctctcc aacgatatta agtccgcctt tttgcaatac ccggtagcat tcgataatct
     3901 gtgcaccggc gtcagccatt cgttcaagct gcatgcggga taattcagtg aaaacgtcat
     3961 gatctatttc aaatgccttg tcattcataa aaaagcgatg aagcctgtat caacacctca
     4021 tcacaacctc cctgacacgt taattaatat gacctgacag cacatggatt tctcatgctg
     4081 catgggtttt ttggcgcaca aggattcctg gcggcacatg ggttaaacgc gttacctact
     4141 ggcttgaact ctttctgtaa taccgctgta tatgctgtta atgcggccaa ttgacgggaa
     4201 tcccatgcaa agggtttact ttgcatgggt ttgatcatgc aaaactgtat catcccttcc
     4261 agcgtgattg atttcatacc gttgttgtcc gctaccattt gcacaaagtg tggataaggt
     4321 ttggcaaaat cgggttgcag catcgccgtg ccatcctgat gacagcttga acaagacatg
     4381 gaattattac ggctgattgc tttgctgttc cacagcgctt tgccttcatt taataaagcc
     4441 gccaggtctc cttgataggg ttttgttccc tcgggtcggg ttaccgcctg aggatccact
     4501 ctatcacctg cagcacaagg atttttagcg gcgcatggat tcatggctgt acagggattt
     4561 cttgccgcgc aagggttgca tggattacgt ttgccagcac gcggcgcaca cggtttacag
     4621 gttgccatgg cacagccact taatgctaaa ggcaagccaa ttactgcacc aaacaaagca
     4681 gaaataacgg ttgctcttaa gggttgaatt ttagttttca taattttcct tacgttacag
     4741 cgaagttctt atgttgcggt taatgcatta aacaaagcac cccactgcaa gagcggggaa
     4801 ttgaactcgc agagattaat atttttttgc tgcgcaagga tttttagcgg cacagggatt
     4861 cttggtcgcg caaggatttt taccagcgca gggattctta gccgcacaag gattcttacc
     4921 agcgcaggga tttttcatgt catgttgatg ttgtgctttc gcttttggtg cacacggatt
     4981 actctcagca aatgttggcg cgctcatgga gattgccatc aagccagcta gcatggaaga
     5041 ggtcaaaagt ttttgacgtg ttttcattat taattctcct aagattaagt tacggggtga
     5101 agcaaccagc catcattggc ggttcgaaac aagaactcgg cataaaatat ccagattctt
     5161 gcaacttagt cgaaggtaat tggatttcct tacaatactg tttatcttat caagtttaac
     5221 gggaactcca ttaactgtgt agttctacgg tttgctggtt tggatagccc tttaataatt
     5281 cacctcaata ggttgctgaa aacaataccc ccttccttct ttccgtccat gtgttaattt
     5341 ttggcaatct gttaaatatt cctttagtag cagtaggtag tgctgtaatg gggtatgttt
     5401 ttgtgcagtt ggttttaact tcatatcagt tcgattatta atagatatct atgatgtaag
     5461 gaatcccgct cgctatcgac taataattta gttacagaag aaaagatgct cattttaggc
     5521 tgcaagaagt ctttagcttc ttgataactc gattaattta tttactaagc aagaagaatc
     5581 aatatggctg aactccccac tttagttttg atatgcaagc gaccgatgct gggtttcggc
     5641 aaacagagac tggctgccaa actgggtatg gaagtaacac accgtgttgc cgaagcgttg
     5701 cttgcctgtg cgctggaaga cgcctacaac tggcctggcc caattgtgat tgctccggca
     5761 agcccggatg atcatgattg ggcagttgct gtatcttcac cggcgagatc acccgtgata
     5821 gttctaccac aggtatccgg taatctgggg caaagactaa acgtgttgga ttctgcatta
     5881 cgtagcaagg gaatgaaaca attggtgttt atcggcagtg attcaccagg gcttgctcaa
     5941 actgattatg tggcggttat aaacgcttta cagtgcgatg atacggtgct taagcctgca
     6001 cttgatggtg gtgttgtact gatggcaagt aattgtccgt ggccggaact aactgatctg
     6061 ccgtgga
//
LOCUS       DAOQLJ010000132         5983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_5743_length_5983_cov_6.705297, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000132 DAOQLJ010000000
VERSION     DAOQLJ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5743_length_5983_cov_6.705297"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /gene="thpR"
                     /locus_tag="PK522_09030"
     CDS             complement(<1..499)
                     /gene="thpR"
                     /locus_tag="PK522_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNC41914.1"
                     /translation="MIELQSERIPIKVFFAIWPDLHVKTQLSRLTGNLEHLCGGYRTN
                     SLKLHLTLVFLGNILMKQLAYLQEIASQIKLPCFSLTIDTIEYWKHNKIVYAGITDTP
                     VELIKLVSQLHHDLQLAGFKLEQRSYIPHLTLIRNATCQQLPKLTTPIHWNIKEWMLI
                     QSTPTK"
     gene            complement(503..997)
                     /gene="ispF"
                     /locus_tag="PK522_09035"
     CDS             complement(503..997)
                     /gene="ispF"
                     /locus_tag="PK522_09035"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNC41915.1"
                     /translation="MKNLRIGQGFDVHQLIEGRKLIIGGVTIPYEKGLLGHSDADVLL
                     HALCDALIGAAALGDIGKHFSDKDNRYKDIDSRLLLREVHQLIKQKHYDVINIDATII
                     VQAPKMAPYILAMIANIAEDLKMPSSDINIKAKTAEKLGFIGRGEGIVAEVVCLIAKT
                     DELD"
     gene            complement(994..1698)
                     /gene="ispD"
                     /locus_tag="PK522_09040"
     CDS             complement(994..1698)
                     /gene="ispD"
                     /locus_tag="PK522_09040"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNC41916.1"
                     /translation="MSQFFALIPAAGFGSRMGHQLPKQYLPLVEKPMIYHALHTLVNC
                     SKITRVFVILAPDDDIWVNYNWTELQDKLVVEYCGGDARAESVLNGLNAMQSSGMVNR
                     DDWVLVHDAARPCLSAPQLDQLINELSYDGVGGLLAIPVADTLKRADADHRIISTESR
                     ERLWQAQTPQMFRFNLLKDALSQVDSHQITDDASAIEALGLKPKLVLSDAYNFKVTYP
                     QDLALAELILQKREAK"
     gene            complement(1821..3158)
                     /locus_tag="PK522_09045"
     CDS             complement(1821..3158)
                     /locus_tag="PK522_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC41917.1"
                     /translation="MASLNQRVLVSASLVLILFLAGTAVTLEQAFHDSSKKALQDRML
                     GKLYLLMGEAEVNESGLFQMPAEVPGLIEFEQLNSGIYAYVSDQSDRVIWESKSSLSI
                     ELPIPKHLAEGDEVFEPIMLAHVPHFLYSYGVAWETESGGHYPFTFHVIFDSTSFEEQ
                     IDRYRSDLWGLFAVMAIVLLLTQMMVLRWGLKPMRQVSAELNAIEAGLQDKIQGDYPI
                     ELKRLTDNINSFVYRERKHQQRYRNALADLAHSLKTPLTIAKGAISTEHDSQTLIDTI
                     GEQLDRMNNIVHYQLQRASTAGVSSRMSYLELRPIIERLIHTIERAHLEKHPNVSYVI
                     SPDIRLRMDEGDLMELLGNLIDNAYKWCHRAITIKAKQESNHTIISIENDGPGIQPEE
                     VDKILERGVRADQSIPGHGIGLAIVRDIVQAYDGTLSIQSNGDTGVSFIIKLK"
     gene            complement(3158..3841)
                     /locus_tag="PK522_09050"
     CDS             complement(3158..3841)
                     /locus_tag="PK522_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC41918.1"
                     /translation="MRILIIEDEIKLQRQIQLQLEAQGFVVDTCSNGKEGLYFASEYP
                     LDAAIIDIGLPDLSGLDVIAALRKQGSLLPILILTARNSWQEKVKGLEMGADDYLTKP
                     FQMEELLARLKALLRRATAVPDTEIRSGAILLDMEAQTVKLNSQLVDLTAFEYRLLEH
                     LMRHQGEVISKQELTDYLYPHNDDHDSNVLEVMVGRLRRKLDPDGSLNPIETLRGRGY
                     RFIAIAQAS"
     gene            complement(3884..4162)
                     /locus_tag="PK522_09055"
     CDS             complement(3884..4162)
                     /locus_tag="PK522_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNC41919.1"
                     /translation="MKFQSKVLVVMLWVALVSGESFAQMKLASELSDEQIEISQQQAV
                     TIAQRYTHGRLLSVQRMDDHYRIKILNHKGAVHIVTINAKNGEVDLSQ"
     gene            complement(4227..4580)
                     /locus_tag="PK522_09060"
     CDS             complement(4227..4580)
                     /locus_tag="PK522_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41920.1"
                     /translation="MNTQFNRAKITRFVTIALLSVALIVPIAAEAGRGHHRHHHHHKH
                     HHHDHHLHGHSGYGGYGYYGKPRYNNYRYVERHYYSQPYTYYAPAYSGYYYEGVPGNF
                     YLGINAGNGNFMLGY"
     gene            complement(4716..5042)
                     /locus_tag="PK522_09065"
     CDS             complement(4716..5042)
                     /locus_tag="PK522_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41921.1"
                     /translation="MKKHFVITTLVFFLIHLFFANVYAESPVRKSVETRKVDLDHDRE
                     NNMRNTEMKQHQKSKSLQKAKNEEKQNSSHSDESKHRQELEQSYKKQWEEVEKGSGDP
                     RLVIGR"
     gene            complement(5166..>5983)
                     /locus_tag="PK522_09070"
     CDS             complement(5166..>5983)
                     /locus_tag="PK522_09070"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNC41922.1"
                     /translation="AGLNIQYLQAKLSNAVDFGAICLQAIGLGACGSRGLLPQSADGM
                     VTLKGDSVGFGFNLGLYFEPSQITRLGISYRSRVEHEVKGTANYAVPDQALILTRGGQ
                     FIDTHTQTRVIMPDQVMLGFSQYLGARWNLTGDALWTNWSLNRELRTDFASPQGDSIQ
                     QLNWNDTWRLALGLSYTPEVQKWTIRTGFAFDQTPVPNADFRPARIPDNDRYWLTAGF
                     SYRVLSNLTLHGAYAHLFIPDPAINSKSATGDRLSGQYTAQVNIVGLQLDWRF"
BASE COUNT         1779 a         1340 c         1085 g         1779 t
ORIGIN      
        1 ttttcgttgg cgttgattgt attaacatcc actccttgat attccagtga atcggtgtcg
       61 tcaatttagg aagttgctga caagttgcat ttctaattaa cgttaaatgc ggaatatatg
      121 agcgttgttc caatttgaat ccagccaact gtaaatcatg atgcagttga ctaactaatt
      181 ttattaactc aacaggtgta tcggtgatac cggcataaac aattttatta tgtttccaat
      241 attcgatcgt atcaatggtc aaactgaaac aaggtagctt aatttggctt gcgatctcct
      301 gaagataagc gagttgcttc atcaaaatat tgccaagaaa aaccagcgtt aagtgcaatt
      361 taagcgaatt cgttcgataa cccccacaaa gatgctcaag atttcccgtt agtcggctca
      421 attgcgtttt tacgtgtaaa tctggccaaa ttgcaaaaaa aaccttaatc ggtattcttt
      481 ccgattgcaa ctcaatcacg tgctaatcaa gttcatcagt tttagcaatc aagcaaacga
      541 cttctgcaac aatgccctct ccacgaccaa taaaaccaag cttctcggct gttttggctt
      601 tgatattaat atcactggaa ggcattttta gatcttcagc aatgttagca atcatcgcaa
      661 gaatataagg ggccatttta ggagcctgga cgataatggt tgcatcgata ttgatgacat
      721 catagtgttt ctgtttgatt aattgatgta cttcacggag taataagcgg ctatcgatat
      781 ctttgtagcg attatcttta tcagaaaaat gcttgccaat atctcccaaa gcagcagccc
      841 caatcaatgc atcgcataag gcatgtagta gcacgtctgc atccgaatgc cctaacaaac
      901 ctttttcata aggaatcgta acgccaccaa tgatgagttt tctaccctct atcaactgat
      961 gcacatcaaa accttgtcca attcgtagat tcttcatttc gcttcccttt tttgcagaat
     1021 cagttctgct aaggctaaat cttggggata agtaacttta aaattatacg catcactcaa
     1081 tacgagttta ggcttcaacc caagcgcttc gattgcactc gcatcatcgg taatttgatg
     1141 gctatccact tgacttaagg cgtctttcag tagattaaaa cgaaacattt ggggagtttg
     1201 tgcttgccat aatcgttctc ttgactcggt cgatataatg cgatgatcag catctgcacg
     1261 ttttagggta tctgcaactg gaattgctaa taaaccacca accccgtcat aagataactc
     1321 attgatcaat tgatcgagct gtggcgcact taaacaaggc cgcgctgcat catgaaccag
     1381 tacccaatca tctctattta ccatcccact tgattgcatc gcgttcaatc cattaagcac
     1441 actttcagca cgcgcatccc ccccgcaata ctcaactact agtttgtctt ggagctctgt
     1501 ccagttgtaa tttacccaaa tatcgtcatc tggcgcaaga atcacaaaaa cacgcgtaat
     1561 tttggaacaa ttgactagcg tatgtaacgc atgataaatc attggttttt caaccaaagg
     1621 aagatattgt ttaggcaact ggtgccccat tcttgaacca aaacctgctg ccggaatcaa
     1681 cgcaaaaaat tgagacatat ggtttattta aatcgctgag tagttttcac taaccaataa
     1741 ttatcgcaca ccctagatag ctatccacgt tcgttagcag aaaattattc aacgcctcaa
     1801 ctttctgaga ttacgattaa tcacttgagc ttaataatga aagatacgcc tgtatctcca
     1861 tttgattgaa tagataacgt tccatcatag gcttgcacga tgtcacgtac aatggcaagg
     1921 ccaattccat gccctgggat tgattgatct gctctaaccc cccgctctaa aattttatca
     1981 acttcttcag gttggatacc aggcccatcg ttttcaatgc tgatgatcgt atgattgctt
     2041 tcctgtttcg ctttgatcgt aatagcccgg tgacaccatt tataagcatt gtcaatgaga
     2101 ttacccaata actccatcaa gtctccttca tccattctga gacgaatatc agggctaatc
     2161 acataactca cgttgggatg tttttctaaa tgggctcgct caatggtatg aatgagcctc
     2221 tcgatgatag gccgtaattc aagataactc atccttgaag aaaccccagc agtggaagct
     2281 cgctgtaatt gataatgaac aatattgttc attcgatcga gttgctcacc gatggtatct
     2341 atcaaagttt gtgaatcatg ctctgtacta attgctcctt ttgcaatggt aagtggcgtt
     2401 ttaagactgt gcgccaaatc tgccaacgca ttacgatagc gctgctgatg tttacgttct
     2461 cgataaacaa aagaattaat attatccgtc aaacgtttaa gttcaatcgg ataatcccct
     2521 tgaattttat cttgcaggcc tgcttcaatt gcattcaatt cagccgatac ttgccgcatc
     2581 ggtttaagtc cccaacgtaa taccatcatc tgcgtaagca gcagtacaat agccatcaca
     2641 gcaaataacc cccatagatc actccgatat cgatcaattt gctcttcaaa agaagtgcta
     2701 tcaaaaatga catggaaggt aaatggataa tgtccaccac tttcagtttc ccacgctaca
     2761 ccgtagctat aaagaaaatg ggggacatgc gccagcataa ttggttcgaa cacttcatca
     2821 ccttctgcaa ggtgtttagg tataggcagt tcaatactaa gcgatgattt agattcccaa
     2881 atgactcgat ctgattgatc tgaaacataa gcataaatcc ctgaattcaa ttgctcaaat
     2941 tcaattaatc caggcacttc tgcaggcatt tgaaacaatc cagattcgtt aacctcggct
     3001 tcccccatca ataaatagag tttacctaac attctatcct gcaaagcttt cttactacta
     3061 tcatgaaacg cttgttccag ggtaactgct gttcctgcta aaaataaaat aagaactagg
     3121 gatgcactaa caagaacgcg ttgattcagt gaggccatta ggaagcttgg gcaatggcaa
     3181 taaaacgata acctctccct cgtaaggttt caatcggatt gagtgaacca tcgggatcaa
     3241 gcttgcgcct tagccgtcca accatgactt ccaagacatt gctatcatga tcatcattat
     3301 gcggataaag ataatcagtc aattcttgtt ttgaaatcac ttcgccctga tgacgcatca
     3361 gatgttctaa aagccgatac tcaaaggcag tcaaatcaac caattgacta tttagtttta
     3421 cagtttgtgc ttccatatcg agcaagatag caccacttct gatttctgtg tctggaactg
     3481 ccgttgcacg ccttaacaaa gccttgagtc tagctaatag ctcttccatc tgaaaaggct
     3541 tggttaaata atcatctgcc cccatttcca gacccttaac tttttcttgc caactatttc
     3601 gtgcagttaa gattaatatc ggtaataagc taccctgttt ccgcaaagca gcaatgacat
     3661 ctaatccaga tagatcaggc aagccaatat caataatggc agcatccaga ggatattcac
     3721 ttgcaaaata taaaccttct ttgccattgc tgcacgtatc aaccacaaat ccctgcgctt
     3781 caagctggag ttgtatttgt ctctgtaatt taatttcatc ttcaataatc aaaatacgca
     3841 tgataggtta ggcaatagta acaattaaaa gctgaatgct attttattgg gataaatcaa
     3901 cttcaccatt tttagcatta atggtcacaa tatgaacagc ccctttatga ttgaggatct
     3961 taatccggta gtgatcatcc attcgttgca cagaaagtaa acggccatgg gtataacgtt
     4021 gagcaatagt gacggcttgc tgttgcgata tttctatttg ttcatcgctc aactcactcg
     4081 ccagcttcat ctgggcaaaa ctttctcctg ataccaaagc cacccataac atcacaacca
     4141 ataccttgct ttgaaatttc atcacacacc gtgtaaatca agataaatgt tcactgatgg
     4201 aatcatcaaa cgttaagata cttatctcaa tagcctagca taaaatttcc atttcctgca
     4261 ttgatcccta gataaaaatt acccggaacg ccttcatagt aataaccgct ataggcagga
     4321 gcataatagg tataaggttg gctatagtaa tggcgctcaa catagcgata gttattgtaa
     4381 cgaggtttac cataatagcc ataaccgcca tagccactat gaccatgtaa atgatgatca
     4441 tgatggtgat gtttatgatg gtggtggtga cgatgatgac ctcttcctgc ttcagcagcg
     4501 atggggacaa ttagcgctac gctcagtaac gcgatcgtaa cgaaacgagt tattttagct
     4561 cgattaaatt gcgtgttcat gtgatctctc ctctaaagtt tgttcattga gcagaataat
     4621 ttctgcaatg aacatcactc tacagggaga tacatgaaca aaaactgaat cgtttgataa
     4681 atgatggaat gattacaact tcgcgtaaaa cagatttacc gtccaataac aagacgaggg
     4741 tcgccgcttc ctttctcaac ttcttcccat tgcttcttat aagattgctc taactcctgt
     4801 cgatgtttgc tttcatcaga atgcgatgaa ttctgttttt cttcattttt tgctttttga
     4861 aggcttttac ttttttgatg ctgtttcatt tcagtatttc tcatattatt ttcacgatcg
     4921 tgatcaagat caacttttct ggtttcaacg ctttttctta ctggtgattc agcataaaca
     4981 tttgcgaaaa acaaatggat gagaaaaaaa actaaggttg taattacaaa atgctttttc
     5041 atgattgaat tccttctatt gattgaaatg aggttagaaa taaacacttt gcagtgtaaa
     5101 acacacttca ctcgatcaaa ttgcgaataa aaaggaattt tgccaaacgt gcatcaagat
     5161 aaccgttaga aacgccaatc taactgtagc ccgacgatat taacttgtgc cgtatattgc
     5221 ccacttagac gatctccagt tgcactttta ctatttattg ccggatctgg gatgaacaga
     5281 tgcgcataag cgccatgtag ggttagattg gacaatacac gataactaaa acctgccgtt
     5341 aaccaatacc gatcattatc aggaatgcgt gcaggtctaa aatcagcatt gggtacgggt
     5401 gtttggtcaa aagcaaaacc tgttctaatc gtccacttct gtacctcagg tgtataactc
     5461 aaccctaaag ccaatcgcca agtgtcattc cagttgagtt gctgtataga gtccccttgg
     5521 ggtgaagcaa aatcagtccg taattcacga ttgagactcc aattggtcca cagtgcatca
     5581 cccgtcaaat tccaccgagc accgagatat tgtgaaaaac ccaacatcac ctggtccggc
     5641 ataataacgc gcgtctgagt atgcgtatcg ataaactgcc ctccccgcgt tagaattaag
     5701 gcttgatcag gtaccgcata atttgcagtg cctttaactt cgtgttcgac tctcgatcga
     5761 taactgatcc caaggcgggt gatttgactc ggttcaaagt agagcccgag attaaaacca
     5821 aacccgacac tatctccctt taaagttacc attccatcag ctgattgggg taataatcct
     5881 cggctgccgc aagcgccaag accaattgct tgcaagcaaa ttgccccgaa atcgaccgca
     5941 ttggataatt ttgcttgtaa atattgaata tttaaccctg cgc
//
LOCUS       DAOQLJ010000133         5978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_5752_length_5978_cov_6.274861, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000133 DAOQLJ010000000
VERSION     DAOQLJ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5752_length_5978_cov_6.274861"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..1150
                     /locus_tag="PK522_09075"
     CDS             11..1150
                     /locus_tag="PK522_09075"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HNC41923.1"
                     /translation="MHIGIPAEIFAGETRVAATPETVKKYTAKGLHTVLVQTGAGAGA
                     SIPDNAYQEAGAVIVSDAAQVYSQAQIVLKVRGPTADELPMLRKDLVLIGLLSPHHTD
                     GISTLAGRGVTAFAMEKLPRISRAQSMDVLSSQANIGGYKAVIIAADTYQKFFPMLMT
                     AAGTVKAARVLVLGAGVAGLQAIATAKRLGAVIEAFDVRPAVKEQVESLGAKFVEVPL
                     TDEEKAKAKAKAETAGGYATEMSDDYKRRQGELINERAAAADIIITTALIPGRPAPVL
                     IKEETVKAMKPGSVIVDMAVEAGGNCPLSELNKTVTKYGVHLIGVANLPGLVAADASA
                     LYARNLMNFLNLMLDAKTGELNINLEDEIIAGTMVCSGGDLAKKA"
     gene            1197..1505
                     /locus_tag="PK522_09080"
     CDS             1197..1505
                     /locus_tag="PK522_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HNC41924.1"
                     /translation="MIGDIDPTIIHLTVFVLAVFVGYHVVWNVTPALHTPLMSVTNAI
                     SSIILVGAMLAAGPLEADWGVWLGAIAVVLASINVFGGFLVTQRMLEMFRKKDKKGNA
                     "
     gene            1506..2885
                     /locus_tag="PK522_09085"
     CDS             1506..2885
                     /locus_tag="PK522_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNC41925.1"
                     /translation="MSANFIALAYLVAAVFFILALKGLSSPLSSRRGNLFGMVGMAIA
                     VATTLTITQNWMLILGCIAIGGIIGSIVANRVQMTAMPELVAFMHSLVGLAAVFIAIA
                     AVNNPVSFGLPETLPMGSKLELFLGTFIGAMTWSGSVIAFLKLSGKMSGAPVIFSGQH
                     AINLLLAIVMIGFGLWFFFSETTNWTAFIAMTAIAFVLGFLIIIPIGGADMPVVISML
                     NSYSGWAAAGIGFSLGNPMLIIAGSLVGSSGAILSYIMCKAMNRPFLSVILGGFGGDA
                     GSQAASDGTQKTYRSGSAEDAAFLMSNADSVIIIPGYGLAVARAQHSVKELAEALHKK
                     GVNARFAIHPVAGRMPGHMNVLLAEAEIPYEQVQEMEEINSDFSTTDVVLVLGANDVV
                     NPAANVPGSPIYGMPILEAHKARTVIVVKRSMAAGYAGLDNDLFYMDKTMMVFGDAKK
                     VVEDMVKSI"
     gene            complement(2928..3617)
                     /locus_tag="PK522_09090"
     CDS             complement(2928..3617)
                     /locus_tag="PK522_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC41926.1"
                     /translation="MRCRDCKARFWVKNRDAYIVASVVFICSLLLITFIWIIISLDEI
                     SIDGKKFDKQLASNQSTANQSSNSFPTTYPKAAIEATNTQQNGVVNQSMPAETQYSSD
                     DAHHHMINLFLNRANEGNAEAQHQLGQLYLTGNGTLQDFEEAVKWFSLAAEQNHAPAQ
                     YELGLLYHIGQGVNIDNEKSYMWLNLSTAGGYEQAILARDKVARSLNPAQLVQAQKAA
                     KEWLNTRINKK"
     gene            complement(3831..4607)
                     /locus_tag="PK522_09095"
     CDS             complement(3831..4607)
                     /locus_tag="PK522_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNC41927.1"
                     /translation="MPLSAVLFDVDGTLADTERDGHRLAFNSAFKELGLNWNWDIELY
                     GKLLQITGGKERIRYYIDHYSPEISKNTNLTDWVAELHKTKTKYFLQLLSEGKIPLRP
                     GVARLIQELRNNAIKIAIATTTTPENVYALLKSTLGEDSPSWFDVIGAGDIVPHKKPA
                     PDIYHWVLDRLDLTPAECIAIEDSENGLRASLTANITTIVTTSEYTHSHDFKGAAVVL
                     TDLGEPNKPATILANSFFPEPKSASIDISFLETVMNNQCK"
     gene            complement(4629..5666)
                     /locus_tag="PK522_09100"
     CDS             complement(4629..5666)
                     /locus_tag="PK522_09100"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNC41928.1"
                     /translation="MAITTSIAKTDLLKEQIFKAARLLQTGNNIVFPTETVYGLGADI
                     THLSAVNRIFQIKERPTNHPLIVHFADLGQISHWAENIPSQAKKLIEYFWPGPLTIIL
                     PKSRHVPYCVTGGQDTVGLRIPDHPVAIALLKALGPQKAVVAPSANRFGCLSPTAALH
                     ISKEISAEVSMILDGGPCKIGLESTIISFCDLKPVLLRPGGIPLNAIEEILNQNIIVS
                     VNHSNNLRASGTLASHYAPRTPLEVWPTETLQHRVDELLNQKYHVAILSWSEAFYSQH
                     QTTEKIIKSLKHHRMPSDPFDYGYHLYAKLHHLDDEGYDRLLVEMPPNLPDWLAVHDR
                     LQRASHHFAIR"
BASE COUNT         1651 a         1084 c         1418 g         1825 t
ORIGIN      
        1 gagataatca atgcacatag gcatacctgc agaaattttt gcgggagaaa cgcgggtcgc
       61 agctacgcca gaaacagtaa aaaaatatac tgctaagggg ttgcataccg tattggtcca
      121 aactggcgca ggagcgggtg ctagtattcc agataatgct taccaagagg caggcgcagt
      181 gattgttagt gatgctgctc aagtgtatag ccaggcccaa atcgttctga aggttcgagg
      241 accaactgct gatgaattgc ctatgttgcg caaagattta gtgctaatcg ggttattgtc
      301 acctcatcac actgatggaa ttagcactct ggcaggacgg ggtgtaacag catttgcgat
      361 ggaaaagttg ccacgaattt cgcgagctca aagtatggat gtgctttcat cgcaagcaaa
      421 tattggtggt tataaggcag ttatcatcgc tgcggatacc tatcaaaagt tttttcccat
      481 gctcatgacc gcagcgggaa cagttaaggc ggcaagagtt ctggttctag gagccggtgt
      541 tgctgggttg caagcaattg cgacagcgaa gcgcttgggt gcagttattg aagcattcga
      601 tgtacgtcca gcggtaaaag agcaagttga aagtctaggt gcaaaattcg ttgaagtccc
      661 actgacagat gaagaaaaag caaaagcaaa agcaaaagca gaaacagctg gtggctatgc
      721 aaccgagatg tcggacgatt ataagcgtcg gcaaggggaa ttaattaatg aacgagcagc
      781 tgcggcagat atcatcatta ccacggcttt aattcctggg cggccagcgc ctgtattgat
      841 taaagaagaa actgtaaaag caatgaaacc gggctctgtg attgtcgata tggctgttga
      901 agcgggaggg aattgtccct tatctgagct gaataaaacg gttaccaagt atggtgtgca
      961 tttaataggg gttgctaatt taccaggttt agttgctgcg gatgcgagtg ctttatatgc
     1021 gcgtaactta atgaatttct taaatctgat gttggatgca aaaactggtg aactcaatat
     1081 taatcttgaa gatgaaatta ttgcaggaac tatggtttgc tccggcggcg acttagctaa
     1141 aaaagcgtga tacttgaaca agttatttta agtgataaat cttatgagga ataaacatga
     1201 ttggtgatat tgatccaacg ataattcatc ttactgtatt cgtgctggct gttttcgttg
     1261 gctaccatgt cgtatggaat gtgacccccg ctttgcatac ccctttaatg tcggtaacga
     1321 atgcaatttc gagcattatt ttagttggcg cgatgcttgc tgcaggccca ttagaggctg
     1381 attggggagt ctggttggga gcgattgctg tggtgcttgc ttcaattaat gtatttggcg
     1441 gtttcctcgt tacgcagcgt atgttggaaa tgtttagaaa aaaagataag aaaggaaacg
     1501 cataaatgtc agctaatttt attgctctag cttatctcgt tgcagcagta ttttttatat
     1561 tagccctaaa ggggctaagc tcgcctttat catcccgtcg aggtaacttg tttgggatgg
     1621 ttgggatggc gatcgcggtt gcaacgacct tgacgattac ccagaattgg atgctgatat
     1681 tgggttgcat tgcaattggc ggtattatcg gttcaatcgt tgcgaatcgt gttcagatga
     1741 ctgcaatgcc ggagttagtg gcgttcatgc attcattggt cggtctagcg gcggtgttta
     1801 ttgctattgc tgcggtcaat aacccggtat cctttggttt gcccgagaca ctgcctatgg
     1861 gtagtaaatt agagctattt ctcggtactt tcattggtgc aatgacttgg tcaggttctg
     1921 tcatcgcgtt tctgaaatta tctggaaaaa tgagcggggc tcctgtgatt ttttcaggac
     1981 agcatgcgat taatttgttg cttgctattg tgatgattgg tttcgggttg tggttctttt
     2041 ttagcgagac aaccaactgg acagcattta ttgcaatgac ggctatcgcc tttgtgcttg
     2101 gattcctgat cattattcca atcgggggtg ctgatatgcc ggtcgtaata tcaatgctga
     2161 attcctattc aggctgggcg gcagccggaa ttgggttctc attaggcaat ccgatgctga
     2221 taattgcagg ttcattagtg ggtagctcag gtgctattct ttcttacatc atgtgtaaag
     2281 cgatgaatcg ccccttcctt tccgtcatac tgggtggttt cggcggagac gctggtagtc
     2341 aggctgcaag tgatggcact caaaagacct atcgcagtgg tagcgctgaa gatgcggctt
     2401 ttctaatgag caatgctgat agcgtgatta taattccggg ctatggcttg gctgtggctc
     2461 gtgcacagca ttctgtcaag gaattggccg aggcgcttca taagaaaggg gttaatgcgc
     2521 gtttcgccat tcatcctgtg gcaggtagaa tgccgggcca catgaatgta ttgttggctg
     2581 aagctgaaat tccttatgag caagtccaag aaatggaaga aattaatagc gatttctcaa
     2641 ctacagatgt tgtattagtg ctaggagcaa atgatgtggt taatcctgcg gcaaatgttc
     2701 cagggagtcc aatctatggc atgcctattc tagaagcaca taaagcacgt actgttattg
     2761 tagtaaaacg cagcatggca gcagggtatg cgggcttgga taatgatttg ttctatatgg
     2821 acaaaacgat gatggtattt ggcgatgcga agaaagttgt tgaagatatg gttaaatcaa
     2881 tttagccttt gaggatgcga ccagagttgc ttcattgaaa tatcgattca ttttttgttg
     2941 attcttgtat tgagccattc tttagcagct ttctgtgctt gaacgagctg cgctggattc
     3001 aatgaacgag ctactttatc acgtgcgaga atagcttgtt cataccctcc agcggttgac
     3061 aaattgagcc acatatagct cttttcatta tcaatattga caccttgtcc aatatgatag
     3121 agcaagccta gttcatattg ggcgggtgca tggttctgtt ctgcagcgag actgaaccat
     3181 ttcaccgctt cttcaaaatc ttgcaatgtt ccatttcctg tcaagtatag ttgaccaagt
     3241 tgatgctgag cttccgcatt gccctcatta gctctattta gaaaaagatt aatcatgtga
     3301 tggtgagcat catcagaact atattgagtt tcggctggca ttgattgatt aactacacca
     3361 ttctgttgag tgtttgttgc ttcaattgct gctttaggat aggttgtggg aaaactatta
     3421 gatgactggt ttgcagtaga ttgatttgaa gcaagttgct tatcaaattt ttttccgtct
     3481 atcgatattt cgtcgagtga aataatgatc cagataaatg taattaagag caaactacag
     3541 ataaaaacaa cggatgcaac aatataagca tcacgatttt taacccagaa acgtgcttta
     3601 caatcacgac atctcatcgg cataagattc cagttagagg gtgaacgctc tccaggtctt
     3661 aatctggatc ggtgaatacg attactacca caatcaggac agtgattatt caagtagtat
     3721 ttattgcgag tcgctttttg tgaattggtc atttactatg agaaatcaat tatctaaatc
     3781 gcttttactt ggcgcggcta agcaaaaaga aggtgaataa taaaaaatta ctacttgcac
     3841 tgattattca taactgtttc taaaaaagaa atatcgatcg acgctgattt gggttcggga
     3901 aaaaaagaat ttgcaaggat ggtcgcaggc ttattaggtt ctcctaaatc cgttaaaact
     3961 acggccgcac ctttgaagtc atgtgaatgt gtatattcac tcgttgtaac aatagtagtg
     4021 atattcgctg taagcgaagc tctaagccca ttttctgaat cctctatagc gatgcactcg
     4081 gctggcgtta aatcgagcct atcaagaacc caatgataaa tatcaggagc gggtttctta
     4141 tgagggacaa tatctccagc gccaataaca tcaaaccaac ttggtgaatc ttcacctaag
     4201 gttgacttta aaagtgcata aacattttct ggggttgtag ttgtggcgat cgctatttta
     4261 atagcgttat tgcgcaattc ttgaattagc ctagcaacgc caggacgtag ggggatttta
     4321 ccttctgaca ataattgtaa gaaatatttt gttttggttt tgtgtaattc cgcaacccag
     4381 tcagttaagt tggtattttt cgatatttct ggcgaataat gatcaatgta atagcgtatc
     4441 ctttccttgc caccagtaat ttggagcaat ttaccataga gctcgatatc ccaattccaa
     4501 ttgagtccaa gttctttaaa agcggaattg aaagcaagac gatgtccatc tcgttctgtg
     4561 tcagcaaggg ttccatcaac atcaaataaa actgcagata atggcattaa ttattacctt
     4621 ttattatatt agcgtatcgc aaaatgatgg cttgcacgtt gtaatcgatc atgaacagca
     4681 agccaatcgg gaagattggg aggcatttcg actaataatc gatcatagcc ctcatcatct
     4741 aagtgatgaa gtttggcata taaatgatag ccataatcaa atggatcaga aggcattcga
     4801 tggtgtttaa gggacttaat tattttctca gtggtttgat gttgtgaata aaatgcttca
     4861 gaccaagaga gaatagcgac gtggtatttt tgattcaata gctcatcaac tcggtgttgg
     4921 agagtttctg ttggccaaac ttcgagggga gttctgggtg cgtaatggga cgcaagggtt
     4981 cccgaggctc gtaaattatt gctatgattg actgagacaa ttatattttg attaagtatt
     5041 tcttctattg cattcaaagg aatgcctccc ggtctgagga gaacaggttt tagatcgcaa
     5101 aaactgataa tcgtactctc taaccctatt ttacaaggcc ccccatccag tatcatactt
     5161 acttcagcac tgatttcttt actgatatga agcgctgctg ttggacttaa gcagccaaat
     5221 cgatttgctg aaggggctac gactgctttt tgagggccta atgcttttaa taaagcgata
     5281 gcaactggat gatcaggtat gcgcaaacct acggtatctt gaccacccgt tacacaataa
     5341 ggtacgtgcc gacttttggg aagaattatc gtaagggggc ctggccaaaa atattcgatt
     5401 agtttcttgg cttggctggg tatattttct gcccaatggc ttatttgccc caaatctgca
     5461 aaatgaacga tcagagggtg attagtggga cgttccttga tttgaaatat tcgattaact
     5521 gcgctgaggt gagtgatgtc agctcctaac ccgtatactg tttcagttgg gaaaacaatg
     5581 ttattgccag tttgtaacag tctagccgct ttgaaaattt gttctttaag caagtcagtt
     5641 ttagcaattg acgtcgtaat agccatttgt tgtcactaaa tgagaatgtt gtatttaatt
     5701 ccttgtgtca gccttatttt aatctaattc ctcggatgaa tggattatgg attaagaacg
     5761 ctaaaagtaa ttttaagaaa aatattcttc ttggtggttc attttctctt gaaagaaata
     5821 attacaaaga aaagttaaat ctttaaaaaa agaattttaa ccctttgttt tggaaaagct
     5881 ataaagccct attctagagg tgattatcct aaattcccgg aggggtgatg gaaaaactac
     5941 ttgactaaaa tggataaggt gactagcctg tagggtgt
//
LOCUS       DAOQLJ010000134         5973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_5760_length_5973_cov_5.635857, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000134 DAOQLJ010000000
VERSION     DAOQLJ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5760_length_5973_cov_5.635857"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PK522_09105"
     CDS             complement(<1..221)
                     /locus_tag="PK522_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41929.1"
                     /translation="MSQISFEDMACPLPITTHPKVLMAHGGGGRLMNQLIDTIFRAIF
                     TEVQPHDGAVMAFPQDKLVMTTDSFVVRPL"
     gene            complement(218..1312)
                     /gene="hypD"
                     /locus_tag="PK522_09110"
     CDS             complement(218..1312)
                     /gene="hypD"
                     /locus_tag="PK522_09110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNC41930.1"
                     /translation="MKFVDEFRDAHAAKKYTDAIHNLVTRPWTIMEICGGQTHSIVRF
                     GILDLLPAGINLVHGPGCPVCVTPLETIDKALAIAAKPNVIFCSFGDMLRVPGSHSDL
                     QSIRAAGGDVRIVYSPLDCLAIAQNNPEHAVVFFAIGFETTAPGNAMLVKRAEQLGLR
                     NLFVLVSHVLVPPAIEAILSSPQNNVQGFLAAGHVCTIMGMQDYEVLAQKYQVPIVVT
                     GFEPLDILQGLYMCIKQLEQGTVRAENQYSRSVLEQGNHPAQQIIAEVFQVLPRKWRG
                     MGEIPQSGWGLSEKYRAFDAEQHFGLGAMQVNEPPECISGQVLRGVKKPFECAAFGTR
                     CTPDHPLGATMVSGEGACAAYYKYRREEIA"
     gene            complement(1309..1545)
                     /locus_tag="PK522_09115"
     CDS             complement(1309..1545)
                     /locus_tag="PK522_09115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNC41931.1"
                     /translation="MCLAVPGEIVRIEGASPLEQMAQVRFGAVEKSVCLAFVPQAQVG
                     DYVLVHAGIAISIVQQDEVQRIFTYLDEIGELDP"
     gene            complement(1536..3782)
                     /gene="hypF"
                     /locus_tag="PK522_09120"
     CDS             complement(1536..3782)
                     /gene="hypF"
                     /locus_tag="PK522_09120"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNC41932.1"
                     /translation="MTRNEIIRRRYALKGAVQGVGFRPFVYHLANELGLKGWVRNDSA
                     GLIIEVEGDETKIADFEKQLQDHKPLNSIVSHIESFDLTPQLEVEFRILSSDSNPEIT
                     TLILPDLAICQDCIREMNDTADRRYRYPFINCTQCGPRFSITTALPYDRPNTSMAAFE
                     MCPECEREYQDPLSRRFHAQPIACAHCGPQLELWDQQGNSVAAGESALSQAIMQIEQG
                     NIIALKGLGGFHLVADATHDHAVKTLRARKHRKTKPFAVMYSSLEAIQADCVMAAEEK
                     VLLCSAAAPIVLVLRKPGAALSPWIAPDNPYLGVMLPYTPLHYLLLQALKRPIIATSG
                     NRASEPICIDNQEALAQLRGCADYFLVHNRPIINRVDDSIVRIMAGKTTLLRRSRGYA
                     PLPIELNEATQQPILAVGGHLKNTVAYMQGNRLMLSPHIGDLSTSKAMAAHEQAIVAF
                     TDLYRNSPEQVAHDAHPDYCSTRIAQAKQINCLSVQHHYAHALACMADNRIKAPCFAV
                     VWDGTGYGDDGTIWGGEFLQITAEGYQRLLHFLPFPLPGGDAAIREPRRSALGILYTL
                     QVDPDCLHFSAATRRFLWQALQQQINAPQTSSVGRLFDAVAALLGLCDQNQFEGEAAM
                     LVEFTAMQSDHKLVYPYHLEQGMIDWRPMLLAMLNEREKDKAWMARQFHNTLAAIIVA
                     AARLQDQRHVLLTGGCFQNKLLLETAQFELRQAGFEPYWHHRIPPNDGGLAAGQVMAA
                     LRVNQSCA"
     gene            complement(3769..4035)
                     /locus_tag="PK522_09125"
     CDS             complement(3769..4035)
                     /locus_tag="PK522_09125"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNC41933.1"
                     /translation="MHEFSLMADLFRKIREISNDNNNRKIVTVRVKLGALSHITPHHF
                     EEHFIDFSKGTPADGAKLEIVQLDDRNDPNAQSILLESVDLDAQ"
     gene            complement(4028..4507)
                     /locus_tag="PK522_09130"
     CDS             complement(4028..4507)
                     /locus_tag="PK522_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNC41934.1"
                     /translation="MTAKILIIGIGNRYRGDDGLGCFIVDELKKQVTHDVEVIEHNGD
                     PASLIDLWQGRSQVILIDAVSSGAEIGTIHYVDLRQQALPDTFQSYSTHGLGVAEAVE
                     LARVLGKLPPHIVFFGIEGENFETDAQQPVRLQQAITALQDRIMKAINTEIGESQRA"
     gene            complement(4504..5808)
                     /locus_tag="PK522_09135"
     CDS             complement(4504..5808)
                     /locus_tag="PK522_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HNC41935.1"
                     /translation="MDQENKTRTIKVDYLARVEGEGALYLKIQEGKLEEVQLKIFEPP
                     RFFEGFLRDRHFSEAPDITARICGICPIAYQMSAVHAIENAFNITIGGALRDLRRLVY
                     CGEWISSHALHIFMLHAPDFLGYESAIHMAKDFPEKVEQGLQLKKVGGMLMSLVGGRE
                     IHPINIRVGGFYRAPSKQELQTLVPALKQAREDALETVRWVSSFDFPDFEQHYTFISL
                     RHENEYPINEGRIVSNHGLTISASAFDDYFEEIHVAHTNALHCVLKADHHPYHVGPMA
                     RYALNFDRLPEPIQWKAKQAGLENVCRNPFKSIIVRAIEVLYACEEALRIIDTYTAPA
                     VSFVEIEPKPATGYAVTEAPRGILYHRYQIDGEGDIVDCDIIPPTSQNQKMIEEDLKQ
                     FVPRYLKLSDERLQWYAEQAIRNYDPCISCATHFLELNVDRS"
     gene            complement(5792..>5973)
                     /locus_tag="PK522_09140"
     CDS             complement(5792..>5973)
                     /locus_tag="PK522_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41936.1"
                     /translation="YQRGCYGCFGPMETPNMSALGDWLLQKGMSQKDWVRSLRAFNAN
                     SPAFKEESHKHGSRE"
BASE COUNT         1590 a         1540 c         1310 g         1533 t
ORIGIN      
        1 agcggtcgca ccacgaagga atcggttgtc atcactaatt tatcctgtgg aaatgccatg
       61 accgcaccat cgtgcggctg aacttcagta aatatcgccc gaaatatggt gtcgatgagc
      121 tgattcatga ggcgtccacc gccaccatgg gccatcaaca cttttggatg tgtggtaatc
      181 ggcaatgggc aagccatatc ctcaaacgat atttgactca cgcaatttcc tcccttcgat
      241 atttataata ggcagcgcag gcaccttcac cggataccat ggttgcaccg agcggatgat
      301 ccggcgtgca gcgcgtacca aacgctgcac attcaaaggg cttcttcact ccgcgtaaca
      361 cttgcccact gatacattca ggaggttcat taacttgcat cgcgccaagt ccaaaatgtt
      421 gttccgcatc gaaggctcga tatttttcgc tcaaccccca gccgctctgc ggaatctcac
      481 ccattccacg ccatttacgc ggtaatacct gaaaaacttc agcgatgatt tgctgtgcag
      541 gatggttacc ttgctcaagc acggaacggc tatattgatt ttcagcgcgc acggtgccct
      601 gctccagttg tttgatacac atataaagtc cttgcagaat atccaagggt tcaaagccag
      661 tgaccacgat cggtacttgg tatttctgcg cgagcacctc ataatcttgc attcccataa
      721 tggtacagac atgcccagca gctaagaaac cctgaacgtt attctgcggg gatgaaagaa
      781 tggcttcaat cgctggaggc actaacacat gcgacacgag cacgaataaa ttacgcaaac
      841 ccaattgttc agcgcgtttc actagcatcg cattaccggg cgccgtcgtt tcgaaaccaa
      901 tggcaaaaaa aacaaccgcg tgttctggat tgttttgtgc aatcgctaag caatcgagcg
      961 gtgaataaac aatgcgcaca tcgccacccg cggctcggat cgattgaaga tcgctgtgcg
     1021 atccaggaac tcgcagcata tcgccaaacg aacaaaagat aacattcggc tttgccgcaa
     1081 tcgcgagcgc tttatcaatc gtctcaagcg gcgtcacaca caccgggcaa ccggggccat
     1141 gcacgagatt gatccccgcg ggtaataaat ccagaatacc aaagcgcacg atggaatgcg
     1201 tttgaccccc gcaaatctcc atgatcgtcc aaggtcgcgt gactagattg tgaatggcat
     1261 ccgtgtattt tttggctgcg tgcgcgtctc gaaactcatc gacaaatttc atggatccaa
     1321 ctccccgatc tcatctaagt aggtaaaaat acgctgaact tcatcctgct gcacgatact
     1381 gatcgcgata cctgcatgga ccaacacata gtctccgact tgtgcttgcg gtacgaaagc
     1441 cagacaaacc gatttttcaa cggcgccaaa ccgcacttgc gccatttgct ccagaggcga
     1501 cgccccttcg atccgtacta tttcacctgg cacggctagg cacatgattg atttaccctc
     1561 aatgcagcca tgacctggcc tgctgcaagc ccaccatcat tgggtggaat acgatgatgc
     1621 cagtagggtt caaaaccagc ttggcgtaat tcgaattgtg ctgtttcaag caatagcttg
     1681 ttttgaaaac acccgccggt tagcaaaaca tggcgctgat cctgcaagcg tgcagccgct
     1741 acgatgatcg ctgcaagcgt attgtgaaac tgacgtgcca tccacgcctt atctttctct
     1801 cgttcattca acattgccag caacattggt ctccaatcaa tcatcccttg ttcaaggtga
     1861 taaggatata ctaatttatg atccgattgc atcgcggtaa attcaactaa catcgccgct
     1921 tccccttcaa attgattctg atcgcataac ccaagtaagg ctgcaacggc atcaaataat
     1981 cttccgacac tggaagtttg gggggcattg atctgctgtt gtaacgcttg ccataagaat
     2041 cgccgcgtgg ctgctgaaaa atgcaggcaa tcgggatcga cttgcaatgt gtagagaatt
     2101 cccaatgccg atctgcgtgg ttcacgaatc gccgcatccc cccctggcag aggaaacggt
     2161 aggaaatgta gtaaacgctg ataaccttca gcggttattt gcaaaaactc ccctccccag
     2221 atcgtaccat cgtcgccata accggtacca tcccaaacca cagcaaaaca cggcgcctta
     2281 atgcgattat ctgccataca ggctaatgca tgcgcgtagt ggtgttgcac cgataagcaa
     2341 ttgatctgct tggcttgcgc tatccgagtc gaacagtaat cggggtgggc atcatgggcg
     2401 acttgctcgg gtgaatttcg ataaaggtca gtaaaggcta caattgcctg ctcatgggcc
     2461 gccatcgctt ttgaggttga aagatcgcca atatgagggc tcagcattaa ccgattccct
     2521 tgcatataag caaccgtatt ttttagatgc ccacccacgg ctaggatcgg ctgctgcgtc
     2581 gcttcgttta gctcaatggg caaaggtgca tagcctcgac ttctgcgcaa taaggttgtt
     2641 ttccctgcca tgatgcgcac aatcgaatca tccacccggt ttataatcgg gcgattatgg
     2701 actagaaaat aatctgcaca acctcggagt tgcgcgagcg cttcctgatt atcgatacag
     2761 atgggctcac tcgcccgatt accactggtc gcgataattg ggcgctttaa tgcctgcaac
     2821 agcagataat gcaagggcgt ataaggcaac ataacgccta gataaggatt atctggagcg
     2881 atccacggcg aaagtgcagc gcctggtttg cgtaacacca agacaatcgg tgccgcagca
     2941 gaacataaca acactttttc ttcggcagcc ataacgcaat cggcttgtat tgcctccaaa
     3001 gaagaataca tcactgcaaa tggctttgtt ttgcgatgtt tacgcgcgcg aagtgttttc
     3061 acggcatgat catgcgttgc atcagcaacg agatggaatc ctcccagccc ttttaaggca
     3121 atgatgttac cttgttcgat ctgcatgatt gcctgactca acgcactttc accagctgct
     3181 acgctattac cctgctgatc ccaaagctcc agctgtggac cacaatgggc acaagcaatc
     3241 ggttgcgcat ggaagcgacg gctcagcgga tcctgatatt cacgctcaca ttccgggcac
     3301 atctcaaacg ctgccatgct ggtattggga cgatcatagg gcagtgcagt cgtgatcgag
     3361 aagcgtggac cacactgggt acaattgata aaaggatatc gatagcgacg atctgcagta
     3421 tcgttcattt cccgaataca atcttggcaa attgccagat ccggaagaat cagtgtcgtg
     3481 atttcaggat tactatcact tgaaagaatc ctgaattcga cttcaagctg gggcgttaga
     3541 tcgaatgatt cgatatggct gactatcgaa ttcagcggtt tatggtcttg gagttgtttt
     3601 tcaaagtcag cgattttagt ttcatcacct tccacttcga taataagacc tgcgctatca
     3661 tttctaaccc atcccttcaa acccaattca ttcgccaggt gatagacaaa gggtcgaaat
     3721 ccaacgccct gaactgcgcc ttttaaggca tagcggcggc gaatgatttc attgcgcgtc
     3781 aagatcgaca ctttctagca aaatagactg tgcattcgga tcattacgat cgtcgagttg
     3841 aacgatttct aatttggcgc catcagcagg ggtgcctttt gaaaaatcaa tgaaatgttc
     3901 ctcaaaatga tgcggggtaa tatgtgataa cgcgcccagc ttcacacgta ccgtaacgat
     3961 cttgcgatta ttattatcat tagaaatctc acgaattttt cgaaacaaat ctgccatcag
     4021 cgaaaattca tgcacgctga ctctctccta tttcagtgtt gattgctttc attatacgat
     4081 cttgcagcgc tgttattgct tgctgcaatc ggacaggttg ctgggcatca gtctcaaaat
     4141 tttcaccttc aataccaaaa aaaacgatgt gaggtggtaa tttaccgagg actcgcgcta
     4201 actcaaccgc ttcagcaacg cccaatccat gggtcgaata actctggaac gtatcaggca
     4261 acgcttgctg acgcaaatca acataatgga tggttccgat ttcagcgccc gaagaaactg
     4321 catcgatcaa aatgacctgc gatcgtcctt gccataaatc aatcagcgat gcaggatcgc
     4381 cgttatgttc gatgacctcc acatcgtggg taacctgttt tttaagctca tcgacgataa
     4441 aacagcctaa cccatcatca ccccggtaac gattgccgat cccaatgatg agtatttttg
     4501 cagtcatgat cggtcgacgt tcagttctaa aaaatgcgtt gcacacgata tgcacggatc
     4561 gtaattacgg atcgcttgtt cagcatacca ttgtaagcgt tcatcggata acttcagata
     4621 gcgcggcacg aactgcttta aatcctcctc gatcattttc tgattctgag acgtaggcgg
     4681 aataatgtca caatctacga tatccccttc accatcgatt tgatagcgat gataaagaat
     4741 gcctctcggt gcttctgtta ccgcataacc ggttgctggt ttgggttcaa tttcaacaaa
     4801 tgacacagcc ggcgctgtat aggtatcaat gatccgcaag gcttcttcac aggcatagag
     4861 aacttcaatt gcacgtacga taatactttt gaaaggattg cgacaaacat tctccaatcc
     4921 tgcttgtttc gctttccatt gaataggctc tggcaaacga tcaaaattca gtgcataacg
     4981 cgccatgggt cccacatgat agggatgatg atccgctttt aacacacagt gcaacgcatt
     5041 ggtatgcgcg acatgaattt cctcaaaata atcatcaaac gctgatgctg aaatagttag
     5101 gccatgattg gagacaattc tcccttcgtt tattgggtac tcgttttcat ggcgtaatga
     5161 tataaacgta taatgctgct cgaaatcggg aaaatcaaac gaggagaccc atcgcacggt
     5221 ttcaagtgca tcctcacgcg cctgctttaa tgctggcacg agcgtttgta attcttgttt
     5281 actgggagca cggtaaaaac cacccacccg aatattgatg ggatgaattt ccctcccccc
     5341 cacaagactc atcagcattc cacccacttt ttttaactga agtccttgct caactttttc
     5401 tggaaaatcc tttgccatat gaatggcact ctcatatcct aaaaaatcag gggcatgaag
     5461 cataaaaata tgcagtgcat gactggaaat ccattccccg caataaacca gtcggcgcag
     5521 atcacgcaaa gcgcccccaa ttgtaatatt gaaggcattc tcgatagcat gcactgcact
     5581 catctgatag gcaatcgggc aaataccgca gattcttgct gtaatgtcgg gtgcttcact
     5641 aaaatgacgg tctcgcaaga atccttcaaa aaaccgaggg ggctcaaaaa ttttgagttg
     5701 aacttcttcc agctttcctt cttgtatctt cagatacagc gcaccttccc cttccacccg
     5761 cgccagataa tcgactttaa tggttctggt tttattctct tgatccatgt ttatggcttt
     5821 cttctttgaa agcaggtgaa tttgcgttaa aagcacgaag tgagcgcacc caatccttct
     5881 gggacatccc tttttgtaat aaccagtcgc ccagcgcgct catattcggt gtttccattg
     5941 gcccaaagca accatagcaa ccacgctgat aag
//
LOCUS       DAOQLJ010000135         5870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_5935_length_5870_cov_6.536887, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000135 DAOQLJ010000000
VERSION     DAOQLJ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5935_length_5870_cov_6.536887"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1952)
                     /locus_tag="PK522_09145"
     CDS             complement(<1..1952)
                     /locus_tag="PK522_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNC41937.1"
                     /translation="MSTPIQTNTLNSTQAKIISLIATELTAKTPQVSAAIKLLDEGAT
                     VPFIARYRKEATGNLDDTQLRTLQERLLYLRELEERRQVILSSIEQQGKLTEQLRNTI
                     EQATTKQLLEDIYLPYKPKRRTRAQIARLAGLEPLAEALLTNPLLNPELEAEKYIRIE
                     PATDQSEAINVPTAKAALEGARDILVEKFSETAELLATMRAKLWNEGILSTTVIPGKE
                     QAEEEKFRDYYAYSEPIRTIPSHRALALFRGQALDVLRIDLKLADTQEELDFHPCAAL
                     IAKQFHIDNKNRPADKWLLEVCQWTWRVKLHTQVCVELFMQLREAAETEAIRIFSKNL
                     QELLLAAPAGAKVVLGIDPGFRTGCKIAVVDKTGKLVETTTIYPHQPQNCWKESLLTI
                     TQLVTQHKVELIAIGNGTASRETDKLTDEVIKFIEASSSHLRLAKIVISEAGASIYSA
                     SAFAAAEFPDLDVSLRGAVSIARRLQDPLAELVKIEPKSIGVGQYQHDINQRALTRAL
                     DATVEDCVNAVGVDINTASAPLLARVSGLNRTLAQNIVQYRDTTGRFINRQAILNVPR
                     LGNKTFEQAAGFLRITDGNNPLDRSAVHPEAYPVVERILNKLKKGISEVMKQPEALKG
                     LSPEEFTDETFGLLTIRDILSELEKPG"
     gene            complement(2006..3157)
                     /locus_tag="PK522_09150"
     CDS             complement(2006..3157)
                     /locus_tag="PK522_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNC41938.1"
                     /translation="MDFDEENLFPHQDALAQLHENRPLSEKLNFLHQLIREDFPFIDR
                     LAIALLDEKTRTLKTYTHSTDDGNSPITAYEASLDSAPSLLEIIKRRRPRLVQDLAIF
                     NKGAHQHTKLIAAKGYKASYTMPLYQSGVFVGFLFFNSYESHPFLPPILRQIDIYGHL
                     IALMVISELTAIRTLLSAVKAARSMTHYRDVETGSHVDRTAHYARLIAKELAQHYQLR
                     DEQIEHIFLFSPLHDIGKIGIPDSILRKPGKLDDQEFAVMKTHPGKGREIIDSILKDF
                     SLGTFGHVEILRNIAEYHHEAIDGSGYPNQLQGNEIPIEARISAVADVFDALTSSRTY
                     KAAWSNEEAFAMLQRLSENKLDKDCVEALIKNKDKILEIQQLFRDSEDK"
     gene            3366..3551
                     /locus_tag="PK522_09155"
     CDS             3366..3551
                     /locus_tag="PK522_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41939.1"
                     /translation="MTLKHNQHGVSSHSSKVTIGTSEFGENKPQTKSIKQNTTTHLSQ
                     MEDASWHRSKSARFQQN"
     gene            3593..4465
                     /locus_tag="PK522_09160"
     CDS             3593..4465
                     /locus_tag="PK522_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HNC41940.1"
                     /translation="MKTPKQIESLIEEGLVDSVICQLMSGKEALVYVVQCGSELRCAK
                     VYKETHRRNFRHSANYTEGRYLKNFRRTREIQKRSCFGKVAREEAWQNTEINALCRLS
                     AAGVKVPRYINFFEGVLLMELITDSQGNVAPRLNDIRLTTEQAKSYHTLLINQVVRML
                     CRGMIHGDLSEYNVLLGSQGPVIIDFPQAIYAVENNNARWLLKRDIDNITKYFSHFSP
                     ELAKTDFGSEIWSLYERGKLDWDTPLTGVLVETHKPVNIDGLLKTIDSVMKKELAWQR
                     YKQERWTSQRSLAF"
     gene            4856..5446
                     /locus_tag="PK522_09165"
     CDS             4856..5446
                     /locus_tag="PK522_09165"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC41941.1"
                     /translation="MKKVLNVGGNNKSIPLPPQYVEFEHLLLDIDPRGEPDFVCDARN
                     LATLDADQFDAIYCSHNLEHYYRHDVKKVLNGFLHVLNENGFVHIRVPDIQEVMRITM
                     ERNLDLDDFLYQSSAGPIMVSDVLYGYSVEIERSGQDFFSHKTGFTQKSLEKALQNAG
                     FYKIYTTTRNLEIVALAFKGVPDSYVSSLFDLPLLS"
     gene            5494..5870
                     /gene="tnpA"
                     /locus_tag="PK522_09170"
                     /pseudo
     CDS             5494..5870
                     /gene="tnpA"
                     /locus_tag="PK522_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044282811.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         1637 a         1146 c         1300 g         1787 t
ORIGIN      
        1 cctggctttt cgagttcaga taaaatatcg cggatagtta gcagaccaaa agtttcatcc
       61 gtaaattctt caggggaaag tccttttagc gcctcgggtt gtttcatgac ctcactaatc
      121 ccttttttga gcttatttaa aattcgctca acgacaggat acgcttctgg atgaacagca
      181 gagcggtcaa gcggattgtt accatcggta atgcgtagga aaccagccgc ttgctcgaat
      241 gttttatttc caagtcttgg aacattcaat atcgcttgcc gattgataaa acgacctgtc
      301 gtatcacgat attgcacgat attctgtgct aacgtgcgat tcagtcctga tacacgcgcc
      361 aataacggtg cagaagccgt gttaatatca acacccactg cattaacgca atcctcgact
      421 gttgcatcaa gtgcacgtgt tagtgcgcgt tgattgatat catgttgata ttgcccaaca
      481 ccaatggatt taggttcgat tttaacgagt tcagcaagag ggtcttgtag gcgacgtgca
      541 atcgacacgg ctccccgcag actaacatca agatcaggaa attctgcggc agcaaaggct
      601 gaagctgagt aaatagaagc tcctgcttcg ctaatcacaa ttttagcgag ccgaagatga
      661 gaagaacttg cttcgataaa tttaataact tcatccgtca acttatctgt ttctctgctc
      721 gcagtaccat tgccaatggc aatcaattca accttatgtt gggtaactaa ctgtgtaatc
      781 gttaatagcg attctttcca gcaattttgt ggttggtggg gataaatcgt agtcgtttca
      841 actaattttc ctgttttatc aacaactgca attttacaac cggttctgaa tcctgggtca
      901 atgcctagta cgactttagc gccagcaggt gctgctaata gtagttcctg taagtttttg
      961 ctaaaaatcc tgattgcttc agtctcagca gcttccctta attgcatgaa cagctcaaca
     1021 cagacttgag tatgtaattt aactcgccag gtccactgac atacttctag cagccatttg
     1081 tctgcaggcc gatttttatt atcaatatga aattgtttag cgatgagcgc tgcgcaggga
     1141 tgaaaatcaa gctcttcctg ggtatctgct aattttaaat cgattcgaag cacatcgagt
     1201 gcttgccctc gaaagagtgc taatgcccga tgagagggga tcgttcgaat cggctcggaa
     1261 taagcgtaat aatcacgaaa tttttcttct tcagcttgct ccttaccggg aattacagtt
     1321 gtcgagagta tgccttcatt ccatagcttc gcgcgcattg tggcaagtaa ttcggccgtc
     1381 tctgagaatt tttccactaa gatatcacgc gcaccttcta gtgcagcctt ggcagttgga
     1441 acattgatcg cttcactctg atcggttgct ggctcgattc gaatatattt ctcagcttct
     1501 aattcaggat tgagtaaagg attcgttaat agcgcttcag ctaacggctc taatccagct
     1561 aagcgcgcaa tctgtgcgcg agtacggcgt ttgggtttat aaggtagata gatatcttcg
     1621 agtaattgct tggtagtggc ttgctcaatc gtgttgcgca gctgttccgt tagttttccc
     1681 tgttgttcaa ttgaggatag aatgacttgc cttcgttctt cgagctctcg taaataaagc
     1741 aaacgttctt gcaacgtgcg cagttgtgta tcatcgagat tacctgtggc ttctttgcga
     1801 tagcgagcaa taaagggaac cgtggctccc tcatcgagta gtttgatggc tgcggagact
     1861 tggggtgttt ttgcagttaa ttcagttgca atcaaactaa taatttttgc ttgcgtagaa
     1921 tttaacgtat tcgtttggat gggtgtgctc atttttgaag ggtatcaagg acaaattgtg
     1981 ggatcgtgat tttgattgtg acaagttatt tatcttctga atcacgaaag agttgttgta
     2041 tctctaatat cttatcttta tttttgataa gcgcttccac acaatcttta tcaagtttgt
     2101 tttcggacaa gcgctgcaac atggcaaatg cttcttcgtt cgaccaagcg gctttatacg
     2161 ttcgactgga agtcaaggca tcaaatacat cggcaacggc actgattctg gcttcgatag
     2221 gaatctcatt accttgaagt tggttaggat aaccgctgcc gtcaatcgct tcatgatggt
     2281 attctgcgat attgcgcaaa atttcaacat gtccaaatgt acctaaacta aaatctttca
     2341 agatactatc aataatttct cgtcctttgc cagggtgggt tttcataacg gcaaattctt
     2401 gatcatctaa tttacccggt ttacgcaaga tgctatctgg aataccaatt ttcccgatat
     2461 cgtgtaacgg agagaacaaa aaaatatgtt cgatctgctc atcacgcaac tgatagtgtt
     2521 gtgccagttc ttttgcaatc aaacgcgcat aatgcgctgt acgatcaacg tgggagccgg
     2581 tttccacatc gcgatagtgc gtcatactac gcgctgcttt tactgcggag agtaatgtgc
     2641 ggatggccgt taattcactg ataaccatta gcgcaatcaa atgaccataa atgtcgattt
     2701 gacgcagaat cggtggtaag aatggatgag attcataaga gttaaaaaat aagaatccta
     2761 cgaatacacc actttgatac agtggcatgg tatagctcgc cttatagcct ttggctgcga
     2821 tcaattttgt gtgctggtgg gcacctttat taaaaattgc taaatcctga acgagtcgag
     2881 gacgacggcg cttaatgatt tccagcaggg atggggcgga atcgagagaa gcttcatagg
     2941 cagtaatcgg gctattgccg tcgtcggtac tgtgggtata ggttttgagt gttctcgttt
     3001 tctcgtcaag cagcgcaatc gccaaacgat caataaacgg aaagtcttca cggattagct
     3061 gatgtaaaaa attgagcttc tcggatagag gacggttttc atgtagctgc gctaatgcgt
     3121 cttgatgtgg aaataagttc tcttcgtcaa aatccatatt cgatgaatga ctctattcta
     3181 ttgtttatga aagttgtaag gcgctatttt aatcgatttg cgactaatgg tctgcttcaa
     3241 tcatgcgctg cgacaaattg tcgcgcggca atatgccaca ttccaaagta atttttttaa
     3301 ctttacaatg cagccatgag taaatcattg cagtaatgaa tgaccttact aactaggaga
     3361 tccgtatgac gctcaaacat aatcagcatg gtgtttcgtc tcactcaagc aaagttacaa
     3421 ttgggaccag tgagttcggt gaaaacaagc cacaaacgaa atcgatcaag cagaatacaa
     3481 cgacacattt aagtcagatg gaagatgcat cctggcatcg cagtaaatca gcacgttttc
     3541 aacaaaattg agcaattaat caattgtttt ctaatataga gatgagacaa aaatgaaaac
     3601 ccctaaacaa atcgaatcat tgatagaaga aggtttagtt gattcagtga tttgccaact
     3661 catgagtggt aaagaagcgt tggtttatgt tgtgcaatgt ggaagtgagc tgcgatgtgc
     3721 caaagtatac aaagaaacac atcgacgcaa ttttcggcat agcgcaaatt atactgaagg
     3781 ccgttacttg aaaaattttc gtcgtacacg tgagattcag aaacgaagct gctttggaaa
     3841 agtagctcga gaagaggcct ggcagaatac tgaaattaat gcattatgcc gactgagtgc
     3901 agcaggtgta aaagttccaa ggtacattaa tttcttcgag ggtgtgcttt taatggaact
     3961 tatcacggat tcccaaggta atgtggcacc tcgtttgaac gatatcaggt taacgacgga
     4021 acaagcgaaa tcctatcaca cattactgat taatcaggtg gttcgtatgc tatgtcgtgg
     4081 catgattcat ggtgatttat ccgaatacaa tgtattgctt ggttcccaag gacctgtaat
     4141 catcgatttt ccgcaagcca tttatgcagt agagaataat aatgcgcgtt ggcttttgaa
     4201 gcgagatatt gacaatatca caaaatattt tagtcatttt tcccctgaac tcgctaagac
     4261 agattttgga tcggaaattt ggtcactata cgaacgcggg aaactagatt gggatactcc
     4321 attgacaggc gtattggtag aaacacataa gccagtcaat attgatgggc tactaaaaac
     4381 gatcgattcg gttatgaaga aagaactggc gtggcagcgc tataaacaag agcgatggac
     4441 aagccagcga agcttagctt tctaaaagga atctcttttt tattactcgg ttaatagaaa
     4501 ttattaattt ttacgcaaca agttagattt ttgtgtctcc ctcctagcgg cgcgtttaag
     4561 tgattcttcc ccccaccttg atcatttaaa atcacgcgcc gcttctttat gatgtttgcc
     4621 atcaaattgt tattttgacc tacgttttcg cttgcaaata taaattcagc tttttgcaaa
     4681 gtgcctgaac gcagtacact gcatttttta ttgtctgtgt actgtcagtt tatggtgtta
     4741 gatagcgaga gaagctagaa ataaccggtt tttctcgcat agttccaaaa gaaaatatta
     4801 tctgtattag ttaaattaac agctctttga cgttatatat acaaggaagc tgaaaatgaa
     4861 aaaagtcctg aacgtagggg gaaacaataa atcgatccca ctgccgcccc agtatgtaga
     4921 gtttgaacat ctcctgctcg atattgaccc gcgaggcgag cctgattttg tgtgcgatgc
     4981 acggaatttg gcaactctgg atgctgacca gtttgatgca atctattgtt cacataacct
     5041 tgagcactat tatcggcatg acgtcaagaa ggttctgaac ggtttcttgc atgttctgaa
     5101 cgagaatgga tttgtccata tccgggtgcc agatattcaa gaagttatgc gcattacgat
     5161 ggagcggaat ctagatcttg atgatttttt gtatcaatct tccgctgggc cgattatggt
     5221 gtcagatgtt ttatatggct atagtgtcga aatcgagcgt agtgggcaag atttcttcag
     5281 tcacaaaaca ggttttactc agaaatcact tgagaaagcg ctacaaaatg caggtttcta
     5341 taaaatatat actacaacaa gaaatcttga aattgttgcg ttagcgttta agggcgtacc
     5401 tgacagctat gtaagctctc tttttgatct accgttgctc agttgaattt ctttggatga
     5461 tatttcctgc cgcttgcagc gtagaatttg tggatgagcg aatatataca taagagtcat
     5521 aatgtcaagt tttactgtac tacttagtat ttctggcaag atacagaggt gtagtttttg
     5581 atgagcaagt taatgttgtt ttcaaaggta tttgccttga attggcagag cgctatcaag
     5641 tgaagttttt agagattggt actgataagg atcatcttga tttcttggta tagtcagtgc
     5701 cgacataagt gctactagct tattaagagc ataactgcac gagaaatttt caagcgttac
     5761 cctcgttaag aagaaactat ggggcgaaga attttgggct gatggttatt ttgcgagtac
     5821 ggttagtaat tttgagacca aataccccgc tacttgtggc gggggtcttt
//
LOCUS       DAOQLJ010000136         5796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_6050_length_5796_cov_7.155374, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000136 DAOQLJ010000000
VERSION     DAOQLJ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6050_length_5796_cov_7.155374"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="PK522_09175"
     CDS             <1..1264
                     /locus_tag="PK522_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNC41942.1"
                     /translation="ANNQLFNASESINQEQFAVRYRKNVAPGQLITITTHALHRDFQN
                     LLPFTNGGQVEFNRWVGGAMLQYSSDHTLWQRPNRLLAGIDYGIQNDYRQRYNNHFGE
                     RGLLNLNQIERVQNIGPFLRNEWRIFDRLDFVVGGRWDWLHYQVKDTFLADDNQSGSR
                     TLSQGSGTAGLVYHLGDAHQLYTHVATIFEAPTTTELINNPTGKGGFNPNLNAQTSLS
                     KEMGIRGQPSGFEYEATLFHVGTRNEITPFELPDSPGRAFFRNAGQSERFGLETRLSS
                     PSWNGFRADMTYTYSDFEFKRYRVGNDDLSGNEFPGLPRHRWEGRVRYTHPVGFFTQF
                     HVHRIGEFFVNDINTVTNSAYYLGQFLLGWEKKYHWIEGSIFLGINNLFDERYNANTR
                     INAALGRYFEPGPPLNVFGGARIRLWVF"
     gene            complement(1373..2653)
                     /locus_tag="PK522_09180"
     CDS             complement(1373..2653)
                     /locus_tag="PK522_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNC41943.1"
                     /translation="MNKNIKMMLFGFVSLLLLCCGSLVIAAEPASSVNEARLVAQYNY
                     TIHILAMLLVGFGFLMVFVRRYGFGATTGTYLVVATGLPLYMLLRANGIFGHELNPHS
                     VDAILYSEFAVATALIAMGAVLGRLRVFQYALLALLIVPAYLLNEWLVLDNASGLTEG
                     FQDSAGSIVIHAFGAYFGLAMSLVLTTAQQREEPIVSDATSDRFAMLGSMVLWLFWPS
                     FATAIVPFEDMPQTVVNTLLALSGATLATYFLSTHFHKGKTSMVDMANAALAGGVAIG
                     STCNVVGPTGAFMIGLLAGALSVIGYVFIQPWLESKLKLIDTCGVHNLHGMPGLLGGL
                     SAIVVVPGIAVVQLTGIGITLAIAITGGLIAGAIIKATGTTEQAYEDSHEFTHLEGPE
                     SETRVEQLALDAKKGVDDLKREVSSRATASQPRF"
     gene            complement(2766..4721)
                     /locus_tag="PK522_09185"
     CDS             complement(2766..4721)
                     /locus_tag="PK522_09185"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNC41944.1"
                     /translation="MSDLAAIFSAQGVLAHTIPNYRARIEQLEMAQAIAKTIQHQQIL
                     VAEAGTGTGKTFAYLVPALLFGGKVIISTGTKTLQDQLYDRDIPTIRAALKVPVTVAL
                     LKGRANYVCHYRLEQTLESDQYKLTTREDIIHLGLIERFAQNHQTGDKSSLSKVPENA
                     SIWQHVTSTRDNCLGSDCPHFKQCFVMEARKKALLSEVIVVNHHLFFADLMLRDEGLS
                     ELLPTCNTVIFDEAHQLPEIASLFFGESVSTSQLLELARDSEMETRLAAPDYKPLLDC
                     IEAFEKTTRDLRLTIEQDNARLSLNHALRSNHFSAALALLQQRLTELVNLLEHQAVRS
                     PGLEQCWKRTTIIHSSLNRWQDQLEQEGYVRWVETFNQALHLNATPLSIAETFSKQLS
                     VPPRAWIFTSATLAVKDDFSHYQQAMGLAQAQSIRWESPYNFAHQALLYVPTGLPSPN
                     HQHFTAALIEAVLPVLKASQGRAFILCTSLRAMHEIHALLQTQFEQHQLTYPILLQGQ
                     GSRSTMLERFRELDNAILIGSQSFWEGIDVKGEALSVVIIDKLPFASPDDPVLSARID
                     QLTKEGHNAFMEYQLPRAIINLKQGAGRLIRDEHDRGVLMICDPRLITHAYGKRIWQS
                     LPPMKRTRNFLDVQKFFSPQPIQPINS"
     gene            4850..>5796
                     /locus_tag="PK522_09190"
     CDS             4850..>5796
                     /locus_tag="PK522_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNC41945.1"
                     /translation="MNIPLFQFIVFILLLSLSVFSVDHAVAQQINLKKEMKELPIHVE
                     ADRIEGFAERHMEAIGKAQILQGDQFIAADRIKYYRGTQEVEVKGKVRFERDRDILEG
                     SNLKLFLDTKTGELSLPQYILKDGGGRGAGQLFLFEGDDQYRLKKGNYTTCPIGNDDW
                     YIRAEDLEIDNKKQVGTARDVTVRFKDVPILYLPWMDFSYSGQRKTGLLAPIFGNTIR
                     SGVEVSIPFYWNIAPNIDATFTSRNMSRRGAMLNTEFRYMGQSHGGQLNFDILPNDLE
                     TGKTRYGLAFEHMQHLGGGWTGNINYNQISDREYFRDLAT"
BASE COUNT         1621 a         1179 c         1387 g         1609 t
ORIGIN      
        1 ggctaataat caattattca atgccagtga gtcgattaat caagagcaat ttgccgttcg
       61 ttatcgcaaa aatgttgcac caggccagtt aattacgatt actacccatg cacttcatcg
      121 cgattttcaa aacttacttc cctttactaa tggtggacag gtcgagttta atcgctgggt
      181 aggcggtgcc atgctgcaat attcaagcga tcatacttta tggcagcggc ctaatcgctt
      241 attggccgga attgactatg gtattcagaa tgactatcga caacgctata acaatcattt
      301 tggtgaacgg gggttattaa atctcaatca aatcgagcgc gtgcaaaaca tcggcccctt
      361 tctgagaaat gaatggcgaa tattcgatcg cctagatttt gtcgtagggg ggcgttggga
      421 ttggttgcac tatcaagtca aagacacgtt tctcgctgac gataatcaat caggctcaag
      481 aaccttatcg caaggaagtg gcacagccgg tttggtatat cacttagggg atgcacatca
      541 gctttatacc catgttgcca cgatatttga agcgccaacg actactgagc tcatcaataa
      601 tccgacaggt aaggggggct tcaatcctaa tctaaatgcc caaacttcgt tgagtaaaga
      661 aatgggtata cgcggccagc cttcaggatt tgaatatgaa gcaacgttat ttcatgttgg
      721 aactcgcaac gaaattactc cttttgaact acccgattca ccaggacgtg ctttttttcg
      781 taacgcgggc cagtccgagc gctttggctt ggagacccgt ttatcgagtc cctcatggaa
      841 tggttttagg gccgatatga cctacactta ctccgatttc gaatttaaac gttatcgagt
      901 tggcaacgat gatttaagcg gcaatgagtt tcctggttta cccagacatc gttgggaagg
      961 cagggtacgt tatacccatc cagttggatt cttcacacaa tttcacgtgc accgcattgg
     1021 ggaatttttt gtgaatgata tcaatacggt tactaatagt gcttattatc ttgggcaatt
     1081 cttattaggc tgggaaaaga aatatcactg gattgaaggc tcgatctttt tgggaatcaa
     1141 taatttattt gatgaacgtt ataatgcaaa tactcggatc aatgcagctt taggtcggta
     1201 ttttgagcct ggccctccgc tcaatgtttt tggaggagcc aggattcgat tatgggtatt
     1261 ttgatatctc gggttaattg tcaacacgct cggccaagct aaccgtgtgc gcacaatcta
     1321 gcttggcgac gacagtatca attgatcacg agagtgttat agtgattttc tattagaatc
     1381 gcggttgaga agcggttgct cgactactaa cctcgcgttt aagatcgtct acccctttct
     1441 tagcatcgag tgccagttgt tcgacacgcg tttcgctttc cggtccttct agatgagtaa
     1501 attcatggct atcttcatag gcttgttcag tcgtgcccgt tgctttaatg attgctccag
     1561 caataagacc gccggtaata gcaatggcga gtgtgatgcc gatgcctgtc aattgcacaa
     1621 ccgcaatacc aggaacaacg acaattgcac ttagaccacc gagtaaacca ggcatcccat
     1681 gtaaattatg gacaccgcaa gtatcaatta atttaagttt ggattcgagc cacggttgaa
     1741 tgaaaacata gccaatgacc gatagtgcac cagcgagcaa accgatcatg aatgccccgg
     1801 ttggccccac aacattacac gttgagccga tcgcaacccc accggctaat gctgcgttag
     1861 ccatatcgac catagaagtt ttacctttat ggaaatgagt actcaagaaa taagtcgcaa
     1921 gggttgcacc actgagcgcg agcaaagtgt tgactactgt ttgaggcata tcttcaaatg
     1981 gtacgatcgc tgttgcaaaa ctaggccaga acaaccataa caccatggaa cctaacatag
     2041 caaaccgatc agaggtagca tcagaaacga tgggttcttc gcgttgctgc gcagtggtta
     2101 gcacgagaga catggcaagt ccgaaatatg cgccaaacgc atggattacg atggatccag
     2161 cagaatcctg aaatccttca gtcaaaccag atgcattgtc caataccaac cattcgttga
     2221 ggaggtaagc aggtacgatc aataatgcta acagtgcata ttgaaatacc cgcaagcgac
     2281 ctagcacagc gcccatggcg atgagtgcgg ttgctaccgc aaattcagaa tataagatag
     2341 catcgactga atgcggattt aattcatggc caaagatacc attggcacgg agtagcatat
     2401 aaagcggtag tccggtagca actactaaat aggtaccggt tgtggcacca aacccatagc
     2461 ggcgcacgaa taccatcaaa aaaccaaacc cgaccaataa catggcaaga atgtgaatcg
     2521 tataattata ttgcgcgaca agccgcgctt cattgacgct cgaagccggc tcagcagcaa
     2581 taacgagcga tccacaacac aggagaagca aactgacgaa gccaaataac atcattttaa
     2641 tatttttgtt cattataatt tcccttatga attgaggagt tggtttgggt tgccccgcta
     2701 cgcagtgtga accttaatgg ccctaaattc aataggggaa taaaagagaa tttttgataa
     2761 ttgaattaag aattgatggg ttgaattggc tgcggactaa agaatttctg gacatcgaga
     2821 aaattccgtg tacgtttcat cggaggtagg ctttgccaga tgcgtttacc gtaagcgtgg
     2881 gtgatcagac gaggatcgca aatcattagt acaccacgat cgtgttcatc acgaatcagt
     2941 cgcccagcac cttgtttaag attgatgata gcacggggta attgatactc catgaaggcg
     3001 ttgtgtcctt ctttggtaag ttgatcgatg cgtgccgata ataccggatc gtcaggggag
     3061 gcaaatggta atttatcgat gattacgact gatagtgcct cacctttcac atcaatgcct
     3121 tcccaaaaag actgactgcc gatgagtatt gcattgtcta attcacggaa gcgctctagc
     3181 atggttgatc gcgatccttg tccctgaagc agaattggat aagttaactg gtgttgttcg
     3241 aattgcgttt gtaataaggc atggatctca tgcatggcac gtaagctagt acataatatg
     3301 aaagcacgac cttggcttgc ttttagcacg ggcaggaccg cttcgataag tgcagccgtg
     3361 aagtgttgat ggttagggct gggcaagccg gttggcacgt agagtaatgc ttgatgcgca
     3421 aaattgtaag gactttccca acgaatcgat tgcgcttgtg ctaaccccat cgcttgttgg
     3481 taatgagaaa aatcatcctt gacggcaagc gtggccgaag tgaatatcca agcgcgtggt
     3541 ggaacactga gctgcttgct aaatgtttct gcgatagaaa gcggcgtggc attgagatgg
     3601 agtgcttggt tgaaggtttc aacccagcgt acatagcctt cttgttcgag ctgatcttgc
     3661 caacgattga ggctactgtg aatgattgta gttcgtttcc agcattgctc taaccctgga
     3721 gaccgaacgg cttgatgttc cagtagattg actaattcag tcagtcgttg ttgaagtagc
     3781 gcaagtgcag cgctgaaatg attggagcgt agcgcgtgat tgagggataa acgggcatta
     3841 tcttgctcga tggttaagcg taaatcgcgc gttgtttttt cgaatgcttc gatacaatcg
     3901 agtaacggtt tatagtctgg agcagcgagt ctggtttcca tttcactgtc acgtgccaac
     3961 tctaataact gactcgtgct cactgattct ccaaaaaaga gactagcaat ttccggtaat
     4021 tgatgcgctt catcgaaaat aacggtattg caggttggaa gcaattcaga gagtccttcg
     4081 tcacgcaaca tcaagtctgc aaagaataaa tgatgattga cgacaatgac ttcagacaat
     4141 aatgcttttt tgcgtgcttc cattacgaag cattgtttga aatgaggaca atcagaacct
     4201 aaacaattat cacgggtcga tgtgacatgt tgccaaatgc tcgcattttc aggaacttta
     4261 cttaagctgc ttttatcgcc ggtttggtga ttctgggcga aacgttcgat gaggccaagg
     4321 tggatgatat cttcgcgtgt ggttagctta tattgatcgg attcgagggt ctgttcaagg
     4381 cgataatgac agacatagtt ggcgcggcct ttgagtaaag caacggtaac cggaacttta
     4441 agtgctgcgc gtatcgtcgg gatatcgcgg tcatagagtt gatcttgtaa cgtcttggtt
     4501 cctgtcgaga taataacttt tccaccgaat aacaaagcag gcacaagata agcgaaggtt
     4561 tttccagtac cagtgcctgc ttcagcgacc aagatttgtt ggtgctgaat cgttttggct
     4621 atggcttgcg ccatttctaa ttgttcaatt ctggctcgat aattaggaat cgtgtgggca
     4681 agaacacctt gtgcagaaaa aatagctgca agatcagaca tgaaaatgat tgtcagttaa
     4741 aatagcggtt ttgagtaatc gcgaataaag ttgtccaata atgagtgtta tctaccttaa
     4801 gcttgctgcc attgcaaatt tgctttgaaa tggcttcggt atcgcattaa tgaatattcc
     4861 tttgtttcaa ttcatcgttt ttatattgct gctaagtcta tcagtgttta gcgtagatca
     4921 tgcggttgcg cagcaaatca atttaaaaaa ggagatgaaa gaattaccca tccatgttga
     4981 agcggatcgt atcgagggtt ttgctgaacg gcatatggaa gccattggca aggcgcagat
     5041 tttacaaggc gatcaattca ttgctgcaga tcggataaag tattatcggg gaactcagga
     5101 agtcgaagtt aaaggtaaag tccgttttga acgggatcgg gatattctcg aaggaagtaa
     5161 ccttaagtta tttctggata ctaaaaccgg tgaattaagt cttcctcaat atattcttaa
     5221 agatggcggt ggacgcggtg ccgggcaatt atttttattt gaaggggacg atcaataccg
     5281 actgaaaaaa ggtaattaca caacctgtcc gattggtaac gatgattggt atattcgggc
     5341 tgaagattta gaaattgata ataaaaagca agttggaacc gctcgggatg tgacagttcg
     5401 gtttaaagat gttcccattc tttatttgcc ctggatggat ttttcctata gtgggcagcg
     5461 taaaacagga ctactggcgc caatttttgg taatacgatt cgcagtggtg tcgaagtatc
     5521 gattccgttt tactggaaca ttgcaccaaa catagacgca acgtttacat cgcgtaatat
     5581 gagtcgacgg ggcgcaatgt taaatactga atttcgctat atgggccaga gtcatggcgg
     5641 tcaactcaat tttgatattt taccgaatga tttggagact ggaaaaaccc gttatggcct
     5701 tgcttttgag catatgcaac atttaggagg cggttggact ggcaatatta actataatca
     5761 aatttcggat cgtgagtatt ttcgagattt agcaac
//
LOCUS       DAOQLJ010000137         5700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_6222_length_5700_cov_5.981754, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000137 DAOQLJ010000000
VERSION     DAOQLJ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6222_length_5700_cov_5.981754"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PK522_09195"
     CDS             complement(<1..1166)
                     /locus_tag="PK522_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41946.1"
                     /translation="MRNVVTAPYKRLVINFHWLICLICLVTITGCLSPIALNRAVIAY
                     DDAVTDVVSQQLLINIVRAHHRQPIHFTGVSNIAATFTFQASAGAAPAAGGLAGSSIL
                     PIFGGSMAESPTISIVPIEGEDFTRRLLTPFPQSGLILLLRQHFDVDLLLRMMAQEVR
                     LQHKPYHSDTFRNKSTDKAGYEMFRRIVLHLSAIQDQNQLYVEPLPLTHTWKIPAATI
                     APDSFQSLQKEFVVHYDSADDTFTLHKQILGPILITNYDPDTLSESERELLNERAREW
                     DVSDVAFDIRAGYYGGEWPMVGIFRLRSFHSILSFLSKALGNETGYHVEKDPRTPPIL
                     NNENPDLTMELIQSNIAPSGVDFSIRWNNQFYAVNTTGPHARWNRDAFQLLFLL"
     gene            complement(1218..3296)
                     /locus_tag="PK522_09200"
     CDS             complement(1218..3296)
                     /locus_tag="PK522_09200"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNC41947.1"
                     /translation="MDHSFFNLYHHGFVRVAVGVPDVRVADPVFNVNETIQLMKQAAE
                     QHAALILFPELGLTAYSCEDLFHQQALINSSVEALQSLLDQSKHINIITVVGLPLQIN
                     QLLFNCAVVIYGGRILGVVPKTYLPNYREFYELRQFSPASAASSDVISLCQQTDIPFG
                     ERLLFSANNQPSFKFFIEICEDLWVPIPPSSQAALAGASILLNLSASNITMGKHEYRQ
                     NLVANQSARCLAAYLYATAGVGESTTDLAWDGHALVYENGTCLAESERFRQSSQLILA
                     DLDLDRLKQERMRQTSFSQSVQYYKESIRQFREIKFDIYFPEVERLLCQRHYSRFPFV
                     PSDAHQRDKHCLEAFNIQVQGLIKRLKHTGIQKIVVGISGGLDSTHALIVACRVMDEL
                     HYPRHHVLAYTLPGYATSGQTKANALALMNSLGCTSHEIDIRPSCEQMLKDIDHPFSR
                     GESVYDTTFENVQAGERTNHLFRLANYHHALVLGTGDLSELALGWCTYGVGDHMSHYA
                     INASMPKTLIQFMIRWVADAQLFNAETSELLNAILATEFSPELIPGNTTDNQPYQKTE
                     SIIGPYELQDFHLYYTIRYGYLPTKIAFLAYCSWRDRSVGNWPGIPQDRQNQYTIGEI
                     KHWLKVFLYRFFQTSQFKRSCIPNSPKVGSGGSLSPRGDYRAPSDSEATIWLSNAELI
                     PDIAPPDILK"
     gene            complement(3737..3949)
                     /locus_tag="PK522_09205"
     CDS             complement(3737..3949)
                     /locus_tag="PK522_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41948.1"
                     /translation="MDRELSFDADNFDLDLSELKELEDIVADGMIPDSRIVKIIDEIE
                     FAKEDNFDVFEDLEDTEISLSRFNLD"
     gene            4244..5518
                     /gene="lplT"
                     /locus_tag="PK522_09210"
     CDS             4244..5518
                     /gene="lplT"
                     /locus_tag="PK522_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HNC41949.1"
                     /translation="MERGFYTIMAAQFFSSLADNALLVVAIALLIDLNAPAYLTPMLK
                     FVFVLFYVVLAPFVGAFADSMAKGRVMFVSNTIKIAGCSLLFFAMHQYFALAAYAIVG
                     LGAAAYSPAKYGILTELLPPEQLVTANGWIEGLTVASIVLGTVLGGVLITPSVATLIL
                     SFDFPLIDTGIDSAVEASIIIIGVFYAIAALFNLFIPDTGVDHRILKFNPLFLVHEFS
                     HCVKLLWADKLGQISLAVTTLFWGAGATLQFIVLKWAEAALNFPLNQAAQLQGVVALG
                     IAIGAVLAAKIVSLRQSVKVIPLGILMGIVVMVMTLVNNLWIAIGLLIIIGGLAGFFV
                     VPMNALLQHRGHILMGAGHSIAVQNFNENLSILVMLLLYALLIWLDVHIYVVIILFGA
                     FVSISMTIIRKWHLTNQSKEDSLHLIGMRKIH"
     gene            complement(5515..>5700)
                     /locus_tag="PK522_09215"
     CDS             complement(5515..>5700)
                     /locus_tag="PK522_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNC41950.1"
                     /translation="DSISNLRGRLHSYLGTPLIVTYHPAYLLRSLMDKSKAWEDLCFA
                     KATMESLAITTNPSLKI"
BASE COUNT         1654 a         1226 c         1162 g         1658 t
ORIGIN      
        1 aatagtaaaa ataacaattg aaaagcatct cgattccagc gagcatgggg gcctgttgta
       61 ttaaccgcat aaaattgatt attccagcga attgaaaaat caacacctga cggcgctata
      121 tttgactgaa tcaattccat cgtaagatct gggttttcat tattcaagat gggtggcgtt
      181 ctcggatctt tttcaacatg atagcccgtt tcattaccta gcgctttact gagaaaactc
      241 agaatagaat gaaaactcct cagtctaaaa attccgacca tgggccattc cccgccatag
      301 taaccagccc gaatatcaaa agcgacatcg ctcacatccc attcacgagc acgttcgttc
      361 agtaactctc gttcactctc agataacgta tctggatcat agttggtgat caaaatagga
      421 cccagaattt gcttgtgaag cgtaaacgta tcgtcagcac tgtcatagtg gacaacgaat
      481 tctttttgca acgattggaa actgtctggc gcaatggtag ctgcgggaat tttccaagta
      541 tgcgtcagag gcagtggttc aacgtagagc tgattctgat cttgaatggc tgataaatgc
      601 agaacgatgc gacgaaacat ttcatacccg gctttatcag tggatttatt gcgaaacgtg
      661 tcactatggt aaggtttatg ctgcaaacgg acttcctgcg ccatcatgcg gagtaacaga
      721 tcaacatcga aatgctgacg cagcaataat atcaacccac tttgcggaaa cggtgtaagc
      781 aatcgccgcg taaaatcttc tccttcgatg ggaacaatgc taatcgtcgg actttctgcc
      841 atactcccac caaaaattgg cagtatcgat gagccggcca acccacctgc ggccggagca
      901 gcaccggcac ttgcttgaaa cgtaaatgtt gcagcaatat tagaaacacc tgtgaaatga
      961 atgggttgcc gatgatgcgc gcgaacgata ttaatcaata actgctgtga taccacatcc
     1021 gtcaccgcat catcataagc gatcacagca cgattcaacg caatcggcga taaacaaccc
     1081 gtaatcgtaa ccaagcagat caagcaaatt aaccagtgaa agttgatgac taaacgcttg
     1141 tatggggcag ttactacgtt tctcatccca gtcgaggaat catacttgcg aataaacata
     1201 tgagcgttgg taccttatca tttcagaata tcagggggtg ctatatctgg aatcaattca
     1261 gcattactta accaaatcgt tgcttcacta tcgctgggtg cccgataatc tccccggggt
     1321 gagagtgagc ctcctgagcc aaccttgggt gaattcggta tacaactgcg cttgaattga
     1381 ctcgtttgaa aaaatcgata tagaaaaact ttcaaccaat gtttaatttc tccaatcgta
     1441 tattggttct gcctatcttg cggaattcca ggccagtttc ctacagaacg atctctccaa
     1501 ctgcaatagg ctaaaaaagc gatttttgta gggagataac catatcgaat agtgtaataa
     1561 aggtggaaat cctgtaattc atagggacca atgatggact ctgttttctg gtaaggctga
     1621 ttatccgtgg tattaccagg aatcaattca gggctaaatt ctgttgctaa gattgcattg
     1681 agtagttcac tagtttcagc attaaataat tgcgcatctg cgacccaacg aatcataaac
     1741 tgaatcaaag tcttaggcat actcgcattg attgcgtagt gagacatgtg atcacccact
     1801 ccataagtac accaccccaa agctaattca ctgaggtcgc ctgttccgag taccaatgca
     1861 tgatgatagt tggccaagcg aaaaaggtga ttggttcgct cccccgcttg tacattttca
     1921 aaagtcgtat catagacaga ttccccgcga ctaaatgggt gatcgatatc tttcagcatt
     1981 tgctcgcagc taggtcgaat atcgatctca tgcgacgtac aacccaaact attcatcaat
     2041 gcaagggcat tggcttttgt ttggccactc gtcgcataac cgggtaacgt ataagctagc
     2101 acgtgatggc gcgggtaatg taactcgtcc atgactcggc aagcaacaat gagcgcatgc
     2161 gtcgaatcaa gcccacccga tatccccacg acaatttttt gaatgccggt atgctttaat
     2221 cgtttaatta aaccttgcac ctggatatta aatgcttcta ggcaatgttt atcgcgttga
     2281 tgcgcatcgg atggtacgaa tgggaagcga ctataatgcc gttgacaaag caatcgctcg
     2341 acttccggaa agtagatatc aaacttaatc tcgcggaatt gtcgtatcga ttccttataa
     2401 tattgaacac tctggctaaa actcgtctgc cgcatacgct cttgcttcaa gcgatccaga
     2461 tctagatcag ccaggatcaa ttgtgaactt tgtcggaagc gttctgactc ggccaagcaa
     2521 gttccatttt cataaacgag tgcatgccca tcccaagcaa gatcagtcgt tgattcacca
     2581 acccctgcag tggcatagag gtatgctgcc aggcaacgcg ctgattgatt agccactaaa
     2641 ttttggcgat attcgtgttt acccattgta atgttcgaag cagacagatt gagtaagatg
     2701 gaagcgccag ccaatgcagc ttggctcgaa gggggtatcg gcacccagag atcttcacaa
     2761 atttcgataa aaaatttaaa agaaggctga ttatttgcac taaataggag ccgttctcca
     2821 aagggaatat cggtttgctg gcataatgag attacatcgg atgaagcagc gcttgcaggt
     2881 gaaaattgcc ggagttcata aaattcacgg taattcggaa ggtaagtttt aggtacaacc
     2941 cccaagatac gaccgccata gattacaacc gcacaattga ataaaagctg attgatttgg
     3001 agcggtaacc caacaactgt aatgatattg atatgcttcg actgatcgag taatgattga
     3061 agcgcttcaa cactactatt gattagtgct tgttgatgaa ataaatcttc acaggaataa
     3121 gctgttaacc ctaattcggg aaaaagtatc aatgcagcat gctgttcagc agcttgcttc
     3181 atcaattgaa tcgtttcatt gacattgaat accggatcag caactcgaac atccggaact
     3241 cccactgcga cacggacaaa accatggtga tacaagttga agaaagagtg atccatcggt
     3301 atcaaatccg tgatgagtag gccttaatta gcaagcgtaa gcttttacac tttcatttat
     3361 tagcaagcaa gaaatggtag gccaatatga ctatttaccc tattgtttag ctaatttgaa
     3421 ttaatttcaa caaattatcg gggtgaattt tcctgcccta cccctatgat tgactctacc
     3481 gaaagtcaaa attagcgtca actgatgttt tacaagcttt atcgtttctc agtcagtaaa
     3541 atgactggaa aacaatcgat ggattacaag atgggagtaa caacacgcga ttaatacgac
     3601 tagctgcgcc acttcatcgc cgtatcattt taaatttaca aagcaaaatc gctactaacg
     3661 attagtatcg tagactagcg attcaggcca gttgtagcat aagaaacgtt aaactagcct
     3721 tacctattta agtcatttag tctaaattga atcgacttaa cgaaatttct gtatcttcaa
     3781 gatcttcaaa aacatcaaaa ttatcttctt tagcaaattc gatttcatca ataattttaa
     3841 cgattcgact atctggaatc atgccatctg ctacgatatc ctctagctct ttaagctcac
     3901 ttaaatctag atcgaaatta tcagcatcaa aagaaagttc tctgtccatt ttgcaaacct
     3961 ccttaacact ttatttgaag acaatatatt aatatcacgt tcaattacaa aacaaagcag
     4021 aaacaattag tgttttacag tacttattag acttttgaat gacttcgatc gttctaacaa
     4081 ttaacaaatt tactgatgat gtatataaat ttccaaaaga aaaataaccc aggaagatgc
     4141 ttaatcctga tcagcttgtt ttcacatagg aatattaata cacattttgt tcatatcaat
     4201 ttcagatact atttttaaaa tattaacttt aaggagcatt agcttggaac gcggatttta
     4261 taccatcatg gcggcacaat tcttttcgtc actggctgac aatgcgctgt tagtggttgc
     4321 cattgcatta ttaatcgatc tcaatgcacc cgcatacctt actcccatgc tcaaatttgt
     4381 atttgtttta ttctatgtag ttcttgcacc cttcgtcggg gcatttgccg attccatggc
     4441 aaaaggacgc gtgatgttcg tcagcaacac aattaaaatt gctggttgtt cactactttt
     4501 tttcgcgatg catcaatatt ttgcattggc agcttatgcg atcgttggac tcggagctgc
     4561 cgcatattca ccggcaaaat atggcatatt gaccgagtta ttgccgccag aacaactcgt
     4621 tactgccaat ggttggattg aagggctcac cgtcgcatcg attgtgcttg gaacagtgct
     4681 gggcggtgtt ttaattacgc catccgttgc tacgctgata ctttcttttg attttcccct
     4741 tatcgatact gggattgatt ctgccgtaga agcaagcatt atcatcattg gggtttttta
     4801 tgcgattgca gccctattta atttattcat ccccgatact ggggttgatc atcgcatcct
     4861 taaatttaat ccccttttcc tagttcatga attctcacac tgtgtcaaat tgctctgggc
     4921 tgataaattg ggtcaaattt ctctcgcagt tactacccta ttctggggtg caggtgcaac
     4981 gttacaattt attgtattga aatgggctga agcagcactg aattttccac tgaatcaagc
     5041 agcacaatta cagggggttg ttgcactcgg tattgcgata ggtgctgttt tagctgccaa
     5101 aatcgtttcc ctgcgtcaat cggtaaaagt cattccattg gggatactga tgggcatcgt
     5161 cgtgatggtc atgacactcg tcaataacct ctggatcgca atcggattac tcattatcat
     5221 cggtggtcta gcgggttttt ttgtggtgcc gatgaacgcg ctactgcagc atcggggtca
     5281 cattctcatg ggtgccgggc attccatcgc cgttcaaaat ttcaatgaaa atttgagcat
     5341 tctcgtcatg ttgcttttat atgcattgtt aatttggcta gatgtgcata tctatgttgt
     5401 gatcatctta tttggtgcgt ttgtctcaat ttcgatgaca attattcgca aatggcacct
     5461 aaccaaccaa agtaaagaag actcgctaca tctcattggt atgcgtaaaa ttcattaaat
     5521 tttgagcgat gggtttgtag ttattgcgag tgattccatc gtggctttgg caaagcataa
     5581 atcttcccaa gctttagatt tatccattaa ggatcttagg agatacgcag ggtggtatgt
     5641 cacgattaag ggtgtgccca aataagagtg caatcgcccc cgtaaatttg aaatactatc
//
LOCUS       DAOQLJ010000138         5685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_6251_length_5685_cov_5.702131, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000138 DAOQLJ010000000
VERSION     DAOQLJ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6251_length_5685_cov_5.702131"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PK522_09220"
     CDS             <1..941
                     /locus_tag="PK522_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41951.1"
                     /translation="DPDDDDNSGSGGGGSDPDDDDNSGSGGGGSDPDDDDNSGSGGGG
                     SDPDDDNSGSGGGGSDPDDDNSGSGGGGSGPDDNSGSGGGGSGSDDNSGSGGGGSDPD
                     DDNSGSGGGGSGSDDSSGSSSGSSEADESPFVESALNQVGIGFPSNAPRSNHSGLGDV
                     VTALTYNVFNHEPTGIIFDITGRIKAPTASAANNLGTGQVDYAIQGDLIKSMSRFMLS
                     ASFGYRILGNPSGVNFHNVFYGAAGIAFRYSPDTTIGTSFNIGQSPVRLQDSRDFTLY
                     VSHRLNDNFRLNIYGLRGASERSPDWGGGINLRYVF"
     gene            complement(1032..1484)
                     /locus_tag="PK522_09225"
     CDS             complement(1032..1484)
                     /locus_tag="PK522_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC41952.1"
                     /translation="MQQMQKGFTLIELMIVVAIIGILAAVAVPAYQNYTIKSRFTEVI
                     NATAPFKTAVELCVQSGNCLNGANIVVAATGARNQIEVPDDQTAAVGKIGAITVSNVG
                     VILATPVASQGVTAADTYQLTPARQANGTILWTIGGGCLNSTNAGKIC"
     gene            complement(1688..4480)
                     /gene="glnE"
                     /locus_tag="PK522_09230"
     CDS             complement(1688..4480)
                     /gene="glnE"
                     /locus_tag="PK522_09230"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HNC41953.1"
                     /translation="MILHGSHTTTESLLEKILPFSRYVQRLLASEPDLQGYLMDKGAS
                     QFLREEMLDFLNSACVAPSLNEAGLNAVLRKLRKRVMLRLAVRDLAGLAGLPEVMMTM
                     TQLAEVTIQFALKHHQIWMSHPEHYGEPIGEKSASPRQLLVIAMGKLGGGELNVSSDV
                     DLIFLYPEEGETNGKKSISNHEFFTRLGRKLIASLNDYTVDGYVFRVDMRLRPHGENS
                     PLTISFAMLEEYLTTQGREWERHAWLKSRVIVGSLAEEAILMEQIVRPFVFRKYLDFE
                     AYEAMRRLHAQLRKEVDRREMHDNIKLGPGGIREIEFITQVFQLIRGGRDADLCIRPT
                     LDTLGRLLEKQPLPSQTIQELIDAYQFLRKLEHRLQYLDDQQTQSIPENADDQALIAT
                     AMGFASPPELFAKLDIYRSQVTRHFELIFAVPRKSPTHDMLTALWQEQPSDLTQVKAA
                     THQLTTLGFSDPEKISARIQQFYVSQFFQKMPLASQQKITAVMPTLVEAVAQFPPIEL
                     TLERMLQLLEHISQHPAYLALLLEHPQTLPRVAKLVNSSQWASNYLGRHPILLDELLA
                     PQGLYQLPDWKALKVELMRQLNHINSPKKNITEWQMDVLRHFQHAQVFQLLAVDLEGV
                     LLLETLSDHLTELADLILGNVLNLAWQGLKKKHQEQPVFAIIGYGKLGGKELGYSSDL
                     DIVFLYDDNHPDALEIYTKLSQSINTWLTSHTSAGGLYETDLRLRPEGTSGLLACSLD
                     AFKHYQQSQAWVWEHQALTRARFVVGNEQMGAAFEAIRKEILCKPRDLTKLKQDILMM
                     REKMLDAHPNPTSLFDIKHDRGGIIDVEFMVQYLVLGYSNQFPQLTGNIGNIALLKLA
                     GNLGLIPSEMAQHVLSTYREYRRIHHRLRLSGDPELTGAASTKEASISYARIEATQLN
                     DARRVVLQLWEEVFAK"
     gene            4661..>5685
                     /locus_tag="PK522_09235"
     CDS             4661..>5685
                     /locus_tag="PK522_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HNC41954.1"
                     /translation="MNQQTPRHQPYSHSIARLSIVCLFALAALFSVTILALRYWILPN
                     IDNYRLEIAQIISKRAEQLVSIDRINAHWNGVHPYLQLHGVKVYDQAGTPALILTEVE
                     GTLSWHSILHQELRFREIKIDRPTLIMRRDAHNVIHIAGTNISDEKTDAENSFIDWLL
                     RQEKLVINQTDIYWLDEYRNAPALYLKVVNLLLHSHNLTNRHQLGLRVAALDSTAMSM
                     DIRGDFTGKSIESFLHWQGRLYFELKQIDLAVWRQWIPFPDEIDIRQGKGALRGWLTL
                     AEGTITDWLIDTALHQISANLGTNLPKLELDFLQGRIDVKQSNQSGSLVQEWTAHQLN
                     FKLAHQQ"
BASE COUNT         1608 a         1325 c         1190 g         1562 t
ORIGIN      
        1 ccgatcccga tgatgacgat aattccggtt ccggtggtgg tggttccgat cccgatgacg
       61 acgataattc cggttccggt ggcggtggtt ccgatcccga tgatgacgac aattccggtt
      121 ctggcggcgg tggttccgat cccgatgatg ataattccgg ctctggtggc ggtggttccg
      181 atcccgatga tgacaattcc ggctctggtg gcggcggatc aggcccagat gataattccg
      241 gttctggtgg cggtggatca ggttcagatg acaattccgg ttccggtggt ggtggttccg
      301 atcccgatga cgataattcc ggttccggtg gcggtggatc aggttcagat gatagctcag
      361 gctctagtag tggcagctca gaagcagatg agtctccatt tgttgaaagc gcactcaatc
      421 aagttggaat cggttttcca tcgaatgcac ctcgttcaaa tcattcaggg ttaggtgatg
      481 ttgtcactgc actcacttac aatgtcttca atcatgaacc gacaggaatc atttttgata
      541 tcacaggccg cataaaagct ccaactgcca gcgcagccaa taatttaggg actggacagg
      601 tcgattatgc gatacaaggt gatttaatca aatcgatgtc acgatttatg ctcagcgcga
      661 gctttggcta tcgaattctt ggaaacccgt caggggtaaa tttccataat gtgttctatg
      721 gcgctgcggg tattgcattc cggtattcac ccgataccac catcggcact agtttcaata
      781 ttgggcaatc gcccgttcga ctgcaagatt caagagactt cacgctttac gtgtctcatc
      841 gccttaatga taactttaga ctcaacattt acggactcag aggcgcgtca gagcgcagcc
      901 ctgactgggg cggtggtatt aacttacgct acgtgtttta aatgattgcc aactaaaaat
      961 ttgcctaaaa agtaaaagac aaaaaaatac cccattgctg gggtattttg taaaaagccg
     1021 ttatgaagtt cttaacaaat cttccctgca tttgttgaat tcagacaccc gccaccaatc
     1081 gtccataata tagtcccatt agcttgacga gctggggtta attgatatgt atcagctgca
     1141 gtaacaccct ggcttgcaac gggtgtagct agaataacgc caacatttga aacagtaata
     1201 gccccaattt taccaacagc tgcagtttga tcatctggta cttcaatttg attacgtgcg
     1261 cctgtagcag caacaacaat gtttgcaccg tttaaacaat tacctgattg cacacatagc
     1321 tctacagccg tcttaaatgg tgctgttgcg ttaatcactt ctgtaaatct agatttgatt
     1381 gtatagtttt gatacgcagg tacagcaacc gctgccaaaa taccaataat ggcaacgaca
     1441 atcattaact cgatcaatgt aaaacctttt tgcatttgtt gcatgtatca tctcctttgg
     1501 gttgaaaaaa aacacactcg cgtgtggtat aagccgtgta gtgaaggcaa acccctacac
     1561 tatttgttgt taatagaaca ctgctgtcac tttaacgact gacgattaaa atagtttaat
     1621 gagaataacg tgaagttatg cgcttatatc gaacgaatag ctgagaatta atcgtgataa
     1681 tgctttttca tttagcaaat acttcttccc agagctgaag cacaaccctg cgtgcatcgt
     1741 tcagttgagt ggcttcaatc cgtgcatatg aaattgaagc ttcttttgta gacgcagcac
     1801 ctgtcaactc gggatcccca cttaatcgca agcgatgatg tatacggcga tattcccgat
     1861 aagttgatag aacatgctgt gccatttcag atggaatcaa gccgagattg cctgcgagtt
     1921 tcaataacgc gatattcccg atattaccgg tcagttgagg aaactggttt gaataaccta
     1981 gcacgagata ttgcaccata aactcgacat cgatgatccc accacgatca tgtttgatat
     2041 cgaataatga cgtaggattg ggatgcgcat cgagcatttt ctctcgcatc atcaaaatat
     2101 cttgcttaag cttggttaag tcacgtggct tacaaagtat ttccttacgg atggcttcaa
     2161 atgcagcccc catttgttca ttccctacta caaagcgagc acgagtcaat gcttgatgct
     2221 cccataccca ggcttgactc tgttgataat gcttaaaagc atccaaagaa caagccaata
     2281 gaccactcgt accttcaggt cgcagccgta agtcggtttc atacaaacca cccgcagagg
     2341 tatgactcgt caaccaggta ttaatacttt gactcagctt ggtataaatt tccaatgcat
     2401 caggatgatt atcatcatag agaaaaacga tgtccaaatc tgaggaataa cctaattcct
     2461 taccgcctaa ctttccatag ccgatgatcg caaaaaccgg ctgctcttga tgtttcttct
     2521 taagcccttg ccaagccaaa ttcaatacat tccccaaaat aagatcagcg agctcagtta
     2581 agtgatcact cagggtttcc agcaacaaaa caccttccag atcaactgca agcaactgaa
     2641 aaacttgggc atgttgaaag tgcctcaata catccatttg ccactcagta atatttttct
     2701 taggactatt gatgtgattg agttgccgca tcaattcaac ttttagcgct ttccaatccg
     2761 gtagttgata aagtccctga ggcgctaaca gttcatcaag caaaatagga tgtcttccca
     2821 aataattact cgcccactga ctggaattca ctaatttcgc gacccgcggt aatgtttgag
     2881 gatgttctaa tagcaaggcc aaataagccg gatgctgact gatatgttcc agcaattgca
     2941 gcattcgctc tagcgttaat tcaataggtg gaaactgcgc cacagcttca actaaagttg
     3001 gcatgaccgc cgtaattttt tgttggctcg ctaaaggcat tttctgaaaa aattgactca
     3061 cataaaattg ttgaatccga gcggaaattt tctcaggatc actaaaccct aacgttgtta
     3121 attggtgtgt tgctgctttg acttgcgtca gatcactcgg ctgctcctgc cagagcgcag
     3181 taagcatatc gtgagtcggt gatttcctcg gtactgcaaa aatcaactca aaatgccgtg
     3241 tcacctggct gcgatagatg tccagcttag caaataattc aggtggactt gcaaaaccca
     3301 tagcggtagc gatcaatgct tgatcatcgg cattttcagg aatcgattgg gtttgctgat
     3361 cgtctaaata ttgcaatcga tgctcgagct tacgcaaaaa ttgataagca tcgattaatt
     3421 cttgaatagt ctgagatggc aaaggttgtt tctcgagtaa acgacctaac gtgtccagtg
     3481 tagggcgaat acacaaatct gcatcacgcc cgcctcgaat cagctgaaat acttgcgtga
     3541 taaattcaat ctcccgaatt ccaccggggc ccagtttaat gttgtcatgc atttcgcgcc
     3601 gatcaacttc cttgcgcaat tgtgcatgaa gtcgccgcat ggcttcatac gcttcaaaat
     3661 cgagatattt ccgaaaaacg aatgggcgaa cgatctgctc catcaaaatt gcttcttcag
     3721 ccaatgaacc aacaatcacg cgacttttga gccaggcatg tcgttcccac tcacgcccct
     3781 gagtcgttaa atactcctcc aacatcgcga aactaatggt aagtggacta ttttcaccat
     3841 gcggtcgtaa ccgcatatca acccggaaaa cgtaaccatc taccgtgtag tcattcaagc
     3901 tggcaatgag cttacgtcca agtcgggtaa agaactcatg attcgaaatt gatttttttc
     3961 cattggtttc accttcttca ggataaagaa aaatgagatc gacgtcggaa gagacattta
     4021 actcccctcc tcccaatttg cccatcgcaa taactagtag ttgtcgaggg gaagcacttt
     4081 tttcgccaat cggctctcca taatgttcgg ggtgagacat ccaaatctgg tgatgtttta
     4141 gcgcaaactg aatcgtgact tcagcaagtt gagtcatggt catcatgact tctggcagac
     4201 ctgccaaacc ggccaaatca cggactgcta gccgcaacat cactcgttta cgaagcttcc
     4261 gtaatactgc attcagcccc gcttcattca atgaaggagc aacgcaagct gaattcaaga
     4321 aatccagcat ttcttctcgc agaaattggg atgcgccctt atccataagg tagccttgca
     4381 aatctggctc actcgctaac aatcgctgaa catagcgact aaaaggaaga atcttttcca
     4441 gtagggactc agttgtcgtg tgggatccgt gtaaaatcat ggcaaaatag gcagcaaaat
     4501 tcgctaacta agtatataag aattattgtc aaaccagtca cgatgtatca ctcatcatga
     4561 agtgtacttt gtatgattac gactgcaaca cagctcattt tgctgtttct acgttaaccc
     4621 agttaagcta actgtaattt ataagaaact gaatactcca ttgaatcaac aaaccccacg
     4681 ccaccaaccg tatagccact cgattgcacg actttctatc gtgtgtctat ttgctttagc
     4741 tgcccttttt tctgtcacca tcctggcgct acgttattgg attttgccaa atattgataa
     4801 ttaccgattg gaaattgcgc agattatctc aaaaagggcg gaacaactcg tgagcatcga
     4861 tcgaattaat gcccattgga atggcgtaca cccttacctt cagcttcatg gggtaaaagt
     4921 ctacgaccaa gcgggtaccc ctgctttgat cttaactgaa gtagaaggaa cgctttcatg
     4981 gcattctatc cttcatcaag agctgcgttt tcgcgaaata aaaattgatc gcccaacgct
     5041 aattatgcgg cgtgacgcac ataatgtgat tcatatcgcc ggcactaaca tttccgacga
     5101 aaaaaccgac gcagaaaata gttttatcga ttggctgttg cgccaggaaa aacttgtaat
     5161 caatcaaacc gatatctatt ggcttgatga ataccgcaat gcaccggcgc tgtacctcaa
     5221 agttgtcaat ctactactgc atagccataa cttaaccaat cggcatcaat taggcttaag
     5281 agttgctgcc ttggatagta cagcgatgtc gatggatatt cggggagact ttacaggtaa
     5341 atcgattgag tcctttttgc attggcaagg gcgcctctac tttgaattaa aacaaatcga
     5401 tttagccgtt tggcgtcaat ggattccatt tcctgatgaa atcgatatca gacaaggcaa
     5461 aggtgcccta agaggttggt taacgctagc tgaaggtaca attactgact ggctaattga
     5521 taccgcttta catcaaatca gcgcaaacct aggcacaaac ttacccaaac tcgaactcga
     5581 tttcttgcaa ggacgtattg acgtcaagca atccaatcaa tccggaagct tagtccaaga
     5641 atggacggca caccaattaa atttcaaatt agcgcatcaa caaat
//
LOCUS       DAOQLJ010000139         5591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_6442_length_5591_cov_6.038837, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000139 DAOQLJ010000000
VERSION     DAOQLJ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6442_length_5591_cov_6.038837"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..586)
                     /locus_tag="PK522_09240"
                     /pseudo
     CDS             complement(23..586)
                     /locus_tag="PK522_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112925.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
     gene            complement(614..1813)
                     /gene="dapC"
                     /locus_tag="PK522_09245"
     CDS             complement(614..1813)
                     /gene="dapC"
                     /locus_tag="PK522_09245"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HNC41955.1"
                     /translation="MNPLLRNLQPYPFQKLSHLLEGITANASLAPINLHIGEPKHETP
                     AFILTELAKNLHGVANYPTTLGSKWLRTSIATWLVQRYQLTAIDPETEIIPVNGSREA
                     LFAFAQTVINSKTPEQPIVVCSNPFYQIYEGAALLAGAQPYFLNASAEQDFALNYEQL
                     PEAIWSRTQLIYVCSPNNPTGRVMTLDEWQHLFELSDRYGFVIASDECYSEIYFDEHT
                     PPLGALQAAQQLGRNKFTRLVVFSSLSKRSNVPGLRSGFVAGDAAILKDFLLYRTYHG
                     SAMNPAVQAASTIAWSDEQHVIENRRIYQEKFKDALAILNECMLAKMPDAGFYLWVKT
                     PINDRDFTQKLYRDYNVIVLPGSYLGREAHGFNPGENFVRIALVAPLAECSQAMQRIK
                     ACISQIH"
     gene            1946..2857
                     /locus_tag="PK522_09250"
     CDS             1946..2857
                     /locus_tag="PK522_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC41956.1"
                     /translation="MAGFMLTKQNNKLPILALLLGATCWGIAWYPYRLLNQSGFSGEA
                     ATALTYLVALISGIIFLRKNLSIQHNTASSENHWLIWIGITAGWTSIAYTLGVIHGEI
                     VRVLLLFYLAPFWTIFFARLLLNERLGLSGYWVIVLSLSGAMTILWQSGNDFPFPTQF
                     SDWMGLSGGIMFALNNVLIRKDQHHTIEQKSLAIWLGVFLLSFMLTGMTGTLPNFDQI
                     GNETGLILLGLGITIFLLSILLQYGLTHVPANQAIVILLLELFIAAIAAYYLADEQLS
                     TKEWIGGLMVVIASLLSTKIHYETSQA"
     gene            2928..3251
                     /locus_tag="PK522_09255"
     CDS             2928..3251
                     /locus_tag="PK522_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HNC41957.1"
                     /translation="MNKPLKNQKPISPRQRKYWSQHATESSNALDLELGIFTWKDPHK
                     IALSLKCSADNSQRRKANPFASAMSMLNFYINRAGTHLPVTQREILESAKEELRKLYG
                     RFPSA"
     gene            3313..3951
                     /locus_tag="PK522_09260"
     CDS             3313..3951
                     /locus_tag="PK522_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41958.1"
                     /translation="MSYVSLTHHQFDPNGFWEKPLQSVSVPAAQDLALFDQNGYDLTD
                     LEQHYAIINSAPAKSHREHHRALKTPWFTQPDRIEGAVLNHSLLFERKGYTGEALRQL
                     QQWAHTNPLIYKIIRIRPKWGLDFSMDYVDRSGNVFEVLHWEYDGFEYDEVAARKRLL
                     EVKFAAIDWDDAAINILRQKDQWHHLDFFAQSDWKCNYFGIVKERFKMVIWA"
     gene            3957..4337
                     /locus_tag="PK522_09265"
     CDS             3957..4337
                     /locus_tag="PK522_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNC41959.1"
                     /translation="MTKSTTTYWNPLISEHRSQWDAIKGLEGIAEEITLSIDSTTGEY
                     TRLTRFLPGADTWALGGKTHPYPEEIFIVSGRLYDQAFGRWLEAGDYASRPPGELHGP
                     FKTDIGCVVLEISFPNRIAEGNND"
     gene            4643..5137
                     /locus_tag="PK522_09270"
     CDS             4643..5137
                     /locus_tag="PK522_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC41960.1"
                     /translation="MFQETIKTCKAQNRVIVYIDESGFSHDMPRTHGYAPIGKRCHGV
                     CNWHARGRVNVIGALIGKCLLTVGLFISNIDADTFLGWTVHDLLPKLPPASVVVMDNA
                     TFHKRQDIQSVITQAGHTLEYLPAYSPDLNPIEYKWAQAKAIRKQQNQTVEQLFKIES
                     FYVA"
     gene            complement(5376..>5591)
                     /locus_tag="PK522_09275"
     CDS             complement(5376..>5591)
                     /locus_tag="PK522_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HNC41961.1"
                     /translation="TDASGTFNEVTRDAAWARMQSAGVQLMNWFGVACELHRDWRNDV
                     EGLGALFSSYLPNYRNLMTSYFTITKK"
BASE COUNT         1637 a         1241 c         1142 g         1571 t
ORIGIN      
        1 accatcattg aagataattg cttgatcggg caccaataaa gcaattatct tcaatgatgg
       61 tcgggtttgc ttgtaccggt tcaagtactc cgccaatccc aacgcctccc gataaatgga
      121 cattcttacc aatttgggca caagacccta ctgtcgccca agtatcaacc atcgttcctt
      181 catcgacata agcacctata ttgacataag aaggcatcaa caccacatta ttcgcaataa
      241 aagcaccttt ccgaacagct gcaggaggaa cgacacgaaa cccaccatcg cgaaaatcac
      301 gggaactata atcagcaaat tttgagggaa ccttatcaaa ataattcgag aatccccctt
      361 taataaaatt attatcctca atcctgaaag ataggagcac cgctttcttg atccattggt
      421 gcgttaccca ttcaccgcta atcttttctg caactcgtaa tttacctgca tcgagtaaat
      481 gaatgacttg agcgattgat tctttcagac ccgcctccac attacggggt gttaattcgg
      541 cacgccgttc aaacgctgct tcaatcgttg cttgcaactg ttccatgtta ttccttgaat
      601 gaatttaata attttaatga atttgggaaa tgcatgcttt tattcgctgc atcgcttgtg
      661 agcattcagc taatggagca acgagcgcaa tacgaacaaa attttctcct gggttgaatc
      721 catgggcttc acgcccaaga tagctccctg gcaaaacgat tacattataa tcgcgatata
      781 acttttgcgt gaaatcccga tcattgatag gcgttttgac ccataaatag aaacccgcat
      841 cgggcatctt tgccagcata cattcattta aaatcgcgag cgcatcttta aatttctcct
      901 gataaatccg gcggttctct atcacgtgct gttcatcgct ccaggcaatg gtactggctg
      961 cttgaactgc tggattcatg gcgctcccat gataagtccg atacagtaaa aaatctttca
     1021 aaattgctgc gtcccctgca acaaagccgg agcgtaagcc aggaacatta gagcgtttgg
     1081 ataaactgga aaaaacaacc aaccgcgtaa acttgttgcg tcctaattgt tgcgctgcct
     1141 gtagcgcacc taatggtggc gtgtgttcat cgaaataaat ttctgaatag cattcatccg
     1201 aagcaatcac gaacccatag cgatctgaca attcgaataa gtgttgccac tcgtccaaag
     1261 tcatcacccg tcccgttgga ttattagggg aacaaacata aattaattgc gtacgtgacc
     1321 aaatcgcctc tggtaactgt tcataattca gcgcaaaatc ttgctcagcg gatgcattga
     1381 gaaaataagg ttgcgcgcca gcaagtaatg ctgcaccttc ataaatttga taaaacggat
     1441 tagaacaaac tacgataggt tgttcaggtg ttttgctatt aatgacagtt tgagcaaatg
     1501 caaatagcgc ttcacggctg ccattgacag gaatgatctc agtttcagga tcaattgctg
     1561 tcaattggta acgttgtact agccatgtcg caatgctggt tcgtagccat tttgaaccca
     1621 gtgttgtagg ataatttgca acaccgtgaa gatttttggc caattcggtg agaataaatg
     1681 caggcgtttc atgcttgggt tcaccaatat gcagatttat cggcgccaaa gatgcattag
     1741 ctgtaatacc ctcaagtaag tgggacagtt tctggaacgg atagggttga agatttctca
     1801 ataacgggtt catttgtgat atgaattcta aaaccttgaa tttatgccat cattaacgat
     1861 taatattagt tgttcaggct acatgacttc tatttttcac tgcggtgaaa tttttttaac
     1921 tattaactat agaaccgcat tataaatggc tggattcatg ttaaccaaac aaaataataa
     1981 gctacctatc cttgcattat tattgggtgc tacttgctgg ggtattgctt ggtatcccta
     2041 tcgcttactc aatcaatccg gattcagtgg tgaagctgca actgctttaa cttatttagt
     2101 cgcattgatc tcgggtatta tttttcttcg taaaaattta tcgatccaac acaacacggc
     2161 ctcatctgaa aatcattggt tgatatggat tggcattact gccggatgga ctagtatcgc
     2221 ttatacactt ggtgtgattc atggcgaaat tgtaagagta ttactattgt tttatttagc
     2281 cccgttctgg acaatttttt ttgcgcgatt actcttaaat gagcgactcg gtttatcggg
     2341 ttattgggtc attgtccttt ccttgagcgg tgctatgacg atactttggc aatcgggtaa
     2401 tgattttcca ttccctactc aattcagtga ctggatgggt ctatcaggcg gtatcatgtt
     2461 tgcactcaac aatgtgctca tccgtaaaga tcaacaccat acgattgaac aaaaatccct
     2521 ggcaatatgg ctaggggtct tcttactgag cttcatgctc acgggtatga caggaacgct
     2581 acccaatttt gatcaaattg gtaacgaaac ggggttgatc ctactggggt taggcataac
     2641 catcttcttg ctcagtattt tgctacagta tggattaacc catgttcccg ctaatcaagc
     2701 aattgttatt ttgctgcttg aattgtttat cgccgcaatc gcagcttatt accttgccga
     2761 tgagcaatta agtactaagg aatggatcgg tggattgatg gtcgtaatag ctagtctact
     2821 ttccactaaa attcactatg aaacatccca ggcttaatta cggaaagtct tacttttttt
     2881 aatcaataga accatcgctt aaattcgtcg agttcgtatt tgaaaatatg aataaacctc
     2941 taaaaaacca aaagccaata tcacccaggc aacggaaata ctggtcacaa catgccaccg
     3001 aatcaagtaa tgcattagat cttgaactcg gtatatttac ctggaaagac ccacacaaaa
     3061 tcgcgttatc actaaaatgt tccgctgata atagccagcg gcgcaaagca aatccatttg
     3121 cttcagcgat gtccatgctc aatttttata tcaaccgagc aggaactcat ttaccagtaa
     3181 cgcagcggga aatattggaa agtgccaaag aagagctacg gaaattatat ggccgattcc
     3241 ccagcgctta gtagcacctt tttctattta taattaactt tatcaataat caatgagtaa
     3301 atttagtatt ccatgagcta cgtcagttta acccatcatc aatttgatcc taatggtttc
     3361 tgggaaaaac ctctccaaag cgtgtcagta ccagctgctc aagatttggc attattcgat
     3421 caaaatggct acgatctaac ggatttagaa cagcactacg cgataatcaa ctctgcacca
     3481 gccaaatccc accgcgaaca tcaccgggcg ttaaaaacac cgtggtttac acaacccgat
     3541 cgaatcgaag gtgcagtact caatcatagc ctactctttg aacgtaaagg ttataccggt
     3601 gaagcgttac gccaattaca gcagtgggca cacactaatc cgctcattta taaaattatc
     3661 cgtatccgac ccaaatgggg actcgatttc agcatggatt acgttgatcg ctctggcaat
     3721 gtctttgaag ttctgcattg ggaatatgat ggctttgaat acgatgaagt agccgctcgt
     3781 aaacggctat tagaagtcaa atttgcagca attgattggg atgatgctgc cattaatatt
     3841 ctgagacaga aagatcagtg gcaccactta gattttttcg cgcaaagcga ctggaaatgc
     3901 aattacttcg gaatcgtaaa agagcgtttc aaaatggtaa tttgggcata aaagctatga
     3961 caaaatcgac cacgacttat tggaatcccc tgatttctga gcatcgtagc caatgggacg
     4021 ccatcaaagg gctggaggga atcgccgaag aaattacgct tagtatagat tctactacgg
     4081 gcgaatacac tcgactcact cgctttctcc ctggcgctga tacatgggct ctgggaggaa
     4141 aaacacatcc ctatcctgag gaaatattta tcgtcagtgg ccggttatat gatcaagctt
     4201 ttggtcgctg gcttgaagcg ggagattatg caagccgtcc acccggggaa cttcatggtc
     4261 ctttcaaaac cgatattggg tgcgttgtgc tagaaatctc atttcccaat agaatagccg
     4321 aaggaaataa tgactgatcc agtttttact caaattgttc gcttgattta gaaaccaaac
     4381 taaaactgag ttgatatttg gtttgtcgtc gcaatgccta catttgctgt aatgtatatt
     4441 tttgcatata tctcgtcttt caatgttcct cttccatgcc gccagccacc tatagcaagg
     4501 ctcaaataaa gtgattatca agcagaacga gctcgtcgat taggcgtaag cgaaaaaggt
     4561 attggccacg cgttgcggcg tatgaacatc agctataaaa aaaacactgc ggcatcccaa
     4621 agcggacgaa gacaaacggc acatcttcca ggagacgatt aagacgtgca aagctcaaaa
     4681 tcgcgtcatc gtttatattg acgagagtgg tttttctcac gatatgccac gaacacatgg
     4741 gtatgcgccc attggcaagc ggtgtcacgg tgtgtgtaat tggcatgcca gaggccgcgt
     4801 caatgtgatc ggcgcgttga tcggcaaatg tcttctgacg gtggggttgt tcatcagcaa
     4861 tattgatgca gatacttttt tgggctggac ggtacacgat ctgttgccaa aacttccacc
     4921 agcatcagtt gtggtcatgg ataacgcgac ctttcacaaa aggcaggata ttcaaagtgt
     4981 aatcacacaa gctggacata cgcttgaata tctaccagcg tattcaccag accttaaccc
     5041 cattgagtac aaatgggcac aagccaaagc aatcagaaaa caacaaaacc aaaccgttga
     5101 gcagctcttt aaaattgaat cattttatgt ggcgtagcta tagcagcagc catagctcga
     5161 tgattctagc aatcaagtgt ggtgaattga ccattgcata aaatcagttg cgctttaaga
     5221 ttatccattg aagaccctcc agaagattag ccgcaagcgt gccagttttc aaaagtaaaa
     5281 aagatgcacc cacactgcct gaaaaataag acgccggtgc aaatttatct catgctcatt
     5341 catgaaaacc ggtctcccgt tataaatttg cttacttact tcttagtgat ggtgaagtaa
     5401 ctcgtcatta aattccgata gttaggtaga taactagaga acaaggcacc taaaccctcc
     5461 acatcgttac gccaatctcg atgcaattca caagccacgc caaaccagtt catcaattgt
     5521 acgccagccg attgcatacg cgcccaggct gcatcgcgtg ttacttcatt gaaagtaccg
     5581 gaggcatcgg t
//
LOCUS       DAOQLJ010000140         5572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_6481_length_5572_cov_6.309045, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000140 DAOQLJ010000000
VERSION     DAOQLJ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6481_length_5572_cov_6.309045"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..727)
                     /locus_tag="PK522_09280"
     CDS             complement(95..727)
                     /locus_tag="PK522_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC41962.1"
                     /translation="MKLLCHISFFLSIIALSFQAYSQQNSICGELKGPHYGPFDYLER
                     VQLRDKLEIVEAYHFSQNVEKLKGNVTSVAADLSYTLHAWPNHHRALASLAKLAIREK
                     AVNLPGLKYNVECYFDRAFRWRSNDSIVRSIYGSYLSKVGRANNAIEQLEIAVRLKSD
                     NATAHYNLGLLYFDKANYDKAQFHAESAYALDFPLPGLRNKLIRAGKWKK"
     gene            complement(790..2028)
                     /locus_tag="PK522_09285"
     CDS             complement(790..2028)
                     /locus_tag="PK522_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC41963.1"
                     /translation="MVRRVLLIAYHFPPLQGSSGIQRTLRFAQYLPKLGWEPIILTAN
                     PISYQLTRPTQLPKEFDNISIYRSFALDTARHLSLLKRYPRMLAVPDRWISWWLGAFP
                     QGLYLIKKFKPDVIWSTFPIATAHLIGYTLSRISGIPWAADFRDPMVQRDYPTDPIIY
                     RAYQWIEEKTIRNCASVIFTTANTRKDYISRFSTESESRFKVIENGYDEQLFSEVTVE
                     HQVKQNKQLTLVHSGIIYSSERDPTYFFEALSDLLKEKYISSDNLKIILRATYNDNFL
                     QQLINRFNIGSIVTLAPPISYKEAIAEMLSADGLIILQASNCNNQIPAKLYEYLRAQK
                     PILALTDPEGDTGLKLISLGFDTISRLDSKPDIKELILKFLDSIRNKIVQPLPLNQLQ
                     LHSRESRTSELVEILNGIVR"
     gene            complement(2022..2891)
                     /locus_tag="PK522_09290"
     CDS             complement(2022..2891)
                     /locus_tag="PK522_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 1, exosortase A system-associated"
                     /protein_id="HNC41964.1"
                     /translation="MNFSEQPLRFCCQGCWLYGILSIPFQSCKRGVLIVVGGPQYRVG
                     SHRQFTLLARFLADHDIPAFRFDYRGMGDSDGVIRTFENIGDDLESAIDQFFINVPSL
                     KEVVIWGLCDAASAALFYAHKDQRVTGLVLVNPWVRTESGVAKAYLKHYYTSRLLDGE
                     FWGKIIRGDFKLFDSFKFLFQSIKKSLIRKTEIANDKIKQSTCNLLLPLPQRMLEGLK
                     QYTGKIFIITSGDDLTAKEFLDLINTTKDWRQIINIKQVKFAHLKDANHTFSSRQWRD
                     QVAVWTTNWTKSW"
     gene            complement(2894..3730)
                     /locus_tag="PK522_09295"
     CDS             complement(2894..3730)
                     /locus_tag="PK522_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 2, exosortase A system-associated"
                     /protein_id="HNC41965.1"
                     /translation="MDSPTLCAEPFFLKVESGERFCLFYRPNLANPLGTFIFIHPFAE
                     EMNKSRRMIAQQSKAFSQNGFAVLQIDLYGCGDSSGEFNEARWEVWKQDIFHAINWIK
                     HQTAAPINLWGLRLGGLLALDLASGAEEFFQHIVLWNPIISGNTFLTQFLRLKVASQL
                     MIKSGKESVGTGALRNKIMAGETLEIAGYELSSILAIAIDKLRIGDFKVFDTKIHWFE
                     VVPEAGRVLSPSAMATVNSWKERNIDLDLQYVTGQAFWATQEIAECPELIEATINQFS
                     TV"
     gene            complement(3732..3989)
                     /locus_tag="PK522_09300"
     CDS             complement(3732..3989)
                     /locus_tag="PK522_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNC41966.1"
                     /translation="MQHIDEVRNILADTLSLGDRKHQLNENSFLLGSIPELDSMAVVN
                     VITALEDFYNISVDDDEISAKTFETLGSLTIFVEQKLSQSN"
     gene            complement(3994..4755)
                     /locus_tag="PK522_09305"
     CDS             complement(3994..4755)
                     /locus_tag="PK522_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC41967.1"
                     /translation="MMHFFKLLTIYKRIKEIVFQIGYINTAILLFHRILIKLFSGKLR
                     IYKYYLTAQPVTNRTFLPPNRGKKITVKQISRDDPIVSAFPRPDSIIKNRFDTGAVCL
                     VALKENVFVGYIWFMLGAYQEDEVRAKFVPLPASATVWDFDIYVHPDYRLGHTFLRLW
                     DEANRFLLDRKIEWSCSRISAFNAPSINVHTKLGALNLGQVIFFCIGSCQLTLATLFP
                     YIHVSTHLNSYPTFFIDTQKNNTIFPMKGKESTTE"
     gene            5153..5506
                     /locus_tag="PK522_09310"
     CDS             5153..5506
                     /locus_tag="PK522_09310"
                     /inference="COORDINATES: protein motif:HMM:NF025062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CZB domain-containing protein"
                     /protein_id="HNC41968.1"
                     /translation="MDLNQAIEKHAEWKVKFRSAIVRKESMDAGTISKDNCCELGKWL
                     HGIGRAKFSNLPSFKNCVLNHVRFHIEAGKVAQAINLKKYTEAETMLSNGTPYSLASN
                     EVGTAIIQLKKEAQI"
BASE COUNT         1794 a         1098 c          956 g         1724 t
ORIGIN      
        1 aattcgcatg aattcgaaca tttatagggc ccctaagcgt tttgcaggac cgacttttta
       61 taaggaaaag tgatgtacgt ccgccatgat cttcctactt cttccatttc ccagctctta
      121 taagtttatt tcgtaaacca ggcaaaggaa agtcaagcgc ataagcgctc tcagcatgaa
      181 attgtgcttt atcataatta gctttatcaa aataaagcaa tccaagattg tagtgcgctg
      241 tagcgttatc agactttaat ctcacggcaa tttcgagctg ttcaattgca ttattagctc
      301 taccgacttt tgataagtaa cttccataaa tcgatctaac aatactatca tttgatcgcc
      361 aacgaaaagc acgatcaaaa taacattcta cattgtactt caacccaggt aagttaacag
      421 ccttctctct aatcgcgagt tttgctaaag atgccaaggc tcgatggtgg ttcggccagg
      481 catgcaaggt atagcttaaa tctgctgcaa cactcgttac attacctttt agtttctcaa
      541 cattctgtga aaaatgatag gcctctacta tttcaagctt gtctcttaac tgaactcttt
      601 caagataatc aaaaggtcca tagtgtggac cctttaactc cccacaaata ctattttgct
      661 gagaataagc ttgaaatgat agggctataa tcgataagaa gaaactgata tggcataata
      721 atttcatata aatcgcctct tgagtaactt ctatatatca ttaattaatg ctaaaaaata
      781 aatcattggt caccttacga ttccattcaa aatttccact aactcagatg ttcttgattc
      841 tcttgagtgc agttgaagtt ggtttaacgg taaaggttga acaatcttat ttctaataga
      901 gtcaagaaac tttaatatta attccttgat atctggttta gagtctaacc gacttattgt
      961 atcaaaacct aagcttataa gttttagacc agtatcccct tctggatcag ttagtgccag
     1021 aatcggtttc tgcgcacgta aatactcata tagctttgca ggtatttgat tattacagtt
     1081 ggaagcctgc aaaataatta acccatctgc ggataacatt tctgcaattg cttctttgta
     1141 cgaaatcggt ggagctagcg taactatcga tccaatattg aatcgattaa tcagttgttg
     1201 taaaaaatta tcgttgtaag ttgctcgaag aataatcttt agattgtcag aagaaatata
     1261 cttttctttt agtaaatctg atagcgcctc aaaaaagtag gttggatcac gctcagacga
     1321 atagataatg ccgctatgaa caagcgttag ttgtttattt tgttttactt gatgttcaac
     1381 cgtaacttct gagaaaagtt gctcatcata accattttcg ataactttaa agcgcgattc
     1441 gctttcagta gaaaatcgac tgatgtaatc tttcctcgtg ttagctgtag taaaaattac
     1501 gctggcgcaa ttacgaattg ttttttcttc aatccattga taagcgcgat atatgatggg
     1561 atcagtagga taatcgcgtt gtaccattgg atcacgaaaa tcagccgccc acggaatacc
     1621 agatatccga ctcagtgtat aaccaataag atgcgctgtt gctatgggga atgttgacca
     1681 aatgacatct ggtttaaatt tttttatcaa ataaagtcct tggggaaacg ccccaagcca
     1741 ccaagaaatc catcgatcag gcacagcaag cattcttgga taacgcttta gcagtgataa
     1801 atgccttgcc gtatccagcg caaaagaccg atagatactg atattgtcaa attccttagg
     1861 taactgggta ggcctagtaa gctgatagga aattgggtta gcagttaaaa tgattggttc
     1921 ccaacctaat tttggaagat actgtgcaaa tcttaatgtt ctctgtatac cactgcttcc
     1981 ttgtaatgga ggaaaatgat acgcaatcag cagaacacgc cttaccatga ttttgtccaa
     2041 ttagttgtcc agaccgctac ttggtcgcgc cactgacgag aagaaaaggt atggtttgca
     2101 tccttcaaat gagcaaattt aacttgctta atattgatta tttggcgcca atcttttgta
     2161 gtattgatca agtctagaaa ttcttttgct gttagatcgt ctccgctggt aatgatgaat
     2221 atttttccgg tatattgctt tagaccctcc aacatccgct gcggtaatgg tagtaacaag
     2281 ttacaagtag attgcttaat tttatcattc gctatttctg ttttccttat taaggatttt
     2341 tttatagatt gaaataaaaa tttaaacgaa tcgaaaagct taaaatctcc ccttataatt
     2401 tttccccaga actccccatc tagcaacctc gaagtataat agtgtttaag ataggctttt
     2461 gcgacgccag attcagttct aacccaagga ttaactaata caagcccagt aaccctttga
     2521 tctttatgag catagaataa ggccgctgat gctgcgtcac acaaccccca aattaccact
     2581 tctttaagtg aaggtacgtt aataaagaat tgatcaatcg cactttccaa atcatcgcct
     2641 atattctcaa acgttcgaat tacaccatcg ctatccccca ttcctcgata atcaaagcga
     2701 aaagcaggaa tatcatgatc agcgagaaaa cgggcaagga gtgtaaattg gcgatgactt
     2761 ccaacccgat attgaggtcc tcccacaaca atcagtaccc cccttttaca tgattggaac
     2821 ggaatgctaa gaattccata caaccaacaa ccttgacaac aaaaacgcaa gggttgctca
     2881 ctaaaattca ttattaaaca gtcgaaaatt gatttatcgt ggcttcaatt aactcaggac
     2941 actcagcgat ttcttgagtt gcccaaaatg cctgtcctgt tacatattgc aaatccagat
     3001 cgatattgcg ctctttccag gaattgactg tcgccattgc tgaaggtgat agtactctgc
     3061 cagcttcggg cactacctca aaccagtgga ttttagtatc aaatactttg aaatcgccta
     3121 tccttaattt gtcgatagct attgcaagaa tcgaagaaag ctcataccca gctatctcaa
     3181 gtgtttcacc cgccattatt ttattacgta gagcacctgt cccaacactt tctttgccac
     3241 tttttatcat aagttggcta gctactttca acctcaagaa ttgtgttaga aaagtatttc
     3301 cactgataat aggattccac aaaacaatat gttggaaaaa ttcttcagcc ccactcgcta
     3361 aatcaagcgc aagtaaccca cccaaacgta gtccccaaag attaatcggg gcagctgttt
     3421 gatgtttaat ccaattgatt gcatgaaaaa tatcttgttt ccagacttcc caacgagcct
     3481 cattgaattc accactacta tcgccacatc catacaagtc aatttgaagc actgcaaaac
     3541 cattttgaga aaacgcctta gactgttgtg caatcatcct tcgtgattta ttcatctctt
     3601 ctgcgaaagg atgaataaaa ataaaggtac ccaatggatt agcaagattt ggtcgataaa
     3661 ataggcaaaa cctttcccct gattcaactt tcaaaaagaa cggctccgca caaagagttg
     3721 gagaatccat tctagttcga ctgtgacaat ttttgttcca caaaaatagt taagctacca
     3781 agggtctcaa aggtttttgc actaatttca tcatcatcaa ccgatatatt gtaaaaatct
     3841 tcaagtgcgg taattacatt tactactgcc attgaatcta attcaggaat gctgccaagc
     3901 aaaaaagaat tctcattgag ctggtgcttc ctatcaccga gactaagcgt atctgctaag
     3961 atatttctaa cttcatcaat atgctgcata aatttactcc gttgttgatt ctttcccttt
     4021 cataggaaaa atagtattgt ttttttgtgt atctatgaaa aaagttgggt atgagttaag
     4081 gtgtgttgaa acgtgaatat acgggaaaag tgttgctaac gttaattgac aactacctat
     4141 acaaaaaaag ataacttgcc ctaaatttaa tgcccctagt tttgtatgaa catttatcga
     4201 aggcgcatta aaggctgata ttctactaca cgaccattct atttttctat cgagcaagaa
     4261 tcgattagct tcatcccata aacgtaaaaa ggtatgccct aaacgatagt ctggatgaac
     4321 ataaatatcg aaatcccaaa cagtagccga ggcaggtaat ggaacaaatt ttgcccgtac
     4381 ttcatcttct tgataggctc ctaacataaa ccaaatgtag ccaacaaaaa cattttcttt
     4441 caaggcaact aggcatacag cgccagtatc aaagcgattt ttgattattg aatcaggtct
     4501 tggaaaagcg ctaacaatag gatcatcacg acttatttgc ttcaccgtaa tttttttacc
     4561 cctattgggt ggtagaaaag ttcgattagt aactggttgg gcagtcaaat aatatttgta
     4621 tatacgtagc ttaccactaa atagctttat gagaattcga tgaaacaaga gaattgctgt
     4681 attaatgtag cctatctgga aaacgatctc tttaatcctt ttataaattg ttaatagctt
     4741 aaagaaatgc atcatccacg cagctcatct tgtcataata tacttaaaaa attaaaagcc
     4801 gccctacgat ctagggtaaa aatattgcta aatctcattt tttatttatt aatcttgaat
     4861 acttatcgta tatcagtttt acttaattaa acaaaacaat aggcatgtga taaatccctt
     4921 ttttaacgtt agcctagtta aattttattt acccacaacg gcggaaggga ttcatatcta
     4981 tcatgaaaaa cattcattaa gccgatcctc atattattca ttttaaatcc ctaccgtttt
     5041 ctctaccaat tttaaagtgc tttacatgca atcttttaat aaacgaggcc atctctaaat
     5101 tttacagcaa ttactgtcgt aattagcttg attatttacc agtgagaata ttatggacct
     5161 aaatcaagcg attgaaaaac atgcagaatg gaaagttaaa tttagaagtg caattgtcag
     5221 aaaagagtct atggatgctg gaacaatttc aaaagataat tgctgtgaat tggggaaatg
     5281 gttgcatggt atcggtagag ctaagtttag taatttacca agtttcaaga actgtgtttt
     5341 gaatcatgtg agatttcata ttgaagcagg aaaggtagct caagctatca atttaaaaaa
     5401 atacactgaa gcagaaacga tgttaagcaa tggaacccca tacagcctag catcaaatga
     5461 ggttggaact gcaataattc aactcaagaa agaagcacaa atataggaaa aatgctctaa
     5521 aacacatcag agtgcgctat attgaattat gattagtttt ggttactcta ta
//
LOCUS       DAOQLJ010000141         5541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_6553_length_5541_cov_5.901021, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000141 DAOQLJ010000000
VERSION     DAOQLJ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6553_length_5541_cov_5.901021"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PK522_09315"
     CDS             complement(<1..792)
                     /locus_tag="PK522_09315"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNC41969.1"
                     /translation="MNDFWFKFPLHFIIYVALMLSPLSFAQEDISDRIEKKAKLSDTK
                     KNKKKKKKKNGIFFGNAGLGYRWDKGRSEIRLSGFMQIDTRSFFDRGDDWSNSDLLLR
                     RIQPSLDVKLNSTYSFHIMPSFASAPGILDIFIDGKFSDLINLRIGKFKSPFGLERLQ
                     SATALAFNERAFPTNLSPNREIGAQLFGELLNRTTEYQIGLFSGAVDNSSGLRDSVIY
                     SNHSGIDFVSRIFSHPFYHSEHSILNGLGMGIAYSYGTQQGHASGE"
     gene            complement(846..1697)
                     /locus_tag="PK522_09320"
     CDS             complement(846..1697)
                     /locus_tag="PK522_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNC41970.1"
                     /translation="MLVQSAIVMAEETTSCSGQLPRGVILSRNLPTDNQLKYYYYLPQ
                     HCTQQSNIFVTVHGISRNAEEHARKFAPYAEQYGVILIAPYFSENHFPDYQRLGRKGR
                     GKRADLALQEIIKDISKLAGINLPQKIYMFGYSGGAQFAHRYMLAYPKQVAKVVLGAP
                     GWYTFPDVSQKFPQGIQASPTLPYLTFNPSDFLKIPTCVLVGEFDKQRDNELNQSQKI
                     DRLQGKNRIERGQRWITEMTRQSRQLGLTTPYHFKLIPNSPHSFSLSMLKGGMGEKTF
                     AFLFGQN"
     gene            complement(1724..3433)
                     /locus_tag="PK522_09325"
     CDS             complement(1724..3433)
                     /locus_tag="PK522_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC41971.1"
                     /translation="MKIPVILKDQRRGLFIRLVINGLIQAGLTISTAWIVKEIFDRFL
                     LTQHLMLNFDVIGFGIALIVIAACIGWLRMVERLDAEQIGQNYIAETRILLYDYFCTL
                     APRSIQKRSRGTVLLRFVSDMTALKRWISLGLSRLTVAGVSTLTALFILAIINWQLTL
                     AVTLSLVTGGYIAYALGSRLRIAARETRRRLSYLAANLSDKVANIAVVQLFDQVGRER
                     ERIIRQNKQLHDSMIERARVAGQIHGITEFSAAVTSAIALFTGIIAVASGEATVGSVV
                     AAITVVGILMPPLRELGRVQEYWHGACVSREKLKTFLETPSFVTEISNAPALNLTSGR
                     LEFDSVTVSGGIKQLSGSIEPGQVVALVGPNGAGKSTLLQLAARLIDPDEGKILLDGQ
                     DLSQHSLKSIRRSIGMASPDLPLLRGSIEKNLRYRWRNVPEEEIIRVKTLCGIDEILA
                     QLPNGAKTKLSEGGTGLSTGQRQRIALARALLGNPLLLLLDEVDANLDAQASMIIDRI
                     IAEHHGTILLATHQLKHIKAADIVWYLEGGQLIEAGPPNKILYQNGPTVYFLGLHQQS
                     AAN"
     gene            complement(3456..4556)
                     /locus_tag="PK522_09330"
     CDS             complement(3456..4556)
                     /locus_tag="PK522_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HNC41972.1"
                     /translation="MSIQSIQLGRLKKAKLAYTPRNIALDKATSLLSGQDITPASGDL
                     VLAMVEKIGQHTSLELASGRRSTLFVGDEIIVCYGNRYAPDQFESEVPNDLLSCDLVA
                     SGGLASRMKSRHTNMKSPTKIYPIGLLTDNNGQRLNLSSIALPNLIPYAQRPYTIAVV
                     GTSMNAGKTTTAATLIRGLHNANYKVSAAKITGTGSGRDTWLMMDAGSAVTLDFTHAG
                     YPSTYLLTPEQINNVFATLTSQLSKDSPDVIVLEIADGLYQNETAKLLDSDMFAQSVD
                     SIIFAAGDAMGATSGVQWLTQKKLPIVAVSGRLTASPLAIQETVKATGLSVFNKETLS
                     SPAIANLLNIPRKKTQASSAVNWRLATMACNY"
     gene            5164..5427
                     /gene="rpsT"
                     /locus_tag="PK522_09335"
     CDS             5164..5427
                     /gene="rpsT"
                     /locus_tag="PK522_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNC41973.1"
                     /translation="MANTAQSKKRARQTIVRRGRNFRLRTRMRTAIKSVRKAIESGDK
                     ELAQSVFRESTKILDSIASKGIIHKNKAARHKSRLSHSIKVMA"
BASE COUNT         1602 a         1051 c         1237 g         1651 t
ORIGIN      
        1 ttctccactt gcatgacctt gctgagttcc ataactatag gcaattccca ttcccagacc
       61 atttaagatt gagtgttcgc tgtggtaaaa agggtgggag aatattcgac tcacgaaatc
      121 aatgccacta tgattggagt agatgacact gtcgcgtaat cctgaactat tatcaacagc
      181 gccactaaat aagccgattt gatattcagt ggtacggttg aggagttctc cgaaaagttg
      241 tgcgccgatt tctcgattag gtgatagatt ggttgggaaa gcgcgttcat tgaaggctaa
      301 tgcggtagca gattgaagac gctctagacc aaaaggcgat ttaaatttac cgatacggag
      361 gttaattaaa tctgagaatt tgccatcgat gaagatatct aagatgcctg gcgctgatgc
      421 aaaactaggc attatgtgga agctataagt gctatttagc ttgacatcga gtgaaggttg
      481 tatgcgacgt agcaataaat cgctattgct ccaatcatct ccacgatcaa agaaacttcg
      541 cgtatcgatt tgcatgaatc cgcttaggcg aatttcactt cttcccttat cccatcggta
      601 gcccaggcct gcatttccaa agaatatgcc gttttttttc ttctttttct ttttattctt
      661 ttttgtatcg cttaatttag cttttttctc gattcgatca gatatgtctt cttgagcaaa
      721 gcttaacgga gatagcatta aggcaacgta gataatgaaa tgaagcggga atttaaacca
      781 gaagtcgttc attttgaatt tgaatcaacc cttaggttat ttaaagtttg gctacgggca
      841 atgggttaat tttggccaaa tagaaatgca aatgtttttt cacccatccc accttttagc
      901 atgcttagcg agaaagagtg cggtgagttt gggattagct tgaaatgata aggtgtcgtc
      961 aatccaagct gacgcgattg tcgtgtcatt tcagtaatcc agcgttgtcc gcgctcaatg
     1021 cggttcttac cttgtaatcg atcgattttt tgtgactgat tgagttcatt gtcgcgttgt
     1081 ttatcgaatt ctccaactaa aacgcacgta ggaattttca agaaatcgga cggattaaaa
     1141 gtaaggtaag gcagtgtggg agaggcctgt atcccttggg ggaatttttg acttacatca
     1201 gggaaagtat accatccagg cgcacctagc acgacctttg ctacttgttt ggggtaagcg
     1261 agcatataac gatgggcaaa ttgtgcacca ccggaataac caaacatata gattttttgg
     1321 ggtagattga taccagcgag cttgcttata tccttgatga tttcttgaag agctaaatct
     1381 gctcttttgc cacgcccttt tcttcctaac cgttgataat ctggaaagtg gttttctgaa
     1441 aaataaggag caatcaagat gacgccatat tgttcggcat agggtgcaaa ttttcttgca
     1501 tgctcttctg cattgcgaga aataccatgg acagttacga aaatatttga ttgttgtgtg
     1561 cagtgctggg gaagataata ataatatttc agctgattat ccgttggtag gttgcgagac
     1621 agaataacgc cacgcggtag ctgacctgaa caggatgtag tttcttcagc cattacgatg
     1681 gcagattgca ctaacatgaa taagattccc caaaaaagta atttcaattg gcggctgact
     1741 gctgatgaag cccaagaaaa tatactgtcg ggccattctg gtacaaaatt ttgtttggag
     1801 gccctgcttc gattagctga ccaccttcaa gataccaaac gatatcggct gctttaatat
     1861 gtttgagttg gtgagttgcg agtagaattg ttccatggtg ctccgcaata attcgatcaa
     1921 tgatcatgct ggcctgcgcg tcaagatttg catcaacttc atctaataat agtagtaggg
     1981 ggttgcctag taatgctctg gcaagggcaa ttcgctgacg ttggcccgta gataaacctg
     2041 ttcccccctc agagagtttt gtttttgcgc cgttgggtaa ttgcgcaaga atttcatcga
     2101 taccacataa tgttttgact ctgataattt cttcttcggg cacattacgc cagcgatagc
     2161 gtaaattctt ttcaatagaa ccccttaata agggtagatc agggcttgcc atgccaattg
     2221 atcgacgaat tgattttagg ctatgttgtg ataaatcctg gccatcgagc agaatcttac
     2281 cttcatccgg atcaattaag cgggctgcca attgcaataa tgttgatttt ccggctccat
     2341 tcggcccaac aagtgcgaca acttgaccgg gttctattga accgcttaat tgctttattc
     2401 ctcctgaaac agtgaccgaa tcaaactcaa ggcgcccaga ggtaagatta agtgcgggcg
     2461 cattagaaat ttcggtcaca aaagaggggg tttctaagaa tgttttaagt ttctcgcggg
     2521 atacacaagc accatgccag tattcttgta ctcgacctaa ttctctgagg ggaggcatca
     2581 gaattccaac aacagtgata gcagcaacga ctgaaccaac agtagcttct cctgaagcaa
     2641 ctgcaatgat tcctgtaaat agtgcaatag ccgaagtcac tgctgctgag aattcagtaa
     2701 ttccatgaat ttgaccggct acacgtgcac gttcaatcat tgaatcatgc agttgtttat
     2761 tttgccgaat gatgcgttct cgttctcgac caacttgatc aaatagctga accacagcga
     2821 tattagcaac tttatctgat agattagctg ctaagtaaga taagcggcgt cttgtttcac
     2881 gtgccgctat tctaaggcga cttcctagtg cataggctat gtaaccgcct gttactagtg
     2941 aaagtgttac cgcaagggtt aattgccagt tgattattgc aagaatgaat aatgctgtaa
     3001 gagttgaaac gccagcgaca gtgaggcgtg atagcccgag actgatccag cgctttaagg
     3061 cagtcatatc actgacgaag cggagtaata cagtaccacg actccttttt tgtatagagc
     3121 ggggtgcaag tgtacagaaa tagtcataaa gtaaaatacg ggtttcagca atatagtttt
     3181 gccctatctg ctctgcatct aatcgttcta ccatgcgaag ccaacctata caggctgcga
     3241 tgacaatgag tgcaataccg aaaccgatca catcaaaatt tagcattaga tgctgggtta
     3301 acagaaagcg atcaaatatt tctttaacta tccaagcagt actgatagtt aatccggctt
     3361 gaataagtcc gttgatcacc agtcgtataa atagcccacg acgttgatct ttgagtatta
     3421 cagggatttt catctatcga gaattgaatg aataattaat agttgcaggc catggtagct
     3481 aagcgccaat taacagccga ggaagcttga gttttttttc gtggaatatt aagtaaatta
     3541 gcaatggcgg gggaactcag agtttcttta ttaaaaacag atagaccggt agcctttact
     3601 gtttcctgaa tcgctagcgg agaagccgtt aagcgtccgc tgactgcaac gattggtaat
     3661 tttttttgtg taagccattg aacgccggaa gtggctccca tcgcatctcc tgctgcaaaa
     3721 atgatgctat cgacagattg agcaaacata tctgaatcaa gtagctttgc cgtttcgttt
     3781 tgatataagc catcagcaat ttcaagaacg attacgtccg gactgtcttt acttaattga
     3841 ctggtaaggg ttgcaaatac attgtttatt tgttctgggg tcaaaagata agttgatgga
     3901 tatcctgcat gcgtaaaatc cagtgtaaca gcggagccag catccatcat cagccaagta
     3961 tctctacccg aaccagttcc agtgatttta gcagcgctga ctttgtagtt tgcgttatgt
     4021 agtccacgaa tgagggtggc ggctgtcgtt gttttgccag cattcattga agttccaacg
     4081 actgcaatgg tataaggccg ttgtgcataa gggattagat tgggtagtgc aatgctcgat
     4141 aaattgaggc gttgtccatt attatctgtt aataagccaa tagggtaaat tttagttgga
     4201 cttttcatat tggtatgacg gcttttcatg cgcgatgcta gtccgcctga tgcaactaga
     4261 tcgcaagaca acaaatcgtt aggtacttca gactcaaatt gatcgggagc ataacgattg
     4321 ccataacaaa cgataatttc atctcccaca aatagagttg agcggcggcc ggaggccagt
     4381 tcaagcgatg tgtgctggcc aatcttctct accatggcaa gtactaaatc acctgatgct
     4441 ggagtgatgt cttgaccgga tagtagagaa gtggctttat ctaatgcgat attgcgcggt
     4501 gtatacgcta attttgcttt tttaagacga cctaattgaa tggactgtat gctcatgggt
     4561 attgtcctct actcatatat aagattatgt gaactgttag aagcacttgc tcctcactta
     4621 acgatgctgg actagctcaa tatactggca tcattccagc taatttgatc agcttgaaat
     4681 ctgctatatg tgataggcac atctgagcaa aaactgataa ttaaattctg attcattttt
     4741 ttcaagctca tattttgtat ctcctagtgt tatcaggttg ctaaatcaaa tcgattattg
     4801 aagttttttg cgtgtgaaat tcagtacatg aaaacattat atgtgtaatt tattcccacg
     4861 taaatacatt tgtgtgattt ttttacatga tgttgctgcg gtggataaga tacgcgtaaa
     4921 aattgagatt aacttttttg tgtttcattg ttattaaaaa agcacgctat tggaccaacg
     4981 aaggtgaata atcaattaaa tgattctttg gtcattcaat tgattttgtg gctgcgtatt
     5041 gaaatgtacg aagatctagg aagagtaatt cagagaataa ggcttagcct tgcattctta
     5101 aacgaatgaa cgtaacattc agacttcgga gtatttgtag gtagaaaaaa gatttaattt
     5161 attatggcta atacagcaca atcaaagaag cgggcaagac aaacgatcgt aagaagaggt
     5221 cggaactttc gattgcggac aagaatgaga actgcaatta aaagtgttag aaaagcaatc
     5281 gaatcaggcg ataaggaatt ggcgcaatca gtttttagag agtcgacgaa aatattagac
     5341 tcgattgcga gtaaaggaat tatccataaa aacaaagcag cacggcataa aagtcgttta
     5401 tcgcattcaa taaaagtaat ggcctaataa acgtcttact tgatatatga atgccgatta
     5461 ttcgtgctag tttttctttt taaaacaaga aaaggaggag agaatagatg aagagcctgg
     5521 gatggaagag gggaatgatg c
//
LOCUS       DAOQLJ010000142         5401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_6846_length_5401_cov_5.479237, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000142 DAOQLJ010000000
VERSION     DAOQLJ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6846_length_5401_cov_5.479237"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="PK522_09340"
     CDS             <1..1483
                     /locus_tag="PK522_09340"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HNC41974.1"
                     /translation="GQSLFRYQITDVNSPWTDKIDRVGIYWGAPSDQPACGYDSDTKI
                     FLKTTGVNTKPLYFWDLRPERTTNKDQAVVVEGSISNLVQWLNNYTASTGKSLRHCAL
                     DFDPNRKNFLLWCGTADVWRITPPNPLSTFGWKAEMEPAIPSDQVPPLNVGTGILGKW
                     DYIPGFDVFMGLENETIGNIWVYKPIGWVTPDPDGNGGGGGGPINQLPNVTITSPVNN
                     QTISIGTPVTLSALASDSDGSIMEIKFFANNVFVDSLTTEPYSTGWIPPQAGEYIIKA
                     EAFDNLGAKVTSAPITINVVSDTPLTTTLLQRDSAGTIGTADTYLSAYHPSTNFGGSQ
                     NLYLSGKNYVPLIKFNIFSHEGGPIPDNAIIESATLHLYKGSSYSLLASLHAMNKPWN
                     EKEATWAKASSSLSWNALGAAAAGLDYVAIADAQVNLPWIADIWIALDVTQRVKTFSA
                     GSVNYGWRLLFNSGDSVNNIRFNSSEKSPELDKRPKLEIKWRI"
     gene            1758..3083
                     /gene="rimO"
                     /locus_tag="PK522_09345"
     CDS             1758..3083
                     /gene="rimO"
                     /locus_tag="PK522_09345"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNC41975.1"
                     /translation="MPTINKIPRVGFISLGCPKATVDSERILTRLRAEGYLISPSYQD
                     SDLVVVNTCGFIDSAIEESLDTIGEALRENGKVVVTGCLGAKGDTIKQVHPNVLSVTG
                     PQATEEVMEAIHIHLPKPHDPYVDLVPPQGIRLTPQHYAYLKISEGCNHRCSFCIIPS
                     MRGDLVSRSVGDIMQEAQNLVDAGVKELLVISQDTSAYGVDLKYRTGFWGGKPIKTRI
                     TELATALGELGVWVRLHYVYPYPHVDELIPLMSDLKIIPYLDIPFQHANARILKLMKR
                     PANSENVLARIQQWRTICPEITLRSTFIVGFPGETEQEFQELLDFLEIAQLDRVGAFM
                     YSPVEGAAANNLPDHISPEIQEERLARFMALQEKISTDKLKQKIGKTLEVLVDEVDSE
                     GAIARSHADAPEIDGLVYIDADQKTIQQGEFVKVRIVNSDQHDLWAELV"
     gene            3126..3578
                     /gene="sixA"
                     /locus_tag="PK522_09350"
     CDS             3126..3578
                     /gene="sixA"
                     /locus_tag="PK522_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HNC41976.1"
                     /translation="MELILWRHAEAEDGVPDASRRLTEKGMKQAQSMAQWLKPRLPKH
                     TRIIVSPAKRTQQTVAALCDTYETLEAVGTSTTADKVLATVNWPYEEGAVIVVGHQPT
                     LGQIAAAILGNPYASFSVKKGSIWWLSSKAKDLSSNGSLRLVMVPDMI"
     gene            3760..4950
                     /locus_tag="PK522_09355"
     CDS             3760..4950
                     /locus_tag="PK522_09355"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNC41977.1"
                     /translation="MDIDIVSALSGLIDLPWWGYIVVTLVLTHITIASITIFLHRHQA
                     HRALDLHPIPSHFFRFWLWLTSGMVTKEWAAIHRKHHAKCETLDDPHSPQIYGIKKVL
                     LEGSELYRKESKNLETLERYGHGTPDDWMERNVYTKHSVAGVASMLIINVVLFGPIGI
                     TIWAVQMLWTPIMAAGIINGVGHYWGYRNFRAEDASRNIVPWGILIGGEELHNNHHAY
                     PTSARLSNKWFEFDIGWMYIRILEMMGLAKVKKIAPKLNLNESKTVCDFDTLQAVIAH
                     RYEVLAKYTQSLKAILKNEMEQLLQVAAHHGVDGLTLKRWVLADASTLQDEERNKLSL
                     VLSKTKQLDKVYTMREELSAIWQRSSASKDELVKQLEDWCYRAEQSGIEALEKFSRRL
                     RCYA"
     gene            5167..>5401
                     /locus_tag="PK522_09360"
     CDS             5167..>5401
                     /locus_tag="PK522_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNC41978.1"
                     /translation="MNLIERAKDLVTRTGDRATANRVRTLSVLLAAKRALTHREIADQ
                     LLDAKKQLDRVTLYRILEWMSKKSLVHKIAGDDR"
BASE COUNT         1580 a          947 c         1244 g         1630 t
ORIGIN      
        1 gggacagtcg ctgtttcgct atcaaattac tgatgttaac tcgccttgga ctgataagat
       61 tgatcgagtc gggatttatt ggggtgcgcc ttcagatcaa ccggcatgcg ggtatgattc
      121 tgatactaaa attttcttga aaacaacagg tgttaataca aaacctttat atttctggga
      181 tttacgacca gaacgtacca caaataaaga tcaagcagtc gtggttgaag gttcaatttc
      241 caatctggtt caatggttaa ataattatac ggcttcaacc ggtaaatcac tccggcactg
      301 cgcacttgat tttgatccca atcgtaaaaa ctttttattg tggtgtggaa ctgccgatgt
      361 ttggcggata acaccaccaa atccgctctc tacatttggt tggaaagctg aaatggaacc
      421 cgctattccc tcagatcaag ttccgcctct taatgtgggg acaggaattc ttggaaaatg
      481 ggattatata cctggattcg atgtatttat ggggttagaa aatgaaacga ttgggaatat
      541 atgggtttat aaaccgatag ggtgggttac accagatccg gatggtaatg gcggcggtgg
      601 aggtggccca attaatcaat tgcccaacgt tactataact tcgcccgtta ataaccaaac
      661 aataagtatt ggaactccag taacgttatc tgcattggct agtgatagcg acggttctat
      721 tatggaaatt aaattttttg cgaataacgt atttgttgac tcgttgacaa ctgaacctta
      781 ttccacggga tggattcctc cacaagctgg agagtatatt atcaaagcag aagcatttga
      841 taatttgggc gcaaaagtaa caagtgctcc aatcacaatc aacgtagtat cagatactcc
      901 tttaactacg acattactac aacgtgattc tgctggaacg attggtactg ctgataccta
      961 tttatcggct taccatccta gcactaattt tgggggttct cagaatttat atttaagtgg
     1021 taaaaactat gttcctttga ttaagtttaa tattttctca catgaaggtg gcccgatacc
     1081 agataacgca atcatagaat ctgcaacgct gcatctgtac aagggatcat cttactctct
     1141 tttggcttcg ctgcatgcta tgaataaacc gtggaatgaa aaggaagcga cctgggccaa
     1201 agcttcaagt agcttatctt ggaatgcttt aggtgctgcg gctgcagggc tggattatgt
     1261 tgcaattgct gatgctcaag taaatttacc ttggattgca gatatttgga tcgcacttga
     1321 tgtaactcag agagtaaaaa cgttctcagc aggtagcgtt aattatgggt ggagattgct
     1381 gtttaattct ggtgattcgg ttaataacat tcgatttaat tcgagtgaaa agagtccaga
     1441 attagataaa cgtcctaagc tggagattaa atggcgtatt taattttatg atggctacta
     1501 cattatttag ttaacgagtt gtgttaattt tacaaagctc tagattggct gctgttaaag
     1561 gtcgatctag agtttagctt tttgataaaa tagttatttc atttactact ttagattctt
     1621 taatttattg aatactctgt gtcagttttt ttacttctag aatattaata cagagttcct
     1681 agagcgtatg tggccgtagt gtatgaatat attctgtggt tacctacata aaaggcaaca
     1741 atcgctaata aaaatatatg cctactataa ataaaattcc ccgcgtgggc tttatttcgc
     1801 ttggttgccc caaagcgact gtggattcgg aacgaatatt aacgcgtcta cgtgctgaag
     1861 gttatttgat ttcacccagt tatcaagatt ctgacctggt cgttgttaat acttgtggat
     1921 ttattgatag tgcaattgaa gaatcactgg atacgattgg agaagcgcta agggaaaatg
     1981 gtaaagtagt tgtcaccggt tgtttaggtg caaaaggaga tacaattaag caggtgcatc
     2041 ccaatgtgct ttcagttact ggcccacagg cgaccgaaga agtgatggaa gcaattcata
     2101 ttcatttacc aaaaccccat gatccttatg tcgatttggt tcccccgcag ggaattcggc
     2161 tgacccctca acattatgct tatttgaaga tttctgaagg ttgtaatcat cggtgctctt
     2221 tttgcattat tccaagtatg cggggagatc tcgtgagtcg atcggttgga gatatcatgc
     2281 aggaggcaca aaatttggtt gatgcagggg ttaaggaatt gctcgttatt tcacaagaca
     2341 ccagtgcata tggcgtggat ttgaaatacc gtaccggttt ctggggagga aaaccgatta
     2401 aaacacggat tacagagctt gcaactgcac taggtgagct cggtgtttgg gtgcggcttc
     2461 attatgttta cccttatccg catgtagatg aactaattcc attgatgagt gatcttaaga
     2521 tcattcctta tctcgatatc ccctttcagc atgcaaacgc ccgaattcta aagctgatga
     2581 aacgccccgc caatagtgaa aatgttctcg cacgaattca gcagtggcgg acgatttgtc
     2641 cagaaattac gctgcggagc acatttattg tcgggtttcc tggtgaaacg gagcaagagt
     2701 ttcaggaatt actggatttt cttgaaatcg cccagttgga tcgagttggc gcctttatgt
     2761 attctcccgt tgagggtgct gctgcaaata atttacctga tcatatctcg cctgaaattc
     2821 aagaagaacg cctggccaga ttcatggcac tacaagaaaa aattagcacg gataaattaa
     2881 aacaaaagat aggtaaaacg ctagaagtgc tagttgatga ggtggatagc gaaggcgcga
     2941 ttgcacggag tcatgctgat gcgcctgaga tcgatggatt agtatatatc gatgcggatc
     3001 aaaaaacgat acaacaaggc gagtttgtca aagtacgcat tgttaattcc gatcagcatg
     3061 atttgtgggc agagcttgtt taatagaatt ttaggcttta gtcgctttaa cttaggggaa
     3121 caagtttgga gttgatttta tggagacatg cggaggcaga ggatggagta ccagatgctt
     3181 cgcggagatt gactgaaaag ggtatgaaac aggcccaatc catggcccaa tggttaaaac
     3241 ctcgactgcc aaagcatact cgtatcattg ttagcccagc gaaacgaacc cagcaaacgg
     3301 ttgcagcgct atgcgataca tatgaaacac ttgaagcagt tggcacaagt accacagcag
     3361 ataaagtgct tgccactgtc aattggcctt atgaagaagg cgctgtaatt gtggtgggac
     3421 atcaaccgac attagggcaa attgcagcag caatattagg caacccttat gcaagcttta
     3481 gtgtaaagaa gggttcgatt tggtggctta gctcaaaagc taaggattta tcatcgaatg
     3541 gaagtctacg gttagttatg gtgcccgata tgatttagat taaaagtaag aagtaaaact
     3601 gttctatttc tgaatgtgtg aattttgtgt taattaagga aatgactgaa tttatgaggt
     3661 ttttttatta tttaaataat taagaaattt cttgactaac tggtcacatc tcattagatt
     3721 gattacttaa cataagccct agggaggata agttttaata tggatataga tatagtttcg
     3781 gctttatctg gattaatcga tttgccgtgg tgggggtaca ttgtcgttac attggtacta
     3841 acgcatatta ccattgcaag cattacaatt tttttacatc gccatcaagc acatcgtgct
     3901 ttggatttgc atccgatacc cagtcatttt ttccgtttct ggctttggct gacgagcgga
     3961 atggtaacca aagagtgggc agcgattcat cgtaagcatc atgcaaaatg tgaaacgtta
     4021 gatgaccctc acagtccaca aatttatgga attaagaagg ttttattaga gggttctgag
     4081 ctttatcgca aggaatcaaa gaacctagaa acactggaaa gatatggcca tggtacaccc
     4141 gatgactgga tggaacgtaa tgtatatacc aagcatagtg tcgctggcgt ggcttcaatg
     4201 ttaatcatta atgtcgtctt atttggacca attggaatca cgatatgggc agttcaaatg
     4261 ctttggactc ctataatggc agcaggcatt attaatggtg ttgggcatta ttgggggtat
     4321 cgtaattttc gtgctgaaga tgcgagccgt aatattgtac cgtggggaat tttgattggt
     4381 ggagaggagt tgcataataa tcatcatgct tatccgactt cagcacgact ttccaataaa
     4441 tggtttgaat ttgatattgg ttggatgtat atccgtattc ttgagatgat gggattagca
     4501 aaggttaaga aaattgcgcc taaattaaat ctcaatgaat ctaagacagt atgtgatttt
     4561 gatacattac aagctgttat tgcacatcgt tatgaagtat tggctaagta tacgcaatca
     4621 ctgaaagcaa tccttaaaaa cgagatggaa caattgttgc aagttgcagc gcatcatggt
     4681 gttgatggct taacattgaa acggtgggta ctagcggacg ctagcacttt gcaagatgag
     4741 gagcgtaata agctgagttt ggtgctgagt aaaacgaagc aacttgataa agtttatacg
     4801 atgcgtgaag agttatctgc aatctggcaa agatcatctg cttctaagga tgagctagtg
     4861 aagcagctag aagattggtg ctatagagca gagcaaagcg gtattgaggc acttgagaaa
     4921 ttctcgcgta gacttcgctg ttacgcataa agtttgtatc tttgctctcg agcaaattag
     4981 cttcgtgtaa tttgctcgag atgtcctaaa gctagattgt tactattttt ttagctccac
     5041 gtaagtcaca catcttttcc cagttccgag tattccttaa tttttgtatt gtttcatcaa
     5101 agtaaagata gtaaaaattg attttcttac gctataatag tgcaactgtg ttgcattaat
     5161 tgcattatga atttaattga aagagcaaaa gatttagtaa ctcgtacagg agatcgagcg
     5221 actgccaatc gagttagaac actttccgtt ttgctggctg cgaagcgggc actaactcat
     5281 cgtgaaattg cagatcaact attggacgca aagaaacaat tggatcgtgt aacgctttat
     5341 cgcatcttgg agtggatgag taaaaagagt ttggttcata agattgcagg cgacgacaga
     5401 g
//
LOCUS       DAOQLJ010000143         5398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_6851_length_5398_cov_6.704099, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000143 DAOQLJ010000000
VERSION     DAOQLJ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6851_length_5398_cov_6.704099"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /gene="ybgF"
                     /locus_tag="PK522_09365"
     CDS             complement(<1..735)
                     /gene="ybgF"
                     /locus_tag="PK522_09365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNC41979.1"
                     /translation="MVLRASSFLVVALLLVSKPSIAGLFGDEKAREQIDLLQKQTQAM
                     EARIAGMEELLKSNALIELYSQVEGFKIELGKLTGQIEVLTEENKLLRKQQKDFYLDL
                     DNRLNQVESEGSRESGNTSLGEPDSSTALPSEIEMTAQTVDGSEREAYQTGYNLFREG
                     DYLAAISHFEQFLKQYASSSLAPGAAYWIGNAHYALRNFQEAISAQQRVIDQYPKSAK
                     APDALLNIASSQLEIPDVSAAKKTLEN"
     gene            complement(735..1247)
                     /gene="pal"
                     /locus_tag="PK522_09370"
     CDS             complement(735..1247)
                     /gene="pal"
                     /locus_tag="PK522_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HNC41980.1"
                     /translation="MKKLLTVLIICLLSACASQNKQSAEVDDRAMAGGGSGSQDGAGA
                     GGFGLSPLRDPNNILSQRSVYYDYDSYTIKSEYRELVLAHARYLRDNGDAKVLLQGNT
                     DDRGSREYNLALGQKRANSVKNAMLLSGARDQQIEAVSLGEEKPRATGHGESAWAENR
                     RADILYQGEY"
     gene            complement(1265..2575)
                     /gene="tolB"
                     /locus_tag="PK522_09375"
     CDS             complement(1265..2575)
                     /gene="tolB"
                     /locus_tag="PK522_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HNC41981.1"
                     /translation="MRVFTRYCYLALSLLLWSAQVHATLNIEIFGGGSKKIPVAIVPF
                     SQEERLPQSITAIVSADLERTGLFKMVGTFGQTPHEPSDIVYADWQSRGASAIVIGST
                     HLLPDGRVDVRFRLMDVAKTSQITGFTGVVPSDQLRVAAHRIADIIYEALTGDMGVFS
                     TRIAFVAKTGNKFALQIADSDGYNAHSIIEYSEPIISPAWSPDGSKIAYVSFENKKPV
                     IYVQTLATRERKAVANFKGSNSAPAWSPNGDKLAVVLTLHGGSQIFLINADGSGLQRL
                     AQSPGIDTEPNFSPDGRSIIFTSDRGGSPQIYRMAISGGNAGVAERLTFEGSYNVSPR
                     FSPDGKSFTYIHRNGDRFNIALQGLANRQVQLLTDSKFDESPSFAPNGKMILYATEIS
                     GRGILSVVSSDGQTKQRLSSQTGDIREPSWGPLLKSQRNNHFIN"
     gene            complement(2611..3465)
                     /gene="tolA"
                     /locus_tag="PK522_09380"
     CDS             complement(2611..3465)
                     /gene="tolA"
                     /locus_tag="PK522_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HNC41982.1"
                     /translation="MTTLAWRRASDEEPGLIRSAALALLVHIVFFGFMVFGLNWRNDP
                     NESMVVDLWRDMPQQPIKPQPVIKEPIKELPKPIPPAQEPVKPQPETVQPKSEPVKIK
                     NPPPRKPVIEIKEKIEPPKPIQEEKKVEPVQEKVPKPEPVKEQVKKPEPIKEVEKKVE
                     PEKELPEKEQKTVEQDVLAQLAAQKSKALDEIAKYKAMIQAKIRSRIVTPPDLPGNPM
                     AEFRVTLLPGGDVLKVSLRKSSGYAAFDEAVERAIFLAKPLPIPPDPALFKEFRDFSL
                     TVYYQDAL"
     gene            complement(3474..3890)
                     /gene="tolR"
                     /locus_tag="PK522_09385"
     CDS             complement(3474..3890)
                     /gene="tolR"
                     /locus_tag="PK522_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HNC41983.1"
                     /translation="MANLRNKRRLMNDINVVPYIDVMLVLLIIFMITAPLIPPGQVEL
                     PSIGKSSSPPVEPLEVIIAVDGSLTLRDRARAGDEQKVNRTQLVAIIKNKQAQNTEQP
                     VVIAADKNVRYEEVINIMDVLEQEQVKKVGLLIKPK"
     gene            complement(3906..4589)
                     /gene="tolQ"
                     /locus_tag="PK522_09390"
     CDS             complement(3906..4589)
                     /gene="tolQ"
                     /locus_tag="PK522_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HNC41984.1"
                     /translation="MSATVTQDMSLLHLVTSASLPVQLVMLMLLIASLLSWWFIFRKL
                     FAIREAIKQSDEFENTFWRGVDLNALYQRAAGARDRAGSMERIFEAGFREFTKHQSGM
                     DIGAIMDSTRGAMRAAYQREMDRLEAHLSFLATVGSVSPYVGLFGTVWGIMNSFRELS
                     NLTQATIAHVAPGIAEALIATAMGLFAAIPAVIAYNRYASDTSQLATRYESFMEELSN
                     VLQRRAVKL"
     gene            complement(4596..5021)
                     /gene="ybgC"
                     /locus_tag="PK522_09395"
     CDS             complement(4596..5021)
                     /gene="ybgC"
                     /locus_tag="PK522_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HNC41985.1"
                     /translation="MSSLPSSPEDKAFSHPVRVYYQDTDTGGVVYHASYLNFLERARY
                     EWLRDIGFSVDSLIKEHKTIFLVRSLNIEYFKPAVLDDLLRVTVEVVDMSRSRITLSQ
                     QVIRDQINLVSATIHVVCVSVDNLRPIRIPTPLFQRINS"
     gene            complement(5070..>5398)
                     /locus_tag="PK522_09400"
     CDS             complement(5070..>5398)
                     /locus_tag="PK522_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HNC41986.1"
                     /translation="EIKAQGHVNQAIADAALKMLDIDEVGLDILDRKFLLAILEKFEG
                     GPVGIDNLAAAINEERDTIEDVIEPYLIQQGYLKRTPRGRVATDLTYQHFKLLPLRDS
                     PNGDLW"
BASE COUNT         1504 a         1289 c         1018 g         1587 t
ORIGIN      
        1 gttttctaag gtttttttag cagccgatac gtcaggaatt tccaattgac tactagcaat
       61 attgagaagt gcgtcaggcg ccttcgcgct cttaggatat tgatcgatta cacgttgctg
      121 agcagaaata gcttcttgaa agttacgcag tgcgtagtgg gcatttccta tccagtaagc
      181 cgctcctggc gcaagactag aacttgcata ttgtttgaga aattgctcaa aatgactgat
      241 cgctgctaaa taatcccctt ctcggaataa attatatcca gtttggtaag cttcgcgttc
      301 cgagccatcg actgtttgtg cagtcatttc aatttcggat ggcaatgcag ttgatgaatc
      361 aggttcaccc agcgatgtat tccctgattc tcttgatcct tccgactcaa cctgattcaa
      421 gcgattatcc aaatcgagat agaaatcttt ctgctgttta cgaagtaact tgttttcttc
      481 agtcaatacc tcgatctggc cagtcaattt tcctagctct attttaaaac cttccacttg
      541 agaataaagc tcaattaatg cattactttt gagcaattcc tccattcccg caatgcgcgc
      601 ttccattgct tgtgtttgtt tctgcaaaag atcgatctgc tcacgcgctt tttcatcgcc
      661 aaaaagccca gcaatactgg gcttactaac caataaaagc gcaacaacca gaaaagaaga
      721 agcgcgcaat accattaata ctctccttgg tagagaatat ccgctctacg attctcagcc
      781 caagcagatt ctccatgtcc tgttgcacgg ggtttctctt cacccaaact tactgcttct
      841 atttgttgat ctcgtgctcc agataacaac atcgcgttct tcacgctatt agcacgtttt
      901 tgccccagcg caagattata ttcacgacta ccccgatcat cggtattccc ttgcaataag
      961 acttttgcat cgccattatc acggagataa cgtgcatgtg ctaacacgag ctctctatat
     1021 tcgcttttta tggtatagct atcatagtca taataaacac ttcgctgaga tagaatatta
     1081 ttaggatcac gcaaagggct taaaccaaat ccacctgcac ctgctccatc ttgacttccg
     1141 gaaccaccac ctgccattgc acgatcatca acttcagctg actgtttatt ttgacttgca
     1201 catgccgaca acaagcagat aatcagcact gttaataatt ttttcattgc ttatcctttt
     1261 caaattagtt aatgaagtga ttatttcgct gtgatttcag caaaggaccc cacgacggtt
     1321 ctctaatatc acccgtttga gatgaaagcc gttgtttcgt ttgtccatcg ctggaaacga
     1381 cggataatat accacgtcca cttatttctg tagcatacaa aatcattttt ccgttgggag
     1441 cgaagctggg agattcatca aacttgctat ccgtcaataa ttgcacttgg cggttggcaa
     1501 gcccttgcag ggcaatatta aaccgatcac cattgcgatg aatataagta aaactttttc
     1561 catccgggct aaagcgtgga cttacattat aacttccttc gaatgtaagt cgttctgcaa
     1621 caccggcgtt accacctgaa atagccattc gatagatttg cggacttcca ccgcgatccg
     1681 aagtaaaaat aatcgaccgc ccatcgggtg agaaattggg ctcggtatca ataccgggac
     1741 tttgggctaa tcgttgtaga ccgctaccat ctgcattaat taaaaatatc tgcgaaccac
     1801 cgtgtaacgt taatacaacc gctaacttgt caccattcgg tgaccaagca ggagcactat
     1861 tacttccttt aaaatttgca acggcctttc tttcacgtgt tgccagcgtt tgcacataaa
     1921 taaccggctt cttattttca aaagagacat aagcaatctt gctcccatca ggtgaccatg
     1981 cgggtgaaat gattggctca gaatattcaa tgatcgaatg cgcgttatat ccatcagaat
     2041 cagcaatctg taacgcgaat ttgttacctg ttttggctac aaatgcaatg cgggtgctaa
     2101 agacgcccat atctcctgtc aaagcctcat aaataatatc cgcaattcgg tgggcagcta
     2161 cgcgtaattg atccgacggc acaacgccgg tgaatcctgt aatctgggat gtttttgcca
     2221 catccattaa acggaagcgt acatcgactc gcccatctgg caatagatga gtacttccaa
     2281 taacaatcgc acttgcccct ctactctgcc agtctgcata gacaatgtcg ctcggctcat
     2341 gcggagtttg gccaaaagtg cctaccattt taaatagacc cgttcgctca agatcggctg
     2401 atacaatagc cgtaatgctc tgaggaagtc tttcttcttg agaaaaagga acaattgcga
     2461 caggaatttt tttcgaacca cctccaaata tttcaatgtt tagtgtggca tgaacctgag
     2521 cactccatag caataaactg agtgctaaat aacaatagcg tgtaaatact cgcatataaa
     2581 ctctcaacaa attaattaat cgactcgctg ttataaagca tcttgatagt ataccgtgag
     2641 actaaaatca cgaaattcct taaacaaagc tggatcaggc ggaataggta gcggttttgc
     2701 taaaaaaata gccctttcaa ccgcctcatc aaacgctgca taaccactgc tttttcgtaa
     2761 cgaaaccttg agcacatcac ccccaggtaa caatgtcact cgaaattcgg ccatgggatt
     2821 accgggaaga tcaggcggtg tgacaattct gcttctaatc tttgcctgta tcatcgcctt
     2881 atattttgca atctcatcta aggctttact tttttgtgca gccaactgag ccagtacatc
     2941 ttgctctacc gttttctgct ctttttcagg taactctttc tctggctcta ccttcttttc
     3001 cacttcttta atcggctctg gctttttgac ttgctcttta accggttcag gtttgggtac
     3061 cttttcttga accggctcta cctttttctc ttcttgaatg ggcttagggg gctcaatttt
     3121 ttccttgatt tcaatcacag gtttccgcgg tggtggattt ttgattttta ccggctccga
     3181 tttcggttga acggtttctg gttgaggttt aacaggctcc tgagctggtg ggattggttt
     3241 agggagctct ttaatcggct ccttaatcac aggctgaggt ttaatgggct gttgtggcat
     3301 gtctctccat aaatccacaa ccatactttc attgggatca tttctccagt tgaggccaaa
     3361 caccatgaaa ccaaaaaaaa cgatatgtac caataatgcc agtgcagctg accgaatcaa
     3421 ccctggctct tcatcggaag ctcttctcca agctaatgtc gtcattgtta cttttatttt
     3481 ggcttgatca acaagcctac ttttttaacc tgttcttgct ccaaaacatc cataatatta
     3541 attacttctt cataccggac attcttatca gcagcaatta caacaggctg ttcagtattt
     3601 tgggcctgct tatttttaat gattgcgacc agctgtgtgc gattcacttt ttgttcatca
     3661 cccgcacgtg ctcgatcacg taacgttaaa ctgccgtcta ctgcaataat aacttccaat
     3721 ggctctaccg gtggactcga tgatttacca atggaaggta attcaacctg acccggtggg
     3781 ataaggggtg ctgtaatcat aaaaatgatg agcaaaacca gcatcacatc aatgtaagga
     3841 actacattga tgtcattcat taaacgacgt ttattacgaa gattagccat tagcttctta
     3901 gaatcctaaa gttttacagc gcgtcgctgc aatacattcg atagctcttc cataaagctc
     3961 tcataacgtg tagctagctg actagtatca ctagcatatc gattataagc aatgacagca
     4021 ggtattgccg caaataatcc catcgcagtc gcaatgagtg cttctgcaat gccaggcgca
     4081 acatgggcaa tggtcgcctg cgttaaattc gataactcac gaaatgaatt catgataccc
     4141 cacactgttc caaataatcc aacataagga ctcaccgacc caacagtcgc cagaaaagat
     4201 aaatgtgctt caagtcgatc catctcacgc tgatacgctg ccctcattgc acctcgggtg
     4261 ctatccataa ttgcaccaat atccattcct gattgatgtt tagtgaattc acgaaatcct
     4321 gcttcaaaaa tacgttccat actaccggct ctgtctcttg ccccagcagc acgctgatag
     4381 agcgcgttta agtcgacgcc tctccagaac gtattttcaa attcatcact ttgcttaatg
     4441 gcctccctga tcgcaaacaa ttttctgaaa ataaaccacc aggaaagtaa agatgcgatc
     4501 aacagcatca acatgaccaa ttggacggga aggctagcac ttgttacaag atgtaacagt
     4561 gacatatctt gggttacagt tgcgctcata aaaatttaac tattaattcg ttgaaaaaga
     4621 ggtgttggaa tacggattgg ccttaagtta tctacactaa cacataccac gtgaatcgtg
     4681 gcgctcacca agttaatttg gtcacgtatg acttgctgag ataatgtgat acgacttcta
     4741 ctcatatcaa ccacctctac agtgacccgc aataaatcat caagcacagc cggtttgaaa
     4801 tattctatat taagcgatcg aactaaaaag attgttttat gttctttaat cagggaatca
     4861 acactaaagc cgatatctcg caaccattca tagcgggcac gctccaaaaa attgagatag
     4921 gaagcatggt atacaacgcc ccctgtatcg gtatcctgat aataaacacg gacaggatgt
     4981 gaaaatgcct tatcttcagg ggaagatggc aaagagctca ttgtattcat ttcaaaaaaa
     5041 cgataggaag caatttcttc ttcaaattat caccacaaat ccccattggg gctatctcta
     5101 agcggtagta gcttaaaatg ttgataggtt aagtctgttg caacacgccc tcgtggtgtt
     5161 cgcttgagat aaccttgctg aattaagtaa ggttcaatca catcttcaat cgtgtcacgt
     5221 tcctcgttaa tggctgcagc aaggttatcg attccaactg gaccaccctc aaatttctct
     5281 aaaatggcca gtaaaaactt tctgtcgaga atatccaagc ctacttcatc gatatccaac
     5341 attttcaagg ctgcgtcagc aatagcttga ttgacatgac cttgcgcctt gatttcag
//
LOCUS       DAOQLJ010000144         5380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_6894_length_5380_cov_6.550423, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000144 DAOQLJ010000000
VERSION     DAOQLJ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6894_length_5380_cov_6.550423"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PK522_09405"
     CDS             complement(<1..1061)
                     /locus_tag="PK522_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNC41987.1"
                     /translation="MSETKSIHLLINWCKPYRVFFWLVAAILLIVGVSSATLPLVAKA
                     LLDHMLDQDQETMMVTMFTLICIISVRTIANYFADFILRWIAGKLSTDLQTLLLEKAL
                     ALPVYHFEQHDLKQFYIDHIANILKIADLSTRVFWGQVKEILIFTGLVAIMFYINRDA
                     AVLGIFLILVTVLIAQILNKGPNHRNSQSLAITKSSDLFQQIMKHIRSIKIDESLRQE
                     RNNFYQRLERQRISLLKPIMGNFLGKLLAIIVFSLMLIAVFYYLLHQYHLHKLVLSEM
                     IAMIGAFGLLAPASARFLNRIAEMDTGRIALQQLNEFLSLQTQPPKEGIALGTIQGAL
                     VIENLKPQGRDSLDDSSFH"
     gene            1212..2135
                     /gene="rfbA"
                     /locus_tag="PK522_09410"
     CDS             1212..2135
                     /gene="rfbA"
                     /locus_tag="PK522_09410"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNC41988.1"
                     /translation="MNTHPSEPTQQRPSTPRKGIILAGGSGTRLYPVTQVISKQLLPI
                     FDKPMVYYPLSTLMLAGIRDILIISTPQDTPLFKQLLENGEPWGLNLSYAVQPSPDGL
                     AQAFIIGESFLGNDYSALILGDNIFYGHDFHHLLDNATQRATGATVFAYHVHDPERYG
                     VVGFDSAGRAINLEEKPQQPQSNYAVTGLYFYDQQVVEYAKSLKPSARNELEITDLNR
                     IYLEQNKLHVEIMGRGYAWLDTGTYESLLDASKFVATIEHRQGLKIACPEEIAFRKGW
                     ITAEQLDKLARPLAKNSYGKYLQQILNQPLL"
     gene            2163..2708
                     /gene="rfbC"
                     /locus_tag="PK522_09415"
     CDS             2163..2708
                     /gene="rfbC"
                     /locus_tag="PK522_09415"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNC41989.1"
                     /translation="MEAIQLAIPDVYLIQPRVFGDERGFFFESYNQQQFNAAINRPIS
                     FVQANHSRSARHVLRGLHYQIKQPQGKLVRVVSGEVFDVAVDLRKHSATFGQWVGEIL
                     SAENKKQLWIPEGFAHGFLVLSESADFLYQTTDYWAPAFERCILWNDPVLNIQWPIDQ
                     SPVLSGKDAQGTSFKLAETYP"
     gene            2753..4102
                     /gene="radA"
                     /locus_tag="PK522_09420"
     CDS             2753..4102
                     /gene="radA"
                     /locus_tag="PK522_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNC41990.1"
                     /translation="MAKSKSLYFCNACGGQSLKWQGQCPHCREWNTLVEGVAEKKGPS
                     LISGLRPTEIQSLSDIEVTETPRLSTTIGEFDRVLGGGLVLGGVVLLGGDPGIGKSTL
                     LLQALSAMSMDHRVLYVSGEESAQQIALRARRLNLDVSKVSLLTEIHLENIHAMLSNA
                     APAITVIDSIQTIYSDTLQSAPGSVAQVRECTAQLTRIAKSKGICMILIGHVTKEGSL
                     AGPRVLEHMVDTVMYFEGDAHSSFRLIRSFKNRFGAVNELGVFAMTEKGLRGVNNPSA
                     FFLSHHHKPVPGSCVMVTQEGTRPLLVEIQALVDDTHTANPKRLSIGLEQNRLAMLLA
                     VLHRHAGIPCFNHDVFINAVGGIRITEPGADLAVLAAIVSSIKDKPLAEKMVVFGEIG
                     LAGEIRPVQRGQERLKEAAKLGFIKAIIPESNKPKQPIPDIEILTVNRVEQALALLR"
     gene            4099..4914
                     /locus_tag="PK522_09425"
     CDS             4099..4914
                     /locus_tag="PK522_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phytoceramidase"
                     /protein_id="HNC41991.1"
                     /translation="MKSVTTSRKIRIYLLILLTCALLLAAAVLPPVSQSTLYHEFADQ
                     RSYWGIPNFLNVVSNLIFLIVGIAGLIYLRRESLLQQNQPLSAEIKSYRLVFASTAAA
                     CLGSIYYHWEPSLISLFWDRLPIAIGFMSLLAATVMERFATNWVTTYSVILLPIAGAM
                     STLYWLWSELYSVGNLNFYLAAQFCSIILILLLVSSFPSRYSHASEIYRILSIYGLAK
                     LAEIADQEVYQFGNIISGHTLKHILAGLAVYFIMRMLKIRTLKPNHEIYVSKK"
     gene            complement(4964..>5380)
                     /locus_tag="PK522_09430"
     CDS             complement(4964..>5380)
                     /locus_tag="PK522_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41992.1"
                     /translation="GASGVAVSFATGRDSISLRKIERFTGQTIAPFVVPGMESKYKPK
                     TGGGRSHSGRDQRMPNITHKRNRSWGGGNQLTEERKTQNHSNQSKRDNHTFNDFSAGS
                     EARKRPVRNRSGFSSGFNSFSGSGRSNQSRTPRFGS"
BASE COUNT         1520 a         1212 c         1120 g         1528 t
ORIGIN      
        1 ctatggaatg atgaatcatc aagcgaatca cgaccttgcg gcttaagatt ctcaataacg
       61 agcgcgcctt gaatcgttcc aagggcgatt ccttctttag gaggctgtgt ctgcaatgat
      121 aggaattcat tgagctgttg caacgcaatc cgtcctgtat ccatctctgc aatacgattc
      181 aaaaaccgag ctgaagcagg cgcaagtaat ccgaatgcac caatcattgc aatcatttca
      241 ctgagcacta gcttatgaag atgatattga tgcaacaagt aataaaaaac tgcaatgagc
      301 attagagaaa agacaatgat cgccaggagc ttacctaaga aattgcccat aatcggtttc
      361 agtaaggata tccgctgtct ctcaagtcgt tgataaaaat tgtttctttc ttggcgtaaa
      421 ctctcatcaa ttttgatcga tcgaatatgc ttcataattt gttgaaataa atccgacgat
      481 tttgtgattg ccaaggattg actattacga tgattgggtc ctttattaag gatctgagcg
      541 attaaaacag taaccaaaat caaaaagatt cctagcaccg cggcatcccg attgatataa
      601 aacatgatgg cgactaagcc agtaaaaatg aggatttctt taacttgacc ccaaaatacg
      661 cgcgtactga gatctgcaat tttcaatata tttgcaatgt gatctatgta aaattgcttg
      721 aggtcatgct gctcaaagtg gtaaaccggt aatgctagcg ctttttctaa tagaagcgtt
      781 tgaagatcgg tcgatagttt tcccgcaatc cagcgcaaga taaaatctgc aaagtaattt
      841 gctattgttc gcacgctgat aatacaaatc agcgtaaaca tcgttaccat cattgtttct
      901 tgatcttggt ctagcatgtg atccagtaac gccttggcca ctaaaggcag tgttgcggat
      961 gacacgccaa caatcaaaag aatcgctgcg acaagccaaa agaaaactcg atagggttta
     1021 caccaattaa tcagcaaatg gatcgatttt gtttcactca tggtgcatca tcatcaatgc
     1081 taaaattatt caaaataaat gcaatggctg cgtacataaa gtcatctctt tttagtaaac
     1141 tcacagcata ttcaggattc agtcaattta tcaatccgtt taggaaaact taatttcatt
     1201 cgatcaggac aatgaataca catccatccg aacctaccca acagcgacct tccacaccta
     1261 gaaaaggtat cattctagca ggtggttcag gtactcgact ctaccctgta actcaagtca
     1321 tttcaaaaca actcttacca atttttgata agccaatggt ttattatccg ctgagcaccc
     1381 taatgcttgc agggattcgc gatattctta tcatctcaac accgcaagat accccgctct
     1441 tcaagcaatt attggagaac ggtgaaccct gggggctcaa tttgtcctat gccgtgcaac
     1501 cttcccccga tggcttagct caagccttta tcatcggtga atcgttcttg ggaaacgact
     1561 attctgcatt aatcttaggt gacaacattt tttatggtca cgatttccac catttactcg
     1621 ataacgccac gcaacgcgca acgggtgcaa cggtttttgc gtaccatgtt catgaccctg
     1681 aacgctatgg cgtggttgga ttcgattctg ccggccgagc gatcaatctt gaagaaaaac
     1741 cacagcaacc ccaatcaaac tacgcagtaa cgggcttata tttttatgat cagcaggttg
     1801 ttgaatacgc aaaaagttta aagccttccg cgcgaaatga attggaaatt accgacctca
     1861 atcgaattta tctagaacaa aacaaactcc acgtggaaat tatgggcaga ggctatgctt
     1921 ggttggatac cggcacatac gaatccttac tcgatgcgag caaatttgta gcaaccatag
     1981 aacatcgcca gggattgaaa atcgcatgtc ctgaagaaat cgcatttcgt aaaggatgga
     2041 ttactgcaga gcagttagat aaactggctc gccctttagc taagaatagc tacggtaagt
     2101 atttacagca aattttgaac caacccttat tatgatcgct tgagaaaatt aaggaacaaa
     2161 aaatggaagc cattcaatta gccattccag atgtttattt gattcaaccg cgtgtatttg
     2221 gcgatgaacg aggcttcttt tttgagagct ataatcagca acagtttaat gcagcgatca
     2281 atcgacccat tagcttcgtt caagctaacc attcgcgttc agcgcgccat gtgctgcgtg
     2341 gcttacatta tcaaatcaaa caaccacaag gtaagttggt tcgagtcgtc agcggagaag
     2401 tttttgatgt ggctgtcgat cttcgcaagc actctgctac ctttgggcaa tgggttggtg
     2461 aaattttaag cgctgaaaat aaaaagcagc tctggattcc tgaaggcttt gcacatggct
     2521 ttctcgtgct ttccgaatcg gccgacttcc tttaccaaac aaccgactat tgggcaccgg
     2581 cgtttgaacg gtgcatcctc tggaacgatc ccgtattgaa tattcaatgg ccaatcgatc
     2641 agtctcctgt gctatcgggt aaagatgcac aaggcacatc gtttaagctc gccgaaactt
     2701 acccttgaac actacgttcc cattagcacc aaccgccatt aattactaat atatggcaaa
     2761 gtccaaatcg ctatacttct gcaatgcgtg cggcgggcaa tccttgaaat ggcagggaca
     2821 gtgccctcac tgtcgcgaat ggaatacctt ggttgaaggc gttgctgaaa aaaaagggcc
     2881 aagtttgatt tcaggactaa gaccaactga aatacaaagc ttgagcgata tcgaagtcac
     2941 tgaaacaccc cgtttatcaa caacaatcgg cgaattcgat cgtgtactgg ggggtggttt
     3001 ggtgctgggg ggagtcgtgt tattaggggg agacccgggc atcggcaaat caacactgct
     3061 gcttcaggcg ctttcagcaa tgtcgatgga tcatcgcgta ttatatgtta gcggcgaaga
     3121 atcggcgcag caaatcgctt taagagcacg gcgattaaat ctcgatgtca gtaaagtcag
     3181 cttgttgact gagattcatc ttgaaaatat ccatgcaatg cttagtaacg cagcacctgc
     3241 gataacagtc atcgattcaa ttcaaacaat ctattcagat accttgcaat cagcccccgg
     3301 ttctgtggca caagtcagag agtgtactgc gcaattgacg cgaattgcta aaagtaaagg
     3361 aatttgtatg attctgatcg gtcatgtgac gaaagaaggt tcgcttgccg ggcccagagt
     3421 gcttgagcat atggtcgata ccgttatgta tttcgagggt gatgctcact ctagtttccg
     3481 tttgattcgc tcattcaaga atcgatttgg cgccgtcaat gagctcggtg tttttgcaat
     3541 gacggaaaag gggttgcgcg gtgttaataa tccatccgct ttttttctct ctcaccacca
     3601 taagcctgtg ccaggttcct gcgttatggt cactcaagaa ggaactcgcc ccttattagt
     3661 cgaaattcaa gctttggttg atgacaccca cactgccaat cccaaacgtt tgagtatcgg
     3721 tttagaacaa aaccgactgg caatgctttt agctgttctg catcgccatg ccggaattcc
     3781 ttgtttcaat catgatgtct tcatcaatgc agtgggtggt atccgaatca ccgagcctgg
     3841 ggctgattta gcggtcttag ccgcgattgt ttcatcaatt aaggataaac ctcttgcaga
     3901 aaaaatggtg gtatttggtg agattggctt agctggagaa attcgccccg tacaacgcgg
     3961 ccaagagcgg ctaaaagaag cagcaaagct cgggttcatc aaagccatca tccccgaatc
     4021 aaataagccc aagcaaccca tccctgacat cgaaatcctt accgtaaacc gagtggaaca
     4081 agcattagcg ctactgcgat gaaaagcgtc actacatcca gaaaaattcg tatctatttg
     4141 ttaattttac ttacttgtgc attgcttctg gctgccgctg tgctaccacc ggtctcacaa
     4201 tccaccttat atcatgagtt cgctgatcaa cgctcgtatt ggggtattcc taatttcctc
     4261 aacgtggttt ctaatctaat tttcttgatt gttggcatcg cgggtctaat ttatttgagg
     4321 agggaatcat tattacagca aaatcaaccg ttatccgctg aaataaaatc ataccgactg
     4381 gtatttgcta gtaccgctgc tgcttgcttg ggttcgattt actatcactg ggaacctagc
     4441 ctgattagcc tattctggga tcgattaccc attgcaatcg gatttatgag cttacttgcc
     4501 gcgactgtaa tggaacgctt tgctactaac tgggtaacaa cctattctgt aatattatta
     4561 cctattgctg gcgcaatgag cacactatat tggctctgga gcgagcttta tagcgttggt
     4621 aacttaaatt tctatctggc tgcgcaattt tgttcaatta ttctcattct cctgctggtt
     4681 agcagttttc catctcgcta tagccatgcg tcggagattt atcgcatcct gtccatttat
     4741 ggtttagcta aattagctga gatagctgat caagaagtct atcaattcgg aaacatcatc
     4801 agcggacata cccttaaaca catcctcgct ggacttgcag tttatttcat tatgcgcatg
     4861 ctaaaaatac gaacactgaa accaaaccat gaaatatacg tcagtaaaaa ataaaaaccc
     4921 ctttagcaaa atttgctaaa ggggtggtaa ttttagaagt tactcaagaa ccaaaacgcg
     4981 gtgtacgcga ctgattactt cttcctgaac ctgaaaatga attaaatcca gatgaaaatc
     5041 cactgcgatt tcggacaggt ctttttctag cctcagagcc agctgaaaaa tcattaaacg
     5101 tatgattatc ccgctttgat tgattggagt ggttctgcgt ttttctttct tcagttaatt
     5161 gattacctcc cccccacgac cgatttcgtt tatgcgtgat atttggcatt ctttggtctc
     5221 taccgctgtg tgaacgccca ccaccagtct ttggtttata ttttgactcc attcctggaa
     5281 caacgaaagg ggcaatcgtt tgccctgtaa atcgctctat ttttctcagg ctgatgctgt
     5341 cccttccagt tgcaaatgat actgctacgc ctgatgctcc
//
LOCUS       DAOQLJ010000145         5352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_6948_length_5352_cov_5.512743, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000145 DAOQLJ010000000
VERSION     DAOQLJ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6948_length_5352_cov_5.512743"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..357
                     /locus_tag="PK522_09435"
     CDS             169..357
                     /locus_tag="PK522_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41993.1"
                     /translation="MCHVCDKKNEYLQHDLRKRSFLKLAAASTLGLGISGAGISSVEE
                     ILSGAYLSRRMAAHNLWR"
     gene            complement(368..724)
                     /locus_tag="PK522_09440"
     CDS             complement(368..724)
                     /locus_tag="PK522_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41994.1"
                     /translation="MRISIFSLVVFGLLGFLTSCADLGPYRMDMTQIIESAKTSRDHN
                     WIAGHYEDTAKDMQSKAEEHKKMLAQYEAQRQYYGKRDLDMESMCRALIHTYEQATIE
                     NMNLADAHRKMAEAIK"
     gene            complement(866..3073)
                     /locus_tag="PK522_09445"
     CDS             complement(866..3073)
                     /locus_tag="PK522_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HNC41995.1"
                     /translation="MNSDLNDSHPSDTDDHRQINSKLPDAPLAADEDKWTCPMHPEIL
                     RNAPGDCPICGMALIPITNTNMGESDNSELRDLTRRLWIGIALSLPLIALTMMPMIGL
                     NEPFGLQPRLRGWIEFLLGTPVVLWVGWPILRKFWLSLVHRALNMYTLIGLGVGCAYF
                     FSLAAVLMPDWFPQEFHAHDGAVGTYFEAAAVIVTLVILGDYLQLRAMGQTSQAIQQL
                     LRLAPNQAWRLRDDGTEEQVPLDVIVVGDRLRVKPGEKIPVDGTVLEGMSRVDESMIT
                     GEPMSVAKAPGDKITGATMNSNGSLIIRAEHVGADTLLARIVHMVGEAQRTRAPIQRL
                     ADIIAAYFVQIVIAIAIITALAWWFLGPEPKFTYAFLNAIAVLIIACPCAVGLATPIS
                     MTVAMGQGARMGILFRNAEAIERMRDIDTLVIDKTGTLTLGHPVLTNFIVKGIADDEA
                     LALIASVEQLSEHPIGLAIVENAKARGLTLRPAKSFDAINGLGVQADIDGEYVLVGNY
                     SFLAQHGVDTQPWHHQAEALRTEAKTVVFFAVDGIPVGIAAVADPIKENTPEAIVTLK
                     HLGIRIVMLTGDSQHTANAIARHLGIDEVLAEVLPEDKAGHVKRLQAEGRKVAMAGDG
                     INDAPALAQADVGIAMGTGTDVAMESASITLIKGDLRGIERAILMSHATMRNIHQNLA
                     FAFGYNVLGIPLAAGVLYPAFGFLLSPMFAGAAMALSSVSVVTNALRLNRTRI"
     gene            complement(3140..3493)
                     /locus_tag="PK522_09450"
     CDS             complement(3140..3493)
                     /locus_tag="PK522_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC41996.1"
                     /translation="MKARIFFATLVILASLISCAQMNPHPMEMTSAVRNAKTSADHEA
                     LAKHYEDAVHEMQLKVEEHKKLLEEYEKHPYYYPKRAQDLQGHCWSLIKSYEQAVEAN
                     MSMAKIHHQIADEVK"
     gene            complement(3675..4283)
                     /locus_tag="PK522_09455"
     CDS             complement(3675..4283)
                     /locus_tag="PK522_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNC41997.1"
                     /translation="MFNLLAETLVNALDLREHETGLHSKRVACHTMVLARRFTFDPNV
                     LQQIYWGTLLHDIGKIGISDAILLKAGSLTESEWIEMRTHPEKGYHLISQIPAMTDAA
                     NIILCHEERYDGSGYPKGLSGDRIPLGARLFMVIDALDSITSNRPYRKGHSFEFAKAE
                     ILRMKGSQFDPEAVDAFSAEEEVLQKMVIAKCHNHPSLVSSR"
     gene            complement(4492..>5352)
                     /locus_tag="PK522_09460"
     CDS             complement(4492..>5352)
                     /locus_tag="PK522_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNC41998.1"
                     /translation="AEHKLRIVRAWQAQSAIVAMTGDGVNDAPAIKEASIGIAMGVTG
                     TEVTKEAADIIITDDNFASIVAAVEEGRGIYDNIAKTLAYLLGSSTGELIVMLVAVLL
                     GWPLPLLPLHLLWINLVTDGFAALALSTDPVDSDVLDRPPRHPQTALINRDLIKLTLF
                     TGLLTASVTLGVFAYELYIMGSSLEQARDAAFTTLVIAGLLRAFGARSEQRAIWEISM
                     FSNIRLFLVVAVCFGLQLAIHHVPMFQTLFGVEPITLSQCIGWFAVGFIPLIVLELRK
                     FIRYRLIKEI"
BASE COUNT         1464 a         1285 c         1183 g         1420 t
ORIGIN      
        1 aaactgaaag cttgcatcat tattgattta aaacgagaga aaacgttaaa tggcttattt
       61 ttgagtatat cgaagtatat tataattgca cgaggcggca tgcaaagatc agtaactaat
      121 acctgcttat ttactctgat gtgaatgatt ttaaataccg gagctcttat gtgccacgta
      181 tgtgacaaaa agaacgaata ccttcagcat gatttacgaa aacgttcatt cctgaaattg
      241 gcggcagcct ctacgctggg tttgggtatt agtggtgcag gtatctccag tgtggaagaa
      301 attctttcgg gtgcatacct ttcacgccga atggcggcac acaatttatg gaggtaaagc
      361 aaaagtatta tttaattgct tcggccatct tacgatgggc atcagctaaa ttcatatttt
      421 ctattgtagc ttgctcataa gtatgaatca aagcccgaca cattgattcc atatctaaat
      481 cgcgcttacc ataatactgt ctttgggctt catattgcgc cagcattttc ttatgttctt
      541 ctgcttttga ctgcatatct tttgcagtat cttcataatg tccagcaatc caattatgat
      601 cccgagaagt tttggcagat tcgattattt gagtcatatc catccgataa ggccccagat
      661 ctgcgcagga agtcagaaat cctaacaagc caaaaacaac taacgagaaa attgagattc
      721 tcacagttat atatccttac taattatgat caattaacac cctatcaaaa acagtatata
      781 tcatcatagg ttacagtaca gcataaaatt tagtagcata agcaaatgaa aaataaaaac
      841 caatatccaa ataatttcct agtaatcata ttcttgtacg attaagcctc aacgcgttag
      901 taacaaccga aaccgagctt aatgccatgg ctgcaccggc aaacatgggt gaaagtaaaa
      961 aaccaaaagc cgggtacaat acaccggcag caagcggaat gccaaggacg ttatagccga
     1021 atgcaaaagc aaggttttgg tgaatattac gcatggtggc gtgcgacatc aggatagcgc
     1081 gctcaatacc gcgcagatcc ccctttataa gggtaatact tgcgctttcc attgcaacat
     1141 cggtgccggt acccatcgct ataccgacat cggcctgcgc tagcgcaggc gcatcattga
     1201 taccatcacc cgccatcgca accttgcggc cttcggcttg caaacgtttt acatgcccag
     1261 ccttgtcttc gggaagcact tcggccagca cttcatcaat cccgagatgc cgagctattg
     1321 cattggctgt gtgctgggaa tctccggtca gcatcacaat tctaatgccc aaatgtttga
     1381 gcgttacgat agcttctggc gtgttttcct taatgggatc agcgactgct gcgattccaa
     1441 cgggaatgcc atccacggcg aagaacacca ctgttttggc ttcagtgcgt agagcttctg
     1501 cttgatgatg ccaaggttga gtatccacgc catgttgcgc gagaaagctg taattaccaa
     1561 cgagcacgta ctcgccatca atatctgctt gtacgccaag gccattgatt gcgtcgaaag
     1621 attttgctgg acgcagagtc aaaccacgtg cttttgcatt ctctacaatt gcaagaccaa
     1681 tcgggtgttc cgaaagttgt tctacacttg cgatcagcgc gagcgcctca tcgtccgcaa
     1741 tacccttgac gataaaattg gttagaaccg gatgcccgag tgtcagcgtg ccagttttgt
     1801 ctatcactaa ggtatcaata tcccgcatgc gttcgatcgc ctctgcattg cgaaacagga
     1861 tacccatccg agcgccttgt cccatcgcga cggtcattga aataggtgtt gcaagaccga
     1921 ccgcacaagg gcaggcgatg atgagaacgg caatggcgtt aagaaatgca taagtgaatt
     1981 ttggttcggg gcctagaaac caccatgcta gtgcagtgat aattgcgatg gcaataacga
     2041 tctgaacaaa gtaagcggcg atgatatccg caagccgctg gataggcgca cgcgtgcgtt
     2101 gggcttctcc gaccatgtga acgatacgtg ccagcaatgt gtctgcgccg acgtgttcag
     2161 cgcggataat caatgagcca ttactattca tggttgcacc ggtgatcttg tcgcccggtg
     2221 ctttagctac cgacataggc tcaccggtaa tcattgattc gtcaactctg ctcatgcctt
     2281 caagtactgt gccatcgaca ggaatttttt ctccaggttt tacgcgtagc ctgtcaccta
     2341 caacaattac atcaagcggt acttgttcct ccgttccgtc atcgcgcaag cgccaagctt
     2401 ggttgggtgc aagcctcaac aattgttgaa ttgcctgact tgtctgaccc atcgcacgca
     2461 actgaaggta atcgccaagt atcacaagtg ttacgataac agctgctgcc tcaaaataag
     2521 tgcccaccgc accgtcgtgc gcgtgaaact cctgtggaaa ccagtcaggc ataagtactg
     2581 cggccagact gaagaaatag gcacatccga cgccgagtcc gattagtgta tacatattga
     2641 gcgcgcggtg tacgagtgag agccagaact tgcgcaggat aggccaaccc acccacagca
     2701 ctactggcgt acccagcagg aattcgatcc aaccacgtaa acgaggttga aggccaaatg
     2761 gttcatttag accaatcatg ggcatcatcg tgagagcaat cagtggaagc gacaaagcaa
     2821 tgccaatcca caaccgtcgc gtgagatcgc gaagctcgga attatccgat tcgcccatat
     2881 tcgtatttgt aataggtatc agagccatac cgcagatcgg gcagtctccc ggtgcattcc
     2941 gcaagatttc cgggtgcatt ggacatgtcc atttatcttc atccgctgct agcggcgcat
     3001 ctggaagctt ggagtttatt tgccgatgat catcagtatc tgacggatgt gaatcgttta
     3061 aatctgaatt cattttgacc ctcccataaa tcatatctaa cctgaaggct acgagtctgt
     3121 cgttcttaag ttgtggacat tacttcactt catccgctat ttggtggtga attttcgcca
     3181 tgctcatatt tgcttctaca gcttgctcat aactttttat cagactccaa caatgccctt
     3241 ggaggtcttg ggcgcgcttg ggatagtaat aagggtgttt ctcatattct tcaagaagtt
     3301 ttttgtgctc ttctactttt agctgcattt catgaactgc atcctcatag tgtttggcca
     3361 atgcttcatg gtcagcacta gttttggcat tacgaaccgc gctggtcatc tccatcggat
     3421 gggggttcat ttgtgcacag gaaatcaatg aagccagaat caccaaagtt gcaaaaaata
     3481 tacgtgcttt cataagtatc tcctggtagt tgaatcaaag aaatatacct aaattctata
     3541 atttaattcg taatcctcgg tataaagtag ccaccgagta tccccaatgc tgtgataatt
     3601 gaaagaatac gccgtcctta gcggttagtg agtgaactcg ctgttggtga caagatcaag
     3661 ccctacaata tagttcatct gcttgacact aacgaaggat gattatggca tttagcaata
     3721 accatttttt gcagcacttc ctcttcggcg ctgaaagcat ctacggcttc cgggtcaaac
     3781 tggcttccct tcatacgtag aatctctgct ttggcgaact caaaagaatg acccttacga
     3841 taaggccgat tagaggttat cgaatccaag gcatctatta ccatgaatag acgtgcacct
     3901 aatggaatac gatcgccact tagtcctttt gggtaacctg aaccatcata gcgttcctca
     3961 tgacagagaa tgatatttgc tgcatctgtc atggcaggta tttgcgaaat gagatggtag
     4021 cctttttctg gatgtgtccg catttcgatc cattcagatt ctgttaaaga gccagctttc
     4081 agtaaaatgg catcggagat accaatcttg ccaatatcat gcagtaatgt tccccagtat
     4141 atttgctgca atacattcgg atcgaaggta aagcgacgtg cgagcaccat cgtatggcag
     4201 gcgacgcgct tggagtgcaa acccgtttcg tgctcccgca gatccagcgc attcaccaaa
     4261 gtttctgcta agaggttgaa catatcttcc tgactggcac agacatctat ggttttgagg
     4321 aaacaatgtt cggaacagta tgtagcgccc acagaaatat atggttgcct ttcaagaagc
     4381 tttccacaat gttggcaata cgcttctggg tgcctgcctg ttttgttcat ttgatccccg
     4441 tctaacatgt gtgctccatt agtacacaga gatcaatata attattattt ttcaaatctc
     4501 ctttataaga cgatagcgaa tgaattttct cagctccaga acgatcaagg gtatgaatcc
     4561 taccgcgaac catcctatgc attgacttag cgtaataggt tcaaccccaa ataaggtttg
     4621 aaacatcggg acatggtgta tcgccaactg caagccaaaa catactgcta caaccaaaaa
     4681 taaccttata ttggaaaaca tgcttatttc ccaaatggca cgctgctcgc ttcgggcccc
     4741 aaaagcgcgt agcagcccag caatcaccaa agtagtaaat gctgcatcgc gagcttgttc
     4801 aagactgcta cccattatat agagttcata ggcaaataca cccagcgtga cgctggcggt
     4861 cagtaagccg gtaaatagcg ttagcttgat taaatcacga ttgattagtg ccgtttgtgg
     4921 atgccgcggt ggacgatcca atacatcgga gtcaactgga tcggtcgaca acgccagggc
     4981 tgcaaaacca tcagttacca gattgatcca cagaagatgc aagggcaata gagggagcgg
     5041 ccagccaagt agaactgcga ccagcatcac gattaattcg ccggtactgc taccaagcag
     5101 ataagccaaa gttttagcaa tgttgtcata aatgccccgt ccttcttcca cagcagcgac
     5161 aatagaagca aagttgtcat cggtgataat gatatcggcc gcttccttgg tcacctcggt
     5221 gccagtaacg cccatagcga taccaatgga tgcttccttg attgcgggag catcattcac
     5281 tccgtctccg gtcatcgcca ctatcgcact ctgggcctgc caagcgcgaa caatgcgaag
     5341 tttgtgctcg gc
//
LOCUS       DAOQLJ010000146         5214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_7250_length_5214_cov_6.930219, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000146 DAOQLJ010000000
VERSION     DAOQLJ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7250_length_5214_cov_6.930219"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PK522_09465"
     CDS             <1..256
                     /locus_tag="PK522_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-(glutamine-N5) methyltransferase,
                     release factor-specific"
                     /protein_id="HNC41999.1"
                     /translation="LDQGDLRFEPQIALSAPEDGIACIRTIIAQAPQFLCAGGQLLLE
                     HGYNQAAQCRQLLAQEASFINIFSYPDLAGILRITGGQRI"
     gene            403..711
                     /gene="grxD"
                     /locus_tag="PK522_09470"
     CDS             403..711
                     /gene="grxD"
                     /locus_tag="PK522_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HNC42000.1"
                     /translation="MTTSESIEQLISSNPVVLYMKGTPSQPQCGFSANAVKILNACGV
                     TNFVAVDVLSDPEIRQGIKDFSNWPTIPQLYVNGEFVGGSDIMTEMYQSGELKKLLEK
                     "
     gene            complement(740..1159)
                     /locus_tag="PK522_09475"
     CDS             complement(740..1159)
                     /locus_tag="PK522_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HNC42001.1"
                     /translation="MDEESSLIALFTSSFLAATLLPGGSEAVLIGVLMAYPELFTSAL
                     SVATIGNTLGGMSSYLIGRFLPDEKAILKKTNNSLRSLEWIHRHGSPILLLSWLPIIG
                     DALCVAAGWLRIHWFYAILFIAVGKLARYWVIATAIS"
     gene            1240..1851
                     /locus_tag="PK522_09480"
     CDS             1240..1851
                     /locus_tag="PK522_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42002.1"
                     /translation="MGILSIIIFALCVGLLAGAGAGNDHILPITLITAVVLIASRDKI
                     AARSNAVSDELSNSDLQSNHHLENYYTWPELNQFQYEVKSNSYQGAINQLLKENISPP
                     AAVSIDVPLILNAHLIPDSDHLYDKNAVRIDIHDRTIGYLSPEEALIFYQRLSQQGLT
                     NQVTMCRVLIDKKRDKVNKTNIYKVKLDIEPFLVPEKSNIKSE"
     gene            2004..2216
                     /gene="rpsU"
                     /locus_tag="PK522_09485"
     CDS             2004..2216
                     /gene="rpsU"
                     /locus_tag="PK522_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNC42003.1"
                     /translation="MTTVKVKENEPFEVAMRRFKRAVEKTGLLTELRAREFYEKPTAE
                     RKRKLAAAVKRTYKRLRSQLLPPKLY"
     gene            2247..2699
                     /locus_tag="PK522_09490"
     CDS             2247..2699
                     /locus_tag="PK522_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNC42004.1"
                     /translation="MDLKQKIADDMKAAMRSGDSKRRDAIRLLQAAIKQREVDERITL
                     DNVAITAVIEKMLKQRKDSITQYEMAKRQDLAEAEKFEIEVLQTYMPQPLSEEEIIQI
                     INEIIASVGQLDQQSMGKVMAALKPKLAGRADMGYVSGLIKEKIKALT"
     gene            2788..4620
                     /gene="dnaG"
                     /locus_tag="PK522_09495"
     CDS             2788..4620
                     /gene="dnaG"
                     /locus_tag="PK522_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNC42005.1"
                     /translation="MISQSFIQELLNRIDIVDIIEQSIPLKKSGSNYVACCPFHNEKT
                     PSFSVNQSKQFYHCFGCGKHGNAINFVIEFSGISFVDAVENLAVQAGMPMPDKTVITA
                     AANRSSSRLKVDTEIDNQEPIEKKQDNQVMQRMTDQLQRAADYYREQLKHATKAISYL
                     KQRGLSGEIAARFKIGYAPDELRSLSQIFTDYDRDESENLLLKNGLIIKSNNGRYYDR
                     FRARIIFPILNQKGQIIGFGGRVIDEKGGPKYLNSPETSLFIKNRELYNLFLARKAIR
                     ESRRVLVVEGYMDVVVLSQYGIEYVVATLGTATTSSHIQKLLRQTDEIIFSFDGDNAG
                     KKAAWRALETSLAHLKDGKKISFLFLPDGEDPDSFIRKFGKQSFESLFEKAIPLSDLL
                     IGELSSRVNLESSEGRAALIQIAKPLIQLVSAPILKLILIKRLSQLTGVNQEALTKIF
                     ESKLKNANSQSSKQIESLRLQPISPYHKLIKVLLDDPRFVNKLDRDLSCLLNAAPQQT
                     VLALEALVSFYKSLSSQELETQKITETLLYFSETMHAEELNKTTELKLGWETEIDLEA
                     EFLGLLSRIKDMQRKERMTVLQNKPLNLLTEQEKRELQQLANSS"
     gene            4845..>5214
                     /locus_tag="PK522_09500"
     CDS             4845..>5214
                     /locus_tag="PK522_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HNC42006.1"
                     /translation="MPKDNNMSNKPTKREKATKNSPTTEQLEITEEQSLEENEVMKGA
                     KSSKNNKVAKKLKAAVASKEMVDTPVTPEVVAPTAQDIEAKKTRLKKLIVQGKERGYL
                     TYAEINDHLPDDMLDAEQIEG"
BASE COUNT         1696 a          867 c         1099 g         1552 t
ORIGIN      
        1 ccttgatcaa ggggatttac gcttcgaacc tcagattgcg ttgtcagcac ctgaagatgg
       61 tattgcatgt attcgcacca ttattgcgca agcccctcaa tttctgtgcg ctggtggaca
      121 gttactattg gaacatggtt acaaccaggc tgctcaatgt agacagctcc ttgcacaaga
      181 agcaagtttt attaatatct tttcataccc tgatttagca ggtattctac gcataacagg
      241 tgggcagcga atctgattgt attggattac gtgtgaatca gtaaagatgg ttatccattt
      301 gcaagtgatc aatatttgct tagacccctg atgactaact ttttatatga gcagtagctg
      361 atttttatta attaatttta ttaatttgtg aggattttga gtgtgactac cagtgaatca
      421 attgagcaac taatcagttc aaacccagtt gttttatata tgaaaggtac tccaagccaa
      481 ccacagtgtg gtttttctgc taatgcagta aagatattga atgcctgtgg tgtaacaaac
      541 tttgttgcag tagatgtttt gagtgaccct gaaattcggc aaggaattaa agatttctct
      601 aactggccaa cgattcctca actatatgtc aatggtgaat ttgtcggagg ttcggatatc
      661 atgacggaaa tgtaccaaag tggtgaactc aagaaattac tagagaagta actttttcct
      721 gagcgtactt agttgtgcgc tagctgatcg cggtggcgat gacccaatag cgggctaatt
      781 ttccaactgc aatgaataag atagcataaa accagtgaat gcgaagccaa cctgctgcaa
      841 cacaaagtgc atctccaata atgggtaacc acgacagcag taatatgggg gagccatgac
      901 gatgaatcca ctcgaggctt cgcaggctgt tattagtttt cttcaaaata gctttttcat
      961 ccggaagaaa ccgaccgatc aggtaagagc tcattccacc tagggtattg ccgattgttg
     1021 caacacttaa tgctgaagta aataactcgg gataagccat tagcacaccg atcaatacag
     1081 cttctgaccc cccgggtaga agggttgcag ctaagaaact acttgtgaat agcgcaatta
     1141 agcttgattc ttcatccatg acgttatgta attaaatgag gttaattctg aattaattta
     1201 taccattttt ggcaggtaca aaaattgaag tctgagaagt tgggtatttt atctattatt
     1261 atttttgcat tgtgtgttgg ccttcttgca ggggcaggtg ctggtaatga tcatattctt
     1321 cctattacac tgattacagc tgttgtttta attgcgtcac gcgacaaaat tgctgcaagg
     1381 agtaatgctg tgagtgatga gctgagcaat agcgatttac aatcaaacca tcatttagaa
     1441 aattattata catggccaga attgaatcag tttcaatatg aggttaaaag taattcttat
     1501 caaggtgcga ttaatcaact actaaaggag aatatatcgc cccctgccgc agtatctatt
     1561 gacgtaccac tgattttaaa tgcacattta attcctgata gcgatcattt atatgataag
     1621 aacgctgtta gaatcgatat ccatgatcga actatcggat atttaagccc agaagaagca
     1681 ctgatttttt atcagcggct aagtcagcag ggtttgacca atcaagtaac gatgtgcagg
     1741 gtacttattg ataaaaagcg tgataaagtg aataagacca atatttataa agtgaaattg
     1801 gatattgagc cttttcttgt tcctgagaag agtaatataa agagcgagta ataatgttgt
     1861 tgggtgatcc tgtcctttaa ttgattagga ttggggaaat aaaaaattaa ttaatagaaa
     1921 aaacttgggt aattctactt aaaacactgt tataattgcg ggtttttaga attctctagt
     1981 gagaaagggg taataaagat tttatgacta cagttaaagt taaggaaaat gagccatttg
     2041 aagttgcaat gcgtcgtttt aaacgcgcag tagaaaaaac aggtttactg accgagttac
     2101 gagctcgtga gttttacgaa aaacctactg ctgagcgtaa acgtaaactt gcagccgcgg
     2161 tgaagcggac ttataaacgg ttacgtagtc agctgttacc tcctaagctt tactagtaga
     2221 gtctcgattt attgaccatc gtgatcatgg acttgaaaca aaaaattgca gatgatatga
     2281 aagcagcaat gcgttcaggt gattctaagc gacgtgatgc aattcgattg ttacaagccg
     2341 ccatcaagca gcgagaagtc gatgaaagaa ttacactgga taatgtagcg attacagcag
     2401 tcatcgaaaa aatgctgaag cagcgaaaag attctattac acagtatgaa atggctaaaa
     2461 gacaagattt ggccgaagca gaaaaattcg aaatagaagt tttgcaaacc tatatgccac
     2521 aacctttgag tgaagaggag attattcaaa tcataaatga aataattgcc tcggtcggtc
     2581 aattagatca gcaaagcatg ggtaaggtta tggcggcatt aaagccaaaa ttagcggggc
     2641 gcgcagatat gggttatgta tctggattaa ttaaagagaa aataaaagca ctgacttaat
     2701 cgagtattcg taggtagatt cttgaggatt taattaagga aagtcattgg gttgttttaa
     2761 gtcttaacta ccttccctat ttaataaatg atttcacaat cgtttattca ggaattactt
     2821 aatcgcattg atatcgttga tattatcgag caaagtattc cattaaaaaa atcgggtagc
     2881 aattatgttg catgctgccc tttccataat gaaaaaactc cctcattctc agtaaatcaa
     2941 tcgaaacagt tttatcattg ttttgggtgt ggtaagcacg gaaacgctat taattttgta
     3001 attgaattta gcggaattag ctttgttgat gcagttgaaa atttagctgt tcaggctggt
     3061 atgccaatgc ctgataaaac ggttattact gcagcagcta atcgatcaag ttcaagatta
     3121 aaagttgata ctgagatcga taaccaggag cctattgaga aaaaacagga taatcaagtt
     3181 atgcaacgca tgactgatca gctgcagcga gcagctgatt attatcggga acaattgaaa
     3241 catgcaacta aggccatatc atatttaaaa caacgtggat tatcgggaga aattgctgct
     3301 cgcttcaaga ttggttatgc gcccgatgag ctaaggagtc tctcacaaat ttttactgac
     3361 tatgatcgag atgaatcaga aaatttatta ttaaaaaatg gcctgattat taaaagtaat
     3421 aatggtagat attatgatcg gttccgagcg cgtattattt ttccaattct taatcaaaaa
     3481 gggcagatca ttggctttgg tggacgagtg attgatgaga agggtgggcc aaaatatttg
     3541 aattcgcctg aaacatcgtt gttcattaaa aatcgggaat tgtataattt atttttagct
     3601 cgcaaagcga ttcgagaatc aaggcgtgtc ctggttgttg aagggtatat ggatgtcgtt
     3661 gtgctgtcgc aatatggcat cgaatatgtt gtagcaacct tagggacagc gaccacctcg
     3721 tcacatattc aaaagctttt gcgccaaact gatgaaatta tatttagctt tgatggtgat
     3781 aatgcgggta agaaggcggc ttggcgggca ttggaaacga gtctcgctca cttgaaggac
     3841 ggtaaaaaaa taagttttct ttttttaccc gatggcgaag atcctgatag ttttattcgt
     3901 aaatttggta agcaatcatt tgaatcgctc tttgagaaag caattcctct atctgattta
     3961 ttgatcggag aattgtcttc gcgagttaat cttgaatcga gcgagggtcg ggccgcattg
     4021 attcaaatcg caaagccatt gatccaatta gtttctgcac caatattgaa attaatatta
     4081 ataaaacggc tttctcagtt aactggagtg aatcaagaag cgttaaccaa gatttttgaa
     4141 agtaagctta aaaatgctaa ttctcagtca agtaagcaaa ttgaaagttt gagattgcaa
     4201 cctatctcac cttatcataa attaatcaaa gttttgctcg atgatccacg ttttgtaaac
     4261 aaattagatc gtgatttatc ttgtcttctt aacgctgcac ctcagcaaac tgtgcttgca
     4321 ttggaagcgt tagtgagttt ttataaatcg ttatcgtcac aggaattaga aactcagaaa
     4381 attactgaaa cgctattgta tttttctgaa acgatgcatg ctgaagaact caataagaca
     4441 acagaactca aattaggatg ggaaactgaa atcgatttgg aagcagaatt tttaggctta
     4501 ttatctagaa ttaaggatat gcaacgtaag gaaagaatga cggtgttgca aaacaaacca
     4561 cttaatttgc taactgagca ggaaaaaaga gaattacagc agctggctaa ttcatcctaa
     4621 ctagagcaac aatttaattt ttattgatat ttatttttcc acatgataga gaataggtag
     4681 gattaattat tatttctata tataatatct ggctttatca agtattgcct aaagtcgttg
     4741 ccttaattgc tgataaagtt ttgtagccta aacccagata ctagatgatt caattgatag
     4801 gctcaggcca tttctaataa caattttgag tgattatcac gaagatgccc aaagataata
     4861 acatgagtaa taaaccaacg aagcgagaaa aagctactaa aaattcgccc actactgagc
     4921 agctagaaat tactgaagag cagtcgttag aggaaaatga ggttatgaag ggcgcaaagt
     4981 catcaaaaaa taataaagta gcaaagaaac taaaagctgc ggttgcctcc aaggagatgg
     5041 ttgatacacc tgttactcct gaagttgttg cgcctacggc tcaggatatt gaagccaaaa
     5101 aaacacgcct taaaaaattg attgttcaag gcaaagaacg tggttattta acttatgccg
     5161 agattaatga tcaccttcct gatgatatgc ttgacgcaga gcaaattgaa ggtg
//
LOCUS       DAOQLJ010000147         5206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_7265_length_5206_cov_6.026014, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000147 DAOQLJ010000000
VERSION     DAOQLJ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7265_length_5206_cov_6.026014"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PK522_09505"
     CDS             complement(<1..249)
                     /locus_tag="PK522_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42007.1"
                     /translation="MNNEHSQPKTSQYPILGVRQVKKTSLLVAGYQKIVALLNNSADQ
                     LPPFLLRLVLAYEFWEAGIMKIESLRVFRRPFSLSQATL"
     gene            complement(334..1077)
                     /locus_tag="PK522_09510"
     CDS             complement(334..1077)
                     /locus_tag="PK522_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42008.1"
                     /translation="MDNGKLEMDENFFSQLSREQLERMAEAGAQIIECYRVLQKAGLN
                     VVGEVLKGQGEFYELEHYPKDDVYDGETHSQYYYHAHRSETNENGHFHTFLRQPGMPA
                     GIFPIPYSGTESWPEGKDALSHLIGISMDAYGFPLGLFATNRWVTAEAWYKSEDVISM
                     LDHFKIDHAYPSWPVNQWISAMLILFRPQIEALLRQRDQVITERARKHQGIDVFEERS
                     LELTGYLPINVEQQIKYTVAVIENHNFKA"
     gene            complement(1129..1806)
                     /locus_tag="PK522_09515"
     CDS             complement(1129..1806)
                     /locus_tag="PK522_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNC42009.1"
                     /translation="MKSKIQPLRATVISALFGAVIGLPLTLNGCAMATCRPCVPRAGK
                     RNPCNPCAAKNPCAAMNPCAAKNPCAAGDRVDPKAVTRPEGTKPYQGDRAALLNEGKA
                     LWNSKEISRNNSMSCSTCHQDGTAMLQPDFAKPYPHFVQMAADSAGKASITLEEMVQL
                     CMIKPMQSKPFAWDSRQLAALTAYTSVLQKAFKPVGNAANPCAAKNPCAAKNPCAIRN
                     PCAVKPN"
     gene            complement(1962..2177)
                     /locus_tag="PK522_09520"
     CDS             complement(1962..2177)
                     /locus_tag="PK522_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42010.1"
                     /translation="MKARQKLLASSIVASLMAISMSAPTFAESNPCAPKAKAQHQHDM
                     KNPCAGKNPCAAKNSCAAKNPCAAKKQ"
     gene            2628..3113
                     /locus_tag="PK522_09525"
     CDS             2628..3113
                     /locus_tag="PK522_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HNC42011.1"
                     /translation="MAFNAYLNCESIKGESTDQNHPDWIEVVSFSHSLNQPTSGTNGT
                     GGRGAGRVDFASFVITKSVDKASVDFNIFCAKGTHIPKVILEICQETGQNICYLRYEL
                     ENVMIQSVSTSGDSSGRPHETIAFVYDKISWAYTLINNDGSAGTTVGPKKWNIEINTV
                     E"
     gene            complement(3176..3652)
                     /locus_tag="PK522_09530"
                     /pseudo
     CDS             complement(3176..3652)
                     /locus_tag="PK522_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008186715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(3895..5037)
                     /locus_tag="PK522_09535"
     CDS             complement(3895..5037)
                     /locus_tag="PK522_09535"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HNC42012.1"
                     /translation="MRFEEAYEGYRRGSLTQAEGALLLGVCDRTFRRYMNRYDEGGLD
                     ALLDKRLVQISHRRALVDEVMRTVDLYRDRHLGWNAKHFYAWHRKEIGTRSYTWVKSR
                     LQEAGLIERAKKRERAPLVGMMIHQDGSTHEWITGEKWDLIVTMDDATSEHYSMFFVE
                     EEGTASSFQGVQEVIEEQGLFASFYSDRGSHYWHTPEAGGKVDKQNLTQFGQALKRLG
                     IEMIAAYSPEARGRSERMFRTHQERLPKELALAGITHMAAANRYLKEIYMPAFNAEFM
                     QSASEEGSAFVPWIGGQLADILCERHERVVGHDNCVSFEGLKLQIPADRHRCHYVKAR
                     VTVLRYPNRQMAILHGPRRLATYDALGKEIKLSNTVSRKSAATTAQ"
BASE COUNT         1368 a         1185 c         1100 g         1553 t
ORIGIN      
        1 taatgtggct tgactcaaac taaaaggtct ccggaaaacc cggagcgatt caattttcat
       61 aatacccgcc tcccaaaatt cataagctag gactagccgt agcagaaatg gcggtagctg
      121 atcagcgctg ttgttcagta aagctacaat tttttgatag ccagcaacta acagtgaagt
      181 ttttttcacc tgacgaacgc ctaaaatggg gtattgtgag gttttgggtt gagaatgttc
      241 attgttcatg taaagctcca gtagtaatct gctgtattcc tagttgtcgc aaggaagaaa
      301 catactgata cgattgatat aaaaaaacaa atatcacgct ttgaagttgt gattttcaat
      361 cactgccaca gtgtatttaa tctgctgctc aacattgatc ggtaagtaac cagtcagctc
      421 aaggcttctt tcctcaaata catctatgcc ttgatgctta cgtgcccgtt cagtaataac
      481 ctggtcacgt tgtcgcaata gtgcttctat ctgtgggcgg aataaaatca gcatggcgga
      541 aatccattga ttaaccggcc acgaaggata tgcatgatct atcttaaaat gatcaagcat
      601 gctaatgacg tcttcggact tataccacgc ttccgccgtt acccatcgat tggtggcaaa
      661 caatccgaga ggaaaaccgt aagcatccat tgaaatgccg ataaggtggg aaagtgcatc
      721 tttaccttcc ggccaggact ctgtaccgct gtaaggaatg ggaaaaatcc cggcgggcat
      781 tccaggttga cgcaagaaag tatgaaaatg tccgttttca ttggtttccg agcggtgcgc
      841 atgatagtaa tactgactgt gtgtttcgcc atcgtacaca tcgtcttttg gatagtgttc
      901 taattcgtaa aactcacctt gcccttttag aacttctcca acaacgttaa gtccagcttt
      961 ctgtagcacc cgatagcatt cgataatctg tgcgccagct tcggccattc gctcgagctg
     1021 ttcacgggat aattgtgaaa aaaaattttc gtccatttcc agtttaccgt tatccataaa
     1081 aacgatgaag cctaagtcag cgcttcatcc ctccccttga ctcagtaatt aatttggttt
     1141 aacagcacat ggatttctta tagcacaagg gttttttgct gcacatggat tcttggctgc
     1201 acaaggatta gctgcattac caaccggttt aaatgctttt tgcaataccg atgtataagc
     1261 cgtcaacgct gcaagttgcc gggaatccca tgcgaaaggc ttactttgca tgggtttgat
     1321 catacaaagt tgaaccattt cttctagtgt gattgatgct ttaccagcgc tatcagcggc
     1381 catttgcaca aagtgtggat aaggtttagc aaaatctggt tgcaacattg cagtgccatc
     1441 ttgatgacag gttgaacacg acatcgaatt gttgcgacta atttctttac tattccataa
     1501 cgctttgcct tcatttaata aagccgcccg gtctccttga taaggttttg ttccctcggg
     1561 tcgagttacc gccttaggat ccactctatc acctgcagca caaggatttt tagcggcaca
     1621 tggattcatg gctgcgcagg gattttttgc cgcacaagga ttacacggat tacgtttacc
     1681 agcacgcggc acacatggcc tgcaagttgc catggcacag ccattcaatg tcaaaggcaa
     1741 cccgattact gctccaaata aagcagaaat gactgttgct cttaatggtt gaattttaga
     1801 tttcataatt ttccttacgt tacagaaaag ctcttatgtt gcgacaagtg cattaattaa
     1861 agcaccccgc gctagaagca cggggtatta gctgcgctct tcagtctgag ggtttttaac
     1921 cagcttttcc taagaagaac aggggattga actcgctgag attattgttt ttttgctgcg
     1981 caaggattct tcgcagcgca ggaattcttg gccgcacaag gattcttacc agcacaggga
     2041 tttttcatgt catgctgatg ttgtgctttc gcctttggtg cgcacggatt actctcggca
     2101 aatgttggtg cgctcataga aattgccatc aagctagcta ctatggaaga agccaaaagt
     2161 ttttgacgag ctttcattat cagttctcct aagattaaat tatggtatgc attaaacaac
     2221 caccatttgg tggtttaata cctgaagtcg gaactagttc ccctaattcc tgatgcttag
     2281 tcgaatgaaa taggattccc tgacaatatt tttacctctt attcagttta acaaattaac
     2341 tttatgtgcg atctcggttc attgctttta ccgtacagtt attaacagaa ctccaaggga
     2401 gattctccgc ttttgcttct gtgcgggtcg ccactcctga ttacagacct atcgccttga
     2461 atctctgcct catttgtgtt ttcgtgttta cttctcagtt agttggtggt caggaatttt
     2521 cgatagacaa tgtttgtgta agagataatt gaagatacaa gatagtttac gattaatcgc
     2581 ttatactcca ttctgttgca taaactacta aattagggag tgtaaaagtg gcttttaatg
     2641 catatctaaa ttgcgaatcg atcaaaggcg aatcaacaga ccaaaatcat ccagattgga
     2701 tagaagttgt tagctttagc catagtctaa atcaacctac ttcaggcact aatggaacag
     2761 gaggacgtgg agcggggcgt gttgattttg catcattcgt cattaccaaa agtgttgata
     2821 aagcgagtgt tgatttcaat atattctgtg caaaaggaac acatattccc aaagtaatcc
     2881 tcgaaatctg ccaggaaact ggtcaaaaca tttgttacct tagatacgaa cttgagaatg
     2941 tgatgattca atctgtctca actagtggag atagttcggg ccgtccgcac gaaacaatag
     3001 ctttcgttta cgacaaaata tcatgggctt atacactgat aaataacgat ggttctgctg
     3061 ggacaacggt aggtcccaaa aaatggaata ttgaaataaa tactgtcgag taattaagat
     3121 gatggctaat attgtattaa aatgtgatgg aaacgacttc ggtagactga aataattatc
     3181 tcattttttc gattaaacgt tcatacggag tttttcctcc caaacttcca tgaggtcggt
     3241 gatagttgta gaaagcttcc cactcggcta atttggcatg caggtcaaca tcatctgtat
     3301 aagttaataa ctggtaaaat tcccggtgat cagtgccatg cgaacgttcc accttgccgt
     3361 ttaatcgagg cgttctgggt ttgatgtaag aatgtcgcac acccatgtct tccaggtgcc
     3421 aatggaattt agcttggaat tcatggccat tatcagttcg gatagtattg attctgaaag
     3481 ggaattttga gataacataa tctacgaaat caatcgcgtt ctgctgtgta tgtcgctcat
     3541 aaattttcag cgcgcggatc cgcgttgcgt catcaatggc ggtatattgg aagcgccgga
     3601 ttttcttacc atcttcaact ttaaaatcga gaaacttcac atcaacttgt actagcttag
     3661 acctgatctg acagttacca gtttttttag gtgtctgtca gtttagattt gagctaaatt
     3721 tttttctgcc caaattgatt tgccatcaag taatgtatcc aatggtgttc gtccacagca
     3781 cttttaattt ctacaatcgg aagacggaag ccccgatgtt agcaacaaat agaactgtcc
     3841 ggttttgtag caaataaatt gtccgctttg aaccgcattg aattatcact ttattcattg
     3901 cgcagttgtc gcggcggatt tacgcgaaac tgtgttgctt agttttattt ccttaccaag
     3961 cgcatcgtag gtggcaagcc tccttggtcc gtgcaagatt gccatttgtc tgtttggata
     4021 gcgtagcaca gtcactctgg ctttcacata atgacagcgg tgacgatctg ctgggatttg
     4081 tagtttcagg ccttcaaaac taacgcagtt atcgtggcct acgacgcgtt catgccgttc
     4141 acagaggatg tcggcaagct gtccaccgat ccagggtaca aaagcactgc cttcctcgct
     4201 tgccgactgc ataaactcgg cattgaaggc aggcatatag atttctttca agtatcgatt
     4261 ggctgcagcc atatgggtaa taccggctag cgccagttcc ttgggtaatc gctcctggtg
     4321 tgtgcgaaac atgcgctcac tgcgtccacg cgcttcgggt gaataggctg cgatcatttc
     4381 gattcccagt cgcttcaggg cctggccaaa ctgggtaagg ttctgtttgt ctactttgcc
     4441 gcccgcttcc ggtgtgtgcc agtaatgact gccgcgatcc gagtaaaacg atgcaaacaa
     4501 cccctgttcc tcaatgactt cttgtacacc ttgaaagctg ctggctgtgc cttcttcctc
     4561 aacgaagaac atcgagtaat gctcactggt ggcgtcatcc atcgtgacga tcaaatccca
     4621 tttctcccca gtaatccatt catgcgtact accatcttga tgaatcatca ttcctaccag
     4681 cggcgcacgt tcacgcttct tggcgcgttc aatcaacccg gcttcttgca accggctttt
     4741 aacccatgtg tagctgcgcg taccgatctc tttccggtgc cacgcataaa agtgcttagc
     4801 gttccaaccc aggtggcggt cacgatatag atctactgtt cgcattactt catccaccag
     4861 tgcccgacgg tgcgatatct gaacgagccg tttatccagc agcgcatcca gaccaccctc
     4921 gtcatatcgg ttcatatagc gccggaatgt gcgatcacat actcccaata acaatgcccc
     4981 ttccgcctgc gtcaaactcc cccttctata gccttcatac gcttcttcaa atctcatctt
     5041 cctggtctcc tgtagccact ccgtccgttt catttgaaat cctcctcgga tttcttataa
     5101 aaccggacag atcatgtgct acaaaccagg acagtttatt tgctcgctac aaaatctgaa
     5161 taaagacttg cctgaataat gaattggctt gtattatcta ctcata
//
LOCUS       DAOQLJ010000148         5100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_7528_length_5100_cov_5.148464, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000148 DAOQLJ010000000
VERSION     DAOQLJ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7528_length_5100_cov_5.148464"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PK522_09540"
     CDS             complement(<1..570)
                     /locus_tag="PK522_09540"
                     /inference="COORDINATES: protein motif:HMM:NF022673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HNC42013.1"
                     /translation="MKREHIDQWKHHFLDCSPMFEPLRQAGQLLNQLEQWPTPDQLNH
                     LLSVQKSNIRTNTHHPLRFVTQTTPTSLFEQQYEPRTYLCGELQTRAQNWHDLFNALV
                     WMTFPQSKAQLNALHYHTLKIAEKQPRSARGRLRDAITLLDESGILVTSSHEDLTCLL
                     KNFEWKTLFWENRSDLLQHMKFFLFGHSLY"
     gene            complement(567..1832)
                     /locus_tag="PK522_09545"
     CDS             complement(567..1832)
                     /locus_tag="PK522_09545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC42014.1"
                     /translation="MHDTEQILQTGESSELVELGTVPHFTPIQERPIIAVLAGIHFIH
                     IMDFMMLLPLGPQLMKLFELTPQEFSLLISIYAFSAGAFSFFASFIIDHYNRKSFLIF
                     LCGGIGLATLLCALSFEYYLLLLARMVAGGFSGVLSATIFTIVADVTPAHRRGNATGT
                     VMSAFSAVAVIGMPLGILLVNLFSWRAPFLFLTLISLVMIVLAIWLLPSLHGHLKHRQ
                     HRHPAQHLRAVFYNRNHLYAFALIAMLMFAGFSVSPFITTYMITNIGMSEVDIPYLYF
                     CGGLATFYTARLFGKLSDQHGKHKIFSIAAALSVFPILMITHLSTASVLLAIAVSTLF
                     MMLVSGRFVPAMALVTASVTPRLRGSFLSFNISIQQLSAGIASLFAGSIMSTTPTGSI
                     MHYDTVGVIAAIATVLSILLAFQVHSLPE"
     gene            1924..2745
                     /locus_tag="PK522_09550"
     CDS             1924..2745
                     /locus_tag="PK522_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNC42015.1"
                     /translation="MCQKCMVTSDHCNSNYPASIVQDKHKDPTKREFIKRTSAVLMSW
                     GLLATGLVNASESKTVNEKKRIIPKPENVLTPDQALERLMQGNERYVSGNSKPLDFHD
                     VKTSLVKGQNPYATILGCSDSRVSPEHCFDEALGDLFVARGAGNYLTNDNLATIEYSV
                     AVLSTPLIMVLGHESCGAVKAAIDAVDRHRDFPGHIQLLASAIAPAVRTVSEIAKVSD
                     TTEDRLIHVTKQNVIMTVERLREKTPILDYYHDRKKLKIVGGIYHLDTGRVELIA"
     gene            complement(2799..3089)
                     /locus_tag="PK522_09555"
     CDS             complement(2799..3089)
                     /locus_tag="PK522_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42016.1"
                     /translation="MRRIILLMFMVLLLCGFRAPNGALISKGDLVDKLIINLGQPLAR
                     SPFVTQRGLYLPYHSGSRGYSVREVWIYKVEQYTYRFIIDNGVIVHEDWTRF"
     gene            complement(3218..4102)
                     /locus_tag="PK522_09560"
     CDS             complement(3218..4102)
                     /locus_tag="PK522_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNC42017.1"
                     /translation="MLISATFSLFNPEWFTPLNQGSTVVLILAFVMLCMGLTLTIDDF
                     RRIGRMPKAVAAGFLGQYTIMPVLAWGIAMLLNLPIHFAVGLILVGCCPGGTASNLVS
                     YIAKADVALSVVMTVCSTLAAIILTPLLTQLFAGTLVPVDTWMLFKQTLQVVILPIAL
                     GITLNHWLPSTVQRIMPLAPLLAVIGVCIICATVFAGQAEGIRHYIGELILATALLHA
                     GGFFLGYTLARYSNHDKQSMRTISIEVGMQNSGLAIVLSKQAFPLLPLAPVVGAISVL
                     MHSLLGSLLAVIWRSQKT"
     gene            complement(4157..4873)
                     /locus_tag="PK522_09565"
     CDS             complement(4157..4873)
                     /locus_tag="PK522_09565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC42018.1"
                     /translation="MSTNQPTTIFTQSWSLYDLITAYNYMFHQEIYEGVQKLLGLRQD
                     VTRYRLMDLGCGNARFMAPCLLKYPPSYYEGVDLSETALTEAKTYLAPLTCPVKLTHN
                     DLLTSIESTQDQWDIIFSGFAVHHLTPEDKTRFFNAVGRSLSKQGWLILVDVVREEHQ
                     DRETYLNHYLQFMRETWTKVPPEQLEEACTHVHDHDYPETFSTLQNMAIAAGLHTQRI
                     ISRYGQHYTLLFSPVNLPKI"
     gene            complement(4909..>5100)
                     /locus_tag="PK522_09570"
     CDS             complement(4909..>5100)
                     /locus_tag="PK522_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42019.1"
                     /translation="IITANASLAIGLNSIGAVLLASMGALFIFFSFRGVFPIQKYGKN
                     NCTALVLIIFDSEDKNTKE"
BASE COUNT         1628 a          970 c         1134 g         1368 t
ORIGIN      
        1 ataaagacta tgtccaaata ggaaaaattt catatgttgg agcaagtcgc tacgattttc
       61 ccaaaataat gttttccatt caaaattttt cagtaaacat gttaaatctt catggctact
      121 cgttactaaa ataccggact catcgagtag tgtaatagcg tctcttaatc ttccccgggc
      181 cgatcgaggc tgtttctcag ctatttttag cgtatggtag tgaagtgcat tgagttgcgc
      241 ttttgattga ggaaaagtca tccaaacgag tgcattgaaa agatcgtgcc aattctgtgc
      301 tcttgtctgg agttcaccgc ataaataggt tcttggctca tattgctgtt caaaaagcga
      361 tgtcggtgtg gtttgtgtta caaatcgcag cggatggtga gtattcgttc gaatatttga
      421 tttctgaacc gatagcaaat gattcaattg atcaggggtt ggccactgtt ctaattgatt
      481 aagtaattga ccagcctgac gcaaaggctc aaacatcggg gagcaatcaa ggaaatgatg
      541 tttccattga tcgatgtgtt cacgcttcat tcaggtaatg aatgaacctg gaaagcgagt
      601 aggatactaa gcacagtggc tatagctgca atgacaccca cagtatcgta atgcataatg
      661 gaacctgtcg gagtggtgct catgatagaa ccagcaaaaa gggaagcaat accggcagat
      721 agttgctgaa tcgaaatgtt gaagctaaga aaacttccac gtagtcgagg ggtaacactt
      781 gcggtaacta aagccatggc ggggacaaat ctgcctgaaa cgagcatcat gaaaagtgtt
      841 gatactgcta ttgccaatag aaccgaggcc gtactcagat gagtgatcat cagaattgga
      901 aacactgata atgcggctgc aatactaaat atcttatgct tgccgtgctg atcggagagt
      961 ttgccaaata accgtgccgt ataaaaagtt gctaatccac cgcagaaata aagataagga
     1021 atatcgactt cagacatgcc aatattggtt atcatatacg ttgtaatgaa aggactgacc
     1081 gaaaaacctg cgaacattag catggcaatc aatgcaaagg cgtataaatg attacggtta
     1141 taaaaaactg cgcgtaaatg ttgtgcagga tggcggtgtt ggcgatgctt gagatgaccg
     1201 tgtaaagaag gaagcaacca tattgccaag acaatcatta ccagactgat taatgttaga
     1261 aataagaagg gcgcacgcca actaaacaag ttcaccaata atatacccaa aggcattcca
     1321 ataaccgcta cagcagagaa ggctgacatg acagttccag tcgcatttcc ccgccgatgt
     1381 gccggggtca catcggcaac gatggtaaaa atggtggcgc taagtacgcc actaaaccca
     1441 cctgcgacca ttcgtgctaa taacaataaa taatactcaa acgagagtgc acatagcaat
     1501 gttgctaagc caataccacc gcagagaaaa ataaggaaac tttttcgatt gtaatggtcg
     1561 ataatgaatg aagcaaagaa actgaacgca cctgcactaa aagcgtaaat tgaaatcaat
     1621 aagctaaatt cttgaggtgt gagttcaaac aatttcatta attgcggacc aagcggcaat
     1681 aacatcatga aatccatgat atggataaaa tgaattcctg cgagtactgc aataatgggg
     1741 cgttcctgaa tgggtgtaaa atgaggaacg gtacctaatt caactaactc agagctttca
     1801 ccggtttgca gaatttgttc agtatcgtgc attttgtttt accggtttgt cagtgttatg
     1861 tgcaaagctg gaacaaataa taagatgtat tttaagttga tcagtaataa aggaattttg
     1921 aacatgtgcc agaagtgtat ggtaacgagc gatcattgta attcaaatta tccggcttca
     1981 attgtgcaag ataagcacaa agatccaact aaacgtgaat ttatcaaacg aacctcagct
     2041 gtactaatgt cttgggggtt gttggcaacc ggattagtta atgcttccga atcgaaaacg
     2101 gtgaatgaga aaaaaagaat catacctaaa ccagaaaatg tattaacacc ggatcaagca
     2161 ttagaaagat taatgcaagg caatgaacgt tatgtatccg gaaattctaa accattagat
     2221 ttccatgatg taaaaacctc attggtcaaa ggccaaaatc cttatgcaac cattctgggg
     2281 tgctctgatt ctagggtgag tcctgagcat tgcttcgatg aagcactggg tgatttattt
     2341 gtagcgcgcg gcgcaggtaa ttatctaacg aatgataatt tagcgaccat tgaatattcg
     2401 gttgcagtat taagtacacc attaatcatg gtgttggggc acgaaagctg cggtgctgtg
     2461 aaagcggcca ttgatgctgt agaccgtcac cgtgattttc caggtcatat tcaattgcta
     2521 gcaagtgcaa ttgcgcccgc agttagaaca gtcagcgaga tagcaaaagt tagcgatacg
     2581 acggaagatc gcttgattca cgttactaag caaaacgtaa tcatgacggt tgaacggctt
     2641 cgcgagaaaa cacctattct tgattattac catgatcgta aaaaactgaa aattgttggc
     2701 ggaatttatc atctcgacac aggaagagtg gaactcattg cttagcttag aatttcctta
     2761 gtgattgatt ctttactaaa cagtagttat tgctaatatc agaatcgagt ccagtcttca
     2821 tgaacaataa ccccattatc gatgataaag cgataagtgt attgttcaac tttgtagatc
     2881 caaacttccc gtacactata acctcgtgaa ccactatgat aaggtagata aaggccgcgt
     2941 tgcgttacaa atgggctacg agcaagtggt tgacctaagt taataatcag tttatcaact
     3001 aaatcgcctt tagaaattaa agctccatta ggggcgcgaa atccgcataa tagaagtacc
     3061 ataaacatca ataagattat ccttctcatg atagtctcct tgcaattggt tggttgatca
     3121 ctcgaactgt tattcaatcg ttagtaataa caatacgaga tgacccctga atgcaaactg
     3181 aataatctgg tttttttgaa gatactttgg agctgattta ggttttctgg cttcgccaga
     3241 tgacagcaag aagacttcct aataatgaat gcatcaaaac tgaaattgcg ccaactactg
     3301 gtgcaagcgg gagcagtgga aatgcctgtt tcgagagcac aatggctaaa ccggaattct
     3361 gcatacctac ttcgatcgaa atggtgcgca tactttgttt atcatgattt gaatatctgg
     3421 ccaaggtata acccaagaaa aaaccacctg catggagtag tgccgttgct aatatcagtt
     3481 caccaatata atgacggatt ccttctgctt gaccggcaaa aacagttgcg cagattatgc
     3541 acacaccgat aacggccaat agtggtgcaa gaggcatgat acgttgcaca gtgctaggga
     3601 gccagtgatt gagtgtgatg ccgagtgcga tcggtaaaat aacaacttgt agcgtttgtt
     3661 tgaataacat ccaagtatcc actggcacta aagtaccagc aaataattga gtgagtaggg
     3721 gtgtaagtat gatagcagcg agcgtcgaac aaacggtcat gacgactgaa agcgcaacgt
     3781 ctgctttggc aatatatgaa accaaatttg aagcggtacc ccctgggcag catccgacta
     3841 gaattaaccc taccgcaaaa tgaatcggca agtttagtaa cattgcgatt ccccaggcaa
     3901 gaacaggcat aatggtatat tgccccaaaa aaccagctgc aacggctttt ggcatgcggc
     3961 caattcgacg aaaatcatcg atggtaagcg tcaaccccat gcaaagcatt acaaaagcta
     4021 ggataagcac gacggttgat ccttgattga ggggtgtaaa ccattcaggg ttgaataatg
     4081 aaaaggtagc tgatatcagc atccacaatg ggaaaagcag tgttaatttt tgaagcatac
     4141 cgatgatgaa gtggaattaa atcttgggta aattcacagg agagaataac agggtgtagt
     4201 gttgcccata gcgactaata attctttgag tatggagtcc agcagcaatt gccatgtttt
     4261 gcaaggtaga aaaagtttca gggtagtcat gatcatgcac atgggtacaa gcttcttcaa
     4321 gttgttcggg tggaacttta gtccaagttt cccgcatgaa ttgtaaatag tgatttaagt
     4381 aggtttcgcg gtcttgatgt tcttcacgta cgacatcgac taagataagc catccttgtt
     4441 ttgacaagga tcggcccact gcattgaaaa atcgtgtttt atcctccggc gtaaggtgat
     4501 gaactgcaaa tccactaaaa atgatgtccc attgatcttg agttgattcg atcgatgtta
     4561 acaaatcatt atgggttaat ttaacgggac aagtaagtgg ggctagatag gttttagcct
     4621 cggtcagcgc tgtttctgaa agatcaaccc cttcataata gctgggtggg tatttcagta
     4681 aacagggtgc catgaagcgc gcatttccgc atccaagatc cattaaacga tagcgtgtaa
     4741 cgtcttgccg taatccaagc aatttttgta caccctcata gatttcttgg tgaaacatat
     4801 agttgtatgc agtaatgagg tcatacaacg accaagattg agtaaaaatg gttgtcggtt
     4861 ggttggtgct cataaaagtc aatttacaat aatcaggttt tagttcgcct attctttagt
     4921 gtttttatcc tcactgtcaa aaataattaa caccaacgcg gtacagttat ttttaccgta
     4981 tttttgaata gggaaaaccc ctctaaagga gaagaaaatg aatagcgcac ccatggaagc
     5041 aagcaataca gctcctatcg aatttaaacc aatagctaat gaggcatttg ctgtaatgat
//
LOCUS       DAOQLJ010000149         5080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_7578_length_5080_cov_5.773532, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000149 DAOQLJ010000000
VERSION     DAOQLJ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7578_length_5080_cov_5.773532"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(464..802)
                     /locus_tag="PK522_09575"
     CDS             complement(464..802)
                     /locus_tag="PK522_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42020.1"
                     /translation="MMRNQDISVAKAKKAVSDYKKAIGRPEGIAELTVFYCESCMDFL
                     GYCGMDDEGYFNALILMFEQALKAIQNLDVKQQAGFVERLNVLQHAGHNWGMGDEIED
                     LMVKYGFTKT"
     gene            complement(799..1008)
                     /locus_tag="PK522_09580"
     CDS             complement(799..1008)
                     /locus_tag="PK522_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42021.1"
                     /translation="MKKSGKKNPTWNDLKRQLANFDHTGLLCLIQDLYVADKQNQAFL
                     HARFGLVTMRSHRTKPLLIAGFVPM"
     gene            complement(1118..1381)
                     /locus_tag="PK522_09585"
     CDS             complement(1118..1381)
                     /locus_tag="PK522_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HNC42022.1"
                     /translation="MNWEIYYTKQAQKDALKISQAGLKPKVVELLKLLVENPLQNPPS
                     YEKLVGDLTGAYSRRITIQHRLIYQIYPEDKKIKVLRMWTHYE"
     gene            complement(1378..1620)
                     /locus_tag="PK522_09590"
     CDS             complement(1378..1620)
                     /locus_tag="PK522_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNC42023.1"
                     /translation="MTILKATEARTRLYNLIDEAAETHQPIVITGKRNNAVLISENDW
                     NAINETLYLVSIPGMRSSIREGMESNLSECSKEIDW"
     gene            complement(1779..2408)
                     /locus_tag="PK522_09595"
     CDS             complement(1779..2408)
                     /locus_tag="PK522_09595"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNC42024.1"
                     /translation="MNDWQNWRKQQRAELIAQRMQISSSDRILWNQAITHFLIQGFPI
                     LQQHAVGFYSAYKGEYDPMPVMEHLNACGTTLALPEVIESGQPLIYRKWWPQVSIKKD
                     RYGLSVPCDTEAVLISAVMIPMVGFDDQGYRLGYGSGYFDRTLAAMTTHPLTIGIAFE
                     QQRIATTYPQSHDIPMDFIVTERSIYALNQRLHPLSIENCARVSTQSQS"
     gene            complement(2418..3491)
                     /gene="mutY"
                     /locus_tag="PK522_09600"
     CDS             complement(2418..3491)
                     /gene="mutY"
                     /locus_tag="PK522_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNC42025.1"
                     /translation="MPNFANLLIRWQQQHGRHDLPWQGTQNPYLIWVAEIMLQQTQVK
                     TVIPFYQRFIETFPTIAALAKADLDDVLAMWSGLGYYARGRNLHKAAGVIMNDYQGKF
                     PTCSSEIQKLPGIGRTTAAAIAAFCYGEPCTILDGNVKRVLARYFGIEGYPGLNATQQ
                     RLWEKAEAVLPRGNTQKNMPAYTQALMDLGAIICTRTSPRCTACPLQSHCTAFLENRI
                     SCLPTPKPSKALPSKETVFLLLLHHDKLLLEKRVNQGIWKELWCLPEAEKNDHYQNYC
                     AQMLGIDAVCVDLLLPPIDHTFTHFKLRMYTQRLQVKSIIQQPQCNPIRWVTTDEAFQ
                     LAIPTAIKKILSKYRAASTPTSL"
     gene            complement(3525..>5080)
                     /locus_tag="PK522_09605"
     CDS             complement(3525..>5080)
                     /locus_tag="PK522_09605"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNC42026.1"
                     /translation="GNFSFQGDVKADGEPLKIALQGKAQLANFNVLDRVKLAEQLRWK
                     SIDVTGIQFINEPLQIGMTTIDLTDLFARVILLADGKINLRDLTEANKAEASPVKPES
                     TPVASAPNQAEAQGAANNKAPLPIKIDKVTLKNTHINFTDQFIKPNYRVNLTELKGQV
                     SPLHPGKAGKVDIRGTIDKSAPLQISGTIDPFSEQLSFDIATTVKGIDLPTFSPYSGK
                     YIGHLIEKGKLSVDVNYQIQQGQLSAENKIFLDQLKIGEKVDSPDAVSLPLDLAISLL
                     KNRKGEINLRFPVSGSINDPQFSISGVVWEAFINLISKALTAPFALLGSFLGDGEELA
                     EITFPSGYATLEPEAINHLQSLADALIDRPELKLEITGYADPLNDYETLKQVLLDRKV
                     KTQKLSETVNKGQSVDLLDEIKLDDAEYARYLEIVYKASDFEKPKNMIGLSKSLPVDE
                     MKQLILTNTKVSDEALNELAEKRASVVFHWLIEQGGVPSERVFVLGTHLESASAAEKP
                     YSKAAFALK"
BASE COUNT         1377 a         1067 c         1105 g         1531 t
ORIGIN      
        1 gggatgaaat tgaggaagga atacaaatgg gctatgaagc tgcaaattct cctgttgaac
       61 tatcattccc ggtaccagat cattcaataa gataagtgta atggtctaca aaaactgaaa
      121 atctactatt tgacagttaa ttataaaata gtaccggtct cgcgatggga tatgatccag
      181 aaatcaataa aatctcaggt ccattgatcc tattgctgca gatcagcaca aaaatattag
      241 cgttacaata gttaatcata ttagcgcagt aagcaaaaaa actgtccaca aataaatggg
      301 gtctggtctt gtattaacat aaagagaatt ttgcaaaaca cctgacttca taaaatcgag
      361 caaagatagt gcgattccag tttgatttac tcaatttttt atgaatttct ataaaaaaca
      421 caatgaaaat aatcaaactg tatgggacaa ctccaaactc catttacgtt ttggtaaatc
      481 catacttcac catcaagtcc tctatttcat ctcccatgcc ccagttgtgc cctgcgtgct
      541 gaagaacatt gagtcgttca acaaaacccg cttgctgttt gacatcaaga ttttgaatcg
      601 cttttaaagc ctgctcaaac atgagaatca aggcgttgaa atagccttcg tcatccattc
      661 cacagtagcc gagaaaatcc atgcaagatt cgcaataaaa tactgtcagc tcggcgatcc
      721 cctctggtcg cccgatggct tttttgtaat cggagaccgc tttcttggct ttggcaaccg
      781 atatatcttg attgcgcatc acatcgggac aaatccagca atcaataatg gttttgtacg
      841 gtgcgagcgc atcgtcacca agccaaagcg agcatgtaga aaagcctgat tctgcttgtc
      901 agcaacgtag agatcctgaa tcagacagag gagtcctgtg tggtcgaaat ttgcaagttg
      961 tcgtttgagg tcattccacg taggattctt cttgccggat tttttcatta tattttgcta
     1021 cttacttttg gtgattctaa aggctaagtt atcgatcagt tgtgttaaat gctgcggata
     1081 atcatatcaa taaacttagt cttgttgaat caagttttta ttcataatgc gtccacattc
     1141 gcaacacttt gatcttctta tcttctggat atatctggta tatcagccgg tgttgaatgg
     1201 taattcttct cgaatatgca cccgttagat ctccaaccag cttttcatag gaggggggat
     1261 tctggagcgg attttcaacg agtaatttca atagctctac aactttaggt ttcaatccag
     1321 cctgactaat tttcagtgca tctttctgag cctgtttggt gtaatagatt tcccagttca
     1381 ccagtcgatt tccttggagc actccgataa attggattcc atgccctctc gaatagacga
     1441 tcgcatgccg ggaatcgata ctagatacag tgtttcgttg atcgcgttcc aatcgttttc
     1501 agatatcaat accgcattat ttcttttgcc cgtaataaca attggttgat gcgtttcagc
     1561 cgcttcatca attaagttgt aaagccgagt gcgggcctca gtagctttga gtatggtcat
     1621 gatagcttcc tcatttttta tgaatataat cgtacgtcaa tgcgtacgat agagcaagaa
     1681 atattcagct gagaaatttc aatttttctt caaaataaca cgtacagatt tactagttaa
     1741 tttctaagtt tgtggtttat aactgtggat tctggaggct aactttgaga ctgcgtacta
     1801 acacgcgcac agttttctat cgacaaagga tgcaatcgct gattcagggc atagatcgat
     1861 ctttcggtta cgataaaatc catcggaata tcgtgggatt gcggataagt cgtggcaatt
     1921 cgctgttgtt cgaacgcaat gccaatcgtt aacgggtgag ttgtcatagc agcgagagta
     1981 cgatcaaaat aaccactgcc atagcctaag cgatagcctt gatcgtcaaa ccccaccatc
     2041 ggtatcatca cagcactaat cagcactgct tcagtatcgc agggaacaga taaaccgtac
     2101 cgatcttttt tgatggaaac ctgtggccac cattttctgt aaatgagggg ctgacctgac
     2161 tcgatcactt caggaagtgc gagggtagtg ccacaagcat ttagatgttc catgacgggc
     2221 atgggatcat attcgccttt gtaggctgaa taaaagccaa ctgcgtgttg ttgcaaaatt
     2281 gggaaacctt gtattaggaa atgagtaatc gcctgattcc aaaggatacg gtcgctgcta
     2341 cttatttgca ttcgttgtgc aataagctca gcacgttgct gtttccgcca gttttgccaa
     2401 tcattcatag tcttgatcta aagggatgtt ggtgttgatg ctgcacggta ttttgacaga
     2461 atctttttaa tcgcagtcgg tatggccaat tgaaatgctt catcggttgt aacccagcgt
     2521 attggattgc attgcggttg ctgaataatg gatttgactt gcagccgttg cgtatacatc
     2581 ctgagcttaa aatgggtgaa cgtgtgatca atcggtggta acaataaatc gacacatact
     2641 gcatcgattc ctagcatttg cgcgcagtag ttttgatagt gatcattctt ttctgcctcg
     2701 ggcaaacacc ataattcctt ccaaatacct tgattcactc gtttttctag caatagcttg
     2761 tcatggtgca gcagcaatag aaaaactgtt tctttgcttg gtaaagcttt ggaaggttta
     2821 ggcgttggca gacaagagat tcgattttcc agaaatgcgg tgcaatggga ttgcaaaggg
     2881 caagctgtac aacgtgggga agtgcgcgtg cagatgatag cgcccaaatc catgagcgcc
     2941 tgagtataag ctggcatatt tttttgagta ttaccacgcg gcagaacggc ttcagctttt
     3001 tcccaaagcc gctgttgcgt ggcgtttaat ccaggataac cttcgatacc aaaataacgt
     3061 gcaagtacgc gtttgacgtt accatcgagg atggtgcagg gttcgccata acaaaatgct
     3121 gcaatcgcgg cggctgtcgt tcgaccgata cctggcaatt tttgtatttc ggacgaacac
     3181 gttggaaatt tcccttgata atcattcatg atgacacccg cagctttatg aagattgcgc
     3241 ccgcgtgcgt aatagccaag gccgctccac atagctagca catcatccag gtctgctttg
     3301 gcaagcgcag caatggtagg aaaagtttct ataaaacgtt gataaaatgg aataaccgtt
     3361 tttacctgag tctgctgtag cataatttct gcgacccata tcagataagg gttctgagtg
     3421 ccttgccatg gtaggtcatg cctaccatgc tgctgttgcc agcgaatgag caggtttgca
     3481 aaattaggca tgccttgcag gatggataag tgattaccgg gagattattt gagcgcgaat
     3541 gcagctttgc tataaggttt ctcagcagca cttgcagatt ctaaatgagt tccgagcaca
     3601 aaaacccgtt cactcggcac accaccttgc tcaatgagcc aatgaaatac cacgcttgcc
     3661 cttttctctg ctaactcatt gagcgcttca tcactcacct tggtattcgt taaaatgagt
     3721 tgcttcattt catcgactgg caagcttttg cttaatccaa tcatattttt gggtttctca
     3781 aaatcagatg ctttgtaaac gatttctagg taacgtgcgt attcagcatc gtctagtttg
     3841 atttcatcga gtagatcgac ggattgccct ttattaaccg tttctgaaag tttttgtgtt
     3901 ttgactttac gatcgagcaa tacttgcttg agggtttcat aatcgttgag ggggtcagca
     3961 tagcccgtga tttcgagctt gagctcgggt cgatcgatta aagcatcggc caaactctgg
     4021 aggtgattga ttgcttcagg ttcaagcgtg gcatagcctg aaggaaaggt gatttccgct
     4081 aattcctctc catcacctaa aaaagaaccc aataaggcaa agggtgccgt caaggctttc
     4141 gatatcaagt tgataaaggc ctcccaaact actccgctga tgctgaattg aggatcattg
     4201 atcgagccac tgactgggaa gcgcagattg atttcccctt tgcgattttt taatagggat
     4261 atcgctaaat ccaaaggcaa tgaaacggca tcagggctat cgactttctc gccaatcttt
     4321 agttgatcga gaaatatctt attctcagca cttaattgac cttgctgaat ttgataattg
     4381 acatcaaccg aaagcttgcc cttttcaatg agatgtccga tgtatttacc cgaataaggg
     4441 ctgaaagttg gtaaatcgat ccctttcacc gttgtcgcga tatcaaagga aagctgttca
     4501 ctaaaaggat cgatagttcc tgagatttgc agcggtgctg atttgtcgat cgtacctcga
     4561 atatcgactt ttccagcttt tccgggatgg agtgggctga cttgaccttt aagctcggta
     4621 aggtttactc ggtaattagg tttgatgaat tgatcggtaa agttgatatg ggtatttttt
     4681 aaggtgactt tatcaatttt gatcggcaaa ggggctttat tgttagcagc accctgtgct
     4741 tctgcctgat ttggtgcact cgcaactggt gtggattcag gttttactgg actggcttca
     4801 gctttatttg cctcggtgag atctcgcaga ttaatctttc catcggctaa aagaatgacg
     4861 cgcgcaaaaa gatccgttaa atcgatcgta gtcattccaa tttgcaaagg ctcgttgata
     4921 aattgaatgc cggtcacatc gatacttttc caacgtaact gctcagcaag tttgactcta
     4981 tcgagcacat taaaattggc gagctgcgct ttaccctgca aagcaatttt taatggttcc
     5041 ccatcagctt taacatcgcc ttgaaacgaa aaattacctt
//
LOCUS       DAOQLJ010000150         5068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_7613_length_5068_cov_5.910632, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000150 DAOQLJ010000000
VERSION     DAOQLJ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7613_length_5068_cov_5.910632"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1748)
                     /locus_tag="PK522_09610"
     CDS             complement(<1..1748)
                     /locus_tag="PK522_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNC42027.1"
                     /translation="MFKKILIANRGAIACRIMRTLRRLNVASIAVYTEADHLSRHVDE
                     SDEAYCIGNGAVAESYLDINKILAIAKQAQAEAIHPGYGFLSENPNFAEACAQQGIRF
                     IGPTPEQIRIFGLKHTARSIAQQNQVPLLPGTDLLSDVEAACRHAHTIGYPVMLKSTA
                     GGGGIGMRLCADEAELINHYEAVKKLAASHFKESGLFLEKYIAQARHIEVQIFGDGKG
                     GVIALGERDCSLQRRNQKVVEETPAPNLSPAIRSALLNTAVKLGQAIHYESAGTVEYI
                     FDTQTQQFYFLEVNTRLQVEHGITEAVTGVDLVEWMVKQAAGELPPLTAFTIKPQGVA
                     IQARIYAENAAKNFQPCCGTLTTVEFPQTCRVETWVENGTELSAFYDPMIAKMIVHAP
                     TRNAAVNKLITALNETSLYGIETNLSYLKQIISSDVFQKGQVSTQFLSQFTYLASTLD
                     VLDPGTQTAVQDYPGRLGYWNVGVPPSGPMDSLAFRIANRLVNNPEHLAGLEITLNGP
                     TLRFNCASVIALCGAPIEALLDNEPLALWQSHAVRAGTLLKISKIDSVGSRVYLAVQG
                     GIQVPHYLGSRSTFMLG"
     gene            complement(1788..2459)
                     /locus_tag="PK522_09615"
     CDS             complement(1788..2459)
                     /locus_tag="PK522_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HNC42028.1"
                     /translation="MSHQQYIESYLTPDTAIIDEICPAGEPWSKVIQHGQIFRIVDLE
                     GNQAVDTLFFNAHAPQERYSAADTIRNQSNVYLTTNSQLYSNVGNILLTIIADTCGRH
                     DTLGGACAAESNTVRYALEKFPMHSCRDNFLYALATEPIFNQLGMNKRDLPSNINFFM
                     NVPVTESGDLTFSDGVSAPGKYVEMRAEMDVLVLISNCPQLNNPCNAYNPTPIRLLVW
                     DSPCA"
     gene            complement(2456..3184)
                     /locus_tag="PK522_09620"
     CDS             complement(2456..3184)
                     /locus_tag="PK522_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HNC42029.1"
                     /translation="MEHNDIDWKLIIPGGCHWSGILRRGTTLRLTDIDGNANVAALFY
                     NQEEKLERYNMADTLKAQHTFYLTQGHACYSDMGRIFCCITADTAGWHDTVCGLSNAK
                     LIEQKYGTARYQAHRNQMFRSGLDGILIELGKWGLGLRDVVANINFFSKVTANQEGQL
                     TFQRDHRSPNASVDLSFEMNTLVVLSMAPHPLDTALTYAPGPVQLSIFPTQPTTQWPC
                     SQRPENIRGRHNTQQFYCLHGGTQ"
     gene            complement(3171..4613)
                     /locus_tag="PK522_09625"
     CDS             complement(3171..4613)
                     /locus_tag="PK522_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNC42030.1"
                     /translation="MEHSQKSKQPLPSASINPGETLNRNMGIWNSFTLGFAVVSPVVG
                     LYAIVGVQAQVTGGGWFAALIICLAMQLFVATVYAELASQFPIAGGAYKWAHLLCGRI
                     TGQYAGAIYVSSTIAMLTTTAYTGGIWLAILFGLNSESGIAMVGWGIAFLLLCLAINL
                     APVKFFKGIIAMGVYAEIVGSFGMALLLFLFFRELPFSELFQHLGTGTAPNQLSAFLA
                     ALAIAGWAFIGFDACSTTSEETHQPKHSVPRAVFLSLLAVGTVVVFNSAALTLSVDHE
                     VLSKTSSTFDPITPLISTHIGAWAEKPFLLIVVIAFLACGASVVKYTSRIVFSMAREE
                     ILPSQLSIVSHHGTPHHAILATVVFSSMGLMLGLSDEAVATIISFGTGGLYAMFATTT
                     GVALFSRLTGRWNPALGELKLGIWGLIINSIAFLWSVFELINIAWPRSYMAAPDAPWW
                     QLWAVPLVLAGILVLTTLVILIKGEKHGTQ"
     gene            complement(4618..4800)
                     /locus_tag="PK522_09630"
     CDS             complement(4618..4800)
                     /locus_tag="PK522_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42031.1"
                     /translation="MPSHEKHVIFFAVSSVTEPNTDKKSRYPTQIPIRESESVRCIFY
                     VIVISLELWFFYIIEE"
     regulatory      complement(4857..4968)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
BASE COUNT         1364 a         1136 c         1250 g         1318 t
ORIGIN      
        1 cctaacatga atgtggagcg gctaccgagg taatggggta cttgaatacc cccttgtact
       61 gcgagataaa cgcgactgcc gacagagtca attttactaa ttttcagcaa ggtgccagcg
      121 cgaacagcgt gcgattgcca tagcgcaagt ggttcattat cgagcaaggc ctcgatcggt
      181 gcgccacaga gcgcaattac gctagcgcaa ttaaagcgca gtgtcggacc gttcagcgtg
      241 atttcaagcc cggctaaatg ttcagggttg ttgaccaatc gattggctat ccggaaggcc
      301 aatgagtcca tcggaccaga gggcgggaca ccgacattcc aatatccgag cctgccgggg
      361 taatcttgta ccgctgtttg cgtgcctgga tcaagcacat cgagggtgct ggctaaatag
      421 gtaaactgac ttagaaattg cgtactgact tgacctttct gaaacacatc actgctgatg
      481 atttgtttga gataactgag attggtttca ataccatata aggaagtttc attaagcgcg
      541 gttattaatt tgttgactgc tgcattccgg gttggtgcat gcacgatcat ttttgcgatc
      601 atgggatcgt agaaagcaga tagctcggta ccattttcaa cccaggtttc aacccggcag
      661 gtttggggaa attcaaccgt tgtcagggtg ccacaacacg gttgaaaatt tttagccgca
      721 ttctcagcat aaattcttgc ctgaatggca acgccttggg gtttaatcgt gaaagcggtg
      781 agtggtggca gttctcccgc tgcctgtttg accatccatt cgacgaggtc caccccagtc
      841 acggcttcag tgatgccgtg ttctacttgt aaacgcgtgt tgacttcgag gaaataaaat
      901 tgctgagttt gagtatcaaa gatatattca acggtgccgg ctgattcata gtgtatggct
      961 tgaccgagtt taaccgcagt atttaatagc gctgaacgga tcgccggtga aaggttagga
     1021 gcaggtgttt cttcgaccac tttctggttg cgacgctgta acgagcaatc gcgctcaccg
     1081 agtgcgataa caccgccttt tccatcacca aagatttgca cttcaatgtg gcgagcttgg
     1141 gcaatgtatt tttcaaggaa aagccctgat tccttgaaat ggctagctgc cagcttttta
     1201 accgcttcat agtggttaat taattcggct tcatctgcgc atagccgcat cccgataccg
     1261 ccgccgccag cggtactttt gagcatcact gggtagccaa tcgtgtgggc atgacgacag
     1321 gccgcttcaa catcggataa caaatcagta ccaggcaata gtggcacttg gttttgttgc
     1381 gcaatgctgc gtgcggtatg ctttaaccca aaaattcgaa tttgctccgg tgtcgggccg
     1441 ataaagcgga tgccctgttg tgcacaggct tcagcaaaat tagggttttc actgaggaaa
     1501 ccataaccgg ggtgaatcgc ttcagcctgg gcttgctttg caatcgctaa aattttgtta
     1561 atatccaaat aactttcagc aaccgcaccg ttgccaatgc aataggcttc gtcgctttca
     1621 tcgacgtggc gcgataaatg gtctgcctcg gtataaaccg cgatactggc gacgttcaat
     1681 cgacgtaacg tacgcatgat gcgacaggca atggcacccc ggttggcaat gagaattttt
     1741 ttaaacatga cgattcttcc tgtgaatgat ggcgtagtta acttggctta agcgcaaggc
     1801 gaatcccaaa cgagcaagcg aatcggtgtc gggttatagg cattgcaggg gttgtttaat
     1861 tgagggcaat tcgatatcaa caccaacaca tccatttcag cacgcatttc gacatacttg
     1921 cccggtgctg aaactccatc gctaaacgtt agatcaccgg attcggtcac cgggacattc
     1981 atgaaaaaat tgatattgct cggcaagtcg cgcttattca tccccagttg attaaaaatg
     2041 ggttcagttg ccagcgcata taagaaatta tcgcggcaac tgtgcatcgg aaatttttct
     2101 aacgcatagc gaaccgtatt actttctgca gcgcaagcgc caccgagggt atcgtggcga
     2161 ccacaggtat cggcgataat ggttagtagg atgtttccaa cattggaata gagctgactg
     2221 tttgtcgtga gatatacatt cgattggttg cgaatggtgt cagcggcact gtagcgttct
     2281 tgtggtgcat gggcattaaa aaataaggtg tcgactgcct gattaccttc taaatcgacg
     2341 atacggaaaa tttgaccgtg ttgaatgacc ttactccacg gttctcctgc ggggcaaatt
     2401 tcatcgataa tcgctgtgtc aggcgttaaa taactttcga tgtattgttg atggctcatt
     2461 gtgttcctcc gtgcaagcaa tagaattgct gggtattgtg tcgaccccta atattttcag
     2521 gacgctgtga gcagggccac tgggttgtcg gttgtgtggg gaatatcgat aattggaccg
     2581 gcccaggcgc ataagtgagt gctgtatcga gaggatgcgg tgccatcgaa agcacgacta
     2641 aggtattcat ttcaaaactc agatcaaccg atgcgttggg ggaacggtgg tcacgctgaa
     2701 aagtgagttg accctcttga ttggcagtga ctttactgaa aaagttgatg ttggcaacga
     2761 catcccgcaa accaaggccc catttgccaa gttcaattaa tattccatcc aacccactgc
     2821 gaaacatctg attgcgatgc gcttgatagc gagcggtacc gtatttctgc tcaataagct
     2881 tggcattact tagcccacaa accgtatcgt gccatcctgc cgtatccgct gtgatacagc
     2941 aaaaaattct tcccatatcg gagtagcagg catggccttg cgttagataa aaggtatgct
     3001 gagcttttaa agtatctgcc atgttgtaac gctcgagttt ttcttcttga ttatagaaga
     3061 gtgctgctac gttagcattt ccatcgatat cggttaatct tagcgtagtg ccacggcgca
     3121 agatgcctga ccaatggcag cctcctggaa tgattaattt ccaatcgatg tcattgtgtt
     3181 ccatgttttt ctccttttat caagataact aaggttgtta gcaccagaat gccagctaaa
     3241 acgagcggta ctgcccagag ttgccaccaa ggggcatcag gtgcagccat atatgagcgt
     3301 ggccaagcaa tattgatgag ttcaaaaaca gaccataaga aggcgatact gttaatgatt
     3361 aaaccccaaa tccctaattt gagttcccct aaagcaggat tccaacgtcc cgtgaggcga
     3421 ctgaataaag caacgccggt tgtcgttgca aacatcgcat ataatccgcc agtgccgaat
     3481 gaaataatcg ttgcaacggc ctcatcgctg agtccaagca ttaagcccat actggaaaat
     3541 actacagtgg ctaggatagc gtgatggggt gtgccgtgat gagacactat gctgagttgg
     3601 gaaggtaata tttcttcacg ggccattgag aatacgatgc gcgaggtata tttgacaacg
     3661 gatgcgccac aagctaagaa agcgataacg acgatcagca aaaagggttt ctcagcccag
     3721 gcgccgatgt gagttgagat gagcggtgta atcggatcaa aggttgaact ggttttagaa
     3781 agcacttcat gatccaccga taaagttaat gcggcagaat taaaaactac tacggtgcca
     3841 acggcaagga gggagaggaa cacggcccgg gggacggaat gtttaggctg atgagtttct
     3901 tccgatgtgg ttgaacatgc atcaaagcca atgaatgccc accccgctat tgctaaagct
     3961 gctaaaaaag cggatagctg attgggtgcg gtgcccgttc ctaagtgctg gaaaagctca
     4021 gaaaagggta gctctctaaa gaatagaaat agtagtagtg ccattccaaa agagccgacg
     4081 atttctgcgt agacgcccat ggcgatgatg cctttaaaaa atttaactgg cgccaggttg
     4141 attgcaaggc acagcaataa aaaggcaata ccccacccga ccatcgcaat acctgattcg
     4201 ctgtttaagc caaagaggat cgctaaccag attccacccg tataagctgt ggtcgttaac
     4261 atcgcgatag ttgagctgac ataaatggca cctgcgtatt ggccagtgat ccgaccgcac
     4321 agtaaatgag cccatttata ggcgccaccg gcaattggaa attgcgaggc gagttcggca
     4381 taaacggttg ccacaaataa ttgcatagca agacagatta taagtgctgc gaaccaacca
     4441 ccaccggtta cttgcgcttg cacgcctacg attgcataaa gcccaacgac gggagatacc
     4501 acggcaaaac cgagtgtaaa gctattccag atacccatat ttcggttgag tgtttcgccg
     4561 ggattgatgg aagcgctagg caatggttgt ttagactttt gtgaatgttc cattgcatta
     4621 ctcctcaatg atgtagaaaa accagagctc cagggagatt acgatgacgt aaaaaatgca
     4681 acgcaccgat tctgattctc gtattggaat ctgagtcgga taacgtgact ttttgtcggt
     4741 attcggttct gttactgatg aaactgcaaa gaaaattaca tgcttttcat gtgatggcat
     4801 aactaaagct ccatttaaat aaaatacaga gagtcgtaat gtgtaaaaca cttgcgatct
     4861 cccgggcttt tatccctccg tggagcttcg ttatgaacga agccagaaac tctcggacca
     4921 gcacttcttc ctttatcgaa agtagtcgga accctagttt gctataacac caatcagtaa
     4981 ggtttgatgc actgattgga ttttaaggtc gtctctctaa aaaactattc gatgcaatgg
     5041 tttaggattt actcatatta accacatg
//
LOCUS       DAOQLJ010000151         5044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_7671_length_5044_cov_5.623572, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000151 DAOQLJ010000000
VERSION     DAOQLJ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7671_length_5044_cov_5.623572"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PK522_09635"
     CDS             <1..588
                     /locus_tag="PK522_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNC42032.1"
                     /translation="PLACEISVILARGFDGKTVHFPVIENQHQQGILDVSLVPARVSD
                     SIQQQACRIATELATKLDYQGVLCVEFFLLQDGQLLINEIAPRPHNSGHFSIDACITS
                     QFEQQVRTLCQLPLGSTTMKTPAAVMINLLGDLWHSGEPDWNNVLEQPAAKLHLYGKS
                     EARPGRKMGHITVLGDTLEHALDKALTIRATLSER"
     gene            590..1480
                     /locus_tag="PK522_09640"
     CDS             590..1480
                     /locus_tag="PK522_09640"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNC42033.1"
                     /translation="MKNSTALFETTLNSLPLIHRGKVRDIYAVDADNLLIIQTDRLSA
                     FDVILPTPIPGKGKLLTQISAFWFEKLKHIIPNHLTSVLPQTVVSPQECDQVIDRAFV
                     VKRLQPLPIEAIVRGYIIGSGWKDYQNSGAISGIKLPPGLREAEKLTQGAIFTPSTKA
                     QAGEHDENISFAICEQMLGTQLASQVRDISIALYTEAADYALQKGIIIADTKFEFGQD
                     QAGKLYLIDEVLTPDSSRFWPVDQYQPGMSPPSFDKQFVRNWLEQSGWNKKPPAPEIP
                     DEILLQTVNRYQEALIRLTQ"
     gene            complement(1482..2285)
                     /locus_tag="PK522_09645"
     CDS             complement(1482..2285)
                     /locus_tag="PK522_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNC42034.1"
                     /translation="MEWWLIYLLLGAIVGYIAGLFGIGGGLVIVPALVYMFTLQAFPA
                     DRVLHFALGTTMATIIFTSLSSLRMHHVHGAVNWLIVRYLSPGIILGTLVGSTLASTL
                     STSVLGVIVVLFIYYAATQMWLKLLPKPEYTLPGKGGLFMAGGIIGSVSSLVAIGGGL
                     LTVPFLSLCKVKLQQAIGTAAAVGFPIALAGTIGYIINGELQTQALPEYSLGYVYLPA
                     LGWIAAASMLTAPLGAKTTHRIQTATLKKLFIVLLYCLGTKMLFDILSN"
     gene            complement(2300..2755)
                     /locus_tag="PK522_09650"
     CDS             complement(2300..2755)
                     /locus_tag="PK522_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNC42035.1"
                     /translation="MRYWLMKSEPSEVSIDDLAAKPDQTVAWDGVRNYQARNFMRNQM
                     QLGDKTFFYHSSCEQPGIVGITTVSKLAYPDATQFNPKSKYYDPKSTPENPRWFNVEV
                     KLVRKTRLISLKELRSYPALANMRILQKGNRLSITPIDPAEWAFILGKI"
     gene            2829..3281
                     /locus_tag="PK522_09655"
     CDS             2829..3281
                     /locus_tag="PK522_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNC42036.1"
                     /translation="MPKLLFLSLLLFIVSNANSQIYKWIDEKGRVEYSDQPPPAGSPI
                     VPTRVSLQYSSSYSMDDENTPAPEHVTQKEEGFKERRAAREETKKKQLAEANTKKEQC
                     NQARSNMEILKNTPQLSIPDGMGGVKEADDNFRLKQMEDVKKMIASLC"
     gene            3548..>5044
                     /locus_tag="PK522_09660"
     CDS             3548..>5044
                     /locus_tag="PK522_09660"
                     /inference="COORDINATES: protein motif:HMM:NF040127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HNC42037.1"
                     /translation="MIHSFKFIKAAQVTFTLLLVFMVLIARNSYGFQSSPIVWTTTGI
                     WHATPEESCHYWASRRDIEYVGIPSSKYAEIKAMWVIPWGWNENNVLVMNIIPAVQYQ
                     CGARVFHYGNPPYQQPYEYDWRFGGAFPVCRVGDSPSVHSDTVCNIPDHIPDRGANLG
                     QPSTCVGNPINTATGNKFAQQIDVVLPTFTFTRFYNSFGFSSNGQIGASWRHNFQSSL
                     FINPTTITATRADGKLIYFRLEGAVWINQQSSGEALAPITDVSGSEGGWLLTTADDHK
                     KYYDTAGKLVAISDRAGRRVSLNYDTHYQLATVTDDVGRTLQFTYDGNNRIKALIDPS
                     GEQYQYHYDNQGNLTRVTYPDNHFISYHYNEPAYTSGANLPFALTGITDENGNRFAIY
                     HYDAQGRAIVTEHTDGADRYHLGYNPDGSTTITDSLNTQRTQTFQTLFGVVKMVSQSQ
                     PAGSGCDASSSDISYDANGNVASRTDFNGNKTCFSYDLDRNLEIARVDG"
BASE COUNT         1486 a         1085 c         1095 g         1378 t
ORIGIN      
        1 ccacttgctt gcgaaatttc ggttatcttg gcacggggat tcgatggcaa gacggttcat
       61 ttcccagtta tcgagaatca gcatcagcag ggtatactcg atgttagctt ggtgcccgcc
      121 cgtgtttcag attcgattca acagcaagcc tgtcgcattg ctactgagct agcgaccaaa
      181 ctggattatc aaggcgtgct gtgcgttgaa tttttcttat tgcaagatgg tcagttgctc
      241 attaacgaga ttgcgcctcg cccccataat agtggtcatt tttcaatcga cgcttgcatc
      301 acttctcaat ttgaacaaca agttcgcaca ctgtgccagt taccgctcgg cagtactaca
      361 atgaaaacac ccgctgctgt tatgataaat ttactgggag atttatggca tagcggtgag
      421 cctgattgga acaatgtttt agaacaacct gctgcgaaac tccatttata tggcaaaagt
      481 gaagcgaggc cagggagaaa aatggggcat attacggttc ttggtgatac gctcgagcat
      541 gcattagaca aagcattaac catcagggct actttatcgg aaaggtaaga tgaaaaattc
      601 gacagcactc tttgagacga ctctgaacag ccttcccctc attcatcggg gtaaggtgcg
      661 agatatttat gcggtggatg cagataactt gttgattatc caaaccgatc gattgtccgc
      721 attcgatgtg attctaccca cacctattcc gggtaaaggc aaattgctaa ctcagatttc
      781 agccttctgg tttgagaagc tcaagcacat tattcctaat catttaacca gtgttttgcc
      841 acaaacggtg gtatctccgc aagaatgtga tcaagtcatc gatcgcgcgt tcgtggtaaa
      901 acgtcttcaa ccgcttccga ttgaagcaat cgttcggggc tatatcattg gttctggctg
      961 gaaagattat caaaatagtg gagcaatcag cgggattaag ctaccgccag gattaagaga
     1021 agctgaaaag ctaacgcaag gtgcaatctt tacgccttca accaaagcac aggcaggaga
     1081 gcacgatgaa aatatttcgt ttgcgatttg tgagcaaatg ctgggaacac agttggcttc
     1141 acaggtgaga gatatctcca tcgcccttta tactgaggct gctgattatg cgttgcaaaa
     1201 aggaatcatc attgcagata ccaagtttga atttgggcaa gatcaagctg gtaagcttta
     1261 tttgatcgat gaagtattga ctccggattc atcgcgtttc tggccagtag accaatatca
     1321 accggggatg agccccccca gttttgataa gcagttcgtt cgaaactggc tagagcaatc
     1381 gggatggaac aaaaagcccc ctgcaccgga aattcctgat gagatcttgt tgcagactgt
     1441 taatcgttat caggaagcct tgattcgctt aacgcaataa tttaattgga taagatatcg
     1501 aacagcattt ttgtgcctaa acaatagagc aatacgataa atagcttttt tagcgtcgct
     1561 gtttggatac ggtgtgtcgt tttagctccc aatggcgctg ttagcatact ggctgctgca
     1621 atccaaccca aagctggtaa ataaacatac cctaagctat attctggaag cgcttgggtt
     1681 tgtagttcac cattgatgat ataaccgatg gttcccgcca gtgcaatcgg aaaaccaact
     1741 gctgcagcgg ttccaatggc ttgctggagc ttgactttgc atagcgaaag aaatggaacc
     1801 gttaaaagcc caccaccgat tgccaccaaa cttgataccg aaccgatgat acctcctgcc
     1861 atgaataacc caccttttcc gggtagcgta tattcaggct tgggtagtaa cttcaaccac
     1921 atctgggttg ctgcataata aataaagagt acgacgataa cacctagtac cgatgtcgat
     1981 aatgtgctgg caagagttga tcctaccaac gttcccaaaa taataccggg tgaaagatac
     2041 ctgacgatca accaattaac agcaccatgc acatggtgca tccgtaggct tgatagcgat
     2101 gtaaaaataa tcgttgccat agttgtaccc aaggcaaaat gtaaaactcg atcagcagga
     2161 aaagcttgta gcgtaaacat ataaaccagc gctggaacga tgacaaggcc accgccaatg
     2221 ccaaataatc ctgcgatata accaacgatt gcgccaagca ataaataaat caaccaccat
     2281 tccatcttaa ttctctgagt taaattttcc caaggatgaa tgcccactcg gcaggatcaa
     2341 tgggtgtaat cgataagcga ttgccttttt gcagtattcg catattagcg agcgctggat
     2401 agcttcttaa ttctttgagg gaaattaatc gggtttttct gacgagcttt acctcaacat
     2461 taaaccaacg aggattctca ggtgtcgact tgggatcgta atatttactc ttcgggttga
     2521 attgggttgc atcagggtaa gcgagtttcg acacggttgt aataccaaca atacctggct
     2581 gttcgcagct tgaatggtag aaaaaagttt tatctcccaa ttgcatttgg ttgcgcatga
     2641 aattacgtgc ttggtaattg cgcaccccgt cccaagcaac ggtttggtct ggctttgcag
     2701 ccaaatcatc aatactaact tcggaaggct ctgatttcat tagccaatac cgcatttatt
     2761 tatctccttc tatctcgata aagtttaaaa gaaaacgatc gatagatttt tattttagga
     2821 gaaaaaccat gcctaagctt ctttttctat cgctattgct ctttattgtt tcaaatgcaa
     2881 atagtcaaat ctataaatgg atcgatgaaa aaggccgcgt ggaatactcc gaccaaccac
     2941 ccccagcggg aagtcctatc gtacccaccc gcgtcagcct gcaatattca tcttcttact
     3001 caatggatga tgaaaatacc ccagcccctg agcatgtaac tcaaaaagaa gaaggattta
     3061 aggaacgccg cgccgcccgt gaagaaacca aaaagaaaca attggccgag gctaacacta
     3121 agaaggaaca atgtaatcaa gcccgtagca atatggaaat cttaaagaat acccctcaac
     3181 tgagtatccc ggatggaatg gggggtgtca aagaagctga tgataatttt cgcttaaaac
     3241 aaatggaaga cgtgaaaaaa atgattgcgt cgctatgtta attcgcttca tcaaacctaa
     3301 aaaaaatacc caccagcaca tcgtatttac cgagttaatg atgcagaccc tgggtaattt
     3361 cccagggctt tctagtacgt tcctgaagtt ctgactcgtg tgcctatcag aatttatctt
     3421 ttattcgaat cacatcaaac gataactcag atcgagaaag taagtagctg ttatttcatt
     3481 atagctagtg accttctact tattggtagc caattaactt ctcaactaat gcaaaaggaa
     3541 attagagttg attcattctt ttaaatttat aaaagctgct caagttacat ttacgctgtt
     3601 gttggtgttt atggtgttga tagcaaggaa tagttatggt ttccaaagta gcccgatagt
     3661 atggactaca acggggatat ggcacgccac ccctgaagaa tcatgccatt attgggcatc
     3721 tcgcagagat attgaatatg tgggtattcc tagttccaag tatgctgaga ttaaggctat
     3781 gtgggtaatt ccctgggggt ggaatgaaaa taatgtattg gtaatgaata ttatacctgc
     3841 agttcagtat caatgtgggg cacgtgtttt tcattatgga aatcctccct atcaacaacc
     3901 ttatgagtat gattggcggt ttggtggagc atttccagtt tgtagagtag gtgattcacc
     3961 ttctgttcac agtgatactg tttgcaacat ccccgaccac atcccagata gaggcgcaaa
     4021 cttaggccaa ccctcaacat gtgttggtaa ccccattaat accgctacag gcaataaatt
     4081 cgcccagcaa atcgatgtcg tgctgccaac atttactttt acacgtttct acaattcgtt
     4141 tggattttcg agtaatggtc aaattggtgc ctcctggcgc cataacttcc aaagtagttt
     4201 atttattaac ccaactacga ttacagcaac ccgggcagac ggtaaattaa tctacttccg
     4261 actcgaggga gcggtttgga tcaatcagca gtccagtgga gaggcattag caccaatcac
     4321 tgatgtaagt ggctcagagg gtggctggtt gttaacgact gctgatgatc ataaaaaata
     4381 ctacgacact gcaggcaagc ttgttgcaat ttcagaccgt gcggggcgac gagtttcttt
     4441 aaactatgat acgcattatc aattagctac cgtgactgat gacgtaggtc gaacactgca
     4501 atttacttat gatggtaaca atcgaattaa agcattgatt gacccatcag gagaacagta
     4561 tcagtatcat tatgataatc aaggcaatct gacgcgtgtc acctaccctg ataatcattt
     4621 tattagctat cactacaacg aacccgctta taccagtgga gcaaatttac ctttcgcatt
     4681 aaccggtatt accgatgaaa atggaaatcg gttcgcaatt tatcactatg atgcgcaagg
     4741 aagagcgatt gtaactgaac atacggatgg cgctgatcgg tatcatctgg gctataaccc
     4801 agatggtagc actacgataa cagattcctt aaatacgcaa cgaacacaaa cattccaaac
     4861 cctgtttggg gttgtaaaaa tggtgagtca atcacaacca gcaggctctg gatgtgatgc
     4921 atcatctagc gatataagtt acgatgcaaa tggtaacgtt gctagccgta cggatttcaa
     4981 tggtaataaa acttgtttca gctatgatct cgaccgaaat ctcgaaatag cgcgtgttga
     5041 tggg
//
LOCUS       DAOQLJ010000152         5033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_7696_length_5033_cov_4.958618, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000152 DAOQLJ010000000
VERSION     DAOQLJ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7696_length_5033_cov_4.958618"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..219
                     /locus_tag="PK522_09665"
     tRNA            144..219
                     /locus_tag="PK522_09665"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:177..179,aa:Phe,seq:gaa)
     gene            328..741
                     /locus_tag="PK522_09670"
     CDS             328..741
                     /locus_tag="PK522_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HNC42038.1"
                     /translation="MKNNGSELLPSRRNSKKSIVSLSKQGNDRFPFLHFETDSDKSYI
                     TQMIMEGDSMKPTIEINALLVVDTRVKTYVDDGIYVVSCGIILAIRRIVMSPSRNKFA
                     VSSDNPLIRSVEFVESHELNIVGKVIYSLTGKKIS"
     gene            762..992
                     /locus_tag="PK522_09675"
     CDS             762..992
                     /locus_tag="PK522_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42039.1"
                     /translation="MEKTEYTQPLEQDNPQECFEPFGWKPRIKKHPSEYADFIEDVNF
                     TTRGIETILELLESASLDRGESTTLNRYQKAF"
     gene            complement(1416..1688)
                     /locus_tag="PK522_09680"
     CDS             complement(1416..1688)
                     /locus_tag="PK522_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42040.1"
                     /translation="MTPGQQAAIVASAQDWAKAQVQGANRYTIKTGNVAGLETVADRA
                     SESGASERTQRMADKVAKADPELAKQVAHGEISLPKAVEKISPKKK"
     gene            complement(1826..2110)
                     /locus_tag="PK522_09685"
     CDS             complement(1826..2110)
                     /locus_tag="PK522_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42041.1"
                     /translation="MPTYTPRGINAGIEINESSGLDKEISLIPIMSGYRDKDTFEVSL
                     TTYYEAIDTDLDIVIRQDKILTASWFDFDVYKKLNHKKRQYNTSYIYVND"
     gene            complement(2159..2605)
                     /locus_tag="PK522_09690"
     CDS             complement(2159..2605)
                     /locus_tag="PK522_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein"
                     /protein_id="HNC42042.1"
                     /translation="MKWITRERPKIDRIACPWLIARFIDKESAFLFVPPDQVVAEAKL
                     TGAIPFDIPGVELSHVGNECSFDAFLKKYNLTSPALQDLAAIVRGADTDHLDLAPQCA
                     GLLAISLGLSHNFKDDHEMLAHGMVMYDALYAWCCHARGERHNWDY"
     gene            complement(2617..3972)
                     /gene="chrA"
                     /locus_tag="PK522_09695"
     CDS             complement(2617..3972)
                     /gene="chrA"
                     /locus_tag="PK522_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNC42043.1"
                     /translation="MDVPKQQFPDIADAPKKISLSQAFWYWLKLGFISFGGPAGQIAI
                     MHQDLVERMHWISERRFLHALNYCMMLPGPEAQQLAIYIGWLMHRTWGGIIAGILFIL
                     PSLFILIALSWVYISFSDIALVNGLFYGIKPAVTAIVVQAAYRLGSRTLKNSLLWSIA
                     VASFVAIFVFNLPFPLIVVSAALLGYVGGRIIPDSFKVNSNHSKANKSFGLALIDDHT
                     PTPEHAKFRWLRLVKVVVIGGLLWLLPMILLTISYDWDHTFTQMGWFFTKAALMTFGG
                     AYAVLPYVYQGAVEYYEWLTPTQMIDGLALGEATPGPLIMVVAFVAFIGGYVKALLGP
                     DMLFISGTMAAILVVWFTFLPSFIFILAGGPLVESTHDNIKFTAPLTAITVAVVGVIL
                     NLALFFGYHVMWPQGFSGNFDWISALIALLAGIALFQYKRNVIEVIAICAIIGLILNL
                     T"
     gene            complement(4212..4508)
                     /locus_tag="PK522_09700"
     CDS             complement(4212..4508)
                     /locus_tag="PK522_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42044.1"
                     /translation="MLSGVKADPSANLQQGETGRTCEELSELSGVSSRNIYKVKNIIS
                     TGSDELIETVRAGEISINVAEDIATLPQEGQKEIDLTDTSLNRVIVRSGRDYYN"
     gene            complement(4619..4819)
                     /locus_tag="PK522_09705"
     CDS             complement(4619..4819)
                     /locus_tag="PK522_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42045.1"
                     /translation="MFMACHSQEEIAEVAGADQATIKRKVDDFMQNSNLAKMHKTLAN
                     HEDSEFQIPLYNVWTKSKITTA"
BASE COUNT         1538 a         1027 c          953 g         1515 t
ORIGIN      
        1 agtacttggc tacgaaccaa ggggtcgtgg gttcgaatcc tgccgggcgc gccatagatt
       61 agtcagtata attgtaggcg ttagtaaata ctatttttgt tttgggaact tatttctgaa
      121 agcaaaaata aataatccaa ataggccaag tagctcagtc ggtagagcag aggactgaaa
      181 atccttgtgt cggtggttcg attccgccct tggccaccaa taaaatcaat aacttacgat
      241 tattgatttc tagctaatat cgttcatgtt tttccgtgta gtcttcctgt aggaaatttt
      301 caagaatcta aacggagaaa acacagcatg aaaaataatg gcagcgaatt acttccttca
      361 cgaagaaact cgaaaaaatc gattgtttct ttatctaagc agggaaacga tagattccct
      421 ttccttcatt ttgaaacaga ttcagataaa agctacatca cccagatgat tatggaaggt
      481 gattccatga agcccacaat tgaaataaat gcgcttcttg tcgttgacac tagagtcaaa
      541 acttatgttg atgacggaat ctatgttgtt tcctgtggga tcattttagc tattagaaga
      601 atagtgatgt ccccaagtag gaacaaattt gcagtgtcat ctgataaccc gctaatacgg
      661 tcggttgaat ttgtagaatc acatgagcta aatatcgtag gcaaggtcat ttactcgcta
      721 acaggtaaaa agatttctta acgttattat aaggataagc catggaaaag actgaataca
      781 ctcaaccatt agaacaagat aatccgcaag aatgttttga accttttggt tggaaacctc
      841 gtataaaaaa acacccaagt gaatatgctg attttattga agatgttaat tttactacta
      901 gaggtataga gacaattctt gagttattag aaagtgcttc acttgatcgt ggcgaaagca
      961 caacactaaa taggtatcag aaggcgtttt aattcgcttt gctataacgt caagcaaaat
     1021 gcttaccgat agagcttgtg aacttttatt agatattgat gaaaagcgca tcaagcaagc
     1081 cagtaagaat ctaagaatta gcaaataata agatttgcta ctgctatagc tgctattttt
     1141 gctacataca gataaaccag tgatgatttt taaagataca ttagaaaaat tttattttta
     1201 ctttactaat taatcactta gttatcatta tactttgatt gcttggtaat ccccaagtgt
     1261 cgttttttaa tgtagtagca gtttattcaa gaggaaagta tatacctcca tttatgcttt
     1321 cctacttttt caagcataac tcacttgtag cgattgcgta gccccatctt ggccttgaca
     1381 acgctatcag cttttatctt catcatcctc aattactact tcttctttgg agatattttc
     1441 tcaactgctt ttggtagaga tatctcaccg tgtgccacct gtttagctaa ctcaggatcg
     1501 gccttagcta ctttgtctgc catgcgttga gtgcgttcgc ttgcaccaga ttcgctagct
     1561 ctatctgcta cagtttctag accggcaacg ttaccggtct taattgtgta tctatttgct
     1621 ccttgcactt gcgccttagc ccaatcctgt gcactagcaa caatcgcagc ctgttggcct
     1681 ggcgtcatgt gtcttctgtg caagtttgca gacaacacaa atgaaacaat attgtcacca
     1741 tcaaaatcaa caaactcagg ttctatgcca gcctcaacgc atgctctata atacaaatta
     1801 tgtatagcag ctcaaaagtt aacttttaat catttacata gatgtaagaa gtattatatt
     1861 gacgtttttt atgatttagt tttttatata catcaaaatc aaaccaactt gccgtaagta
     1921 ttttatcttg gcgaatcaca atatctagat ctgtgtctat tgcttcataa taagtagtaa
     1981 ggctcacttc aaatgtatct ttatctcgat atccactcat tattggaatt aatgaaattt
     2041 ccttatctaa tccgctactt tcatttattt ctattccggc attgattcca cgcggtgtat
     2101 atgtgggcat tgaaaaacgt ttctgaaaaa gcaattaacc aaaaattggt gaattccttt
     2161 aataatccca gttatgccgt tctcctcgag catggcaaca ccaagcataa agtgcatcat
     2221 acattaccat tccgtgtgca agcatttcat gatcatcttt aaagttatgt gataaaccta
     2281 acgatattgc caacaaacct gcgcactgtg gcgctaaatc aaggtgatca gtatctgcac
     2341 cgcgtactat cgcagctaaa tcttggagcg ctggactggt caaattatac tttttgagaa
     2401 acgcatcaaa gctacattca ttaccgacat gagataattc tacgcctggg atatcgaatg
     2461 gaattgcgcc agtcaatttt gcttcagcta cgacctggtc aggtggcaca aataaaaacg
     2521 ctgattcttt atcgataaat cgagcaatta accaaggaca ggcaatacgg tcaattttgg
     2581 ggcgttcacg agtaatccat ttcatggttt ctttccttat gtgagattta gaatcagtcc
     2641 gatgattgcg cagattgcga tgacttcaat cacattgcgt ttatattgaa acaaagctat
     2701 acctgccaac aaagcaatta gggctgatat ccaatcaaaa ttaccggaaa atccctgtgg
     2761 ccacattacg tgataaccga aaaacaaagc caaattgaga ataacgccta caacggccac
     2821 cgttatagca gttaatggtg cggtaaattt aatattgtcg tgtgttgatt ctaccagagg
     2881 accaccggca agaataaaga taaaagaagg tagaaatgta aaccaaacga ctaagattgc
     2941 agccattgtt cccgaaataa ataacatatc tggtccaagg agggctttca cataaccacc
     3001 aataaatgcg acgaaggcca ctaccatgat aagcggacct ggtgttgctt cacccagtgc
     3061 taacccgtca atcatttgcg ttggtgtaag ccattcataa tattcaacgg caccttggta
     3121 aacatacggc aataccgcgt aagctcctcc aaaagtcatc aaagccgcct tagtaaaaaa
     3181 ccagcccatt tgagtaaatg tgtgatccca atcgtagctt atcgttaaca atatcattgg
     3241 cagcagccac agaagtcccc cgattacaac taccttaact aatcgaagcc aacgaaattt
     3301 tgcatgttct ggcgttggtg tatgatcgtc aattaaagct aaaccaaagg atttgtttgc
     3361 tttactgtga ttactattta ctttgaagct gtctggaata atgcgtccac ccacatagcc
     3421 aagcaatgca gcgctaacaa caatgagtgg aaatggtaga ttaaatacaa agattgcaac
     3481 aaatgaagca actgcaattg accatagcaa actatttttt agcgttcgtg aacctagtcg
     3541 atatgcagct tgcactacga tggcagtaac cgcaggcttt ataccataga ataaaccatt
     3601 tactagagca atatcgctaa agctaatata aacccatgaa agtgcaatta aaataaatag
     3661 cgacggtagt atgaataata tcccggcaat gattccaccc catgttcgat gcatcaacca
     3721 gcctatatat atggctagtt gttgagcttc gggacctggc aacatcatgc aatagttgag
     3781 ggcgtgcaga aaacgtcgtt ctgaaatcca gtgcatacgc tcgaccaagt cttggtgcat
     3841 gatagcaatc tgacctgctg gtccaccgaa actgataaaa ccaagcttta gccaatacca
     3901 gaaagcttgt gataaggaaa tttttttagg ggcatctgcg atatcaggaa attgctgctt
     3961 tggtacgtcc attactcctc cttggtatta tccaatttta gagtagtcgt tggacgatga
     4021 tcgtcttgca gtacgcgatg gggagactac tttatccccg atttcgctat tgtgtatgta
     4081 tcaaaagtga aaagcaatag cgaactgtaa aagatattat cacaggtgaa acagcactaa
     4141 taaaaatcac aactgattga tgtatttaac cgacgtggat tcaacttcga agtgcaacac
     4201 ttaacggtga attagttgta gtagtctcga cctgatctga caattacccg atttaacgat
     4261 gtgtctgtta gatcaatttc tttctgccct tcttgcggta gcgttgcgat atcttcagca
     4321 acatttatag atatctcgcc agcgcgaacc gtttcgataa gctcatccga tccagttgaa
     4381 ataatgttct ttactttata gatgtttcta gaagagacgc ctgataattc tgacaattct
     4441 tcgcaagttc tgcctgtttc accctgttgc aaatttgcac tagggtctgc tttgacccca
     4501 gataacattc tttctttcgc cttttcagca atcagcggtt cggcctgaag cgctatttca
     4561 gcacgctgaa agtcggttaa attacgacgg ccaagttggt tcttaagaat ccaaagcttt
     4621 atgctgtagt gatctttgac ttggtccaga cgttgtaaag cggtatttga aattctgaat
     4681 cttcgtggtt ggcgagagtt ttatgcattt ttgcaaggtt gctgttttgc ataaaatcat
     4741 caacctttct ttttatagtt gcctgatcag caccagcaac ctcagcaatt tcctcttgcg
     4801 aatgacacgc catgaacatg tcaaaaataa tctgattcgt tttctcgcgc tcatcgcaat
     4861 atagcaagaa tagcagctat agcagtagca aatcttattt cttaaaaatc taacatttaa
     4921 acagccacgg tttttcgtca gtagattgat tagctaatgc acgaaatgca gtcgttgggt
     4981 gaactgcttg ctttgtaata cgaagacgtg tcgcaaaaga agaggccatt tta
//
LOCUS       DAOQLJ010000153         5030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_7707_length_5030_cov_4.696683, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000153 DAOQLJ010000000
VERSION     DAOQLJ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7707_length_5030_cov_4.696683"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..2868
                     /gene="prsT"
                     /locus_tag="PK522_09710"
     CDS             79..2868
                     /gene="prsT"
                     /locus_tag="PK522_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HNC42046.1"
                     /translation="MWPKNKAGFFAVVAITWGASVTTLPGCSGKKSSAEYLVAAQGHE
                     KKGEYDAALIELKNLLAADSGNAAGRLMFAKVLNRKGDGVSAERELLKIAKHAQGTAA
                     YRIELGRALLLQNNADRIIKEITPAGLQMDDATTILSIRGGAYLSLRQLDNARAAFAE
                     ALKINPKHVQSMLGLASIAIATNDMRSAESNVDSALAIAPRDVDALLFKGDLLRQQGK
                     PQEALASYMDAAKVDPTQYSPLLRLAVLQIAQGKLTEAKESLAKVEKIHSGLSDRRYF
                     SALIYFREGQIEQAQSQVQDVLKQAPNNAPANLLAGAIAYRKGYYSSAEAYLEPVVKV
                     FPMHTPTRKLLADVQLKAGHTERALATLQTGLKLLPDDEFLLLQAGDASYQAQHFAQA
                     NEYYAKASKLSPDNLTLRTRHGMSQLALGQTEQAMAELEFIANEDKIGGQADYLLVRS
                     YLNRKDYGKAMNTLSMLQKKQPKNPQVYHLKADVLLAQNNRQAAIQALEEAYSLRPSY
                     LPTVMRLVSFDIQNEKPEVAQKRFEYLLETDPKNVDAMTAFAVFLFQQVGKREQAMVL
                     LERAHKLQPSAIAPATVLIEQLIFAGEKNKALSYAQQIVADRPDSSEAIYLLARTQLT
                     NGQNNQALASYHKLVSLTPDAPMSHFRLAGVQASMNDNEGAQSSLKKVLQLQPDFVDA
                     LVLLVKLQVKVGNLSGAFETVKAAQQRPTSAYQAYILEGDLLVEKKQPDDALASYQKA
                     LELNNNAVPLIKICALHKLVTNQKKIELFVAQWLKANPADLTVRTFLAQSNLRERKYL
                     QAAEYYREIIKLKPNNTDALNNLAWVYHRLNDPRALNIAEAAVAQNPGNPLAMDTLAV
                     ILLQKGEKRRSLGLLQKAATSRPADAEISTHLAWALSVNGLHDQAKAVLLRVEASGDS
                     FVKQAEFKEIASLIR"
     gene            complement(2910..3710)
                     /locus_tag="PK522_09715"
     CDS             complement(2910..3710)
                     /locus_tag="PK522_09715"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC42047.1"
                     /translation="MNIQDFRGQWALVTGASSGIGAEFCKQLASLEVNLVLVARRDDN
                     MQLLAAQLQSEHHIRTLVLPYDLAESGCPRALKESVQSKNIKIRLLVNNAAYGPWGSF
                     GKTSAESYEKLCQLVMGTPIALCREFLDDLSSFSGAAIINLSSPAALQPVPYKAAYSA
                     AKTGLHFFSLALYEELKYRNIYVQTLMPGPTQSELDEKGNAYKSALAEERRLPSEIVN
                     ASLAAFTKKQPFVTTAKGTYKQRLFTGLFPIKFVLKTVAQMFKPPENR"
     gene            complement(3784..4818)
                     /locus_tag="PK522_09720"
     CDS             complement(3784..4818)
                     /locus_tag="PK522_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNC42048.1"
                     /translation="MISFSRRNFMKKSILAAATLAALGCASTIYASTSVKIDLDGSGA
                     GSTDSQYNVTSLDWDVGNSLSVAKQNESLAAGSTYVGQVFATYFHANLGNFKRIVGGI
                     DKVIGGTNLNNTYEWTVVAGFAEKFSEVTPSPGLGSLAFDVLNQSSNFFQIWYDTSMD
                     YNNLTGRGFNNGKLILEGRIISGQGKFVAEGGLDANGQPNHPLDTYNSDDYPNIKTMT
                     GQGSTSVTVQVTNQDSDFFKEHLDSFVLDFTTQIKLPYITQDPSACFWNGLAEIGGAG
                     GQGTGCANTIGTLNGGPALMGGGPNLALQVDASNNFNSNKVPEPASLAVLGLGALAMC
                     AASRKNRNAA"
BASE COUNT         1314 a         1115 c         1221 g         1380 t
ORIGIN      
        1 cgatcagtcg ggtattgccg ctaccgcccg caaattgctg ggcagccttt aacatctatt
       61 aagccgaaag agtgaattat gtggcctaag aataaagcag gatttttcgc tgtcgtagca
      121 attacttggg gagcaagcgt aaccactttg ccggggtgct caggcaagaa atctagtgct
      181 gaatatctcg tagcggcgca aggccatgag aaaaaaggtg aatatgatgc agcgttgatc
      241 gagctgaaga acttgctggc agcggattct gggaatgccg ctgggcgact gatgttcgct
      301 aaggtactta atagaaaggg agatggcgtt tcggctgagc gtgaattact caaaattgca
      361 aagcatgcac agggaacggc agcctatcgt attgaactgg ggcgcgcatt attattacag
      421 aataatgcag ataggattat caaggagatc actccagctg gccttcagat ggatgatgcc
      481 acgacaattt tgtccattcg tggtggcgcc tatttgtcgt tgcgccagct tgataacgca
      541 cgagctgcgt ttgctgaagc gctcaaaatt aacccaaaac atgttcaatc aatgcttggt
      601 ttggcaagca ttgcaattgc gactaacgat atgcgcagcg cagaatcgaa tgtcgacagc
      661 gcgctggcta ttgctcctcg tgatgtggat gccttgctgt tcaaggggga ccttttgcgg
      721 cagcaaggta agccccaaga ggcattggct tcatatatgg atgcagccaa ggttgatcca
      781 acgcaatact ccccattact tagactggcg gtattgcaga ttgcccaagg taagctgact
      841 gaagcaaaag agtctctggc caaagtggaa aaaattcact caggtcttag cgatagacga
      901 tatttctcgg cgctgattta tttcagggaa ggccagattg agcaggccca gtcgcaagtg
      961 caggatgtgt tgaagcaagc tcctaataat gcaccggcaa atttgctggc cggtgccatt
     1021 gcgtatcgga aggggtacta ctcctcggca gaggcttacc ttgagccagt tgtcaaggtg
     1081 tttcctatgc atacacccac ccgtaagtta ctggcagatg tccagctaaa ggcaggccat
     1141 accgagcgtg cgctggcaac gctacagacc ggcttaaaat tgcttcctga tgatgaattt
     1201 ttgctattgc aggctggcga tgcaagctat caggcgcagc attttgcgca ggcgaatgaa
     1261 tattatgcca aggccagcaa gctctcaccg gataacctca cgttacgtac ccgtcatgga
     1321 atgagtcaac tggcgcttgg gcaaaccgag caggcgatgg ctgaactcga gtttattgca
     1381 aatgaggata agattggagg ccaggccgat tacctgttag tacgttccta tttgaatcgt
     1441 aaagattatg gcaaggcgat gaataccctc tccatgttgc agaaaaagca gccaaagaac
     1501 cctcaggttt atcacctcaa ggctgatgtt ttgctcgcac agaataatcg tcaggccgct
     1561 atccaggcat tagaagaggc ttactccctc cgtccctctt atttgccaac ggtgatgcga
     1621 ttggtgtcct ttgatattca aaacgaaaag cccgaggtgg cgcaaaaacg ttttgagtac
     1681 ttgctggaga ccgaccccaa aaatgtagat gccatgactg cgtttgcggt ttttcttttt
     1741 cagcaggtgg gtaagcgcga gcaagcaatg gttttgttgg agagggcgca taagcttcaa
     1801 cctagcgcaa ttgctcctgc gacggtgctg atcgaacagt taatttttgc tggcgaaaag
     1861 aacaaggctc tctcttatgc ccagcaaata gttgctgacc gaccagatag ctccgaggct
     1921 atttatttat tggcgcgaac gcagctgacg aatgggcaga ataaccaagc tttagccagt
     1981 tatcataagc tcgtttcatt gacacctgat gcgccaatga gtcacttccg acttgctggt
     2041 gttcaggcaa gtatgaatga caatgagggg gcccaatcta gcctaaagaa agtgctccaa
     2101 cttcagccag attttgtaga tgcattggta ttgttggtga agttgcaggt taaggttggc
     2161 aacctatccg gagcgtttga gacggtaaaa gctgctcagc aacgtcctac tagtgcttat
     2221 caggcatata ttctggaagg cgatttactt gtggagaaga agcagcctga tgacgcattg
     2281 gcctcctatc agaaggcgct tgaactcaat aataatgcag tgccattaat aaagatatgt
     2341 gctctccata agcttgttac aaatcagaag aaaattgagt tatttgttgc ccaatggcta
     2401 aaagctaatc cagccgattt gactgtgcgg acttttcttg ctcaaagtaa tttacgggaa
     2461 cgcaagtatc ttcaagccgc cgagtattat cgggaaatca ttaaactaaa acctaataac
     2521 acggacgctt taaataatct agcatgggta tatcatcgtt tgaacgatcc tcgtgctctt
     2581 aatatagccg aagctgccgt cgcgcagaat cccggcaacc cccttgcgat ggatactttg
     2641 gcggttattc tgctacagaa aggagaaaag cgtcggagcc ttgggctact tcaaaaggcg
     2701 gctacctcgc gcccagcaga tgcagaaata tcgactcatt tggcttgggc gctgtctgtt
     2761 aacggcctgc atgaccaagc aaaagcagtt cttcttaggg tggaggcgtc gggagattct
     2821 tttgtaaaac aagcagaatt taaggagatt gctagtctaa taagatgact gctagctatt
     2881 gagtcgatta tcctttagat cgatgtttgt tatctgttct ctggtggttt aaacatttgt
     2941 gccactgttt ttaatacaaa ttttatgggg aataaccctg taaataatct ttgcttataa
     3001 gtgcccttgg ctgttgttac aaaaggttgt ttcttggtaa aagcagctag cgaggcattg
     3061 acaatttcag agggtagacg tctttcttct gctagagcgc ttttataggc attgcctttt
     3121 tcatctagtt cgctctgtgt gggtcctggc ataagtgttt gtacatagat gtttcggtat
     3181 ttcaattcct cgtataatgc taggctgaaa aaatggaggc ctgtttttgc cgccgaatat
     3241 gctgccttgt atggaactgg ctgcaaggct gcgggagatg ataggttgat tattgccgca
     3301 cctgagaaac tcgaaagatc gtcaaggaat tctctacaca atgcgattgg agtgcccatt
     3361 accaattggc agagcttctc atagctctcc gcagatgttt ttccaaagct tccccaaggc
     3421 ccatatgccg cattattcac aagtaagcgg atcttaatgt ttttagattg gacagactct
     3481 ttgagcgctc gtgggcaacc agattcagca agatcataag gtagaactag tgttctgatg
     3541 tgatgttcgc tttgtagctg ggccgccaag agttgcatgt tgtcatctct acgcgccacc
     3601 aaaacaagat ttacttctaa acttgccagt tgtttgcaga actcggcgcc aatcccagaa
     3661 gatgctcccg ttacgagtgc ccattggcct ctaaaatctt ggatgttcat cgttgcgtcc
     3721 aaataataaa ggggacatct cccctttatt atcaataaaa caggctttta attgcggcca
     3781 aatttaggct gcatttcgat ttttacggct tgcagcacac atcgctaacg cacccagacc
     3841 aagtactgct agtgatgctg gctcgggtac tttattgcta ttaaagttgt tgctggcatc
     3901 aacttgaagt gccaagttcg ggcctcctcc cataagtgcc ggcccgccat tgagtgtacc
     3961 aatcgtgtta gcgcaaccag ttccttggcc acccgcccct ccaatttctg ccaagccatt
     4021 ccagaagcat gctgacggat cttgagtaat atagggtaat tttatttggg tagtaaaatc
     4081 aagaacgaaa gaatccaagt gctccttgaa gaaatcagaa tcttgatttg taacttgtac
     4141 ggttaccgac gtcgagcctt ggcctgtcat tgttttgatg ttcggatagt cgtcactgtt
     4201 gtaagtgtcc agcggatgat tcggctgacc attggcgtcc aagcctcctt ccgcgacaaa
     4261 cttgccttga cccgaaataa tccgaccttc cagaatcagc ttcccattat tgaagcctcg
     4321 accggtcaag ttgttgtagt ccatgcttgt gtcataccaa atctggaaaa agttgctgct
     4381 ttgattcaat acgtcaaacg ctaacgatcc caagcctggg cttggcgtaa cttcggaaaa
     4441 cttctcggca aagccagcta caaccgtcca ttcataggtg ttgttgagat ttgttcctcc
     4501 gataactttg tcaatcccac caacaatgcg cttgaagttg cctaggtttg catggaaata
     4561 tgtagcaaaa acttggccga cataagttga acctgctgcc aaggattcgt tctgtttagc
     4621 tacggacagc gaattgccta cgtcccaatc tagcgaagtc acgttgtatt ggctatcagt
     4681 gctacctgca ccacttccat ctaagtcaat cttcacggat gtgctcgcat aaatagtgct
     4741 agcacatcca agggcagcaa gcgttgctgc tgcaagtatc gattttttca tgaaatttct
     4801 cctggaaaat gagatcacgg aactaagtta gctcggtgct atatgtcaag cacgatgtgt
     4861 gccagttttt gtttttgatg taagttattg aaaagtagtg tctatttttt ttttataaag
     4921 tgattatgtt ttcggtgtaa aaaaaatcga caaaatagga aagcgtctgt acggattgct
     4981 ctagggttag tagatgcatt tcccgatacc tattggctgg gctccatgaa
//
LOCUS       DAOQLJ010000154         5012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_7752_length_5012_cov_5.125681, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000154 DAOQLJ010000000
VERSION     DAOQLJ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7752_length_5012_cov_5.125681"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PK522_09725"
     CDS             complement(<1..605)
                     /locus_tag="PK522_09725"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HNC42049.1"
                     /translation="MKNNIEIIALAEEGSSLLAQTKSVILSAESDEIKSLATRMPDIL
                     EGQSGPLKLVFAGQYSAGKSSVISALTGRNDIAIGAGITTQHTHSYHWKGVDIIDTPG
                     VHTSLRPDHDEITYRALADADLLVFVTTNELFDDHLAKHFRKLAIDRDKGREMLLLIN
                     KMQRHVSGNSPEAWQIITEELQKVVTPFTPEDLHISFVDAES"
     gene            complement(626..2734)
                     /locus_tag="PK522_09730"
     CDS             complement(626..2734)
                     /locus_tag="PK522_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HNC42050.1"
                     /translation="MSNNSLNKIAPNNVELEHALKVCHGFAEKGYQVAHDNLKRTGDA
                     IQHVELTIQNTLQSIEKSQVHSPDILDSIQQQLNKVMGDLHILQRDSEAKLEERRKQL
                     KSFNISLFGRTMAGKSTLMEILTNGDGESIGKGKQRTTRSIRTYTWKGLTVTDVPGIA
                     AFEGHVDEVVAFRVATMADLVLFLITDDSPQPAEADCFVKLKQLGKPILAVCNVKVAI
                     KDPEDLMDFLSDPDYAFNVVRLNELTQQFIALVDQHLPGASAPFIAIHAHARYLADKP
                     EYSQLKKKLLEASRFVTFEKQLIKHVIDNGRFFRLKTFIDASVVPMFKLSEELLIFKE
                     KNSSSNRVLTKKKKQFVAWKNAFLNRGCEKIDAFCGEMGGILSAEIASFSEDNYENKN
                     AGDAWDRVVKSLNIEKKSSDLMNNLHKEAETFLMEFFREVNAELTLVDSLKAESIVMP
                     SITDTRKIARWLGVLATGAITLAALLGPVSWMVVAGVAVVSAFFSWFTDSKDSKAAKA
                     RSKLESILEKHVDKLKKHYHDSLSKWFKHELIKKQIELVESNLDIIISALSSLANTQV
                     ELAEILTNRQAEMNYELVKESLQDAHKDNFIGGLLRVARAPGYMVVLCFRMGISPPVI
                     YREKLELLLAETVIFIVGDYTDLEILAHLFKQKLGEQVHNIVIDSHLKVARVPLKALD
                     EEACYLAAIAYQLTELHVIV"
     gene            complement(2727..>5012)
                     /locus_tag="PK522_09735"
     CDS             complement(2727..>5012)
                     /locus_tag="PK522_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNC42051.1"
                     /translation="GVAETLGEYTVKKQLEIPENLDELDEELSASEYEMYAEQVVDQA
                     TAAETIYELDAEIVILKDLEYQALQVVQSKSDRKWEELSSLLQDKPEMFTASGSRRKL
                     IIFTEHKDTLNYLVGRISDMLGRPEAVVIIHGGTNRDQRRKAQEEFRNNPDVVVLIAT
                     DAAGEGVNLQNANLMVNYDLPWNPNRLEQRFGRIHRIGQTEVCHLWNLVANETREGEV
                     FKKLFDKLEIEKKALGGKVFDILGEAFENQSLRDLLIEAIRYGESAEVKKRLNEVIEG
                     ALDTRHLKEILRRNALVEQHMSLEDLYAVKEEMEKAEARKLQPYFIRAFFCEAFSLLG
                     GEMRPRESGRFEVRHVPALFRERDRVIGETRTPVLKKYERICFDKQQVRMDGKPMADL
                     IHPGHPLMHTTTDLILTLHRAKLKQGAILVDPNDDSTEPKILFMIDHSVRQSITDNPV
                     VVSRRLQFVEIDSQSKVSNSGWAPHLDLEAINEYDLSLVKNILEHSWLKNDLDSLALQ
                     YASRHLVPDHYQEVKAKLEKQADKTLAAVNERLIKEINYWSDRYIKLTDDVAAGKQPR
                     VQPEMARRRVDELTERLNQRKRELAAMKNVVSSTPVILGGALVIPQGLLAQRKGETAF
                     CVDAHARARVERIAMEAVIAGEQAKGFIVQDVSAQKCGWDITSRPPVNEDGSILPDRH
                     IEVKGRSKGQSTITVSRNEILYGLNQADKFILAIVLVNLDDTFEGPYYIRNPFTKEPD
                     WAETSKNLDLVALLERASKDV"
BASE COUNT         1456 a         1136 c          827 g         1593 t
ORIGIN      
        1 gactcagcat caacaaaaga aatatgcaaa tcttcaggag taaagggtgt cactactttt
       61 tgcaattcct ctgtaataat ctgccaagcc tcaggcgaat ttcctgaaac atgcctttgc
      121 attttattaa tcagtagtaa catttctcgg cctttgtctc tatcaattgc cagcttacga
      181 aagtgttttg caaggtgatc atcaaataac tcatttgtgg taacaaatac caataaatct
      241 gcatcagcta atgctcgata tgtaatttca tcatgatcag gtctaaggga ggtatgaaca
      301 cctggtgtgt cgataatatc aacacctttc cagtgataag agtgtgtatg ctgcgtcgta
      361 atacctgcac caatagcaat atcgtttcgc cctgttaatg cactaatgac acttgattta
      421 cctgcactgt actgccctgc aaatactagc tttagtggac cactctggcc ttcaagaatg
      481 tctggcattc tagtagcaag acttttaatt tcatcagact ctgcgctgag aataacactc
      541 tttgtttgtg caagaagcga agacccctct tctgcaagcg caataatttc aatattattt
      601 ttcattttat gatccttttg attgtttaaa caatgacatg gagttctgtt aattgatacg
      661 ctattgcagc aagataacaa gcttcttcat ctaatgcttt taatggcacg cgggcaacct
      721 taaggtggct atcaattaca atattgtgca cttgctctcc cagcttttgt ttaaacagat
      781 gcgccaatat ttccaaatcc gtataatcac ctacaataaa aataactgtc tcagccaaca
      841 atagttctag cttttcacga tagatgactg gagggctaat acccattcga aagcaaagga
      901 cgaccatata acctggcgcg cgggcaaccc gcagcaatcc accaatgaag ttgtccttat
      961 gagcgtcttg tagtgactct ttaaccaact cataattcat ctctgcctga cgattggtga
     1021 gaatttctgc aagttcaact tgtgtattgg caaggctcga taacgccgaa ataatgatat
     1081 caagatttga ttcaactaat tcaatctgtt ttttaattag ctcatgttta aaccatttag
     1141 acaaactatc gtgataatgc tttttaagtt tatcaacatg cttttcaagt attgattcga
     1201 gtttagagcg tgctttcgca gccttagagt ctttagaatc agtgaaccac gaaaaaaatg
     1261 cactgactac tgccactcca gcaactacca tccaacttac aggccccaac aaagcagcca
     1321 aagtaatagc tcctgttgct aaaactccta accatcgcgc tattttgcgc gtatcggtaa
     1381 tactgggcat tacaatggat tcagccttga gactatctac cagtgttaac tcagcgttca
     1441 cctctctaaa aaactccatc agaaaagttt ctgcctcttt atgtaggtta ttcatcaaat
     1501 ctgaagactt cttttcaatg ttcaaacttt tgactactct atcccaagca tctccagcat
     1561 ttttattttc ataattatct tctgaaaaac ttgcaatttc agcacttagt atccccccca
     1621 tctccccgca gaatgcatca attttttcac aacctctatt taagaacgca ttcttccacg
     1681 caacaaactg ctttttcttt tttgtaagta cgcggttgga cgaagagttt ttctctttaa
     1741 atatcaatag ttcttctgac aatttaaaca ttgggacaac tgaagcatct atgaaagttt
     1801 ttagacgaaa aaaccttcca ttatcaatca catgtttaat taattgtttt tcaaaagtaa
     1861 caaatcgact agcctccaat aatttctttt tgagttggct atattctggt ttatctgcca
     1921 agtagcgggc atgagcgtga atagctatga aaggcgcaga tgcgcctggt aagtgctgat
     1981 ctacaagagc aataaattgc tgcgtcaatt cgtttaatct tactacatta aacgcataat
     2041 caggatcgct taaaaaatcc attaaatctt caggatcttt aatcgcaact ttcacattgc
     2101 atactgcgag aattggctta cctaactgtt taagctttac aaagcaatca gcctcagcag
     2161 gttgtgggct atcatcagta atcaagaaca gtacaagatc tgccatagtt gctactctaa
     2221 atgcaacaac ctcgtccaca tgaccttcaa aagcagcaat acctggcaca tcggttactg
     2281 ttaatccctt ccaagtatat gtacgtatgc tcctagtggt tctctgctta cccttaccaa
     2341 tactttcgcc atcgccattc gtaagaattt ccattaaggt cgactttcca gccatggtgc
     2401 gcccaaaaag actgatatta aatgacttta attgttttct acgttcttca agcttagctt
     2461 cagagtcacg ctgtagtatg tgcaaatctc ccattacttt attgagttgc tgctgaatac
     2521 tatcaagaat atcaggtgag tgaacttggc ttttttcaat tgactgtaag gtattttgaa
     2581 ttgttaactc aacatgttga atagcatcgc cagtacgttt gagattatca tgagcaacct
     2641 gatagccctt ttccgcaaag ccatgacaga ctttcagtgc atgctcaagc tcgacattat
     2701 tgggtgcaat tttatttaag ctgttattag acatctttgc ttgccctttc taataatgct
     2761 actaaatcta aatttttact ggtttctgcc caatcgggct ctttagtaaa ggggttacga
     2821 atgtaataag gaccttcaaa agtatcgtcc agatttacta aaacaattgc caatatgaat
     2881 ttatctgcct gattaagacc atatagaatt tcgtttcggc ttactgttat cgtactttgc
     2941 cccttagaac gtcctttcac ctcaatatgt cgatctggta aaatagaacc atcctcattg
     3001 actggtggac gagacgttat atcccaacca cacttttgag ccgatacatc ctgtactata
     3061 aaacctttgg cttgttcgcc agcaattact gcctccattg caattctttc tactctcgcc
     3121 cgcgcatgag catctacaca aaatgctgtt tcaccttttc tttgggctaa taatccctgt
     3181 ggaatgacca atgcaccacc taatatcaca ggcgtactgg aaactacatt tttcatcgca
     3241 gccaactctc gcttacgctg attaagtctt tccgtaagtt catcaacgcg ccgacgtgcc
     3301 atttctggct gaactcgtgg ttgtttgccc gcagcaacat catccgtcaa tttgatgtaa
     3361 cggtctgacc agtaattaat ttctttaatg agtctttcat tcacagctgc tagcgtttta
     3421 tcagcttgct tttctaattt tgctttgact tcttgataat ggtctggcac taaatgacga
     3481 gaagcgtatt gtaatgctaa agaatctaaa tcatttttaa gccaagaatg ctccagtata
     3541 ttttttacta agctgaggtc gtattcatta attgcttcaa gatctaaatg tggagcccat
     3601 cctgagtttg aaactttaga ttgactgtca atttctacaa actgtaaccg acgagacaca
     3661 acgacaggat tatcagtgat actttgacgt actgagtgat caatcataaa gagaatcttt
     3721 ggttcagtag agtcatcatt gggatcaact aatattgcac cttgttttaa cttagcacga
     3781 tgcaaggtca aaataagatc agtagtagta tgcatcagtg gatggcctgg atgaattaaa
     3841 tcagccattg gcttaccatc catacgaact tgctgcttat caaaacaaat acgttcgtat
     3901 ttctttaaca caggtgtacg ggtttctcca atgacacgat ctcgttcgcg aaagagtgct
     3961 ggcacatgtc ggacttcaaa acgacccgac tcacgaggac gcatttctcc accaagtaac
     4021 gaaaatgctt cacagaaaaa agcgcgaata aaatatggtt gtagtttcct tgcttcggct
     4081 ttttccattt cttcctttac agcatataaa tcttctaaag acatatgctg ttcaaccagc
     4141 gcattacgac gaagaatttc ctttaaatgc ctagtatcca atgccccttc aataacctca
     4201 ttcagacgct ttttaacctc tgctgactcg ccgtaacgaa ttgcttcaat caataaatct
     4261 ctaagtgatt gattctcaaa ggcctcaccc aaaatatcaa atactttacc acctaatgct
     4321 ttcttttcta tttccaactt atcgaataat tttttgaaaa cttcaccttc ccgagtttca
     4381 ttagcgacca aattccacaa atgacaaacc tctgtttgtc cgatacgatg tatacgacca
     4441 aacctctgct ctaaacgatt aggattccaa ggtaaatcat agttgaccat taaattggca
     4501 ttttgtaggt taacaccttc gcctgcagca tcagttgcaa tcaatacaac tacatctggg
     4561 ttattcctaa attcttcctg tgctttacga cgttgatctc gattagtacc accatgaata
     4621 atgacaacag cttctggcct acccaacata tcagatattc gacccaccaa gtaattcaag
     4681 gtatctttgt gttctgtgaa tataatcaac ttacgacgac taccactggc ggtaaacatt
     4741 tcaggcttat cctgcaaaag actagataac tcctcccatt tacgatcact tttagactga
     4801 acaacttgta gtgcctgata ctctaaatct ttcaaaataa caatttcagc atctaactca
     4861 taaatcgttt cagcagctgt agcttgatca accacttgct cggcgtacat ttcatattct
     4921 gatgcgctta actcttcatc aagttcatct aaattttcag gaatttcaag ttgctttttc
     4981 acagtgtatt cgccaagcgt ttcagcaact cc
//
LOCUS       DAOQLJ010000155         5004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_7770_length_5004_cov_5.600727, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000155 DAOQLJ010000000
VERSION     DAOQLJ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7770_length_5004_cov_5.600727"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..544
                     /locus_tag="PK522_09740"
     CDS             80..544
                     /locus_tag="PK522_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNC42052.1"
                     /translation="MSELAHANIAQEFLTFRLGNEEYGIEILKVQEIRGYDTITQIAN
                     APEFIKGVVNLRGIIVPIIDMRIKFNLGEATYDRFTVVIILNLSGRVVGIVVDGVSDV
                     IGLDAEQVKPTPEFGGVLNTEYIMGLGTVDDRMLILLDIEKLMSSTDMGLHD"
     gene            628..2877
                     /locus_tag="PK522_09745"
     CDS             628..2877
                     /locus_tag="PK522_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNC42053.1"
                     /translation="MLENLTIKSQLVILGIISTVLLAGVGSYGIYGVNQTNNKLKTVY
                     EDRTVPLADISGILDRAQRSRLNAVIASSYESLEEANKRAAMTVQRDSEINEIWKKYM
                     LTTLTPEESFLSNQFVERWKVFQEARNMAINYATQGDFKAAHTIAMNEINPKFDLVHE
                     TLFKLIQLQEDVAGKEYGLAQSQSSTIFTVTVLLVVLGVIFSGVLSILLIKNIMQSIR
                     QVVDVMDDVAAGQLDKKVLVQKQNELAPLLESMQRIVAVLKDFITEQKHMHKQHSNGL
                     MSYRIPVDKFSGGYAEVVKATNDLVSQHIAINRQVVEVISHYAQGDLSVEIGRLPGEM
                     VKISEVMDQVKTNLLSISNEIKLLLEGALAGDFSRRGDVSKYHHEFKQMITGLNQLMQ
                     INETGLGEVVRVLGALSRGDLTEQITSNYKGLFEKLRNDTNITVEQLTHIIRQIKESA
                     DSISTASQEIAQGNMDLSQRTEEQASNLEETASSMEELTSTVKQNAENAKQANQLAAS
                     ASDVAVKGGEVVGQVVETMSSINESSKKIVDIISVIDGIAFQTNILALNAAVEAARAG
                     EQGRGFAVVATEVRTLAQRSAAAAREIKSLIGDSVNKVENGTQLVDQAGRTMDEVVMS
                     VKRVTDIMAEISAASQEQSAGIEQVNLAISQMDEITQQNAALVEQAAAASESMHEQAA
                     QLAQAVAVFKLQHAAQAAKHQGEKKIDRRGPDRPGNVQRLPKTANQASKKETMPVAKT
                     GTDGDWEEF"
     gene            2925..>5004
                     /locus_tag="PK522_09750"
     CDS             2925..>5004
                     /locus_tag="PK522_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HNC42054.1"
                     /translation="MRLNLPVNDVEREMRDGEFLVSKTNLKGVITYVNQPFIEMSGYT
                     KEELIGQAHNLIRHPDMPPAAFEDFWKTLKQGRPWSGFVKNRAKDGSFYWVFANATPI
                     NEAGQITGYLSVRSKPTREQIRAAENLYREMREGRAKVKINAGDVVPLTLIGRLKQRL
                     RFVSVRTRIIMMLSFFSVIIICLGITGLLGFNQSNEALRSMHIDRTGALEDLSMILDK
                     IQQIQIHSHVVSSQNNLEVVKKRYAMVTEREKELDERWTEYLGTYLLPLEKELAETFH
                     SQMKAFREASAITLNAALNGDYERAYRNQINTVEPKFELAYTTLVKLIEFQAAAAKND
                     YELAVEKFTLGRNYGLFVIALSIFVSILGGYALLLAITRPLARLKRIAEKISGGNFTS
                     QITIANYDEIGSLTETIKTLQICSGSSLDEAQRTAAENFRIRTVLDQSTAAVMIADSA
                     NTIIYMNQTMRKALIAYQAEFQKVIQNFKPDAIVGNDFSYFQQSINLLGLTKPTKQTI
                     NIGERIYLLTLVPVLDGSGNRMGTSIEWLDRTIEVKVEQEIASVVGAAGEGDFSKRLS
                     LEGKEGFFAQLARNINNLVETSASGLSEVVRVLGALSKGDLTEKITNDYKGMFAKLKA
                     DSNATIEQLKHIIHKIKESADSISTASQEIAQGNMDLSQRTEAQASNLEETASSMEEL
                     TSTVKQNAENA"
BASE COUNT         1602 a          877 c         1252 g         1273 t
ORIGIN      
        1 aaacaagaaa tggtcaatgc ggcatgaccg gtagagagga agtaaagcca tgcaaatatt
       61 agcggaaagt aatcagggca tgtcagagtt ggcgcatgcc aatattgcac aagaatttct
      121 gacatttcgg ttaggaaatg aagaatacgg aattgagatt ttaaaggtac aagagattcg
      181 aggatacgac accatcacgc agattgccaa tgcaccggag ttcatcaaag gtgtagtgaa
      241 tttgcgaggg attatcgtac cgatcatcga catgcggatc aagtttaact tgggagaagc
      301 aacctatgac cggttcacgg tagtgatcat attgaactta tcgggtcgtg tggtggggat
      361 tgtggtggat ggggtatcgg atgtaattgg attggatgca gaacaggtaa agccgacacc
      421 ggaatttggg ggtgtactca acaccgaata catcatggga ttgggaacgg ttgatgatcg
      481 catgcttatc ctcctcgata tcgaaaaact catgagtagc accgatatgg gcttgcatga
      541 ttaacagagg tgtggcttga agcatccgct tagaagggaa cgttgtaggt tggcgcatca
      601 ttaaatttgt aaaaaaaaga ggttgatatg cttgaaaatt taaccattaa atcgcagctg
      661 gtgattcttg gaataatctc aacggtacta ctggctggcg taggcagtta cggtatctat
      721 ggggtcaatc aaacaaacaa taagcttaaa accgtttatg aagatagaac tgttccatta
      781 gcggatattt ctggaatctt ggatagggca cagcggtctc gattaaatgc ggtgattgca
      841 tcaagctacg aaagtttaga agaagccaat aaacgcgcag caatgacggt gcagcgggac
      901 tcagagatca atgagatctg gaaaaaatat atgctgacta cgctgacacc tgaagaaagt
      961 tttttaagta atcagttcgt tgagcgctgg aaagtatttc aagaggcgcg caacatggcc
     1021 atcaattatg caactcaagg cgattttaaa gcggcgcata cgatcgcaat gaacgagatc
     1081 aatcctaaat ttgatttggt gcatgagacc ttatttaaat tgattcagct acaagaggat
     1141 gtagcgggta aggaatatgg actggctcaa agtcaatctt ctacgatatt tacagtaacg
     1201 gtgctattag ttgtgctagg ggtaatcttc tcaggagtgc tgagtatttt gctgattaaa
     1261 aatatcatgc aatcgatcag acaagtcgtg gatgtgatgg atgatgtggc ggcaggtcag
     1321 ttggacaaga aagttttagt acaaaaacaa aatgaattag cgcctttgct ggaatcgatg
     1381 caacgtatcg ttgcggtatt aaaggatttc atcactgagc aaaagcatat gcataagcaa
     1441 cattcaaatg gtttgatgag ttatcgcata cccgttgata aatttagtgg tggttatgct
     1501 gaagtcgtca aagcaaccaa tgatttggtt tcgcaacata tagcgattaa tcggcaggtc
     1561 gtagaagtga tttctcatta tgcgcagggt gatttatctg tagagatcgg tcgattgcca
     1621 ggagaaatgg tcaagatatc tgaagtgatg gatcaagtaa aaacgaatct attatcgata
     1681 agcaatgaga tcaaattgct attagaaggt gcattggcag gagatttctc tcgacggggt
     1741 gatgtatcta aatatcatca cgaatttaaa caaatgatca ccgggctgaa tcagctcatg
     1801 caaatcaatg aaacgggcct gggtgaggtg gtaagagtac tcggtgcatt atccagaggg
     1861 gatttaaccg aacagattac gagtaattat aaagggctat ttgagaagct aagaaacgat
     1921 accaatatta ccgtcgagca attgacgcat attatccggc agattaaaga atccgcagat
     1981 tcgatcagca cggcttccca agaaattgcg caaggaaata tggatttatc gcaacggact
     2041 gaggagcaag cttctaactt ggaagaaacc gcttcgagca tggaagaatt gacttcgacg
     2101 gttaaacaaa atgcagagaa tgccaagcaa gcgaatcaat tagccgcatc ggcatcggat
     2161 gtcgcagtca aaggaggaga agtcgttggt caagtggtag aaacgatgag ttctatcaac
     2221 gaaagctcta agaagatcgt cgacattatc agtgttatcg atggcattgc gttccaaacc
     2281 aatattctgg cattgaatgc tgctgtggaa gctgctagag cgggagagca aggccggggg
     2341 tttgcagtgg ttgcaacaga agtacgtacg ctggcacagc gctcagctgc ggctgcgaga
     2401 gaaatcaagt ccttgattgg tgattcggtt aacaaagtag aaaatggaac ccagttggtt
     2461 gaccaagcag gaagaaccat ggatgaagtt gtaatgtcgg taaaacgggt gacggatatc
     2521 atggctgaaa tatcggccgc ttcccaagaa caaagtgcgg gtattgaaca ggttaattta
     2581 gccattagcc aaatggatga aatcacgcag cagaatgcag ccctggttga acaggcggct
     2641 gcggcatcgg aatcgatgca cgaacaggcc gcacaactgg cacaagctgt cgcagtcttt
     2701 aaattacaac acgcagcaca agctgcaaaa catcagggtg agaaaaaaat tgaccgccga
     2761 ggccctgatc gtcctgggaa tgttcagcga ttacctaaaa ccgctaatca ggcatcgaag
     2821 aaagaaacga tgccagttgc taagacgggt acagatgggg actgggaaga gttttgatgc
     2881 agaagatcga aaacatgata gctacgatta actgagaaca gactatgaga ttgaatcttc
     2941 cggtcaatga tgttgaaaga gaaatgagag atggagagtt tcttgtctct aagaccaatt
     3001 taaaaggtgt gattacttat gttaatcagc cttttataga aatgagtggt tatacgaaag
     3061 aagaactcat tgggcaagca cataacttga ttcggcaccc ggatatgccg cccgctgctt
     3121 tcgaggattt ttggaaaaca cttaaacaag gcaggccttg gagtggtttt gtaaagaatc
     3181 gcgcaaaaga cggatctttt tactgggttt ttgctaatgc tacgccaatt aacgaagctg
     3241 ggcagattac tggctatcta tcggtacgta gtaagccgac gcgtgaacaa attagagcgg
     3301 ctgaaaattt atatcgtgaa atgagggagg ggcgtgcgaa ggttaaaatt aatgcgggcg
     3361 acgttgttcc actgacatta atcgggcgat taaaacagcg gttacgattt gtaagtgttc
     3421 gtactcgtat catcatgatg ctcagttttt ttagcgtcat aattatctgc ctagggatta
     3481 ctggtttatt gggctttaat caaagtaatg aagcgcttcg ttccatgcat attgatcgta
     3541 ctggcgcatt agaagattta tcgatgattc tggataaaat ccagcaaatc caaattcata
     3601 gccatgttgt aagtagccaa aataacctag aagtcgtgaa aaaacgctat gctatggtta
     3661 ctgagcgtga gaaagagctt gatgagcgat ggacagagta tctgggcact tacctcttgc
     3721 cattagaaaa ggagctggct gaaacttttc actcgcaaat gaaagcgttc cgagaagcca
     3781 gtgcaataac ccttaacgca gctttaaatg gagattacga gcgagcgtat cgcaatcaaa
     3841 ttaacaccgt agagccgaaa tttgaattgg cttatacaac cttagtcaag ttaattgaat
     3901 ttcaagcagc agcggcgaaa aatgactatg agttagctgt cgagaagttt actttaggcc
     3961 gaaattatgg gttgtttgtg atcgccttgt cgatattcgt atcgatatta ggggggtatg
     4021 ccctgttact ggctattact cgacctttag caagattaaa gcggattgct gagaaaattt
     4081 ctgggggaaa ttttaccagc cagataacca ttgcaaatta tgatgaaatc ggaagtctta
     4141 ctgagacaat caaaacatta caaatttgtt ctggtagtag tttagatgag gcccaacgca
     4201 cagcagccga aaatttccga attagaacag tgttagatca gtctactgcg gcagtcatga
     4261 ttgctgacag cgcgaatacg attatttata tgaatcaaac gatgagaaag gcgctgattg
     4321 cataccaagc agaattccag aaagtaattc aaaattttaa gcccgatgca attgtaggca
     4381 atgactttag ctattttcag caatcaatca atctgcttgg ccttactaaa cccacaaaac
     4441 aaaccattaa tattggagaa agaatttatt tactaaccct agtaccagtg ctagatggtt
     4501 ctgggaatcg aatgggaacg tcaattgaat ggttagaccg aaccattgaa gtcaaagttg
     4561 agcaagaaat agccagtgtc gttggagctg caggggaagg tgattttagt aagcgtttat
     4621 cattagaagg taaagaagga ttttttgcgc aattagcaag aaatataaac aatctagttg
     4681 aaacgagtgc atctggctta agtgaagtgg tgagagtact tggtgcttta tcaaaaggtg
     4741 atctgactga aaaaattacc aatgactata aagggatgtt tgccaagtta aaggcggatt
     4801 caaacgcaac gattgagcag ttaaagcata ttatccataa aatcaaagaa tccgcagatt
     4861 cgatcagcac ggcttcccaa gaaattgcgc aagggaatat ggatttatcg caacgtacgg
     4921 aagcacaagc ttctaatctg gaagaaacag cttcgagcat ggaagaattg acttcgacgg
     4981 ttaaacaaaa tgcagagaat gcca
//
LOCUS       DAOQLJ010000156         4990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_7806_length_4990_cov_5.806890, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000156 DAOQLJ010000000
VERSION     DAOQLJ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7806_length_4990_cov_5.806890"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..638)
                     /gene="cysC"
                     /locus_tag="PK522_09755"
     CDS             complement(12..638)
                     /gene="cysC"
                     /locus_tag="PK522_09755"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNC42055.1"
                     /translation="MTTLPRVSSDILWHHASVTRHRRELLNGHRGAILWFTGFSGAGK
                     STLAHAVEEVLHQSGYKTFVLDGDNVRHGLCNDLGFSPENRVENIRRVGEVAKLFMEA
                     GIIVLTAFISPFRVERERVRNLVAQGDFIEIYCNSPIEICESRDVKGLYKKARAGQIA
                     EFTGISSPYEAPENPELTVNTSITDLNTSVQQVINELQSRNIIKKINE"
     gene            complement(680..2224)
                     /locus_tag="PK522_09760"
     CDS             complement(680..2224)
                     /locus_tag="PK522_09760"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNC42056.1"
                     /translation="MIQMILLLILTTLLAAISIRFAIIYALANNIADHPGGHKQHDTV
                     TPFVGGIGLLIAFSIALIVLFHNQPEFWIKWLALGIAAAIVFIIGLLDDIFHLNYKIR
                     FLAQGIVALIMIFMGGVQLSELGTLISNNQVLELGLFAIPLTMFATIGGINALNMIDG
                     IDGLAGTLSWITLLLISILAFFGGDQANTTLAIALVGGISGFLYFNLRYSTQNRARVF
                     LGDNGSMLLGFLFAWLLVDLSQGSHASMTPVTAIWLFAVPLMDTVTVMLRRIWLRQSP
                     FVPDHNHLHHILLHAGFRIEDTVFVIGTLHLILGSFGLVGLYLGIPESIMFFSFLVIY
                     TGYFYLTQHPWYIMSSLRHLHNFLGLLPTDDYGVFLGSYSAREAETLVKMVCKELGPS
                     VDSWVHVIEQQSSNNYAEKRYSVAVNIRLSNTDYVTDKEISQYIAKLSHRMMERWGIR
                     VRQFVKRNIKNDRRIKDIGNPLGGNFRVSERRSIEIPILMFEAMFDKLAIERNNHSEE
                     SISETR"
     assembly_gap    2537..2636
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2637..3232)
                     /locus_tag="PK522_09765"
     CDS             complement(<2637..3232)
                     /locus_tag="PK522_09765"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNC42057.1"
                     /translation="MPDTIVDLIRHGEPAGGQRYRGHRVDDPLTEKGWSQMWNSVGEY
                     QKWTHIITSPLQRCNAFALSLGKRLNIPVIVESRFKEVGFGLWEGLSHDEIRNNRAEE
                     FQAFITDPVNCRPQGAEPLDTFLQRVNLAYEDIIKLNQNHHYLIVAHAGVIRAIIART
                     VHLAPSGFYRIKISNGGVTRIRHTTTGPILELLNGKLAG"
     gene            3577..4554
                     /locus_tag="PK522_09770"
     CDS             3577..4554
                     /locus_tag="PK522_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNC42058.1"
                     /translation="MKKLIIICGMPRSGTTWLGKIFDSHPGILYRHEPDAGGKLNLLP
                     LFTETWVEEELKFIKQFISELESHTRVNQVSKFPIFKKIRESDIHFQVRKAYLTALKI
                     ASRYFGNFSAYEMLPNQSDMTIVWKSIESLGRMPIMAQAVQELGIEAYFIHIIRHPCG
                     QLNSTLRGQAKQVFTGNYNAAEDEPLFKLLASSSQAQLYELTVSQFQQMNAEQRLTWR
                     WALMNDFALRLMENKPNYFKVRYEDICQEPELTTKELFTFCKLGWNEQTAHFLEMSTK
                     QDVPDYYSVIKNPLNSANAWRKKLSEQQINDIFAVSEKTLSGKLYLPRD"
     gene            complement(4592..>4990)
                     /locus_tag="PK522_09775"
     CDS             complement(4592..>4990)
                     /locus_tag="PK522_09775"
                     /inference="COORDINATES: protein motif:HMM:NF017475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HNC42059.1"
                     /translation="LMTRIGVLIAIALVMTIAVYGLVAVIIKLDDLGLWLKKKSDATL
                     TIIGQFLLNATPWLMRFLSVAGTVAMFLVGGNILMHGIPSFAHWIEPIISSTNGLSTL
                     TISSLFNILLGIMCGGLILGITRVIQKIKS"
BASE COUNT         1548 a          939 c          990 g         1413 t
ORIGIN      
        1 ttttagaaag actattcgtt aattttttta atgatattcc ttgattgcaa ttcattaatg
       61 acttgttgaa cggaagtatt cagatcggta atgcttgtat taacagtcag ctctggattc
      121 tctggtgctt catagggaga agaaatacct gtaaattctg cgatttgacc tgctctggct
      181 tttttataga gtcctttaac atctcgagat tcacaaattt caattgggct attgcaataa
      241 atttcaataa aatcaccttg cgcaactaga tttcttactc gctctcgttc tacgcgaaat
      301 ggggaaataa aagcagttag cacgataatt cctgcttcca tgaatagttt tgcgacttca
      361 cctactcggc ggatattttc tacgcgattt tcaggagaga atccaagatc gttgcataac
      421 ccatgccgaa cattatcgcc gtcgagtaca aacgttttat acccgctttg atgaagcact
      481 tcttcaacag catgggccaa ggtagacttt ccagcgccag aaaacccagt aaaccagaga
      541 atagccccgc gatggccatt cagtaattct cgtcggtgac gagttactga agcatggtgc
      601 cagagaatat cgcttgatac tcggggtaac gttgtcatga ttactcctga aatcgttgaa
      661 atgttatagc ttagcttcct tagcgtgttt ctgaaattga ttcttctgaa tgattgttgc
      721 gctctatcgc aagtttgtcg aacattgctt caaacatcag tatcgggatc tcaattgaac
      781 gtctctcaga aacacgaaag ttgccgccca aaggattgcc gatatctttg attcttcggt
      841 catttttgat attacgttta acaaattgcc ggacacgaat gccccagcgt tccatcatgc
      901 ggtgtgacag ttttgcaata tactgactaa tttctttatc agtgacataa tctgtgttcg
      961 ataatcggat attaactgcg actgaatatc ttttttcagc ataattattt gatgattgtt
     1021 gttcaatgac atgcacccag gaatcaacgc ttggtccaag ttctttacag accattttaa
     1081 ctaatgtttc agcttctcgc gcagaataac tacccaagaa aacaccgtaa tcatcagtgg
     1141 ggagtaaccc taagaaattg tgtagatggc gtaacgatga catgatatac catggatgct
     1201 gtgttaagta aaaatagccg gtgtatatca ctaagaaact aaagaacata atagattcgg
     1261 gaatacccag atacaaaccc actaagccaa atgatcctag tattaagtga agcgtgccaa
     1321 taacaaaaac ggtatcttct atcctaaaac ctgcgtgtag caggatatga tgtaaatgat
     1381 tatgatcagg aacaaatgga gattggcgta accaaattct gcgcagcatc acagttacgg
     1441 tatccataag cggaacagca aatagccaaa ttgctgttac aggagtcatg gaggcatgag
     1501 atccttgtga aagatcgacc aatagccaag caaataaaaa accaaggagc atactcccgt
     1561 tatccccaag aaagacacga gcacgattct gtgtggagta gcggaggtta aaatataaga
     1621 aaccagaaat acctccaact aatgcgatag caagcgtggt gttggcctga tctcccccaa
     1681 aaaacgccaa gatactgatc agtagtaaag taatccatga tagcgtacca gcaagtccat
     1741 caatgccatc gatcatattg agtgcattga ttcctccgat agtagcaaac attgtaaggg
     1801 gaatggcaaa tagacctaat tccagaactt ggttattaga aattaatgtt ccaagctcgc
     1861 tgagttgtac tcctcccata aagatcatga tcagcgcgac tattccttga gcaagaaatc
     1921 ttattttata gttgagatga aagatatcat caaggagacc tataatgaaa acgatagccg
     1981 cagctattcc aagtgctaac cattttatcc agaactctgg ttgattatgg aataaaacga
     2041 ttaaagcaat agaaaatgca atgagtaacc ctattccgcc gacaaaaggg gtcacggtat
     2101 catgttgctt gtggccaccc gggtgatcag cgatgttatt tgctagtgca taaattattg
     2161 caaatcgaat actaatcgct gctaataaag ttgttagtat gagcaaaaga atcatttgta
     2221 tcataatcag attacccctt aagaattgat agatttctaa tcagattggg ggaagaactt
     2281 agcatagata aatgaaggaa atttgaatcc ttaaaacgca cccgatcatt ttataatatt
     2341 atcaataaga tagcattata aatttcttta gcatacaaca aatgattgaa ataattatat
     2401 taattcctat cagtaaggat atcaatttac gctattctga tgctatcgaa agaagttaag
     2461 ccaaagtaat ggaaactaga attttgtatt tttacatata ccgcaaaatc agagaatgcg
     2521 gcaaattgtc acacccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncctg
     2641 cgagtttccc atttaataat tctagaatcg ggcctgttgt agtgtgacga atgcgtgtaa
     2701 cgccaccatt actaattttt attcgataga aacctgaagg tgcgagatgc acggttcttg
     2761 caatgattgc acggataacc cctgcatggg ctacgatgag atagtgatga ttttgattaa
     2821 gtttaataat atcctcatag gctaaattga cccgttgcag aaaagtatct agaggttcag
     2881 cgccttgcgg cctacaattg acagggtctg tgataaatgc ctgaaattct tctgcgcgat
     2941 tatttctgat ttcgtcatga ctcaatcctt cccaaagtcc aaagccaact tctttaaagc
     3001 gtgattcgac aataaccggt atgttaagtc gcttacctaa tgaaagagca aacgcattac
     3061 agcgctgtaa tggagacgta ataatatgtg tccatttctg atattctcca acggaattcc
     3121 acatctgtga ccaacctttt tccgtcagtg gatcatcgac acgatgacca cggtaacgtt
     3181 gacctcctgc tggctcgcca tggcgaatca aatcaacaat ggtatcaggc attaacataa
     3241 tcggtactcg taatgtggcg taaattaaat agcgctaaat ttttgtatgc ttttatttgc
     3301 aatctatcat aaagaaatat tgaactgaag caattgagtg agaagcaatt tccttttaaa
     3361 ggagcagcca ggtaacctgt aaaaaataat gtttactttt tagaggcttg atttgcagat
     3421 gtgttaagtg tttaaaaagg cgttactctt gtggttttta tcattctata aagaaaaact
     3481 gtcgtgtatg atgcatgtgg ccctaaagca agccatgcca aatagttgag tactgattga
     3541 tatattgatg ctaaatcatc ccgaataggc tagttaatga aaaaattgat tattatctgc
     3601 ggaatgccgc gctcagggac aacctggcta ggtaagatat ttgatagcca ccctggaatt
     3661 ttatatcggc atgaacccga tgccggcgga aagctaaatt tattaccact ctttactgaa
     3721 acctgggttg aggaagaact taaattcatt aaacaattca tttccgagct tgaatcgcac
     3781 acgcgggtta atcaagtaag caagtttcca atcttcaaga aaattcgtga aagtgatatc
     3841 cattttcaag tgcgtaaagc ctatctcacg gcattaaaga ttgcttcacg atattttggt
     3901 aatttttcag cttatgaaat gctacctaat caatcggata tgacgattgt ttggaaaagt
     3961 attgaatcgc tggggcgaat gcctataatg gcgcaagctg tccaagaact aggaattgaa
     4021 gcctatttta ttcacatcat tcgtcatccg tgcgggcaac taaactcaac attaagaggt
     4081 caagcaaagc aggtatttac agggaattat aatgctgctg aagatgagcc gctttttaaa
     4141 ttgcttgcaa gttccagtca agcccagttg tacgagctaa cagtgtccca atttcaacaa
     4201 atgaatgcgg agcaacgttt aacgtggaga tgggcgctca tgaatgattt tgcgttgcgt
     4261 ttaatggaaa ataaaccgaa ttattttaag gttcgatatg aagatatctg ccaggagcct
     4321 gagctgacta cgaaggagtt atttacattt tgtaaattgg gatggaatga gcaaacagcc
     4381 cactttcttg aaatgagcac caagcaggat gtgcctgatt actatagtgt tatcaaaaat
     4441 ccacttaatt ccgcaaatgc ctggcgaaaa aaattgagtg agcagcagat taacgatatt
     4501 tttgcggtat ctgaaaaaac gttgagcggg aaactctact tgccgagaga ttaaattttg
     4561 ttagtgaatt gtaattagct aggttgtttt tttatgattt gattttctga atgacgcgtg
     4621 tgattcctaa tataagacca ccacacataa tgcctaatag aatattgaat agtgatgaga
     4681 tggttaaagt agatagacca ttggtcgagg aaataatggg ttcaatccag tgagcgaaac
     4741 tgggtattcc atgcatcaga atattgccac ccactagaaa catcgctacg gttccagcga
     4801 ccgataaaaa ccgcattaac caaggtgttg cattgagaag aaactgcccg atgatggtga
     4861 gtgtagcgtc gctctttttc tttagccaga gcccaagatc atctaattta ataatgactg
     4921 caactaaccc ataaaccgca atcgtcatga ccaatgcgat ggcgattagt acgccgatac
     4981 gtgtcatgag
//
LOCUS       DAOQLJ010000157         4871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_8126_length_4871_cov_6.423796, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000157 DAOQLJ010000000
VERSION     DAOQLJ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8126_length_4871_cov_6.423796"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1500)
                     /gene="gltX"
                     /locus_tag="PK522_09780"
     CDS             complement(115..1500)
                     /gene="gltX"
                     /locus_tag="PK522_09780"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNC42060.1"
                     /translation="MVRTRFAPSPTGYLHIGGARTALFSWAFARKHGGKFVLRIEDTD
                     LERSTQESVQAILDGMAWLNLDYDEGPYYQMQRLTRYQEVAEQLLRTNQAYYCYATKE
                     ELDLLREGQRAAGQKPRYDGRWRDNKSIPPQGIKPVIRLKNPLEGEVVFDDLVKGRIS
                     VANNELDDLVLMRSDGTPTYNFGVVLDDLDMQISHVIRGDDHVNNTPRQINILKALSA
                     SLPQYAHVPMILGADGERLSKRHGAVSVMHYHEEGYLPKALVNYLARLGWSHGDDEIF
                     SSEQLIEWFDLESINRAPAKFNPEKLQWLNQHYLKTEAITDIAHLTIPFLEKDNCEIS
                     NREYLHKVVSLLKDRVSTISELADAAVYFFRVLAPSEELRAQYFNNETKSILVSLIDR
                     FSSISWDKQLIHDEIKNTATAFGVKLPKIAMPLRVAITGEVHTPSIDAVLEILGKSET
                     LNRLNRQITTL"
     gene            1830..3446
                     /gene="nadB"
                     /locus_tag="PK522_09785"
     CDS             1830..3446
                     /gene="nadB"
                     /locus_tag="PK522_09785"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNC42061.1"
                     /translation="MQVHFDTLIIGSGLAGLTLALNLANTQKVAIVTKRTLLDSASSW
                     AQGGIAAALSTEDSPDKHIQDTLIAGAGLCDTAITKYVVEQGPAAIQWLISQQVDFTK
                     DAQNETGYHLTREGGHSVRRVIHINDATGQAVQLALIRQTQAHKNISIFENHIAVDLI
                     TNKKLLANPLNQLNRCHGAYILDIKSDKIITFSAQNTVLATGGAGKVYLYTTNPDTAT
                     GDGIAMGWRAGCRVANMEFIQFHPTCLYHPHAKSFLISEAVRGEGGLLKLPNGERFMP
                     SHDERAELAPRDIVARAIDFEMKKRGLDCVYLDISHKPISFLKQHFPTIYSRCLDFGI
                     DITKQPIPVVPAAHYMCGGIITDQNGMTDLNNLYAIGETAFTGLHGANRLASNSLLEC
                     LVFGMASAKHILDQPNQESAALPDWDASRVTDADEEVVISHNWDELRRFMWNYVGIVR
                     TNKRLERAQHRVKLLHEEINEYYTNFRVTGDLLELRNLVTTANLIIQCAMQRHESRGL
                     HSSRDYPNTSSELKNTILQGRGAVGNHLSY"
     gene            complement(3466..4800)
                     /locus_tag="PK522_09790"
     CDS             complement(3466..4800)
                     /locus_tag="PK522_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HNC42062.1"
                     /translation="MYLDTDIKSSDFLPNNHPQRIILHNEVHARPSQRIHLPALIIYV
                     AVFNEGVTREQECNHLRLLPNQANLTPEQLQNNFLRLRLQGYALRWERHTEYTRYTVV
                     QQLPDSANLGATDPELLSYLALPKNWLAGIPGLTIAAIKLAMVHGDLTQMEDLLKTAR
                     SWFGGHPIVASLMGRDGHSLAVTDFMLRESGFERMLVIAPPSTSETRAGRISQRLLEI
                     ETYRLVALKALPVAKQLAPELTDAELQLADITARLESKSTSDQELLDTLIALAARVER
                     ITVEHAYRFAATHAYDRVVNQRISELREKAIPGIQTIGEFMQRRLSPAIATVDATAQR
                     MGYLSARVARTSSLLRTRVDILAETQNQQLLEKLTRGQELQLRLQSTVEGLSIAAISY
                     YVISLLLYGFKAIKAAGWPIHPELVTGLLIPVVIWSVWYITHRIHKKFLESH"
BASE COUNT         1453 a         1087 c          935 g         1396 t
ORIGIN      
        1 ccgctgacct cttgcatgcc atgcaagcgc tctcccagct gagctatacc ccctagactg
       61 aaagaatagg cgattatact gacgctaaat atccttgtaa agcgatagat aaatttacaa
      121 agtcgtaatt tgccgattta atcgattcaa tgtctccgat tttccaagta tctcaagtac
      181 cgcatcaatc gaaggtgtat ggacttcacc ggtaattgca actctcaagg gcattgcaat
      241 cttaggcagt ttaacaccaa acgctgtcgc tgtattttta atttcgtcat gaatcaattg
      301 tttatcccag ctaatcgaag aaaatcgatc gattagagaa accagaattg attttgtttc
      361 attattgaaa tattgcgctc tcaactcttc agaaggcgct aatactcgaa aaaaataaac
      421 agcagcatca gcaagttcac ttatcgtact cactcgatct ttgagtaaac tcactacttt
      481 atgcaagtat tcacgattag aaatttcaca attatctttt tctagaaaag gaatggttaa
      541 atgagcaata tcagtaatag cttctgtttt gaggtaatgc tgattaagcc actgcaattt
      601 ttctggatta aatttagctg gcgcacgatt gattgattca agatcaaacc attcaatcaa
      661 ttgttcacta gaaaaaattt catcatcacc atgagaccat cctaaacggg ccagatagtt
      721 taccaacgct ttaggcaaat aaccctcttc atgataatgc atcactgata ccgcgccatg
      781 acgtttagaa agccgctcac catctgcacc taaaatcatc ggcacatgtg catactgagg
      841 taacgatgca ctaagcgcct tcaaaatatt gatctgacgc ggtgtattat tgacatgatc
      901 atctcctcga atcacatggc taatttgcat atcaagatca tccaatacaa ccccaaaatt
      961 ataggtcggt gtcccatcac ttctcattag cactagatca tcaagttcgt tattcgcaac
     1021 actaatcctg cctttgacta aatcatcaaa gacaacttct ccctctagcg gatttttcaa
     1081 tcgaatcacc ggctttatcc cctgcggtgg tattgactta ttatcgcgcc aacgaccatc
     1141 atagcggggt ttttgtccag ctgctcgctg accctctctt aataaatcaa gttcttcttt
     1201 ggttgcataa caataatacg cttgattagt tcgcaatagc tgttcagcaa cttcttgata
     1261 acgagtcaat cgttgcatct gataataagg gccctcgtca taatctaaat ttaaccaggc
     1321 catgccatct aaaatcgctt gtacagattc ttgcgttgag cgctctagat ctgtgtcttc
     1381 aatacgtaaa acaaatttac caccatgctt acgtgcaaat gcccatgaaa acaatgcagt
     1441 acgcgcacca ccaatatgga gataccctgt agggcttgga gcaaatcgag ttcgaaccat
     1501 cataagatta tttcagatca aaaaactagt gattttgtta gagattttaa aaaataatgt
     1561 cagggtaaaa aatcaggtag attttcagtt taactggtag atattcaccc taattccatt
     1621 cacacgatca aagaacagga gcaagcgatt aatcgtcagc taattctact acacccttag
     1681 caacctcgct tgtattagaa tgctctagca cttagccacg cgataacgca aaaaatgtta
     1741 tagggtatat cagcaaacgc agttttcctc tcgtgctaga ataaaaacaa cagtagctct
     1801 atatttagtt aataaataat aataaacata tgcaagttca ttttgatacg ctcatcattg
     1861 ggagcggctt ggctggactc acgctcgcac ttaatttagc aaatactcag aaagttgcta
     1921 ttgtcacaaa acggactctg cttgatagtg caagtagctg ggctcaaggt ggtattgccg
     1981 ctgccttatc tactgaagat tctcccgaca aacacatcca agatacgctt attgcgggtg
     2041 ctggactatg cgataccgca ataacgaaat atgtcgtcga acaaggccct gcagccattc
     2101 agtggctaat ttcacaacag gtcgatttca ccaaagatgc tcaaaatgaa actgggtacc
     2161 atttaacgcg cgaagggggg cacagtgttc gtcgcgtcat ccatatcaac gatgcaacag
     2221 gtcaggcagt tcaacttgct ttaattcgac agacccaagc acataaaaat atttcgatct
     2281 tcgagaatca tatcgcggtt gacttgatta caaataaaaa attattagcc aatccattaa
     2341 atcaactgaa tcgatgccat ggcgcttata tcttagatat caaatcagat aaaatcatca
     2401 cattctcggc acaaaacacg gttcttgcaa cagggggggc gggcaaagtt tatctttaca
     2461 ccactaaccc ggataccgca acgggtgatg gcatcgccat gggatggcgg gcaggatgcc
     2521 gcgtagctaa catggaattc atccaatttc atccaacgtg tctttaccac cctcatgcaa
     2581 aatcattttt aatcagtgaa gcggttcgcg gtgaaggcgg tttattaaag ctacccaatg
     2641 gtgaacgctt tatgccttca catgatgaac gtgcagaact tgctcctcgc gatattgtcg
     2701 ctcgcgccat cgattttgaa atgaagaagc gtggccttga ttgtgtctat cttgatatct
     2761 cccataaacc aatctcattt cttaaacagc acttcccaac gatttattca cgatgccttg
     2821 attttggtat tgatatcaca aaacaaccga ttccggttgt accagcagcg cattatatgt
     2881 gtgggggtat cataacagac caaaatggaa tgactgattt gaataactta tatgcaattg
     2941 gtgaaactgc atttactggt cttcatggcg ccaatcgatt agcaagcaac tccttactag
     3001 agtgtttagt attcggaatg gcctcagcaa aacatatatt agatcaacct aaccaggaat
     3061 ctgctgcctt gccggattgg gatgcaagtc gtgtaactga tgcggatgag gaagtcgtaa
     3121 tttcccataa ttgggatgaa ttacgtcgat tcatgtggaa ttatgtagga attgttagaa
     3181 ccaacaagcg ccttgaacgc gcgcaacatc gggtcaaatt gctgcatgaa gaaatcaacg
     3241 agtactacac gaatttccgt gtaaccggtg atttattaga gctacgtaat ttagttacaa
     3301 cagcgaatct gatcatccaa tgcgccatgc aacgccatga aagccgtgga ctacattcta
     3361 gtcgtgatta tcctaataca agctctgaat taaaaaatac gattttacag ggccgtggcg
     3421 ccgttggaaa tcatctaagt tattagcttt aaggatcata gaaggttaat gtgattctaa
     3481 gaatttttta tgaattcggt gtgttatata ccacacagac caaatgacca ctggaattag
     3541 taatcccgtt accaattctg gatgaatcgg ccatcctgca gcttttattg ctttgaaccc
     3601 ataaagcaac aaactaatta cataatagga aattgcagca atcgatagtc cttctactgt
     3661 actttgcagg cgtaactgca attcctgccc acgtgttaat ttttccaata gttgttgatt
     3721 ttgagtctca gctaaaatat cgactcgtgt ccgtaacaaa gaactcgtcc ttgcaactcg
     3781 agctgaaaga taacccatgc gctgcgctgt tgcatccact gttgctatcg ctggagaaag
     3841 acgtcgctgc atgaactctc caatcgtttg gataccaggt atcgctttct ctcttaattc
     3901 actgattctt tgatttacta ctcgatcata agcgtgagtt gcagcaaaac gataagcatg
     3961 ctcaacggta atacgttcca ctctagccgc aagcgctatt agtgtatcta gtaattcctg
     4021 atctgaagtc gacttactct ccagtcgtgc agtaatatca gccagttgta gctcagcatc
     4081 agtcaactcg ggagccaatt gtttggcaac gggaagcgct tttagtgcaa ctagtcggta
     4141 cgtctcaatt tcaagtaagc gttgtgaaat tctccccgca cgcgtttcag atgtactcgg
     4201 tggtgcaatg acaagcattc tttcaaagcc gctctcacgc agcatgaaat cagttactgc
     4261 aagtgaatgg ccatctcttc ccatcaagga agccacaatg ggatgtcctc caaaccaact
     4321 gcgcgcagtc ttcaacagat cttccatttg cgttaaatca ccatgcacca ttgcaagttt
     4381 aattgcagca atcgtcaatc ctggaattcc ggccaaccaa tttttaggta aagccaaata
     4441 agataacaat tctggatcgg ttgcacctaa attggcgcta tccggcaact gctgcactac
     4501 tgtataacgc gtgtattcag tatgcctctc ccatctcaag gcatatcctt gaagccgcaa
     4561 gcgtaaaaaa ttattttgca attgctcagg cgttaagttt gcctgattcg gcaataagcg
     4621 taagtgatta cattcttgtt ctcgtgttac accttcatta aaaactgcaa cataaatgat
     4681 aagtgcagga agatgaatcc gctgacttgg acgtgcatgt acttcattat gcagtattat
     4741 tcgttgcggg tgattatttg gtaaaaagtc tgatgatttg atatcagtgt ctaggtacat
     4801 cgttccaggc ttatgaacag cgagctatgt tttttttaaa tgtatcaaga atctgacata
     4861 aggtcaaaag t
//
LOCUS       DAOQLJ010000158         4849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_8174_length_4849_cov_6.501669, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000158 DAOQLJ010000000
VERSION     DAOQLJ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8174_length_4849_cov_6.501669"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PK522_09795"
     CDS             complement(<1..976)
                     /locus_tag="PK522_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42063.1"
                     /translation="MTFKSRLMKSILNIQSPHVDSDSDSNVVLLNPSEKLNGDLTYKH
                     CVDSENYELLSAGNSIPPQLSAETAFQEAKKRIEADAKASVAAEARARAEASARLAAE
                     LRIKAETAATEEARARAKAEALASKEAQARKELEAKLRQTIEDGIKAEKEIVATLRAK
                     VQAETLIAEKTRVRAKEEALILDQIKAKEEAEERAIEAAIVRRKIEEEARLLALSATE
                     AEAKAAAIARVKIQEEEKLLALAHAKTETERQALDEVRLRAEIEAKALAKAQEHLTAE
                     TKSREAEEAKLYAEIQAEQVTQAKIDAELNAKTAAELRAEADAKAAEAA"
     gene            complement(1150..1953)
                     /gene="fliR"
                     /locus_tag="PK522_09800"
     CDS             complement(1150..1953)
                     /gene="fliR"
                     /locus_tag="PK522_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNC42064.1"
                     /translation="MISFTYAELSTLLASFFWPLSRILAFISSAPILSHRSIPIQVKI
                     GFALLLTLTIVPTLQTLPSVDPGSEIGFLILMQQIVIGIAMGFAMRVIFVAIEMAGEI
                     IGLQMGLGFALFFDPQNSGQVQLIGRFLGLIALLVFLAIDGHLQMIAILAQSFSVLPI
                     GTAGMPSLSFSILANWGSEIFLLGMKLSLPILTTLLIANLALGILTRAAPQLNIFAVG
                     FPLTLAIGLAMMSLVLPYYLPILEQMFQNGLQIMLEIITPSTIEPSPTE"
     gene            complement(1987..2259)
                     /gene="fliQ"
                     /locus_tag="PK522_09805"
     CDS             complement(1987..2259)
                     /gene="fliQ"
                     /locus_tag="PK522_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNC42065.1"
                     /translation="MSPESAITIGRHALEITFMVSAPLLLAALVTGLIVSIFQAATQI
                     NEMTLSFIPKLLIMFAVMVLAGPWMLSVMMDYVQRLYANIPWLTTG"
     gene            complement(2272..3051)
                     /gene="fliP"
                     /locus_tag="PK522_09810"
     CDS             complement(2272..3051)
                     /gene="fliP"
                     /locus_tag="PK522_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635079.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNC42066.1"
                     /translation="MTDKRQAIVTWHRSLLFLLLITLCLMVLPVYAQQSNFPAFTSTP
                     TQNGGTAYTLSIQTLLLFTTLTFIPALILMMSSFTRIIIVLSLLRLALGTQSSPPNQV
                     LIGLTLFLTLFVMSPVIDRVYSEAYVPFSQDKINLFEAGEKASVPLKAFMLRQTREAD
                     LALFTQISNSEEIESRDQVPLKILVPAYITSELKTAFQIGFIVFIPFLIIDMVVASVL
                     MAMGMMMLSPMIISLPFKLMLFVLVDGWHLLIGSLTQSFYT"
     gene            complement(3079..3492)
                     /gene="fliO"
                     /locus_tag="PK522_09815"
     CDS             complement(3079..3492)
                     /gene="fliO"
                     /locus_tag="PK522_09815"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNC42067.1"
                     /translation="MNLQVPLRAEEVAKPEIAAMPSLVTGSSVLHMFLALLLVLAVIG
                     VIAWLLKRFGIGPGNQSNLIKVVAAAAVGQRERIVIVEIANAWLVLGVAPGHINLLHQ
                     IEKIASSTGSEQLSTEGTNDFFAQLQKNIKKQQDE"
     gene            complement(3528..3986)
                     /gene="fliN"
                     /locus_tag="PK522_09820"
     CDS             complement(3528..3986)
                     /gene="fliN"
                     /locus_tag="PK522_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNC42068.1"
                     /translation="MSETEQTEKPIEADDWAQAMEEQSSTASDPAAIPESNHQSISSD
                     TLTASTPVFEQFSKDNVLNNAKNDIDIILDIPVQLTVELGRTRIAIRNLLQLAQGSIV
                     ELDGMAGEPMDVLVNGCLIAQGEIVVVNEKFGIRLTDIITPSERIRKLNR"
     gene            complement(3990..>4849)
                     /gene="fliM"
                     /locus_tag="PK522_09825"
     CDS             complement(3990..>4849)
                     /gene="fliM"
                     /locus_tag="PK522_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNC42069.1"
                     /translation="ERIVRGRMPTYEIINARFARFLQIGLYNFMHRAADISPGTMKTV
                     KYSEFIRSLVVPTNMNLVSMKPLRGTALIVFDPKLIFLIIDNLFGGDGRFHWRVEGRE
                     FTLTEQRIISRLLEVVYEDYQKSWKSVYPIDVEQVRTEMNPQFASIATPGEIVIATTF
                     TVDIGGTGGEFHTCIPYTMLEPIRDTLSSTLQGDQLELDKRWVRSLTKQVQSAEVELI
                     ANIGHAKITLNQILNMQEGDVVALDIPDTVTALADGVPLLECQYGVLNGQYALKVKSI
                     LSHSENSTE"
BASE COUNT         1421 a         1076 c          913 g         1439 t
ORIGIN      
        1 ttgctgcctc tgctgccttt gcatcagctt ctgcacgtaa ttctgctgca gttttagcat
       61 ttaactcagc gtcgatcttt gcctgagtta cttgctcagc ttgaatttca gcatacaatt
      121 ttgcttcttc agcttctctc gatttagttt cagctgtcaa atgttcttgc gcttttgcaa
      181 gtgcttttgc ttcaatttct gcacgcaagc gtacttcatc caatgcttga cgttcagtct
      241 ctgttttagc atgtgccaat gctaataact tttcttcttc ttgaattttt actctagcta
      301 tcgcagctgc tttagcttct gcctcagttg cagaaagtgc taataatctc gcttcttcct
      361 caatttttct tcttacgatt gcggcttcga tcgctctctc ttcagcttct tcttttgctt
      421 taatttgatc aaggatcagt gcctcttctt ttgctcgaac acgtgttttc tcagcaataa
      481 gtgtttcagc ttgaactttt gctcggagcg tagcaacaat ctctttctcc gctttgattc
      541 catcttcaat agtttgtcgt aattttgctt caagctcttt acgtgcttga gcttctttgc
      601 tagcaagcgc ttcagctttt gctctggcac gcgcttcttc tgttgcagct gtttctgctt
      661 taattctgag ctcagcagct aatcgtgcgc tggcttctgc acgagctcta gcctcagctg
      721 cgacagaagc tttagcatca gcttcaattc gtttttttgc ttcctgaaaa gcagtctcag
      781 cactcaactg gggagggata ctattccctg ctgataataa ttcataattt tctgaatcaa
      841 cacaatgctt ataggttagg tctccattaa gcttttcact cggattaagc aaaacaacgt
      901 ttgaatcaga atctgagtca acatgaggag attgaatatt taggatagac ttcatcaaac
      961 gagatttaaa tgtcatggca gctcaccgca caaaaagtgg aatgtttatc aaatacttag
     1021 gaagtaatgc acgattaaat taaatactta agtattacag tatcaatttc tttagcaaaa
     1081 caattgttag aagaaagggg gaaatactat ctgtttacaa aattagcttt cctaaaacca
     1141 cgcctaacgc tattcagtgg gcgatggctc tatagttgac ggcgttatta tttccagcat
     1201 gatctgcaat ccattctgga acatctgctc aaggataggt aaataataag gcaacaccaa
     1261 cgacatcatc gctaaaccaa ttgctaacgt tagtggaaaa ccaaccgcaa aaatattcaa
     1321 ctgtggagca gctctcgtca gaatgcctaa tgccaagttt gcaatgagta gtgtcgttaa
     1381 gatcgggagt gataatttca tcccgagtaa aaatatttca cttccccagt ttgccaaaat
     1441 gctgaatgat agcgaaggca tgcccgctgt ccctattggt aaaacgctaa aactttgtgc
     1501 aagaatggca atcatttgca agtgaccatc aattgcaaga aaaaccaaga gggcaatcaa
     1561 ccctaaaaat cgccctatca attgaacttg acccgagttc tgtggatcga aaaaaagggc
     1621 aaagcccagc cccatttgta aaccgattat ttcacccgcc atctcgatcg caacaaagat
     1681 aactcgcata gcaaaaccca ttgctatgcc aataacaatt tgctgcatca agatcaagaa
     1741 accgatttca gaacctggat ctaccgaagg caacgtttgt aaggttggaa cgatagtcag
     1801 agtcaacagg agtgcaaaac ctattttgac ttgtatcgga atactgcgat gactgagaat
     1861 gggtgcactt gagataaacg caagaattct actaagtggc cagaaaaaac tcgctaataa
     1921 tgtgctgagc tcggcataag taaagctgat catgacctga atcagtaatt gtaatagctt
     1981 ctacaactaa cccgtcgtca gccaagggat attagcatac aatctttgca cgtaatccat
     2041 cataacactt agcatccaag gccctgctaa taccatcact gcaaacataa tcaaaagttt
     2101 ggggatgaac gatagcgtca tttcattaat ttgcgtcgct gcctgaaaaa tgcttacgat
     2161 caaacctgtt accaacgctg cgagtaatag cggcgcagaa accataaatg taatttctag
     2221 cgcgtgccta ccgatggtaa ttgcgctttc tggactcatt gcataattcc tttaagtgta
     2281 aaaactttgt gtcagcgaac cgattagtaa atgccaacca tctaccaata caaacaacat
     2341 taatttaaat ggaagtgaaa tgatcatggg cgataacatc atcatcccca ttgccatcaa
     2401 cacgctggct acaaccatat caataattag aaacggtata aaaacaatga agccgatttg
     2461 aaaagcagtt ttaagttcac tcgttatata cgctgggacc aaaatcttta atggaacctg
     2521 atcgcggctt tcaatttcct cgctattcga tatttgtgta aataatgcca aatctgcttc
     2581 tcttgtttgg cggagcataa aagcttttag gggtacactc gctttttcac cggcttcaaa
     2641 tagattaatt ttatcttgtg aaaaaggcac atacgcttcg ctataaactc gatcaatgac
     2701 aggcgacatc acaaacagcg taagaaataa cgttaacccg attaaaactt ggttaggcgg
     2761 tgaagattgc gtaccaagtg caagccgtag caaggataat acgatgatga tccgcgtaaa
     2821 actcgacatc atcaggatga gcgcaggtat aaatgttaaa gtcgtgaata gaagaagcgt
     2881 ctggatactt aatgtatagg cggttccacc attctgagtc ggtgtgctgg taaatgctgg
     2941 aaaatttgat tgctgtgcat aaacaggtaa aaccatcagg caaagcgtga ttaacaataa
     3001 aaacaataac gatcggtgcc aggtaacgat tgcttgtctt ttatcagtca ttattttcac
     3061 ccattcacaa tacgatgatt attcatcttg ttgcttttta atattttttt gtagctgtgc
     3121 aaaaaaatca ttcgttccct cagtacttaa ctgctcactc cctgtgctac tagctatctt
     3181 ctcaatttga tgtagaagat taatatgccc tggcgcaaca ccaagaacca accaagcatt
     3241 tgcaatttca acgatgacga tccgctctcg ttgcccaaca gctgcagcgg ccacaacttt
     3301 aattaaattg gattgattac caggccctat tccaaaacgt ttaagtaacc aagcgattac
     3361 tccaatgaca gccaatacca acaatagggc caagaacata tgtagcacac tgcttcccgt
     3421 tacgagcgat ggcatagcag ctatctcagg cttagcaacc tcttccgctc gaagtggtac
     3481 ttgcaaattc attatgagaa agagtagtaa gaaatgaact tttagcatta tcgattaagc
     3541 ttccgaattc gttcgcttgg agtaatgata tcggttaagc ggataccaaa tttttcattg
     3601 accacgacga tctcaccctg agcaattagg caaccattca ccaaaacatc cattggctca
     3661 cctgccattc catctaactc aacgattgag ccttgcgcta gttgtaataa atttcggatt
     3721 gcaattcttg ttcgcccaag ctcaacagtt agttgaactg gtatatcaag aataatatca
     3781 atatcattct tggcgttatt taacacattg tcttttgaaa actgttcaaa aacgggtgtt
     3841 gatgctgtta gcgtgtcgct agatattgat tgatggttac tctcaggaat cgctgcaggg
     3901 tctgatgctg tagatgactg ctcttccata gcttgtgccc aatcatcagc ttcaattggc
     3961 ttttctgttt gttctgtttc actcatagct tactccgtag aattttcaga atgggaaaga
     4021 atcgatttaa ctttcaatgc atattgccca ttaagaacac catattgaca ttccaataat
     4081 ggcacaccat cagcaagagc agtaacagtg tcgggaatat ccaatgcaac aacatctcct
     4141 tcttgcatat ttagaatctg atttagggta atcttcgcat gcccaatatt tgcgattagc
     4201 tccacttcag cactttgtac ctgcttagtt aatgacctga cccaccgttt atcaagctct
     4261 aattgatcac cctgtaaagt actactcagt gtgtcgcgaa tgggttcaag catggtgtaa
     4321 ggaatgcaag tatgaaattc acctcctgtt ccaccgatgt caaccgtaaa tgtggttgcg
     4381 ataacaattt cacccggggt agcaatgctt gcaaactggg gattcatttc tgtgcgtact
     4441 tgttcaacat caatgggata tactgatttc caagattttt ggtagtcttc gtaaacgact
     4501 tcgagcaatc gagaaataat gcgttgttca gttagcgtaa actccctacc ctcaactcgc
     4561 caatgaaaac gaccatctcc accgaataaa ttatcgataa tcaagaaaat gagcttggga
     4621 tcaaaaacaa ttagagccgt acctcgcagt ggcttcatac taaccaaatt catattggtc
     4681 ggtaccacta acgatcttat aaattcactg tatttgaccg ttttcatcgt acctggcgag
     4741 atatcggctg cacggtgcat gaaattgtaa agcccgatct gcaagaatct agcaaatcgc
     4801 gcattaataa tttcgtaagt tggcatgcga ccgcgaacaa tccgttctt
//
LOCUS       DAOQLJ010000159         4809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_8312_length_4809_cov_7.481069, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000159 DAOQLJ010000000
VERSION     DAOQLJ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8312_length_4809_cov_7.481069"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PK522_09830"
     CDS             complement(<1..424)
                     /locus_tag="PK522_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNC42070.1"
                     /translation="MEIISDISLLRTRLKPEQSIGFIPTMGNLHKGHLSLVTITQQHA
                     RCTVVSLFVNRLQFSPHEDFDRYPRTLQNDFALLKEAGVDIVFLPDEKTIYPAPQTFQ
                     LLLPALADTLEGAFRPGFFRGVTTVVLKLFNIVQPHVAV"
     gene            complement(470..1261)
                     /gene="panB"
                     /locus_tag="PK522_09835"
     CDS             complement(470..1261)
                     /gene="panB"
                     /locus_tag="PK522_09835"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNC42071.1"
                     /translation="MRTTRDTLQRMYAEGKKIAVLTCYDATFAHLLENVGIDILLIGD
                     SLGSVLQGHDHTLSVTLDDMIYHTQCVVRGSNKAFIMVDMPFGSSQVSPEETFVNAAR
                     LVSVGAQMVKIEGGQIMAETVDFLTRRGIPVCGHIGLMPQSVHQLGGYKVQGRTTNEA
                     EQLFNDAMVLQEAGAAMVVLETIPASLASKITRELSIPTIGIGAGIDCSAQVLVLHDM
                     LGIYPGNKPRFVKNFMSEASSIQAAIERYVEEVKTGQYPGAEHSF"
     gene            complement(1274..1921)
                     /locus_tag="PK522_09840"
     CDS             complement(1274..1921)
                     /locus_tag="PK522_09840"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNC42072.1"
                     /translation="MLLGKYRYIVIEGPIGVGKSSLAQNMANYFNCATLLEKPTENPF
                     LENFYHDIARYALPTQLTFLLQRTDQFKQLIETNLSNKPLIGDFLLEKDQLFAKLTLS
                     SSEYAIYQKIYQHIQPQAPNPDLIIYLQASPEILIERVKRRNHAFEKTITEDYLWKLT
                     NSYTQFFHQYKKAPILIVNSENLNFANNTKDFNLLLQQISQMRGAIEYFSCSEIH"
     gene            complement(1924..2448)
                     /gene="folK"
                     /locus_tag="PK522_09845"
     CDS             complement(1924..2448)
                     /gene="folK"
                     /locus_tag="PK522_09845"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNC42073.1"
                     /translation="MNNSELLPSLEKNKLDHIAFIGLGSNLENPVLQINRALAHLARL
                     SEICILKQSSLYSSAPIDAPNQPDYINAVVKVKTSLQPQALLEHLLAIEENSGRIRTY
                     PNAPRILDLDLLLYDDLQYCEKNLIVPHPRMHQRAFVLRPLVEIENDSVIPGIGLASE
                     LLNQCNQQKLERLT"
     gene            complement(2467..3849)
                     /gene="pcnB"
                     /locus_tag="PK522_09850"
     CDS             complement(2467..3849)
                     /gene="pcnB"
                     /locus_tag="PK522_09850"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HNC42074.1"
                     /translation="MIRKFIKRFFTRAVTPSSQDVLPPTLCRISRKQHGITRNQISAS
                     ALKVTLALQQAGYSAYIVGGAIRDLLLDIKPKDFDIATNAMPEEVRGLFRRSRIIGRR
                     FRLVHVMFGDEIVEVSTFRANYSADNDAGMMAPNPADPHGRLLHDNIFGSQEEDVLRR
                     DFTMNALLYNPATEEILDYLNGYQDIQARCLRIIGNPEQRYREDPVRMLRAVRLTAKL
                     NVQLDEITAAPIGDLAPLLQNVPSARLFDEILKLLLSGHSLACLIDLRKRGLHHGLLP
                     ILDIVLEQPLGERFISIALKNTDERIQQNKPVSAGFMFAILLWHETLAAWNRRLALDE
                     KPIPALHQAMTETLSTQRAQIAMPRRFDSMIYDIWTLQPRFQSRSGTRPFRLLEHPRF
                     RAAYDFLWLRCESGEIDKEIAEWWHAFQHASPANRESMLIKETPVKKRKRNTRKRKPI
                     VSAESEKNDQ"
     gene            complement(3911..4657)
                     /locus_tag="PK522_09855"
     CDS             complement(3911..4657)
                     /locus_tag="PK522_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC42075.1"
                     /translation="MLSVHNLNLQQSERVLCGPLNFTIKPGEFWIILGENGCGKSTLL
                     QTMSRLRMENSGTITLQEKPLEKIHQPMLAQQIGILLQEETPEFWGKVREYILLGRHP
                     YKKSLSGWNHTDHTIVDQILKTHALESLANRFFNTLSGGERQRVRIAQLLAQSPQYFL
                     LDEPLQHLDIRHQLHIMTLFKNISEQNCAVVMVLHDINWLNHFGDHILMMFSDGNALS
                     GPKEMMLTQLNLDKLYQCPIAFFKHYINIP"
BASE COUNT         1320 a          935 c         1094 g         1460 t
ORIGIN      
        1 atacggcaac atggggttga acaatgttga ataacttgag tacgacagtc gtaacccctc
       61 taaaaaaacc aggacggaac gccccttcta aggtatcggc cagcgcggga agtagcagtt
      121 gaaatgtttg gggtgcgggg tagatcgttt tttcatcggg tagaaaaaca atatcaaccc
      181 ctgcttcttt tagtagcgca aaatcattct gtaaggtacg gggatatcga tcaaaatctt
      241 cgtgaggcga gaattgtaag cgatttacaa ataaactgac tactgtacag cgtgcatgct
      301 gttgtgtaat ggtgacgagt gataagtgtc ctttgtgtaa attccccatg gtaggaatga
      361 atccaatcga ttgctcaggc ttaagtcttg ttctgagtag cgaaatatcg gaaattattt
      421 ccatcaggat tggcgatcaa agaaatgcga gcaaatagcg ctttagtttt tagaagctat
      481 gctcagcacc tggatattgg ccagttttca cttcttcaac gtagcgttca atggccgctt
      541 gtatactgct tgcttcactc ataaaatttt taacgaagcg aggtttgttt ccggggtaga
      601 ttccaagcat atcgtgtagg acgagtactt gggccgagca atcgatgcct gcaccgatcc
      661 caatggtagg aatcgatagt tcacgtgtga ttttactagc aagcgaagcc gggatcgttt
      721 ctaacactac cattgctgct ccggcttctt gcaataccat cgcatcattg aataattgct
      781 ctgcttcatt tgttgttcta ccttgaactt tatagccacc gagctggtgg acactttgtg
      841 gcatcaaacc gatatgccca caaacgggaa tgccacgacg agtcagaaaa tcaacggttt
      901 cagccataat ttgcccacct tcaattttca ccatttgcgc accaaccgat actaaacgag
      961 ccgcgttgac gaaagtttct tcaggagaga cttgcgaaga gccgaagggc atatcaacca
     1021 tgatgaatgc tttatttgag ccgcgcacga cgcattgggt atggtaaatc atatcatcca
     1081 aggtgaccga taatgtatgg tcatgtcctt gtaaaacact gccgagtgag tccccaatca
     1141 gcaaaatatc gatacctaca ttctcaagta agtgggcaaa ggtcgcatca tagcaagtga
     1201 gcaccgctat ttttttacct tccgcgtaca ttctttgtaa tgtgtctcgt gttgttctca
     1261 tatgttcttc taattaatgg atttcgctac aactaaaata ttctattgca ccacgcattt
     1321 gactaatttg ctgaagtagt aaattaaaat ccttggtgtt gttagcaaaa ttgagatttt
     1381 cactattcac aatcaaaata ggggcttttt tatattgatg gaagaattgt gtataactat
     1441 tggtcaattt ccaaagataa tcttctgtga tggttttctc aaatgcatga tttcttcgct
     1501 tgacccgttc aatgagtatt tcaggcgagg cctgtaaata gatgattaaa tcgggattgg
     1561 gtgcttgtgg ctgtatatgc tggtaaattt tttgatagat cgcatattct gagctggata
     1621 acgtgagttt ggcaaatagc tgatcctttt ccagtaaaaa atcaccaatt aacggtttat
     1681 tagatagatt ggtttctatt aattgcttga attgatcagt tcgctgcagt agaaaggtta
     1741 attgagtggg taatgcgtaa cgagcaatat cgtggtagaa attttcgaga aaagggtttt
     1801 cggtcggttt ctctaatagg gtggcacagt tgaaatagtt agccatgttt tgagcaaggc
     1861 tggacttgcc aacaccgata ggcccttcaa tgacgatata gcggtattta cctaacagca
     1921 tgattatgtg agtcgttcca gtttttgttg attacattga tttaataatt ctgaagctaa
     1981 cccaatccca ggaataacgc tatcgttttc gatttctacg agtggtcgaa gtacaaaagc
     2041 gcgttgatgc attctggggt gggggactat caaatttttt tcacaatatt gcaaatcatc
     2101 atataacaat agatctagat ccagtatacg cggtgcgttt ggataagtgc gtatacgccc
     2161 gctgttttct tcaatcgcta agaggtgttc gagtagtgcc tgcggttgaa gtgatgtttt
     2221 aacttttacc acagcattaa tataatccgg ttgattaggg gcatcgatcg gggcgctgct
     2281 gtaaagtgac gattgtttta gaatgcagat ctctgaaagt ctggctagat gagcaagtgc
     2341 ccgattaatt tgcaaaacag gattttcaag attactgccc aatccaatga aggcgatatg
     2401 atctagtttg tttttctcca aagaagggag aagctctgaa ttgttcatcg ttatgtgcaa
     2461 ttggatctac tgatcgttct tctcagattc tgcagatacg atgggttttc gttttcgggt
     2521 gttgcgtttg cgttttttaa caggcgtttc tttgataagc atcgactctc tattggccgg
     2581 gctggcgtgt tggaatgcat gccaccattc ggcgatttct ttatcaatct caccactttc
     2641 acagcgtaac cataagaaat cataggctgc tcgaaaccgt ggatgctcta atagacggaa
     2701 tggcctggtt cctgagcgtg actggaagcg cggttgcaat gtccagatat cgtatatcat
     2761 ggaatcaaac cgtctcggca ttgcgatctg ggcgcgttgt gtcgatagcg tttcggtcat
     2821 ggcttgatga agtgccggta tcggtttttc atccagtgct aatcgtcgat tccaagcagc
     2881 gagtgtttca tgccagagta ggatcgcaaa catgaatcca gcggaaactg gcttgttttg
     2941 ttggattctt tcatccgtat ttttgagggc tatcgaaata aagcgctcgc caagcggttg
     3001 ttctaggacg atgtcgagta tgggcagtaa accatggtgt aggccacgtt tgcgcagatc
     3061 gatcaggcat gccaaagaat ggccagataa taatagtttt aatatttcat cgaataaacg
     3121 tgcagaagga acattttgta ggaggggagc taagtcacca attggcgctg cagttatttc
     3181 atcgagctga acgttaagct tggcggttaa tcgaacggca cgtagcatgc gtaccggatc
     3241 ttctcggtaa cgttgctcag gattaccaat gattcgtaaa caccttgctt gaatgtcttg
     3301 atagccattc aaataatcaa gaatttcttc tgtggccgga ttatagagca gcgcgttcat
     3361 cgtgaagtca cggcgcagca catcttcttc ttggctaccg aaaatattat catggagcaa
     3421 gcgcccatgg gggtcagcag ggttgggtgc catcatgcct gcatcgttat cagcactata
     3481 atttgcacga aatgtcgata cttcaacgat ttcatcgcca aacatcacat ggaccaggcg
     3541 aaatcgtctt ccaatgattc gcgatctgcg aaataatcct ctgacttctt cgggcattgc
     3601 attcgttgca atatcgaaat ctttaggctt aatatcaagt aacagatcac ggatagcccc
     3661 accgacgatg taggctgaat atccagcttg ctggagtgca agggtaactt ttaaggcact
     3721 ggcactgatc tgattccgtg ttatgccatg ctgtttacgt gaaattctac aaagtgttgg
     3781 aggtaggaca tcctggctcg atggagtaac tgcccgagtg aagaagcgct taataaattt
     3841 acgtatcatt acaagatgct aaaagtatca ttggatatta tacactgagc agcgtgtcag
     3901 taacgaagac ttaagggatg ttaatgtaat gcttgaaaaa agcgataggg cattgataaa
     3961 gcttatctaa attcaactgg gtcaacatca tttctttagg tccgcttagt gcattgccat
     4021 cgctaaacat catcaagatg tgatccccga agtggtttag ccaattgata tcatgcagaa
     4081 ccatcaccac tgcacaattt tgttcggaaa tgtttttaaa taaggtcatg atatggagtt
     4141 gatgtcgtat atcgaggtgc tgcagcggtt catctaacaa aaaatattga ggggattgtg
     4201 ctaacaactg agcaatacga acacgttgac gttccccgcc tgaaagggta ttgaaaaatc
     4261 gattggcaag cgactctaat gcgtgagttt taaggatttg atcgactatc gtatgatccg
     4321 tatgattcca tccggataac gactttttgt aagggtgacg acccagtaga atatactctc
     4381 taacctttcc ccaaaattct ggcgtttctt cttgtagcaa aattccaatt tgttgcgcaa
     4441 gcatcggctg atgaattttt tctagcggtt tttcttgtaa cgtgatagtt ccgctatttt
     4501 ccatccgaag tcgactcatc gtctgtaata gtgtagattt tccgcaacca ttctctccca
     4561 agatgatcca aaattctcca ggtttgatcg taaaattaag tggcccgcag agtactcttt
     4621 ccgattgctg gagattgagg ttatgaacag atagcattgg gtattagtaa tggttaatgc
     4681 taaagaatag cttgtatgag gaaaaatagt gaatacttga agttcaattc agttgtcaga
     4741 aatattttta attttattca ttaataatta tccaaatgga taattattaa ttgaaataat
     4801 tccttatta
//
LOCUS       DAOQLJ010000160         4777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_8384_length_4777_cov_6.242058, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000160 DAOQLJ010000000
VERSION     DAOQLJ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8384_length_4777_cov_6.242058"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="PK522_09860"
     CDS             complement(<1..1016)
                     /locus_tag="PK522_09860"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HNC42076.1"
                     /translation="MKRYLKPAVLVIFSVFFGFNLLHTHAKDDFSEIVLVDGKVQSRV
                     ELDQGWSQEIRQLFWFTSQGSRIIPYNWFAWLEQADNNSLFRNVEHMEMLRYLPMNSS
                     KLNPAGLPIGFALDTDHSTGRKWVGLTCAACHTNQIDYQGTRMLIEGAPTLANFVKFF
                     DDLVNALNKTNDNPQKFTRFAKNVLGNQYSETTANKLHQELDQIAAKLAQRRMVNQLP
                     PDYPEDFTSYARLDAFGNIQNAASAFALHDLTNRNAPTAPASYPFLWGTHQSDVVQWN
                     GSAPNTSIIGPLIRNMGEVVGVFGDLEISEAPIWKKLLGIKVEYNAHVDIHNLGKLEL
                     WVKH"
     gene            complement(1215..2570)
                     /gene="mnmE"
                     /locus_tag="PK522_09865"
     CDS             complement(1215..2570)
                     /gene="mnmE"
                     /locus_tag="PK522_09865"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNC42077.1"
                     /translation="MYSSDIIAAIATPPGRGGIGIIRISGNNLKEITQQLLGDLPKPR
                     YASLNYFLDQNHEVIDHGIAIYYVAPHSYTGEDVLELHGHGGPAVINLLLKRCLALGA
                     RLAEPGEFTLRAYLNNKIDLVQAESIADLIDASTAGAARSALRSLQGKFSEAIHELVR
                     ALIDLRIQVEAALDFPEEDIDWLETTHLKTQLNQLYEKLEKVYVSSQRGNLLREGAKI
                     VLIGQPNVGKSSLLNRLAGDEVAIVTEIPGTTRDAIQQLIQIDGVPLHIIDTAGLRET
                     EDIVEKKGIERTYTAMEQADLALLLIDSQNELSEADQNLLSKLPVNLPVLIVFNKIDL
                     LNQPAKSEDKNGYSMIYLSAKTGVGIEILCEKILTLVGWRSHVAGEGIFMARQRHLQA
                     LDQAQRHLSNAIAWASSGKAIEVLAEELRLAQYSLSSIVGEFSADDLLGEIFSHFCIG
                     K"
     gene            complement(2694..4421)
                     /gene="yidC"
                     /locus_tag="PK522_09870"
     CDS             complement(2694..4421)
                     /gene="yidC"
                     /locus_tag="PK522_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNC42078.1"
                     /translation="METKKLVLIFIFITSLLFIWEGWMRERERLDNLANGTLVTDNNS
                     VPQQGPDSPPIPGDALVSSQSNQDRASGIDAASASTGTPRNLPTGEQIKVITDMVIAE
                     IDTAGGDVRKLELLKHPSRENKEIPYALLHSDATQTSVAQSGLIGEGFPTHKTQYTLE
                     TKVREYSLEADQDKIEVRLVAPRENEVQVTKIYTFNRGSYVIDVRFEIDNQSEEIVRP
                     FAYFQMLRDSNPPAGATMMIPTYLGAAIYTEDEKFQKIQFSDLDKNKADYPRNADNGW
                     IAMLEHYFLSAWLPPQKAPREYFAKRQNDNLYTAGVISPVGAIDPGKTTSISMPLYAG
                     PQEQEKLAELAPGLELTVDYSWLTVIAAPLFWLLSFYHEWVGNWGVAIILLTMTVKLL
                     FFPLSAAGYRSMAKLRLVTPKLQRIQEQYKSDRQQMHQAMMELYKEEKINPLGGCLPI
                     LVQIPVFIALFWVILAAVELRYAPFALWITDLSSPDPLYVMPLLMGISMFIQFKLNPK
                     PTDPIQAKVLQIMPIAFSIFFFFFPAGLVLYSLVNNILSILQQWQITRLYGQPSSNDA
                     STKKKVKEK"
     gene            complement(4511..4720)
                     /gene="yidD"
                     /locus_tag="PK522_09875"
     CDS             complement(4511..4720)
                     /gene="yidD"
                     /locus_tag="PK522_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNC42079.1"
                     /translation="MRQLLIKIIALYQYCISPLLPPSCRYSPTCSMYAKEALLKYGVM
                     KGIVLSIKRILRCHPWSRGGYDPVP"
BASE COUNT         1402 a         1048 c          921 g         1406 t
ORIGIN      
        1 aaatgcttaa cccatagctc cagcttaccc aagttatgga tatcgacatg cgcgttatac
       61 tccactttga ttccaagtaa ttttttccag attggtgctt cactaatctc caaatctcca
      121 aaaacaccaa ctacctcacc catattacga ataagcggtc caatgatcga tgtattgggc
      181 gcagacccat tccattgaac aacatctgat tgatgcgttc cccacaaaaa agggtaggaa
      241 gctggtgcgg ttggtgcatt tctattcgtt aggtcatgca aagcaaaagc agatgcagca
      301 ttttgtatat tgccaaacgc atctaaacgc gcatagctcg taaaatcctc aggataatcc
      361 ggtggtaatt gattaaccat ccgacgctgc gctaatttag ctgcgatctg gtcaagctct
      421 tgatggagct tatttgcagt tgtttctgag tattgattgc ccaatacatt tttagcaaat
      481 cgggtaaatt tttgtggatt atcatttgtt ttatttagcg cattaaccaa atcatcaaaa
      541 aatttaacga aattagcaag cgttggtgcg ccctcgatta acattctcgt tccttgataa
      601 tcgatttgat tggtatggca agcagcacaa gtaaggccta cccattttct tcctgtgctg
      661 tgatcagtat ccagagcgaa tccaatgggc aatccagcag ggtttagctt cgaggaattc
      721 atgggaagat agcgcagcat ctccatatgt tcaacattgc gaaataagct gttgttatct
      781 gcctgttcta accaagcaaa ccaattgtag ggaataatgc gagaaccttg gctggtaaac
      841 caaaataatt ggcggatttc ttggctccag ccttgatcta gctctacgcg agattgaact
      901 tttccatcta caagaacaat ttcagaaaaa tcatctttcg catgagtgtg aagcaagttg
      961 aatccaaaga atactgaaaa tattacaagt acagcaggtt taaggtacct tttcatgatt
     1021 aatcccccta tatttcagtt gcgtgacaat taaaataacc gcggtccatc agttcaagcc
     1081 tctagctgaa gcactcgatc atcccatttt tccctgggga ttaatatagc aggtggataa
     1141 ttattcttat gtgagaaaaa ccatattaaa gttattaaaa ttgaacaaat ttcggttttc
     1201 tagtggtctt tttactattt accaatacaa aaatgcgaaa aaatttcgcc caagagatca
     1261 tctgcactga attccccgac gattgatgat aaggaatatt gcgcaagccg taattcttca
     1321 gccaggacct ctatcgcttt tccactgctt gcccaggcga ttgcgttact taaatgtctt
     1381 tgcgcttgat caagcgcttg taagtgtcgc tggcgagcca taaatatgcc ttcgccagcg
     1441 acatgtgaac gccaaccgac taatgtaagg atcttttcac ataatatctc gataccaaca
     1501 cctgttttag ctgaaagata aatcattgag tacccatttt tatcttcact cttcgctggt
     1561 tgattcagca aatcaatctt attaaaaacg ataaggacag gaagatttac aggtagtttg
     1621 ctcaataaat tttgatcggc ttcagataac tcattttggc tatcgatcaa cagcaaggct
     1681 aaatccgctt gttccatcgc agtataggtg cgttcaattc ctttcttttc cacaatatct
     1741 tccgtttctc ttaatcctgc ggtatcgatt atgtgtaatg gaacgccatc aatctggata
     1801 agctgttgaa tcgcatcgcg ggtggtgcct ggaatttcag taacgatagc cacttcatca
     1861 ccggccagtc ggtttaataa gctcgatttt ccgacattgg gttgtccaat caatacaatt
     1921 tttgcgcctt ctctcaacaa attgccacgt tgtgatgaaa catagacttt ctcaagtttt
     1981 tcgtataatt gatttaactg tgtttttaag tgcgtggttt cgagccaatc gatatcctct
     2041 tcaggaaaat ccagtgccgc ttcgacctgt atgcgcaaat cgatcaatgc gcgaactaat
     2101 tcgtgaatcg cttcagaaaa ttttccttgc aaagaacgca gtgcgctgcg tgctgcacca
     2161 gccgtgctag catcgattaa atccgctatg ctctcagctt gcactagatc aattttatta
     2221 tttaagtagg cacgcaatgt aaactcacca ggctcggcca agcgagcacc taatgctaag
     2281 catcgcttca ataataaatt gatgacagca ggacccccat gtccatgaag ttctaaaaca
     2341 tcttcgcctg tgtaagaatg gggagcaaca taatagatgg caattccatg atcgattact
     2401 tcatgatttt gatcaagaaa ataattcaaa cttgcgtagc gcggcttggg tagatcccct
     2461 aacaattgtt gggtgatctc tttgagattg ttaccagaaa tacggataat tcctatccca
     2521 ccacgtcctg gtggtgttgc gatagcagca atgatgtcac tgctgtacat gaagcattta
     2581 ctctaatcgt tagtatgtct aattaatgaa aaacaccaaa aaatattatt accggaattt
     2641 caaccgacga atgagcatta cttaataaaa tactcattcg ccgatactct ctattacttc
     2701 tcctttactt tctttttcgt tgatgcgtca ttacttgatg gttgaccgta caaacgcgta
     2761 atttgccatt gttgcaagat agataaaata ttgtttacca aagaatacaa tacaagccct
     2821 gcgggaaaga aaaagaaaaa tatgctaaag gcaattggca tgatttgcag aaccttagcc
     2881 tggatcgggt cagtaggctt aggatttagc ttaaactgga taaacatcga tatccccatt
     2941 aataagggca tgacgtaaag cggatcgggt gatgataaat cagtaatcca taatgcaaac
     3001 ggagcatatc gtaactcgac agccgcaaga atcacccaga aaagtgcgat gaatacaggt
     3061 atttgcacca aaataggtaa acatccacct aatgggttaa ttttttcttc tttgtaaagt
     3121 tccatcatcg cctgatgcat ctgttgacga tcactcttgt attgttcctg tatgcgctgc
     3181 aacttaggcg ttacaagacg caatttagcc attgaacgat agccagcggc tgataatggg
     3241 aaaaataaaa gcttgactgt catagtcagt aagatgattg caacacccca attaccaacc
     3301 cactcatgat aaaacgatag tagccagaac aatggcgccg cgatcacagt taaccagctg
     3361 tagtcaacag tcaattcgag gccaggcgcc aattctgcta gtttctcttg ctcctgcggc
     3421 cctgcataaa gcggcatcga aatactcgtt gtttttcctg gatcaatcgc acctactggg
     3481 gaaataactc ctgcagtata taaattatca ttttgacgct ttgcaaaata ttcgcgaggc
     3541 gctttttgtg gaggaagcca tgcagaaaga aaatagtgct ccagcattgc aatccagcca
     3601 ttatccgcat tccgaggata gtctgcctta ttcttatcta agtctgaaaa ctggattttc
     3661 tgaaatttct catcttcagt ataaatcgca gcaccgagat aagttggaat catcatcgtt
     3721 gcaccagccg gcggattact gtcccgcaac atttgaaaat acgcaaatgg gcgaacaatt
     3781 tcctcgcttt gattatctat ttcaaatcga acatcaatga catagcttcc acgattgaat
     3841 gtataaatct tagtgacttg aacctcattt tcccgtggag caaccagccg aacttcaatt
     3901 ttatcctgat cagcttcgag actatattca cgtactttag tctctaaggt atattgcgtt
     3961 ttgtgggtag gaaatccttc accgattagc ccagattgcg ctacgctggt ctgcgtagca
     4021 tcactatgca aaagcgcata aggtatttct ttattttccc ttgagggatg tttaagtaat
     4081 tctaatttac gcacatcccc accagccgta tcaatctcag cgattaccat atcagtaata
     4141 actttgattt gctccccagt tggtaagtta cgtggtgtcc cagtggatgc tgaagcggca
     4201 tcaatccctg atgcgcgatc ctgatttgac tgagaagaga cgagcgcgtc accgggtatt
     4261 ggaggtgaat cagggccttg ttggggaacg gaattattgt cagttaccag ggtaccgtta
     4321 gcaagattat ctaatcgctc ccgttccctc atccagcctt cccaaataaa cagcaatgaa
     4381 gtgataaaaa taaaaatcaa tacaagcttc ttcgtttcca tcttaaatta aacgtgttat
     4441 ttttttacga taaaccttcc cataacagga agtcgatcat tattattaaa attttattac
     4501 ctttcaggat tcagggaaca ggatcataac caccccgact ccagggatgg caacgcaaaa
     4561 tgcgcttaat acttaatact attcccttca taacgccata ttttagcagc gcttcctttg
     4621 catacattga acatgtcgga ctatagcggc aagaaggcgg taataaagga ctaatacaat
     4681 actgataaag ggcaatgatt ttgattaaca attgtcgcat tgccttgact tcaatatcag
     4741 tatctcagct tcttttgcga taccagcagg aacaatc
//
LOCUS       DAOQLJ010000161         4729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_8520_length_4729_cov_5.658323, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000161 DAOQLJ010000000
VERSION     DAOQLJ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8520_length_4729_cov_5.658323"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            370..1269
                     /locus_tag="PK522_09880"
     CDS             370..1269
                     /locus_tag="PK522_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42080.1"
                     /translation="MSIPTETYEAVFEQYSAAMRWMTELGIKLNPGRTSHYEKIVGHW
                     KDAYKTATTDERRQTFPDFISSMFEVFDFVSIYKAFESVPVHQLTSIVEKLQKGVNGP
                     INAADERPDSTVARNFLFEVAVAAKAYRPDKGIETILDARSDTGISLSGKKLWVECKR
                     VTTIDKIESNVRKASSQLEAVLAREIGSGHRGIVALDVSKILNRGDKIFVTRNDDDLS
                     ASVDRIMDQFIEKYSHIWQLVYQRREKKIIGTIIRFSFMASSEARELLVYASQWAMNP
                     RLSVLESDEQIQRQLVATLANAP"
     gene            complement(1395..2849)
                     /gene="rng"
                     /locus_tag="PK522_09885"
     CDS             complement(1395..2849)
                     /gene="rng"
                     /locus_tag="PK522_09885"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HNC42081.1"
                     /translation="MNSEILINMTPQETRVAVIEQGVVQELHIERTSSRGIVGNIYNG
                     RVSRVLPGMQSAFIDIGLERAAFLHVADIKEFGSNTDADKPIERLLFEGTSILVQVIK
                     DAIGTKGARLSTQISIAGRMLVYLPYESHIGISQRIEDNAERESLREKLQRVLPPEDK
                     GGYIIRTMAEAASESDLRADIEYLHKLWHDIQNKSLSASPPALLYQDLDLSHRVLRDF
                     LDESITGIIVDSRETHQKLKAFAQNFMSNVTDKIDHYTGERPLFDLYGVEEEIEKSLS
                     RRVNLKSGGYVIIDQTEALTTMDVNTGGFIGVRNFDDTIFKTNLEAAQVIARQLRLRN
                     LGGIIIIDFIDMENQEHRNAVLVEFNKALMKDRTRMTVNGFTALGLVEMTRKRTRESL
                     AHILCETCPTCQGRGEIRTAQTICYEILRELLRESRQFNANEYRILASQPVIDLFLDE
                     ESQSLAQLGDFIAKPISLQVEASYTQEQYDVILI"
     gene            complement(2881..3504)
                     /locus_tag="PK522_09890"
     CDS             complement(2881..3504)
                     /locus_tag="PK522_09890"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNC42082.1"
                     /translation="MAYTENQIYLASRSPRRRELLKQIGIKHSILLMRETMDRSVDID
                     ETPDAEESPADYVYRMVHTKADASWKRLTQRKLPILPILVADTIVALDGCILGKPRDA
                     AHAEEMLLSLSGRTHQVLTAIALSMQGDVKHRLSITTVRFREIGKREIQACIKGGETH
                     DKAGAYAIQGLAAAFIVEIYGSYSGVVGLPLFETAQLLEESGVALFS"
     gene            complement(3575..4036)
                     /gene="rlmH"
                     /locus_tag="PK522_09895"
     CDS             complement(3575..4036)
                     /gene="rlmH"
                     /locus_tag="PK522_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNC42083.1"
                     /translation="MKFNVLAVGNKMPDWIESGFMEYRKRMPKELSIELIEVKQVKDK
                     NREQSLLAEYERIAKVLPTANYIVAMDEQGKQLSTQELAASLINWMNLGKDITFIIGG
                     ADGLHPVIKEKADQVLSLSKMTLPHGLVRVLLAEQLYRATTILKHHPYHRA"
     gene            complement(4038..4388)
                     /gene="rsfS"
                     /locus_tag="PK522_09900"
     CDS             complement(4038..4388)
                     /gene="rsfS"
                     /locus_tag="PK522_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNC42084.1"
                     /translation="MNTNQLIKITIEALEDIKANEIVVLEAEKNVSLFDYIVVASATS
                     SRQANAIANHIREKVKAAGGAIYGIEGERTGEWLLVDLGDVIVHIMQPTTRAYYNLEA
                     LWSESARYQTHPQP"
     gene            complement(4385..>4729)
                     /locus_tag="PK522_09905"
     CDS             complement(4385..>4729)
                     /locus_tag="PK522_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNC42085.1"
                     /translation="GADAFMKLPSWYHWRELFDLCHLIVVNRPGFSLINQQSDLPHEL
                     TIECQDRWVEQADLLKQRASGLIYCAATTPVAISATTIRNAILTGKSIRYLLPDSVIN
                     YIYRYHVYPGEK"
BASE COUNT         1274 a         1083 c         1017 g         1355 t
ORIGIN      
        1 atttgatctg tgcgttaacg cacagtaata gatctagcgt tggagttccc cattttatag
       61 ggcagcctta tcaaacctgg ccgtgaaagg tagcgcaact agcaaacact tcattcaagc
      121 aatatcaaat tgctcagtac gaaactgtaa tacttaaacc tgagttttcc ccgtggctaa
      181 tttagaagtt ggcgtttgtt ttctgtttaa tgattcatgt aatttgtaag cacaaaattt
      241 ggtatgatga taactaattc gttcaatcgt tatagtcatg aggaaattcc atgcctaatt
      301 ccgtattaga tctcatttgt gacgtaataa atcgcgtcat ttcggacatc taattctagt
      361 taggttcgca tgtccatccc caccgagact tacgaagccg tctttgagca gtattcggcc
      421 gcgatgaggt ggatgactga acttggtatc aaactgaatc caggaagaac ctctcactac
      481 gaaaagatcg ttggacattg gaaggatgct tacaagactg caactacgga tgaaaggcga
      541 cagacattcc cagatttcat cagttcaatg ttcgaagtat ttgactttgt gagcatctac
      601 aaggcgtttg agagcgtccc agtccatcag ctcacatcta tcgttgagaa gcttcaaaaa
      661 ggtgtgaatg ggccgatcaa cgccgcagac gaaaggccag actccaccgt tgctcgcaat
      721 ttccttttcg aggttgcagt cgcagcaaag gcttatcgcc ctgataaagg tattgaaacc
      781 attctcgatg cgcggtccga tactggcatc agtctgagtg gaaagaagtt atgggtcgag
      841 tgcaaacgcg taacaaccat cgacaagatc gagagtaatg ttcgaaaggc ttccagtcag
      901 ctagaagcgg tacttgcgag ggagataggt tctggccacc gtggaattgt tgccttggat
      961 gtttccaaga ttttgaatcg cggtgacaag atttttgtga cgaggaacga cgatgatctg
     1021 tcggcgtccg tggatcgaat aatggatcag ttcattgaaa agtactctca tatctggcaa
     1081 ctcgtttacc agagaaggga gaagaaaatc atcggaacga tcattagatt ctcgttcatg
     1141 gccagttcgg aagccagaga gctcttggtg tatgcgtcgc aatgggctat gaacccgcgc
     1201 ctgagcgtcc ttgagagtga cgagcaaatt caacggcagc tcgttgccac gctcgcaaac
     1261 gcgccgtaaa atgcgaactt acccccccgg ttgcaggcga cgcgcaagaa gctgcgctgt
     1321 aataacctac cccttcatga gtggtgtgat tataaaatca cagacaacct ttctagccgc
     1381 tcttacattc attcctaaat caagatgaca tcatattgct cttgtgtata ggatgcttct
     1441 acctggaggc tgatcggttt tgcgataaaa tcgcctaact gcgctaggct ttgcgattct
     1501 tcgtccagaa ataaatcgat cacgggttgt gaagcaagga ttctgtattc attggcgtta
     1561 aactgccgcg attctctgag caattcccgc aaaatttcat aacagatggt ttgtgccgtt
     1621 cggatctcac ctcgaccttg gcaggtagga caggtttcac ataggatgtg cgctagactt
     1681 tcacgcgttc gtttacgcgt catttcaacc agtccgagtg cagtaaaccc gttaaccgtc
     1741 atgcgtgtgc ggtctttcat taatgcttta ttgaattcaa cgagtacggc attccgatgt
     1801 tcctggtttt ccatatcgat gaaatcaata atgataattc cgcctaaatt tcgaaggcgc
     1861 agttgtcgcg caatcacttg agcggcttcg agattcgttt taaaaatcgt atcatcaaaa
     1921 tttcggaccc caataaaccc cccagtatta acatccatgg ttgttaaggc ttcagtctga
     1981 tcgatgatga catagccccc cgatttcaaa ttaacacggc gtgacaacga tttttcaatt
     2041 tcttcttcaa caccatataa atcgaacaag gggcgttcgc cggtgtaatg atcgatttta
     2101 tcggtgacat tgctcataaa attttgagca aatgctttta atttttgatg ggtttccctc
     2161 gaatcaacga tgatgcccgt tatcgattca tccaaaaaat ctctgagtac tcggtggctt
     2221 agatcgagat cttgatagag caatgccgga gggctcgctg ataaggattt attctgaata
     2281 tcgtgccaaa gcttatgtaa atattcgata tctgcgcgca aatcggattc actggccgct
     2341 tctgccatcg ttcggataat ataccctccc ttatcttctg ggggtaaaac ccgttgcagc
     2401 ttttctcgca aggattcacg ctctgcatta tcctcaatac gctgagagat accgatatgg
     2461 gactcatagg gtaaataaac gagcatgcga ccagcgatgc ttatttgagt tgagaggcgg
     2521 gcgcctttgg tgccaatcgc atccttgatg acttgaacta agatactggt gccttcgaac
     2581 agtaagcgtt ctatgggctt gtcagcgtcg gtatttgatc caaattcttt gatatctgca
     2641 acatgtagaa aagctgctcg ttctaggcca atatcaatga aagccgattg cattcccggt
     2701 aacacccgac tcacacgacc attatagata tttcctacga tgccacgact actggtcctc
     2761 tcgatatgta attcttggac caccccttgc tcaatgacag caacacgtgt ctcttgaggg
     2821 gtcatattga tgagaatttc actattcata ctaaagcgat cgaaacaaaa aaagtatcga
     2881 ctaagaaaaa agtgctaccc ctgactcttc aagcagctgg gctgtttcaa ataagggcaa
     2941 gccgaccacc ccactataac taccatagat ctctacgata aacgccgcag ccaacccttg
     3001 aatcgcatag gcacctgctt tgtcgtgggt ttccccccct ttgatacagg cttgaatctc
     3061 ccgtttgcca atttcgcgaa agcgcaccgt tgtaatcgat aatcgatgtt taacatcgcc
     3121 ttgcatactt aatgctatgg cagttaatac ttggtgcgtt ctacctgaga gtgataaaag
     3181 catttcttcc gcatgcgctg catctctggg tttaccgagt atgcagccat caagcgctac
     3241 aatggtatcg gcgactaaga ttggcaaaat cggtaactta cgttgtgtta atcttttcca
     3301 gctcgcatcg gccttggtat gcaccatccg gtaaacatag tctgctggag attcttctgc
     3361 atcgggtgtt tcatcaatat caacagaccg atccatcgtt tcacgcatca ataatatact
     3421 atgcttaata ccaatttgtt ttaataactc ccgccttcga gggctgcgag atgccaggta
     3481 tatctgattt tcagtatagg ccataatttt taactcagaa cggtaggcaa aagataattg
     3541 ctctctagct gtgattatcc ctgaaacacg ttatctatgc acgatgatag ggatgatgtt
     3601 tgaggatggt cgttgcacga tataattgtt ctgccaatag tactctgact aaaccatgtg
     3661 gaagggtcat tttcgaaagc gataaaactt gatctgcttt ttccttgatc actggatgca
     3721 atccatcagc accgccaatg ataaaggtaa tatccttgcc caagttcatc caatttatta
     3781 atgaagcggc aagctcttga gtcgatagct gtttgccttg ctcatccata gcaacaatat
     3841 agtttgcagt cggcaatact ttcgctatcc gctcatattc tgcaagaagc gattgctcac
     3901 gattcttatc tttgacctgc ttaacttcaa tcaattcgat tgatagttcc ttcggcatgc
     3961 gtttacgata ttccataaac cctgattcta tccagtctgg cattttattc cccacggcca
     4021 gcacattgaa cttcatgtta aggctgagga tgggtttgat agcgggctga ctcggaccat
     4081 agcgcctcta aattataata agcgcgcgtt gtaggctgca taatatggac aatcacatcg
     4141 cctaaatcga ctaagagcca ttccccggtg cgttcccctt cgataccata gattgcccca
     4201 ccggctgctt taactttttc tcgaatatgg tttgcaatcg cattggcttg tcgggatgat
     4261 gtcgcactcg caacaacgat atagtcaaac agtgaaacat ttttctcagc ttcaagcacg
     4321 acaatctcgt ttgctttgat atcctcaagt gcttcaatgg taattttgat taattgattg
     4381 gtattcattt ctctccagga tacacatgat agcgataaat atagtttatt acgctatctg
     4441 gtagtaaata gcgtatgctc tttccagtta ggatagcatt tcgaatagtt gttgcagaaa
     4501 tcgcaactgg ggtcgttgca gcgcaataga taagacctga tgcacgttgc tttaataaat
     4561 ccgcttgttc aacccaacga tcctgacatt ctatcgtgag ttcatgcgga aggtcagact
     4621 gttgattaat caaactaaaa ccgggccgat tgaccacgat caaatgacaa agatcgaata
     4681 attcacgcca atggtaccag cttggtaact tcataaaagc atcagcgcc
//
LOCUS       DAOQLJ010000162         4717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_8558_length_4717_cov_6.830545, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000162 DAOQLJ010000000
VERSION     DAOQLJ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8558_length_4717_cov_6.830545"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /gene="argJ"
                     /locus_tag="PK522_09910"
     CDS             complement(<1..1055)
                     /gene="argJ"
                     /locus_tag="PK522_09910"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNC42086.1"
                     /translation="MPVNISPLLSEQLLPIAGISLGIAEAGIKRPNRKDLLTIVLDEN
                     VNVAGVFTQNRFCAAPVIVAKDNLTQASSIRALVINTGNANAGTGEQGIADARATCQH
                     LAKLINCHVAQILPFSTGVIMEPLPVEKIVTHLPQALADLNPDHWFKAAQAIMTTDIV
                     PKGVSRQIEIQGKTVTITGIAKGSGMIHPNMATMLGYIGTDACVAKAILQQMVRHVAD
                     HSFNCITVDGDTSTNDALILMATGRAGHSEITQLESQDYLKLQTAITAVARELAQMIV
                     RDGEGATKFITVCVEKGKNREECSRVAYAIAHSPLVKTAFFASDPNLGRILAAIGNSG
                     IIDLDVDLVQLYLDDVLV"
     gene            1144..1419
                     /locus_tag="PK522_09915"
     CDS             1144..1419
                     /locus_tag="PK522_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNC42087.1"
                     /translation="MKLTKTQPLLAKRENPFRADRELDVRGLVCPLPILRTKKALSEM
                     STGQVIKIVATDPGSVIDFQVFAEQTANDLLSFTESVDEFIFYLKKR"
     gene            complement(1448..2806)
                     /gene="pmbA"
                     /locus_tag="PK522_09920"
     CDS             complement(1448..2806)
                     /gene="pmbA"
                     /locus_tag="PK522_09920"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HNC42088.1"
                     /translation="MNPDTSSGQNFSYSFAELQQVANDILNHAKRGGATACEANVSDG
                     FGLNVTVRQNAVETIEHNRDKGLSVTVYIGQKRGNASTSDFSPQAINDTVAAALSIAR
                     HTAEDNCAGLAEANLLATTFSDLDLYHPWNLPVEAAIELAQRCEAEALVVDKRITNSE
                     GASVSVGASQFVYANSLGFMGGYPVSRHSISCAVIASEADMMQRDYWFSVARDAHDLE
                     QIEAVGKKAGMRTVARLGAKKLSTCEVPVLFEAPLASSLIGHMVSAISGGSLYRKSSF
                     LLDSIGKQILPSDIQLHERPHIPKGLASCTFDEEGIATKDRSIIENGVVQGYFLNSYS
                     ARKLGLHSTGNAGGNHNLILSCAEPLGFEALLKKMNRGLLVTELLGHGVNGVTGDYSR
                     GAMGFWVENGEIGYPVEEITIAGNLKVMLSGIIGVGSDVIIRGSKQCGSILIDRMMVA
                     GL"
     gene            2938..3453
                     /gene="yjgA"
                     /locus_tag="PK522_09925"
     CDS             2938..3453
                     /gene="yjgA"
                     /locus_tag="PK522_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HNC42089.1"
                     /translation="MIHDSHDDNESEEILSKTRRKKAMHALQGIGEQLVELDSQFIAE
                     LDLPEILVDAIREAKQLTKHEAKRRQMQYIGKLMRNIDAAPIQEKIDYWKNRDVNHTA
                     WLHRLENWRTRLLADENSINEFKQHYPETDIQHLRALIRNAHKETLSQKPPKSFRAIF
                     QELRTIIPEKK"
     gene            3510..3686
                     /locus_tag="PK522_09930"
     CDS             3510..3686
                     /locus_tag="PK522_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42090.1"
                     /translation="MIDDNSRFQMAVAKAWEDWLAVLEEDSFDSKLDGQEDLIDNLTL
                     SCPQIFQKDCLTLY"
     gene            3925..4638
                     /locus_tag="PK522_09935"
     CDS             3925..4638
                     /locus_tag="PK522_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42091.1"
                     /translation="MKKVLQNNWIAKGFVVIFMVLASFSLKAQLMLAHEGHHAGHGGC
                     EVKTGSFVVTVSIYEVPEGNIPPMHSYCNHVPKPGKVNVTIELPSDAREIPLAVRLVK
                     DDSHAGHGSGSPQSNATEKAASAVNEGDDHAHHHDETHGAGEFGLHYMPPMEHHSGII
                     VVAAELTELGQYAILLEQIDDKDDVRIVSKVPVHVGGDGGHAGHGSGIGLMEIVIGLM
                     LVVGGIGAFMFRKKKPQAS"
BASE COUNT         1434 a         1000 c          986 g         1297 t
ORIGIN      
        1 accaacacat catccagata gagctgcact aaatctacat ctaaatcgat gattcctgag
       61 ttaccaattg cagccaagat acgacccaga ttagggtcag aagcgaaaaa agccgtcttc
      121 actaacggtg aatgtgcgat ggcatacgcg acccgtgaac actcttctcg gttctttcct
      181 ttttccacac aaaccgtaat gaatttggtt gcgccctcgc catcacgcac gatcatttgt
      241 gctaattctc ttgccacggc cgttatcgct gtttgaagct tcagataatc ttggctttct
      301 agttgagtaa tctctgagtg cccagctcgt cctgtcgcca ttagtatcag cgcatcattg
      361 gtagaagtat caccatcgac tgtgatacag ttgaatgagt gatccgctac atgacgaacc
      421 atctgttgca ggatagcctt ggcaacgcaa gcatcagtgc caatatagcc aagcatggtt
      481 gccatattgg ggtggatcat acccgagcct tttgcaattc cggttatagt cactgttttt
      541 ccttgaattt caatttggcg cgacacgcct tttggaacga tatcggttgt catgatcgct
      601 tgcgcagcct taaaccaatg atctgggtta aggtctgcta atgcctgcgg caaatgagtc
      661 acaatcttct cgaccggaag cggttccata attacaccgg tagagaaggg tagaatctgc
      721 gctacatgac aatttataag cttggctaga tgctgacatg tcgcacgggc atctgcgatg
      781 ccctgctctc cagtgcctgc attagcattc cctgtattga taaccaaagc acgaattgaa
      841 gaggcttggg ttaaattatc cttggctaca atcactggtg cagcacaaaa ccgattttgc
      901 gtaaaaacac ctgctacatt gacgttttca tctaacacga tggtcaataa gtctttacga
      961 tttgggcgct tgatccccgc ttcagcaatt ccaagggata ttccagcaat aggtagtaat
     1021 tgctcagaaa gtagtggcga aatattaact ggcatacgtt aaaatataaa aaaatgagtt
     1081 atcgtatctc aacacgattg agatattttg aaaatctaaa aatagatgct caataagaaa
     1141 attatgaaac taactaaaac acaacccttg ttagcaaaaa gagaaaaccc attccgtgct
     1201 gatcgcgaat tagatgtacg tggactagtc tgcccactgc ccatattacg cactaaaaaa
     1261 gccttgtcag aaatgtcaac aggacaagtc atcaagatcg tcgctacaga tcctggttct
     1321 gtaattgatt ttcaggtttt tgccgaacaa actgcaaatg atttattatc tttcactgaa
     1381 tcagtcgatg aattcatttt ttacctaaaa aagcgctaat gattggctaa tgagcgctta
     1441 aggccattta aagcccagca accatcatcc gatcaattaa tattgaccca cattgtttgg
     1501 atccgcggat aatcacatcg ctacccacgc caatgatacc cgatagcatg acttttagat
     1561 tgcccgcaat ggtaatttct tcaacggggt aacctatttc cccattctct acccaaaatc
     1621 ccattgcacc tcttgaatag tccccagtca caccatttac accatgacct aataattcag
     1681 tcacgagtaa tcctctattc atttttttca gaagcgcctc aaagccaagc ggttcagcac
     1741 acgacaatat aagattgtga ttaccccctg catttcccgt tgaatgtaag cccagctttc
     1801 ttgctgaata gctattgagg aaataacctt gcaccacacc attctcaata atgctacgat
     1861 ctttcgtcgc gattccttct tcatcaaaag tacagcttgc tagacctttg ggaatatggg
     1921 gtcgctcgtg aagctgaata tcagacggaa gaatttgttt accaatgcta tccaataaga
     1981 acgatgattt acggtataaa ctcccaccac tgatagcgct taccatgtgc cctattaaac
     2041 tcgaggcaag gggcgcttca aataacacgg gtacttcgca cgttgaaagc ttcttggccc
     2101 ctaaacgtgc aaccgtgcgc attcccgctt ttttacccac agcttcaatc tgctccagat
     2161 catgcgcatc tcgtgcaaca ctaaaccaat aatcgcgttg catcatatcg gcctcactcg
     2221 caatcacagc acagctaata ctatgtcgtg aaacaggata tcctcccata aaacccaagc
     2281 tattggcgta tacaaattga gaagcgccaa ctgaaactga ggctccttca gaattcgtaa
     2341 tacgtttgtc gacaacgagc gcctcggctt cacagcgctg agccagttcg atagccgcct
     2401 caacgggtaa attccatggg tgatacaaat cgagatcaga aaatgtggtt gccaataaat
     2461 tagcttctgc aaggccagca caattatctt ctgcagtatg tcgcgctatg gacaatgctg
     2521 cagcgactgt atcattgata gcttggggtg aaaaatcaga agtactggca ttgccccgtt
     2581 tctgacctat ataaacggtt accgataatc ctttatctcg attatgctcg atcgtctcaa
     2641 ctgcattctg gcggacagtg acatttaaac caaaaccatc tgacacgttt gcctcgcagg
     2701 cagttgctcc gcctcgctta gcgtgattca aaatatcatt tgcaacttgc tgcaattccg
     2761 caaaagaata ggaaaaattt tgtccgctcg aagtatctgg attcataccg cctttactta
     2821 tttaaactat gaaatacatt agcaccgcgc taaaagcaac ggtgcgtgaa attcaaaaaa
     2881 aattttacaa aaccgctatg atagcagctt cttcacacag ctataaatcg atctgaaatg
     2941 atacacgata gtcatgatga caatgaatcc gaagaaattc tcagtaagac acgccgtaag
     3001 aaagcaatgc atgcactcca aggaattggt gagcagcttg tcgaattaga ttcccaattc
     3061 attgctgaac tggatttacc agagatattg gtcgatgcga tccgagaagc gaagcaactg
     3121 accaagcatg aagccaaacg acgtcagatg caatatattg gtaaactgat gcgtaatatc
     3181 gacgctgcac cgattcaaga aaaaatcgat tactggaaaa atcgtgacgt taatcatacc
     3241 gcttggttac atcggttgga aaactggcgc acgcggctct tggctgatga gaattcaatt
     3301 aatgaattca aacagcacta tcctgaaact gatatacaac acttacgagc cttgattcgt
     3361 aatgcccata aagaaacact ttcgcagaaa ccacctaaaa gcttccgtgc aatttttcaa
     3421 gaattgcgca cgattattcc agagaaaaaa taaccgaata agttttaagt taatgtgaga
     3481 atccagcaag gttgagttca cgatcttttt tgattgatga taattcaaga ttccaaatgg
     3541 ctgtagctaa agcttgggaa gactggttgg ctgttttgga agaagattcg ttcgatagta
     3601 agctagatgg tcaggaagat ttaattgata atttgacttt gtcttgtcct caaatttttc
     3661 aaaaagattg tttaactcta tattagatgg cgtctagaat ttagatgaaa aataagaatt
     3721 tttaattctt gctgagaagt aaatccaatt atttgatttt attgatctag aaaagaaaaa
     3781 aaacagattt agataatgct tagtatagta atttaaaagc attattcctt cagtttcttt
     3841 tttttgttat acaatcacgc gatacaagcg cgtagttaaa aaactgcgtg acaaattttt
     3901 agagatatta ttgaaaggga gtgtatgaaa aaggtgttac aaaataattg gatagcgaag
     3961 ggattcgtgg ttattttcat ggtattggct tcgttttcat tgaaagcaca gttgatgttg
     4021 gcccatgaag gacatcatgc agggcatggc ggctgtgagg taaagacagg ttctttcgtt
     4081 gtaacagtaa gtatttatga ggtgcctgaa ggtaatattc caccgatgca ttcatattgc
     4141 aatcatgtac ctaaacccgg aaaagtgaat gtaacgatcg agttgccatc agatgctcgt
     4201 gagattccac tagcagttcg tctggtgaaa gatgactcgc atgcaggaca cggaagtgga
     4261 tctcctcaaa gtaatgcaac tgagaaagca gcgagtgctg taaacgaagg agatgatcat
     4321 gcgcatcatc acgatgaaac gcatggcgca ggagagtttg gtctgcatta tatgccgcca
     4381 atggaacatc actctggaat tattgtggtc gcagcggagt taaccgagct agggcaatat
     4441 gcgattttgc ttgaacaaat cgatgataaa gatgatgtga gaatagtttc caaagtgccg
     4501 gttcatgttg gaggtgatgg aggccatgct ggccatggat caggtattgg tttaatggaa
     4561 attgtaattg ggctcatgct ggttgtgggt ggaatcggtg cctttatgtt tagaaagaaa
     4621 aagccacaag caagctaatc taaatttaaa ggtttgagag tgaatcgaac aggaagtaac
     4681 gggaagagtt aagaaaagga ggagagaata gatgaag
//
LOCUS       DAOQLJ010000163         4708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_8586_length_4708_cov_7.810015, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000163 DAOQLJ010000000
VERSION     DAOQLJ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8586_length_4708_cov_7.810015"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2334
                     /locus_tag="PK522_09940"
     CDS             <1..2334
                     /locus_tag="PK522_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC42092.1"
                     /translation="MLPLYWQMLWQVLITIAAATIALYVAYRLLQHLNRSVLNPLNQL
                     SEVIEHVTSNADYKARTPKSNIVELDMLANGFNNMLEVIQERDSKLANHLDHLEDEVS
                     KRTSELVLAKEAAEAASKAKSEFLATMSHEIRTPMNGILGMTELLLNTELSKDQYRFA
                     DTVQRSGQHLLGIINDILDFSKIESGHMELETVDFELVKLIEDTLIMFAQPADEKGLE
                     LAAQFIPPNIPIAIKGDPFRLKQVLVNLINNAIKFTSQGEVIIRTRIIEESETQIHIN
                     ICVADTGIGIPEAYHDKIFQHFSQADGSTTRQYGGTGLGLTICKRLLELMNGRIWVES
                     SSGHGSKFWIDIKMEKCSLAAIAALPNLEILHNTRVLIVDDNQTNREILELQLKSWNV
                     KVNCAENAERALIMMSKAVEDCDPYRLAILDMHMPKMDGLQLAKIIASDANLSKTRLM
                     MLTSTYSNASQLERQNVGILRCANKPVRQRELLEIILDVLQRSDKLAHSETPIKQSTS
                     ALPAKNLTQGKVLLAEDNLVNQEVAKAMLAKLGFKVDIANDGQQALDFMNKQRYDIVL
                     MDCQMPIMDGFEATTQIRKQYGQTIPIIALTANATEDDRNHCIEAGMDDFLSKPYTLN
                     QLNQVLTKWETKTQSNLVDAMSESHVPKENTEIKKMPLLNPVLLDQIRSLDTSNGHEL
                     VNKILCAFLESAEKYIELLDRAFNDKDIETIRKTAHTLKSSSANIGAEELSTIFKQIE
                     AYSKNGELALAESLYSGMLAIYQQVIIEVKAIINQSQ"
     gene            2331..4097
                     /locus_tag="PK522_09945"
     CDS             2331..4097
                     /locus_tag="PK522_09945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC42093.1"
                     /translation="MSRTFKILLADDDVTIRFLMQAALEQAGFEVILASNGEEAIQLF
                     NTSSPDMVMLDVEMPQMNGYQVCTYLRKQIGNELPIIMVTGMDDVQSIDHAFEVGATD
                     FIAKPINWNLINYRLLYLRRAYLNLLDLKNANARSKAIFSAIPDTMFIIDNNGHIVDT
                     QSSSDKNTFLNFQTGDDLNQRMPANITQAHLEAAGRARKYGAVEHFEYQLNGDSDREK
                     YYESRVVAIDSNEILCLVRDITERKDSENRIFRLAYFDSLTGLPNRQSFIDRLKREIK
                     RAKYIGSKLAILFLDLDGFKSINDTMGHNTGDVILQWAADRLQSSTRSSDFVSRSNTT
                     GSEIELARLGGDEFTVVIPNLTRAEDALVLAHRIRETMRRPFRLDSRDVVLTTSIGIA
                     LYPDDGEDVEALLKHADTAMYHAKTEGRDNCQFYSVTLTQEAEKRLNLENDLRNALKN
                     NEFHLLYQPQFDVERSHFKSAEALIRWNHPKQGLVSPLDFIPLAEENGLIIPIGEWVL
                     RTACSDAAHWLKNGGDLKVAVNLSPLQFKNPDLIKSVLSILTETGLPPHKLTLEVTEG
                     ALMEYSENTLATLRELRKNKRL"
     gene            4129..>4708
                     /locus_tag="PK522_09950"
     CDS             4129..>4708
                     /locus_tag="PK522_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISNol1 family transposase"
                     /protein_id="HNC42094.1"
                     /translation="MLRNSKLSDYYIRKIIQCFCIDIPASKTALLLGKNRNTINRWYG
                     IFRQVIYSHQTALKDKLLGKVEVDESYFGAKRHRGYHGKLKRGRGTLKQPVFGVFERD
                     GRVYTEIVPDCKRLTLQAVILGKVSIESVIYSDGWRGYNGLVDVGYSKHFRVSHGDNE
                     FARDGHCHINGIESFWSFTKRRLAKFNGVSVNF"
BASE COUNT         1516 a          896 c          999 g         1297 t
ORIGIN      
        1 atgttgcctt tatattggca aatgttatgg caggttttaa tcacaattgc tgctgcaact
       61 attgcacttt atgttgctta tcgactattg caacatttaa atcgctcagt attgaatcca
      121 ctcaatcagc tctcagaagt cattgaacat gttacgagca atgcagacta taaggcaaga
      181 actcccaaaa gtaatatcgt agagctcgat atgctagcga atgggttcaa taacatgctg
      241 gaagtcattc aagaacgaga ttccaaatta gccaaccatc ttgatcattt agaagatgaa
      301 gtttcaaaac gaacatcaga gttggttctc gcaaaagaag cagcggaagc agcgagtaaa
      361 gccaaaagtg agtttcttgc tacgatgagt catgaaatac gcacacccat gaatggcata
      421 ctgggaatga cagaattgtt actgaatact gagctatcta aagatcagta tcgatttgcg
      481 gatactgtgc aacgatcggg ccagcatttg ctcggcatta ttaacgatat tctggatttt
      541 tcaaaaattg aatccggtca catggagctt gaaacggttg attttgaact ggtcaaatta
      601 atcgaagata ccctcatcat gtttgctcaa ccagctgatg aaaaagggtt agagttggct
      661 gcacaattca tacctcccaa tataccgatt gcgataaaag gcgacccatt tcgcttaaaa
      721 caagtgcttg tgaatctaat taataatgcg attaaattca ccagtcaagg cgaagtcatt
      781 attcggacac gcataataga agaatctgaa acgcaaattc atattaatat ttgtgttgcg
      841 gatacaggga ttggtattcc tgaggcttat catgacaaaa tattccaaca tttctcgcaa
      901 gcggatggtt caacaacccg ccaatacggt ggaacaggcc ttgggctcac tatttgtaag
      961 cgtttattag agctgatgaa tggtcgaatt tgggttgaaa gtagttcagg gcacggctcc
     1021 aaattctgga tcgatatcaa gatggagaaa tgttcacttg cagccattgc cgctttacca
     1081 aatctagaaa tactgcacaa taccagagta ttaatcgttg atgacaatca aacaaatcgt
     1141 gaaatactag aattacaatt aaaaagttgg aatgtaaaag taaattgtgc tgagaatgcg
     1201 gagcgagcac taatcatgat gagcaaagca gtagaagatt gtgatccata tcggctagct
     1261 atcctcgata tgcatatgcc taaaatggac ggccttcagc ttgcaaaaat tattgcatcc
     1321 gatgctaacc tgagcaaaac tcgcctcatg atgcttacct ctacctatag caatgcctca
     1381 caattagagc gacaaaatgt tggcattcta cgctgcgcta ataagccagt acgacaacgg
     1441 gaactactcg aaatcatcct agatgtcttg caacgctctg ataaattagc ccattcagaa
     1501 acaccaataa aacaatctac ctcagcatta ccagcaaaaa acctgacaca aggcaaggtg
     1561 ctactggctg aagataactt agtcaatcaa gaagtagcga aagcgatgct tgctaagtta
     1621 ggtttcaagg tcgatattgc taacgacggt cagcaagcgt tagattttat gaataaacaa
     1681 cgttacgata tcgttctcat ggattgccaa atgccaatca tggatggatt tgaagcgacg
     1741 acccagattc gcaaacaata cggtcaaaca ataccgatta ttgcgttgac tgctaacgcg
     1801 actgaagatg atcgtaacca ttgcatcgaa gcaggtatgg atgattttct ttcaaaaccc
     1861 tatacattga atcagttaaa tcaagtcctc actaaatggg aaacaaaaac tcaatcaaac
     1921 ttagtcgatg caatgtcaga atctcatgta ccaaaagaaa atactgaaat aaaaaaaatg
     1981 cctttattga atcccgtatt gttagatcag atccgtagcc ttgatacatc aaatggacac
     2041 gaattagtca ataagatttt atgcgccttt ctcgagtctg cagaaaaata tatcgagtta
     2101 cttgaccgag catttaatga taaagatatt gaaaccatta ggaaaacagc acacacatta
     2161 aaatcaagtt cagctaacat tggtgctgag gaattatcaa caatattcaa acaaattgaa
     2221 gcatatagta aaaatgggga attagcgctc gctgaatcgt tgtattcagg gatgttagct
     2281 atttatcagc aagtaataat cgaagttaaa gcgatcatca atcaatctca atgagcagaa
     2341 cttttaaaat actacttgct gacgatgatg taacgattcg ctttttgatg caagccgctt
     2401 tggaacaagc aggctttgaa gttattttgg caagtaatgg ggaagaagcg attcagttat
     2461 ttaatacatc ctcacccgat atggtgatgt tagatgttga gatgccccaa atgaatggtt
     2521 accaagtgtg tacttaccta cgtaagcaaa ttggcaatga acttccgatt atcatggtca
     2581 ccggtatgga tgatgttcaa tcaatcgatc atgcatttga agtgggcgcc actgatttta
     2641 ttgcaaaacc aattaactgg aatctgatca attatcgatt gttatatctc aggcgggcct
     2701 acctcaactt gttggatttg aagaatgcaa atgcccgtag caaagctatt tttagcgcca
     2761 ttcccgacac gatgttcatc atagataata atggccatat cgttgataca caaagtagtt
     2821 ctgataaaaa taccttttta aatttccaaa cgggagatga tttaaaccag cgaatgcctg
     2881 caaatattac ccaggcccat ttggaagcag caggaagagc ccgtaaatat ggcgcagtcg
     2941 aacactttga gtatcaatta aatggcgata gtgatcgtga aaaatactac gaaagtcgtg
     3001 ttgtagccat tgattccaat gagatcttat gcttggtacg ggatattacg gagcgaaaag
     3061 attctgagaa tcgaatcttt cggttagctt attttgatag cttaaccgga cttcctaatc
     3121 gtcaatcgtt tattgatagg ctaaaacgcg aaataaaacg tgcaaaatat atcggctcga
     3181 aacttgctat cttatttctt gatctcgatg gcttcaaaag tattaatgac acaatgggcc
     3241 ataacaccgg ggacgttatt cttcagtggg ctgcagatcg acttcaaagt agcactcgct
     3301 cctctgattt tgtatctcgt agcaacacaa ctggctccga aatagagctc gcacgattgg
     3361 ggggagatga attcactgtc gtaatcccga acctgacacg ggcagaagat gcattggtac
     3421 ttgcgcatcg aatacgtgag accatgcgac gtccattccg attagacagc cgcgatgtag
     3481 tactcaccac aagtattggg attgcactgt atcccgatga tggtgaagat gttgaagcat
     3541 tacttaaaca cgctgataca gcaatgtatc atgcaaaaac ggaagggcga gataattgcc
     3601 aattttacag tgttacgctg acacaagaag cggaaaaacg cctgaattta gaaaatgatt
     3661 tgcgtaatgc attaaaaaat aatgaattcc atttgctata tcaacctcaa tttgatgttg
     3721 agagatcaca ctttaagtcc gctgaagcgt tgattcgctg gaatcatccc aagcaaggat
     3781 tagtctctcc cttagatttc atcccactcg ccgaggaaaa tggcttgatc ataccgatcg
     3841 gagagtgggt attaagaact gcctgtagcg atgcggccca ttggctaaaa aatgggggtg
     3901 atttaaaagt tgctgtcaac ttatctccgt tacaatttaa aaaccctgat ttaatcaaat
     3961 cagtgctttc gattttaact gagacagggt tgccgcctca taaattgacc cttgaagtta
     4021 ctgaaggcgc attaatggag tatagtgaaa atacccttgc tacgctgcgt gaattacgta
     4081 aaaataaaag gctctagact agcagacaca atctgttagt ctagtaagat gttaagaaac
     4141 agtaaattaa gtgattatta cattagaaaa ataattcaat gcttttgcat tgatatacct
     4201 gccagcaaaa cagctctgct gttaggtaaa aatcgtaaca cgattaatcg ttggtatggc
     4261 attttcaggc aagtaatcta tagccatcag actgctctta aagacaagct gttaggcaaa
     4321 gtggaagttg atgaaagcta ttttggtgcg aaacggcatc gaggttatca tgggaaactc
     4381 aaacgcggtc gcggaacatt aaaacagcct gtatttggtg tgtttgagcg agatggcagg
     4441 gtatacactg agatagtacc ggactgtaag cgcttgactt tacaagcggt gatactgggt
     4501 aaagtgtcca ttgagagtgt catctatagt gatggctggc gtggttataa tggtttggta
     4561 gatgtaggat attccaagca ttttcgtgta tcccatggtg ataatgaatt tgcgcgggat
     4621 gggcattgcc atattaacgg tattgaatcg ttttggagtt ttaccaagcg gcggctggca
     4681 aagtttaacg gtgtgtcggt taactttg
//
LOCUS       DAOQLJ010000164         4626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_8823_length_4626_cov_4.664187, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000164 DAOQLJ010000000
VERSION     DAOQLJ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8823_length_4626_cov_4.664187"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PK522_09955"
     CDS             complement(<1..290)
                     /locus_tag="PK522_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42095.1"
                     /translation="MKALLDLFKQVTHKEEFDAIKIGLASPEKIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTLSKV"
     gene            complement(382..4458)
                     /gene="rpoB"
                     /locus_tag="PK522_09960"
     CDS             complement(382..4458)
                     /gene="rpoB"
                     /locus_tag="PK522_09960"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNC42096.1"
                     /translation="MSYSFTEKKRIRKSFAKRASVLPIPFLLATQLESYAAFLQENVA
                     PNKRQNQGLQAAFNSIFPIESHTKNARLDFISYELGVPVFDVKECQQRGLTYASPLRA
                     RVRLTIMDKEISKPTVKEVKEQEVYMGEIPLMTETGSFVINGTERVIVSQLHRSPGVF
                     FEHDRGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDCLFFRIDRRRKMPVTALLKAIG
                     CTSEQILQEFFIFDCFHLSDKGIQFELVPERMRGEVASFDIVVGKNKKVIVQKDKRIT
                     AKHIREMQEVGIDQLEVPDDFLLGRILAQSIVDKETGEFVALANDEITEEALTKIRKA
                     GVKKIHTLYVNDLNYGAYISQTLRIDETTDEMNAQVAIYRMMRPGEPPTEEAVKALFA
                     GLFYSPERYDLSVVGRMKFNRRVGRTELTGSQTLSNEDIIAVIKILVELRNGRGEIDD
                     IDHLGNRRVRSVGELAENQFRSGLVRVERAVKERLSQAESENLMPHDLINAKPVSAAA
                     REFFGSSQLSQFMDQTNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCP
                     IETPEGPNIGLINSLALYARTNEYGFIETPYSKVKDSRVTEEIDYLSAIEEGNFIIAQ
                     ANAELDENHRLISDIVSCRYKNEFALSSPDRIEYVDVAPAQIVSVAASLIPFLEHDDA
                     NRALMGSNMQRQAVPCLRAEKPYVGTGIERVVAVHSGTTVRAKRGGIVDYVDASRIVV
                     RVHDAETRMGEVGVDIYNLIKYTRSNQNTNINQRPLVKVGDDISRDDVIADGASTDMG
                     ELALGQNMLVAFMPWNGYNFEDSILISERVVAEDRFTSIHIEELSVVSRDTKLGTEEI
                     TADIPNLSEYQLSRLDESGIVYIGAEVEAGDVLVGKVTPKGETQLTPEEKLLRAIFGE
                     KASDVKDTSLRVPSGISGTVIDVQVFTREGIERDKRAQKIIDDELDRYKKDLADQLRI
                     VEEDAFARIERLLTGKIATGGPHKLAKGKEITTEYLKSFDPHYWFDIRLADEDASIQL
                     EQVRESMAQKRKAFDAAFEEKKKKLTQGDELAPGVQKMVKIYVAIKRRLQPGDKMAGR
                     HGNKGVISKIVPLEDMPYMADGTPVDVVLNPLGVPSRMNVGQILEVHLGWAAKGLGKK
                     IDEMLRTQRNVNEIREFLDKIYNQSGKKEDLLSLSDNEILALAENLTQGVPFATPVFD
                     GATEEEIKDMLELAGLPRSGQITLYDGRTGEAFDRPVTVGYMHVLKLHHLVDDKMHAR
                     STGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVTGRTKV
                     YESIVKGDHKIDAGMPESFNVLVKEIRSLGMDIDLEQY"
BASE COUNT         1260 a         1188 c          830 g         1348 t
ORIGIN      
        1 actttggata gcgtcacttc aacgccgcat ttttcacaaa taacaccgcg atgctttaag
       61 cgcttgtatt taccgcacaa gcactcataa tctttcaccg gcccaaagat cttggcgcaa
      121 aacagaccgt ccctttccgg tttaaatgtg cggtaattaa tcgtttccgg tttttttact
      181 tcaccataag accatgagcg tattttctcg ggagatgcaa gaccaatctt gatggcatca
      241 aattcttctt tatgagtaac ttgtttaaat aaatctagca gtgctttcat ttgtcactcc
      301 tgttaatcag ggggcaataa taattatatg aatcggtaca aatgcctaaa aaactcgctc
      361 gatcacccag attcgggaca atcaatattg ttctaaatca atatccattc ctagcgaacg
      421 tatttctttt accagcacat taaacgattc cggcattcca gcatcaatct tatgatcgcc
      481 cttgacaata ctttcatata ctttagtacg cccagtgacg tcgtccgatt tcacagtcaa
      541 catttcctgc agggtataag ccgctccata agcttccagc gcccaaacct ccatttcacc
      601 aaagcgctgt ccgccaaatt gcgctttacc gcccaatggc tgctgtgtca ccaaactata
      661 tggaccggta gagcgcgcat gcatcttatc atccactaaa tggtgaagtt tcagaacgtg
      721 catgtagcca actgtgactg gccgatcaaa agcttccccc gtcctgccat cgtacaatgt
      781 aatctgacct gatcgcggca aacctgccag ttccaacatg tctttaattt cttcctcggt
      841 tgctccatca aaaacaggcg ttgcaaaggg aacaccttgg gttaaatttt cggctaatgc
      901 caaaatttca ttatctgata gtgacaacaa atcttccttt ttaccactct ggttgtaaat
      961 tttatccaag aattccctga tttcatttac attcctttgc gttctcaaca tttcatcgat
     1021 tttcttgccc aacccctttg ctgcccaccc caaatgcact tcaagaatct gtcctacatt
     1081 cattcgagag ggaacaccca atggattcag cacgacatcg acgggtgttc catccgccat
     1141 atagggcata tcttccaaag gaacaatttt agagattaca cccttattgc cgtggcgccc
     1201 tgccatctta tcgccaggct gcaagcgccg cttaatggca acataaatct ttaccatttt
     1261 ctgcacaccg ggtgccaatt catctccctg tgtgagtttt ttctttttct cttcaaatgc
     1321 cgcatcaaac gctttgcgct tttgcgccat gctttcacgc acttgctcta actgtatact
     1381 cgcatcttca tcggctaagc gaatatcaaa ccaataatgc ggatcaaagc tttttagata
     1441 ttcagtggta atttccttcc ctttagccaa tttatgtggt ccaccggttg caattttacc
     1501 ggtcaacaat cgctcaatac gcgcaaatgc atcctcctcc acgatacgca attgatcagc
     1561 caaatccttt ttataacgat ctagttcatc atcaataatt ttttgcgcgc gtttatcacg
     1621 ctcgatacct tctcgcgtga acacttgcac atcgataact gttcctgaaa ttcccgatgg
     1681 cactcgcaat gatgtatctt tcacatcgga tgctttttca ccgaaaatcg cacgtagtaa
     1741 tttctcttcc ggtgtcagct gtgtttcacc tttaggagta accttgccaa ccaatacatc
     1801 gcctgcttca acttctgcac ctatatacac aataccagac tcatcaagtc gactgagttg
     1861 atattcggat aaattaggaa tatcagccgt tatctcctcg gtaccaagtt tggtatctcg
     1921 gcttactacg gacagctctt caatatgtat cgatgtaaag cgatcttcag ccacaactcg
     1981 ctctgatatt aaaatggaat cttcaaagtt ataaccattc cacggcataa atgccaccag
     2041 catgttctgt cccaatgcaa gctcccccat atcggtggaa gcaccatcgg ctatgacgtc
     2101 atctctgcta atatcatcac caacttttac taaaggtcgc tgattgatat tggtattctg
     2161 atttgaacgc gtatacttta tgagattata aatatccacc cctacttcac ccatgcgagt
     2221 ttctgcatcg tgcactcgca ctacaatccg gctagcatca acataatcta caattccgcc
     2281 acgtttagcc cgcacggttg ttcctgaatg cacggctact acacgttcaa taccggtgcc
     2341 gacatatggt ttttcagcac gcaagcacgg aaccgcttgg cgctgcatgt ttgatcccat
     2401 caaagcacgg ttagcatcat cgtgttctag aaagggtatc agcgaagcgg caacagaaac
     2461 aatctgcgct ggtgccacgt ccacatattc aatccgatcc ggcgacgata atgcaaattc
     2521 atttttgtag cggcaggaaa caatatcgct aatcaatcga tgattctcat caagctcggc
     2581 gttagcttga gcaattataa aattgccttc ttcgatagca gacaaataat cgatttcttc
     2641 cgttacccgg ctatctttca ctttactata aggtgtttca atgaatccat attcgttggt
     2701 tcgcgcatac aatgccagcg aattgatcaa accgatattc ggtccttccg gagtttcaat
     2761 cggacacact cgcccataat gcgttggatg cacatcacgc acctcgaaac cggctctttc
     2821 acgcgtcaag cctcccggcc ctaatgctga aatacgccgc ttatgtgtga tctcagataa
     2881 aggattcgtt tgatccataa attgcgataa ctggcttgaa ccaaagaatt ctcgggcggc
     2941 cgctgaaacc ggctttgcat taatcagatc atgcggcatc agattttccg attctgcctg
     3001 gcttaagcgc tctttcactg cacgttctac acgcaccagt cccgatctaa attggttttc
     3061 cgccaactca cccacagagc gcacccggcg attaccaaga tgatcgatat catctatttc
     3121 gccgcggcca ttgcgcagtt caaccagaat tttaataacc gcaataatat cctcattgga
     3181 cagcgtctgc gatcctgtca actcggttct acccactctg cgattaaatt tcattcgtcc
     3241 cacaacagat aaatcataac gttcagggga gtagaataag ccagcaaaca gcgccttaac
     3301 agcttcttct gtcggcggtt caccggggcg catcatgcga taaatcgcaa cctgcgcatt
     3361 catctcatcc gttgtttcat caattctcag tgtctgggaa atataagcgc cataattcaa
     3421 atcattcaca tagagcgtat gaattttctt tacgccagct ttacgaattt tagtcaatgc
     3481 ttcttcagtg atttcatcat tggcaagcgc gacaaattcg ccagtttctt tatcaactat
     3541 actttgtgct agtattctcc ccaataaaaa atcgtcgggc acttccagct gatcaattcc
     3601 aacctcttgc atttcccgga tatgcttagc cgttatgcgc ttatccttct gaacaatgac
     3661 tttcttgttc ttacccacaa caatatcaaa actcgccact tcacctcgca tgcgttctgg
     3721 cacgagttca aattgaattc ctttatctga taaatggaaa caatcaaaaa tgaaaaattc
     3781 ttgtaatatc tgttcagagg tgcaaccaat tgctttcaac aaagccgtca caggcatttt
     3841 gcgtcggcga tcaatcctga aaaacaagca atcctttgga tcgaattcaa agtccaacca
     3901 cgatccgcga taaggaataa ttcgcgcgga aaacaataat tttcctgaag aatgagtttt
     3961 accacgatca tgttcgaaaa atactcccgg agaacgatga agttgcgaaa caatcacacg
     4021 ctctgtgcca ttgataacaa acgatcctgt ctcggtcatc agaggtattt cgcccatata
     4081 tacttcttgc tctttgactt ccttgactgt cggcttggat atttctttat ccataatcgt
     4141 cagcctaact ctagcgcgta gaggcgatgc ataagtaagt ccgcgttgtt gacattcctt
     4201 gacatcgaat accggcactc caagctcata gcttatgaaa tcaaggcgtg cattcttcgt
     4261 atgactttca attggaaaaa ttgaattaaa agcagcttgt aatccctgat tctggcgctt
     4321 gttaggtgca acattttctt gtaaaaatgc tgcgtatgat tcgagctgag tggcaagaag
     4381 aaacggtata ggtaatacac tcgcacgttt ggcaaaactt ttacggatgc gttttttctc
     4441 tgtgaacgaa tagctcatga attttctccg agagaagaag acagaggatt gaaggatcga
     4501 ggccaaataa ctatcaattg ttcaaatgcc caaaatgaca catcaaatgg tcaaaatatc
     4561 aaaggctggt ggctttaata gcctaccagc ccagaaaatt tataattttt attacttaat
     4621 ttcgca
//
LOCUS       DAOQLJ010000165         4518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_9178_length_4518_cov_5.562850, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000165 DAOQLJ010000000
VERSION     DAOQLJ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9178_length_4518_cov_5.562850"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="PK522_09965"
     CDS             complement(<1..1355)
                     /locus_tag="PK522_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HNC42097.1"
                     /translation="METKAVKKYSQKWLPILQLLLISIFYQNAVAVAETIEGEQSFRN
                     SINAINVVNATNGQITVKITLDQPMSSPPVGFSLTNPDRIVFDFTNSINGLGRNTQEV
                     GNSDLRSINIVQTENRSRLVMNLLRPMTHHAKVQDNYVVIILKAVNLSSVAVTPTLSK
                     IINSSENNLKSLQDIDFRRGANGEGRIEVLLSEPGTAVDVHTQGERIVVEFVKTYLPP
                     NLARRLDVLDFATPVQIIETLTKGDNVQLVVEPKGRWEHTSYQSGTRFFLEVKPVIES
                     EDVPAHKKLMNGGYTGEKLSLNFQNVEVRSVLQVIADFTNLNIIASDSVKGSLTLRLK
                     DVPWDQALDIVLQSHELDKRRVGNVIFVAPREELAAREKMDLESRQQISELEPVQTET
                     FQLNYRTASSIPLTGILSQRGTVEMDDISNTLTVTDIPIRLAEVAKHVKNLDTPVRQV
                     M"
     gene            complement(1355..1879)
                     /locus_tag="PK522_09970"
     CDS             complement(1355..1879)
                     /locus_tag="PK522_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HNC42098.1"
                     /translation="MNDRIRLLLIGFVIVLTGCEANTHSDLEEFVNHSSEGLHGTVEP
                     LPEIQTITQFTYAAFELPNPFSPRKNNQVKENQGSIQPDFKRRKEVLESFPLENLAMV
                     GTLQRDRQIYALIKAPDSTLHRVKVGNYLGQNFGLITGISEAEIKLREIIQDSASDWT
                     EHPSTLMLQAQEQR"
     gene            complement(1876..2502)
                     /locus_tag="PK522_09975"
     CDS             complement(1876..2502)
                     /locus_tag="PK522_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNC42099.1"
                     /translation="MSLLEELQRINPNDPGNWPAKIKAGALIILLCLLIFAGYFFIWE
                     EQWVELENAETEEKTLKENFLTKKKSAVNLVVMQQQLIEIENSLSAMLKQLPNKSELD
                     ALLVDINRAGLGRGLQFELFKPAASELIKEFYAELPVTVRVTGNYHDIGAFASDVSQL
                     SRIVTLHDIEILPGKDQQLVLSATAKTFRYLDDEELASQGKAPSGGKK"
     gene            complement(2519..3085)
                     /locus_tag="PK522_09980"
     CDS             complement(2519..3085)
                     /locus_tag="PK522_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNC42100.1"
                     /translation="MIRINLLPHRELKRKARQQQFAILAGMTCALGLFIVWGMYEYIA
                     GEIEHQNGRNQYLQNQIAILDKQIEEIKRIKEQKQELIARKGVVEALQLDRAKVVHVL
                     DQVARLMPDGVYLKSLKQTEQHIHLSGYAQSNAWVSTLMRNLDASPWFESPLLIEIKA
                     ATINNSRSNEFDLNVRLSESPNSLTEQL"
     gene            complement(3082..4188)
                     /locus_tag="PK522_09985"
     CDS             complement(3082..4188)
                     /locus_tag="PK522_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNC42101.1"
                     /translation="MLGKTFNLDLNLFFPQSPRLLGIDISSSSVKIIELSKIKKKSTN
                     TYRLERYVIEPMSKEAIIDGNIQNLEAVGESIRKGWKKTGSRLKEAALALPAAAVITK
                     KVVLPAGQREEDMVFQVETEANQYIPFSLDEVNLDFQVLGPVPDHPEEVEVLIAASRK
                     EKVEDMVAAVQTAGLKAVVMDVEPFAAQAAFELMKHQLPEGGKNQVIALVDIGASITR
                     INIIHDGESVYARDQPFGGDQLTQEIQNQFNLSVEEAEAAKRKGTLPENYGQDVLQPF
                     CETLALEIARAFQFFFTSTQYMQVDCIFLSGGCATIPGLEAIIAARTQTITQIANPFF
                     NMELSNRVSQRQLKQDAPLLLIACGLAMRSFDPS"
BASE COUNT         1232 a         1022 c          829 g         1435 t
ORIGIN      
        1 atcatcactt ggcggacagg cgtatctaga tttttgacat gcttagcaac ttcggccagc
       61 cgaatcggaa tatccgttac cgttaaagta ttactgatat catccatctc aacggttcca
      121 cgctggctca atatcccagt caatggaatt gagcttgcag tccgataatt caattgaaat
      181 gtttcggtct gaactggttc taactctgaa atctgctggc gagattccag atccattttt
      241 tcacgagcag ccaattcttc tcttggtgca acgaaaataa cgttaccaac ccttcgctta
      301 tcgagctcat gggattgcaa tacgatatca agggcttgat cccagggcac atcttttaac
      361 cgaagtgtca ggcttccttt tactgaatca ctcgcaataa tgttcaagtt agtaaaatcc
      421 gcaattactt gcagtactga acgaacttct acattctgga aattcaggga tagcttttct
      481 cctgtataac ctccattcat gagcttctta tgcgctggca catcctcgct ctcgataaca
      541 ggtttcactt ccagaaaaaa acgtgttcct gactgatagg atgtatgctc ccaacgccct
      601 ttaggttcaa caacaagctg aacattatcc cctttggtca gcgtctcaat aatttgtact
      661 ggggtcgcaa aatcaaggac atccagtctt cttgctaaat taggtggtaa ataggtttta
      721 acaaactcga caacgattcg ctccccttgc gtgtgaacat cgaccgcagt acctggttct
      781 gatagtaata cttcgatacg cccttcacca ttagcgcccc gtctaaaatc aatatcttgt
      841 aaacttttta aattattctc acttgaatta ataatttttg atagcgttgg tgtaactgcg
      901 acactactta aattcactgc tttaagtatg attacaacat aattatcttg aacctttgca
      961 tgatgagtca tgggacgcaa taaattcatg accaagcgac ttctattctc tgtttgcaca
     1021 atattaatgc tacgcaaatc actgttacca acttcctgcg tattacgtcc caatccatta
     1081 atactattgg taaaatcaaa tacgattcga tcaggattag tcaatgaaaa ccctactggc
     1141 ggagaactca ttggctgatc tagtgtgatt ttgaccgtaa tctgcccatt cgtagcattc
     1201 actacattga ttgcattaat gctattacga aaactctgct ctccctcgat cgtctcagca
     1261 actgcaacag cgttctggta aaaaatactt atcagcaata attgcagtat gggcagccat
     1321 ttttgagaat attttttcac tgcttttgtc tccattatct ctgctcctgg gcttgtaaca
     1381 taagtgtact tggatgttcc gtccaatcgc ttgcactatc ctgaataatt tctctcaatt
     1441 tgatttcagc ttcagaaatt ccggtaatca atccaaaatt ttggcctagg taattcccta
     1501 ctttgacacg gtgcagggtg ctatcgggcg ctttaatcaa tgcgtaaatt tgtcgatcac
     1561 gctgcaacgt ccccaccatt gctaaatttt ccaagggaaa actctccagc acctctttac
     1621 gacgcttgaa atcaggctga atcgatcctt gattttcttt gacttgatta ttcttcctgg
     1681 gtgaaaaggg attcggtaac tcaaaggctg cataggtaaa ctgagtaata gtttgaattt
     1741 caggcaatgg ttcaacggtc ccgtgtagac cttcactcga atggttcacg aattcctcaa
     1801 gatcagaatg ggtatttgct tcacatccag tcaatacgat aacaaaccca attaataata
     1861 aacgaatcct atcgttcatt tcttgcctcc actcggggct ttaccttgac ttgcgagctc
     1921 ctcatcatca agatagcgaa acgtttttgc cgtggcgctt aatacgagct gttgatcttt
     1981 tccaggcaag atctcaatat catgtagcgt tacaatacgg gataactgag aaacatcgct
     2041 ggcgaaagca ccgatatcgt gataattccc ggtcacccga actgttaccg gtaactctgc
     2101 ataaaattct ttaattaatt cacttgcagc aggcttaaat aattcaaatt gcaggccacg
     2161 ccctaaacca gctctattga tatccaccag taatgcatct agctcggatt tgttaggaag
     2221 ttgtttgagc attgcgctta gcgaattttc aatttcaatt aactgttgct gcattaccac
     2281 taagttgact gcactttttt tcttagttaa aaagttttct ttgagtgttt tttcctcagt
     2341 ttctgcattt tcaagctcga cccattgctc ttcccaaata aaaaaatagc cagcaaatat
     2401 cagtaaacag agcaaaataa tgagtgcgcc cgctttaatt tttgctggcc aatttcccgg
     2461 atcattcggg tttattcgtt gcaattcttc tagtaggctc atagtgcatg cagtgatatc
     2521 aaagctgttc agtcaagcta ttaggcgact cagacaatct gacatttaaa tcaaattcgt
     2581 ttgaacgact attattaata gttgctgcct tgatttcgat gagtaatggc gattcaaacc
     2641 aaggcgatgc atccaagttg cgcatcaagg tagaaaccca cgcattggat tgtgcataac
     2701 cggacaaatg gatatgttgt tcagtttgtt ttaaactttt gagataaacg ccatctggca
     2761 tcagtcgtgc cacttgatcc aagacatgca ccacttttgc tctatcgagc tgtagtgctt
     2821 caaccactcc tttgcgggcg atcagctctt gcttttgttc tttaatgcgc ttaatttctt
     2881 cgatctgttt atcgagaata gcaatttgat tttgtaaata ctgattgcga ccgttctggt
     2941 gttcaatctc tccggcaata tattcataca ttccccagac aatgaacaac cctagcgcac
     3001 aagtcattcc agctagaatg gcaaactgct gctggcgagc cttacgtttt agttcacgat
     3061 gaggaagtag atttattcgg atcatgatgg atcaaagcta cgcattgcta aaccacaagc
     3121 aatcaaaagc agtggtgcat cttgctttaa ttggcgttgt gaaactcggt tcgatagttc
     3181 catattaaag aaagggttcg ctatttgcgt aatcgtttgc gtacgcgcag cgataatggc
     3241 ttctaaacct gggatcgttg cacacccccc cgatagaaaa atacagtcta cttgcatgta
     3301 ctgtgttgac gtaaaaaaga actgaaatgc tcgcgcaatt tctaatgcaa gcgtctcaca
     3361 aaagggctgt aatacatcct ggccgtaatt ttctggtaat gttcctttac gctttgctgc
     3421 ttcagcttct tcaactgata agttaaactg attctgtatc tcttgagtca gttgatctcc
     3481 accaaacggt tgatcacgag cgtaaaccga ctcaccgtca tgaatgatat ttatccgtgt
     3541 aatactcgca ccaatatcga ctagtgcaat tacctggttt ttcccgcctt ctggcaattg
     3601 atgtttcatt aactcaaaag cagcttgcgc tgcaaaaggt tcaacatcca tgaccactgc
     3661 ttttaaaccc gctgtttgaa ctgcagcaac catatcttcc actttttctt tacgagaagc
     3721 tgcaatgagt acctctactt cttctggatg gtcaggcaca ggccccaata cttgaaaatc
     3781 aagattaact tcgtctaagg agaaaggtat gtattgattg gcttctgtct caacctgaaa
     3841 aaccatgtcc tcttctcttt gacctgctgg cagcacgact ttcttagtga ttactgctgc
     3901 tgctggtaaa gcaagagcgg cctctttaag gcgtgaaccg gttttcttcc accccttacg
     3961 aatactctca ccgacagctt caagattttg aatattgcca tcaatgattg cttctttgga
     4021 catcggttca atgacatagc gttccaaacg atacgtattg gttgattttt tcttgatctt
     4081 ggaaagttct attatcttga ctgatgatga gctaatatca atccctaaca atctgggcga
     4141 ttgaggaaaa aaaagattaa gatcaagatt aaaagttttt ccgagcattg atttatttga
     4201 aacaatattt tagagttaca gttgaaaggc agctatttct aagaaatggg atcattgctc
     4261 atcattaatt tcatttagct tacctatcgt agcttcacct aacttgctga gcattctgac
     4321 agatttgatc agtatttaat ttgattaaaa acagtagtaa taccctctaa attggaaatc
     4381 gattcctccc tttattaagc aatccctttc atgtcactcc gttggttaat ctatcccttt
     4441 attgcgcttt tcgccgtcag tttgattggc attttattag ttgtatttgc tgcactcgtc
     4501 atcatcccga ccttacct
//
LOCUS       DAOQLJ010000166         4463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_9374_length_4463_cov_5.208258, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000166 DAOQLJ010000000
VERSION     DAOQLJ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9374_length_4463_cov_5.208258"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PK522_09990"
     CDS             complement(<1..575)
                     /locus_tag="PK522_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42102.1"
                     /translation="MTFEIDTLLPRFRRLIPAVDECSLRIVAETAEFTAVRQDVAIPL
                     MRVIDQGAMITIRDADGLGYAATSDLSDAGLRAALDRARDWARLSKRRSVLSDAILKL
                     PMTKGRYRSSTTKPFNWAMADRIDVLMKESQACKIDARVVDWQASLTTQHIRSALMTA
                     DSLATGDYVLQETDITIPALHVTAYEGGVTQT"
     gene            complement(585..2396)
                     /locus_tag="PK522_09995"
     CDS             complement(585..2396)
                     /locus_tag="PK522_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNC42103.1"
                     /translation="MEQNRGKGEIRKLVALWQFLKPYQLRFFFALIAMVIAAGSVLVL
                     GTALKHVIDEGYVLGDMAALNRTLSIVAIVALVQGVATFIRYYLFTWVCERFVADIRK
                     TVFGHILNLSPTFFEKTRTGEVISRLTNDTQRIETVVIGVFSFALRNFVMMIGGIVML
                     AITSLKLTLIILAAVPLVVTPILLLGRRVRKTSEEMQNRVADSSSYIDETIHEIRVVQ
                     AYTHEAVDRDRFADQIERVFKAGVEAGRYRAAMIASVIVLAFSSIAFVLWIGGRAVIT
                     GEISPGDLSAFLFYAILVANGVMAISETWSELQRAAGASERLMDLLSTSPEIKAPQHP
                     KVLPAQTPLTGEQDNQPYKAPIGCVRFEQVTFYYPSRTETAALQNFSLEIQPGEIVAF
                     TGPSGAGKTTVFQLLLRFYDPQQGRLLVEGVDIRELEPTGLRQHFSIVTQEPIVFADS
                     VLENVRYGRPDATREAVIDACQAAYCMQFIEKLPHGLDTQLGERGTKLSGGQRQRIAL
                     ARAFLADRPILLLDEATSSLDAESEQMVQQAMDKLIHTRTTLVIAHRFSTIQSADRII
                     VMDHGTIQAMGTHRELIEQGGLYARQAALQLVTEQKF"
     gene            2867..3343
                     /locus_tag="PK522_10000"
     CDS             2867..3343
                     /locus_tag="PK522_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNC42104.1"
                     /translation="MALTLTSPAFAHNGLIPAQFTCDSQDLSPALAWSGLPEGTKSLV
                     LIVDDPDAPDPAAPKMTWVHWVLYNIPPQVAGLAEAIAPAQLPAGTLEGINDWKRTGY
                     GGPCPPIGVHRYFHKLYALDTVLPDLKQPTKTQLEKSMQGHILGHVELVGRYQRNQ"
     gene            complement(3369..4256)
                     /gene="argB"
                     /locus_tag="PK522_10005"
     CDS             complement(3369..4256)
                     /gene="argB"
                     /locus_tag="PK522_10005"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNC42105.1"
                     /translation="MSLSPPTAKEKAKTLAEALPYIQRFHGKTIVIKYGGNAMTEEDL
                     KRGFARDVVLLKLVGLNPVVVHGGGPQIDQMLKRVGKEGVFVQGMRVTDAETMDVVEM
                     VLGGLVNKDIVNLINRHGGRAVGLTGKDGSFIRAKKMLIEDKEKAGGWINIGQVGEIE
                     SIDPALIALLDTRDFIPVIAPIGVGEDGQSYNINADLVAGKLAEVLKAEKLILLTNTP
                     GVLDKNGNLLTGLTTKRVDELFADGTISGGMLPKISSALDAVRNGVRSCHIIDGRVPH
                     ALLLEILTDEGVGTLISSV"
BASE COUNT         1196 a         1156 c          961 g         1150 t
ORIGIN      
        1 gtctgggtga ctccgccctc ataagcggta acatgcaacg ctggaatggt aatatccgtt
       61 tcctgcaata cataatcacc agtcgcaagg ctatccgcgg tcatcagtgc gctgcggata
      121 tgctgtgttg tcaaactcgc ttgccaatca acgacgcggg catcgatctt gcacgcttgc
      181 gattccttca tcagcacatc gatacgatca gccatagccc aattgaacgg cttagtcgtc
      241 gacgaacgat aacgcccttt cgtcatcggc aatttcaaaa tggcatcact caacacgcta
      301 cgccgcttgg ataaacgtgc ccaatctcga gcccgatcaa gtgctgcacg caatcctgca
      361 tcactcaaat cagaagtggc tgcataaccc agtccgtctg catcacgaat ggtgatcatg
      421 gcaccctgat cgatgacacg catcaaaggt attgcaacat cttgcctgac ggcggtgaat
      481 tcagcggttt ctgcaacgat acgtaacgag cattcatcga ccgctggaat caatcgtcta
      541 aaacggggaa ggagggtatc gatttcaaaa gtcatgtatt ttcctcaaaa tttttgctca
      601 gtgaccaact gtaatgcagc ttggcgagca tagagtcctc cttgctcgat taattcgcgg
      661 tgggtaccca tcgcttgaat agtgccatga tccatgacga tgatgcgatc cgccgattgt
      721 atcgtggaaa aacgatgggc aatcaccaac gtggttcggg tatggatcag cttatccatg
      781 gcttgctgaa ccatttgttc actctctgca tcgagacttg aggtcgcttc atcgagcagc
      841 aatatcggtc gatcggccag gaatgcacgt gctaaagcaa tccgttgccg ctgcccaccg
      901 gagagtttag tgccacgctc accgagttga gtatcaaggc cgtgaggtaa tttctcaata
      961 aactgcatgc aataagctgc ctgacacgca tcgattactg cttctcgcgt tgcgtccggt
     1021 cgaccatagc gcacattctc cagtacgcta tccgcaaaaa cgataggttc ttgcgtaaca
     1081 atcgaaaaat gttgtcttaa tcccgttggt tcaagttccc gaatatcgac cccttcaacc
     1141 aacaagcgac cttgttgcgg atcataaaaa cgcaacagta attgaaatac tgtggtttta
     1201 cctgcgcccg atggtcctgt aaatgccacg atctctcccg gttgaatttc tagcgagaaa
     1261 ttttgcaatg cggccgtctc ggtccgactg ggataataaa aagtaacttg ttcaaaacgc
     1321 acacatccaa tcggcgcttt gtaaggttga ttatcctgtt caccggttaa aggggtttgc
     1381 gctggtaaca ctttaggatg ttgaggcgct ttaatttcgg gcgaggtgga aagcaaatcc
     1441 attaatcgtt cagatgcccc tgccgcgcgt tgcaattcac tccatgtttc agaaattgcc
     1501 atcacgccat tcgcaaccag tattgcatag aatagaaacg ccgatagatc cccgggcgaa
     1561 atctcaccgg taatgaccgc acgcccaccg atccaaagca caaacgcgat ggatgagaaa
     1621 gccagtacga ttacactcgc gatcatcgcg gcacgatagc gcccagcttc gacacccgct
     1681 ttaaatacgc gctcaatttg atcagcaaag cgatctctat ctactgcttc atgggtataa
     1741 gcttgcacga ctcgaatctc atgaatggtc tcatcgatat aactcgacga atccgcgact
     1801 cgattttgca tttcctccga cgttttacga acgcgacgcc ccagcagtaa gataggggtt
     1861 accactaaag gaacggctgc cagtatgatg agcgtcaatt tcaaactggt gatcgcaagc
     1921 atcacgatac cgccgatcat catcacaaaa ttgcgcaacg caaaggaaaa aacaccaatc
     1981 acaacggtct caatgcgttg agtatcgttg gtcaatcgtg aaataacttc cccggtgcgg
     2041 gttttctcaa aaaatgtagg cgataggttg agtatgtgac cgaacaccgt tttgcgaata
     2101 tcggctacaa accgttcaca aacccaggta aacagataat agcgaatgaa ggttgcaacc
     2161 ccttgcacaa gagcaactat cgctacaatc gataatgttc ggttgagcgc tgccatatct
     2221 ccgagtacat acccctcatc gatgacgtgt ttcagcgcgg ttcctaacac aagcactgag
     2281 ccagcagcaa tcaccatagc gatcagcgca aaaaagaaac gcaattggta gggtttcagg
     2341 aactgccaca aagcaacgag cttacggatt tccccctttc ctctgttttg ttccattgaa
     2401 tagtaacgct tcaccatcgt cttaaaaaat ccgagtgatt gaaaaagtta ttaataggca
     2461 tgttctcaat tgtcaaaagc ttatcgatta ttcatccctc gatgaacgct gtgatgttat
     2521 atgggtttat cgcatagcct atcttatcaa attggctgcg attgttgtcg actacagcct
     2581 ggttctacat cacactctgc agatcatcag caattaattc aagcgcgtcg agataacgat
     2641 tttttagtaa tacgatcggt tataatcttt gctaacaact tgcttgctac cgtcatcaga
     2701 aaggagttta accatgaaag gacgttcgac tgcttattcc cggatcaatg gtaagatcag
     2761 tttatcgaaa aatttcagca ttacgctggc cttgctctgg tttgcgatac tagtcggaac
     2821 atcccaggca gacacgacac tttcacaaaa caatacggag aataccatgg cgcttactct
     2881 gacctcacct gcctttgcac ataatggcct gattccagct cagtttacct gtgatagtca
     2941 agacctttca cccgccttgg cttggtcagg gttacctgaa ggtacaaaaa gtctagtgct
     3001 catcgtggat gaccccgacg cgccagatcc tgcagcacca aaaatgacct gggtacattg
     3061 ggtactctat aacattccgc cacaggtcgc tggattagct gaagccattg cgccagctca
     3121 attacctgca ggaaccttgg aaggaatcaa tgactggaaa cgaacgggtt atggcggtcc
     3181 ttgcccacca atcggtgttc accgctattt tcacaaactt tatgccttgg atacggtttt
     3241 acctgattta aagcagccca ccaaaacgca gctagagaaa tccatgcaag gccacattct
     3301 aggccacgtt gaattagttg ggcgctatca gcgcaatcaa taaatccagc aatagcctag
     3361 ttattgcatt aaaccgaaga aatcagcgtc cccacgccct catcggtcaa gatttccaat
     3421 agcagtgcgt gcggaacgcg gccatcgatg atatggcaag atctaacgcc atttctaacg
     3481 gcatccaacg cggagctgat tttgggtagc atgccgcccg aaatggttcc atctgcaaaa
     3541 agctcatcga cccgctttgt cgtcagaccg gtgagtaaat tgccattttt atccaatacc
     3601 cctggggtat tcgttaacaa aatgagtttc tcagctttca gcacttcagc caatttaccc
     3661 gcaaccaaat cagcattgat gttgtaggat tgaccatcct cgccaacacc aattggagca
     3721 atcacgggga taaaatctcg cgtatcgagc aaggcaatca aagctgggtc aatcgattcg
     3781 atttctccga cttgaccgat attaatccac cctcctgctt tttccttatc ctcaatcagc
     3841 attttcttgg cgcggataaa tgagccatct tttcccgtta gaccgactgc tcgaccgcca
     3901 tgccgattaa tcaaattcac gatatcttta ttgaccaagc caccgagcac catttctacc
     3961 acgtccatag tttctgcatc ggtcacgcgc atcccttgga caaatacacc ttctttcccg
     4021 actcgtttga gcatttgatc gatttgcggc ccgcctccgt gtaccacaac cggatttaat
     4081 cctaccaatt tgagcaacac cacatcacgt gcaaaacccc gctttaggtc ttcttcagtc
     4141 atggcattcc caccatattt gattacgatg gtttttccat ggaaacgctg gatataagga
     4201 agcgcttctg caagcgtttt agctttttct tttgcagttg ggggtgataa agacatggtg
     4261 ttaatccgga gcagtcgtaa aaaaatgact tattctactg caagctgggt taagctaaca
     4321 gcgcgttatc tgtcgatacg gataaacgat tgttcgtgaa tcaatgcccg caaattgagt
     4381 tctagccagg gtcgatcttg cttatcgaca aattgcctgg ctttaatcgt ggcatgagcc
     4441 acatcgtgat cttcaatttg ctt
//
LOCUS       DAOQLJ010000167         4454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_9401_length_4454_cov_6.922482, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000167 DAOQLJ010000000
VERSION     DAOQLJ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9401_length_4454_cov_6.922482"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PK522_10010"
     CDS             complement(<1..698)
                     /locus_tag="PK522_10010"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNC42106.1"
                     /translation="MVASKFQLRPLIAFIHDFLAIIIAWWLAYLFRFNFEIPPHFQAS
                     LVENLLWIVPLQASFFYLFRLYRGIWRYASIPDLKRIFWAVTLSAMVVPFVLFMLKLP
                     GVVPRAVIVLAPLLLMLIMGGSRILYRSWKEQRLYSFKNSERNTVIILGTGSMAVNLV
                     KELAHSPDWQVVGLMGDSPKKQGSRLHGVRVLGKINELDYWVEKLSVRHIIIAMPPTQ
                     TNERRQALEICSEVG"
     gene            complement(704..1693)
                     /locus_tag="PK522_10015"
     CDS             complement(704..1693)
                     /locus_tag="PK522_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC42107.1"
                     /translation="MILGLPFQLFVAPILTFLIAYLTIFWLIRLGNSLNIIDIPNDRS
                     LHAKPIPRTGGLGLIGAIFITGLLFVDIFSVPVFIGLAFVSAISFADDIKPLPVWLRL
                     SIHTIAAMLLVVNLFLDTYSWLFVITVGLGIIWITNLYNFMDGSDGLAGGMTAIGFGY
                     YGIAATLAGDIDFATINFSIAASALAFLRFNFYPAKIFMGDVGAISLGFLAGALGLLG
                     WKNNLWSLWLPLLIFSPFIADATVTFIKRLLRGKKIWQAHNEHYYQRLIKGGLGHRNT
                     ALLAYILMLVVGGTAILSAKFEFAQQSWISSMWGIAYLVIMHAFDRHQSHHNR"
     gene            complement(1858..3075)
                     /locus_tag="PK522_10020"
     CDS             complement(1858..3075)
                     /locus_tag="PK522_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNC42108.1"
                     /translation="MTNKDFRPEKKISIFYPPQRVLMGPGPSDTHSRVLSAMARPTLG
                     HLDPVFTDMMEELKSLMRYAFQTNNLLTFPVSGPGSVGMEMCFVNMIEPGDKVIVCRN
                     GVFGGRMIENVTRHGGEAIIVDDKWGEPVDPQKVEDALKKNPGTKIVAFVHAETSTGV
                     QSDAKTISEIAKRYNCLTIMDTVTSLGGTPIYIDGWGIDAVYSGSQKCLSCPPGLSPV
                     SFSDRVVELVKNRKHKVQSWFMDINLLLGYWGSSRTYHHTAPTNALYGLHEALLMLYE
                     EGLEHSWARHQRNHKALKAGLQTMGIEYVVAEPYRLPQLNAVHVPVGIDEKEVRRRLL
                     ADFNLEIGAGLGDFAGKIWRFGLMGTSSRVENVAFCLNALEVVLADLGMKVERGSAEV
                     AAHQTYAQNPMLG"
     gene            complement(3181..>4454)
                     /locus_tag="PK522_10025"
     CDS             complement(3181..>4454)
                     /locus_tag="PK522_10025"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HNC42109.1"
                     /translation="MPFNRATRTWVYKSAKNKGGIVGFGSTYDLREPGAFIFVNAAFP
                     ILEEDQLPTPPVIIGENYCQYPFQAKSVINISGMSYGAISAPAVRALSLGAAQAGCWL
                     NTGEGGLSAYHREGNCDIIMQIGTAKYGVRDAEGNFSPTRTKAIAEFVKAFEIKLSQG
                     AKPGKGGVLLGAKVNPEIAAIRGIPQYEDSISPNRHYDIRNINELLDKVAYIRELTGK
                     PVGVKTAIGGWHFMNELCETILRRGLDYAPDFLTIDGGEGGSGAAPQTLMDHAGLPIA
                     EALPRVVDSLIESDLKKRIRVIAAGKLVTSAHGAWALCVGADFINSARGFMFSLGCIQ
                     AMRCHLNTCPTGITTHNPRLQHGLVVEEKYLRVANYAMNVNKEINMIAHSCGLKHARE
                     FRREHVRIVETAGKSIALNRLYPYPEIRKKA"
BASE COUNT         1331 a          998 c          925 g         1200 t
ORIGIN      
        1 cctacttccg agcatatttc aagggcctga cggcgttcat tagtctgagt gggtggcata
       61 gcaatgatga tatgcctaac acttaatttt tctacccagt aatcgagttc attgattttt
      121 cctagaacgc gcacaccatg caatcttgat ccttgctttt taggactgtc ccccattaat
      181 cctacaacct gccaatctgg gctatgagca agttccttga cgaggttcac tgccatgctc
      241 ccagtcccaa ggataatgac tgtgttacgt tcggaatttt taaagctata taagcgttgc
      301 tctttccagg atcgatacag tattcgactg cctcccataa tgagcattaa tagcaaaggt
      361 gcaagaacga tcacagcacg aggaacaaca ccgggtagct tcagcatgaa taaaacaaat
      421 ggaactacca ttgcgcttaa cgtaacagcc caaaagatac gttttaaatc aggtatgcta
      481 gcgtaacgcc atatacctcg gtagaggcgg aataaataaa agaacgaagc ttgtagtgga
      541 acgatccaca ataaattttc tacgagtgat gcttggaaat ggggggggat ttcgaagtta
      601 aaacgaaata agtaagctaa ccaccaagcg attatgattg ccaaaaaatc atgaatgaac
      661 gcaatcaggg ggcgtaattg aaatttactt gcgaccattc tagttaacga ttatggtgag
      721 attgatgccg atcgaaagca tgcattataa ctaaataagc tattccccac atgctactta
      781 tccagctttg ctgagcaaac tcaaattttg cagagagtat ggcagtacct ccaacgacta
      841 gcatcaatat ataagcgagt aaagctgtat tacggtgtcc aagaccacct ttgattaatc
      901 gctgataata atgttcatta tgggcttgcc aaattttctt gccacgaagt aaacgcttga
      961 tgaacgtcac tgtggcatct gctataaagg gtgagaagat caacaatggc aaccataatg
     1021 accataggtt atttttccaa cccagcaggc ccaatgcgcc agccaaaaat cccagagaga
     1081 ttgcaccgac atcacccata aatatctttg cggggtaaaa attgaatcgt aaaaacgcca
     1141 atgctgaagc tgcaattgaa aaattgatgg tggcgaaatc aatatctcca gctagtgtcg
     1201 ctgcgattcc atagtaacca aaaccaatag ccgtcattcc tcctgcaagc ccatcagatc
     1261 catccatgaa gttatataaa tttgttatcc aaatgatacc gagaccaacc gtaatcacga
     1321 ataaccagct atatgtatcc aagaataagt taacaaccag cagcattgct gcaatcgtat
     1381 ggatcgataa tcttaaccag acaggtaaag gcttaatatc atcagcaaaa gatattgctg
     1441 acacaaaagc taatccaata aatacgggca ctgaaaaaat atcgacaaac aataacccag
     1501 tgatgaagat ggctccaatt aaacccaacc cacctgtgcg agggatcggt ttagcatgca
     1561 aggaacggtc gttgggtata tcgattatat tgagtgagtt cccaagtcta atgagccaga
     1621 agatcgtcag ataagcgatg agaaaagtaa gaatgggtgc gacaaataat tgaaacggaa
     1681 gtcccaagat catgtgaacg ggattatcct aaaaagtcaa aagaaatagt tcaatcggaa
     1741 aaaatagaat ggtttaaacc taaattggaa tcagaatttg ttaattaaca ttaacgaata
     1801 tcgagaagcc atttcctatc aacaggaaat ggccatgatt atttattagg gagatatcta
     1861 tcctagcatt ggattctgag cataagtttg atgtgcggct acttctgcgc tgcctcgttc
     1921 taccttcata cccaaatctg caagaacgac ttctaatgca tttagacaga aagcaacatt
     1981 ctcaactcgg ctggaagtac ccatcaaacc aaagcgccaa atttttcctg caaagtcccc
     2041 cagtcctgca ccgatttcta aattgaaatc agccaataaa cggcgacgta cttctttttc
     2101 atcgatacca accggaacat gcactgcatt caattggggt aggcggtagg gttcagcaac
     2161 gacgtactcg atacccattg tttgcaatcc tgcttttaat gctttgtgat tgcgttgatg
     2221 tcttgcccat gaatgttcta gaccttcttc atataacatc agtaatgctt catgtaagcc
     2281 atataacgca ttggtcggtg cggtgtggtg ataagttcgc gatgaacccc agtagcctag
     2341 gagtaaatta atatccatga accagctttg aactttgtgt ttacgattct taactaattc
     2401 tacaacacga tcagagaagc tgactggcga tagcccaggg gggcaggaca agcatttctg
     2461 actgccagag taaaccgcat cgattcccca tccatcaata tagatgggag ttccaccgag
     2521 agatgttact gtgtccatta tcgttaggca gttatatcgt ttagcgattt cactgatggt
     2581 ttttgcatct gactgaacac ccgtcgatgt ttctgcgtga acgaaagcaa cgattttagt
     2641 gccagggttt ttcttcaacg cgtcctcgac tttttgaggg tcaaccggtt ctccccattt
     2701 gtcatcgaca ataatcgctt cgccgccatg acgtgtaaca ttttcaatca tgcggccacc
     2761 aaaaacccca tttctgcaga cgatgacctt atcgcctggc tcaatcatat ttacgaaaca
     2821 catttccata ccaactgacc caggcccaga gaccgggaaa gttagtaaat tattagtctg
     2881 gaaggcatat cgcatcaggc ttttgagctc ttccatcata tcagtaaaca cagggtctaa
     2941 atgaccaagc gttggacgtg ccatggcaga cagtacacgg gagtgagtat cagatggccc
     3001 cggtcccatt aaaacgcgct gtgggggata aaagatcgaa attttcttct ctggacgaaa
     3061 gtctttattt gtcattgtta ttcctaatgg gtttaaaaag cagtgattat taaaatattg
     3121 aaccactaat tttatcagaa gtgatcaata atttgaggac tgaatcgtgc caagctcaga
     3181 ttaggctttt ttgcgaattt cagggtaagg ataaagtcta ttcaaagcaa tgctcttgcc
     3241 cgcagtttct acgatacgaa catgctcacg gcgaaactca cgagcatgct ttaatccgca
     3301 cgaatgggcg atcatgttga tttccttatt tacattcatt gcatagttag ctacgcgtag
     3361 atatttttct tcaaccacta acccatgttg taagcgcgga ttatgggtgg ttatgcctgt
     3421 tgggcaagtg ttgagatggc agcgcattgc ttgaatgcat cccagtgaaa acataaagcc
     3481 acgggctgag ttgataaaat ctgcaccgac acaaagcgcc caggcaccat gggcggaagt
     3541 aacgagcttt ccagctgcga taacccgaat acgttttttt agatccgatt caatcagtga
     3601 gtcgacaacg cgcggtaacg cttcagcgat tggtaatcct gcgtgatcca tcaatgtttg
     3661 aggtgcggcc ccgcttcccc cttcaccacc atcgatagtt aaaaaatcgg gggcgtaatc
     3721 taatccacgt cgtagaatag tttcacaaag ctcattcata aagtgccatc ccccaatggc
     3781 agtttttacg cctaccggtt ttcccgttag ttctcgaatg taggcaactt tatcgagcaa
     3841 ttcgttgatg ttcctaatat catagtgtcg gttggggcta atggagtcct catattgggg
     3901 aataccacgg atagcagcga tctcaggatt tacttttgcg ccgagtaata caccaccttt
     3961 tcctggtttg gctccttgag aaagtttaat ctcaaaagct ttaacaaatt cggcgattgc
     4021 tttagtccgt gtcggcgaaa aattgccttc cgcatcgcgg acaccatatt ttgcagtacc
     4081 gatttgcata ataatgtcac aattaccttc tcggtgataa gccgaaagcc ccccttctcc
     4141 agtgtttaac cagcaacctg cttgggctgc tcccaaggat aaagcgcgaa ctgccggggc
     4201 tgaaatcgca ccataactca tcccactaat gttgataacc gattttgcct gaaaagggta
     4261 ctggcagtag ttttctccta taattacggg tggcgttggt aattgatctt cttccaaaat
     4321 tggaaatgca gcattgacga agataaaggc gccaggctct cgcaagtcat aagtagaacc
     4381 aaatccaacg ataccccctt tattttttgc actcttataa acccatgttc tagtagcacg
     4441 attaaagggc attt
//
LOCUS       DAOQLJ010000168         4437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_9457_length_4437_cov_6.422182, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000168 DAOQLJ010000000
VERSION     DAOQLJ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9457_length_4437_cov_6.422182"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /gene="mnmA"
                     /locus_tag="PK522_10030"
     CDS             <1..308
                     /gene="mnmA"
                     /locus_tag="PK522_10030"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635324.1"
                     /note="catalyzes a sulfuration reaction to synthesize
                     2-thiouridine at the U34 position of tRNAs; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNC42110.1"
                     /translation="VCRKDIEKNRLIVVQGHDHPALLSDSLQAMNLSWISGTAPHQNW
                     VYAAKTRYRQTDAPCSISRIDALSCQIDFAQPQWAVTPGQSVVIYESKVCLGGGIIN"
     gene            complement(305..922)
                     /locus_tag="PK522_10035"
     CDS             complement(305..922)
                     /locus_tag="PK522_10035"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HNC42111.1"
                     /translation="MKLIGSLTSPYVRKVRIVLAEKQINYDFEVDVPWNAQTNVSHHN
                     PLGKIPILIVDEKTSLYDSRVIAEYIDSLETQPGMIRLIPESGNTRWQVKRWEALADG
                     ICDAAATIFLERKRPEVQQSEEWIMRQQKKVDWGLIEAAKMLGEGDWCAGKSISLADV
                     ALGCALGYLSFRFPQIEWPRSYPNLNRLAEKLAQRASFIATAPRD"
     gene            1174..1629
                     /locus_tag="PK522_10040"
     CDS             1174..1629
                     /locus_tag="PK522_10040"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HNC42112.1"
                     /translation="MHKLTTYLKVVSRSRDHQRLFLNVNEIMRLQKTLSRKLPPQLAK
                     RCCIGSCSNGALVIKTSNSAVAAKLKQMVPSLLKEFENQIQSIRITVDTQDYKNQNDE
                     TKISKKPALNSVGIQALQQFEISLVHSPLKSAIHSLLSQYSINDRTENR"
     gene            1723..2628
                     /locus_tag="PK522_10045"
     CDS             1723..2628
                     /locus_tag="PK522_10045"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC42113.1"
                     /translation="MPVKRILSAVVLTVLFTQPNDRVLAEESRCQSTTHQGVGALGRI
                     EPRSRVIRISHNAGPQGARVEQLFINESDHVKSGDTLAILSDHSKKKAEVEAATARIK
                     VLEAQRAAEQALHTYNKKEHLRHQSLQPDAAVSISQIEAKKMAYEQSLANLNRFAAEI
                     HQAQSELKVSQAELDNTLIRAPIHGTILKIHRRSGERIGDSGLLEMADLSQLDIVAEV
                     YETDLHRVRVGQTGCISGAGFEHTYLAEVRELGYLVRKNDINDTDPLSDRDNRIIEVR
                     LTLEISAVENLQHQIFRQVKVKILP"
     gene            2655..3794
                     /gene="devC"
                     /locus_tag="PK522_10050"
     CDS             2655..3794
                     /gene="devC"
                     /locus_tag="PK522_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease DevC"
                     /protein_id="HNC42114.1"
                     /translation="MRLAWRQLIFDRTKLMVAICGVLFACVLVFMQLGFRDALYASAT
                     SAPVKLDGDLFLMHKQSEAMWRPIEFKRSELMRALGNPAVADVYPLYLGLAPFKNIET
                     KVKRTLMVYGFDPDSTSFRIEAINNKREALKLKDTVLFDEYSRPEFGPIGQLIEANRN
                     LTEIKDYKVAIIGLFRMGVSFAADGNVVTSDLNFMRIFPKRSLDNIDVGIIKLTPGSS
                     QTSVKGELEKLLDESINIFTYQELLQFEKNYWANMAPIGFIFGFGTIMGWVVGLVIVY
                     QILFTDIANHRNEFATLKAMGYSHGYFIRVVFASALLLAIFGFIPGLMLSLGLYHLAE
                     SQIFMPMPMTIAKATTVLMLILSMCFIAGLIAIRKLRDADPADMF"
     gene            3794..>4437
                     /locus_tag="PK522_10055"
     CDS             3794..>4437
                     /locus_tag="PK522_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC42115.1"
                     /translation="MQTAIDVQNLSFSFGSGALVQPVLKEITISIQQGEIVLITGPSG
                     SGKTTFLTIIGGLRQAFTGSVVVLGQQFIQSSEQVRVKVRQQIGFIFQQHNLLKSLTA
                     LQNVSMTLEMNNQLSEQQRQERAIAMLTAVGLGDRIHYKPDQLSGGQRQRVSIARALV
                     GQPRIVLADEPTASLDKQSGYEAVSILKKLAKESQTTILLVTHDYRISDVADRIL"
BASE COUNT         1323 a          978 c          912 g         1224 t
ORIGIN      
        1 ttgtttgccg caaagatatc gaaaaaaatc gattgattgt cgttcaagga catgatcacc
       61 cagcgctact ttctgattct ttgcaagcaa tgaatctatc ctggattagt ggcaccgcac
      121 cgcaccaaaa ttgggtctat gcggccaaaa cgcgctatcg ccaaaccgat gcgccctgtt
      181 caatttctcg aattgatgcg ctttcctgcc agatcgattt tgcgcaaccg caatgggcgg
      241 tcacccctgg gcaatcggtt gtaatctatg aaagcaaagt ttgcctgggt ggtggaataa
      301 tcaattaatc tctgggcgct gtcgcaataa atgacgctcg ctgcgctaat ttttccgcta
      361 atcgattaag attagggtaa gaccgaggcc actcaatctg tggaaaacga aaagataggt
      421 aaccgagtgc acaaccgagt gcaacatctg ccaatgaaat agactttcct gcacaccaat
      481 ctccctcccc taacatcttc gctgcttcaa ttaaacccca atcgaccttt ttttgctggc
      541 gcataatcca ttcttcgctc tgctgcactt cgggtctttt acgctccaga aaaatcgttg
      601 ctgctgcatc acaaattcca tctgcgaggg cttcccaccg ttttacctgc caacgcgtat
      661 tacctgattc cggaatcaat cgaatcatcc caggctgagt ttctaggcta tcgatgtatt
      721 ctgcgataac gcgtgagtca tacagagaag ttttctcgtc tacgataaga atgggaattt
      781 taccaagcgg attatgatga gaaacgtttg tttgagcatt ccaaggcaca tccacttcaa
      841 aatcgtagtt gatctgcttt tccgccaaaa cgatacgcac tttacgcaca taaggactgg
      901 ttagagaacc aattaatttc atcatcgtta tttatgctta tttccaataa tgtcagttta
      961 ctttacttag gctacccgta ccaagctata ctagcaggta tatttgagca tatattttta
     1021 tctcattatg attagcaagg attaatcaac agtgaatcat atgaacgttt gcaataaaac
     1081 gaagtaactg ggattattta tatttagaaa ttcgatttag ctgaaaaaca acctagcctt
     1141 gccaatcaat gattgggaaa accataacaa ttcatgcata agctgaccac ttatctcaaa
     1201 gtagttagtc gctcacgtga ccatcaacgc ttatttctca acgtcaatga aatcatgcga
     1261 ctacagaaaa cgctgtcgag aaagcttccg cctcaacttg caaaacgttg ctgcattggt
     1321 agttgttcaa acggtgcatt agtaatcaaa acctccaata gcgcagttgc agccaagtta
     1381 aagcaaatgg tgccatccct tctgaaggaa tttgagaatc aaattcaatc aattcgaatt
     1441 acagttgata cacaagatta taaaaatcaa aacgatgaaa caaaaatatc aaaaaaacct
     1501 gccttaaatt cagtgggaat acaagcactg caacaatttg aaatttcact cgtccactcc
     1561 cccctgaagt ctgcaattca ttctctatta agtcagtact caataaatga tcgtactgaa
     1621 aatcgctaaa gcatcaatcc atttgatcct gcaataaatt gatcagggaa acgatttgct
     1681 aaaatgagca cttgtcattt gtctaagaaa acttaaacaa gcgtgccagt taaaagaatt
     1741 ttgagtgctg tagtactcac cgttttgttt acgcagccca atgatcgagt attggcagag
     1801 gagtcgcgtt gccaatcaac aactcatcaa ggtgttggtg ctttagggcg cattgaacct
     1861 cgatctcgcg tcatcagaat ctcacacaat gcgggccctc aaggcgccag agttgagcaa
     1921 ctcttcatta atgaatccga tcacgttaag tcgggtgata cactagcaat tttgtcagac
     1981 cacagcaaga aaaaagctga agttgaagct gcgactgcac gcattaaagt gctggaagca
     2041 caacgggcag cagagcaagc tttacatacc tacaataaaa aagaacattt acgtcaccaa
     2101 tccctgcagc ccgatgctgc cgtgagtatc tctcagatcg aggcaaaaaa aatggcttat
     2161 gagcaatcat tagccaatct aaaccggttc gcggccgaga ttcaccaagc tcaatccgag
     2221 ctaaaagttt cacaagcgga attagacaac acgttaatcc gtgcaccgat tcatggaacc
     2281 attttaaaaa tccatcgcag atccggtgaa cgaatcggtg atagcggttt attggaaatg
     2341 gctgatttat cacagctcga tatcgtcgca gaagtctatg aaaccgattt acatcgagtc
     2401 agagtcggcc agaccggctg catcagtgga gcgggttttg agcatactta tctcgctgaa
     2461 gtcagagaat taggttatct ggttcgcaaa aatgatatca atgatacgga tcccctctca
     2521 gaccgtgaca accgtatcat cgaagttcgt ctaacgcttg aaataagtgc agttgaaaat
     2581 ttacaacatc agatttttag acaggtaaaa gtaaaaatcc tgccatgaat tacactaact
     2641 ggctttattt tccaatgcgt cttgcgtggc gccagttaat ttttgatcgc accaaactaa
     2701 tggttgcgat ctgtggtgta ttgtttgcct gtgtactggt tttcatgcag ttaggctttc
     2761 gagatgcact ctatgccagt gcaacatcag caccggtcaa actggatggc gatttgttct
     2821 taatgcataa acaaagcgaa gcgatgtggc gacccatcga atttaaacgt agcgagttaa
     2881 tgcgagcact aggtaatccc gctgtcgctg atgtatatcc gctttatcta ggattagcgc
     2941 ctttcaaaaa tatcgagact aaggtcaagc gcacactcat ggtatacggc tttgatccag
     3001 actcaacatc atttcgaatt gaagcgatca acaacaaacg agaagcgtta aaactaaaag
     3061 ataccgtact attcgacgaa tattcgcgcc ctgaatttgg cccaattggt cagttgatag
     3121 aagcgaatcg gaatttgact gaaatcaaag attataaagt agcgattatc ggcttgtttc
     3181 ggatgggtgt ttcttttgca gcggatggaa atgtagtcac cagcgatctc aattttatgc
     3241 gcatttttcc caaacgaagc ttggataata tcgatgtggg tattattaaa ttaacgccag
     3301 gttcatctca gacatctgta aagggtgaac ttgagaagct gctagatgaa tcgattaaca
     3361 ttttcacata ccaagagtta ttgcaatttg agaagaatta ctgggccaat atggcaccca
     3421 tcggttttat ttttggtttt ggaacgatta tgggatgggt tgtcggttta gtaatcgtct
     3481 atcaaattct ttttaccgat atcgcgaatc accgcaatga gtttgccacg ttaaaagcga
     3541 tgggatactc ccatggttat tttattcgag tggtatttgc ttccgcacta ttgcttgcta
     3601 tttttggatt tatacccggg ttaatgcttt ccttgggcct ttatcatctc gctgaatctc
     3661 aaattttcat gccgatgccc atgacgatag ccaaggctac cacagtattg atgctcattc
     3721 tttcaatgtg tttcatcgcc ggattaattg cgattcggaa actcagagat gccgatccgg
     3781 cagatatgtt ctaatgcaaa ctgccatcga tgttcagaat ttaagcttta gttttggaag
     3841 cggtgcttta gtacagcccg tattaaagga aataacaatc tcgatccaac aaggtgaaat
     3901 tgttttaatc acgggaccat ccggctcagg aaaaaccacc tttttgacta ttattggtgg
     3961 actcagacaa gctttcaccg gtagcgtagt agtactcggg cagcaattta tccaaagtag
     4021 tgaacaggtt agagttaaag ttcgacaaca aatcggtttt atctttcagc aacacaattt
     4081 acttaagtca ttaactgcgc tacaaaatgt cagcatgacg ttggaaatga ataaccagct
     4141 ctccgaacag caacgacaag agcgggcgat cgccatgctg actgcagttg gtcttggtga
     4201 cagaattcat tacaaacccg atcaactttc aggcgggcaa agacagcgtg tctcgatcgc
     4261 acgcgcgctc gtgggacaac ccaggatagt attagccgat gaaccgactg catcgctgga
     4321 taaacaaagt ggctatgaag cggttagtat ccttaagaaa ctagccaagg aatcacaaac
     4381 aacgatactc ctagtcaccc acgattaccg aatttcagat gtagcagatc gcattct
//
LOCUS       DAOQLJ010000169         4410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_9556_length_4410_cov_6.234443, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000169 DAOQLJ010000000
VERSION     DAOQLJ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9556_length_4410_cov_6.234443"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="PK522_10060"
     CDS             complement(<1..1201)
                     /locus_tag="PK522_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNC42116.1"
                     /translation="MEKNPEIAVYWSQPADSMMKSLGSNSQGLDPAIAADRLMHTGAN
                     RLKTNHFSGSTLQLFLNQFRSPLVLILIFAAIIALLVKDWLDALIVLMIVGMTAVLSF
                     IQEHRATRAVEKLRQKIATRSTVLRGGQTLVVPSDTIVPGDIVVLSAGNLIPADGMLL
                     EAKDFHVAQALLTGETFPVEKKPGISAPEASLVDRNNCIFMGTSVRSGYAKALIIQTG
                     LTTAYGKIAETLSLRPPETEFERGLRHFGQLLVRIMVIMVIAILAINIVLQHPTIETV
                     LFALALAVGLSPELLPAILTITLARGAKAMAAQGVIVKRLNAIENLGSMDVLCTDKTG
                     TLTKGVVQLGQALDSLGQTSKLVLQLAYVNSRLQTGIINLLDEAIIVAGEQSNITLTG
                     YQKLDEIP"
     gene            complement(1323..3035)
                     /locus_tag="PK522_10065"
     CDS             complement(1323..3035)
                     /locus_tag="PK522_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNC42117.1"
                     /translation="MPHSTILITTIAVSLGLALIMGMLANRLKLPVLVGYLVAGMMIG
                     PFTPGFVADLALSNQLAEIGVILLMFGVGLHFSLSDLLAVRKIALPGAIAQIIVATLL
                     GASVAMTWEWNLGASIVFGLALSVASTVVLIRALEQRGLMKSINGRIAVGWLIVEDLV
                     VVLALVLLPPLAGWLGGQGDDSSTSNNITDLLIVLAITFVKVSIFIGFMLIVGKRLFP
                     WLLWHVASTKSNELFILCVIAVAVGIAYGAAKLFDVSFALGAFFSGMVMRESSLSHRA
                     AQESLPLRDAFSVLFFVSVGMLFDPNILIEEPLHVLIVVAIILFGKSLAAFILVLAFR
                     YPLNTALTVSVSLSQIGELSFILASLGVSLELLPLEGYSLILAGAFISITLNHLVFET
                     VEPIQNWIRSRSKLARRLERTDDPLAELPTSVASTHVTGHVVIVGYGAVGKRVSETLA
                     SQGTHYVVVDQNREIVEQLRKHHAHAVAGDAADPAVLIQAHIARAGAMIITAADVLRI
                     QQMVETAKILNPQAEILISTHDPEAAARLEKENMGLVFLDEQVLAQNITQHLLKSLHE
                     STPH"
     gene            complement(3281..3472)
                     /locus_tag="PK522_10070"
     CDS             complement(3281..3472)
                     /locus_tag="PK522_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HNC42118.1"
                     /translation="MKIFAIVLLILILYSLGSGLYFMLKDDGSSTRMVKSLTIRIALS
                     LILFITLMISTYFNYIYQG"
     gene            3661..>4410
                     /gene="miaA"
                     /locus_tag="PK522_10075"
     CDS             3661..>4410
                     /gene="miaA"
                     /locus_tag="PK522_10075"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNC42119.1"
                     /translation="MPKFAPLNKLPPAIAILGPTASGKSNLALSITDHFPIEIISIDS
                     AQVYRYMDIGSAKPDPQILAKIPHHLINLIDPDQQYSAAQFRHDALLTMKEITLRGNI
                     PLLVGGTMLYFKALREGLSDLPPADQQIRKEIEASAAEKGWSTLHHLLQSVDPISAER
                     IQPNDSQRIQRALEIYYLTGKPMSQQMVSSQPASIPYRLISIALLPSDRKVLHQRIQH
                     RFETMLKMGLIEEVRSILSQFPLTLEMPSIRC"
BASE COUNT         1335 a         1021 c          894 g         1160 t
ORIGIN      
        1 agggaatctc atctaatttt tgataacccg ttagagtgat gttcgattgt tcacccgcta
       61 cgataatagc ttcgtccagt aaattgatga taccggtttg taacctggaa ttcacataag
      121 ctaattgcaa tactaatttg cttgtttgcc ccaagctatc gagcgcttga ccgagctgaa
      181 cgactccttt ggttaaagtg ccagttttat cggtacataa cacgtccata ctcccaaggt
      241 tctcgatggc attgagacgt ttgacgatga caccttgcgc tgccatcgct ttagcacctc
      301 gtgccaaggt aatggtaaga atggcaggca acaactcagg cgacagccct actgccaggg
      361 ccagcgcaaa taatactgtt tcaatcgtcg ggtgttgtaa gacgatattg atcgccagga
      421 ttgcgatgac cattatcacc atgatacgga ccagcaattg cccaaaatgc cgtaatcctc
      481 gctcaaactc cgtttcaggg ggacgtaatg aaagcgtttc agcaattttg ccataagcgg
      541 tcgtcaggcc tgtttgaatg atgagtgctt ttgcataccc acttcgcact gaagtaccca
      601 taaaaataca attattgcga tccactagac ttgcctctgg cgctgagatt cctggttttt
      661 tttctacagg gaatgtttct ccggttaata aggcctgtgc aacatgaaaa tccttcgctt
      721 caagtagcat tccatcagca ggaatcaaat tacctgcgga taacacaacg atatctcccg
      781 gtacgatggt atcagaggga acgacgaggg tttgtcctcc acggagcact gttgatctgg
      841 tcgcaatttt ctggcgtagt ttctcaacag cccgagttgc gcggtgttcc tgaataaaac
      901 tgagcactgc tgtcatcccg acaatcataa gcacgatcaa ggcatccaac caatctttga
      961 ccagcagcgc aatgattgca gcaaaaatta ggattaaaac caaagggctg cgaaattgat
     1021 ttaaaaaaag ctgtaaagtg cttccggaaa agtgattagt ttttaaccga tttgcgcccg
     1081 tgtgcattaa gcgatctgct gcgatggctg gatcgagacc ctgagagtta ctacccagcg
     1141 atttcatcat agaatcagcc ggttgactcc aataaaccgc aatctcagga ttcttttcca
     1201 tagcgttgat tattatcgat taggcaatag cattaacagc aaaacgccag aattttctgg
     1261 aaaattttgc tgccaagact aaagaaacat ttacttattc taagcttaaa atgtgctgaa
     1321 cctcaatgag gcgtagattc atgcaaactt ttcagtaaat gctgtgtaat gttttgtgca
     1381 agcacttgct catcgaggaa tactagcccc atattctctt tttccagcct tgcagcggct
     1441 tctggatcat gggtgctaat taaaatttca gcttgaggat ttaatatttt ggcggtttca
     1501 accatttgct gaatgcgcaa tacatcagca gcggtaatga tcattgcccc agctcgagca
     1561 atatgggctt gaattaatac cgctggatca gcggcatctc ctgctactgc atgcgcatga
     1621 tgttttctga gctgctccac aatttctcga ttctgatcga cgactacata atgcgtacct
     1681 tggctcgcaa gggtttcact cacacgctta cccactgcac catagccaac gatgaccaca
     1741 tgaccggtga catgcgttga tgcaaccgat gttggtaatt ctgctaacgg atcatcagtc
     1801 cgctctagtc ggcgggcaag cttagagcga gaacggatcc aattttggat cggttcaacg
     1861 gtttcaaaga ccagatgatt taaggtaatg gaaataaacg cgccagccaa tatcaagcta
     1921 tacccctcca gtggtaaaag ctctaatgaa accccgagtg aagcaagaat aaacgataat
     1981 tcaccgattt gagataagct caccgatacc gtcaaggccg tgttgagtgg atagcgaaat
     2041 gccagcacga gaataaaggc agctaacgat ttaccaaaca gaatgatagc aaccacaatc
     2101 agcacgtgca gcggttcttc aatcaaaata ttgggatcga acagcatacc cactgatacg
     2161 aagaacaata cggagaaagc atctcgcaaa ggtagcgatt cttgcgcagc acggtgactc
     2221 aagcttgatt cccgcatgac catgccagaa aagaaagcac ctagcgcaaa tgaaacatcg
     2281 aaaagcttag cagcaccgta agcaataccg accgcgacgg cgatgacgca tagaataaat
     2341 aattcattgg atttggtgct agcaacgtgc cacaacaacc aaggaaatag tcgtttaccg
     2401 acaatcaaca taaaaccaat aaatatcgat actttgacaa aagtaatcgc taatacaatc
     2461 aacaaatcgg ttatgttatt gctggtgctc gaatcatctc cctgccctcc caaccaaccc
     2521 gcaagggggg gaagcaatac taaagccagc actacaacta agtcttcgac gattagccag
     2581 ccgacagcaa ttcgaccatt aatcgatttc attaaaccac gttgctcgag tgcgcgaatc
     2641 aatacgaccg tacttgctac tgagagtgct agaccgaata cgatacttgc acctaaattc
     2701 cactcccaag tcatcgcaac actcgcacca agcaaagtcg ctacaatgat ttgagcaatt
     2761 gcacctggta gtgctatttt tctaactgct aatagatcac tgagtgaaaa atgcaaaccc
     2821 acgccaaaca tcagcaaaat aacgccaatt tctgctaatt gattagataa agcaagatcg
     2881 gctacaaatc ccggagtaaa gggaccgatc atcatgccag caaccaaata accgacgagt
     2941 accggaagct ttaaacgatt tgcgagcata cccatgatca aggcaagacc cagactaaca
     3001 gcgatcgtcg taattaaaat tgtgctatgg ggcatagacg agaaggagtt ttatggttga
     3061 tttaactgac tattttgaat cgactaagct ttttgttgtt tttaagatcg aatgcgacag
     3121 ctttgtctgg tccaacaggt ttgttattct tagtaagccc tagaatagct tcatcattac
     3181 tgagtctaat cttctaggcc aggagagtcg ctagcaaacg ccagtcaatg atcgcttcca
     3241 ctaatatgta aacattggaa gcgctttggc tttctgcttt tcatccttga taaatataat
     3301 tgaaatacgt tgagatcatg agtgtaataa acagaatgag agatagtgcg atccggatgg
     3361 tcagtgattt aaccatgcga gtactactcc catcatcttt taacatgaaa tacaacccag
     3421 agccaaggct ataaagaatg agtatcaata atactatcgc aaatattttc actacaattt
     3481 cctataaaaa tggaatgata tctcactgat gccattcatt aactgcggaa tcacaatcga
     3541 acataatcat cagttttttc ctattgtaac tgataacgat caattatgat tgttacagtc
     3601 cataacaact taccccaatt tagttatgat cacgttactc aagaaaaatt gcattaactg
     3661 atgcctaaat tcgctccatt aaataaatta cctccggcca ttgctatctt gggacctacc
     3721 gcaagtggta aatctaacct agccctatcg attaccgatc atttcccaat agagattatt
     3781 agtatcgact ctgcgcaagt ctatcgctat atggatattg gtagcgcaaa acccgaccca
     3841 caaatcttgg ctaaaattcc tcatcatctg atcaatttaa ttgatccaga ccagcaatat
     3901 tctgctgcgc aatttcgtca tgatgcactt ctcacaatga aagaaataac gctaagggga
     3961 aatattccat tgctagtcgg cggtaccatg ctttatttca aagcgttgag agaaggcctg
     4021 tctgatcttc caccggcaga tcagcaaatt cgtaaggaaa tcgaagcaag tgctgcggaa
     4081 aaaggctggt ctaccctgca tcacttgctg cagtcggttg atcctatcag cgctgaacgc
     4141 attcaaccca atgatagtca acgaatacag cgcgcgctgg aaatttatta cttgactggg
     4201 aaacccatgt cacaacagat ggtgtcttct cagcctgcat cgattcctta tcgattgatt
     4261 tccattgcat tattgccatc ggatcgcaaa gtattacacc agcgaattca gcatcgtttt
     4321 gaaacgatgt tgaaaatggg attaatcgaa gaagttcgat cgattctgag tcaatttcca
     4381 ctgacgctag aaatgccatc aatacgctgt
//
LOCUS       DAOQLJ010000170         4403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_9578_length_4403_cov_6.732981, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000170 DAOQLJ010000000
VERSION     DAOQLJ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9578_length_4403_cov_6.732981"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /gene="cgtA"
                     /locus_tag="PK522_10080"
     CDS             complement(<1..569)
                     /gene="cgtA"
                     /locus_tag="PK522_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HNC42120.1"
                     /translation="MKFIDEATILVSAGNGGDGVASFRREKFIPKGGPDGGDGGHGGS
                     IYAIADHNINTLIEYRYTPAFRAKKGENGRGSDCYGKGAEDIVLRMPVGTVITNAETG
                     EVIADLKHHLQKELLAKGGKGGLGNLHFKSSTNRAPRQFTRGGIGDEFQLKLELKVLA
                     DVGLLGMPNAGKSTLIRAISAARPKVADYP"
     gene            complement(710..967)
                     /gene="rpmA"
                     /locus_tag="PK522_10085"
     CDS             complement(710..967)
                     /gene="rpmA"
                     /locus_tag="PK522_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNC42121.1"
                     /translation="MAHKKAGGSSRNGRDSHSKRLGVKRYGGELIKAGNIIVRQRGTQ
                     FHPGDNVGMGKDHTLFAKIEGKVLFSMKSSFNRRTVSIVPV"
     gene            complement(983..1294)
                     /gene="rplU"
                     /locus_tag="PK522_10090"
     CDS             complement(983..1294)
                     /gene="rplU"
                     /locus_tag="PK522_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNC42122.1"
                     /translation="MYAVIKTGGKQYRVEVGNKLKIEQIKADAGSDIVLDQVLMVANG
                     DDVTVGKPYLEKAKVNASVLCHGRHDKIRIFKMRRRKHYQKHQGHRQNYTEIQITNIS
                     A"
     gene            1441..2409
                     /gene="ispB"
                     /locus_tag="PK522_10095"
     CDS             1441..2409
                     /gene="ispB"
                     /locus_tag="PK522_10095"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HNC42123.1"
                     /translation="MSIDSVRNFIAQDMSAVDEVIRQKLYSQVALIRQISEYIINNGG
                     KRLRPALVILSAGVFGYSGKYHHQLAAVVEFIHTATLLHDDVVDESELRRNNATANAL
                     FGNAASVLVGDFLYSRAFQMMVEVNSMRVMQVLADATNVIAEGEVLQLLNCRDPNVEE
                     DNYLRVIRYKTAKLFEAATRLGAILGNATEEEENAMAVYGMHLGTAFQLIDDLLDYSG
                     DHQDTGKNLGDDLAEGKPTLPLIYAMRTGSAQQANIIRKAIETGGESGFDSILTIIEE
                     TGALNYAKQRAYQEVEIAAKAISLLKSSHYKDCLLQLAEFAVTRNH"
     gene            complement(2440..3876)
                     /locus_tag="PK522_10100"
     CDS             complement(2440..3876)
                     /locus_tag="PK522_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNC42124.1"
                     /translation="MQNSIGEQLVEDGLLTPSQLAQAYTWQQHNGRQLAYAITYLGFV
                     TQAQLTHYLNLELPVPQKIEDTGLSEALLTDLLLKSAYLESGIFSIQSITSSLSLPFS
                     VVNQLIELIRSDHLITVRGAASYTSSQPLELTQRGRERAEAALQISSYVGAAPVPIEV
                     YTHILNKQSVRLIELDREWVYSSLSHLVMSDQLINQLGPAFASGRSVFLYGQPGVGKT
                     SIAEALGNSLPGEVYIPQALEVGGQIIRFYDPVTHVSIESDQTEHVSQLDLSKKLTYD
                     PRWKKCRRPVIMVGGELTLDMLELKYDAVSKFYEAPIHMKASNGVFILDDFGRQRVDP
                     RQLLNRWIIPLEKGVDFPSLHTGLKFKIPFDQITVFSTNLKPTDLVDEAFLRRIRHKI
                     QIPSQTEGEYKEILRRACHTYGVHYDSSVVEYLLNNYYRKQNRTLAGSHPRDLLEHII
                     EYAAFLKQTPEFTIETVNAAAANYFVDL"
     gene            4148..4282
                     /locus_tag="PK522_10105"
     CDS             4148..4282
                     /locus_tag="PK522_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42125.1"
                     /translation="MSWLLCLLCNICFSDGVQNYAHARAQHAEHDHLVAPQTIDESHS
                     "
BASE COUNT         1267 a          975 c          839 g         1322 t
ORIGIN      
        1 gggtagtcag caacttttgg tcgagctgcc gatatagcac ggatcaaagt agatttaccc
       61 gcatttggca ttcccaataa acctacatcg gcaagtacct tcaactcaag cttaagctga
      121 aattcgtccc caattccccc tcgggtgaat tgtctaggtg cacgattggt acttgattta
      181 aaatgcaagt taccgagccc tcctttgccc cctttcgcca ataattcttt ttgcaggtga
      241 tgttttaaat ctgctatgac ttctcccgtc tcggcatttg tgatcacagt tccaacgggc
      301 atgcgcaaca caatgtcttc agcccctttt ccataacaat ccgaaccacg cccattttca
      361 cccttcttgg cacgaaaagc aggtgtgtaa cgatattcaa tcaaagtgtt tatattatga
      421 tctgcaatgg catagatact tccgccatga ccgccatccc ccccatcagg accccctttc
      481 ggaataaatt tttcacgacg aaagctcgca acaccatccc ctccattacc ggcggaaacc
      541 aatatagttg cctcatcaat aaacttcata aactatcgta gtagaaaaat taatgcacaa
      601 gaaaaacagg aaacaaattt gcaaatttca gtaacaacta attataaaat caagttacaa
      661 ataaaaaagc cccatcactt gaatggggct ttttattcat ttctatcagt taaacgggaa
      721 ctatcgatac agtcctacgg ttaaatgaac ttttcatgct aaagagcact ttcccttcaa
      781 tttttgcgaa taatgtatgg tctttaccca ttcccacatt atcaccagga tgaaattgcg
      841 ttccacgctg acgaacgata atatttcccg ctttaattag ctcgccgcca tagcgcttaa
      901 cccctaggcg cttggagtgt gaatcgcgtc cgtttcttga gcttccgccc gctttcttat
      961 gtgccatcta aattctccta tcttatgctg aaatattagt aatttgaatt tcagtataat
     1021 tttgccgatg cccttggtgc ttttgatagt gcttgcgtcg ccgcatttta aagatgcgaa
     1081 tcttgtcatg gcgaccatga catagaacag aagcatttac cttcgctttt tcaaggtaag
     1141 gtttaccaac cgtgacatca tctccatttg caaccatgag cacttgatca agcacgatat
     1201 cacttccggc atctgcttta atttgctcga ttttaagctt attaccgact tcaacgcggt
     1261 attgcttacc gcctgtttta atcactgcat acatatcgtt catacccttt ccactaattg
     1321 gcatgaatta taccttccta taagcgtttt gtgtgataaa aaaacaatct taaacgaatg
     1381 atccgacttg acactctagt aaagacattc ctaccatagt gttttctaca aggggaagtt
     1441 gtgtctatag attctgtaag aaattttatc gctcaagata tgagcgcggt ggatgaagta
     1501 atacgccaaa aactctattc tcaagttgcg ctgattcgac aaatcagtga gtatattatt
     1561 aacaatggcg gtaaacgttt acgcccagca ttagtcatct tatctgcggg cgtattcggt
     1621 tatagtggaa aataccatca tcagcttgct gccgttgttg aattcattca taccgcaact
     1681 ttattgcacg acgatgttgt tgatgaatcg gaactgagac gaaacaatgc aacagccaat
     1741 gcattatttg gtaatgcagc cagcgtattg gttggtgatt tcctctattc acgtgctttc
     1801 caaatgatgg tagaagtcaa cagtatgcgt gttatgcaag ttcttgctga tgcgaccaat
     1861 gtcattgccg aaggtgaggt attacaactg ctcaattgcc gagatcccaa tgtagaagaa
     1921 gataattacc tacgggttat tcgctataaa acagccaaac tttttgaggc agcgacacgc
     1981 ttaggtgcaa ttcttggtaa tgctacggaa gaagaagaaa atgccatggc cgtttatggg
     2041 atgcatctcg gtacggcatt ccaactcatc gatgacttgc tcgattattc gggggatcat
     2101 caagatacgg gcaaaaattt aggagatgat ctcgctgagg gtaagccgac attacccctc
     2161 atttatgcga tgcgaactgg ctcagctcaa caagcaaaca tcattcgtaa agcaattgaa
     2221 actgggggag aaagtggatt tgattctatc ctaacgatta tcgaagaaac gggggcactt
     2281 aactacgcta aacaacgtgc ttaccaggaa gtcgaaattg cggccaaagc gatatctctg
     2341 ttaaaaagct ctcattataa agattgccta ttacagctcg ctgagtttgc ggtaactcgc
     2401 aaccattaga aacggttact gcaatcccag ttcgattatt tatagatcga caaagtaatt
     2461 agctgcagct gcattaactg tttcaatggt aaattctggt gtttgtttca agaatgcagc
     2521 gtattcaata atatgttcga gcaaatcacg cggatggctt ccggctagcg tacgattttg
     2581 cttacgataa taattattca ataggtactc caccacgcta ctatcgtaat gaacgccata
     2641 agtatggcac gctcgacgca atatctcttt atattcccct tccgtttgac tgggtatctg
     2701 aatcttatgg cgaattcgac ggagaaatgc ttcatccacc aaatctgttg gtttgagatt
     2761 cgttgaaaaa acagtaattt gatcaaaagg gattttaaat ttcaatccgg tatgtagcga
     2821 tggaaaatca accccttttt ccagcggaat aatccagcgg tttaataact gacggggatc
     2881 gacacgctgc cgaccgaagt catcgaggat gaaaacacca ttactcgcct tcatatgaat
     2941 cggtgcttcg taaaatttac tcaccgcatc gtattttaat tcaagcatat ctaaggtgag
     3001 ttcacctccc accataatga ctggtctacg acactttttc cagcgcggat cataagttaa
     3061 cttcttactc aaatctaact gtgacacgtg ttccgtttga tcagattcga tactgacatg
     3121 agtcacaggg tcataaaatc gtatgatctg cccgcctact tctagcgcct gaggaatata
     3181 aacctcaccg ggtaaactat tacccaatgc ctccgcaata ctggtttttc ctacacccgg
     3241 ttgcccgtat aaaaatacag agcgtcctga tgcaaaagcg ggacccaatt gattgattaa
     3301 ctgatcgctc attaccaaat gtgacaatga tgaataaacc cactcacgat ctaattcaat
     3361 taggcgcact gactgtttat tcaatatatg cgtgtatact tcgattggca caggtgccgc
     3421 acccacataa gaactaattt gcaaggctgc ttcagctctt tccctgccgc gttgggtcaa
     3481 ttcgagcggt tgtgaactcg tatagctcgc tgctccccgt acagtaatta aatgatcgga
     3541 tcgaatcagt tcgatcagct gattaactac gctaaaagga agcgataaag aacttgtgat
     3601 actttgtata gaaaaaatgc ctgattctaa gtaagcggat tttaacaaca ggtccgtcaa
     3661 aagagcctct gagagacccg tatcctctat tttttgcggt accggtaatt ctaaattcag
     3721 atagtgagtt aattgtgcct gagttacaaa acccaaatac gtgattgcat aagccaattg
     3781 ccttccatta tgctgttgcc acgtataagc ctgcgctagc tgtgaaggcg ttagtaatcc
     3841 atcctcaacc aactgttcac caattgaatt ttgcattgtt aaatatctat ccttaaaaat
     3901 ttatttgctt acagataaaa cttggttcac ctaaagatca ttcgtaacgc tcttacttca
     3961 aatgctgcta gtccattggc acggatacat agagtaagaa tatacgaatg aatctacact
     4021 gttgtttaat tctgtaaaat aaggaatagg ttggtaatta gggagcttct ttgattcttg
     4081 attagaagaa actcaggttt agagctacta ttcaatcttg gatatttcag taagcttctt
     4141 caaatctatg agttggttac tttgcttgct ctgcaatatc tgctttagtg atggtgttca
     4201 aaattatgct catgcccggg cacagcatgc tgagcatgat cacttagttg cgcctcaaac
     4261 cattgatgaa tcgcattctt aagtttttgg atttctgtag aatatgtaat ctcagcaccg
     4321 tctggtaatt ctttataact tatttttatt aatgcctatg aactattctc agtgtttttc
     4381 atcttatata gcaagatcaa tag
//
LOCUS       DAOQLJ010000171         4379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_9661_length_4379_cov_5.868409, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000171 DAOQLJ010000000
VERSION     DAOQLJ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9661_length_4379_cov_5.868409"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..143
                     /locus_tag="PK522_10110"
     CDS             15..143
                     /locus_tag="PK522_10110"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42126.1"
                     /translation="MASSQLEIPDVSAAKKTLENLIQKYPNSEAAGKAKKRLTSLK"
     gene            189..830
                     /gene="queE"
                     /locus_tag="PK522_10115"
     CDS             189..830
                     /gene="queE"
                     /locus_tag="PK522_10115"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNC42127.1"
                     /translation="MEKLRVSEIFYSLQGETTRMGLPTVFIRLTGCPLRCVYCDTQYA
                     FTGGKMMSIPEILDKVSNSKARYVTVTGGEPLAQKACLSLLTALCDAHYSVSIETSGA
                     FDISEIDPRVSRIVDIKTPGSGEVSKNLFENLALISSKDEIKFVLCDREDYQWALQIM
                     NDYQLAQVCPILFSPTYGQLAADLLAKWILEDHAPVRLQLQLHKILWGEVAGR"
     gene            870..1562
                     /gene="queC"
                     /locus_tag="PK522_10120"
     CDS             870..1562
                     /gene="queC"
                     /locus_tag="PK522_10120"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNC42128.1"
                     /translation="MKKAVVLVSGGLDSATTLAVARDQHFDCYALSIDYGQRSRSELD
                     AALNVGKMIGVTDHQVISVDLRNLGRSALTDQSIDVPTTGMQVGIPVTYVPARNTIML
                     SLALAWAESLASQAIFIGVNAIDYSGYPDCRPDYIEAFERMANLATKAAYEGVKLKIH
                     APLIDLSKREIILLGTRLNVDYSRTVSCYQADEHGLACGVCDACRLRKMGFSAAGISD
                     PTRYSPIYREPQ"
     gene            complement(1577..1948)
                     /locus_tag="PK522_10125"
     CDS             complement(1577..1948)
                     /locus_tag="PK522_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNC42129.1"
                     /translation="MDVIFLQNFRTKAMIGLYPWEKKVAQLIELNLEIALPTRSASAS
                     DDIKDTIDYSLIADRIKHTLSDKHFMLLEALAEHIAQLILAEFKSPQVKVSVAKLGII
                     KGIEKLGVSIVREFDSHQHEN"
     gene            2039..2653
                     /gene="plsY"
                     /locus_tag="PK522_10130"
     CDS             2039..2653
                     /gene="plsY"
                     /locus_tag="PK522_10130"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNC42130.1"
                     /translation="MATIILLLMAYLLGSIPFAMVASKLFDLPDPRTYGSKNPGATNV
                     LRSGKIAAAVVTLLGDAGKGWLAVELTRVYVSMEGGGYELISGAALLVFLGHLFPIFL
                     NFKGGKGVATAAGVLLGFNVLLGSLVILTWVIVAAISRLSSLSALTAAVLAPIYSYLI
                     FNWDTPVWAVIIISLLLMLRHQANIINLIEGKETRIGKKATDKT"
     gene            complement(2936..3238)
                     /locus_tag="PK522_10135"
     CDS             complement(2936..3238)
                     /locus_tag="PK522_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42131.1"
                     /translation="MKNPELKLNINGSYERARLDKVRADKEQLELDLARRNLVLRSDV
                     HNAAFEAGRKLRDTLHSVCKQSAPNLVNIEDPAKIEMYLRDEVDRALNEFIKSCEL"
     gene            complement(3467..4240)
                     /locus_tag="PK522_10140"
     CDS             complement(3467..4240)
                     /locus_tag="PK522_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HNC42132.1"
                     /translation="MAGSHLFRMGTVKGKNGVLIALKHNKRALQAERGASANINPFRT
                     PLNYSLTKPDNAEAIDRHAKVLMVQAGIDQPRKNQVMAVEAIFSLPIDQHQQDTRPFF
                     QCCMDWIVRNIPGVLLSFDVHLDETAPHAHALILPLVDNKMQGNKIMGGKGNLMRLIN
                     LFHAEVARYYGLSRNETKRLTNKAKQSLERQVLAKLEIDPVMKSLIWSCVRDAIHDNP
                     LPFAQLLGIEQKLAKYTGRSFVQIMTSKGKGEEIELNTI"
BASE COUNT         1212 a          857 c          952 g         1358 t
ORIGIN      
        1 acgcgcttct caatattgcc agtagtcaat tggaaattcc tgacgtatcg gctgctaaaa
       61 aaaccttaga aaaccttatc caaaaatatc ccaatagtga ggctgctggt aaagcaaaaa
      121 agcgcctaac tagcctgaaa taatttttat caagaaggaa gtttttacag cttcttgctt
      181 ttttctccat ggaaaagttg cgagttagtg agatttttta ctccctgcag ggtgaaacaa
      241 cgcgtatggg tttgccgacg gtattcattc gtttaactgg ctgtccttta cgctgtgttt
      301 actgtgatac acagtatgca tttaccgggg gaaagatgat gtcgattccg gagatattgg
      361 ataaggtttc aaatagtaag gcacgctatg taacagttac cggtggtgaa ccgttggcac
      421 aaaaagcctg tttatcttta cttactgcat tatgtgatgc ccattattct gtctccattg
      481 agaccagtgg ggcattcgat attagtgaaa ttgatccgcg tgtctcccgc attgtcgata
      541 ttaaaacgcc tggatcgggc gaagtatcaa agaatctttt tgagaaccta gcgctgatat
      601 cgagtaaaga cgaaattaaa tttgtattgt gcgataggga agattatcaa tgggcgctac
      661 agataatgaa tgactatcaa ttagctcaag tatgccccat cttattttcc cctacttatg
      721 gtcagctagc ggctgattta ttagcaaaat ggatcttgga agatcatgca ccggttcgat
      781 tgcagctcca attacataaa attttgtggg gagaggtagc gggtcgatag ccgaatgggt
      841 taattttctg gctactttgt tttattgaga tgaaaaaagc ggttgtattg gtatcgggag
      901 gactcgattc tgcaacgact cttgcggttg ctcgtgatca acatttcgat tgttatgcac
      961 tcagtattga ttacggacag agaagtcgct ctgaactcga tgcagcgtta aatgttggga
     1021 aaatgatcgg tgtcacggat catcaagtca ttagcgttga tctgcgaaac ttgggaagat
     1081 cagcactgac ggatcaatcg atcgatgttc ccacgacagg aatgcaagtg ggtattccag
     1141 tgacttatgt gccagcacgc aatacgatta tgctttcgtt agcgttagct tgggctgaaa
     1201 gcctagcaag ccaagctatt tttattggcg tcaatgcaat cgattattct ggctatccgg
     1261 actgtaggcc ggactatatc gaggcttttg agcgtatggc aaatctagcc actaaagctg
     1321 catatgaggg ggtaaaactc aagattcatg caccactgat tgatttatcg aagcgcgaaa
     1381 ttattttgtt gggtactcgg ttgaatgttg attatagtcg tacagtttcc tgttaccaag
     1441 cggatgagca tggattggca tgcggtgtat gtgatgcgtg ccggttgcgt aagatgggtt
     1501 tttcagcagc tggcatttct gatccaactc gatattcacc aatctatcga gagccccagt
     1561 aatcaaggaa aagttatcaa ttctcatgct gatgagaatc gaactcacgc acgatgctga
     1621 caccgagctt ttctatacct ttaataattc caagttttgc aacacttact ttaacttgag
     1681 gtgatttaaa ctcggccaag ataagttggg cgatatgctc tgccaaagct tcgagtagca
     1741 tgaaatgctt gtcagacaaa gtgtgcttga ttctgtcagc aataagggaa taatctatcg
     1801 tatctttgat atcatcactg gcactcgcgc tacgtgtggg aagtgcaatt tctaagttaa
     1861 gttcgatgag ttgcgcgact tttttttccc aaggataaag accgatcatc gctttggttc
     1921 gaaaattttg taagaaaatg acatccatgg gagtaattta ctgcattata tttacaatag
     1981 tgctagattt ttttgttaac gattctaatc gaattagaga ggcgaagcta ttttggatat
     2041 agcaacgatc attttattac tgatggccta tttacttgga tcgataccat ttgcgatggt
     2101 agccagtaaa ttattcgact tacctgaccc aagaacctac ggatcaaaaa acccaggtgc
     2161 aaccaatgtg ttacgaagtg gcaaaattgc tgctgcagtt gtaaccctat tgggggatgc
     2221 cggtaaaggc tggttagcag ttgagctaac tcgagtttat gtttcgatgg aaggaggcgg
     2281 ttacgaatta attagcggtg ctgcattatt ggtttttctc gggcatctat tccccatttt
     2341 tctcaatttt aaaggcggga aaggtgtagc gacagctgca ggcgtattac ttgggtttaa
     2401 tgtgttatta ggttcgcttg tgattttgac ttgggttatc gttgctgcta tctcaaggct
     2461 atcgtcccta tctgcgctca ctgctgcggt gcttgccccg atttatagtt atttaatttt
     2521 taattgggat acccctgttt gggcggttat catcatttcg ttactgctaa tgctgcgtca
     2581 tcaagcaaat atcattaatt tgattgaagg aaaggaaacg cgtattggta aaaaagcaac
     2641 agacaagact taagtatgct gaatttcaaa gaatccaagt ttcgtaaaat aaagacacga
     2701 aatgggggac atgacgaaac gattatcgcc gacgaagtat aaccaagaat ccagtgagcg
     2761 actagttaag tttttttaaa ttaaatcgat tggcgcgaga ggggtaaccg caaatatata
     2821 ataagatatt gatttaaata ttattaatat ttattgattt tataagtacc gccaaaaatg
     2881 ccgtcaattt attttgctgg gctggttcct agctgtcaac atgcttggtt tggtgttaca
     2941 gctcgcatga tttaataaac tcattaagcg cgcgatctac ttcatcacgt agatacattt
     3001 caatctttgc tggatcttca atattcacaa gattaggtgc agattgtttg caaacagagt
     3061 gcaatgtgtc gcgaagcttg cgacctgctt cgaatgctgc attgtgtacg tcagaacgca
     3121 ataccaaatt acgtcgcgca aggtctaatt ctagctgctc tttatcagca cgaaccttgt
     3181 caagtctagc cctttcgtaa gagccattaa tattaagttt aagttcagga tttttcatgt
     3241 gtatagaatt tgaatttcta gatttaagat gaggccttca ttacccccac ttaaattatc
     3301 aagagaagaa cctacttttt atttagttac tcacttacac gcctattggt attaaaagtt
     3361 actgattcgg taatcgtgac cggtttagta acctctacca tttagaatgg tcagaaaacg
     3421 aaacgctata cacaggatag cgttttatgt tttaaaggca gaaacgctat atagtgttca
     3481 attcaatttc ctctcctttg cctttggaag tcataatctg tacgaatgat ctcccggtat
     3541 atttggcaag cttttgttca attcccaata attgcgcaaa gggtagcgga ttatcatgaa
     3601 tcgcatcgcg cacacacgac cagataagtg atttcatcac tggatcaatt tctaatttag
     3661 ccagaacctg tctttcaagt gattgcttag ctttatttgt gagtcgttta gtttcgtttc
     3721 tgcttagtcc gtaatacctg gcaacttcgg catgaaacag attaatcaaa cgcattagat
     3781 tacctttgcc gcccatgatt ttgtttccct gcattttgtt gtctaccaag ggtaaaatta
     3841 aagcatgtgc atggggtgcg gtttcatcca ggtgaacatc gaatgacaac aacacccccg
     3901 gaatatttct tactatccaa tccatgcaac attggaagaa tggccgagtg tcttgttggt
     3961 gttgatcaat gggtagacta aagatggctt ctactgccat cacttggttt ttacgcggct
     4021 gatctatgcc agcctgcacc atcaatactt ttgcatggcg atcaatcgct tctgcgttat
     4081 ccggtttagt cagtgagtaa ttcaatggtg ttctaaatgg attaatattc gcgctagcac
     4141 ctcgttcagc ttgtagcgct cgcttattgt gcttcaaagc gatcagaacg ccatttttac
     4201 ctttcaccgt gcccatgcgg aataggtgac taccggccat cgtttaacca tcctccagcc
     4261 gcaacagata ccgataaact gcccacaatt cgatcagact cattgaatca atatcagcac
     4321 caataccaag atgagaaaac cgtattaaca gcatttcaag ttgttccaac caagtatca
//
LOCUS       DAOQLJ010000172         4373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_9685_length_4373_cov_6.301760, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000172 DAOQLJ010000000
VERSION     DAOQLJ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9685_length_4373_cov_6.301760"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /locus_tag="PK522_10145"
     CDS             complement(<1..1669)
                     /locus_tag="PK522_10145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HNC42133.1"
                     /translation="MNKIYLRACWLVVLIAFPFLVNAAGLGKLTLGSSLGQPLRAEIE
                     LVSVKNDEIPSLVARVASPEAFRQAGVALAPYHSSLVISVEKRANGQPYIQISSPQSI
                     NEPFLNLLIELNWSSGRLLREYTVLLDPVDTNIAEPASPVVSPVTEAAPQIAGTPTTS
                     AQNNQSKQKPKQPTPKARTSSTAGDTYGPVVKGDTLTRIARQVTPDGVDLNQMLVALY
                     RANRSAFFEKNMNLLKVGTILRIPDQNEVSAIARKEANREVKTQIADWQNYRQRVATA
                     GTTSTNQLPQRQELKQTATGKITATVEEPKLKKEDAPTEVLILSKGEQLGDGKNNVGK
                     PDSQPSEPSQDYVRMMEEDAIAKDRALTEANERVSMLEHNIERLQRLLEIKNAGMADI
                     QKQAEQTQTNPTPPTQLADDKEIASDKPADDIASLQEPAQTEELSLQSDSITVPSLPA
                     PSILQNTPIPAPTPPEPETDFVEQMMGFVSELVDFTTENIEIVGGILITLLTGWLGIS
                     YVRRKKSQANNIDEDEINFAEEKPIRNEEEANTSFSSTFASEAAINQF"
     gene            complement(1826..2938)
                     /gene="asd"
                     /locus_tag="PK522_10150"
     CDS             complement(1826..2938)
                     /gene="asd"
                     /locus_tag="PK522_10150"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNC42134.1"
                     /translation="MKQVGFVGWRGMVGSVLMQRMREENDFSLVEPIFFSTSQKNGKG
                     PDIGQEIPPLKDAHDLSELKKMDIIITCQGGDYTNEVFGKLREAGWKGYWIDAASTLR
                     MKDDAVIILDPVNMPVIKQALHEGTKNYIGGNCTVSLMLMGIGGLFENNMVEWMSAMT
                     YQAASGAGAQNMRELLQQMGEAHHVAEELLNNPASSILDIDREVSEKLRDKKFPVENF
                     GVPLAGSLIPWIDREVAQGQSREEWKGQAETNKILGHQSKPVPVDGLCVRIGAMRCHS
                     QALTIKLKQDIPLNEIEDVIAQSNDWVRVIPNEREKTTKELTPVAVTGKLDVHVGRIR
                     KLSMGGEYLTAFTVGDQLLWGAAEPLRRMLRILVEN"
     gene            complement(3071..4132)
                     /gene="leuB"
                     /locus_tag="PK522_10155"
     CDS             complement(3071..4132)
                     /gene="leuB"
                     /locus_tag="PK522_10155"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNC42135.1"
                     /translation="MKIAVLPGDGIGPEITQQAEKILMALASDGLKIELEHGFIGGSA
                     VDKYNDPFPEATHRLAEEADAILLGAVGGPKYDQLPREKRPEQGLLAIRKALNFFANL
                     RPAQLYPELANASTLKPEIVANLDILIVRELTGDIYFGTPRGIEVRDGQRVGFNTMIY
                     TESEIRRIAHVAFQAARKRSSKLCSVDKMNVLECTQLWRNVVEEVAKEYPDVALSHML
                     VDNAAMQLIRNPKQFDVIVTGNMFGDILSDEASMLTGSIGMLPSASLNEFNKGLYEPI
                     HGSAPDIAGKNLANPLATLLSVSMMLRYTFNQDMAASRIEDAVKKVLASGYRTVDIFE
                     QGMIKVGTAEMGNAVLANL"
     gene            complement(4160..>4373)
                     /locus_tag="PK522_10160"
     CDS             complement(4160..>4373)
                     /locus_tag="PK522_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNC42136.1"
                     /translation="QATEGYRLAINLEAQTVTTPTKECYHFDVDSFRKHCLINGLDEI
                     GLTLQHTDKIKLFEQKRQSEQPWLFI"
BASE COUNT         1171 a          963 c          861 g         1378 t
ORIGIN      
        1 caaattgatt aatggcagct tcggaagcga aagtactgga aaacgatgta ttcgcttctt
       61 cttcgtttct aattggtttt tcttcagcaa aattgatttc atcttcatca atattattcg
      121 cttgcgattt cttgcgtcga acatacgaaa ttcctaacca tcccgttaat aacgtgataa
      181 gtatgccacc cacgatttcg atgttctctg ttgtaaaatc aaccagttct gagacaaacc
      241 ccatcatttg ttcaacgaaa tctgtttcag gttcaggcgg tgtaggtgcc ggtatagggg
      301 tattttgcaa tatcgaagga gctggaagac ttggaacagt gattgaatct gattgtagtg
      361 acagttcttc ggtttgagca ggttcttgca aggacgctat atcatcagca ggtttatcag
      421 aagcgatttc tttgtcatca gctagctgag tcggtggcgt aggattagtc tgcgtttgct
      481 ctgcttgttt ttgtatatct gccattccag cattcttgat ttcaagcaac cgttgtaaac
      541 gctctatatt gtgctcaagc attgagacgc gttcatttgc ttcagttaat gctctatctt
      601 tggcaattgc atcctcttcc atcattcgca cataatcttg agatggttca gatggctgag
      661 aatctggttt acctacatta tttttgccgt caccaagttg ttccccctta gataaaatca
      721 aaacttcagt aggggcatct tctttcttca acttgggttc ttcaaccgtt gctgtaatct
      781 tacccgttgc tgtctgcttt aattcttgcc gctgaggtaa ttggttcgtt gaagtagttc
      841 ctgccgttgc aactctttgt cggtaatttt gccaatcagc gatttgagtt ttaacttcac
      901 gatttgcttc tttgcgcgcg attgcagaaa cctcattctg atcgggaatg cggagtatgg
      961 tgccaacctt aagcaagttc atgtttttct cgaagaaagc acttcgattg gctcgataca
     1021 acgcaaccaa catttgattt agatcgactc catctggcgt tacttgccgg gcaattcttg
     1081 ttaaagtatc gcctttaacg acgggaccgt aagtatcgcc agctgtacta gatgttctag
     1141 cttttggggt gggttgtttt ggtttctgct tgctttgatt attctgtgct gatgttgtgg
     1201 gtgtcccagc aatttgcggc gcagcttccg taaccggtga aacaacggga ctggcgggtt
     1261 ctgcgatatt ggtatctaca ggatcaagca ataccgtata ttcacgtagt aatcgacccg
     1321 aagaccaatt taattcgata agaagattta aaaacggctc attaatcgat tgcggtgaac
     1381 taatctgaat atagggttga ccattcgcac gcttttcaac tgaaataact aacgatgaat
     1441 gataaggtgc aagtgcaaca ccggcctgac gaaaggcttc gggtgaagca actcgagcca
     1501 ccagcgatgg gatttcatcg ttcttgaccg ataccaactc gatctctgct cttagcggtt
     1561 gcccaagtga tgagcctaag gttaacttgc ctaatccagc cgcattaact agaaaaggaa
     1621 atgcaatcaa tactactaac caacacgccc taagatagat tttattcact gggttttcct
     1681 cacaattttt agaacagatt gtatgccaat atcaagccaa ttggcaaaac aagtaataat
     1741 aatcaagtat tagaatgata aatgattctt gactattcta tttgacttga ataaagaggc
     1801 aaaatggctt cgattctaaa agtaattaat tttctaccag tattcttaac attcttcgta
     1861 atggctcagc agccccccag agcaattgat cgcccacagt gaaagctgtt aaatactcac
     1921 cccccatgct caatttacgg atacggccaa catgaacatc taattttcct gtaaccgcaa
     1981 ctggggtaag ctcctttgtt gttttctctc tttcatttgg aatcacgcgg acccaatcat
     2041 ttgattgggc gataacatcc tcaatttcat ttaaaggaat atcttgcttt aattttatag
     2101 ttaatgcctg actatggcat cgcatagcgc caattcggac gcaaagacca tcaacaggaa
     2161 ccggcttgct ttgatgccca agaattttgt tcgtttccgc ttgacccttc cattcttctc
     2221 tactttgtcc ttgcgctact tcacgatcaa tccagggaat caggctaccc gctaatggaa
     2281 caccaaaatt ttcaactgga aattttttat ccctaagttt ttccgaaact tctcgatcaa
     2341 tgtccaatat gcttgaagcg gggttattta ataattcttc tgcaacatga tgcgcctctc
     2401 ccatttgctg taaaagctcg cgcatattct gagcacccgc acccgatgcc gcttggtaag
     2461 tcatggcgct catccattcc accatattat tttcaaatag cccgccaata cccatcaaca
     2521 ttaaactcac cgtgcaatta ccaccgatat agttttttgt gccttcatgc aaggcctgtt
     2581 taatgactgg catattaaca ggatcaagga tgattactgc atcatccttc attcttaacg
     2641 tcgacgctgc atcaatccag tagcctttcc aaccagcctc acgtaatttt ccaaagactt
     2701 cattagtata atcaccacct tggcatgtga tgatgatatc catcttcttg agttcagaaa
     2761 gatcatgggc gtctttgagc ggaggaattt cttggcctat atccggacct ttgccattct
     2821 tctgagaggt agagaaaaaa ataggctcta ccagtgaaaa atcattttct tcgcgcatac
     2881 gctgcatgag aacggatcca accattccac gccaacctac aaaaccaacc tgtttcattt
     2941 aattaaatta acctttgtta aaatagttct tgtattaaat atgcacagcc tgtttagatt
     3001 acaagttgtg attactaata aagttacgat taagcaagaa aatgattgta aattcaaata
     3061 attttcatgg ctatagattt gcgagtactg catttcccat ttctgctgta ccaaccttaa
     3121 tcattccttg ttcaaagata tcgacagtac gataacctga tgctagtact tttttaactg
     3181 catcttcgat gcgtgacgct gccatatctt gattaaaggt ataacgcaac atcatcgaaa
     3241 cagatagcag cgtagctagg gggttagcta aatttttacc ggcgatatct ggagcagaac
     3301 catgaatcgg ttcatataat cctttattaa actcgtttaa cgaagcggaa ggtaacatgc
     3361 caattgagcc ggttagcata gatgcttcgt cggatagtat atccccaaac atattgcctg
     3421 ttacaatgac atcaaattgc tttggattgc gaattaactg catcgcagcg ttatcgacta
     3481 gcatatgaga aagagccaca tccgggtatt cttttgcaac ttcttctacg acattacgcc
     3541 ataattgggt acattccaac acattcatct tatcaactga acacaacttg ctacttcttt
     3601 tgcgtgcggc ttgaaatgct acatgggcga tgcggcgtat ttcagattct gtatagatca
     3661 tcgtattaaa accaacccgt tgcccatcgc gtacttcaat accacggggt gttccaaagt
     3721 aaatatcgcc agttagttcg cgaacgataa gaatatctag attggctaca atttctggct
     3781 tcaatgtgga ggcattggcc aattctggat aaagttgcgc tgggcgtaga tttgcaaaga
     3841 aatttaatgc tttgcgaatt gctaatagcc cttgttcagg gcgtttttcg cgtggtaatt
     3901 gatcgtactt aggcccaccg actgccccca gtaagatagc atcagcttct tcagcgaggc
     3961 ggtgcgttgc ttcaggaaaa gggtcgttgt atttatcgac cgctgaaccg ccaatgaagc
     4021 catgttctaa ttcaattttt aaaccatcac tcgcaagcgc cattaggatt ttttcagctt
     4081 gctgcgtaat ttctggaccg atcccatctc cgggtaaaac tgctattttc attgcgtgat
     4141 ttgaattgtg tgaatgcgat taaataaaca accaaggttg ttcactttgt cgtttttgct
     4201 cgaaaagctt aattttatct gtgtgctgta atgtcaagcc gatttcatct aatccattga
     4261 ttaaacaatg tttcctaaat gagtccacat cgaaatgata acattctttc gtgggtgtcg
     4321 tgacagtctg tgcttctagg ttaattgcta agcgataacc ttctgtcgct tgg
//
LOCUS       DAOQLJ010000173         4293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_9993_length_4293_cov_5.820198, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000173 DAOQLJ010000000
VERSION     DAOQLJ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9993_length_4293_cov_5.820198"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /gene="groL"
                     /locus_tag="PK522_10165"
     CDS             <1..1503
                     /gene="groL"
                     /locus_tag="PK522_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110680.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNC42137.1"
                     /translation="TITKDGVSVAKEIELKDKFENMGAQMLKEVASKTSDVAGDGTTT
                     ATVLAQSIVKEGMRYVAAGMNPMDLKRGIDKAVIATIEDLKKLSKPCSTSKEIAQVGS
                     ISANSDAEIGKIIAEAMEKVGKEGVITVEDGSGLQNELEVVEGMQFDRGYLSPYFVSS
                     AEKQIALLESPFVLLHDKKISNIRDLLPVLEQVAKAGKPLLIIAEDVDGEALATLVVN
                     NIRGILKTCAVKAPGFGDRRKAMLEDIAILTGGTVVAEEVGLSLEKVKLDDLGQAKRI
                     EVGKENTTIIDGAGDVKSIEGRVAQIRTQIEEATSDYDKEKLQERVAKLAGGVALIKV
                     GAATEVEMKEKKARVEDALHATRAAVEEGIIPGGGVALMRAIGTVGKLKGDNHDQDAG
                     IKIVERALEEPLRQIVANCGDEPSVVANKVKEGKGNFGYNAATGEYGDMVAMGVLDPT
                     KVTRSALQNAASVAGLILTTDAMVAELPKDESASPAPGMGGMGGMGGMDM"
     gene            1599..2351
                     /locus_tag="PK522_10170"
     CDS             1599..2351
                     /locus_tag="PK522_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC42138.1"
                     /translation="MENFSNYQADKDLLRDRVILVTGAGQGIGRQAAITYAACGAIVI
                     LHGRKTKKLENVYDEISELKREEAVIFPFDFEKATENDFYTIAESIASQLGRLDGILH
                     NAAFTYGPMPFEHHTLEHLQTLMHVNLLAPITLTKACLPLLKLSNDASIVMTSDDHGH
                     VPSAFWGAFAIAKAGVEAYVKIQSEEWTLLPNIRINGLVPGPVHSPQRVKTHPGELKS
                     KLLQPSDLMGTYLYLMGPDSKGRSGEVLFCQS"
     gene            2423..2507
                     /locus_tag="PK522_10175"
     tRNA            2423..2507
                     /locus_tag="PK522_10175"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2457..2459,aa:Leu,seq:tag)
     gene            2529..3839
                     /gene="tig"
                     /locus_tag="PK522_10180"
     CDS             2529..3839
                     /gene="tig"
                     /locus_tag="PK522_10180"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNC42139.1"
                     /translation="MQSNTESPNALERKISISIPQEDVRVEVDNRLKKLSGKVKLPGF
                     RPGKVPLKMVTQQYGLQLHQEVLSELVQKHFRDSLQKQDMRIAGTPNFSSKSLDGDSL
                     NYEFDAVFEVYPEITIGNFSDLNVSKPGVQIGEAEVEKTLDTLRKQRAKFESIDRPAG
                     KGDRVNIDYHGQVDGKEFEGGQAKGFSLILGDGYALKDFEDAIIDMKNGENKVFQMIF
                     PTDYHGKEVAGKSVTFDVRLNKVEEQLLPSIDSDFAKSLGISDGDINKMYAEVKVSIE
                     REVLQRIRTKLKEQVMQFLLESTNCGIPKVLVNHEIEHLLQEANNNLKARGLADREAN
                     LSPDLFRERAERRVKLGLILAKLIEQQGIKVQSDQLRKYVEEYAKSYENPEQVINWYY
                     SSPERLKDIESVVLEDNVVTWILERAHITEQQMSFDDLMGYSKH"
     gene            3885..>4293
                     /locus_tag="PK522_10185"
     CDS             3885..>4293
                     /locus_tag="PK522_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNC42140.1"
                     /translation="MVIETSGRGERAYDIYSRLLKERIIFLVGPVTEVTANLVVAQLL
                     FLESENSDKDIYLYINSPGGSVSAGLAIYDTMQFIKPNVSTLCIGQAASMGALLLTAG
                     EKGKRFCLPNSRVMIHQPMGGFQGQASDIEIHAK"
BASE COUNT         1391 a          688 c         1032 g         1182 t
ORIGIN      
        1 actattacta aagatggtgt atcggtagca aaggaaattg aacttaaaga taaattcgag
       61 aatatgggtg cccagatgct taaagaggtt gcaagtaaaa cctctgatgt agcaggagat
      121 ggtacaacga cagcaacggt attagcacaa tcaattgtga aagaaggaat gcgttatgtc
      181 gcggcgggta tgaatccaat ggatttaaaa cgcggaatcg ataaagcagt tatagcaacc
      241 attgaagacc taaaaaaact ttctaaaccc tgttctacca gcaaagaaat tgctcaagtt
      301 ggcagtattt ccgcaaattc agatgcggag attggaaaaa tcattgctga ggcaatggaa
      361 aaagttggca aagaaggtgt aattacggtt gaagatggct caggtttaca aaacgagctt
      421 gaagttgtag aaggtatgca atttgatcgg ggttatttat ctccttattt cgtcagtagt
      481 gcggaaaaac aaattgcatt gctagaaagt ccctttgtat tgttacatga taagaaaatt
      541 tctaacattc gcgatttgtt acccgttcta gagcaagttg caaaagcagg gaagccttta
      601 ttgattatcg ctgaagacgt tgatggagaa gcgcttgcaa cattagtggt caataatatt
      661 cgcggtattc tcaaaacttg tgcagttaag gcacccggtt ttggtgatcg ccgtaaagcg
      721 atgctggaag atattgcgat attgaccggt ggaacggtag ttgccgaaga agttggatta
      781 tcgcttgaaa aagttaaatt ggatgactta ggtcaagcca agcgaatcga agttggaaaa
      841 gaaaatacca ctattattga tggtgctggt gatgtgaaat cgatcgaagg gcgtgtagcg
      901 caaattcgta cacaaattga agaagcaacc agcgattacg ataaagaaaa attgcaagaa
      961 cgtgtagcaa aacttgctgg tggcgttgca ttgattaaag taggtgctgc gacagaagta
     1021 gaaatgaaag agaaaaaagc ccgagtggaa gacgcactcc atgcaacccg tgcagcagtg
     1081 gaagaaggta tcatccctgg cggtggcgtt gcgctaatgc gtgctattgg tactgtaggc
     1141 aaacttaaag gtgacaatca tgaccaagat gctgggatta agatagtaga acgagcatta
     1201 gaagaacctt tgcgtcagat tgttgcaaac tgtggtgatg aaccatcagt tgttgcaaac
     1261 aaagtaaaag aaggtaaagg aaattttgga tataatgcag cgacaggtga gtatggtgac
     1321 atggtcgcaa tgggtgtatt agatcctact aaagttaccc gttcagcgct tcagaacgca
     1381 gcttcagttg caggacttat cctaacaacc gatgcaatgg tagcggaatt gccaaaagat
     1441 gaatctgctt cacctgctcc gggtatgggg ggcatgggcg gtatgggcgg catggatatg
     1501 taacgctgtt actcagaaag cgacgagtaa tctatcccta atcgctacaa caataggtct
     1561 tcaaggcttg ttgttgtggc gatatttttt attgatatat ggaaaatttt agtaattatc
     1621 aagctgataa agatttatta agagaccggg tcattttagt aacaggtgcg ggacagggaa
     1681 ttgggcgcca ggccgcaata acttatgctg cttgtggagc aatcgttatt cttcatggtc
     1741 ggaaaactaa gaagcttgaa aatgtgtatg acgaaatcag tgagctaaag cgggaagaag
     1801 cagttatttt cccctttgat tttgagaaag caactgagaa tgatttttat acaattgcag
     1861 aatcgattgc gtcacaatta ggaagacttg atggaatttt gcataacgct gcttttactt
     1921 acggcccaat gccttttgag catcatacac ttgagcactt gcaaacatta atgcatgtta
     1981 atttgcttgc cccgataaca ctaacaaaag catgtttgcc cctactcaaa ttatctaatg
     2041 atgcgagtat tgttatgaca tctgatgacc acggtcatgt gccttcagcc ttctgggggg
     2101 catttgctat cgcaaaggca ggggtggaag cttatgtgaa gattcaatct gaagagtgga
     2161 ccttattgcc gaatattcgt attaatgggc ttgttccagg gccagttcat tcaccacagc
     2221 gtgttaaaac gcatcctgga gaactgaaaa gtaaattgtt gcaacctagt gatttaatgg
     2281 ggacttatct ttatttaatg gggcctgata gcaaaggtag aagtggcgag gtactatttt
     2341 gtcaatccta gagtcctttg gctgctatcc caaccataac aaggtataat tcacaaacga
     2401 aaaaaattaa ttagttgaaa aagcgaaagt ggcggaattg gtagacgcac cagatttagg
     2461 ttctggcgcc gtaaggtgtg ggggttcgag tccccccttt cgcaccaaac ttgtttagga
     2521 agagtccaat gcaatcaaat acagaaagcc caaacgcgct cgaacgaaaa atcagcattt
     2581 ctatacccca ggaagatgtt cgcgttgagg tggataatcg tttaaaaaag ctatcaggaa
     2641 aagttaaatt gccaggtttc cgtcctggca aggtgccatt gaaaatggtg acgcagcagt
     2701 atggtctaca attgcaccag gaagttttga gtgagttggt acaaaaacat tttcgtgatt
     2761 cattgcaaaa gcaagatatg cgaattgcag gtacgccaaa tttcagttct aaatcgcttg
     2821 atggagatag cttaaattat gaatttgatg ctgtttttga agtctatcca gaaatcacga
     2881 ttggaaattt tagcgatctt aatgtttcta aacctggtgt acaaataggg gaagctgaag
     2941 ttgagaaaac attggatact ttgagaaagc aaagagcaaa atttgaatca atcgatagac
     3001 ctgcaggaaa gggtgatcgg gtaaatattg attatcatgg acaggtcgat ggaaaggaat
     3061 ttgaaggagg acaggcaaaa ggttttagcc taatattagg cgatggttat gcattaaaag
     3121 actttgaaga tgccattatt gatatgaaaa atggcgaaaa taaagtattt caaatgatat
     3181 ttcctacaga ttaccatggt aaagaagttg caggtaaatc cgtaaccttt gatgttagat
     3241 tgaacaaagt agaagaacaa ttacttccaa gtattgacag tgattttgct aagtcacttg
     3301 gtatcagcga tggagacatt aataaaatgt acgctgaggt aaaagtgagt attgagcgtg
     3361 aggtattaca aagaattcgt actaagctaa aagagcaagt gatgcaattt ttacttgaaa
     3421 gcacaaactg cgggatacct aaagtgttag taaatcatga gattgagcac ttacttcaag
     3481 aagctaataa caatctaaag gcacgaggtc ttgctgatcg agaggccaat ttatcgcctg
     3541 atttattccg agaaagagct gaacgacgtg ttaagttagg tttgatttta gctaaactca
     3601 tagaacaaca aggtataaaa gtacaatcag accagttaag aaagtatgta gaggaatatg
     3661 ctaaaagcta tgaaaatcct gaacaagtca ttaattggta ttattcatcg cctgaacgat
     3721 taaaagatat cgaatcggta gtattagagg ataatgttgt aacgtggata ttagaaagag
     3781 cacatattac tgagcagcaa atgtcatttg acgatttaat ggggtattcg aagcattaat
     3841 ttacgggggt gaatttggat atcaatggat taggtttaat tccaatggtc attgagacca
     3901 gcggaagagg tgaaagagca tacgatatct attctaggtt attaaaagaa aggataattt
     3961 ttcttgttgg gccagtgact gaagtaacag ctaatttagt tgtagctcaa ttattatttc
     4021 tagaatctga gaactctgat aaagatattt atttatatat taattcacct gggggttctg
     4081 tctcagctgg tctcgcaatt tatgacacga tgcaatttat taaacctaat gtgagtacac
     4141 tatgtattgg tcaggcggca agtatgggcg cactattatt aactgctggt gagaagggta
     4201 aacgtttttg cttgcctaat tcacgggtaa tgattcacca gccgatgggt ggttttcaag
     4261 gccaagcatc tgatatcgag attcacgcta aag
//
LOCUS       DAOQLJ010000174         4273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_10068_length_4273_cov_5.379090, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000174 DAOQLJ010000000
VERSION     DAOQLJ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10068_length_4273_cov_5.379090"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..2373)
                     /locus_tag="PK522_10190"
     CDS             complement(145..2373)
                     /locus_tag="PK522_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC42141.1"
                     /translation="MEARSKMKHSENTPDLVTKKSSNESLYIGQFIEWQNLIPWLQWL
                     DELVDKASKRIPQVYGIEATQDAFRGLHISQEEFQRLVAREPGSSLFAVADQIDSDYH
                     LNKLPLPEQLYQLLQSFSLSRFDICILIIALAPELDLKYERLYAYLQDDVSKKRPSVD
                     LALNLLCPSALEKMQRRRHFATDASLIANELVHLIADTNQSQPPLLAHYLKLDDQIVS
                     FILNEQSIDKRIVSACNLTFPAGNLRSNAIINDAEKDLPALAKKRIEAGKPLWLYFQG
                     LDTTSKTALAENLASQLSMPILQVDLARLLNAAADFKTACRLTLREARLKGAILYLNE
                     YDTLRESEHAAAHQQFLTQLAQMNGIMILSGIGPWIPHEQGPKGILSVTFDLPDFSQR
                     LKIWKNTLKTQGIKLSKKDVESLARRFRFTPSQITDVVITATNRAQLQTFNSSESNQS
                     RTLLYGLFAAARIQTRHILAKLAQHIEPKSQLDDLVLPEDAMTQLKEIIVQYLHRETV
                     LETWGFGRKLSYGKGINVLFAGSSGTGKTMAAEILASQLGLDLFRIDLSGVVSKYIGE
                     TEKNLDKIYTAAENANAILFFDEADALFGKRSEVKDSHDRYANLEISYLLQKMEQYEG
                     IAILATNLRQNLDEAFVRRLAFIVHFPFPDEAMRRRIWAGIWPASVPLAEDVDLDTLA
                     KQFKLSGGNIRNIALSAAYLAAGNGQGVTRAQIQQAAQREFQKLGKLLAQTGEGLSRV
                     PI"
     gene            complement(3041..3706)
                     /locus_tag="PK522_10195"
     CDS             complement(3041..3706)
                     /locus_tag="PK522_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42142.1"
                     /translation="MGKKQRTSKPTEDSKRDFPRKAIPLEIDKNEAETCRNHAQIITS
                     PEVATYRVIQACESQALIDEIDVPSLIEVLRNQSMAANNNDLSQAEGMLTNQAVSLQG
                     LFVRMIEKAFQQNHPPHFEVLMKLALKAQSQSRATIETLSAIKNPPTVFAQQANIAHG
                     HQQVNNDNHTASHARENQKQQNELLRDSNEAVDSRRATTAIATNQELATLGAINRCNN
                     LTR"
     gene            3974..4153
                     /locus_tag="PK522_10200"
     CDS             3974..4153
                     /locus_tag="PK522_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42143.1"
                     /translation="MARKASGTKQLEIAMERLKKVKTAAELRAAEAIVLPLLLGLSLE
                     QTARAIGRSVGITCG"
BASE COUNT         1106 a          890 c          989 g         1288 t
ORIGIN      
        1 cctgattacc agctttcccc tgcaggctca gtagttgctg cgcagggctc tgtaagtcag
       61 accgaccgaa tgagttcaag cccggctcga cttgccggtg caccggcgtg cttttgttgt
      121 ctgcgctctt ggcggcttcc ttgctcatat tggtactctg gataggcctt cacctgtctg
      181 cgcaagcaac ttacccagtt tctgaaactc ccgctgtgca gcctgctgaa tctgtgcacg
      241 ggtgacgccc tgtccgttgc ctgcagcgag atatgcagct gacagcgcga tatttctgat
      301 attcccaccg ctgagcttga actgtttcgc cagtgtatcg agatccacgt cctcagcgag
      361 ggggaccgaa gccggccaga tgccggccca gatgcgacga cgcattgctt catctggaaa
      421 tgggaaatgc acgatgaacg ccaaccgccg tacaaacgcc tcgtccagat tctggcgcag
      481 gtttgtggcg aggatggcga tgccttcgta ctgttccatc ttctgaagca aatagctgat
      541 ctccaggttg gcatagcggt cgtgggaatc cttgacttcg gagcgtttgc cgaatagggc
      601 atcggcttcg tcaaagaaca ggatcgcatt ggcgttttcg gcggcagtat aaattttatc
      661 cagatttttt tcagtttcgc caatgtattt actcactacg ccggataaat cgatcctgaa
      721 taaatccaat cctaattgag atgccagtat ttcggcagcc atggttttgc cggtaccgga
      781 tgagcccgcg aacagcacat taattccttt gccgtaggaa agtttacggc cgaaccccca
      841 ggtttcaagc acggtttcac gatggaggta ttgcacgatt atttctttaa gctgggtcat
      901 cgcatcttcc ggcaatacca gatcatccaa ttgcgattta ggttcgatat gctgagccaa
      961 cttggcgaga atgtgccggg tttgtatgcg agctgccgcg aataagccat agagcaaagt
     1021 gcgtgactga tttgattcac ttgaattgaa cgtttgtaat tgcgctcgat tggttgctgt
     1081 gatgacgaca tcagtaatct gactgggtgt gaagcggaaa cgtcttgcaa gcgattccac
     1141 atctttttta gagagcttga ttccttgggt ttttaaagtg tttttccaga ttttaagccg
     1201 ttgcgagaaa tctggtagat cgaatgttac cgataggatt cctttgggtc cttgttcatg
     1261 aggaatccag gggcctatcc cagaaagaat catgatgcca ttcatttgag ccaattgcgt
     1321 taaaaattgc tggtgtgcag cagcatgctc cgattcccgg agtgtgtcgt attcattcag
     1381 atataaaata gcccctttta accgagcttc acgcagggta agccggcagg ctgttttaaa
     1441 atccgctgca gcattcagta atcttgctaa atctacttgt agtatgggca tacttagctg
     1501 gctggctaag ttttcggcta atgctgtttt gctggttgtg tctaaccctt ggaagtaaag
     1561 ccataggggt ttgcctgcct caatgcgttt cttggctaaa gcaggcaaat ctttttcagc
     1621 gtcattgatt atggcgttgc ttcttaaatt accggccgga aatgtaagat tgcaagcaga
     1681 tacaattctc ttgtcgatac tttgttcgtt caatataaag gagacaatct gatcgtcgag
     1741 tttcagataa tgcgcgagga ggggcggctg tgattggttg gtatctgcaa tcagatgtac
     1801 aagttcattt gcaatgagtg acgcatcggt tgcaaagtgc ctgcgccgct gcattttctc
     1861 cagtgcactg gggcagagca gattcaacgc taaatcgaca ctggggcgtt ttttactcac
     1921 gtcgtcttgc aggtaagcat acaaccgttc gtatttcaaa tctaattccg gtgcaagcgc
     1981 tatgatcaag atgcaaatat caaaccggga taacgaaaaa gactggagta gttgataaag
     2041 ttgttccggc agaggaagct tattaaggtg ataatccgaa tcgatttggt cggctacagc
     2101 gaataatgaa cttcccggtt cacgtgctac taggcgttga aattcttctt gagaaatgtg
     2161 taaaccacgg aaagcgtctt gagtggcttc aatgccataa acctgaggta ttctttttga
     2221 tgctttgtcg accagctcat ctaaccactg caaccaagga atcaggtttt gccactcaat
     2281 aaactgccca atatataaag attcgttact acttttttta gtaactaagt caggcgtatt
     2341 ttctgagtgt ttcattttag accgtgcttc catgaagtaa ctcaacaaat atttttcatt
     2401 acattaatgt aggcagtaat aaattgtatg tcctttcaaa attaagctct tgttcagtct
     2461 gaaagtcaac aaaattatga aatgcacgtt ggcatcactg attgagcaca agctatggga
     2521 tgcaataatc ctactgacgc caagcaagta aattattcca ttaacacaaa cggttgttgc
     2581 tgcgatgaaa ccaccggtgg caagaatata aacgatcata atggataaca gtttggcatt
     2641 tcctgggtag gtttttcttg caaaggtatt gaatgcgaca atcgattttg cacattttta
     2701 tgcagcagga agacgtaggg cgaatgaaaa cacaaataga cttataagac agcatctctc
     2761 aataggaact gacttcaatc gggcgactgt tagataaatt aaacatgtta tggatcagtt
     2821 aaacaaccca aaacccataa aaccaagaga aataaatcac ataatgaatt attttgggga
     2881 caaaaattga tttacttgct gcataattta ttgtatttat gattcgaatc tacccatctc
     2941 aaatcgctca agaaactgct cttgctgttt caatactgcc cgtatttctt ttttaagttt
     3001 gccaattcca tgcttatagg cattttgact cgaacgcgct ttaccttgtg aggttgttgc
     3061 accggttgat tgctcccaag gtcgccagtt cttgattagt tgcgattgcc gtagtcgctc
     3121 ttctgctgtc cactgcttca ttagagtccc ttaatagttc gttttgctgt ttttgatttt
     3181 cccgcgcgtg cgaagctgta tggttatcgt tattaacttg ctgatgccca tgagcaatat
     3241 tagcttgctg tgcaaaaaca gtgggtgggt ttttaatcgc agataaagtt tcgatggttg
     3301 cacgagactg cgattgtgct ttgagtgcga gtttcatcaa tacttcgaaa tgcggtggat
     3361 ggttttgttg aaatgctttc tcaatcattc gaacgaatag gccttgtaag gatacagcct
     3421 gatttgtgag cattccttct gcttgtgata ggtcattgtt attcgccgcc atggattggt
     3481 tacgcagtac ttcgattaga cttggtacat caatttcgtc tataagcgct tgagactcac
     3541 aagcttgtat tacacgatag gttgccactt caggtgaagt gattatttgc gcgtgattgc
     3601 gacaagtctc tgcttcgttt ttgtcaatct ccaaaggaat agcttttcgg ggaaaatcgc
     3661 gcttggaatc ttctgttggt ttgctcgttc tttgtttctt acccataaat ttgctcctcc
     3721 aattttaaag caaggatttt ttcactgatc aaaacataga gtaggttgag tattttatct
     3781 tcatggtaaa aattaggctc tcaactgtaa tatgcatatt gcaaatcaca ttattatcgt
     3841 tgcttcgtta tattaatacg ataaatcaat tagttagtcg tgatagaaac agcacccaat
     3901 tgcttgagat aagtaattcc aattacagat agaaacagta ataatatgac ctttaccttt
     3961 tgtagaggcc ctgatggcac ggaaggcaag tgggacgaag caacttgaga tagcgatgga
     4021 acggttaaag aaggtcaaga ctgcggcaga actccgggct gcggaagcga tagtgctacc
     4081 attgttgcta ggtttgagtc tggaacagac tgcgcgtgct attggacgtt cggtaggcat
     4141 cacctgtgga taagtataac attaagaaat tgatgtcatc aatatccata tgctatcatt
     4201 gaactcaaat aatgaggggt gaagtcaaat gtctgttgtt actgttcgca atttgcctga
     4261 agaaacgcat cgc
//
LOCUS       DAOQLJ010000175         4270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_10085_length_4270_cov_6.481139, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000175 DAOQLJ010000000
VERSION     DAOQLJ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10085_length_4270_cov_6.481139"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..724
                     /gene="cas8c"
                     /locus_tag="PK522_10205"
     CDS             14..724
                     /gene="cas8c"
                     /locus_tag="PK522_10205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HNC42144.1"
                     /translation="MAVRFWHVKTVGELAVNIRRHFDDLRIVHAPHEPETLSLFRLLV
                     ATAMLGKSENIPPNFGGDVMRSVIEGSPYPQTLLSGTIRRIRAEHEVTYPRASIIKAC
                     LNRQSGKEELKVSLDENNTNTAYRLGRLFAVLERIQERTNPNINATIRDRYYGAASGT
                     PVTVLPTLLKLKNHHLAKLDNKGEVINYERALGQIMDGITDFPAQLNLQDQGRFAIGY
                     YHQRQAFFTKSESTIKGE"
     gene            729..1610
                     /gene="cas7c"
                     /locus_tag="PK522_10210"
     CDS             729..1610
                     /gene="cas7c"
                     /locus_tag="PK522_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HNC42145.1"
                     /translation="MALNNRYDFVLLFDVRDGNPNGDPDAGNLPRVDAETGRGLVTDV
                     SLKRKVRNFVGLTKEEQPPFEIYVKEKAILNNQNKRAYMGIGKAELLEGDDKKRKGGD
                     AVDEARQWMCKNFFDVRTFGAVMSTGINCGQVRGPVQLTFARSVDPIVAQEHSITRMA
                     VATEAEAEKQGGDNRTMGRKFTVPYGLYVAHGFVSAHLANQTGFNEDDLELLWQSLVN
                     MFEHDRSAARGEMATRGLYVFKHDSQLGNAPAYNLFERIQPKLKDGIVVPRSFEDYQV
                     NVNETSLPSGVTLERKA"
     gene            1613..1828
                     /locus_tag="PK522_10215"
     CDS             1613..1828
                     /locus_tag="PK522_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42146.1"
                     /translation="MKNANEIIFLVEEAPEGGYTARALGESIFTEADDLANLHQQVRD
                     AVHCHFDEGKAPEIIRLHFVREEVIQT"
     gene            1825..2085
                     /locus_tag="PK522_10220"
     CDS             1825..2085
                     /locus_tag="PK522_10220"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNC42147.1"
                     /translation="MKLPRNISGARLIKALNRLGYGITRQVGSHVRLECQLPKPHHLT
                     VPIHDPLRVGTLASILNEIARHHSLSKEEVILRLFGKINDHN"
     gene            2072..2698
                     /gene="cas4"
                     /locus_tag="PK522_10225"
     CDS             2072..2698
                     /gene="cas4"
                     /locus_tag="PK522_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HNC42148.1"
                     /translation="MTTIEVDEPIMLSALQHYSYCPRQCALIHVEQAFDENVHTMRGN
                     TVHQRVDEPGSESFEGVRSEKALPIWSERLGLIGKCDIVEFYSNGRIYPVEYKHGKKR
                     AKAHDDIQLAAQAICLEEMTGKTVTHGAIYHHSSRRRREVVINELLRQQVEETVQAVR
                     ALLVSGKLPPPVNDVRCKECSLKEICQPEVLADKARVLDAQIHLFEVG"
     gene            2708..3742
                     /gene="cas1c"
                     /locus_tag="PK522_10230"
     CDS             2708..3742
                     /gene="cas1c"
                     /locus_tag="PK522_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HNC42149.1"
                     /translation="MYQILNTLYIMTPNAYVHLDNDTVRIDVERQKKLQVPLHHLGTL
                     VCFGNIMISPALMHRCADDGIGLVLLDANGRFKARLEGAVNGNILLRQMQHRKAIEFP
                     FSLAIAKAVIAGKLRNCRQVLLRGAREATDEQDQGVLSNAAATLANSIRNLPTAADLD
                     VIRGIEGDAAKVYFSALPNLVRHNIREYFTMDGRTRRPPRDRFNALLSFLYSLVMNDC
                     RSAIESVGLDPQLGFLHSVRPGRAALALDLMEEFRSILADRLALTLINRSQITEHDLK
                     IREGGAVQLENDARKVVVAAYQEKKQEEVTHPLLNTKVAFGLLPQLQARFMARAIRGD
                     IDSYIPFLIK"
     gene            3745..4035
                     /gene="cas2"
                     /locus_tag="PK522_10235"
     CDS             3745..4035
                     /gene="cas2"
                     /locus_tag="PK522_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNC42150.1"
                     /translation="MLIIVTYDVSTETKEGRRRLRRVAKICEGIGQRVQKSVFECKVD
                     LMQYEELERRLLAEIEEKEDNLRLYRLTEPVALHVKEYGKFKAIDFDGTLVV"
BASE COUNT         1157 a          920 c         1067 g         1126 t
ORIGIN      
        1 taatgctgcc cgtatcgctg tgcgcttctg gcatgtcaaa accgtgggcg agctggctgt
       61 caatattcgc cgtcacttcg atgatctgcg catcgttcat gcaccgcacg aaccggaaac
      121 actgtcgctg ttccgcctgc tggtcgcaac agcgatgcta ggcaaatcag aaaacattcc
      181 gcccaatttc ggcggcgatg ttatgcgcag cgtgattgaa gggtcgccct atccgcagac
      241 gctgctttcc gggacaattc gccggattcg agccgaacat gaagtcactt acccgcgcgc
      301 ttcgatcatc aaagcgtgcc ttaatcgtca atcaggcaag gaggaactca aagtgtcact
      361 ggacgaaaac aacacgaata ccgcttaccg gcttggccgg ttgtttgccg tactggagcg
      421 tattcaggaa cggacaaatc ccaacattaa tgcaacgatc cgtgatcgct attacggagc
      481 ggcttcaggt actccagtca ctgttttgcc aacgctgctt aagcttaaga atcaccatct
      541 agctaaactg gataataaag gtgaagtcat caattatgaa agagcgctag gacagatcat
      601 ggatggtatt accgattttc cagcacagct caatttgcaa gaccaaggcc gttttgccat
      661 tggctattac catcagcggc aagctttttt taccaaatcc gaatctacaa tcaagggaga
      721 ataatcgcat ggcacttaac aaccgttacg atttcgtttt gttgtttgac gtaagagatg
      781 gtaatcccaa tggcgatccg gacgcaggca atttgccgcg tgtagatgcg gaaaccgggc
      841 gtggattagt aaccgacgtg agcctaaagc gtaaagtacg caattttgtt ggtttgacaa
      901 aggaagagca gccgccattc gaaatctatg ttaaagagaa agcaatcctc aataatcaga
      961 ataaacgcgc ctatatgggt atcggcaaag ctgaacttct tgaaggtgat gataagaagc
     1021 gtaagggtgg cgatgctgtg gatgaagcaa gacaatggat gtgcaaaaat ttctttgatg
     1081 tacgtacctt tggtgccgtc atgtcgacgg gtatcaactg cggacaagtc cgcggccccg
     1141 ttcaactaac ctttgcccgt tccgtcgatc cgattgtggc gcaagaacac tccatcaccc
     1201 gcatggcagt tgctaccgaa gcggaagcgg agaagcaagg cggcgacaat cgcacgatgg
     1261 ggcgtaaatt caccgtgcca tacggcctct atgtcgcaca cggtttcgtt tcggcgcatc
     1321 ttgccaatca gaccggattc aacgaagatg atctggaatt actctggcaa tcgttggtga
     1381 atatgttcga gcatgaccgt tctgctgcgc ggggtgaaat ggctacacgc ggtttatatg
     1441 tttttaaaca tgacagtcaa ttgggcaatg caccagccta caatctcttt gagcgtatcc
     1501 agcctaaact caaggatggt attgtcgtgc cgcgctcatt cgaggattat caagttaatg
     1561 ttaatgaaac cagtcttcca tccggtgtaa ccttggaaag aaaggcataa cgatgaagaa
     1621 cgctaatgaa attatttttc tggtcgaaga agcgcctgaa ggtggctata ccgcccgtgc
     1681 actgggagaa tccattttta ccgaagcgga tgatcttgcc aatctgcatc aacaggttcg
     1741 tgatgccgtg cattgtcatt tcgatgaagg aaaagcgcct gagataattc gcttgcactt
     1801 cgtgcgcgag gaagtcattc agacatgaaa ttgccgcgga atatatcagg agcacgctta
     1861 atcaaggcgc tgaaccggct cgggtacggg attacccggc aggttggcag tcacgtccgg
     1921 ctggaatgtc agctccccaa accccatcat ttaacggttc ctatacatga tccattgcgt
     1981 gtcgggacgc tggcttctat tttgaatgaa attgctaggc atcattcctt atccaaagaa
     2041 gaagtaattc ttagattatt tgggaagatt aatgaccaca attgaagttg acgaaccgat
     2101 catgctctct gcgttgcagc actatagcta ttgtccacgc cagtgcgcat tgattcatgt
     2161 cgagcaggcg tttgacgaga atgtgcacac catgcgcggt aataccgtgc atcaacgggt
     2221 tgatgaacct ggttccgagt catttgaagg tgtgcgcagt gagaaagctt taccgatttg
     2281 gtcagaacgt ttaggtctga tcggcaaatg cgatatcgtc gaattttatt cgaatggacg
     2341 aatttatccg gtggaataca aacatggcaa gaaacgcgca aaagcacatg atgatatcca
     2401 attggcagcg caagcgatct gcctggaaga aatgacgggt aaaacagtga cacatggtgc
     2461 gatttatcat cattcatcgc gccggcggcg tgaagtggtt attaacgaat tgcttcgaca
     2521 gcaggtggaa gaaaccgtgc aagcggttcg cgctttgctg gtatctggga aattgccacc
     2581 gccagtcaac gatgtacgtt gcaaggagtg ttcgttgaaa gaaatctgtc aacctgaggt
     2641 tttggcagat aaggccagag ttcttgatgc ccagatacat ttgtttgaag tggggtaata
     2701 gtaaattatg tatcaaattc tcaacacact ttacatcatg acacccaatg cttatgtaca
     2761 tttggataat gatactgtgc gcattgatgt tgaaaggcaa aaaaaactac aagtgccact
     2821 tcatcattta ggtaccttgg tatgttttgg taatatcatg atttcaccgg cgctgatgca
     2881 tcgttgtgca gatgacggaa ttggcttggt tttgttggat gctaatggtc ggtttaaagc
     2941 acgtctcgaa ggtgctgtaa atggtaatat tttgcttcgt caaatgcagc atcgcaaagc
     3001 gatagagttc cctttttcac ttgcgatagc caaggccgtg atcgctggga agttacgcaa
     3061 ttgccgccaa gtattgttgc gtggtgcgcg agaagcaact gatgagcagg accagggtgt
     3121 gcttagtaat gcagctgcaa cgttggcaaa ctccattcgc aatttaccta ccgcagcaga
     3181 tcttgatgtg atccgtggca tcgagggtga cgccgctaaa gtgtatttct cggcactacc
     3241 caatttagtt cggcacaata ttcgtgagta ttttacgatg gatggtagaa cacgtagacc
     3301 acctcgtgat cgcttcaatg cgctactatc atttctttat tcactggtga tgaacgattg
     3361 ccgttccgcg attgaaagtg ttggccttga tccgcaattg ggcttcttac atagcgtacg
     3421 ccctggcagg gcagcattgg cgcttgatct aatggaggaa tttcgctcta ttctcgccga
     3481 tcggcttgca ttaacactga ttaatcgtag tcaaattact gagcatgact tgaaaatacg
     3541 tgagggcggt gcagtgcagc tggaaaacga tgcacgaaaa gttgttgtgg ctgcttatca
     3601 ggagaaaaag caagaagaag tcacgcaccc attattgaat actaaagtag catttggctt
     3661 actgcctcag cttcaagcac gtttcatggc tcgcgctatt cgaggcgata ttgattccta
     3721 tattccattt ctgatcaaat aacgatgctg atcattgtca cttacgatgt ttctacagaa
     3781 acaaaagaag ggagaagacg gctgcgccgg gttgctaaaa tttgtgaagg aataggacaa
     3841 cgtgtgcaaa aatcagtgtt tgaatgtaaa gtcgatctca tgcaatatga agaattagag
     3901 cgcagattac tggccgagat tgaggaaaaa gaagacaatc ttcgtttgta tcgtttaaca
     3961 gaacctgttg ctttacatgt caaagagtat gggaaattta aagcaatcga ttttgatgga
     4021 accttggttg tttaggatgc gcgaacaacg agtgattgtg aaatagctat aggttcgcgg
     4081 aaggtataac attctgaatg ttaatagaat ttaataaagt aggcttttat ttgtgttgat
     4141 aaattttcta cttgctgccc agttcgcgca tttacaaaga ttttttctgt tgtatcatca
     4201 agttgtagct gtactgtagc gcccagcatc caagctgggc gaggattgaa acacggcatt
     4261 tacaaccgca
//
LOCUS       DAOQLJ010000176         4267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_10096_length_4267_cov_6.034425, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000176 DAOQLJ010000000
VERSION     DAOQLJ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10096_length_4267_cov_6.034425"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..534)
                     /locus_tag="PK522_10240"
     CDS             complement(34..534)
                     /locus_tag="PK522_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNC42151.1"
                     /translation="MARKASGTKQLEIAMERLKKAKTAAELRAAQAIVLPLVLGLSLE
                     QTARAIGRSVGVTCRMRTRYDQATGEEKKTTRSKRELRNRAKASLEQEARILEEVLSE
                     AATGGVVIVPPLKPAIEARLGKPLALSTLYQMLARHGWRKLAPDKSHPQADPAAREAW
                     KNSPSD"
     gene            694..981
                     /locus_tag="PK522_10245"
     CDS             694..981
                     /locus_tag="PK522_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HNC42152.1"
                     /translation="MLNKNILDEIGTKINEVIAHGPAKDIEKNLRTILTSMISKLDLV
                     TREEFDVQQEVLKRTRTKLTELETKVAALEGQLKPSPSETGTVTSDSNYDG"
     gene            1066..2580
                     /locus_tag="PK522_10250"
     CDS             1066..2580
                     /locus_tag="PK522_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNC42153.1"
                     /translation="MSLAILYSRALSGMDAPLVTVETHLSNGLPSFTIVGLPETEVKE
                     SKDRVRAALQNSHFKFPAQRITVNLAPADLPKESGRFDLPIALGILAATAQIPHDQLD
                     QFEWVGELSLSGELRPIKGALAMTYSASRSGRSFVLPKQNAVEAALVSDSIVYPAASL
                     LEICAHLCNETPLQRYSSTITPDTHSMNSPLTIDLQDVKGQVQAKRALEIAAAGNHSL
                     LMVGPPGTGKSMLAERLITILPTMSQQEALETAAIQSLAQGQFEIAHWKRRPFRSPHH
                     TASATAMVGGGSIPRPGEISLALNGVLFLDELPEFDRKVLEVLREPLESGHITISRAA
                     RQTNFPARFLLIAAMNPCPCGYLGHPSGKCHCTPDQVARYRGKISGPLLDRIDMQIEV
                     PSLPQNDLMQSQSIGEKSNIIRERVEKAHQLQYDRQGKTNSRLSVKEIDRLCVLDTAS
                     ENLLKQAINRLSLSARAYHRILKLARTIADLGNTEKINHQHIAEAIQYRRLDKH"
     gene            2745..3158
                     /locus_tag="PK522_10255"
     CDS             2745..3158
                     /locus_tag="PK522_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HNC42154.1"
                     /translation="MNDPDIRWQQRFANYQKALLQLQNAVELSNQRALSPLEKQGVIQ
                     AFEFTHELAWNLLKDFLQDQGNQNVRGSKDATREAFKVELIQDGEQWMAMIQSRNISS
                     HTYNEHTAEQLVNAIIHDYYPLFRALKKEIEKYQP"
     gene            3155..3478
                     /locus_tag="PK522_10260"
     CDS             3155..3478
                     /locus_tag="PK522_10260"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNC42155.1"
                     /translation="MNEQYSFGLSHTTLEKLNRVFARHDTIDSVLIYGSRAKGNYRRG
                     SDIDLTIKGSEISFAEFMQIEDQIDDLMLPYTVDLSQYRLLENTDLIAHIDRVGRVIY
                     TRPRK"
     gene            complement(3574..>4267)
                     /locus_tag="PK522_10265"
     CDS             complement(3574..>4267)
                     /locus_tag="PK522_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC42156.1"
                     /translation="CVLELGGSDPFIVLDDADIEWTAQQALMARLQNMGQSCIAAKRF
                     ILLETIADGFVERLQALISELRSGDPKDAATTIAPMARVDLRDELHAQVERSLALGAK
                     LRAGGFSIGEQGAYYAPTILDHVQPGMPAFDEELFGPVFAIARVSNAEEAIKLANQTQ
                     FGLGGSVWTGDLARGEALARQIHAGCTFVNGIVKSDPRLPFGGIKDSGYGRELSYHGI
                     REFVNIKTIWIR"
BASE COUNT         1178 a         1075 c          939 g         1075 t
ORIGIN      
        1 ggcgtggggt cccaaaagcc gccacttcct cggttagtcg ctcggggagt ttttccatgc
       61 ctcgcgcgct gctgggtccg cttgtggatg gcttttatca ggggccagtt tgcgccagcc
      121 atgccgcgcc agcatctggt acagagtgga caaagccagt ggctttccca gcctagcttc
      181 aatcgctggc ttcaggggtg gaacaatgac cacgccacca gtggcggcct cactcagcac
      241 ttcctctaga atcctggctt cctgctccag gctggctttg gcgcggttac gtaactcacg
      301 tttgctgcgc gtggttttct tttcttctcc cgtcgcttga tcataccggg tgcgcattct
      361 gcaggtgacg cctaccgaac gtccaatggc acgcgcagtc tgttccagac tcaaacctag
      421 caccaatggc agcactatcg cttgcgcagc tcggagttct gccgcagtct tggccttctt
      481 taaccgttcc atcgctatct caagttgctt cgtcccactt gccttccgtg ccatcagggc
      541 ctctacaaaa ggtaaaggct atattattac tgtttctatc tgtaattgga attagataat
      601 gatgagaaat gtttggattg gaaacctcgc ataaggtatg cttcgtatct ggacaaatac
      661 tgatgcattt gaaagacgct taatggagat atcatgctaa ataaaaacat tctggatgaa
      721 atcggcacga aaattaatga agttatcgcg catggtccag caaaggatat tgaaaaaaac
      781 ttacgcacga tcctcacgag tatgatctct aagcttgatc tcgtcacgcg tgaagaattt
      841 gatgtgcagc aagaggtact taaaagaacc cgtactaagt tgactgagtt agaaaccaaa
      901 gtagcagcgt tagaaggtca attaaaacct tcgccttcag aaactggaac ggtaacctcc
      961 gatagcaatt atgatggtta agcagccgat atttggaata aatctcctca ctaataagtc
     1021 attcccagca taaactaagt atttcagtat tgagcgcggt ataacatgtc tctggctatc
     1081 ttatatagtc gcgccctttc tggaatggat gcgccactcg tcacggtaga aacacaccta
     1141 tccaatggtt taccgagttt taccatcgtt ggtttacctg aaacagaagt caaagaaagc
     1201 aaagaccggg tccgggcagc cttacaaaat agccatttca aatttcctgc acaacgcatt
     1261 actgtcaatc tagctcccgc tgatctaccc aaagaaagtg gccgctttga tttacccatt
     1321 gcattaggaa ttctggcagc aaccgcacaa attcctcacg atcaactcga tcaatttgaa
     1381 tgggtcggtg aactttcgtt aagcggtgaa ttgcgcccga tcaagggcgc gctcgccatg
     1441 acttatagcg catcacgttc aggtcgcagt tttgtattac cgaaacaaaa tgctgtagaa
     1501 gcggcgttag ttagcgattc tatcgtctac cctgctgcat cgctcctaga aatttgtgcg
     1561 catctttgta atgaaacccc actgcaacgt tattccagca cgataacccc tgatacgcat
     1621 tcaatgaatt caccgctcac aatcgaccta caagatgtaa aaggtcaggt tcaggccaaa
     1681 cgtgcgttgg aaattgcagc cgcagggaat cacagcctct tgatggtcgg cccgcctgga
     1741 actggcaaat cgatgctagc ggagcgactg ataacgatct taccaaccat gagtcagcaa
     1801 gaagcattag aaacagccgc gattcaatcg cttgctcaag gtcagtttga aatagcgcac
     1861 tggaaacgtc gtccttttcg gtcaccgcat cataccgctt cagcaaccgc catggttgga
     1921 ggcggtagca taccgcgccc cggtgaaatt tcactggcgc tgaatggcgt actctttctc
     1981 gatgaattac ccgaatttga tcgaaaagtc ctagaagtac tacgggagcc cctagaatca
     2041 ggccatatca ccatctcacg cgcagcgcgg caaactaatt tcccagcgcg ttttctatta
     2101 attgccgcca tgaacccctg cccctgtggc tatttaggtc atccttctgg aaaatgccac
     2161 tgtacacccg atcaagttgc acgctatcgg ggcaaaattt ctggcccgct gctcgatcgt
     2221 atcgacatgc aaatcgaggt tccttcgttg ccacagaatg atctgatgca atcgcagtca
     2281 atcggtgaga aaagtaacat tatccgcgaa cgtgttgaga aagcacacca attgcaatat
     2341 gaccgccagg gaaaaaccaa tagtcgcttg agtgtaaagg aaattgatcg actctgtgtg
     2401 ctggatacag ccagcgagaa tttattgaag caagcgatca atcgattaag cctatctgca
     2461 agagcctatc atcgcatttt gaaattggca cgcaccatcg cggatttggg gaataccgaa
     2521 aaaattaatc atcaacatat tgcggaagca attcagtatc gtagactgga taagcattaa
     2581 caactatctg gttaagatga actaaaaata acttaatgtg aaaacagtgc gttaaatccg
     2641 ttttttaaaa actgccctta aaacagtaaa atctgttaaa taaaacttgc tctattatcc
     2701 gatcttaatc cgcatcgcat tgattcaaac acagagaaaa tccaatgaat gatccagata
     2761 tccgctggca acaacggttt gccaattatc aaaaagcgtt gctgcaattg caaaatgcgg
     2821 ttgagctcag caatcaacgg gcactcagcc cattggaaaa acaaggtgtt attcaagcat
     2881 tcgaatttac ccacgaattg gcttggaatc tattgaaaga ttttttgcag gatcaaggca
     2941 atcagaatgt cagaggctca aaagacgcaa ctcgtgaagc atttaaagtt gagttgattc
     3001 aagatggcga gcaatggatg gccatgatcc agagccgcaa catctcctca catacctaca
     3061 atgagcacac cgctgagcag ttggtgaatg caatcattca tgattactat cctttattca
     3121 gggcactgaa aaaagaaata gaaaaatacc agccatgaac gagcagtatt catttggttt
     3181 gtcacatacc accttggaaa aactcaatcg cgtatttgca agacacgaca ccatcgattc
     3241 agtgttgatc tatggttcaa gagcgaaagg aaattatcgc cgaggctccg atatcgatct
     3301 gacaattaaa ggcagcgaga tttcatttgc cgagttcatg caaattgaag accagattga
     3361 cgacctgatg ttgccttata ccgtcgattt gtcgcaatac cgcctacttg aaaataccga
     3421 cctgatcgcc catatcgacc gagtaggacg cgttatttac acgcgaccga ggaaataact
     3481 gcgcactcag ttttggcaag tttttcgagc gtggatgctc tgacttgaaa ccatcggcct
     3541 cctccaacgt aaccattaca ccccctaaaa cacctaccta atccaaatcg ttttgatatt
     3601 cacgaactca cgaataccgt gataggacaa ttctctgcca tagccggaat ctttgatgcc
     3661 gccgaatggc aagcgtgggt cgcttttgac gataccattg acgaatgtgc aaccggcatg
     3721 aatttgccgt gccagtgctt caccgcgtgc cagatcgcct gtccatacac tgccaccgag
     3781 gccaaattgg gtttgattcg ctaattttat tgcttcctcg gcattactga cacgcgcaat
     3841 ggcgaagaca ggtccgaata attcttcgtc aaatgccggc attccaggtt gcacgtgatc
     3901 cagaatggtt ggtgcgtaat aggcgccctg ctcaccaatg gaaaaaccgc cggctcgtaa
     3961 ctttgcgcct aatgccaggc ttcgctctac ttgcgcatgc aattcgtcgc gcaaatccac
     4021 tcgtgccatc ggtgcaatgg tggttgcggc atctttgggg tcgccactgc gtaactcgct
     4081 gataagtgct tgcaaccgct ctacaaaacc atcagcgatc gtttccagca aaataaagcg
     4141 cttcgcagca atgcaacttt gccccatatt ttgcaaccgg gccattagtg cttgctgcgc
     4201 agtccattcg atatcagcat cgtcaagcac gataaacgga tctgatcctc ccaattccaa
     4261 cacacac
//
LOCUS       DAOQLJ010000177         4205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_10345_length_4205_cov_7.041687, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000177 DAOQLJ010000000
VERSION     DAOQLJ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10345_length_4205_cov_7.041687"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4205)
                     /locus_tag="PK522_10270"
     CDS             complement(<1..>4205)
                     /locus_tag="PK522_10270"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNC42157.1"
                     /translation="ASSKVGKAARERLKKKYGEAVYAFEHRTLTESPIQNALQLVKQL
                     PNNAKLHLITYSRGGLVGELLCLGDLKSTLPDTETIHNLFAADRTIALQLGLNPLDES
                     AQKERNKAYDDDREALQTLLKELKQRQIKVERFVRVACPASGTTLASGRLDRWLSALN
                     YLSGDGLFGDTVDFLLAVLKERTDPRTLPGLEAMMPGSALTRLLQDPSLETNADLSII
                     AGDIEGDSVWSQLKLWVTDWFYGSDHDLVVNTGSMYGGLRRLPQQARFLCDRGPTVHH
                     FNYFNNDKTINWLLQGLERLDGCNGPFQAIEHAKPEQPRWRSAVQRSRSSATPRPLAV
                     VLPGTMGSTLKVGKEEVWLNYWTLAQGGLKKIQMGAIGVQPDDLLDDFYGPLLEFLAL
                     THRVEIVPYDWRLSIREAAKRLIETLQEHLPEAERNYQPVHLIAHSMGGLVVRAMLAD
                     GKEGTALWQRIVKLPKSRFLMLGTPNQGSYEAVRWLTGNNATQAKLSLLDLTQNTDEI
                     ANLVRRYPGLLELLPFDPDSKDFTQSNLWADLKAQLSAAWLPPETLDLVNIRDTWSLL
                     KNPAQSVDPKYTCYVAGCQDATVVDYRLVDCGGWLHANSNCKRFEFLATREGDGTVSW
                     KSGRLPDVPLWYLEDTAHDDLCRQKRAFPGYLDLLMNGDTSHLSKTPPGRARAPVGEP
                     ELFALPAYPVIDSIPSEASLRNFGFGSSRPMREMESGLTIPIIEVSICHGNLAYAKHP
                     VLVGHYRGDGIFSAEKALDERLECQLSTRIRLGLYPGAIGSHALFYAKEPNAKPSGAI
                     VIGLGQVGDLTPGLLTLGVQNALLECALQIAQWPAEDTRFSTEAGKRSIDITSMLVGT
                     GAGGMTVRDSIEAILRGAIAANEKLAEAELVDQVAINRLEFLELYEDIAIAAAEALNL
                     ILANNQLSNHVNWPDATVRAGEGGYTRLRYDEMGGWWHRMEISETEGREMLRFVSTTD
                     RARAEETLATGQLKLADNFIRQASQDAGANSEAAKTLFEMLLPNRLKELAPQQEGLVL
                     IVDESSARYPWELLENRWSHNGKPPAVSAGLIRQLKTSVFRPSPVHTTKLNALVIGNP
                     NLSGWSSFPDLVGAREEAQKVSELFNGNGFQAIDYIDANADDILSGLHKDEWRVLHLA
                     GHGVHDFDVSKADSSALDLLDNATQSVYSKKKLISGMVIGKNTFLTPGDIEQMRWVPE
                     LVFINCCYLGKTQSVIEKPYGALAANLAAQFIVMGVKAVVSAGWAINDQAGLAFADSF
                     YQKMLLGMSFGDAVRLAREENWNNFKTLNTWGAYQCYGDPDYRLMNKGTDLLAPSTKK
                     KTYYAPAELLTDLKNDTAFIRMQLNDKRKTEAELSDLKQMIDGRLTAIPQDKQKAWLA
                     REDVKIAFGLAYGELRAWDEAIQ"
BASE COUNT         1104 a         1094 c          868 g         1139 t
ORIGIN      
        1 actgaatggc ttcatcccat gcacgtaatt caccataggc aagtccaaat gcaatcttca
       61 catcttcgcg ggccaaccag gccttttgct tatcttgagg aattgctgtt aatctaccat
      121 cgatcatttg ctttagatca ctcaattccg cttctgtttt gcgcttatca ttcaattgca
      181 tgcgaatgaa tgcggtatcg ttcttcaaat cggttaacaa ttctgcgggt gcataatagg
      241 tttttttctt agtggaaggt gctaataagt cagttccttt attcattaag cggtaatctg
      301 gatcaccata acattgatat gcaccccacg tattgagcgt tttaaaattg ttccaatttt
      361 cttcacgtgc aagcctaacg gcatcgccaa acgacatccc tagaagcatc ttctggtaaa
      421 aactgtctgc aaaagctaaa cctgcttgat cattgatcgc ccaccccgct gaaacgactg
      481 ctttaacgcc catgacaata aattgtgcgg ccaggtttgc ggccagggca ccatagggtt
      541 tctcaatcac cgattgggtt ttaccaagat aacaacagtt gatgaatact aattctggca
      601 cccaacgcat ttgctcgata tcgcctggtg tcaaaaatgt attttttcca atcaccatcc
      661 ctgagatcaa ttttttcttg ctatatactg attgagtagc gttatctagc agatctagcg
      721 cactggaatc tgccttgctg acgtcaaaat catgaacacc atgacctgcc aaatgaagca
      781 cacgccattc atctttatgc aaccctgaaa gaatatcgtc cgcattggca tcgatgtaat
      841 cgatggcttg aaaaccattc ccattgaata gttcactcac tttctgcgct tcttcgcgtg
      901 ccccaaccaa atcaggaaaa ctactccacc cagaaaggtt aggattacct attaccaaag
      961 cattgagctt tgtggtatgt acgggcgaag ggcgaaatac ggatgttttg agttggcgaa
     1021 ttaatccagc gctaacggcc ggtggtttgc cattatggct ccagcgattt tcgagtaatt
     1081 cccagggata acgcgctgaa gattcatcca cgattaaaac aagcccttcc tgttgtggcg
     1141 ctaattcttt aagacgattt ggcagcaaca tttcaaatag cgtctttgct gcttcactat
     1201 tggcacctgc atcctgggac gcttgccgaa taaaattatc agcaagtttg agctggcctg
     1261 ttgcaagggt ttcttctgca cgggcccgat ccgtggttga gacaaaccgc agcatttccc
     1321 gcccttcagt ttcgctaatt tccatccgat gccaccaacc gcccatttca tcatagcgta
     1381 ggcgagtata accaccctcc cccgctctca cggtagcgtc tggccaattg acatgattgg
     1441 ataattgatt attcgccaaa atcaaattga gtgcctcagc cgctgcaatt gctatatcct
     1501 catatagctc aagaaactcc aaacggttga tcgccacttg atcgactaac tcagcttctg
     1561 ctaatttctc gttggcagca atggcgccgc gaagaatcgc ttcgattgaa tcccgaacgg
     1621 tcatcccacc cgcgccagtc ccgaccagca tcgacgtgat atcaatagaa cgttttccag
     1681 cttcagtgct gaatcgggta tcttccgctg gccattgggc aatctgtaat gcacattcca
     1741 gtagcgcgtt ctggacacca agggtcaata atcccggcgt cagatctccc acttgcccca
     1801 acccaattac aatcgcccca ctgggtttgg cattcggttc tttggcataa aacaacgcat
     1861 ggctaccgat tgcaccggga tataatccta gccgaatccg ggttgataac tgacactcca
     1921 gacgctcatc caatgctttc tcagcgctaa aaatcccatc tccgcgataa tgccccacta
     1981 acacgggatg tttggcatag gctagattcc cgtgacaaat actgacttca atgatgggta
     2041 ttgtcagccc actttccatc tcacgcatcg gtcgtgacga tccaaaccca aagttacgca
     2101 aacttgcttc actgggaata ctatcaataa ccggataggc cggcagggca aacaattcag
     2161 gctcacccac tggagcacgc gcacgaccgg gtggagtttt tgatagatgc gaagtatctc
     2221 cattcatcaa caaatccagg taacccggaa aagcgcgttt ctgcctgcat aaatcatcat
     2281 gggcagtatc ttccaaatac cataggggaa catcgggtaa ccgacctgat ttccagctga
     2341 ctgttccatc tccttcccgg gtagccaaga actcaaaacg cttacaattt gaattggcat
     2401 gcagccatcc cccgcaatcg actaaccgat aatccactac ggttgcatcc tggcatccag
     2461 caacataaca ggtatatttt ggatcaaccg actgcgctgg attcttaagc aaagaccaag
     2521 tatcgcgaat attgacgaga tcaagtgttt ccggtggcag ccatgctgcc gataattgcg
     2581 ctttaagatc agcccaaaga ttcgattggg taaaatcttt actgtctgga tcaaacggta
     2641 acaattctaa taaacccgga tagcgccgta ctagattggc aatctcatcc gtattttgcg
     2701 tcagatcgag caagctcagc ttagcctgtg tcgcattatt gccagtcaac cagcgcacgg
     2761 cttcatacga accctgattc ggtgttccta gcattaggaa gcgacttttg ggtaatttaa
     2821 caatgcgctg ccataacgca gtgccttcct tgccatccgc tagcatcgct cttacaacca
     2881 agcctcccat agaatgggca atcagatgta ctggttgata atttcgttcc gcttcgggta
     2941 aatgttcttg caatgtttcg ataaggcgtt tcgccgcttc tcggattgaa agacgccaat
     3001 cataaggaac gatttcaacg cgatgcgtca gcgccaaaaa ctcaagcaat ggaccataaa
     3061 aatcatctaa taaatcatcg ggctgaactc ctatcgcacc catctgtatt ttttttaatc
     3121 caccctgtgc aagcgtccaa taatttaacc atacttcttc ttttccaacc tttagggtgc
     3181 ttcccatcgt ccctggcaat acaacagcca ggggacgtgg cgttgcagaa ctacggctgc
     3241 gttgcactgc agatcgccaa cgcggttgtt ctggcttagc gtgttcaata gcctggaatg
     3301 ggccattaca accatcgaga cgttccaatc cctgcagcaa ccagttaatc gttttatcgt
     3361 tgttaaaata gttgaaatga tgaacggttg ggccacgatc acagagaaag cgggcttgtt
     3421 ggggtaagcg acggagccct ccatacatgg aaccggtatt cactactaaa tcatgatcac
     3481 tgccataaaa ccagtcagtc acccatagtt ttagctgact ccataccgaa tcaccctcaa
     3541 tatcgcccgc aataatagaa agatccgcat tggtttctaa cgatggatct tggagtaagc
     3601 gcgtcaatgc agaaccgggc atcatcgctt ctaacccggg cagtgttcgg ggatcagtac
     3661 gttccttaag gaccgccagt aaaaaatcga cagtatcgcc aaacaatcca tccccggata
     3721 gataatttaa cgccgataac cagcgatcga gacgaccgga agctaatgtc gttccacttg
     3781 cgggacacgc cacacgcaca aaccgttcaa ctttgatttg cctttgctta agttctttta
     3841 gtaacgtttg cagtgcttcg cgatcatcgt cataagcttt gtttctttct ttttgcgcgc
     3901 tttcatccaa aggatttaat ccaagctgta acgcgatggt acgatcggct gcaaatagat
     3961 tatggattgt ttctgtatcg ggtaaggttg attttaaatc ccccaaacaa agtagctcac
     4021 ccaccaatcc accccgtgag taagtaatca aatggagttt tgcattattt ggaagttgtt
     4081 tgactaattg cagcgcattc tggatagggc tttctgttaa tgtacggtgt tcaaaagcgt
     4141 atactgcttc gccatatttt ttcttcaatc tctcccgcgc tgctttacct acttttgaag
     4201 acgct
//
LOCUS       DAOQLJ010000178         4185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_10417_length_4185_cov_5.250363, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000178 DAOQLJ010000000
VERSION     DAOQLJ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10417_length_4185_cov_5.250363"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PK522_10275"
     CDS             complement(<1..634)
                     /locus_tag="PK522_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNC42158.1"
                     /translation="MIPGRIVAAVLAVIVLPANAQGKEDILNKAGTAIFAQSATKATL
                     TASNSHNVTLLPSLIAEALENNPEIQAAYQEREAAQQRVSPAEALDDPMLEAGVINAP
                     LASSPFNREDMTMKMIGLSQRLPFPGKRGLRKDVAAKDAEAIEQGYHETVNRVVHDLK
                     TAYFDLGLTLEMIKLVEKNKQTLERFLRIAEERYQVGQGSQADALKAQTQV"
     gene            complement(631..1053)
                     /locus_tag="PK522_10280"
     CDS             complement(631..1053)
                     /locus_tag="PK522_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HNC42159.1"
                     /translation="MSLSFSFKLLLSLIFACMSALSLADRITQPIPIDSVSDITDNTS
                     NNSAQISNKESSVSSNLIFARGVVLQVDQSNATIKINHDPIAALNWPRMTMPFRLKER
                     SLATKIKEGDIVEFSLEKEASNYVIVKLSKQNSTGSSQ"
     gene            complement(1207..1587)
                     /locus_tag="PK522_10285"
     CDS             complement(1207..1587)
                     /locus_tag="PK522_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42160.1"
                     /translation="MKRRNYKWLLVFFMLWLPLQGAAAAALSVCVQENNINSHHNIST
                     KAIDSHHHDACHKQTADNTTDHLLASLPCDDTSCNAYSNTPILSGYTAVMQTNDTSAI
                     TSLSPSFISFVPEQPQHPPLANPL"
     gene            complement(1667..2023)
                     /locus_tag="PK522_10290"
     CDS             complement(1667..2023)
                     /locus_tag="PK522_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42161.1"
                     /translation="MKNKILILIAVGLLSLLASCAQMNPHPMDMTKAIQEAKSPTDHN
                     ALAKHYEDAAKEMLSKAQEHKKMLEGYESNSSHYGRQALDLQAHCRNLINAYEQAAKA
                     NTDMADSHRKMAEEIK"
     gene            complement(2467..2925)
                     /locus_tag="PK522_10295"
     CDS             complement(2467..2925)
                     /locus_tag="PK522_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbN"
                     /protein_id="HNC42162.1"
                     /translation="MMDLPPDMQERVVCSITAAIKYEIPANILLAIAEKEGGKPGQWV
                     RNSNGTHDVGSMQFNTLFLQDLSKYGIKPDHVANTGCYAYDLAAWRVRLHIKNDKGDI
                     WTRAANYHSRTPKYNAKYRTDLIVRAVKWANWLENRFITENNKIPGVYMD"
     gene            complement(2957..4045)
                     /gene="trbL"
                     /locus_tag="PK522_10300"
     CDS             complement(2957..4045)
                     /gene="trbL"
                     /locus_tag="PK522_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HNC42163.1"
                     /translation="MFRTPITFFCLFFLLFFSAGAFAELAYIDPATNQSVLDQVVNKF
                     QGKVKSWQNIIQAAAERLFWTLVLISMVWTFGMMLLRKADIGDFFAEFTRFIIFTGFY
                     FWLLTNAVSGHNIAGTIIDSMQQLGGTAAGLPSGASHSSIMDIGVLIWNQATNNLTFM
                     QPMDSLMGFVISLIILIILAVIAVNMLLLLISSWVLMYAGIFFLGFGGARWTSDIAIN
                     YFKTVLGVGIQLFVMLLIVGIGSDLLSSFYAKMGKNVLNYEELAVMLIFTIAFFVLIS
                     RLPLLLAGIITGSSIGSTVGIGSYGAGGFMAAAGTAAAVASYGGTLAAGRLAAARSAG
                     PDALRSAIEKGQAQESAGMNNISQGGGK"
BASE COUNT         1201 a          925 c          814 g         1245 t
ORIGIN      
        1 atacttgtgt ttgtgctttc agcgcatccg cctgactgcc ttgtccaact tggtagcgtt
       61 cttcagcaat gcgcagaaaa cgttcgagtg tttgtttatt tttttcaacc aatttgatca
      121 tttccagcgt aagcccgaga tcaaagtaag ctgttttaag atcgtgcaca acgcgattaa
      181 ccgtttcatg atagccctgc tcaatggctt cggcatcctt ggcggcaaca tctttacgca
      241 agccgcgctt tcccggaaaa ggcaaacgct gggatagacc gatcattttc atggtcatat
      301 cctcgcggtt gaatggtgaa gatgccaacg gtgcattgat tacgccagcc tcaagcatgg
      361 ggtcatccag cgcttccgct ggtgatactc tctgctgagc agcttcacgt tcttgatacg
      421 cagcctgaat ctccggatta ttttccagtg cttctgcaat caaacttggg agcagcgtca
      481 cgttgtggga gttggatgct gttaaagttg cctttgtagc agactgtgca aaaattgctg
      541 taccagcctt gtttaaaata tcttccttac cctgagcatt tgccggaagt acgataacag
      601 caagcactgc cgcaacaatt ctgccgggaa tcactgcgaa ctcccggtgg aattttgctt
      661 actcaatttt acgatgacgt aattggaagc ttctttttca agagagaatt caacgatatc
      721 gccttcttta attttagtag ccaatgagcg ttccttcagg cgaaagggca tagtcattct
      781 tggccaattt agtgcagcga tcgggtcatg atttattttt attgtggcgt tggattgatc
      841 gacctgcagc accacacccc ttgcaaaaat gagattggaa gaaacagacg attctttatt
      901 ggatatttgt gccgagttgt tgcttgtgtt atcagtaatg tcggatactg agtcaattgg
      961 aattggttgt gtaatccgat ctgcaagtga cagagcagac atacaggcaa agattaacga
     1021 cagtaataat ttaaaagaaa atgaaaggga catactaacc tcctaactgt tgtatcacca
     1081 aaacttatca gtctagtaat agacaataat ggcaatagct tgcgcattgc aaaatcttgc
     1141 gtagcaatta cttaaacgca ggattaatct gaattgatat tttttgcctg gattacaggc
     1201 ggatttttaa agggggttag ctagcggagg atgttgcggc tgttcgggta caaatgaaat
     1261 aaaacttgga ctcaatgaag taatagcgga agtgtcgtta gtttgcatga cagcagtgta
     1321 gccagacagg atcggcgtat tactgtatgc gttacaagag gtatcatcgc acggcagact
     1381 tgctaacaaa tgatcggttg tattatctgc tgtttgctta tgacaagcat catgatgatg
     1441 gctgtcgata gctttcgtcg atatgttgtg atgactgttg atattgtttt cttgcacaca
     1501 cactgacaaa gcagccgctg cagcgccttg caatggaagc cacagcataa aaaaaaccaa
     1561 tagccatttg tagttgcgtc ttttcatttg tttattataa acaatttcaa gcttatcact
     1621 agacaaaaat taggtagctc aatcgtgctg cttcatcaat cgaatattac ttaatctctt
     1681 cagccatttt gcgatgggaa tcggccatat ccgtatttgc tttcgcagct tgctcgtatg
     1741 cattaattag gtttcgacag tgtgcctgca aatctaacgc ttgtctgcca tagtgggaag
     1801 aatttgactc gtatccttca agcattttct tatgttcttg tgcttttgac agcatttctt
     1861 ttgcagcatc ttcataatgc ttagctagtg cattatggtc agtaggactt tttgcttcct
     1921 gaatagcttt agtcatatcc attgggtgtg gattcatttg tgcacatgaa gctaataaac
     1981 ttaataaacc aacagcaatt aaaattaaga ttttgttttt cacaatagtt ctccataata
     2041 aaaattaata tagcccaata gaaccataat actactatag ttatagatct ttgcacgaca
     2101 aaaagtaaga caaagttgat gaatgatcta atttaggtgg actcaaggat gaagtggaat
     2161 tttattatga gcttttcaga ttttgatgtc gcccaccgca cgcgtaaaga tctcatatcg
     2221 taactaccta atttataacc tcgagcttta gtaattttct ctgctttcca tacccccatt
     2281 attttcgttc tgaaggattc tctgtacatt tataacgatc aatttttttg tcagtttatt
     2341 aatcttaatc gcaggatcgc ctttaacttt aattaaggca taagtattta acattaccta
     2401 tacgtaacat aagtctttta ttcacagcct tttccacgaa atttgtggac aacgcataga
     2461 actgacttaa tccatataaa cgccaggtat tttattgttt tccgtgataa aacggttttc
     2521 tagccaattc gcccatttaa cagccctgac aatcaaatcg gtacgatact ttgcattata
     2581 ttttggagta cgcgaatgat aattggctgc tctcgtccaa atatcgcctt tatcattttt
     2641 gatatgtaaa cgcacacgcc atgcggccag atcataggca taacaacccg tatttgctac
     2701 gtgatcaggc ttaatgccat atttggataa atcttgaaga aacaaagtat taaactgcat
     2761 cgagccaaca tcgtgagtac cgttactatt acgcacccac tgacccggtt taccgccttc
     2821 tttttcggct atcgccagta agatattggc tggtatttca tacttaatgg ctgctgtaat
     2881 tgagcacacc accctttctt gcatatccgg cggtaaatcc atcatcaaac gaattgctta
     2941 aatgcctcct tatccattat ttaccccctc cttgcgaaat attgttcata cccgcagatt
     3001 cctgagcctg acccttttca atagcgctac gtaatgcatc cggccccgca cttcttgccg
     3061 ctgctagtcg accggctgcc agcgtgccac cataagacgc aacagctgcc gccgtacctg
     3121 ccgctgccat aaaaccaccg gcgccataac tgccaatccc gacagtcgaa cctatgctac
     3181 tacccgttat aatgccagcc agcaacagcg gcaatcttga aatcaataca aaaaatgcaa
     3241 tcgtaaaaat tagcataacc gccagctctt cataattcag aacgttttta cccattttgg
     3301 cgtaaaaact ggataacagg tcactaccga taccaacgat caggagcatt acaaatagct
     3361 gaataccgac acccagaact gttttaaagt agtttatggc gatgtcggat gtccaacgtg
     3421 cgccgccaaa gcctagaaag aatattcctg catacattaa cacccaggac gagatcaaca
     3481 agagcaacat gttaactgca ataacagcca gaatgattag aataatcaaa gaaatgacaa
     3541 aacccatcag actatccata ggttgcataa atgtaagatt attcgttgcc tggttccaga
     3601 ttaaaacacc tatatccata atactggaat gactggcacc actcggcaaa ccggcggcag
     3661 ttccacctaa ttgctgcatc gaatctatga tagtgcctgc aatattgtgt cctgatacgg
     3721 cattggtcag cagccagaag taaaaccctg taaaaataat aaaacgagtg aattcagcaa
     3781 agaaatctcc tatgtcggct ttacgcagca gcatcatgcc gaacgtccag accattgaaa
     3841 tcagcaccaa cgtccagaac aaacgctctg ctgcagcctg gataatgttt tgccaggatt
     3901 taactttgcc ttgaaactta ttaacaacct gatccagaac actttgattg gttgctggat
     3961 caatataggc tagttcagca aatgctccag cagaaaagaa caatagaaaa aataggcaga
     4021 aaaaggtaat tggtgttctg aacatcttat ttactccctt actcatacgg cacacaatta
     4081 ttcttgcgat ccattcgtag gttgttgaga tattggtgtg tcaaaccaac tctttctttc
     4141 cggcatttca cttaattctt tcttaggctc cttcaattca agctt
//
LOCUS       DAOQLJ010000179         4148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_10573_length_4148_cov_6.576839, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000179 DAOQLJ010000000
VERSION     DAOQLJ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10573_length_4148_cov_6.576839"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PK522_10305"
     CDS             complement(<1..270)
                     /locus_tag="PK522_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42164.1"
                     /translation="MKILIIGLSLLVGCSFSSNKPTSLALYDLGLKPVSEVQVAPVDV
                     SIMMTEITAPIWLTSPAMQYRLTYHDPARTYHYAHSRWVTAPAKLL"
     gene            complement(280..1170)
                     /locus_tag="PK522_10310"
     CDS             complement(280..1170)
                     /locus_tag="PK522_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNC42165.1"
                     /translation="MENRAHALAAGIFVIILGVATGYAVRWFSNQDILRDRYLLVSEA
                     GSVSGLNPAAVVRYRGVSIGKVEEIRFDTQDQTFILVGIAVNSDIKLPTNIYAQLANQ
                     GLTGLAYIELNTEGDPEETYLPSNARIELRASFIRKLSDSVENILNNTNKAVKRLNAL
                     MNQKNQEQFSAILANLAQAIQRYGSLVEQLQPGLKSLPTLSAEANRVMKQTHQLLAEV
                     NQTIVKTNQPGGLSDGLTQSVQAFADSLPKLREATESITQSTHHINRLLQRLEEHPQS
                     LLFGAPIATPGPGETGFHSP"
     gene            complement(1295..1558)
                     /locus_tag="PK522_10315"
     CDS             complement(1295..1558)
                     /locus_tag="PK522_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42166.1"
                     /translation="MKLVSYVVLITLGLTLATVHATETVPEKVEATSKELKREAKKTV
                     NRAEEALCTESEAECLKRKMKNRTEETWDTLKDKATDLKNKLD"
     gene            complement(1663..1956)
                     /locus_tag="PK522_10320"
     CDS             complement(1663..1956)
                     /locus_tag="PK522_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42167.1"
                     /translation="MLPEDKKAYLQKITDQLTEWEAKIDTLKAKGEHLAEEAKQEFEE
                     QMAEMNEKRAELSAYLDELTDKADDLWEDVKEEAEEKWDQISATVDHFISKFK"
     gene            2194..>4148
                     /locus_tag="PK522_10325"
     CDS             2194..>4148
                     /locus_tag="PK522_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNC42168.1"
                     /translation="MFLSLLLFLFTLIAILVAILVVPFLRRHLISRFVLKFYNKILPP
                     ISQTEREALDAGTVWWEGELFSGKPDWDKLLAYPKPELTPEEKAFLEGPVEELCAMLN
                     EWQITHELQDLPPHVWQFIKDNGFFGLIIPKSYGGYGFSALAHSEVVMKISSRSGTAA
                     VTVMVPNSLGPAELLLHYGTEEQKNYYLPRLAKGIEVPCFALTGPDAGSDAGSIPDFG
                     IVCRSEYNGQSDVLGMKVTWEKRYITLGPVATLLGLAFRLRDPDHLLGGEVDLGITLA
                     LIPTNTPGVNIGRRHFPLNGAFQNGPNSGKDVFIPMEWIIGGQERVGQGWRMLMNSLA
                     AGRSISLPATSTGAAKLAARTSGAYGRVRVQFKTPVGFFEGVEEALARIAGNTYMMDA
                     ARIMTAGAVDLGEKPSVISAIVKYHLTERSRQAINDAMDIHGGKGICMGPSNYLARTY
                     QQIPVGITVEGANILTRTMIIFGQGAIRGHPYVLQEINAAHLADKKKGLIAFDHALAG
                     HVIFSIRNVYRSLLLGIGGKYLKNHPDSALADTIPYYQQLTRFSAAFALLTDIAMITL
                     GGSLKRRERLSARLGDILSMLYLCSATLKKFENDGQPAADLPLLHWSIQDALYRTQQA
                     FDEFLRNFPGHFLIRIWLRWQVFPLGKR"
BASE COUNT         1068 a          953 c          905 g         1222 t
ORIGIN      
        1 caaaagtttt gccggtgcag taacccagcg actatgggca taatgataag tccgtgctgg
       61 atcgtgatac gtcaagcggt attgcatagc agggcttgtc agccaaatgg gtgcagtgat
      121 ttcagtcatc atgatcgata catcgaccgg ggcaacttga acttcggaaa ccggctttag
      181 acccagatca tacaaggcca gtgatgtggg tttattgctg gaaaacgaac aaccaaccag
      241 cagactcaaa ccaataataa gaattttcat gaaaacgatt tacggtgaat gaaaccccgt
      301 ttcaccgggc cctggtgttg caatgggcgc gccgaacaaa aggctttgtg ggtgctcttc
      361 aagtctttgc agtaaccgat tgatatgatg cgtactctgc gtgatcgact cagttgcctc
      421 acgtaattta ggtaatgaat cagcaaatgc ctgcacactt tgcgtcaaac catcgctcaa
      481 cccccccggt tggttggtct taacaattgt ttggttgact tcagccagca attgatgcgt
      541 ttgcttcatg acgcgattgg cttccgcgga tagcgtaggc aaagatttta atccaggctg
      601 cagttgttca accaaacttc cgtagcgttg aatcgcttgc gccaaattag ctagaatagc
      661 gctgaattgc tcctgattct tctgattcat caacgcattg aggcgtttca cagctttatt
      721 cgtattgttc aaaatatttt caaccgaatc ggaaagtttg cggataaacg aggcacgcag
      781 ctcaatacgc gcattggatg gcaaataagt ctcttctgga tcaccttcag tattcagttc
      841 aatgtacgca agccccgtca atccctgatt agctaattgt gcataaatat ttgttggcaa
      901 tttaatgtcg ctgttcacgg caatgccaac gagaatgaag gtttggtcct gagtatcaaa
      961 ccgaatttcc tcgactttac caatcgatac tcctcggtaa cgtacaacgg ctgctggatt
     1021 caggccggat accgaccctg cctccgatac caataaataa cgatcacgca gaatatcttg
     1081 gttgctaaac caacgaaccg cataacccgt cgcaactcca agaataatca cgaagattcc
     1141 ggctgcgagt gcatgagcac gattttccat ttgctaattt aaattgaggt agatcaaatt
     1201 aattgggtcg agatacggtc gagcgcgaat tcagatgcca atacggaaat cattttaatt
     1261 acaataagat ttgatagttt atcagctaat ggctttaatc cagtttattc ttaagatcgg
     1321 tcgctttatc ttttaacgta tcccaggttt cttctgtgcg gtttttcatc ttgcgcttca
     1381 aacattcggc ttcactttca gtgcacaagg cttcttcagc acggtttacc gtttttttgg
     1441 cctcacgctt caattctttc gatgtggctt ctactttttc tggaacggtt tcggttgcat
     1501 gtaccgttgc tagggttaac cctagtgtaa ttaaaaccac ataggaaacg agcttcatca
     1561 tagatctcca ataatagatt gagtaagaat actgctgtac attaaacaaa ctgctagtgc
     1621 aacagatgat ttattagaat cgtctgctgc aaaattagta gcctatttga acttagaaat
     1681 aaaatgatca acagttgcgc taatttgatc ccatttctct tctgcttctt ctttgacatc
     1741 ttcccacaga tcgtctgcct tatccgtcaa ctcatccaaa taagccgata attcggcccg
     1801 cttctcattc atttccgcca tttgttcttc aaattcttgt ttcgcttcct ctgcaaggtg
     1861 ctcacctttt gccttgaggg tatcgatttt ggcttcccat tccgtcagct gatcagtaat
     1921 tttttgcaag taagcttttt tatcttcagg taacatcgat atctcctttc tcgttaagct
     1981 tcacaaataa aaacaggttg ttgcagctgt attatttcta acctatgtta aatttttgtg
     2041 agccccagcg caattaattc tcaattgatt ttaatcagta gcaagggttt attacaaaat
     2101 tttggtaatt tcttatttgc aaggcttgac ataagcattt tctacggtac tgtatccagt
     2161 gtcattacac ttactttttt tacaggtaaa actatgttcc tgtcacttct gctatttttg
     2221 tttacactga ttgcaattct cgtcgccatt ctcgttgttc cttttttgcg acgccatttg
     2281 atttcacgat ttgttctaaa attctataac aaaatccttc cacccatttc acaaaccgaa
     2341 cgcgaagccc tcgacgctgg aaccgtttgg tgggaagggg aattatttag tggaaagcca
     2401 gattgggata aattgctggc ttaccctaaa ccggaattaa cgcctgagga aaaagcgttt
     2461 ctcgaaggtc ccgtcgaaga actctgtgcc atgctgaacg agtggcaaat tacgcatgaa
     2521 ttgcaagatt taccgcccca cgtatggcaa ttcatcaaag acaacggttt ttttggacta
     2581 atcatcccta aatcgtacgg tggttatgga ttctctgcgc ttgcccactc cgaagtcgtc
     2641 atgaaaatca gctctcgcag tggtacggct gcagtaaccg ttatggtacc gaattcattg
     2701 ggccccgctg agttattgtt acattatggc accgaggaac agaaaaacta ttatctaccc
     2761 cgcctcgcca aaggaatcga ggttccgtgc tttgcgttga ccggaccgga tgctggttct
     2821 gatgctggtt cgattcctga ttttggaatc gtttgccgca gtgaatataa tggtcaatct
     2881 gacgttcttg gcatgaaagt aacttgggaa aaacgctaca ttacactcgg cccggttgcg
     2941 acgctgcttg gattagcttt ccgattacgt gatccagatc atttgttagg aggtgaagtt
     3001 gatctcggta ttacccttgc gttgattcca acgaataccc caggcgtcaa tattggtcgc
     3061 cgccattttc cattgaatgg cgctttccaa aatggaccga attcaggtaa agatgttttc
     3121 attcccatgg aatggatcat tggtggtcaa gagcgggtcg gtcaaggatg gcgcatgtta
     3181 atgaacagct tggcagcagg tcgttcgatt tcattacccg ccaccagtac cggtgctgct
     3241 aaattagctg cgcgcaccag cggggcttat ggacgagtac gcgtacaatt taagacgccg
     3301 gttggcttct tcgaaggggt agaagaagcg ctcgcacgta ttgccggtaa tacttatatg
     3361 atggatgctg ctcgtatcat gacggccgga gcggttgatc tcggtgagaa accttctgtg
     3421 atctcagcaa tcgtcaaata ccacctgacg gaacgcagcc ggcaagcgat caacgacgcc
     3481 atggatattc atggcggaaa agggatttgc atgggaccga gcaattacct ggcacggacc
     3541 tatcaacaaa tcccagtggg tattaccgtt gaaggtgcaa atattttgac gcgcactatg
     3601 atcatctttg gtcaaggcgc tatccgcggt catccttatg tgctacaaga aatcaatgca
     3661 gcccatctcg cagacaaaaa gaaagggtta atcgcttttg atcatgcgtt ggcgggccat
     3721 gtgattttca gcattcggaa tgtttaccgc agcttgctgc tcggtatcgg gggtaagtat
     3781 ctaaaaaacc acccggatag tgcgctagcc gatactatcc cgtattacca gcaactaact
     3841 cgtttctcag cggcttttgc attattaact gatatcgcaa tgataacgct gggcggctcg
     3901 ctcaaacgca gagaacgact gtcagctagg ctaggcgata tcttaagcat gctttattta
     3961 tgttcagcca ctttgaagaa atttgaaaat gacggtcaac ctgccgccga cttaccgttg
     4021 ttacactggt cgattcaaga tgcgctatat cgaacccaac aagcattcga tgagttctta
     4081 cgtaattttc ccggccattt tcttattcgg atttggctca gatggcaagt tttcccgctt
     4141 ggcaaacg
//
LOCUS       DAOQLJ010000180         4127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_10682_length_4127_cov_5.752701, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000180 DAOQLJ010000000
VERSION     DAOQLJ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10682_length_4127_cov_5.752701"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1564
                     /gene="thiC"
                     /locus_tag="PK522_10330"
     CDS             <1..1564
                     /gene="thiC"
                     /locus_tag="PK522_10330"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNC42169.1"
                     /translation="SQERLNNPKLSELRFNLKRASRRAKSGTNVTQMHYARQGIITPE
                     MEFIAIRENQHWEALKNNNQQDLIRQHPGQDYGAAIPRLITPEFVRDEVARGRAIIPA
                     NINHPESEPMIIGRNFLVKINANIGNSALGSSIQEEVEKMTWAIRWGGDTVMDLSTGK
                     NIHETREWIIRNSPVPIGTVPIYQALEKVDGKAEELTWEIFRDTLIEQAEQGVDYFTI
                     HAGVRLPFIPLTAKRMTGIVSRGGSIMAKWCLAHHKESFLYTHFEEICEIMRAYDVSF
                     SLGDGLRPGSIYDANDEAQFAELKTLGELTQIAWTHDVQVMIEGPGHVPMHLIKENMD
                     LQLKYCQEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPD
                     KDDVKDGIIAYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWSDQFNLSLDPDKAKQ
                     FHDETLPQEGAKLAHFCSMCGPSFCSMKITQDVRNYAAQQGIAEDEALKHGMAQKSSE
                     FKEKGAEIYGKL"
     gene            1591..2412
                     /locus_tag="PK522_10335"
     CDS             1591..2412
                     /locus_tag="PK522_10335"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNC42170.1"
                     /translation="MGWALLLKALILGLVEGLTEFLPVSSTGHLILIGDLLNFNDEKA
                     KVFTVAIQLGAILAVCWEYRERLKTAVAGLGSPSSNRFFLNLLVAFLPAAILGFFFIK
                     TIKFYLFHPLPVALALIIGGILILWAERRKHVVEVDQVEAMDWKHALKIGCAQCLALI
                     PGTSRSGATIIGGLFFGLSRKAAAEFSFFLAIPVMFAATFYDVYKNRDLLILDDLTLF
                     AVGFIAAFLSAWLAIKGLMRYISNHDFTIFAWYRIVFGLVILTTAYSGVITWSSY"
     gene            complement(2434..2886)
                     /locus_tag="PK522_10340"
     CDS             complement(2434..2886)
                     /locus_tag="PK522_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC42171.1"
                     /translation="MEDTTAILEKAHQRAEEMDLPYKGALTPAEALAILQTHPNAKLI
                     DVRCKAELDWVGRVPGAVEIELRTYPGMQPNPNFVDQVADRVDRNDLILFLCRSGGRS
                     DHAATIVAQSDFPNCYNILEGFEGDKDETGHRGTSGGWKAAGLPWVQS"
     gene            3315..3623
                     /locus_tag="PK522_10345"
     CDS             3315..3623
                     /locus_tag="PK522_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC42172.1"
                     /translation="MRKLNIALTLSALFVLTACGGSRDYSPPADASGEQIFSTACTEC
                     HKPLSANVAMILSEKVANKDAIIKKFQGGSMRMPAFKNIQGESADRLAEYILANSEVK
                     "
     gene            complement(3927..>4127)
                     /locus_tag="PK522_10350"
     CDS             complement(3927..>4127)
                     /locus_tag="PK522_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNC42173.1"
                     /translation="FAIEIYNTIQRSLIYPEAWPILQGDIRRCLVRRFPYGVLYSIEP
                     NGLYILAVMNLHRNPNYWENRK"
BASE COUNT         1136 a          835 c          966 g         1190 t
ORIGIN      
        1 tagtcaagaa cgattaaata atcccaaatt atcagaattg cgcttcaatt tgaaacgcgc
       61 ttcgcgcaga gcaaaatctg gaacgaatgt cacgcagatg cattatgctc gacaagggat
      121 tattacacct gaaatggaat ttattgctat tagggagaat cagcattggg aggcattgaa
      181 aaataataat cagcaagatc tgattcgcca acacccaggc caagattatg gtgcagcgat
      241 tccacgcctg attacccctg agtttgttcg cgatgaagtg gcgcgtggca gagcaatcat
      301 tccagccaat atcaatcacc ctgaatctga accgatgatt atcgggcgta atttcctggt
      361 taaaatcaat gccaatattg gaaattctgc gctgggctcg agcattcaag aagaagtaga
      421 aaaaatgact tgggcgattc gctggggtgg agatacggtg atggatcttt cgacgggtaa
      481 gaatattcat gaaactcgcg agtggattat tcggaatagc ccagtgccga tcggtacggt
      541 tcccatttat caggccttag aaaaagtcga tggtaaggca gaagaactta cttgggagat
      601 tttccgagat accttgattg agcaggctga gcaaggtgtg gattatttta cgatccatgc
      661 tggagtacgg ttgcctttta taccgctcac tgcaaagcgc atgacgggaa ttgtttctcg
      721 gggcggctcc attatggcca aatggtgctt agcgcatcat aaagaaagtt tcttgtatac
      781 gcattttgaa gaaatttgcg agatcatgag agcttatgat gtgagctttt cactgggtga
      841 tggtttacgt ccaggctcga tttatgatgc caatgatgaa gcacaatttg ctgaactaaa
      901 gacgctaggt gaattgactc aaatcgcctg gacgcatgat gtgcaagtaa tgatcgaagg
      961 ccctggccat gttcccatgc atttgatcaa agagaacatg gatttacagc ttaaatattg
     1021 tcaagaagca cctttttaca cgctaggccc actcactacg gatatcgctc ctggctatga
     1081 tcatattacc tccgcgattg gtgcagcaat gatcggctgg tatggtacag ccatgctgtg
     1141 ttacgtgacg cccaaggaac atttggggtt accggataaa gatgacgtca aggatggaat
     1201 tatcgcttac aaaattgctg cccatgcggc agatctggct aaaggacatc ccggtgcaca
     1261 aatacgggat aacgcgcttt ccaaagcgcg gtttgaattt cgctggagtg atcagttcaa
     1321 tttgagcctt gatcctgata aagcaaaaca gtttcacgat gaaacgctac cgcaagaggg
     1381 ggcaaaattg gctcattttt gttctatgtg tggcccaagc ttttgctcga tgaaaattac
     1441 ccaagatgtg cggaattatg ctgctcaaca aggcatcgca gaagatgaag cacttaagca
     1501 cggtatggct cagaaatcga gtgaattcaa agaaaaaggg gctgaaattt atggaaagct
     1561 gtgagcaatt tttctgctta gcataaaatc atgggatggg cgctactact taaagcattg
     1621 attcttggcc tcgttgaggg gttaaccgag tttcttcccg tttcctcaac aggtcatttg
     1681 atcttgattg gggatctact gaattttaat gatgaaaaag ccaaggtttt taccgtagcg
     1741 attcagcttg gcgctattct tgcggtgtgt tgggaatatc gagagagact caaaacagcg
     1801 gtggctggat tgggttcgcc ctcatctaat cgtttctttc tgaatttgtt ggttgcattt
     1861 ttgccagccg cgattctcgg ttttttcttt atcaaaacca ttaagtttta cctctttcat
     1921 cctctacccg ttgcgttagc gttaattatc ggtggaatct taattttatg ggctgaacgc
     1981 agaaagcacg tcgtcgaggt tgatcaggtt gaagcgatgg actggaaaca cgcgcttaaa
     2041 atcggttgtg cgcaatgctt ggcattaata ccaggaactt cgcggtccgg tgcgacgatt
     2101 atcggggggc tattttttgg tttatcgcgc aaagcagccg ctgaattttc tttttttctg
     2161 gctattcctg ttatgtttgc ggctactttt tacgatgtgt acaaaaatcg tgatttactg
     2221 atcctcgatg atttaaccct gtttgcagtg ggttttattg ctgcattttt gagtgcatgg
     2281 ctagccatca agggattaat gcgctatatc agtaaccacg acttcaccat ttttgcttgg
     2341 tatcgcatcg tatttggcct ggttattttg acgacagcct attcgggcgt gattacttgg
     2401 tcaagttact agcgcaataa acctatcaag aagttagctt tgcacccaag gtaatcctgc
     2461 cgctttccag ccccctgatg ttccccgatg gccggtttca tctttatcgc cttcaaagcc
     2521 ctctagaata ttgtaacaat tggggaaatc ggattgtgca acgatggttg cagcatgatc
     2581 ggatcttccg ccactccggc acagaaatag gatgagatca tttcgatcga cgcgatctgc
     2641 aacttgatca acaaaattag gattcggttg catgccggga taggttctaa gctcgatctc
     2701 aaccgcccca ggaactctac cgacccaatc cagctcagct ttgcaacgga catcgatgag
     2761 tttggcgtta gggtgagttt gtagtatcgc taaggcttct gcaggcgtta atgcgccttt
     2821 gtaaggcaaa tccatttctt cagcgcgttg gtgcgctttt tctaatattg ctgttgtgtc
     2881 ttccatttgt ttgtcacgtt aagggatatt agtaaattaa gccaataagt ttaattcaat
     2941 gttagcgtag ttcacaaata ataatactga ctgaggaaac ggaggctata ttgaaacaat
     3001 taaccattgc tgtctgggtc ttcgttaggt aggctggcgc atcaaattag tgggactcga
     3061 tagattttct ggaagtcgtg ccatgttttt ggctttatcg ttgttctatc ttcagaaagt
     3121 attggagttg ttcatagaca atcaggatct atcgagtcta gcgctttcga ttctcgcgtt
     3181 tgactagata tccctttggt tggtgggaag gtggaatcgt aagctcatca aaatttctga
     3241 tctgaaacta acgtaatgac ttacaattcg atagtatgat caagagagta aaattaataa
     3301 actggagtta gtaaatgaga aaattaaata ttgctttaac cctatcagca ttattcgttc
     3361 tgactgcttg tggcgggagc agagactatt caccgccagc cgatgcatct ggagaacaaa
     3421 ttttcagtac ggcctgcacg gaatgccata aacccctcag cgcaaacgtt gccatgatat
     3481 taagcgagaa agtcgctaat aaggatgcaa tcatcaagaa atttcaaggc ggaagcatga
     3541 gaatgccggc attcaaaaat atccagggag aatctgccga tcgattggct gaatacattc
     3601 tagctaatag cgaagtcaaa taatacgaaa ccctgaaaat aaacaacccc gtggcaagac
     3661 cacggggaat cgcaaattaa aaccaaaaaa aacaaaggat cagactcgat agattccgat
     3721 cgtttgtgag aattgcgttt tgctttctct cgatagagca attataaaaa ctaaaagtat
     3781 ggtgctaatt ccagaaaaaa ctatcgagcc tgcccccctt tcttgctttt gtttcttgta
     3841 tgttgtactt gagctggaat agctctagtg attgattgat agaatcagcc gtttctcaaa
     3901 aatgtgttgt atctcaagac ttgattctac ttgcgattct cccaataatt cggatttcga
     3961 tgcaagttca tgactgccag aatgtacaag ccattaggct caattgaata cagaacgcca
     4021 tatggaaatc gcctgacgag gcatcgacgg atgtcacctt gcaaaatcgg ccatgcttcc
     4081 ggataaataa gcgagcgttg tatcgtgtta taaatctcga tagcaaa
//
LOCUS       DAOQLJ010000181         4075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_10937_length_4075_cov_6.576866, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000181 DAOQLJ010000000
VERSION     DAOQLJ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10937_length_4075_cov_6.576866"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PK522_10355"
     CDS             <1..590
                     /locus_tag="PK522_10355"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNC42174.1"
                     /translation="SEGTEVLSDISPQFFIQQLSQRWLARHGADEQAPLSQVEMVPKH
                     TLFFISYCRAPDLPAAIQLVAALKTLGVAEHEIWFDKSAIEPGQDFRERILDGIRTCR
                     YFIPLISSAADQLDEKFFRREWNEAIDRSKSIQGRIFIVPIIADQNYDPEGYIRVPLA
                     WKDNFHFGHAPLGQPNEQTNALLKKLIRSERQQSG"
     gene            594..>4075
                     /locus_tag="PK522_10360"
     CDS             594..>4075
                     /locus_tag="PK522_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42175.1"
                     /translation="MTVEQVSSSLQSMNTQIPVALSPQSPWPGLAAYEEEAHDYFYGR
                     KEEAAELLRMIRLSGLTMLYGKSGLGKTSLLQAGLYPLLRAEHFFPIHLRIDFRPSAP
                     YPPLEQVLQKLLGALTGANADFPTPNENEGLWRYLHRRDLEIWSSDNYLLKPVLVFDQ
                     FEEIFSQGEHYPEQTQLTLNALADLIENRIPQELTIGDKHRQTLSALDLQAQRYRIVL
                     SFREDFLPEIENWKEQAPSLLRNRLRLLPMFRERAIEAVKAAGSEVLAEGVAEPLVDF
                     VGNLEASKLKSLPIIEPVLLSLCCYQLNQRRLPNHKIDIELLQQAGQDILQDFYHEAL
                     AGMPETVSEFIETHLIQGSRYRSSYPVDQALQDKFLTEEQLTRLTSQHRLLRIDQQLG
                     INRIELIHDRLVGVVSQVRDERLKKLEIEKIRAKEKERQRQLALSTALEKQAAAEDLA
                     AERLKATKRAQADAIRLRKQARQLQWALLSVLVLAILAGYGFYNTQTEKKERKLAQRE
                     SISLRLVSESLDITNGLRPGGDERALLQLLAAKQITPNHRKVDAALIDLMNKRVMTDK
                     IWNIGAAWFAITQVQDKTYVLALSNEGKCYFWDPSETWPSEPFLTLSDPNIAALAISN
                     DSLQFAIADHNGEIQVWHRSTKSRVKTFKSLQSVFSIGFSPNGQQLAIGSKGVIQLWD
                     LASGMMIHEMANSQDGAIRAIAFSSDGLEIVSGGEDRRLTVWNSQTGRAVGEPIRGHS
                     NTILSVAFSADGTQIVSSGEDKLLRLWDRQTGKQRIDSLRGHEGIITSVAFIENAQQI
                     VSGSRDGTIRLWSTRDHPIIDTPLLGHEDMVTSVAYSPDGKKIVSGSDDKTLRLWNAW
                     TAQPLDEPLQGHEGTINGVVYSPDGARIVSGASDGTLRFWDAQTGLPIGEPLKGHLEG
                     VETVAYSPDGKLVASGSKDKTIRLWNAYTGKQEGEPLSGHEEMVRSVAFSPDGKVIVS
                     GSYDHTLQRWEVPTGNKLGSPMEGHTEHVVSVAVSPDGQYIASGSKDKTIRLWDAQTG
                     ESINEPIQGHEARINSVAFSPDSQRIISGSHDMTLRLWDVKTGLPVGDPLSGHLGAVT
                     SAVFSPDGSHILSGSADKTLRLWMANTDWVDELCNKLTRNMSHAEWQEWVSPNIKYRK
                     QCLNLPIYESNTLIG"
BASE COUNT         1149 a          824 c         1009 g         1093 t
ORIGIN      
        1 atagtgaagg aaccgaagtg ctatccgaca tctctccgca attttttatc cagcaacttt
       61 ctcagcgctg gttagcgcgt catggtgcag atgagcaagc gccattatcc caagttgaaa
      121 tggttcccaa acatacactc tttttcatta gctattgccg tgcgccggat ttgcctgcag
      181 caatacaact ggtagcggca ctgaaaacat tgggtgttgc agaacatgaa atatggttcg
      241 ataaatcggc gatcgaaccg ggtcaagatt tccgggagcg aattctagac ggtatccgta
      301 cttgccgcta ttttattccg ctaatttcca gcgctgctga tcaattagat gagaaattct
      361 tccggcgtga atggaatgaa gcaatcgatc gaagcaaatc aattcaaggg cgtatcttta
      421 tcgttcccat tatcgctgat cagaattacg atccagaagg ttacattcgg gttccattgg
      481 cctggaagga taattttcat ttcggccatg caccactggg tcaacccaat gagcaaacca
      541 atgcgctact caaaaaatta attcgatcgg aacgacaaca gagtggctag gttatgactg
      601 tagagcaagt ttcatcatct ttacaatcga tgaataccca gataccagtg gctttgtcac
      661 cccaatcgcc atggccagga ttggcagcgt atgaggaaga ggcgcacgat tatttttacg
      721 ggcgcaagga ggaagcagcc gagttattgc gcatgattcg cttatctgga ttgaccatgc
      781 tttatggtaa gtcaggttta ggaaaaacgt cattgttaca agctggactt tatccactgt
      841 tacgtgcaga acattttttc ccgattcatt tacgcatcga tttcagacct tctgcacctt
      901 atccacctct agagcaagta ttgcagaagt tattaggtgc attaacgggg gcaaacgccg
      961 attttccaac tccaaatgaa aacgagggat tatggcgtta tctccatcgg cgagatcttg
     1021 aaatttggag tagcgataat tatttgctaa aacctgttct cgtttttgat caatttgaag
     1081 aaattttttc gcaaggtgag cattaccccg aacaaacaca attaacactc aacgcattag
     1141 ctgacctgat tgagaatcga attccacaag aattgacaat aggcgataag catcggcaaa
     1201 ccctatcagc attggattta caggcacaac gctatcgaat cgtactttca tttcgtgaag
     1261 attttcttcc tgagattgag aattggaagg agcaagcgcc ttcattgctc cgtaatcgct
     1321 tacgtttatt accgatgttt cgggaacgtg ccattgaagc ggttaaagct gccggctctg
     1381 aggtgctagc cgaaggcgta gctgaaccgc ttgttgattt tgtagggaat ctcgaggcat
     1441 ctaagctgaa atcattaccg attattgaac ccgttttact aagtttgtgt tgttatcagc
     1501 tcaatcagcg acgtttacca aaccataaaa tcgatattga gttattacaa caagcggggc
     1561 aggatattct gcaggatttt taccacgaag cgctggcagg tatgccagaa acagtatccg
     1621 aattcattga aacccatttg atacaaggga gccggtatcg aagtagctat ccagttgatc
     1681 aagcgttaca agataaattt ttaacagaag aacaattgac tcgactgact agtcaacatc
     1741 gtttactacg cattgatcaa caattaggga ttaatcgcat agaactcatt catgatcgat
     1801 tagtgggtgt tgtgagccag gtaagggatg agcgtttaaa gaaacttgag atcgagaaga
     1861 ttcgcgcaaa agaaaaagaa cggcaacgcc aactggcgtt atctactgcg cttgagaaac
     1921 aagctgctgc agaagatttg gctgcagagc ggttaaaagc aaccaaacgt gcgcaggcgg
     1981 atgcaattcg tcttcggaaa caagcaagac aattacaatg ggctttgttg agtgttttag
     2041 tactggctat tttggctggc tatggttttt ataacacgca gactgagaaa aaagaacgaa
     2101 aactggcgca acgagaatca atcagccttc ggttggtatc ggagagcctt gatattacca
     2161 atggactgcg tccgggtggc gatgagcggg cgctgttgca actgttagca gcaaagcaga
     2221 ttacgccgaa tcatcgcaag gtcgatgcag cactgattga tctcatgaat aaaagagtaa
     2281 tgacggataa gatatggaat attggtgcgg catggtttgc aatcacccag gtgcaggata
     2341 aaacctatgt tcttgcactg agcaatgaag gtaagtgtta tttctgggat ccctcagaaa
     2401 catggccgag tgaacccttt ttgacattaa gtgatcccaa tatcgcggca ttggctatct
     2461 ccaatgatag tcttcaattt gcgatcgctg accataatgg ggagattcaa gtttggcatc
     2521 gatccactaa atcccgagta aaaactttta aaagcctgca atctgttttt agcatcggct
     2581 tttctcctaa tggccagcaa ctcgcaatcg gtagtaaagg ggtgattcaa ttgtgggatt
     2641 tggcatcagg aatgatgatc catgaaatgg ctaactcgca agatggggcg attcgcgcga
     2701 tcgctttctc aagtgatgga cttgaaatcg tttccggggg agaagatagg cgcttaaccg
     2761 tttggaatag ccaaactggt cgtgcggttg gtgagccgat tcgagggcac tcaaacacta
     2821 ttcttagtgt tgcgttttct gctgatggaa ctcagattgt atcgagtggt gaagataaac
     2881 tgctgcgatt atgggatcgc cagactggta aacagcgaat cgactcctta cgtggccatg
     2941 aaggaataat taccagtgtt gctttcattg aaaatgcaca acaaatcgtt tctggtagtc
     3001 gagatggcac cattcgttta tggtcgactc gtgatcaccc catcatcgat acccctttat
     3061 tgggacatga agatatggtt acgagtgtag cctatagccc agatggtaag aagattgtgt
     3121 cgggtagcga tgataaaaca ctgcgattat ggaatgcttg gacagcacaa ccgcttgatg
     3181 aaccactaca aggccatgaa ggaacaatca atggtgtggt ctatagtccc gatggggcac
     3241 gtattgtttc tggtgccagt gatggtacgt tacgattctg ggatgcgcaa actgggttac
     3301 cgattggtga acccttaaaa ggccatctcg aaggtgtaga aacagttgcg tatagtccgg
     3361 atggtaaact cgttgcatct gggagcaaag ataaaaccat ccgattatgg aatgcttata
     3421 caggaaaaca ggaaggggaa ccgttatccg ggcatgaaga aatggtacgt agcgtagctt
     3481 ttagtcccga tggaaaagtc attgtatccg gtagctatga tcatacctta cagcgctggg
     3541 aagtcccgac aggcaataaa ttgggtagtc cgatggaagg ccatactgag cacgttgtta
     3601 gtgtggcagt tagtcccgat ggtcaatata ttgcttcggg tagcaaagac aaaacgattc
     3661 ggttatggga tgcacagacg ggtgagtcga ttaatgaacc gattcaaggc catgaagcac
     3721 gtatcaatag tgttgctttt agccccgata gtcaacgtat tatttctggc agtcacgata
     3781 tgacgttgcg cctttgggat gtgaaaacgg gtttgccggt tggtgatccc ttatcaggac
     3841 atcttggtgc ggttacgagc gctgtgttta gcccagatgg ttcccacatt ttatcgggaa
     3901 gtgcggacaa aacgttgcgc ctgtggatgg ctaacacgga ttgggtagat gagctttgta
     3961 ataaattgac gcgtaatatg agtcatgcgg agtggcaaga gtgggtttca ccgaatatta
     4021 agtatcggaa acaatgtctc aatttaccga tttatgaatc caataccttg attgg
//
LOCUS       DAOQLJ010000182         4073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_10947_length_4073_cov_5.236187, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000182 DAOQLJ010000000
VERSION     DAOQLJ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10947_length_4073_cov_5.236187"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PK522_10365"
     CDS             complement(<1..607)
                     /locus_tag="PK522_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNC42176.1"
                     /translation="MSTSIYGKRMRKISITFILMALWLLSSVAWAATHTISISAVQLP
                     NGQYGYKLNNHTSSDGAVPNYPAEAVIPGPTLFVKQGDQVTVQLRNETNIKIGFNVPG
                     LTNGNVAPANPGQTKSYKLNTQRTGTYPYFDEKSQLLGLFGAIVVDDASGQVQSFVDG
                     DGKITTVNRSQLDKEFVMFMVGSTFWGAEISRDGTQKPLWTN"
     gene            complement(627..1652)
                     /locus_tag="PK522_10370"
     CDS             complement(627..1652)
                     /locus_tag="PK522_10370"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNC42177.1"
                     /translation="MKDKLEYEFQSRSCHALPKPKELANPKLASWLVLASMVLGLGGV
                     TSVLAGNTHQIKMVAEEVDPTGNLGLYAYRMMQHQVVDGNGNTVDLSGNYSAVATIPG
                     PTVIMSEGDVAEIELMHQFDPDNPKQEHVSLHVHGVHYDKESDGTLKYINLYKDESAT
                     PVLSYNYRWVAAPGTAGTWAYHDHNMNSHNGAEDRGLFGALIVNPASGFLPQDINGTG
                     SGNTPLSRIQKEFVLYIGDDAFWGMEINNANGAQTALGVNPNLNAQRNSDIRIHLIAL
                     GTNFHQFELPSYTWVDPGTRRRISAKAIGPLEKHVFAISASHSASYLDTAFSSKLLGM
                     KGQLIVD"
     gene            2471..2863
                     /locus_tag="PK522_10375"
     CDS             2471..2863
                     /locus_tag="PK522_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42178.1"
                     /translation="MNFETEMRARFGGFWNSLLLLLIIIIFLRFSAIELIAEGLGAKL
                     SLSLKLFFGFALFGVVLALFKMLFSSSNPPYYRLPASVNIAWGLKRGLVIGGILMLII
                     GILHHDNFLGVLQPLLEKLVGKIIGIFG"
     gene            complement(2936..3379)
                     /locus_tag="PK522_10380"
     CDS             complement(2936..3379)
                     /locus_tag="PK522_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42179.1"
                     /translation="MDELTIHLKSSQQLTLMFSVVHVACIVIIALLDGSILLKASGML
                     LLVLSLIYYIRLYALLQSPNAIVSFYLSEHSTCITQTRSGQQLHWHIQGSSFVTPYLT
                     VLSLKSNQSFFDRSIVIFPDSINAEQFRQLRVWLRWKWKDTNSLE"
     gene            3405..>4073
                     /locus_tag="PK522_10385"
     CDS             3405..>4073
                     /locus_tag="PK522_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HNC42180.1"
                     /translation="MNELNQSINENLLDLQKIPIEKGVVDLSDFGIIRFTGEDTLTFL
                     QGQLSCDVLKVTPTQAQHGCYCSPKGRILANFILAADHTGEAWYMQMPRAILDKIVKR
                     LSMYVLRAKVKLVDYSAESIRLGIVGSQAHSLLEKSLPTLTMPESRLSVAQVAGITVI
                     CLGPNRFEMIAPFEQGNQLKTYFDKATSWVNLSYWQWLDIQAGIPTISTETQEQFIPQ
                     MVNMD"
BASE COUNT         1183 a          855 c          826 g         1209 t
ORIGIN      
        1 ggttcgtcca taatggtttt tgtgtaccat cacggctgat ctcggctccc cagaaggtag
       61 agcccaccat gaacatgaca aattccttgt ccagttgaga gcgattaacc gtagtaattt
      121 tgccatcacc atcgacgaaa ctctgcactt gaccactggc atcatcgacg acaatcgcac
      181 caaacaagcc taaaagttgc gatttctcgt caaaataggg gtaggttcct gtacgttgcg
      241 tatttaattt atagcttttg gtttgtccag ggtttgcagg cgcaacattg ccattcgtta
      301 atcctggtac attgaaccca attttaatat tggtctcatt acgcagttga accgttactt
      361 gatccccttg cttcacgaat aaagtagggc caggaatcac ggcttctgct gggtaattgg
      421 gtaccgctcc atcgctactg gtgtgattat tcaatttata gccatactgg ccattcggta
      481 attgcaccgc tgaaattgaa atcgtatggg ttgctgccca tgcaaccgat gatagcaacc
      541 atagtgccat caatataaac gttattgaaa tctttctcat tctttttcca taaatagatg
      601 tactcatgac cttctcccaa taaatcttag tcaacaatca attgaccctt cattcccagt
      661 aatttgctgg agaaggcggt gtctaagtaa ctcgctgaat gtgaagcact aattgcaaac
      721 acatgctttt ctagcggccc aatggcttta gcactaattc ttctgcgagt gccgggatca
      781 acccaggtat agctcggtag ctcaaactga tgaaagttag tgcctaacgc aatcaaatga
      841 atccgaatat cagaatttct ttgtgcgttg agattgggat taacgcccag tgccgtttgc
      901 gcaccattgg cattattaat ttccatcccc cagaaagcat cgtctccgat gtacagtaca
      961 aattctttct gaatccgact caacggggta ttgccgctgc cagtgccatt aatatcctgt
     1021 ggcaagaatc ctgaagcagg gttgacgatc aacgcaccaa atagcccacg atcttcagca
     1081 ccattatgac tattcatatt gtgatcatga taagcccaag ttcctgcagt gcctggcgcg
     1141 gctacccagc ggtagttata agacaatacc ggtgttgcac tttcatcttt atagaggttg
     1201 atatatttga gtgtgccgtc actttctttg tcgtagtgca caccatgaac atgcaaactc
     1261 acatgctctt gtttaggatt atccggatca aactgatgca tcaattcgat ttctgccaca
     1321 tcgccttccg acataatgac cgtaggccca gggatagtcg ccacagcgct ataattgccg
     1381 gaaaggtcaa ccgtattacc attaccatcg acaacttggt gctgcatcat ccggtaagca
     1441 tataatccta aatttccggt agggtctact tcttccgcaa ccattttgat ctgatgggtg
     1501 ttgcctgcca gaactgaagt cactcctccc aatcctaata ccatggaagc caaaacaagc
     1561 caggatgcta attttgggtt ggccaactct ttcggcttag gtagtgcatg acatgagcga
     1621 gactgaaact catattcaag tttgtctttc attaaattcc ctccattttg attcaaccaa
     1681 aaaaaagcct gtctgaccca attctaatgg gccatgacaa gctgctttgc tcctacttca
     1741 atttctgtga atacactctt attctttgga aaacacttct caaaataatg taaccattca
     1801 caaaaattta ataattatta ttgttatgag agttaaacga gcataaatta tgccaataat
     1861 aatttctatt attattcaat aactagcgtt gatattctta tctgaaatga ttaagcggat
     1921 gtaatatcag ctctttctgt aaaaaaaacc gacgctttgg gggtcaaaaa tgctcgtaat
     1981 atgaacatct agcatttgtc aatatgtcca ggtgcgacaa attgtcgcac ctgagcttaa
     2041 caattaatta acaaaagtga catgtaaaat tttctgacaa ttgtgggtct gaatatagat
     2101 taaattgacg gttacagccg ctaaaaatcg ccgcgcattt cttgaagatt taatccattg
     2161 aagttaagca gtatcaatta atgctttatt ttcaattgac tgatgaaaaa tcttggggtg
     2221 ttcagagaag tctatcaaaa atatcgacat cactatgctg gctgataaag cctcttacgt
     2281 aaaataaagc gatgttctct accatatccc aatggagaac gattaactta acaacgctag
     2341 caatttttaa gttgtaaaag caattttact ttagaccgtg ataccatcat tccttcagaa
     2401 aattgaatct gaaaaaaatt aaaaaacagt taaatttaag gccatttcaa tttttcacgg
     2461 gagggaatag atgaacttcg aaacggagat gagagcgcga tttgggggct tttggaattc
     2521 gctattgctg ttgctcatta tcattatttt tttaagattc tctgcaattg agttaatagc
     2581 agaaggactg ggtgcaaagt tatcactatc actcaaatta ttttttgggt ttgcattatt
     2641 tggggtagtg ctcgccttgt tcaaaatgtt gttttcttct tccaatcccc cttattatcg
     2701 tctgcccgct tcagtcaata ttgcatgggg actcaaacgg ggtttagtta tcggtggtat
     2761 tttaatgctg attattggca ttttgcatca tgacaatttt ttaggggtat tgcagccatt
     2821 gcttgaaaag ctcgtgggta aaatcatcgg tatatttggt tagtttggtt gtatcatcat
     2881 ttaaacgttg cacgcgcttg ttatgagtta gtgtgcaacg ttatggtgtg ttggatcatt
     2941 ctagggaatt agtatctttc catttccatc ggagccagac tctgagttgg cggaactgtt
     3001 cggcgttgat actatctgga aaaatgacaa tactgcgatc aaaaaacgat tgattcgatt
     3061 tcaaactgag cacggtcaga taaggagtca caaagctact gccctggata tgccagtgta
     3121 gctgctgccc tgatcgcgtt tgggtgatac aagtggaatg ctccgataga taaaacgaca
     3181 cgatggcatt gggtgattga agcaatgcat atagccgaat atagtaaatc aagctcagca
     3241 caagcaatag cattccactt gccttcaaca gtatcgaacc atccagcagc gcaatgataa
     3301 cgatacaagc cacatggacg accgaaaaca ttagagtcaa ctgctgggaa gatttgaggt
     3361 gtatcgttaa ctcatccatc gcattaaatt aaaaaattgc agtaatgaat gaactgaatc
     3421 aatctattaa tgaaaatctg ctcgatctac aaaaaatacc gatagagaaa ggggttgttg
     3481 atctttcaga ctttgggatc atccgcttta ctggcgaaga tactttaaca tttctgcaag
     3541 gacaattgag ctgtgatgtt ctgaaagtta cgccaactca agctcagcat ggttgttact
     3601 gttcaccgaa gggacgcatt cttgccaatt ttatccttgc ggctgatcac acaggagaag
     3661 cttggtacat gcaaatgcca cgcgccattc tagacaaaat cgttaaacgg ctatccatgt
     3721 atgtgctgcg ggccaaagta aaattagtgg actatagcgc ggaatcaatc cggctgggaa
     3781 ttgtaggtag tcaagcgcat tctttgcttg aaaaaagttt gccaacatta acaatgccgg
     3841 agtctcgttt aagcgtcgca caagtagcgg gtataaccgt aatctgcctg ggacccaatc
     3901 gctttgaaat gattgcacct tttgagcaag gcaatcagct caaaacgtat tttgataaag
     3961 cgacatcctg ggtaaatcta tcctattggc agtggctcga tatccaagcg ggtattccga
     4021 caatctcgac tgaaactcag gaacaattta taccacaaat ggtaaacatg gat
//
LOCUS       DAOQLJ010000183         4058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_11011_length_4058_cov_7.018236, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000183 DAOQLJ010000000
VERSION     DAOQLJ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11011_length_4058_cov_7.018236"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PK522_10390"
     CDS             <1..332
                     /locus_tag="PK522_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HNC42181.1"
                     /translation="KGLIDWETSLSIPLYLRMFDPQHANLAFIGLLQPQGCLWKLTEV
                     QAKLLSLLLTQQIQLPENWRSLALTEGSDWSKQFLTRPRHSLEVHYPAYLSQLQRIIT
                     GKILHDQ"
     gene            complement(357..1358)
                     /locus_tag="PK522_10395"
     CDS             complement(357..1358)
                     /locus_tag="PK522_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNC42182.1"
                     /translation="MKNDCSFSQQEVLTTIREFEKVLILYNPISSAGNTAELASDFED
                     FLIKQRFSVSVYETAKKMKSYQRIVADIAASNLIVVIGGDGTVRKLLPLLSQTHTPAY
                     VVPGGNESLIARSYGMTTQCTDLLQAMTTGKYLEQYYGLISGEGIQGKKPFFIMASMG
                     FDSLTIKRIGKRKGPINDAAYVWCGLNALVSLHHPVISVIVDGNTVIDQQSGYLIVAN
                     SAAYARNLQLVPEANPSNQELVLGFLPKAKHQHEWIKLTRMLQHKPAGLPLQYFSGKQ
                     ITLTLHHPSYPLQVDGDYFRNRDIEEGATIEFSIHPEPIRVLVPPDYCIQSTAIKSA"
     gene            1633..1977
                     /locus_tag="PK522_10400"
     CDS             1633..1977
                     /locus_tag="PK522_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HNC42183.1"
                     /translation="MKKIIHISLFVTIFFLFSNQAAANDDYFQDSADKFANGIINATT
                     GWIELPKNIVLFSQKEGPLYGLTLGLGMGVMHTVGRSLVGIANAATFFIPTKPSISPL
                     YVWDDFSKETTY"
     gene            complement(1971..>4058)
                     /locus_tag="PK522_10405"
     CDS             complement(1971..>4058)
                     /locus_tag="PK522_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNC42184.1"
                     /translation="TSDQTEIGRISRLLEQVDMLETPLLRQMASFGRWLSMVIGVLTV
                     CIFAYGLWIGRYSTHEMFLAAVGIAVAAIPEGLPAIMTITLALGVQRMAKQHAIIRRL
                     PAVEALGSVTVICTDKTGTLTRNEMTVQRIITTHHQLEVSGVGYQPQGGFSENNQAVC
                     LDTYQGEHRELYDLIRTGLLCNDAQLRQQGDDWCVEGDPTEGALITLALKADLKPILE
                     HESLPRIDTIPFESQHRFMATLHHTHTGVAMVLVKGAPETILAMCSREGEWHRNRPLI
                     AHYWQQAVQASAMQGQRVLALAAKSVDIQQTTLNAEQMLGGFTILGLVGIIDPPREEA
                     IRAVSLCQSAGIRVKMITGDHATTAQAIGVQLGIGDGQTSLSGQEIEYMDAIALQSTV
                     LENDVFARASPEHKLRLVEALQARGEIVAMTGDGVNDAPALKRADIGVAMGKKGTEVA
                     KEAAEMVITDDNFASITRAIEEGRTVYDNIRKTIMYIMPTSGGEAGMLIIAILLGMTL
                     PISPVQILWVNMVTAITLSLSLAFEKAEPGIMQHLPRDPSKSILSGYMIWRIVLVSTF
                     LTIGGLALFLSGQAQGMSLEACRTLAVNALVMGEICYLFNCRYLSASALSYQGIFGNR
                     YALLAIGLLAILQLLFTYHPLMQQWFGTAAIDFIAWFKILALGVGLFLIIEFEKWVMR
                     RHRAASPLYSKHQ"
BASE COUNT         1138 a          975 c          807 g         1138 t
ORIGIN      
        1 caaagggttt aatagattgg gaaacgtcgc tctccattcc tctttactta agaatgtttg
       61 atcctcagca tgccaattta gcctttattg gtttgctgca accgcaaggc tgtctttgga
      121 aactaaccga agtgcaagcg aaattactca gcctattatt aacacaacaa atccaacttc
      181 ccgaaaattg gcgctcgctc gctttaactg aaggttctga ttggtcaaag caatttttaa
      241 cccgaccaag gcattcactg gaagtacatt accccgctta tcttagtcaa ttgcagcgca
      301 tcattactgg aaaaatcctt catgatcaat agtgataagg aatcaaccct ttgcgactag
      361 gcgctcttga tagccgttga ttggatgcaa taatccgggg gcacgagaac ccgaattggt
      421 tctgggtgaa tactgaattc aatggtagca ccttcctcaa tatcacgatt gcggaaataa
      481 tcaccatcaa cctgtaaggg ataagaagga tggtgaagtg ttaaggttat ctgtttgccc
      541 gaaaaatatt gcaggggtaa tccagctggt ttatgttgca acatacgagt cagcttaatc
      601 cattcatgct gatgtttggc tttcggtaga aagcccagaa caagctcctg attggatgga
      661 tttgcttcgg gcacaagctg taaatttctc gcataggcag cgctgtttgc aacaattaaa
      721 tatcctgact gctgatcaat cacggtattt ccatcgacga taaccgaaat cactgggtga
      781 tgcagtgata ctagtgcatt taaaccgcac caaacatagg ccgcatcatt gattggccct
      841 ttacgtttac caatgcgctt gattgtaagc gaatcaaacc ccatcgatgc catgataaaa
      901 aaaggttttt ttccttgaat accttcacct gaaatcaatc catagtactg ctccagatat
      961 ttccctgtcg tcatggcttg taataaatca gtacattgtg tagtcattcc ataagatcgt
     1021 gcaatcagtg attcatttcc gcctggcacc acataagctg gcgtatgggt ttgactcaat
     1081 aaaggtagta gcttcctgac cgttccatca ccgccaatca cgacaattaa attactagct
     1141 gcaatatctg ccacaatgcg ctgatacgat ttcattttct tggccgtttc atagaccgat
     1201 acggaaaagc gctgtttgat gagaaaatct tcgaaatcgc ttgctaattc agcagtgttg
     1261 cccgcactgg aaataggatt gtaaagaatc aatacttttt caaattcacg gatagtagtc
     1321 aaaacttcct gctgcgaaaa tgagcaatcg tttttcaatg aaattgcctt taaatgtaaa
     1381 ccgagtagtg gaatgaattc tggacaaata cgagtttaag taagcgtatc ctagattttt
     1441 tacaaaatca tgtcacaacc ctattgattc acttcaagct aaaaactctg atcaatcaga
     1501 aaaagtgata gcagaatttt tgtaatgctt attcattatt atgaatttat aaatgtaaat
     1561 gcttacgcta atatataagg cacgcatcag aaacttatcc aatttaaact tagctgaact
     1621 caggaattgc ccatgaaaaa aataattcat atttctttat ttgttacgat ctttttcctt
     1681 ttctctaacc aagcggcagc gaatgacgac tatttccaag attcggctga taaatttgca
     1741 aatgggatca ttaatgcaac cacgggttgg atcgaattac ctaagaatat cgttttattc
     1801 agccaaaaag agggtccact ttatggctta acactcggtc tcggtatggg tgtcatgcac
     1861 acggttggtc gcagtttagt gggtattgcc aatgcggcta ctttcttcat tcccacaaaa
     1921 ccatcgataa gtcccttgta tgtctgggac gatttttcaa aagaaacaac ttattgatgc
     1981 tttgaatata gcggcgaagc tgcccggtgc cgcctcatca cccatttctc aaactcaatg
     2041 atcaagaaaa gaccaacacc tagcgcgagt atcttaaacc aggcaataaa atcgatagct
     2101 gccgttccaa accattgttg catcaaggga tggtaggtaa atagcaattg aagaatcgcc
     2161 aataggccaa ttgccaatag cgcataacgg ttgccgaaaa tgccttggta agataatgca
     2221 gaagcagata aataacggca attgaataag taacaaattt cacccattac taatgcgttt
     2281 accgcgaggg tacggcaagc ttctaggctc atcccttgag cttgccccga tagaaataaa
     2341 gccaaccctc cgatcgtaag gaacgtcgaa actaaaacaa ttcgccaaat catataccca
     2401 gatagaatcg atttacttgg atcacgcggt aaatgctgca tgatgcctgg ttctgctttc
     2461 tcaaacgcga gcgataaaga aagggtaatc gccgtcacca tgttaaccca taggatctgt
     2521 accggagaaa tgggtaaagt catgccaaga aggatagcaa taatcagcat acctgcttct
     2581 cccccactgg ttggcataat gtacataatc gttttacgaa tattatcgta aactgtgcgg
     2641 ccttcttcga ttgcgcgcgt aatcgaagcg aaattatcat cggtgataac catttctgct
     2701 gcttccttgg ccacttccgt ccctttcttt cccatggcta caccaatatc cgctcgtttc
     2761 aatgcaggcg catcattgac accatcacct gtcatggcca cgatttcgcc tctcgcttgt
     2821 aacgcctcga ccaagcgcaa tttatgttct ggactcgcac gggcaaatac atcgttttcc
     2881 aatactgtag attgcagcgc tatcgcatcc atgtattcga tttcctgacc acttaacgat
     2941 gtttgtccat caccaatgcc tagctgtacc ccaattgcct gagctgtcgt tgcatgatcg
     3001 cctgtgatca tttttacacg gattccagcc gattggcaca ggcttacggc tctgatggct
     3061 tcttcacgcg gcgggtcgat gattcccact agccccagaa tcgtaaatcc acctaacatt
     3121 tgttcagcgt tcaacgtagt ttgctggata tcgacgcttt tagccgcaag cgccaagaca
     3181 cgctgccctt gcatcgctga tgcttgcact gcttgttgcc agtagtgtgc aatcagcggt
     3241 cgattgcgat gccattcccc ctctctggaa cacattgcca atatcgtttc aggtgctcct
     3301 ttaactaata ccattgctac accagtatgc gtatgatgta acgttgccat gaaacgatgc
     3361 tgcgattcaa acgggatggt atcgattcgc ggtagtgatt catgctccag tatcggttta
     3421 agatccgctt ttagcgccaa cgtgattaat gcaccttccg ttggatcacc ttccacacac
     3481 caatcatcgc cctgttgccg cagctgcgca tcgttacata gcaatcccgt ccgaattaaa
     3541 tcatagagtt ctcgatgttc accttgatag gtatcgagac aaaccgcttg attattctca
     3601 ctaaaaccac cctgcggttg atatcctacc ccactaactt ctaattgatg gtgtgtggta
     3661 ataatgcgtt gcaccgtcat ctcgttacgc gttaacgtac ccgttttatc ggtacaaatc
     3721 acggtaaccg accctaacgc ttcgactgct ggcagacggc gaataatcgc atgttgcttc
     3781 gccattcgtt gtacacctaa cgcaagcgta atggtcatga ttgcaggcag tccttcgggt
     3841 atcgctgcaa ctgcaatacc tacagccgca agaaacattt catgcgtgct gtaccttcct
     3901 atccataaac cgtaagcaaa aatacaaaca gtcaatacgc ctatcaccat actgagccag
     3961 cgcccaaaac tcgccatttg tctgagcagt ggcgtttcta gcatatcaac ttgctccaac
     4021 aagcggctaa tgcgaccgat ttcagtctga tcactggt
//
LOCUS       DAOQLJ010000184         4036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_11099_length_4036_cov_5.425772, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000184 DAOQLJ010000000
VERSION     DAOQLJ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11099_length_4036_cov_5.425772"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            481..900
                     /locus_tag="PK522_10410"
     CDS             481..900
                     /locus_tag="PK522_10410"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNC42185.1"
                     /translation="MFRMIFRRIYVAIDQSEFSGQALIAAMKIAQTSKVLLCPGYCIT
                     DDTLSSQETALNFLTGIKSQISDAVEVEIKIAAADVQYGLNGISTAIAQSAHNWQSDL
                     LVVGTPNREGAKYFYLSAVAGELISKVNCSVMLIRIN"
     gene            1140..1592
                     /locus_tag="PK522_10415"
     CDS             1140..1592
                     /locus_tag="PK522_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC42186.1"
                     /translation="MTQYFTKSAARNIFYGGSVFFLLIFIALTIHSHFYAISTATDES
                     TLTESVIRGKHIWERHSCINCHSLIGEGAYFAPELGNVWIRYGGQQSPEGARTGLKAW
                     MRAQPTRIEGRRQMPQFNLSEQELDDLIDFLEWTSRINTLGWPPSISG"
     gene            1650..2993
                     /locus_tag="PK522_10420"
     CDS             1650..2993
                     /locus_tag="PK522_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNC42187.1"
                     /translation="MKYQTQALAYPYFVAALVLFLVQILAGIMAGSIYVFPNLLSEIT
                     PFHILRMIHTNALLVWLLLGYFGASYFLIPEESEREIHSSRLAWLQLGFFVFAGLAAV
                     ASYLSGVHEGREFLEQPLWIKILMVVAFLIFIYNVTMTVLQGRKTVVSIVLLLGLWGA
                     AIFFMFAFYNPDNLSVDKLYWWWVVHVWVEGVWELIMSAMLAFLLIKMTGVDREIIEK
                     WLYVIVGFSLFSGLLGTGHHYYWIGTPEYWHWIGIVFSFLEVVPFFAMIVWSFYLVRK
                     SGRNHPNKAALFWSLGCPIMAFIGAGLLGLMHSFPSINFYTHGTQLTAAHGHLAFYGA
                     YVMLNLAFFTYAMPILRNSQPFNQNLNILSFWLMNAAMIGITFALLFAGILQTHLQRV
                     MNLGYMQVQSQLELFYWLRLGSGVFFIVGALIFIYSVLGPNREAFVAGKFQHYNV"
     gene            2993..3826
                     /locus_tag="PK522_10425"
     CDS             2993..3826
                     /locus_tag="PK522_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNC42188.1"
                     /translation="MAHDSVFAAAKGPVSAQSATEIPFYHETGNECALFAAAYREGLP
                     LLLKGPTGCGKTRFVAHMAARLGRPLHTVSCHDDLTAADLTGRYLLQGGETTWLDGPL
                     TRAVREGGICYLDEIVEARKDVTVVLHPLTDDRRILPLERTGEFLQAPDDFMLVVSYN
                     PGYQNILKSLKPSTRQRFVTINFDFPPPIIETRIVATESGLAEERCRLLVDLAQRIRV
                     LKNIDLEEVVSTRLLIYCAILLKNGFDPYQAAQATLIEPLCEDDDVKQGLLELVRATF
                     G"
     gene            3830..>4036
                     /locus_tag="PK522_10430"
     CDS             3830..>4036
                     /locus_tag="PK522_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein norD"
                     /protein_id="HNC42189.1"
                     /translation="MNWLNFTELEEQAGRFWHRIVGSAESYPNYQQAAITLDQVRPAL
                     GIFFRGLGGASGVAIQSGSPRSSGH"
BASE COUNT         1065 a          788 c          898 g         1285 t
ORIGIN      
        1 agtatcggaa acaatgtctc aatttaccga tttatgaatc caataccttg attggggaat
       61 cggtcaaaat gctttctgaa aaaggggggt agaacacgcc aaatcttgcg tttcttgtaa
      121 ttcgagtttt tgctagtaat cttcatcaga taattaaaaa cgatagtatt aatgggaaaa
      181 ttattattac cgactatctg catatacttc ttctcaacta tatctaatga aaagtattac
      241 ctctttctaa atatattcat tatataaaat taatactttg cccaatatac ggaacacttt
      301 ctatagcagt agactattct cgtaattcaa gtttaagcat gtttttctga attgctagct
      361 tgtatttatt taactggagc aacaaaagag actagttcat ggcagcaaag ataagtaaaa
      421 gtactattgg ttacgcgcca catcattctg aacataatta actattagaa ataagtaatt
      481 atgttcagaa tgatattcag aagaatctat gttgcgattg atcaaagtga attttcgggg
      541 caagcattaa tagctgctat gaaaattgct caaacttcca aagtgttgct atgcccgggg
      601 tattgcatca ctgatgacac gctatctagc caagaaacag ccctcaattt cttaacaggc
      661 attaaatctc aaatcagtga tgcggtcgag gttgaaataa aaattgcggc tgctgatgtg
      721 caatatggtt tgaacggaat cagtactgct attgctcaat ctgcacataa ttggcaatcg
      781 gatttattag tggttggtac tccaaatcga gaaggagcta aatatttcta tctgagcgct
      841 gtagcaggag agttaattag taaagtcaat tgttcagtaa tgctgataag aatcaattga
      901 ttctcaaata ctttattaaa tgtgaagtaa agcagtatga gtagctcctc tgataccgct
      961 taatttttga gagcctagct gtattgaatc aaattatttc aacaaattaa tttgtcctcc
     1021 ataaatttaa attataatcg attggcatct tgaaaaaata tacataacca tatgattatt
     1081 attgttaaat tgatgtttta ttagaataat attggctatt aattaaggaa caaatctata
     1141 tgacacagta tttcactaaa tctgctgcac gaaatatctt ttacggcgga tcagtttttt
     1201 ttctactgat atttatagcg ttgacgattc atagccactt ttatgcgatc agtactgcca
     1261 cggatgaatc aacattaacg gaatcggtca ttcgtggaaa gcatatctgg gagcgtcatt
     1321 cctgtatcaa ctgtcattca ctaatcggtg aaggggcata tttcgcacct gaacttggga
     1381 atgtttggat tcgctacggt gggcagcaaa gtcctgaagg cgcaaggacc gggctcaaag
     1441 catggatgcg tgcacaacct actcgaatcg aaggtcgacg gcagatgccc cagtttaatt
     1501 tgtcagaaca ggagctcgac gatcttatcg attttctgga atggactagt cgcatcaata
     1561 cacttggttg gccccctagc atttctggat gattccattg ggaccgttta tatgcattca
     1621 tatttttgta gcataggaga cacatatcca tgaagtatca aactcaagca ctggcttatc
     1681 catattttgt cgctgcactg gtattatttc tagtgcaaat tctggcagga attatggctg
     1741 gctcaattta cgtatttcca aaccttctat ccgagatcac gccatttcat atcctgcgca
     1801 tgatccatac caatgcatta ttagtttggt tgttgttggg ttattttggt gcgagttatt
     1861 tcctaatacc ggaagagagt gagcgggaaa ttcacagttc cagattggcc tggctacagc
     1921 ttgggttttt tgtttttgct ggactcgctg ctgtcgctag ttatttatca ggagtgcacg
     1981 aaggccggga atttttagag caaccattgt ggatcaagat tctgatggtt gttgcatttc
     2041 taatttttat ctataacgtt accatgacag tgctgcaagg acgcaagact gtggtatcga
     2101 ttgttttgtt gctaggattg tggggagcag ccattttttt catgtttgca ttttataacc
     2161 cggacaatct ttcagtggat aaactttatt ggtggtgggt agtgcatgtg tgggtcgaag
     2221 gtgtctggga gctcatcatg tcggctatgc tggcatttct attgatcaaa atgacggggg
     2281 tcgatcgtga aattattgaa aagtggttgt atgtcatcgt tggcttttct ttatttagtg
     2341 gattgctagg aactggacat cactattatt ggattggtac gccggaatac tggcattgga
     2401 tcggcattgt tttctctttc ctcgaagtcg ttccattttt tgccatgatt gtgtggtctt
     2461 tttatctggt tagaaaaagc ggccgtaatc accccaataa agctgcttta ttttggagtt
     2521 tgggctgtcc gatcatggcg tttattggtg ccggattact gggattgatg catagttttc
     2581 catcgatcaa tttctatacg cacggcacac aactaacagc agcacatgga catttggctt
     2641 tttatggtgc ctatgtgatg ctgaatttgg ctttctttac ctatgccatg cctattctcc
     2701 gtaattcgca gccgttcaat cagaatttga acatcttaag cttctggctc atgaatgctg
     2761 ccatgattgg cattaccttc gctttgttat ttgcgggcat cttacaaacg catttgcaac
     2821 gtgtgatgaa tctcggctat atgcaggtgc aatcgcaact tgaattgttt tattggttac
     2881 gcttgggttc cggggtcttt ttcattgttg gtgcgctgat attcatctac tcggtgctag
     2941 ggcccaaccg tgaagcattt gttgccggaa aattccagca ctataacgtc tgatggcaca
     3001 tgattcggtg tttgccgcag caaaaggacc agtatctgcg caatctgcta ctgaaattcc
     3061 tttctatcac gaaacaggta atgaatgtgc tttgtttgca gcagcctacc gcgaaggatt
     3121 accactacta cttaaagggc ctaccggttg tggcaaaact cgttttgttg cccacatggc
     3181 agctcgtctc ggtcgaccgc tccacactgt ttcatgtcac gatgatctaa ccgcagcaga
     3241 tttgaccgga cggtatctgc tgcaaggtgg tgagacaaca tggctagatg gcccactaac
     3301 tcgagccgtg cgtgaaggcg gtatctgcta tctagatgag attgtcgagg cgcgtaagga
     3361 tgttacggtt gttctccatc cgctcactga tgatcgccga atcctgcctc tagagcgtac
     3421 cggagaattt ttgcaagcac cggatgattt tatgttggtc gtatcttaca accccggtta
     3481 ccagaacatt ttaaaatcat tgaagcctag tacgcggcaa cgctttgtca cgatcaattt
     3541 cgattttcct cctcccatta ttgaaactag gattgttgct accgagagcg gcctagcaga
     3601 agaacggtgt cgtcttttag tcgatttggc acaacgcata cgagtgctga agaacattga
     3661 tttagaagaa gttgtttcca cgcgattgct gatttattgc gccatactgt taaaaaatgg
     3721 cttcgatccc tatcaggctg cgcaggccac gctgatcgaa ccgctatgcg aagatgatga
     3781 tgtgaaacag ggattgctgg aattggtccg cgctacattt ggttaatcta tgaattggct
     3841 taattttact gagttagagg aacaggctgg tcggttttgg catcggatcg ttggatcagc
     3901 agaaagttac ccgaactacc aacaggccgc tatcacgctg gatcaggtac gcccagcgct
     3961 gggtattttc tttcgtggac taggtggtgc ttcaggtgtt gctattcaaa gtggatcccc
     4021 acggtcatcc ggtcat
//
LOCUS       DAOQLJ010000185         4024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_11151_length_4024_cov_4.932981, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000185 DAOQLJ010000000
VERSION     DAOQLJ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11151_length_4024_cov_4.932981"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PK522_10435"
     CDS             complement(<1..436)
                     /locus_tag="PK522_10435"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNC42190.1"
                     /translation="MFNHSGMREYQLALTERINSATNAPSPAIPMLGVSIGEERWLVP
                     MNSISEVLPVPKITPVPLTHRWFLGVINVRGYLYGISDIAAYLGHALTPLTPKSRIIL
                     FAPRLGANSGLLVSNLLGIRNLNEFKQIEDHDVAHATIKARQF"
     gene            complement(441..806)
                     /locus_tag="PK522_10440"
     CDS             complement(441..806)
                     /locus_tag="PK522_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC42191.1"
                     /translation="MSIRKILVIDDSPTDRHVLRNILIENGYQVGIAESGEEGVSKIK
                     ESMPDLVLMDVVMPGMNGFQATRVLSRDETTQHIPIILCTSKGQETDKIWGLRQGARD
                     YITKPIIAEELLQKISQLN"
     gene            1046..1810
                     /locus_tag="PK522_10445"
     CDS             1046..1810
                     /locus_tag="PK522_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HNC42192.1"
                     /translation="MDFTAYFRFYAELNDFLPPIQRQQVITYRFNGHPGIKDPIEAQG
                     IPHPEVELILVNDQPVDFSYQLRDTDRVAVYPKFIQLNLSGLPRLRSELPNPVRFIVD
                     VNLGKLAKLLRLLGFDSLYCNHFQDSVIVATAIAEQRIILTRDRRLLFAKKIEHGYWI
                     RAVEAKKQTHEVLNHFELHTAIQPFSRCLTCNGLLAVMPKASIIEQLEPKTKQYYDTF
                     YQCTLCQKIYWEGSHLDHMRHDFAALLSAQPPSQQH"
     gene            complement(1813..3123)
                     /locus_tag="PK522_10450"
     CDS             complement(1813..3123)
                     /locus_tag="PK522_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNC42193.1"
                     /translation="MTMNRRKFLTTTGLTALAASCAPPTLMSQPLPNVTAHEQLKKLQ
                     HEGLQKQLLLGYPINMKTPPEAFFEWRKQLMAAGLNRFAFNNVGNPYDQSHIPFNSHQ
                     FEKALINRFAAVYGFAQDDVWGFLTNSGTDSNMHGLYMGRTLLKKRTGVLPKIYFTQE
                     AHYSIQILSDVLNLEAVIVKTDMQGNMDVADLESKLADNANQPALIVATIGTTFKGAI
                     DPIDEIQRKLRNRQAYLHLDAALFGGYLPHTPFASDLLQQGMNPEQGVARHHRYDSIA
                     VSCHKFFGFPSPAGLFITTRKIFEEFLQPFSEIHDPEYILQIPGTITCSRDAVKPAEF
                     YFFSSKSAFAQQAKDANTILENARYLYQEMKTHYANLQPTRVSEKSNIVYFRKPSDKI
                     VNKYSLAVMRVNQEGERIPCAHVVVMPHAEKSVLDQFLTDLGKS"
     gene            complement(3339..>4024)
                     /locus_tag="PK522_10455"
     CDS             complement(3339..>4024)
                     /locus_tag="PK522_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42194.1"
                     /translation="TVMASDIKKLEQKLIYAERYMMFFEIITLLLFLSVAVYAGYLIY
                     LHDWEELLNLLPSAAPLCAALLVAKSSTRLIVYYKKIDEIKHAREIIGVTHHSYAILN
                     DLRGRVQHMVSAFTNGNVPLLSIKLNAETIQKRYESLYDRDIYRYLPGDVVNDIGSLS
                     GPIFALVFTSEYFVSTFEKKGLSMLLTLAAKEISEEREQEFSKTLSQLDADLKALTTK
                     FFTIRETLD"
BASE COUNT         1131 a          900 c          806 g         1187 t
ORIGIN      
        1 caaattgcct ggctttaatc gtggcatgag ccacatcgtg atcttcaatt tgcttaaatt
       61 catttaaatt acgaatcccg agcaaattac tgaccagtaa accgctattg gcacccaggc
      121 gaggcgcaaa caagataatt ctcgatttgg gggttaatgg ggttagcgca tgacccaaat
      181 aagctgcgat atcggaaatt ccataaaggt aaccacgtac attgataacc cccagaaacc
      241 agcgatgagt gagtggaacg ggcgtaattt tcggaacagg cagtacctcg ctaatgctat
      301 tcatgggtac cagccaacgc tcttctccaa tactaacgcc tagcatcgga atcgctgggg
      361 atggggcatt ggtagcgcta ttgatccgtt ccgtcagcgc taattgatat tctcgcattc
      421 cagaatgatt gaacacgcgg ctagttcaat tgtgatattt tttgcagcaa ctcttctgca
      481 atgatcggct tcgtaatata gtcgcgcgca ccctgacgaa gcccccagat cttatcggtt
      541 tcttgtcctt ttgaggtaca aaggataatc ggtatgtgct gcgtcgtctc atccctggat
      601 aacactcgcg tagcttggaa gccattcatt ccaggcatca ccacatccat gagcactaaa
      661 tctggcatcg attccttaat tttactgacg ccttcttccc cactctcggc aatacccact
      721 tggtaaccat tttcaatcaa gatattgcgc aatacatgac gatccgtcgg agaatcatca
      781 atcactaaaa tttttcgaat tgacatgaac gttataaatt ttcgtaacgg taaataaaat
      841 aattcaaatc gaccctttgc tgttactcat tcggtgggtc gcatcgtgct acagcgctaa
      901 gcccactctt gcatcaatga tattagatat aactccattt tgcggtaaga tcaagaaaaa
      961 atgcctactg acaagcataa tcatcatttt tgttgtaaat ctgcttacta ggctgtgaat
     1021 tcagggtaat caaacgttga tctatatgga ctttacggct tatttccgct tctacgctga
     1081 attaaacgat tttcttcccc cgatacagcg tcagcaagtg ataacctatc gtttcaatgg
     1141 ccatcccggt atcaaagatc ctatagaagc acaaggcatc cctcatcccg aagtcgaact
     1201 tattctggtt aatgaccagc cagtcgattt tagctatcaa ctgcgagata ctgatcgcgt
     1261 tgcggtttat cctaaattta tccagcttaa tctttcgggg ttgccacgat tacgttctga
     1321 attaccaaac cctgtgcggt ttatcgtgga tgttaatctg ggtaagttgg ctaaattatt
     1381 gcggttgctt ggctttgata gcctgtactg taaccatttc caagattccg tcatcgttgc
     1441 aacggctatt gctgagcaac ggatcatcct gacacgcgat cgtcgcttat tatttgccaa
     1501 aaaaattgag catggctatt ggattcgcgc agtcgaagcc aaaaaacaaa cgcatgaagt
     1561 tttgaatcat ttcgaactcc atactgcaat acaacccttt tcccgttgtt taacttgcaa
     1621 tggattactc gctgtcatgc ccaaagcgag catcattgaa cagctcgaac ccaaaacgaa
     1681 acaatactac gacacgtttt atcagtgcac actttgtcag aaaatttact gggaaggctc
     1741 gcatttagat catatgcgcc atgattttgc tgcactatta tcagctcagc ccccttccca
     1801 gcaacattag cattacgatt tacccaggtc tgtcaaaaat tgatcaagca ccgatttttc
     1861 tgcatgcggc atcacgacca catgcgcgca aggaatacgt tctccctctt gattgactcg
     1921 catcacagcc agtgaatatt tgttaacgat cttatcgctg ggttttctaa aatagacaat
     1981 attcgatttt tcactcaccc gagtcggttg taagtttgca taatgcgttt tcatctcttg
     2041 atataaatag cgcgcattct ccaggatggt gttggcatcc tttgcttgtt gggcaaatgc
     2101 agattttgag ctaaaaaaat aaaactcagc cggttttacc gcatcacgcg aacaagtaat
     2161 cgtgcccgga atttgtaaaa tatattctgg atcgtgaatt tcactaaatg gttgcagaaa
     2221 ttcctcaaaa attttccgtg ttgtaatgaa cagacctgct ggagacggaa atccaaaaaa
     2281 tttatggcat gaaaccgcaa ttgaatcgta gcgatgatgt cgtgcaaccc cttgttcagg
     2341 gttcatacct tgctgcaaca aatcgctcgc aaaaggagta tgcggtaaat aacccccaaa
     2401 taaagccgca tcgagatgaa ggtaagcctg ccgattcctc agtttgcgtt gtatttcgtc
     2461 aatcggatcg atcgcgcctt taaacgtcgt accaatggta gccacgatga gtgcaggttg
     2521 attcgcattg tcagcaagtt tactttccaa atcagccaca tccatattac cttgcatatc
     2581 cgttttaacg atgactgctt caagattgag cacatcgcta aggatttgga tcgaataatg
     2641 cgcttcttgc gtaaaataaa ttttgggtaa aacaccggtg cgttttttta atagcgtgcg
     2701 ccccatgtaa agtccatgca tattgctatc cgtcccgcta ttcgtcagga acccccaaac
     2761 atcatcttga gcaaaaccgt agactgccgc gaaacgattg atgagcgctt tctcaaattg
     2821 atggctgtta aagggaatat ggctttgatc atagggattt cccacgttat tgaatgcaaa
     2881 gcgattcaat ccagccgcca tgagttgctt acgccattca aaaaacgctt caggcggtgt
     2941 cttcatatta atggggtaac ctagcagtaa ttgcttttgc aacccttcat gttgtaattt
     3001 tttgagttgc tcatgcgcgg ttacattggg taaaggttga ctcatcagcg tgggtggagc
     3061 gcaagatgct gccagtgccg tcagtccggt tgtggtcaaa aattttcttc gattcattgt
     3121 catgatattc ccctatcgtc ggttaacgat atagtggtct atatcgaatc attaatgacc
     3181 attagacgct gaaatcgctg attagttgtg cgattggcgg tgttgatttc gagtaaatag
     3241 ctatagatga ttttttgtac ctgtcttgat tctacaaaca gtctaacagg tcagtgaaaa
     3301 ctccccaata tcgtaagttt gtacaatatt cagtattact aatccagagt ttcacgaata
     3361 gtaaagaatt ttgtagttag tgctttcaga tctgcgtcta attgagaaag tgttttagaa
     3421 aactcttgtt cccgctcctc acttatctct tttgccgcaa gagttaatag cattgataac
     3481 cctttctttt cgaaggttga tacaaaatac tcactcgtaa aaaccaaagc aaaaataggg
     3541 ccggagaggg aaccgatgtc attaactaca tcacccggaa gataacgata aatatctcta
     3601 tcatagagtg attcatatcg tttttgtatc gtctcagcgt ttagcttaat ggataataac
     3661 ggtacattcc cattggtaaa ggcagaaacc atatgctgta cacgccccct taagtcattt
     3721 agtattgcat agctatgatg agtaacgcca ataatctcgc gtgcgtgttt aatttcgtct
     3781 atctttttat agtatacgat tagacgggtt gatgactttg ccacaagcag cgcagcacat
     3841 aacggtgcgg cgcttggcag cagattaagg agctcctccc aatcatgtaa gtaaatcaaa
     3901 taccctgcat aaactgctac agacaaaaac agcagtaaag taatgatttc aaaaaacatc
     3961 atgtaacgtt cagcgtaaat gagtttttgc tcaagttttt ttatatcact agccataaca
     4021 gtta
//
LOCUS       DAOQLJ010000186         4007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_11225_length_4007_cov_5.383350, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000186 DAOQLJ010000000
VERSION     DAOQLJ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11225_length_4007_cov_5.383350"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PK522_10460"
     CDS             complement(<1..553)
                     /locus_tag="PK522_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42195.1"
                     /translation="MTTTIETAFHHLDQLADLLRAEGFACGPDVWLSVYRLLAQFKRK
                     NKLPHDTDQLRLLLGPLFCRNPEEQARFKAIYERWWTIHTQATSADYQKDGQSDAFTL
                     SAPNRLSAPGQAALIALRQKIKQVGRKWMFAIFAVIAVVGTLIYFSLKYPTPPVTTPP
                     QKQLTVPSKPDGDKQPSTQALPST"
     gene            complement(564..1697)
                     /locus_tag="PK522_10465"
     CDS             complement(564..1697)
                     /locus_tag="PK522_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNC42196.1"
                     /translation="MTTTSDGDYYTGKGEQKESSAWQNLPSFDPDELTHPKDYLAPAG
                     LAAAVNVALELGLPLLLTGEPGCGKSQLAYSLAWELGFPTQPLSFTVKSDTQSKDLFY
                     HFDTLGRFRAARIEDEEADARRYLRFEALGLAILHALGPDRIHALGLTSCLETLPPQP
                     QRSVVLIDEIDKAPREIPNDILNEIDRMSFDIPEIFQAGKARTVVSLSNHDNHGKQNL
                     LRPIVIITSNRERELPEAFLRRCVYYHLDIPPFRRQLTNGSNPDQGITLEQIVEKRLG
                     FEFDSRPDQPNNQKNLWNAAISLFDYLRNEIVLQKNPSTAELLNWIQLLKKRGGSPQL
                     TLSEASWRNLFVSSAKVTLFKHQEDQNRAQQLIEKWLELPVTA"
     gene            complement(1708..2937)
                     /locus_tag="PK522_10470"
     CDS             complement(1708..2937)
                     /locus_tag="PK522_10470"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNC42197.1"
                     /translation="MKDFFISYNSADEDDATWIAWQLEETGYTVIIQAWDFKKGNNFV
                     IEMNKASIAAKQTIAILSPNFLASQYTLPEWAAAFAQDPTGTQRKLIPVRVKPVEITS
                     GLLAAINYIDLVGIDRETAKQKLLQGVKGERLKPATEPTPSFHLQPNEKIKAPNNFAI
                     AEYKIGQLNRTTQYKHFKAHIPKECCLQQGKPFGFILAGPEQEWPVAIRFRLAYLLEK
                     VLIGDSKHMPNFVRLNTDLGLANEPVENDNQYLWDLLGDALNCQREQTALQEKLSQLP
                     ECHIFFREALQEEINAPNFLANLLSAWKNLQLHPSSKSHFLLFICEIDNPQQSWQEQI
                     ASALQQSNIEQALLPQLQSLNYHGDIKDWIKLNIDNDTLRDSISEALAEFAQQADIPL
                     IKLKKILKPLIERYPSH"
     gene            complement(3067..>4007)
                     /locus_tag="PK522_10475"
     CDS             complement(3067..>4007)
                     /locus_tag="PK522_10475"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNC42198.1"
                     /translation="LTTLSFYEYLQLKTITLPTLPTLRSLKELFTWSPNELRHISEQA
                     QPYIGHFHNYLVYGGFPQTALVKSVTQAQKLLREDIIDKAIKRDMTALFGVRRILDLE
                     HTFLYLCRHDGGLLDLTALSSNLKVTRPTAQHFIDLLEAAHLVYRLAPFGYGKEVLRG
                     RYKIYLADAAIAPAVMLKGQSIIDDPDALGIAAEASVLKHLFARYYQQNVRFTYWRGK
                     KELEVDLVAEIAGQIIPFEVKYRSQPVDARSLKGLIAFCHEKSIKRGYIITKSLDDLG
                     LLPQKEENAVPLFRIPATLLCFWMGEMELRQQQEIE"
BASE COUNT         1016 a          791 c         1001 g         1199 t
ORIGIN      
        1 gtgtggaagg taaagcttgg gttgacggtt gcttatcgcc atctggttta gatggaacgg
       61 taagctgctt ttggggtggc gtggttaccg gtggtgtcgg atatttcagg gaaaaataaa
      121 tcaatgtgcc aacgaccgcg atcacggcaa agatcgcgaa catccattta cggccaactt
      181 gcttgatttt ctggcgcaat gcgatgagcg ccgcttgccc tggagcgctt aagcgattgg
      241 gggcagaaag ggtaaaggca tcgctttgcc catccttttg ataatccgca gacgttgctt
      301 gggtgtgtat ggtccaccaa cgttcataaa tcgctttgaa acgcgcttgt tcttcagggt
      361 tgcgacaaaa gagagggcct aataataacc ttaactgatc ggtatcgtgc ggtagtttat
      421 tttttcgttt gaattgcgct agcagtcgat aaacggaaag ccaaacatcg ggtccacaag
      481 cgaatccttc cgcacgtaag agatctgcaa gctgatctag gtggtggaaa gcggtttcaa
      541 tggtcgttgt cacagcgatt gaatcaagcg gtcactggga gttctagcca cttttcaatt
      601 agttgctgcg ctctgttttg atcttcttga tgtttaaaca gcgttacttt ggcgctgctg
      661 acaaacagat ttcgccaaga tgcttcgcta agcgttagtt gcggagagcc accgcgtttc
      721 ttcaagagct gaatccaatt taacagttct gcggttgagg gattcttttg tagcacgatt
      781 tcattgcgta gataatcaaa aagcgaaatg gcggcattcc agagattttt ctggttattg
      841 ggttgatcgg gtcttgaatc gaactcaaac cctaagcgtt tttcaacaat ttgttccaag
      901 gtgatgccct gatctgggtt actaccattc gttagctgcc tccggaaagg cggtatatcc
      961 agatgataat aaacacagcg acggagaaat gcttccggta attcacgttc gcgattactg
     1021 gtaatgatga cgatggggcg caataagttt tgctttccgt ggttgtcatg attactgagc
     1081 gatacgacgg tgcgtgcttt tcctgcctga aaaatttcag gaatatcgaa gctcatgcgg
     1141 tcaatttcgt tgaggatatc gttgggaatt tcacggggtg ctttatcgat ttcatcgatg
     1201 agcacgactg aacgctgggg ttgcggcggc aatgtttcca ggcaagaagt caatcctaat
     1261 gcgtgaatgc gatctgggcc taacgcatgt aagatggcga gtccaagcgc ttcaaaacgg
     1321 agataacggc gtgcatcggc ttcttcgtct tcgattcttg cagcgcggaa acgccctaag
     1381 gtatcgaagt gataaaacaa atctttgctt tgggtgtcgg atttaacggt aaaacttaag
     1441 ggctgtgttg gaaaccctaa ttcccatgcg aggctataag cgagttgaga tttgccacac
     1501 cccggttcgc ctgttaatag caagggtaaa ccgagttcta atgcgacatt gactgctgcg
     1561 gcaagtccag caggtgccaa gtaatcttta ggatgcgtga gttcatcggg atcaaagctg
     1621 ggtaagttct gccatgcgga tgattctttt tgctcacctt taccggtgta ataatctcca
     1681 tcggaagtgg ttgtcatggt ttctccatta atgagatggg taacgttcta tcaaaggttt
     1741 tagaattttt tttagtttaa tcaagggaat atccgcttgc tgcgcaaatt ctgctagcgc
     1801 ttcgctaatg gagtcgcgta acgtatcatt atcgatatta agcttaatcc aatctttaat
     1861 atcaccgtga taattgaggg attgaagctg aggtaacagc gcttgttcta tattagactg
     1921 ctgcagcgca gatgctattt gttcttgcca ggattgctgg gggttgtcaa tttcgcagat
     1981 aaataataga aaatgacttt tggagctggg gtgcaattgc aggtttttcc aagcgcttag
     2041 gaggtttgct agaaaattag gtgcgttaat ttcttcttga agcgcttcgc gaaagaaaat
     2101 gtggcattca ggcagttgac ttaatttttc ctgaagtgcg gtttgctctc gttggcaatt
     2161 gagcgcatca cccaataaat cccataaata ttgattatcg ttttcgactg gttcattggc
     2221 taaacccaag tcagtattta atctcacgaa gttgggcata tgtttggagt caccgattaa
     2281 aactttttcg agtaagtaag ctaagcgaaa tcgaatcgcg acaggccatt cttgctcggg
     2341 tccagctaat ataaaaccga agggttttcc ctgttgaaga cagcattctt tggggatatg
     2401 cgccttaaaa tgcttgtatt gagtcgttcg atttaattgg ccaattttat attctgcaat
     2461 agcaaaatta tttggagctt ttattttttc gttcggttgt aggtgaaagc ttggggtcgg
     2521 ttccgtggct ggttttaacc tttctccttt aacaccttgc aataattttt gtttggcggt
     2581 ttcgcggtct ataccgacta aatcaatata attgattgcg gctaacaaac cactggtgat
     2641 ttcaactggt tttactcgga ctggaattaa ttttcgctgc gtaccagttg gatcttgtgc
     2701 aaatgctgct gcccattccg ggagagtata ttgagacgct agaaaattag gagagagaat
     2761 tgcgatagtc tgcttggcgg ctatgcttgc tttattcatc tcgataacaa aattattccc
     2821 cttcttaaaa tcccacgcct gaataataac tgtataacca gtttcttcaa gctgccaggc
     2881 aatccaggtt gcgtcatctt cgtctgcact gttatagctg atgaagaaat ctttcatgac
     2941 tgattctttt tattattagg taaataataa tgaatctatc ataactgtgg gcggattcaa
     3001 gtcatatcgc gggatgttcg agaaaatcac ttggaaaatt atgtggaagt gttgggcgga
     3061 gaaaatttat tcgatttctt gttgctgacg taactccatt tctcccatcc agaagcagag
     3121 gagggtagca gggatgcgaa atagcggcac tgcattttct tctttttgcg ggagcagacc
     3181 taaatcatca agggatttgg taatgatata gccgcgcttg atacttttct catggcaaaa
     3241 cgcaatgagt cccttgaggc tgcgtgcatc aacgggttgt gaccgatatt taacttcaaa
     3301 tgggatgatc tgaccggcaa tttcggcaac taaatcgact tccaattctt tctttccacg
     3361 ccaataagtg aagcgtacat tttgctggta atagcgcgca aaaagatgtt tcaaaactga
     3421 tgcctcggca gcgataccca gggcatcagg gtcgtcgatg atcgattgtc ctttcaacat
     3481 cactgcaggg gcaatcgccg catcagcgag atatattttg tagcggccgc gcaatacttc
     3541 tttgccatag ccgaaagggg ccagacggta aacgagatga gccgcttcaa gtaagtcgat
     3601 aaagtgctga gccgttgggc gtgtcacttt aagattgctg cttaatgcgg ttagatcgag
     3661 taagccacca tcgtgacggc atagatataa gaaagtatgt tcaagatcaa gaatgcgcct
     3721 tacaccaaaa agtgcggtca tatcgcgttt gatcgctttg tcgatgatgt cttcgcgtag
     3781 taatttttgc gcttgtgtga cgcttttcac tagcgctgtt tgaggaaaac cgccataaac
     3841 cagataattg tgaaaatgtc cgatatacgg ttgggcttgc tcactgatat ggcgcaactc
     3901 gttgggggac caagtaaata gttccttgag cgagcgtaag gttggtagtg ttggaagcgt
     3961 tatggttttg agttgtaagt attcatagaa ggatagcgtt gttaggc
//
LOCUS       DAOQLJ010000187         3964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_11448_length_3964_cov_6.458429, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000187 DAOQLJ010000000
VERSION     DAOQLJ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11448_length_3964_cov_6.458429"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2146
                     /gene="purL"
                     /locus_tag="PK522_10480"
                     /gene_synonym="purI"
     CDS             <1..2146
                     /gene="purL"
                     /locus_tag="PK522_10480"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNC42199.1"
                     /translation="QRTAVHVGNPLKPIDFSHIQLKDAIYRILRLPTVADKSFLITIG
                     DRSIGGMTARDQMVGPWQIPVADVAVTAMGYQTHLGEAFAIGERTPLALINAAASARM
                     AIGEALTNIAAASIERLDYIKLSANWMAAAGHANEDAALFDAVHAAAIALCPQLGISI
                     PVGKDSMSMKTTWWQENNVGENQKKEVIAPLSLIISAFARVKDVRQTLTPQLRNDLGE
                     TALILIDLGFGKNRMGGSALVQVYNQLGDQAPDLETNPEIEKFKSFLGVIQQLKESNQ
                     IVAYHDRSDGGLFVTLCEMVFAGHIGVTINLDQLCFDPSASDLDGAEIKPEALQGRFF
                     ERLLSVLFNEELGAVIQIQDVHHQAVLQAFAEVGLANHCFVIGKLNQSDEIQILRNNI
                     AVIREKRVDLHRAWSETSYHMQRIRDNPACAQQEYDRILDTADPGLHSKLTFNVHDDI
                     AFPSIQMRSRPKIAILREQGVNGQVEMAAAFDQASFSAVDVHMSDILAGRDLLTDYKG
                     LVACGGFSYGDVLGAGKGWAKSILFNPVARDAFQTFFNRQDTFALGVCNGCQMMSNLE
                     EIMPGTAHWPRFERNQSEQFEARFILVEIPESPSILFKGMEGSRLPITVAHGEGFASF
                     QTSQQLATASHYIALRYIDHYGNPTERYPFNPNGSALGITGLTSVDGRFTILMPHPER
                     VFRTIQHSWHAEDWPTYSPWMRMFRNARQWVG"
     gene            2221..3489
                     /locus_tag="PK522_10485"
     CDS             2221..3489
                     /locus_tag="PK522_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNC42200.1"
                     /translation="MTNNKNKHKLPLNLSMRVWFALIVILSSNASAEIIPLVKPIQTS
                     RAASSTKQCVPVASWVIPGKNVKTSFTEIASHANQHSVILLGETHVNREHHRWQLQML
                     AALHTIRPDMIIGFEMFPRSAQKALDQWIAGELSESDFLQKTNWNSVWGTDASLYLPL
                     FHFARMNRIPMRALNIDSELRRRVSEKGFDSVPENEREGVTRPAPPSAAYLDFLLPIY
                     ELHDRKHKQQAKSNKYDPDFLRFVVGQQLWDRAMAQEIKAALDLLEKRKNSLVVGIMG
                     TGHILHGFGVPHQLKDLGVNGITTLIPWDTNKSCKQLTDGYADAVFGLTPQNIEVPPS
                     FQRLGIRYEMMVEQGGARVMEVEKASIAESAGLLTGDIIREIAGVAVKDTNDIVTVVK
                     RQAPGTWLPISIERGKELKQIIAKFPALAK"
     gene            complement(3713..>3964)
                     /locus_tag="PK522_10490"
     CDS             complement(3713..>3964)
                     /locus_tag="PK522_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HNC42201.1"
                     /translation="GSTIIPLNILMNTEIEKADLEAFLRSLTDERVRYRKAPFDHPQL
                     FIPVGYKPDSNSAKNIKEIFLEIEAVGREGGKPLKPFLR"
BASE COUNT         1129 a          786 c          909 g         1140 t
ORIGIN      
        1 gcagcgaact gccgtgcatg ttggtaatcc actcaaaccg atcgatttct ctcatatcca
       61 attgaaagat gcgatttatc gaattctaag attaccgact gttgcggata aatccttctt
      121 gatcacgatt ggtgatcgca gtattggggg tatgactgcc cgtgaccaaa tggtaggtcc
      181 atggcaaatc ccggttgccg atgttgcggt gacagcgatg gggtatcaaa ctcacttagg
      241 ggaagctttt gctatcggtg aacgaactcc cctggcactc ataaatgcag ccgcttctgc
      301 tcgaatggcg attggcgaag cgctaactaa tatcgctgct gcttcgatcg aacgattgga
      361 ttatattaaa ctgtcagcca attggatggc tgctgccggt catgcaaacg aggatgctgc
      421 gttgtttgat gcagttcatg ctgcagcgat agcattgtgt cctcaactgg gtatcagtat
      481 tccagttgga aaagattcta tgtccatgaa aacgacttgg tggcaggaaa ataatgtggg
      541 tgagaatcag aagaaagaag tgatagcacc cttatcattg atcatttctg cttttgcacg
      601 ggtcaaggac gttcgtcaaa cactaacacc gcagctgcgc aatgatctag gagaaacagc
      661 gctcatttta atcgatttgg gatttggaaa aaatcgaatg ggaggttctg cactagtcca
      721 agtttataat caattgggcg atcaagcgcc agatctagaa actaaccctg aaatagaaaa
      781 atttaagtca ttcttgggtg tgattcagca gctcaaggaa tcaaatcaga tcgtggctta
      841 tcatgatcgt tccgatggtg ggctttttgt aacattgtgt gaaatggttt ttgctgggca
      901 tatcggggtt accattaatt tggatcaact ctgctttgat ccatcggcaa gtgatcttga
      961 tggtgctgaa ataaagccag aagccctaca aggtcgcttc tttgaacgct tactgagcgt
     1021 attgttcaat gaagaattag gtgcggttat tcaaattcag gatgtgcatc atcaagccgt
     1081 attgcaagct tttgctgaag ttgggttggc taaccattgt tttgtaattg gaaaattgaa
     1141 tcaatctgat gagattcaaa ttttacgcaa caacatcgcc gttattcgtg aaaaacgtgt
     1201 cgatttgcat cgcgcatggt cagaaacgag ttatcacatg caacgtatcc gcgataatcc
     1261 agcgtgtgct caacaagaat atgatcgtat tttagatact gctgatccag ggctgcattc
     1321 aaagctaact tttaatgtgc acgatgatat tgcatttcca tcgattcaaa tgcgatctcg
     1381 ccctaaaatc gctattctgc gcgaacaagg tgtcaatggg caggttgaaa tggctgctgc
     1441 ttttgatcaa gctagttttt cagcagttga tgttcacatg agcgatattc ttgctgggcg
     1501 tgatttatta acagactata aaggcctagt cgcttgtggt ggcttttcat atggtgatgt
     1561 tctaggcgca gggaaaggtt gggcgaaatc gattttattt aaccccgttg cacgcgatgc
     1621 attccaaacc ttttttaacc ggcaagatac ctttgcttta ggcgtttgca acggttgtca
     1681 gatgatgagc aatctagaag aaatcatgcc aggtactgcg cactggcctc gttttgagcg
     1741 taatcaatct gaacagtttg aagcacgctt tatattagtt gaaatacccg aaagcccttc
     1801 tattttgttt aaaggcatgg aaggcagtcg attaccgatt actgtcgcgc atggtgaggg
     1861 atttgcttcg tttcaaacct cgcagcaatt agcaacagca tctcattata ttgctttgcg
     1921 ctatatcgat cattatggta atccaactga gcgataccca tttaacccca atggttcggc
     1981 cttaggtata actggcttaa cctcagtgga tggtcgattt actattttaa tgccacaccc
     2041 agaaagggta tttcgtacaa ttcagcattc ttggcatgct gaagattggc caacatattc
     2101 accttggatg agaatgttta ggaatgcccg gcaatgggta gggtaaccgt tgaactaatt
     2161 gcgtgttccg tgagtcatcc ttattaactc atttacaata accctcaaag aaattaatcg
     2221 atgaccaata ataaaaataa gcataagtta ccacttaact tatcaatgcg cgtatggttt
     2281 gccctcattg ttattttatc ttcaaacgca tccgctgaaa ttatcccact cgtcaaaccc
     2341 attcaaactt cacgagcagc ttcatccact aaacaatgtg ttcctgtggc aagttgggtg
     2401 atacctggga aaaatgtaaa gacttctttt actgagattg cttctcacgc taatcaacat
     2461 tctgtcattt tactcggtga aactcatgtt aaccgtgaac atcatcgttg gcagctacag
     2521 atgcttgctg ctttgcatac gatacgtccc gatatgatca ttggctttga gatgttcccc
     2581 cgaagtgctc agaaggccct tgatcaatgg atcgctggtg aattatcgga atcggatttt
     2641 ttacagaaaa caaactggaa ttcagtctgg ggaacggatg caagccttta tttgccgctg
     2701 ttccattttg cccgcatgaa tcgcatacct atgcgagcgc ttaatattga tagcgagcta
     2761 aggaggcgag tttccgagaa aggttttgat agcgtaccgg agaatgagcg cgaaggagtt
     2821 actagacctg caccaccaag tgctgcttat cttgattttc ttttaccgat ttatgagtta
     2881 catgatcgta agcataaaca acaagctaaa tctaataagt atgaccccga ttttttaagg
     2941 tttgtagtcg gtcagcaatt atgggatcgt gcgatggccc aagaaattaa ggcagcgttg
     3001 gatttacttg aaaaacgcaa aaattctctg gtagtgggta tcatgggaac agggcatatt
     3061 ctgcatggtt ttggtgtgcc tcatcaatta aaagatcttg gagtcaatgg cataaccact
     3121 ctgattcctt gggatacgaa caaatcctgt aaacaattga ccgacggtta tgccgatgca
     3181 gtgtttggat taacacccca aaatattgag gtgcctcctt cctttcaacg gctaggtatt
     3241 cgctatgaaa tgatggtaga gcaaggaggg gcccgtgtta tggaagttga aaaagcgagt
     3301 attgctgaat cggcagggct gctgacaggg gatattatcc gtgaaattgc aggggtcgca
     3361 gtgaaggata caaatgatat cgttaccgtg gttaaacgtc aggcaccggg tacatggcta
     3421 ccgattagca tagaacgcgg aaaagagcta aagcaaatca ttgcaaagtt tccggcgcta
     3481 gcgaagtaac gcattggaat cattaatgac aggtttaaga gaaaaagaga ttaaatcaac
     3541 cggaactaaa taataatttc cagttgattt aatcaacctc tacttaagag gaaaggataa
     3601 gagaagctat gaaaaacata cgctatgata tgcttgagta aagtaataaa tcaatgtgac
     3661 atattgtcgc gtaggcaaga aacgaaagtt aatcgatgac acacaatgaa actcagcgta
     3721 aaaatggttt tagtggtttt cccccttcac gaccgactgc ctcaatttct agaaaaattt
     3781 ccttaatatt ctttgcgcta ttagaatctg gcttataacc gacaggaata aaaagctgag
     3841 gatgatcgaa aggtgctttt cgatagcgga cacgctcatc ggtgagcgat cgtaaaaatg
     3901 cttccaaatc agctttttct atttccgtat tcatgaggat attcagcgga atgattgtag
     3961 agcc
//
LOCUS       DAOQLJ010000188         3954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_11506_length_3954_cov_4.111311, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000188 DAOQLJ010000000
VERSION     DAOQLJ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11506_length_3954_cov_4.111311"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PK522_10495"
     CDS             <1..236
                     /locus_tag="PK522_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42202.1"
                     /translation="AWPDYKLAVEIEGGVWIKGRHTSGSGFMKDMEKYNALTMLGWSL
                     LRYTPKQTSYQKTVNEIFELIEILKSPKSSFLA"
     gene            267..587
                     /locus_tag="PK522_10500"
     CDS             267..587
                     /locus_tag="PK522_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42203.1"
                     /translation="MQSKEWIIKRDIAAEKIGHITNLPPQYWLATKYLKYKHSNGKDE
                     YPCERLLDLLNKIAYNKPLAYVIAAVKNDVTKWRNEAVNSVPESNELIKQLTERMKYV
                     KRNA"
     gene            568..1008
                     /gene="ssb"
                     /locus_tag="PK522_10505"
     CDS             568..1008
                     /gene="ssb"
                     /locus_tag="PK522_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNC42204.1"
                     /translation="MSKGTLNKAIIIGRLGKDPTLKYLPSGDAVASFGVATNDNYKDK
                     EGKIVENTDWHNVSAFGKLAEICGQYLKQGSLVYVEGKLKTRKYTTKDGIEKTATEIK
                     AESMQMLGDKKEVTVSQTETHGREAQVPNLNQKDSTDQRDDLPF"
     gene            1019..2296
                     /locus_tag="PK522_10510"
     CDS             1019..2296
                     /locus_tag="PK522_10510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNC42205.1"
                     /translation="MDTTELEQALLAAYIIDDGRSVELTAEYIKADDFTKESHRKIYL
                     ACIDLWSRNEAIDALSVCSKLDKLGLLQYVGGAAYVVGLSNKLPSASNSESYAKTIAD
                     ESRKRKIKQKCRETEVKVDSSDSSNEIIDEIENWASELRFNINKEPSLYDIGKQILDD
                     AETAQQLGFNPSVVDMGFGVHYRKGTLNILAARPGMGKTTLMLNMAYNMAKKNEPVGV
                     LHLEMSSEQLTEKLLAIDSKVDVGKLKSGVFGNREIELVKASVERLPTLPIYQMESSG
                     VNELELTARAKIMKRKHNIKALFIDYMQLIGCSKFYGNKVVEVGVVSKQCKEIAKSID
                     VPVIVLSQLSRMLETRKEKRPTLSDLRDSGNIEQDADTVKFIHREDYYADKKNMPEVS
                     LCEIITEKDRFGGLSTRELMFNKPYSLFTEVER"
     gene            complement(2541..3026)
                     /locus_tag="PK522_10515"
     CDS             complement(2541..3026)
                     /locus_tag="PK522_10515"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNC42206.1"
                     /translation="MDRPNGKSGIIEVFGNPYEWIRPDGSIDPTWEMRNMTSIPISVA
                     MPLSWDKTKIVSSIYGHKLMAPIFKKVFQDIVTKGVAKYIKSYGGCYNYRPKRGQSNL
                     STHTWGIAIDLNPETNGLGVQPDISLDLVKVFEDNGFIWGGRWTGRNVDGMHFQYCSG
                     Y"
     gene            complement(3019..3429)
                     /locus_tag="PK522_10520"
     CDS             complement(3019..3429)
                     /locus_tag="PK522_10520"
                     /inference="COORDINATES: protein motif:HMM:NF025717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HNC42207.1"
                     /translation="MKYIYLSHPISKGNQFANIHISAKAYIELMNMGFIVYWPGGSAL
                     CEMIAPQHYESWMAQDKAWIERCDAVVRLAGESNGCDREVLHAKENGIPVFYGVGELQ
                     KFMENQSKDIESVIEKLNTKVLPGEELLIGDVDG"
     gene            complement(3426..3716)
                     /locus_tag="PK522_10525"
     CDS             complement(3426..3716)
                     /locus_tag="PK522_10525"
                     /inference="COORDINATES: protein motif:HMM:NF040395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6378 domain-containing protein"
                     /protein_id="HNC42208.1"
                     /translation="MKASEIADELVDTDRQKVYGHPYDDFGRIAQMTRPIIEAVVNGK
                     LDGKLGHALYMQCVKICRLIETPDHDDSIVDNHGYANTYEMVLEKMKERREL"
     gene            complement(3713..3913)
                     /locus_tag="PK522_10530"
     CDS             complement(3713..3913)
                     /locus_tag="PK522_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42209.1"
                     /translation="MKPSDDLKTDVYKGKDAQTISEISKECGLSETTVRRLASMMVSN
                     GKWKVVKIKTKVGFAKAYLRVK"
BASE COUNT         1282 a          760 c          799 g         1113 t
ORIGIN      
        1 acgcatggcc tgattataag ttggccgtag aaatagaggg aggggtttgg ataaaggggc
       61 ggcatacaag tggatcggga tttatgaaag atatggagaa atataatgcc ttgacaatgc
      121 ttgggtggag ccttttaaga tacacgccca aacagacttc ttatcaaaag actgtcaatg
      181 agatatttga gcttatcgaa attcttaaat cgccaaagtc cagtttttta gcataaaaac
      241 atgacaaatt aaaactaatt actactatgc aaagcaagga atggataatt aaaagagata
      301 tagccgctga aaagatagga catatcacta acctgcctcc acagtattgg cttgccacaa
      361 agtatctaaa gtacaagcat agcaacggaa aggacgagta cccatgcgaa agacttcttg
      421 atttgttgaa taaaattgct tataataagc cactagctta tgtaatagcg gcagttaaga
      481 atgacgtaac taagtggaga aatgaagctg tgaatagtgt ccccgaatca aatgagttaa
      541 tcaaacaact aactgaaagg atgaagtatg tcaaaaggaa cgcttaataa ggctataata
      601 attggccggc taggtaagga cccaacacta aaatatctac caagtggaga tgccgtagcc
      661 tcttttggag tggctacaaa cgataactac aaagacaagg aaggaaagat cgttgaaaac
      721 acggattggc acaatgtatc ggcttttggt aagttagccg agatatgtgg gcaatatcta
      781 aagcagggct cattggtcta tgtcgagggt aagttaaaga cccggaaata cacaacaaaa
      841 gacggaatag aaaagactgc tactgaaatt aaagcagaat caatgcaaat gcttggcgat
      901 aaaaaggaag taactgtttc ccaaacggaa acacatggac gcgaggcgca agttcctaat
      961 ttgaatcaaa aagacagtac ggaccaacga gacgacctac cattttaaca actgagtaat
     1021 ggacactaca gagcttgaac aggcgctttt agcggcgtat ataatagatg acggcagatc
     1081 ggtagagcta actgctgaat atattaaggc ggacgacttt acaaaagaaa gtcaccgaaa
     1141 gatatatttg gcgtgtatag acctttggtc tagaaatgaa gcgattgacg ctttaagcgt
     1201 ttgttcaaag cttgataagc tcggtttatt gcaatatgtt ggaggagccg cctatgttgt
     1261 aggtctttct aataagttgc cgtctgcatc taattctgag tcatacgcta aaacaatagc
     1321 tgatgaatca aggaaaagaa agattaagca aaagtgcagg gagacggagg ttaaggttga
     1381 ttcgtcggat tcgtcaaacg aaatcattga cgagatagag aactgggcgt ctgagttaag
     1441 atttaatata aacaaggagc cgtctttata tgacattggc aaacagattc ttgatgatgc
     1501 cgaaaccgcc caacaactag gatttaatcc ttctgttgtt gatatgggtt ttggcgttca
     1561 ttatcgcaaa ggcactttaa atatattagc cgccagaccc ggtatgggca agaccacgct
     1621 aatgcttaat atggcttata acatggctaa aaagaatgag cccgtaggag tgcttcatct
     1681 cgaaatgtca tcagaacaac taaccgaaaa gctattagcc atagactcaa aggttgatgt
     1741 gggcaagcta aagtctggag tatttgggaa tagagagatt gaacttgtga aggcaagtgt
     1801 agaaagactc ccgacattac cgatctatca aatggaatca tctggtgtaa atgaacttga
     1861 actaacggca agagctaaga taatgaagcg taaacacaat atcaaagctc ttttcattga
     1921 ctatatgcaa ctaataggat gttcaaagtt ctatggcaat aaagttgttg aggttggtgt
     1981 agtctctaaa caatgcaagg aaatagcgaa gagtattgac gtcccggtta tagtactatc
     2041 ccagctttcc cgtatgcttg agacgcgaaa ggaaaagcgt ccgacactgt ctgacctaag
     2101 ggactcagga aatatagagc aagatgccga taccgtcaaa ttcattcatc gtgaagatta
     2161 ttatgccgac aaaaagaata tgcccgaagt ttcactttgt gagataataa ccgagaaaga
     2221 tagattcgga gggttaagca caagagagct aatgtttaac aaaccatatt cattatttac
     2281 agaggtagag agatgataca aaatagagac ttaggcgatg agaatgatta tatacctaaa
     2341 gataaattta attatatgtc caacggacta ggtaacacat tttgtatgaa atattataag
     2401 gattttaact cagataaaat agttggatac tatttccaaa ggaatgacca cggtatatat
     2461 tgttgttata tggatattga tgaatacgga aattactcag gcgaaataaa atacaaacta
     2521 attaaaccaa taggattgta ttaatatcca gagcagtact gaaagtgcat tccatcaaca
     2581 ttgcgaccag tccaacgacc gccccatata aatccgttat cttcaaaaac cttgacaaga
     2641 tcaaggctta tatctggctg aacgcctagt ccattagtct ccggattaag gtctatagct
     2701 atgccccaag tgtgtgtaga taggttggat tgaccacgtt tgggcctgta attataacaa
     2761 cctccgtatg attttatgta cttagccact ccttttgtaa caatatcttg aaacaccttc
     2821 ttgaatattg gagccatcaa cttatgacca taaatactac taacgatctt cgttttgtcc
     2881 catgacaatg gcatcgcaac cgatattggt attgatgtca tattgcgcat ttcccatgta
     2941 ggatctattg agccgtctgg acgtatccat tcatagggat taccaaagac ctcaattata
     3001 cctgattttc catttggcct atccatctac gtctcctata agcaattctt ctcccggcaa
     3061 tacttttgtg tttagctttt ctattacaga ctcaatgtct ttagactgat tttccatgaa
     3121 tttctgcaat tcgcctaccc cataaaatac cggtattccg ttttctttgg cgtgtaatac
     3181 ctccctatca caaccattcg attcacctgc aagccttact acggcatcgc atcgttcaat
     3241 ccatgccttg tcttgtgcca tccacgattc atagtgttga ggagcgatca tttcgcataa
     3301 agcagaacca cctggccaat aaacaataaa acccatgttc attagttcaa tgtaagcttt
     3361 tgcggatatg tgaatattgg cgaactgatt ccccttgctt attggatgag atagataaat
     3421 gtatttcata attcacggcg ctccttcatt ttttcgagaa ccatttcgta tgtattggca
     3481 tatccgtggt tgtcaacgat tgaatcatca tggtccggcg tttctattaa cctacaaatt
     3541 ttgacacatt gcatatacag ggcgtgcccc agtttaccgt ctagcttacc atttactaca
     3601 gcctcaataa ttgggcgagt catttgagct attctaccga agtcatcata aggatgacca
     3661 tacacttttt gtctgtctgt gtccactaat tcatcggcaa tttctgatgc tttcatttta
     3721 ccctcaaata tgctttggca aatcctacct tagtttttat cttgacaacc ttccatttac
     3781 cattagaaac catcatagac gctaaacgtc taaccgttgt ctcactgagt ccacactctt
     3841 tacttatctc tgaaatggtt tgcgcgtcct tacctttgta aacatcagtt tttaagtcgt
     3901 cagatggttt cattagaaac tactcccacg cttatctatc ttatcaatga gatc
//
LOCUS       DAOQLJ010000189         3915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_11684_length_3915_cov_4.317876, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000189 DAOQLJ010000000
VERSION     DAOQLJ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11684_length_3915_cov_4.317876"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PK522_10535"
     CDS             complement(<1..737)
                     /locus_tag="PK522_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNC42210.1"
                     /translation="MSTDLEQFYEIFFEESSELLADMEACLLRLDVNSPDLEDLNAIF
                     RAAHSIKGGAGTFGFTDMTEMTHMLETLLDRLRKGELAVRSEMVDAFLRAGDVIKAQL
                     AGHRGEGEADPTVAAEVCEELKRLSDETQAPVISEVVEDSGTKEEAMLVEAASHKPSN
                     VIINEAAPQNDLPKLTYSIEFSSAGMGDVAVENLLANLRRLGDLENSAPASEKERFKL
                     RLTTASSEEDVWETLAFVVDPANLKIEV"
     gene            complement(875..1240)
                     /locus_tag="PK522_10540"
     CDS             complement(875..1240)
                     /locus_tag="PK522_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC42211.1"
                     /translation="MAKTILAVDDSASIRQMVTFTLKGAGYEVIQAVDGQDALDKANY
                     HQVNLVLTDINMPRMDGLKLLELLRKLAHYKNIPILILTTESGHEIKAKGRAAGATGW
                     LVKPFDPKRLLEVIGKVIG"
     gene            complement(1298..1882)
                     /locus_tag="PK522_10545"
     CDS             complement(1298..1882)
                     /locus_tag="PK522_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42212.1"
                     /translation="MAEMQTAGGESDNQVTHQSSMIEQDGVLCNAEADEYFMSIKGEL
                     NQVDRLVSDAVNNLVINFGYISKLTKSHHEMVLAIEKMAAPEGSKPILELLEKQMVIA
                     DKIEQELEMVVTSLQFGDLVTQLLAHTSRQVEALNIELQRIERQRGWRENVGKNTLDT
                     IHEDVSKAVNMAKTKSKRKPVVQQGMQMGDIELF"
     gene            complement(2174..3412)
                     /locus_tag="PK522_10550"
     CDS             complement(2174..3412)
                     /locus_tag="PK522_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNC42213.1"
                     /translation="MEGFFLGRQPILDRNQNLVAFELLFRQEETEESINIKNDLSASA
                     NVIINAYCQLGIQNVLGKQRGFINANPELVMSDIISLLPSKHVVLEIKETATVTPEFM
                     QCCNELKQKGYQFALDNIVEMNSKVEQLLPLVSVVKVDVLALEQNALAKLVAELNRWP
                     VLLLALKVENREQEKYCMQLGFQMFQGYYFARPEVLSIKRADPGKLSLLKLLTLVMGD
                     SDIEELEREFKHQPGLSYNLMRMVNSVASGLPQKINSIKHAIMILGRKQLQRWIQLLL
                     YTSNQSDDSMSNALMQTAAARGKMMELVALAERPHDKNHQERAFMVGILSLLDVLLGI
                     EMQQIVDKLGIPDDMSQALLTREGRLGQELRLIEANEKGEVAAIQLILTELSFLSLSE
                     LADIEIQAIAWANRIGEAAN"
     gene            complement(3574..>3915)
                     /locus_tag="PK522_10555"
     CDS             complement(3574..>3915)
                     /locus_tag="PK522_10555"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC42214.1"
                     /translation="HQYGDSVLINLVTVAKETLRPSDIVARFGGEEFVILLPDVEMQD
                     ALTIIQRIQNNLAKNFSIQSDSQALPITFSAGVALRSFGEHQNSVISRADKALYQAKR
                     TGKNRAIPALI"
BASE COUNT         1067 a          974 c          649 g         1225 t
ORIGIN      
        1 acttcgatct tcaagtttgc aggatcgact acaaaggcca aggtttccca aacatcttct
       61 tcactactag ctgttgtcaa tctcagtttg aagcgctctt tctcacttgc cggcgctgaa
      121 ttttcaagat cacccaagcg cctcaaatta gccaataaat tttcaactgc cacatcaccc
      181 attccagcac tggaaaactc tatggaatat gtcagtttcg gcaaatcatt ttgtggcgcc
      241 gcttcgttga taatcacatt tgaaggctta tggctagcag cttctactag catagcttct
      301 tctttagtac cagaatcctc aactacctcg gaaatcactg gagcttgtgt ttcatcactc
      361 aatcttttca gttcttcgca aacctctgcc gcaaccgtgg ggtctgcttc cccctctcct
      421 cgatgacctg ccagttgtgc ttttatgaca tcacctgctc tcaaaaaagc atccaccatt
      481 tcactacgta ctgcaagctc ccctttgcgc aatctatcca gcaatgtctc caacatatgc
      541 gtcatctccg tcatatctgt aaaaccaaaa gtgccagcac ctcctttgat agaatgtgca
      601 gcacggaaaa tagcgttcaa atcttccaaa tccggcgaat ttacatcaag cctcagcaag
      661 catgcttcca tatccgcaag taattctgag gattcttcaa aaaatatttc ataaaactgt
      721 tcgagatctg tactcataga taacctgttt tacgttgagt tgttaggcta acaccttaaa
      781 gaaataagca aatagaaaaa tagaataaat acattctaaa tcttattaaa aaagttccta
      841 tttgcaacct attaattcat ttggagacaa gtatttaacc aataacttta ccgattactt
      901 ctagcaacct tttcggatca aatggcttta ctagccagcc agtagcgcct gccgctctac
      961 cttttgcttt aatctcatgt ccggattcag tcgtcagtat caaaatagga atatttttat
     1021 agtgcgccaa tttacgtaac agttcgagca atttcagccc atccattcgt ggcatattaa
     1081 tatcagtcag cactaaatta acctgatgat aattagcttt atctaatgca tcttgaccat
     1141 ctactgcttg aatgacttca tacccagctc ccttgagcgt aaaagtaacc atctgcctga
     1201 tggaagcaga atcatccact gcaagtatcg tcttagccat ttatctcact cctattgcca
     1261 atatgttaat ttgtttgata aagcacgcga aagcgtgcta aaacagctca atatcaccca
     1321 tctgcatacc ttgctgcaca accggttttc ttttactttt agtttttgcc atattcactg
     1381 cttttgagac atcttcatgt attgtgtcta gcgtgttctt accaacattt tctctccaac
     1441 cccgctgccg ttctatgcgt tgcagttcaa tatttagcgc ttctacttga cgggaagtgt
     1501 gcgctagcaa ttgcgttacg agatcaccaa actgcaacga agttaccacc atttcgagtt
     1561 cctgctcgat tttgtctgca ataaccattt gtttctcaag caactccagt ataggtttac
     1621 tcccttctgg agccgccatt ttctcaatcg ccagaaccat ttcatgatgt gatttggtca
     1681 gcttgctgat atatccaaag ttaataacca aattattgac cgcatcgctt acaagccgat
     1741 caacctggtt taattctcct ttgatactca taaagtattc atcggcttca gcattacata
     1801 acacaccatc ttgctcaatc atcgacgatt gatgtgttac ttgattatca gattcgccac
     1861 cagctgtttg catctcagcc ataactttct ccttgtcgct cgtcttgatc gaaacaatta
     1921 aactgccatt cgacctagca aacctgctac ttatttttct ctgattgctt atatcctttc
     1981 gccaagaaag ctataggagt tgtcttgatg agcacgtacc agagcttctt atttcatcat
     2041 aaatcctttc atactcaatt agttaacggc acaacaatca ataactttag cacctaaaaa
     2101 taaaaaagct acgccatgta ctacatataa ctcagtaatc agtacttata cttcgatata
     2161 aaactaatta atattaatta gctgcttcac cgatccgatt agcccatgca atggcttgta
     2221 tttcaatatc ggctaattcg cttaaactta aaaaactcaa ctcagtcaga atcaattgaa
     2281 tcgcagcaac ttcacctttc tcgttggctt cgattagtct caattcctga cctaagcgac
     2341 cttcccgggt caacaaagct tggctcatgt catcaggaat tcccagttta tcaacgattt
     2401 gctgcatctc aatgccgagc agtacatcta ataaagataa tattcccacc atgaatgctc
     2461 tttcctggtg gtttttatcg tgaggacgct cagccagcgc gactaactcc atcatttttc
     2521 ctcgtgctgc cgcggtttgc attaatgcat tcgacatact atcgtcggat tgattggacg
     2581 tataaagcaa cagctgtatc catcgctgca gttgcttacg ccctaagatc atgatggcat
     2641 gcttaattga attgattttc tgcggtaacc cgctggcaac agaatttacc atacgcatca
     2701 agttgtagct tagcccgggc tgatgtttaa attcccgctc aagctcctcg atatcgctat
     2761 cccccataac caatgtcaat agcttcaaca gtgaaagctt ccctggatcg gcgcgtttta
     2821 tcgacaatac ttcaggtctt gcaaaataat atccttggaa catctgaaaa cccagctgca
     2881 tgcagtactt ctcttgttcc cggttctcca ctttcagtgc taggagtaat accggccagc
     2941 ggtttagttc agcaactaac ttcgcgagcg cattttgttc cagcgccaga acatccactt
     3001 tcaccacact cacgagtggc agtaactgtt ctaccttgct attcatctca acaatattat
     3061 ccagcgcaaa ctgatagcct ttttgcttta actcattaca gcactgcata aactctggtg
     3121 ttaccgttgc tgtttccttt atttccaata cgacatgctt gctcggcaac agactgatga
     3181 tgtcactcat taccaattca ggatttgcat tgataaatcc gcgctgctta cctagcacat
     3241 tttggattcc taactgacaa taagcgttga taatgacatt cgctgatgcg gataaatcat
     3301 ttttaatatt gatcgactct tcggtttctt cttgtctaaa cagcaactca aacgccacaa
     3361 ggttttgatt acgatctaga attggttgtc gcccaagaaa aaaaccttcc atttattccc
     3421 tcgtgttaat tcatcttttt aaacaaaagt tttaactcat cggctacatt gcgcgtttct
     3481 taagtatttt ttgttactca taaacactat gactcagagc tcttgcttga tcacagcaga
     3541 gttctcactc gtgaaaattt ttgattggat cagttagatc aatgcaggaa tagcacgatt
     3601 ctttccagta cgttttgctt gataaagtgc tttgtccgcc cggcttatga cggagttttg
     3661 atgttctcca aaagagcgca aggcaacccc cgcgctaaag gttattggca atgcttgact
     3721 gtctgattgg atcgagaaat tttttgctaa attgttttgt atccgttgaa taatcgtcag
     3781 tgcatcttgc atctcaacat caggcaacaa gatgacaaat tcttctcccc caaaccgcgc
     3841 cacaatatcg gagggccgta gcgtctcttt ggcaaccgtc accaaattga tcagtacact
     3901 atcaccgtat tgatg
//
LOCUS       DAOQLJ010000190         3908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_11713_length_3908_cov_5.916429, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000190 DAOQLJ010000000
VERSION     DAOQLJ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11713_length_3908_cov_5.916429"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PK522_10560"
     CDS             complement(<1..202)
                     /locus_tag="PK522_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HNC42215.1"
                     /translation="MSHHPRKRFGQNFLIDNHLIAAIIHVINPISSDRLVEIGPGMGA
                     LTKPLVKILDHLDVVEIDRDIVQ"
     gene            complement(214..1203)
                     /gene="pdxA"
                     /locus_tag="PK522_10565"
     CDS             complement(214..1203)
                     /gene="pdxA"
                     /locus_tag="PK522_10565"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNC42216.1"
                     /translation="MLTLALTAGEPAGIGPDLCIQWAQQHSLPYRVVVIADREMLEQR
                     AHRLGCSLTFVPYIASEWKPQQAGTLEIYPVPVQHPVDPGVLDPANADYVLETLKIAV
                     SGCQLQQFHAMVTAPVHKGVINGAGIPFTGHTEFLADLTQSQVVMMLVGGGMRVALAT
                     THLPLNAVADAITASTLEQRLRIIDRDLRTRFWIEHPCIAVAGLNPHAGESGHLGREE
                     IEIIIPLLTKLRAEGMNLIGPLPADTLFNPVKLQGYDCVLAMYHDQGLPVLKYASFGS
                     GVNVTLGLPLIRTSVDHGTALDLAGTGQADIGSFHQAIMMASTLAEKAARFQQ"
     gene            complement(1213..2565)
                     /locus_tag="PK522_10570"
     CDS             complement(1213..2565)
                     /locus_tag="PK522_10570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC42217.1"
                     /translation="MKKLFFVLISLTISIVFTSFLMSHDIAAQEEPVLTSLPVTRSVQ
                     SIDHIVAIVNEEVITRNELDEVIRTTVKQLQKQGVQLPPFDIIEKQLLERTIMNRAQL
                     QRAKEVGIAVTDTELDQTIRKIAKENNLSMDEFYYALQQDGMTLPEFREEIQNELLIS
                     RLKEREVNNRVTVTEGEIDNFLRTQETSAIGNDEYRVAHILVQILEQMDSAQIEARQQ
                     KAENALKRLQEGVDFAQISAELSDAPNALQGGEIDWRPITQMGPMFSDILIKMQPGDL
                     TPVIRSPIGFHILKLLGRRPQETPVVMVNQTHARHILIKINELTSEDDAHRVIAQIRE
                     RIAKGADFAETAKGMSEDASANNGGDLGWLSPGDTVPEFERAMEALMPGQMSQPVQTQ
                     FGWHLIQVMERRTQDVSLDRHRHSARQAIRNRKADMIMQEWLQQLRDQTYVEYRTEDS
                     "
     gene            complement(2611..>3908)
                     /locus_tag="PK522_10575"
     CDS             complement(2611..>3908)
                     /locus_tag="PK522_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HNC42218.1"
                     /translation="SRANLVQQAGLSYVGSLGHDGIINFNAMVQGFQTIQDPRAPIIS
                     PYKRLPQMTLNMSKRNVFGTDLDFTGVYTHFYRPDSQQVPESQSGHRMVMFPSISVPL
                     QTSYGYIKPKMGVHFTRYELGDSLKDVNVTDTNPQRVLPIFSLDSGVVLERDTKLGRT
                     NFVQTLEPRLYYLYIPFRNQNRLPNFDSAEMDFSFAQIFTENRFSGHDRINDANEVTF
                     AMTSRLIEQSTGNERVRIAAGQRVRFTERDVVLSNSQITSVKSDFIAAISGRITPVIT
                     TDSSIQLDQSELRTEKIRTGISYHPAPGKVLNVGYRFTRGIPGIERGNNLFPVPGAGF
                     EQVDFSTQWPVLKNWQAMASANYSLDDDKLLAGLVGLEYNACCWSLRFVMNRFVRATG
                     KESTTFFVQLELNGLMRIGTNPLAALRQGVPGYTRTSHQ"
BASE COUNT         1093 a          946 c          808 g         1061 t
ORIGIN      
        1 attgtacgat gtctcgatcg atttcaacga catcaagatg atcgagtatt tttactaaag
       61 gtttggtcaa tgcccccata ccaggtccaa tttctaccaa tcggtcacta cttattgggt
      121 tgatgacatg aataatcgct gcaatgaggt gattatcgat caaaaaattt tgaccaaatc
      181 gcttacgagg atgatgggac acagaaattt aggttattgt tggaaacgag ctgctttctc
      241 agcaagcgtg gatgccatca tgatcgcttg atggaaacta ccgatatcag cctgacccgt
      301 tccagctaaa tcaagtgcag tcccatgatc aactgatgtt cgaatcagtg gaagccccaa
      361 tgtgacattc acaccactcc caaaactggc atatttaagc acgggcaaac cttgatcatg
      421 atacatcgcc aacacacaat catagccttg caattttacg ggattaaata aggtatctgc
      481 cggtaagggg ccaatcagat tcattccttc agcgcgcaac tttgttaata gtggaatgat
      541 gatttcaatt tcttcacggc caagatggcc tgattctccc gcatgcggat tcagtcctgc
      601 gacagcaata caaggatgct caatccagaa acgggttcta agatcacgat caataatccg
      661 taatcgctgt tctagcgtgc tagccgtgat tgcatcggct actgcgttca gcggcaaatg
      721 cgtcgtagcc agcgcaactc gcatccctcc cccgaccagc atcataacca cttgactttg
      781 tgttaggtca gctagaaatt cagtatgccc cgtgaaggga attccagctc cattaatcac
      841 gcctttatga actggtgcgg tgaccatggc atggaattgc tgcaactgac aaccgctaac
      901 cgcaatttta agggtttcca acacataatc tgcattcgca gggtctagca caccaggatc
      961 aaccggatgc tgtactggca ccgggtaaat ttctaaagtc ccggcttgct gaggtttcca
     1021 ctctgaagca atataaggta caaaagttaa tgaacacccc aatcggtgcg cgcgctgttc
     1081 caacatttca cggtctgcaa tcacgaccac tcgataggga agcgaatgct gctgagccca
     1141 ctgtatacat aaatcagggc caataccggc aggttctcct gctgtcagcg ctaacgttaa
     1201 cattgactgc aattaactat cttctgtgcg atactcaaca taggtttgat ctcgtaattg
     1261 ctgtaaccat tcttgcatga tcatatctgc cttacgatta cgaatggctt gacgggcaga
     1321 atgacgatga cgatctaaac tcacatcctg ggtgcggcgc tccatcacct ggattaagtg
     1381 ccaaccaaac tgagtttgaa ccggttgact catctggcca ggcatcaatg cctccatcgc
     1441 cctctcaaat tcaggaacag tatcgcccgg cgatagccaa cctaaatcac caccattgtt
     1501 agcacttgca tcttccgaca tgcctttagc ggtttcagca aaatcagcgc cttttgcaat
     1561 gcgttctcta atttgtgcaa ttacccgatg cgcatcatct tctgaagtca attcattgat
     1621 tttaattaaa atatgtcgtg catgcgtttg attgaccatc acaactggtg tttcttgagg
     1681 cctacgcccg agaagtttca aaatatgaaa cccgattggg ctacggatga ccggtgtcag
     1741 atccccaggt tgcattttga tcaaaatatc agaaaacatc ggccccatct gggtgatagg
     1801 tcgccaatcg atttctccac cttgcaaggc attcggcgca tcagataact ctgccgatat
     1861 ttgggcaaaa tccactccct cctgtaagcg cttcaatgca ttctctgctt tctgttgccg
     1921 tgcttcgatt tgcgccgagt ccatttgttc caatatctga actaggatat gtgcgacacg
     1981 atactcatcg ttgccaatcg cagaagtttc ttgtgtccgt aaaaaattat cgatttcccc
     2041 ttcggtcaca gtgactcgat tattcacttc gcgttctttg agacgagaaa tcagcaattc
     2101 gttttgaatt tcttcacgaa actcggggag tgtcatgcca tcttgttgca aagcataata
     2161 aaactcatcc atggatagat tattttcttt agcaatctta cgaatcgtct ggtcgagctc
     2221 ggtatcggtg acagcaatcc cgacttcttt ggcgcgctgc aattgcgctc gattcatgat
     2281 ggtgcgctct aataactgtt tttcgataat atcaaacgga ggcaactgaa caccctgctt
     2341 ctgaagctgt ttgactgtcg tacggatgac ttcatcaagc tcattgcgcg taatgacttc
     2401 ttcattgaca atggcaacga tatgatcaat cgattgtacc gatcgtgtaa ccggcaagct
     2461 ggttaatact ggttcttctt gtgcagcaat gtcatggctc atcaaaaaag aagtaaaaac
     2521 aatactgata gttaagctga taagtacaaa aaatagtttt ttcattaaat tatccaacaa
     2581 catattgatt gactcctata ccaatctgat ttattgatga cttgtccggg tatagcccgg
     2641 cactccttgc cgcaacgcag caagcggatt agtcccgatt cgcatcaatc cattcaattc
     2701 aagctgcacg aagaaagttg tcgattcttt tccagtagct ctgacgaaac gattcattac
     2761 aaaacgcagc gaccaacaac atgcgttgta ttctaatcct actagtccag cgagtaactt
     2821 gtcatcatcg agtgagtaat tagcgctagc cattgcctgc caattcttta gaactggcca
     2881 ttgcgtagaa aaatcaactt gttcaaaacc agcaccgggc actggaaaga gattattccc
     2941 tcgctcgatc cccgggatac cccgtgtgaa acgataacca acattcaaca ccttaccggg
     3001 tgcagggtga tagctgatac ccgttcgaat tttttcagta cgcaattcac tttgatctag
     3061 ctgaatacta ctatctgtgg taataaccgg ggtaatacgc cccgaaatcg ccgcaatgaa
     3121 atctgattta acactggtaa tttgcgaatt ggacaatacg acatctcgct cagtaaagcg
     3181 aactcgttgt ccagcagcaa tacgcacacg ctcattcccc gttgactgtt caattaagcg
     3241 cgaagtcatg gcaaaggtaa cctcattggc atcattgata cggtcatgcc cactaaaacg
     3301 gttttctgta aagatttgcg caaagctaaa gtccatctcg gctgaatcaa aattaggcaa
     3361 tcgattctgg ttacgaaacg ggatataaag ataataaaga cgcggttcaa gcgtttgaac
     3421 aaaattggtt cgacccagct tagtatcgcg ttcaagcacg acaccgctat ccaaactaaa
     3481 aatagggagt acccgttgcg gattggtatc ggtcacattg acatccttta aggaatcacc
     3541 taattcatac cgagtaaaat gaacacccat cttaggttta atataaccat acgatgtttg
     3601 taagggaacg ctaatactcg gaaacatgac catacggtga ccgctctgcg attctggaac
     3661 ttgctgcgag tcaggtcgat aaaaatgcgt atataccccg gtaaaatcga gatcagtccc
     3721 aaacacattg cgtttcgaca tattcaatgt catttgcggt aaacgcttat agggcgaaat
     3781 aatcggtgca cgcggatctt gaatcgtttg gaaaccttga accatagcat tgaaattgat
     3841 tatgccatcg tgccctaatg aaccaacata ggataatcca gcttgttgga ccaagtttgc
     3901 tcgcgatg
//
LOCUS       DAOQLJ010000191         3897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_11787_length_3897_cov_6.921135, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000191 DAOQLJ010000000
VERSION     DAOQLJ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11787_length_3897_cov_6.921135"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1027
                     /locus_tag="PK522_10580"
     CDS             158..1027
                     /locus_tag="PK522_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HNC42219.1"
                     /translation="MKKILTLFTLAVLTFTLLPVDAEAKRLGGSKSIGKQRDSINQQA
                     TPKPAQSQSAAPASNPAAGGSKWGGALAGLAAGGLLAALFMGGAFDGINMADILMLVV
                     LMAVIFFIVRMLRKPKSEQLSRPMQYSAAGAAQTNEVFSGGSSATSSSSTDKAYDGNA
                     TTNSIPPDFQVEPFLKNAKLSFINLQAANDAGDVDAIREYTTPELFEALNKQIQESGD
                     HPQKTEVIFINADLLDVTTSSDMAIASVRFNGQLREAPDALPEPFDEIWHVQKSLKEK
                     NAPWLLAGIQQPD"
     gene            1160..1900
                     /locus_tag="PK522_10585"
     CDS             1160..1900
                     /locus_tag="PK522_10585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HNC42220.1"
                     /translation="MKIAVVSDIHGNLPALEAVVSHFNRIGIDKVINLGDSLSGPLLP
                     LETAHFLMAQNWIQLAGNHDHQILAHGTDQCSVVDELAYSQLSAKELDWLKSLPNTLY
                     LSQDILLCHGTPESSNSYLLETVVPSGARLATANEIKERLGKDRKEKLILCGHTHQPR
                     SVKILGELLIVNPGSVGLPAYSDEIPFLHTIETGTPDAHYALLERVGNQWISSLISVP
                     YDYQAMVKLAESRHFMDWACALRTGFMN"
     gene            complement(2215..3003)
                     /locus_tag="PK522_10590"
     CDS             complement(2215..3003)
                     /locus_tag="PK522_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNC42221.1"
                     /translation="MQIITSREHTLFKQLIKLLRSTQQRKREKLTILDGIHLVQTYYQ
                     VYGSPFKLIISESACQQPETESFLKQFNQSSEPNRSVVLSDTLFKHISPVVTPSGIMA
                     LITVPIASHIHSKQFCVLLDAIQDPGNLGSILRSAASAGVNAVYLSKTCADAWSPKTL
                     RAAMGAHFFLTIHENAELVQIAGNFQGKVFTTSACSEHSLFQTKLTEPIAFVFGNEGS
                     GISGELMDCVSQSLAIPMNKNCESLNVAAAAAICFFEKLRQNLN"
     gene            3314..3757
                     /locus_tag="PK522_10595"
     CDS             3314..3757
                     /locus_tag="PK522_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42222.1"
                     /translation="MNKQKYVIRQQDQNAAVEFLDKQISSGLYWLGEDASQGIVAERE
                     YQDAKANPNDLNAWCEKWLHETQWAQLKNAISIARDQIEKQKYRPQLKTVSLTHNAWE
                     ILSELAKNEGLTLSELIVNRLGNEWLNSYEQNTLPNHHFIKSRQM"
BASE COUNT         1252 a          657 c          819 g         1169 t
ORIGIN      
        1 tttattgagt tattttaaga gtatttcata aattggttag aaataaccaa cattaaaatt
       61 ctaattgcta ctagcattcc caaaaaattc caatgagaaa caagaataaa tcgatgatgt
      121 tatactttct aactcaacag tgtttatgga gaaaaaaatg aagaaaatac ttactttatt
      181 tacattagcg gtgcttactt ttactttatt gccagttgat gctgaagcga agcgcttagg
      241 cggtagtaaa agtattggaa agcagcgcga ttcaattaat cagcaagcaa cgcctaaacc
      301 tgctcagtca caatcagcag cacctgcttc taatccagcg gctggtggaa gtaagtgggg
      361 tggggcatta gcaggtttag ctgctggtgg gctactcgct gcattattta tgggtggtgc
      421 atttgatggc attaatatgg ctgatattct tatgctggtt gtattaatgg cagttatatt
      481 ctttattgta cgcatgctac gtaaaccaaa atctgagcag ctatcgcgtc ctatgcagta
      541 ttcagcagct ggcgcagcgc aaaccaatga agtattttct ggaggctcat cagcaacctc
      601 gtcaagctct accgataagg cttatgacgg taatgctaca actaattcga taccaccaga
      661 ttttcaggta gagccgttct taaaaaatgc aaaattatca tttatcaatt tacaagcagc
      721 aaatgatgcg ggtgatgttg acgctattcg tgaatataca actccggaat tatttgaagc
      781 attgaataag caaatacaag aaagtggtga tcacccacaa aaaacggaag tgatctttat
      841 taatgcggac ttgttggatg ttacgacgtc aagtgatatg gccattgcaa gcgtccgttt
      901 taacggtcaa ttaagagaag cacctgatgc attaccagaa ccttttgatg aaatctggca
      961 tgtacagaaa agccttaagg aaaagaacgc accttggtta ctcgccggta ttcaacaacc
     1021 tgattaatgt agtaaggaat cgataaatca attaccctgt ttcatttttt tgaaaggcga
     1081 ggcagggtaa ttcgattgta cttctatttt ctcgtgattc tagcaaagat aaatttcttt
     1141 tctatgtagt tgttatctaa tgaagattgc ggttgtttca gatattcacg gcaatttacc
     1201 cgcactagaa gcagtcgttt cacatttcaa tcgtataggt attgataagg taattaacct
     1261 tggcgatagc ttatctggtc ctttattacc actagaaaca gcacattttc tgatggccca
     1321 gaattggata cagcttgcag gtaatcatga tcatcagata ttagcgcatg ggactgatca
     1381 atgcagtgtt gtagatgaat tagcctattc tcagctttca gctaaagaat tagactggct
     1441 gaagagttta cccaatacac tgtacttgag tcaggatatt ttactttgcc acggtacacc
     1501 tgagagtagt aatagttacc ttttggagac tgttgtgccg agtggagcgc gcctagcgac
     1561 tgcaaatgaa attaaagaac ggctaggaaa agatagaaaa gaaaaattga ttttatgtgg
     1621 gcatacgcat cagccacgtt cggtaaaaat tttgggtgaa ttactcattg ttaacccagg
     1681 gagtgttggg ttacctgctt atagtgatga gataccgttt cttcatacga ttgaaacagg
     1741 tacccctgat gcacattatg cgttactaga aagagtagga aatcaatgga tatcttcctt
     1801 aatatcggtt ccttatgatt atcaagctat ggtaaaactg gctgaatcac gccactttat
     1861 ggactgggcg tgtgcattaa gaactggttt catgaattaa gaataaattt ctcagtagta
     1921 gaatattgcc attactagaa aatatgcgta gaatcctagc cagccctatt gtggttatgg
     1981 gtttataaaa ctaaagaata gattttcata aggagataag tgttatggaa aataatagag
     2041 cgacagaagc tagtacgttc aaagtagtag ctatttctac gattgggttg atcgttattg
     2101 gtgcagttgt ttattttgta actgcagtct aatagtggcg ttacgttagt tcaattggtt
     2161 tacactcata ctcagtaatg agtatgagtg gttgtaatat caaatagtcg tgcatcaatt
     2221 taagttttgg cgaagtttct caaaaaagca tatagcagca gcggcagcaa cattcaaaga
     2281 ttcacagttc ttattcatcg ggatagcgag tgattgacta acgcaatcca ttaattctcc
     2341 tgaaatacca gatccttcat taccaaatac aaaagcaatc ggttcagtaa gttttgtttg
     2401 gaacaagcta tgttcagaac acgctgaagt ggtaaatact tttccttgaa aattgcccgc
     2461 tatctgaact aactcagcat tctcatgaat ggttaagaag aaatgagccc ccattgccgc
     2521 acgtaatgtt ttgggagacc aggcgtctgc acaagttttt gataaataaa ccgcatttac
     2581 accagcgctt gctgcagaac gcagtattga tcctaagttt cctggatctt gtattgcatc
     2641 aagtaataca cagaattgct tggaatgaat gtggctagct atcggtactg taataagagc
     2701 cataatccct gagggagtta caacagggga tatatgtttg aataaggtat cgctcaaaac
     2761 aacagagcga ttaggctcag atgattggtt aaattgcttt agaaaagatt ctgtttcagg
     2821 ttgttgacaa gcagattcac tgataatgag cttgaatggt gatccataaa cctgatagta
     2881 agtttgaaca agatgtattc catccaaaat ggtaagtttt tctcgctttc gttgttgagt
     2941 cgaacgcagt agctttatta gctgtttgaa taaagtgtgt tcacgggaag tgatgatctg
     3001 cataaaagat aatttgtaag atgtatgaat ttaagcgttg caatgtattt aggaaaaaac
     3061 tgtgattgga tttgggtgat aaaaggtaac acagtaggca cctcaaagtc ggctgattac
     3121 gatttcaatt tttaactcca ggattacttc aaataagact atataaacta gcagcttaaa
     3181 cacgatagta atattgtttc aagtgtttag tgcgtaaatc tgaataaaga cttgcctgaa
     3241 taatgaattg gcttgtatta tctactcata aaaaatctta gtagttttat gaaactaatg
     3301 agagataata aaaatgaaca aacaaaaata tgtaattaga cagcaagacc agaatgctgc
     3361 agttgaattc ctcgataaac aaatctcatc aggtttatac tggctaggcg aagatgcgtc
     3421 tcaaggtatc gtcgctgaac gtgagtatca ggatgccaaa gccaatccta atgatttaaa
     3481 tgcatggtgt gaaaagtggt tgcatgaaac tcagtgggca caacttaaga atgccatcag
     3541 tattgcaagg gaccaaatag aaaagcagaa atataggcct caacttaaaa ctgtgagcct
     3601 aacccataat gcttgggaaa tactttcaga acttgcaaaa aatgagggat taactttgtc
     3661 agaattgatt gtcaatcggc ttggtaatga gtggctgaat tcttacgaac aaaacacttt
     3721 acccaatcat cactttatca agagtcgcca aatgtgagtg ataacctgtt aatcctgaat
     3781 ttgttggact aggttagatg tgatttaata atctggtcga gggagtttcc ccctctttct
     3841 cagataaaat taaataaaag aaagaggaac tgaataagag attgaccatt aatgaag
//
LOCUS       DAOQLJ010000192         3883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_11878_length_3883_cov_4.397858, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000192 DAOQLJ010000000
VERSION     DAOQLJ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11878_length_3883_cov_4.397858"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1644)
                     /locus_tag="PK522_10600"
     CDS             complement(22..1644)
                     /locus_tag="PK522_10600"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HNC42223.1"
                     /translation="MKQLYGKSLLDDPTLNKSTAFTREERKRFGLSGLLPYRVTDIYT
                     QTARVLNNMRSKHSAIEKYIFLNSLLERNQCLFYRTLIDHIEEILPLVYTPTVGEACK
                     QFGHIFRKPQGFYITPEDKGEIASILGNWPETDIRIIVVTDGERILGLGDLGANGMGI
                     SIGKTALYVACAGLHPENCLPVMLDVGTNNQALREDPFYMGYPHPRITGDEYLSLVDE
                     FVQAVQTRFPDAVIQFEDFHNQHAFQLLDRYRESVRCFNDDIQGTAAVTLSGIYTSCH
                     ITQKRFTDLNIMLLGTGSAASGIAGLLVSAFCAAGLTEAEARSRLWFIDRQGLVTSNR
                     EEIKPLIKPFAHPCAPATFLEAINRIRPDVLIGATGMAGTFNQDIITAMATVNERPVI
                     IALSNPTSHTECTAEEAYRWSNGRAIFASGSPFEPVTYAGKLLKPGQGNNAYIFPGVG
                     LGVTISKARLVTEPMFLAAAHALSAQILPEEIAAGSIYPSINRVRPVSLAIAVAVCQV
                     AKQAGLTEETLPETDLESYIAAKMYDPRYRVG"
     gene            1896..2321
                     /locus_tag="PK522_10605"
     CDS             1896..2321
                     /locus_tag="PK522_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HNC42224.1"
                     /translation="MTTLQSIPSPNPDLYTEEEISNLVHQFYAKARKDPDLGPIFEGH
                     VIDWDAHFVQMTNFWSAQLRGTNRFRGAPMPKHIALPALNADLFKRWLALFKETTQEL
                     GNPTLKHHADMVATFIAGRLWLGYQMSNYPDQPATSLAV"
     gene            2412..3353
                     /locus_tag="PK522_10610"
     CDS             2412..3353
                     /locus_tag="PK522_10610"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNC42225.1"
                     /translation="MQVFRSTDTPHKIPLALTIGNFDGVHLGHQAMLEELKQAAKRLN
                     IATCIMIFEPHPREFLMPGQSPTRLTSLREKLEIFAQLDIDRVQICHFNHAFAQISAQ
                     TFITDILMQQLNVRWMLVGDDFRFGAKRVGDVAMLKAYAQQTGQFNVEVMADFSINHI
                     RVSSTAIREALSAGNMALAQQLLGRPYSMSGRIVNGDKLAKQLGFPTANIHFKHSHPP
                     LRGIFVVEVISRENSNLPKVMQGVASLGVRPTTHASDKLILEVHLFDFDQEIYGQHLQ
                     VNFLHKLRDEEKYADLEKLIAQIKQDIKNAKHYFMLH"
     gene            3387..>3883
                     /locus_tag="PK522_10615"
     CDS             3387..>3883
                     /locus_tag="PK522_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNC42226.1"
                     /translation="MTDYKNTLNLPDTPFPMRGDLAKREPGMLKAWQEKKLYQKIREI
                     SQGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIKSKTLAGFDAPYVPGWDCHGLPI
                     EHQIEKKHGKHLPADQVRTLCRAFAQAQIERQKADFIRLGVLGDWDNPYLTMNYQTEA
                     DIIRAL"
BASE COUNT         1073 a          869 c          889 g         1052 t
ORIGIN      
        1 tgagtgaccg agcggcaaat cctaacctac cctataccgt gggtcataca tttttgcagc
       61 gatgtaactt tctaaatccg tttcaggtag cgtttcttct gttaaacccg cttgttttgc
      121 gacttggcaa actgcaactg caatcgcgag tgaaacgggg cgcacgcgat tgatcgaagg
      181 gtaaatcgat ccggctgcga tttcttccgg taaaatctgc gctgagagtg cgtgggcggc
      241 agctaagaac atcggttctg tcactaatcg tgctttgctg atggttaccc caaggccaac
      301 ccctggaaaa atgtaagcat tgttgccttg acccggcttg agcagttttc ctgcgtatgt
      361 caccggttcg aatgggctcc cactggcaaa aatcgctcta ccattggacc atcgataggc
      421 ttcttccgca gtgcattcag tatgagacgt tgggtttgac agcgcaatga tcacgggacg
      481 ttcgttgacg gttgccatcg cagtgatgat gtcttgattg aatgtcccag ccatgcccgt
      541 ggcgccgatg agcacatcag gacgaattcg gtttatcgcc tccagaaagg tcgcgggagc
      601 acagggatgt gcaaagggtt tgataagcgg tttgatttct tctcgattcg atgtaactaa
      661 cccttgccga tcaataaacc ataacctgga tcgtgcttct gcttcagtca gtcccgctgc
      721 gcaaaaagcg gacaccagca accctgcaat tccgctcgct gcagatccgg tgccgagcaa
      781 catgatattc aaatcggtaa atcgcttctg cgtaatgtgg catgaagtat aaattcccga
      841 gagcgtgacg gcggcagtgc cctggatatc atcgttaaag caacgcacag actcacgata
      901 gcgatccaat agttggaagg catgctggtt atggaaatct tcaaattgaa tcaccgcatc
      961 agggaatctg gtctgcaccg cttgcacaaa ctcatcgacc aaggataaat attcatcccc
     1021 agtaatacga gggtgtggat atcccatata aaaaggatct tcacgcaagg cttgattatt
     1081 ggttccaaca tcgagcataa cgggcaaaca gttttcggga tgaaggcccg cacatgcgac
     1141 atacaacgct gttttaccaa tggaaattcc catcccattg gcacctagat cgccaagccc
     1201 gaggattcgt tcaccatcgg taacgacaat gatgcgaata tcggtttcag gccagttacc
     1261 gagtatcgat gcgatttctc ccttgtcttc gggagtgata taaaaccctt ggggttttct
     1321 gaaaatatga ccaaactgct tgcaagcttc tccaacggta ggtgtgtaaa cgagcggtag
     1381 gatttcttca atgtgatcga tcagggttcg gtaaaacaaa cattgattgc gctcaagtaa
     1441 gctgttcaaa aaaatatatt tttcgatggc gctatgcttg cttcgcatat tattgagtac
     1501 gcgcgcggtt tgggtataga tgtcggtaac acggtaaggt aataagccgc ttaacccgaa
     1561 gcgcttccgt tcttcgcggg taaatgcagt tgatttattg agggttggat catctaacaa
     1621 ggattttccg tacagttgct tcatcctttt ctctccgcat aaaatgggct gcaaatgcaa
     1681 attgcgaaat ggagtgcgtg gggattaacc atccatccaa tacgctagtc tgtcgttaga
     1741 caattttgat agtaatcgat aaactagcag gcaaaggaaa caattaaacg attcacgtat
     1801 cgtcacatat ctagtgcgat aagtcgaggt tttccaaaat gactcagcaa tcccattaac
     1861 cgatttatta tttgataact gttttttttc tttatatgac gacactgcaa tcgatacctt
     1921 ctcctaatcc tgatctctat actgaggaag agatttcaaa ccttgttcat cagttttatg
     1981 ctaaagcaag aaaggatcct gatttaggtc ctatctttga aggtcatgtc atcgattggg
     2041 atgcgcattt tgttcaaatg acaaactttt ggtcagcaca attgagggga accaatcgtt
     2101 tccggggtgc tcccatgcca aaacatattg cacttcctgc actgaatgcg gatttgttca
     2161 aacgctggtt agcgttgttc aaagaaacga cgcaagaact cggcaaccca accttgaaac
     2221 atcatgcgga tatggttgct acctttatcg caggtcggct atggttaggc taccaaatga
     2281 gtaattatcc cgatcaaccc gcgacatcgc tcgctgttta gcaggcagct agaaattgtg
     2341 tctaataggg ggctagatag cgaccagtct agggagaaac gaaaagcgct ggctttatct
     2401 tagcagttac catgcaagtt tttcgtagta ctgacacacc gcataaaatt ccacttgcat
     2461 tgacgattgg taattttgat ggcgtccatt taggtcacca agccatgttg gaggaactca
     2521 agcaagctgc aaagcggttg aatattgcaa cttgcattat gatttttgag ccgcatccgc
     2581 gtgaatttct aatgcccggt caatcgccga ctcgattgac cagcttgcgt gaaaagttgg
     2641 aaatttttgc gcaattagat attgatcgcg tccaaatttg ccattttaac catgcatttg
     2701 cgcaaatctc ggcgcaaaca tttattactg acatattaat gcagcaatta aatgttcgct
     2761 ggatgttagt cggcgatgat tttcgatttg gtgctaaacg cgttggtgat gttgccatgc
     2821 taaaggccta tgcgcagcaa acgggacagt tcaatgtaga agtcatggcc gatttctcga
     2881 ttaatcatat ccgagtttcc agtactgcga ttagagaagc cttatcagct gggaacatgg
     2941 cacttgccca gcaattattg ggccgaccct atagcatgag cggtcgcatc gtcaatggag
     3001 acaaattagc gaagcagctt ggttttccaa ccgcgaatat ccacttcaaa catagtcatc
     3061 cacccttgcg cggtatcttt gtagtagaag tcattagtcg agaaaattcg aacttgccca
     3121 aagtgatgca gggcgtggca agcttgggtg tcaggccaac gacgcatgct tctgataagt
     3181 taattttaga agttcacttg tttgattttg atcaagaaat ttacggtcaa catttacaag
     3241 tcaatttttt gcataagctc cgagatgaag aaaaatatgc agacctcgaa aaattgattg
     3301 cacaaatcaa acaagacatt aagaatgcaa aacactattt tatgcttcac taacctttgc
     3361 cttgcactaa tatagagctt catttcatga ctgattacaa aaacacactg aatctacccg
     3421 acacaccctt cccgatgcga ggcgatctgg ctaaacgtga gccagggatg ctcaaagcct
     3481 ggcaagaaaa aaagctttat cagaaaattc gtgaaattag ccaaggaaga ccaaagttta
     3541 ttctgcatga tgggccaccc tatgcaaatg gtgatattca tattggtcat gcggtcaata
     3601 aaattctcaa agatatcatt attaaaagca aaaccttagc cggttttgat gcaccctatg
     3661 ttccaggctg ggattgccac gggttgccga tcgaacatca aattgagaag aagcacggca
     3721 aacatttacc tgccgatcaa gtccgcacgc tgtgccgagc gtttgcgcaa gcccaaatag
     3781 aaaggcaaaa agccgatttt atccgcttag gtgtcctggg tgattgggac aatccttact
     3841 tgacgatgaa ttatcagact gaggctgata ttattcgggc gct
//
LOCUS       DAOQLJ010000193         3831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_12154_length_3831_cov_6.037871, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000193 DAOQLJ010000000
VERSION     DAOQLJ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12154_length_3831_cov_6.037871"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PK522_10620"
     CDS             <1..235
                     /locus_tag="PK522_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HNC42227.1"
                     /translation="SGIIDLDVDLVQLYLDDVLVAEQGGRAKSYQEEAGQRVMKQAEI
                     TVRVVLNRGIMTATVWTCDFSYDYVKINADYRS"
     gene            242..1138
                     /locus_tag="PK522_10625"
     CDS             242..1138
                     /locus_tag="PK522_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC42228.1"
                     /translation="MSQFDLVLDRLNDLLIRVEALLPNQQDMTNFDAATAFRWRKQGH
                     NGYLQPVTHPHQIQLDALQGIDEQKQLIVQNTYQFVLGYPANNVLLTGARGTGKSSLV
                     KALLHQYADKGLRLIEVEKQDLIDLPDITEQIYQRPERFILYCDDLSFEMHEPGYKAL
                     KAILDGSIARASENILVYATSNRRHLMPESMKDNLDTRHIDGDIHPSESVEEKISLSE
                     RFGLWLSFYPFDQTQYLDIVRYWLAHYGIQHMNDAVRLEALRWALARGGRSGRIAWQF
                     ARDWSGKRKLAQSLRRKTRQSS"
     gene            1149..2093
                     /locus_tag="PK522_10630"
     CDS             1149..2093
                     /locus_tag="PK522_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HNC42229.1"
                     /translation="MIEVAAAVLVRPDGHFLLTSRPAGKPYSGYWEFPGGKIEKDESV
                     LQALARELYEELGIQIEQASPWITKIYAYQHATVRLHFYRVFTWHGELIGRENQQLSW
                     QTPDKVIVAPLLPANQPIIRSLSLPSVYGITQAAELGESTMLQKIELAAQQGLRLLQV
                     REKSMNKTQLKQFIDAIFKRTHPYQMKILINEDIALANAVGASGVHLTSSQLMDMSVR
                     PDLAWCGASCHNIEELYHAEQLELDFVVLGPVLPTKSHPDAITLGWRKFSSMIRDYPL
                     PVYALGGMIQADLPFAIEQGAHGIAMLRDIWKIEAVLK"
     gene            2107..2739
                     /locus_tag="PK522_10635"
     CDS             2107..2739
                     /locus_tag="PK522_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42230.1"
                     /translation="MKKRVWVIILILIGLTGCSTIYFLEGKEYEGRRQYIAARAEMYQ
                     RCIEGTISLNPPPIQRKLIALIPSQEAIFRTHFDNIKESNPNFPVTMEDLRQDPIYFG
                     MNENYRLVVELIKKRNLYQDLQIEEYETLTVPEVDEESDVFYIALPKDTKHRDMYYLN
                     TEKRGKLRVDYGPMDPDCETARAPFLALIPCEQFRDQLLTAIEILAKQSP"
     gene            2934..>3831
                     /locus_tag="PK522_10640"
     CDS             2934..>3831
                     /locus_tag="PK522_10640"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC42231.1"
                     /translation="MSKLIQAPVHRHEIFAWALFDFANSGYTTVVLTAIFNAYFVGVI
                     AQAYSSGTATLWWTITTAIANGVVLLTAPIIGAIADDRAWKKRFLMISASGCVGFTTL
                     LATAGPGDVSYAMALVILASICFFTGENLIAAFLPEIATPDKMGRISAFGWTIGYLGG
                     LLTLVLCLWVTLNTAAQGAEMADAVPLTLLIVAAMFVVGSLPTFIWLRERVQPCERTA
                     INYCSIAYQRLHTTWQHARFHRDLFQFLIALTIYTCGINTVIVLAAVYAQEAMGFTTE
                     DNIQLILIVNISAALGAFVFGQI"
BASE COUNT         1055 a          760 c          896 g         1120 t
ORIGIN      
        1 ctcaggaatc atcgatttag atgtagattt agtgcagctc tatctggatg atgtattggt
       61 ggcagaacag ggtggtagag ccaaaagtta tcaagaagag gcaggacagc gggtgatgaa
      121 acaagctgaa atcacggtac gggtcgtttt aaatcgaggc atcatgacag caacggtatg
      181 gacctgcgat ttttcttacg actacgtcaa gattaatgct gactatcgca gttgattgat
      241 gatgtcacaa ttcgatttgg ttcttgatcg gctcaatgat ttgctgatac gcgttgaagc
      301 attgctgcct aatcagcagg atatgacaaa ttttgacgca gcgacggctt ttcgctggcg
      361 caaacaaggt cataatggtt atctgcaacc ggtaacacac cctcatcaaa ttcagctcga
      421 tgcattgcag ggtatcgatg aacaaaagca attaatcgtt cagaatacct accagttcgt
      481 cttgggttat cctgctaaca atgtgttact gacgggtgca cgcggtacag gaaaatcttc
      541 cttagtaaaa gcattactcc atcaatatgc ggataaaggg ttacgcttaa tcgaggtaga
      601 aaagcaagat ctaatcgact tgcccgatat tactgagcaa atttatcaaa gaccagagcg
      661 ttttattcta tattgtgatg acttatcgtt tgaaatgcat gagccaggct ataaagcact
      721 caaagcgatt ttagatggtt cgattgctcg tgcatcggaa aatatcttgg tttacgcgac
      781 ctcaaatcga cgccatttga tgccagaatc gatgaaagat aatttggata cacgtcacat
      841 cgatggtgat attcatccga gtgagtccgt agaagaaaaa atttcgctat cagaacggtt
      901 tggtttatgg ctttcttttt atccttttga tcagacgcaa tatctcgaca tcgttcgcta
      961 ttggctagcg cattacggta ttcagcatat gaatgatgcc gttaggttag aggcattgcg
     1021 ttgggcatta gcaagaggcg gaagaagcgg gcgtattgcc tggcagtttg cgcgtgattg
     1081 gtctggtaaa cgaaaattag cacagtcact acgccgcaag actcgtcaat cctcataatt
     1141 ttgagtaaat gattgaagtt gctgctgctg tgttagttcg acccgatggg cattttttgc
     1201 taaccagccg accagcggga aaaccctatt caggttattg ggagtttcct ggtggaaaaa
     1261 ttgagaaaga tgaatctgtt ttacaagcgc tcgcacgtga actgtacgaa gagctaggaa
     1321 ttcaaattga gcaggcaagc ccttggataa caaaaattta tgcttaccag catgcaacag
     1381 taagactgca tttctaccgc gtgtttactt ggcatggtga gcttattggt cgtgagaatc
     1441 agcaattgtc ctggcaaacg cctgacaagg ttattgttgc accgctgctt cctgctaatc
     1501 agccgattat tcgtagccta tccttaccct ctgtttatgg aattactcaa gccgctgaat
     1561 tgggcgaatc aaccatgctg caaaaaattg aactcgcagc acaacaaggc ttaagactac
     1621 tgcaggttcg cgagaaatcg atgaataaga cacagctaaa acaatttatc gatgcgattt
     1681 ttaagcgtac tcacccgtat caaatgaaga ttttgataaa tgaggatata gcgctagcga
     1741 acgcggttgg agccagtggt gtgcatttaa cttctagcca attgatggat atgtctgtcc
     1801 gacccgactt ggcttggtgc ggggcttctt gtcacaacat cgaagagctt tatcacgctg
     1861 aacaacttga attagatttt gtggtgttgg gtccagtatt accgacaaag agtcatcccg
     1921 atgcgattac attaggctgg agaaaatttt caagtatgat tcgggattat ccccttccgg
     1981 tttatgcatt gggtggcatg atacaagcag atttaccttt cgcgattgaa cagggagctc
     2041 atggtattgc gatgttgcgg gatatatgga agatagaagc agtactgaaa taaggtgaag
     2101 attcttatga aaaaaagggt gtgggtcatt attctgatac tgatcggatt aactggttgc
     2161 agtacgattt atttccttga gggcaaagaa tatgaaggca gaagacaata tatcgctgct
     2221 cgtgctgaga tgtatcaacg ctgtattgaa ggtacgatat cccttaaccc tccaccgata
     2281 caacgaaaat tgatcgcatt gattccttca caggaagcga ttttcagaac tcatttcgat
     2341 aacatcaaag aatccaatcc caattttccg gttacgatgg aagatttgcg acaagatcct
     2401 atttattttg ggatgaatga gaattatcgg ttagtggttg agttgatcaa aaaacgaaat
     2461 ctctatcaag atttacaaat cgaagaatat gaaacactaa cagtacccga agtcgatgaa
     2521 gaaagtgatg tgttctatat tgccttaccc aaagatacca agcaccgaga tatgtattat
     2581 ctgaatactg aaaaaagggg taagctacgc gtggattatg gtccgatgga tcctgattgt
     2641 gaaacagcgc gcgcaccgtt cttagcattg attccttgtg aacaatttcg tgatcagtta
     2701 ctcactgcca ttgagatact cgcgaaacaa tcaccttaat cttggttagg ctggggttct
     2761 tctcagcatt gcgtcactcg catcaccgcg tcactaacaa tcgtattatt taagcagcta
     2821 aagtgtgtaa gttttatttg cagtaacact cggtaagtga tgctcagttc aatatttggc
     2881 ttatcgtatt actttaaatt ataaattcaa tggcctagaa gataaatcga acgatgtcca
     2941 aattaatcca agcaccggta caccgacacg aaatttttgc ttgggcacta tttgattttg
     3001 ccaattctgg ttataccacc gttgtattaa ccgcgatatt caatgcttat ttcgttggtg
     3061 tgattgcaca agcttattct tctggaactg cgacgctgtg gtggaccatt actacagcaa
     3121 ttgccaatgg tgttgtttta ttgaccgccc ccattatcgg ggcgatcgca gacgatcgtg
     3181 cttggaaaaa acgctttcta atgatttccg caagtggctg tgtaggcttt accacgttgc
     3241 tggcaaccgc aggtcctggt gatgtaagtt acgcaatggc tttagtgata ttggcaagta
     3301 tttgtttttt tacgggcgag aatcttattg ctgcttttct tccggaaatt gcaacgccag
     3361 ataaaatggg tcgaatttca gcgtttggat ggacaattgg ttatctcggt ggcttactga
     3421 ctttggtgtt gtgtctatgg gttaccttaa acactgcggc gcagggggca gaaatggccg
     3481 atgctgtgcc actgacattg ctgattgtgg cagcgatgtt cgtcgtgggg agcttaccta
     3541 ctttcatttg gttgcgcgaa cgtgtccaac cttgtgagcg aactgctatc aattattgct
     3601 cgattgctta ccaacgccta cacacgacat ggcagcacgc gcgttttcat cgggatttat
     3661 ttcaatttct cattgccttg acaatttata cctgtggaat taacacggtc atcgtgctgg
     3721 ccgctgttta tgcgcaagaa gcgatgggtt ttacgacaga agataatatc caactcattc
     3781 taatcgttaa tatcagtgct gcgctgggcg catttgtctt tgggcaaatt c
//
LOCUS       DAOQLJ010000194         3826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_12180_length_3826_cov_6.604879, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000194 DAOQLJ010000000
VERSION     DAOQLJ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12180_length_3826_cov_6.604879"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2332
                     /gene="ileS"
                     /locus_tag="PK522_10645"
     CDS             <1..2332
                     /gene="ileS"
                     /locus_tag="PK522_10645"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNC42232.1"
                     /translation="DIIRALGKIYQNGHLYQGQKPVNWCIDCGSALAEAEVDYENKTS
                     PAIDVGFEVVADESAAQTLTNIFSSAIPEGRKCYAVIWTTTPWTLPANQAVCVHPDFD
                     YELVDTPRGLLILAAQLKEACLTRYQINGETLARCKGSALEHLVLHHPFDTRTVKIIC
                     GKHVTLEAGTGLVHTAPAHGLDDYFIGQQYSLPSDSPVGNDGKFTQPTPLVGGMFVWK
                     ANDVVIESLQQSQHLIHSEKIEHSYPHCWRHKTPIIFRSTPQWFIGMNKPQGANMDQY
                     ESLRELAIKAVDQTTFYPAWGRARLEAMIKNRPDWCISRQRNWGVPMTFFVHKETQAV
                     HPRTLELLEAVAKAVEQQGIEAWFSLDPATLLGNEADQYQKLTDTLDVWFDSGTTHDT
                     VLRRNIHLNHPADLYLEGSDQHRGWFQSSLLTGCAMNHRAPYDALLTHGFVVDGQGHK
                     MSKSKGNVIAPQKIVDTLGADILRLWVASTDYSGELFISDEILKRIVETYRRIRNTLR
                     FLLANIADFNPQTDQLPVEQWLEIDRYMLAHTEALQNELTALYQRYEFHQSVHALHNF
                     CSEDLGGFYLDILKDRLYTTGSKNLPRRSAQNALYHITQSLVRLFAPILSFTAEEVWE
                     QLTNKPEDSVLLQSWHSFPVQARDQIDFLLARWSRLRQLRSQVLKKLEESRAQGAIGS
                     SLAAVVTIQAAGEDFTLLQSLGHDLRFVMITSEVHLHHIAHTNDIMITVSASSHQKCE
                     RCWHYREDVGHHADHPTLCQRCISNLYGAGEHRKHA"
     gene            2363..2827
                     /gene="lspA"
                     /locus_tag="PK522_10650"
     CDS             2363..2827
                     /gene="lspA"
                     /locus_tag="PK522_10650"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNC42233.1"
                     /translation="MKLYLSLIIAFIVVALDLVTKYWVESALVFGQQIPITSFFNLVL
                     TYNPGAAFSFLGDASGWQRWFLSGIAFIACAVISYLLYKHTENKLFCWALSLVLGGAL
                     GNLYDRITLGHVVDFLDFYIGDYHWPAFNVADSAICIGAALLIYESFTTKKP"
     gene            2904..3221
                     /locus_tag="PK522_10655"
     CDS             2904..3221
                     /locus_tag="PK522_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNC42234.1"
                     /translation="MKKAYSANNLIEAQMMLQLLEQAYIPAKLMNTFSQGGLGEIPFT
                     HAYPEVWVIRDDDFEKAQNIIDQYEHTSIDPGHVNCPNCSESNPANFELCWNCGHGLE
                     NKG"
     gene            3425..>3826
                     /locus_tag="PK522_10660"
     CDS             3425..>3826
                     /locus_tag="PK522_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNC42235.1"
                     /translation="MFLQNLASAGSFLVMLTIISCGFANIATASNTSTPAGKASMDPL
                     MDLSIEELIQIKITSVSRREQKLSGTPSAIFVITQEDIRRSGATSIPEALRMAPGVEV
                     ARVGTDKWSISVRGFNGRFANKLQVLMDGRSV"
BASE COUNT         1035 a          835 c          855 g         1101 t
ORIGIN      
        1 tgatattatt cgggcgctag gtaaaatcta ccaaaacggc catctctatc aaggtcagaa
       61 acccgtcaat tggtgtatcg attgtgggtc agcgctcgct gaggcagaag tcgattacga
      121 aaacaaaaca tcccctgcaa ttgatgtggg atttgaggtt gttgctgatg aatctgcagc
      181 acagacgcta acgaatattt tctcaagcgc tattccggaa ggtagaaagt gttatgccgt
      241 catttggacc actacaccct ggacgttgcc agccaatcaa gcggtttgcg tgcatcctga
      301 ttttgattat gagttagtcg atacaccgcg cggattgctg attttagcag cacagctaaa
      361 agaagcatgt ttgacgcgat accaaatcaa cggggaaaca ttggcccgtt gcaaaggcag
      421 tgcgcttgag catttggttt tgcatcaccc ttttgatacg cgaacggtga aaattatctg
      481 cggtaaacat gtaacgctgg aagccggtac cggtttggtt cataccgcac ctgctcatgg
      541 tttggatgac tatttcatcg gacagcaata cagtttaccc agtgatagtc ccgtggggaa
      601 tgatggtaaa ttcactcaac cgactccatt ggtcggcggt atgtttgttt ggaaagctaa
      661 tgatgtcgtg attgagtcgc tccaacaaag ccaacatctc attcattctg agaaaattga
      721 acatagctac ccccattgtt ggcgccacaa aacaccgatt attttccgtt caaccccgca
      781 atggtttatc ggtatgaata agccgcaggg tgcgaatatg gaccaatatg aatcactgcg
      841 tgaattagcg attaaagctg tcgatcaaac gacattttac ccagcctggg gaagagcacg
      901 cttagaagcg atgatcaaga atcgccccga ttggtgcatt tcacgtcagc gaaattgggg
      961 cgttccgatg actttctttg tccacaagga aactcaagcc gtacacccac gcacccttga
     1021 gttactcgaa gccgtcgcca aagccgtaga acagcaaggc attgaagctt ggtttagcct
     1081 cgatcctgct acgttgttag gcaatgaagc ggatcagtat caaaaattaa ctgatacgct
     1141 cgatgtttgg tttgattcag gtacaaccca tgatacggtg ctgagacgta atattcactt
     1201 gaatcatcca gccgatctct atttagaagg ctctgatcaa catcgtggct ggttccaatc
     1261 gtcactttta actggctgtg caatgaatca tcgcgctcct tacgatgcgt tactcaccca
     1321 tggttttgtg gttgacggtc aaggccacaa aatgagcaaa tccaagggta atgtcattgc
     1381 accccaaaaa attgtcgaca cgctaggcgc tgatatcctg cgtttatggg tcgcttctac
     1441 cgattattcg ggtgagttgt ttatctctga tgagatttta aaacggatcg tcgaaaccta
     1501 tcgccggatt cgtaataccc tacgtttttt actcgctaac atcgcagatt tcaatcctca
     1561 aaccgatcag ctaccggttg aacaatggtt ggaaatcgat cgttatatgc tcgcgcatac
     1621 cgaagcgttg caaaatgagc tgacggcgct ctatcaacgt tatgaatttc atcaaagcgt
     1681 gcatgcttta cataacttct gctcggagga cttaggcggt ttttacttgg atattttgaa
     1741 agaccgttta tacactaccg gttcgaagaa tcttccgcgg cgttcggcgc aaaatgcgct
     1801 ttaccatatc acacagagtc tggtacggtt gtttgcaccg atattgagct ttactgccga
     1861 agaagtctgg gaacaattga caaataagcc tgaggatagt gtgttgctac agtcgtggca
     1921 ttcatttcct gtgcaagcac gtgatcaaat cgatttttta ttggcacgct ggagccgctt
     1981 gcgtcaattg cgttcacaag tattaaaaaa attagaggaa tcgcgcgcgc agggagcaat
     2041 cggttcctcg cttgcggccg ttgtcacgat tcaagcagcg ggagaagatt ttacgctatt
     2101 acaatcgcta ggccacgatc tgcgttttgt catgatcacg tctgaagttc atttacatca
     2161 catagcgcat accaacgata tcatgattac agtttcagca agctcgcatc aaaaatgtga
     2221 gcgctgttgg cattatcgtg aagatgtcgg tcatcatgcc gatcatccaa cgctctgtca
     2281 gcgttgtatc tccaatttat atggcgctgg tgaacatcgt aaacatgcct aaatttcttg
     2341 tattgattac ctactcatta tcatgaaact ctatcttagt ctgatcatcg ctttcattgt
     2401 tgtagcactc gatttggtta caaagtattg ggtagagtcg gcgttggttt ttgggcaaca
     2461 aattcccatc acttcctttt ttaatttggt gctgacatat aatccaggcg ctgcatttag
     2521 cttcctgggc gatgcctccg gctggcaacg gtggttttta agtggcatcg cttttatcgc
     2581 atgcgcagtc atcagttatt tactttataa acacactgaa aataaattgt tttgctgggc
     2641 gttgagtttg gtactcggtg gtgcgctagg aaatttatat gatcgcatta cattaggcca
     2701 tgtcgttgat tttctcgatt tctatattgg cgactatcat tggcctgctt tcaacgtggc
     2761 tgactcggca atttgtattg gcgcagcatt gctaatctat gaaagtttta caaccaagaa
     2821 accctaaatg aatcgtgtat taattgcggt accgctgtga tgtattcagg aaattgaatc
     2881 gcatgtagcc ataactctct acgatgaaaa aagcgtattc agctaataat ctgatcgaag
     2941 cccagatgat gcttcaatta ttagagcaag cctatattcc ggccaaactg atgaatactt
     3001 tctcacaagg cggtttagga gaaattccct ttacccatgc ttaccctgaa gtttgggtga
     3061 tacgcgatga tgattttgag aaggcgcaga acatcattga tcaatatgag cacacttcga
     3121 ttgaccccgg acacgtaaac tgccccaatt gtagtgaatc caatccagcc aattttgaat
     3181 tatgttggaa ttgtggtcat gggctagaaa ataagggtta aatgaagacc acgttctacc
     3241 tccaagtttt attgcccaaa ctgtcctgac tgctgattgg aacttcattt caaaattaac
     3301 aatcgttagc agttcatctt gaattcttga aaaagaagga gcaaaaccaa cgaaattgga
     3361 atttttatca tggcaactta agataaaatt taacatccca aatcaataaa tatccgatta
     3421 acgcatgttt ttgcaaaacc tggcttcagc aggcagtttc ttagttatgc tgactattat
     3481 ttcttgcggt tttgcaaata tcgctacagc ttcaaatact tcaacaccag ctggcaaagc
     3541 ttcgatggat ccactaatgg atttaagtat cgaagaattg atacaaatta aaattacatc
     3601 agtgtcccgg cgcgaacaaa aattatctgg cacgccctcc gcaatttttg ttatcacaca
     3661 agaggatatt cgccgctctg gcgcgactag tattccagaa gcattgcgca tggctccggg
     3721 tgtcgaagtt gcccgggtgg gcacggataa gtggtcaatc agtgttcggg gcttcaatgg
     3781 tcgatttgca aataaactac aagttttaat ggacgggcgt agtgtt
//
LOCUS       DAOQLJ010000195         3824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_12194_length_3824_cov_4.930751, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000195 DAOQLJ010000000
VERSION     DAOQLJ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12194_length_3824_cov_4.930751"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="PK522_10665"
     CDS             <1..1483
                     /locus_tag="PK522_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNC42236.1"
                     /translation="QLAYVNSKLQTGITNLLDEAIIVACEQSNITLTGYQKLDEIPYD
                     FVRKRLTTVAKHEMDATATMICKGAFQSIIEICDQIQLASHIEPLTSEHQQQLLQHYT
                     QWSMQGYRVLAVAQKSVAINNNYTRDDEHSFVWIGFLLFFDPPEAGITDTLKALTQLG
                     VQLKIITGDNRYVACHVAEAVGIDTQTIVTGQALADMKDEALWHLAPQAGVFAEVDPN
                     QKERIITALQKTGHVVGYLGDGINDAPALHAADVGISVENAADVAREAADFVLLKHDL
                     NVLCQGIDEGRHTFANTLKYIFITTSANFGNMISMALASLFLPFLPLLAKQILLNNFL
                     SDIPAMGIAGDRVDRNWERTPHRWDVTLIRNFMITFGLVSVVFDLLTFGVLLYLAGEV
                     KAIFRTGWFVESLLTELLIIFVIRTYKPFYRGQPGRFLIWSVIGIALIALLLPYVPIG
                     SLFSFVPLPLPVMIAIMIITLLYVITSEVVKQLFFKRLNRKSL"
     gene            1838..>3824
                     /locus_tag="PK522_10670"
     CDS             1838..>3824
                     /locus_tag="PK522_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNC42237.1"
                     /translation="MITAFQAKYFAHELTRRFPTDSAEKLAGAVASAQVDINLHQVDA
                     ALFAFNSPLSKGALLADEVGLGKTIEAGLVLSQKWAERKRRILIITPSNLCKQWHQEL
                     NEKFFLPRYILEAKSYNAAIKQGQFRPFESPEIIICSYQFAKSKAADIHAIPWDLVVI
                     DEAHRLRNVYKPSNVIANTLKMALAGKHKLLLTATPLQNSLLELYGLVSFIDEHTFGD
                     LKSFREQFANLNQEQVFQTLKARLKAVCHRTLRRQVTAYIPYTKRLPLVEEFTPEDSE
                     DRLYHLVSEYLQRDNLQALPSSQRSLITLVLRKLLASSTFAIAGALTSISTRLKRKLN
                     KQEPEESLEDELDQDYEALDETAEEWTDDEPAELLTEDDRVALEQEITELDAFAALAI
                     SIDHNAKGKALLKALGIAFAKAKELGAAEKAIIFTESRRTQNYLLRLLADSPFAEGIV
                     LFNGTNTDDQSKVIYNQWLERHQGTDRVTGSRTADMRSALVDYFRDQGRIIIATEAGA
                     EGINLQFCSLVVNYDLPWNPQRIEQRIGRCHRYGQKHDVVVVNFLNRKNAADKRVFEL
                     LAEKFKLFEGVFGASDEVLGAIEAGVDFEKRIAAIYQHCRMQEEIQAAFDQLQLELSL
                     EINESMTRTRQQLLDNFDDEVREKLKVRDETSKAYLNH"
BASE COUNT         1086 a          824 c          843 g         1071 t
ORIGIN      
        1 gcaattagct tatgtgaatt ccaagttaca aaccggtatc accaatttac tggatgaagc
       61 tattatcgta gcgtgtgaac aatcgaacat cactctaacg ggttatcaaa aattagatga
      121 gattccctat gattttgtcc gtaagcgcct tactacggtg gctaaacatg aaatggacgc
      181 aactgcaacg atgatttgca agggcgcatt tcaatcgatt atagaaattt gcgatcaaat
      241 tcaattagct tctcacattg aaccattaac ttccgaacat cagcaacaac tgctccaaca
      301 ctatacacaa tggagtatgc aaggttatcg cgtgttagca gtggcccaga aatcggttgc
      361 aatcaataac aattacacac gagacgatga acatagcttt gtttggattg gttttttatt
      421 attttttgac ccaccggaag ctggaattac cgatacactc aaggcgttaa cgcaattagg
      481 tgtccagctt aaaatcatca ccggagataa tcgttatgtc gcttgccatg tcgctgaagc
      541 ggtaggaatt gatactcaaa cgattgtaac gggtcaagca ctcgccgata tgaaagatga
      601 agccctatgg catcttgcac cgcaagcggg ggttttcgca gaagttgatc caaaccagaa
      661 agaacgcatt attaccgcat tacaaaagac agggcatgta gtgggctatc tgggcgatgg
      721 tatcaacgat gcaccggctt tgcatgccgc tgatgtcggc atatcggttg aaaatgctgc
      781 cgatgttgcc agagaagcgg cggattttgt cttgctgaag catgatctca atgtgttatg
      841 ccaaggcatc gatgaaggtc gccatacctt tgctaatacg cttaagtata ttttcatcac
      901 gaccagtgcc aatttcggca atatgatcag tatggcattg gcttcgctat ttcttccttt
      961 tctcccttta ctcgctaagc agattttgct caataatttt ttatccgata ttccagcgat
     1021 gggcattgca ggtgaccgag tagatcgtaa ctgggaacgt actccccatc gttgggatgt
     1081 gacgctgatt cgaaatttta tgattacttt tggtttggtt agtgtcgtgt ttgatttact
     1141 cacatttggc gtattgcttt atttggcagg agaggtcaaa gcgatatttc gtacaggctg
     1201 gtttgtcgaa tcgctattga ccgaattgct cattattttc gtcatccgca cctataagcc
     1261 attttatcgt ggtcaacctg gccgttttct tatttggagt gttatcggta ttgcattgat
     1321 cgccttattg ctaccttatg taccgatagg aagcttattc agttttgtcc cactcccatt
     1381 accagtcatg atcgcgatta tgatcattac cctactctat gtaatcacct cagaggttgt
     1441 gaagcaatta tttttcaagc gtttaaatcg taaatccttg tagtaattac taatcattag
     1501 ctgataaagt cggtaatttt ttaaaggtat attaattcgc tgctgaccag ttcatggaaa
     1561 aaataccatg acagctaatc tcactgggta cccttggtta caagaatggc aattcaagga
     1621 ctgttagcag aaagagatga tggaatgcgg gtttgtggtg actattaaca ccccacctgg
     1681 atagatcagg ttcaaagaaa tagtctgata taaactccca caaattgaat tattctgaat
     1741 atgtacagta catttctata tacttaaaat agcggaaatt ctttttaaat cattctggtc
     1801 gatatgaatt ggaatacttg gtgatagact tcggctaatg attacagctt tccaagcaaa
     1861 atactttgcg cacgaactga cgcgtcgttt tccaacagat agcgctgaaa aattagcagg
     1921 tgcagtagcc agtgctcaag tggatatcaa tctgcatcaa gttgatgcag ctttgtttgc
     1981 ttttaactcg ccactttcta agggtgcgct actggctgat gaagtgggat taggcaagac
     2041 tattgaagct ggattggtgc tgtctcaaaa atgggccgag cgtaaacggc gtattttgat
     2101 cattacacct tcaaacctgt gtaaacaatg gcaccaggag ttgaacgaga aattctttct
     2161 cccgcgctat atcctcgaag caaaatccta taacgctgca atcaaacaag gccagtttcg
     2221 tccatttgaa tcacctgaaa tcataatttg ctcataccag tttgcaaaaa gtaaggctgc
     2281 agatattcat gcaatacctt gggatctggt tgttattgat gaagcgcata gactgcgcaa
     2341 tgtttataag ccatccaatg tcatagcaaa tacattgaag atggcattgg cgggaaaaca
     2401 taagctactg cttacagcga caccactaca aaattcactt ctggaactct atggattggt
     2461 gagttttatt gatgaacata ctttcggtga tttaaaaagt tttcgcgagc aatttgccaa
     2521 tttgaaccag gaacaagtat ttcaaaccct caaggctcgg ctcaaggcgg tttgtcatcg
     2581 aaccctgcgt cgtcaggtca ctgcctatat cccatacacc aagcgcctgc cgttggtaga
     2641 ggaatttact ccggaagata gcgaagatcg tctttaccac cttgtttcag aatatctgca
     2701 gcgtgacaat ctacaagcgc tgccatcaag ccagcgttcc ctgataacac tggtgttacg
     2761 caaattgctg gcctcatcca cctttgccat tgcgggtgcg ctaacttcga tttcaactcg
     2821 actcaaacgc aagctgaaca aacaagaacc tgaagaatcg ctcgaagatg aactggatca
     2881 ggactacgaa gcactcgatg aaactgccga ggaatggacg gatgacgaac ctgccgaatt
     2941 gctcaccgag gatgatcgtg tggctcttga gcaggaaatt accgagcttg atgctttcgc
     3001 cgcgcttgcc atctcaatag accataatgc caagggtaag gcgctgctaa aagcactggg
     3061 tattgcattt gccaaggcaa aagaacttgg cgcagccgag aaagcaatca tctttaccga
     3121 atcacgccgc acacaaaact atttattgcg cttactggcg gacagtccat ttgctgaagg
     3181 aattgtactt ttcaatggca ctaataccga tgatcaatcc aaagttattt acaatcagtg
     3241 gcttgagcgt caccaaggca ccgaccgagt aacagggtcg cgcaccgcag atatgcgctc
     3301 agcactggtc gattatttcc gtgatcaggg acgcatcata atcgcaacgg aagccggtgc
     3361 cgaaggtatc aatcttcaat tctgctcact ggtcgttaat tatgatctgc catggaatcc
     3421 gcagcgcatc gagcaacgaa ttggtcgttg tcaccgttat gggcaaaagc acgatgtggt
     3481 ggttgtaaac tttcttaacc gcaagaatgc tgcagacaag cgcgttttcg aattattggc
     3541 agaaaaattt aaactctttg aaggtgtttt tggtgccagc gatgaagtgc tgggcgcaat
     3601 tgaagcgggt gttgatttcg aaaagcgcat agccgccatc taccaacact gccgcatgca
     3661 ggaagaaatt caagcagctt ttgatcagct ccagctcgaa ttgagtttgg aaatcaatga
     3721 atctatgaca cgtacgcgcc aacaattatt agataatttt gatgacgagg tgcgtgaaaa
     3781 gctcaaagta cgggacgaga catctaaagc atacctcaat catt
//
LOCUS       DAOQLJ010000196         3742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_12650_length_3742_cov_5.704367, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000196 DAOQLJ010000000
VERSION     DAOQLJ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12650_length_3742_cov_5.704367"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PK522_10675"
     rRNA            <1..319
                     /locus_tag="PK522_10675"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            379..491
                     /gene="rrf"
                     /locus_tag="PK522_10680"
     rRNA            379..491
                     /gene="rrf"
                     /locus_tag="PK522_10680"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1301..2248)
                     /locus_tag="PK522_10685"
     CDS             complement(1301..2248)
                     /locus_tag="PK522_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42238.1"
                     /translation="MNTNLIDSSQEVLSDDSSNKQYTFTIENGVVKAYFEVENGVSEQ
                     ESIDNRDTFVINGNQITHTKQTSNGLETEVFVDSDGDGFYTRTSQSNQNGNDDDSNDD
                     NGNDDNGNDDDSSGNHKVFKFEINNGQVTAVFELKNGVLEPKSLDDNGTKSYTVSGND
                     VIRTEIKPFGTEITRYSDADGDGLFVRVSEQWQVSTNPSGVVPRFTETITFTPTSNDD
                     NIAVRGGEHCRGGQGADDFIFREADHLRIEDFSSQTGDALIFDTGLGLTSKEHLASFI
                     TDIHHDGQNFIVNFGSTVSITLIGVQPDQISWDDVSVLS"
     gene            complement(2542..3522)
                     /locus_tag="PK522_10690"
     CDS             complement(2542..3522)
                     /locus_tag="PK522_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNC42239.1"
                     /translation="MHFNQQPLLLFIGLFFFAITSLLVGISFGSVDISWSEIAQILSN
                     PSEEPHAIIIWELRLPRVLTAFACGSLLALAGALLQVLLRNPLADPYILGISGGASVG
                     ALTAMMLGWSTTSINIASSAGALTIIGLIFSLNFRSPTWNIYRLLLIGVVLSAACNAV
                     ITLLLALAPAHDTKGMLFWLMGDLSRSQASLSTWGVLIILCIISTLLASEFNVLSIGQ
                     LKARSLGIAVNILQILIFLIASIATVTALMQAGAIGFIGLLMPHAIRLVGVNDYRWLI
                     PCVILLGGGFLVFADTLARTLWAPQQLPVGVLTALLGAPLLLILLIRKRF"
     gene            complement(3579..>3742)
                     /gene="rnpB"
                     /locus_tag="PK522_10695"
     ncRNA           complement(3579..>3742)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK522_10695"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1137 a          743 c          721 g         1141 t
ORIGIN      
        1 ttaaaacgtc gtgagacagt ttggtcccta tctgccgtgg gcgttggaaa tttgaatggg
       61 gctgttccta gtacgagagg accggaatgg acatacctct ggtgtaccgg ttatgacgcc
      121 agtcgtacag ccgggtagct aagtatggaa aagataaccg ctgaaagcat ctaagcggga
      181 aacttgccat gagataagat ttccctggga aaatattccc ctaaaggctc gttgtagatc
      241 acaacgttga taggtcgagt gtgtaagcgc agtaatgtgt ttagctaatc gatactaatt
      301 ggccgtgagg cttgatccta taaatatatt attactaaat tacttataaa tattgaatat
      361 ttatatatta aagttttgct tggtgaatat agcgctttgg aaccacttct tcccataccg
      421 aacagaatcg tgaaacgaag ttgcgccgat gatagtgtgt atacgcatgt gaaagtagga
      481 catcgccaag cttttcatat ttagcaaaat tgattgaatg attgtgaagt gagattagat
      541 ttaattgaga ttttttctgc ttcaaatcat tttcccactg agctaaatat tcataggatt
      601 atttcatgat aaatccttcc atcattataa taatcgattc agctgcctaa gtctttttgc
      661 aatgactcca aaaaatggca gcatcttatt atttaagata tcagctgcag taataagcgt
      721 tttcgccgta tagaaattgc aatacgctct tcagatttat cacataaaaa agtaccctgt
      781 gcaccaaaat aaacaggata cgctgtttct atttcaacgt ggcgatgata tcattctgtt
      841 tctttttttc aatgctacga tttgtgcgtc attcaaaatg acgccttcac tggcgatctt
      901 ggtttctagt gcagcaagga gttctttgtt gtttgctact acctgataca taaagatgtt
      961 ggctatgtgt tggatattct accgcatagg ttaccaccgc cctattagca atacgacgcg
     1021 gatcctgttg atcaatgctt ccagaccatt atctttcagg tttttgagac gttctggagt
     1081 tcttcggcaa gattgagcaa tcctgtttta tgattggatt gttagtttga ttttaaattt
     1141 caacatctat atcaaacctt agctttattt tcctgcaagc aaattttcat tttctgatag
     1201 ataattaatg ctaacgcatt gaattattta ttttaaaata acttaactta cttctagact
     1261 aacagatcta gtctgctagc ctagagcccc atacttttaa ttagctaaga acggaaacat
     1321 cgtcccagct tatttgatct ggttgcactc caatcaatgt aatggataca gttgacccaa
     1381 aatttactat aaaattttgt ccatcatgat gaatatcggt gataaagcta gctaagtgtt
     1441 cttttgatgt taagccgagt ccagtatcaa agattaaagc atcaccagtt tgtgaactaa
     1501 aatcttcgat acgaaggtga tcggcttcgc gaaaaatgaa atcatctgca ccttgcccac
     1561 cgcgacaatg ttctccacca cgaacagcga tattgtcatc attgctggtt ggggtaaatg
     1621 taatcgtttc agtaaatctg ggaacaacgc cagatgggtt tgtagagact tgccactgct
     1681 cggatacgcg aacaaaaagg ccgtcaccgt ccgcatcgga ataacgagta atttcagtgc
     1741 caaatggttt aatttcagta cgaattacat cgttgccact caccgtatag cttttcgttc
     1801 cattatcatc taaggattta ggctcaagta cgccattctt caattcaaat actgctgtta
     1861 cttgaccatt gttgatctca aacttgaaaa ctttgtgatt tccagaggaa tcgtcatcat
     1921 ttccgttatc gtcgttcccg ttatcatcat tactgtcatc atcattgcca ttttgattcg
     1981 attgagaagt acgggtatag aaaccatcgc catcggaatc gacaaagact tctgtttcaa
     2041 gtccattgga agtttgtttg gtatgtgtga tttgattgcc attaatcacg aatgtatctc
     2101 tgttatcaat tgattcttgt tcggaaacac cattctcaac ttcgaagtag gcttttacta
     2161 cgccattctc aatcgtaaat gtgtattgtt tattactact atcgtccgat aaaacttctt
     2221 ggctcgaatc gattaaatta gtattcattt tgtttctcct agttattagt aagaaagtgg
     2281 atagagaaag caaaagaatt tttattatta cttttttcta tcctttacat aaatatagaa
     2341 agcgtttgca tctcgttgcg gatttgctaa acccaatgaa ttacggatta gtgggttgag
     2401 aaatcatgtc taccgagata gtcagactct ccatcattct tatggtcgaa tccagtgcat
     2461 ttcatcaatt tctattttat gtgtgaaaaa ttcccatgtc aaatatattt ttagcccttt
     2521 ttttatctac ccaatcataa tttagaagcg ttttcttatt aatagaatga gcagcagcgg
     2581 agcaccgagt agggcggtta atactccaac aggtagttgt tgaggggccc ataatgtacg
     2641 tgccagtgtg tctgcaaata ctagaaatcc accgcccagt aatattacac agggaatgag
     2701 ccagcgataa tcatttacgc caactaaccg aattgcatga ggcattagta acccaataaa
     2761 accaatagca ccggcttgca tcaatgctgt tacggtagca atagatgcga tcagaaaaat
     2821 taatatttgt aaaatattga cggcaatgcc gagagaacgt gctttaagtt ggccgatact
     2881 tagtacattg aattcacttg caagtagtgt actgataatg cacagaataa ttaagacacc
     2941 ccaagtagat agtgatgctt gagaacggga aagatcaccc attaaccaaa ataacatccc
     3001 tttcgtatca tgtgcaggag caagtgctaa taaaagagta atgactgcat tacatgccgc
     3061 agatagcact accccaatta acaataagcg ataaatattc caagtcggtg aacgaaaatt
     3121 caaactaaaa attaatccta taatcgttaa cgcacctgct gatgacgcaa tattgatact
     3181 agtcgttgac catcccagca tcattgcagt taaagcaccg actgaagcac ctccagaaat
     3241 acccagaata taaggatcag caaggggatt gcgcaataag acttgtaata aagctcctgc
     3301 aagagcaagt aagcttccac acgcgaaagc ggttagtact cggggtaatc gcaattccca
     3361 aataataata gcatgcggtt cttcactagg attgctcaga atttgagcaa tctctgacca
     3421 gcttatatca acactgccaa atgatatgcc aaccaaaagg ctagtgatcg caaaaaagaa
     3481 taaaccaatg aaaagcagca aaggttgttg attgaaatgc actgattcgt gatctaccgt
     3541 atagtatagc gtagatttat aaagaaatta ttcgtagggg gagaagctga ccgataagcc
     3601 gggttctgtc atggacagtc attcctctag gcatacggtt acccatacac tcaagcaacc
     3661 tacccgcaga ttcggcgagc aacgtcaaca cctgcctatt tggtcttgct ccggatggag
     3721 gttaccgcgt ttcaccgtaa ct
//
LOCUS       DAOQLJ010000197         3702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_12870_length_3702_cov_6.914999, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000197 DAOQLJ010000000
VERSION     DAOQLJ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12870_length_3702_cov_6.914999"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="PK522_10700"
     CDS             complement(<1..1287)
                     /locus_tag="PK522_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42240.1"
                     /translation="MQLIRDDRKISIIGGLLLLCLTLATGMAVYNVMRQQIESTLGRG
                     LDVALQGKARLFESQISEGLAATRATVNRPFLIRSLEQIKEQPNNIRALQDLQRNVDS
                     LILADFTAASISNSDGKELARVGKFSSDHTPKLPISIRSSAWLYWNDGFVLSVTKDIL
                     DQAGTRVGSLTTEKSLPQLTESFSKIWEIGKTGEFIVCAPPNEASDQMHCLISQISGV
                     EFRFLSRVIRNEALPMDYALRDQRGVIASKDYRHIPVIAAYAPLESVELGMVLKLDEE
                     ELLKPMNEQLKVIILYISGLIITEILLLYWLVHKLIRAIRGSRKAKERAEQFSTELAH
                     KELALRERFKEITCLYEIGSNMKSEFSIQEICQLVFKHLIGAMQYPDDASAFIELDGI
                     QYMSDNFQCEGTHFLQSKIELDGKICGHLYVFYPYDK"
     gene            complement(1440..2051)
                     /locus_tag="PK522_10705"
     CDS             complement(1440..2051)
                     /locus_tag="PK522_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNC42241.1"
                     /translation="MQTLWYIVDPMCSWCWGFSPIIETIREIYKNQLQIELIVGGLRA
                     GTTEPLHPSKRSEILQHWQNVQHRTNQPFKFENALPEGFVYDTEPACRALVTAATIDS
                     ACIFALLHTIQNAFYAEQLDVTRTEILSQLAVNVGIQPERFIATYNSDTLKLKTQQQF
                     QQAMQWGVSGFPSLVYLKNNVPNFLMRGYCDLHELQTRITTVL"
     gene            complement(2101..>3702)
                     /gene="typA"
                     /locus_tag="PK522_10710"
     CDS             complement(2101..>3702)
                     /gene="typA"
                     /locus_tag="PK522_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNC42242.1"
                     /translation="IVDTPGHADFGGEVERVLSMVDGVLLLVDAVEGPMPQTRFVTKK
                     ALALGLRPIVVINKVDRPGARPDWVINHTFDLFDKLNATEAQLDFPVVYASALNGYAT
                     TDLTQPSTDMRPLFDTILKHVPSPGGNPDEPLQLQISALDYSSFVGRIGVGRITRGRL
                     KPAQEVMIMTGNQTPKKAKVNQVFGFQGLERVPLSEALAGDIVQINGIEELSIGTTLA
                     DIDHPEALPMPKVDEPTLSMTFQVNTSPFAGKEGKFVTSRQIRERLEKELLTNVALKL
                     EETTDTDSFLVSGRGELHLTILIENMRREGYELAVSRPRVVIREIDGVKCEPFEVLTV
                     DVDEENQGAVMEALGARRGDLLDMASDSRGRVRLDYRIPARGLIGFQSDFMTMTRGTG
                     MMSHVFDDYGPMRPEIAARRNGVLISAEHGEAVAYALWKLQDRGRMFVNPGDPVYEGM
                     VIGIHSRENDLVVNPIKGKQLTNIRASGHDEAVALIPPIQLTLESAVEFIADDELAEI
                     TPKSIRIRKRHLQEHERKRAARSAA"
BASE COUNT         1009 a          830 c          807 g         1056 t
ORIGIN      
        1 cttatcgtaa ggataaaaaa cgtataagtg accgcagatt tttccatcta actcaatttt
       61 tgattgcaga aaatgggtac cttcgcattg aaagttatct gacatatatt gaataccatc
      121 taactcaatg aatgcagagg catcatctgg atattgcatc gccccgatca aatgcttgaa
      181 aactaattga cagatttctt ggatcgaaaa ttctgatttc atgttactgc cgatttcata
      241 aagacaagta atttccttaa accgttcacg taatgcaagt tctttgtgag ccagttcagt
      301 agaaaactgc tccgctcttt ctttagcttt tcgcgatcca cgtatcgctc tgataagttt
      361 atgaacaagc caataaagca ataaaatctc agtaatgatc aatccagaaa tatacaagat
      421 gatgacttta agctgctcgt tcattggttt aagcagttct tcttcatcaa gctttagcac
      481 cattccaagt tcaaccgatt ctaatggtgc gtatgctgca atgacaggaa tatgacggta
      541 gtcttttgaa gcgataaccc ctctttggtc gcgtagcgca taatccatcg ggagtgcttc
      601 attacggatg acacgggaca ggaatctaaa ttctacccct gatatttgac taatgaggca
      661 atgcatttga tctgatgctt cattaggtgg cgcacaaaca ataaactccc ctgttttacc
      721 aatttcccat atcttgctaa aactttcagt taattgaggt aggctttttt cagttgtaag
      781 actgccaact cttgtcccag cttggtcgag aatatctttg gttacgctga gtacaaatcc
      841 atcattccag tatagccatg cgctagatcg tatgctaatg ggtaatttag gtgtgtgatc
      901 cgatgagaac ttgccgacac gggctaattc cttgccatcg ctatttgaaa tcgatgccgc
      961 tgtaaaatca gcgagtatta atgaatccac attgcgttga agatcttgta gtgctctaat
     1021 gttattgggt tgttccttaa tttgctcaag cgagcgaatc agaaaggggc gatttacagt
     1081 agcacgggtg gctgctaggc cctcagaaat ttgactttca aacaaacggg ccttaccttg
     1141 caaagcaaca tcaagccctc gtcccaaggt agattcaatt tgttgacgca tcacgttgta
     1201 aacagccatt ccagtggcta atgtcaagca gagtaggagt aggcctccta tgatgctgat
     1261 cttgcgatca tctcgtatta gctgcaaatc attctcctat caaatagtct gatcctaaag
     1321 tcatcgctga tcgcttgttg cgtatgagat tataggagta aatgatctgg ggttgttaca
     1381 acctatttta ggagttgggt aaaaacctag aattcaattc aaatttttaa tgcttcacat
     1441 tacagcactg tcgtgatgcg cgtttgcaat tcatggagat cacagtagcc acgcataaga
     1501 aaattaggga cattattttt caaatatact aaagaaggaa atccgcttac accccattgc
     1561 atggcttgct ggaattgttg ctgtgtttta agtttgagtg tatctgagtt gtaggtggcg
     1621 ataaatcgct cgggttgaat gcctacatta acagcgagct gactcagtat ttctgtgcga
     1681 gtaacatcaa gctgctcagc gtagaaggca ttttgaatcg tatgtagtaa cgcaaaaata
     1741 caggctgaat caatggttgc cgcagtgact aacgctcggc aagcaggttc tgtatcgtaa
     1801 acgaaacctt caggcagagc attttcaaat ttaaagggct gattggtcct atgttgaaca
     1861 ttttgccagt gctgaagtat ttcagaacgt ttgctaggat gaagtggttc tgtagtaccc
     1921 gcacgtaatc caccgacgat taattctatt tgaagctgat ttttatagat ttccctgatg
     1981 gtttcgataa taggtgaaaa cccccagcac caggaacaca tagggtcaac gatgtaccac
     2041 agcgtttgca tcaagaagta cttaggatgt aaatccgctg tggcttatat ctgctagcga
     2101 ctaagccgct gagcgtgcag cacgtttacg ctcatgttct tgcaagtggc gtttacgaat
     2161 tcgaatactc ttgggtgtaa tttctgctaa ttcatcatca gcaatgaatt caacagccga
     2221 ttcaagcgtg agttgaatgg ggggtattaa agccacggct tcatcatgtc cagaagcgcg
     2281 gatattggtt aattgctttc ctttgatcgg gtttaccacg agatcatttt cacgactatg
     2341 aattccaata accatgcctt cataaacggg atcgccagga ttaacgaaca tacgtccccg
     2401 atcctgtagc ttccatagag cataagctac cgcttcgcca tgctcggcag aaattaggac
     2461 cccatttctt cgggccgcaa tttctgggcg catgggtcca taatcgtcaa atacatgact
     2521 catcatgccg gttcctcgcg tcatcgtcat gaaatctgat tgaaaaccga tcaggccgcg
     2581 ggcgggaata cgataatcga gcctgactcg cccacgacta tcagatgcca tatcaagaag
     2641 atcaccacga cgagcgccta atgcttccat tacagcgccc tgattttctt cgtcgacatc
     2701 aacggtgagt acctcgaagg gttcacattt aaccccatct atttctcgaa taacgacgcg
     2761 aggacgtgat accgctaatt cataaccctc acggcgcata ttttcgataa gaatggttaa
     2821 gtgtaattca ccacgtcctg aaactaagaa tgaatcggta tcggtagttt cttctaattt
     2881 cagtgctaca ttcgtcagta attctttttc aagtcgttca cgaatttgtc ggctcgtgac
     2941 aaatttccct tcttttccgg caaacggcga ggtgttgacc tggaatgtca tcgacagcgt
     3001 tggttcatct actttgggca tgggtaacgc ttcagggtga tcaatatccg cgagcgtggt
     3061 accgatgcta agttcttcaa tgccattgat ttggacgatg tcaccagcta acgcttcgct
     3121 aagcggtaca cgctccaaac cttgaaaccc aaagacttgg ttcacttttg cttttttcgg
     3181 tgtttggtta ccagtcatga tcatcacttc ttgcgcaggt ttaaggcgac cacgcgtaat
     3241 ccgaccaaca ccaattcgac cgacgaagct cgagtagtcc aatgcactga tttgaagttg
     3301 caagggttca tcaggattgc cacctggact cgggacatgc ttgaggatag tatcaaataa
     3361 aggccgcatg tcggtacttg gttgagtaag atctgtggtg gcataaccgt tcagcgctga
     3421 agcatagacc acaggaaaat cgagctgagc ctcggtagca ttgagtttgt caaacaagtc
     3481 gaaggtatga ttgatgaccc aatctgggcg cgcgccaggt cggtcaactt tgttaatcac
     3541 gacgataggt ctcaatccta gcgcaagcgc cttttttgtt acaaaacgcg tttgtggcat
     3601 cgggccttct accgcatcga cgagcagcaa aacaccatcc accatcgaga gaacgcgctc
     3661 cacttcacca ccaaaatcag catgaccggg cgtatcgacg at
//
LOCUS       DAOQLJ010000198         3700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_12888_length_3700_cov_6.569547, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000198 DAOQLJ010000000
VERSION     DAOQLJ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12888_length_3700_cov_6.569547"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1138)
                     /gene="pheS"
                     /locus_tag="PK522_10715"
     CDS             complement(119..1138)
                     /gene="pheS"
                     /locus_tag="PK522_10715"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNC42243.1"
                     /translation="MVEIDGLVNEAIALLNKAGTIIELDQVKVRYLGKEGELTKLLKG
                     LSKLSQDERPAAGNRINQGKKQVEEALNQRRAILLENEVQIRLAADNIDVTLPGRGLS
                     RGGLHPVSIVLERIERLFRSIGFSVASGPEIETDFYNFTALNIPQNHPARAMHDTFYV
                     ENGDLLRTHTSPVQIRFMAEYKPPLKVIAPGRVYRCDSDVTHTPMFHQVEGFWVDEEV
                     NFSALKGILMTFMQNFFEREDLKVRFRPSFFPFTEPSAEMDISCVMCNGSGCRVCSHT
                     GWLEVLGCGMVHPNVLKYVNIDSEKYTAFAFGVGVERLTMLRYGVSDLRLFFENDLRF
                     LAQFN"
     gene            complement(1241..1600)
                     /gene="rplT"
                     /locus_tag="PK522_10720"
     CDS             complement(1241..1600)
                     /gene="rplT"
                     /locus_tag="PK522_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNC42244.1"
                     /translation="MPRVKRGVTAHARHKKILKLAKGYRGRRKNVFRIAKEAVMKAGQ
                     YAYRDRRQKKRQFRVLWIARINAAVREHGLSYSNFMNGIKKAGIELDRKVLSHLAVFD
                     KAAFEEIVKKARTSLAV"
     gene            complement(1613..1810)
                     /gene="rpmI"
                     /locus_tag="PK522_10725"
     CDS             complement(1613..1810)
                     /gene="rpmI"
                     /locus_tag="PK522_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNC42245.1"
                     /translation="MPKMKTKKSAAKRFLVRASGSIKRSQAFKRHILTKKTTKNKRQL
                     RGTTHIHSSNIASVNAMLPYL"
     gene            complement(1951..2538)
                     /gene="infC"
                     /locus_tag="PK522_10730"
     CDS             complement(1951..2538)
                     /gene="infC"
                     /locus_tag="PK522_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNC42246.1"
                     /translation="MIQERSVRINEEINAPVVRLIGLKGEQLGVVSLTDANARAEEES
                     VDLVEIAPGADPPVCRLMDYGKYLYQESKKKHDAKLKQKQVQVKEIKFRPNTDEGDYN
                     IKLRSIINFLDDGDKVKITLRFRGREMAHQEFGVRLLDRIKEDLQTYGLVEQHPKLEG
                     RQMVMVLSPKKKEVKSAKSKESTKEKDKPETIISS"
     gene            complement(2560..>3700)
                     /gene="thrS"
                     /locus_tag="PK522_10735"
     CDS             complement(2560..>3700)
                     /gene="thrS"
                     /locus_tag="PK522_10735"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNC42247.1"
                     /translation="DEAPGMVFWHSKGWALWQQIEQYMRQVLNRSGYQEIRTPQVLDR
                     SLWEHSGHWENFRENMFVTESEERHFAIKPMNCPGHIQIFNQGLKSYRELPLRLAEFG
                     SCHRNEPSGALHGIMRVRAFTQDDAHIFCTEEQVQSEVIQFIDLLKQVYKDFGFDAIL
                     VKLSTRPQKRVGSEQQWDDAESALESALEAKKLDWQIQPGEGAFYGPKIEFSLRDSLG
                     RVWQCGTLQLDFSMPARLGATYVSEDNLKKTPVMLHRAILGSLERFIGILIENYAGAL
                     PVWLAPVQVAVLNISKNQMEYAQEIEKALSSNGIRVLLDLRNEKISYKIREHSLQKIP
                     YQIIVGDEEVNGNIIAVRNRLNKDMGQMTVANFIECIHADCMKKL"
BASE COUNT         1059 a          870 c          578 g         1193 t
ORIGIN      
        1 ccacttcaac acctgccatg gtaagtgtat gaactaattg atctctagaa taagggggat
       61 taacaaggct tcgcagccaa ttttctgaaa atctcatgat gagagaaacc tacaaaattt
      121 aattaaattg cgctaagaaa cgtaaatcat tttcaaagaa caaccgtaaa tcactgactc
      181 catagcgcag catcgttaat ctttccacgc ctaccccaaa cgcaaaagct gtatatttct
      241 cgctatcaat attgacatac ttgagtacat ttggatgaac cattccacag cctaatactt
      301 ccaaccagcc ggtatggcta caaactctgc aaccagaacc attgcacatg acacagctaa
      361 tatccatctc tgcagagggt tcagtaaatg ggaaaaaaga aggtctaaaa cgtactttca
      421 aatcttcacg ctcaaagaaa ttctgcataa aagtcattaa gattcccttt aaagcagaga
      481 aattcacttc ttcatccacc caaaatcctt caacctggtg aaacatcgga gtatgagtaa
      541 catctgaatc gcatcgataa acacgaccag gtgcaattac cttcaatgga ggtttatact
      601 cggccataaa acggatttga accggtgatg tatgcgtccg caacagatca ccattttcta
      661 cataaaatgt gtcgtgcatc gctcttgctg gatgattctg tggaatattt agtgcggtaa
      721 aattataaaa atcggtctca atctctggcc cagatgcgac agagaaacca atcgatcgaa
      781 acagccgctc aatacgctct aggacaatcg agacaggatg caatccaccc ctcgacaaac
      841 ctcgaccagg caaagtaaca tcaatattgt ctgctgctaa tcgtatctgt acctcatttt
      901 ctaatagtat tgcccttcgc tgattgagcg cttcttctac ttgttttttc ccctgattaa
      961 ttcgattacc cgctgcggga cgttcatcct gcgaaagttt gctcaaccct ttaagcaatt
     1021 tcgtcaactc gccttctttt ccaagatagc gaaccttgac ctgatcgagc tcaattatcg
     1081 tgcccgcctt gtttaatagc gcaattgcct cgttcacaag accatcaatt tcaaccataa
     1141 atcgtattat ttataataaa aaaacgatga aaacaaaaag ggagatcaag tcaaaatctc
     1201 cttgatctcc cttacaaaca ataccaatcc aataaactaa ttacaccgct aggctagttc
     1261 tagctttctt gacgatttcc tcaaaagctg ctttatcaaa cacggcaaga tgggatagga
     1321 ccttcctatc aagttctata ccagcctttt taattccatt cataaaatta ctataggata
     1381 gtccatgctc tctaacagct gcattgatcc ttgctatcca taaaactcgg aactgtcttt
     1441 tcttttgtcg cctatcccga taagcatact gacctgcttt catcaccgct tctttggcga
     1501 ttctaaatac attcttacgg cgaccgcgat aaccttttgc taacttaaga atttttttat
     1561 gacgtgcatg cgcagtcact ccacgcttta ctcttggcat tgctcccccg acttacaaat
     1621 aaggtaacat agcattaaca gaagctatgt tacttgaatg aatatgtgtg gttcctctta
     1681 actgtctttt atttttagta gtctttttcg ttaagatgtg tcgcttaaat gcttgtgatc
     1741 gcttaatact tccgcttgcc ctaacaagaa agcgcttagc agcacttttt ttagttttca
     1801 tttttggcac aataaatttt ctcctaattt ctaaaatctt tatttactga aatctataac
     1861 acaatacgtg aattctgaat cttaatgcta gccctcaact atgctcaatt ctggcctacc
     1921 gctattgggc ggtagagagg agttttacac ttaagatgaa ataattgtct ctggcttatc
     1981 tttctctttt gtcgattctt tagactttgc gctcttcacc tcttttttct taggtgataa
     2041 taccattacc atttgtcgac cttctaactt tggatgttgt tccactaaac cataagtttg
     2101 gagatcttct ttaattctat ccaagagccg cacaccaaat tcctggtgag ccatttctct
     2161 tcctctaaaa cgtagcgtaa tcttaacttt atctccatcg tccaagaagt taataatact
     2221 gcgcagtttt atattgtaat caccctcatc tgtattaggt ctaaatttta tctctttaac
     2281 ttgaacctgt ttttgtttta gttttgcgtc atgcttcttt ttactttctt gatagagata
     2341 ttttccataa tccattaacc gacaaactgg aggatcagcg cctggtgcaa tctcaaccaa
     2401 atcgacactt tcttcttctg cccgtgcatt tgcatcagta agtgacacta ctcctaactg
     2461 ctcccctttt aacccgatta atcttaccac tggagcattt atttcctcat tgatccttac
     2521 gcttctttct tgaattatag ttacttctcc ttataaaact tataattttt tcatacaatc
     2581 agcatgaata cattctataa aattagccac ggtcatctgt cccatgtctt tattcaaacg
     2641 atttcttaca gcaataatat taccatttac ttcctcatca ccgacgataa tctggtaagg
     2701 tattttttgc aaactgtgct cgcggatttt atagcttatc ttctcatttc tcaagtctaa
     2761 taatacccta attccatttg aactgagtgc tttctcaatt tcttgagcat actccatctg
     2821 attttttgaa atatttagaa ccgcaacttg aactggagca agccacacag gtagtgcgcc
     2881 tgcgtaattt tcgataagaa taccaataaa tcgttctaac gaacctaaaa ttgcgcggtg
     2941 taacattact ggagttttct ttaaattatc ttcactaaca taagttgcgc ccagtcgagc
     3001 tggcattgaa aaatctaatt gcaaagttcc acactgccac actcgcccga ggctgtctct
     3061 taaagaaaat tcaattttgg gaccataaaa tgcaccttcc cctggttgta tttgccaatc
     3121 cagtttttta gcttctaagg ccgattctaa ggctgattcg gcatcatccc attgctgttc
     3181 tgaacccact cttttctgtg gtcgagtaga aagttttact aagatcgcat caaatccaaa
     3241 atctttatag acttgtttca ataaatcgat aaattgtata acttcactct gaacttgctc
     3301 ttctgtacaa aaaatatgag catcatcctg tgtaaatgct cttacacgca taattccatg
     3361 caatgctcct gacggttcat tgcgatggca tgagccaaat tcagcaagtc gcaaaggtaa
     3421 ttctcgataa cttttaagac cctgattaaa tatttgtata tggcctggac aattcatcgg
     3481 tttaatggcg aaatggcgtt cctccgattc tgtcacaaac atattttccc gaaaattttc
     3541 ccagtgacca gaatgttccc acaaagatcg gtctaatact tgcggcgttc gaatttcctg
     3601 gtatccactt cgattcaata cttgacgcat atattgctcg atttgttgcc aaagcgccca
     3661 tcccttggaa tgccagaaaa ccataccagg tgcttcatct
//
LOCUS       DAOQLJ010000199         3685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_12969_length_3685_cov_6.463636, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000199 DAOQLJ010000000
VERSION     DAOQLJ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12969_length_3685_cov_6.463636"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1090
                     /locus_tag="PK522_10740"
     CDS             173..1090
                     /locus_tag="PK522_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNC42248.1"
                     /translation="MEIFFHQLILKLILINHQLPAMRLYSQPLSFINLILIILVISAT
                     PFSAQGNTIYKSTDAAGRVTYSDTPTPDARPIHLPSTPALTLHQVTTVLDGDTIVLKG
                     NQHIRLLGINTPETNTRYREAEAGGIVAKKWLQEKLTGVKVYLEHDQLKTDHYNRSLA
                     HVRTKNGEHINLILVEKGLAFVNLIPPNLRHADALISAQRKAEKEKLGIWAYPDYAPF
                     PITQIANKKTRSWQRFYGKPISIKRSKKYSRLFFSSNIDVRFENKYLTLFPSLETYLN
                     KTIEVRGWVSRRNDHYSISIQHPSAIVIR"
     gene            1227..1634
                     /locus_tag="PK522_10745"
     CDS             1227..1634
                     /locus_tag="PK522_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HNC42249.1"
                     /translation="MATIQSKIVADLETLYLENSSKNCPEEHVYEWLILGITIDGRTF
                     RPSDWAERLCGAIAQYNNGRLIYSELAYPISRNGQIGIVIETLLRSENPTIYSFFMDF
                     ARDNRLKIISGREAPRSLNTNSTQNNRPLRLVI"
     gene            complement(1694..2602)
                     /gene="ftsX"
                     /locus_tag="PK522_10750"
     CDS             complement(1694..2602)
                     /gene="ftsX"
                     /locus_tag="PK522_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNC42250.1"
                     /translation="MRIWISQHWQALGLTAKQFFFKPVSNFLSVAVIGIAFSFPLGIY
                     TLLDNLQSFSDHFVHSPQISLFLEIDATESDIENINSRLESFDGITSFQFVSNEIALA
                     QLQHESGMGDIALHLKKNPLPHAFIIHTQHMPPDKLEENRLTLQQWPAVSYVQVDSDW
                     TRKVHAFVKLGRVAVIMLSSLLGVALVIVIFNTIRLQILTKQEEIEVSKLIGATDRFI
                     RRPFLYFGALQGVVGALIAWIIITVGAQKINDNLTELSQLYELSLRLNPLSLFQVLLL
                     GAVAGFLGWLGARLSVAQHLSQIEPK"
     gene            complement(2599..3252)
                     /gene="ftsE"
                     /locus_tag="PK522_10755"
     CDS             complement(2599..3252)
                     /gene="ftsE"
                     /locus_tag="PK522_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNC42251.1"
                     /translation="MITFDKVYKRYPDGYEALTNLIFTIDTGEMVFITGHSGAGKSTL
                     LKLIAAIEKPTSGSITVSDQIVNKLKKSMVPFLRRSLGIIFQDQKILYDRTVFANVML
                     PLQIHGFEKSVMERRVRAALDKVGLLNKEKAYPITLSGGEKQRLCIARAVVHRPLILI
                     ADEPTANLDVAYAQNIMDVFRSFHQVGVTVLIATHDQTILNDSTARILSLNQGHLMV"
     gene            complement(3265..>3685)
                     /locus_tag="PK522_10760"
     CDS             complement(3265..>3685)
                     /locus_tag="PK522_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNC42252.1"
                     /translation="DPAAVIFDAINAAKARNIDIVLADTAGRLTTQMHLMEEIKKIKR
                     VINKALPDAPHEVLLILDANTGQNAISQVKIFNEALNVTGLVLTKLDGTAKGGVIAAI
                     AAQYPNNPPALRFIGIGESLDDLRPFDAIEFVEALFD"
BASE COUNT         1199 a          802 c          612 g         1072 t
ORIGIN      
        1 cacgcgacat agccatgtta acgttattca aaattggccg tgtgccatat gaaaaggcta
       61 gattcttgat ctcgatcaga ttatctgtca tgagattctt ctgttacttg taatatcacg
      121 aaaaaatagc attaagcata aaacaaggta ttctaattca gaacctgaat cgatggagat
      181 tttcttccat caattaatat tgaaattaat actgattaat caccagctac cagcaatgcg
      241 actttatagc caacctttaa gttttatcaa cttgatactc attattttag taattagtgc
      301 tactccattc agcgcacagg gcaatacaat ttataaatca accgatgccg caggacgagt
      361 cacctattcc gatacaccaa cacccgatgc aaggccgatc cacttacctt ccacaccagc
      421 actcacacta caccaggtca ctactgtact cgatggtgat acgatcgtgc tcaaaggaaa
      481 tcagcatatt cgcttattag gaattaatac tcccgaaact aatacaagat atcgtgaagc
      541 agaagctggt ggtatcgtcg caaaaaaatg gctacaagaa aaactaactg gagtaaaagt
      601 ctatttggaa catgatcaac tgaaaacaga tcattacaat cggtcacttg cgcatgttcg
      661 tacaaagaac ggggagcaca tcaatttaat tctagttgaa aaagggttag catttgttaa
      721 tttgataccc cccaatctcc gccatgctga tgccttgatt agcgcccaaa gaaaagcaga
      781 aaaagaaaag ctaggcattt gggcttatcc tgactacgca ccttttccga ttactcaaat
      841 tgcaaataaa aaaacacgta gttggcagcg cttttacgga aaacccattt caattaaacg
      901 tagcaaaaaa tacagccggc tcttttttag tagcaatatc gatgtccgtt ttgaaaataa
      961 atacctaact ttatttccat cattggagac ctatttgaat aaaaccatcg aagttcgtgg
     1021 atgggtatct cgtcgaaacg atcactactc aatatcaatt caacatccta gcgcaattgt
     1081 tattcgctag actaaattta gctgcttcct tgatttatag attttatctt tttatctgtc
     1141 agattatcta aatttcccaa gaatcttcta ttgtcagcca gattactgcc gttaagaatt
     1201 tgaaaaatta ggtagttgaa aaatatatgg caacaataca atccaaaata gtagctgact
     1261 tagaaaccct ctatctagaa aattcctcaa aaaattgtcc agaagagcac gtttatgaat
     1321 ggcttattct aggcatcacc attgatggcc gaacattccg accaagcgac tgggcagagc
     1381 gcctttgcgg cgcaattgca caatataaca atgggcgctt aatctattca gagttagctt
     1441 atccaatttc gcgcaacgga caaattggta tagtgattga aaccttacta agaagcgaaa
     1501 acccaaccat ctattctttt tttatggact ttgcccgaga taatcgacta aaaattatct
     1561 ccggtagaga agcccctcgc tcactcaata ccaactccac tcaaaacaat cgccctctcc
     1621 gtttagttat ttaattaagt gaaatctcat cacaaatctg accaatttta gtatgtctag
     1681 ctttataaat taactacttc ggctctatct gagaaagatg ctgagctaca gataaccttg
     1741 cacctagcca tccaagaaac cctgcaacag cgcctaacaa taaaacctga aaaagagata
     1801 aaggattcaa gcgtaaactt aactcataaa gttgtgacaa ttcagttaaa ttatcattga
     1861 tcttttgagc cccaacagta atgataatcc aagctatcaa agcacctacc actccttgca
     1921 atgcaccaaa ataaagaaag ggacgtcgta taaaacgatc cgttgcgccg atcagttttg
     1981 aaacttcgat ctcttcttgt tttgtaagta tttgcaaccg aattgtgtta aatatgacaa
     2041 ttactagcgc cacaccaagc aaagaagaca gcatgataac tgcaactctt cctaatttaa
     2101 caaaagcatg cacttttctg gtccaatcgg agtcaacttg aacatatgac accgctggcc
     2161 actgctgcaa agttagtcga ttctcttcta acttatcagg tggcatatgt tgcgtatgaa
     2221 taataaaagc atggggcaat ggattctttt ttaaatgaag cgcaatatct cccatcccac
     2281 tttcgtgttg tagctgcgct aaagcaattt cattggaaac aaattgaaag ctcgtaatac
     2341 catcaaaact ttccaatcgt gaattaatat tttcaatatc agactcagtc gcatcaatct
     2401 caagaaatag gcttatctga ggtgaatgta caaaatggtc tgaaaaactt tgtaagttat
     2461 ctaataaggt ataaatccct aacgggaagc taaatgcaat accaattact gccacgctta
     2521 gaaaatttga gacaggctta aaaaaaaatt gctttgcagt taaccccaaa gcttgccaat
     2581 gttgagaaat ccatattctc ataccataag atgtccttga ttgagtgaaa gtatacgcgc
     2641 agttgaatca ttcaaaatcg tttgatcatg ggttgctatt aataccgtaa ctccaacttg
     2701 atgaaatgat cgaaaaacat ccataatatt ttgggcatat gccacatcaa gatttgctgt
     2761 gggttcatca gcaatcaaga ttaaaggccg atgaacgaca gctcgcgcaa tacacaggcg
     2821 ctgcttttct cctccagata acgtaatggg atacgccttt tctttattta gtaacccaac
     2881 tttatctaat gctgcacgca ctcgcctctc catgacactc ttttcgaacc catgaatttg
     2941 tagtggtagc atcacattag caaatactgt gcgatcatat aaaatctttt gatcttgaaa
     3001 gataatccct aggctacgcc ttaaaaaagg aaccatgctt ttcttcagtt tattcacaat
     3061 ttgatcacta accgtaatgc ttccagatgt tggtttttca attgccgcaa tcaattttag
     3121 taacgtactt tttcctgcgc cagaatggcc agtaataaat accatttcac ctgtatcaat
     3181 cgtaaaaatg agattcgtta gcgcttcata gccatctgga taacgcttat aaaccttatc
     3241 aaaagtaatc attacaacgt aaaactaatc gaataaagcc tcaacaaatt caattgcatc
     3301 aaagggccta agatcatcta agctctcccc aataccaata aaacgtaaag ctggcggatt
     3361 gtttggatat tgcgctgcaa tcgctgcaat aacgcctcct tttgctgttc cgtcaagctt
     3421 tgttaaaacc agacccgtca catttaaagc ctcattaaag attttgactt ggcttatagc
     3481 attttgtccc gtgttagcat ccagtatgag caatacttca tgcggtgcgt caggcagcgc
     3541 tttattgatg acacgcttta ttttttttat ttcttccatt aagtgcattt gcgtagtcaa
     3601 tctacccgct gtatcagcca atacgatatc aatatttctt gcctttgccg cattgattgc
     3661 atcaaaaata acagctgcag gatca
//
LOCUS       DAOQLJ010000200         3684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_12972_length_3684_cov_5.266465, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000200 DAOQLJ010000000
VERSION     DAOQLJ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12972_length_3684_cov_5.266465"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PK522_10765"
     CDS             complement(<1..489)
                     /locus_tag="PK522_10765"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNC42253.1"
                     /translation="MKLAIADLETSGFYADSSILLCCSIKDYHTHKVTTLRADKYKNW
                     KTKKTLEKPFIEDVLNALDDYDILVFHNGEFFDKGYMNAKAVQYGLYPVLRAKKTIDP
                     VLVSRRHLRLGRNSLAALIDYLRVPVKKTPIELNVWTQAALEGDTDCMDKIVKHCEHD
                     VIT"
     gene            complement(551..754)
                     /locus_tag="PK522_10770"
     CDS             complement(551..754)
                     /locus_tag="PK522_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42254.1"
                     /translation="MSIKVISEKFHAPTPAKWRKVGFALLSVSSFISGCGVYSDVKWV
                     AYTGLATGVLGTFLTNLTSEESK"
     gene            complement(744..1001)
                     /locus_tag="PK522_10775"
     CDS             complement(744..1001)
                     /locus_tag="PK522_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42255.1"
                     /translation="METTVLLGIIQIILAALGGVLWSMFKDLKQKHEGMSKSFDAFKL
                     EVVKQYMPKSELQGVLDKFERKIEEGFKNLMDEIKEIKREH"
     gene            complement(1011..1502)
                     /locus_tag="PK522_10780"
     CDS             complement(1011..1502)
                     /locus_tag="PK522_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42256.1"
                     /translation="MKYIVITVIIVASMIIGALLQSKFSEDGSIALKTSDTVVVIKKD
                     TVRVVQTIFAKPITRIEYATKNDTIFISDTIFVFDSVRFAEMDTFIHRGLDSAYLNIR
                     HYIGNLDLFKVKTTWNPETYKEIIVTNTVIKKLDYGLVAGIGFDVKGNPNILVGIGVR
                     FKP"
     gene            complement(1782..1967)
                     /locus_tag="PK522_10785"
     CDS             complement(1782..1967)
                     /locus_tag="PK522_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42257.1"
                     /translation="MIKRKFKTDAPCEEVAALMEMTDVQLANYEEAHKEEWRSCLEAY
                     VPPEPGLWDQLVNWWNS"
     gene            complement(2018..2947)
                     /locus_tag="PK522_10790"
     CDS             complement(2018..2947)
                     /locus_tag="PK522_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42258.1"
                     /translation="MSKRIDELPDVGANSSLAHFFAAQASGGGAGTTYKYDGDALGSL
                     LGGNKTWLTSWIDQTPFGGFELSRMFYTTRWAEADASLNTQLGITTVDSGAAAQAPIT
                     NRGMLRTLTQTSAASAGGVRRSATNNNPMYQRGSAQGENGFLLIDIFLPFGTNWDNTG
                     VGTGTRWISGITSSATSTLAAALGSDNPTNSRLAFMRSHVNGGLTETNWWFSAKDGTT
                     ENRLDTGVAFSGNSWYATMIACAPQGSTVYFAIKNLTGGNDSGVLSTSSNLPAADTFL
                     YQWTGFYNIDAVTRICYPKSFISYVPNMLRAFL"
     gene            complement(3077..3397)
                     /locus_tag="PK522_10795"
     CDS             complement(3077..3397)
                     /locus_tag="PK522_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42259.1"
                     /translation="MTISGGIHGGIEVEFNPSGLLLDGREYFVFEGLFYAKNDDQLVF
                     VGDALPIDNNLATQEQQEPVVETTNINAPSEVIIEEVTSNLPPQESSQSSESFNLFKP
                     STWL"
BASE COUNT         1087 a          855 c          652 g         1090 t
ORIGIN      
        1 cgtgatgaca tcgtgctcgc aatgcttcac gatcttatcc atacaatcag tatcgccttc
       61 taatgcagcc tgagtccaaa catttagctc tattggagtt ttctttaccg gtacccgcaa
      121 gtaatcaatt aaagctgcta acgaattacg cccaagcctt agatgacgac gtgatacaag
      181 aaccggatct atcgttttct tagctctaag aacagggtat aggccatact gaacggcctt
      241 tgcgttcatg taacccttgt cgaaaaactc gccattgtgg aaaacaagaa tatcataatc
      301 atcgagagca tttaagacat cctctatgaa tggcttctca agtgtttttt ttgttttcca
      361 attcttgtat ttgtcggctc gtagtgtagt tactttgtgg gtgtggtaat ctttgataga
      421 acaacaaagt aatattgatg agtccgcgta gaatccggac gtctcaagat cagctatagc
      481 tagcttcatt tataactcca ttttatcatt cgctcaatat agcgaataac aaaaaggaag
      541 tcaagcataa ttatttagat tcttcactcg ttagattggt aaggaatgta cccagaacgc
      601 cggtagcaag gcctgtataa gcaacccact ttacatcgga ataaacgccg caaccactta
      661 tgaacgaact aacggaaagt agtgcaaaac ctaccttacg ccatttagcc ggtgtaggag
      721 cgtgaaattt ttcactgatt actttaatgc tcacgtttaa tctcctttat ttcgtccatt
      781 agattcttaa aaccctcttc tattttacgc tcgaatttat ccaatactcc ctgcaactcg
      841 gattttggca tatactgttt aaccacttcg agtttaaagg catcaaacga ttttgacata
      901 ccttcgtgtt tctgtttaag atccttgaac atagaccaaa gaacaccacc aagtgccgct
      961 aaaataattt gaataattcc aagaagaacc gtcgtttcca tgcaacccca ttaaggtttg
     1021 aatcttacgc caataccgac caatatatta gggttcccct ttacatcaaa accaataccg
     1081 gccactaatc cataatctaa ctttttgatt actgtatttg tgacgatgat ttctttatag
     1141 gtttccgggt tccaagtcgt tttaaccttg aataaatcca gattcccaat atagtgccgt
     1201 atattaagat aagcgctatc aagaccgcga tgaataaaag tgtccatttc agcaaatcgt
     1261 acactatcaa aaacaaagat cgtatcggag ataaaaattg tgtcattttt tgtcgcatat
     1321 tcaatcctag taattggctt tgcaaatata gtctggacaa ctctaactgt atctttctta
     1381 ataactacaa cggtatccga tgttttaaga gcgatgctgc catcttcgct aaacttagac
     1441 tgcaataaag cgcctataat catagacgca acaatgatta ctgtgattac tatatacttc
     1501 atactattgt gtactccggc agatcaaacc taaccttaaa gtattgtaac aatctgtcgt
     1561 gattgtagtc agataaatat tcatcaattg agtgaaatta tttagatatt tagacgtgtc
     1621 tttaagatat gaacacacac ccgcattcac actcctcgtt gttcccgata tatctgaaaa
     1681 acatgaatgt gcggggtcta acaacataga taccaggccc aatgaaaatg gatttattat
     1741 tccatttata ggttcatccc atatattttg acttttagaa atcaactatt ccaccaatta
     1801 actaattgat cccacaaacc cggctccggt ggcacgtatg cttccaaaca agatcgccat
     1861 tcttctttgt gggcttcctc ataatttgcc agctgcacat ctgtcatttc catgagcgca
     1921 gccacttctt cacaaggggc gtctgtttta aattttctct taatcatgga cggtaagagg
     1981 ttttcctgat tgttctctcc gtataattct atgtagttca taaaaacgcc ctcaacatat
     2041 ttggcacata agaaataaat gatttcggat agcatatcct tgttacagcg tctatattgt
     2101 aaaaaccggt ccattgatat agaaatgtat ctgccgcagg caagttagaa gatgtactta
     2161 aaacaccact atcgttaccg ccggttaaat ttttgatagc aaaataaaca gtactgcctt
     2221 gtggcgcaca cgctatcata gtggcatacc aagagttacc ggaaaaagca accccagtat
     2281 ctaatctatt ttctgtcgtc ccgtctttag cggaaaacca ccaatttgtt tctgtcaacc
     2341 caccatttac atgactacgc ataaacgcca accgactatt cgttggattg tccgatccga
     2401 gagccgccgc aagcgtcgag gtggcagacg aggtgattcc acttatccaa cgcgtgccag
     2461 ttccgacgcc ggtattatcc caatttgttc caaatggtaa aaaaatatca attaacaaaa
     2521 aaccgttttc accctgagca gacccacgtt ggtacatcgg gttgttattt gtcgcactac
     2581 ggcgtacacc gcctgcggat gctgctgatg tttgcgtaag agtccttaac atacccctgt
     2641 tggtgatagg agcttgagcc gctgcgcctg agtccactgt agttattcca agttgagtat
     2701 tgagactcgc atcggcttcc gcccatcgtg tcgtataaaa cattcgcgat aattcaaagc
     2761 cgccaaaagg cgtttgatct atccaactag ttagccatgt tttattaccg ccaagtaaag
     2821 aacctaacgc atcgccgtca tacttgtaag tagttccagc cccacccccg ctagcctgag
     2881 ccgcgaagaa atgggctaaa gaagaatttg cgcctacatc aggaagttca tctattcttt
     2941 tactcatata ttaaaaaatc tccatctcca gtaacaaagg cgtcactaga ccagtcggtt
     3001 aaggcattag ctggaacggt agaaacttgt ccgccataca tcatttgcca tatagaattg
     3061 tcaacctgac caaacattac aaccaagtac tgggtttaaa taaattaaat gactcactac
     3121 tctgcgaact ttcttgaggt ggcaaatttg acgtcacctc ttcaataata acctcacttg
     3181 gagcgttgat attcgttgtt tctactaccg gttcttgctg ctcttgagtt gctaaattat
     3241 tatctatcgg tagagcatct ccaacaaaga caagttgatc atcgttcttg gcgtaaaaca
     3301 acccttcaaa cacaaaatac tccctaccat caagaagtaa gccagacgga ttaaattcaa
     3361 cttctatgcc accatgaatc ccacctgata ttgtcatatt atacctcgta atagtattta
     3421 acagtagcta taccgcgtat tatttcagaa gccgtagccg taccaacagg catctttaat
     3481 atgatatgaa aaaatgtacc cggctctata attagaggtt caaaattaga atctattctt
     3541 tggttagcca tagcgcctat ggcggtccca acggctatac tttgcgcccc tattgctatc
     3601 ctgcgtggag ccttcccagc ggctgtggca gattctgctg tagctaaaga aaccgccgat
     3661 gacccacctg caacagccca ttgc
//
LOCUS       DAOQLJ010000201         3683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_12976_length_3683_cov_7.587927, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000201 DAOQLJ010000000
VERSION     DAOQLJ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12976_length_3683_cov_7.587927"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PK522_10800"
     CDS             <1..310
                     /locus_tag="PK522_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Appr-1-p processing protein"
                     /protein_id="HNC42260.1"
                     /translation="WSGTGGVHILNLLTQEEALGHQTKPGKASTENVNHALRAGRHII
                     EKEGFRSVALPRLATGVGGLDWNSVMPLIQNHLGDLAIPVYIYAQFIKGKQALEPSNP
                     "
     gene            complement(326..661)
                     /locus_tag="PK522_10805"
     CDS             complement(326..661)
                     /locus_tag="PK522_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HNC42261.1"
                     /translation="MIIEIKTILIFLLTAVAEIVGCYLPYLWLKEGKSIWLLVPAALS
                     LALFAWLLSLHPTAAGRVYAAYGGVYIAVAIFWLWAIDGIKPTIWDIIGACVALIGMA
                     IIMFAPRNT"
     gene            complement(651..1553)
                     /locus_tag="PK522_10810"
     CDS             complement(651..1553)
                     /locus_tag="PK522_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC42262.1"
                     /translation="MNDQWPISFLTSVILLLILVSSSFAQTTISQNLIEFKHKDKIIR
                     NLPLAELERYLTKQTLKIFEVHEKKERTYQVYSATALFDFIFGSDWRAAEEIVFICED
                     QFQPSIPVTKFLQYNAYFAFASADNHPFTLKNLLQNNEKVHLGPLYLVWDNLSSKELQ
                     YEGAVDMPYQIIGIELTTFSARFPAIFPPNDTSAEVQRGFLHFRKHCFACHTINGQGS
                     GKAAELNYPVSVTEYIQPTYLKLWIDNPTAIRLNSKMLSLAADIPDRDQVIDEIIAYL
                     TAMRRVKPQSKTMHIQHNNQSDDN"
     gene            complement(1579..2619)
                     /gene="tsaD"
                     /locus_tag="PK522_10815"
     CDS             complement(1579..2619)
                     /gene="tsaD"
                     /locus_tag="PK522_10815"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNC42263.1"
                     /translation="MIVLGIETSCDETGIALFHTEKGLLSHTLYSQIEMHNEYGGVVP
                     ELASRDHINRVLPLIKQNLAIAQIDLKAINAIAYTQGPGLAGALLVGASIANALAFAL
                     RIPILEIHHLEGHLLSPLLTNPTITFPFVALLVSGGHTQLIRVDKVAHYALLGETLDD
                     AAGEAFDKTAKLLGLGYPGGKSLSLLAERGESGRFTFPRPMRNSGDFNFSFSGLKTSV
                     QTLIKHIEITDQIKADIALAFQEAVVDILVEKSLAALKHCSLSNLVVAGGVGANSQLR
                     QRLTLEASQLGANVHFPELALCTDNGAMIAFAAAMRISSGDKNYLETHSNYRYTVKAR
                     WNLETTAYNHCI"
     gene            complement(2676..3431)
                     /gene="zapD"
                     /locus_tag="PK522_10820"
     CDS             complement(2676..3431)
                     /gene="zapD"
                     /locus_tag="PK522_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HNC42264.1"
                     /translation="MICYEHPLNERVRTLLRLENLFSKVDFFSKCNTPTEHHSALVAL
                     FEILDVTSRADLKSDLLQELEKQRTMLESLRKNPDVSEDALNRVLTDIKVTFRDLLNI
                     PGKIGGHLRDNEWLMSIKQRMSIPGGACEFDLPSYHYWLSLSPEHRHQDFKVWIEPLI
                     PIREGFKIVLMLLRNSGRSYHYTAKQGQFQQTGSDHMAHMIRLRIDENLPCVPEISAN
                     KYALSIRFIPFSPEQKVKVYEDDIEFELTLCNL"
BASE COUNT         1078 a          707 c          782 g         1116 t
ORIGIN      
        1 gtggtcgggt actggtgggg tacatattct caatttgttg acgcaggaag aagcactggg
       61 tcaccaaact aaaccaggca aagcgagcac tgaaaatgta aaccatgcat tgcgagccgg
      121 tcgacatata atcgagaagg aaggttttag aagtgtcgct ttaccgcgtc ttgcaactgg
      181 tgtcggtggt ttggattgga atagcgtaat gccactcatc cagaatcatt taggcgattt
      241 agctattcca gtttatatct atgctcagtt tattaaggga aagcaggcac ttgagccgag
      301 taacccttga tgtgagatag caatttcaag tgtttcgagg ggcaaacata ataatggcca
      361 tgccaattaa tgcaacacaa gccccgatta tgtcccatat cgtgggtttg atgccatcga
      421 tagcccaaag ccagaatatt gcgacagcaa tgtaaacacc cccgtaagcc gcataaactc
      481 ttcctgctgc ggttgggtgc aatgagagaa gccaggcaaa gagggcaaga ctaagggcag
      541 caggaacaag taaccagata ctttttcctt cttttaacca cagatagggt agataacaac
      601 caacaatctc agcaacggca gtgagcaaga agattaagat ggttttgatt tcaattatca
      661 tcagactgat tgttatgctg aatgtgcatc gtttttgatt gaggtttcac tcggcgcatg
      721 gctgttaaat aggcgatgat ttcatcgatg acttgatccc gatcaggaat atcagccgct
      781 aatgacaaca ttttagagtt taatcggatg gccgtaggat tatcaatcca taatttcaaa
      841 taggtgggtt gaatatattc tgtcacactg accggataat tcaattcagc ggcttttcca
      901 ctaccttgac cattgatagt atggcaagcg aaacagtgtt tacggaagtg caagaatcca
      961 cgctgaactt cggctgaagt atcattgggt ggaaaaattg cggggaaacg tgctgagaaa
     1021 gtagtgagtt cgattcctat gatttgataa ggcatatcga cagcaccttc atactgtaat
     1081 tccttagaac ttaaattgtc ccatactagg taaagcggcc ccaggtgaac tttttcatta
     1141 ttttgaagta aattctttaa tgtaaaagga tgattatccg cactggcaaa agcaaaataa
     1201 gcgttgtatt gtagaaattt tgtgactggg atgcttggct ggaactgatc ttcacagatg
     1261 aaaacgattt cttccgcagc tctccagtcg gatccaaaga taaaatcaaa aagtgctgtg
     1321 gctgagtaga cctgatatgt tcgttctttt ttttcgtgaa cttcaaatat ttttaaggtt
     1381 tgcttagtta aatagcgttc aagttccgcc aatggcaagt tccgaattat tttatcttta
     1441 tgcttaaatt cgatcagatt ctggcttata gtagtctgag caaaacttga actgaccagg
     1501 atcaaaagca aaataactga agtcagaaaa ctgattggcc actgatcgtt cattgtttgt
     1561 tgcagtaatc gacaatgctt aaatacaatg attgtatgcg gttgtttcta gattccatcg
     1621 cgctttaact gtatatcgat agttagaatg agtttctagg taatttttat ctccactact
     1681 gattcgcatt gcagccgcga aagcaatcat tgcaccatta tcagtacaca gcgctaattc
     1741 aggaaaatga acatttgcac ctaattgact ggcttcaagc gtgagccttt ggcgcaattg
     1801 tgaattggct ccgactcctc ctgcgacaac gagattactc aggctacaat gcttgagggc
     1861 cgcgagtgat ttttcaacca aaatatcgac gacggcttct tgaaaagcga gggcgatatc
     1921 tgctttgatt tgatcggtta tctcaatatg tttgattagc gtttgaacag atgtctttag
     1981 tccactaaag ctaaaattga aatcgccact gtttcgcatt ggccgaggaa aagtaaatcg
     2041 tccactttct cccctttcag ccagtaatga gagtgatttc cccccagggt atcctaatcc
     2101 taacagtttt gcggttttat caaatgcttc gccagcagca tcatccaatg tttcacctaa
     2161 gagcgcatag tgggcaactt tatcgaccct gattaattgg gtatgacctc ctgatacaag
     2221 tagcgcgaca aaagggaagg taattgttgg attagtgagt aaaggagata gcaaatgtcc
     2281 ttcgagatgg tgaatttcta aaataggaat acgtaaagcg aatgccagcg catttgcaat
     2341 acttgcaccg actagaagcg caccagcaag gcctgggcct tgagtatagg cgatggcatt
     2401 tattgctttg agatcgattt gagcgatcgc taaattttgt tttattaaag gtagaacccg
     2461 attgatatgg tcgcgagaag ctaattctgg gactactcct ccatactcat tatgcatttc
     2521 tatttgagaa tatagtgtgt gacttagtaa gcctttttct gtatggaaaa gagcaatacc
     2581 agtttcgtca catgatgttt ctatccccag aacaatcatt tgctagtttt tgcgtaataa
     2641 aagagaacat cagttaactt gaacgatgat tcgaatcata agttacagag ggttaattcg
     2701 aattcaatat catcttcgta aactttaact ttctgttcgg gtgagaaagg tataaagcga
     2761 atactcaatg catacttatt agcgctaatt tctggtacac aaggcaaatt ttcatcaatt
     2821 cggagtctga tcatgtgtgc catgtgatca gagcctgttt gctgaaattg tccttgctta
     2881 gcggtgtaat ggtaggatct tccactattt ctcagtaaca tcaatacaat tttaaagcct
     2941 tcacggatcg ggataagagg ttctatccat actttaaagt cttgatgacg atgttcgggt
     3001 gataaactta gccaataatg gtaggaaggt aaatcaaact cgcaggcgcc accgggaata
     3061 ctcattcgtt gcttgatact cattaaccat tcattatccc gcagatggcc gcctattttt
     3121 ccaggaatat tgagtagatc tctaaatgtg actttgatat cagttaaaac acgattcaaa
     3181 gcgtcttccg aaacatcagg atttttacgt aaactttcca acatggttct ttgtttttct
     3241 agctcttgga gtagatccga tttaaggtca gctcgacttg taacatccag aatttcgaat
     3301 aatgctacta atgctgaatg atgttcagtc ggtgtattac atttagaaaa gaaatctact
     3361 ttgctgaata aattctcgag ccgcaataat gttcggactc tctcattgag ggggtgttcg
     3421 tagcaaatca cgttaatttt atatgcatca gtttatgaca attcgaatca taccttatga
     3481 tttattatta ctaaatcaaa gttttgtaat ttacgaaaaa aagcttcaac gatctcaaat
     3541 aataataggg aaatgcgttt cgccatccct tttggagggg aaattcccat cggtaatacc
     3601 ctgttacttt ctatggggcg tcattttttt atatgcctct aaactatttt cagtttaatc
     3661 tgggtgtgac tcaaacaggc att
//
LOCUS       DAOQLJ010000202         3656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_13131_length_3656_cov_7.049708, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000202 DAOQLJ010000000
VERSION     DAOQLJ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13131_length_3656_cov_7.049708"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /gene="nadD"
                     /locus_tag="PK522_10825"
     CDS             complement(<1..385)
                     /gene="nadD"
                     /locus_tag="PK522_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNC42265.1"
                     /translation="MKTDYTLIGIYGGTFDPIHYGHLRTAEELVEILQLDGLHFIPAG
                     QPRLRNQPIASRHQRCVMLNKAIRDNSRFELDTREIKGRGESYSVETLRKLNQEYSSR
                     QVAFCFIIGADAFMKLPSWYHWRELF"
     gene            complement(442..1779)
                     /gene="aceF"
                     /locus_tag="PK522_10830"
     CDS             complement(442..1779)
                     /gene="aceF"
                     /locus_tag="PK522_10830"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HNC42266.1"
                     /translation="MGQVKKVLVPDIGDFKEVPVIEVLIHEGERLSIDDALITLESDK
                     ASIEVPSPYAGIVNTIHVKVGDKVSEGAEIITMEVSDAEEKSSTTIQAEAVSDSPAAS
                     NVKQSIAKEIPVAAVEQSEAKSPAQQPSQLSNRSWNANPDITPHAGPSVRRFARELGV
                     DLTRVIGSGPKHRILKEDILAYVKHQLSKPTSSGERGYLDLLPWPHVDFAKFGPIELK
                     TLSRIKQISGANLHRNWVMIPHVTQFDEADITELESLRKEYNESQSANGIKLTILAFL
                     IKATTAALKKFPEFNASLDNSNSEANLVLKRYYHLGFAVDTPNGLVVPVIRDADQKGV
                     ITIAEELGKLSTLARDGKLKPTDMQGASFTISSLGGIGGTAFTPIINAPEVAILGVSK
                     ASLKPVYRNGEFVPRLVVPLSLSYDHRVIDGASAARFTSHLSNILADMRLALF"
     gene            complement(1848..>3656)
                     /gene="aceE"
                     /locus_tag="PK522_10835"
     CDS             complement(1848..>3656)
                     /gene="aceE"
                     /locus_tag="PK522_10835"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNC42267.1"
                     /translation="IKVIWGSYWDPLLAKDTKGLLQQRMMECVDGEYQTFKARDGAYV
                     RKHFFGKYPELLEMVANMSDDDIWRLNRGGHDPHKVYAAYAAAVKHQGQPTVILAKTI
                     KGYGMGESGEAQNITHQQKKMGTTSLRAFRDRFGLPVPDEKIDEIPYLQFDENSEELR
                     YMKSHREALGGFPHHRRRKAQPLNPPALTAFETVLKASGEGHESSTTMAFVRILNILT
                     KDKNIGKHVVPIVADESRTFGMEGMFRQLGIWSSVGQLYTPQDADQLMYYKEDKHGQI
                     LQEGINEAGALSSWIAAATAYSTHGVQMIPFFIFYSMFGFQRVGDLIWAAGDLRCRGF
                     LIGGTAGRTTLNGEGLQHEDGHSHLTASTIPNCVSYDPTFAYELAVIVQDGLRRMYQE
                     QEDIFYYITVMNENYAHPDLPKGAEQDILKGMYLLQDRSHQHKEKKIPLQLLGSGTIL
                     REVMAAAEILQNDYGVAADVWSVTSFNQLRREALSVSRWNMLHPTKNAKTSHIENCLK
                     NRNGPVVAATDYMKLYADQIRAFIPGRYSVLGTDGFGRSDTREKLRHFFEVDRYYITV
                     AALKVLSEEGKIPAKLVSQAIKTLGIDPEKPEPITQ"
BASE COUNT         1006 a          879 c          731 g         1040 t
ORIGIN      
        1 cgaataattc acgccaatgg taccagcttg gtaacttcat aaaagcatca gcgcctatga
       61 taaagcagaa agcaacctgc ctactggaat actcctgatt taattttctt aacgtttcta
      121 cactgtaact ctcacctcgc cccttaattt cccgagtgtc tagctcaaat ctactattat
      181 ctcgaattgc tttattgagc ataacacaac gttgatgccg ggaagcaatg ggttgattgc
      241 ggagccgagg ttgtccagcc ggtataaaat gtaacccatc gagctgtaga atttctacca
      301 gttcttctgc agttcttaaa tgaccataat ggattggatc gaaggtgcca ccataaatac
      361 ctatcaaagt gtagtcggtt ttcactaata tactgctgac aggaaagcca gcgtttgatg
      421 gtggatcgat ctatcaatca ctcaaaaaag cgcaaggcgc atgtcggcaa gaatattcga
      481 caaatgtgag gtaaaacgtg ccgctgatgc tccatcaatc actcggtgat cgtaggacaa
      541 cgaaaggggg acgactaacc gtggtacaaa ttctccattg cgataaactg gcttcaaact
      601 agctttagat acacctagaa tagcaacttc cggagcattg ataatcgggg taaatgccgt
      661 gccaccaatg ccgccaaggc ttgatatcgt gaaactcgca ccttgcatat ccgttggttt
      721 taattttcca tctctagcca gtgtcgaaag cttcccaagc tcttcagcaa tggtgataac
      781 ccctttctga tcggcatcac gaataaccgg aacaaccagc ccattgggcg tatcaaccgc
      841 aaatcctaaa tggtagtagc gctttagtac taaatttgcc tcactattac tattatcgag
      901 ggaagcatta aattcaggga atttctttaa agcagccgta gttgctttta tcaagaaagc
      961 cagtattgtt agcttaatcc cattggccga ttgcgattca ttatattctt tgcggagtga
     1021 ctccaactca gtaatatcgg cttcgtcaaa ctgagtaaca tggggaatca tgacccagtt
     1081 acggtgtaag ttggctcccg atatttgctt gatgcgactt aatgttttta attctatggg
     1141 accaaatttt gcaaaatcaa cgtggggcca gggtaaaaga tctaagtaac cccgttcacc
     1201 tgagcttgtt ggcttcgata attgatgctt gacataagcc aagatatctt ctttaagaat
     1261 tcggtgctta ggcccactcc caatcactcg agttagatcg acccctagtt ctctcgcaaa
     1321 acggcgtact gaagggcctg catggggtgt aatatctgga ttggcattcc atgatcggtt
     1381 agagagctgc gatggttgtt gcgcaggtga tttagcttcg gattgctcaa ctgctgcaac
     1441 cggtatttcc ttagcgatgc tctgctttac attactcgca gcaggcgaat cactcactgc
     1501 ttcagcctgg atggtagtgc tactcttttc ctccgcatcg gatacttcca tggtgatgat
     1561 ttcagcgcct tcagaaacct tatccccaac ttttacatgg attgtattaa cgattccggc
     1621 ataaggggaa ggcacctcaa ttgaggcttt atcggactct agggtgatca aagcatcatc
     1681 aatggataac cgttcaccct cgtgaatcaa cacttcaatg actggaacct ctttaaaatc
     1741 cccaatatct gggactaata ctttttttac ttgccccacg tacgttcctt aatttaatac
     1801 atcactgtct ttctgagata aagactactc atagcgtcac ccatcattta ttgtgttatg
     1861 ggttcaggtt tctcggggtc aatacccaat gttttgatcg cttgagagac caatttagcc
     1921 ggtatttttc cttcctctga caacacttta agtgccgcaa cggtaatata gtagcgatca
     1981 acctcaaaaa aatgtctgag cttctcacgc gtatcggatc gaccaaatcc atcggtaccc
     2041 aacacgctat aacgccctgg aataaaagca cgaatctgat ctgcatatag cttcatatag
     2101 tccgtcgcag caacgacagg tccatttcta ttttttaaac aattttcgat atgcgatgtt
     2161 tttgcatttt tggtcggatg aagcatgttc caacgagaaa cgctcaatgc ttcacgacgc
     2221 aattgattaa aactcgttac actccataca tctgcagcca caccgtagtc attttgaaga
     2281 atttcagcag cggccatcac ttcgcgtagt atcgttcctg aaccgaggag ttgtagcggt
     2341 attttctttt ctttatgctg gtgacttcga tcttgcagca aatacatgcc tttcagaata
     2401 tcttgctcag cccctttagg taaatcaggg tgagcataat tttcattcat gaccgtgata
     2461 tagtagaaaa tgtcttcttg ctcctggtac atgcggcgca atccatcttg aacaataacc
     2521 gctagctcat aagcaaaggt gggatcgtat gaaacgcaat tcgggatagt cgaggccgtc
     2581 aagtgactgt gaccatcttc atgctgcaac ccttcaccat tgagtgtcgt acgcccagcc
     2641 gtaccaccaa tcagaaaacc acggcagcgt aagtcaccag cagcccaaat caaatcaccc
     2701 acccgttgaa aaccaaacat cgagtaaaaa ataaagaaag gaatcatttg cacgccatgg
     2761 gtactataag cagttgccgc agcaatccac gatgacaatg cacctgcctc gttaatgcct
     2821 tcttgcaaaa tttgcccatg cttgtcttcc ttataataca tgagctggtc tgcatcttgc
     2881 ggtgtataaa gttgaccgac cgaagaccaa attcccaatt gtctgaacat gccttccata
     2941 ccaaaggtac gagattcatc agcgacaatc ggtactacgt gctttcctat atttttgtct
     3001 ttagtcaaaa tattgagaat acgcacaaaa gccatcgtgg tcgaagattc atgaccttct
     3061 ccactggctt tcaatactgt ttcaaaggca gtcaatgcag gaggatttaa tggctgcgct
     3121 ttacggcggc gatgatgggg aaatccaccc aatgcttcgc ggtgggattt catatagcgc
     3181 agttcttctg aattttcatc aaattgcaga tacggaatct catcaatttt ttcatctgga
     3241 accggcaaac cgaatcgatc acgaaatgct ctcagagaag ttgtacccat cttcttctgc
     3301 tgatgtgtaa tattttgcgc ttcacctgac tcacccatcc cataaccttt gatggttttt
     3361 gccagaataa cggtcggctg accttgatgt ttgactgctg cggcataagc agcataaacc
     3421 ttgtgaggat catgcccccc acgatttagc cgccaaatat catcatcaga catattagct
     3481 accatttcca gtagctcagg atattttcca aaaaaatgct ttctaacata agcaccatct
     3541 ctggctttaa atgtttgata ctcaccatca acgcattcca tcatacgttg ttgtagcaaa
     3601 cctttagtat ccttagctag caaaggatcc cagtaggagc cccagatcac cttgat
//
LOCUS       DAOQLJ010000203         3651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_13164_length_3651_cov_4.976085, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000203 DAOQLJ010000000
VERSION     DAOQLJ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13164_length_3651_cov_4.976085"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..494)
                     /gene="smbP"
                     /locus_tag="PK522_10840"
     CDS             complement(150..494)
                     /gene="smbP"
                     /locus_tag="PK522_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HNC42268.1"
                     /translation="MKLIRNSIAMGVLAMFLSAPLLAGDAAHTSAAMEHAGEAKAHGE
                     MGHAKELLKHAKESLAHAKAAGKEHADAHKHMDEAIKHLEEAIKHAEMDHAGEAAKHT
                     EEAMKHMRESGH"
     gene            719..2305
                     /locus_tag="PK522_10845"
     CDS             719..2305
                     /locus_tag="PK522_10845"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HNC42269.1"
                     /translation="MTEEQRRQLQQQLWNIANTLRGKMNADEFRDYILGFIFYKYLSE
                     KVERFADSILEQDQLKFAAIDEQTAEGAEIVQAVHDAAVTELGYFLKPSELFHAIAMK
                     GNSQADSDADGEHYNFILPDLTRILKNIEQSTLGTDSADDFSNLFEDLDLTSSKLGAT
                     EKAKNALVTKVLVHLDKIDFSLADGTADVLGDAYEYLIGEFASGAGKKAGEFYTPQPV
                     STLLAKLVTAQNKNIKKLYDPTCGSGSLLLRVKREADHVDKIYGQELNRTTYNLARMN
                     MILHDVHYADFDIRQEDTLEHPQHRELRFDAIVANPPFSAHWSANPLFMSDDRFSVYG
                     KLAPSSKADMAFVQHMVYQLAEDGTMAVVLPHGVLFRGAAEGHIRQYLIAQTNCLDAV
                     IGLPANIFYGTSIPTCVLVFKKCRRNPDNILFIDASQAFDKVKTQNLLRSQHIEKIVE
                     AYLARSDQDKFSHVASLETIKANDYNLNIPRYVDTFEAEAEIDLNAIAEQLRALEVQS
                     QQTDATIAAYCRELGIAPPI"
     gene            2317..2574
                     /locus_tag="PK522_10850"
     CDS             2317..2574
                     /locus_tag="PK522_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42270.1"
                     /translation="MLHINPELELKLKALAEQEHSSPDEFIQRLINHYMQQKQQSELL
                     IHVVHELPEIACFNDQDPLDMQRKLRSEWNLSILDSKNECA"
     gene            2561..>3651
                     /locus_tag="PK522_10855"
     CDS             2561..>3651
                     /locus_tag="PK522_10855"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNC42271.1"
                     /translation="MNVPKLRFKDADGREFPEWGLKELREVCKKIIDGTHFSPKSKEG
                     SRRYLTSKNIRNSGIDLLDCNYISEEEHKEIFKKCPVKMGDILLTKDGANTGNCCINS
                     LQEEFSLLSSVAVIDGMDDLVNNIFLLQVFQSQFGINTINNAMAGQAISRITLEKIKK
                     FSFNFPVITEQTKIANFLTAIDEKITQFTQKCDLLAQYKKGVMQQIFSQKLRFKDNDG
                     REFPEWEENELGKVCNPRQWATISSSQLCKTGYPVYGANGQIGYYHEYNHELETVTVT
                     CRGATCGEVNLISPQSYITGNSMCLDDINLQKLDRRFIYFAMKFRGFKDIISGSAQPQ
                     IVSSAIRKIKFENAYELFSVFFILYSKIN"
BASE COUNT         1071 a          795 c          830 g          955 t
ORIGIN      
        1 acagggacta cccattgagt agacaagtca attaatcaga aactaagttt ctgaattgat
       61 gaggagattg tgcaggaaaa aggaaaatct ccctcagcgt tagagcagtg tggttgctga
      121 gggagtgggt ttataaaacg gtgtggagat tagtggccgg attcacgcat atgtttcatg
      181 gcttcttcgg tatgtttggc tgcttcaccc gcatggtcca tttcggcatg tttgatcgcc
      241 tcttccaaat gcttgatggc ttcatccata tgtttatgcg catccgcatg ttctttgcca
      301 gccgctttgg cgtgcgccaa actttctttg gcatgtttca acagctcttt ggcgtgcccc
      361 atttccccat gcgccttcgc ttctcccgca tgttccatag ctgcagatgt atgcgcggca
      421 tcgcctgcta acagcggcgc gctgagaaac atagccaaca cacccattgc gatactattt
      481 cgtatcaatt tcatcgaaaa ctcctttaac ggttacatga ttgatgacta ctgacattca
      541 ttcttattga cattgattta ctcaactcag ttccatgcta gatattttta aagtgcatcg
      601 caataagtgc taacccttat agacgatggt ggttcaagca gatacactgg tctatttagc
      661 atgtcagcat cggtcgattg gcagtacact atagccattt gaaattacta ccagtcacat
      721 gaccgaagaa cagcgccgac aattgcagca acagctgtgg aacatcgcca ataccctgcg
      781 tggcaagatg aatgccgatg aatttcgtga ttacatcctg gggttcattt tttataaata
      841 cctgtctgag aaagtggagc gttttgcgga ttccatcctg gaacaagatc agttgaaatt
      901 cgcggccatc gatgaacaaa ccgctgaagg cgcagaaatc gtgcaagcgg tgcacgatgc
      961 cgcagtgacg gaactgggtt atttcctcaa gccgtctgaa ttgttccacg ccattgcgat
     1021 gaaaggcaac agccaggccg atagcgatgc ggatggcgaa cactacaatt tcattttgcc
     1081 cgatctgacg cgtatcctga aaaatatcga gcaaagcaca ctcggcaccg acagtgctga
     1141 cgattttagc aatctgtttg aagacctgga tttaacctcg tccaaactgg gtgcaaccga
     1201 gaaagctaaa aatgcactgg tgaccaaagt gctggtgcat ttggacaaaa tcgatttcag
     1261 cttagccgat ggtacggcag acgtgctggg cgatgcctat gaatatctga tcggtgaatt
     1321 cgccagcggc gcgggcaaaa aagcagggga attctacacg ccgcagccgg tttccacctt
     1381 gctggccaaa ctggtaaccg cgcaaaataa gaacatcaaa aaactgtacg atccaacctg
     1441 cggcagtggt tcattattat tgcgcgtcaa acgcgaagcc gatcacgtcg acaagattta
     1501 cggccaggaa ttgaaccgca ccacgtataa cctggcacgc atgaacatga ttttgcacga
     1561 cgtgcattac gccgatttcg acatccgtca ggaagataca ctggaacacc cgcagcatcg
     1621 cgagctgcgc tttgatgcga ttgttgccaa tccaccgttt tccgcgcatt ggagcgccaa
     1681 tccgctgttt atgagcgatg atcgctttag tgtctatggc aagctagcac ccagcagcaa
     1741 agccgatatg gcgttcgtgc aacacatggt ttaccaactt gcggaagatg gcaccatggc
     1801 cgtggtgttg ccgcatggcg tgctattccg tggcgcagcg gaaggccata ttcgccaata
     1861 tttgatcgca caaacgaact gcctggatgc ggtgatcggt ctaccggcca atattttcta
     1921 cggcactagt attcccacct gcgtgctggt tttcaaaaaa tgccgccgca acccggacaa
     1981 tattctgttt attgacgcca gtcaggcatt cgataaagtt aaaacgcaga acctgctgcg
     2041 ctcgcagcat atcgagaaaa tcgtcgaagc ctatctagcg cgtagcgatc aggataagtt
     2101 tagccatgtc gcgagcctgg aaaccatcaa agccaatgac tacaatctca acattccgcg
     2161 ctatgtcgac acctttgaag cggaagccga aatcgacctg aacgcgattg ccgaacaatt
     2221 gcgagcgttg gaagtgcaaa gccagcaaac cgatgccacc attgcggcgt attgtcggga
     2281 attaggcatt gcgccaccca tttaaggagg atttttatgc tacatatcaa cccagaactc
     2341 gaactaaaac taaaagcact cgccgagcag gaacatagtt caccggatga attcatccag
     2401 cgattgatca atcactatat gcagcagaaa caacaatctg agttattgat tcatgttgtg
     2461 catgagctgc ctgaaatagc ttgtttcaac gatcaagacc cgctcgatat gcaaaggaag
     2521 ctgcgcagtg aatggaattt aagtattttg gatagtaaaa atgaatgtgc ctaagttacg
     2581 gtttaaggat gctgatggac gggagtttcc agagtggggg ctaaaagaat tgcgagaagt
     2641 ctgtaagaaa ataatagacg ggacacactt ttcaccaaaa tcaaaagaag gctcaaggag
     2701 atatttaacc tcaaagaata ttagaaactc tggtattgat cttttagatt gcaattatat
     2761 ttcagaggaa gaacataaag agatttttaa aaaatgtcct gtaaaaatgg gggatattct
     2821 attaactaaa gatggtgcaa atacaggtaa ttgctgcatc aatagcttac aagaagaatt
     2881 tagtctttta tcgagtgttg cggttataga tggtatggat gatttggtta acaatatttt
     2941 cttgttacaa gttttccaaa gtcaattcgg tattaatacg attaataatg ccatggctgg
     3001 ccaagcgatt agtagaatta ctctcgaaaa gataaaaaaa tttagcttca actttcctgt
     3061 tataaccgaa caaaccaaaa tcgccaattt cctcacggca atcgatgaaa aaattactca
     3121 gttcacgcaa aaatgtgact tattagcgca atacaaaaaa ggcgtaatgc agcagatttt
     3181 cagccagaag cttaggttta aggataacga tggtcgcgag ttcccggagt gggaagaaaa
     3241 tgaacttggt aaagtttgta atccacggca gtgggcaaca atttcgtcta gccaattgtg
     3301 taagactggt tatcccgttt atggtgcaaa tgggcaaatc ggttattacc atgaatacaa
     3361 tcacgagctt gaaacagtta cagttacatg tcgtggagca acttgtggtg aggttaactt
     3421 aatttcccca caaagttata tcaccggtaa ttcaatgtgt ctcgacgata ttaatctaca
     3481 aaaacttgat agaagattta tttactttgc aatgaaattt agaggattta aagacattat
     3541 atctggttcg gcgcaaccgc aaattgtaag ttccgcaata agaaaaatta aatttgaaaa
     3601 tgcctatgaa ctattctcag tgtttttcat cttatatagc aagatcaata g
//
LOCUS       DAOQLJ010000204         3629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_13305_length_3629_cov_6.670677, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000204 DAOQLJ010000000
VERSION     DAOQLJ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13305_length_3629_cov_6.670677"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PK522_10860"
     CDS             <1..395
                     /locus_tag="PK522_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HNC42272.1"
                     /translation="VSTKFKVLGPNDKIVIEAFDDPDIKGATCYLSRAKTGGISGAVG
                     VAEDTADASIACRQIGPIQLSDEVRNGKKDGDEVFKKSTSLLFKSLQVVRFYDAERNV
                     LIYLAYSDRIIEGSPKNSISIIPIMTWR"
     gene            535..1788
                     /gene="hemA"
                     /locus_tag="PK522_10865"
     CDS             535..1788
                     /gene="hemA"
                     /locus_tag="PK522_10865"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNC42273.1"
                     /translation="MQLFAFGINHNTAPLNIREQIAFPQDVMEHALHDLVEHQAVKEA
                     AIVSTCNRTEIYCSTDKPDAAIHWLTDFHHLPARELDPYLYKFPREQAVKHAFRVASG
                     LDSMVLGEPQILGQMKNAIKSAESAGTLGLLLHKLFQRTFFVAKEVRTSTEIGACSVS
                     MAAAAARLAERIFEEISEQSILFIGAGEMIELCASHFAARSPKRITVANRTVERAESL
                     AKRFNGYPISLSELPEQLALHDIVVTSTASPLPILGKGMLERAIKLRKHRPIFIVDLA
                     VPRDVEPEVAELDDVFLYYVDDLADIVKEGLDARLGAVAQAETIIETNVSDFMRWMAT
                     RELVPTIRALRDQAERFRRHELARASKLLAKGEDPVKVLESFSNGLTNKFLHLPSNAL
                     NHANDQERDQLVELINRLYQLHRPQ"
     gene            1785..2864
                     /gene="prfA"
                     /locus_tag="PK522_10870"
     CDS             1785..2864
                     /gene="prfA"
                     /locus_tag="PK522_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNC42274.1"
                     /translation="MNKSIAERLTRLSVRLEELNQLLSSEKVTTDLDNYRKITRERSE
                     IIPVVELYHAHQQTELDIQTAKEMSADPHLRDFAEAELITGKEKLQQIETELLKQLLP
                     KDPSDERNIFLEIRAGTGGDESALFAGNLFRMYARFAERQGWQVEIISQNPSDVGGYK
                     EIIARIIGYGAYSRLKFESGGHRVQRVPVTETQGRVHTSTCTVAVIPEADEIASVELN
                     PAELRIDTFRASGAGGQHINKTDSAVRITHLPTGIVVECQEGRSQHKNKAQAMSVLAA
                     RILDKQTREQQAEQAATRKSLVGTGERSERIRTYNFPQGRVTDHRINLTLYKMDQIMD
                     GDIGELCSALMAEHQAAQLESLMER"
     gene            2958..>3629
                     /gene="prmC"
                     /locus_tag="PK522_10875"
     CDS             2958..>3629
                     /gene="prmC"
                     /locus_tag="PK522_10875"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNC42275.1"
                     /translation="MPVTIAQLLNDAKYHVSDVDARLLLQHALQVSPVFLAAYSDEFV
                     NAEKMMCFYSLLQRRINGEPIAYIIGQREFYDLTFHVTPAVLIPRPETETLVELALAS
                     IPMDRSYTVLDLGTGSGAIALTLAKHRPLAQVMAIDLSAEALEVAQMNATLLEVDNVH
                     FTLGDWFQGIVGQYDLIVSNPPYVAIGDPHLDQGDLRFEPQIALSAPEDGIACIRTII
                     AQAPQF"
BASE COUNT         1011 a          707 c          885 g         1026 t
ORIGIN      
        1 gcgtatcaac caaattcaaa gtgctaggtc ccaatgataa aatcgtaatc gaagcattcg
       61 atgatcccga tatcaaggga gcaacctgtt atctgagtcg cgccaaaaca gggggtatca
      121 gtggcgctgt cggtgttgca gaagatactg cggatgcctc gattgcttgt cggcaaatag
      181 ggcccattca attatcggat gaagttagaa atggtaagaa agatggggat gaagtattca
      241 agaaaagtac ttcattacta ttcaaatcgt tacaggtcgt tcgtttttat gatgctgaac
      301 gcaatgttct gatttatctt gcctatagcg atcgaattat tgaaggctct ccgaagaata
      361 gcatttcaat cattccgata atgacgtggc gttgataatt tggctttaat cagaagacga
      421 gttagcttgt tagcgactga aagaatttaa atcgtacttt cgtcgcattg ctgacgacaa
      481 aactttcgct ataattacga gatttatatc tttttaacaa cgagcagtat ttccatgcag
      541 ttatttgctt ttggtatcaa tcataataca gcgccactga atatacgtga gcaaattgct
      601 tttcctcaag acgtgatgga gcatgcatta catgatttag tagaacatca agctgtcaaa
      661 gaagctgcca tcgtttcaac ctgtaatcgg acggaaatct actgtagtac tgataagcct
      721 gatgctgcaa ttcattggct gactgatttt caccatttac cagctagaga actcgatcct
      781 tatctttata agtttccgcg tgagcaagct gtaaaacatg cgtttcgtgt cgcgagtgga
      841 ctcgattcca tggtgctagg agagccccag attctcggtc agatgaaaaa tgcgattaaa
      901 tcagcagaga gcgctggaac gcttggattg ctactacata aattgtttca gcgtactttc
      961 tttgttgcga aagaagtgag aacttcgact gagatcggtg catgttctgt ttctatggca
     1021 gcggcggctg cgcgccttgc agagcggatc tttgaagaga tcagtgagca gagcatcttg
     1081 ttcattggag caggtgaaat gatagaactc tgtgcaagtc attttgcagc gcgatcgcct
     1141 aagcgaatca cagtggctaa tcgaacagta gaacgtgcag agagcttagc gaagcgcttc
     1201 aatggctatc cgatttcact cagcgaattg cctgaacaat tagcacttca tgatattgtt
     1261 gtgaccagta ccgctagtcc gttacctatt ttggggaaag ggatgctgga gcgcgcgatt
     1321 aagcttagaa agcaccgacc tatttttatc gttgatttag cagttccgag ggatgttgag
     1381 ccggaggtgg ctgaattaga tgatgtgttt ttatattatg tggatgatct ggcagatatc
     1441 gttaaagagg gccttgatgc aaggcttggt gcggtggcgc aggcggagac aattatcgaa
     1501 accaatgtta gtgactttat gcgttggatg gcgacgcgtg agttggttcc aacgattaga
     1561 gcgcttagag atcaggcaga acgttttcgt cgacatgagc tagcacgagc ctctaaattg
     1621 cttgcaaaag gtgaagaccc agtcaaagtg cttgaatcat ttagtaatgg attaaccaat
     1681 aaatttttgc atcttccttc taatgcgctt aatcatgcga atgatcagga gcgtgatcaa
     1741 ctcgttgagc ttataaaccg gctgtatcag cttcaccgtc cgcaatgaac aaaagtattg
     1801 cagaacgtct tacacgttta agtgtgagac tcgaagaact aaatcaactt ttgagtagtg
     1861 agaaggtaac aacggattta gataattacc ggaaaattac ccgagaacgt tcggagatta
     1921 ttcctgtcgt tgagttatat catgcgcacc aacaaactga gctggatatt caaactgcga
     1981 aggaaatgag tgcagatcct catttacgtg attttgcaga agcggagctt attactggaa
     2041 aggagaagtt acaacagatt gaaacggagt tacttaaaca gcttctccct aaagatccca
     2101 gtgatgagcg caatatcttt cttgaaattc gagcaggaac agggggagat gaatctgcgt
     2161 tgtttgctgg taatttgttc aggatgtatg cacggtttgc agagcggcag ggatggcaag
     2221 ttgaaatcat ttctcagaac ccatcggatg tcggtggtta taaggagatt attgctcgga
     2281 tcatcgggta tggcgcttac tcacggctaa aatttgaatc aggggggcac cgagtgcaac
     2341 gtgttcctgt aacagaaacg caaggtcggg tgcatacttc tacttgtaca gtggctgtga
     2401 ttccagaagc agatgaaatt gccagtgtag aactcaaccc tgccgaatta agaatcgata
     2461 cattccgtgc atctggtgca ggtgggcaac atattaacaa gactgattcg gctgtccgta
     2521 ttactcattt gccaaccggt attgttgttg agtgccaaga aggtcgatcg cagcataaaa
     2581 ataaagcgca ggccatgagt gtacttgcag caagaattct tgataagcaa accagggaac
     2641 agcaagcaga acaggcagcg actcgaaaat cattggtagg aactggggaa cgttcagagc
     2701 gtattcgtac ttataatttt ccccaaggcc gggtaacaga tcaccgtatt aatcttactc
     2761 tctataaaat ggatcaaatt atggatggag atatcgggga attgtgctca gcattgatgg
     2821 ccgaacatca ggctgctcaa ttagagtctt tgatggagcg ttaataatat ctctggtaat
     2881 tgtgaagttc ttgaagtcca ttcatcttgc aattccagtg ttattgatcg attacaactg
     2941 aaataggctg gatgcaaatg ccagtaacga ttgcccagct gcttaacgat gctaaatatc
     3001 atgtaagcga tgtcgatgcg cgattgttgc tgcaacatgc actgcaagta tcccctgttt
     3061 ttttagcagc ctactcagat gagttcgtta atgctgagaa gatgatgtgc ttttactcat
     3121 tgctgcaacg aagaatcaat ggtgagccaa tagcctatat cataggccag cgtgagtttt
     3181 atgatttgac ttttcatgtt acacctgcag ttctcattcc acggcctgaa actgaaacat
     3241 tggttgagct tgctttagct agcatcccaa tggatcgtag ctatactgtg cttgatttgg
     3301 ggacagggag tggagctatt gcgcttaccc ttgctaagca tagacccctg gcgcaagtta
     3361 tggcgattga cttatctgct gaagcactag aggtcgccca aatgaatgca acattgcttg
     3421 aggtcgacaa tgtacatttt accttggggg attggtttca aggtattgtt ggacaatatg
     3481 acttaattgt ttctaatcca ccttatgtag cgattgggga tccacacctt gatcaagggg
     3541 atttacgttt cgagccccag attgcgttgt cagcgcctga agatggtatt gcatgtattc
     3601 gcaccattat tgcgcaagcc cctcaattt
//
LOCUS       DAOQLJ010000205         3615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_13394_length_3615_cov_5.684270, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000205 DAOQLJ010000000
VERSION     DAOQLJ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13394_length_3615_cov_5.684270"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PK522_10880"
     CDS             complement(<1..758)
                     /locus_tag="PK522_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42276.1"
                     /translation="MAFSIEIDATQLSAIFFNIPGITPAQQPTQQNDLEKRIWPMEPG
                     AYKIYVVGQAYDFTLTEQGVIGYDEKLDISNGGYFAGLGTARLQLLGYPVEIIAEQLS
                     SPRIIYPDITPDWVSTKILQSFRLLPGTYGFRPSDSWASVVYQVTQDGKIGYDADLDV
                     NNDGCFAGLGTTRLQLLGYSVEIIAESLSSPRIIYTDVTPDWVSTQIPQLFQLLPGIY
                     RFQPSDSPALVVYQVTPDGKIGYDADLDANNGGC"
     gene            complement(772..1089)
                     /locus_tag="PK522_10885"
     CDS             complement(772..1089)
                     /locus_tag="PK522_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42277.1"
                     /translation="MTFPIRIDVHSLAYANFKISAVTPDWVDIRAVRVWELAEGAHRL
                     LFDSSAVDFTVTAAGKIEYDPALETYVRGFGTPGLEVTGYPVTVDATALADATVLFRL
                     KTA"
     gene            complement(1156..3342)
                     /locus_tag="PK522_10890"
     CDS             complement(1156..3342)
                     /locus_tag="PK522_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42278.1"
                     /translation="MTDNTKRNNPRRNQKTSKETLQSAVAQPSTLVAARGSSKGEIER
                     LNYFTGQFLEATDFKDEQEYHIRMRRFGNRALYSAGILDSGFEVQKISDSEISISSGI
                     GIDAQGRELIIITPLKVGVPIDKKSMTLNITLEYVEKEAETSSDTFVDTEGISVTGRT
                     RIAETPAVEFPEDANITNNQIRIAKITLDANGKIGSIDKAERQFAHARFLGVVGIGQK
                     KAPEDWYRQRAGLEINKGDTNEVALLLSSSGLGWGSGIQFKNTAANGKTYGIYSDSIT
                     GSLHITDEDLKTDRLVIDKNGNVGLGTHNPVAKLQVTGGAIMPAAGNDDASGILFPEN
                     PGTGSGDKAWIRYYARDGEKTALEIGVADNSLGTLQDDILLIPSGGVGIGTREPKAML
                     DVEGGAFLGYESTQSNFNAPLKSGFYQGHGNPQGPEPDTQGDVPDTSHDWSHLIVSRH
                     SNTTNNHQFQIASTYLSNDRIFFRKIADNSPNWNEIATRGVNNFLGNQFIRGSLSVEG
                     EVYIRDHLYINGKIAISSANSANSLVLNFGNIFGNGVSCSGLFEPSSLNVGSYMTSTG
                     KLNPGIGNAYFKGKVMAVELSTTFSDLAENFFSDTMLDPGDVVCFDPIEDRIVKSENP
                     ESTMVCGIISTAPGVLLNSDHGTEKEGNFPLALSGRVPCKVTDENGPIKRGDLLCTSS
                     KSGHAMKAQPVVMSDVALYRPSTILGKALGSLESGSGIIDVLVFLS"
     gene            complement(3361..>3615)
                     /locus_tag="PK522_10895"
     CDS             complement(3361..>3615)
                     /locus_tag="PK522_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42279.1"
                     /translation="LPLEELKDSDHEKEFERRMELAHSVIRLEKPAHTYYQLIPNIIT
                     FRIGQRQEPPPTPSGVQLPTAYKVIVGKNTRLGFARRKKE"
BASE COUNT          933 a          886 c          768 g         1028 t
ORIGIN      
        1 aaacagccgc cgttgttggc atccaaatcc gcgtcgtagc cgattttgcc gtccggcgtc
       61 acctgataaa ccactaatgc cggactgtcc gagggttgaa atctgtatat ccctggcaaa
      121 agctggaaca actgcgggat ctgggtgctc acccaatccg gagtaacatc cgtgtaaata
      181 attctcggac tcgatagcga ctcagcgatg atttcaaccg aataacccag caattgcaat
      241 cgagtagttc cgagccccgc aaagcaacca tcgttattaa catccaaatc cgcgtcgtag
      301 ccgatttttc cgtcctgtgt tacctgataa accacagatg cccaactatc cgagggtcga
      361 aatccgtatg ttcccggtaa aagccggaat gattgaagga ttttagtgct cacccaatcc
      421 ggggtaatgt ctggataaat aatcctcggg ctcgatagct gctcagcgat gatttcaacc
      481 ggataaccca gcaattgcag ccgggcagtt ccaagtccag caaagtagcc accgttgctg
      541 atatccagtt tttcatcgta accgatgacg ccttgttcgg ttagagtaaa atcgtatgcc
      601 tgtccaacca cataaatttt gtaagcacct ggctccatcg gccaaatgcg tttctcgaga
      661 tcgttttgct gagtaggttg ctgtgcaggg gttatgcctg gaatgttaaa gaagattgcg
      721 gaaagctggg ttgcatcgat ctcgattgag aatgccatag ttattcgatg ctcacgcagt
      781 ctttaagcgg aatagaactg tagcgtccgc aagtgcggtt gcatccaccg tgactggata
      841 ccctgtcacc tccaaaccgg gagtcccaaa accccgtaca taggtttcaa gcgcgggatc
      901 gtattcgatt tttcccgccg ccgtgacggt aaaatccacc gcgctgctgt caaacagcaa
      961 acgatgcgca ccctctgcaa gttcccagac tcgtaccgca cggatatcca cccaatccgg
     1021 cgttacagcg gaaatcttga aattggcgta tgcaagcgag tgaacatcga ttcggatggg
     1081 aaaagtcatg gttatgaatg acggcgctgt taacttctcg gttgattgta cgaaattctt
     1141 acatttaaag tctgactaac taagaaaaac cagaacatcg atgattcctg atcctgactc
     1201 caatgaacca agggctttac caagaatcgt gctcgggcga taaagagcta catcactcat
     1261 cacaactggt tgcgctttca tcgcatggcc cgatttggat gatgtgcaaa gtaaatcacc
     1321 tcgttttatt ggtccgtttt catcagtgac tttacaaggt actcgtccac tcaatgccaa
     1381 tggaaaattt ccttcttttt ctgtaccatg gtcagaattt aataaaaccc caggtgcggt
     1441 tgatatgatt ccgcatacca tggttgattc tggattttcc gatttcacaa tgcgatcttc
     1501 gattggatca aagcacacta catctccagg gtctagcatc gtgtcggaga aaaagttttc
     1561 tgccaagtcc gaaaacgtcg tactgagctc cactgccatt accttccctt taaagtatgc
     1621 attgccaatc ccaggattta atttgcctgt actcgtcata taggaaccaa cattcaaact
     1681 gcttggttca aatagtcctg aacaagacac gccatttcca aaaatgttac caaagttcaa
     1741 tactaaacta ttagccgaat tagcggagct aatagcaatt ttaccgttta tatatagatg
     1801 atctcttatg taaacttcac cctcaactga taaagaccct ctgatgaatt gatttcctaa
     1861 aaaattatta acacctcgcg ttgcgatttc attccaatta ggagaattat ccgcaatttt
     1921 ccgaaagaaa attcggtcgt tacttaaata ggtggatgcg atctggaact gatgattatt
     1981 agtagtatta ctatgacgag aaacaatcaa atgtgaccaa tcatgacttg tatcgggcac
     2041 atctccttgc gtatctggct caggtccctg cgggttacca tgaccttggt aaaaacctga
     2101 tttcaaagga gcattaaaat tagactgtgt actctcataa cccaaaaaag ccccaccctc
     2161 aacatccagc atggctttgg gttcacgagt gccaattccc actcctccag aaggtatcag
     2221 taaaatatca tcttgaagtg tgccgagcga attatcagca actccgattt ccaacgcagt
     2281 tttctcacca tctcgggcgt agtatctaat ccaggcttta tctccactcc ctgtgcccgg
     2341 attctcagga aacaatattc cggaagcatc atcattccct gctgctggca taatagctcc
     2401 accagtaacc tgtagctttg caactggatt atgtgtgcca agaccaacat taccattttt
     2461 atcgataaca agccgatcag ttttgagatc ttcatcggtg atatgcaaac ttcccgtgat
     2521 tgagtcagag taaattccat aggttttgcc atttgctgca gtattcttga attgaatacc
     2581 cgatccccag ccaagtccag aggaactcaa gagtaaagca acttcatttg tgtcaccttt
     2641 gttgatctcc aatccagctc tctgacggta ccaatcttcg ggtgctttct tttgtcctat
     2701 gccaactacc cccaaaaacc gtgcatgcgc aaattgacgt tcagctttat cgatactacc
     2761 aatcttaccg ttcgcatcga gagtaatttt ggcaattctt atctgattat ttgtgatatt
     2821 cgcgtcctcg ggaaactcaa ctgccggtgt ttcagcaatg cgggttcttc cggtgacgct
     2881 tatcccctca gtgtccacaa aagtatcgga gcttgtttca gcttcttttt caacgtactc
     2941 gagcgttata ttcagtgtca tcgatttttt atcgattgga actccgactt tcaacggagt
     3001 gataataatc agttcacggc cttgtgcatc aataccgata cccgatgaaa tagagatttc
     3061 tgaatcgctt attttttgaa cctcaaaacc actatccaaa ataccggcgc tgtatagcgc
     3121 gcgattaccg aaccggcgca tgcgaatgtg gtactcctgc tcatccttga aatcggtggc
     3181 ttccaggaat tggccggtaa aataattcag ccgttcgatc tctcctttgc ttgatccccg
     3241 cgcagcaacg agtgttgagg gttgcgcaac agcgctttga agggtttctt tgctggtttt
     3301 ctggttacga cggggattat ttcttttagt gttgtcggtc atgataactc atctccttat
     3361 ctattctttt ttgcggcggg caaatccgag ccgggtgttt ttgcccacga tcactttgta
     3421 tgctgttggc agttggacgc cacttggcgt tggaggaggt tcctggcgct gacctatgcg
     3481 gaaagttata atatttggaa tcagctggta gtatgtatgt gccggttttt ccaggcggat
     3541 caccgagtgg gctagctcca tgcgtcgctc gaattctttt tcatgatcac tgtctttgag
     3601 ttcttcaagc ggcag
//
LOCUS       DAOQLJ010000206         3592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_13524_length_3592_cov_5.069550, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000206 DAOQLJ010000000
VERSION     DAOQLJ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13524_length_3592_cov_5.069550"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /gene="gcvPB"
                     /locus_tag="PK522_10900"
     CDS             complement(<1..847)
                     /gene="gcvPB"
                     /locus_tag="PK522_10900"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNC42280.1"
                     /translation="MLIFEHSRPGRRNYSQAPITEAEKSNIPKNLLRKSPPLLPEVSE
                     MDTVRHYTRLSQKNFSIDTEFYPLGSCTMKYNPRACNSLAMLPQFLARHPLAPEDTGQ
                     GFMACMYELQEILKDVTGMAGVSLTPMAGAQGELIGVMMIRAYHEARGDTKRTEIIIP
                     DAAHGTNPATAVMCGYKVVEIATDKEGDVDMDALKAAVGPQTAGLMLTNPSTLGVFEK
                     NVSEMSRIVHEAGGLLYYDGANLNAVLGKVKPGDMGFDVIHINLHKTFSTPHGGGGPG
                     AAPVGV"
     gene            complement(871..1365)
                     /gene="tpx"
                     /locus_tag="PK522_10905"
     CDS             complement(871..1365)
                     /gene="tpx"
                     /locus_tag="PK522_10905"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNC42281.1"
                     /translation="MVTLGGKPVKIEGVFPKVGDKAPAFSLVNKDLEDTTLASFAGKK
                     KVLNIVPSLDTPVCALSTRKFNQQASNMSNTVVLIIAADLPFAMKRFCETEGLDKVVV
                     LSTMRGSEFMRNYGVAIAEGPLSGITARAVIVLDENDQIVHAELVPEIKNEPNYAAAL
                     AALN"
     gene            complement(1399..2754)
                     /gene="gcvPA"
                     /locus_tag="PK522_10910"
     CDS             complement(1399..2754)
                     /gene="gcvPA"
                     /locus_tag="PK522_10910"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNC42282.1"
                     /translation="MPFIPHTEEDIAEMLAKIGANSIEELFDEIPPALKSGKLTRVPA
                     GLTEMEITRLMLERAQADGFYSNFIGAGAYEHHIPAAVWQITTRGEFYSSYTPYQAEA
                     SQGTLQLLYEFQTMMASLTGMDVSNASLYDGASALAEAALMAVRQHKTSRRILIPKTA
                     HPNYRKVLRAIVSNQQIEVVEVPFCEESGQVLVEQIEQFGQEEFAALVIPQPNFFGVL
                     EQVDALTNWAHQKNALVIAVVNPMSLALLTPPGEWGDRGADIAVGEGQPLGIPLSSGG
                     PYFGFMTCKDELVRQMPGRIIGRTVDLDNKPGFVLTLQAREQHIRRSKATSNICTNQG
                     LMVTAATIYMSLLGPDGLRRIAAQSHANTLSLVEKLEKLPGVKKTFSAPFFHEAAITL
                     PGPVAEILQALKAQHILGGLSLDEFYPGQGNTLLVCATETKTKADLDRYVDVLGQILK
                     K"
     gene            complement(2806..3195)
                     /gene="gcvH"
                     /locus_tag="PK522_10915"
     CDS             complement(2806..3195)
                     /gene="gcvH"
                     /locus_tag="PK522_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNC42283.1"
                     /translation="MSVPSQLKYTTSHEWVKSESDGTVTVGITQHAQELLGDMVFVEL
                     PKVGRKLNQKEECAVVESVKAAADVYSPLAGEVVAANSELESAPEKINQDPYTAWLFK
                     LKLNNAADLEKLLDAKGYQDALDNEAH"
     gene            complement(3227..>3592)
                     /locus_tag="PK522_10920"
     CDS             complement(3227..>3592)
                     /locus_tag="PK522_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNC42284.1"
                     /translation="TTHPLESGLAWTVDLKSERDFIGKQALLNAPNSRQLVGLLLLDK
                     GVLRGHQPVITQHDSAGSTGGEITSGGFSPTLNQSIALARVSSQTAIGETVHVVVRDK
                     QLAARVVKYPFVRNGQALV"
BASE COUNT          969 a          883 c          748 g          992 t
ORIGIN      
        1 ctacacctac cggcgcagca ccaggcccac ctcctccatg cggggttgag aaagtcttat
       61 gtaagttaat atgaataaca tcaaaaccca tatcgccagg cttaaccttt cctaacactg
      121 cattgagatt cgcaccatca tagtaaagta aaccaccggc ctcatgaacg atacggctca
      181 tttcagatac attcttttca aacacaccga gtgtagaagg attagtcagc atcaatcccg
      241 cagtttgtgg tcctactgcc gcttttaatg cgtccatatc aacatcgcct tctttatcag
      301 tggcaatttc cactacttta tagccacaca taaccgcagt ggcaggattg gtgccatgtg
      361 cagcatcagg gataataatt tcagtacgtt ttgtatcgcc acgcgcttca tgataagcac
      421 ggatcatcat cacaccgatc aattcacctt gtgcacctgc cattggcgtt aaactcacgc
      481 ctgccattcc agtaacatct ttgagtattt cttgtaactc atacatacaa gccataaaac
      541 cttgaccagt atcttcaggt gctaatgggt gacgcgcaag aaactgcggt aacatcgcga
      601 gcgaattgca agctctcggg ttatatttca tcgtacaaga gcccaaagga taaaactcgg
      661 tatcaattga gaaattcttt tgagacaacc gagtatagtg gcgtaccgtg tccatttcag
      721 acacctcagg cagcaaggga ggtgatttgc gcagtaaatt tttggggata ttactttttt
      781 cagcttcagt aataggcgct tgggaataat tacgccgccc ggggcgtgaa tgttcaaata
      841 tcagcatgat aagtttttaa gttgagtaaa ttaattgaga gcagccagtg ctgcagcata
      901 gttaggttca ttcttaattt cagggactaa ttcagcatgg acgatttgat cgttctcgtc
      961 caatacgata acagctcgcg ctgtgatccc tgacaaagga ccctccgcga tagcaacacc
     1021 gtaattacgc ataaattccg atccgcgcat ggtagataaa acaactactt tatcgagccc
     1081 ttccgtttca caaaagcgct tcatcgcaaa aggtagatca gctgcaataa tgagtaccac
     1141 cgtattgctc atattgctcg cttgctggtt aaatttacgc gtagacaatg cacatactgg
     1201 tgtatccaag ctgggtacaa tgttcaacac ttttttctta cccgcaaagc tagctaaagt
     1261 cgtgtcttcg agatctttat tgaccaatga aaacgcaggt gctttatcgc ctacctttgg
     1321 aaaaacacct tcgatcttga ccggttttcc acccagtgta accattaccg tgatgtcctt
     1381 atattaaaaa ttactgattc attttttaag aatttggccc agtacatcga cgtatcgatc
     1441 caagtctgct ttggttttgg tctcagtagc acaaaccagc agggtattac cttgcccagg
     1501 gtaaaattca tcgagactca atccacccaa gatatgctgc gctttcagtg cttgtaaaat
     1561 ttcagctact ggccccggta aggtaattgc tgcctcatgg aaaaaaggtg cgctaaatgt
     1621 tttcttaaca ccaggcagtt tctcaagctt ctcaaccagt gataaggtat ttgcatgcga
     1681 ttgcgctgcg atacgacgca gcccatcggg tcccaatagc gacatataaa tcgtcgcagc
     1741 cgtcaccatc aagccttggt tggtacaaat atttgatgtt gctttagaac gccggatatg
     1801 ttgttctcgc gcttgcaatg tcagcacgaa gccaggctta ttatccaaat caacggttcg
     1861 accaatgata cgacctggca tttgacgaac cagctcatcc ttacacgtca tgaaaccgaa
     1921 ataaggccca ccgctagaga gtggaattcc cagtggttgc ccttccccaa cggcaatatc
     1981 agcaccgcga tcgccccatt ctcctggagg cgtcaataat gcaagcgaca tcggatttac
     2041 cactgcaatc accaacgcat tcttttgatg cgcccaattc gttaacgcat caacttgctc
     2101 taacacgcca aaaaagttgg gttgtggaat aaccaacgct gcaaattctt cttggccaaa
     2161 ttgttcgatt tgctcgacca aaacttgccc cgattcttca caaaaaggaa cttctaccac
     2221 ttcgatttgc tgattactga cgatagcacg gagtactttc cggtagttcg gatgcgctgt
     2281 tttgggtatt aaaattctac gagacgtttt atgctgacga acggccatca aagccgcttc
     2341 agccagcgcc gatgcaccat cataaagact cgcgttcgag acatccatgc ccgtcagcga
     2401 agccatcatg gtctggaatt cgtagagtaa ttgcaacgtt ccttggcttg cttctgcctg
     2461 gtatggcgta taagaagaat aaaattctcc tctcgttgta atttgccaaa ctgcggctgg
     2521 gatatggtgc tcataagccc cggcaccaat aaaattcgaa taaaaaccat cggcctgtgc
     2581 acgttcgagc atgagtcgag tgatttccat ttcggtaagg cctgcaggaa ctcgggtgag
     2641 cttacccgat ttcagtgcag gtggaatttc atcaaatagt tcttcaatcg agtttgctcc
     2701 aatcttggcg agcatctcag cgatatcttc ttcagtatgc ggaataaagg gcatgattag
     2761 ttgatcttat aaaaaaataa agtcgtagcc agttgaaaat cgcgattaat gcgcttcatt
     2821 atcaagcgcg tcttggtagc ctttggcatc cagtaacttt tccaagtctg cagcattgtt
     2881 gagtttgagt ttaaatagcc aagccgtgta gggatcttga ttgatctttt ctggtgcaga
     2941 ctcgagttct gaattagcag caacaacctc acctgctagc ggcgaataaa catcagccgc
     3001 cgccttgacg gattcgacca ccgcacattc ttctttttgg tttaatttac gtcctacctt
     3061 aggaagttcc acaaatacca tatcgccgag taactcctgc gcatgctgag taataccgac
     3121 agtcacggtg ccatcgctct cagatttaac ccattcatgt gaagttgtat attttaattg
     3181 tgaaggtacg ctcatttttt actccggaaa aaaattaata ataaaatcaa actaaggctt
     3241 ggccatttcg tacaaacgga tattttacga ctctcgctgc aagctgctta tctctaacaa
     3301 ccacatgcac agtttcgccg atcgctgttt gactggaaac tcgtgctaat gcgatcgatt
     3361 gattcaatgt cggtgaaaaa ccgccactgg taatttcgcc cccagttgat cccgctgaat
     3421 catgttgcgt aatcacgggt tgatggccac gcagcacccc tttatcgagg agtaataggc
     3481 caacgagttg tcgactgtta ggtgcattta acaacgcttg cttgccaatg aaatcccgct
     3541 cacttttgag atctactgtc caggccagcc ccgattcaag cggatgggtc gt
//
LOCUS       DAOQLJ010000207         3571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_13668_length_3571_cov_5.895336, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000207 DAOQLJ010000000
VERSION     DAOQLJ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13668_length_3571_cov_5.895336"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /gene="sucD"
                     /locus_tag="PK522_10925"
     CDS             <1..705
                     /gene="sucD"
                     /locus_tag="PK522_10925"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNC42285.1"
                     /translation="ATVKEAKQATGATVSVIYVPPPFAAAAIDEAVEADLDLVICITE
                     GIPVRDMLRTRYKTQGKKTLLIGPNCPGIITPDEIKIGIMPGYIHKKGRIGVVSRSGT
                     LTYEAVAQLMALGLGQSTCIGIGGDPINGLKHLDVLRLFNDDDETDAVLMVGEIGGTD
                     EEDCARWVKDHMKKPVVGFIAGVTAPPGKRMGHAGAIIAGGKGTAQEKIEVMEACGIR
                     VTCNPAEMGKLLKSVL"
     gene            722..1102
                     /locus_tag="PK522_10930"
     CDS             722..1102
                     /locus_tag="PK522_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42286.1"
                     /translation="MMESVQVFWSRKPYFLSSILLIIFLTSCATTNQLPLPPSKPSTK
                     PSSPVTQPKQAPRPAGPLPPSTRPTYNLSGFPPATKEGYIDGCETAKKTRWGYKDDVR
                     YSNDLQYRMGWDDGHTICGNQPTK"
     gene            1065..2612
                     /gene="dacB"
                     /locus_tag="PK522_10935"
     CDS             1065..2612
                     /gene="dacB"
                     /locus_tag="PK522_10935"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNC42287.1"
                     /translation="MAIPFVAINQRNSRPGIAIVMTMLSDKRINVRHSIKLNLQAFFL
                     IGILSLLSLSLSASELPESVRHALKTAGIPETAVGVYVKEVTVKQPMLTMNPTQALNP
                     ASVMKLVTTYAGLELLGPAFNWKTEFYALGPIEGGVLKGDLAIKGYGDPQLNLENFWL
                     LVRKLRQTGLKEITGNLILDNSYYQFSMGDPGAFDGSPLKTYNVFPEALLVNYRTTAF
                     HITPDPDRNSVRIVTDPISELLDLQNNLKLTRGHCGDWRNQMRVDIRKPEGNGGRVTA
                     TLSGNYSIQCGANIYYMSLHDSAAYIHDFFKKLWVQQGGIFKGSVQQGIVPKNMSPLT
                     VYQSPPLAEIIRGINKFSNNVAARQLYLALGQSLGQSGNAEFVSLDQSNIALRQWLFS
                     KRLSFPELVVENGSGLSRRERISAYHMGELLVAAFHSPVMPEFMASLAISSVDGTMRS
                     RLRGSLVAGKAHMKTGTLNDVSAVAGYVLDHKDRRSVVVFLVNHPNASNARSAMDALI
                     RWVYEKP"
     gene            2660..>3571
                     /locus_tag="PK522_10940"
     CDS             2660..>3571
                     /locus_tag="PK522_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HNC42288.1"
                     /translation="MRIISFHSVIYGLLVGSVLVIANPVSATKMAGTFVSQQTCPAYV
                     SKKKLTNPDESQLTVAGKYTIIEANQKNDPSWYRVIVPNARPKERWIKAECGVFHQEG
                     NGTGGGSAPNQCSTAGHADSYVLALSWQPAFCEIKSNKPECSITDPNVYQARHFTLHG
                     LWPNQRQCGIDYGFCGNVKQKQEDFCDYPSIDLDAETRNNLAEVMPSVSAGSCLERHE
                     WHKHGTCQANWSVQQYYELSADLVRQFNDSGMAYFMNRRIGKVVRTEDFMNRLAAVLG
                     EATRNRIKLICKQDMLVEVQLMLAASLT"
BASE COUNT          977 a          745 c          905 g          944 t
ORIGIN      
        1 gctactgtca aagaagcaaa acaagcaact ggtgccacag tatcggtgat ttatgtcccg
       61 cctccttttg cggctgcagc cattgatgag gctgttgagg ctgatctaga tttggtgatc
      121 tgtattactg aaggtattcc ggtgcgtgat atgctgagaa cccgctataa aacgcaaggg
      181 aagaaaacct tgctcatcgg cccgaattgc ccagggatta ttacgccgga tgaaatcaag
      241 attggtatta tgccgggcta tattcataaa aaggggcgaa tcggtgttgt ttcccgttcg
      301 gggacgctaa cgtatgaagc agtagcccaa ctcatggccc tgggcctggg tcaatcgacc
      361 tgtatcggta tcgggggaga tcccatcaat ggattgaagc atctcgatgt attgaggcta
      421 tttaacgacg atgatgaaac ggatgccgtg ttaatggttg gtgaaattgg tggtactgat
      481 gaagaggatt gtgcacgctg ggtcaaagat catatgaaga aaccggtggt tggtttcatt
      541 gcgggtgtga ccgcaccgcc cggtaaacgg atggggcatg caggtgcaat tattgcaggt
      601 ggtaaaggca cagcccaaga aaagatcgaa gtgatggaag cctgtggtat tcgtgttacc
      661 tgtaatcctg cggagatggg taaattgctt aaatctgttt tatagaggaa cccgcatttt
      721 gatgatggag tctgtgcaag tgttttggag tagaaagcct tactttctat caagcattct
      781 actgataatc tttttgacga gctgcgcaac tacgaatcag ttacctttgc caccgtctaa
      841 gccgagcacc aaaccttcat cacccgttac ccaacccaaa caggcacctc gcccggcagg
      901 gccattgccg ccaagcacgc gaccgactta taacttgagt ggctttcccc cagcaactaa
      961 ggaaggctac atcgatggtt gtgaaacagc aaaaaaaaca cgctggggat acaaagatga
     1021 cgtgcgttat agcaatgatc ttcaataccg gatggggtgg gatgatggcc ataccatttg
     1081 tggcaatcaa ccaacgaaat agtagaccgg gtattgcaat cgtgatgact atgctaagcg
     1141 ataagcgtat caacgtgcgg cactccatca aattgaacct gcaagctttc ttcctcattg
     1201 ggatactaag cttgttgtcg ttatcgctgt cagcttcaga attgccagaa tcagtgcggc
     1261 atgcgttgaa aactgctggg atcccggaaa cagcggttgg ggtttatgta aaagaagtaa
     1321 ctgtaaagca gccaatgctt acaatgaatc cgacgcaagc attgaatccg gcgtctgtga
     1381 tgaaattggt aacgacctat gccgggcttg agttgcttgg tccagctttt aattggaaaa
     1441 ccgagtttta tgcactgggt ccgattgaag gcggtgttct gaaaggggat ctcgcgatca
     1501 agggttatgg agatccacag cttaatttgg aaaatttctg gttgttggtg cgtaaattgc
     1561 gccaaacagg tttaaaggaa ataaccggaa acttaatcct cgacaatagc tattaccagt
     1621 tctcgatggg cgatccgggt gcatttgatg gcagtccgtt aaaaacttat aacgtatttc
     1681 ctgaagcact attggtcaat tatcgtacta ctgcttttca tattacgcct gatcccgatc
     1741 gcaatagcgt acgtattgtg accgatccta tctcggaatt actcgattta cagaataatt
     1801 tgaaactgac gcgtggccat tgcggggatt ggcgtaacca aatgcgcgtc gatattcgta
     1861 aaccagaggg aaatgggggg cgtgttaccg ctacgctgag cggtaattat tcaattcagt
     1921 gtggcgcgaa tatttattat atgagtctgc atgatagtgc agcgtatatc cacgattttt
     1981 tcaagaaact gtgggtccag cagggaggga tttttaaagg ttcggtgcag caagggattg
     2041 tacctaaaaa catgtcacca ctcacggtat atcaatcacc cccattggct gaaattattc
     2101 gtggtatcaa taaattcagc aataatgttg cagcaagaca actttattta gcgctcggac
     2161 aatcactggg gcaatccggt aatgctgaat tcgtatcact ggatcaatca aacattgcgc
     2221 ttagacaatg gcttttttcc aaacgcctta gttttccaga gctcgtagta gaaaatggct
     2281 ctgggttgtc acgccgagaa cgtattagtg cctaccatat gggagaacta ttggtggcag
     2341 catttcatag tccggtaatg cctgagttta tggcatcact cgcgatatca tccgtggatg
     2401 ggacgatgag gagccgatta cgaggttcat tggtagcagg caaagcacat atgaaaaccg
     2461 gtaccttgaa tgatgtatca gcggttgcgg ggtatgtact cgatcataaa gatcgtcgat
     2521 ccgttgtagt attccttgtc aatcatccca atgccagtaa cgcacgatcc gcgatggatg
     2581 cgctaatccg atgggtatat gaaaaacctt aatcgctaag gaatcgtata tcgaaactta
     2641 ttattagtgg agtaactgca tgcgaattat ttcctttcat agcgtaatct acggtttgtt
     2701 ggtggggagt gtactggtta ttgctaaccc cgtatcggct acaaaaatgg cgggcacctt
     2761 tgtgagccaa caaacctgtc ctgcctatgt ttctaaaaag aaactgacca atcctgatga
     2821 aagccagtta actgtagctg gtaaatatac gattatcgaa gctaatcaaa aaaatgatcc
     2881 aagctggtac cgggtaatcg taccgaatgc gcgccctaaa gaacgttgga tcaaagcaga
     2941 atgtggtgtt tttcatcagg aaggaaatgg aacaggtggg ggaagtgctc caaatcaatg
     3001 tagcacggca gggcatgcag atagctacgt gctcgcatta tcctggcagc cagcattttg
     3061 tgaaatcaaa tcgaacaaac ccgaatgtag tattaccgat cctaatgtat atcaagcccg
     3121 ccactttacg ctgcatggtc tttggccaaa tcaacggcaa tgcggcattg attatggctt
     3181 ttgtggaaac gtaaagcaaa agcaagaaga tttctgcgat tatccatcaa ttgacctcga
     3241 tgccgagacc cgtaacaact tggccgaagt catgccaagt gtgtcggccg gttcatgctt
     3301 ggaacgccat gagtggcata agcatgggac ttgtcaggct aactggtctg tgcagcaata
     3361 ctatgagctt tctgctgacc tggtccgtca gtttaacgat tcaggcatgg cttatttcat
     3421 gaatcggcga atcggtaaag tggtgcgtac cgaagatttc atgaatcggc tagcggctgt
     3481 acttggtgaa gctacgcgta accgtatcaa actcatttgt aagcaggata tgttggtaga
     3541 agtgcagtta atgctcgctg ctagcttaac t
//
LOCUS       DAOQLJ010000208         3521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_13965_length_3521_cov_6.707444, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000208 DAOQLJ010000000
VERSION     DAOQLJ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13965_length_3521_cov_6.707444"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PK522_10945"
     CDS             <1..1113
                     /locus_tag="PK522_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNC42289.1"
                     /translation="NIAKAGVIAARAYPNPEVTVMAGPDHGRYPGKDTEGPFSMQRSV
                     TVNQAIENPFMRSSRIGAAEAFVEAGRASFDQARTDLAAQLRVRTYELLLRQEQAALE
                     QNIYSLIEDVRRRIKHNVERGEAARFELVRADTELQSAENRKQAAELTAQRARVALLQ
                     FTAGALPINFEVKGSLKDSIDLPTLEELRKEIPEANPEILRLQAEQERTQLRIDQERA
                     TVLPQLNVLYTNYQDRQFTSNTAGLQVRIPLFYQRRGEIDAAIYDSARIRQTLDYRRY
                     EVSQLFEAAWQALQIAQRRVEMFEGGLIKEAENAVQIAQAAYRFGERGLIEFLDAQRI
                     LRGLQGDFLLARFELQSAAAEIDRLRAHFPKELIPE"
     gene            1110..2279
                     /locus_tag="PK522_10950"
     CDS             1110..2279
                     /locus_tag="PK522_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC42290.1"
                     /translation="MSIKLSVSSMIFFVLLLSGCSLIDSSDETKKEISQQESTEDRDF
                     NYIKVRPELLKRLEIGQPYLVDLADRIQVPSRVQVDEERTAQIGSYVTGRIIDMFVIL
                     GDYVKPGQRLARITSPDLTQSQLAYLRALSRVTVTQKAYDRAQHLLAADVIPLAEVER
                     RKSELEIAQAEYSAAMDQLRLFGMGEGEIKELNKKGKILPWIDIKSTREGYVIARNII
                     IGQVIQPADPLFQVADLSYVWVVGDVPEQVARDVELGQHVEIIVPAISNNPFDGVIIF
                     VSDIVNRLTRTVMTRVMVENPDRKLKPDMMANMHITDPQHKTLVVPEAAVVRELDQDY
                     VFIALSDDYFQRVPVELGPEVSNLRPVLKGISQGHQIVIEGAFHLDSERKLAELE"
     gene            2283..>3521
                     /locus_tag="PK522_10955"
     CDS             2283..>3521
                     /locus_tag="PK522_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC42291.1"
                     /translation="MMAALVRAILKQRLLVLVMGLMLCVAGGFAVKSLSVDAFPDVTN
                     IQVQVATVSIGRSPEEMERLVTVPVEIAMTGLPGLVEMRSLNRSGLSLITLVFTDQTD
                     VFFARQLVMERIIDVTPRMVPGVTPVLGPVSTGLGEVYQYTIGHPNDGNRQLTFEELM
                     ERRTVQDWVVRPLLRSIRGVAEVNSMGGQVKEYQVYVDPNKLRHYDLTLAQVDRALAS
                     NNANASGNILALRYEQYLIRGVGLIASLEDIRNIVLRELDGVPVYVRDVAEVVFGGEV
                     RQGASIKNGDTESVAGIVMMLRGGNAKEIVGRIKEKVKEVNEKGMLPGGLQIVPFYDR
                     TDLVDSALNTVSTTLMESLILVIVVLSVFLGTVRTSFVVCFTLIITPLVTFLVMNHYG
                     MPANLMSLGGLTIALGMMVDP"
BASE COUNT          997 a          634 c          901 g          989 t
ORIGIN      
        1 aatatcgcta aagcaggcgt tatagcagca agggcctatc ccaatccaga agtaacggtc
       61 atggctggtc cagatcacgg aaggtatcca ggaaaagata cagaagggcc gttctcaatg
      121 caacgttcag tgacagttaa tcaagcaatt gagaatccat ttatgcggag ttctcggatt
      181 ggtgcagcag aagcctttgt tgaagcgggg cgtgccagct ttgatcaagc tagaaccgat
      241 ctggcagcac aacttagagt tagaacctat gaactattgc ttcggcagga acaagcagcg
      301 ttggagcaaa acatctatag tttgatcgag gatgtgcgtc gacgcatcaa acataatgtg
      361 gaaagaggag aagctgctcg ttttgagcta gttcgagcag atactgagtt acaaagtgct
      421 gaaaaccgta agcaggctgc agaattgact gcacaaagag cgcgggttgc attgctgcaa
      481 tttacagcag gggcattacc gattaatttc gaagttaaag gttctctaaa agactctatt
      541 gatttaccga cgctagaaga attgagaaaa gaaattcctg aagctaaccc agaaatattg
      601 cgtttacaag cggaacaaga gcgaactcaa ttacggattg atcaagagcg agcgacagtt
      661 ctgccacaat tgaatgtttt gtacactaac tatcaagatc gccaattcac atccaacact
      721 gctggtttgc aggtgaggat tccacttttc tatcaacgac gcggtgagat tgatgcagcg
      781 atctatgatt cggcacgcat ccgccagacc ctggattatc gccgctatga agtaagtcaa
      841 ttatttgaag cggcttggca agcgctacaa attgcccaaa gacgcgttga aatgtttgag
      901 ggaggattga ttaaggaagc agaaaatgca gttcaaatag cacaagctgc ttatcgattt
      961 ggtgaacgag gtttgattga gtttttagat gctcagcgaa tactgcgtgg gcttcagggg
     1021 gattttttat tagcccgctt tgagttgcag tcggctgcag ctgagattga tcgtctgcgt
     1081 gcacattttc ctaaggagtt gattccagaa tgagtatcaa attgtcagtt tcaagcatga
     1141 ttttttttgt actactgtta agtggttgta gtttgattga ctcgagtgat gaaaccaaaa
     1201 aagaaatatc gcagcaagaa tcaacagagg atagagattt taactatata aaagtaaggc
     1261 ctgagttgct taaacgactt gaaattggtc agccctattt agtggatctt gcagacagaa
     1321 ttcaagtgcc tagtcgagta caagtcgatg aagagagaac agcacagatt ggttcctatg
     1381 taaccggtcg tatcatagat atgtttgtaa tattaggtga ttatgtaaaa cctggacaac
     1441 gtttggcacg gataactagt cctgatttaa cacaatcgca actagcttat ctacgcgctt
     1501 tatcgcgagt taccgtcacg cagaaagcgt atgaccgtgc acagcattta ttagcagctg
     1561 atgtgattcc acttgctgaa gtagaacgga ggaaatctga gcttgaaatc gcacaggctg
     1621 agtacagtgc ggctatggat caattaagat tgtttgggat gggggaagga gaaatcaagg
     1681 agcttaataa aaaggggaaa atcttaccct ggatagatat aaaatctacc cgggaaggtt
     1741 atgtgatcgc acgtaatatt attatcgggc aggtaataca acctgccgat cctctgtttc
     1801 aagtagctga tttatcgtat gtatgggttg taggtgatgt gcctgagcaa gtagcgaggg
     1861 atgtcgaatt aggtcagcat gtcgagatta tagttcctgc tatatccaat aatcctttcg
     1921 atggtgtcat catctttgtt tcagatatcg ttaatcgttt gacgcgcacg gtcatgaccc
     1981 gtgtgatggt tgagaaccca gatcgtaagc ttaagccaga tatgatggca aacatgcata
     2041 ttacagatcc acagcataaa actctggtag ttcccgaagc ggccgtcgtt cgcgaattag
     2101 accaggacta tgtttttatt gcattgagtg atgattattt tcagcgagtt cctgtagaac
     2161 tagggcccga agtatcaaat ttacgtccag tactgaaagg aatttcacaa ggccatcaga
     2221 ttgtgatcga aggcgctttt catttagaca gtgagcgcaa gcttgcagaa ttggagtaat
     2281 tcataatggc agctttagtt cgcgcaattc taaaacaacg cttgcttgtt ttagtaatgg
     2341 gtttgatgct atgtgttgct ggtgggtttg cagttaaatc tctatcagta gacgcttttc
     2401 ctgatgtaac gaatattcaa gtgcaagttg ccacggtttc tattggccgc agtcctgaag
     2461 aaatggagcg actcgtaact gttcctgtag agatagccat gacaggactt cctggtctgg
     2521 ttgagatgcg ctccttgaat agaagtggat tatcactaat aacgctggta tttactgatc
     2581 aaactgatgt gttttttgca cgccagttag tgatggaaag aattatcgat gttacacctc
     2641 gtatggttcc tggggtaaca ccggtgctag ggccggtatc aacagggctt ggggaagttt
     2701 atcaatatac gatcgggcac cccaatgatg ggaatcgtca gctaactttt gaagagttga
     2761 tggagcggag aacagttcaa gattgggttg taaggccact gcttcggtca attcgaggtg
     2821 ttgcagaagt taattctatg ggaggacagg ttaaagagta tcaggtttac gttgatccca
     2881 acaaattgcg gcactatgac ttaacacttg ctcaagttga tcgagcgtta gcgagtaata
     2941 acgcgaacgc gagcgggaat attttagcat tgcgttatga gcaatatctc attcggggtg
     3001 tagggctgat tgccagttta gaggatatac gcaatatcgt tctcagagag ttggatggcg
     3061 tgcctgttta tgtccgagat gtcgctgagg tggtttttgg tggtgaagtg cggcaaggcg
     3121 ctagtattaa aaatggcgat accgaatcgg ttgcaggcat cgtaatgatg ctgcgaggag
     3181 gtaatgctaa ggaaattgtt gggcgaatta aagaaaaagt taaggaagta aatgagaagg
     3241 ggatgttacc tggggggtta caaattgttc cgttttatga tcgaaccgat ttagtggata
     3301 gtgcactgaa cacggtttca actacattaa tggaatcact gattctagtt attgtcgtgt
     3361 tatcagtttt tctaggaaca gtgcgcacta gtttcgtcgt gtgttttacc ctaatcatta
     3421 caccgctagt gactttttta gtcatgaatc attatggtat gcctgcaaat cttatgtcgc
     3481 tgggtggatt gaccattgca ttaggaatga tggtggatcc t
//
LOCUS       DAOQLJ010000209         3519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_13979_length_3519_cov_5.960739, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000209 DAOQLJ010000000
VERSION     DAOQLJ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13979_length_3519_cov_5.960739"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1438)
                     /gene="mrdA"
                     /locus_tag="PK522_10960"
     CDS             complement(<1..1438)
                     /gene="mrdA"
                     /locus_tag="PK522_10960"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNC42292.1"
                     /translation="MNQVIELRNHLLELRHFHLRLVVAAIFVVLLFLILFSRFFYLQV
                     LRSDHFTTLAEANRITILPLVPNRGLIYDRHGEILAQNLTTYVLEIVPDKIRNLDSAI
                     DELSKLIEITPVDRRRFEKLRKENTRFKSLPIRTRLSDAEVARFAANRYRFPGMEINA
                     RLLRHYPHAEMVSHVIGYIGRISDKDLEILESNNALENYRGSSHIGRIGIEQSYEKEL
                     HGITGFEQVETDVAGRSVRTLSRTLPIAGSDLILSLDLKLQQAAERAFGEYRGSLIAM
                     DPNTGEILAFVSKPGFDPNLFVDGIDHLNWKLLNESIDKPLNNRALRGVYPPGSTYKP
                     FMALAALELGKRSPDYAINDPGFFSLPGSSHRFRDWRVGGHGRVNLHKSLVVSCDTYY
                     YMLANDLGINAIYNFMGQFGFGKKTEIDIPGEIVGLSPSPEWKMKRFKQNWFPGDTIS
                     IGIGQGYNLATPLQLTFATMILANNGHAF"
     gene            complement(1435..1953)
                     /gene="mreD"
                     /locus_tag="PK522_10965"
     CDS             complement(1435..1953)
                     /gene="mreD"
                     /locus_tag="PK522_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNC42293.1"
                     /translation="MSKDYLEKELPLEAKSRFIFISLVIAFIINLFPLQEIIFLLRPD
                     FVALTLLFWNINQPHHVGMSLAFTLGIIMDVCNSSILGQHALAYCFMSFLAIILHRRL
                     VLFSVFQQAPQIFWILLLTQVIIYISGTLTGSYHSDWYFFLSSVTGALIWPVIALILG
                     ISQKPETDPNAL"
     gene            complement(1965..2846)
                     /gene="mreC"
                     /locus_tag="PK522_10970"
     CDS             complement(1965..2846)
                     /gene="mreC"
                     /locus_tag="PK522_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNC42294.1"
                     /translation="MESVPQFFRTGPSPQARLFFFSLLSILLMIADLRFESLHKVRQA
                     IGVILYPLQKIALAPASFYDQVETLIEDLRLTKEINYLRQQYFLYQQQFSRLQILEEE
                     NTHLRRLLNAAKQTEVKSIMAEILYIPRDPFKRKIILNRGASDGIDAGQVVVDDLGVI
                     GQVTQIFPWSAEVTLITDKDHAVPIQVARNGLRSVISGVGKNNELELRYLSINADIQV
                     NDLLVTSGIGGIYPPGLPVAKVEKIEHDRSYNFSRITCAPIAGVDRNRHVLILSIPPS
                     EPESTTNNNTVESKSSN"
     gene            complement(2956..>3519)
                     /locus_tag="PK522_10975"
     CDS             complement(2956..>3519)
                     /locus_tag="PK522_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNC42295.1"
                     /translation="GSMVVDIGGGTTEVAVISLGGVVYSNSVRVGGDKFDEAIINYIR
                     RNYGMMVGEATAELIKTQIGSAYPGSEVRDIEVKGRNLAEGIPRSFTISSNEIMEALT
                     EPLNNIVSAVKTALEHTPPELGADIAEMGMVMTGGGALLRDIDRLLMEETGLSVVVAD
                     DPLTCVVRGAGIALENIDRSIGIFARD"
BASE COUNT         1061 a          720 c          733 g         1005 t
ORIGIN      
        1 gaaaagcatg gccattattt gcaaggatca tagtggcaaa cgttaattgt agcggggttg
       61 ctaaattgta cccttgccca atcccaatcg atatggtatc acctggaaac caattttgtt
      121 tgaagcgttt cattttccac tcaggggagg gtgataatcc aacaatttca cctggaatat
      181 caatttcagt ttttttacca aatccaaatt gacccatgaa attataaata gcgttaatac
      241 ccaaatcatt tgctagcata tagtaataag tgtcgcagga gacaacgagc gatttgtgca
      301 gattgactct accatgacca ccaactcgcc aatcgcggaa gcgatgtgag ctacccggta
      361 aactaaaaaa accaggatca ttgatggcgt aatctggcga tcgtttacct aattctaatg
      421 cagccagcgc cataaagggt ttataggtag agccgggcgg ataaacgccg cgtaatgcac
      481 gattattcag gggtttatcg attgattcat tcagtagttt ccaattgaga tgatcaatcc
      541 catcaacaaa taaattgggg tcaaatccgg gtttactgac aaatgctaag atttcaccgg
      601 tattgggatc cattgcaatg agggagccac gatattcacc aaaggcacgt tctgctgctt
      661 gttgcagttt taagtccagg gaaaggatta aatcgcttcc agcaatggga agtgttcgcg
      721 ataacgttct gaccgaccta cctgctacat cggtttctac ctgttcaaat ccggtaatgc
      781 catgcaattc tttttcatag ctctgctcaa ttccaattct tccaatatga gaagatccac
      841 gataattttc taatgcatta ttgctttcaa gtatctcaag atctttatcg ctaatacgcc
      901 cgatatagcc gataacatgc gaaaccattt ctgcatgcgg gtagtggcgt agtaagcgtg
      961 cattgatttc cataccggga aaccggtagc ggttggcggc aaaacgtgca acctccgcat
     1021 cagataagcg agttcgaatc ggtagacttt tgaatcgagt attttctttg cgtaattttt
     1081 cgaaacgtcg ccgatcgacg ggtgttattt caatgagctt ggacagttca tcgattgccg
     1141 aatctaaatt gcgtattttg tcaggaacaa tttccaaaac ataggtggtt aaattttgcg
     1201 caagaatctc accatgccga tcataaatta accctctatt gggaaccagc ggtaagatag
     1261 tgatacggtt tgcttcagct agggtggtga aatgatcgct tcgaaggact tgtaggtaaa
     1321 agaaacgcga aaataaaatt aaaaataaca acaccacaaa gatagcagcg acgacaagcc
     1381 ttagatgaaa atgacgtaac tcaagtaaat ggttacgtaa ttcgatgact tgattcataa
     1441 cgcattcgga tctgtttcag gcttctgtga aatacctaat atcaaagcga tgactggcca
     1501 aattaaggcg cctgttacgc tggaaagaaa gaaataccaa tcggagtggt atgaaccggt
     1561 taatgtgcct gaaatgtaga taatcacttg agtaagtagc aatatccaaa agatttgagg
     1621 tgcttgttga aagacactga ataatactaa gcgccggtgt aagatgatcg caagaaatga
     1681 cataaagcaa taagcgagcg catgttgacc taagatgctg ctattgcata catccatgat
     1741 aattcctaaa gtaaaagcaa gactcattcc tacgtggtgg ggctgattaa tattccagaa
     1801 aagtaatgtc agtgcgacaa aatcaggtcg tagtaaaaag atgatttctt gcaacggaaa
     1861 tagattgatg atgaatgcaa tcactaaact aatgaatatg aagcggctct tagcttctag
     1921 cggtaattcc ttctcgagat aatctttgga catgctattt gcctttagtt tgatgatttt
     1981 gattcaacag tattgttatt agtcgttgat tctggttcgg atggcggtat ggaaaggatt
     2041 aatacatgtc gattcctatc gacgcctgca atgggtgcac aagtaattcg agaaaaatta
     2101 taagatcgat cgtgctcaat tttttctact tttgcgacag gtaatcctgg cggataaatc
     2161 cccccaattc ctgacgtgac caacaagtcg ttgacttgta tatccgcatt tatggataaa
     2221 taacgtaact ctaactcgtt gtttttgcca acgccagaaa taaccgaacg caatccattt
     2281 cgcgcaactt ggatgggaac cgcatgatct ttatccgtga ttaaggtgac ttcggcagac
     2341 catggaaaaa tttgggttac ttgtccaatg acacctaaat catcaacgac aacctgacct
     2401 gcatcaattc cgtctgaggc gcctctgttc aaaatgattt ttcttttgaa aggatcacgt
     2461 ggaatataaa gaatttctgc catgatcgac ttaacttcag tttgttttgc agcatttaat
     2521 aatcgcctca aatgtgtatt ttcttcttct aggatttgta gtcgagaaaa ttgctgctga
     2581 taaagaaaat attgttgcct gagataatta atttctttcg ttaaacgtaa atcttcaatg
     2641 agggtttcga cttgatcata gaaactggcc ggcgctaatg caattttttg caacgggtaa
     2701 agaatgacac cgattgcttg cctaacttta tgtaagcttt cgaatcttaa gtcggcaatc
     2761 atgagtaaaa tggatagcaa ggaaaaaaag aacaaacgag cttgtgggct ggggcctgtc
     2821 ctaaaaaact gtggaactga ttccattaat cgccctgttc tagactaaaa aatatattct
     2881 gcgtttgttt gatgtaaaca ccgattttac ttaagttgat gtgacaatgc gttaccatgt
     2941 ttggtgatgc atttactaat cgcgagcaaa aataccaata gagcggtcaa tattttctaa
     3001 tgcaatacca gctccccgaa ccacacaggt taggggatcg tctgctacca cgaccgataa
     3061 tccagtttct tccattaaca atcgatcaat atcacggagt agcgcacctc ctcccgtcat
     3121 gaccataccc atttctgcaa tatcagcacc taattcgggt ggcgtgtgct ctaatgcagt
     3181 ctttacagcg ctgacgatgt tgttgagcgg ttcagttaat gcttccatga tttcattact
     3241 agaaattgtg aagctgcggg gaatcccttc agctaaatta cgtcctttga cttcgatatc
     3301 gcgcacttca gaaccagggt aagcggaacc aatttgcgtt ttaattaatt cagccgttgc
     3361 ttcaccgacc atcatgccat agttgcggcg aatataattg ataatggctt catcaaattt
     3421 atcgccacct acacgaacgg aattagagta aacaacacca cctaaggaaa taacagcgac
     3481 ttcggtcgtt ccaccaccga tgtcgacgac catcgatcc
//
LOCUS       DAOQLJ010000210         3486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_14230_length_3486_cov_5.328767, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000210 DAOQLJ010000000
VERSION     DAOQLJ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14230_length_3486_cov_5.328767"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PK522_10980"
     CDS             <1..194
                     /locus_tag="PK522_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNC42296.1"
                     /translation="RLNQFDPEVVNNSCFNGTAGQGDWCLGGTADIAGGTVTVPEPAT
                     MALLGLGLVGMGLGRRFAR"
     gene            complement(304..1314)
                     /locus_tag="PK522_10985"
     CDS             complement(304..1314)
                     /locus_tag="PK522_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42297.1"
                     /translation="MATRKEEWIMQTKMGTAKKSKSMYDVQTYSNSSLVKKLYFNQLA
                     VPEKTSKATHIPSEQRTSKLESDSDNCLLRRNGYFIREISSLKQRLKVNSLIKRRYAS
                     RGYNTDQTTVFSADKDQITFEACNESGQLGTLTLIIDLGKGLLADQHYQEEIDAFRGQ
                     AKKVCEVSKLAFNPSSSSKEIFASLFHIAYLYAYCIYGVKNAFIEINPRHAAFYKRML
                     GFEQIGEERVCKRVDAPAVLLRLDLEYMREKIIRSQVTSSSNNKSIYGYFLNQNEEHA
                     ILSIIQNLPAQQKKSGQEKLSAAIPLAALNTGNTSTLLYNLFTEAIKENRAIGLEKLV
                     YC"
     gene            complement(1695..2339)
                     /locus_tag="PK522_10990"
     CDS             complement(1695..2339)
                     /locus_tag="PK522_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNC42298.1"
                     /translation="MAIKTTNPPIIPSYWETALKELRERDRILGKLISTDGSEALCSQ
                     GDAFTTLARAIVGQQISVKAAASVWHKLTLQLTEISPYTVFSADTDLLKTCGLSARKV
                     SYIQDLSAKFLEGIHDEAEWQRMDDESLIKLLIQFRGIGRWTAEMFLIFYMLRPNVLP
                     LDDIGLQRAISIHYNQNQTIDKQSIQKIAEEWQPWRSVATWYLWRSLDPIPVAY"
     gene            2605..3054
                     /gene="mraZ"
                     /locus_tag="PK522_10995"
     CDS             2605..3054
                     /gene="mraZ"
                     /locus_tag="PK522_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNC42299.1"
                     /translation="MFRGVTPLSLDSKGRFAVPAKYHDVLKSSCDNRLVITVDSPKCL
                     LIYPQPVWEPIEKRLLTGLSSFNPKIRAMQRLLVGNACDVAMDNAGRLLVVAQLRQFV
                     GLVKDVVLVGQGEKFELWDAEKWNQEIETALEFKDGDMPPELEGFSL"
     gene            3066..>3486
                     /gene="rsmH"
                     /locus_tag="PK522_11000"
     CDS             3066..>3486
                     /gene="rsmH"
                     /locus_tag="PK522_11000"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNC42300.1"
                     /translation="MHLPVLLNEAINALAIKEDGVYVDGTFGYGGHSQSILQHLGQRG
                     RLVAFDKDLSAIDRAQSINDDRFQAIHSSFAKMQEVLHELRIERVDGILLDLGVSSLQ
                     IDEGARGFSFRSNGPLDMRMNTNIGQTAEQWLQSVTET"
BASE COUNT          970 a          598 c          795 g         1123 t
ORIGIN      
        1 gtcgtttgaa tcaatttgat ccggaagtag taaacaatag ctgctttaat ggtacagcag
       61 gtcaaggaga ttggtgtttg ggaggtacgg ctgatattgc aggtggaacg gttacagttc
      121 cagaaccagc aacaatggca ttattaggat tgggtttagt aggaatgggg ttgggaagaa
      181 gatttgcccg ataagaagta aaagaaactg aaaaataaag taacttgcaa ttgattatct
      241 agaagtccgc tctttatgta gtgaggactt ctagattcct aataaaagca tctagattga
      301 gatttagcaa tatacgagtt tctctaaacc tattgctcga ttttctttga tggcttcagt
      361 aaataagtta taaagtaaag tgcttgtatt tccggtattt aatgctgcaa gcggtattgc
      421 tgcggataat ttttcttgcc ctgatttctt ttgctgcgct ggtaaattct gaatgattga
      481 tagtatagcg tgctcttcat tctggtttag aaagtaacca taaatagatt tattattaga
      541 actcgatgtt acttggcttc taataatttt ttctcgcata tattccaagt ctaagcgtaa
      601 caatacagcg ggagcatcaa cacgtttaca cactcgctct tcaccgattt gctcaaatcc
      661 taacattctc ttataaaagg ccgcgtggcg aggattgatt tcgatgaatg cgttctttac
      721 gccgtaaata cagtaagcat ataaataagc gatatgaaac aatgaagcaa aaatctcttt
      781 agaacttgag ctgggattaa aagcaagttt ggatacttcg catacttttt ttgcttggcc
      841 acggaaagca tcaatttctt cctgatagtg ctgatcagca agtaaccctt tgcctaaatc
      901 aatgataagc gttagcgttc ctagctgacc actttcgtta catgcttcaa aggttatttg
      961 atctttatcc gctgagaata ccgttgtttg gtcagtatta tatccacgag aggcatatct
     1021 ccgcttgatg agagagttaa ccttcaggcg ttgcttaagt gatgagattt ctctaataaa
     1081 ataaccattc ctcctgagta gacagttgtc agaatcggat tctaatttag aggttctttg
     1141 ctcagatgga atatgcgttg ctttgctcgt tttttctgga acagccagtt gattgaagta
     1201 tagttttttg actaatgaac tgtttgagta tgtttgtaca tcatacatcg atttagattt
     1261 tttcgcggtt cccatctttg tttgcataat ccattcctct tttcttgtag ccaaatggcc
     1321 tatgaatgta aagcttagct ctgattgatt tcagaagttt ctatatcttt actgtcagcc
     1381 tagatgctta taaaacgagt cttttattct atgtaagctc tcgtaagaag cgctgtatct
     1441 ttgacttacg caataagcgt gccaccaaaa ataattgata atattcaatg ggttgaatga
     1501 tttggtgaat ttctgtctag cttgattgtg tcgctctaag gtaacgcatt atttgggtga
     1561 tattgataac catatgatta aattgattaa attttgtctc taaattgaga cggttgtttt
     1621 ttcaattttg tgtgattgaa aataagaatt gcagagagaa aattctttac ttgctctgag
     1681 aggaattgag tcggttagta agcgactggg atgggatcta ggcttctcca gagataccag
     1741 gtggcgacag accgccaagg ttgccattcc tcagcaatct tttgaattga ttgtttatcg
     1801 attgtttgat tctggttata gtgaatactg atagcgcgct gtaatccgat atcatctaaa
     1861 ggcaacacat tggggcgtag catgtaaaat attaaaaaca tttcagcggt ccatcttccg
     1921 atccctctga attggataag tagtttgatg agcgattcat catccatgcg ttgccactca
     1981 gcttcatcat ggatgccttc gagaaatttt gccgataaat cctgaatata gcttactttt
     2041 cttgcagaaa ggccgcaggt ttttagcaaa tctgtatccg cggaaaatac cgtatatggc
     2101 gagatctcag ttaattgtaa tgtcaactta tgccaaacgc tggccgctgc ttttacagag
     2161 atttgctgcc ccacaatcgc tctagctaag gttgtaaacg catcaccttg agagcacagc
     2221 gcttcgcttc catcggttga aatcagctta ccaagaatcc tatcgcgttc tctcaattct
     2281 ttcagtgcgg tttcccagta actagggatg attggtggat tagttgtttt aattgccatg
     2341 tcaattctga ttaaaggttt cttcccaggc gcgaagtgct gtgagtgtca ttggtaattt
     2401 tactttttat accttctctg cgtaaatcaa attgggtgtg gcgtctgtaa ctcattgtgt
     2461 agattgaaaa gattaattgt ttttgcttga cgtagtattt ttttttaatt agagtggata
     2521 aaagtgggtt attagggtaa ttatgtggta gattgtgggg taaaattaga tcaatgtggg
     2581 cgatgagtat aaaaatggag tgatatgttt cgtggcgtta caccgcttag tcttgatagc
     2641 aaaggaagat ttgctgttcc agcgaaatat catgatgtac tgaaatcaag ctgtgacaat
     2701 cggttagtca taacagtcga ttctcccaaa tgcctactca tttatcccca gccagtatgg
     2761 gaacctattg aaaaacggct attgacaggc ctttcaagtt ttaaccccaa gatccgggct
     2821 atgcagcgac tgcttgtggg aaatgcgtgt gatgtggcaa tggataatgc aggaagacta
     2881 ttggtggttg cgcagctaag acaatttgtt gggttagtaa aagatgttgt gctagtaggg
     2941 cagggagaaa aattcgagct atgggatgct gagaaatgga atcaagaaat tgaaacagcg
     3001 cttgagttta aagatggcga tatgccacca gagttagaag gtttttcttt gtaagcggtg
     3061 cctctgtgca tttgccagta ttgcttaacg aagcgattaa cgcgctggca attaaggaag
     3121 atggcgtcta tgtggatggt acttttggtt acggggggca tagccagtca attcttcagc
     3181 atctaggcca gagaggaaga ttggtcgcgt ttgataagga tttaagcgct atcgatagag
     3241 cacaatcaat caatgatgat cgatttcagg ctatccatag cagctttgca aaaatgcagg
     3301 aagttttgca tgagctaaga atcgagcggg ttgatgggat tttattagat ttaggggttt
     3361 catcgttaca gatcgatgaa ggagcgcgtg gatttagttt ccggagtaat gggcccctag
     3421 atatgcgcat gaatacgaat attgggcaaa ccgcagaaca gtggctgcaa tcggtaaccg
     3481 aaacac
//
LOCUS       DAOQLJ010000211         3464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_14369_length_3464_cov_6.219126, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000211 DAOQLJ010000000
VERSION     DAOQLJ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14369_length_3464_cov_6.219126"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PK522_11005"
     CDS             complement(<1..1241)
                     /locus_tag="PK522_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HNC42301.1"
                     /translation="MQPHAFVAMPFGTKKDSQGNLIDFNRVYRELIQPALRAAGLQPF
                     RADEEHRAGDIRTDMFQELLLADLVIADITIDNPNVWYELGIRHGLRARGVVLICGGE
                     ISTAFDLYTDRKFRYHIKAEGPDPLLVGADIQNLTEMVKATMQSWHGRKTSPVYQLLP
                     HLQEPDWKSLRIGGIQEYWEKYQAWENRIKLAVNAGYLGDVLVLADEAPVAALRAEAW
                     IKAGKALRQHEHFHFALDQLERGLAIEPDNLLGLHEKGICLQRLAQRNESGYSLDRAR
                     THYQSILSQESYQHDAETWALLGRIDKDAWYSAWYQPNQTTQSYRDEAAYQSPLLKNA
                     IESYRTAYRSNPSHYYSGINALTLMYLYEDLTHDTRYHAEMSAMAGAIRFAACVETET
                     HPSFWPQATLGDLEILVGTPET"
     gene            1818..3260
                     /locus_tag="PK522_11010"
     CDS             1818..3260
                     /locus_tag="PK522_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNC42302.1"
                     /translation="MFSIRCIESTIRIMLPAAFLLVLTPLSADDKHGHANQLPNMFTS
                     TNAHGKDATYSTAGFIDLNNPFFKNLGSNGRSCASCHIPSQGWTITPQGVQAIFDKTA
                     GLDPIFRLVDGANSPTADVSTVEERREAYSMLLNKGNIRVGIGIPADAEFILVEADDP
                     YGFANENELSLFRRPMPTTNLKFLSTVMWDGRETTRDANSTDCIYGTAVCFSPVSFDL
                     STQSNHATLGHAEALRELTQEERDAIVAFELELFTAQIHDKKAGELTAEKGRGGPKKL
                     VKQDYYFGINDTLVGDYKTLAPFNSEVMSLYDSWNRYAPGNSHQVKSARAAIARGEDL
                     FNSKPIQITGVSGINDDLGVAVLPGTCTTCHNTPNSGNHSTPMPLNIGIADESRRTPD
                     MPLYTLQNKATGEIVKSTDPGRALITGKWKDIGRFKGPILRAVASRPPYFHDGSAANL
                     AEVVEFYNNRFSMGLTTQEKADLVAFLAAL"
BASE COUNT          936 a          728 c          815 g          985 t
ORIGIN      
        1 gtttctgggg tacccaccaa tatttccagg tcgccgagcg tggcttgagg ccagaatgaa
       61 gggtgggttt ccgtttcaac gcaagctgca aagcggatcg caccggccat ggcgctcatt
      121 tcggcgtgat atcgagtatc gtgagtgaga tcttcgtata aatacatcag ggttagcgca
      181 ttaattcccg agtagtaatg gctggggtta gagcggtatg cagtgcgata gctttcgata
      241 gcgtttttta gtaatggcga ttggtaagcg gcttcgtcgc ggtaactttg tgtcgtttga
      301 ttgggctgat accaggcgct ataccacgca tctttgtcga tccgacccag caatgcccaa
      361 gtttcagcgt cgtgctgata gctttcctgg ctgaggatgc tttggtaatg cgtgcgcgcc
      421 cgatctaaag aatagccaga ttcgttgcgc tgcgctaagc gttgcaaaca aatgcctttt
      481 tcgtgtaacc ccagtagatt atccggttca atggcaaggc cacgttcgag ttgatcgagc
      541 gcgaagtgga aatgctcatg ttgacgaagc gctttgcccg ctttaatcca ggcttctgca
      601 cgtaatgccg cgacaggcgc ttcatctgcg agcaccaaca catcccccag gtatccagcg
      661 ttgactgcaa gcttgattcg attttcccaa gcttggtatt tctcccagta ttcttggata
      721 ccgccaattc gcagcgattt ccaatccggt tcctgaagat ggggcagtag ttgataaaca
      781 gggctggttt tgcggccatg ccatgattgc atggtagctt tgaccatttc ggtcaagttt
      841 tgaatatcag caccgactaa caggggatct gggccttccg ctttgatgtg gtagcgaaat
      901 ttgcgatccg tatagagatc aaacgcagtg gaaatctcac caccacaaat taacaccaca
      961 ccgcgtgccc gcaggccatg ccgtatccct agctcgtacc aaacattggg attgtcgatc
     1021 gtgatatccg cgatgacgag atcggccagt aaaagctctt gaaacatatc cgtgcgaata
     1081 tcaccagcac gatgctcttc atcggctctg aatggctgta agccagcagc gcgcagtgct
     1141 ggttggatga gttcacgata gacacgattg aaatcgatta gattaccttg gctatctttt
     1201 ttggtaccaa agggcattgc gacaaacgca tgaggttgca tggctggcct ctcttaaatg
     1261 ataggggtgg tgatagttta ctggaaggaa aggaatcgtt aaacgcaaca gaaatagaaa
     1321 gattgaaaac atctctactc gggtcagttt ccaagtatgt attatgcgct tcgtgttgtt
     1381 tcaatgtgtc ttgtaatctt ttataaaaat caattttata ttggtattcg tcctgtacta
     1441 aggggccaat agatgaagca agcgattttc gacagaattc ggcgtgttgg ttactgatga
     1501 tagtcaatct ttatgtaagc aacttaaagg gttcttacaa aatctgcgca tgtgtttttt
     1561 cacacagacc ctatgaatcc gaattagtaa gatttttatc ggcagtgggc aaattagaaa
     1621 ttgaatttgt tttcgatgcc tacagttgat gtatcttatg actgaaaatt aattcttata
     1681 agggaagtga aagcagactc aagtgataag tgtaatcaag tgatgcaata aataaatcaa
     1741 cttgctgttc tcaaaataat taggtagtaa atctaggtaa ttttcttaaa ctaatagaca
     1801 aataaacagg agatcaaatg tttagtattc gttgtattga atcgacaata cgaattatgt
     1861 taccggctgc attcttgctg gtgttaacgc cgctgtcagc agatgataag catggacacg
     1921 ctaatcaatt acctaatatg tttacctcga cgaatgcgca tggaaaagat gcgacataca
     1981 gtactgcagg ttttattgat ctcaataatc cattttttaa aaacttgggg agtaacggac
     2041 gtagctgtgc ttcatgccat attccttctc agggatggac tattacccct cagggcgtgc
     2101 aagcaatttt tgataaaacg gctggactcg atcccatttt tcgtctggta gatggtgcta
     2161 attctcccac ggcggatgtt tcgacggttg aagaaagacg cgaagcctat agcatgctgc
     2221 tcaacaaggg aaatattcgg gtagggattg gtattccggc tgacgccgag tttattttag
     2281 tcgaagccga tgacccctat ggctttgcaa acgagaatga actttcactg ttccgtagac
     2341 ccatgccaac cactaatctt aaatttttaa gcacagtgat gtgggatggt cgtgaaacga
     2401 cccgagatgc taattcaact gactgtatct atgggacagc ggtttgtttt tcaccggtat
     2461 cgtttgattt atctactcaa tcgaatcacg ctacattagg gcatgcagaa gcgctaagag
     2521 aactaacgca agaagagcgt gatgccatcg tggcatttga actggagtta tttacagcac
     2581 agatccatga caagaaagct ggggagctaa cagcagaaaa gggacgaggt gggccaaaga
     2641 aattagttaa acaagattac tactttggta tcaacgatac gctggtcggt gattataaaa
     2701 cccttgcacc ttttaattca gaggtgatgt cgctttatga tagctggaat cgttatgcgc
     2761 cgggtaacag tcatcaagtt aaatctgccc gcgcagccat tgcacgaggt gaagatttat
     2821 tcaacagcaa accgatccaa attaccggtg tcagtggcat taatgatgat ctaggcgtag
     2881 cagtattgcc gggtacttgc actacttgcc ataatacccc caattctgga aaccactcga
     2941 caccgatgcc attaaatatt ggtatcgccg atgaatcacg gcgtactccc gacatgcctt
     3001 tatatacgtt acaaaataaa gcgacgggtg aaatcgttaa atcaacagat cctggccgtg
     3061 cgttgattac cgggaagtgg aaagatattg gtcgttttaa aggaccaatc ctacgcgctg
     3121 ttgcatcacg cccaccttat tttcatgatg gttctgctgc taatttagca gaagttgtgg
     3181 agttttataa caatcgcttt agtatgggtt taacaactca agagaaagca gacctcgttg
     3241 cattcttggc tgcgttgtaa ttcttatcac tcgttattac tggatacgct agttcatagg
     3301 gtatccagga tgcttgatac gatcaggtga tcgtatgtac tagctaacaa gtcgttcctg
     3361 aatctctgaa gagagctcat ttattttttt attacgctat ccgctcaaga aatagcgctt
     3421 cctgcaattc atactacttt ctcttgtaaa gtatcatgta ccaa
//
LOCUS       DAOQLJ010000212         3451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_14469_length_3451_cov_5.695819, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000212 DAOQLJ010000000
VERSION     DAOQLJ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14469_length_3451_cov_5.695819"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PK522_11015"
     CDS             complement(<1..399)
                     /locus_tag="PK522_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42303.1"
                     /translation="MEKRGRKSSEENYQQGFMLQSCYLRLEPDEALSMAEKVIWEEVI
                     SNVPNNFFTPMHKPLLEMYCKHVAQSRLLNKELHTLNTDLLKTREGLRHYDKLLRMRE
                     RENKMASSFATRLRITKQAVHPATAFRALAN"
     gene            complement(600..875)
                     /locus_tag="PK522_11020"
     CDS             complement(600..875)
                     /locus_tag="PK522_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42304.1"
                     /translation="MSILAELIKGKKTYDTIPDEAVTEFNNEVTCSITDNSPSIEAVR
                     AWLYKIEEPEEYHHIVLDKCKADDEALAYFLKHANGEFEVKDLLNKN"
     gene            complement(995..2572)
                     /locus_tag="PK522_11025"
     CDS             complement(995..2572)
                     /locus_tag="PK522_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HNC42305.1"
                     /translation="MIENSISPSESFSNNLANVANTGELNLDDAVDPPINSDWPQPQP
                     ITARIQSEPYPIDALPDVIRRAVEEVGAFVKAPTVLVASSALGSLSLACQAHVDAKRA
                     EKLQGPTGLFLLTIADSGERKTTCDSFFTSAIRQYQEEQAEAMKITVKEYESENAAWL
                     AEREGLLSAIKEAGKKSKSTEVFKENLKQLEYSKPEPLRIPRLLYVDTTPEALAYNLA
                     KQWPSGGMISSEAGIVFGSHAMGKDSIMKNLSQLNQLWDGNSLAIDRRTSESFIVKGA
                     RLTVALQIQETTLKSFFNKSGELARGTGFLARFLVAWPESTQGSRMFTEAPQSWPYLS
                     EFNRRITAILNDPVPIDENGALTPTMLELSPQAKQAWVSYHDTIEKQLANGKELYDVR
                     DVASKSADNAVRLAALFQWLNKPNQAISLDAFDSASRIVSWYLSESRRFFGELALPEE
                     LANAAKLDSWLIDYCKREQTHLVAKNAVLQYGPLRKKEKLDAATQELAELDRLQVQKD
                     GKRIILVINPNLLDFNN"
     gene            complement(2573..3424)
                     /locus_tag="PK522_11030"
     CDS             complement(2573..3424)
                     /locus_tag="PK522_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNC42306.1"
                     /translation="MLNLSEVVKRQFRQAMQAAGIAYSDEIIPDGQLHRFHIEGHKKG
                     SLNGSYRLHIDNRPAGYFQDFKSGISQKWRSSGNSYVSTDVIKSFKEANHQREIEARQ
                     KQEEAAKKAAEIWQKSKPVNNQVDHPYLVKKAVQPYGARLYCNALAISIYNEGNRLVN
                     LQFIDPEGCKRFLSGGRKHGCFYIIGDLSPRILIAEGFATGASLFKSCGQRVVIAFDA
                     GNLLPVAKNIRELSPDSEIIICGDNDLSGIGQAKAREAAIAIGGKVLIPPRPGMDWND
                     YLTMEKK"
BASE COUNT          918 a          771 c          655 g         1107 t
ORIGIN      
        1 attagctaat gcacgaaatg cagtcgctgg atgaactgct tgctttgtaa tacgaagacg
       61 tgtcgcaaaa gaagaggcca ttttattttc acgctcacgc attctaagta atttgtcata
      121 atgacgtaac ccctctcttg ttttgagcag gtctgtgttc aatgtatgaa gttccttatt
      181 caagagccta ctttgtgcaa catgcttaca atacatttcc aataaaggtt tgtgcattgg
      241 cgtaaagaaa ttatttggta cattactgat tacttcttcc cagatcactt tttccgccat
      301 acttaaagcc tcatcaggct ctagccttaa atagcaagat tgcagcatga atccttgctg
      361 ataattttct tcactcgatt ttctacctcg tttttccatt ttttctccac atttagctaa
      421 aaagagctag gcgcgcggtc tacaacacct tttccgttaa agttttaatg ccccccctgt
      481 tgactatatt tacactacct ctcaaaacaa atcaggaagc gttttaagca attaaggctc
      541 attttgatac ctgcccatta atgccattat attaacgcaa cactgttgca tttaaagatt
      601 caatttttat taagtaaatc tttaacctca aattcaccat tagcatgctt taagaaatag
      661 gccaaagcct cgtcatcggc cttacattta tccaagacga tatggtgata ttcttctggc
      721 tcttcgattt tatataacca ggcgcgaaca gcttcaatcg atggtgaatt atccgtaatg
      781 ctgcaagtaa cctcattatt gaattcagtt acagcctcat caggaattgt gtcgtaggtt
      841 ttcttacctt tgattagctc tgcaagaatg ctcataacat tacctcattt tttagcttgg
      901 ttagtttgct actgctattc ttgctattgt tgctattttc agcattaaac ttttctatct
      961 gtagcaggaa tagcaactgt agcaatagca aatgctaatt attaaaatct aacaggttag
     1021 gattgattac taagattatc cgcttaccat ccttttgcac ttgcaatcga tcaagttcag
     1081 caagctcctg agttgccgca tcaagttttt cctttttacg gagtggccca tattgcaata
     1141 ctgcgttctt tgctactagg tgcgtttgct cccgcttaca ataatcaatc aaccagctat
     1201 cgagttttgc tgcatttgct agctcttctg gtagcgccaa ttcaccaaaa aagcgtcgtg
     1261 attcagataa ataccacgac acaatacgac tagcactatc gaaagcatca agacttattg
     1321 cctggtttgg tttgtttaac cattgaaata atgcggctaa ccgtactgca ttatcagctg
     1381 atttgctggc tacatcgcgc acgtcatata actctttacc attggctagc tgtttttcta
     1441 tcgtgtcgtg atagctaacc catgcttgct ttgcttgagg tgatagttca agcatagtcg
     1501 gtgttaatgc gccattttca tcaatcggaa caggatcatt taagattgcg gtaattcgtc
     1561 gattgaattc ggaaaggtaa ggccagcttt gaggcgcttc ggtaaacatc ctgctacctt
     1621 gcgttgattc aggccatgcc accagaaaac gagcaagaaa accagtgcca cgggctaatt
     1681 caccagattt attaaagaat gacttgagcg ttgtttcctg aatttgcaaa gcgacagtaa
     1741 gacgcgctcc ttttacgata aaggactcag aagttcttcg gtcgattgct aggctattac
     1801 catcccataa ttgattgagt tggctaaggt ttttcataat cgaatccttc cccatcgcat
     1861 gcgaaccaaa gacaatgcct gcctcacttg agatcattcc ccccgaaggc cattgcttag
     1921 ccagattgta ggctaacgct tcgggtgttg tatcaacata gagaaggcgt ggtattctta
     1981 gcggctccgg tttgctatat tcaagttgct tcagattttc cttaaagact tccgttgatt
     2041 tgcttttctt acctgcttct tttatagctg acagcaatcc ttctctttct gctagccatg
     2101 ctgcattctc agattcatat tctttgactg ttattttcat agcttccgct tgttcctctt
     2161 ggtactgacg aatggcagaa gtaaaaaagc tatcgcaggt agttttacgc tcacctgaat
     2221 ctgcaatcgt tagtagaaat aatccagtag gcccttgcag tttttcagct cgtttagcgt
     2281 caacgtgggc ttggcatgct agcgacagtg agcccaatgc acttgatgct actagcacgg
     2341 tcggagcctt aacgaatgct ccaacttctt ctacagcgcg tctgataaca tctggtaacg
     2401 catcgatagg atagggttct gattgaattc ttgcggtaat tggttgaggt tgaggccaat
     2461 cactgttaat tgggggatca actgcatcat caagattcaa ttcgccagta ttggcaacat
     2521 tggcaaggtt attcgaaaag ctttcagatg gagaaataga attttcaatc atttattttt
     2581 tctccattgt taaataatca ttccaatcca taccaggtct tggtggtatt agcaccttgc
     2641 caccaatagc aattgctgct tctcttgcct tagcctgtcc aatgccagac aaatcgttat
     2701 ccccgcatat gatgatttct gaatctggcg ataattctcg gatattcttt gctaccggca
     2761 ataggtttcc agcatcaaaa gcaatgacaa cacgctgtcc gcaactcttg aataaactgg
     2821 ctcctgtagc aaatccttca gcaatcagaa tacgtggcga cagatcgcca ataatgtaaa
     2881 aacagccgtg cttacgtcca cccgaaagaa aacgtttgca gccttcagga tcgataaatt
     2941 gcaaattaac cagtcgatta ccttcgttgt aaattgagat agcgagtgca ttacaataaa
     3001 gtctagctcc gtaaggttga acagcctttt tcaccaaata aggatggtct acttgattgt
     3061 tgactggttt ggatttctgc cagatttcag cagccttttt agctgcttct tcctgctttt
     3121 ggcgtgcttc tatctcgcgc tgatgattag cttctttgaa gctcttaatt acatcagtgg
     3181 aaacatatga atttcctgag ctacgccatt tctgagaaat accacttttg aagtcttgga
     3241 aataacctgc aggccggtta tctatatgaa gtctataaga tccgtttaag ctaccttttt
     3301 tatgaccttc gatatgaaat cgatgcaact gcccatcagg gataatttca tcagaataag
     3361 ctatcccggc cgcctgcata gcctggcgga attgtctctt tactacttca gataaattaa
     3421 gcacgatagc ttcccgtacg gttagctaaa g
//
LOCUS       DAOQLJ010000213         3449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_14484_length_3449_cov_6.160283, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000213 DAOQLJ010000000
VERSION     DAOQLJ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14484_length_3449_cov_6.160283"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="PK522_11035"
     CDS             <1..170
                     /locus_tag="PK522_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNC42307.1"
                     /translation="HIAIFAGIGDRIEITHKASNRKTFALGALRAAQFLMRQEKGLFD
                     MQDVLGLRQGL"
     gene            188..1027
                     /locus_tag="PK522_11040"
     CDS             188..1027
                     /locus_tag="PK522_11040"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HNC42308.1"
                     /translation="MATYAIGDLQGCFKQLQHLLEFIAFSPSRDRLWFVGDIVNRGPD
                     SLSLLRFISQLGDSATIVLGNHDLHLLLVAEEITSQRGGDTLQAILNAPDRNQLLYWL
                     RHQKMLHVEDDYVLVHAGLLPSWPASFALELAQEVEATLQSNDYTDLLLHLYGNQPDQ
                     WHDDLKSYDRLRLIVNALTRLRICTKEGKMDFSYKGPLETIPEGYFPWFDVPSRQSQK
                     STIIFGHWSAIRLQLRHNLIALDTGCLWGAELTAIRLEDRKIFQIPCVQPVKRAGVKS
                     LVR"
     gene            complement(936..1613)
                     /locus_tag="PK522_11045"
     CDS             complement(936..1613)
                     /locus_tag="PK522_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC42309.1"
                     /translation="MAKKWAQRFLRILNIKLHYHGSLPSDDQARVILAANHISWLDVI
                     AILAIYPVRFVAKSEILDWPIIGKLSANAGTIFVAREKRNDTLRVNQFLSEILIAGDR
                     IAIFPEGTTSDGTHLLHFHASLLQFAVASKGLVYPVAIHYRDAMNQPTTLTAYVDVSI
                     TQSLQLILQQPSCHVELTFLDPLQGEEKNRRELARLTEQAISHQLALPVVRKGSEKSS
                     DLPSESQ"
     gene            complement(1852..2787)
                     /locus_tag="PK522_11050"
     CDS             complement(1852..2787)
                     /locus_tag="PK522_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNC42310.1"
                     /translation="MRTLICGSIAYDTIMVFQDHFKHHILPEKIHVLNVAFLVPEMRR
                     EFGGCAANIAYNLKMLGEDPLIMATVGDDFQPYAYRCEKVGFDKTHIRQIDDTFTAQA
                     FITTDLDDNQITAFHPGAMNFSHHNSVKEAKHVSLGIVAPDGRDGMIAHAREFQECGI
                     PFIFDPGQGLPMFSGDELVDFISKADYVTVNDYEAELLLERTGYSLGKLASLVKALIV
                     TKGAQGSVIYTGGHHIEIPSVQPKAIVDPTGCGDAYRAGLLYGIAHGLDWYATGKLAS
                     LLGALKIAYRGGQNHEFDRSAIDQHYFEAYGNRIF"
     gene            complement(2876..>3449)
                     /locus_tag="PK522_11055"
     CDS             complement(2876..>3449)
                     /locus_tag="PK522_11055"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111236.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNC42311.1"
                     /translation="VAKTEKGDYRWLTEKDLPKSIGRLSAHMGNAGVLLRAYIYVRLL
                     GFDGMHRISEFATLNANYLMAELREVGFEIAYPTRRASHEFIVTLKEIKDRTGVTAMN
                     LAKRLLDKGYHAPTTYFPILVPECLLIEPAETESKETLDAFVAAMKEILVEIKEQPDM
                     VKGAPHNMPLRKLDDVKAARELDLTWKPAS"
BASE COUNT         1038 a          787 c          682 g          942 t
ORIGIN      
        1 atcatattgc aattttcgcc ggaatcggtg atcgtattga aattacccat aaagccagta
       61 accgtaaaac atttgcactg ggtgcactgc gcgcagctca atttttaatg cggcaagaaa
      121 agggtttatt cgacatgcag gatgttctcg ggttgcgaca aggcctataa atcggtagct
      181 gatttttatg gcaacttatg caattggtga tttacagggt tgcttcaaac agctccaaca
      241 cttgcttgag tttatcgcat tctcaccttc gcgcgacagg ctatggtttg taggcgatat
      301 cgttaatcgc ggccccgatt cgctcagttt attacgcttc atcagtcaat tgggtgattc
      361 agctacgatt gtattaggca atcatgattt acatttatta ctggttgcgg aagaaatcac
      421 ctcgcaacgg ggtggcgata ccttacaagc aattctcaat gcgccagatc gtaatcaatt
      481 actttattgg ctccggcacc aaaaaatgct tcatgtcgaa gatgattatg ttctggtcca
      541 tgcaggatta ttaccatcat ggcctgcttc gtttgcccta gaactggccc aagaagtcga
      601 agcaacatta caaagcaacg attacaccga cctattactg catttatatg gcaatcaacc
      661 cgatcaatgg catgatgacc taaaatccta cgatcggtta cgcttaatcg ttaatgcatt
      721 aacgcgacta cgcatctgca cgaaagaagg gaaaatggat ttttcttata aaggacccct
      781 agaaacgatt cccgaaggct atttcccttg gttcgatgtg ccgagtagac aaagccaaaa
      841 atcaaccatt atttttgggc attggtctgc cataaggcta cagctaagac ataatctcat
      901 tgcattggac accggatgct tgtggggcgc tgagctcact gcgattcgct tggaagatcg
      961 gaagattttt cagatccctt gcgtacaacc ggtaaagcga gctggtgtga aatcgcttgt
     1021 tcggtaagtc gtgctaattc tcgtcgattc ttttcctcgc cctgtaatgg gtccagaaaa
     1081 gttaattcaa catggcaact cggttgttgc agtatcaatt gtaatgattg ggtgatcgaa
     1141 acatcgacat acgctgttag cgtagtaggc tgattcatgg catctcgata atgaatcgct
     1201 actggataaa ccaatccctt cgaagccacc gcaaactgca gcaatgatgc atgaaaatga
     1261 agcagatgcg tcccatcgct cgtcgtacct tctggaaaaa tagcaatacg atcacccgct
     1321 attaaaattt cactgagaaa ttgattaact cttaaagtgt cattgcgttt ttcgcgtgcc
     1381 acaaaaattg tacctgcgtt agcagacaat tttccaatta tcggccagtc tagaatttct
     1441 gacttagcaa caaagcggac gggatagatt gcaagaatcg caatgacatc aagccaagaa
     1501 atatggttgg cagccagtat cacgcgtgcc tgatcatcgc taggaagtga tccatgatag
     1561 tgcagtttga tattaagaat acgcagaaag cgttgcgccc atttcttagc catataacgc
     1621 tgcgtacggc gatttacatg gggataaata aacgactgta gtaatcctga tacaaagtga
     1681 atgattaaac agagaaagcg gaaaaattga agaaacttat gagtcttaac gtggctcgtc
     1741 atggaatggg atagaaccaa taaaattgtt attcagccat gaattaaagc ccccacccac
     1801 aataagcttt aataggattc gtacaataac ctaaaaaata gcggttattg tctagaaaat
     1861 acgatttcca taagcttcaa aatagtgctg atcaatagca gaacgatcaa attcatgatt
     1921 ttgaccaccc cgataagcaa ttttgagtgc ccctagtaat gaagccagtt ttcctgttgc
     1981 ataccaatcc aaaccatgag caattccata aagcaaacca gcccgatacg catcaccaca
     2041 acccgttgga tctacaatcg ctttgggctg gacacttgga atctcgatat gatgcccgcc
     2101 cgtgtagatg acggaacctt gagccccttt agtaacaatt aacgctttaa ccagactagc
     2161 taattttccc aaggaatacc ccgttcgctc caataacaat tcagcttcat aatcattaac
     2221 agtcacatag tctgctttgc taatgaaatc aaccaactca tcgccactga acatcgggag
     2281 accttgccct ggatcaaaaa tgaaagggat tccacattct tgaaactcac gtgcatgagc
     2341 aatcattcca tctcgtccat ccggtgcaac gataccaagg ctaacatgct tagcctcttt
     2401 caccgagtta tgatgcgaga aattcattgc gccaggatga aaagcagtaa tctggttatc
     2461 atctaaatca gtggtgataa aagcttgtgc agtaaaagta tcatcaatct ggcgaatatg
     2521 cgttttatca aaacccactt tctcacagcg atacgcgtac ggttgaaaat catcacccac
     2581 cgttgccata atgaggggat cttcacccaa cattttaaga ttataggcaa tattagctgc
     2641 gcacccgcca aattcacgcc gcatttcagg cactaggaat gccacattga gaacatgaat
     2701 tttttccgga agaatatgat gtttaaagtg atcttgaaac accataatcg tatcgtaggc
     2761 aatcgaacca cagatgaggg tacgcataat tttcgtaact caataatcgt tataaagaat
     2821 aaatttaatg ggcactgcta acttcgtgcc cattaaattg acgatcaatt caacactaac
     2881 tcgcaggctt ccaagtcaaa tccaattctc ttgccgcttt aacatcatca agcttgcgga
     2941 gcggcatatt gtggggtgca cctttaacca tatcgggttg ctctttaatt tcaactaaaa
     3001 tttctttcat cgcagcaaca aacgcatcta atgtttcctt tgattcagtc tcagcaggtt
     3061 caatcagcaa gcactctgga acaagaatag ggaaataagt tgtcggtgca tgatagcctt
     3121 tatctaacaa tcgtttagcc agattcattg cagtaacacc cgttctatct ttaatttctt
     3181 taagcgtcac aatgaattca tgactagcgc gtcgcgttgg gtaagcaatt tcaaagccca
     3241 cttcacgtaa ttctgccatt aagtaattcg cattcagcgt tgcaaattcc gaaatccggt
     3301 gcattccatc aaagcctaac aaacgcacat aaatataagc acgcagtaac acacccgcgt
     3361 tacccatatg cgcagacaat cgaccgatcg atttaggtaa atctttttca gtcaaccagc
     3421 gatagtcccc tttttctgtc ttagcaact
//
LOCUS       DAOQLJ010000214         3449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_14485_length_3449_cov_6.013553, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000214 DAOQLJ010000000
VERSION     DAOQLJ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14485_length_3449_cov_6.013553"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PK522_11060"
     CDS             complement(<1..706)
                     /locus_tag="PK522_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNC42312.1"
                     /translation="MIDSKHHSNILWHSQALEEIITSLETSANGLSQVEAALRLQRDG
                     YNRLNPPRQISSLMRFLLQFHNVLIYVLLLGALITGFLGHWVDTGVIVGVVFINAIIG
                     SVQQGKAEKALSAIRHMLSLDAMVLRDGKRKQIPAEELVVGDIVLLQSGDKVPADLRL
                     LQVKNLRIEEAALTGESEAVEKSIATVAEQAVIGDRFNMAYSSTLVVYGQGTGVVVAT
                     SDQTEIGRISRLLEQVD"
     gene            782..1306
                     /locus_tag="PK522_11065"
     CDS             782..1306
                     /locus_tag="PK522_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HNC42313.1"
                     /translation="MNIFRFGLLVMTALLAIISHASTQVPFGNEIKTVKNYNRATTQL
                     ATSGSLNEGSVQELAQHGFKTIIDLRTEAEGTASEKTAVESAGMTYINIPVTGAGVDA
                     EQLATFINAFEQAATPVLLHCASGNRAGAMWTTYRLHKGIAADIAFEEGRTAGMQPSL
                     EEKIKLTCQANKSC"
     gene            complement(1317..2444)
                     /locus_tag="PK522_11070"
     CDS             complement(1317..2444)
                     /locus_tag="PK522_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNC42314.1"
                     /translation="MLERHDTLECLNQTSPLREKLVKTHMVLNNHLPFIKRIAVAIYD
                     PKTSVLKTYLHSSGKEDPLSHYQTAIKDTPSLEALMKQGNPRIIKNKVTVERGRHEHT
                     QRIGREGYAASYTLPMFYNGVFFGFIFFNSSETDAFNETTLEQLDLYGHLISLMVINE
                     INSVQTLVAAISTASHMTHLRDPETGSHLDRMSRYARLIAKSLAEQYQLDDEYIERVF
                     MFSPLHDIGKLGIPDQILLKPGKLTEEEQTIMRTHATKGREMIDNMLANFGFDSIPDV
                     DILRNIAQFHHESINGQGYPAGLSGNSIPLEARIVAVADVFDALTSRRPYKEAWSNQE
                     AFVMLERMSGEKLDTDCVKALIDQQEEIEQIQEQFKEDPLG"
     gene            complement(2630..3373)
                     /locus_tag="PK522_11075"
     CDS             complement(2630..3373)
                     /locus_tag="PK522_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNC42315.1"
                     /translation="MKNNRRNYYRILHVQPDAPLEIIRASYRAMMGPLKMHPDMGGNH
                     EVAALINQAWEVLSDPAKRAAYDRKIESNLPKTARKASSNRFTQTTQEKKHTNAPHQR
                     ADCSFNLSVCLFCTTPLPLEIGPETCCRSCGCPLAPPPMPAKMGKELFGRRRLPRVQR
                     SQSVIVHVTQEGGPLPAKLRNISDSGMSLELEAMVREGEVIRVLAHEFEALTQVLAVR
                     WYSQRWIASMRLVSIKFAKRQGVFISTYA"
BASE COUNT          958 a          772 c          706 g         1013 t
ORIGIN      
        1 tatcaacttg ctccaacaag cggctaatgc gaccgatttc agtctgatca ctggttgcaa
       61 ccacaacccc ggtgccttgg ccataaacga ccaatgtact cgaataagcc atattaaatc
      121 gatccccgat tacagcctgt tctgctactg ttgcaattga tttttcaacc gcttccgatt
      181 caccggtgag tgccgcttct tcaatgcgta aatttttaac ttgaagtaac ctcaaatcgg
      241 caggtacctt atccccagat tggagcaata cgatatctcc aaccaccaat tcttcagcag
      301 gaatttgttt gcgtttgcca tcccgaagta ccattgcatc gagcgatagc atgtgtcgaa
      361 ttgcacttaa tgctttttct gctttacctt gttgtacaga cccgatgatg gcattaatga
      421 aaacaacacc tacgattacc ccagtatcaa cccaatgccc aagaaatcca gtgataaggg
      481 cgccgagtaa cagtacataa atcaatacgt tatgaaattg cagcaaaaac cgcatcaagc
      541 tacttatctg gcgcggcggg ttcagtcgat tataaccatc gcgttgtagt cttaacgctg
      601 cttcaacttg agaaagccca tttgccgatg tctcaaggct agttataatt tcttcaagtg
      661 cttgactgtg ccatagaatg tttgagtggt gcttagaatc gatcacgcta ccctcttaat
      721 taactggtta aattatgcta agttttaaat gttgcggttt aatcttttac tggagttttt
      781 gatgaacatc tttcgatttg gtttattggt aatgacagcc cttctcgcga ttatttccca
      841 tgctagcacg caagttccgt ttggtaacga aattaaaact gttaaaaact ataaccgcgc
      901 tacaacacaa cttgctactt ctggttcgtt gaatgaagga agtgtgcaag aacttgctca
      961 gcacggattc aaaacaatta tcgatttgcg tactgaagct gaaggtacag catccgagaa
     1021 aaccgctgta gaatcagcag gcatgactta tattaatatt ccggttactg gcgcaggagt
     1081 tgacgctgag caattagcta catttattaa cgcctttgag caagcagcta caccggtttt
     1141 attacattgt gcgtcgggta accgagcagg tgcgatgtgg acaacttatc gattgcataa
     1201 aggaattgct gctgatattg cctttgaaga aggtcgcacg gcagggatgc agccgagttt
     1261 agaagagaaa attaaattga cctgtcaggc aaataaatcc tgctaaaagc cacatactag
     1321 cctaatggat cttctttgaa ctgttcttga atttgctcga tttcttcttg ttgatcgatg
     1381 agtgctttaa cgcagtctgt gtcaagtttc tcaccagaca tgcgctctag cattacaaat
     1441 gcttcttggt tactccatgc ttctttatag gggcggcggc tggttaaagc atcgaataca
     1501 tctgcaacgg ccacgattct agcctcaagc ggtattgaat tgccggatag tccggcggga
     1561 tagccctggc cattgatact ttcatgatga aattgtgcaa tatttctcaa aatatcgaca
     1621 tccggaatac tatcaaagcc aaaattagcc aacatattgt cgatcatttc tcgccctttg
     1681 gtggcatgtg tgcgcatgat cgtctgctct tcttccgtca gctttcctgg ttttagtaaa
     1741 atttggtcgg gtatgcccag ttttccgata tcgtgtaacg gtgaaaacat aaatacccgc
     1801 tcgatatatt cgtcgtcgag ttgatattgt tctgcaagcg attttgcaat gagtcgcgca
     1861 tagcgtgaca tgcgatccaa atggctaccg gtttccggat cacgtaaatg agtcatatgc
     1921 gacgcagttg aaatcgcggc aaccaatgtt tgaactgaat tgatttcatt aatcaccatg
     1981 agcgaaatca agtgaccata taaatctaat tgctctaatg tagtctcatt aaatgcatcc
     2041 gtttcgctgg aattaaaaaa aataaaccca aagaataccc cattataaaa cattggtaat
     2101 gtgtaactcg ccgcataccc ttcacgtccg atacgctgag tatgttcatg ccgcccacgt
     2161 tctactgtaa ctttattctt aataatacga ggatttccct gcttcattaa tgcttccagc
     2221 gagggcgtat ctttgatggc ggtttgataa tgcgataaag ggtcttcctt gccactgcta
     2281 tgcaggtaag ttttcaatac ggaagtcttg ggatcataaa tagccacagc aatgcgctta
     2341 atgaatggta aatggttatt tagcaccata tgggttttaa ccaatttttc ccgcagtggg
     2401 ctcgtttgat tcaagcactc taaagtatca tgtcgttcca gcataatgga taaataatca
     2461 ggcgattaat tagaagtaaa agcagcgcgg tcaacatcca cgtgcttacc atgccgacat
     2521 gatagtcgaa tttattttga gtaatcgtac aaaaaacgac ttaaaaaata ggtaattagc
     2581 ttattcaaac tgctaatcta tattgaaatg acaaagcggg ctcattgagc taggcataag
     2641 ttgaaataaa aacaccttgg cgttttgcaa acttaattga aactagtcgc atgctggcaa
     2701 tccaacgctg actataccag cgcacagcca atacttgtgt caatgcttca aattcatgtg
     2761 ccaggacacg aatcacttcc ccttcacgta ccatggcttc cagttcaaga ctcatcccac
     2821 tatctgaaat attacgtaat ttagccggta acggcccccc ttcttgagtc acatgtacga
     2881 tcaccgattg agatcgttgc acacggggta atcgccgccg cccaaacaat tccttaccca
     2941 ttttagcagg catcggtggc ggcgctaacg gacatccaca agaacggcaa cacgtttcag
     3001 gtccaatttc tagtggcaaa ggtgtcgtac aaaacaagca gactgataga ttaaatgaac
     3061 aatcagcccg ctgatggggt gcattggtat gttttttttc ttgtgttgtt tgcgtgaaac
     3121 gattggaaga agcttttctt gctgtcttag gtaaatttga ctcgattttc cgatcataag
     3181 ccgcacgttt ggctggatcg ctcagcactt cccaagcttg attgatgagg gcagcaactt
     3241 catgattccc ccccatatca ggatgcattt tcagcggtcc catcatcgcg cgatagcttg
     3301 cacgaatgat ttccagcggt gcatcaggtt gaacatgcaa aatacggtag taatttcgac
     3361 gattattttt catttttttg atatcggaca ccatcgactt ggcttaaata aatcctcctg
     3421 gttactttag ttacttcctt atacgaaat
//
LOCUS       DAOQLJ010000215         3435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_14592_length_3435_cov_5.157396, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000215 DAOQLJ010000000
VERSION     DAOQLJ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14592_length_3435_cov_5.157396"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..894)
                     /gene="cysT"
                     /locus_tag="PK522_11080"
     CDS             complement(61..894)
                     /gene="cysT"
                     /locus_tag="PK522_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNC42316.1"
                     /translation="MSQFKQHSVLPGFNLAIGFTLLYLSLVVLIPLSAAFIRATELTW
                     SEFWTVVTTPRVMASYRLTFGASFIAALINAIFGLLVAWILVRYQFTGKKIIDALIDL
                     PFALPTAVAGITLTALYAGNGWFGQFLEPLGIKVAYTPIGVFVALTFIGLPFVVRTVQ
                     PVLEDIETELEEAAAMLGASRLQTFVRIIFPTILPALMTGFALAFARAIGEYGSVIFI
                     AGNMPMVSEITPLLIITKLEQYDYAGATATAVVMLVTSFVLLLIINLLQWWGRRRNAL
                     I"
     gene            complement(947..1945)
                     /locus_tag="PK522_11085"
     CDS             complement(947..1945)
                     /locus_tag="PK522_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC42317.1"
                     /translation="MKLQTWFTVLFIITSLSIIGPARADKTLLNVSYDPTREFFQEFN
                     AAFSKHWLANSGEKITIQQSHGGSGKQARSVIDGLAADVVTLALAGDIDAIAEHGKLL
                     PENWQTRLPRNSSPYTSTIVFLVRKGNPKGIKDWHDLANGGVAVITPNPKTSGGARWN
                     YLAAWEFGKHYFGETRVQEFVSDIYKNVPVLDSGARGSTTTFVERGIGDVLIAWENEA
                     YLALKEYGNDKFEIIVPSVSVLAEPPVAIIDKVVDKKGTRVIAEAYLNYLYSEEAQHI
                     AAKHYFRPSDETIAAQYAQQFTKVKLFKISDTFGSWKNIHQIHFSDGGIFDQIYLK"
     gene            complement(1992..>3435)
                     /locus_tag="PK522_11090"
     CDS             complement(1992..>3435)
                     /locus_tag="PK522_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNC42318.1"
                     /translation="STSYHRRSDSYATNPESDPPRYVRRLSDIGVESFKDVTWLEVGL
                     EHRTRYEWRNNDIRRIEGGEDNPIFLRNRAWIGIKDILDPFRFAVEFQDSRVYNNKHA
                     ITDQERNEYDLINAYGELHFKKGLGVDDRGQDRPIRFRVGRFAYESTDRRFIARNEWR
                     NTTNSFEGFRLTLGREVNDWELDFFGMQPVMRLQTKFDKANENLWFFGAVGTWRKWSD
                     YITLQPYYMGQKQTADPNGFTATNRLDREIHMPGFRAYGRLGIPGQTYILDFDASYNQ
                     QLGFSGPLKQDAHGYTIEIGRTFDHPWKPRISTFYGYASGDRDPNDGEDNRFDRFFGF
                     ARPWSADHYVIYENLKAPKIRFEFQPTPILGFEMGYGAYWLASGTDRMFDILDGNISN
                     TIKDPGFNRDRTGKSGDFGGHALEGRIRFQPTPRIQTILGYTHFTAGEFVKNRIAASP
                     SCADGHAHPCVDRRSGNTDFLYFEVLISLL"
BASE COUNT          965 a          737 c          812 g          921 t
ORIGIN      
        1 agtaattcga gtgcgactag gggaatgaga ggaactaaca gttatagtag ccatatccat
       61 ttatatcagt gcattgcgac ggcgtcccca ccattgcagt agattaataa taagaaggag
      121 cacaaaagaa gtaacgagca tcacgacagc agttgcggta gcacccgcgt aatcatattg
      181 ctcaagttta gtaatgatta gtagcggggt gatttccgaa accatgggca tgtttccagc
      241 gatgaaaatg actgaaccgt attcaccgat ggcgcgagca aaagctaagg caaatccggt
      301 catcaatgcc ggtaggattg ttgggaaaat aatccgaacg aacgtttgta gccgacttgc
      361 gcctaacatt gctgcagctt cctcaagctc tgtctcgata tcttccaata cgggttgtac
      421 ggtgcgaaca acaaagggga gtccaatgaa ggtaagcgca acgaaaactc cgataggggt
      481 gtaggctact ttgatgccaa gtggctctag aaattgacca aaccaaccat tgcctgcata
      541 aagcgctgtt agcgttatgc ctgcaacggc ggttggtagc gcaaacggaa gatcgatcag
      601 ggcatcaatg atttttttac cggtgaactg atagcgcacg agaatccatg caacgagcaa
      661 accgaatatg gcattgatta gcgcagcgat aaaggaagcc ccaaaggtca gtcgatatga
      721 agccatcacg cgaggggtgg ttacaaccgt ccagaattca gaccaggtta gttctgtagc
      781 acgaataaat gcagcagaaa gaggaatgag tacaactaag ctcagataaa gcagagtgaa
      841 gccgattgcc agattgaatc caggtaatac gctatgttgt ttaaattggc tcatttttga
      901 actatccgaa gattattgga taaatctaga atcatctggc aataggctat ttcagataaa
      961 tttgatcgaa gatgccgcca tcactgaaat gaatttgatg aatatttttc cagctaccga
     1021 aggtatcgct tattttgaat aatttcactt tagtgaattg ctgcgcgtat tgagctgcga
     1081 tagtttcgtc gctgggacga aaataatgct tagctgcgat gtgttgggct tcttccgagt
     1141 aaagataatt gagataggct tcagcgatta cccgagtacc ttttttgtcg accactttgt
     1201 cgatgattgc aactggaggc tctgctaaaa cactgacgga aggaacgata atctcaaatt
     1261 tatcattgcc atactccttg agtgccagat aggcttcatt ttcccacgcg atgaggacat
     1321 caccaatacc ccgttctaca aatgtagtgg ttgaaccgcg tgcacctgaa tcgagtactg
     1381 ggacattttt ataaatatcg gatacaaatt cttgtacgcg cgtttcaccg aagtaatgtt
     1441 taccaaactc ccaagcagct aagtaattcc aacgtgcgcc tccggatgtt ttgggattgg
     1501 gtgtaatgac tgcaacacca ccattcgcca aatcgtgcca atctttgatt cctttggggt
     1561 tacctttacg cactaagaaa acgatggtgg atgtgtaggg tgagctgttt ctaggtagac
     1621 gagtctgcca attttcgggt aatagttttc catgttcggc aatggcatcg atatcgccgg
     1681 ctaacgcgag tgttacaacg tcagccgcta aaccatcgat tactgaacgt gcttgtttac
     1741 cggagccgcc atgcgattgt tgaatagtta ttttttcacc gctgtttgct aaccaatgtt
     1801 tactaaacgc ggcattaaat tcttgaaaga actctctggt gggatcataa gaaacattca
     1861 gtagggtttt atcagcacga gcgggcccaa tgatggataa gcttgtaatg atgaacaata
     1921 cggtgaacca agtttgtaat ttcatagttt ttcctttcga ttcaaattga tgattaaaat
     1981 tttgttgaga attaaagtag gctaataagt acttcaaaat ataagaaatc ggtattgcct
     2041 gagcgacggt cgacgcaagg gtgtgcatga ccatcggcgc aagatgggct agctgcaatc
     2101 cgatttttta cgaattcacc tgctgtgaaa tgggtataac ccaatatcgt ttgaattctt
     2161 ggtgtaggct gaaagcggat acggccttca agcgcatgtc cgccaaaatc cccacttttt
     2221 ccggtacgat cccgattaaa tcctggatct ttgatcgtat tgctgatatt gccatcgaga
     2281 atatcgaaca tacgatcggt gccgcttgct aaccagtaag caccataccc catttcaaac
     2341 ccgagtattg gggtaggttg aaattcaaag cggattttag gtgctttcaa gttttcgtag
     2401 atgacataat gatcagccga ccaagggcgc gcaaatccaa agaagcggtc aaagcggtta
     2461 tcttcaccat cattagggtc acgatcgccc gatgcatagc catagaaggt gctgatccgt
     2521 ggtttccaag gatgatcgaa ggtacggcca atttcgatgg tataaccatg cgcatcttgt
     2581 tttaaagggc cactgaaacc gagttgctgg ttgtagcttg catcaaaatc taagatatag
     2641 gtttgtcctg gaatccctag tcggccataa gcgcggaatc ctggcatatg gatttcgcgg
     2701 tctaagcgat tggttgcagt aaagccatta ggatccgctg tttgcttttg acccatatag
     2761 tagggttgca acgtgatgta atcagaccat ttgcgccagg taccgactgc accgaagaac
     2821 catagatttt cgttggcttt gtcaaattta gtctgcaagc gcataactgg ttgcatacca
     2881 aagaagtcaa gctcccaatc attaacctcc cgaccaaggg ttaggcgaaa gccttcaaat
     2941 gagttcgtgg tattacgcca ttcattgcgt gcgataaagc gccggtctgt cgattcatag
     3001 gcaaagcgac cgactcggaa gcgaatggga cggtcttggc cgcgatcatc aacaccaagc
     3061 ccctttttga aatgcaattc accatacgca ttgatgagat cgtactcatt tctttcctgg
     3121 tcggtaatcg cgtgtttatt attgtagacg cgtgaatctt gaaattcgac tgcaaaccgg
     3181 aacggatcta atatatcttt gataccaatc caagcgcggt tacgtagaaa aattggatta
     3241 tcttccccgc cttcaatgcg ccgaatatca ttattacgcc actcgtagcg ggtacgatgt
     3301 tccaatccaa cttcaagcca tgtgacatct ttaaaggatt caacaccgat atcgcttaag
     3361 cgccgaacgt agcgcggtgg atcagattcc gggttggtag catagctgtc cgaacgacgg
     3421 tgataacttg tggat
//
LOCUS       DAOQLJ010000216         3433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_14604_length_3433_cov_6.476317, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000216 DAOQLJ010000000
VERSION     DAOQLJ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14604_length_3433_cov_6.476317"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..755
                     /locus_tag="PK522_11095"
     CDS             108..755
                     /locus_tag="PK522_11095"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HNC42319.1"
                     /translation="MRILSLPNFWLLIALVSLIIIYPIAYPYSSGRAFLALFDWIILI
                     LALRASKKTWYKSWLGYAFAIPAMSFYVIAALFSSSVVIAASLVTQALFHGFIVISLL
                     RYMLHDNVMTLDELFAAAALYVLIGFVFGYCYVLIEHIHPGSFYINEANNPDQIVSWW
                     ELLYFSFTCLTSVGFGEITPISDSARSIVIIEQTSGVLYLAILISRLMSMRMKRE"
     gene            complement(785..1378)
                     /gene="scpB"
                     /locus_tag="PK522_11100"
     CDS             complement(785..1378)
                     /gene="scpB"
                     /locus_tag="PK522_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNC42320.1"
                     /translation="MSDQFSLTLPVSQSTSFSNQADQIRILEAALLTSQEPLPIAELK
                     KLFDGTINSKQLLTMLDELSNQWQEKGIELVEVASGWRFQSRPEMQVYLEKLNPQKPP
                     RYSRAVLETLAIIAYRQPVTRGDIEEIRGVTVSSAILKTLETRGWIEVVGQRNVPGRP
                     QLYATTSNFLNDLNLQSLDQLPPLDELVNQTEHATDE"
     gene            complement(1362..2153)
                     /locus_tag="PK522_11105"
     CDS             complement(1362..2153)
                     /locus_tag="PK522_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HNC42321.1"
                     /translation="MDELNSLVEAHPIAKICGQPLHEVPHDLYIPPEALEIFLDAFEG
                     PLDLLLYLIRKHNLDILDIPMAELTQQYMAYVEAMRADQLELAAEYLLMTALLIEIKS
                     RMLLPKPIHTPEEEADPRAELVRRLLEYEQMKKAALRLDELPQLERDFMQVQVLINQT
                     VIQQLVQVNLDDLQQAWLTLLQRAKLSTRHHVQFEAISVRSCMSEILRKLKQAGTVSF
                     YDLFSPSPAIGEVIANFLAILELAKELLIEIEQTEVFGTIYVRSI"
     gene            complement(2203..3405)
                     /locus_tag="PK522_11110"
     CDS             complement(2203..3405)
                     /locus_tag="PK522_11110"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNC42322.1"
                     /translation="MFADRVLSGMRPTGHLHLGHYHGVLKNWVTLQQQYECLFFVADW
                     HALTTHYDSPEMIEQHTWEMIIDWLAAGVDPAQATLFIQSKVPAHAELYLLLSMITPL
                     GWLERVPTYKDQQEKLTEKDLSTYGFLGYPLLQSADILIYRANRVPVGEDQAPHLEFT
                     REISRRFNHIFGKEPGFEDKAEQAIKKLGSKRSKLYRELRTRYQEQGDESALEEGRSL
                     LDEQQNLSLGDRERLFGYLEGGGRVILAEPETLLTQASRMPGLDGQKMSKSYDNTISL
                     REDADTIRKKIRTMPTDPARIRRTDPGDPAKCPVWSFHAVYSNDEVRNWVQQGCKNAS
                     IGCLECKQPVIDAILAEQIPICERAKMYEEDPALVRNIVADGCEKANKLAEETMRDVR
                     QVMGLSYS"
BASE COUNT          894 a          701 c          743 g         1095 t
ORIGIN      
        1 gaaaagggtg agaaataatc agttaatttt tctcgatcaa gcttaaaaat taaggttatt
       61 tgtgtataaa actccttaac gctttaacca ttaaaataag atagcgcatg cgcattttgt
      121 ctctccccaa tttttggtta ctgatcgcgc tggtctcgct cattatcatt tatcctattg
      181 cctatcccta ttctagtggt agagcctttc tcgcgttatt cgattggatc atattgatcc
      241 ttgcattacg tgcttcgaaa aaaacctggt ataaatcgtg gctgggatac gcgtttgcga
      301 taccggccat gtcattttac gtcatcgctg cattatttag ctcatctgtt gttatcgcag
      361 caagcttggt tacgcaggca ttgttccatg gctttatcgt gattagctta ttgcgctata
      421 tgttgcacga taatgtgatg acacttgatg agctttttgc tgcggctgct ttgtatgtat
      481 taataggatt tgtatttggt tactgttatg ttttgattga gcatattcat cccggtagtt
      541 tttatattaa cgaagcgaat aacccagatc agatcgtatc ttggtgggag ctgttgtatt
      601 tcagttttac ctgtttgacg agtgttggtt ttggtgaaat tacgccaatc tcagattcag
      661 cgcgttcgat tgtgattatc gagcagacat cgggtgtatt gtatttagcg attttaattt
      721 ctcgtttgat gtcgatgcgc atgaagcggg agtaggtgtg ctggttttcg atcgttttcg
      781 ctactcattc atctgtcgca tgttcagttt gattgaccag ctcatctaaa ggcggtagtt
      841 gatccagtga ttggaggttt aagtcattca gaaaattaga ggtggttgca tagagttgcg
      901 gtcttccagg aacattacgt tgcccgacaa cttcgatcca accccgtgtt tccagtgttt
      961 tgagtatcgc actggaaacg gtgacacctc taatttcttc gatgtcaccg cgggttaccg
     1021 gttgacgata agcgatgatt gcaagggttt ccaatacagc gcgtgaataa cgtggtggtt
     1081 tttgtggatt aagtttttcc agataaactt gcatttcggg tcgactttgg aagcgccatc
     1141 cgcttgcaac ctctaccagt tcaatccctt tttcttgcca ctgattgctt aattcgtcca
     1201 gcatcgtgag taattgttta ctattgatgg tgccatcgaa tagttttttt aactcagcaa
     1261 tcggcagtgg ttcctgagaa gtcaataacg ctgcttccag aattcggatt tgatcggctt
     1321 gattggaaaa cgaagtagat tgagaaacag gtagagtcag gctaaattga tcggacatag
     1381 atggttccaa aaacctcagt ttgttcgatt tcaatgagta attcttttgc taattcaagg
     1441 atggcaagaa agttcgcaat tacttcacct atggctgggc taggtgaaaa taaatcgtaa
     1501 aaagaaaccg ttccagcttg ctttagtttc cgcaaaattt cactcatgca agagcgcacg
     1561 gatattgcct caaactgaac gtgatgccgc gtactcagtt ttgctcgttg aagcagagtt
     1621 aaccatgcct gctgtaaatc atcaaggttg acttggacaa gttgctgaat aactgtttga
     1681 ttaatcaaaa cttgcacttg catgaaatcg cgctcgagtt gcggtaattc atccaagcga
     1741 agggctgctt ttttcatttg ctcatattct agcaaacgac gtacaagctc ggcacgggga
     1801 tctgcttctt cttcaggggt atgaatcggt ttaggaagca acatgcgtga tttaatctcg
     1861 attagcaatg cagtcattag caaatattct gccgctagct ctaattgatc ggcacgcatc
     1921 gcctcaacat aggccatata ttgctgggtt aactcagcca tggggatatc taaaatatcc
     1981 aagttatgtt tgcgaattaa gtaaagcaat aaatcaagcg gaccttcaaa cgcatcaaga
     2041 aaaatttcta atgcttcagg tgggatatat aagtcatgcg gtacttcatg taacggttga
     2101 ccgcatattt ttgcaatggg gtgagcctca acgagtgagt ttagctcatc cataccattg
     2161 gctcctgtgc taaatcaatt aattaatggt taacgaaaat gatcaagaat agcttaatcc
     2221 catgacctgc ctaacatcac gcatagtttc ttctgctagt ttattggctt tctcacagcc
     2281 atccgcaacg atatttctca ctaaggctgg atcttcttca tacattttag cgcgctcaca
     2341 aattgggatt tgttctgcta agatggcgtc aataacgggt tgcttacatt ctaggcaacc
     2401 aatacttgca tttttacacc cttgttgaac ccaattcctg acttcatcat ttgaataaac
     2461 cgcgtgaaat gaccaaactg gacatttcgc ggggtcacca gggtcagtac gacggatgcg
     2521 agcgggatcg gttggcatgg tgcgaatttt tttgcggata gtatcagcat cttcacgtaa
     2581 actgatcgta ttatcatatg atttagacat tttttgcccg tctaaccccg gcattcgcga
     2641 tgcttgggtc aataatgttt caggttcggc caaaatgact cttcctcccc cttctaaata
     2701 accaaacaat ctttctcgat cacctaagct taaattttgt tgttcatcga gtagtgaccg
     2761 cccttcttct aatgcacttt catcgccttg ttcttgatag cgcgtgcgta attcacggta
     2821 aagcttggag cgcttcgatc ctagtttttt gatggcttgt tcggctttat cttcaaaccc
     2881 gggttcttta ccaaaaatat gattgaagcg gcgggaaatt tcgcgcgtaa attcgaggtg
     2941 aggtgcctga tcttcaccaa caggaacgcg attggcgcga taaataagaa tatctgcact
     3001 ttgcagtaaa ggatagccta aaaatccata cgtacttaaa tctttttcag tgagtttttc
     3061 ttgttgatct ttataggtag gaacgcgttc aagccaacct aatggcgtga tcatggaaag
     3121 cagtaaatag agttctgcgt gggcaggtac tttggattga atgaacaatg tggcttgcgc
     3181 tgggtcaacg cctgcagcta accaatcgat gatcatttcc caagtatgtt gttcgatcat
     3241 ttcgggtgaa tcataatgcg tggttaatgc atgccaatct gccacaaaaa acaaacattc
     3301 atattgttgt tgtagcgtta cccaattctt tagaacacca tggtaatgtc ccaaatgcaa
     3361 atgacccgta gggcgcatcc ctgataaaac acgatcagca aacatgatta attttccaaa
     3421 ttaattcgtg ata
//
LOCUS       DAOQLJ010000217         3433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_14605_length_3433_cov_4.707519, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000217 DAOQLJ010000000
VERSION     DAOQLJ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14605_length_3433_cov_4.707519"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..624
                     /locus_tag="PK522_11115"
     CDS             25..624
                     /locus_tag="PK522_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42323.1"
                     /translation="MLLLESMTNTDWFTLTLVLITAFYAYATFKILRANEAMVTTMRD
                     QQNAAMRPYIIISANVRMGTQLFYLSIKNVGMTAAIDLSLSMDKNFYQFGEKREERNI
                     ANRVAFSQTINSLPPDGQFLFLLGNGPTLYGESNSDELSPLLFTVTANYRSITDSYTE
                     TSVVDLRPYINTDAPYDPIVEELRNLRQEVTKLAKLLEK"
     gene            complement(672..869)
                     /locus_tag="PK522_11120"
     CDS             complement(672..869)
                     /locus_tag="PK522_11120"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC42324.1"
                     /translation="MLSPVLLELEQVKQHQGEILKQCATLFDNNKLTVSVTHTFSLSE
                     AATAQHFLEQNHPIGKLVLLA"
     gene            complement(1152..1427)
                     /locus_tag="PK522_11125"
     CDS             complement(1152..1427)
                     /locus_tag="PK522_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNC42325.1"
                     /translation="MKNNEPLPTAVALAYREGQTAPKVVAKGRGLIAEEIIHRAKEAG
                     IYVHESRDLVALLMQVDLDDRIPPQLYVAVAELLAWLYKLEHSKYSP"
     gene            complement(1417..2436)
                     /locus_tag="PK522_11130"
     CDS             complement(1417..2436)
                     /locus_tag="PK522_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNC42326.1"
                     /translation="MSPITAKPDLITPLTQIKSITPVSTDLDSQKNSSEFLPGQKYQA
                     LVDARLSNGNARVLIANQWFQMQLPENFQSGAKIELLVTSRLPELQFQIINGTQEQAS
                     DQPATISSTGKLISALLQDAHKQSTPTSQNTQISPSISSESSINSTELPSLLQKAISQ
                     SGLFYESHQAQWINGEKTLQSLHQEPQNKMNSTVMAAMDESVVRAVAASSNTMDVVVN
                     NQINTLVTQQLSTLETGQVVWRGEIWHNQPMEWEIYEQPEDHKQNEHEQTVQWHTKLH
                     LTMPNLGEITAKIAFGPHGLTIKLDAIDRNTIDLLKNNQQPLASQIQAAGLNVQSVEI
                     HQYEK"
     gene            complement(2534..2779)
                     /locus_tag="PK522_11135"
     CDS             complement(2534..2779)
                     /locus_tag="PK522_11135"
                     /inference="COORDINATES: protein motif:HMM:NF017237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HNC42327.1"
                     /translation="MVAAKNGEWDLLISLEKECKHLTDLLKEKKPEPNLSDELLQEKI
                     EIIHQILEDDDQIRAITEPWMIRLQEILCANGYNRSL"
     gene            complement(2943..3140)
                     /locus_tag="PK522_11140"
     CDS             complement(2943..3140)
                     /locus_tag="PK522_11140"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC42328.1"
                     /translation="MLSPVLLELEQAKQHQGEILKQCAALFDNNKLTVSIVRTFKLSE
                     AATAQHFLEHNHPAGKVILVV"
     gene            complement(3058..3267)
                     /locus_tag="PK522_11145"
     CDS             complement(3058..3267)
                     /locus_tag="PK522_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42329.1"
                     /translation="MLALKPRLLSFEQAAAAPLVFITAWEALHDRARIAPDQIVLIHA
                     VTGFVGARTSQATSRRNIKAMRRIV"
BASE COUNT          911 a          702 c          738 g         1082 t
ORIGIN      
        1 aagaaataat caaatctagt tgctatgctt ttacttgaat ccatgacgaa tacagattgg
       61 tttacgttaa cgctcgtact tattacagcg ttttatgcgt acgctacttt caaaattctt
      121 cgcgctaatg aagcaatggt tacaactatg cgagaccagc agaatgccgc tatgcgacca
      181 tatatcatca tatctgcaaa tgtaagaatg ggaactcaac tcttttatct atcgattaag
      241 aatgttggaa tgactgcggc tattgactta agcctctcta tggataagaa cttctaccag
      301 tttggagaaa aacgtgaaga gcgcaatatt gcgaatagag tagctttctc acaaaccatc
      361 aatagcttac cacctgatgg tcaatttcta tttttactag gaaatgggcc aaccttatat
      421 ggtgaatcta atagtgatga gctttctccg ctattattta cagttactgc aaactataga
      481 tctatcactg attcatacac agagacttcg gttgtggatc ttcgtccata tattaatacc
      541 gacgccccct atgatccaat tgtcgaagag ttgagaaact tacgtcagga agttactaag
      601 ctcgccaagc ttctagaaaa atagttttaa tgtgaatggg atctggatat ccttcagagg
      661 ctcgattatt gttatgccaa aagtaccagc tttccaatcg gatgattctg ctccagaaaa
      721 tgttgtgctg ttgcagcctc tgaaagtgag aaagtatgtg taaccgatac agtgagcttg
      781 ttgttatcaa acaatgtggc acattgcttt aatatttcgc cctgatgttg cttgacttgc
      841 tctaactcca acaaaaccgg tgacagcatg aattaaaacg atttgatctt gcgtgattct
      901 tgcccaatca tgcagtgcct cccacgcggt gataaacact aatggtgccg cagctgcttg
      961 ttcaaaggat aataagcgcg gttttaatgc cagcaagttg gcatcgacta ctacaaactc
     1021 ggcgtaagtg ccttgtcggc cattaaaacc gggttgagaa aaataaactt catttccagg
     1081 tttgaggtcg ctaacctttg taccaagtgc ttctacaata cctgcaccat cgcagtaata
     1141 tcaccactga tttaaggtga atacttgcta tgttccagtt tatatagcca ggcaagaagc
     1201 tcggcgacag caacataaag ctgcggagga atacggtcat caagatcaac ctgcatcaat
     1261 aatgcaacca agtctctcga ttcatggaca tagattccgg cttctttggc acgatggata
     1321 atttcttcag cgatcaaacc tcttcccttg gcgaccactt tgggcgcagt ttgaccttcc
     1381 cgataagcga gcgctacagc ggtgggcaga ggctcattat ttttcatact gatggatttc
     1441 aacagactga acattcaagc cagctgcttg tatttgcgag gcgagaggct gttgattatt
     1501 tttcagcaga tctattgtgt tcctatcgat ggcatccagt ttgatggtta atccatgggg
     1561 tccgaatgca attttggctg taatctcacc taggtttggc atcgtgagat gtaatttcgt
     1621 gtgccattga acagtctgct cgtgttcatt ttgcttgtga tcctcaggtt gctcataaat
     1681 ctcccactcc atgggttggt tatgccatat ctcaccgcgc caaacaacct gtccggtttc
     1741 cagtgtggat agttgctgcg tcaccaacgt gttgatctgg ttattcacca caacatccat
     1801 agtattggaa ctagccgcta ctgctctaac tacggattca tccattgctg ccattaccgt
     1861 tgaattcatt ttgttctggg gttcttggtg aagactttgc agtgtcttct ctccattaat
     1921 ccactgtgct tgatgagatt catagaataa tccactttga gaaattgctt tctgcaataa
     1981 acttggtagc tcagtgctgt ttatggaaga ctctgatgaa atggaaggag atatttgagt
     2041 attttgtgaa gtaggcgttg attgcttgtg tgcatcttgt agtagagcgc tgatcaattt
     2101 cccagtagag ctgatcgttg cgggttgatc gcttgcttgc tcttgagttc cattaataat
     2161 ttgaaactgg agttcgggta gtcgtgatgt gactaatagt tctatttttg cacccgattg
     2221 gaaattttcg ggtaattgca tctggaacca ctgattggca atcaggacgc gtgcattgcc
     2281 atttgataat cgtgcatcaa cgagggcctg gtatttttgc ccgggtagga attcagagct
     2341 gtttttttgt gaatcgaggt cggttgaaac aggtgtaatt gatttgattt gagttaaggg
     2401 ggtaatcaga tcaggtttgg cagtaatcgg gctcatgatg attttccccc ttcaactcac
     2461 atcttttttc gttacaaaga tgcatctatt cgggttgaaa cgatcagatg ttctcgaatg
     2521 attgataggt ttgttagaga cttctattgt atccattggc acacaggatt tcttgcagtc
     2581 ttatcatcca tggttcagtg atggctctga tctgatcgtc atcttccaag atctgatgga
     2641 taatttcgat tttctcttgt aataactcat cgcttaagtt tggttctggt tttttctctt
     2701 ttagtaaatc ggttaaatgt ttgcattctt tctctaatga tataagcaaa tcccattcac
     2761 catttttagc agcaaccagt atttttcccg ttgtttctaa aatagctgta cggcaaggaa
     2821 tatcacttat tcatgaagat gagtgcggtt ggagtgacac aaactcaggg tgaattaaga
     2881 attaataaaa atcctatact gagcagaatt gtcatgtcag ttcagcgtat ctacgattat
     2941 ttctaaacaa ctagaatcac tttcccagct ggatgattgt gctctaggaa atgctgcgct
     3001 gttgcagctt ctgagagttt aaaggtacgt acaatcgata cagtaagctt attgttatca
     3061 aacaatgcgg cgcattgctt taatatttct ccttgatgtt gcttggcttg ttctagctcc
     3121 aacaaaaccg gtgacagcat gaatcaaaac gatttgatcg ggtgcaatgc gtgctcggtc
     3181 gtgcagggcc tcccaagcgg taataaaaac cagtggtgcc gctgctgctt gttcaaagga
     3241 taataagcgc ggttttaatg ccagcaagtt ggcatcgact actacaaact cggcgtaagt
     3301 gccttgtcgg ccattaaaac cgggttgaga aaaataaact tcatttccag gtttgaggtc
     3361 gctaaccttt gtaccaagtg cttctacaat acctgcacca tcgcagtaat atcaccactg
     3421 atttaaggtg aat
//
LOCUS       DAOQLJ010000218         3425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_14669_length_3425_cov_5.312760, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000218 DAOQLJ010000000
VERSION     DAOQLJ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14669_length_3425_cov_5.312760"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1691
                     /locus_tag="PK522_11150"
     CDS             <1..1691
                     /locus_tag="PK522_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC42330.1"
                     /translation="ILLHGGAPSLPKAYCEAVHGLRNGPNVGSCGTSTFTGKRVLVEN
                     IETDPKWADIKHFALPHGMRCCWSEPIKNSSGKVLGAFGMYYNHPALPNEQELKDLIS
                     AARLAGIVMERDQAQKRIRALAYTDELTGLASRASFIQHMEEIMKTSDRYNRRFGLLY
                     IDLDNFKWINDSLGHDAGDALLKTIATRLRNTCRDTDFIARLGGDEFCVLVEEIEADY
                     AANVAKRCLAAIAEPIVIYSRNLTPGCSIGIAYYPDDGNNPAALLKAADTSLYTAKSN
                     GKNQYAFYCTELTRKAHYRFQIEQNLKEAIATQQLTLVYQPQVNVITGEIFGVEALSR
                     WHHPQIGSIPPSDFIATAERIGMIKSLTEWVLRTACQQLIVWQTEGFHRLRMAVNISP
                     SLFLETEFVSLVQRIVQETGIKPSQLKLEVTEGVVQTDPRNLAIFQDLKALGVLLAID
                     DFGSGYSSFASLNHLKTDYLKIDKYFIDKMITDQKAFTLVNTMIEMGHKLGYGIIAEG
                     VETLEQLSILRSLNCEIVQGYLFSQPVSAAQLSVLLQSDKHKMILDQCRVNKIA"
     gene            1789..3375
                     /locus_tag="PK522_11155"
     CDS             1789..3375
                     /locus_tag="PK522_11155"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNC42331.1"
                     /translation="MSKIFINYRRSDSAGYAGRLYDRLVKHFGADHLFMDIDQIEPGE
                     DFVEVLQKKLQAVQVAVVLIGKSWLDSKDETGQRRLDNPDDWVRIEIATLLERKIRII
                     PVLIGGAATPKSSQLPELLISLVRRQAFEISDTRFHSDVDKLIQALEKIIQLQLTQLP
                     PKPNLITPDSSIPDDVRHQKLTDAQHSPAQSRSESASVNKKPRSTISRFVMITIGCIA
                     LVFTVLSIVKLPITAQHDSSRDRISSPAPNSVVSESIQLNSEPVASASTEESVPAAGQ
                     QDSNRDGISTSPPERVATVATEQHAEPAVSTRSEESELASMYDFEPQMVRISPGSFIM
                     GSPESEAGRSADEGPQHPVKITYPFEIGRYEVTFAQYDAFAKDTQHQLPDDRGWGRGD
                     RPVINVTWHDAQAYVKWLSDKTGKKYRLPTEAEWEYVARAGTATAYWWGDRIGKANAA
                     CDGCGSQWDGKKTTLVGSFKPNAFGVYDTAGNVWEWTQDCWYGDYTRAPTDGSAWLEQ
                     NGGDCSRRVVRGGSWSSYPQ"
BASE COUNT          968 a          724 c          827 g          906 t
ORIGIN      
        1 atattttact gcatggcggt gcacctagtt tgccaaaagc ctattgcgaa gcagttcatg
       61 gcttgagaaa tgggcccaat gtcggttcct gcggaacttc gacatttacc ggaaaacgtg
      121 tgttagtcga aaatattgaa acagacccta aatgggcaga cattaagcat tttgcgttac
      181 cccatgggat gcgttgttgc tggtctgaac ccatcaagaa ttccagtggt aaagtattgg
      241 gtgcatttgg gatgtactat aaccatcccg cgttacccaa tgaacaggaa ttaaaggatt
      301 taatttctgc agcacgtttg gccggtattg ttatggaacg agatcaagcg caaaagcgta
      361 tccgtgcact tgcttacact gatgaattaa ctggtttagc aagtcgtgct tccttcattc
      421 aacacatgga agaaatcatg aagacatcag accgctataa ccggcggttt ggtttgttat
      481 atatcgatct tgataatttc aagtggatca acgatagttt agggcatgat gccggagatg
      541 ctttacttaa aactattgct acccggctga gaaatacgtg tcgggatacc gatttcatcg
      601 cgagattagg aggagatgaa ttttgtgtgt tggtcgagga aattgaagca gattatgctg
      661 caaatgtagc aaaacgctgc cttgcagcga tcgccgaacc catcgtaatt tattcccgga
      721 atctcactcc aggttgtagt atcggtattg cgtattaccc agatgatgga aataatcctg
      781 ctgcattgct aaaagcagcg gatacctcgc tctatactgc aaaaagcaat ggtaagaatc
      841 agtacgcatt ttattgcacg gaattgacgc gcaaagcgca ttaccgtttt caaatcgaac
      901 aaaatctcaa agaagcaata gcaacgcagc aactaacatt ggtatatcag ccgcaagtga
      961 atgtaattac cggtgagatt ttcggggtgg aagctttgtc gcgctggcat catcctcaaa
     1021 taggctcgat tccaccgagt gattttatcg caacggcgga acgcattggc atgatcaaat
     1081 cgttaaccga atgggtatta agaaccgcat gccagcagct catcgtttgg caaactgaag
     1141 gattccaccg actacgtatg gctgttaaca tttcccccag cctttttctt gaaactgaat
     1201 ttgtctcgtt ggtgcaacgc attgtccagg agactggaat caaaccaagc caattgaaat
     1261 tagaagttac tgaaggggta gtacaaactg acccacgtaa tcttgcaatt tttcaagatc
     1321 tgaaagcgct gggtgtatta cttgcgatcg atgattttgg gagtggttat tcatcttttg
     1381 cttcattgaa tcatcttaag acggattatc tgaaaatcga caaatatttt atcgataaga
     1441 tgattaccga tcaaaaagcg ttcacgctag tcaacacgat gatagaaatg ggacataaac
     1501 tcggttatgg catcattgca gaaggggtag aaacccttga acagcttagt atactccgta
     1561 gtcttaattg cgaaatcgtg caaggttatc tatttagtca accagtcagt gctgcccagc
     1621 tttcagtgtt actacagagt gataagcaca aaatgattct cgaccaatgc cgggttaaca
     1681 aaatcgctta atttgttaga aattactggg taatctgtaa aattagccaa gctagcaaca
     1741 agaatctaaa atagcagggg tgtttgttaa ttttgctaag caccatcaat gtcgaaaatt
     1801 ttcatcaatt accgtcgcag tgacagcgca ggttatgcag gtcggctcta cgatcgattg
     1861 gttaagcatt tcggggccga tcatctgttt atggatatcg atcagatcga acccggagaa
     1921 gattttgtcg aagtcttaca gaaaaaactg caagcggtac aagttgccgt ggtactgatt
     1981 ggaaaatcct ggcttgatag taaagatgaa accgggcaac ggcgcttaga caatccggat
     2041 gattgggtgc gaatcgaaat tgccacactg ttggaacgga aaattcggat tattccagta
     2101 ttgatcggtg gcgctgccac gcctaaatca tcacaactgc ctgaattatt aatttcatta
     2161 gtaagacgac aggcctttga aatcagtgat actcgctttc attcggatgt cgataagttg
     2221 attcaagcgc tcgaaaaaat tattcaatta caactgactc aattaccccc caagccaaat
     2281 ctgattactc ctgactcatc aatacccgat gatgttagac atcaaaaact aaccgatgcg
     2341 caacattctc cggcccagag tcgctctgaa tctgctagtg ttaataaaaa accgcgatct
     2401 accattagtc ggttcgttat gatcacgatt ggctgtatag ccttggtgtt tacagtattg
     2461 agtattgtta aacttcctat aacagcgcag catgatagta gccgtgatcg tatttcatca
     2521 ccagcaccaa actcggttgt gtctgagtca attcagctga attctgagcc tgtagcatct
     2581 gctagcacgg aagaatcggt accagcagcc ggacagcaag atagcaaccg tgatggcatt
     2641 tcaacgtcac caccagaacg ggttgcgaca gtggcaaccg agcagcatgc tgagcccgca
     2701 gtatcgactc gctctgaaga atcggagcta gcttcgatgt atgattttga accgcaaatg
     2761 gtgcggattt cacccggaag ctttatcatg ggttcaccgg aatctgaagc gggtcgaagt
     2821 gccgatgaag gaccgcagca tccggttaag attacctacc cattcgaaat cggtcgctat
     2881 gaagtcacgt ttgcacaata cgatgcgttt gccaaagata cacaacacca attgcctgat
     2941 gatcgtgggt ggggacgcgg tgatcgaccc gtgatcaatg ttacctggca tgatgcgcaa
     3001 gcctacgtta aatggttgtc agataaaacg ggtaaaaaat atcgtttacc caccgaagcg
     3061 gaatgggaat atgtggcacg cgcgggtacc gccaccgctt attggtgggg cgatcgcatt
     3121 gggaaagcta atgcagcttg tgatggttgc ggtagtcaat gggatggtaa gaaaacgaca
     3181 ctggtaggct cgtttaaacc caatgcgttt ggggtatacg atactgccgg taatgtatgg
     3241 gaatggaccc aggattgctg gtacggtgat tatacgcgtg caccgaccga tgggtctgct
     3301 tggttggagc agaatggcgg cgattgctcg cgccgggtgg ttcgcggcgg gtcttggagc
     3361 agctatcctc agtagtcggt cctgcaaaac gcttaggggc cctataaatg ttcgaattca
     3421 tgcga
//
LOCUS       DAOQLJ010000219         3410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_14799_length_3410_cov_6.679285, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000219 DAOQLJ010000000
VERSION     DAOQLJ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14799_length_3410_cov_6.679285"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..1259)
                     /locus_tag="PK522_11160"
     CDS             complement(294..1259)
                     /locus_tag="PK522_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNC42332.1"
                     /translation="MFLDNSIMSRVMFGVVVFIFSTFAQSASWILPPGDVDLFGQIKT
                     IPANRDETLLDVARRFGIGQDEMMLANPTVDRWLPEEGAEIVLPKRYIIPQGDRTGFI
                     INLPEMRLYYFPKPNKGKKPVVITHPISIGRMDWRTPLGKTSVVRKQKDPTWIPPQSI
                     KDEAIAAGDPPPPPIVLPGPNNPLGRHAIYLGTAGYLIHGTDKPFGIGMRVTHGCLRM
                     YPEDIETLFEKVTVGTPVQLLNQPIKIGWLADSLFVELHPPLDEDEAEYVDYGQKVRD
                     VIASFIEKTTTGKNANHTRKNIEIDQSALELAIFERNGVPILISK"
     gene            complement(1406..1951)
                     /locus_tag="PK522_11165"
     CDS             complement(1406..1951)
                     /locus_tag="PK522_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNC42333.1"
                     /translation="MYNKFILLILGILSSCLVNAHGDVWVDVDTNNLTLSVMRGEKIE
                     LSFDNISIGRYGASKARMMGDNRTPIGTFRIGWIKESRRFHRFFVFDFPNREAADLAL
                     AENRITREIWQSIVNALDTGQSPPQNTPLGGYLGIHGLGRGDQKVHQRYNWTNGCIAL
                     TNAQIDQLSKWLKPGVLVKVW"
     gene            2265..2675
                     /locus_tag="PK522_11170"
     CDS             2265..2675
                     /locus_tag="PK522_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42334.1"
                     /translation="MDILSMLFGLLIGVIVGIWITHIWLAYQRQESTAKLSRLFDQLW
                     QNHFGLMKEMKLDLDNPEYKFQREFFVLNKNKRFNLKRPCLAYFFDDHEGLSDQLGAL
                     SAYGLIREVNGSSDTPAKYQFNEHFVELLRGKQT"
     gene            complement(2747..3178)
                     /gene="dksA"
                     /locus_tag="PK522_11175"
     CDS             complement(2747..3178)
                     /gene="dksA"
                     /locus_tag="PK522_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HNC42335.1"
                     /translation="MSSEKLKSTTILSNYRPKKGELYMNPDQLAHFRKILEGLRIELG
                     QDIDRTVHSMQEEATVFADPNDRASQESDVTIELRNRDRERKLIKKINETIAKIDSGE
                     YGYCESCGIEIGLNRLEARPTASLCIDCKTLEEIREKQLAK"
     gene            complement(3132..>3410)
                     /locus_tag="PK522_11180"
     CDS             complement(3132..>3410)
                     /locus_tag="PK522_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42336.1"
                     /translation="RLIEVFWDDEDVEDQIQEFIDYFNKTHNPPAPIVKSSNPDVVIV
                     IDDLLNQFVSNGNNFNIIDLLFQEYLNLTNDFGDVFRKIEINDNPIKL"
BASE COUNT         1070 a          727 c          578 g         1035 t
ORIGIN      
        1 ctgctataaa gataaataaa tgaggcatct gtgtctcctc caaggtaaat aaattagggt
       61 taacagccat acacaatgcg aataataagc ttgctaactc tatactttaa ataataaaac
      121 cacgacaaca tagcgatgct acatgatttt tttagcaata taaagcaaga agcatatctt
      181 cagtaataaa gtgtgcatcc cctcagcctt aacggcatct ttgtatgaat atgagtaaaa
      241 attattaatt ccatttatga aaatatcgaa atacccgatc aaggaacaca cgactacttt
      301 gagataagta taggaacacc attcctttcg aaaatagcta actctaatgc cgattgatca
      361 atctcaatat ttttgcgtgt gtggttagcg ttttttccag ttgtagtctt ttcaataaag
      421 cttgcaatca catcgcgtac tttctggcca taatcaacat attctgcttc atcttcatcc
      481 aaaggtgggt gcaactctac aaaaagggaa tcagctaacc aaccaatttt tatcggctga
      541 ttcagaagtt gtactggggt tcccaccgtt actttctcaa acaacgtttc aatatcctct
      601 gggtacattc gtaagcaacc atgggttacg cgcataccaa tcccaaaggg tttatcagtg
      661 ccatgtatca aataaccagc agtccccaaa tagatcgcat gacgacccag tggattatta
      721 ggccccggta aaacaatggg tggtggcggt ggatcacctg ctgcaattgc ttcatctttg
      781 attgattgag gagggatcca cgtaggatct ttctgttttc taacaactga tgttttgcca
      841 agcggtgttc tccaatccat ccttccaata ctgatggggt gggtaatcac cactggtttc
      901 ttacctttat tcggcttagg gaaataatag aggcgcattt caggcaagtt gataataaaa
      961 cctgtccgat ctccctgcgg tatgatatac cgtttaggca atacaatttc agcaccttcc
     1021 tcagggagcc agcgatcaac agttggattc gcaagcatca tttcatcctg acctatacca
     1081 aaacgtctcg caacatccaa aagtgtttca tcgcgattag caggaatcgt tttaatttgc
     1141 ccaaataaat ctacatctcc agggggtaat atccatgatg cggattgagc gaaggttgaa
     1201 aagataaaaa ctacaacccc aaacattact ctactcataa tcgaattatc taaaaacata
     1261 tacccacctt tccttcccat tttcttttcc tgaattaaga acaccgagca ttcaagctgc
     1321 actaaatttg ataacttggc taatttaaat taagtcaacg ataagaaatc gatttaaaaa
     1381 tatgcctcta accactacca gttttctacc aaaccttcac tagcacacca ggtttgagcc
     1441 atttactcaa ctgatcaatc tgtgcattgg ttagcgcaat acaaccgtta gtccaattgt
     1501 atcgttgatg aaccttttga tcgccccgtc cgagaccatg gatacctaaa taaccgccca
     1561 gaggagtatt ctgcggagga gattgtccag tatcaagtgc gttcactatt gactgccaga
     1621 tttctcttgt aatgcggttc tctgctaaag ccagatccgc tgcttctcga ttaggaaagt
     1681 caaaaacaaa aaaacgatgg aagcgacgcg actctttaat ccatccaatc cggaacgtac
     1741 caattggggt ccggttgtcc cccatcattc tggccttgct tgctccgtag cgtccaattg
     1801 aaatgttatc aaaactcagt tcaattttct caccccgcat tactgacaaa gtcaaattat
     1861 tggtatcaac atcaacccaa acatctccat gtgcatttac caagcagctt gataatatgc
     1921 ctaaaatcaa aagaatgaac ttattgtaca tagcttatta gtagatttat ctaatagtaa
     1981 atcgaacaaa aaacatcgta attagaccac ttagttagaa acatcaattc actgagataa
     2041 agtttttata ccgctattta atagataatg cagctaaact agtagcatct gctaatatct
     2101 gttagcgtaa accacatata tcagagtgct agtatctacg ttaatctagc agaattcctg
     2161 gtaacataaa ttgcatttat ttataaaata gcttttggcg tattatatcc acacgcgcaa
     2221 ggtcatctaa tttttaaaac aataacagag ggggagaaat aataatggat atcctaagca
     2281 tgctctttgg cttattaatt ggcgttatcg tcggtatttg gatcactcat atttggctag
     2341 cctatcaacg tcaggaatcc acagcgaagc tgtcgcgact gtttgatcaa ttatggcaaa
     2401 atcacttcgg cctcatgaaa gaaatgaaac tcgatctaga taatccagaa tataaattcc
     2461 agcgagaatt ctttgtctta aataaaaaca agcgttttaa ccttaaaaga ccatgtttag
     2521 catatttctt cgatgatcat gaagggttaa gtgatcaatt aggtgcctta tcagcttatg
     2581 gattgatcag agaagtaaat ggatcaagcg ataccccggc taaatatcaa tttaacgagc
     2641 attttgttga attgcttcgc ggcaaacaaa cataataaaa tacattgcct gattaaaaaa
     2701 agaacaaaac tagcctatta gcgttatttt tgttcttttt attatcttac ttagcaagtt
     2761 gcttttctct tatctcctct agcgttttac agtcaataca taacgaagct gtagggcgcg
     2821 cttctagtcg atttaatcca atttcaatac cacagctctc acaataacca tactcgccag
     2881 aatcaatttt agcgattgtc tcattaatct tcttgataag ctttctctcc ctatcgcgat
     2941 tacgcaactc aatcgtaaca tcggattctt gactagccct atcattagga tctgcaaaaa
     3001 cggttgcttc ttcctgcatc gagtgaacag tacgatctat atcttggcct aattctatcc
     3061 tgaggccctc taaaatcttt ctaaaatggg ccaattgatc aggattcata taaagctctc
     3121 ctttctttgg cctataattt gataggattg tcgttgattt caatttttct gaagacatct
     3181 ccaaaatcgt ttgtcaagtt gagatattcc tgaaagagga gatcaatgat gttaaaatta
     3241 tttccattcg agacaaattg attaagcagg tcgtcaataa ctattactac atccggatta
     3301 gaagatttta caatcggtgc agggggatta tgcgttttat taaaataatc aataaattcc
     3361 tgaatttggt cctctacatc ttcatcgtcc caaaaaacct caatcagtct
//
LOCUS       DAOQLJ010000220         3364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_15155_length_3364_cov_7.014808, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000220 DAOQLJ010000000
VERSION     DAOQLJ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15155_length_3364_cov_7.014808"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="PK522_11185"
     CDS             <1..1068
                     /locus_tag="PK522_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC42337.1"
                     /translation="RTNIESLKSLNAQSLVRSNTGGSSGEPLIFYLGKERVSHDVAAK
                     WRATRWWNVDIGDPEIVIWGSPIELGTQDNIRALRDSLFRSKLLPAFEMSDQKLQVFL
                     DEIRSFQPKILFGYPSALSFIARYAEKTGEQMNDLGIKVAFVTSERLYDEQRQQISKT
                     FNCPVANGYGGRDAGFIAHECPAGGMHITAEDVIVEILDNQGIPVPCGESGEIVITHL
                     ASSDFPFIRYRTGDIGKLDDQLCSCGRGLPLLKEIEGRSTDFVVAQDGTVMHGLALIY
                     ILRKIPEINAFKIIQENLDLTRILIVSKSALDAATIKLITNEFQTRLGNDITIKIEEV
                     NEIPLEKSGKFRYVISKVIPP"
     gene            1068..2018
                     /locus_tag="PK522_11190"
     CDS             1068..2018
                     /locus_tag="PK522_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC42338.1"
                     /translation="MEQLLLEPTQDLSMRFNRIAVNTISEKNNNAQTVRYIAFTDWGN
                     SENTQVVICAHGLTRNGRDFDYLAKALQDQFRVICIDIVGRGQSDWLTNAEEYNQPAI
                     YLSDIKILLTHILAQYEQPVHLNWVGISMGGLIGMILASQDLPIPIKSIIMSDIGPRI
                     PIDAILRFKRYVGEDPKFTDIDQLENYIRVISAPFGELTKDQWRHLTLYSACKYSEGV
                     YGFRYDPRIAINFHESFEHDIDLWSFWDQLTIPALVLHGEKSDVLLPQIAEEMRIRGP
                     RAKIIKISNVGHAPLLVNEEQISHVKGFLLKQIQLHKNTK"
     gene            2206..2490
                     /locus_tag="PK522_11195"
     CDS             2206..2490
                     /locus_tag="PK522_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNC42339.1"
                     /translation="MKLFKKIPNPREVRQKLNMNQQEFWSKVGVTQSGGSRYESGREM
                     PKAVRELVRLVHIDRIDLLKIKRDDILIAAMLKTQHPELYKDLKKSIKMR"
     gene            complement(2559..3044)
                     /locus_tag="PK522_11200"
     CDS             complement(2559..3044)
                     /locus_tag="PK522_11200"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNC42340.1"
                     /translation="MVGKRYFREFMNYLVFVILVTLLSFSALSFASNESDKENVLGLW
                     LTKKKDVVVQIKYCEEKTLCGYVSWISPEEMSEKNATLCGRKVLWDARLADDQPIRWV
                     GGVLYKADEDKYYSANLQLIDADTLALRAYIGVPLLGKTKKLTRTSEKVHPSCIVPNQ
                     P"
     gene            3164..>3364
                     /locus_tag="PK522_11205"
     CDS             3164..>3364
                     /locus_tag="PK522_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-alkenal reductase"
                     /protein_id="HNC42341.1"
                     /translation="MLSNLTPPTRPDPFARRMVIIALAIAALMLLWRTLPLIESLFVP
                     EKGEIRTVTARGDLALDERTTIE"
BASE COUNT         1088 a          664 c          621 g          991 t
ORIGIN      
        1 aggaccaata tcgaatcatt aaaatccctt aatgctcaat cacttgttcg atcaaacaca
       61 ggcggatcta gcggagaacc cttaattttt tatttaggta aagaacgggt aagccatgat
      121 gttgcagcaa aatggcgagc tacccgatgg tggaatgttg atatcggtga tcctgagatc
      181 gtaatttggg gatctcctat cgaattagga acccaggata atatccgcgc gttacgagac
      241 tcattatttc ggagcaaatt actgcctgct tttgaaatgt cagatcagaa attacaagtt
      301 ttccttgatg aaattcgttc ttttcagcct aaaatactct ttggctatcc ttcagcccta
      361 tcgtttatcg cgcgttatgc tgaaaaaacc ggcgaacaaa tgaatgatct cggaataaaa
      421 gttgcatttg taacttctga gcgtttgtac gatgaacagc gccagcagat cagcaaaacg
      481 tttaattgcc ctgttgccaa tggctatggg gggcgcgatg ctggatttat tgcgcatgaa
      541 tgcccagcag gtggtatgca tattactgct gaggatgtca ttgttgaaat ccttgataac
      601 caaggaatac ctgtaccgtg tggtgaatcc ggtgaaatcg tcattacaca tcttgcaagt
      661 agcgactttc cattcatacg ctaccgaacc ggcgatattg gcaagctcga tgatcagtta
      721 tgtagctgcg ggagaggtct accactatta aaagaaatcg aggggcgaag cactgatttt
      781 gttgttgctc aagatggtac cgtgatgcat ggacttgctt tgatttatat attaagaaag
      841 attcctgaaa ttaatgcatt caagataata caagaaaacc tcgatttaac acgtatatta
      901 attgtgagta aatctgcact agatgcggca acaataaagt taattaccaa tgaatttcaa
      961 acaagattag gcaacgatat tacgattaaa atagaagagg ttaatgaaat tcctttagag
     1021 aaatctggga aatttcgcta cgtcatcagt aaagtaatac caccttaatg gagcagttat
     1081 tactagaacc cactcaagat ttatctatgc gctttaatcg aattgctgtt aatacgattt
     1141 ctgaaaaaaa taataatgct cagacagttc gctatatcgc gtttactgat tggggaaatt
     1201 cggaaaatac acaggttgtt atctgcgctc acggacttac tcgaaatggt cgggattttg
     1261 attatcttgc aaaagcttta caagaccagt ttagagttat ctgcattgac atcgtcggtc
     1321 gcggacaaag tgattggctt acgaatgcag aagaatacaa tcaaccagcg atctacctat
     1381 ctgatataaa aatcttacta acccatatcc tagctcagta tgagcaaccc gttcatctaa
     1441 actgggtagg catatcaatg ggggggctta ttggtatgat acttgccagc caggatttac
     1501 caattcccat aaaatcgatt ataatgagcg acatagggcc tcgaattccc attgatgcca
     1561 ttctccgctt taagcgctat gttggtgaag atcctaaatt cactgatatc gatcagctag
     1621 aaaactatat ccgagttatt tcagcgccat ttggagagtt aactaaagat cagtggcgcc
     1681 atctgacact gtatagtgcc tgcaaatatt ctgaaggcgt gtatggattt cgttacgatc
     1741 cccgtattgc tatcaatttt catgaaagct ttgagcatga tattgattta tggtcattct
     1801 gggatcagtt aactattcct gccttggtac tgcacggtga aaaatcagat gttttacttc
     1861 cccaaattgc tgaagaaatg agaattcgtg gcccaagagc aaaaataatt aaaatatcaa
     1921 atgtgggaca tgccccctta ttagtcaacg aggaacagat ctcacacgtt aaaggatttt
     1981 tattaaaaca aattcaattg cataaaaaca ctaaatagat ttaaagaaat aacgcataaa
     2041 ttccttttaa ataaagctta aattccatat agatactccg ataaaaatca aactaattct
     2101 gaatgaatat caccctgagt tagtattttt ttgatctatc tgaaattcga acaaaaaaat
     2161 tatttctaag tctaatcgct ttgatgacaa ttgagagaaa acattatgaa attattcaaa
     2221 aaaattccaa acccaagaga agtaaggcag aagctaaata tgaatcagca agaattctgg
     2281 agtaaagttg gcgttactca gagtggtggt tctcgttatg aaagtggcag agaaatgcca
     2341 aaagccgtca gagaacttgt aagactcgtg catatcgatc gtattgattt actcaaaatt
     2401 aaaagagatg acatactgat cgctgcaatg cttaaaacac aacatccaga gttatataaa
     2461 gatctgaaaa aatctatcaa aatgcggtag tttcttcatg aatcaacaag gtctgccttt
     2521 gttttaatgt caaagcagca tgacgcttac tacctgtttt atggttgatt aggaacgata
     2581 cacgaagggt gtactttctc tgaagtacgg gtaagttttt tggttttccc caataaaggc
     2641 accccaatat aagcacgaag tgctaatgta tcagcatcaa ttaattgcaa gttagcactg
     2701 tagtatttat cctcatctgc tttatacaaa acaccaccaa cccaccttat cggctgatca
     2761 tctgctaaac gtgcatccca caacaccttt cttccacata atgtagcatt cttctcgctc
     2821 atctcttccg gactaatcca agacacatac ccacataaag tcttttcttc acagtatttg
     2881 atttgtacca caacatcttt cttcttcgtt aaccataatc cgagaacatt ttctttatct
     2941 gactcattag atgcgaaaga taaagcagaa aaagaaagca gcgtcaccaa tattacaaaa
     3001 accaaataat tcataaattc tctaaaatag cgttttccta ccataaatca cttatttaaa
     3061 taatcgtatt ctaaagtgat gtacattcta tcttgtttta gatcataagg tttattatta
     3121 gcatcccata accaattttt gtatctactt acttttagtc aaaatgcttt ccaatctaac
     3181 gccaccaaca cgaccagatc cgtttgctcg ccgtatggta atcatcgcac tagcgatcgc
     3241 agcactaatg ctactttggc gcactttacc actcattgag tccctcttcg ttccagaaaa
     3301 gggggaaatc agaacagtaa ctgcaagagg agatcttgct ctagatgaac gcactacaat
     3361 tgaa
//
LOCUS       DAOQLJ010000221         3346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_15280_length_3346_cov_5.191431, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000221 DAOQLJ010000000
VERSION     DAOQLJ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15280_length_3346_cov_5.191431"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            391..1545
                     /locus_tag="PK522_11210"
     CDS             391..1545
                     /locus_tag="PK522_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNC42342.1"
                     /translation="MAFKGQITHLSQKGLGVVQNKQDGIAYFVSGTWPGDHGEFEIID
                     RTLPNKKYGYAKLVQLIQPSVDRQQAPCPFIGTTETACAGCPWMIASYTSQLEQKRNR
                     FKYALQRAGFNLESLSIPPVHPSPVQYGYRNRFQLKTDGNQLGFVAEGSHRIAPIQDC
                     LVLNEACKTLLHKLHNQLPRSDWQPIKGANWNFIDLDDDLSFEQIRLNHKRPFKQGNC
                     AQNAWIKAWLSSKLAANPVPQKIIELFCGSGNFTEILAASGCRSIISYEAELDAIQQL
                     KAKHLPNVDAQSIDLFKPFVWKTIKKVISDADTLVLDPPRAGLKNMKGFFEHFLALRT
                     IYYISCDPESFARDAWHFTKKGWKLAEIQLIDLFPHTPHVETLAILKKQQ"
     gene            1668..2714
                     /locus_tag="PK522_11215"
     CDS             1668..2714
                     /locus_tag="PK522_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNC42343.1"
                     /translation="MKTDTIQITPELLVLIAEIDEFKGAWRALGTLAPERLKALRHIA
                     TIESIGSSTRIEGSKLTNREAEQLLGSLEIKRFSSRDEQEIVGYAEVMETVFYVWADI
                     PITENHIKQLHRDLLRHSDKDERHRGEYKTLRNDAGAFDAAGNMIGIVFETATPFDTP
                     RRMQELVTWLNEARERKHLHSLLIVAVFIVTFLEIHPFQDGNGRLSRILTTLLLLQAG
                     YAYVPYSSLESAIENSKEGYYLALRQTQGTIRTETPNWQPWLVFFIRALQQQKSHLAL
                     KMEREKNTLAVLPALAVKILDYVRDHGRVTTRDMVRETGTSPNTLKATFTSLVEKGFL
                     VRRGGGRSTWYALP"
     gene            2838..>3346
                     /locus_tag="PK522_11220"
     CDS             2838..>3346
                     /locus_tag="PK522_11220"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC42344.1"
                     /translation="MKAQKEELAIVLGQFNPWWRGESIPDLPDWHRASFGELMQWIMD
                     PPAARAIFLSGARQVGKTTLLLQAINHLLAQGIPASNLLYATFDHPIIKLAGADAVIQ
                     TWREREPKREGQEFIFLDEAQFINEWGTWIKHQVDFFKQRRIIFTGSAMPILQADQES
                     GVGRWHSIRL"
BASE COUNT          965 a          857 c          691 g          833 t
ORIGIN      
        1 gctaaataca ttaacccaag gatctggttc agggtgagga acaacaggaa ttccccaaga
       61 tccaggataa ggcgttccac accagccccc ggaaaccgct tgtaatgcgt caatatccaa
      121 caccgtcatt ccacccattt tttcacgact attcacttca tttgacatga tcattctcct
      181 aaaaaattaa atcgattaag tgattatctg caaaataaca atccactttg cactcaaccc
      241 agtcctacca taactttcat tcacaaaagc atcagtgtgt aaaatcacgc tggcgaagct
      301 tgatcaggtc taaaaaccga cttgaagcat tattgagctt atgagaccat gcgcctcaaa
      361 atcgcatcga caaagaggaa cgagtagcac atggcattta agggtcaaat cacgcatctc
      421 tcacaaaaag gcctgggagt cgttcaaaac aaacaagatg gcattgctta ttttgtttca
      481 ggcacctggc ccggggatca cggtgaattc gaaattatcg accgcactct cccaaataag
      541 aaatatggtt acgcaaaact cgttcaactg attcagcctt ctgtagaccg tcagcaagcc
      601 ccttgcccat ttatagggac gacagaaacc gcctgcgcag gatgtccatg gatgattgca
      661 tcttatacaa gtcaactcga acaaaagcgg aatcgcttta aatacgccct gcaacgcgct
      721 ggattcaacc tcgaatcgct atcgattccc cccgttcatc cttcacccgt gcaatatggc
      781 taccgtaatc gcttccaact caaaacggat ggtaatcaac ttggcttcgt tgcagaaggc
      841 tcccaccgga ttgcaccgat tcaggattgc ctcgttctca atgaagcttg caaaacgtta
      901 ttgcataaac tgcacaacca attacccaga agcgattggc agccgattaa aggcgctaat
      961 tggaacttta ttgacttaga cgatgatcta tcctttgaac agatccgcct caaccacaag
     1021 cgccctttca aacaaggtaa ttgcgcacaa aacgcctgga tcaaagcttg gctcagcagt
     1081 aaattagcag caaaccctgt tccgcaaaaa atcatcgaac tcttttgtgg ctccggtaat
     1141 ttcactgaaa tccttgcggc ttcgggctgc cgctcgatca tttcctatga agcggaattg
     1201 gacgcgattc agcaactcaa agccaaacat ctacccaatg ttgacgcaca gtcgatcgat
     1261 ctattcaagc ccttcgtttg gaaaaccatc aaaaaagtaa tcagcgatgc agatactctc
     1321 gtgctcgatc ctccccgcgc tgggctcaaa aatatgaaag gcttttttga acactttctc
     1381 gctttacgaa cgatctacta catctcgtgc gacccagaaa gctttgcacg cgacgcctgg
     1441 catttcacca agaaaggctg gaaactcgct gaaattcaac tgattgatct gtttccgcac
     1501 acccctcatg tagaaacact cgctatattg aaaaaacagc agtgattttt tacaaaaaaa
     1561 ctcagttata gacattccgc agcaagctgt atcgttatat tttcaggtat aaatcggtat
     1621 ttatttcggt agacaatccg gtataatccg gcatatattc cggtattatg aagaccgata
     1681 ccattcagat cacgccagaa ctcttggttc tgattgctga gattgatgag ttcaaaggtg
     1741 catggcgcgc ccttggcacc cttgcaccgg aacgactaaa agcattacgt cacattgcca
     1801 ccattgagag cattggttct tccacccgca tcgaaggtag taagttgacc aaccgcgaag
     1861 cagaacaatt attgggcagt ttggaaatca aacgcttcag cagtcgcgat gaacaagaaa
     1921 tcgtcggcta tgctgaagtg atggagaccg tcttctacgt gtgggccgat attccaatta
     1981 ccgagaacca tatcaaacag cttcaccgtg atcttttgcg ccacagcgac aaagacgagc
     2041 gacaccgtgg tgaatacaaa acgttgcgca atgacgcggg agccttcgac gctgctggca
     2101 acatgatcgg tatcgtcttc gaaacagcca caccattcga cacgccaagg agaatgcagg
     2161 aactcgtcac ctggctgaat gaagcccgcg aacggaagca ccttcactcg ctgctgatcg
     2221 ttgctgtctt catcgtcacc ttcctggaaa tccatccctt ccaagatggc aatggccgtt
     2281 tgagtcgcat cctaaccacg ctactcctgt tgcaggctgg ttatgcctat gtaccgtatt
     2341 cgtcacttga aagcgcgatc gaaaacagta aggaaggata ttacctcgcc ttgcgccaaa
     2401 cccaaggtac catccgcaca gaaaccccca attggcagcc ctggcttgtt tttttcatac
     2461 gtgctttgca acagcaaaaa tcccacctag ctttgaaaat ggagcgtgag aaaaatacac
     2521 ttgctgtttt acccgcgctc gctgttaaaa tcttagacta tgtccgcgac catggtcgcg
     2581 ttaccacccg tgatatggtg cgcgaaacag gaacaagtcc caatacgctc aaagcaacct
     2641 tcacatcgtt agtcgaaaag ggatttctag tacgtcgcgg tggtggtcgc tcaacttggt
     2701 atgccctgcc ctgatttaga atttccccac gcgcgctatt gaaaaaatag cagtaatttt
     2761 tcacaaaaaa tcattaaaaa tgatttattt gtatagtata gtttaactat atgatgaatt
     2821 taagctaaaa atacgtaatg aaagcacaaa aagaagagct tgcgatcgtt ctagggcaat
     2881 tcaatccttg gtggcgtggt gaatcgattc cagatttacc agactggcac cgcgcttcat
     2941 tcggtgaatt aatgcaatgg attatggatc cgcctgctgc gcgtgctatc tttttatcgg
     3001 gagcacgtca ggtaggtaaa accacgctac tgctccaagc aattaatcac ctcctagcgc
     3061 aaggtattcc agctagcaac cttttatacg cgacctttga tcaccccatt atcaaattag
     3121 caggcgctga cgcagttatt caaacctggc gagagcgtga acccaaacga gaagggcagg
     3181 aattcatctt tttggatgaa gcgcaattta ttaatgaatg ggggacttgg atcaagcatc
     3241 aagtcgattt ctttaaacaa cggcgcatca ttttcactgg atctgccatg ccaatcttac
     3301 aagccgatca agaatctggc gttggtcgct ggcacagcat ccgcct
//
LOCUS       DAOQLJ010000222         3329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_15423_length_3329_cov_5.273366, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000222 DAOQLJ010000000
VERSION     DAOQLJ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15423_length_3329_cov_5.273366"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PK522_11225"
     CDS             <1..735
                     /locus_tag="PK522_11225"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNC42345.1"
                     /translation="HMGNTAENIAAKWNIGREKQDQFALESHRRATQAIRNGYFTSQI
                     LPIEISSRGKVVVFDTDEHPRDLIDLEKLSNLPSVFQENGTVTAGNASGINDGAAAVV
                     LCDATTAESDHLQPIARLVAYGYAGVDPRYMGIGPVPAVHQALHRACLTIDDMDVIES
                     NEAFAVQACAVACELGFDPIKTNPNGGAVAFGHPIGATGCVLTIKALYELHRIKGRYA
                     LVTMCIGGGQGIAAIFERLPSFAQLN"
     gene            755..2401
                     /gene="pgi"
                     /locus_tag="PK522_11230"
     CDS             755..2401
                     /gene="pgi"
                     /locus_tag="PK522_11230"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNC42346.1"
                     /translation="MTSLTQSLVWLALKAHQSKLESQHLRSLFAEDPHRFEKFSIRFN
                     DFLLDFSKQPVTNETMNLLFQLATQQKLSDWITQMFQGEKINSTEHRAALHIALRANQ
                     TVTTESVDILPAVKQVLQQMESFTQAVHQGIHRGYSGLTFTDVVNIGIGGSDLGPVMV
                     TEALKPYWNGQITPHFVSNIDGAHLAETLKALNPATTLFVIASKTFTTLETIANAHAA
                     RTWFLTQGGSEKEIARHFVAVSTNQKAVQQFGIDPANMFVFWDWVGGRYSLWSAIGLS
                     IALTLGMQHFYDLLSGARQMDQHFLTTPFAENLPVILGLIGIWQINFLGATSHAILPY
                     DQSLHRFPAYLQQLEMESNGKRVTRDGTRVDYQTGTVVWGESGTNGQHAFYQLLHQGT
                     PTVTADFLAPCQSHYAVDHHHQLLLANFFAQTEALMLGKTADEVQVELQSQGYTLSEI
                     EQLAPHRVFAGNHATTSILFKKLDPKTLGSLIALYEHKVFVQSVIWNINPFDQWGVEL
                     GKQLANKIMTDLKTDQKVTAHDASTNGLINYFKENSDISN"
     gene            2435..>3329
                     /locus_tag="PK522_11235"
     CDS             2435..>3329
                     /locus_tag="PK522_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNC42347.1"
                     /translation="MSTRLSKHALKVLFVTSEVYPLCKTGGLGDVSAALPASLREQQV
                     DVRILIPGYPQVLAGLKYKRKVAEFNDLAHFPPAKLLSANLQVNASTSIPVFVIDCPS
                     LYQRTGGPYLDVAGHDWPDNAQRFGLLSKMGALLGSDASPLTWQPNVVHCNDWQSGLT
                     PAYLHFHRGLKSPTLMSIHNLAFQGCFAPELVSQLGLPSESYSVQGVEYYGSLSFIKA
                     GLYYADHISTVSPSYAHEIQQERLGFGLHGLLSARKEQISGIVNGIDTIEWDPSADIH
                     IAKKYTIRRLSNKQANKLAIQQ"
BASE COUNT          920 a          702 c          763 g          944 t
ORIGIN      
        1 catatgggaa atacagccga aaacatcgcg gcaaaatgga atattggacg tgagaaacaa
       61 gatcagtttg cgttggaaag ccatcgacgt gcgacacagg ccattcgtaa cggttacttt
      121 acctcgcaaa ttttaccgat tgaaatatca tcgcgtggca aagtggttgt ttttgatacc
      181 gatgaacatc cccgtgactt gatcgatcta gaaaaattat caaacttacc tagtgttttc
      241 caggaaaatg gcacggttac cgcaggtaat gcgtcaggta tcaacgatgg cgcagcggct
      301 gttgtgcttt gtgatgccac aactgcagaa agcgatcact tgcaacccat tgcacgatta
      361 gtagcctatg gctatgccgg tgttgaccct cggtatatgg gtataggccc tgttccagca
      421 gtacatcaag cactacaccg tgcttgttta acgattgatg atatggatgt tatcgaatcc
      481 aatgaagctt ttgctgtaca ggcctgcgca gttgcttgtg agctcggttt cgatccgatc
      541 aaaaccaatc ccaatggcgg cgctgttgct ttcggacatc caatcggtgc tacgggatgt
      601 gttctgacga tcaaagccct ttatgaactg catcgtatca aggggcgcta tgcattagtg
      661 acgatgtgca tcggcggtgg gcaaggcatt gcagctattt ttgaacgatt accctcgttt
      721 gcacagttaa attagaaagg tagatgccac gatgatcaca tccttgaccc aatcactcgt
      781 atggcttgct ttaaaagcac atcaatctaa gcttgaatcg cagcatttac gtagcctgtt
      841 tgcagaagat ccacaccgtt tcgaaaaatt ctcgattcgt tttaatgatt tcttgcttga
      901 ttttagtaag caacctgtaa cgaatgaaac catgaattta ttgtttcagc ttgcgacaca
      961 gcaaaaattg agtgattgga tcacacaaat gtttcaaggg gagaaaatca attccactga
     1021 gcatcgagcg gccctgcata ttgcattgcg ggctaatcaa acagttacga ctgaaagtgt
     1081 ggatattttg ccagcggtaa agcaagtttt gcagcaaatg gaaagcttta cacaagcagt
     1141 tcatcagggt attcatcgtg gttactcagg gttaactttt accgatgtgg tcaatattgg
     1201 tattggtggt tctgatcttg ggccagtcat ggtcacagaa gcgttaaagc cttactggaa
     1261 tgggcaaata acgcctcatt tcgtatcgaa tattgatggg gcacatcttg ccgaaacact
     1321 aaaagcgtta aatccagcaa cgacgttatt cgtaatcgct tcaaaaacct ttacgacact
     1381 agaaactatt gctaacgccc atgcagcgcg tacctggttt ttgacacagg gcggttctga
     1441 aaaggagatt gcacgtcatt ttgtagcagt ttcaacgaat caaaaagcgg tccaacaatt
     1501 tggaatcgat cctgccaata tgtttgtttt ctgggattgg gtcggggggc gttattcact
     1561 gtggtctgcg attggattat cgattgcctt gaccctgggt atgcagcatt tttatgactt
     1621 gttatcaggt gcgaggcaaa tggatcaaca tttcctgaca acgcctttcg ctgaaaatct
     1681 tccggtaatt ttgggtttaa tcggaatttg gcagattaac tttctgggcg caactagcca
     1741 cgcaatttta ccgtacgatc agtcactgca tcgatttcca gcctatctac agcaacttga
     1801 aatggaaagt aatggtaaac gtgttacccg agatggaaca cgagttgact accaaactgg
     1861 taccgttgtt tggggggaat cgggaactaa cggccagcat gctttttatc agctactcca
     1921 ccaaggaaca ccgactgtaa cggcggattt cttagcacct tgccagagtc attatgccgt
     1981 ggatcaccat catcaattat tattagctaa tttctttgca caaacagaag cattaatgct
     2041 tggcaagact gctgacgaag tgcaagtaga attacaatcg caaggttata cgctttctga
     2101 aattgaacaa ttggcaccgc atcgggtttt tgcggggaat catgcgacga cgtcgattct
     2161 attcaaaaaa cttgatccga aaacattagg ttcgttaatt gcgctctatg aacacaaagt
     2221 tttcgtgcaa agtgtcattt ggaacatcaa tccatttgat caatggggtg tagaattagg
     2281 aaagcagctt gctaacaaga ttatgactga tttgaaaacc gaccaaaaag ttactgctca
     2341 tgatgcatca accaatggat taatcaatta tttcaaagaa aatagcgaca tctcgaattg
     2401 aaatcgttga tagatcaata catttaataa ataaatgtct acccgtctct cgaagcatgc
     2461 gctaaaagtc ctttttgtta cgtcggaagt ttatccgtta tgcaaaactg ggggactagg
     2521 tgatgtgagt gcggcattac cggcgtcgtt acgtgaacaa caagttgatg tgcgcatcct
     2581 cattcctggt tatccgcaag tgcttgcagg attaaaatat aaacgaaagg tagctgaatt
     2641 caatgattta gctcattttc cccctgctaa gttattatca gccaacctgc aagtcaatgc
     2701 ctcaacaagt atcccagttt ttgtcatcga ttgtccttcg ttatatcaaa gaacgggagg
     2761 gccctattta gatgtggctg gacacgattg gcccgataat gcacagcgtt ttggcttgct
     2821 ctcaaaaatg ggtgcattgc ttggcagtga tgccagtcca ttgacgtggc aacccaatgt
     2881 tgttcactgc aatgattggc aaagtggctt gacgcccgct taccttcatt ttcatcgggg
     2941 tttgaaatcg cctaccctaa tgtcaattca taacttggca ttccagggtt gtttcgcacc
     3001 cgagctggtg agccagcttg ggctaccttc tgaaagttat agcgtccaag gtgtcgagta
     3061 ttatggaagt ttgtcattta ttaaagcagg gctttattat gcggatcata tttcaacggt
     3121 tagccctagc tatgcgcacg agatccagca ggaacgactc gggtttgggt tgcatggttt
     3181 attatctgct cgcaaagagc agatcagtgg gattgtgaat gggattgaca ccatcgagtg
     3241 ggatccatcc gctgatattc atatcgctaa aaagtatacc attcgtcgtt tatccaataa
     3301 gcaagccaat aaactggcga ttcagcaaa
//
LOCUS       DAOQLJ010000223         3325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_15444_length_3325_cov_6.271560, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000223 DAOQLJ010000000
VERSION     DAOQLJ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15444_length_3325_cov_6.271560"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="PK522_11240"
     CDS             complement(<1..1154)
                     /locus_tag="PK522_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42348.1"
                     /translation="MKLLDRITLESVDIAQLHGVCIIPQQYLLLEDWSYNSHYTVPKG
                     TVLCNDDSSMLHMDGHKLLLVKQELSDNIHAAITQIAATTRDYLSSSTRGSWPSPLIP
                     RQVFEEASVLSPVEEMLDVVMDAGHLHTISNRPRIDMRYEEALMPISRAKKIANNAHR
                     HLAMHSETWQQRTLTGIYPKKVMARLSEDEWQLYENKVYARLLDKLYRFLKHRRAELI
                     EQKQNIEDGLNLEGGEGLYYKLADVLYKLWGENWRNSSANNTLEQLEETMKSLNALID
                     KLSLLRQAGIYLKIPKAAQVPDQLHMTNILSHDQHYRHIARLWNSLYSVKARNYTAAE
                     KLEYMLNLQKDYSIFSLLIICRAFESLGFNFSVKEKDTFIDKYTEANAMI"
     gene            complement(1151..>3325)
                     /locus_tag="PK522_11245"
     CDS             complement(1151..>3325)
                     /locus_tag="PK522_11245"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC42349.1"
                     /translation="TYDTKSHEFSTQKIDLEKQQTALLREQVSLSRQQSAVQRQQSEV
                     DKLKSEIEQKQRELEVSLLQLEEQKAELEKLKQAAEMDFALEKQIAIKEKRQELEQLH
                     KQHLSDLKSSSDSLEQKKKELLERERLLIERESQAEAGFLDKQQHLDQLTQQKQASLS
                     ILEHRLKKQRQEQTVWEAAKEEEIEQNYQLKLQELQTKLIHTENNKKQAFEKINQLTE
                     ELSQLEDLKRELAERNVTSLQAEMERLRLENRRLKEDLAQREEDGLLEDNQKLCKKLS
                     YIEEKLQDTEFQLSEANAQRDTLRMSAQEKYNLLREKRVLHEHNRLLDISIEQLKLQI
                     DDLSARQQGELPFPALSKMDQQFQHPARGLQAVPQDLAQFAKAMQVGIAQQELFYSLK
                     DIRLFIAGLAMSKLHLLQGMSGTGKTSLARAFAKAINNVTTGDQEKLYCSMVRVQAGW
                     RDREDLLGHFNAFEKKFYEREALQAIYRAQQPQFSDTVQIILLDEMNLSQPEQYFAEF
                     LSLMETPDLSEIALLESPNEKSPRYFSDQRAIKVPQNVWFIGTANHDETTKEFADKTY
                     DRAHVMEIKRSEEKVSANGYRPVSYSFKSLQEQFDVVCQNEAQKITDIFKKLNGSELM
                     LALEDIKIGWGNRLESHAKKFIPVYQATGGLLSEALDHLLATKLFRTGKVTGRYDTTI
                     EQLQNIESALETSWKELGLDGKPNYSLQLLRDDIQRKEQGGV"
BASE COUNT          866 a          701 c          607 g         1151 t
ORIGIN      
        1 attatcattg cattggcttc tgtgtatttg tcaataaaag tatctttttc ttttacagaa
       61 aaattaaatc ctagactttc aaatgctcta caaattatga gaagtgaaaa tatggagtaa
      121 tctttttgta agtttagcat atactccaat ttctcagccg cggtatagtt tcgcgcttta
      181 actgaataaa ggctattcca taaacgggca atatgtcggt aatgctggtc atgacttaaa
      241 atattagtca tatgtaattg atctggtact tgcgctgctt tcggtatttt cagataaatg
      301 ccggcctgcc gtaaaaggct caatttatca atcaaagcat tgagtgattt cattgtttct
      361 tctaactgct ctaacgtatt attagcagaa gagtttctcc agttctcacc ccacaactta
      421 taaagcacat cagcaagctt ataatataaa ccttcccctc cctctaaatt taaaccatct
      481 tcaatattct gtttttgttc aataagctct gctctacgat gctttaaaaa tcgatataac
      541 ttatctaata agcgagcata tactttattt tcgtatagct gccattcgtc ttcgctgagg
      601 cgagccatta cttttttggg gtaaatacca gtcaatgtac gctgttgcca tgtttcagag
      661 tgcattgcta aatgtcgatg tgcattattt gcaattttct tagcacgact aataggcatc
      721 aaagcctctt catacctcat atctatacgt gggcgatttg aaatcgtatg caaatgcccc
      781 gcatccatga caacatctaa catctcttca acaggactaa gtacactagc ctcttcaaat
      841 acctgccttg gaatcagtgg tgaaggccaa cttccgcgag ttgaactgct aaggtagtct
      901 cttgtagttg ctgcaatttg agtaattgca gcatgaatgt tatcactcaa ttcttgtttg
      961 acgagtaaaa gtttatgccc atccatatgt agcattgaag agtcatcgtt gcataacacc
     1021 gtcccttttg gcacagtata atggctatta tacgaccaat cctccagcaa gaggtattgc
     1081 tgaggaatga tgcaaactcc gtgaagctgt gcaatatcta ctgattctaa cgttattcta
     1141 tcaagtagct tcatacgcca ccttgttctt tacgctgaat atcatcacga agaagttgta
     1201 gggagtaatt aggtttaccg tccaaaccta attctttcca acttgtttct agtgcgcttt
     1261 caatattctg taattgctca atggtggtat cataacgtcc agttactttg ccagtgcgga
     1321 acaatttcgt ggctaacaaa tgatcaagag cctcacttaa taaaccacct gttgcttggt
     1381 acacaggaat aaactttttt gcgtggctct ctaaacgatt accccagcca atttttatat
     1441 cttccagtgc aagcatcagc tcactgccat tgagtttttt gaatatgtct gtaatctttt
     1501 gagcttcatt ttgacagaca acatcaaatt gctcttgcaa gctcttaaaa ctataactta
     1561 caggccgata tccattagca ctcacctttt cttcactgcg cttaatttcc atgacatgag
     1621 cacgatcata agtcttatcg gcaaattctt tagttgtttc gtcatggtta gcagtaccaa
     1681 taaaccagac attttgagga actttaatcg cacgttggtc actaaaatag cgtggcgact
     1741 tttcgttagg gctttctagt agtgcaattt ccgaaaggtc aggggtttcc atgagagaga
     1801 ggaattcagc aaaatattgt tctggctgtg ataaattcat ttcatcaagt aagataattt
     1861 gaactgtatc tgaaaactgg ggttgctgag cgcgataaat cgcctgcaat gcttccctct
     1921 cgtagaattt tttctcgaag gcattaaaat ggcctagcaa atcctctcgg tcacgccatc
     1981 cagcctgtac gcgaaccatt gagcaatata gcttttcttg atcgccagta gtgacgttat
     2041 taatcgcttt cgcgaacgca cgcgccaaac tagttttacc tgtgccagac atgccctgta
     2101 gtaaatgcag tttgctcatc gcaagtcctg caataaacaa acgaatgtcc tttaggctat
     2161 aaaataactc ttgttgagca atccctacct gcattgcctt agcaaattgc gctagatctt
     2221 gtggaacagc ttgcaaacca cgtgcaggat gctgaaattg ctgatccatt ttactaagcg
     2281 cagggaatgg tagttctcct tgctggcgtg ctgagagatc atcaatttgc agtttaagct
     2341 gttcaatact aatatccagc aatcgattat gctcatgtaa tacgcgtttt tctcgtaata
     2401 gattatattt ttcctgtgca ctcattcgaa gagtgtctcg ctgggcattt gcttcggaca
     2461 attggaattc tgtatcttgg agtttttctt caatataaga taacttttta cagagttttt
     2521 gattgtcttc tagtaggcca tcttcttctc gctgtgcaag atcttctttt aatcgacgat
     2581 tttcaagacg aagacgctcc atctcggctt gtaacgatgt cacatttcgt tcggctaact
     2641 ctcgttttaa atcttcaagc tgagacaact cttcagtaag ctgatttatt ttttcaaaag
     2701 cttgtttttt gttattttca gtgtgtatta gtttcgtctg caactcttgt aatttgagct
     2761 ggtagttttg ctctatctcc tcttctttag ctgcctccca tactgtttgc tcttgtcgtt
     2821 gttttttcaa acgatgttct aatatagaaa gacttgcttg cttttgttga gttaattggt
     2881 cgagatgttg ttgtttatct aaaaaacctg cttctgcctg tgattcacgt tcaattaata
     2941 aacgctcgcg ctctagtaat tcttttttct tttgctccaa cgagtcgctt gatgacttta
     3001 agtcactcaa gtgctgcttg tgcaattgct caagctcttg gcgtttttcc ttaatggcta
     3061 tttgtttttc caaagcaaaa tccatctcag cagcttgttt aagcttttcc aattccgctt
     3121 tctgctcttc caattggaga agagaaactt caagctcacg ctgcttttgc tcaatctcag
     3181 attttagttt atccacttca ctctgttgtc gttgtacggc tgattgttga cgtgatagtg
     3241 aaacttgttc gcgaagtaat gccgtctgtt gtttttctaa gtcgattttt tgtgtgctaa
     3301 actcatgaga cttcgtatcg taagt
//
LOCUS       DAOQLJ010000224         3289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_15715_length_3289_cov_5.753865, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000224 DAOQLJ010000000
VERSION     DAOQLJ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15715_length_3289_cov_5.753865"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PK522_11250"
     CDS             <1..1018
                     /locus_tag="PK522_11250"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNC42350.1"
                     /translation="QSALMTADSLVTGDYVLQETDITIPALHVTAYEGGVTQTRSWGG
                     RDNGFCQQGGYDVLERAGFIGSGQLAAQEALQLLAAPNCPSGKFDLLLMPDQLMLQIH
                     ESIGHPLELDRILGDERNYAGTSFVTLDMFGSYQYGSPLLNVTYDPTQATELATFAYD
                     DEGMQAQRQYIIQNGILLQPLGGMQSNRRAQQMGYAFDAVATSRACAWNRPPIDRMSN
                     LNVEPGTATLDEMIASIEDGLLMKTNASWSIDDSRNKFQFGCEWGQRIRNGKLAEIVR
                     NPNYRGISKTFWRSLAMVGNASTFEVMGTPYCGKGEPNQAIWVGHASPACKFTGIDVF
                     GAER"
     gene            1015..2337
                     /locus_tag="PK522_11255"
     CDS             1015..2337
                     /locus_tag="PK522_11255"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNC42351.1"
                     /translation="MKAYFFELVDFALGQLTGTEVLLLKFDGEASDFVRFNHGRVRQP
                     LSVTQRYLALTLIQDQRQQRVTFSLTGNKATDCELLTQQLGVMRHQLPNLPIDPYLLY
                     ATDGCDSDQVRRGVLPTIDQAIGDIVDQAGSLDLVGILSSGPIYRGFANSLGQRNWHE
                     VDAFLFDWSIYQVADKAIKMSYSGTHWDQSQLRSRIESARTQLPYLEKTAVNIEPGEY
                     PAYLSAAAVNELLGLLNWSGFSAKEQHTQQSVLQALVNKERTLSPHITLRENTQLGLA
                     PAFDSVGFVKSDQITLIDAGQHASSLISPRTAKEYGLQANGANESETTQSLELLPGTL
                     DSTNLLEQLDEGIYVSNLHYLNFSDRTACRMTGLTRFATFWVEQGKIVAPLNVMRFDD
                     SLYRMLGSHLMGLTRETEWLLSSDTYGQRSVGSSRVPGALLSKLRLTL"
     gene            complement(2759..>3289)
                     /locus_tag="PK522_11260"
     CDS             complement(2759..>3289)
                     /locus_tag="PK522_11260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC42352.1"
                     /translation="HDELTGLANRYLLYDRIQQLLSDIERNHGSAAMLMIDLNDFKKI
                     NDTLGHDIGDLLLKEVSKRLTSTLRAIDTVARLGGDEFVVVTPHVSVAGIKLVVQKII
                     HLLPYPIFIANNEMKIGVSIGITLIPSEGKDTKTLLKNCDIAMYRAKKMGRNNYQFFS
                     GELSNEVTVLNPKTLD"
BASE COUNT          885 a          709 c          769 g          926 t
ORIGIN      
        1 ccaaagcgca ctgatgaccg cggatagcct ggtgactggt gattatgtat tgcaggaaac
       61 cgatatcact attccagcgc tgcatgttac tgcttatgag ggcggagtca cccaaacgcg
      121 atcttggggt gggcgggata atggcttttg tcagcaaggg ggttatgatg tgctagagcg
      181 cgctgggttt attggcagtg ggcagcttgc ggcgcaggaa gcattgcaat tactggcagc
      241 gccgaattgc ccgagtggaa aattcgattt gttactgatg cccgatcaac tcatgttgca
      301 aatccatgaa tcgattggcc atccgctcga actggatcgt atcttagggg atgagcgcaa
      361 ctatgctggt actagctttg taacgcttga tatgtttggt tcctaccagt atgggtcacc
      421 attgttgaat gttacttacg atcctactca agcaactgaa ctggctactt ttgcgtatga
      481 cgatgaaggc atgcaagccc aacgccaata tatcattcag aacggcatct tgttgcaacc
      541 cctcggtggc atgcaatcta accggcgcgc ccagcaaatg ggttatgcat ttgatgccgt
      601 agcgacatcc cgtgcctgcg cttggaatcg accaccgatc gatcgcatga gtaatttgaa
      661 tgtcgaaccg ggtactgcaa cgctcgatga aatgattgct tcaatcgaag atggcctact
      721 gatgaaaacc aacgcaagtt ggtcgatcga tgactcacgc aataaatttc aatttggttg
      781 tgagtggggg caacgcattc gcaacggtaa attggctgaa atcgttcgta accctaatta
      841 tcgcggtatt tctaaaacct tctggcgttc attagccatg gtaggaaacg catcgacctt
      901 cgaagtcatg ggaacgcctt attgcggcaa aggtgaaccg aatcaggcga tttgggttgg
      961 ccatgccagc ccggcgtgta aattcactgg gattgatgta tttggagcag agcgatgaaa
     1021 gcgtatttct ttgaactcgt tgattttgca ttgggccagc ttactggaac agaggtgttg
     1081 ttgctcaaat tcgatggcga agcttctgat tttgtgcgct ttaatcacgg tcgtgtgcgc
     1141 cagcctttat cagtaacgca acgttatctt gcgctaacgt tgattcaaga ccagcgtcaa
     1201 caacgcgtca ccttttcttt gaccggtaat aaagccaccg attgcgagct gctgactcag
     1261 caattggggg tgatgcgtca tcaattgcca aatttgccga tcgacccgta tttgctttat
     1321 gcaaccgatg gctgtgattc cgatcaggtg cgccgcggtg tattgccaac catcgatcaa
     1381 gcaattggcg atatcgtgga tcaagcaggc agtctcgatt tagtgggtat tttgtccagt
     1441 ggaccgatct atcgcggttt tgctaactca ctagggcaac gtaattggca cgaggtcgat
     1501 gctttcttgt tcgattggtc tatttatcag gttgcggata aagcgatcaa aatgagctac
     1561 tcaggaacgc attgggatca atcgcagcta aggagccgaa ttgaatcagc gcgtacgcag
     1621 ctaccgtatc tcgagaaaac tgcagttaac attgaaccag gggaatatcc agcctatctg
     1681 agcgctgctg ccgtcaatga actgttaggg ttgctgaatt ggagcggttt ttccgccaag
     1741 gagcaacata cgcagcaaag cgttttgcag gccttggtga ataaagagcg aacgctttcc
     1801 ccgcacatca cgctacgaga aaatactcag cttggcttgg cacctgcttt tgatagtgtt
     1861 ggctttgtaa aatcagacca gattacgttg atcgatgccg gacagcatgc gagtagttta
     1921 atatcgccac gcaccgccaa agaatatgga ttacaagcca atggcgccaa tgaaagcgaa
     1981 acgacgcagt cgctcgaact gctaccaggc acgcttgata gcaccaacct acttgaacag
     2041 ctcgatgaag gcatctatgt cagcaatctg cattatctca atttctcaga tcgtaccgct
     2101 tgccgcatga cggggctaac gcgttttgca actttctggg ttgaacaagg aaaaattgta
     2161 gcgccgctca atgtcatgcg tttcgatgac agcctttatc ggatgttagg tagtcatctc
     2221 atgggattga cacgagaaac ggaatggttg ttaagctcag atacttatgg tcagcgatca
     2281 gtaggctcca gccgtgttcc tggtgcgtta ttatcgaagc tgcggttgac gctgtgaata
     2341 catagatctg taacagtcga taatcaaaaa atatctagga gtagaattcc tcgtcacgaa
     2401 gtacgaggaa tatacactga catgcttatt tacgaaaagt acatcgatat cagaattaaa
     2461 gatgctgcca ccaaccctat aatggctata ttaatgccca atttagtcat cgtcatagaa
     2521 tcttctttct ctagcatttc catcattacc tcctaaaatc aatgattaac ggaaattgaa
     2581 ttttccacct aatcaatagg ttagtcgttg atcaagatca ataaagtcaa tcaaattttt
     2641 tgatttagat caaaaaatga aaactaggcg ggggtgctca tctaaggagc aaaactaatt
     2701 taaagtgatt tttatgatta aactggtatg aaagtactag catgatagat gtactcgact
     2761 aatctaaagt tttaggattg agtacagtaa cttcgttgct gagttcccct gaaaagaact
     2821 gataattgtt acgtcccatc ttttttgcac gatacattgc aatatcacaa ttttttaata
     2881 gtgttttggt atcctttcct tctgatggga ttagcgttat accaatgctt acaccaattt
     2941 tcatttcatt attagctata aatattggat acggtagaag atgtatgatt ttctgtacaa
     3001 ctaattttat tcctgcgact gaaacatgtg gcgttactac tacaaactca tcaccaccta
     3061 agcgcgcaac tgtatctatt gcgcgcaaag tagatgttaa tcttttactg acttctttga
     3121 gcaagaggtc accaatatca tgtcctaatg tatcgttgat ttttttgaaa tcattcagat
     3181 cgatcatcaa catggcagcc gatccgtggt ttcgttcgat atcagatagc aactgctgta
     3241 tacgatcgta gagtaaatat cgattagcga gccctgttaa ctcatcatg
//
LOCUS       DAOQLJ010000225         3200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_16480_length_3200_cov_4.375199, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000225 DAOQLJ010000000
VERSION     DAOQLJ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16480_length_3200_cov_4.375199"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..1208
                     /locus_tag="PK522_11265"
     CDS             45..1208
                     /locus_tag="PK522_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC42353.1"
                     /translation="MKIATIVQNQQCLNDIQKYLAQAGSSHPLVGVTGGAQQISAVIE
                     QEHPDLLLIEGLRLNPGEQQILSKVTTCFPDIGVIMLCPPQPQEFLLEAMRVGVREVV
                     PTPIAQNVLIDAVERFQQRMSQAKMPVRQGKVLAFMPCKGGSGATFLASNIAYALAAL
                     ENKRVILIDFNLQFGDASLFVHDGAAKTTVADVARQIQRLDGSFLSSSLIQVLPNFGV
                     LAAPDEPEKAAEIKLEHIKPLLQVAIKHYDFVVLDIGRSLDAISIQCLDQADYIFPVL
                     QQTLPFIRDAKRLINTFFSLGYPAEKIRLIVNRYDKKDEITLADVANTLKLEVFKAIP
                     NDYNVVAESVNQGIPVTKLAKRSAVAKSLHEFVQEMAQSSTSNKLLKKLFAFG"
     gene            1264..2637
                     /locus_tag="PK522_11270"
     CDS             1264..2637
                     /locus_tag="PK522_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNC42354.1"
                     /translation="MSIRDRLQGIGSNLTATASQVAVTNPNHFTSYDNDAYHELKSRI
                     HQALLNRMDLAVMESLPQDRLLVEIKGLVERLLIEERTPINDIERLRIIQDIQHEVLG
                     LGPLEPLLADPTISDILVNTHRQVYVERRGKLELTDTQFANEAHLRKIIDRIVSRVGR
                     RVDESSPMVDARLPDGSRVNAIIPPLAIDGALLSIRRFAVIPLKIEDLIRNKSMTQEM
                     SEIISGLVKAKCSILISGGTGSGKTTLLNIMSAYIPHSERIVTIEDAAELQLQQPHVV
                     RLETRPPNVEGKGEVSQRALVRNSLRMRPDRVIIGEVRGAEAIDMLQAMNTGHEGSMA
                     TVHANTPRDALSRIENMVSMAGLNLPTKAVRHQISSAIVVVIQVSRLSDGKRKLINIQ
                     EITGMEGDVITMQEIYTFQQTGVAADGTVQGHFRATGIRPKFAERLKIYGTALREDLF
                     DPTRRYE"
     gene            2647..>3200
                     /locus_tag="PK522_11275"
     CDS             2647..>3200
                     /locus_tag="PK522_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadB"
                     /protein_id="HNC42355.1"
                     /translation="MDYLYYLYVALAFLAVVLFLEGIYFIWRSYQGAEAKRIQKRLQE
                     IAAGWNDTASASLIKQRLLSDSPALQKWLLKLPRVHAVDRLLLQSGMAIMVMPFLGYS
                     LLTGLGGMALAAILGFSFAVILLSMVVGCLIPYFFVLRARHKRLITIEQQLPEIIDLM
                     SRALKAGHAFTGALQMAATEGTEPA"
BASE COUNT          888 a          642 c          787 g          883 t
ORIGIN      
        1 tatttgaaca aattatctga attaatgggt agaggagtaa tacattgaaa atcgctacaa
       61 ttgtacaaaa ccaacagtgt ctaaatgata ttcagaagta tttggcacag gcagggtcat
      121 cgcatccact ggttggggtt acaggggggg cgcaacaaat ctctgctgta attgaacaag
      181 aacaccctga tctattattg atcgaagggc tacggttgaa tcctggggaa cagcaaatct
      241 tgagtaaagt aacgacgtgt ttcccagata tcggggtgat tatgttatgt ccgccgcaac
      301 cacaagagtt tttacttgaa gcgatgcgag ttggggtgcg cgaagtggta cccacgccga
      361 ttgctcagaa tgttctgatc gatgcggtcg aacgtttcca gcaacggatg tcccaagcta
      421 aaatgcccgt tcggcaagga aaagtactgg ctttcatgcc gtgtaagggt ggcagcgggg
      481 caacatttct ggcaagtaat atcgcctatg cgctcgctgc gcttgaaaac aagcgagtaa
      541 tccttatcga cttcaatctg cagtttggtg atgcttcgct gtttgtgcac gatggtgcag
      601 ccaaaaccac tgttgcagat gttgcaagac aaattcaacg actagatgga tcatttctgt
      661 cttcgagttt aattcaggta ttaccaaatt ttggcgtgct agctgcaccg gatgagcccg
      721 agaaagctgc ggaaatcaag cttgagcata tcaagccttt attacaagtg gcaatcaagc
      781 attatgattt tgttgttctc gatatcgggc gttccttaga tgcgatttcg attcaatgct
      841 tagatcaggc cgattatatt tttccagtac tccagcaaac attgccattt attcgcgatg
      901 ccaagcgttt gatcaatact tttttctctt tgggttaccc tgctgaaaaa atacgcttaa
      961 ttgtcaatcg atacgacaag aaagatgaga ttacactggc tgacgtggca aataccttaa
     1021 agctagaagt ctttaaagcc ataccgaatg actataacgt tgttgctgag tctgtcaatc
     1081 aaggaatacc ggtgactaaa ttagcgaaac gaagtgcagt agcgaagtcg ctgcatgaat
     1141 ttgtccaaga aatggctcag agttcaacca gcaataagtt gctgaaaaaa ttatttgcgt
     1201 ttggttagca tcattgctgc tgtggatgat aggaagaaaa tttaacttaa tggaacattg
     1261 attatgtcaa ttcgagatcg tcttcaaggg ataggatcga acctaacagc gactgcaagc
     1321 caggttgcgg tcactaatcc taatcatttt acttcttatg ataatgatgc ttaccatgaa
     1381 ttgaagtctc ggatacatca agcattgctc aatcgcatgg atttggcagt gatggaaagt
     1441 cttccgcaag atcgattgtt ggtggaaatt aaggggttgg tggaacgact cctcatcgag
     1501 gaacgaacac caatcaatga tatcgagaga ttacgcatta ttcaggatat ccagcatgaa
     1561 gtgctgggat tgggtcccct tgagccctta ctggctgacc cgacgatttc agatatttta
     1621 gtcaataccc atcgtcaagt ttatgttgaa cgacgaggaa aactggaact gaccgatacc
     1681 caatttgcca acgaggcaca tttaagaaaa attatcgatc ggatcgtttc tagagttggg
     1741 cgtagagtcg atgaatcgag tccgatggtt gatgcccgat tgccggatgg ttctcgtgtc
     1801 aatgcaatca taccacccct tgcgattgat ggcgctttgt tatcgattcg gcgctttgca
     1861 gtgattccgc tcaagataga agatttgatt cgcaataaat caatgaccca agaaatgagc
     1921 gagattatta gcggtttggt taaggcaaag tgcagcatac tcatttcggg gggtaccggt
     1981 agcggtaaaa caacgctgct gaatatcatg tctgcgtata ttccgcattc tgagcgaatt
     2041 gttaccattg aagacgcagc cgaattacag ttacagcagc cccatgtcgt gcggttggaa
     2101 acaagacctc ccaatgttga gggtaaagga gaagtttcgc aacgtgcttt ggttcgaaac
     2161 agcttacgga tgcggcctga tcgcgtcatt attggtgagg tacgcggtgc tgaggcgatc
     2221 gatatgttgc aagccatgaa taccggtcac gaaggttcga tggcaacggt tcatgcgaat
     2281 accccccgag atgcgttgag tcgaattgaa aatatggtca gtatggctgg ccttaactta
     2341 cctaccaaag cggttcggca ccaaattagt tcagctattg tcgtggtgat acaagtttcg
     2401 cgattgtcgg atggaaaacg taagttaatc aatattcaag aaataactgg aatggaaggc
     2461 gatgtcatta ccatgcagga aatttatacc ttccagcaaa cgggggtagc agcggatgga
     2521 acagtacagg gtcattttag agcgacggga attagaccca aatttgcgga acgcctcaaa
     2581 atatatggta cagcactccg cgaggattta tttgatccca ctcgccgcta tgaataaggg
     2641 gaagtaatgg attatctcta ttatctttat gttgcgctcg cttttctcgc tgtagtgctt
     2701 tttctcgagg gaatttactt tatctggcga tcgtatcaag gtgcagaagc caaacggatt
     2761 caaaaacggc tacaggagat tgctgcggga tggaacgata ctgcaagtgc atcattgatc
     2821 aagcaacgat tattgagtga ttcgcccgca ttacaaaaat ggttacttaa attgcctcgg
     2881 gtccatgcgg tagatcgatt gctgctccaa tccggtatgg caatcatggt catgccattt
     2941 ttaggatata gcctgttgac cggattggga ggaatggctt tagccgctat attaggtttt
     3001 tcatttgctg tcattttgtt aagtatggtg gtcggctgct taatccctta tttttttgtg
     3061 ctaagagcta ggcataaacg gttgattacc atagagcagc aattacccga aataatcgat
     3121 ttgatgtctc gcgcgctcaa agcaggtcat gcgtttactg gcgcattgca aatggctgca
     3181 accgaaggaa cggaacctgc
//
LOCUS       DAOQLJ010000226         3146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_16964_length_3146_cov_5.466839, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000226 DAOQLJ010000000
VERSION     DAOQLJ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16964_length_3146_cov_5.466839"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1052)
                     /locus_tag="PK522_11280"
     CDS             complement(132..1052)
                     /locus_tag="PK522_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNC42356.1"
                     /translation="MAHNHSPDHDHSHVPKNFHRAFAIGIALNAGFVVVEIVFGLFAN
                     SLALLADAGHNLSDVLSLLFAWGASVLVRKLPTQRHTYGLRRTSILAALINALVLLVA
                     VGAIAWEAILRFGHPEAVASDTVIWVASVGIVINGFTAWLFMSGQKQDMNIRGAYLHM
                     MADAAVSFGVVLAAITIKFTGWLWLDPVMSLVVAVIIGIGTWGLLRESLSLALDAVPA
                     NIHPSKVEAYLASLPGIEAVHDLHIWAMSTTEIALTVHLVKPDAVIDDLLLVQINNEL
                     RELFGIQHTTVQFERGDTSHPCKQAPAEVV"
     gene            1250..2248
                     /locus_tag="PK522_11285"
     CDS             1250..2248
                     /locus_tag="PK522_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance protein"
                     /protein_id="HNC42357.1"
                     /translation="MRREQLEENQIGLYAVALAVGMGLGLWNSSFGAGLEYLISPVLA
                     VLLYSMFAQIPFLQLREAFANKRFTAALLLVNFSIVPIVVWLLSFSLPEHSPLLLGVY
                     LVLLTPCIDYVVVFTHLGRGNARLVLASTPLLLLTQMLLLPVYLWLFMGEQAAQVMSA
                     EPFLEAFLVLIVFPLGLALLTEFWAKRQRSGAIWLEVTAWFPVPFMAVTLLLVVASQI
                     GRIEEYLPVVEQVVPVYIAFMAIMPFLSRLIAYAFRLDTQAGRALVFSAGTRNSLVVL
                     PLALALPDGWILASVVIVTQTLVELIGELIYIRLVPSIIFQESKSLEISKVLSKKK"
     gene            complement(2319..2933)
                     /locus_tag="PK522_11290"
     CDS             complement(2319..2933)
                     /locus_tag="PK522_11290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC42358.1"
                     /translation="MDNKQHWEQVYTTKASDSVSWFQEHADQSLRLIHNTRLGKDAAI
                     IDVGGGTSRLVDDLVAEGYTDLTVFDLSSTALAVARQRLGKHANIVHWIEGDITRAKF
                     PMNRFDIWHDRAVFHFLTDPADRHAYVERVLSSVRPGGHVIVATFAEDGPEKCSGLPV
                     MRYQPEALHAVFGDTFQLVEHEKEAHHTPFGTVQQFVYCYCRKR"
     gene            complement(2946..>3146)
                     /locus_tag="PK522_11295"
     CDS             complement(2946..>3146)
                     /locus_tag="PK522_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HNC42359.1"
                     /translation="VEVESLDRDKFDITGQIHSFSDIGGAILAFGTCLKLRESEELGI
                     CSISTMQDLYTLIHDSDKVLCF"
BASE COUNT          848 a          720 c          697 g          881 t
ORIGIN      
        1 cgcttcagta cttggctcta gcgtttcagt agctaattta accggacact actgattaag
       61 tttacaaaac ctgcggagct ctcaaataat tatttattat tatgataaaa caaaagttac
      121 tcatttctac tttaaaccac ttctgccggt gcttgcttac agggatggga tgtatctccg
      181 cgctcaaatt ggacggtggt atgctgaatt ccaaacagct cacgcaattc attattgatt
      241 tgaaccaata gcaaatcatc aatgaccgca tctggcttga ctagatgcac ggttagtgca
      301 atttccgtcg tgctcattgc ccagatatgt aggtcgtgaa ctgcttcaat ccctggaaga
      361 ctagcgaggt aagcttcaac tttggacgga tggatatttg ctggaacagc atcaagagca
      421 aggcttaacg attcacggag caagccccaa gtgccaatac caataattac ggcgaccaca
      481 aggctcatga ctgggtccaa ccacaaccag cccgtaaatt ttatggtaat agctgccaaa
      541 acaacgccaa aagaaactgc tgcgtcagcc atcatgtgca aatatgcacc gcgaatattc
      601 atatcttgct tttggccgga catgaacagc catgccgtga acccgttgat gactatgcca
      661 actgatgcca cccagatgac tgtgtcactt gccactgcct cgggatggcc aaatctgaga
      721 atggcttccc aggcgatagc gccaacagca acaagcaaaa ctagcgcatt aatcaatgca
      781 gccaaaatag aagtacggcg caaaccgtaa gtatgccgct gtgttggtaa cttgcgcact
      841 aagacgcttg cgccccacgc gaataataaa ctcaaaacat cgctgaggtt atggcctgca
      901 tctgccaaca gcgcgagaga atttgcaaat aaaccaaaaa caatttcaac cacaacgaaa
      961 cctgcattta gcgcaatccc tattgcgaat gcgcgatgaa agtttttggg aacatggcta
     1021 tgatcatgat cgggggagtg gttatgtgcc atatatagat tctctttccg acatgtgaat
     1081 ttctaaaaat agcggatata ttaaaattag atggaaccga tcggagattc gtagactgaa
     1141 ctgcaatggt tatgtcaaca tgatcagaaa cattgaaata aaacgctgcg ccaaattatg
     1201 gcacaaattg ctcactatcc cattacggta tgtattttaa ggaaacacaa tgcgaagaga
     1261 acaactggaa gaaaatcaga tagggttata tgctgtggcc ttggctgtcg ggatggggct
     1321 tgggttgtgg aattctagct ttggcgcagg cctggagtat ttgatttccc ccgtgcttgc
     1381 agtattgctt tatagcatgt ttgcgcaaat cccttttctt caactacgcg aggcgttcgc
     1441 gaataagcgg tttacagctg cgctattgct agttaatttt agcatcgtgc ccatcgttgt
     1501 ttggttattg tcattttctt tgccggagca ctcgccgctg ttactagggg tatatctggt
     1561 tctgttaaca ccttgtattg attacgttgt cgtttttact caccttggtc gtggcaatgc
     1621 ccggcttgta cttgcttcca cgcctttgtt gctgcttacc cagatgcttc ttttgcccgt
     1681 gtatctatgg cttttcatgg gagaacaagc tgcacaagtc atgagtgctg agcctttcct
     1741 tgaggctttt cttgtattga ttgtgtttcc tctagggctt gccttactaa cggagttttg
     1801 ggcaaagcgc cagcgtagcg gtgcaatctg gcttgaagtc acggcgtggt ttccggtgcc
     1861 atttatggcg gttacattac ttctcgtcgt ggcatctcaa attggcagaa tagaagaata
     1921 cctccctgtt gtggaacaag ttgtgccagt ttatattgcc tttatggcta tcatgccctt
     1981 tctttctcgc ttgatagcat atgcatttcg cctggacaca caggcaggaa gagcacttgt
     2041 ttttagcgca gggacgcgga attcgttggt ggttctgcct ttagcgctgg ctttgccgga
     2101 tggttggatt ttggcatctg ttgtaatcgt gactcaaacg ctggtggaac taatcggtga
     2161 gctgatttac atacgattgg tgccttcgat tatctttcaa gagtctaaaa gtttagaaat
     2221 ctcaaaagtg cttagcaaaa agaagtaatt aggataaaca agttgtgctt ctatccggga
     2281 aagacattca gttttaaaaa tattcggaga attagctatt atctcttgcg gcagtagcaa
     2341 taaacaaact gctgaacagt gccaaatggc gtgtggtgtg cttctttttc atgttcgacc
     2401 agctgaaaag tatctccaaa taccgcatgt agggcttcag gttggtatct catcacgggt
     2461 agaccactac atttctctgg cccatcttcg gcaaaagtgg caacaatcac atggccgcct
     2521 ggccgaaccg aacttaagac acgctcgaca taagcatggc gatctgccgg atcagtcaaa
     2581 aaatggaaca ctgcacggtc atgccaaatg tcgaatcggt tcatgggaaa ctttgcacgg
     2641 gtaatatcgc cttctatcca gtgaacaata ttcgcatgtt tccccaaacg ctgccttgca
     2701 acagcaagcg cagtcgatga cagatcaaat actgtcaaat cagtatagcc ttctgccaca
     2761 aggtcatcta ctagtctgga agtcccccct cccacatcaa taatggccgc atctttaccg
     2821 agtcttgtat tatggattaa tcgcagagac tggtcagcgt gttcctgaaa ccaactaact
     2881 gagtcagatg ccttagtggt atatacctgt tcccaatgct gcttattatc catggggtaa
     2941 tttctctaaa agcacaatac cttatctgaa tcgtgaatca gcgtatataa atcctgcatc
     3001 gtggatatcg aacagattcc taactcttca gactcacgaa gcttaagaca ggttccgaaa
     3061 gctaaaattg caccgcctat atccgaaaaa ctgtgaatct ggccggtaat atcaaacttg
     3121 tccctatcaa gagattctac ctccac
//
LOCUS       DAOQLJ010000227         3138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_17044_length_3138_cov_4.086604, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000227 DAOQLJ010000000
VERSION     DAOQLJ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17044_length_3138_cov_4.086604"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PK522_11300"
     CDS             complement(<1..705)
                     /locus_tag="PK522_11300"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNC42360.1"
                     /translation="MIESNEKLSQTRQCQLLSLARSTFYYQPQPLDNEKLALLRKLDE
                     QYLKTPQYGARSYATWFVRQGISIGRKRTANMMKILGIVSTAPKPKTSNSDKRHKTYP
                     YLLKGVVINRANQVWTADITYVPMEKGFGYLVAIMDWHSRKVLSWRLSNTLDADFCIQ
                     ALEAAIQDYGCPEIFNTDQGAQFTAEAWICILKNHGIQISMDGKGRYHDNIFIERLWR
                     TVKYELLYTRAFSNLKE"
     gene            complement(726..1007)
                     /locus_tag="PK522_11305"
     CDS             complement(726..1007)
                     /locus_tag="PK522_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC42361.1"
                     /translation="MSKKRKQYSSEFKAKVALAAIRGEETVPQLAARHGLHPTQINSW
                     KRQLIEQAAEVFSKDQSAGKADTSTKDDLHRVIGQLTVERDFLARKLNH"
     gene            complement(1090..1581)
                     /locus_tag="PK522_11310"
     CDS             complement(1090..1581)
                     /locus_tag="PK522_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42362.1"
                     /translation="MKRITIIVSASDMPAIRKVVFIAGANKLVIAKVPHRTCIGELDD
                     WYCGTHIAQREDHMRLEVTSDDTRSDGVISAILSSAHAAKIENISSGVNRAGSKDSYL
                     HHCPICETAHQVNHIRHIMAHGRSITCSVTCEIQRRKKWKQHGNFAKKKVFVGLKRES
                     MSI"
     gene            2221..>3138
                     /locus_tag="PK522_11315"
     CDS             2221..>3138
                     /locus_tag="PK522_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNC42363.1"
                     /translation="MKSIVLVACLLTSASAFPQDCNKIIINAPSSPLTFGAYAETYYS
                     YDFNNLINNTKPPFLFSFNKNNEASVNLAFLKGSYVTDRVRANLGLAAGTYMNANYAA
                     EPGILGNLFEGNAGIKLSGGNNLWLDVGVFPSHIGFESAVGKDNWTLTRSLVAENTPY
                     FESGAKISYTSDNDKWFLSALILNGWQHIKPVDGNTIPAFGTQITYKPSPRVTLNSST
                     FLGSDKPDSARQMRYFHNFYAIFKLNDTLSTTIGFDIGVEQRTKDSSNMNTWFNPTMI
                     LRYTPTIKTAVAIRAEYYDDKHGVIIASDT"
BASE COUNT          850 a          708 c          639 g          941 t
ORIGIN      
        1 ctccttcaag ttactgaatg ctctggtgta taagagctca tacttgactg tacgccatag
       61 ccgttcgata aagatattat catgatagcg gcctttgcca tccatgctga tctgaatgcc
      121 gtggtttttc aggatacaaa tccatgcttc tgcggtaaat tgagcgcctt gatcggtgtt
      181 gaatatctcc gggcaaccgt agtcctggat agcagcttcc agagcttgga tacagaaatc
      241 ggcatccagt gtgttggata aacgccagct cagcactttg cgagagtgcc aatccatgat
      301 ggcaacgaga taaccgaaac ctttctccat cggcacatac gtgatatctg ccgtccacac
      361 ttgattggct cggttgataa caacgccctt gagcaggtac ggataggtct tgtgccgctt
      421 gtcagaattg ctggttttgg gcttgggtgc tgtgctgaca atccccagaa tcttcatcat
      481 gtttgcagtc ctcttgcgcc cgatactgat accttgacgc acaaaccagg tagcatagct
      541 gcgtgcgccg tattggggcg ttttcaggta ttgctcatcc agtttccgca ataaagccag
      601 tttctcatta tcgaggggct gtggctgata ataaaaggtg gagcgcgcta aactcagcag
      661 ctgacactgg cgagtttggc tcagcttctc attagactca atcatcttct tgcgctgtac
      721 aagacttaat gattgagctt tctggccaaa aaatcgcgtt ctaccgtcaa ttgcccgatg
      781 acgcgatgca aatcatcctt agtgcttgta tctgcctttc cagcgctttg gtctttggaa
      841 aatacttctg ctgcttgttc aattaattgt cgcttccagc tattgatttg cgttggatgc
      901 aaaccgtgac gcgctgccaa ctggggtacc gtttcctctc cacgtatcgc agccaacgcc
      961 acttttgcct tgaattcact tgaatattgc ttgcgtttct tactcatctc attgctcctt
     1021 ctagcacaat gtcagagctt aactgcttgt acagtttttg ggggccactt caactctcaa
     1081 caactggcac taaatcgaca tcgattcacg cttcagacct acgaaaactt ttttcttagc
     1141 aaagtttcca tgctgtttcc atttttttct tctctgtatc tcacaagtga cactgcaggt
     1201 tatggatctt ccgtgagcca tgatgtggcg gatatgattg acttggtgcg ctgtttcaca
     1261 tattgggcaa tggtgtaaat aagaatcctt gctgcctgct ctgttcacgc ccgaagagat
     1321 attctcgatc tttgctgcat gcgcggagga gagaatagcg gaaattactc catcagatcg
     1381 agtatcatcg gacgtaactt ccaatcgcat atgatcttca cgctgggcga tgtgagtccc
     1441 acagtaccag tcgtcgagtt caccgataca tgttcgatgt ggcaccttgg caattaccaa
     1501 tttattcgca cctgcgataa aaaccacttt acgtattgcc ggcatatccg aagcgctcac
     1561 aatgattgtg atgcgtttca tgctattact ccttctctaa caatttcaca aaacaaaaac
     1621 aaatgttaga agaaacagcg taaaaacgtt gtaaattact ggctaccgcc tgtaaagaat
     1681 tcgtatataa atattcgatc ctgagaaatg tctcagactc tcatgaatac agtgttttag
     1741 gcaataatcc agccttttaa actcctgaag atttataata tcggcattga aacctgagga
     1801 aaatcagagg aattccagat ggtgactaac gattcttttg tccctggctc gatcaaatat
     1861 gataaaaaag agccttctat tttcggattc catttctatt taccgttgat cttggaaaaa
     1921 tcagttcaat gattcttttt actccgctat ttatatagcg gcactgcttc cttcgacata
     1981 gccgctgcta acatttagac ctcagaaatt tttaggtatc aaaagctgcg accattcctt
     2041 caattgatgt gtctatccaa cgcaattggc agtgcataaa atcagagaca aattttacgg
     2101 attttttata gttacacgtt atctatttac gatattttta tatcttatct tatatattca
     2161 gttacttgaa taactaatta gcatccaaag gaatattcga tggaagctta gaagatagag
     2221 atgaaaagta ttgtgttggt tgcttgcctg ctgacaagcg caagtgcttt cccacaagat
     2281 tgcaacaaaa ttataattaa tgccccctct tctccattaa cctttggagc ctatgccgaa
     2341 acttattata gctacgattt caataatctg atcaataaca ccaaaccccc gtttctcttc
     2401 agcttcaaca aaaataatga agcttcggta aatttagctt ttttaaaagg atcgtacgtt
     2461 acagacagag tcagggctaa tcttggactg gcagcgggta cctacatgaa cgccaattat
     2521 gcggctgagc ccggcatatt ggggaattta ttcgaaggca atgccggaat aaagttatct
     2581 ggaggaaata acctgtggct ggatgtcggc gtcttccctt cacatatcgg ctttgaaagt
     2641 gctgtgggaa aagacaattg gactttaacc cgaagcttgg tagctgagaa cacaccatat
     2701 tttgaatccg gtgccaaaat cagttacacc tctgacaatg acaaatggtt tttaagtgca
     2761 ttaattctga atggttggca gcacatcaag ccagtggatg gaaatactat tcctgcattc
     2821 gggacccaga ttacttataa accttctcca agagtaacct tgaatagcag cacttttctc
     2881 ggcagtgaca agcctgacag tgcgaggcaa atgcgctatt ttcataattt ctacgctatt
     2941 tttaaattga atgatacgct ctctaccact atcggttttg atattggcgt cgagcaaagg
     3001 accaaagact cctcaaatat gaatacttgg ttcaatccta caatgatttt gagatataca
     3061 cccacaataa aaactgctgt tgcaatcagg gcggaatatt acgatgacaa acacggtgtg
     3121 atcatcgctt cagatact
//
LOCUS       DAOQLJ010000228         3122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_17207_length_3122_cov_6.661559, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000228 DAOQLJ010000000
VERSION     DAOQLJ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17207_length_3122_cov_6.661559"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PK522_11320"
     CDS             complement(<1..194)
                     /locus_tag="PK522_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010893244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HNC42364.1"
                     /translation="MHNPAHPGEILREWLPEEMTVTQAAKELQISRVTLSKVLNGKAG
                     ITATMALRLSAWLGTTPDVWL"
     gene            complement(221..502)
                     /locus_tag="PK522_11325"
     CDS             complement(221..502)
                     /locus_tag="PK522_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HNC42365.1"
                     /translation="MQIKTKSGRVLVLPSPEEDAQITAAALTDPDNQPLTNEELQQFK
                     KVRGRPQGSGKKEQVTLRLDTDILEQFKASGNGWQTRINDALRDWIKQH"
     gene            complement(486..755)
                     /locus_tag="PK522_11330"
     CDS             complement(486..755)
                     /locus_tag="PK522_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNC42366.1"
                     /translation="MKVICDATKDAINKEKHGVSLHDAMHIDWDAALIWQDTRHDYGE
                     VRMIALAPIDERLYCVVYVDREDHRRIISLRKANYREVIHYANQD"
     gene            complement(809..2098)
                     /locus_tag="PK522_11335"
     CDS             complement(809..2098)
                     /locus_tag="PK522_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HNC42367.1"
                     /translation="MKRKGFTAKVISEFQCEFGKSQSLFWDADHPGLGLRVAKGGKKS
                     YIFETKLHGRTLRLTIGDVLSWSLNRAEKEATRLKALTDQGIDPRQLEAEKRVAAEVK
                     RKREHAEKINLRDAWEEYINERKSLWSDHHLNDHIKVMHEGGQIRGRSKELTVPGVLS
                     SLAKVRLVDLTHERIEEWAKIESIKRPTRARLALRLLKAFLFWCSRHSSYKEIVIDNA
                     AQSKTARENLGKAKAKNDALQREQLAAWFQAARQIPNPVISAYLQSLLLTGSRREELA
                     NLRWEDCDFQWNSLTIRDKVEGQRVIPLTPYVSQLLSNLPRRNHWVFSSPTAATGRLI
                     EPNKQHSKICSIIGVSVSLHGLRRSFATLSEWVETPAGIAAQIQGHKPSGVREKNYIR
                     RPLDLLRLWHTKIETWILEQAGIEFIPIKAGLHVVNK"
     gene            complement(2388..>3122)
                     /locus_tag="PK522_11340"
     CDS             complement(2388..>3122)
                     /locus_tag="PK522_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC42368.1"
                     /translation="AMARLPRFVLVGHPQHIIIRGNNREPVFNAEEDYRFFLDKLADA
                     AKKHQCDIHAYVLMTNHVHLLATPHKAESIAKMMQMLGRYYVQYYNYTYQRTGTLWEG
                     RYKASLIDSEQYALLCYRYIELNPLRADMVKHPSEYPWSSYRSNALGQYDALITPHSM
                     YIALDQDEAQRQARYRDLFNGQIDEQNLASIREATNKAWVLGSDYFKQKIEAQLNRRA
                     DKLTKGGDRKSDEFRQNKKFNRIRPH"
BASE COUNT          761 a          696 c          612 g         1053 t
ORIGIN      
        1 agccaaacat ctggtgttgt acccagccaa gcagaaagcc taagtgccat tgtcgctgtt
       61 attccagctt taccgtttag taccttcgaa agtgtcacac gtgaaatctg taattctttt
      121 gcggcttgtg ttacggtcat ttcttcaggt aaccattctc gtaatatctc gcctggatgt
      181 gctggattgt gcattttact cacaggtatt tttcctctta tcaatgctgc tttatccagt
      241 cccttaacgc atcattgatg cgtgtttgcc agccattgcc gctcgccttg aattgctcca
      301 atatatctgt atctagtcga agcgttacct gttccttttt cccgcttcct tgaggtcttc
      361 cgcgcacttt tttgaattgc tgcaattctt cattcgttaa aggttgatta tcaggatcag
      421 taagtgctgc tgctgtaatc tgtgcatctt cttctggtga aggtagaact aagacgcgac
      481 ctgatttagt cttgatttgc ataatgaatt acctctctat aatttgcttt acgtaagctg
      541 attattcgtc gatgatcttc acgatcaaca taaaccacgc aatacaacct ttcatcaatt
      601 ggcgctaagg cgatcattcg aacttcccca taatcgtgcc gcgtgtcttg ccatatcaac
      661 gcagcatccc agtcaatgtg cattgcatcg tgcagtgata cgccatgctt ttccttattt
      721 attgcatctt tagtggcatc gcaaatcact ttcatatatt aatgtagtta cgtttattgc
      781 aagcgtcaag gattatttgt agctacgttt atttatttac aacgtgcaat cctgctttta
      841 ttggtataaa ttcaatccca gcttgctcta aaatccacgt ttctattttg gtgtgccata
      901 gccttagtaa atcgagtggg cgcctaatgt aattcttctc acgtactccg cttggtttgt
      961 ggccttggat ttgggcagct attcccgctg gtgtttctac ccattccgat aaggtagcga
     1021 atgaacgcct taatccatgc aatgaaacac tcacaccaat gattgagcat atcttgctat
     1081 gttgtttatt cggttctata aggcggccag ttgcagcagt ggggctactg aatacccagt
     1141 gatttctacg gggcaaatta ctcaataact gtgatacgta aggggttaac gggatcactc
     1201 tttggccttc taccttgtcg cgtatggtca aactattcca ttgaaagtcg caatcttccc
     1261 aacgtagatt ggctaattct tcacgacgtg atccggttag taataaggat tgcagataag
     1321 cactaataac tgggtttgga atttgcctgg ctgcttgaaa ccaagcagct aattgttcgc
     1381 gttgcaatgc gtcatttttt gctttggctt tacctagatt ctcccgtgca gttttacttt
     1441 gtgctgcgtt atcaataaca atttctttat agcttgaatg cctgctacac caaaacaaaa
     1501 aggcttttaa tagtcttaat gcaagcctag ctctagtagg tcttttgatc gattcgattt
     1561 ttgcccattc ttcgatacgt tcatgagtta aatcaacaag gcgaactttg gctaaagaag
     1621 aaagtacacc cggaacagtt aattctttac tgcgaccgcg tatttgcccc ccttcatgca
     1681 ttactttgat gtgatcattt aagtggtgat ccgaccatag tgacttacgc tcattgatgt
     1741 attcttccca cgcatcacga agatttattt tttcggcgtg ttctcttttt ctttttactt
     1801 cagcagctac tcttttctcc gcttctagct ggcgaggatc aattccttga tcagtcagcg
     1861 cttttagcct tgttgcttcc ttttctgctc tattaagcga ccatgagagt acatcgccaa
     1921 tagttaagcg taacgttctg ccatgtaatt ttgtttcaaa gatataagac ttttttcctc
     1981 ctttggctac acgtaaacct aatcctggat gatcagcatc ccaaaaaaga ctttgcgact
     2041 tgccaaattc acattgaaat tcagaaatca ctttagctgt aaaacctttt cttttcattc
     2101 cgtgtagtct ccgtgtagga aatatgtagg aaattttgat caattatgct aaatttaaat
     2161 caacacggat tttaacacga aatgacataa cttattgata ttagttgatt tagcaatcat
     2221 tttctaaacc taaatcaacg tcaataaatg actagttgcg aggactgaaa atccttgtgt
     2281 cggtggttcg attccgccct tggccaccaa taaccatgcg gttattagcg atttttgatt
     2341 agattatttt ttccgcaaaa gctgtgcata actcgaatca aagcgaatca atggggtcgg
     2401 attcgattga atttcttgtt ttgtctaaat tcatctgatt ttctatctcc accctttgtc
     2461 agtttatccg ctctgcggtt aagttgcgct tctatctttt gcttgaaata atcacttcct
     2521 aacacccaag ccttgtttgt agcttctctg atgcttgcca agttttgctc atctatttgc
     2581 ccgttaaaca aatcacggta tcgcgcttgt ctttgtgcct cgtcttgatc taatgcaata
     2641 tacatcgaat gcggcgtaat taacgcatca tattgcccca aagcattgct gcgataactg
     2701 ctccacgggt attccgatgg gtgcttaacc atatcagcac gtaatggatt aagctctatg
     2761 tagcgatagc acaacagggc gtattgttcg ctgtctatca agctggcttt atagcgtcct
     2821 tcccataagg tgccggtacg ttgatacgta tagttgtagt attgcacata atatcgtccc
     2881 agcatttgca tcatcttggc gatgctttcg gctttgtgtg gggttgctag caggtgaaca
     2941 tggttggtca ttagcacata ggcgtggatg tcacattgat gctttttggc tgcatcagcc
     3001 aacttatcca gaaagaaacg gtagtcttct tctgcattaa acactggctc gcggttgttg
     3061 ccacgaataa tgatgtgctg gggatgtcca accaaaacaa aacggggaag gcgcgccatg
     3121 gc
//
LOCUS       DAOQLJ010000229         3097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_17456_length_3097_cov_5.950362, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000229 DAOQLJ010000000
VERSION     DAOQLJ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17456_length_3097_cov_5.950362"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..1421
                     /locus_tag="PK522_11345"
     CDS             6..1421
                     /locus_tag="PK522_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNC42369.1"
                     /translation="MKILYHRQSINLLLRWVFSLFFLLMVTACALGPDYRRPAIEVAE
                     HYRIPHAEGESIANLPWWELFQDKVLQQLIQQALQENKDLKRVISSIEEYQARLSMAN
                     MDFAPQLDTDITAPVMGKIGGFSRPGFATPFNYFGMVNLNWEIDIWGRIRRSTQAAFA
                     ELIAQEANRRAIVLSLVGAVAQSYFDLLQLDKQLELAQQILHSWEESVAISDAQLQHG
                     LISKLDNDQFQAERANAAARVAILEREMVQKENELSLLLGKSPNSVLRGSAINEQWLS
                     PEIPAGLPSELLDRRPDILRDEQVLAAATARIGATKAARLPRISLTGFLGVASPSLSN
                     LLLSNSQFGVGGLGLAAPILNARALGFEQKAVEAQAKQALAQYEQTALIAFKEVEDAL
                     VAIRTVKDQYKATQEQVKALQSALQIADLRYQSGLTSYVDVLLAKRSLFDAETALVST
                     QRFHLVALVQLYKALGGGWAP"
     gene            1542..2765
                     /locus_tag="PK522_11350"
     CDS             1542..2765
                     /locus_tag="PK522_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC42370.1"
                     /translation="MMYPLISWVFRGARQIKLQAIYVGLGLFVCLVIGCSDKPAADLA
                     ASPLPQVAVITLKTQSVADEPEFIGQTQSFRPVEIRSQVSGIIKQIFFTEGRNIKKGD
                     RLYQIDPIPFRAAYQRGQANVAQAKVRLKQAQQDLARVRPLLEKKAVSKKNIDDAEAE
                     VQAAKATLDAAQNELTKAKFDLDNTLIYSPVNGRIGRSQFYEGRLIEAQESLLTAIAQ
                     LDPMYVNVNVPESYLLKRRRELVEQKVASPELFQLRGEMTFSDGTVYPEEGVLDFADI
                     RLRPETGTLQGRFRFSNPDGKGEPSVRNFLSGQFVKVRVKGYIRHNAILVPQRAVHQG
                     PKGSFVFIINEEDKVELRSVLASSWRGKEWLIESGLSAGERVVVEGFFRILPGVKVNA
                     VAYSLIENESASRHD"
     gene            2790..>3097
                     /locus_tag="PK522_11355"
     CDS             2790..>3097
                     /locus_tag="PK522_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC42371.1"
                     /translation="MNSHFFIDRPIFASVLSIIIVVIGLVSLQTLPVAQFPQITPPMV
                     QIDADYPGASAEVVAEAVARPIEVQLPGIDNLLYYESTSTNDGHMMMRLTFEIGTDID
                     "
BASE COUNT          857 a          601 c          762 g          877 t
ORIGIN      
        1 ttaagatgaa aattctttac catcgacagt caatcaattt actgcttcgc tgggtgttct
       61 cactgttttt tttgctaatg gttactgcat gtgcattagg gcctgattat cgtcgtcctg
      121 caatcgaagt ggccgagcat tatcgaatac ctcatgcgga aggagaatcg attgcaaacc
      181 ttccttggtg ggagctattt caagataaag tgcttcagca attgattcaa caagcattac
      241 aagaaaataa agacctaaaa cgtgtaattt caagtattga ggagtaccag gcgcgcttat
      301 cgatggcaaa tatggatttt gcgccgcaac tagatacgga tatcactgct ccagttatgg
      361 gcaaaatagg aggattttct cgtcctggat tcgctacacc cttcaactat ttcgggatgg
      421 ttaatttaaa ttgggaaatc gatatctggg gaaggattcg tcgttcgacc caagctgcgt
      481 ttgcggaatt gattgcccaa gaagcgaatc gtcgtgctat cgtgctatcg ttagtgggag
      541 cagttgcgca aagttatttc gatttgttgc aactagataa gcaacttgaa cttgctcagc
      601 aaatattgca ttcttgggag gaatccgttg ctatttccga tgctcaattg caacacggct
      661 taatttcgaa gttagataac gaccaatttc aagccgagcg cgcgaatgcg gctgccagag
      721 tagcgatatt agagcgtgaa atggtacaga aagaaaatga actgagtctt ttgctaggca
      781 agagtccaaa ttcagtacta cgtggttccg caatcaatga acaatggctt tctcctgaaa
      841 tccctgctgg cttgccgtct gaattactag atcgccggcc tgatattttg cgggatgagc
      901 aagtactagc ggcagctact gctagaattg gggcaacgaa agctgcgcgc cttcctcgaa
      961 tttctttaac aggttttttg ggggttgcaa gcccttcatt gtcgaatcta ttgttatcga
     1021 atagtcagtt tggagtaggg gggttggggc tcgcagcgcc aatattgaat gcacgcgcat
     1081 tgggcttcga acaaaaagct gtcgaagcac aagcaaagca ggcgcttgca caatatgagc
     1141 aaactgcctt gattgctttt aaagaggtgg aagatgcact ggtcgcgatt cggaccgtca
     1201 aagaccaata taaggcaact caagaacaag ttaaggcatt acagtcagcg ctacaaattg
     1261 cggatcttcg ctatcaaagt ggactaacaa gctatgttga tgtgctgctt gctaaacgta
     1321 gcttatttga tgcagaaaca gcgttggttt caactcaacg gttccattta gtggcacttg
     1381 ttcagcttta taaagcctta gggggtgggt gggcgccttg aagatacacg ctgttgtgct
     1441 gtgcaggtac ccaaattcaa tctatgcggc tggtatttag gccgatattt gattatcgta
     1501 tcaatcttat tgttcaatcg aggttttcta ctaacgaaat catgatgtat ccattaattt
     1561 catgggtttt tcggggtgct cgtcaaatta aactgcaggc aatttatgtc ggtttggggt
     1621 tattcgtttg cctcgtgatc ggttgttcag ataagcctgc cgcagatctt gctgcttcac
     1681 cattacccca agtagcagtg ataacgctaa agactcagtc agtagctgat gagcctgagt
     1741 ttattgggca aacacaatca tttcgaccgg tagaaattcg atcgcaagtg agtgggatta
     1801 tcaaacagat tttttttacg gaaggtagaa atattaaaaa aggagatcga ctttatcaga
     1861 ttgacccgat tccttttaga gcagcttacc aacgaggtca agccaatgtt gcgcaagcga
     1921 aagttagatt aaagcaggct cagcaagatt tagctagagt gcgtcctttg ttggagaaga
     1981 aagcagttag caaaaaaaat attgatgatg cagaagcaga ggttcaagca gcaaaggcga
     2041 cattggatgc agcacagaat gagttgacta aggcaaaatt tgacctggat aacacgctga
     2101 tttattcacc ggtgaatggg agaatcggtc gcagtcagtt ttatgaaggg cgccttatcg
     2161 aggctcaaga atccttatta acagccattg ctcaactgga tcccatgtat gtgaatgtca
     2221 atgttcccga aagctattta ttgaaacggc ggcgtgaact ggttgagcaa aaagtggcaa
     2281 gccccgaact ttttcagctg cgtggggaaa tgactttttc agatggcact gtttacccgg
     2341 aagaaggagt acttgatttt gcggatataa gactacgccc agaaacaggt acactgcaag
     2401 ggcgtttccg attctcgaat cccgatggaa aaggggagcc aagtgtcagg aattttttat
     2461 cgggccaatt tgtcaaggtt cgagtcaaag gttatattcg ccacaatgcc atacttgttc
     2521 cgcagcgcgc tgttcatcaa gggcccaaag gatcattcgt ttttataatt aatgaagaag
     2581 ataaagtgga acttcgttca gttttagcaa gcagctggcg tgggaaagaa tggctcatcg
     2641 agagtggatt aagtgcggga gaaagagtcg tggtagaagg tttttttcgt attttacctg
     2701 gagtcaaggt gaatgcagtt gcttattctc tgatagagaa cgaatctgct tcacggcatg
     2761 attaacgtaa tttgtactaa ataactttca tgaattctca tttttttatc gatcgaccta
     2821 tttttgcgtc ggtactatcg attatcatcg tagtaatcgg gttagtttct ttgcaaacat
     2881 tacccgttgc acagtttccg cagataacgc ccccgatggt acaaatcgat gcggattatc
     2941 caggagcgag tgcagaggtg gttgcagagg ctgttgctcg accaattgag gtgcaattgc
     3001 ctggtattga taatttattg tattacgaat cgactagcac gaatgatggg cacatgatga
     3061 tgcgcctcac attcgaaatt ggaaccgata tcgatat
//
LOCUS       DAOQLJ010000230         3056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_17879_length_3056_cov_6.689770, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000230 DAOQLJ010000000
VERSION     DAOQLJ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17879_length_3056_cov_6.689770"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1791)
                     /gene="ftsH"
                     /locus_tag="PK522_11360"
     CDS             complement(<1..1791)
                     /gene="ftsH"
                     /locus_tag="PK522_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNC42372.1"
                     /translation="MDNNEVNKKLQFNFWYIVIAILGMLFLQNLYTQYTRIEPIPYSQ
                     FQDLLEREKIAEVAITENQIYGTLKASEPNGMKEFVATRVEPELAEILEKHNVIYTGV
                     IRSTWLRDLLSWIVPMLLFVGIWLFIMRRVSGGMSSGLMSIGKSRAKVYVETETKVTF
                     ADVAGVDEAKEELIEIVNFLKNPADYSRLGGRAPKGILLVGPPGTGKTLLARAVAGEA
                     GVPFFSISGSEFVEMFVGVGAARVRDLFEQARQMAPAIIFIDELDSLGRARGAYGLGG
                     HDEKEQTLNQLLAELDGFDSSSGIVLLAATNRPEILDAALLRAGRFDRQVLIDRPDKT
                     GREQILAVHLKKVKLGVDVMPDQIAALTPGFTGADLANLINEAALLATRRKATSVSMD
                     DFNNAIERIIAGLEKRNRLLNLNERKIVAFHELGHAMVALALPGTDEIHKVSIIPRGV
                     GALGYTIQRPTEDRFLMTKPELENKMAVLLGGRAAEQVIFNQVSTGASDDLVRATDIA
                     RAMVLRYGMSEALGHVAYEREHSAFLQQGYALPQSREYSEATANKIDEAVRLLVDQAL
                     ERAISILRKNQTLLEQTAIDLLKAETLTQPN"
     gene            2296..3042
                     /locus_tag="PK522_11365"
     CDS             2296..3042
                     /locus_tag="PK522_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42373.1"
                     /translation="MSKIVLSGPVLYVHDNAGKLATLDATTGSIDIIGDMGITMTDIA
                     FSPTGDLYGISFGRLYAIDSDTAQATLIGSHAVPGGNTLVFGEDGTLYSAGYMSTSLY
                     TIDVSNGATTNLGSMGFFAGGDLAFHNGHLYMASLSNKLVDIDLDDLSNTVAIGNFAI
                     GGVFGLATGSDNNLYAVANTAVFSVNTTTGGVIDLVSFAKQGLGQAFGLTSFIPDDIV
                     IAAEIAEPTTIMLLLSGLLGFRRFSKSGQK"
BASE COUNT          842 a          649 c          635 g          930 t
ORIGIN      
        1 attaggctga gtcagtgttt cagctttcaa taggtcgatc gcggtttgct ccagcagcgt
       61 ctgattctta cgtaaaatac tgatagctcg ttccaatgcc tgatctacta gcaaacgaac
      121 agcttcgtcg attttattag cggttgcttc gctatattct ctactctggg gcagcgcata
      181 accttgttgt aaaaacgcag aatgttctcg ttcataagca acatggccta gcgcttcgct
      241 catgccatag cgtaacacca tggcacgcgc aatatcggtt gcacgaacta aatcatccga
      301 ggcaccggtt gagacctgat tgaaaataac ttgctcagca gcacgtcctc cgagtaacac
      361 agccattttg ttttctaatt cgggctttgt catgagaaag cggtcttcgg tcgggcgttg
      421 gatggtataa ccgagcgcgc caacgccacg tggaataatg gatactttat gaatctcatc
      481 cgttccaggc agagcaagcg cgaccatagc gtgccctagt tcgtgaaatg cgacgatctt
      541 acgttcatta agattcaaca agcgattgcg cttttcgagg ccggcaatga tgcgctcgat
      601 ggcattgttg aaatcgtcca tgctaacgga agtagctttg cggcgagttg ccaataatgc
      661 agcttcattg attaaattag ccaaatcagc gccagtaaag cctggtgtta aggctgcaat
      721 ttgatcgggc atgacatcca caccgagctt tactttcttg aggtgtacag ccaagatttg
      781 ctctcttccc gttttatcgg gccgatcaat taatacttgt cggtcaaatc gccctgctct
      841 cagtaatgca gcatcaagaa tttcgggccg attggtggct gctaacaata cgataccact
      901 cgatgaatca aaaccatcta attcagccag taattggtta agcgtttgct ctttttcatc
      961 gtgcccaccg agcccatagg caccgcgtgc cctgcccaat gaatccagtt catcgataaa
     1021 aataatagct ggcgccattt gtcgtgcttg ctcaaataaa tcgcgtactc gtgcagcacc
     1081 cacgccgacg aacatctcga caaattctga accggagatt gaaaaaaaag gaacgcccgc
     1141 ttctcccgcg acggctcgtg caagtaatgt tttcccagta ccaggggggc caaccaatag
     1201 aatgccttta ggtgcgcgtc caccaagtcg actataatca gcagggtttt ttaaaaaatt
     1261 gacaatctcg atcaattctt ctttcgcttc atcaacacca gcgacatccg caaaagttac
     1321 tttggtttca gtctccacat aaactttcgc ccgacttttt cctatcgaca ttaaaccact
     1381 actcatcccg ccactaacgc ggcgcatgat aaaaagccag ataccaacaa acagtaacat
     1441 aggcacaatc catgagagta agtcgcgtag ccaggtactt ctgataacgc ccgtgtaaat
     1501 tacattatgt ttttccagaa tttctgctag ttcaggttcg actcgagtgg caacaaactc
     1561 tttcatccca ttgggttccg atgcttttaa tgtgccataa atctgatttt cagtaatagc
     1621 cacctctgca attttttctc gctctagtaa atcttggaat tggctataag gaatgggctc
     1681 aatgcgcgtg tattgtgtat aaagattttg tagaaataac ataccgagga tggcaatgac
     1741 gatataccaa aaattgaact ggagtttctt atttacttca ttgttatcca tatgaatatt
     1801 ccttctttcg attgatttag tccaaatttg atcagggcta cttgaattcg tacggtaatt
     1861 gtaaattcaa tcattccaca ccaaaaaaat gtattttttt actttacggt taataactaa
     1921 agattttaca tatgggatag atcagatatc tcctttttat tcaatgaatt ggaattgtgt
     1981 gttgctttac catgactcct cttgcgtcga ttaacttaac aaattattta gattatttta
     2041 tcttgatgcg taagcgacag taaaaaattg tttaaataca taatcttata tctattattt
     2101 gattttggca tgagatctgc ttagagattg atacgtactt atttctagtt ttcacaaata
     2161 gtaaagatta gatagcaaga aatagatcaa cgttttaact aaaagctttg ttaagcatta
     2221 aggattgata tgaaatatag cgcaagaact cccgtaacaa ttatcagttt cttattttta
     2281 ctgatgctta ctggtatgag caagattgta ctcagtggcc cagtattata cgtacacgat
     2341 aacgctggta aattggcgac gcttgatgca acaacgggat ctatcgatat tatcggggat
     2401 atgggcatca caatgacgga tatcgcgttt agtccgacag gtgatttgta tggaatttcc
     2461 tttggacgtt tatacgctat cgattcagat acggcgcaag ccactctgat tggcagtcat
     2521 gcagttccag gcggaaatac ccttgtattc ggtgaggatg ggacgctcta ttctgctggt
     2581 tatatgagta catcacttta tacaatcgat gtaagtaatg gtgcaacgac taatcttggc
     2641 agcatgggtt tttttgcagg aggcgattta gcttttcata acggccacct ttacatggca
     2701 tctttgagca ataaactcgt tgatatcgac ttagacgatt tatcgaatac ggttgcgatt
     2761 ggtaattttg ctattggtgg agtttttggt ttagcgactg ggagtgataa caatttgtat
     2821 gccgttgcta acacagcagt tttttctgtt aacaccacta cagggggtgt tattgatcta
     2881 gtgagctttg cgaagcaggg attaggacaa gcttttgggt taacaagttt tattcctgac
     2941 gacattgtta ttgcagctga aattgctgaa cccacaacga ttatgctgct cttatccgga
     3001 ttacttggtt ttagaagatt tagtaaatcc ggtcaaaaat gaaccaagat ttccat
//
LOCUS       DAOQLJ010000231         2986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_18669_length_2986_cov_6.187649, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000231 DAOQLJ010000000
VERSION     DAOQLJ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18669_length_2986_cov_6.187649"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /gene="hslU"
                     /locus_tag="PK522_11370"
     CDS             complement(<1..1210)
                     /gene="hslU"
                     /locus_tag="PK522_11370"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNC42374.1"
                     /translation="MSQMTPQEIVHELDKHIIGQINAKRAVAIALRNRWRRQQIADPL
                     RQEITPKNILMIGPTGVGKTEIARRLAKLADAPFIKVEATKFTEVGYVGRDVDSIIRD
                     LVESAIKQGREQEIRKMRPLAEDRAEERILDVLLPPARDLDFRAENEDSNATRQKFRK
                     KLREGELDNKEIEIEIAGAQASMEIFAPPGMEELTSQIQSMFQNMGSSKKKARKLMIR
                     DAKKILIEEEAARLINDEELKLRCVQNVEQNGIVFLDEIDKITSRSETAGADVSRQGV
                     QRDLLPLVEGTTISTKYGMIKTDHILFIASGAFHLAKPSDLIPELQGRFPIRVELTSL
                     TAADFEQILTNTDACLTRQYEALLKTEGVILNFAESAIKRLAEIAFSVNEKTENIGAR
                     RLHTVIEKLLE"
     gene            complement(1242..1763)
                     /gene="hslV"
                     /locus_tag="PK522_11375"
     CDS             complement(1242..1763)
                     /gene="hslV"
                     /locus_tag="PK522_11375"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNC42375.1"
                     /translation="MTTIVSVRRGKQVALGGDGQVTLGAVVAKSSARKVRRLYHGKIL
                     AGFAGGTADAFTLFERFEGKLEKHQGHLMRSAVELAKDWRTDRMLRRLEAMLVVADHE
                     ATLIITGAGDVIEPELSLAAIGSGGAYAQSAARALLENTELSAKDIVSKALTIAGDLC
                     IYTNQAHIIESLD"
     gene            1906..2976
                     /locus_tag="PK522_11380"
     CDS             1906..2976
                     /locus_tag="PK522_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HNC42376.1"
                     /translation="MPNLMSELHLATMTLSNRIIMAPLTRCRASTDHVPNALMAEYYA
                     QRASAGLIIAEATMIMAGHSAFWKEPGIYSEAQINSWQQITTAVHRAGGRIFLQLWHG
                     GRACHPLLNGGAQPISASAIPILGDEVHTPEGKKPYVTPREIADSEIPSIITGFKTAA
                     ENAKAADFDGVEIHGANGYLLDQFLRDGANKRHGPYGGSIEKRARLMLEVIEAVSQVW
                     ESHKVGLRISPLNSYNDMIDSDPISLTRWLASHLNDYNLAYLHLIRGDILQKQKGDII
                     TPARTHYKGILIGNQGYTPQEAEYAVKEGIVDAIAFGVPFIANPDLPLRIKNKAPLNQ
                     ANPARFYTSGPEGYTDYPFWQP"
BASE COUNT          803 a          730 c          603 g          850 t
ORIGIN      
        1 cttcaagtaa tttctctatc acggtatgta gccgccttgc accgatattc tcggtttttt
       61 cattcactga aaaagcaatt tcagctaatc gtttgattgc actttccgca aaattcagta
      121 tgactccttc tgttttaagc agcgcttcat actgacgcgt caaacacgca tccgtattgg
      181 taagaatttg ctcaaaatca gccgctgtca ggcttgtcag ctcaacccgt atcggaaaac
      241 gtccttgcaa ctcaggaata aggtcggaag gtttagccaa gtgaaatgca ccgcttgcaa
      301 taaacaagat atgatcagtc ttaatcatgc catatttggt tgatatcgtc gtgccttcaa
      361 ccaaaggtaa taagtcacgt tgtactcctt ggcgtgaaac atccgccccc gccgtctcgg
      421 aacgactcgt aattttgtcg atttcatcca agaatacaat tccattttgt tccacgttct
      481 gcacgcacct taacttcaat tcttcatcat tgatgagtcg agctgcttct tcttcaatga
      541 gaattttctt cgcatcacgt atcatcaact tacgtgcttt ctttttgcta gaacccatgt
      601 tctggaacat actttgaatt tgtgaagtca actcctccat cccaggtggt gcaaaaatct
      661 ccatgcttgc ctgtgcacca gcgatttcaa tttcaatctc tttattatcg agctcacctt
      721 cgcgtaattt tttacgaaat ttctggcgcg ttgcgttgga atcctcattc tcagcacgaa
      781 aatccaggtc acgagcgggc ggcagtaaaa catcaagaat tcgttcttct gcgcgatctt
      841 cggcgagtgg tctcattttt cgtatttctt gctcacgccc ctgcttaatt gcggactcaa
      901 ccaaatcacg aataatcgaa tcaacatcac gccccacgta accgacttca gtaaatttag
      961 tcgcttcgac tttaataaaa ggtgcatcag ctaatttagc cagacggcgc gcaatttcag
     1021 ttttgcctac cccggtaggc ccaatcatga gaatattttt aggcgtgatc tcttgacgta
     1081 atggatcagc aatctgctgg cgacgccagc gattgcgcag tgcaattgca actgcacgct
     1141 tggcattgat ttgtccaata atatgtttat ccaattcgtg gacaatttct tgtggggtca
     1201 tttgtgacat aaatttattg cttattaaaa aattcaatca attaatcgag agactcgatg
     1261 atatgcgctt gatttgtgta aatacacaaa tccccagcga tggtcagcgc tttcgagacg
     1321 atatctttag cagaaagctc tgtgttttca agcaaagcac gcgctgctga ttgcgcatag
     1381 gcgccaccac tcccaattgc agcaagacta agctccggct caatcacatc ccctgcgccg
     1441 gtaataatca aggttgcttc atgatcagcc accaccagca tggcctcaag acgccgcagc
     1501 atgcgatccg ttcgccaatc tttggctagt tccaccgctg atcgcatcaa gtgaccttgg
     1561 tgcttctcca atttaccttc aaaccgctca aatagcgtga acgcatccgc cgttcctccg
     1621 gcaaacccag ccaaaatttt tccatggtaa agccgtctta ctttgcgcgc gctcgattta
     1681 gcaaccacgg caccgagcgt aacctgccca tcccctccta gagcaacttg cttaccgcgc
     1741 ctcactgata cgatggtagt catgttattt attattagat taaagtacta attatagcgc
     1801 tgattctaag gggttatgaa attgtaaaaa aagcgtgcgg agataattta gaccttgctt
     1861 tagaatttct gctaggcttg aaactttatc aattctatct taatcatgcc taatttaatg
     1921 agtgagctgc atctagcaac aatgacactc tctaatcgaa ttatcatggc accgctcaca
     1981 cgctgtcgag ctagtactga ccacgtacct aatgccttga tggcagaata ttatgcacaa
     2041 cgcgcaagcg ctggcctgat catcgctgaa gcaactatga tcatggcggg ccattctgct
     2101 ttctggaaag aacccggtat ttattctgaa gcacaaatta acagttggca gcagataacc
     2161 acagccgttc atcgtgcagg ggggcgaatc tttttacaac tttggcacgg tggccgtgca
     2221 tgccatccgc tgcttaatgg gggcgcgcaa cctattagcg cgagcgccat tcccattcta
     2281 ggtgatgaag ttcacacgcc tgaaggaaag aaaccatacg ttactccacg tgaaatcgct
     2341 gatagtgaaa taccttccat tatcaccgga tttaaaactg ctgctgaaaa tgcaaaagct
     2401 gctgatttcg atggtgttga aattcatggt gccaatggat atctactgga tcaattttta
     2461 cgcgatggcg caaacaaacg ccatggtcct tatggtggct caatagaaaa acgagccaga
     2521 ctcatgctgg aagtcattga agcggtcagt caggtctggg aaagccacaa agtggggtta
     2581 cgcatctcac cgcttaacag ctacaacgat atgattgata gcgatccaat aagtttgact
     2641 cggtggttag caagtcattt gaatgattat aatcttgctt atttacattt gattcgaggc
     2701 gatatcttgc agaaacaaaa aggggatatc attacacctg cacgcactca ttataagggt
     2761 atcttaatcg gtaaccaagg ctatactcca caagaagctg agtatgccgt caaagaaggc
     2821 atcgtcgatg caatcgcttt tggagtcccc tttatcgcta atcctgattt gccgttgcga
     2881 attaaaaaca aagcaccgct caatcaagca aatccagcta ggttttatac atctggacct
     2941 gaaggttata ccgattatcc tttttggcaa ccttaatttt agttta
//
LOCUS       DAOQLJ010000232         2976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_18794_length_2976_cov_5.084560, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000232 DAOQLJ010000000
VERSION     DAOQLJ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18794_length_2976_cov_5.084560"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..1697)
                     /locus_tag="PK522_11385"
     CDS             complement(297..1697)
                     /locus_tag="PK522_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNC42377.1"
                     /translation="MSMLLGNVSLQASEKITTSQDQKSITVTIYNENLALIKDSRNVE
                     LSQNINQLAWREVSALIRPETALLGNQTSQSGFHVFEQNFDFDLLTPQKLLEKHVGKE
                     ITVVRTNLATGTETNETAVVLSTNEGIVLKFNNRIETGAPGRMVFPGVPENLRDKPTL
                     LLLLHSPESGKHELELSYLTGGLSWRADYVAVLSENDSKLDLNGLVTLTNQSGVAYPQ
                     ARLQLVAGDVNQIQSEQRIGRKMAAMSVEMADAAQMKEESLFEYHLYTLQRPTTLAEN
                     QTKQVALMSASQIPVNKIFFLQGADYYYSGKYGVLGQKLKVSVLISFDNKGEQLGIPL
                     PKGIVRVYKKDVQGYSQFIGEDQIDHTPKNEQIKLKLGSAFDITADKIQTDFQQIAGS
                     IHQTSIFETTYQITIRNAKKESVVVQVQEPIPGDWVIVSESIKHKKISANTVEWKIPV
                     SAEGEATFVYRVRVKY"
     gene            complement(1747..>2976)
                     /locus_tag="PK522_11390"
     CDS             complement(1747..>2976)
                     /locus_tag="PK522_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42378.1"
                     /translation="KTAAELRAEADAKAAEAAIARAEADKAAAANAEATAKAQLEAEA
                     IAISRAQAEARLLEEINQKITAEKEAQFSAESRAQAEQQEVATLSAKMNADNAAVEAT
                     KERIKAQAEAIKKSRERAIAEATAKQAAEERMAAEAKAAAEVEDRAKVDKLAAEAAIE
                     LAEIEARLAADARKRHDAIVQAKISITSRIENEQRMTELASKIAQARIVAAVKTEEKL
                     KATEIAASTTFARVQAETSALNAIQARIESDALAIEQAIAREAVEAMAKEAADARTKA
                     DQRAIAQASQQIQQDLAETEIIRNNQSAEETTEEQATFSQEDYVGSPLIEQYEEEESS
                     ELSDENEYDEEFRADIQDLADSPIEDSTELVEYVEINEIDFGWQTELDSESTTNDNDS
                     SASDEELEEDNRGKIFS"
BASE COUNT          756 a          689 c          522 g         1009 t
ORIGIN      
        1 tgtgtaaagc atgccgccag cgttcaatct gagccaggat caaactcttc agtttaatca
       61 ctcatatatt tacaatatta attacttata ttgctatcaa gcgcctacaa tttattggtt
      121 attagatttt aaagaactta tcgctttcaa aattacttac tgcgcgattg aagatgtgca
      181 ttatacaagc attttcattt tggtcaagct ttattgtaaa aaatgtagag aatcgatgga
      241 tcagtaaaaa taatatggct ttagactaat ggatttagtc taaagccata ttattactaa
      301 tatttaaccc gcactcgata aacaaacgtc gcttcaccct ccgctgaaac tggaatcttc
      361 cactcaacag tgttagcaga tattttttta tgttttatag attcagaaac aattacccaa
      421 tcacctggaa ttggttcctg aacctgcaca acgactgact ctttcttagc atttctaatc
      481 gtaatttgat atgtcgtttc aaaaatactt gtttgatgta tcgaacctgc aatctgttga
      541 aaatcagttt gtatcttatc agcagttata tcaaatgcac ttcccagttt taatttaatc
      601 tgttcgtttt tcggagtatg gtcaatttga tcttcgccaa taaactgact ataaccttgc
      661 acatctttct tataaacacg aacgatcccc tttggcaacg gaataccgag ctgttcacct
      721 ttattatcaa agctaattaa tacacttact ttgagctttt ggccaagcac gccatatttt
      781 ccggagtaat agtaatctgc accttgaagg aaaaatattt tattaactgg aatttgacta
      841 gcagacatga gagccacttg cttagtttga ttttctgcca gcgttgttgg ccgctgaagt
      901 gtataaagat ggtactcaaa aagtgattct tctttcatct gggcagcatc agccatttct
      961 accgacatag ccgccatttt tctaccaatg cgttgttcag attgaatttg attaacatcc
     1021 ccagcaacca attgcaatct agcctgagga tacgccaccc cgctttgatt ggttagcgtc
     1081 actaatccat ttaaatcgag cttactatca ttttcactga ggaccgcaac ataatcggca
     1141 cgccaagaaa gaccacccgt caagtacgag agctcaagtt catgcttacc cgattctggg
     1201 ctatgcagca atagtaataa agtaggttta tcacgcaaat tttctggcac acctggaaat
     1261 accattcgtc caggcgcacc cgtttcaata cgattattaa atttgagaac aattccctca
     1321 ttggttgaga gaacgaccgc cgtttcattt gtttctgtgc cagtagccaa attagtccgg
     1381 accacagtaa tttcttttcc tacatgtttc tctaacaatt tttgtggcgt cagcaggtca
     1441 aaatcaaaat tttgctcaaa aacatgaaaa ccagattgag atgtttgatt accgagtaaa
     1501 gcggtttcag gacgaattag tgcggaaact tcacgccagg ccagctgatt aatgttttga
     1561 cttaactcaa cattacgaga atccttaatt aaggccagat tttcgttata aatcgtaact
     1621 gtaatacttt tttgatcctg gctcgttgtg attttttccg atgcctgtag tgatacatta
     1681 cccaaaagca tactcatact aaaaactatt gcaaagaccg tttcaattcg taccttttcg
     1741 gtaatatcag gaaaatattt ttccccggtt atcttcctcc aattcctcat cgcttgcaga
     1801 gctgtcattg tcattggtcg tgctctcaga gtctaattca gtttgccatc cgaaatctat
     1861 ttcgtttatt tcaacatatt caaccaactc tgtcgaatct tcgattggac tatctgctaa
     1921 atcctggata tctgcacgaa attcctcatc atactcattt tcatcggaaa gttctgatga
     1981 ctcttcctct tcatattgtt caattaatgg agaaccaaca taatcctctt gagagaaagt
     2041 agcctgttct tcagtcgttt cttctgctga ttggttatta cgaattattt ctgtttctgc
     2101 aagatcttgt tgaatttgct gtgaggcttg tgcaattgca cgttgatccg ctttcgtgcg
     2161 tgcatcggct gcttcttttg ccatggcttc tacggcttct cttgcgatag cttgttcaat
     2221 cgcaagcgca tcactttcaa ttctagcttg aattgcatta agcgctgaag tctcagcctg
     2281 aactctagca aacgttgttg aagcagctat ttctgttgct ttcaattttt cttctgtttt
     2341 aaccgcagca acaattctgg cctgcgcgat cttggatgct aattctgtca ttctttgctc
     2401 attctcaatg cgagaagtaa tagaaatttt tgcttgcacg attgcatcat gccgtttacg
     2461 agcatccgcc gctaaccgtg cctctatctc tgccagttca attgcagctt cagcagcaag
     2521 cttatccact ttagctctat cttctacttc agctgctgct ttcgcctctg cggccattct
     2581 ttcttccgca gcctgtttcg ctgtcgcttc cgctatcgct cgctcacgtg attttttgat
     2641 tgcctccgcc tgcgctttga tacgctcctt cgttgcttca actgcagcat tatcagcatt
     2701 cattttggca ctaagtgttg ctacctcttg ctgttccgct tgagcacggc tctctgctga
     2761 aaattgagct tccttctccg ctgtaatttt ttgattaatt tcttccaata gccgtgcttc
     2821 agcttgagca cgagaaattg caatagcttc agcttccaac tgagcttttg cggtcgcttc
     2881 cgcatttgct gcggcagcct tatcagcttc agctcgtgca attgctgcct ctgctgcctt
     2941 tgcatcagct tctgcacgta attctgctgc agtttt
//
LOCUS       DAOQLJ010000233         2970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_18857_length_2970_cov_4.749571, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000233 DAOQLJ010000000
VERSION     DAOQLJ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18857_length_2970_cov_4.749571"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PK522_11395"
     CDS             complement(<1..497)
                     /locus_tag="PK522_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNC42379.1"
                     /translation="MNQSLSIWNHLAAAPHRGLFLVGAVQGVFTLLWWVLVLLGRYGF
                     LQITTEWAIAPSWAHAYLMVYGFFPFFIFGFLFTTFPNWMNGEKIKPSQYLSTCIFML
                     IGVILFYSGLFWGISFVLIGIGAMLIGWGVAIVALLTVLFQTKAQDKRHASVASIAFI
                     FGWLG"
     gene            619..1455
                     /locus_tag="PK522_11400"
     CDS             619..1455
                     /locus_tag="PK522_11400"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HNC42380.1"
                     /translation="MLKIDLHCHSTISDGLFTPTQIIEHAVSRNIQVLALTDHDDVAG
                     IEEASMAAREKNITFIAGVEISVTWQNKTLHILGLGVDPQSSPLVNGLSAIREGRIGR
                     AQNIAKQLDKFGIHGSFEGAQTYASKGKLIGRTHFARFLVEKGHAKDVRSVFKKYLVK
                     GKPGYVSHQWASLSDAVNWINSSGGQAVIAHPGRYQLNKRLLESLLNEFQALGGLGIE
                     IVSPSHSPEQIELFARYSNRLNLFASCGSDFHGPGESHFDLGQIPPLPANCTPIWQNW
                     EI"
     gene            1503..2132
                     /locus_tag="PK522_11405"
     CDS             1503..2132
                     /locus_tag="PK522_11405"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNC42381.1"
                     /translation="MAQFFTIHPQNPQSRLLKQAVAILREGGIIVYPTDSCYALGCQI
                     GNKDAMNRIRVIRQVDEHHHFTLVCRDLAEIATYAKVDNSQFRLLKANTPGSYTFILQ
                     ASKEVPRRLQHPKRNTIGLRIPDHPVAHALLAELDEPILSSTLILPGDELPLTDPEEI
                     RHQLEHQIELVLAANSCGIEMTTVIDLTTDTPALIRQGKGSLKPFGLTS"
     gene            2132..2809
                     /locus_tag="PK522_11410"
     CDS             2132..2809
                     /locus_tag="PK522_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNC42382.1"
                     /translation="MENFDSIIQGIAIYALPVIFAITFHEAAHGYIAKHFGDLTAYQA
                     GRISLNPIKHIDPMGTIIVPLTLFIIGKLTMGTGFLFGWAKPVPVNFGQLHHPKRDML
                     WVAAAGPIANLFMACCWAVVLKLGALSFPDNFYAKPMILMGKAGIDINLILMVLNLLP
                     LPPLDGGRMAISLLPNRLAYMFSRIEPYGFMILLFLLITGILGAIVWPFIVFMKYAIA
                     WLFGLYI"
     gene            2853..>2970
                     /locus_tag="PK522_11415"
     CDS             2853..>2970
                     /locus_tag="PK522_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42383.1"
                     /translation="MFADRVLSGMRPTGQLHLGHYHGVLKNWVTLQQQYECLF"
BASE COUNT          907 a          627 c          577 g          859 t
ORIGIN      
        1 atccctagcc atccaaaaat gaatgcaata cttgccacgc ttgcgtgacg tttatcttgc
       61 gcttttgttt ggaacagcac tgttagtaat gcaacaatag ccacccccca cccaatcaac
      121 atagcgccta taccgattaa aacgaacgat attccccaaa ataatccaga ataaaataaa
      181 atcactccga tcaacataaa aatacaagtt gataaatact gactaggttt aattttttct
      241 ccattcatcc aattaggaaa ggttgtaaat aaaaaaccaa agataaaaaa aggaaaaaat
      301 ccataaacca ttagataagc atgtgcccag ctcggagcaa tagcccattc tgtagtaatt
      361 tgcaaaaagc cgtaacgtcc caaaagtacc aatacccacc ataggagtgt aaagacacct
      421 tgcaccgcac ccacaaggaa aaggccgcga tgaggcgcag ccgctaaatg attccaaata
      481 gacaaagatt gattcataaa tacgctcttc agttaaaatg atatttatta tgtgctaagc
      541 aacgattgag ctttctgatt tttcatgaaa tatttttatt tgctatgatg cattcactaa
      601 attgtaacaa ctgttttaat gctaaaaatt gacttgcatt gccattcaac aatttcagat
      661 ggattattta ccccgactca aattattgag catgcagtat cccgaaatat tcaggtgtta
      721 gcactaaccg atcacgatga tgttgcaggg atagaagaag cgagcatggc tgcgcgtgag
      781 aaaaatatca catttattgc tggcgttgaa atttctgtca cttggcaaaa taaaacactc
      841 cacatccttg ggttaggagt tgacccacaa tcttctccat tggttaatgg cttaagtgca
      901 atccgggaag gccgaatcgg tcgcgctcaa aatattgcaa agcaattaga taaattcggt
      961 atccatggga gctttgaagg tgctcaaact tatgcaagta aaggaaaact aatcggacga
     1021 acccattttg cgcgcttttt agttgaaaaa ggccatgcaa aagatgttag gagcgtcttt
     1081 aaaaaatatc tcgtcaaagg aaagcccggt tatgtttctc atcaatgggc ttcattaagc
     1141 gatgcggtta attggatcaa tagcagcggg ggtcaagcag tcattgcaca ccctggccgt
     1201 tatcaattaa acaaacggtt attggaatct ttgctgaatg aattccaagc actaggggga
     1261 ctgggtattg aaatcgtttc cccgagtcat tcacccgagc aaatcgagtt gtttgctcga
     1321 tatagtaatc gcttaaattt attcgcttcc tgtgggtcgg attttcatgg gcctggtgaa
     1381 agtcacttcg atttggggca aatcccccct cttcctgcta actgcacacc aatatggcaa
     1441 aattgggaaa tttagttttt ctttagtcat cattatagta attgagttga ttcactaaca
     1501 aaatggcaca attttttaca attcacccac aaaatccgca atcaagatta ttaaagcaag
     1561 cagtcgcaat acttcgtgaa ggcggtatta tcgtctaccc cactgattca tgttatgcac
     1621 ttggctgtca gattggaaat aaagatgcaa tgaatcgaat ccgcgtcatt cgacaagttg
     1681 atgaacatca ccatttcact ttggtttgtc gtgacttagc tgagattgca acttatgcaa
     1741 aagtcgataa tagtcaattc cggttactca aagccaatac acccggtagc tatactttta
     1801 ttcttcaggc gagtaaagaa gtaccccggc gtctacagca tcccaaacga aataccattg
     1861 gattgcggat tcctgatcac cctgtagcac atgcattact cgcggaactt gatgaaccga
     1921 ttttgagctc aaccctcatt ctaccgggag atgagctgcc actcacggat ccagaagaaa
     1981 ttcgacatca acttgagcat caaatcgaac tagtattggc tgcgaattcc tgtggaattg
     2041 aaatgacaac ggtcattgat ctaacaaccg atacaccagc actgattagg caaggaaaag
     2101 gaagcctaaa gccctttgga ttgaccagct aatggaaaat tttgacagta tcatccaagg
     2161 gattgcaatt tacgcactac ccgtcatttt cgccataaca ttccacgaag cggctcatgg
     2221 gtatattgcc aaacactttg gtgatttgac tgcttatcag gctggacgca tcagtttaaa
     2281 tccaatcaag catattgacc caatgggaac gattattgtc cctctaacct tgtttatcat
     2341 tgggaaatta accatgggaa caggcttttt attcggttgg gcaaaaccag ttccagtcaa
     2401 ttttggtcaa ttacaccatc cgaaacgcga tatgttgtgg gttgctgcag ccggacccat
     2461 tgcaaatctt tttatggcat gctgttgggc agtcgttcta aaattaggtg cactctcttt
     2521 tcctgacaac ttttacgcaa aaccgatgat attaatgggt aaggcaggga tcgatatcaa
     2581 ccttatctta atggtgttaa atctattacc cctacctcca ctcgatggtg gccgtatggc
     2641 aattagctta ctcccaaacc gactagcata tatgttttcg cgtattgaac cctacggctt
     2701 catgattctt ttatttttgc taatcaccgg tatactggga gccattgttt ggccttttat
     2761 cgtattcatg aagtatgcga ttgcatggtt atttggctta tatatttaat gagaaatttc
     2821 aatctatcac gaattaattt ggaaaattaa tcatgtttgc tgatcgtgtt ttatcaggta
     2881 tgcgtccaac aggtcagtta catttgggac attaccatgg cgttctaaag aactgggtaa
     2941 ctttacagca acaatatgaa tgtttgtttt
//
LOCUS       DAOQLJ010000234         2968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_18883_length_2968_cov_6.496395, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000234 DAOQLJ010000000
VERSION     DAOQLJ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18883_length_2968_cov_6.496395"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PK522_11420"
     CDS             complement(<1..289)
                     /locus_tag="PK522_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42384.1"
                     /translation="MVKIMSLIVWGFLMISVSQVAWAHSEEQLKSVETPHGRQLRMAG
                     PFHLELVAKDNELTLYITDHGNKPIGSAGGQGKANIIIASEKDGKKQSVNLE"
     gene            complement(355..2385)
                     /locus_tag="PK522_11425"
     CDS             complement(355..2385)
                     /locus_tag="PK522_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNC42385.1"
                     /translation="MIESIAAISRWAQLVANLVLFGSCIFLLMASSQNSIFKYPWVAK
                     LEMLFPKLAIVVLIGLVGMLATTTGQATGIPSDAWQPMVWFEIVKETRMGHLWVARAI
                     SAVIMLLVFIYILRLHRTKWHYLLGAIVAALPLIAGSLVSHSAAEEISFVSIFPYSLH
                     ILLAGAWFGALPAFLLIIFTSRKKETNDKSLDGSGIDGLKQFSKIALPVMLAIVATGF
                     VIANRMVEEDYHALVSSSYGWLLNIKLALLAVILLIAYQARSKWLPLFTSVNNPEETV
                     KGNQLLWKWVRVEFVLAIVLVLFATILANTIPAKHAIIDNWPYPFRLSVDATWSEANV
                     QPLFWGGAALLLLAVGIAWYAVSNRWEMKRRILIPGILGLSSLAIALPPLAVDAYPET
                     YRNTPVPFDVISISNGAALYAKNCVSCHGPQGKGNGIIAKTFNPPPADLLTEPHTARH
                     TAGDFFHWLTYGIKNTGMPGFSGNLSEEDRWDVVNYMHGMSRGYQGRILNSKVIPEKP
                     SLGPPIFPYFAHDGSSGVIKDFRQKNNVLLVLFSWPQSQERIHELTEAYDHLKKLNTI
                     VLAVPMGNLNKQILAEITKDLPFPVVTEGAQEIVDSYVLYRRTLTHPDILGEGNIPDH
                     LEFLIDRFGYLRARSIPAVDELTWSDMNLLSQQLSQLNQEDEILPPPKDHVH"
     gene            complement(2437..2835)
                     /locus_tag="PK522_11430"
     CDS             complement(2437..2835)
                     /locus_tag="PK522_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HNC42386.1"
                     /translation="MKGIAVGRSNRNLSWVVLLIVSIVCFFNISSVFAHAMLVKAEPA
                     KRASLTTAPSQVRLWFNEEIEPAFAALSVLDANKKAVTEDKAVVDSEDPKSIILKLPE
                     MQSGRYTVKFRVLSVDGHVVDSEYEFTVKE"
BASE COUNT          902 a          671 c          527 g          868 t
ORIGIN      
        1 gttccagatt aacagattgt ttcttaccat ccttctcact cgcgatgata atatttgctt
       61 taccttggcc acctgccgat ccaatcggtt tattaccatg atcggtgata tatagcgtta
      121 attcattatc ttttgcaacc agctcaagat gaaacggtcc tgccatacga agctgcctac
      181 catgcggcgt ttcaactgac tttaactgct cctcactatg agcccaagct acttgagaaa
      241 cactgatcat taagaaaccc caaacgatga gagacataat ttttaccatt ttcttatcct
      301 cttgatttta atcattagaa aacaacatta ttgcatagca attcataata agagctaatg
      361 cacatggtct ttgggtggtg gaagaatctc atcttcctga ttcagttgac tcaattgctg
      421 tgacaataag ttcatatctg accatgttaa ttcatcaact gctggaatcg agcgagcgcg
      481 caaataaccg aaccggtcaa tcaaaaattc taaatgatca ggaatattcc cttcacccag
      541 aatatctgga tgggtcaatg ttcttcgata taaaacataa ctatccacaa tttcttgtgc
      601 accttccgtc actacaggaa aaggtagatc ttttgtaatt tccgctagta tctgcttatt
      661 cagattaccc attggaaccg caagtactat cgtattcaat tttttcaaat ggtcataagc
      721 ttctgtcagt tcatgaattc tttcttggga ttgtggccaa gaaaataaca ccaacagcac
      781 attatttttc tggcgaaaat ccttgatgac gccactactt ccatcatggg caaagtaagg
      841 aaatatcggt ggacctaatg acggtttctc cgggataact ttggaattaa gtattcttcc
      901 ctgatatcca cgcgacattc catgcatata attcaccaca tcccaacgat cttcctccga
      961 taaatttccc gaaaagcctg gcatccctgt atttttaatc ccataggtca accaatgaaa
     1021 aaaatcaccc gccgtatgcc ttgcagtatg gggttctgtc aataaatctg ctggaggagg
     1081 gttaaatgtt ttagcgataa ttccattgcc ttttccttgt ggaccatgac agctcacgca
     1141 gtttttggca tataacgcag cgccattaga aatagaaatc acatcgaagg gaacgggcgt
     1201 atttcgataa gtttccggat aagcatcaac cgcaagcggt gggagcgcga tggcaagtga
     1261 tgataaccct agaatccccg gtattagtat tctacgcttc atttcccacc gattactaac
     1321 ggcataccaa gctattccga ccgctaacaa aagtaaagct gctccacccc agaataaagg
     1381 ctgtacattg gcttcactcc atgttgcatc cacagacaaa cgaaatggat aaggccaatt
     1441 atcaataatg gcatgctttg caggaatcgt attcgctaga atcgtagcaa acaataccaa
     1501 cacaatagcc agtacgaatt caactcgaac ccacttccat aataattgat taccttttac
     1561 cgtttcttct ggattattta ctgaagtaaa taacggaagc cattttgagc gcgcttgata
     1621 ggcaatcaaa agaataaccg ccaatagcgc taacttaatg tttagtagcc atccataaga
     1681 actcgacaca agggcgtgat aatcttcttc caccatacgg tttgcaataa caaaaccggt
     1741 agccacgatt gctaacatga ctggcagtgc aatcttagaa aattgcttta atccgtctat
     1801 accggatcca tcaagtgatt tatcgtttgt ttctttcttg cgtgaagtaa aaataattaa
     1861 taaaaatgca ggtaatgcgc caaaccatgc acccgctagc agaatgtgca atgagtaagg
     1921 aaaaatggaa acaaacgaaa tttcttcagc cgccgaatgg ctaacgaggg agcctgctat
     1981 caagggtaat gctgctacaa ttgcacctaa tagataatgc catttagttc tatgcaatct
     2041 caaaatataa ataaaaacta atagcattat cactgctgaa atcgccctag ctacccataa
     2101 atgccccata cgggtttctt taacaatttc aaaccaaacc ataggctgcc atgcatcaga
     2161 tggaattcca gtcgcctgcc ccgtggttgt tgcaagcatc cctactaatc caattaatac
     2221 aacgatagct aattttggaa aaagcatttc caatttggcc acccatggat acttgaatat
     2281 cgaattctga ctactggcca tcagcaggaa aatgcaacta ccgaatagaa ccagattggc
     2341 tactagttgt gcccaacgtg aaattgctgc aattgactca atcattattg ttatttttta
     2401 actttctttt tttgatgcgg cgctattctt tctcagttat tcctttactg taaactcata
     2461 ttctgagtca accacatgac catcaactga caatactcta aatttgacgg tataacgacc
     2521 tgattgcatt tcaggcaatt taagaatgat agacttcgga tcctctgagt cgactacagc
     2581 tttatcctcg gttactgcct ttttattcgc atcaagtaca gataatgctg caaatgcagg
     2641 ctcgatctct tcattaaacc aaaggcgcac ttgtgaaggg gctgtcgtaa gtgaagctct
     2701 ttttgcaggc tcagctttaa cgagcatggc atgcgcaaat acagaactta tattgaagaa
     2761 acatactata ctgactatca gtaacactac ccaactgaga tttctatttg atcggcctac
     2821 tgctatcccc ttcacgttgt tgctccttat tttagtttta ctaataaaca ataattagag
     2881 tagttttatt taagagcgtt cgattttttc ctcacgcttt tttattagat ttaaggaaga
     2941 agaaggcgat accggcagcg attgcaag
//
LOCUS       DAOQLJ010000235         2864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_20089_length_2864_cov_4.096120, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000235 DAOQLJ010000000
VERSION     DAOQLJ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20089_length_2864_cov_4.096120"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1867
                     /locus_tag="PK522_11435"
     CDS             <1..1867
                     /locus_tag="PK522_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HNC42387.1"
                     /translation="DSGDPGNSIAGASPSGAIFLNPSDDPGLVTFIVSDEPGSWFRSA
                     RGDALSPVTFTKSLEVIPPGSSVHFVMSDTNGVHTITSLLWPSEAGNNNHPGGDHFMI
                     PFDQTKAYRGGGIVQLNVPGLYVFTCKVHPYMFGAVIVDSPGTTGLDLGNAIDLVSGI
                     KNLPTSSDLATRLLRTFFVATTQGNWQDYTAPTPWHVNYPNVPVETDLGTLNLKDVLE
                     ARYGQDVVLPALTAPTIPGVGEVWINTQFEKTAGKTKPGTITVLDTATWTLKRKIALP
                     EINMNNPHNVWPNRDHTIMYQTQWFDTKLTLIDQKTGKLIKNLQVGDTPSHVVTLPNS
                     DDVTVAINGENGVVIIPAGTTEVNYMMPTQHHGQVPANPHGHWVSADGSRIITPNINT
                     SDTGIYNADTGQIVSRTQTGNGAPGPHPIAIGMMPDSSKIYASNLLHHSLSVLDGNNG
                     ALLKTINLIENYNPITGDITGPVGVLPIQTPVSPDGKAVVLAATGGQILVIDTKTDSI
                     VKMFACDAGCHGANFGAKQGGGYYAYVTSKFSNRLIVVDIDPNNDGDISDADIVGSIS
                     MVAGNNVAKDDTISSLAGYGGQGIVALPNVYNGWVQNLTTEWASGLTSSQRNPVQ"
     gene            complement(2006..2626)
                     /gene="metW"
                     /locus_tag="PK522_11440"
     CDS             complement(2006..2626)
                     /gene="metW"
                     /locus_tag="PK522_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HNC42388.1"
                     /translation="MIMTEITDTISPQLRPDFATIAAWIKPATKVLDLGCGDGALLRY
                     LTDTRQIKGYGVEIDDTNILACLNNRINVIQSDLESGLSGFESNSFDYVILSQTLQAM
                     KHTENIIREMLRVGKEGIVTFPNFGHWKNRLQILRGFMPVSPTLPYEWFDTPNIHLCT
                     LKDFEQFCHQHGVHILEKRVMSDNQQTTFAPNLFGMLAFYRFTQRK"
     gene            complement(2668..>2864)
                     /gene="metX"
                     /locus_tag="PK522_11445"
     CDS             complement(2668..>2864)
                     /gene="metX"
                     /locus_tag="PK522_11445"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964716.1"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNC42389.1"
                     /translation="LVLSFSSDWRFSPERSRSIVKALLDNNLNVSYAEIASRHGHDSF
                     LMKDDHYHQLVRSYMNNITT"
BASE COUNT          804 a          599 c          690 g          771 t
ORIGIN      
        1 agactcaggc gacccaggta acagtattgc aggtgcttca ccgagtggtg ctattttctt
       61 gaatccgagc gatgatcctg gtctcgttac ctttattgtt tctgatgaac ccggaagctg
      121 gtttagaagt gcgcgcggcg atgcattgag cccggtaacg tttactaaat cgctggaagt
      181 cattccaccg ggaagtagcg ttcattttgt tatgtccgat acgaacggtg ttcacaccat
      241 taccagctta ttgtggccaa gcgaagccgg aaacaataac cacccgggtg gcgatcattt
      301 catgattcca tttgatcaaa ccaaagcata tcggggtggt gggatcgttc aattgaatgt
      361 tccagggtta tatgttttta cctgtaaggt tcatccttac atgtttggtg cagtcatcgt
      421 ggatagccca ggaacaacgg gtttggatct cggtaatgcc atcgatttgg tatcaggcat
      481 caaaaattta ccgacgagta gtgatttggc tacccgatta ttgagaacct tctttgttgc
      541 cacgacgcaa ggaaattggc aagactatac cgcgccaact ccttggcatg tcaattatcc
      601 caatgtacct gtcgaaactg atttgggaac gctgaacttg aaggatgtac ttgaagcaag
      661 atatggacag gatgtcgtcc tacctgcttt gactgctcca acaatcccag gggtgggtga
      721 ggtatggatt aacacgcaat ttgagaaaac agctggaaaa acaaaaccgg gaaccattac
      781 ggttttggat acagctactt ggacactcaa gcgtaaaatc gcattgcctg agatcaatat
      841 gaataatccc cacaacgttt ggccaaatcg tgatcatacg atcatgtacc aaactcagtg
      901 gtttgataca aagttgacat tgatcgatca aaaaacaggt aagttgatta aaaaccttca
      961 agtgggagat acgccttcgc atgtcgtgac attaccgaat tcggatgatg taaccgttgc
     1021 gatcaatggt gaaaatgggg ttgtcatcat tccggcagga acgaccgaag tcaattatat
     1081 gatgccgact cagcatcatg gtcaagtacc tgcaaatccc catggacatt gggtatcagc
     1141 cgatgggtct cgtatcatca cccctaatat caataccagc gataccggta tttataatgc
     1201 agacacaggg caaatcgttt caagaacgca aacggggaat ggtgcacctg ggccgcatcc
     1261 cattgcaatc ggtatgatgc cggattcaag caaaatatac gcttcaaacc tgctccatca
     1321 tagtttgagc gtcttggatg gaaataatgg ggcattatta aaaaccatta atttgattga
     1381 aaattacaat ccgatcaccg gtgatattac aggccctgtc ggtgtacttc caattcaaac
     1441 gcctgtttct ccagacggta aagcggtggt tctggctgca acgggcggtc aaattttggt
     1501 tatcgatacc aaaaccgaca gcatcgtcaa aatgttcgcg tgcgatgctg gttgtcatgg
     1561 tgccaatttt ggcgccaaac aaggcggtgg atactatgct tatgtaacca gcaaattttc
     1621 taatcgcctg atcgtagtgg atattgaccc aaataacgat ggtgatatat cggatgcgga
     1681 tattgttgga tccatctcca tggtggcagg caacaatgtc gcgaaggatg acaccatcag
     1741 cagtttggca ggttatggtg gccaagggat tgtagcgcta cctaacgtgt acaatggctg
     1801 ggtacaaaac ctaacgactg aatgggcttc aggtttgacg agcagtcaac gtaacccggt
     1861 tcaataagca gcaactcgcg attagttgat tcgcaatagc cgatgaccag attcatagtt
     1921 tggtcatcgg tttttttgtt ttgtgagcag ttaaagaatg aaggtttaag ctttttcaga
     1981 tagtgggtag agagaggaga gctttctact ttctttgtgt aaaccgataa aaggcaagca
     2041 ttccaaacaa gttgggtgca aatgtggttt gctgattatc tgacatgact cgtttttcta
     2101 aaatgtgaac cccatgttga tggcagaatt gctcgaagtc tttgagtgtg caaagatgaa
     2161 tattcggtgt atcaaaccat tcatagggca gtgtcggtga aaccggcata aagccccgca
     2221 ggatctgtaa acgattcttc caatgaccga aattagggaa tgttacgatg ccttcttttc
     2281 ccacacggag catctcacga atgatattct cggtatgctt cattgcttgc agcgtttggg
     2341 acagaataac gtaatcgaat gaattggatt caaagccaga taagcccgat tctaggtcac
     2401 tttgaatgac gttaatccgg ttattcaggc aggccagaat attcgtatca tcaatttcca
     2461 caccgtatcc tttgatctgg cgtgtatcag taagatagcg taatagcgca ccatcgccgc
     2521 aaccaaggtc gagtacttta gtcgcgggct taatccaagc tgcaatcgtg gcaaaatcag
     2581 gtctaagctg aggagaaatg gtatcggtta tttcagtcat gatcattgaa gtcaaagcta
     2641 cccgtaatta agcgagtcga tgcgcgatta agtcgtgatg ttattcatat aagaccgtac
     2701 taactggtga tagtggtcat ccttcattag aaacgaatca tggccgtggc gagaagcgat
     2761 ttcagcgtag ctaacattga ggttgttatc cagcaatgct ttcacgatgc tccgtgagcg
     2821 ttcaggcgag aaacgccaat ctgagctaaa tgaaaggact aaaa
//
LOCUS       DAOQLJ010000236         2863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_20100_length_2863_cov_8.353632, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000236 DAOQLJ010000000
VERSION     DAOQLJ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20100_length_2863_cov_8.353632"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PK522_11450"
     CDS             complement(<1..281)
                     /locus_tag="PK522_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HNC42390.1"
                     /translation="MSESTLSNFINLLVYSLATVMGLVVVIAIVALFIRDITQKQHTL
                     LRNYPIIGRLRYFFEIQGKYFRQYLFSGDRDEMPFNRATRTWVYKSAKN"
     gene            complement(719..1285)
                     /locus_tag="PK522_11455"
     CDS             complement(719..1285)
                     /locus_tag="PK522_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42391.1"
                     /translation="MIKQSIQLTISTCILVLCYVGTVFGHGKVPLEDDVCVRMAGGNM
                     VHLSTYQPQFDVNEQYCTEIPTEGETYLVVDLLDPALRNMPVSVKVFRGGSEDGETVA
                     QINADYYSDGVINGIGRLDKGLYSVVVTAEGVPPLNYHYQLRVAMLDYGKVARSMIGP
                     MIAMLILSWIAYRFLQSRRIRFPFNLRQ"
     gene            complement(1697..2254)
                     /gene="efp"
                     /locus_tag="PK522_11460"
     CDS             complement(1697..2254)
                     /gene="efp"
                     /locus_tag="PK522_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNC42392.1"
                     /translation="MKTAQELRVGNVFMLGKDPMVVLKSEFTKSGRNSSVVKMKFKNL
                     LTESPSEAVYKADDKFDIVVLDKKEVTYSYFAEPMYVFMDAEYNQYEVEAETMVDALN
                     FLEDGMPCEVVFYNGKAISVELPNTVVREIVYTEPAVKGDTSGKVMKNAKITTGFELQ
                     VPLFCEIGEKIEIDTRTLEYRSRVK"
     gene            complement(2422..>2863)
                     /locus_tag="PK522_11465"
     CDS             complement(2422..>2863)
                     /locus_tag="PK522_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42393.1"
                     /translation="NFKSRGKQAMPLNDGEEPKLKPLGPGGGEVQEKEKALIAEIIAK
                     VNDLFEGDLTDDDQLIYVNNVLKGKLLESEILIQQAMNNTKEQFSNSPDFSKEFMNAI
                     MDALAAHSTMSRQALDSERVRSGLKDILLGPAKLYEALRERQQS"
BASE COUNT          860 a          576 c          547 g          880 t
ORIGIN      
        1 ttattttttg cactcttata aacccatgtt ctagtagcac gattaaaggg catttcatct
       61 ctatcacctg aaaacaagta ttgccgaaag tatttccctt gtatctcaaa gaaatatcgg
      121 agtcgaccaa tgattggata gttgcgcaac agcgtatgct gtttttgggt aatatcgcga
      181 ataaacagcg ctacgattgc gataacgaca actaacccca tgactgtggc taagctataa
      241 accagtaagt tgatgaaatt tgataaggta gattcagaca tgtcttcaga aataagatgt
      301 ggtaatgaca gctatttttt tagagaaaaa gatagccata gattaacgcg caacaagcat
      361 ttgataaata cgtggattaa atttatcaat aatgatgata gagaattgct taggaatgat
      421 gggttgatac tcaaacaacg ggcgaggcgc ttttcctcaa tagtttgtac caaatgaaag
      481 agagggtatt ggaaataatt aaaccaatac aagcggtggg gggcgatgat gtatctatga
      541 atggattggt tagcccatta gctatcccga ttgccgagct aaatagctca ttgattttgt
      601 ttctttagta attttatctg gaaactaggg gacactaaac gttgttaaaa aggaaacttt
      661 gtctaaggat tattccaccc tctgctaaat tgtcagaggg tgatagagcg taaatggttt
      721 attggcgtaa attaaaggga aatctgatcc gccttgactg taggaagcga taggcaatcc
      781 aagatagaat caacattgca atcattgggc caatcatact tcgggctact ttgccataat
      841 caagcattgc tacacgaagt tggtaatgat aattcaaagg cggcacaccc tcggctgtaa
      901 caacgaccga ataaagccct ttatccaacc tgccaatacc attgatgacg ccatctgagt
      961 aatagtcagc atttatttgt gcgacagtct ctccatcttc tgagcctcca cgaaatactt
     1021 tcacacttac tggcatattt cttaaagcag ggtcaagtaa atcgacgaca aggtaggttt
     1081 cgccttctgt gggaatttca gtgcaatatt gttcattcac atcaaactga ggttgataag
     1141 tactgagatg aaccatgttt cctccagcca tgcgtacaca aacatcatct tctagtggaa
     1201 ccttaccatg accaaaaacg gtacctacat aacatagaac aaggatgcag gtcgatatag
     1261 ttaattgtat tgattgtttt atcacgacaa ccctcctgtg tttatcaaaa attttctgtt
     1321 gttgccaatt taagtgtttt tttcatttgt attccatttc atggatctac aatgagatcg
     1381 gacagcatga gttaaatttt gttcatttag gtaagaatca aaacttaaaa ttactttaac
     1441 gggcggttgt aaaattacct acgatcccgc tgcaaaacag acttaaatac gctatttatt
     1501 agatataacc tgcgcttttt tatctgtttc tgccctaaga ttgacttcct cagaaatgtt
     1561 tttcaacagt ctgttagaac ccaaagatag gtgtagcaat gcaaaaataa aaaaactttt
     1621 gttggatttt aagatttggt tttagcgaaa aaaaaggagc cattgggctc cttttttaag
     1681 aacagatagc gagaacttac ttaactcgac tccgatactc cagtgttctg gtatcaattt
     1741 caattttttc accaatttca caaaacagag ggacttgcaa ttcaaagcct gtagtaattt
     1801 tagcgttttt cataacctta ccggaggtat caccctttac agcgggctcg gtataaacga
     1861 tttcacgcac gactgtgtta ggaagttcaa ccgaaatagc cttgccatta taaaatacga
     1921 cttcacaagg cattccatct tctaaaaagt taagtgcatc taccatggtt tctgcttcaa
     1981 cttcatactg attatattca gcatccatga aaacgtacat tggttcggca aagtaagaat
     2041 acgtaacttc tttcttatct aaaacaacga tatcgaactt atcatctgct ttgtaaaccg
     2101 cctcgctggg tgactcagtc agaagattct tgaatttcat tttaacgact gaggaatttc
     2161 gacctgattt ggtgaattct gatttcaaca caaccatggg gtcttttcca agcataaata
     2221 catttccaac acgaagttcc tgagctgttt tcatctagat gaatgcaatg agtagaatta
     2281 aaaacagaat tatacgctga gtgagtcaaa aaaatccagt tgtgttatag ctataaaaag
     2341 atagcttata acttagatta agctttatat ttcaatgaag tagcttaacc tttgcaaagc
     2401 atggatttgt cgttatttct tttatgactg ttgccgttcc cgcaacgctt cataaagctt
     2461 tgcaggcccc agcaatatat ccttcaaacc gctgcgtacc cgctccgaat ccagcgcctg
     2521 cctgctcatc gtgctatgtg ccgcaagagc atccataatt gcattcataa attctttgga
     2581 gaaatcagga gagttcgaga attgttcctt ggtgttattc attgcctgtt gtatcaggat
     2641 ttccgattcc agcaatttac cctttagcac attattgaca taaatcagct ggtcatcatc
     2701 ggtcagatcg ccctcgaaca ggtcattgac cttggcgatg atttcagcga tcagcgcttt
     2761 ttccttttcc tgcacctcgc cgccaccggg gccaaggggt ttaagctttg gttcctcccc
     2821 atcattgagc ggcattgctt gtttaccgcg actcttgaag ttg
//
LOCUS       DAOQLJ010000237         2849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_20294_length_2849_cov_4.985684, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000237 DAOQLJ010000000
VERSION     DAOQLJ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20294_length_2849_cov_4.985684"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..>2849)
                     /locus_tag="PK522_11470"
     CDS             complement(223..>2849)
                     /locus_tag="PK522_11470"
                     /inference="COORDINATES: protein motif:HMM:NF039478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HNC42394.1"
                     /translation="LEDSLIREVALGGPDTATREKTLWQVKLLRAADLSTNLNCLSAN
                     TEWDTLIANPDGQLAARAEPSTPPKDPCLLAPEAGYRRLENQLYRVEIHDDGTLVKPR
                     FKWSRNNGFLETKVVRWLNDPVADEFKVASIGRDAFLAITAGCWVEFFDETHELLGQP
                     GTLVKVLKTAGNVVTVDLATKTGSLDKALFNDNPRVRVWENWADIVTGLPSSNTGWVE
                     LEDGVEIKFTPGKYRSGDFWMIPARTAKADIEWPKEGNKPKFLSPQGILRVFTRLALF
                     SVSAGNWTFLSDCRSLFPALSELTNLYYVGGDGQEIIPDFVTPANNKLPSPLEVAVFN
                     GEFPVTGAQVRFVASHGTFPNGTQTQIVSTAANGISSIDWTLSPNNLNQTVTAELLEA
                     GQSAVGKYTKIHFSARLSTACQVAYNPGQCNDLKVLGVTNVQDAIDALCQRIRSNTGC
                     CVTVGRDGEFPALDIAIKALSEKYSEICICLLPGNHHLTESINLQAQGLKLLIHGAGR
                     GSQLILEGGKFNFMDLDALSLRDFDILNIGDPIRLRVQGTAEVQLHNMHISGMSRPGL
                     SLVQISEAEDIQISSSRLNTFQKEVTDTFKQVLSAIDLLKPLSASFNLKNGDIFQPVS
                     REVVAEFAKYDEHTRNALIKSIQAFIRQSAASSTISEESKQSLLTLAETFVSEPNTRT
                     LSANLNAVRATLILNRPGFALAIEDAQAKTLLADNQINGRFSIYGESSNIEMSTEILN
                     ADFRKLLQAEIASGRIVLKPARGHLRLRNNVMREIRFGDEFLNFLLFIINSGRGELKN
                     CYANLIADANTLVGRDSQLIAIDLAFNGNVLQPFKDVGIVIATQAKYIGNFAHDDFRL
                     FNVGNNPENFGNGRLNIV"
BASE COUNT          760 a          685 c          601 g          803 t
ORIGIN      
        1 agattcgggt ttgataaagc cgccagattt cctggcgatc caataaggtt aagtaagtgc
       61 gtttatgtat attcatctac agtattctct aaaaatactg tgaacaacac gagagattct
      121 tacagcagca gcttataaat agtgtatgaa tgaatcaata cattatcatc aaagcacgag
      181 aataattgta ttaatctaga aaatgcaatt cgtatcaacc aactaaacaa tatttaaacg
      241 gccattgcca aaattttccg ggttattgcc aacattaaat aacctgaaat cgtcatgcgc
      301 aaaattgcca atatatttag cttgtgtggc aatgacaatt cccacatcct taaatggttg
      361 gagaacattt ccattaaagg ctaaatctat tgctattaat tggctatctc tgccaaccag
      421 cgtattggca tctgcaatca agtttgcata acaatttttt aactctcccc tgccactatt
      481 tatgataaag agcaaaaaat tcagaaattc atcaccaaaa cggatctccc gcattacatt
      541 attgcgcagt cgaaggtgac ctctagcagg cttcaacaca atccttccgc tcgctatttc
      601 tgcttgcagc agcttgcgaa agtctgcatt caaaatttca gtgctcattt caatgttcga
      661 cgattcaccg taaatcgaaa accggccatt gatttgatta tccgctagca aagtttttgc
      721 ttgtgcatcc tcaatggcca gtgcaaaacc gggccggttc aaaatcaatg tcgcgcgaac
      781 cgcattcagg tttgcactca aagtacgagt attaggttca ctgacaaacg tttctgccaa
      841 agttagcagc gactgctttg attcttctga aatagttgag gaagcggcac tttgtctgat
      901 aaatgcctga atactcttta ttaatgcatt gcgggtatgt tcatcgtact tggcaaattc
      961 tgctaccact tcccgtgaaa ccggttgaaa aatatcgccg ttcttcagat tgaagctggc
     1021 acttagcggc ttcaacaagt caatggctga tagtacctgt ttaaaagtat cggttacttc
     1081 tttctggaaa gtattcaagc gacttgaaga aatctggata tcttctgctt cactaatttg
     1141 cacaaggctt aatcccggcc ggctcatgcc ggaaatatgc atattgtgaa gttgcacttc
     1201 cgccgtgccc tgtacgcgca agcgaatcgg atcgccgata ttcagaatgt caaaatcgcg
     1261 tagcgataag gcatccagat ccataaagtt gaacttcccg ccctccagta ttagttggct
     1321 cccacgccct gctccatgaa tcagcagttt taacccttgg gcttgcaaat ttatgctttc
     1381 ggtaagatgg tggttgccag gtaacaggca aatacatatt tctgaatact tctcactcag
     1441 tgcttttatt gcaatgtcca gtgccggaaa ttccccatcc ctaccaacgg taacgcagca
     1501 acctgtgtta cttcggatac gctgacatag cgcatcgatc gcatcctgca catttgtgac
     1561 acctaatacc ttcaagtcgt tgcattgccc tgggttatat gcaacctgac aggctgtcga
     1621 caagcgtgct gaaaaatgaa ttttggtata tttgccaaca gcgctttgcc cagcctcaag
     1681 caattctgct gtaacagttt ggttcaggtt atttggactc agtgtccaat cgattgaaga
     1741 aataccgtta gctgcagtac tgacgatctg tgtttgtgtg ccattaggga acgtgccatg
     1801 actggccacg aatcggactt gtgcaccggt gactggaaat tcgccattga acaccgctac
     1861 ttccagcggg cttggcaatt tgttattggc cggggtaaca aaatctggaa tgatttcttg
     1921 tccatctccg ccaacgtagt acaggtttgt caattcactc agtgccggaa acaatgaacg
     1981 gcaatctgat aagaaggtcc agttgccagc tgatactgag aataatgcta ggcgcgtaaa
     2041 cactcgtaat ataccttgag gcgacaaaaa cttaggtttg ttgccttctt taggccattc
     2101 gatatctgcc ttggcagtac gtgccgggat catccagaaa tcacctgagc ggtattttcc
     2161 aggcgtgaat ttaatctcga cgccatcctc caattctacc catccggtat tgctgctagg
     2221 taatcccgtg acgatatcag cccagttttc ccaaacacga acccgtggat tatcattgaa
     2281 taatgctttg tccagactac cggttttcgt tgcgagatcg acagtgacca cattaccagc
     2341 tgttttgagc accttgacca gagtgccagg ttgacccagc aactcgtgtg tttcgtcaaa
     2401 aaattcgacc cagcatcctg cggttatcgc aagaaaggca tcacgcccaa tgctggctac
     2461 tttaaactca tctgctacag gatcgttcag ccagcgcaca actttggttt ccagaaagcc
     2521 attgtttctg gaccatttaa aacgcggctt taccagcgta ccatcatcat gaatttctac
     2581 cctgtacaac tgattttcca aacgacggta tcccgcctct ggcgccagca ggcaaggatc
     2641 ttttggtggc gtgctgggtt cagcgcgagc agcgagttgc ccgtctgggt tcgcaatcaa
     2701 ggtatcccat tcggtgttgg cggatagaca gtttagattg gtgctaagat ctgcagcacg
     2761 cagcaatttg acttgccata gggttttttc acgggtcgcg gtatcagggc cgcccagtgc
     2821 cacctcacgt atcagtgaat cttccagtg
//
LOCUS       DAOQLJ010000238         2823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_20635_length_2823_cov_6.451228, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000238 DAOQLJ010000000
VERSION     DAOQLJ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20635_length_2823_cov_6.451228"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1899)
                     /locus_tag="PK522_11475"
     CDS             complement(<1..1899)
                     /locus_tag="PK522_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNC42395.1"
                     /translation="MPNHLIHETSPYLLQHVDNPVEWHPWNKEALELAKKLNKPILLS
                     IGYSACHWCHVMANESFQDAEVAAAMNQYFVNIKVDREERPDIDQIYQTAHHMINQRH
                     GGWPLTMFLTPEQKPFFGGTYFPKVSRHGMPGFLELLPKIAETYRTRTDDIEKQNTAL
                     LKLLADTLPTTGAKIPVISRQLILRALTQLDENFDREYGGFGQAPKFLHPAELLFCLH
                     CYFYDSDAGALRIVKHTLEKMAKGGIYDQLGGGFFRYSTDQYWRIPHFEKMLYDNGPL
                     LQLYTDIWLATADPLYKQIVEETASWAIREMQSSYKQGEGGFYSSLDADSEHEEGKFY
                     VWSKEEIERGLSADEFSVVNPYFGFTRFPNFESKYWHLEVSQSVTSVAQQTNCSEDNV
                     QRRVDSARDKLLEIRSQRIRPGRDEKILTSWNALMIKGLTRAGVFFQCEAWIEAAQHA
                     IDFIHGNLWKDNRLLATYKDGKAHLNAYLDDYAFLLDSLLEMLQVQFRQTDFEFAIAL
                     AEVMLDQFEDKHLGGFFFTSHDHEKLIHRPKVGSDNAIPSGNGIAVIALQRLGYLLGE
                     SRYLQAAERALNLFYSEMLHHPSIYCSLLVALEEWLTPPKMIILRGDETRMKEWWRQI
                     KSQTPYTFV"
     gene            complement(1901..>2823)
                     /locus_tag="PK522_11480"
     CDS             complement(1901..>2823)
                     /locus_tag="PK522_11480"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNC42396.1"
                     /translation="LITRKSLENAISVVMAVGGSTNAVLHLLAIAHAAEVELDINDFE
                     VIRARVPVICDLKPSGQYVTADLHKAGGIPQVMKMLLNNGLLHGDCLTISGETIEQVL
                     HNVPDQPPQNQKIIRQWGDPLYEQGHLAILKGNLSPEGAVAKISGVKNPKITGPARVF
                     DSEESCMSAILAREIHAGDVVVIRYEGPKGGPGMREMLSPTSALIGEGLGDSVGLITD
                     GRFSGGTYGMVVGHVSPEAFVGGVIALVREGDSITIDATQRLLQLNISEDELEKRRAA
                     WQPPKARYHRGVLAKYAKLVSTASRGAITD"
BASE COUNT          801 a          677 c          533 g          812 t
ORIGIN      
        1 aacaaaagta tagggtgttt gtgatttaat ttgccgccac cattctttca ttctggtttc
       61 atccccgcga agaataatca tttttggcgg agttaaccac tcctctaggg ccaccaacag
      121 gctgcagtaa atactgggat gatggagcat ttcagaataa aacaaattca gggcacgctc
      181 agcagcctgt aaataacggc tttccccaag taaataaccc agtcgctgca atgcaattac
      241 agcgattcca tttcctgaag gtatcgcatt atctgaccca actttagggc gatgaattag
      301 cttctcatga tcatgacttg taaaaaaaaa tcctcctaaa tgcttatctt caaattgatc
      361 aagcattacc tcagcaagtg caattgcaaa ctcaaaatct gtctgacgaa attgaacttg
      421 gagcatttcc aacaaactat ccaacaaaaa tgcataatca tcgagatagg catttaagtg
      481 cgctttacca tccttataag ttgcaagcaa tcgattatct ttccaaagat taccatggat
      541 aaaatcaata gcatgctggg cggcctctat ccatgcctca cattgaaaga aaacaccagc
      601 acgcgttaat cctttaatca tcagcgcgtt ccagctagtc aatatctttt catcacgacc
      661 tggacgaata cgttgactgc gtatttctaa taacttatcc cttgctgaat cgacccgccg
      721 ctgcacattg tcttctgaac agttggtttg ttgcgcaacc gaggtaaccg attggcttac
      781 ttctaggtgc cagtatttgc tttcgaagtt agggaagcga gtaaagccga aataaggatt
      841 tactaccgaa aattcatcgg cagataatcc cctttcaatt tcttctttac tccaaacata
      901 gaattttcct tcctcatgtt cagaatctgc atctaaggat gaataaaaac cgccctcacc
      961 ctgtttataa ctcgattgca tttcgcgaat tgcccaactc gccgtctctt caacaatttg
     1021 tttatacaat ggatcagccg ttgcaagcca tatatcggta taaagttgca ataaagggcc
     1081 attatcgtag agcatctttt cgaaatgggg aattcgccaa tattgatcgg tactataccg
     1141 gaagaaacct ccccctaatt ggtcgtaaat accgcccttt gccattttct ccagcgtatg
     1201 tttcacaatt ctcagtgccc ctgcatcgct atcataaaaa tagcaatgca ggcaaaatag
     1261 taattctgct ggatgaagaa atttaggtgc ctgaccaaaa ccaccgtact cccgatcaaa
     1321 attctcatct aattgagtca atgctcttaa gatcaattgt cgagaaatga ctggaatctt
     1381 ggcccctgtg gtaggtaatg tatctgcgag taatttcagc agcgccgtat tttgtttctc
     1441 aatatcatcg gttcgagttc gatacgtttc tgcaatcttg ggtaacaatt ccaaaaaacc
     1501 gggcatacca tgacgtgata ctttaggaaa ataagtgcct ccaaaaaaag gcttttgctc
     1561 tggcgtcaga aacatcgtta aaggccatcc accatggcgc tgattgatca tgtgatgcgc
     1621 cgtttgatag atttgatcaa tatcaggtcg ttcttcccta tcaactttga tattgacgaa
     1681 atattgattc attgccgcag cgacttcagc atcttgaaat gattcattgg ccatcacatg
     1741 gcaccagtga cacgctgaat agccaatcga aagtagaatt ggcttattca attttttagc
     1801 gagttctagc gcttctttat tccatggatg ccattcaact gggttatcca catgttgcaa
     1861 aagatagggg cttgtttcat gtatcaggtg atttggcatg tcagtctgtg attgcgccac
     1921 ggctagcggt cgacacaagt tttgcgtatt tcgctaacac accccgatga taccgggcct
     1981 taggaggttg ccaagctgca cgcctttttt caagctcatc ttcagatata ttgagttgca
     2041 ataatcgttg cgtcgcatca atcgtaatgg agtctccctc tctaactaag gcaattaccc
     2101 cgcctacaaa tgcttcaggg gaaacgtggc caacaaccat tccataggtt ccccctgaaa
     2161 atcgaccgtc tgtaattaga ccaactgaat cacccagccc ttctccaata agtgcagagg
     2221 ttggggacag catttcccgc atacctggcc cacctttagg cccttcataa cgaatcacta
     2281 ccacgtcacc cgcatgaatt tctcttgcca gaattgcaga catacacgat tcctcagaat
     2341 cgaaaaccct tgccggccct gtaattttag gattcttaac accggatatt ttggcaaccg
     2401 caccttctgg tgacaaattt cctttgagaa ttgcaaggtg accttgttca taaagtggat
     2461 caccccattg acgaataatt ttttgatttt gtggaggctg atccggaaca ttatgaagta
     2521 cttgctctat tgtctcaccg ctgattgtca agcaatcacc atgaagcaag ccattgttta
     2581 gaagcatctt catcacttgc gggattccac ccgctttatg aagatcagcc gttacatatt
     2641 gtccagaagg tttaagatcg catattacgg gaactcgtgc acgaatcact tcgaaatcat
     2701 tgatatcaag ctcaacttct gctgcatggg caatagctag taaatgcaat actgcattcg
     2761 ttgaaccacc caccgccatc acaacagaaa tcgcattttc aagtgatttt ctcgtaatta
     2821 aat
//
LOCUS       DAOQLJ010000239         2781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_21207_length_2781_cov_5.292737, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000239 DAOQLJ010000000
VERSION     DAOQLJ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21207_length_2781_cov_5.292737"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PK522_11485"
     CDS             complement(<1..345)
                     /locus_tag="PK522_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110690.1"
                     /note="catalyzes the formation of
                     4-amino-2-methyl-5-phosphomethylpyrimidine from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole and
                     S-adenosyl-L-methionine in thiamine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNC42397.1"
                     /translation="MNTIISKLETFTSETAQVDQAAVKPLPNSRKIYISGSSSDIRVP
                     MREISQTDTVTQTGAEKNPSIYVYDTSGPYTDPDIAIDIRNGLYPLREKWIEARGDTE
                     LLSGLTSAYSRER"
     regulatory      complement(380..484)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            548..1219
                     /locus_tag="PK522_11490"
     CDS             548..1219
                     /locus_tag="PK522_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HNC42398.1"
                     /translation="MKHNTHIDIEKTRFNMVEQQIRTWNVLDDTILNLLYKIHREEFV
                     PAAYRALAFTDMEIPLEHGAVMLTPKMEARIVQELHIKKTDKILEVGSGSGYMTALLA
                     ELGAHVHSVEIVPTLCAMAKINLQTHDITNVTIEQGDASQGWPNHAPYDVIVLTASTP
                     VLPEAFQKSLNPGGRLFAIVGEDPVMEAILITRTASDTYQTTHLFETCTAPLQNAQQP
                     PRFTF"
     gene            1289..2605
                     /locus_tag="PK522_11495"
     CDS             1289..2605
                     /locus_tag="PK522_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HNC42399.1"
                     /translation="MKKSLFLVAWVISITLSLPLHAANLMEIYREALEHDAQYGSARA
                     AYIAAQEKLPQGRAGLLPTINATGTAQNQYIDTNLGSEVLIRNRGVVIAATQPLFRIE
                     NFIIYEQSKNQVAQADARFVLAAQDLIIRVAQAYFNALIAQSNLEVVETQKKAIQQQL
                     EQAKRNFEVGVSTIVDTHEAQARYDLTLSQEIAARNALEISKRTLQGIINRFPDSLVR
                     ISIDKIITDKLDLAHDNMEEWVNIAEQKNFSLRVQQIAFDLAEQEISKAKAGHYPTLD
                     LVAQYTDQQGVGGAITGRGIDLTSKSVGLQLNVPIFQGFSVQSRVREALANRDRARMD
                     LENIRRTIGLQVRQQYLNVTNGIAQVKALKQALTSSKSQLDSTLLGQEVGVRTEVDVL
                     NAQQQLFSARRDLVQAYYNYLMARLRLKAEVGELDEEVLQEINALL"
BASE COUNT          864 a          579 c          588 g          750 t
ORIGIN      
        1 tcgttctcga ctataagctg aggtaagccc tgaaagtaat tctgtatcgc cgcgggcttc
       61 tatccatttt tcacgcaggg gataaaggcc attgcgaata tcgattgcaa tatcaggatc
      121 ggtataagga ccagatgtgt catacacata aatcgaggga ttcttttcag cgcctgtttg
      181 agtcactgta tccgtttgtg aaatttcacg cataggtacc cgaatatctg aacttgaccc
      241 tgatatatag atcttacgag aattgggtaa cggtttaaca gcagcctggt cgacttgcgc
      301 cgtttcgcta gtaaatgttt ctaactttga aatgatggta ttcatggtat gctccatatc
      361 aattcgcaag gagcagaagg agtaatcgct tcagcttccc tacggcggca ttatccgcat
      421 caggtattaa gggactttct caccgacaat ttaataatca aaatgcggac ccctagctta
      481 agctgtgaaa ctaatggaaa tatgaaataa ttgcaagtcc gattcttttt aatcatcaat
      541 gctaactatg aaacataata cccatatcga tatcgaaaaa acccgattca acatggttga
      601 acaacaaatt cgtacctgga atgttttgga cgataccatt cttaatttgc tctataaaat
      661 ccatcgtgaa gaatttgttc cagcagccta tcgtgcgctt gcttttacgg atatggagat
      721 cccacttgag catggggctg taatgctaac gcctaaaatg gaagctcgca tcgttcaaga
      781 attacatatc aaaaaaactg acaaaattct tgaagtgggc agtgggagtg ggtacatgac
      841 cgcgttactc gcagaattag gtgcacatgt tcatagtgtc gaaattgtac cgactctatg
      901 cgccatggcg aaaattaatc tacaaacaca tgacattact aatgtcacga tcgaacaagg
      961 agacgcttca caaggttggc ctaatcatgc accctatgat gtgattgtgt taactgcttc
     1021 aacccctgtg ctaccggaag ctttccaaaa aagcttgaat cccggtggaa gattgtttgc
     1081 aattgtaggc gaagatcctg ttatggaagc aatattgatt actcgcacgg catcagatac
     1141 ttaccaaaca acccatttat ttgaaacctg tacagcgcca ctgcagaatg cccagcaacc
     1201 acctcgattt actttttagt cattcacttt taatgtcatc aacagcaatc gatagccctc
     1261 catgattgtt accttaacta actaattaat gaaaaaaagc ctttttttag ttgcatgggt
     1321 aatcagtatc acgttatcac tgcctttaca tgcggccaat ctaatggaga tttatcggga
     1381 agcactcgaa catgatgctc aatatggatc agcgcgagca gcttatatcg ctgcccaaga
     1441 aaagttacct cagggaagag ctggtttatt gccaacgatc aacgcgactg gaactgcaca
     1501 aaatcaatat atcgatacca atcttggatc agaagtatta atcagaaatc gaggtgtagt
     1561 cattgctgca actcaacctt tgtttcgaat tgaaaatttt attatctatg agcaatcaaa
     1621 aaatcaagta gctcaggctg acgcaagatt tgtattagct gcacaagatc tgatcatccg
     1681 tgtcgctcaa gcttatttca atgccttgat tgcacaatcg aacttagagg tagttgagac
     1741 acaaaaaaaa gcaatacagc aacaactaga acaagcaaaa cgcaattttg aagtaggtgt
     1801 atccactatt gttgataccc atgaagcgca agctcgctat gatttaacac tttctcaaga
     1861 aattgcggca agaaatgccc tagaaattag caagcgtact ttgcaaggta tcattaatcg
     1921 attcccggac agcttggtaa gaatcagcat tgataagatc attacggata aattggattt
     1981 agcccatgac aatatggaag aatgggtcaa tattgcagaa caaaagaatt tttctcttag
     2041 agttcagcaa attgcctttg atttagctga gcaagaaatt agtaaagcaa aagcagggca
     2101 ctatcccact ttggatctag ttgcacaata cactgaccaa caaggcgttg gtggcgctat
     2161 tacgggtcga ggcattgatc tcacttctaa atcagttggc ctgcaactga atgtgccaat
     2221 ttttcaagga ttctcggttc aatcacgagt tcgtgaagcc ctggctaacc gtgatcgagc
     2281 acgaatggat ttagagaata ttcgccgcac aattgggtta caggtacgtc aacaatacct
     2341 gaatgtcact aacggaatcg ctcaagtcaa agctttaaag caggcgctga cctctagcaa
     2401 aagtcagtta gactctactt tacttggcca agaagtgggt gttagaaccg aggtcgatgt
     2461 attaaatgcc cagcagcaat tattctcagc gcgacgcgat cttgttcaag cctactataa
     2521 ttatctcatg gcacgactac ggctcaaagc agaagtcggt gaattagatg aagaagtttt
     2581 gcaagaaatt aatgcgttgc tctaaggaaa tggttttcta cccatcgaat agaaagaatt
     2641 gatagaatac aaactgaagc tgactagaca gtcgctgcct gtatttgcag ggagaggaaa
     2701 gtccgggctc caaagagcgg gatgtcggct aacggccgac cactgtgaag tgaggaatag
     2761 ggccacagag acgagcgtat t
//
LOCUS       DAOQLJ010000240         2734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_21881_length_2734_cov_5.539007, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000240 DAOQLJ010000000
VERSION     DAOQLJ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21881_length_2734_cov_5.539007"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            390..1001
                     /locus_tag="PK522_11500"
     CDS             390..1001
                     /locus_tag="PK522_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNC42400.1"
                     /translation="MRNYKQIVFDIETVPLPNADEIIASSKSLRRLKDPDKIEAKKIE
                     LLEKAALSPMTGSIALIGYYDGEETKVLDARDYGEKRLLEEFWEISCDKLLGWNCLMF
                     DIPFIIKRSWCYGVHIPVWAQTSIGRKQSVIDLAQEFAMSRDYESLEDVMRFFGMKPK
                     NSDNSAKFYELYNNYPIAAIEHCKVDVEATWKIGEKMGFITPF"
     gene            1589..2467
                     /locus_tag="PK522_11505"
     CDS             1589..2467
                     /locus_tag="PK522_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC42401.1"
                     /translation="MELVRATRKKSKLRLNLSAVSGAGKTMSALLMAKGLVGDWSKIA
                     VIDTESGSASLYSHLGGFFTLDLKPPFSPERYIEAINKCLDAEIECIILDSATHEWNG
                     EGGCLQINQSIANSQFKGNTWAAWSVVNPRHDAFVSKILHSPAHFITCTRSKDETVMG
                     DDRKVKKVGIKDIQREGWEYELTVSLHIDRDSHKASAVGGKDRTGRFEVMDPFIITEK
                     TGEMIRDWCNEGVDMDVTERDIIIGKLESALSMDELKSIWLSHSQTISRLSSKDQAMI
                     DSTKSMMKTKLSQSNE"
     gene            2486..>2734
                     /locus_tag="PK522_11510"
     CDS             2486..>2734
                     /locus_tag="PK522_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42402.1"
                     /translation="MNWYDVGLTELTCPYCDAEVAATVTGKVNCEHCGKDFVIEQLPP
                     KFNCLKVRCDEVGKEHDFQFVRNVYTKDYKEIIGSIHVC"
BASE COUNT          923 a          436 c          618 g          757 t
ORIGIN      
        1 agggggaggc aaggtgcgat cccctcatac aaaaacatgg gaggacatcg gatataccga
       61 acacaatatg gtagaagtca tagtagatag tgaaggcaac ttgtttaagg tagattattc
      121 ggagggatta tgacaatact tgaacttgtt gaggaactaa aaaaacgagg caagtcattg
      181 acgagattta ctatctatca acatattaaa tccggcaatt taaaggaggg acaacattat
      241 tttatacgta aaatgcacta tgtgatgctt gaatcatcca ttaataagtt aatgaacatg
      301 agagttggaa ggagaattgg aagcaaagat tctaaaccaa gatctaagaa aaaacttgac
      361 aattgaataa tttttaacta tattgggtta tgagaaatta caaacaaata gtatttgata
      421 ttgaaacggt accactacct aatgccgacg aaataatcgc aagtagtaag tctcttcgta
      481 ggcttaagga tcctgataaa atagaagcta aaaaaataga gcttcttgaa aaagccgcct
      541 taagtccaat gactggctca atagctttga ttgggtatta tgacggtgaa gaaaccaaag
      601 ttttggatgc aagagattac ggtgaaaaac gacttttaga agagttttgg gaaataagtt
      661 gtgataagtt attgggttgg aattgtttga tgtttgatat tcctttcatc ataaagagat
      721 cttggtgtta cggcgttcat ataccggtat gggctcaaac ctctattggt agaaaacaat
      781 ctgtaattga tttggcacaa gagtttgcta tgagcaggga ttacgaatct cttgaagatg
      841 ttatgcgatt tttcggtatg aaaccaaaaa actccgataa tagtgcaaag ttctacgagt
      901 tatataataa ctatcctatt gcggctattg agcactgcaa agtagatgtt gaagctactt
      961 ggaaaatagg agaaaaaatg gggtttatta ctccattctg atgttatttg aaaattataa
     1021 ggtgctgtgg cggacagtga gccgcgaggt gaagcaagaa agttcgattc tttcttttgt
     1081 cttggtagca tagaccgcga gtctgcggaa agagcgagtg tggttcaaat ccacaataga
     1141 cctgcaagtc aaaccttgcc agcacctaaa ttataaagcg atcacgtggc ggaaggagtg
     1201 tggagtccat gctcgataga cgcttgcgtg atcgcttgat tttaaaatta aggtttcagc
     1261 cttaaaaaca aagtaagaat gtaaggttat agactgaaac tatattatag caatacaaat
     1321 ttaattagtg aatcgattta tgtttaaatt aagccagatg cagtagcggt ataataaact
     1381 atgtgtaggg cgaaaatccg agatagtgta aagacctgca aaagacggga aaccggttga
     1441 agctattgcg tttggctaat attataaatc atgaaagtgt ttataattat tttttataaa
     1501 ccgcacaaat aaacaacaaa acaaacctaa tggtttgata aacagttata ggccgagtgc
     1561 ggcggcctat tttatttaag gagataaaat ggaacttgta cgtgcaacgc gaaagaaaag
     1621 taaactaagg ttaaatcttt ccgcagtatc gggagctggt aaaacaatga gcgctctttt
     1681 aatggctaaa ggacttgtcg gagactggtc aaagatagct gtaatagata cagaatccgg
     1741 ttccgcctca ctttattccc atcttggtgg tttctttact cttgatctca agcctccatt
     1801 ttctccagaa cgatatattg aagctataaa caagtgtctt gatgcggaaa ttgaatgtat
     1861 aatccttgac tcagctacac acgaatggaa tggagagggt gggtgtttgc aaatcaatca
     1921 atctatagca aattcgcaat ttaagggtaa tacatgggcg gcatggagcg ttgtaaatcc
     1981 taggcacgat gcttttgtgt caaaaatatt acattcaccc gctcatttta ttacgtgcac
     2041 acgctcaaaa gacgagacgg ttatgggtga tgatcgcaaa gtgaagaaag tcggtataaa
     2101 agacatacaa cgtgaaggat gggaatatga attaacagta agtcttcata ttgaccggga
     2161 cagccataaa gcctctgccg ttggtggaaa agaccgtacc gggcgttttg aggtcatgga
     2221 tccattcatt ataaccgaaa agacaggaga aatgatacga gactggtgta atgagggtgt
     2281 tgatatggat gtgacagaac gtgatattat tattggtaag cttgaatccg cactctcaat
     2341 ggacgaactc aagtctattt ggttatcgca ttctcagaca atatcgagat tgagtagtaa
     2401 agatcaggcc atgatagata gcactaaatc aatgatgaaa accaaactat cacaatctaa
     2461 tgaataattc aaacgccgag acactatgaa ctggtatgat gttggcttaa ccgaactaac
     2521 ctgcccgtat tgcgacgccg aagttgccgc tacagtgacg ggcaaagtaa actgcgaaca
     2581 ttgcggcaaa gactttgtga ttgagcaatt accgcctaag tttaactgcc tgaaagtgcg
     2641 atgcgatgag gttgggaaag agcatgattt tcaatttgtc cggaatgttt atactaaaga
     2701 ttataaagaa attatcggct caattcatgt atgc
//
LOCUS       DAOQLJ010000241         2733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_21900_length_2733_cov_3.880881, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000241 DAOQLJ010000000
VERSION     DAOQLJ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21900_length_2733_cov_3.880881"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..1367)
                     /locus_tag="PK522_11515"
     CDS             complement(213..1367)
                     /locus_tag="PK522_11515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC42403.1"
                     /translation="MEMQQQIELTALLLAENWQAVKNFLDSTHPGMLDAQGNFAKGFL
                     LAFGPAHQRNLTEAFNYLEIANRLAPANILYLNTLSEAYLQAKRPAMALRMATQAAQL
                     APGNLFTAIALGRAAWFCNEKEFALSAFEEAYRLTPSSQPSLKEHLYAITFSSAPFWR
                     EPCQGKRIVLVRLEPRHRDFLMSCRRNIQFQHHYHLFQDSSNEAIERDLRLANRPPLE
                     TKRISWVVEKDKKPIGLAELVDLNLNNARAEILVGFPEKQPFGISLEATLLIMEFAFS
                     KIGLYKLISYVYGDNSGSQRNTLHLGFQQEGLLRSHVIDPASKKRLDLYNNGCLSTEF
                     FQNKNLMRLANRLLGRIPEPGNTGLSRKISDQGDISSLIIKLPLIIAKDK"
     gene            complement(1530..2387)
                     /locus_tag="PK522_11520"
     CDS             complement(1530..2387)
                     /locus_tag="PK522_11520"
                     /inference="COORDINATES: protein motif:HMM:NF025314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HNC42404.1"
                     /translation="MTTNNEQIKLTDTSLSFSTADSGSGSKTGSGSKTDSSQNLQGGV
                     SGNVSFNGGNNIDTVSYVIPYSEVTAITQSKDGSWAISYNMSSSTGSGSSSGGKSGGS
                     GSSGGKSGGGSSSGGKSGSGGTTTATDTLHSIERLNFSDGVNVALDINSPDQVAGAAL
                     ALLYAGFHSLPDATTFGHWIAKADLLHDSQAFDNSKVENLAQEMLAEYAPSGISNSAL
                     INILYTNVVGHTPNSSDLNYFSQLLDKGTYSQAGLFALAAESNLNTEQYTTLIGNGLQ
                     YVPESGKVG"
BASE COUNT          756 a          585 c          581 g          811 t
ORIGIN      
        1 gtaccagcca cctttgtcag ttcgatattc gaccgagtgc ggccatgcgt actgtattca
       61 ccgctgctgc cggtatgttt ccaatgaacc acgccgagat cttccgggcc gcctttctat
      121 aagccgtaca ccgctaccaa tccattgttg gcatccacgc cgctgtatgc ggtggcggtt
      181 gcaccgttgg cgtggatatg cgtccaatcc gatcatttgt cttttgctat gattagtgga
      241 agcttgatta tcaagctaga gatatcccct tgatctgata tctttctgct taatccggtg
      301 ttacctggtt ccggaattct ccccaataat ctgtttgcaa gcctcatgag gtttttgttt
      361 tggaaaaact cagtagataa gcaaccatta ttataaaggt cgagccgttt cttagatgca
      421 ggatcaatga catgcgacct taacaaccct tcttgttgaa agccaaggtg caatgtgttg
      481 cgctgacttc cggaattatc gccataaaca tagcttatta gcttatagag gccaattttt
      541 gaaaacgcga attccataat aagtagagtg gcttcaaggc tgatgccaaa aggctgtttc
      601 tcaggaaaac caactagaat ctctgctctc gcgttattta aatttagatc aaccaattcc
      661 gccagtccaa taggtttctt atctttttct accacccaac tgattcgttt ggtttccaat
      721 gggggacgat tagctaatcg taaatcccgt tcgatagctt cattggaact atcctgaaac
      781 aaatgataat gatgttgaaa ttggatatta cgccggcatg acattaaaaa atcgcgatgt
      841 cttggctcca atctaactag cacaatcctt tttccttgac atggttctct ccagaaaggt
      901 gccgagctga aagtaatcgc ataaaggtgt tctttaaggg aaggctgact tgatggagtt
      961 aaacgataag cttcttcaaa agcgctaaga gcgaattctt tctcgttaca gaaccatgca
     1021 gctcgtccca aagcaatggc ggtaaataga ttgccgggtg ctaactgcgc tgcctgtgtt
     1081 gccatacgta aggccatagc agggcgtttt gcttgaagat aagcttcaga aagcgtattc
     1141 aaatacagaa tattggcagg cgcaagccgg tttgctatct caagataatt aaatgcctca
     1201 gtaagatttc tttgatgtgc gggtccaaaa gctaaaagaa atcctttcgc gaaattacct
     1261 tgtgcgtcaa gcatgccagg gtgggtcgaa tccaagaaat tcttaactgc ttgccagttt
     1321 tccgcaagaa ggagagcggt aagttcaatt tgctgctgca tttccatgat taatggcttt
     1381 ttaatggaat ctgtcaagaa tcatcaggat aaatgcggga ggctgttact taagagtata
     1441 cctcagtctt ttcagaagga ctgaggtcat actgcaaggg cagattcaaa gttagaagag
     1501 cagaaaaata ttgaatttat attttattat caaccaactt ttcctgattc aggtacatat
     1561 tgtagtccat tgccaattag tgttgtgtat tgctctgtgt taagattgct ttcagcggct
     1621 aacgcaaaca gtcccgcttg agaataagtg cctttatcga gtaattgcga gaaataatta
     1681 agatcgctgg aattaggggt atgtcccact acgttggtat ataaaatgtt gatgagagca
     1741 gagttactga taccacttgg tgcatattca gcaagcattt cctgagccag attctcaact
     1801 tttgagttat caaaggcttg actgtcatga agaagatccg cctttgctat ccaatgacca
     1861 aaggtagtcg catccggaag ggaatggaag cccgcatata gtagagcaag cgcagcacca
     1921 gcaacttgat ctggagaatt gatatcgagt gcaacattaa caccatcaga aaagttcagc
     1981 cgctcaatac tatggagagt atctgttgca gtagttgtgc caccactgcc actttttccg
     2041 ccgctactgc taccaccgcc actttttcca ccgctactgc cactaccacc gcttttcccg
     2101 ccgctgctac tgccactgcc tgtagaggaa ctcatattat agctaattgc ccaagatcca
     2161 tctttgctct gagtgatagc agtaacctcg ctatatggaa tcacgtatga aacagtatcg
     2221 atattatttc cgccattaaa actgacattt ccagatacac caccttgaag attctgagat
     2281 gagtccgtct tacttccaga acctgtcttg ctaccgctgc cactatcggc agtactaaag
     2341 ctaagcgatg tgtcagttag tttgatttgt tcattattag tcgtcatatc gattcctttg
     2401 gttttggtac tagcaatgat tttattggtg ctaaaattat ttatgttttc taagtgatta
     2461 cttgatcatt ttatcctttt tctttttctg tacggaaaaa gtttactggc ggactatgaa
     2521 tcatgcaagc tacttggcag atttcgatac aagatactga gtaatgataa aaatatgctg
     2581 ctagtatttt tgcgatcata ttagcgagaa tatctaatag gagaagtaaa acgcatgaaa
     2641 atccacgaat accaagccaa ggaaatttta cgcaaatacg gtgtggcgac accacaaggt
     2701 atcgcgtgtt tcagtgtcga cgaggcggtg aat
//
LOCUS       DAOQLJ010000242         2712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_22173_length_2712_cov_5.568310, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000242 DAOQLJ010000000
VERSION     DAOQLJ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22173_length_2712_cov_5.568310"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PK522_11525"
     CDS             complement(<1..233)
                     /locus_tag="PK522_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42405.1"
                     /translation="MHDIDRTQLESNPALENYEQEQYEYGEFGEYGETEWSGEAGIFN
                     ESQAMELAAELLEVSSEEELDRFLGGLIKKAGQV"
     gene            complement(273..965)
                     /locus_tag="PK522_11530"
     CDS             complement(273..965)
                     /locus_tag="PK522_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42406.1"
                     /translation="MYQSENTFEFNPEMSGFETEQYEGEQYEGEQFEQYEYGEAEWGE
                     VFNESEVMELAAELLEVTNEAELDRFLGNLIKKAGSALGKVVKSPIGQAVGGVLKGVA
                     KKALPIAGGALGAYFGGPLGAKIGSGLASAAGSALGLELETLNQEDREFEGGKQFVRF
                     AANTVKNAVSAPPAANPRSTAQMAANKAAQVYVPNLITSGPALTQPSAMPSKGRGGRW
                     FRRGRNIVIVNC"
     gene            complement(1495..2259)
                     /locus_tag="PK522_11535"
     CDS             complement(1495..2259)
                     /locus_tag="PK522_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNC42407.1"
                     /translation="MFKKINKYVLLTVAGLILSNPVAADRTVDDFQVWGNVTALGNFG
                     FINPEKPELKKFRWWMEGQGRFGNDATQFTQSLVRPGVGYAVTDKMVVWVGYAWAPTA
                     EPLTLDTGHPFNEHRIWEQVTWADNFWFGRLSLRSRFEQRFFDQNAPLPGHNDVAHRF
                     RQLVKLAVPLTAINPDLSFIVQSELFIAMTTIDNNAGFISRGFDQNRGFVGLGYKINQ
                     YANVELGYMNQFINRPHSARPDQMMHNLVANLFLNF"
BASE COUNT          787 a          639 c          494 g          792 t
ORIGIN      
        1 acttggcccg cttttttaat gagaccgcct aagaatcgat ccaactcttc ttcactgcta
       61 acttccaata actcagctgc caattccatg gcttgagatt cgttgaaaat accggcttcg
      121 ccactccatt cggtttcacc atattcaccg aattctccat attcgtattg ctcctgttca
      181 taattttcca atgcagggtt gctttccagt tgggttcgat caatatcgtg catgatcatt
      241 ctcctcacaa ataggtttat agaagttgct tcttagcagt tgacaatgac gatattgcga
      301 ccacggcgga accatcgacc tccacgccct ttacttggca tggctgatgg ctgggttaat
      361 gctggccctg aagtgattaa attgggaaca taaacttggg cagctttatt tgcagccatt
      421 tgtgcagtgc tgcgtggatt agcagcaggc ggtgctgata ccgcattctt cacggtattc
      481 gcggcgaaac gcacaaattg ttttccccct tcaaattcgc gatcttcctg attaagtgtt
      541 tctagttcca atcccagcgc actgcctgct gcggaggcca aaccactccc aatttttgca
      601 cccagcggcc caccaaaata agcacccagt gctccaccag caatcggcaa tgctttcttg
      661 gctactcctt tcagtactcc tccgacggct tgtccaatag gagatttcac tactttacct
      721 aatgcactac cggctttctt aatcaggttt cccaagaaac ggtcgagctc agcctcattc
      781 gttacttcca gaagctcagc cgcaagttcc atcacttcac tttcgttgaa tacttccccc
      841 cattccgctt ccccatattc atattgctca aactgctcgc cttcatattg ttcaccttca
      901 tattgttcgg tttcaaaacc actcatttcc ggattgaatt caaaagtatt ttcactctga
      961 tacatggtat tggccctccg ctaattttta attcgaatgc ttgagatttg agataaatga
     1021 gcgttgtgcg ctcctgtcag gatgatcggt ctgatttcga cttcctcccc tttagctata
     1081 tacacatagc gtgccaaaaa aatatctcaa cctaattcca ctcaaagaat agaggtaatc
     1141 aactgaaagt taaggataaa taatgctaga gacccaatag aatgggcaaa aatgaagctt
     1201 agcgctttgc gaagattgct tcgttccagg aaacaaatcg gcgatcgagt gcgaaaatca
     1261 tttcgccttg caaaaattga agttaattga ccagcttact aacaaagatt tcagttcgac
     1321 tgcaaaatca caatcaaaag agagatattg agccactgta tgatatttac acgattattc
     1381 agtgacaatc tgactatcag caaaaaaaat aagccgattg atccatacta gctaaaatca
     1441 atcggctact taaaacgtta tacgatatgc gagaccccat atcgtaggaa gaacctagaa
     1501 attcaaaaac agatttgcta ctaagttgtg catcatttgg tctgggcgtg cgctatgcgg
     1561 tctgttgatg aattggttca tataacccaa ctcaacattg gcatattgat tgattttata
     1621 acccaatccg acaaaccccc ggttctgatc gaaaccgcgg gagataaagc cagcattatt
     1681 atcaatagtg gtcatcgcaa tgaacaactc actttgaaca ataaagctta aatccggatt
     1741 aattgcggtc aatggaactg ctagtttaac caattggcgg aaacgatgag cgacatcatt
     1801 atggcctgga agcggcgcat tttgatcaaa gaatcgttgc tcaaagcggc tgcgtagaga
     1861 caaccgacca aaccagaaat tatcagccca agttacttgc tcccaaatgc ggtgttcatt
     1921 gaagggatgt cctgtatcta atgtcaatgg ctcagccgtt ggggcccatg cataaccgac
     1981 ccacacaacc attttatcgg taactgcata accaaccccg ggacgaacca gtgattgcgt
     2041 aaattgagta gcgtcattac caaaacgccc ttgtccttcc atccaccatc tgaatttttt
     2101 caattcaggc ttttctggat tgatgaaacc aaaattaccc agcgcggtaa cgtttcccca
     2161 cacttggaaa tcatccacgg ttctatccgc agcaaccgga ttactcagaa tcagaccagc
     2221 cactgtcagt aaaacatatt tatttatttt tttgaacatg taaactcctt gcttaaataa
     2281 tgtgatttta tgaagctatt tttactaata ttctttatat aagttaaaca gcataagctc
     2341 acataattaa cgattgtttg ttgattgtag ttagttactc ttacctttat aaaatttaca
     2401 ccctattcct ttagtaaata ggaattttct taatatatta gctacatgcc ctgaacacgc
     2461 agtaacaagg taactgtcga gaaagtaaca cagtagctgt caagaaaatg taaagaatta
     2521 cccctgaagt aaacagtgct attgtcatta aactttcact gaaatagctg aagttaagta
     2581 cttatcgcac ttataaatat tttttgaagc agatcgaacg agccaaaaac gtagcgtttt
     2641 ttcattcacg ctttctaaat tcgcagatta tcgatgctaa ttggagcgcc ccctttgggt
     2701 gaacatatta tg
//
LOCUS       DAOQLJ010000243         2712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_22178_length_2712_cov_3.823861, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000243 DAOQLJ010000000
VERSION     DAOQLJ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22178_length_2712_cov_3.823861"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PK522_11540"
     CDS             <1..260
                     /locus_tag="PK522_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HNC42408.1"
                     /translation="YAIGVAKPVSLMVDTFGTGKISDEALVKLIEQHFDLRPRAIIHQ
                     LDLLRPIYGKTAAYGHFGREEDGFTWEKTDMATQLRSAAGI"
     regulatory      315..399
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            417..1844
                     /gene="ahcY"
                     /locus_tag="PK522_11545"
     CDS             417..1844
                     /gene="ahcY"
                     /locus_tag="PK522_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020162970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNC42409.1"
                     /translation="MNAVTNPSGLSNFNDYKIADATLAEWGRKEIAIAETEMPGLMAV
                     REEFANKKPLAGARIAGSLHMTIQTAVLIETLVELGAEVRWASCNIFSTQDHAAAAIA
                     ANNIPVFAYKGETLSDYWDYAHRIFEWPNNTHANMILDDGGDATLLLILGSKAEKDAS
                     ILNNPTNEEEQALYSAIKSKLTQYPNWYSTRLAKIHGVTEETTTGVHRLYEMQKKNEL
                     PFPAFNVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKIAVVCGYGDVGKGCAQS
                     LRGLGATVWITEIDPICALQASMEGYRVVTMDDACDKADIFVTATGNIRVIGHDHMIK
                     MKNQAIVCNIGHFDSEIDIASIHQYSWENIKPQVDHVIFPTGRRIIVLAQGRLVNLGC
                     ATGHPSFVMSSSFTNQVLAQMELWQNGSNYQHEVYVLPKHLDEKVARLHLSKLGATLT
                     ELTDEQSNYLNLSKTGPYKPENYRY"
     gene            1963..>2712
                     /gene="metF"
                     /locus_tag="PK522_11550"
     CDS             1963..>2712
                     /gene="metF"
                     /locus_tag="PK522_11550"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNC42410.1"
                     /translation="MQSQKKFPATFSFELFPPQTPEGIEKLRVARSQLAQFNPKFFSV
                     TYGAGGSTRDRTIKTVLEIQAEGRTVAPHLSCIGSTRENIYALLKKYQENGIHHLVAL
                     RGDLPSGMAQAGEFRYANELVTFIRQEFGDLFRIEVAAYPEYHPQSRSAQDDFKNFIK
                     KIEAGADSAITQYFYNADAYFHFIDQCEAANLRIPVVPGIMPINKFTQLVRFSDTCGA
                     EIPRWIRKKLEGYGDDSASITAFGLDVVTELC"
BASE COUNT          810 a          544 c          563 g          795 t
ORIGIN      
        1 cctacgcgat tggcgttgca aaaccggttt cgctcatggt cgatactttt ggtaccggta
       61 aaatatcgga tgaagcatta gtgaaattga tcgagcaaca ttttgattta cgtccgagag
      121 cgattattca ccaactcgat ttattgcgcc ctatttacgg taaaactgct gcatatggcc
      181 attttggtcg tgaagaagat ggttttactt gggaaaaaac cgatatggcg acacaacttc
      241 gtagcgctgc gggtatctag aatagaattt tcatcattta tcaatcaatg ataataatct
      301 aagcttttat cttattaact gaggagcgct gcagcggttg attaaccccg ttaggctcag
      361 ttgataagat taatatcgtt tcaacggcgc tcgttcattc ttcaggagac ttgactatga
      421 acgcagtgac taacccttca ggcctaagta attttaatga ttataaaata gccgatgcta
      481 cattggccga atggggcaga aaagaaatcg caattgctga aactgaaatg cctggcctta
      541 tggcagtgcg tgaagaattt gcaaataaaa aacctttggc aggagcacgg attgctggct
      601 cgctgcatat gaccattcaa acggcggtac tcattgaaac attggttgaa cttggtgccg
      661 aagttcgctg ggcttcttgc aatatctttt ctacccaaga tcacgctgca gcagctattg
      721 cagcaaacaa tattcctgtg tttgcctaca aaggcgaaac actcagcgat tactgggatt
      781 atgcgcatcg tatttttgaa tggcccaata atacccatgc aaacatgatc ttagacgatg
      841 ggggtgatgc aacattactt ctgatccttg gcagtaaagc tgagaaagat gcttcgatac
      901 tcaacaatcc tactaatgaa gaagagcaag cactctattc agcaattaaa agtaaactta
      961 ctcagtaccc taactggtac tcgactcgtt tggctaagat tcatggcgtt accgaggaaa
     1021 caactaccgg tgtgcatcgt ttatacgaaa tgcagaaaaa aaatgaactc cctttccctg
     1081 ctttcaatgt aaatgattcg gtaaccaaat caaaatttga taatttatat ggttgtagag
     1141 aatctcttgt cgatggcatt aaacgcgcaa ccgatgtaat gattgctggg aaaattgctg
     1201 ttgtatgcgg atatggtgac gttgggaaag gatgtgctca atcattgcgc ggtctcggtg
     1261 caacggtatg gattacggaa atcgatccaa tctgcgcact acaagcttcc atggaaggat
     1321 atcgcgtggt aaccatggat gatgcttgcg acaaagcgga tatttttgta accgcaactg
     1381 gtaatattcg ggtcattggt catgaccaca tgattaagat gaaaaatcag gcaattgtct
     1441 gcaatattgg tcattttgat tcagaaattg atattgcttc aatacaccaa tactcatggg
     1501 aaaatattaa acctcaagtc gatcatgtta ttttccctac gggtcgaaga attatcgtgt
     1561 tagctcaagg taggcttgtc aatcttggtt gtgcaactgg acacccttca tttgtaatgt
     1621 cgagttcttt cactaatcag gtattggctc aaatggaact ttggcaaaat ggcagtaact
     1681 accagcatga agtttatgtt ttaccgaaac atttagatga aaaagtagct cgacttcatt
     1741 tatctaaatt gggcgcaaca ctaactgaat tgaccgatga gcagtctaat tatctgaatc
     1801 tgagtaaaac aggtccttat aagcctgaaa actatcgtta ctaattttct atttaatcga
     1861 actaaagggt cagcggattc tcaattatgc tatgctgacc ctttaataca ttttcataaa
     1921 tgactctgtt taggagtctt acctcaatca ttgttaggcg agatgcaatc acaaaaaaaa
     1981 tttcctgcta catttagttt tgaattattt cctcctcaaa cccctgaagg aatcgagaaa
     2041 cttcgtgtag cacgttcgca actcgcgcaa ttcaatccaa aatttttctc cgttacctat
     2101 ggtgcagggg gatcaacccg tgatcgtacg atcaaaaccg ttcttgaaat ccaggcagaa
     2161 ggccgtacgg ttgctcccca tttatcttgt attggctcaa ctcgcgaaaa tatttatgca
     2221 ttacttaaga aatatcaaga aaacggtatc catcaccttg tagctttacg aggcgatctg
     2281 ccatcgggaa tggcacaagc aggtgagttc cgatacgcaa atgaacttgt cacgtttatt
     2341 cgccaagaat ttggtgatct tttcaggata gaagttgcgg cttacccaga atatcatcct
     2401 caatcgcgct cagcacaaga tgattttaaa aatttcatca aaaaaataga agcaggtgct
     2461 gattcggcca tcactcaata tttctataat gcggatgctt attttcattt cattgatcaa
     2521 tgtgaagccg ctaatttaag gattccagtc gttccaggca tcatgccaat caataaattt
     2581 actcaattag taagattttc cgatacctgt ggcgcagaaa ttccccgctg gattagaaaa
     2641 aaactggaag gatatggcga tgactctgcg tcaatcacag cgtttggatt agatgtagtc
     2701 actgaattat gt
//
LOCUS       DAOQLJ010000244         2699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_22380_length_2699_cov_8.748109, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000244 DAOQLJ010000000
VERSION     DAOQLJ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22380_length_2699_cov_8.748109"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..1664)
                     /locus_tag="PK522_11555"
     CDS             complement(249..1664)
                     /locus_tag="PK522_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC42411.1"
                     /translation="MIQVSSFDQLGLTQELLKAIADQGYTEPTPIQAKVIPPIIEGRD
                     IMGSAETGTGKTASFTLPMLYRLQPHANSSTSPAKHPTRALILAPTRELATQIYGSVK
                     EYGKYLQLRCAVIYGGVNIDPQIKELQAGVEILVATPGRLLDHIEQRTANLSKIEIII
                     LDEADRMLDMGFLPDIKRILALLPAQRQSLIFSATFNDEIRKLADKLLKQPLLIEVAK
                     RNAINESIAHVVHMVSTRYKFDLLLQLLKQADSNPILIFVRTKQGVDYLVKQLLQSEI
                     LVAAIHGDKNQQQRTQALSDFKEGKIKALVATDIAARGLDIENLNCVINFELPTTPED
                     YIHRIGRTGRAGCKGNAISLISASEKELLINIEKLLGTKLEIKEVPGFGVEETGDNRS
                     SNRLSQSPTRNKERQPQSNSYPKKRKNTPPIDPIFTQPYVPPVLTDKTNVADGITPRT
                     FKSTKKVIAALFAPPINQKLK"
     gene            complement(1701..2474)
                     /locus_tag="PK522_11560"
     CDS             complement(1701..2474)
                     /locus_tag="PK522_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42412.1"
                     /translation="MKLRYFPSPNTAYTHRGRDYFIAGFTFFCVAVSLVIDAHANLLI
                     QNILGVIAWVFLVGLLVGENREIRVQVVIAVAFATAGEHFASIYMGGYTYRFENVPLY
                     VPPGHGMVYLTAVALARSGFFLKYARKIALFVVVTCGIWSLWGISGIPEHGDQVGAIL
                     YCVFLIYLFKGRSPMVYLAAFFITTWLEIIGTAAGTWKWATLEPIFQLSQGNPPSGVS
                     AWYCLVDAVAIGCAPIVLKGIRRMTDWAKSARSVLLNRR"
BASE COUNT          828 a          541 c          492 g          838 t
ORIGIN      
        1 aaaaatatcg cgtaaaactg ctctaattca tgacccatca actttagtcc catttttgaa
       61 tatgcagaga ctaacactcg attgactatt tttatctatg gaacaaatac caaaatgctg
      121 atttattctt actaaagtag tgcttaagaa aaaattgagg aaaaactaag tagaagaact
      181 tagaaattgg aggcgctaaa actacagaaa agccttatat cgataaatct caaatcaaat
      241 gagttgtttt actttaactt ctgattgata ggaggtgcaa ataaagcagc aataactttc
      301 ttggttgatt taaaagttct aggagtgatt ccatccgcta cattcgtttt atcagttaat
      361 acgggaggta cataaggctg ggtaaaaata gggtcaattg gtggggtatt ttttcgtttc
      421 ttaggataag aatttgattg gggttgcctt tctttatttc tagtaggcga ttgagatagc
      481 cgattagatg atcgattatc gccagtttct tctactccaa atccaggtac ctccttgatt
      541 tctaattttg ttccaagaag cttctctata ttgatgagta attctttctc agaagcgctt
      601 attaatgaga tggcattacc tttacaaccg gctcttccag tacgtccaat tcgatgaata
      661 taatcttcgg gtgtggtcgg taattcaaaa ttaatgacac aattcaaatt ttcaatatct
      721 aatccccgcg cagcaatatc ggttgcgaca agcgctttta ttttcccttc tttgaaatct
      781 gatagtgctt gtgtacgctg ttgctgattc ttatcaccat gaatcgcagc aaccaaaatt
      841 tccgattgaa gtaattgttt aacgagataa tctacacctt gctttgttct cacaaagatc
      901 aaaattggat ttgaatcagc ctgcttcagt aactgtagta gcaagtcgaa cttatatcga
      961 gttgatacca tgtgtaccac atgggcaatc gattcgttaa tcgcattacg ctttgctact
     1021 tcaatcagta acggttgttt taataattta tctgcaagct ttctaatttc atcattaaag
     1081 gttgccgaaa aaataaggct ttgccgctgt gcaggcaata aagccaaaat ccgtttgata
     1141 tcgggtaaga atcccatatc taacatacga tccgcttcat ctaagatgat tatttcaatt
     1201 tttgataaat tagcggttct ctgctcaata tgatccaata agcgacccgg cgttgcgact
     1261 agaatctcaa cgccagcctg taattccttg atttgaggat caatatttac ccctccatag
     1321 atgacagcgc accgaagctg aagatatttt ccgtattctt tcacgctacc ataaatctgg
     1381 gttgcgagtt cacgagtagg ggcaagaatt aatgcacgcg tcggatgttt agctggtgag
     1441 gtacttgaat ttgcatgagg ttgtaatcga tataacatcg gtaaggtaaa gctggctgtt
     1501 ttacctgtac cagtttcagc gctccccatg atatctctac cttcaataat cggtggaata
     1561 actttggctt gtatgggtgt cggttcagta tagccctgat cagcaatcgc ttttagtaac
     1621 tcttgtgtta atcctaattg atcaaatgat gatacttgaa tcatattttg ttaatcgtaa
     1681 aaaaaatgtt gatgaatgcg ctatctccta ttcagtaaca ctgatctcgc tgattttgcc
     1741 caatctgtca ttctacgaat gcctttaagc acaattggtg cacatccgat cgcaacagca
     1801 tcgaccagac aataccatgc tgatactcca ctgggtgggt taccttgtga taactgaaaa
     1861 atgggttcaa gcgttgccca tttccaagtt ccagctgcgg taccaattat ttctagccat
     1921 gttgtaataa aaaatgcggc tagataaacc atgggcgaac gccccttaaa gagatagatt
     1981 aaaaaaacgc agtataaaat tgctcccact tgatctccgt gctctggaat accactaatt
     2041 ccccagagag accatatgcc acaagtcacc acgacaaaca atgcgatttt ccgagcatat
     2101 ttaagaaaaa aacctgaacg tgctaatgcg actgcagtta gatataccat gccatgaccg
     2161 ggaggaacgt acaaagggac attctcaaaa cgataggtat aaccccccat ataaatcgat
     2221 gcaaaatgtt cacctgccgt agcaaatgca actgctatca caacttgaac tctaatttcc
     2281 cgattctcac cgactaatag acccactaaa aaaacccaag cgataacacc caaaatattc
     2341 tgtataagta ggtttgcgtg agcatcgatg actaggctta ctgcaacgca gaagaaagta
     2401 aatcctgcaa taaaataatc tctacctcta tgggtatagg ctgtattagg cgaagggaaa
     2461 taacgaagtt tcatctaatt ctcaaaataa aaacccgcta tgatagcggg tttgaaacca
     2521 gttatacgat taagagaaaa acgatcattt ctatctctaa taaaaagctt ttatttttta
     2581 taactgaaga agttagctta cttcttcagt catgttttat tactcttaat taaagctatg
     2641 attgttactc ttttcctttt ttatcgtctt ttttagctac cttagcgcta gcttggaat
//
LOCUS       DAOQLJ010000245         2662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_22986_length_2662_cov_6.117376, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000245 DAOQLJ010000000
VERSION     DAOQLJ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22986_length_2662_cov_6.117376"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PK522_11565"
     CDS             <1..807
                     /locus_tag="PK522_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNC42413.1"
                     /translation="YLIEECMLAANVCASDFLQRHQQPTLYRIHEGPVPEKLEALRNF
                     LKEFGLQLGGGDRPGAKHYAKTLLKIKTRPDVQLLQTVMLQSLQQALYQPENLGHFGL
                     AYESYTHFTSPIRRYPDLLVHRAIKAVLNHATYTPQTNWAELGKHCSMTERRADEATR
                     EVETWLKCFYMQDKVGKDFNGVISGVTAFGLFVSLDQIFVEGLVHISELPSDYFHFDN
                     IKHLLIGERTRKIYRLGDKIQVKLARVDLDERKIDFLLSVTKITKQVKKH"
     gene            859..1599
                     /gene="rlmB"
                     /locus_tag="PK522_11570"
     CDS             859..1599
                     /gene="rlmB"
                     /locus_tag="PK522_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNC42414.1"
                     /translation="MSTARTIFGFHAITSRIRQHPESIQEIFLDAHRNDQRAQNLIAL
                     AKNQGIRLLICDAEKLNKIVGTTHHQGVVASVLAIQNRATIETILDDISDSTLLLLLD
                     GVKDPYNLGACLRIADAFGAQAVVVPKDNAVGLTSTVYKVASGSAESIPLITVTNFAR
                     TIRLLKQQGIWVIGAAVEANDTLDHIKLSGPTAWILGSEGEGMRKLTQENCDQLVRIP
                     MIGHVNSLNVSVAAGICLFETLRQRNRS"
     gene            1753..>2662
                     /locus_tag="PK522_11575"
     CDS             1753..>2662
                     /locus_tag="PK522_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNC42415.1"
                     /translation="MRTALIFPFWLLLTSTVIFAQQKNSPVLMMDPVTVTATRSDRLM
                     VEIPQAINTIKRDQFTDHQPGVTLDEFGRNLPGVFFQNQFNFTQDLRIAIRGFGARSP
                     FGVRGIQMRVDDIPQTLPDGQTQLDAIDPAMIERMEVLRGPSASLYGNASGGMIHITT
                     RNPQHAFEVSPRLVFGQYGLIKSELYAGGRTENFNYGLFGSYLQQAGWRDHSRMENLF
                     SQVKINFKTGSDSDWMVLLHKFYSPDSKDPGALTLAESRANPKQASANNQLFNASESI
                     NQEQFAVRYRKNVAPGQLITITTHALH"
BASE COUNT          747 a          549 c          589 g          777 t
ORIGIN      
        1 tatttaatcg aagaatgtat gctggctgcc aatgtttgtg cctcagattt tttgcagcgt
       61 catcaacagc cgacgttgta tcgcattcat gaaggcccag ttcctgaaaa attagaagca
      121 ttacgcaatt ttctaaaaga atttggacta caactcggag gaggtgatcg acccggcgct
      181 aaacattacg ccaaaacgct gttgaaaatc aaaactagac cggatgttca gctattgcaa
      241 acagttatgc tgcaatccct ccaacaagcc ttatatcaac ctgagaattt agggcatttt
      301 ggcttagctt atgagtccta tacccatttc acttcgccta ttcgaagata tcctgatttg
      361 ctcgtacatc gtgcaattaa agcagtactc aatcacgcga cctatactcc ccaaacaaat
      421 tgggcggaac ttggaaagca ctgttcgatg actgagcgca gggcagatga agcaacacgg
      481 gaggttgaaa cttggctcaa atgtttttat atgcaagaca aagttggtaa agattttaat
      541 ggggtcatta gtggtgtaac ggcgttcggg ttgttcgtct cccttgatca aattttcgta
      601 gaagggcttg ttcatatttc agaacttccc agtgattact tccattttga taatatcaaa
      661 cacctgttga ttggcgaacg aacccggaaa atatatcgat tgggcgataa aatacaagtc
      721 aagctcgcac gggttgatct cgacgagcgc aaaatcgatt ttttattaag cgtgacaaaa
      781 ataacgaaac aggttaagaa acactagttt ttgtatcaat tcaactattt aacctcgatt
      841 taattctccg actaatccat gtctactgca agaactattt ttggcttcca tgcaattact
      901 agccgtattc gccagcaccc ggagagcatt caagaaatat ttttagatgc tcatcgtaac
      961 gatcagcgcg cacaaaattt aatcgcactc gcaaaaaatc aaggtattcg attattaatc
     1021 tgtgatgcag agaagttgaa taaaatagtg ggtacgaccc atcaccaggg agttgttgca
     1081 agcgttctcg ctatccagaa tcgggcgacc attgaaacta ttctggatga tatcagcgat
     1141 agcacattgt tattattgct cgatggtgtt aaagatccct acaatctcgg tgcttgctta
     1201 agaattgccg atgcatttgg tgctcaagcc gtagtcgtgc ccaaggataa tgcggtagga
     1261 ttaacgtcta ctgtttataa agtagcgagt gggagtgctg agagcattcc gctcatcacg
     1321 gtcaccaatt ttgcgcggac tatccgatta ttgaaacaac agggcatttg ggtcatcggt
     1381 gcagctgttg aagccaacga tactttggat catattaaat tgagtggtcc aactgcttgg
     1441 atattaggtt ctgaaggtga aggcatgaga aaattgacgc aagaaaactg cgatcagttg
     1501 gttcgcatcc cgatgattgg ccatgttaat agtttgaacg tctccgttgc agcgggtatt
     1561 tgtttgtttg aaacgttgcg tcaaaggaat cgaagctagc cgatcattgg gtattttaaa
     1621 taactgcttt tgtcagtgat ggttatcatg cagtaataaa ccgatcaaag tagatcaact
     1681 cggaactgcc tgagagtaaa acttttgatt ctagttaaaa ttagctgttg agaatcatta
     1741 cgaatttatg ttgtgcgaac tgcccttatt tttccatttt ggctgctttt gacatcaacg
     1801 gttatttttg ctcagcaaaa aaactcaccg gttctgatga tggacccggt tactgttaca
     1861 gcaactcgat ccgatcgatt aatggttgag ataccgcaag ccattaacac gatcaagcgg
     1921 gatcaattta ctgatcatca acccggcgtt acattagatg aattcggacg taacctaccg
     1981 ggagtatttt ttcaaaatca atttaatttt acgcaggatc tacggattgc gatccgcggt
     2041 tttggtgcac gttctccttt tggtgtgcgt ggtattcaaa tgcgtgtcga tgatattcct
     2101 caaaccttac cggacgggca aactcagctt gatgcgatcg atcctgctat gatcgagcgg
     2161 atggaagtat tgcgcggtcc ctcggcatct ttgtatggta atgcgtctgg tggcatgatt
     2221 cacatcacga cacgaaatcc gcagcatgcg tttgaagtat cgccgcggtt ggtttttggg
     2281 cagtatgggc ttatcaaatc agagctctat gcagggggtc gtaccgaaaa tttcaactat
     2341 ggtttgttcg gttcctatct gcaacaagcc ggttggagag accatagtcg tatggagaat
     2401 ctcttttctc aagtaaaaat caattttaaa acgggctccg attcggattg gatggtatta
     2461 cttcataaat tctattcacc agattccaaa gatccgggcg ctttaacctt ggctgaatca
     2521 cgcgccaatc ctaagcaagc atcggctaat aatcaattat tcaatgccag tgagtcgatt
     2581 aatcaagagc aatttgctgt tcgttatcgc aaaaatgttg cgccaggcca gctaattacg
     2641 attactacgc atgcacttca tc
//
LOCUS       DAOQLJ010000246         2648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_23211_length_2648_cov_5.600848, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000246 DAOQLJ010000000
VERSION     DAOQLJ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23211_length_2648_cov_5.600848"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..1593)
                     /locus_tag="PK522_11580"
     CDS             complement(208..1593)
                     /locus_tag="PK522_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNC42416.1"
                     /translation="MNIQKIIQERYKENFSLHDRYLNSQMVKVLKTIGFDRHYVKAEG
                     AYLYDEKGNRYLDLLSGFGVFALGRNHPVIVQALQDVLSIDLPNLVQLDVSILSGLLA
                     EKLVSLSPKGLDRVFFANSGTETIEAAIKFSRYATGKPKIIYCRGGYHGLSLGSLSAT
                     GDTHYKEGFGPLVPDFIEIPFGDLSALEEALSQRDVAAFITEPIQGHGVWIPADDYLP
                     KAAELVRHYGALFIADEVQTGLGRTGKWWAIDHWSITPDIICMAKALSGGFVPIGALV
                     CRNWIFDRVFNRMDRAVIHGSTFGKNNLAMAAGLATLDVLESENLIDKSAQSGKILLD
                     ALQPLAQQYECVKEIRGKGMMIALEFKEPQSLSLRMSWKILEAANKGLFSQLITVPLF
                     ARHRILSQVAGNGMNIVKFIPPFILNQSDLNWIINAVQDVVIAAHRGPKAVWDLGKVF
                     ATKALRMKAGS"
     gene            2022..>2648
                     /locus_tag="PK522_11585"
     CDS             2022..>2648
                     /locus_tag="PK522_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC42417.1"
                     /translation="MNLVLLSLKMLRRDWRAGELRILALALIIAVSSITTVGFFADRV
                     QLALAQESNRLLGADLVVTSDRPIPIEFATQAEHLQLKIVNLIRFPSMVSKGDHNLLA
                     EIRAVSPGYPLRGELKMMDRPNENATEENIYLAEGIPTQGTIWIDEKLLLGLETALDE
                     KLEVGIAEMTVTSIVAREPDHSVGFINMGPRLLMNIEDLAETQLIQPGS"
BASE COUNT          760 a          509 c          610 g          769 t
ORIGIN      
        1 gacaatcaat gtgagcaaca tcaccgaagc agtgataggt tttaatgttg ggaattgacg
       61 gtagacgggg cctattaatt tgaactcaat ttgttggtgt cttgggtgac taatcatgtg
      121 tttattttta ttactgtcat caatgctgaa attaaatcat agaaataggg ttttaaaaac
      181 aaggaaattt aagtcataac tgcaatatca agagcccgct ttcattctta atgcctttgt
      241 tgcaaaaact ttaccgagat cccaaactgc tttgggtcca cggtgtgccg cgatgaccac
      301 atcttgaacg gcgttaatga tccaattgag atccgattga ttgagaatga acggcgggat
      361 gaatttgacg atattcatgc cattgcctgc tacttgagac agaatgcgat gccgtgcaaa
      421 tagaggtacc gtaatgagtt gagaaaataa tcctttattg gcggcttcta aaattttcca
      481 ggacattctg agcgataagc tttggggttc tttaaattca agcgcgatca tcatgccctt
      541 acctcgaatt tctttgacgc attcgtattg ctgagcaagg ggttgcagcg catcgagtag
      601 gatttttccc gattgtgcgg atttatcgat taggttttcc gattctaaaa catctaaggt
      661 agctaacccc gctgccatcg cgaggttatt ctttccaaac gtactaccat gtatcacggc
      721 gcgatccata cgattgaata cgcgatcaaa gatccaattt ctgcaaacga gtgcgccaat
      781 ggggacaaaa ccacccgaaa gggcttttgc catacaaatg atatcggggg tgatagacca
      841 atggtcgatc gcccaccatt ttccagttct gcctaatccg gtttgaactt catcggcaat
      901 gaataatgcg ccatagtgac gaacgagttc tgcagctttg ggtagataat catcggcggg
      961 gatccataca ccgtgtcctt ggatcggttc tgtaatgaaa gcagcgacat ctctttggga
     1021 aagcgcttct tcgagtgcgg ataaatcgcc gaaaggtatc tcaatgaaat caggaactaa
     1081 gggaccgaat ccctctttgt aatgggtatc acccgttgct gagagtgaac caagacttag
     1141 tccatgatac ccgccgcgac agtagatgat cttcggtttt ccagtcgcgt aacggctaaa
     1201 tttaattgca gcctcaatgg tttcagtgcc tgagttagca aagaaaacac gatctagccc
     1261 tttcggactc agtgacacca atttttcagc cagcaatcct gataggatag agacatcgag
     1321 ttgcacgaga ttgggtaaat caatacttaa tacatcttgt aacgcttgta caataaccgg
     1381 gtgattacga cctaacgcaa aaacaccgaa accactgagc aaatcgaggt agcgattgcc
     1441 tttttcatca tataaatagg caccttcggc tttaacataa tgtcgatcaa acccgattgt
     1501 tttgagcact ttcaccattt ggctattgag atagcggtcg tggagtgaaa aattctcttt
     1561 gtaacgttct tggatgatct tctggatgtt catagactaa atggccaaat atgtggtttc
     1621 aggttataag tgctgatgat tgtgagcgat tgagcaacgg tgaaatagga atttgtaatt
     1681 aaacttggta cacatcagct aatagggtat ttaaagcaaa gcgatagggt aacagcaatt
     1741 gggagatata acctagatta gtctatgcag atattgatta caaagtgtca caattgttgt
     1801 ggaaattttc aaagcattat tatggtgtct ccctaagact gtcaatagtt aagaaaatgt
     1861 tttacgcact gcgcttttct cgaattcacc gatcatttga ttgctcaggg caggcagttt
     1921 taatgctgtg attaaaatcg agatcgatct ggggttaaat tttttcaatt tcgagtgatt
     1981 aaagcactgt ttgtatgacc aaaattaatg gatgttaaag aatgaatctc gttttacttt
     2041 cgttaaaaat gttgcgtcgt gattggcgcg caggtgaatt aaggattctg gcattagcgc
     2101 tgattatcgc agtcagtagc attactacgg taggtttttt tgctgatcgt gtgcagcttg
     2161 cgctagctca agaaagcaat cgtcttttag gcgctgattt agttgtaacc agtgatcgac
     2221 ccattccaat cgagtttgcg actcaagccg agcatcttca actaaaaatt gtgaatttga
     2281 ttcgttttcc gagcatggta agcaaaggag atcataacct gctagccgaa atcagagcgg
     2341 tttcgccagg atatccattg cgtggggaat tgaaaatgat ggatcgtcct aatgagaatg
     2401 caacggagga gaacatctat ctggcagaag ggattccaac tcagggtacg atttggatcg
     2461 atgagaaatt acttttaggg ttagaaactg cgctggacga gaaattggaa gtaggtattg
     2521 ctgaaatgac cgtcacatcg atcgttgcac gcgaacccga tcattcagtc ggatttatca
     2581 atatggggcc tagattattg atgaatattg aagatttagc tgaaacccag ttaattcagc
     2641 ctggaagt
//
LOCUS       DAOQLJ010000247         2628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_23526_length_2628_cov_6.112709, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000247 DAOQLJ010000000
VERSION     DAOQLJ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23526_length_2628_cov_6.112709"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..545
                     /locus_tag="PK522_11590"
     CDS             126..545
                     /locus_tag="PK522_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42418.1"
                     /translation="MKAILSISISLLILTGCHTFGIHKLGGESGGKPNCFNRSTDNEC
                     SAPNLENVVLTSEVETALKKLGIPDVSFIAVVSKDGKITFAKSAATEVKRFQLPSTFT
                     IESIQSYSLIKFKGSTCKSWIPGHVEGGAWISGYCDD"
     gene            611..772
                     /locus_tag="PK522_11595"
     CDS             611..772
                     /locus_tag="PK522_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42419.1"
                     /translation="MNNDDLEVNSQNFEVMNADINEERTPMPRSGSWHWVPGHVEGGA
                     WIAGYWEQD"
     gene            886..1635
                     /locus_tag="PK522_11600"
     CDS             886..1635
                     /locus_tag="PK522_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNC42420.1"
                     /translation="MNTGREEFIDLSFNTSLREFLLIKEFQGILLSDLEKIIHTCHQQ
                     CYKAGEEIVRYQDHTNSVFFILKGIIRIHYYSLSGDEVILCDLPEGEMFGELTAIDGL
                     ARSATAVALKDSLLASLPSSAFLELVHTYPEFCTAILKRLVGQVRRLTERVFDFSTLA
                     VRNRIHAELLRLTHKNKVSENLAIISPAPTHEELANYVSTRREEVSREISVLARQGII
                     ECRGRAWHILDVAKLAHMVKDVRGSFDTEKV"
     gene            complement(1671..>2628)
                     /locus_tag="PK522_11605"
     CDS             complement(1671..>2628)
                     /locus_tag="PK522_11605"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNC42421.1"
                     /translation="IAERAGRLRIVMADGSPSEPTISGLPNLLARDQGGLLGIALDPD
                     FARNQQLYFSYAEQQGNVAGTAVARAKLVGNQLKNVQVIFRQLPKTEGTKHFGSRLVV
                     APNGNLFVTLGDRFTYMDQAQHLDNHIGKLIRIRKDGSVPTDNPFAKHSVAKPEIWSY
                     GHRHIQGAAIHPGTGVLWIHEHGPRGGDEINIPLPGKNYGWPLASYGMHYSMEPIKDE
                     HAEQGFEEPIYYWNPSIAPSGMMFYRGNLFPEWKGNLFIGSLVGRHLVRLVVDSNQVI
                     SEEKLLMNQARIRDVAEGPDGAIYLLTDDTNGLLLKLSRPTP"
BASE COUNT          767 a          483 c          608 g          770 t
ORIGIN      
        1 atttattaga tcaagatttt ttatatagaa gtataaattt tcagcagtct gaagcaattg
       61 ctagccgctt tatccctagc agaacgattt ttttacgact aacacttaac ttttaaggag
      121 gaaaaatgaa agcaatattg agtatatcca ttagtttatt aatcttaaca gggtgtcata
      181 catttggtat tcataaatta gggggggaga gtgggggaaa accaaactgc tttaatagat
      241 ctacagataa cgaatgtagt gcgccaaatt tagaaaatgt agtattaaca agtgaagttg
      301 aaacagcttt aaaaaaatta ggtatccctg atgtatcatt tatagcagtt gttagtaaag
      361 atggaaaaat aacatttgct aaatctgctg caactgaagt taaaagattc caacttccaa
      421 gtacatttac tatagaaagt attcaatctt attctttaat taaatttaaa ggatcaactt
      481 gtaaatcttg gatacctggg catgttgaag gcggtgcatg gataagtggt tattgtgatg
      541 attaatctaa tttaaattat ttttttatgt aatctgctat ttcttttttt taacaaatat
      601 gagggaaaaa atgaataatg atgatttaga agtaaactca caaaacttcg aagttatgaa
      661 tgcagatata aatgaagaac gaacacccat gccaagaagt gggagttggc attgggtgcc
      721 cggacatgtt gaaggggggg cgtggattgc tggttattgg gagcaagatt aatttttaaa
      781 taaaattaga acattaataa gcttatgatc tgtatagata gttatacgga ccataagctt
      841 atccataaaa gctatgagcc aaatgttttt gcttaaaaaa gaaaaatgaa tacaggaagg
      901 gaagagttta tagacctcag ttttaacacg agcttgcgtg aatttctcct aataaaggag
      961 ttccagggta ttttactcag tgatttagaa aaaataattc atacttgcca tcaacaatgt
     1021 tacaaagccg gagaggaaat tgttagatac caagatcaca ctaacagtgt tttctttatc
     1081 ttaaagggaa taattcgaat ccattattat tctctgtccg gggatgaggt tattttatgt
     1141 gatctccccg agggagagat gtttggtgaa ttaaccgcta tcgatggatt agctcgatct
     1201 gcgacggccg ttgcattaaa agattcgctg ctggcttctt tgcccagcag cgcttttctc
     1261 gagctagtcc atacttatcc tgagttttgt acggctatct taaagcgatt ggttggtcag
     1321 gtgaggcgct tgactgagcg ggtgtttgat tttagtacct tggccgttcg gaaccgcata
     1381 catgcagaat tactgcgctt aacccataaa aacaaggtgt cggaaaactt ggcgatcatt
     1441 tcgccggcac caacccatga ggaattagct aattatgtaa gtactcggcg tgaggaagtt
     1501 tcacgcgaga tatctgtcct tgcacgtcaa ggtatcatcg agtgcagagg acgggcatgg
     1561 catatcttgg atgtagctaa gttagcacat atggtcaaag atgttcgtgg ttcattcgat
     1621 actgagaaag tttgactcac tatgagagaa ctgttccacg atttgtgttt ttagggtgtg
     1681 gggcgcgata gtttaagcaa taagccattt gtatcatcag tgagtagata aatagcacca
     1741 tcaggtcctt ctgcgacatc gcgaatacga gcttggttca tgagcaattt ttcttcactg
     1801 atcacctggt tgctgtcaac cactaaacgg accagatgcc gtcctaccag agagccaatg
     1861 aataaattac ctttccactc tgggaacaaa ttaccacggt aaaacatcat cccagaaggg
     1921 gcaatggacg ggttccaata gtaaatcggc tcttcgaagc cctgttcagc atgttcatct
     1981 ttgatcggct ccatagaata atgcataccg tagcttgcta aaggccagcc gtaattttta
     2041 cctgggagtg gaatattgat ttcgtcaccg cctcgtggac catgttcgtg aatccataat
     2101 actcccgttc cggggtggat ggctgcgcct tgaatatggc ggtgaccata ggaccagatt
     2161 tcgggtttgg ccactgagtg tttagcaaag gggttatcag taggcacgct gccatctttg
     2221 cggatacgga tgagtttgcc aatatggttg tcgagatgct gcgcttgatc catatacgta
     2281 aaacggtctc ctagagtgac aaataggttg ccattgggtg caaccaccag tcgtgaccca
     2341 aagtgtttgg taccctcggt tttcggcaat tgcctgaaga tgacctgcac attcttcagt
     2401 tgattgccga ccaatttggc acgagcaacg gcggtgccag ccacattgcc ttgttgttcc
     2461 gcataggaga aatagagttg ctggttgcgc gcaaaatcgg ggtcaagcgc gatgcctagt
     2521 agccctcctt gatcacgggc aagtaaattg ggtaatcctg aaatggttgg ttctgaggga
     2581 ctgccatctg ccatgacgat acgcaatcga ccggcgcgtt cagcgatc
//
LOCUS       DAOQLJ010000248         2562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_24599_length_2562_cov_7.652573, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000248 DAOQLJ010000000
VERSION     DAOQLJ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24599_length_2562_cov_7.652573"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            409..1032
                     /locus_tag="PK522_11610"
     CDS             409..1032
                     /locus_tag="PK522_11610"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HNC42422.1"
                     /translation="MAAKSVQSIASKLLSRIHGHGRGWAFSANDFVVEFNSKNLDQAL
                     SQLVKDSKIRRVFRGLYDYPRYSELLQQELSPDCDQVAHALARKFNWRIQPSGEAALN
                     LLGLTTQVPAKYIYHSDGPNRQYKIGKYTLAFKNTSLKNIGFKYRESGLIVQALKALG
                     KDHIDEDIIASIRQQLTPAIQSRLLKDTTTVTDWVYNHIKSICRGED"
     gene            1032..2024
                     /locus_tag="PK522_11615"
     CDS             1032..2024
                     /locus_tag="PK522_11615"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNC42423.1"
                     /translation="MDKVARLSSTERQELFSEAAARKGVTPAIIEKDFWVTWVLSKLF
                     ANDELARLLMFKGGTSLSKVFRLIERFSEDIDLILDWNVLTSEEPLEDRSRRKQETLN
                     NTINEQAKTYIAQILHPLIASQIEPVCRCVLDVDDGFVLNIHYPAAFPDLYLRPEVRL
                     EIGPLAAWFPSGTFEISSYAAAEFPQVFAQSSCRVKAILAERTFWEKATILHHEANRP
                     TDSLQPSRYSRHYFDLARMAMSWVRGSALSRLDLLADVIAFKQRFYPRGWAQYDHAVA
                     GTLKLVPPPHVLSVVRQDYQNMQQMIFGHSLTFEEMMATLNDLEKEINALSNQS"
     gene            complement(2236..>2562)
                     /locus_tag="PK522_11620"
     CDS             complement(2236..>2562)
                     /locus_tag="PK522_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNC42424.1"
                     /translation="VEIGAYQKAVTTTRENPSAEQVADITLRLNIENNSQFVRGKKRA
                     RENIERYCLGKYHATRLSSGDYSLRINYKNREDLDELMADLLRDMSHEAEMRHCYIET
                     EVWEDG"
BASE COUNT          738 a          570 c          516 g          738 t
ORIGIN      
        1 cgcctatttg atagcacaga tggcactttt tacacgaacg tggtaactac ccctatttgg
       61 gtatggagac tgggtgagtc caggttcagc gacgttgatt ggcgcgggcc ggagtgccag
      121 agataacatt ccaaagctgg tggactatat caggcaatct catcagtgag ctgaaagacg
      181 ccagagtagc caaaacacct caaaatcaca cgtttacatt gaaatttagt ctatttataa
      241 caatagctta aatcactttt tgtgaaaaaa attcagaatc ccgttggacc ctctatatcg
      301 cataatagcg aaaaattacc cctgattgcc ttcttccttg aacatcaaaa tgcatacaaa
      361 gcaggatgaa ttattttaga aaaagtgata tatttttcta aaatttttat ggcggctaaa
      421 tcggtgcaat ccattgctag taaattgtta tcacgcattc acggtcatgg cagggggtgg
      481 gcattctctg caaacgattt tgttgtagag ttcaatagca aaaatcttga tcaggcactt
      541 tcacaattgg tcaaggattc caaaattcgc cgtgtttttc gagggctata tgactatcca
      601 cgctacagtg agcttcttca acaggagctt agcccagact gcgaccaagt tgcacatgca
      661 ttagctcgca aattcaactg gcgcattcag ccttctggtg aagcagctct gaatttgtta
      721 gggctgacaa cacaggtgcc agccaaatat atttatcact ctgacggccc aaaccggcaa
      781 tacaagattg gaaagtacac cttagctttc aaaaacacct cgctaaaaaa tattggcttc
      841 aaatatcgcg aaagcggcct tatcgtccaa gcactcaaag ctctaggcaa agaccatatt
      901 gatgaagata ttatagcttc catcaggcaa cagttgacgc ctgcaataca gtcgcgcttg
      961 ctgaaagata caaccacagt cactgattgg gtttataacc atatcaaatc aatttgtcga
     1021 ggagaagatt gatggataaa gttgccagat taagttccac tgagcggcaa gaattattca
     1081 gcgaagctgc tgcccgtaaa ggcgtaactc cagcgattat tgaaaaagac ttctgggtta
     1141 cttgggtgct gagtaaatta tttgctaacg atgagcttgc acgtttgttg atgttcaaag
     1201 gtggcaccag tctatcgaag gtctttagac tgatagagcg attttctgaa gacattgact
     1261 tgattctgga ctggaacgta ctgactagtg aagaacctct tgaagacaga tcaaggcgca
     1321 agcaagaaac tttgaataac acaattaacg aacaggccaa gacttatatt gctcagattc
     1381 ttcatccact gattgcatct cagattgaac cagtgtgccg ttgcgttctg gatgtagacg
     1441 atggctttgt cctaaacatc cattatcccg ctgctttccc cgacttatat ttgcggccgg
     1501 aagtccgcct agagatagga ccgctggctg catggtttcc atcaggtact tttgaaatat
     1561 cttcctatgc ggcagcagaa tttcctcagg tttttgctca gtcctcttgc agagttaagg
     1621 cgatccttgc cgagcggaca ttctgggaaa aagccactat tttgcaccat gaggccaatc
     1681 gaccaacaga ctcccttcaa ccatcccgct attctcgcca ttatttcgat ctggctcgga
     1741 tggcgatgtc ttgggtaaga ggatcggctc tttctcgtct tgatctgctt gcagatgtta
     1801 tcgcttttaa acagcgattt tacccacgag gttgggctca atatgatcat gctgtagcag
     1861 gcactctcaa gttagttcca ccacctcatg tgctgagtgt ggtccgccag gattatcaga
     1921 atatgcagca aatgattttt ggccattccc taacatttga ggaaatgatg gcaacgctta
     1981 atgatttgga aaaagaaata aacgctcttt ctaatcagtc atagcagagt aactcttccc
     2041 tcattttcta taggttgtaa tgttctaata aagtaggact ctttgaaagg tagcatttat
     2101 actcctagta gatatcttga ggagagcaac aaattcaacg aataagctga gctacatttt
     2161 atcatttaaa caaggtgccg tgaaattact tacactatgg ctataataca ctccctaaaa
     2221 actaacaaaa atattctaac catcttccca tacctcagtc tcaatataac aatgacgcat
     2281 ttctgcctcg tgactcatat cacgcaataa atcagccatt aactcatcta aatcctcacg
     2341 gtttttataa ttgatgcgta agctataatc gcctgacgat agccgtgttg catgatattt
     2401 acctaaacaa taacgctcta tattttccct tgctcgtttt ttaccacgta caaactgact
     2461 gttattttca atattcaagc gcagggtaat gtcagctact tgctcggctg atggattctc
     2521 cctcgtagtt gtcaccgctt tttgataagc ccctatttcg ac
//
LOCUS       DAOQLJ010000249         2540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_24971_length_2540_cov_4.551308, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000249 DAOQLJ010000000
VERSION     DAOQLJ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24971_length_2540_cov_4.551308"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /gene="trpC"
                     /locus_tag="PK522_11625"
     CDS             complement(<1..309)
                     /gene="trpC"
                     /locus_tag="PK522_11625"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646582.1"
                     /note="involved in tryptophan biosynthesis; amino acid
                     biosynthesis; converts
                     1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate to
                     C(1)-(3-indolyl)-glycerol 3-phosphate and carbon dioxide
                     and water; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HNC42425.1"
                     /translation="MSDILNKILTVKVQEVAAAKALKSLSNIQIVAESSGQARDFVGA
                     IRTKLNQGQPAVIAEIKQASPSKGLLRGRIVSHQAATDLTLGRTQDVVIEGFEPAAIA
                     A"
     gene            complement(324..1349)
                     /gene="trpD"
                     /locus_tag="PK522_11630"
     CDS             complement(324..1349)
                     /gene="trpD"
                     /locus_tag="PK522_11630"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNC42426.1"
                     /translation="MSPQEILTRIIQHQDIPHDDMLVLMRQIMQGELSPVLIAAIITG
                     LRVKRESIDEIAAAAQVMREFATSVEVLDTDHLIDTCGTGGDTSHTFNVSTASVFVAA
                     AAGANVAKHGGRSVSSKSGSADVLEALGVGLNQTPQQIAQSIADIGVGFMFAPNFHSA
                     MKHAAPVRRELGVRTLFNILGPLTNPAGAKNQLLGVFDAKLVNLLVSVLHRLGSRHVM
                     VVHGFDGLDEITITGKTLIGELKQGERLEYTIQPEDFGMRSAPIEAIRVSDLNQAKDM
                     LLAVLENEPGPARDIVSLNAGAAIYISGIAPSLSQGIAVAQETLASGAAKAKLQALVN
                     YSRREAH"
     gene            complement(1380..1946)
                     /locus_tag="PK522_11635"
     CDS             complement(1380..1946)
                     /locus_tag="PK522_11635"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNC42427.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGETVEVFRNDEITLERIKALNA
                     QRIVISPGPCTPNEAGVSLSLLKEYGGQVPTLGVCLGHQSIGQAFGGKIVHAKQIMHG
                     KTSPIYHHNIGVFHGLPNPFTATRYHSLVIERESLPDCLEITAWTEDGEIMGVRHKSL
                     PIEGVQFHPESILSEQGHQLLKNFLTSR"
     gene            complement(2060..>2540)
                     /locus_tag="PK522_11640"
     CDS             complement(2060..>2540)
                     /locus_tag="PK522_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42428.1"
                     /translation="IQMKKALTQADLVIPACQYACEYLARNHRKQESHWWQQQAERQL
                     AIDHQADVERTELADSDQIIAYESDDATSSYLAEKLRETGKITKAWIAQKIVQYYPEY
                     PVLVIVIECNGLFVNEMTLIEQLNKQLHLGCAFFVISKKGPHPSLAKQICALNDRII"
BASE COUNT          681 a          613 c          514 g          732 t
ORIGIN      
        1 cgctgcaatg gctgcgggtt caaatccttc gatgaccaca tcctgagtgc gacccagcgt
       61 taaatcagta gcggcttgat gagaaacgat ccgtcctcgc aatagccctt tactcggact
      121 tgcttgtttg atttctgcaa tgacagccgg ttgaccctgg tttagtttgg ttcgtatcgc
      181 gccgacaaaa tcccgagcct gtccagaaga ttctgctact atttgaatgt tggataacga
      241 ttttagcgct tttgcagccg caacttcttg cactttaaca gtaagaattt tattaagaat
      301 atcagacata gataacgggg tatttagtgt gcttcccggc gggaatagtt aaccaatgct
      361 tgtaattttg ctttggccgc accgcttgcc aatgtttctt gagcaaccgc aatgccttgc
      421 gatagtgaag gagcgattcc agaaatataa atcgctgcac ccgcattcag tgaaacaata
      481 tctcttgctg ggccaggctc attttcgaga acagcaagca acatatcctt ggcttggttt
      541 aaatcagaaa ctcggatagc ctcaatcggc gcactccgca ttccaaaatc ttccggttga
      601 atcgtgtact ctaggcgttc accttgtttt agttcaccta tcaaggtttt accggtaatc
      661 gtgatctcat ctaatccgtc aaacccatgc acgaccatca catgtcgact tcccagtcga
      721 tggagcacgc tgactaatag attcactaat tttgcatcaa aaacacctaa taactggttt
      781 ttagcaccgg caggattggt cagtgggccc aatatattga acaaagttct cacccctaat
      841 tcgcgccgta ccggtgcagc atgtttcatc gcactatgaa aattaggcgc aaacataaac
      901 ccaacgccaa tatccgcaat cgattgcgcg atttgttggg gtgtttgatt caaacctacg
      961 ccaagtgctt cgagcacatc cgcactcccc gatttacttg atactgaacg cccgccatgc
     1021 ttcgcgacat ttgcacccgc cgctgctgcg acgaacaccg atgcagtcga tacattgaaa
     1081 gtatgtgacg tatcaccgcc agtaccacag gtatcgatca agtgatcagt atccaacacc
     1141 tccacactcg tcgcaaattc acgcattact tgcgccgctg ccgcaatttc atcgatcgat
     1201 tctcgtttga cccgtaatcc agtaatgatc gcagcgatta gaacgggtga tagttcccct
     1261 tgcataattt gacgcatcaa gaccagcata tcatcatgcg gaatatcctg atgttggata
     1321 attcgagtta atatttcctg gggactcata tcaaaaaaat ttggaaaatt tagctaagct
     1381 tagcgactgg tcagaaagtt ttttaggagt tgatgccctt gttcactgag tatcgattcg
     1441 ggatgaaatt gtaccccttc aatgggcagt gatttatggc gaacccccat tatttcccca
     1501 tcttcggtcc aggccgttat ttccaggcaa tctgggagac tctctcgctc aatcaccaga
     1561 gaatgataac gggtcgcagt aaatggattg gggaggccgt gaaatacacc tatattgtga
     1621 tggtatatcg gtgacgtttt tccatgcatg atctgcttag cgtgcacaat ttttccacca
     1681 aatgcttgcc caatgctctg gtgtcctaga cataccccta acgtaggaac ctgaccaccg
     1741 tattctttca gcagcgataa ggacacgccc gcttcattag gcgtacaagg cccaggtgaa
     1801 ataacgattc gttgtgcgtt aagcgcttta attcgttcta gcgtaatttc atcatttcta
     1861 aatacctcga cggtttcacc gagctcacca aaatattgca cgaggttata agtaaatgaa
     1921 tcataattat caatcatcaa cagcatgcta cagctatttc ctttcaagat ctcagataga
     1981 aagactagtt tatcacagcc ttctatcaac ttgagttgaa tgcagtaatc aactcagtca
     2041 cattggtctt tcttgaatct cagatgattc gatcatttag cgcacaaatt tgcttagcca
     2101 aggatgggtg cggtcccttt tttgaaatga caaaaaatgc acaacccaaa tgaagctgtt
     2161 tattcaattg ttcaatcaat gtcatctcat tcacaaataa gccattacac tcaatgacaa
     2221 tgaccaacac aggatattca ggataatact gaactatttt ttgagcgatc cacgctttgg
     2281 ttatttttcc ggtttcgcgt agcttttcag caagataaga tgaggtagca tcatcacttt
     2341 cgtaagcaat gatttgatct gaatcggcta attctgttct ctcaacatcc gcctgatgat
     2401 cgatagctaa ttgacgttca gcctgttgtt gccaccaatg tgactcctgc ttgcggtgat
     2461 tgcgagctaa atactcacag gcatattgac aagctggaat aactaaatct gcctgtgtca
     2521 gcgctttttt catttgaatt
//
LOCUS       DAOQLJ010000250         2532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_25136_length_2532_cov_3.645943, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000250 DAOQLJ010000000
VERSION     DAOQLJ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25136_length_2532_cov_3.645943"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..1227
                     /locus_tag="PK522_11645"
     CDS             325..1227
                     /locus_tag="PK522_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42429.1"
                     /translation="MAYRIVKGTVSVDGKSPDGDTIAFTIDTRNEWMWPETEDGRFPQ
                     FNSKYQANIRFEAIDALELHYSIQSVYPGKTLRQPLDLAKQARNRLLDLCGFDRTALI
                     ESDDFKITDSKQQKIPVTLAYNGIDSYGRIIGFVFTQDMGFEVSDRNPTVQLTPEHVS
                     QSLNAQLLREGRVYPTFYNALYPQLRAVFSDIVVKARSQSLGIWERHQDEFVFDRKPS
                     LEEIEALVMMPKLFRRLSTHIAQNGAVSGFRDYLRDINDMTVDTREVRLSDFSSFVRT
                     QKLADGKYKLWMSHRPEELVFVKG"
     gene            complement(1409..>2532)
                     /locus_tag="PK522_11650"
     CDS             complement(1409..>2532)
                     /locus_tag="PK522_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC42430.1"
                     /translation="FYYLLQEYDPEFKELFKVAADFEETIERTPENHFLYAQLIATLT
                     RKEGLLPYDRHAVARVIDYSARLVEDSEKLSMHMRSIADLLHEADYWARANNSDAVRA
                     SDVQQAIDAQIRRQDRIRDHLYEAIQRDILMIGTQGAVTGQVNGLSVIELGGFAFAQP
                     TRITATTRFGKGDVINVEREAKLSGAIHSKGVFILSSFLAARYAKDQPLALSASLAFE
                     QSYGMIDGDSASLAELCALLSNLADAPVNQSLAVTGSINQLGQVQAIGAVNEKIEGFF
                     DICNARGLTGNQGVLIPATNVQHLMLRQDVVEAAAAGKFHIYAVADIDQAIALLTGLA
                     AGEIGANGEYPHGSINGLVAARVAELGKISQSYLGNKSH"
BASE COUNT          668 a          549 c          616 g          699 t
ORIGIN      
        1 tgcgcatcaa ggatttccgc ttgttcggtt tcaccgatgt gctgtttccg gggcaaggac
       61 tagtggatga ttgaagtaag gttcgttaga attttcataa caatttgagg aattgtattg
      121 aaacttatat ttaaacaatc gcatctttca atcgatcaat ttgatccaaa tgattttagt
      181 catttcaatt cgctgattga cgagatcagg cggatacaaa gtttataaca aacatttttt
      241 accttgaagg aggttgctga gattctctct gcaaaataga agtttcattt atgctgagta
      301 attaactgaa aaaggagtat cgatatggct tatagaattg ttaaaggtac agtttcagtc
      361 gatggaaaat ccccagatgg agacaccatt gcttttacga tagatacgcg taatgagtgg
      421 atgtggccag aaacagaaga cggacggttt cctcaattca attcaaagta tcaagcaaat
      481 atccgatttg aagctattga tgcgcttgaa ctccattact cgattcagag cgtttatcct
      541 ggaaaaacgc taagacaacc ccttgattta gcaaaacagg cacgtaaccg gcttcttgat
      601 ctttgcggtt ttgatcgcac ggcgcttatt gaaagtgatg attttaaaat taccgattca
      661 aagcaacaaa agattcctgt tacgcttgca tataatggta tcgattcata tggacgaatc
      721 attggctttg tatttacgca agatatggga tttgaagttt cagatcgtaa cccaacggtt
      781 caacttactc ccgaacatgt atcgcaatca ttgaatgcac aacttcttag agaaggtcgt
      841 gtttatccca cattttataa cgcactctat ccgcagttac gagcagtgtt tagtgatatt
      901 gttgttaaag cgaggtccca gtctttggga atttgggagc ggcatcaaga tgaatttgta
      961 tttgatagaa aacctagttt ggaggaaata gaagctttgg taatgatgcc taagctgttt
     1021 cgccgacttt caacccatat agcacagaac ggtgcagttt ctggatttag ggattatttg
     1081 cgtgatataa atgacatgac agttgatacc agagaagtca ggttgagtga tttctcatcc
     1141 tttgttagaa ctcagaagct ggcagatggt aaatataaac tttggatgag ccatcgacca
     1201 gaagagttag tatttgttaa aggctaatag gcaagaaatc tcagggttga ttatacagag
     1261 aggttgatag aaatacgtgg aaagtatttt atagggggta attttattca aactcttata
     1321 agataattgg cactgtcttt aaaacaaatc tgccactgtc catagccata gcgtatggat
     1381 gatctgctag ccggttcgga tttccgtttt agtgcgattt atttcccagg tacgattgac
     1441 tgattttccc aagttccgcc acgcgcgccg ctaccaatcc attgatactg ccgtgcggat
     1501 attcaccgtt tgcgccaatt tctcccgccg ctaagccggt caacagcgcg atggcttgat
     1561 cgatatccgc tactgcataa atatgaaatt tacccgccgc tgccgcctcg acgacatcct
     1621 ggcgtaacat caggtgctga acattagtgg cgggaatcaa tacgccttga ttaccggtca
     1681 gtccgcgtgc gttgcaaatg tcaaagaagc cttcgatttt ctcgttcacg gcaccgatgg
     1741 cttgcacttg gccaagctgg ttgatcgagc cggtcacagc cagcgactgg ttgaccggcg
     1801 catccgccag attggaaagc agggcgcaca attccgccag cgatgcgcta tcgccatcga
     1861 tcatgccgta cgactgctcg aacgccagac tggcggacag cgcgagcggc tgatccttgg
     1921 cgtaacgggc ggcgaggaag gaggagagaa tgaacacacc tttggaatgg atagcgccgg
     1981 agagtttggc ctcacgctcg acgttgatca catcaccttt accaaaacgc gtggtggcgg
     2041 tgatacgggt tggttgcgcg aaagcgaagc cgcccagttc aatgacggat aagccattga
     2101 cttggccggt taccgcgcct tgcgtgccga tcatcagaat atcgcgttga atcgcttcgt
     2161 atagatgatc tctgatgcga tcctgacggc ggatctgcgc atcgatcgct tgctggacat
     2221 cgcttgcccg cacggcgtcg gaattgttgg cacgcgccca gtaatcggct tcgtgcagca
     2281 gatcggcgat actgcgcata tgcatggaaa gtttttccga gtcttccacc agacgggcgc
     2341 tataatcgat tacccgggcg actgcatgcc ggtcgtaagg tagcaatcct tccttgcgcg
     2401 tcagcgtagc gatcaactgc gcgtacagaa aatggttttc tggcgtgcgt tcgatggttt
     2461 cctcgaaatc tgcggctact ttgaatagct ctttgaactc cggatcgtat tcctgcaata
     2521 ggtaataaaa ca
//
LOCUS       DAOQLJ010000251         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_27265_length_2421_cov_5.255706, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000251 DAOQLJ010000000
VERSION     DAOQLJ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27265_length_2421_cov_5.255706"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="PK522_11655"
     CDS             <1..1016
                     /locus_tag="PK522_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNC42431.1"
                     /translation="KGEIRTVTARGDLALDERTTIELFEQSRDSVVFISTTKLVRDIW
                     TRNVFAVPRGNGSGFFWDDAGHIVTNFHVIQGASEATVKLADGRDYKAALIGVSPSHD
                     IAVLKIGVGFKRPNPIPIGTSHNLKVGQKVFAIGNPFGLDWTLTTGIISALDRSLASD
                     NGTAIEHLIQTDAAINPGNSGGPLLDSAGRLIGINTAIYSPSGASAGIGFAVPVDTIM
                     RVVPQIIDKGKYIRPSLGISVDERFNDRIVQLLNIRGVVVLNVAPGSAAEKAGLKGAA
                     ISIEGNITPQDIIIAINGKKVESVSKLLARIDDFNVGDVVTLTVFRNEQTREVHITLQ
                     PGV"
     gene            1049..1570
                     /locus_tag="PK522_11660"
     CDS             1049..1570
                     /locus_tag="PK522_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC42432.1"
                     /translation="MDFSDFKLDSASVDQSQAICELLNSAYRGTDGWTTEVDIISGKR
                     ADRNEIEAAIKLPNSYFFVANQSNQLASCIHITKKDDHAQFGFFAVQPSLQGNGLGTY
                     VLKQAEIFAKTRLYSKKLLMIVVSQRTELIAFYERRGYRRTGQVENLPANIGIPKRIN
                     LTIDYLEKSIATT"
     gene            1710..1946
                     /gene="rpmB"
                     /locus_tag="PK522_11665"
     CDS             1710..1946
                     /gene="rpmB"
                     /locus_tag="PK522_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNC42433.1"
                     /translation="MARVCEVTGKKPMTGHHVSHANNKTKRRFLPNLQRRRFWVESEK
                     RWVTLRLTNAGLRTIDKNGIDAVLAKIRARGVDV"
     gene            1996..2151
                     /gene="rpmG"
                     /locus_tag="PK522_11670"
     CDS             1996..2151
                     /gene="rpmG"
                     /locus_tag="PK522_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNC42434.1"
                     /translation="MREKIKLESSAGTGHFYTTTKNKKTKPEKLEMKKFDPVARKHVT
                     YKETKLK"
     gene            complement(2201..>2421)
                     /locus_tag="PK522_11675"
     CDS             complement(2201..>2421)
                     /locus_tag="PK522_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNC42435.1"
                     /translation="SMRMSTQKLAQIAAKDPELQGLHRLGNNILILGGGLPITLNGEV
                     IGGIGVGGAPGIQFDEVCAEAGLKVFRN"
BASE COUNT          779 a          469 c          483 g          690 t
ORIGIN      
        1 aaaaaggaga aattagaaca gtaactgcaa gaggagatct tgctctagat gaacgcacta
       61 caattgaatt atttgagcaa tcccgtgatt ccgttgtatt catctccaca accaaattag
      121 tacgagatat ctggactcgc aatgtatttg ctgttccaag aggcaatgga tctggtttct
      181 tctgggacga tgcaggtcat attgtcacaa atttccatgt gatccaaggt gcgagtgaag
      241 caacagtcaa attagcggat gggcgtgatt ataaagcggc gctcattgga gtaagccctt
      301 cacatgatat tgctgtacta aaaattggtg tgggcttcaa gcgtcctaac cctattccaa
      361 taggaaccag ccataatctc aaagttgggc aaaaagtttt tgcaatcggc aatccctttg
      421 gactcgattg gactttaacg acaggtatca tttcagcgct agatcgttcc ctagcaagtg
      481 ataatggaac ggcaatcgaa cacttaatcc aaactgacgc tgcgattaat ccgggtaatt
      541 cgggaggccc tcttctcgac tcagccggta gattaatcgg tatcaatact gcgatttata
      601 gtcccagtgg cgcatcggcc gggatcggat ttgctgtacc cgtagatacg attatgcgag
      661 ttgttcctca gatcattgat aaagggaaat atattcggcc ctcgcttggt atcagtgttg
      721 atgaacgttt taatgatcga attgtacaat tactcaatat ccgtggtgta gtcgtattaa
      781 atgtagcgcc tggatccgct gctgaaaaag ctgggttaaa aggcgcggct attagcatag
      841 aaggtaatat taccccgcaa gatatcatca tcgctattaa tgggaagaaa gtagaatctg
      901 tctcaaaatt acttgccaga attgatgact ttaacgttgg agatgtcgta acccttacgg
      961 tattccgaaa tgaacaaacg cgagaagtcc atattacgct gcaacctggt gtttaaataa
     1021 tattcgcata atacaattgc ggtcgattat ggatttttct gattttaagc tcgatagcgc
     1081 tagcgtagat caatctcagg caatttgtga attattgaat tctgcttacc gtggaaccga
     1141 tggttggact actgaagtag atattatttc gggcaagcga gctgatcgta atgagattga
     1201 agcagcaatt aagctaccta atagctattt ttttgtagct aaccaatcaa accaactcgc
     1261 atcgtgtatc catatcacta aaaaggatga tcatgcccaa tttggttttt ttgctgttca
     1321 accatctctg caaggtaatg gattaggcac atatgtactc aaacaagcag aaatcttcgc
     1381 aaaaactcgc ttatattcaa aaaaactact catgattgtc gtaagtcagc gcactgaatt
     1441 gattgctttt tatgagcgaa ggggatatcg gcgaacaggc caagtcgaaa atttaccagc
     1501 aaatatcggc attcctaaac gaataaattt aaccattgat tatttagaaa agagtatcgc
     1561 tacgacttga ttaatttaaa aaataatctg atcagaattt aatatcaacc atcttcagtt
     1621 aattattctc actagataat taaaccgatt gtgtgaatct tttgatttag gatacaataa
     1681 aaaattatag aaaaatttga ggatttaaaa tggctcgtgt atgtgaagtt acaggtaaga
     1741 aaccaatgac tggtcatcat gtatctcatg caaacaataa aacgaaaagg cgattcttgc
     1801 ctaatttaca acgacgtcgc ttctgggttg aaagcgaaaa acgctgggtt acactacgcc
     1861 tgaccaacgc gggattacgg actatcgata aaaatggtat cgatgctgta cttgcaaaaa
     1921 taagggctcg tggggttgat gtctaattac ggttaaaatt agcaagctat ttcattaatt
     1981 tctggaaaat attatatgcg tgaaaaaatt aaattagaat cttctgctgg aacaggacat
     2041 ttctatacaa caacaaaaaa taagaaaacg aaacccgaga aattagaaat gaaaaaattc
     2101 gaccctgttg ctaggaagca tgttacttat aaagaaacca aacttaaata acaagaaacc
     2161 ctaaaaaatc tagggttcta ttaatctgat taccaaagct ttagttccga aacaccttta
     2221 gacctgcttc tgcacaaact tcatcaaact ggatcccagg agcaccgcca acacctattc
     2281 caccgattac ttctccattc agggtaatcg gtagcccgcc ccctagaata agaatattat
     2341 tacccaaacg atgcaatccc tgtaattcag ggtcttttgc agcaatttgc gctagctttt
     2401 gtgttgacat tctcatactt g
//
LOCUS       DAOQLJ010000252         2398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_27728_length_2398_cov_5.919334, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000252 DAOQLJ010000000
VERSION     DAOQLJ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27728_length_2398_cov_5.919334"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /locus_tag="PK522_11680"
     CDS             complement(<1..1480)
                     /locus_tag="PK522_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HNC42436.1"
                     /translation="MSIKKKGNKNQLRSLDPYLAREQQRYGSTALPSREFIQKVLEDQ
                     GTPIDGSTLCKLLNITAKETEFFNRRLTAMIREGQIIRNRKGDICVTNKLDLIKGIVQ
                     GHGDGFGFLIVDENGPDLYLSPREMQKVLNGDRVIARKTGKDRRGRLEGEIVEVLERA
                     NNELVGRFHTKQGVSFVTAENKRISQEILIPSNATLEAQSGQVVIVKIIQQPEAYSQP
                     IGHVIEILGNHDSPEMEIEIALRKFDLPYLFSEAIEKISAKFPKKVLKKDLTKRQDLR
                     HLPLVTIDGETARDFDDAVYCQMEAENFRLYVAIADVSHYVKDDDALDIEAFNRGNSV
                     YFPRRVIPMLPEVLSNGLCSLNPGVDRLCMVCEMLIEPHGVCLEYQFYPAVMHSHARF
                     TYNEVASILDDGDQTQAIQHPELHPQIQSLYKVYKALRKERKKRGAIDFETLETQMIF
                     NDQGKIAQIVPVKRNEAHSLIEECMLAANVCASDFLQRHEQPT"
     gene            1549..1633
                     /locus_tag="PK522_11685"
     tRNA            1549..1633
                     /locus_tag="PK522_11685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1583..1585,aa:Leu,seq:cag)
     gene            1893..2222
                     /locus_tag="PK522_11690"
     CDS             1893..2222
                     /locus_tag="PK522_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HNC42437.1"
                     /translation="MQWFYLTIAILSEVIATSALKASDGFTRWLPSIIVIVGYASAFY
                     FLSLTLRTIPLGVAYAIWSGVGVALITLIGWIVYQQSIGLGEIIGIGLIVSGVIVLNL
                     FSEGEAH"
BASE COUNT          666 a          512 c          489 g          731 t
ORIGIN      
        1 acgtcggctg ttcatgacgc tgcaaaaaat ctgaagcaca aacattggca gccagcatgc
       61 actcttcgat tagagaatgc gcttcatttc ttttgacagg aacgatttga gcgatcttac
      121 cctgatcatt gaatatcatt tgggtttcta gggtttcaaa atcaatagcc ccacgttttt
      181 ttcgttcttt gcgcaacgct ttgtatactt tgtagagcga ttgaatctgt ggatgaagct
      241 caggatgttg aatcgcttga gtttgatcgc catcgtctaa gatggaagca acctcattat
      301 aggtaaatct agcatgcgag tgcataacgg ctggataaaa ttgatactct aaacacacgc
      361 catgtggctc aatgagcatt tcacaaacca tacaaagccg atcaacccct ggatttaggg
      421 aacataaacc attggaaagt acttctggta acattggaat gacccgacgt ggaaaataaa
      481 ccgagttacc gcggttaaag gcttcgatat cgagtgcatc atcatcttta acatagtgac
      541 tcacatcagc gattgccaca taaagtcgaa aattctcagc ttccatttga caataaaccg
      601 cgtcatcgaa atcacgcgcc gtttccccgt cgatagtgac taaaggcaga tggcgaaggt
      661 cttgacgttt cgtcaaatct tttttaagca cttttttagg aaattttgca gagatttttt
      721 caattgcctc actgaacaga taaggcaagt caaatttccg cagtgcaatt tcaatctcca
      781 tttcaggaga atcgtgattc ccaaggattt caatcacatg gccgatgggc tgggaataag
      841 cctccggttg ctgaataatt tttacgatga caacttggcc tgattgggct tctaaagttg
      901 cgttggaagg aatcagaatt tcctggctaa ttcgtttatt ctcagccgtc acaaaactga
      961 caccctgttt ggtgtgaaat cgtccaacca gttcattatt ggcacgttcc aatacttcga
     1021 caatctcacc ttctaagcgg ccccgccgat ctttaccggt ttttcttgcg atgacacgat
     1081 caccgttcaa aactttctgc atttctcttg gactgagata caaatcaggt ccattttcat
     1141 caacgatgag aaaaccaaac ccatcgccat gaccttgtac aatccctttt attaaatcga
     1201 gcttattggt aacgcaaata tcacccttgc gattccgaat gatttgccct tcacggatca
     1261 tcgcggtgag tcgacgatta aagaattcag tttctttagc tgtgatatta agcaatttac
     1321 ataatgttga accatcaatc ggtgtcccct gatcttcgag tactttttga ataaactctc
     1381 ggctgggcaa agcggttgaa ccatagcgct gttgctcacg cgcaagatag ggatcaaggc
     1441 tacgtagctg atttttattg ccttttttct tgattgacaa aaaagtagtt tcctatagaa
     1501 tcggctggcc ttatgatttg atataaaggc caagtcataa caatcattgc ccagatggcg
     1561 gaattggtag acgcgcatgg ttcaggtcca tgtgccttcg ggtgtggagg ttcgagtcct
     1621 cttctgggca ccataatcga ttaataaccc attgaaaata aatataattt tattttcatc
     1681 ttaaatttat atatcaaaac ctattcccaa taagctgctg ctaaggttta ttgatatata
     1741 aatcaaaatt tggagaaaaa ccaaatttag cttatccctg atcttatatt agattcaagc
     1801 tgatgtatat tttaaccacc aaatgagagg taatatctgg cgattgcatc atttcagaca
     1861 aaaattctct aactcactac tcacaaaatc atatgcagtg gttctattta acaatagcaa
     1921 tcctatctga ggtcatcgca acctctgcac tcaaggcctc agatggattt actcgctggt
     1981 tgccttcgat aattgttatc gtcggctacg catcggcgtt ctattttctt tcactgacac
     2041 tgcgcactat tccgctcggt gtcgcctatg ctatttggtc tggtgttgga gtcgccttga
     2101 tcacgttgat tgggtggatc gtctatcagc aatctattgg tctaggagaa atcatcggta
     2161 tcggcctaat tgtttctggt gtaattgtac tcaacctttt ttcagaggga gaagcacatt
     2221 gagagctgcc agaaattgcc aaacctgtta gcaacaaata gaactgtccg gttttgtagc
     2281 aaataaattg tccgtattga atcgcattga gttgtcactt tattcattgc gcagttgttg
     2341 aggtgaatcg ccccgggttt tccggagata aaatgatttg agaggaggag gaagagat
//
LOCUS       DAOQLJ010000253         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_28392_length_2367_cov_7.100779, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000253 DAOQLJ010000000
VERSION     DAOQLJ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28392_length_2367_cov_7.100779"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PK522_11695"
     CDS             <1..791
                     /locus_tag="PK522_11695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110849.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNC42438.1"
                     /translation="RGQDALIVYDDLTKQAWAYRQISLLLRRPPGREAYPGDVFYLHS
                     RLLERAARVSAEYVEKYTNGAVKGKTGSLTALPIIETQAGDVTAFVPTNVISITDGQI
                     FLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGGIRLALAQYRELAAFAQFAS
                     DLDEATRKQLERGKMATELMKQPQFATQSVSEMALTLFALNKGYLDDVEVKRALAFES
                     ALRSYIRNQHAALIDKIESTKNLDEDTEKALIQAIEEFKKNGTY"
     gene            791..1678
                     /gene="atpG"
                     /locus_tag="PK522_11700"
     CDS             791..1678
                     /gene="atpG"
                     /locus_tag="PK522_11700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNC42439.1"
                     /translation="MAGSREIRNKIKSVKNTQKITRAMEMVAASKMRKAQERMRKARP
                     YGEKIRNVAAHMSRASTEYKHPFLIERDTVKRVGVIVVTSDKGLCGGLNTNILRRALN
                     EMKRWQAEGEEIEVCCIGNKGLGFMNRIGAKVISHVVSLGDTANIEKLIGAIKIVLDG
                     YTNDRFDRVYVFYNRFVNTMKQEPTMELLLPLSDDRFGNNKGDQETSPKKLWDYIYEP
                     EAKSVIDDVMIRYVEALIYQAVTENMASEQSARMVAMKAASDNAGNLINELTLIYNKS
                     RQAAITKELSEIVGGAAAV"
     gene            1711..>2367
                     /locus_tag="PK522_11705"
     CDS             1711..>2367
                     /locus_tag="PK522_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNC42440.1"
                     /translation="MSEGRIVQCIGAVIDVEFQKDGIPNVYDALTMEGSELTLEVQQQ
                     LGDGVVRTIALGSSDGLRRGMMVVNTKKQISVPVGTKTLGRIMDVLGRPIDEMGPVGA
                     EKTMPIHRVAPAFDELSASTELLETGIKVIDLICPFAKGGKVGLFGGAGVGKTVNMME
                     LIRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVALVYGQMNEPPGNRLRV
                     A"
BASE COUNT          716 a          416 c          593 g          642 t
ORIGIN      
        1 atagaggcca agatgcattg attgtttatg atgacttaac caaacaagct tgggcttata
       61 gacagatatc attattgcta cgccgtcctc caggtcgtga agcttatcca ggtgacgttt
      121 tctatttgca ttctcgattg ttggaacgcg cggcaagagt tagcgcagaa tacgtagaga
      181 aatatacgaa tggagctgta aaagggaaaa caggctcact tacagcgctt ccaattattg
      241 aaacgcaagc gggtgacgta acggcattcg ttccaaccaa tgtgatctcg attaccgatg
      301 gtcagatttt cttggaaaca gacttattca atgcggggat tcgtccagca atcaatgcag
      361 gtatctctgt ttctcgggta ggtggtgctg cgcaaacaaa agttatcaaa aagcttggcg
      421 gtggtattcg tttagcgctt gctcaatatc gtgaactagc agcgtttgcg caatttgctt
      481 ccgatcttga tgaagcaaca cgtaagcaac ttgagcgcgg caaaatggcg actgagctta
      541 tgaaacaacc tcaatttgca acacaaagcg tttcagagat ggcattgacg ctatttgctt
      601 tgaacaaagg atatctcgat gatgttgaag tcaaacgtgc attagcgttt gaatcagcat
      661 tacgaagcta tattcgtaat caacatgctg cattgatcga taagattgag agtaccaaaa
      721 atcttgatga agatactgag aaagcactga tacaagcgat cgaagaattt aagaaaaacg
      781 ggacttatta atggctggta gtagagaaat acgcaataag attaaaagcg taaaaaatac
      841 gcagaagatt acacgcgcca tggaaatggt ggccgcatca aaaatgcgta aagctcagga
      901 acggatgcgt aaggcacgtc cttatggtga aaaaatccgt aatgtggctg cccatatgag
      961 tcgtgctagt actgaatata agcatccttt tttaattgaa cgcgataccg tgaaacgtgt
     1021 tggtgttatc gtcgttactt cggataaagg tttgtgtggt ggactaaaca ccaatattct
     1081 gcgtagagcc ttaaatgaga tgaaacgatg gcaagcagag ggtgaagaaa tcgaagtatg
     1141 ctgcattggc aataaaggct tgggtttcat gaatcgcatt ggtgcaaaag ttatctctca
     1201 cgttgtcagc ctaggtgata ctgcaaatat tgaaaagttg attggtgcga ttaagattgt
     1261 tctagatggg tataccaatg atcggttcga tcgcgtctat gttttttata atcggtttgt
     1321 caacacgatg aagcaagagc cgacaatgga attattgttg ccactgtcag atgatcggtt
     1381 tggtaacaat aaaggtgatc aggaaacgag ccccaagaaa ctttgggatt atatctatga
     1441 acccgaggct aagtctgtta tcgatgatgt aatgattcgt tacgttgaag cactgatcta
     1501 tcaggcagta acagaaaata tggcatcaga acaatcagcg cgtatggtgg cgatgaaagc
     1561 cgcatcagat aatgctggca atttaatcaa tgaattgaca ttgatttaca acaaatcgcg
     1621 ccaagcggct attaccaaag aactatcaga aatcgtgggc ggtgcggccg cagtctaatt
     1681 tttttgtata tctattttta aggaacaata atgagcgaag gaagaattgt tcaatgtatc
     1741 ggtgcggtaa ttgacgtgga atttcagaaa gacggaattc ccaatgttta tgatgccctg
     1801 actatggaag gatcagagtt gacattggag gtccagcaac aattgggtga tggtgtcgta
     1861 agaaccattg ccttgggatc ttcggatgga ttgcgtcgtg gaatgatggt cgttaatacc
     1921 aaaaaacaaa tttcggtacc ggtcggaaca aaaacactcg gtcgcatcat ggatgtatta
     1981 ggtcggccta tcgatgagat gggaccagtc ggcgctgaaa aaacaatgcc gatacatcga
     2041 gtcgcacctg cttttgatga actttctgct tcaacagaac tccttgaaac gggtatcaag
     2101 gtgattgatt tgatctgtcc gtttgcaaaa ggcggtaagg ttggattatt tggtggtgca
     2161 ggtgttggca aaactgtaaa tatgatggag cttattcgta atattgcgat tgagcacagt
     2221 ggttattccg tatttgcggg agtgggcgaa agaacccgtg aagggaatga cttctatcat
     2281 gaaatgaagg attccaatgt attagataaa gttgcgttgg tgtatggtca aatgaatgag
     2341 ccacctggca atcgtctacg cgttgca
//
LOCUS       DAOQLJ010000254         2308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_29758_length_2308_cov_5.060808, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000254 DAOQLJ010000000
VERSION     DAOQLJ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29758_length_2308_cov_5.060808"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..576
                     /locus_tag="PK522_11710"
     CDS             112..576
                     /locus_tag="PK522_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNC42441.1"
                     /translation="MHYFVILIIASCIVSICVILHYEALRFLGSTLGAHVHKRIGVLL
                     VMFGLLIAHILEIWIFAIGYILVQHGIELGHIQGIEQGNLIDFVYYSSVVYTTVGFGD
                     LVPVGAIRMLTAAEGLTGLALITWSASFTFMAMQRFWPHPLANSHKKPGERD"
     gene            complement(634..1677)
                     /locus_tag="PK522_11715"
     CDS             complement(634..1677)
                     /locus_tag="PK522_11715"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42442.1"
                     /translation="MKSFWLIGLVLLTASCTHFAIEDCGSQVLGVNEARIRSSHFRML
                     LEDSSAAANRFLPTAVMSTLAYAEDRDCGKETAKITNQERESLEKILQTERWIEVTEI
                     EWVPACEDDVGLFYRVWKKEFDDTIQVVVAFRGTWELKDWFYGNMHWLTRFLPIEDQY
                     SSARKNMQKVFDYFGESKKPVQFFTTGHSLGGGLAQHILYSNPTKIIQAIAFDPSSVT
                     GFTDQTPEDQVSACECDSSVLNGETRIYRVYDAYEILANLRIFHKFFFPPERHIQEVR
                     FPNEASHSMKGLAFYLFHRANDKGTDIYANPWYSGKGNYDGTRSCTDAFANKQKKSCS
                     IKVTTDDWFKCPQ"
     gene            complement(1689..2156)
                     /locus_tag="PK522_11720"
     CDS             complement(1689..2156)
                     /locus_tag="PK522_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor family
                     protein"
                     /protein_id="HNC42443.1"
                     /translation="MQKLMSVAILGAILLMQACTNNEPPEPSPLPVACGGIQGKQCPN
                     ANQYCNLGMGNCSIADAQGTCADMPTICPELYQPVCGCDGKTYGNTCKAAAAGVSIDH
                     EGECESTPQACGGVAGLQCPQGQVCKDDPRDSCDPEKNGADCPGICEAPHVSQ"
BASE COUNT          596 a          546 c          502 g          664 t
ORIGIN      
        1 ttcctgcaca atcccctcat caattcagaa ggttagtttc tgattaattg acttgtctac
       61 tcaatgggta gtccctgtaa aattggctta tttctggctt aaccgacccc tatgcactat
      121 ttcgttattt tgatcatcgc gagctgcatc gtttcgattt gcgtcatctt gcactatgaa
      181 gcgctgcgct tcttgggttc aaccctgggc gcacatgttc ataagcgtat tggtgtgttg
      241 ctggtgatgt ttggattgct gattgcccat atcctcgaaa tttggatttt cgcgattggt
      301 tatatcctag tgcagcatgg cattgaattg ggccatatcc aaggcattga acagggtaat
      361 ctcatcgatt ttgtgtatta ctcgtcggtc gtttatacca cggttggatt cggtgatttg
      421 gtccccgtgg gcgcgattcg catgttaacg gctgcagaag gattgaccgg cctggcgttg
      481 atcacctggt cggcttcgtt tacattcatg gccatgcaac gcttctggcc gcatcccctg
      541 gcgaattcgc acaagaagcc aggagaacgg gattaatcta acgcagacct atcagaaaac
      601 aacagtccag cccaattgtt atgaaaattg tttctactgc ggacatttaa accaatcatc
      661 ggtagtcact ttgatgctac agctcttttt ttgtttattg gcaaaagcat cggtgcaaga
      721 tcgagttcca tcataattac ccttccctga ataccaggga ttagcatata tatcagtgcc
      781 tttgtcgttt gctcgatgaa ataaataaaa tgccaaaccc ttcatggaat gggatgcttc
      841 attgggaaag cgcacttctt gtatatggcg ttcgggtgga aagaaaaatt tatggaaaat
      901 gcgcagattc gctaatattt cgtaggcatc atagacccga taaatacgag tttctccatt
      961 caacactgaa gaatcgcatt cacacgctga aacttgatct tccggtgttt gatctgtaaa
     1021 accagtcact gaactcggat cgaatgcgat tgcttggatg attttggttg gattggaata
     1081 cagtatgtgc tgagcaagac cacccccgag agaatggcct gtcgtaaaaa actgcactgg
     1141 cttcttcgat tcaccaaaat aatcgaaaac tttctgcata tttttccggg cagaagaata
     1201 ttgatcttcg atcggtaaga accgtgtcaa ccaatgcatg ttgccataaa accagtcttt
     1261 aagctcccat gtaccgcgaa aagccacgac gacttgtatc gtgtcatcga attctttttt
     1321 ccacacgcgg taaaacaaac caacatcgtc ctcacaagct ggaacccatt cgatctcggt
     1381 tacttcgatc cagcgttcag tctgtaagat tttttcaagc gattctctct cttgattggt
     1441 aatctttgcg gtttcttttc cacaatcacg atcttctgca taagccaagg ttgacatcac
     1501 tgcggtggga agaaaccgat tcgctgcagc agatgagtct tcaagcaaca tcctgaaatg
     1561 cgatgagcga atccgcgcct cattgacgcc tagcacttgg cttccacagt cctcaatcgc
     1621 aaaatgagta caggatgctg taagcagaac taaaccaatc aaccagaaag atttcatttg
     1681 ttaacccgct attgactgac gtgaggtgct tcgcatatcc cagggcaatc tgcgccattt
     1741 ttctccggat cgcaactatc cctcggatca tctttacaga cctgaccttg aggacattgc
     1801 agcccagcaa ctcccccgca agcctgtggt gttgactcac attcaccctc gtgatcaatg
     1861 gatacacctg ccgctgcagc tttgcaagta ttaccatagg tttttccatc acagccacag
     1921 actggttgat agagttctgg gcaaatagta ggcatatcag cacaggttcc ttgtgcatcc
     1981 gctatgctgc aattacccat ccctaaatta caatactgat ttgcgttggg gcattgtttt
     2041 ccctggatgc caccacatgc cacaggaagt ggcgagggtt caggtggttc gttgttagta
     2101 caagcttgca tcagcaggat agcgcccaaa atagcgacac tcatgagttt ctgcattttt
     2161 gactcctttc aagttatctt actgcgaatt aaatgtgatt tgcagaccag ccagcagaag
     2221 caagctcagt catacaaaag atacctttca atttgaatcg ccccgggttt tccggagata
     2281 aaatgatttg agaggaggag gaagagat
//
LOCUS       DAOQLJ010000255         2277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_30524_length_2277_cov_4.069307, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000255 DAOQLJ010000000
VERSION     DAOQLJ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30524_length_2277_cov_4.069307"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="PK522_11725"
     CDS             complement(<1..1253)
                     /locus_tag="PK522_11725"
                     /inference="COORDINATES: protein motif:HMM:NF040129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HNC42444.1"
                     /translation="MADFAALTLSVDSTGIKQSINELDRLGAAGDKVERDISKSTGVI
                     GSSFESLKTAALGLASAYGAISAVRGLTDAAVDIQKFENTLVAATGSAGEAAKEMEFV
                     RKVSNDLGLDLRSTAEQYSKLAAAARDTKLEGKDTRDIFVGIAQAATALGSRADETAG
                     ALNAIQQIISKNVVSMEELRGQLGERLPGAMRIAQQAMGLTSEEFNKLVGSGSLTADV
                     LLPRMAEQFQKTFGQASQSAAQGLSGQINRFNNALFDLQVAIGRTGIIEFFTSATQSA
                     AGFVARSAEIVRLYSNDIKIAFAAVIDVGRGVINTFHAAALGVASIIEQVKALLQLDF
                     KKAISINPIDIIKKPFEESLTKTFDETVKNIANKPISLPEAKIPLAIDVKIPAALSGA
                     VKQAASKSKEISESERFIQSLKKEA"
     gene            complement(1591..2004)
                     /locus_tag="PK522_11730"
     CDS             complement(1591..2004)
                     /locus_tag="PK522_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42445.1"
                     /translation="MAGFGKLNITNICDTPVEFEYMGEDGVTKTGFYVSVLGQYSKKV
                     QDFILSLENKDRMRSFEERKRGKQESFKKAEQDMDDTIEAIAIRVAGWRGTVNGQEVP
                     EYSEENAIELIKVNPIIRSKVIEMSNDLANFTKSK"
     gene            complement(2034..>2277)
                     /locus_tag="PK522_11735"
     CDS             complement(2034..>2277)
                     /locus_tag="PK522_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42446.1"
                     /translation="DTYVQMFEDETEASVTMTLTTNNAANADFVSFTMPRIKVGGNSK
                     DDGQKGIVQTVPFTALLNPGTNNGDATTLMIQDSLA"
BASE COUNT          586 a          551 c          415 g          725 t
ORIGIN      
        1 tcagcttctt ttttcagtga ttgaataaag cgttctgatt ctgatatttc tttagatttg
       61 gaagccgcct gcttaaccgc gccgctcaat gccgctggta ttttaacatc gatagctagt
      121 ggaattttag cttcaggaag cgatattggc ttgtttgcta tgttcttgac tgtctcatca
      181 aaagtttttg ttaatgattc ttcaaacggc tttttgataa tatcaatcgg attgattgaa
      241 attgctttct taaaatctaa ctgtaataat gctttaactt gctcaatgat cgacgcaaca
      301 ccaagcgccg ccgcgtgaaa tgtattaata acaccacgtc ccacatctat gacagcagca
      361 aatgctatct tgatatcgtt ggaatataac cttactattt ctgcgcttct agcaacaaaa
      421 ccagccgctg attgagtagc actcgtgaaa aattctatta ttccagtcct gccaatagcc
      481 acttggagat caaacaaagc gttattaaat ctatttatct gtcctgaaag cccctgagct
      541 gcagattgcg aagcttgacc gaatgttttc tggaattgct ctgccatcct ggggagtaat
      601 acgtcagccg ttaaactgcc agaaccgaca agcttattaa actcttcaga cgtcaatccc
      661 atggcctgct gagcaatgcg cattgcgccg ggtaatctct caccaagttg accacgcaat
      721 tcttccatgc tgacgacatt tttggatatg atttgctgaa tagcattaag ggcccctgct
      781 gtttcgtcag ccctagagcc aagcgctgtt gcagcctgcg caatacctac aaaaatgtcc
      841 cttgtgtctt tgccttccag cttggtatct ctagccgccg ccgcaagttt agagtattgc
      901 tcagctgttg atctgagatc aagcccgaga tcgttagaaa ccttgcgcac aaactccatt
      961 tctttagctg cttcaccggc tgatccggta gccgctacaa gtgtattctc aaacttttga
     1021 atatcaacag ccgcatctgt aagcccgcgc actgcgctta ttgcgccata tgcactagca
     1081 agccctagcg cagcagtttt caatgattca aacgaactcc ctataacgcc tgtggattta
     1141 cttatatcac gctcaacctt gtcgccagcc gcgccaaggc ggtctagttc atttatagat
     1201 tgcttgatcc ccgtggaatc aactgatagt gtaagcgctg caaaatctgc catgtttttt
     1261 tctatttaat catttttaaa cgtaatacat ccaatttttt tatgatgttt acatcatgac
     1321 ttgatagact tctacccgtc agattcgccc atgcgcttat ctcttgataa gacaatggat
     1381 taaagccgaa ttctgttgag cccctcgctg agtctagctc acagaaccat tcccacagat
     1441 taggaaatat gccaattggt aattcggcaa gcatatcatg cttttttccg gtttgtctat
     1501 agtacgctag aagatgctgc cttaatgtac agccatcttc ctgttttttc tcaagctcaa
     1561 attctcgttt cgcataagca agtaggatat ctacttgctc tttgtaaaat ttgctagatc
     1621 attactcatc tcaattactt ttgacctgat aatcggattt actttaatta actcaatagc
     1681 attttcttct gaatattctg gcacttcctg gccgttaaca gtgccacgcc aacctgcaac
     1741 acgaatggcg atagcctcga tagtatcgtc catgtcctgt tcggcttttt tgaatgattc
     1801 ctgcttgcct cgcttgcgct cctcaaagct gcgcattctg tctttattct caagcgacaa
     1861 gataaaatcc tgaaccttct tactatattg accaagcacg gatacataaa aaccagtttt
     1921 tgttacccca tcttcgccca tgtattcgaa ctcaacaggg gtgtcacaaa tatttgtaat
     1981 atttagtttg ccaaaaccag ccatatatgt acccgtcctt ttttagttaa aaattaagct
     2041 aatgaatctt gaatcatcaa cgttgtagca tcgccgttgt tagttccagg gtttaacaat
     2101 gccgtgaatg gtacagtttg cacaatacct ttttgcccat cgtctttact gtttccacct
     2161 actttgatac gcggcatcgt aaacgataca aaatcagcgt tcgctgcgtt gttcgttgtc
     2221 aatgtcatag taactgacgc ttctgtttcg tcctcgaaca tctgaacata tgtatca
//
LOCUS       DAOQLJ010000256         2239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_31469_length_2239_cov_5.068223, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000256 DAOQLJ010000000
VERSION     DAOQLJ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31469_length_2239_cov_5.068223"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PK522_11740"
     CDS             <1..195
                     /locus_tag="PK522_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42447.1"
                     /translation="YAKYDVTACLPDGPFTPYRARANLTSGELVIPCVEVSGSEYNVV
                     MKQRGKSSNWEVDFVGTGCN"
     gene            complement(303..1226)
                     /locus_tag="PK522_11745"
     CDS             complement(303..1226)
                     /locus_tag="PK522_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNC42448.1"
                     /translation="MDRFENMSMFISVVEAGGISAAATRMNIAKSVISRRLKELEAYL
                     GVELFHRTTRRMNLTDSGRTFYQQSVRILADLIEAEHATSQFHGALKGSLKVALPLSF
                     GLMHLGPAINVFLEIHSEIRFDLDFNDRQVDLLAEGFDLAIRIANLPDSSLIARRIAP
                     INVILCASPAYLDRNGKPDSPAALAHHQCLVYNLTSNFEYWSLRDAEDQVTKVKITPY
                     LKASNGEFLRDAAVNGLGIALIPTFIAYQEIEKGALVPILDKYHYSSLAVYAIYPQTR
                     HLSQRVRAFIDFLIKRFEGSPYWDSYLKNHE"
     gene            1355..2224
                     /locus_tag="PK522_11750"
     CDS             1355..2224
                     /locus_tag="PK522_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNC42449.1"
                     /translation="MNKLSEASRIIPATSIREGAGVIVNRTIGTPMLRHYDPFLLLDH
                     ISSDQPQDYLAGFPAHPHRGFSTFTYMIDGHMTHHDSMGNHGDLDPGSAQWMKAASGV
                     IHSEMPKQQLGLMRGFQLWINLPAAHKMDRPEYREYSEKMFPVLRFPEYTVKVLVGHF
                     EGVNAPIIDPVTEVTYLDVQLQPDKAFRYDLPNDNSSFIYVFEGYGQLASQAVQRHSL
                     VILDASDNNPITFLAGNKGARFIVVSGKPIHEPIVRYGPFVMSTKEQIDQAMKDFQSN
                     QFVRERAWVNQNH"
BASE COUNT          651 a          450 c          484 g          654 t
ORIGIN      
        1 tatgctaaat atgatgtcac agcatgccta cctgatgggc cttttactcc gtatcgagca
       61 cgtgcgaatc taacgtcggg cgagcttgtg ataccgtgtg tcgaagtcag tggcagtgag
      121 tataatgtgg ttatgaaaca acgtggtaaa tcgtccaatt gggaagtgga ttttgttggt
      181 acagggtgca attaacgagc aaaatatttt taacaaagaa ttaccttgga ctgtcatcta
      241 gtcaatcgtc taaggtcatt caaccattta actaggttag tttctcctta gccttgggca
      301 atttattcat ggttttttaa ataactatcc caatagggag atccttcaaa ccgtttgatt
      361 agaaaatcaa taaatgctct gacacgttgc gaaaggtgtc gggtttgtgg atagattgca
      421 tagactgcca gtgaggaata gtggtattta tcgagaatcg gtactaaagc acccttttca
      481 atttcctggt acgcaatgaa tgtggggatt aatgcaatcc ccagcccatt aactgcagca
      541 tcacgaagaa attcaccatt gcttgctttt agataaggcg ttatttttac ttttgttact
      601 tgatcttccg catcgcgcag gctccaatat tcaaaattac tagtcaagtt atatacaaga
      661 cactggtgat gggcaagtgc tgccggtgag tctggtttac cgttccgatc aagataagct
      721 gggctggcac acaaaattac attgatgggc gcaattcgcc gtgcgatcaa acttgaatcc
      781 ggtaaattgg cgatacggat tgctaaatca aaaccctctg ccagcaaatc tacttgtcga
      841 tcattaaaat ctaaatcaaa tctaatctcc gagtggattt cgagaaatac attgatcgca
      901 ggccctaggt gcatcaaacc aaagctgagt ggtaacgcaa cttttaaact accctttaaa
      961 gcgccatgaa attgggatgt ggcatgttct gcttcgataa gatcagcaag aatacgaacc
     1021 gattgttggt aaaaagtgcg cccactatcc gtcagattca ttcgtcgggt agtacggtga
     1081 aacaattcaa ccccaaggta cgcttcgagt tctttcaaac gccggctgat tactgatttt
     1141 gcgatattca tccgagttgc tgcggcgctg atgccacctg cttctacaac gctgataaac
     1201 atactcatat tttcaaaacg atccataaaa tggttttatt gttgcttatt taagaaatat
     1261 taattctatt accgagtctt tattcttatt ttaacaacga taataatact tcctgacaat
     1321 attttataaa tcattgatat aaggagagcg cagcatgaat aaattaagtg aagcttctcg
     1381 tatcattcct gcaacgagca tacgagaagg ggcaggcgtg attgtaaatc gcacgatcgg
     1441 tacaccgatg ttgcgtcatt atgatccatt tctattgctg gatcatatta gtagcgatca
     1501 accccaggac taccttgctg gattcccagc tcatccgcat cgaggattta gtacctttac
     1561 ttatatgatt gatgggcata tgacgcatca tgacagtatg ggtaatcacg gagatcttga
     1621 tccaggatct gcacaatgga tgaaagcagc tagcggtgtt atccattcag aaatgccaaa
     1681 acagcaattg ggtttgatgc gcggttttca gctgtggatc aatttgccag cagcccataa
     1741 gatggatcgc ccagaatata gggagtatag cgagaagatg tttccggtat taaggtttcc
     1801 agaatacacg gtgaaagtac tcgttggcca ctttgaaggg gtcaatgcgc ctattatcga
     1861 ccccgttact gaggtaacgt atcttgatgt gcaattacaa ccggataaag cttttcgata
     1921 tgacctgcca aacgacaata gtagctttat ttatgttttt gaagggtatg gtcaacttgc
     1981 tagtcaagca gtacagcgcc attctcttgt gatattagat gcatcggata ataaccccat
     2041 tacatttctt gcaggaaata aaggagcacg ctttattgtg gtgagcggca aacccattca
     2101 cgaaccaatc gtacggtatg gtccttttgt gatgagcacc aaagaacaaa tcgatcaggc
     2161 aatgaaagat tttcagtcca atcagtttgt ccgtgaacgt gcttgggtaa accaaaatca
     2221 ttagatgatt aatattcta
//
LOCUS       DAOQLJ010000257         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_32857_length_2183_cov_5.093985, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000257 DAOQLJ010000000
VERSION     DAOQLJ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32857_length_2183_cov_5.093985"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PK522_11755"
     CDS             <1..814
                     /locus_tag="PK522_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNC42450.1"
                     /translation="FLNRDDLPNEEEQFAAYRFVAERMQGMPVTIRTFDLGADKNLKQ
                     DIHRAATNPALGLRAIRLSLSEPTMFLTQLRAILRASVYGNVRILIPMLSGDLEITQT
                     LQYIEQAKQSLRETQQPFDETIPIGGMIEIPAAALCVESFMRKLDFLSIGTNDLIQYT
                     LAIDRVDETVAHLYDPLHPAVLWLLSKIIHSANLENIPVSLCGEIAGDIQYTRLLLGM
                     GLKQFSMYPAQILTIKQEILKSHLPSIRPLTKKILKAEEPEKIRQLLSRLNN"
     gene            957..2084
                     /locus_tag="PK522_11760"
     CDS             957..2084
                     /locus_tag="PK522_11760"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNC42451.1"
                     /translation="MHDSDSIGIVTPQRLHFDSPLQLESGAILDNYELVYETYGELNA
                     ARSNAVLICHALSGNHHVAGVYADNPKNAGWWNNMIGPGKPIDTNRFFVIGVNNIGGC
                     HGSTGPTTINTKTNKPFGADFPMVTTIDWAETYVRLAQQLNIDQFAAVVGGSLGGMSA
                     MQLSLDAPEKVRHALVIAAAAKLTAQNIAFNDVARQAIITDPDYHGGDYYAQQTFPRR
                     GLRLARMLGHITYLSDDSMAEKFGRELRSGSLGYNFDVEFQIESYLRYQGDKFAEQFD
                     ANTYLLMTQALDYFDLAREFGDDPARAFAATACKFLVISFTTDWRFPPERSRELANAL
                     ISANKAVSYAEIESEYGHDAFLIPIERYKKVFAAYMQSIEV"
BASE COUNT          580 a          489 c          481 g          633 t
ORIGIN      
        1 ttttctaaac cgtgacgacc tccccaatga agaggagcaa tttgcagctt atcgctttgt
       61 tgctgagcgc atgcaaggga tgcccgtcac aatccgcaca tttgacttgg gcgctgacaa
      121 aaacctcaaa caggacattc atcgtgctgc aaccaatcct gcattgggat tgcgtgctat
      181 ccgcttgagt ttatcagagc ccaccatgtt tctgacgcaa ttacgcgcca ttctgagagc
      241 atctgtttat ggtaatgtgc gcattctgat tccaatgctt tcaggtgatt tggaaattac
      301 tcagactttg cagtacatcg aacaagccaa gcaatcgtta cgagaaactc agcaaccttt
      361 cgatgaaacc attccgatcg gtggcatgat tgaaatccct gcagctgcgc tctgtgtcga
      421 gtcatttatg cgcaaacttg attttttatc tatcggtacc aatgatctca tccaatatac
      481 acttgcgatt gatcgggtcg atgaaacggt tgcgcattta tatgaccctc tccaccccgc
      541 agtactttgg ctattatcta aaatcatcca ttcggcgaat ctggagaata tcccagtatc
      601 cctctgcggt gaaattgccg gtgatattca gtacacgcga ttattgctcg ggatgggctt
      661 gaaacaattc tcgatgtatc cagctcaaat tctcaccatt aaacaagaaa tattaaaaag
      721 ccatttaccc tcgattagac cgctgaccaa aaagattctg aaagcagaag agcctgaaaa
      781 aatacgccaa cttctgtctc gtttgaataa ttaaccattc gctttttttc catctaatgc
      841 aagtgagttt tatccgagaa gatcgcattt gctttgccaa acgctatatt attctcaaga
      901 aaatacgtat gattaacctt ttattgatac gctttcactc gtaaactgct cgacgtatgc
      961 atgattcaga ttcaattggg atagttaccc cgcaacgctt acattttgac tcccccctac
     1021 aactcgaaag tggcgctata ttggataact atgagcttgt ctatgaaact tacggagaac
     1081 tcaatgcagc acgatccaat gcagtgctga tttgtcatgc gctatcaggc aatcatcatg
     1141 ttgcaggcgt ttatgctgac aatccgaaaa atgctggttg gtggaataat atgattggcc
     1201 caggaaagcc cattgatacc aatcgtttct ttgtcatcgg tgtcaataac atcggaggct
     1261 gccatggttc aactggaccc actaccatta ataccaaaac aaataaacca tttggcgctg
     1321 atttcccaat ggttaccact atcgactggg ctgaaaccta tgtacgttta gcacagcaac
     1381 tcaatattga ccaatttgca gcagtcgtcg gtgggagtct aggcggtatg tccgctatgc
     1441 agttatcgtt ggatgcgcct gaaaaagtcc gacatgcttt ggttattgct gctgcagcga
     1501 aattaacggc acaaaatatc gcgtttaacg atgtagcacg gcaagcgatt attaccgatc
     1561 ctgattatca tggtggcgat tattatgcac aacaaacctt cccacgaaga ggattacgtc
     1621 tagcccgaat gttaggccat attacttatc tatcagatga ttcgatggct gaaaaatttg
     1681 gaagagaact tcggtctggt tcactgggct acaattttga tgtggaattt caaattgaat
     1741 cctacttgcg ctatcaaggt gataaattcg cggagcaatt tgatgccaat acctatttgt
     1801 tgatgacgca ggcgttggat tattttgatt tggcgcgcga gtttggtgat gatcctgcgc
     1861 gggcgtttgc ggctaccgcg tgcaagtttt tagtgatttc ttttacgaca gattggcgtt
     1921 ttccgccgga gcgttcgcgc gagttggcca atgccttaat cagcgccaat aaagccgtga
     1981 gctatgccga gattgaatcg gaatacggtc acgatgcgtt cttgatcccc attgagcgct
     2041 acaagaaagt gtttgctgct tatatgcaga gtattgaggt gtgagatgcg catagacctc
     2101 gatattttgc agaattggat agcgccgcaa tcgcgcgtgt tggatttagg ttgcggcagc
     2161 ggttctttat tatccaaatt agt
//
LOCUS       DAOQLJ010000258         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_33173_length_2172_cov_4.534719, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000258 DAOQLJ010000000
VERSION     DAOQLJ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33173_length_2172_cov_4.534719"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..2163)
                     /gene="recG"
                     /locus_tag="PK522_11765"
     CDS             complement(97..2163)
                     /gene="recG"
                     /locus_tag="PK522_11765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNC42452.1"
                     /translation="MITIHHSIFSRIEQKTQQKLAKLSIQNDTDLILHLPLRYEDETN
                     LYLINDVIDGNVFQVEGEVIHNEIIYRPRKQLIVQISDGSGTLLIRFFNFYPNQIKLY
                     SVGVRIRALGEVRRGFWGIEMVHPKCRIVQNETPLADTLTPVYSTTAGLSQPSIRKWI
                     QQALTRMDHQELLCEALPQEILSRYQLLEFKASLFVLHKPLAGISVTSLQERKHPAWR
                     RIKFDELLAQQLSMRLHYQQRRNNLAPVLTPKQSLTDQLLCALPFCLTAAQEKVLVEI
                     FHDLAAQQPMQRLLQGDVGSGKTIVSALAALRAIENGYQVAIMAPTEILAEQHHSRFI
                     SWLAPLDIEVAWLSGSQTKKQRERELARISTGKAMLAIGTHALFQDQVAFSRLGLVII
                     DEQHRFGVAQRLALKHKGNKKALVPHQLMMSATPIPRTLAMSYYADLDVSTIDQLPPG
                     RAPIVTKLIDNSRRAEVTQRVYEACNTGKQIYWVCPLIEESEALELKTAIETYEALSQ
                     HLSDIKVGLIHGRLNASEKSSIMTDFSQGNIQLLVATTVIEVGVDVPNASWMIIEHAE
                     RLGLSQLHQLRGRIGRGQEASLCILLYQKPLSKIARERLRIIFEHNDGFEIAQQDLRL
                     RGPGELLGTRQSGIPLLRFANLEEDKDLLEAARNVAEQLLNENPKVVEFYLTWWLASR
                     NVLLQA"
BASE COUNT          626 a          502 c          420 g          624 t
ORIGIN      
        1 tagcttttca ttaatagata ttcatggata cagtataaag aaaccaccga ttgcgatgat
       61 tcaattaacg tatagaaaaa ccattttaca cttaagttat gcttgtaaca ggacatttct
      121 tgatgccaac caccacgtta gataaaattc aacaacttta gggttttcat ttagcagttg
      181 ttcagcaaca ttgcgagccg cttctaataa atccttatct tcctctaaat tagcaaagcg
      241 caacaaagga ataccacttt gacgcgtgcc aagtaactcc ccaggccccc gcaatcggag
      301 atcttgctgc gcgatttcaa atccatcgtt atgttcaaaa ataatacgca accgttcccg
      361 tgcgattttg gataagggtt tctgatatag caatatacat aaacttgctt cctggccgcg
      421 gccaatacga ccgcgcagtt ggtgcaactg ggacaaaccc agccgctccg catgctcaat
      481 aatcatccaa gatgcattag gtacatcgac tcctacctca atgacggtgg ttgcaaccaa
      541 taattggatg ttaccttggc taaaatcagt catgatactc gatttttctg aagcattaag
      601 tctgccatga attaaaccta ccttgatatc tgagaggtgc tgactgagcg cctcataagt
      661 ttcaatggct gttttaagct ctaacgcctc tgactcttcg ataagtggac atacccaata
      721 gatttgtttt cccgtattac aagcctcata cacacgctgg gtgacttcag ctcttctact
      781 attatcaatc aattttgtga caatcggcgc gcgacccggt ggtaattgat cgatagtaga
      841 tacatcgaga tcagcgtaat aactcatcgc caaggttcgc ggtataggtg ttgcactcat
      901 cattaattgg tgaggaacca acgctttctt atttcccttg tgttttagcg ccagtcgctg
      961 cgcgacccca aaccgatgct gctcgtcaat gatcactagg cctagccgtg aaaatgctac
     1021 ttgatcctgg aacaaagcat gagtgcctat tgccaacatg gcttttcctg tcgaaattcg
     1081 ggcaagttct ctttcccgct gctttttagt ttgactccca gaaagccaag ccacttcaat
     1141 atcgagtggc gctagccaag aaataaatcg actgtgatgt tgctcggcta gaatctctgt
     1201 tggcgccatt atggccactt gataaccatt ctcgatggca cgcaatgcag ccaaagccga
     1261 aacaattgtt ttgccactac ccacatcacc ttgcaagagc cgttgcatgg gttgctgtgc
     1321 tgccagatcg tgaaaaatct caactaaaac tttttcttgc gcagcagtca gacaaaatgg
     1381 taatgcgcat aacaattgat cggtcaacga ttgttttggt gtcaatacag gtgcaagatt
     1441 attccttcgc tgctgataat gcaggcgcat agaaagctgt tgcgctaata attcatcaaa
     1501 ctttatcctg cgccaagcag gatgcttcct ttcttgcagt gaggtaacac taattcctgc
     1561 caatggctta tgcaacacaa aaaggctcgc tttgaattcc aataattgat agcgtgacaa
     1621 gatttcttgg ggaagagctt cacacaacaa ctcctgatgg tccattcgtg taagtgcttg
     1681 ttgaatccat ttgcgaatac tgggttgtga caaacctgct gttgtagaat aaactggcgt
     1741 taatgtatct gccaatggcg tttcattttg aacgatccga cacttgggat gaaccatctc
     1801 aattccccaa aaaccacgtc gaacttcacc taatgcacga atacgcactc caactgaata
     1861 gagtttgatt tgattagggt aaaaattaaa aaaacgaatc agcaaagtac cactgccatc
     1921 acttatctga acaataagtt gtttacgagg tcgataaata atctcattat gaatcacctc
     1981 tccttccacc tgaaaaacat tcccatcgat cacatcgttg atcagataaa gattcgtctc
     2041 atcttcataa cgaaggggca agtgtaaaat aagatccgta tcgttttgaa tacttaattt
     2101 tgctagtttc tgctgtgttt tctgttcaat ccgagaaaaa atagaatgat gaattgtaat
     2161 caaagcattc tt
//
LOCUS       DAOQLJ010000259         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_33252_length_2170_cov_3.942317, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000259 DAOQLJ010000000
VERSION     DAOQLJ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33252_length_2170_cov_3.942317"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PK522_11770"
     CDS             <1..869
                     /locus_tag="PK522_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNC42453.1"
                     /translation="GFVEVVRELEQRFPDPSAIEKESDKKAFAKLFGEYLRVENVLQN
                     YDEFASLKALQNIDMDDPAAVEAFKAKHYLSDDDLAALLSIKIPAERKIQDYRSTYND
                     IRDWLRREKSSAEKEKSTIDWDDVVFEIDLLKSQEINLDYILELIFENNKKVKDKAAL
                     VEDVRRVIRAGLGNRAKEGLLVDFINQTNLDQIGDKASVIDAFFTFAQAEQQREAQEL
                     ISDESLNAEAARRYITTSLKREFASDNGTELNAVLPKMSPLNPKYLTKKQSVFQKIAA
                     FVEKFKGVGGQV"
     gene            1179..1475
                     /locus_tag="PK522_11775"
     CDS             1179..1475
                     /locus_tag="PK522_11775"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC42454.1"
                     /translation="MILIDWNLIEKAIAEHYSPISDATGRPAYSGLLLLKMLLVGIWN
                     GGLSDESVEYMANSNLHVMRFLGLSLEDDVPDHSVLSRFRTRLTVAQAWDGLLT"
     gene            1634..>2170
                     /locus_tag="PK522_11780"
     CDS             1634..>2170
                     /locus_tag="PK522_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC42455.1"
                     /translation="MRVIEVGQSGVDTEARWVKKGDKFVFGYKQNTVVDDNGLVLAVE
                     TTAANCHDSKPLLDLLDKANIQPGTRIHADKAYSSQKHRDTLKSRGLKNGIQDKATKN
                     NPLKRRQLRRNRLITKACYVVERTFGSQARWFNAKTLRYCGLAKVRAWHMLVAIAYNL
                     KRLPKLFTESLMRQEKYVF"
BASE COUNT          661 a          447 c          538 g          524 t
ORIGIN      
        1 gtggctttgt ggaagtggta agggagttgg agcaacgctt tcccgacccc tctgccattg
       61 aaaaagagtc cgataaaaag gccttcgcga aactgtttgg cgaatatttg cgtgttgaga
      121 acgtgctgca aaactacgat gaattcgcca gcctgaaggc cttacagaac atcgacatgg
      181 atgacccggc ggcggttgag gcattcaagg ccaagcatta tttaagcgat gatgatttgg
      241 cagcgctgct atcgatcaag atacccgctg agcggaaaat tcaggactac cgttcaacct
      301 ataacgatat tcgcgactgg ttgcgtcgag aaaaatcttc cgctgaaaaa gagaaatcca
      361 cgattgattg ggatgacgtg gtctttgaga tagacctgct taagtcacaa gagatcaacc
      421 tagattacat tctggaacta atttttgaaa ataataagaa ggttaaagac aaagcggcac
      481 tggttgaaga cgtgcgccgg gtgattcgcg ccggcctagg aaaccgcgct aaagaaggtt
      541 tgctggttga ttttattaat caaacaaacc tagatcagat tggcgataag gccagtgtga
      601 ttgatgcttt cttcaccttt gcacaagcgg aacaacagcg tgaggcccaa gagctgatta
      661 gcgacgagag cctgaatgct gaggcagcga ggcgttatat caccacctca ctaaaacggg
      721 aatttgccag tgataatggc actgagctca atgccgtcct gcccaaaatg agtccgctta
      781 atccgaaata cctaactaaa aaacaaagcg ttttccaaaa aattgccgca tttgtggaga
      841 agttcaaagg cgtgggcggg caggtctaag aggatcacca tccatctggg aaaccttcaa
      901 accgatcgtg tgcggccacc ctcaaacctg cagcaaaccg caccagtgct catgagctcg
      961 ggagtgttga agaaacctac ctacatatat ccccagttct taatttcgcg actgtaaaat
     1021 tttgtaatcc aggtgagact tttgcacggt tataaattca accgatcatc cagatgctag
     1081 aattatgttt taaatcaata gcattgaagt aataagcatg agtttttcag aattagatgt
     1141 aacccggaga acccgcaaag ggaatttttt gaagcagatt gatattaata gattggaacc
     1201 tgatagagaa agcgattgcg gagcattatt ctccgatatc cgatgcgaca gggcgaccgg
     1261 cgtattctgg attgctgctg ttgaagatgc ttctggtagg gatctggaat ggtggtctaa
     1321 gcgatgaatc agtagaatac atggcgaatt cgaatctgca tgtgatgcga tttcttggac
     1381 tgtcactgga agatgatgtg ccggatcatt ccgtattatc acggttcaga actcggctta
     1441 ctgtggctca agcatgggat ggattattaa cgtaaatcaa ccagcaaata gtagttcatg
     1501 atattgcggt taaaaaaggc tgtcatgttg atgcgagcat tacacaaagt ccgcgtaaac
     1561 ctaaaccaag ccaacttatg aagtagtcag tgatcgggaa gaacgggatg atgaaccgaa
     1621 tgccaaggaa gcgatgcgcg tcattgaagt gggccaatca ggcgtagata ctgaagcacg
     1681 ctgggttaag aaaggcgata aattcgtatt tggctataag caaaatacgg tagtcgatga
     1741 taacgggctg gtattagcgg ttgaaacgac cgcagcgaat tgccatgaca gcaagccgtt
     1801 gctggatcta ctcgataaag ccaatattca accaggcacc cgtattcatg ccgataaagc
     1861 ctatagcagc cagaaacatc gcgatacgct gaaatcacgt ggccttaaaa acggcattca
     1921 agataaagcg acaaaaaata acccattgaa acgacggcag ttaaggcgca atcgtttaat
     1981 tacaaaagcc tgctatgttg tcgaacgaac cttcggcagc caagcacggt ggttcaatgc
     2041 caaaacttta cgttactgtg gtttggctaa agtacgcgcc tggcatatgt tggtagctat
     2101 agcgtataac ctaaaaaggt taccaaagtt gtttactgaa agcttgatga ggcaggaaaa
     2161 atacgtcttt
//
LOCUS       DAOQLJ010000260         2147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_33912_length_2147_cov_6.746654, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000260 DAOQLJ010000000
VERSION     DAOQLJ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33912_length_2147_cov_6.746654"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..379
                     /locus_tag="PK522_11785"
     CDS             92..379
                     /locus_tag="PK522_11785"
                     /inference="COORDINATES: protein motif:HMM:NF040598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpp/OprI family alanine-zipper lipoprotein"
                     /protein_id="HNC42456.1"
                     /translation="MNRNVKNALTIVALGATVALTGCATTAQFDELKAEVNAIKSDIS
                     SAKADAAAARAAADNANRVAAEARDIANQANARSIEAETRIDRAFKKSMYK"
     gene            complement(476..1273)
                     /gene="lgt"
                     /locus_tag="PK522_11790"
     CDS             complement(476..1273)
                     /gene="lgt"
                     /locus_tag="PK522_11790"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNC42457.1"
                     /translation="MLIHPQFNPVALSIGPLSIHWYGLMYLIGFAFFILLGRYRIKQF
                     NQSGITFTILDDALFYGVLGVILGGRVGHVLFYQFSYYLAHPLEIFAIWQGGMSFHGG
                     LIGVLLAMFFLARKYQLKWLAVTDFIAPLVPIGLGAGRIGNFINGELWGRPTEVTWGM
                     IFPHVDHIPRHPSQLYQFALEGVVLFILLWVYSNKPRSVGAVSGLFLIGYGLFRSFSE
                     FFRQPDDGFMGQLTLGISMGQWLSLPMIIGGIFLLIRSSRLHVPAHR"
     gene            1389..>2147
                     /locus_tag="PK522_11795"
     CDS             1389..>2147
                     /locus_tag="PK522_11795"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNC42458.1"
                     /translation="MPDNKRSQNITQGAKRAPNRAMLRAVGFSDGDFDKPIVGVANGY
                     STITPCNMGLNDLSLSAERALKRAGAMPQMFGTITVSDGISMGTEGMKYSLVSREVIA
                     DSIETAVQAESMDGVIAIGGCDKNMPGALIALARMNIPAIFVYGGTIKPGHYKGQDLT
                     IVSAFEAVGQFTAHKIDAKELLEVERHACPGAGSCGGMFTANTMSSAIEAMGMSLPYS
                     STMAAEDEEKKVSAAQSAEVLVQAIKKGIHPRDLI"
BASE COUNT          694 a          397 c          498 g          558 t
ORIGIN      
        1 ttattattta aagtataggg ttagcaagct tattattccc attgtatatg attgttaacc
       61 ctaatttatt caccttggag gagataataa tatgaataga aatgtaaaaa atgcattaac
      121 gattgttgca ttaggcgcaa cggtagcgtt gacaggatgt gcaacaaccg ctcaatttga
      181 tgagctaaaa gctgaagtca atgcaattaa atctgatatc agttctgcaa aagcagatgc
      241 agctgcggct cgtgcagcag cggacaatgc taatcgtgtt gcagcggagg cacgtgatat
      301 tgcaaatcaa gctaatgcgc gctctattga ggcagaaact cgaattgatc gtgcatttaa
      361 aaaatcaatg tataaataaa ttatttagat tgtttgataa aaaaggccct taccagggcc
      421 ttttgctttt ttacttgtta attcagagtg ttatcgttga attcctatgg ctaggttatc
      481 gatgagcggg aacatgaagg cgtgatgagc gtataagaag aaaaataccg cctataatca
      541 ttgggagcga taaccactgt cccatgctga ttcctaaggt taattgcccc ataaagccat
      601 cgtcgggttg cctaaaaaat tcagaaaatg agcggaataa tccgtagcca attaggaata
      661 gaccagaaac agcgccaact gaacgcggtt tattagaata gacccagagt aaaataaata
      721 gcacgactcc ttccagcgca aactgatata actgcgaagg gtgacgtggt atatggtcta
      781 catgaggaaa aatcattccc caggtaactt cagtcggcct tccccatagt tctccattga
      841 taaaatttcc gatacgaccc gcaccaaggc caattgggac aagtggcgca atgaaatcag
      901 taacagctag ccattttagt tgatattttc gagctaaaaa aaacattgca agaagaacgc
      961 caatgagtcc tccatgaaac gacattccac cttgccaaat cgcaaaaatt tctagagggt
     1021 gcgcaagata atagctgaat tggtaaaaca agacatgccc tacccgtcct ccgagtatga
     1081 cgcctaatac accataaaat agggcatcat cgagaatagt aaaggtaatc ccggattggt
     1141 tgaattgctt tattcgatac cgacccagca aaataaagaa tgcgaagcca atcagataca
     1201 tcaaaccata ccaatggata gataacgggc caatcgaaag cgcaacaggg ttaaactgtg
     1261 ggtggattaa catggttaag ttgttaattt acggtaaaat aagcattata aggcaattag
     1321 ttttcaatat cagttacgac tgagattaat cgttgatagg atatatttaa tagcatttag
     1381 gagagatcat gccagacaat aagcgaagtc agaacatcac tcagggagcc aagcgagctc
     1441 caaatcgagc tatgctaagg gctgttggat ttagtgatgg agattttgac aagccaattg
     1501 taggtgtggc aaatggatat tctacgatta ctccttgcaa catgggatta aatgacttat
     1561 ctcttagtgc agaacgagcg ctaaaaagag cgggggcaat gccacaaatg tttggcacca
     1621 ttactgtatc cgatggtatt tcgatgggaa ccgagggaat gaagtactct ctggtatctc
     1681 gcgaagtgat cgcagattca atcgaaacag ccgtgcaagc tgaaagtatg gatggggtta
     1741 ttgcgattgg tggctgcgat aagaatatgc ccggggcctt gattgcgctt gcaagaatga
     1801 atattcctgc tattttcgtc tatggcggaa cgataaaacc gggtcattat aaaggtcaag
     1861 atttaacaat cgtgagtgca tttgaagcgg tagggcagtt tactgcacat aaaattgatg
     1921 ccaaagagtt gttagaagtt gagcgccatg cttgcccagg tgcaggctca tgtggtggta
     1981 tgtttacagc aaatacgatg tcatctgcaa ttgaagcgat ggggatgagt ttgccttatt
     2041 cctcaacgat ggctgcagaa gatgaagaga aaaaggtaag tgctgcacag tctgcggaag
     2101 tgctcgttca agcaataaaa aaagggattc atcctcgtga tttaatt
//
LOCUS       DAOQLJ010000261         2146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_33948_length_2146_cov_5.251554, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000261 DAOQLJ010000000
VERSION     DAOQLJ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33948_length_2146_cov_5.251554"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PK522_11800"
     CDS             complement(<1..556)
                     /locus_tag="PK522_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HNC42459.1"
                     /translation="MSKQQNSVIQWFDDRFPLLSTWRAHLSEYYAPKNFNFWYYFGSL
                     AVLVLVNQLITGIFLTMSYKPDASMAFASVEYIMRDVDYGWLIRYMHSTGASMFFVVV
                     YLHMFRGMMYGSYRKPRELLWLIGMFIFFILMSLAFTGYILPWGQMSYWGAQVIVSMF
                     GAIPYIGDTLSQMILGDFTLSDAAL"
     gene            complement(557..1135)
                     /gene="petA"
                     /locus_tag="PK522_11805"
     CDS             complement(557..1135)
                     /gene="petA"
                     /locus_tag="PK522_11805"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNC42460.1"
                     /translation="MNSRRRFLVVTTSLAGGAACAGIATPFLLSMMPSERAKAAGAPV
                     EVDISKLEPGMLLMVEWRGRVVWILRRTEEMLGTLKKLEDQLADPASDKDQQPTYAKN
                     ETRSIKPEILVVEGVCTHLGCSPVFRKDIAPADLGADWLGGFFCPCHGSKFDLAGRVY
                     KSVPAPTNLAVPPHTYLSDNQLIIGSESKESV"
     gene            complement(1379..>2146)
                     /locus_tag="PK522_11810"
     CDS             complement(1379..>2146)
                     /locus_tag="PK522_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNC42461.1"
                     /translation="AFIFGWLGIASYFLWLLTNDVIYLNFARQAGIWFFLLPILLVVS
                     HRMIPFFSSRVLENYVMVRPYWILWVMLACSLGHGLLLLFNQSELTWVVDFVLASCAF
                     YLSIVWGFKRSFSVSLLAVLHISFAWLSIAMFLFALQSLMFWFDNERVMLLGLAPIHA
                     LVIGYFSSMVLGMASRVTLGHSGRPLVLDSFTWLLFLGFQWATFSRILPDMIPNLSEQ
                     YLYWYLVAGSIWLVCFTIWAFRFAPIYWRPRSDGKPG"
BASE COUNT          766 a          494 c          341 g          545 t
ORIGIN      
        1 ttagcgcagc atctgagagg gtaaagtcac ccaggatcat ttgtgagagc gtatcaccga
       61 tgtagggaat agcgccaaac atgctcacaa taacttgtgc gccccagtac gacatctgtc
      121 cccaaggtaa aatataaccc gtgaaggcca agctcattaa tataaagaaa ataaacatcc
      181 cgattaacca taagagttct ctcggtttgc gataagagcc atacatcatg cctcggaaca
      241 tatgcaagta tacaactaca aaaaacattg atgcgccagt tgaatgcata tagcgaatca
      301 accaaccata atcaacatct ctcataatgt attcaacaga agcaaatgcc atgcttgcat
      361 caggtttgta actcatcgtc aagaaaatac ctgtaatgag ttgattgaca agcactaata
      421 ccgctaatga accaaagtaa taccaaaaat taaaattctt gggagcataa tattcggata
      481 ggtgcgctct ccatgtggaa agcaacggaa aacgatcgtc aaaccactga atgacagaat
      541 tttgttgttt actcatttat acagattcct tactttcaga gccgataata agctgattat
      601 cacttaaata agtatgcggt ggcacagcta aattagttgg tgctggaacg cttttataga
      661 cacgaccagc caaatcaaat ttagaaccat ggcaagggca aaagaaccca cccaaccaat
      721 cagcccctag atccgcaggc gctatatctt tcctaaatac gggagagcaa cctaagtgag
      781 tacaaacacc ttcaactacc aatatctcag gttttataga ccgtgtttca tttttagcat
      841 acgttggttg ttgatctttg tctgaagcag gatcagccaa ttgatcttct aactttttca
      901 atgttcccaa catttcctca gtccgtctga gaatccatac tactcttcca cgccattcaa
      961 ccattaacag cataccaggt tcaagtttac ttatatcaac ttctactgga gctccagcgg
     1021 ccttggcacg ttcactcggc atcatactca gcaaaaacgg tgtagcaatc cctgcacatg
     1081 cggcaccacc tgctaaagat gtcgtcacta ccaaaaatcg tcgtcgacta ttcatttcac
     1141 ctctaccatc aaccaaattt taagaattaa tttatagcta cctttaccca aaacaaccaa
     1201 tttattcttc tgattttata gactatttat atattacgaa ttaatttaag aacttttatc
     1261 ttatttctta ttcaaattaa tcttacaccg taaccagggt attgtaacat gttaacccta
     1321 aacacaatat aggtaagcta tacaatttca cacacgatta attaatttaa ctttacgatt
     1381 aaccaggttt accatcactt cgaggcctcc aataaatagg agcaaaacga aaggcccaga
     1441 tagtgaaaca aactagccaa atgcttccag cgactaaata ccagtataga tactgttcag
     1501 ataaattagg gatcatatct ggtaatattc tagaaaaagt tgcccactga aacccaagaa
     1561 aaagcaacca agtaaacgaa tctaatacaa ggggtcgacc tgagtgacct aatgttactc
     1621 ttgaagccat tccgagaacc atactcgaaa aatacccgat cactaaggca tgaatgggtg
     1681 ccaaacccaa taacatcacc cgttcgttat caaaccaaaa catcaaactt tgtagtgcaa
     1741 ataaaaacat tgcaatactt agccaagcaa acgaaatatg caatactgct aacaagctga
     1801 cgctaaaact acgcttaaat ccccaaacaa tcgacagata gaaagcacaa ctagctaaaa
     1861 cgaaatcaac tacccaggtc aattcggatt gattaaataa cagcagcaaa ccatgcccta
     1921 aggaacatgc aagcattacc cataaaatcc aataaggcct gaccatcaca taattttcta
     1981 gcactctact cgagaagaaa ggaatcattc ggtgtgaaac cactaatagt atcggtagta
     2041 agaaaaacca aattccagct tgccgtgcaa aattaaggta tataacatca tttgttaaaa
     2101 gccagagaaa atacgatgct atccctaacc atccaaagat aaatgc
//
LOCUS       DAOQLJ010000262         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_34065_length_2142_cov_6.901294, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000262 DAOQLJ010000000
VERSION     DAOQLJ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34065_length_2142_cov_6.901294"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="PK522_11815"
     CDS             <1..1638
                     /locus_tag="PK522_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNC42462.1"
                     /translation="IWSQQDTLMEDIERIEVIRGPGATVWGANAVNGVINIITKKAID
                     TQGTMITAGGGSFEQGFIGMRYGGKINEATPFRIYAKGFSRDNTHSLAGSNLQDNWRS
                     ARAGFRLDHTRGIDQLTLQGDVFINRIGDSLLQPTLISPFSRLDVVDTTEKGGNIRFR
                     WDRNFSDQSSIMLQTYYDRVQNQLTPWTKYAESFDIDFQHRLPILKSHDVTWGLNYRL
                     YHNKVAETELTQFNPAQKTNHNFSAFIRDEVTVIPEKLSLSIGTRLDHNDFTGLEIQP
                     SGRLMLTPNEYHSIWLSVSRAVRIPSRGENDVTIAGRTLQSLPGLPGLPIPVLMAVQG
                     SNQFASEKLIAYEMGYRHQVSANASIDLATFFNDYSQLRDFRFGQMMPGTGVIPHWIL
                     PVIFSNTTSAYSYGFESAIDWRPFKKWRLQSSYSYLTIHTNSNPEFRQLDSSSAGADK
                     ANPNHQLSIRSHYDFSEQLQLNLWLRYVSALTFFNIPSYVTMDAKLSWKPVRNTELFV
                     VGQNLFSKNHRESQSDFIPSIPALIPRGIYVGIERRF"
     gene            1734..2135
                     /locus_tag="PK522_11820"
     CDS             1734..2135
                     /locus_tag="PK522_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42463.1"
                     /translation="MKLDSTIIRWILLVPATWVAWYIAVFLGIAILTAGDAFCPSEQI
                     VSGWCHANWYPFFLQSVMVFSSSVSAIFVVVAGYYMAPSHRSFVAKLIFITGSAVAIF
                     MAIETQEYLALIGALLCGLGSLLIVIKDRAQ"
BASE COUNT          578 a          444 c          483 g          637 t
ORIGIN      
        1 atctggagtc aacaagacac tctcatggag gatattgagc gaatcgaagt gattcgtggt
       61 ccgggcgcaa ccgtttgggg ggctaatgct gttaatggtg ttattaacat catcactaaa
      121 aaggccattg atacgcaagg caccatgatt actgcagggg gtggtagctt tgagcagggg
      181 tttattggca tgcggtacgg aggaaaaatc aacgaagcga cgccgttccg gatatacgct
      241 aaaggattta gtcgcgataa tacgcattca ctggctggaa gtaatctaca ggataactgg
      301 cgttcagcca gggctggatt tcgcttggac catacccgtg gcatcgatca attgacgttg
      361 cagggtgatg tttttattaa tcgaattggc gatagtttat tacagcctac gttaatatct
      421 ccctttagtc ggcttgatgt tgttgatacg acagaaaaag ggggaaatat tcgtttccgg
      481 tgggatcgaa atttttctga tcaatcatcg atcatgctgc aaacctatta tgatcgcgtc
      541 caaaatcaac tgacgccatg gacaaaatat gctgaaagct ttgatatcga ttttcaacat
      601 cgattgccga tactaaaaag ccacgatgtt acttgggggc taaattatcg actgtatcat
      661 aataaagtcg cggaaacgga actcacgcaa tttaaccctg cacaaaaaac caatcataac
      721 tttagcgctt ttattcgtga tgaagtgacg gtaatcccag agaaactgtc attgtcaatt
      781 ggtacacgcc ttgatcacaa tgattttacc ggattagaga tacaaccgag tggccgttta
      841 atgttgacgc ctaacgagta tcactcgatt tggttatcgg tatcgcgtgc agtaagaatc
      901 ccgtcacgcg gtgaaaatga tgtaaccatc gctggccgga cattacaatc gttgccagga
      961 ttgcctggat tacccattcc agtactcatg gcagtacaag ggagtaatca gttcgcttca
     1021 gaaaaactaa ttgcttatga aatgggttat cgtcaccagg tgtcagcaaa tgcatcgatt
     1081 gatttggcaa ccttttttaa tgattatagc caattaaggg attttcgctt tggtcaaatg
     1141 atgcctggaa caggtgtgat accgcattgg atattaccgg taatattcag taacacgact
     1201 tcagcctact cgtatggttt tgaatctgca attgactggc gcccgttcaa aaaatggcgc
     1261 ttacaaagca gttactccta tttaactatt cacacgaatt cgaatcctga gtttcgacaa
     1321 ctagactcat cctctgcggg tgctgataaa gccaacccaa accatcagct ctcgatccga
     1381 tcgcattacg atttttcgga acagttgcaa ttaaatttgt ggttgcgcta tgtcagtgct
     1441 ttgactttct tcaatattcc cagctacgta acgatggatg caaaactatc ctggaaaccc
     1501 gtgaggaata cagagctttt tgttgtgggc caaaatttgt ttagtaagaa tcatcgtgaa
     1561 tcacaatcag atttcatccc ttctattcca gcgctgattc cacggggtat atatgtgggc
     1621 attgaaagac gtttctaaaa aagcgatgaa tctaaaattc tctatctgct gagttgtgag
     1681 tcagatcatt tttctgctgc gcgagtggtt tgctaattac taacctaaac ataatgaagc
     1741 ttgatagcac tattatccgc tggatccttc tggtaccagc aacttgggta gcttggtata
     1801 ttgctgtttt cttaggcatc gcaatcctta cggctggtga cgcattttgt ccctctgagc
     1861 aaatcgtgtc aggttggtgc catgccaatt ggtatccctt ctttcttcaa tcagtaatgg
     1921 ttttttcttc gtcagtatca gctatttttg tcgtcgtggc tggttattac atggcaccat
     1981 cacaccgatc tttcgttgca aagttaattt ttatcacagg ttcggcagtt gctatcttca
     2041 tggcgattga aacccaagaa tatctggcgc ttatcggcgc acttctatgt ggattgggct
     2101 cgctattgat tgtgataaaa gacagggcgc agtgagtaac ga
//
LOCUS       DAOQLJ010000263         2127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_34470_length_2127_cov_6.340251, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000263 DAOQLJ010000000
VERSION     DAOQLJ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34470_length_2127_cov_6.340251"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PK522_11825"
     CDS             <1..598
                     /locus_tag="PK522_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNC42464.1"
                     /translation="FSTELAHHNIAYQWFRRDGVLAFLPLPDKRVSIVWSTHETHANE
                     LRNVSDEEFSQRVSEASNRVLGRLQLITQPAGFPLNFVHVSKLVKPRLVLIGDAAHGI
                     HPLAGQGVNLGLRDVKALSEILKKDEIYYDYGDYILLLRYERARKADITAMETVTDGL
                     HKLFNSQDPIIRRFRNLGLTMTNRLPFIKSKLMQHALN"
     gene            652..1371
                     /locus_tag="PK522_11830"
     CDS             652..1371
                     /locus_tag="PK522_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HNC42465.1"
                     /translation="MMRLRSLMILLLLSCLISQALADETQLRKLLEDHFPGTEIESLR
                     KTPFAGLYEVVVGGEVLYTDDQAKYFFMGHMIDTQSRVSLTSERMQQIRDARRIPIDT
                     LPLEHSIKVVKGKGERTLIVYSDPNCPFCKKLEKELVDIKNITVHTLLYPILNGSMPT
                     ATSIWCASDRLKAWDDFMLHGKAPASKECETPIQALLQSGQKYRVTGTPTLIFADGSV
                     VSGMIPAAEIEKRLEQAKKPK"
     gene            1777..>2127
                     /locus_tag="PK522_11835"
     CDS             1777..>2127
                     /locus_tag="PK522_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNC42466.1"
                     /translation="MPHPLRNIAIIAHVDHGKTTMVDKLLHQAGTFAAHQTIAERVMD
                     SNDIERERGITIFSKNCAVDYEGVHINIVDTPGHADFGGEVERVLSMVDGVLLLVDAV
                     EGPMPQTRFVTKKAL"
BASE COUNT          626 a          453 c          451 g          597 t
ORIGIN      
        1 ttttagcact gaacttgcac atcataatat tgcctatcag tggttcagaa gagacggggt
       61 tctcgcattt ctaccgcttc ctgataagcg cgtgtcgata gtctggtcta ctcatgaaac
      121 ccacgcaaat gaattacgta acgtgtcaga tgaagaattt tcccagcgag tttcagaggc
      181 ttccaatcgt gttttgggta ggttacaact catcacacag cctgcgggtt ttcctttaaa
      241 ttttgttcat gtaagcaagc tcgtgaaacc gagattagtg ctgattggtg atgctgcaca
      301 cggcattcat ccactcgctg gccaaggggt taatctgggt ttgcgtgatg taaaagcatt
      361 gtcggaaatc ttgaagaaag atgagatcta ttacgactat ggtgattata ttctactcct
      421 ccgatatgag cgggcacgca aagcagatat cacagcgatg gaaacagtca cagacggcct
      481 acataaacta tttaacagtc aggacccaat aatcaggcgt tttcgtaatc ttggccttac
      541 gatgaccaat cgcctacctt ttatcaaaag caaactcatg caacacgcac tcaattaagc
      601 ttgtgctctg attattttcc cattcttcca taataaatag gagttattac catgatgcgg
      661 ttacgatcat tgatgatatt attgctgcta agttgtttga tcagtcaggc attagcagat
      721 gaaacgcagc ttaggaaatt actcgaagat cattttccag gaacagaaat cgaaagctta
      781 cgtaaaacac cttttgcagg cctatatgaa gtagttgtcg gtggagaagt tctctatacc
      841 gatgatcagg caaaatattt tttcatgggc cacatgattg atacgcaatc gcgagttagc
      901 ctaacaagcg agcgtatgca gcaaatcaga gatgcgcgac gaattccaat tgacacgctc
      961 ccccttgaac attcgatcaa ggtcgtaaaa ggaaaaggtg agcgtacttt gattgtgtat
     1021 tccgatccca attgcccctt ctgcaaaaag cttgaaaaag agctggtaga tatcaaaaac
     1081 attaccgtcc atacgctgct ttatccgatc ttaaatggct cgatgccaac ggccacatcg
     1141 atttggtgcg catcagatcg gttgaaagct tgggatgatt ttatgttgca tggtaaagca
     1201 ccagccagca aagaatgtga aacaccgatt caggctttgc tccaatcggg ccagaaatat
     1261 cgagtgactg gcacgcctac gctcattttt gctgatggat cggtggtatc aggaatgatt
     1321 ccagcagcag aaatcgagaa gcgcctagag caggctaaga aaccaaaata agcaaaatat
     1381 acaaccagaa ataatcctca cctgaaatcg attatttctg gttaagagaa ataaccctat
     1441 ctttccgtaa tcattcgatg attggtaaag cgcttgggaa ggttagctta aaactacaat
     1501 aacacagtgt ttggttgtct attcaatttc tacgcttctt agttattgaa ttaaatttac
     1561 ttccaccaga aatctgcttt tcgggtgcgg caggttacat cctatttcca aaaattgggc
     1621 ccaacacctc aacactattt aaaaagatta aaggataaaa tttaatatcg ataacgagat
     1681 tgagaaataa ccaagctagc tatgtaattg atctactgtt ataattcgcc cccttcacgt
     1741 ataaatcatt ctaattaaca ttcagagatt tttgacatgc cacatccact tcggaatatt
     1801 gcgattattg cccatgtcga tcatggcaaa actaccatgg ttgataaact gctccatcag
     1861 gcgggaacct ttgccgctca ccaaaccatt gctgagcgtg tcatggattc caatgatatt
     1921 gagcgggagc gtggcattac cattttttcc aaaaactgtg ctgttgatta cgaaggcgtg
     1981 catatcaata tcgtcgatac gcccggtcat gctgactttg gtggtgaagt cgagcgcgtt
     2041 ctttcaatgg tggatggtgt tttgctgctc gtcgatgcag tagaaggccc gatgccgcaa
     2101 acacgttttg taacaaaaaa ggcactt
//
LOCUS       DAOQLJ010000264         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_34903_length_2113_cov_5.223032, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000264 DAOQLJ010000000
VERSION     DAOQLJ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34903_length_2113_cov_5.223032"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1575)
                     /locus_tag="PK522_11840"
     CDS             complement(<1..1575)
                     /locus_tag="PK522_11840"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNC42467.1"
                     /translation="MNPLLDFTSLPRFMDIKPEHVLPAIETLLQQCRSVADQARTAAT
                     ATWNSFIQPLVDANERLSRAWGQVSHLNSVMNSPELREVYNANLPKVTQYFAELSQDQ
                     SLFEKFKQLRNSPEFNDLSNARKKIIENQLRDFRLGGAELTTDNKKRFLAIQEELSAL
                     SAKFSDNLLDATNDFYLIIAQQDELSGIPEDALLAAKEAAEKAPNPSKGWKFTLHAPS
                     YLPVMQYADNRDLREKMYRAYQTRASEIDSLVHGTSNWDNMPLINKILKLRKEEANLL
                     GFDCYAEVSLAPKMAESPRQVLDFLHELAAKAKPYAQRDLEELKVFARTKLGLEKLES
                     WDLAYASEKLRVERYAFSDQEVKQYFPENKVLPGMFRLVETLYGITISEASLSRPIQR
                     WHPDVKFFDIHDAKGQLIGQFYLDLYARPSKRGGAWMDDAMTRRRIEQENRQSSIQIP
                     VAYLTCNFSAPVNKNGQARPALFTHDEVIVLFHEFGHGLHHLLTQVEDLAVSGINGVE
                     WDAVELPSQFMENFCWE"
     gene            complement(1637..>2113)
                     /locus_tag="PK522_11845"
     CDS             complement(1637..>2113)
                     /locus_tag="PK522_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC42468.1"
                     /translation="IGFFNTNCHLIPPLRNLQDRDSLRAGLIDGTIDAICSDHAPIDD
                     DAKLLPFGESEAGASGLELLLSLTLKWTDSMNLSLIDGLKVITQTPASILAIDAGHLS
                     LGAAADICIFDPEAYWKVTPSTLKSQGKNTPFMGMELPGSVKYTLVGGNIIYQRSN"
BASE COUNT          588 a          435 c          474 g          616 t
ORIGIN      
        1 ttcccagcaa aaattttcca taaactggct gggcaactca acggcatccc attcaacccc
       61 attgatgccg gacactgcta aatcttcaac ttgagtcagt aaatggtgta ggccatgacc
      121 aaattcatga aatagtacga tcacttcatc gtgggtaaac aatgcgggcc gtgcctggcc
      181 atttttattg acgggtgccg agaaattgca agtcaaataa gcgaccggaa tctgtataga
      241 tgattgccta ttttcttgct cgatgcgacg ccgagtcatc gcatcatcca tccatgctcc
      301 accgcgttta ctggggcgtg cgtagagatc aaggtaaaat tgaccgatca attgaccctt
      361 tgcatcatga atatcaaaga atttcacatc gggatgccag cgctgaatgg ggcgtgaaag
      421 gcttgcttca gaaatggtaa ttccatacag tgtttcaact aatcgaaaca ttccagggag
      481 gactttgttt tcagggaaat attgtttaac ttcttgatct gagaaggcat agcgttctac
      541 acgaagtttt tcacttgcat atgctaaatc ccatgattct agcttttcta acccgagttt
      601 ggtgcgggca aatactttaa gctcttctag atctcgttga gcgtaaggct ttgctttagc
      661 agcaagttca tgtaagaaat cgagtacttg tcgaggtgac tcagccattt tgggcgctaa
      721 agatacttct gcataacaat cgaagccgag taaattagct tcttctttgc gcagctttag
      781 aattttgttg atgagtggca tattgtccca attcgatgtg ccatgcacta ggctatcgat
      841 ttcgcttgcg cgagtttggt aggcgcgata catcttttcg cgtaagtcgc gattatcagc
      901 atattgcatg accggtagat acgaaggggc atgtaaagta aacttccaac ccttggatgg
      961 gttaggtgct ttttcagcag cttctttggc tgctaaaagc gcatcttctg ggataccaga
     1021 aagctcatct tgctgagcaa taatgaggta gaaatcattg gtcgcatcaa ggagattgtc
     1081 gctaaacttc gcagataaag cagataactc ttcttgaatc gcaagaaagc gttttttgtt
     1141 atcggtagtc aattctgcgc cacccaatct aaaatcgcgt aactgatttt caataatttt
     1201 cttgcgagca ttgcttagat cattaaattc agggctattg cgtaattgct taaatttttc
     1261 aaataacgat tggtcttgtg ataattccgc aaaatactgc gttactttgg gtaagttggc
     1321 attgtagact tcacgcagtt ctgggctatt cattactgaa tttaaatgtg atacctgtcc
     1381 ccaagctctg gatagtcgtt cgtttgcatc tacgaggggt tgaataaagc tattccaagt
     1441 agcagtcgct gcggtgcgag cttgatctgc aacggatcga cattgctgta gtaatgtttc
     1501 aatagcaggc aaaacatgtt cgggtttaat atccatgaag cgaggtaggc tagtaaaatc
     1561 aagtaaggga ttcatagcaa gtgcacttaa agaaaatgat tgaaagaaat aattgagtcg
     1621 aaaccgaccg ggtagtttag ttactacgct gataaatgat attaccaccg actaaagtat
     1681 atttaacaga accaggaagc tccatgccca taaaaggagt gtttttacct tggctcttta
     1741 gggttgatgg cgttactttc cagtaggctt cagggtcaaa gatacaaata tcggctgctg
     1801 cacccaggct caagtgccca gcatcaattg ccaagatgct agctggtgtc tgggtaatga
     1861 cttttagtcc atcaattaat gagagattca ttgaatcggt ccattttagc gtcaaagata
     1921 gtagcaactc taatccacta gcacctgctt ccgattcacc gaaaggcagc aatttagcgt
     1981 catcatcaat gggtgcatgg tcggaacata ttgcatcgat cgtgccatcg atcaaacctg
     2041 ctctcaacga atctcgatcc tgcaagtttc tgagtggcgg tataaggtgg caattggtat
     2101 taaagaaacc aat
//
LOCUS       DAOQLJ010000265         2096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_35440_length_2096_cov_6.772171, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000265 DAOQLJ010000000
VERSION     DAOQLJ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35440_length_2096_cov_6.772171"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PK522_11850"
     CDS             <1..261
                     /locus_tag="PK522_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42469.1"
                     /translation="SLPPPLKSFYLRKTRGDSIAESIDISAYALKRLVNHKMSNGVTA
                     GYIVSDVERLRKPMQQISSQLLRYFRIDDEKRVLSFPAYQST"
     gene            286..552
                     /locus_tag="PK522_11855"
     CDS             286..552
                     /locus_tag="PK522_11855"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNC42470.1"
                     /translation="MCKSYNLLIRSQAKKKLQSLPRVDRFRIAEKLEQLGRDPDNSSL
                     DIKKLEGEPYFRLRVGNWRVIFERHDVVRIITIEKIKPRGDAYK"
     gene            549..881
                     /locus_tag="PK522_11860"
     CDS             549..881
                     /locus_tag="PK522_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC42471.1"
                     /translation="MTLQKIKSIHGKDEYVLLPIAVYRVLKDQIEKELATCEANRDDE
                     QSYEPFVLEDYVDNPVALARIKAGITQEQLAQRLGVSQAYVSQIERRNNVTNKVLERV
                     YTAIQGTT"
BASE COUNT          708 a          407 c          384 g          597 t
ORIGIN      
        1 tctcttcctc ctcctctcaa atcattttat ctccggaaaa cccggggcga ttcaattgct
       61 gaaagcatcg acatatccgc ttacgcactc aaacgattgg ttaatcataa gatgtcgaat
      121 ggtgtgacgg ctggttatat cgtcagcgat gttgaacgat tgcgtaaacc catgcagcaa
      181 atatcctctc aattacttcg atactttaga atcgatgatg aaaaaagagt attgtcattt
      241 ccagcgtatc aaagcacttg aatataatat tttaaaactt ataatgtgtg taaatcttat
      301 aatctactaa tacgatccca agcaaaaaag aaattacaaa gcttgccaag agtagaccga
      361 tttaggattg ctgagaaatt agaacagcta ggtcgggatc cagataatag ttcacttgat
      421 atcaaaaaac ttgaaggtga gccttatttc cgtctgagag ttggtaattg gcgtgttatt
      481 tttgaacggc atgatgtcgt tagaattatt acgattgaga aaattaaacc gcgtggagat
      541 gcttacaaat gacactgcaa aaaatcaaat ccatacacgg taaagacgaa tatgttctat
      601 taccaatcgc agtttatcgc gttctaaaag atcaaatcga aaaagaatta gcaacctgcg
      661 aagcaaatcg agatgacgag caatcttatg agccatttgt tctcgaagat tatgttgata
      721 atcctgtagc gttagcacga atcaaagccg gtattacaca agaacaatta gctcaacgct
      781 taggtgttag ccaagcatat gttagccaaa ttgagcgtcg caataatgta accaacaaag
      841 tacttgagcg agtgtacaca gccatccaag gaaccacata aatttaggaa atcactgctt
      901 aattcatcat tgccgccgcg aagcggcttg tccttgacag cttgtcactc cccacacaat
      961 gagaattgaa ggattgagga ataagcacaa ccaccccttc catttctaac aaaagaatat
     1021 tcaatgtttc ttgatagtga tgactttaaa tcagtttggg aattagctca taactggatt
     1081 agtgaggata caaccaaaac tgaatctgaa tcaatttctg ctgacctcaa agtggcaatc
     1141 catcagctgt tacatgctat tgtgtcaatc agcattgaaa actgtccacc tgttttagtt
     1201 ggttaattgg tttttacatt attttcgatg cttgaaacgg taagaatcat taccggtttc
     1261 caggatgtcg caatgttgct tttcttgact taccaccttg ctggatatct ataaacgcaa
     1321 ccagtaacca agcagtaaaa aataatgcta tcagctcacg aaccataaaa gagaaaaggc
     1381 tacagcaaca acatgctgct catcagaaac ataaacctgt tgaggagcta aaaaaagcat
     1441 gcattcttta ctggttggaa aatcaaagtt gcagcaatga gcaaactgca aaaaaatttt
     1501 atggatcact ttcttctgag caacagaaac tgctcgttcc aaccaatgca gctaaaacgc
     1561 ttgcaaaagc tatatccgac tatagaaatc gagataaatt agcaaaaaag aataacctac
     1621 cgtattggtt atgaatatta cgacttcggt tgacagttct gataaattaa actgttttca
     1681 aggagtcgtt gacgaatcta caagcacaaa gagatatatc tcgcaaactc aaagtatttg
     1741 aacatgccgc tacatctggg aatattgcat tcacctgcag gcattttggt atatcaagag
     1801 atacatttta tcaatggaag cgggcttatg cagcacgtgg agaaaaaggg ctgatcaata
     1861 gcaaaccatg ccctgaaaac cccaagagac ttttgcaaaa cacctgactt gataaaatcg
     1921 ggcaaagata gtgcgattcc agtctgattt actcaatttt ttatgaattt ctataaaaaa
     1981 tacgtgaatt aatagaaatc tgccttgttt ttttagtatc ttttattaat tttctgtttg
     2041 aaagacgtat ttctcctgcc tcatcaagct ttcagtaaac aatgttggta accttt
//
LOCUS       DAOQLJ010000266         2096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_35443_length_2096_cov_5.285154, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000266 DAOQLJ010000000
VERSION     DAOQLJ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35443_length_2096_cov_5.285154"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="PK522_11865"
     CDS             complement(<1..1321)
                     /locus_tag="PK522_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42472.1"
                     /translation="MAHDSTPNPQLAIILEAIQNRDYPDSIEQIKAYVAMLETEERFY
                     PARQLLSQAREKYRSIRQFQSEPTAVNWWQTDAAQDEVWITQKQAFFTYKNPELPPRT
                     RFIDSLLLLDEIGLRDSTNRDAETLALGGSIYKRKWQHFGQLEDLIEALFFYRAAFER
                     NPQQDKGYGGVNAALILDILADRAKQIARRSGIKLNESEASNFSAQAAAIRQNMVLQI
                     PVWQTELKQLPESDHFWPQVTFAEIYFGLQQYDQTKEWLQKANVAHAINWKKQTIYSQ
                     LLQIGRLQDLTIPKESDQPENWLPAWQILALIIGEDNAQRAFSSGTGKVGLSLSGGGF
                     RASFFHLGVMARLAEIDALRSIEALSTVSGGSIVGAHHYLEVQKLLESKPDKAITRND
                     YIEIVRRIQDNFLKGVQTNIKLQTFASLTDNLDVFFRKKHYSRSHRLG"
     gene            1551..>2096
                     /locus_tag="PK522_11870"
     CDS             1551..>2096
                     /locus_tag="PK522_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42473.1"
                     /translation="MNERNWDRLLKSIEEGLVVPVMGPQLITSLNGSSSFQSQIAQQL
                     LKMYDYALGHDVLMPHQELNDAVTRLKRDHPIQDLYGDIHDVIGQLTPNTDAELPEAI
                     KQITAIRDFRLFVTLTPDDLLARSLQAQVTVNEIIHSPYLPTSEGSDLPNDWRSRPGE
                     VYLLYLFGKSRSAPMFAIHDED"
BASE COUNT          538 a          439 c          479 g          640 t
ORIGIN      
        1 ccccgaggcg gtggctgcga gaataatgtt ttttgcggaa gaatacatcg agattatcag
       61 tcaaacttgc aaatgtttgc agtttgatat tggtttgtac gcctttcagg aaattgtctt
      121 ggatgcgtcg cacgatttcg atgtaatcat tacgtgtaat tgctttgtct ggtttgcttt
      181 caagcaattt ttgtacttcc aagtaatgat gagcaccgac gatgctgccg cccgatactg
      241 tcgataatgc ttcgatactg cgtaatgcat cgatttcagc aagcctagcc atgacgccca
      301 ggtggaaaaa tgaagctcgg aaaccaccgc ccgataacga taatccgact ttaccagttc
      361 ctgatgaaaa ggcacgctga gcgttatctt caccaatgat taacgcgagt atttgccaag
      421 ctggtagcca attttcaggc tgatcgcttt ctttcggaat cgtcaagtct tgtaggcgcc
      481 cgatttgcag caactggctg tatatggttt gttttttcca attgattgca tgagctacat
      541 tggctttttg caaccattcc ttggtttgat catattgctg caagccaaaa taaatttctg
      601 cgaatgtgac ttgtggccag aaatggtctg actctggtaa ttgcttgagt tcagtttgcc
      661 aaaccggaat ctgtagtacc atattttggc gtatcgcagc agcttgtgca gaaaaattgc
      721 tggcttcgct ttcattgagt ttaatgccac tgcggcgtgc aatttgctta gcgcgatcag
      781 ccaagatatc gagaatcagc gcagcattga cgccaccata gcccttatcc tgttgagggt
      841 tacgttcaaa tgcggcgcga taaaagaata atgcttcgat gagatcttct aattgaccaa
      901 aatgctgcca ctttctttta tagatactcc cccctaaggc tagcgtttct gcgtcacgat
      961 tggtcgagtc gcgcaacccg atttcgtcaa gcaataacag tgaatcgata aatcgtgtac
     1021 gaggtggcag ttcaggattt ttatacgtaa agaaagcttg cttttgggta atccaaacct
     1081 catcctgcgc agcatcggtt tgccaccagt ttactgcggt aggttctgat tggaattgtc
     1141 gaatcgagcg atatttctcg cgtgcttggc tgagtaattg tcgagccgga tagaaacgtt
     1201 cttccgtttc tagcatcgca acataggctt taatttgctc gatagagtct ggataatcgc
     1261 gattttggat cgcttctaga atgattgcta attgagggtt tggggttgaa tcgtgggcca
     1321 tgagcaagct ccttgttcga taacatgcgt tcaatcaaaa gcatctgcta ctgttgttct
     1381 ttgataatgg agtacgcaga tgatatgttg tgttcttgtt ctactatgct accgtatttt
     1441 acggtgattt gacgtgaaga aaatcacaag gcctattctg cgctaaaaac taaccacaac
     1501 gacgatttcc gtcataaaat ccggattgtt aaggtaaaga ttgttaaacc atgaatgagc
     1561 gtaattggga tcgtttgctg aaatcgatag aagagggact agttgtgcct gtaatgggtc
     1621 cacaactgat cacttctctt aacggctcat cctcgtttca gtcgcagatt gcccaacagt
     1681 tacttaaaat gtatgactat gcgttaggtc atgatgtgct aatgccacac caagagttga
     1741 acgatgctgt gacgcgcttg aagcgcgatc accctattca ggatctttat ggtgatattc
     1801 atgatgtcat tggacaatta acgcctaata ctgatgcaga gcttcccgaa gcaatcaaac
     1861 aaataactgc aattcgtgat tttcgattgt tcgtaacttt gacacccgat gatttactag
     1921 cacgcagctt acaagcgcaa gtgactgtca atgaaattat tcattcacct tatttaccga
     1981 ccagcgaggg ctctgatttg cctaatgatt ggcgttctcg accgggagaa gtttatctat
     2041 tgtatttgtt tggcaaatca agatctgcgc caatgtttgc gattcatgat gaggat
//
LOCUS       DAOQLJ010000267         2083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_35877_length_2083_cov_4.076430, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000267 DAOQLJ010000000
VERSION     DAOQLJ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35877_length_2083_cov_4.076430"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2003
                     /locus_tag="PK522_11875"
     CDS             <1..2003
                     /locus_tag="PK522_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC42474.1"
                     /translation="SEEYRSKVNAIHQAFNERQEKVISELGEEAEKQEIVLLRTPDGF
                     ALAPSRNHEVIPPEEYDKLPQAEKERILTAITGLQARLEKVLNHLPQWRRERSEHIKQ
                     LNRDIMLSVVAHVIDELRTQYANLPEVLSFLDAVQRDMILHVDDFRKPDESMNISGIP
                     VVAHQTFSNYKVNVLVSNQNSSGAPLVCEDNPTYSNLLGRVEHISQFGALVTNFTLIK
                     PGALHRANGGYLLLDIRKILMQPFAWDALKRALQTREIRIESLGQMYSLISTVSLEPE
                     PIPLAVKVVLFGDRLLYYLLQAYDPEFSELFKVAADFEEVIDRSAEVQQLYAQLIATL
                     VRKESLLPYDRDAVARVIEHSSRLVSDSEKLSMQMRNIADLLREADYWARDAGHATVL
                     ASDVQHAIDAQIHRQDRIRDRLYEAILRGTLMIDTAGAVIGQVNGLAVIELGSFAFAQ
                     PTRITATTRLGKGDFINIEREAELSGAIHSKGVLILAAFLASRYAKNQPLALSASLAF
                     EQSYGKIDGDSASLAELCALLSNLAHVPILQTLAITGSVNQLGQAQAIGGVNEKIEGF
                     FDICVARGLSGQQGVVIPVSNVKHLMLRRDVIDAIAKGLFAIYAVENVDQAISLLTGI
                     SAGEVDEKGIYPKGTINDLVIERLAELTKITQSLSSIEKGEK"
BASE COUNT          578 a          427 c          499 g          579 t
ORIGIN      
        1 aaagcgaaga atatcgcagt aaagtcaatg caatccatca agcgtttaat gaacgccagg
       61 aaaaagtgat tagcgaactg ggggaagaag cggaaaaaca agagatcgta ttgctgcgta
      121 cgcccgatgg gtttgctttg gcaccgagcc gcaatcatga agtgattcca cccgaggaat
      181 acgacaaact ccctcaagca gaaaaagaac gtattttaac ggcgattact ggactacaag
      241 cgcgcttgga gaaagtgctt aatcatcttc cgcagtggag aagggagcgc agcgagcata
      301 ttaagcagtt gaaccgagat attatgcttt cagtcgttgc ccatgtcatt gatgaactac
      361 ggacacaata tgctaatttg cccgaagtgc tgagctttct cgacgcagtg cagcgcgaca
      421 tgattttgca tgtcgatgat tttcgtaaac ccgatgaatc gatgaatatt tcaggtattc
      481 cagttgttgc ccaccaaacc ttttccaatt ataaagtcaa tgtgttggtc tcgaatcaaa
      541 attcctccgg tgcgccttta gtctgcgagg ataatccaac ttatagtaat ttgcttggca
      601 gggtagagca tatttcacaa tttggcgcat tggtaacaaa ctttaccttg attaaaccag
      661 gtgcattgca tcgagccaac ggtggttatt tactgctgga tatccgcaaa atactcatgc
      721 agccgtttgc ctgggatgca ttaaaacgcg ctttacagac acgcgagatc cgcattgagt
      781 cattaggcca gatgtatagc ttgattagta ccgtttcgct ggaacctgag cctattccgc
      841 tagcggtaaa agtggtttta tttggcgacc ggctacttta ttacctgttg caggcttatg
      901 atcctgaatt tagtgaattg tttaaggttg ctgcagactt tgaagaagtt attgatcgaa
      961 gtgcagaagt acagcagttg tatgcacagc tcattgcaac gttagtgcgt aaggaatcct
     1021 tattgcctta tgatcgtgat gccgttgcac gggtcatcga gcatagctcg cgtttagtca
     1081 gtgactcaga aaagctttcc atgcagatgc gtaacatcgc tgacttgtta cgagaagccg
     1141 attattgggc acgagatgcg ggtcacgcaa ccgtgctggc gagcgatgta caacatgcca
     1201 tcgatgcaca aattcatcgc caagatcgta ttcgggatcg cttatatgaa gcaattctac
     1261 gcggcacttt gatgattgat actgccggtg ctgttattgg acaggtcaat ggattggcgg
     1321 tcatcgaact cggtagcttt gcttttgctc aaccaacacg catcacagca acgactcgac
     1381 tcggtaaagg ggattttatc aatatcgagc gagaggcgga gctttcaggc gcaattcact
     1441 caaaaggcgt gttgattctg gctgcattct tggcctcgcg ctacgctaaa aatcaaccac
     1501 ttgcactatc agcgagtctg gcatttgaac aatcctacgg aaaaatcgat ggtgatagtg
     1561 cttcactcgc agaattgtgt gcattgcttt ctaatttagc gcatgtacct attcttcaaa
     1621 cgctggcgat caccggctct gtcaatcaac tcgggcaagc gcaagcaatt ggcggtgtga
     1681 atgaaaagat tgaaggtttt tttgatattt gtgtcgcacg gggcttatcg ggtcaacaag
     1741 gagtagtgat tcctgtttca aatgtaaagc atttaatgtt acggcgggat gtgattgatg
     1801 ccatcgcaaa agggcttttt gcaatttatg ccgtggaaaa tgtcgatcaa gcgatttcat
     1861 tattgactgg tatttccgca ggtgaagttg atgagaaggg gatttatccc aaaggaacga
     1921 tcaatgattt agtcatcgaa cgcttggctg agctgactaa aatcactcaa tcattatcgt
     1981 caatagaaaa gggtgagaaa taatcagtta atttttctcg atcaagctta aaaattaagc
     2041 ttatttgtgt atagaattcc ttaacgcttt aaccattaaa ata
//
LOCUS       DAOQLJ010000268         2076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_36098_length_2076_cov_6.633350, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000268 DAOQLJ010000000
VERSION     DAOQLJ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36098_length_2076_cov_6.633350"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PK522_11880"
     CDS             complement(<1..540)
                     /locus_tag="PK522_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNC42475.1"
                     /translation="MAINEKATTFTYGTRKNDTIDAQYLLGDMIDGLQGDDTITGLSG
                     NDTLLGGSGRDTIFGLDGNDLLDGGAGNDKLYGGAGDDLIRPDTGAIGSDLIDGGDGI
                     DTLDYSVSAAVSGVTVDLRKIVLQDTKGAGKEIILNIENINGTNFVDTLTGNDTRNVL
                     VGGNGGDTIYALDGHDQIDG"
     gene            complement(795..959)
                     /locus_tag="PK522_11885"
     CDS             complement(795..959)
                     /locus_tag="PK522_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42476.1"
                     /translation="MMTKKRITTYVLALMVAMVFSSAFANPPQTPEDCQKIYAGDDVK
                     VRTCIDSLKK"
     gene            complement(1353..1943)
                     /locus_tag="PK522_11890"
     CDS             complement(1353..1943)
                     /locus_tag="PK522_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC42477.1"
                     /translation="MTIQPKDVVALTAANPGVLKSMLDCVLRFNQPHSGRIMLDGIDI
                     ATLKSEEIGTKVAWISPDMPLLEDTLAANIAYGTMHCATEAQLTAIARVSHVADFARE
                     MPYGLQTRINKDGLTSSEALKQCVLLARALLKNPAIVIIDESFVEFELSNEKVDHALD
                     VLMQGRTSIIISTRQPMIQKAQKMVCIDDLMQMTSK"
BASE COUNT          610 a          489 c          382 g          595 t
ORIGIN      
        1 accatcaatc tgatcgtgcc catctaacgc ataaatcgtg tcacccccat ttccacctac
       61 cagaacgttt ctagtatcgt taccagtcag cgtatctaca aaattggtgc cattgatatt
      121 ctcgatatta agaataatct ctttccctgc acccttggta tcttgtaaga ctatcttacg
      181 caaatcaacc gttacaccgc tcaccgcagc tgaaacacta taatccagag tatcaatgcc
      241 atctcctccg tcgatcaaat cgctcccaat tgcacccgta tcgggtctga tcaagtcatc
      301 accagccccg ccatacaatt tgtcattacc ggcgcctcca tcgagtaaat cattcccatc
      361 taaaccaaaa atcgtatcgc gaccgctacc gcccaataat gtatcattcc cacttaggcc
      421 tgtaatcgtg tcatcaccct gcaagccatc gatcatgtca ccaagcaaat attgcgcatc
      481 aatcgtgtcg tttttgcgcg taccataggt aaaagttgtt gccttctcat taattgccat
      541 ttgcattctc ctttatcatc agaattatag ttattgttga tacttacatt tttgattgta
      601 atcctttaat actcttcgat attgatccaa atcaatatcc aagcatcctc cactaatagt
      661 tggacaagca cgaacatagg gacaagaaag caagggcaat ataataatta ccccttcaaa
      721 ctaagtggaa cacctaattt gaagaggttt cgccctgata atattagggc aggaggaaaa
      781 taaatggagc gtacttattt cttaagacta tcaatacagg ttcggacttt tacatcatcg
      841 cctgcataga ttttttggca atcctcaggc gtttgaggcg ggtttgcgaa ggcagacgag
      901 aagaccatcg caaccatcaa ggccaaaaca taagtggtaa ttcgtttttt tgtcatcatg
      961 attaaactcc tatttaacaa tgagtaaaga aaactgacga atacgccagt aattcttatt
     1021 tagcaatgag tgtgccaacc tgacaaaaat gctttagaac aacaggttat cgaaattgct
     1081 catcgataat ccgatcaatc gctgcacatt ttcacgtaat gatgtcgttt tgaaccgact
     1141 tatagatcga cagaataagc tatcgcaacg attagacttg aaatagcgct gtctttaatt
     1201 tgaaacaaat acccgtaggc gatcgaatcg taaaatttgc taccaatcac tgcgatgtta
     1261 tgtgacttat ttaagaagct gaggaaattc ggctatttac aaatatttac gaaaataaga
     1321 tgtcttacac gcgatgttct ccgtgttcac tatcatttag aggtcatttg catcaaatcg
     1381 tcaatacaaa ccattttctg agctttctgg atcataggct gacgggttga aataatgatc
     1441 gaggttcgcc cttgcatcaa aacatcgagg gcatgatcaa ctttttcgtt actgagttca
     1501 aattcaacga aggactcatc aataatgaca atcgctggat ttttaagtaa tgcacgcgcc
     1561 aataacacgc attgcttgag tgcctcagag gaagtcaatc catctttatt gatgcgagtc
     1621 tgcaagccat agggcatttc tcgtgcaaaa tcagcaacgt gacttacccg ggcgatagcg
     1681 gttaattgcg cttctgttgc acaatgcatc gtcccatatg caatattggc tgccaacgta
     1741 tcttcaagca acggcatatc gggactaatc catgcaacct ttgtacctat ctcctcgctc
     1801 ttcagcgtag caatatcgat tccatccagc attatccttc ctgagtgcgg ttgattaaat
     1861 cgtagtacac aatccagcat tgatttgagc accccagggt ttgctgcggt taatgccacc
     1921 acgtctttgg gttgaattgt caaaaaacta tgggacgatg aatcattaag cgaatcacga
     1981 ccttgcggtt taagattctc aataacgagc gcgccttgaa tcgttccaag ggcgattcaa
     2041 ggcgcgctcg ttattgagaa tcttaaaccg caaggt
//
LOCUS       DAOQLJ010000269         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_36945_length_2050_cov_5.945363, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000269 DAOQLJ010000000
VERSION     DAOQLJ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36945_length_2050_cov_5.945363"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PK522_11895"
     CDS             <1..241
                     /locus_tag="PK522_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNC42478.1"
                     /translation="GATVCYLDQTGVERTVSIVGIDEIDPFKGYISWISPVARALISA
                     QEGDTVYLRTPTSVEKLEILSVIYQSIAIPAFQPI"
     gene            496..1320
                     /locus_tag="PK522_11900"
     CDS             496..1320
                     /locus_tag="PK522_11900"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC42479.1"
                     /translation="MESEYANRTNRENQLIGDQQGFFTPGFCYVCSKNVDFFSNFAHA
                     FLNADARKIPNWREHMVCPHCHLNNRMRGAIQVFEQLCEAKLDDNIYLTEQKTPLYQW
                     FQQNYLKVIGSEYLANTVPYGSLDKDRIRNETLTGLTFETNTFDYILSFDVFEHIPEY
                     QVAFQETLRCLKPRGKLIFTVPFNRGSDNSIVRARLASDGTIEHILPPEYHGDPLNSK
                     GVLCFYHFGWELLDQLCEIGFSSATAYLYWSEELGYLGFEQILFVAQKSDAEKMLS"
     gene            complement(1835..>2050)
                     /locus_tag="PK522_11905"
     CDS             complement(1835..>2050)
                     /locus_tag="PK522_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42480.1"
                     /translation="EFWFSTHSPVPRKPAPSFIHLVSLACNFSLSEIGFTDTSIELAM
                     GDTRIKKPTAMKDSQFFICQALAGESL"
BASE COUNT          608 a          379 c          419 g          644 t
ORIGIN      
        1 tggcgcaacc gtttgctact tagatcaaac gggtgtggag agaacggtat ctatcgtcgg
       61 tatcgatgaa atcgatccct ttaaaggtta tattagctgg atctctccgg ttgcacgggc
      121 gctgatctct gcacaagaag gggataccgt ttatttgcgc acaccaacga gtgtcgaaaa
      181 gctggaaatc ttgtcggtta tttaccaatc gattgcaata ccggcgttcc aaccgattta
      241 agttaaattt ctttttgatt caagtgccta attggtaaat aaactcgttt ttgtgcaatt
      301 taaaaaatga tgcacctgct cattaaaaag atccgtaatc gtgctcaatt tttttggaat
      361 cgaatatttt gtactaaaac tagtaaaaat gatttgttga cgcagaaaca ttgtcgacag
      421 cgcagccaat ttgatcgtcc attaaattta gagtctattt cctcattcgc agcatatgtg
      481 acatatcacg aaaagatgga atcagaatat gccaatcgaa caaatagaga aaatcaatta
      541 ataggcgatc agcaagggtt ttttactcct ggtttttgct acgtttgtag caagaatgtg
      601 gattttttca gcaactttgc gcatgctttc ttgaatgctg atgctcgtaa aattccaaat
      661 tggcgggaac acatggtatg tccacattgc catttgaaca accggatgcg cggagcgatt
      721 caagtctttg aacaactatg tgaagccaaa ctcgatgata atatctattt gaccgagcaa
      781 aaaactcccc tctatcaatg gttccagcaa aattatttaa aggttattgg tagtgagtat
      841 ctagccaata cggttcctta tggaagcctt gataaagatc gtattcgaaa tgagacgctt
      901 acaggtttga cctttgaaac taacacgttt gactatattt tatcctttga tgtattcgag
      961 catattcctg aataccaggt tgcttttcaa gaaaccttac gttgcttaaa acccagagga
     1021 aagctaatct ttaccgtacc attcaaccgt ggttcggata acagcatagt acgagcacgg
     1081 ttagcttcag atggaacaat agaacatatc ttgccaccgg aataccatgg cgatccactc
     1141 aatagcaaag gtgttttgtg tttttatcat tttggatggg aactactcga tcaactatgt
     1201 gaaataggct tttcatctgc aacagcctac ttatattggt cggaggaact aggttacctg
     1261 gggtttgagc aaatactttt tgttgctcag aaaagtgatg ctgagaaaat gctgagttag
     1321 tgattttttt cgcgttttga ttatactgtc attgattcgc tcatcgcatc acctccttaa
     1381 tagtcgaaac agtactcacc agcagtttct ttctgctcag tgattttatc gatttgtgac
     1441 cagtattcaa tctgtttgtt tcggtactga acgatgttga acttgtgcat aatgcctagc
     1501 tttggcgact gggttctcag acaactttat atttacaagc ataatcaccc ctgagcaaaa
     1561 ttccattcta gtttgattat tcaaggaaag acaaggttgc tgataggttg tatatgaatt
     1621 ttattggtga agattagcgc tattgctgag gtatcctaag ttgaagctta ttgaacacca
     1681 tgtgaatcat actcatcagg ctaaaaatat tacgaatcca tagaatttga tcttttatat
     1741 agaatcaggg ctgtttccac gatcttagta aatttgataa agcatccaga tagaacatta
     1801 acagggagat aatcgatctt atggaaaagg ttaatcataa tgattcaccg gctagcgctt
     1861 ggcagatgaa aaattggcta tccttcattg cagtaggttt ttttattctg gtatcaccca
     1921 ttgctaattc gatggatgta tcggtaaaac cgatctctga caaactaaaa ttgcaagcaa
     1981 gtgaaacgag atggataaaa ctgggtgccg gttttcgtgg aactgggcta tgggttgaaa
     2041 accaaaactc
//
LOCUS       DAOQLJ010000270         2010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_38388_length_2010_cov_6.213811, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000270 DAOQLJ010000000
VERSION     DAOQLJ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38388_length_2010_cov_6.213811"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /gene="metK"
                     /locus_tag="PK522_11910"
     CDS             complement(<1..1023)
                     /gene="metK"
                     /locus_tag="PK522_11910"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNC42481.1"
                     /translation="MSNYLFTSESVSEGHPDKVADQISDAVLDAILQQDPNARVACET
                     MCSTGLIVLSGEITTHASVDYNIIARETVKRIGYTSSDIGFDSTTCAVLTAFNKQSPD
                     IAQGVNRSKEEEMDQGAGDQGLMFGYACDETSQLMPMPIYYAHRLVERQALLRKNGRL
                     PWLRPDAKSQVSARYIDGKPHEVETIVISTQHDPDIPYAALKEAVIEEIIKPVIPETI
                     RSKQIQYLVNPTGRFVVGGPMGDCGLTGRKIIVDSYGGSAHHGGGAFSGKDPSKVDRS
                     AAYAGRYVAKNIVAAGLASRCEVQIAYAIGVAKPVSLMVDTFGTGKISDEALVQLIEQ
                     HFDLRPR"
     gene            complement(1255..>2010)
                     /locus_tag="PK522_11915"
     CDS             complement(1255..>2010)
                     /locus_tag="PK522_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNC42482.1"
                     /translation="LTEPGKELVKIVERILLDTKNIKRLAEQFSQHDQGQLTVATTHT
                     QAQYALPPVIREFKQLFPKVHLILHQSSPDEIVSMLLDGQADIGIATEAVKNTNELVS
                     FPFYTWHHVIIVPTGHPLQSIQPLTLEAIAEFPIITYHQGFTGRSRIDRAFEQENLTP
                     EIAMSALDADVIKTYVTLGLGVGIIASVAFMPERDFQLVKLEAPHLFEKNTTYIAIRE
                     NHYLRGYAYRFIELCVPTLSESDIRANTKPEID"
BASE COUNT          575 a          429 c          437 g          569 t
ORIGIN      
        1 tctcggacgt aaatcaaaat gttgttcgat caattgcact aacgcttcat ccgatatttt
       61 accggtacca aaagtatcga ccataagcga aaccggtttt gcaacgccaa tcgcgtaggc
      121 gatttgaact tcacatcgac ttgctaaacc agcagcaacg atattttttg ccacatatct
      181 tccagcgtaa gctgcagaac gatcgacttt ggaaggatct ttgccggaaa atgcaccacc
      241 accatgatga gcagagccac catagctatc gacgataatt ttacgtcctg ttaatccaca
      301 atctcccatt gggccaccga ctacaaaacg accggtagga ttaactaagt attgaatctg
      361 tttgcttctg atggtttcag ggataacggg tttgatgatt tcttcaatga ccgcttcttt
      421 aagcgctgca taaggaatat ctggatcatg ctgagttgaa attacgatag tctcaacttc
      481 atgcggcttg ccatcaatat accgagctga tacttgagat ttagcatcag gccttaacca
      541 gggtaaccgc ccattttttc tcaataacgc ttgtcgttcg accaaacgat gcgcgtagta
      601 gattggcatg ggcattaact gagaagtttc atcgcaagca taaccaaaca tcaaaccttg
      661 gtcgcctgca ccttgatcca tttcttcctc tttgctgcga ttgacacctt gtgcgatatc
      721 aggggattgt ttattaaacg cggtaagaac agcacaagtc gttgaatcaa aaccaatatc
      781 agaacttgta taaccaattc tcttaaccgt ttcacgagca atgatattgt aatcgactga
      841 agcatgcgta gtaatttcac cggataaaac aatcaaaccg gtgctacaca tagtctcgca
      901 agcgacccgt gcattggggt cttgttgcag tatcgcatcg aggacggcgt ctgaaatttg
      961 atcagcgact ttatcaggat gcccctctga aacggactcg gaagtaaaga gatagttgct
     1021 catttcgtaa gtatacccac ctaaaatatt caattataac aaaaattctt acattaactt
     1081 tctagataac taggttgcca atacaaacag tttcatagcg tacgactaaa aatagtaatt
     1141 gcttagaata aataagcagg gtaaaaatgc atatatgtat caatagcact tgttggattt
     1201 ctaaatcagg tgatagcgcc tatttttttg ttaggcataa cctctctctg acgtttaatc
     1261 aatttcaggt tttgtgttag ctcggatatc tgattcactc agggtaggca cgcatagttc
     1321 gatgaatcga taggcgtagc cgcgcaagta atgattctca cgaatcgcaa tatacgttgt
     1381 gtttttctca aagaggtgag gtgcttcgag tttgactaat tgaaaatcgc gctctggcat
     1441 aaaagcgact gaagcaataa tcccaacccc aagtcctagc gtgacgtaag ttttgattac
     1501 atccgcgtcg agtgccgaca tggcaatttc aggtgtcagg ttttcttgct caaaagctcg
     1561 atcgatacgg gaacggccag taaatccctg atgataggtg atgatcggaa attcagcgat
     1621 agcctctaat gtcaagggtt gaatcgactg tagtggatgg ccagtcggta caatgatcac
     1681 atggtgccaa gtgtaaaacg ggaatgaaac taattcgttg gtatttttca cggcttccgt
     1741 ggcgattcca atatcggcct gcccatcgag taacatggaa acgatttcat ctgggctaga
     1801 ttggtgcaaa atcaaatgaa ccttaggaaa tagttgctta aattcacgga tgacgggcgg
     1861 caaagcatat tgggcttgag tgtgggtggt ggcaacggtt aattggccct gatcatgctg
     1921 actaaactgt tcagccaggc gtttgatatt tttagtatcg agtagaattc gttccacgat
     1981 tttcacaagc tctttacccg gttcggtgag
//
LOCUS       DAOQLJ010000271         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_39014_length_1993_cov_4.475748, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000271 DAOQLJ010000000
VERSION     DAOQLJ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39014_length_1993_cov_4.475748"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="PK522_11920"
     CDS             <1..1026
                     /locus_tag="PK522_11920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNC42483.1"
                     /translation="IIYQDERMRELWGGKASKISPYDAFYAAIHPKDRISYQIALEKA
                     TNPSGDGEFNIDVRVIHSTNGLERWIHSIGKVHFYEGVATRLIGIVQDITQQKLLEKE
                     LQSYRAEVESLFKQQVAIQTASAIAHELNQPLTVISAYSELALITLENNPANLDKIKQ
                     SLHNCVIQSQRAGDTLRELIASLQKTDAHIERLDLNQVILDALRTVRSDGFSDFQQIL
                     QFELNLPAVLANRVQIKKVVVNLLRNAVEAMNSTNVATSEITISVKTMAKEKYALVTI
                     EDKGPGIDPKIRHQIFDPFFTTKPTGIGMGLSISRALIEANGGQLWVDPHTPTGARLN
                     FTLPFAA"
     gene            1023..1649
                     /locus_tag="PK522_11925"
     CDS             1023..1649
                     /locus_tag="PK522_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC42484.1"
                     /translation="MIAHPPTVFIIDDDATVREAITLLLEHEEIHVQSFASADAFLSA
                     YHPDFIGCILLDICMPGKDGMQLHEELKQRQSKLPIIFLTGYGDIPQSVKAIKAGAID
                     FLTKPVTRVKLLASIRSAFLEAYKTQHVAEHHQAILSRIATLTEREQEIMTLVVQGYS
                     NKEIAQHLHISFRTVEIHRSHILHKTGAHNLLDLVRIAQEGGLSPSEI"
     gene            1788..>1993
                     /locus_tag="PK522_11930"
     CDS             1788..>1993
                     /locus_tag="PK522_11930"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNC42485.1"
                     /translation="MPRSFHIIKYLILAAISSLFFATFIHAEQPKVPFTVEVLTSGLD
                     HPWSMAFLPDGRIMITERAGRLRIV"
BASE COUNT          580 a          476 c          420 g          517 t
ORIGIN      
        1 attatttatc aagatgagcg tatgcgcgaa ctctgggggg gaaaagctag caaaatctct
       61 ccatacgacg cattttatgc ggctattcat ccgaaagacc gtatctctta tcaaatcgct
      121 ttagaaaaag caacgaatcc gagtggtgat ggtgaattca atatcgacgt acgagtcatc
      181 cattcgacaa acggtttaga acgatggatt cactccattg ggaaagtaca tttctatgaa
      241 ggggtagcga ctcgtctcat tggcatcgta caagatatca cgcagcaaaa attgctagaa
      301 aaagagctgc aaagctatcg tgctgaagta gaatcactat ttaaacagca agtcgcaatc
      361 caaaccgctt ctgcgattgc ccatgagctt aaccaaccgc ttacggtaat ctctgcttat
      421 agtgaattag ccctgattac attagaaaat aacccggcta atctggacaa aatcaagcaa
      481 tcactccaca attgcgtcat tcaaagtcag cgggcgggtg atacactgcg tgaattgatt
      541 gcatctttac aaaaaaccga tgctcatatt gaacgactgg atctcaatca agttattctc
      601 gatgcgctgc gcacggtgcg cagcgatggg tttagtgatt ttcagcaaat cctgcagttc
      661 gaactcaatc ttcctgccgt tttagccaat cgtgtccaaa tcaagaaagt cgtggttaac
      721 ttattaagga acgcagtaga agcaatgaat tctacaaatg ttgccacttc agaaattacc
      781 atatcagtca aaacaatggc caaagagaag tatgcattag taaccattga ggataaaggt
      841 cccgggatcg atcccaaaat taggcatcaa atctttgatc cattctttac cactaaacca
      901 actggtatcg gtatgggttt gtctattagc cgcgcgctga tcgaagccaa tggcggtcaa
      961 ctctgggttg atccccatac acccacgggt gcaaggctta actttaccct tccttttgcc
     1021 gcgtgattgc gcatccaccc accgttttta ttattgatga tgatgccacg gtgcgcgaag
     1081 cgattacgct attactcgaa cacgaagaaa tccatgtaca atcctttgcg agcgctgatg
     1141 catttttaag tgcctaccac cctgatttta tcggctgcat tttattagat atctgtatgc
     1201 ctggaaaaga tggcatgcag ctccatgaag aactcaaaca gcggcagagt aagttgccta
     1261 tcattttcct gacgggatac ggtgatatac cccaaagcgt caaggccatc aaagcaggtg
     1321 ctatcgactt tctaacgaaa ccggttaccc gagttaagct gcttgcgagc attcggtcag
     1381 cttttctgga ggcatacaaa acccagcatg tcgctgagca tcaccaagca atcctttcgc
     1441 gcatcgcaac cctaaccgag cgtgagcagg aaatcatgac tctcgttgtc caaggttatt
     1501 ccaataaaga aattgctcaa cacctacata tcagtttcag gaccgtagaa atccaccgct
     1561 cgcacattct gcacaaaacg ggcgcccaca atctgctgga cctagtgcgt attgcacaag
     1621 aaggcggact tagtccttct gaaatctagt catcaccgca aactagcaag cgctagggat
     1681 atgcttcgga atccttcttg caaaaaccca tggactaaaa agagatctat taatacactt
     1741 aaaacttcca gattccagat ggttgctaac gaaaggggaa aaacagcatg ccgagatcgt
     1801 ttcatatcat taaatacctc attctagctg ccatcagcag tttgttcttt gcaacattta
     1861 ttcatgcgga acagcccaag gttccattta ctgttgaagt gttaaccagt gggctcgacc
     1921 acccctggag tatggctttt ttaccggatg gacgtattat gatcactgaa cgcgccggtc
     1981 ggttgcgtat cgt
//
LOCUS       DAOQLJ010000272         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_39286_length_1985_cov_5.562694, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000272 DAOQLJ010000000
VERSION     DAOQLJ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39286_length_1985_cov_5.562694"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1156)
                     /locus_tag="PK522_11935"
     CDS             complement(83..1156)
                     /locus_tag="PK522_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HNC42486.1"
                     /translation="MSIEIRNLHKQFGSFTALNNVSLDIQPGELLALLGPSGSGKTTL
                     LRVIAGLETVDSGQVLFNNEDATHQHIHERQVGFVFQHYALFRNMTIFENVAFGLRVR
                     PKSVRPDNEEINKRVTDLLKLVQLDWLGDRYPHQLSGGQRQRIALARALAVEPKVLLL
                     DEPFGALDAKVRKELRTWLRRLHDDMHITSVFVTHDQEEALEVSDRVVVMNEGRIEQI
                     ATPDEVYEKPANPFVYEFLGHVNLFHGRVHKGRAWIGDIEIDAPDHAEAEDLAAVAYV
                     RPHDIEVDREPNGESALAAQIVHILSVGPIVRLELAREENQGKNLIQVEISKERFRGL
                     QLEKGDRVFIKPRRLDLFPHLTH"
     gene            complement(1146..>1985)
                     /gene="cysW"
                     /locus_tag="PK522_11940"
     CDS             complement(1146..>1985)
                     /gene="cysW"
                     /locus_tag="PK522_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HNC42487.1"
                     /translation="RTRITQEPRWVRYTLITIALTFLALFLFVPLISVFYEALKKGVA
                     VYIAAISDPDALAAIKLTLTATAIAVPLNLIFGVAAAWAIAKFEFKGKNVLITLIDLP
                     FSVSPVIAGLIFVLVFGMQGWLGPWLAEHDIRIIFAVPGIVLATLFITVPFIARELIP
                     LMQAQGTEEELAAVTLGANGWQLFWRITLPNIKWGLLYGVILCNARAMGEFGAVSVVS
                     GHIRGSTNTLPLHVEILYNEYNFAAAFAVASLLALLALITLVVKTFIESKVSQQKGTH
                     HEH"
BASE COUNT          549 a          462 c          461 g          513 t
ORIGIN      
        1 ctttcttgta tacaaaacct ccgccacggt taagttaatt ttgcaggacc gactatttag
       61 ccaaacgact tttacaaaga actcagtgtg tcaaatgggg aaacaaatcc aatcggcgcg
      121 gtttgatgaa aactcgatca cctttttcca attgtaatcc acgaaagcgt tctttgctga
      181 tttctacctg aatcaaattc ttaccttgat tttcctcgcg cgcgagttcg agtcggacaa
      241 taggcccaac cgatagaata tgcacgatct gtgcagccag cgctgattcc ccattgggtt
      301 ctcggtctac ttcgatatcg tgtgggcgca cataagccac ggctgctaaa tcttctgcct
      361 cagcatgatc gggtgcgtcg atctcgatat cgccaatcca agcacgacct ttgtgaacgc
      421 ggccatgaaa caaattgaca tgccctaaaa attcataaac gaatggattt gctggctttt
      481 catagacttc gtcgggtgtt gcaatttgtt caatccgccc ctcgttcatg accacaaccc
      541 gatcggatac ttcgagtgct tcttcctggt cgtgggtcac gaaaacactg gtgatatgca
      601 tatcatcgtg caatcttctt agccaggttc gtaattcttt acgtactttt gcatcgaggg
      661 caccaaacgg ttcatcgagt aataatactt tgggttcaac ggcgagcgcg cgtgcgagtg
      721 caatgcgttg gcgctgccct cctgagagtt ggtgcggata acgatctcct agccaatcaa
      781 gctggactaa tttgagcaga tccgtcactc gtttattaat ttcttcgtta tcgggacgaa
      841 ccgattttgg gcgcacgcgc aggccaaatg cgacattctc aaagatagtc atattgcgaa
      901 ataatgcgta atgctgaaaa acaaatccaa cctgcctttc atgaatgtgt tgatgcgtcg
      961 catcttcatt gttgaatagt acttgcccgg aatcaaccgt ttctagacca gcgattactc
     1021 tcagtaaggt ggttttaccc gatcctgatg ggcctagcaa agccaataat tcacccggtt
     1081 gaatatccaa gctaacgtta tttagcgcag taaatgagcc aaattgttta tggagattac
     1141 ggatttcaat gctcatgatg agtgcctttt tgttgggaaa cttttgattc aatgaatgtt
     1201 ttgacaacca gcgtgatcag cgccaacagt gctaacagcg aagcgactgc aaacgccgca
     1261 gcaaaattgt attcgttata taaaatttcg acgtgcagcg gtaaggtatt ggtactgcca
     1321 cgaatatgcc cggataccac agaaactgca ccaaattcac ccattgcacg ggcattacag
     1381 agaatgacgc catataacag accccattta atattgggta gcgtaatacg ccaaaataac
     1441 tgccaaccat tggcgcctag ggtcacagcg gcgagttctt cctcggtccc ttgggcttgc
     1501 atgaggggaa tgagttcgcg tgcaatgaag ggaacggtaa tgaagagcgt agcgagtaca
     1561 atgccgggaa ctgcgaagat gattctgata tcgtgttcgg ctaaccaagg gcctaaccac
     1621 ccttgcattc caaaaacgag cacaaagatt aggccagcaa taaccggtga aactgagaat
     1681 ggtaaatcga tcaaggtgat cagaacgttc ttacctttga attcaaattt agcaatggcc
     1741 caggcggcag caacgccaaa aatgagatta agtggcactg caatggcggt tgcagttaac
     1801 gtaagtttga tcgcagcgag tgcatcggga tcgctgatcg ctgcaatata aaccgcaacc
     1861 ccctttttaa gggcttcata aaaaacggaa atgagcggca caaatagaaa tagtgcgaga
     1921 aatgtgagcg caatcgtaat cagtgtgtag cgaacccatc gcggttcttg agtaattcga
     1981 gtgcg
//
LOCUS       DAOQLJ010000273         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_39661_length_1976_cov_5.395627, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000273 DAOQLJ010000000
VERSION     DAOQLJ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39661_length_1976_cov_5.395627"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PK522_11945"
     CDS             complement(<1..745)
                     /locus_tag="PK522_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNC42488.1"
                     /translation="MIRELQQFKIALDAHAIVAITDYKGKICYVNNKFCEISKYSAEE
                     LVGKDHRIINSGYHSKEFFRNLWDTIKSGKIWKGEIRNRAKDNSIYWVDTTIVPFLDD
                     EGKPYQFIAIRAEVTLRKQLEDEILAKVAWQTAILSYAGHAMIATNPEGVIQTFNPTA
                     EDMLGYKAEELVGKATPAVFHEPNEVIDRAAKLSTELGIAIEPGFEVFVARARKNHAY
                     EDEWTYIHKDGTHFPVRLIVTAIKNFNEII"
     gene            1076..>1976
                     /locus_tag="PK522_11950"
     CDS             1076..>1976
                     /locus_tag="PK522_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNC42489.1"
                     /translation="MAESFDVVIVGGGPVGMALALRLREWNIATLLLEAQPLLTNPVT
                     DSRPLALSYGSQLILKRLDVWDNLVSVTPIQTIHISNHGHAGQTLIQSEDAGVPVLGS
                     VVNYHGLYDALYQMMRQQNVTFQLGAKVTSLIHQPDHVQVDYEQSGETHRVDAKLLVL
                     ADGGKLASQVEGIHFHTHDYQQWAVVANIQTSIDQRGIAYEHFTLDGPVALLPSGTGY
                     ALVWTVPAEQVDRILNWDDATFLSELQVHFGDMAGKFVSCGKRTGFPLALKYASPTIA
                     HRTVLIGNASQTLHPVAGQGFNLG"
BASE COUNT          516 a          379 c          444 g          637 t
ORIGIN      
        1 caattatttc attaaaattt tttatagcgg ttactatcaa acgaacaggg aagtgggttc
       61 catctttgtg gatataggtc cattcatctt cataagcatg attttttcgt gcacgagcaa
      121 caaacacttc aaatccaggt tcaattgcaa ttcccagctc tgtagataat ttagctgcac
      181 gatcaattac ttcattcggt tcatgaaaaa cggctggggt tgctttgcca accaattcct
      241 ctgctttata accaagcata tcttcagccg tgggattgaa tgtttggata acgccttctg
      301 gattagtagc tatcatggca tggccagcgt aactcaatat tgcagtctgc caagctactt
      361 ttgctaaaat ttcatcctct aactgtttgc gcagtgttac ttcagcacgt atagcgatga
      421 actggtaagg tttgccttca tcgtcgagaa aaggcacgat ggttgtatca acccaataga
      481 tcgaattgtc tttagcacgg ttcctaattt cacctttcca gatttttcca cttttgattg
      541 tgtcccataa atttctgaaa aattctttcg agtggtaacc tgaattaata attctgtgat
      601 ctttcccgac taattcttca gcagaatatt ttgagatttc acaaaatttg ttattcacat
      661 aacaaatttt gcctttgtaa tcagtaattg caacgatcgc atgcgcatca agtgctattt
      721 tgaattgctg gagttctcta atcatgattc tatgtgtaaa aacttatgag cctacactgg
      781 aatataatta aaactagaat agcaactgtc aaattaatat tttgtgatgt ggtttttgtt
      841 ttctaacgcg ttgagaatga tttcagattt atcaggaata tttgaatttt tcggtaaatc
      901 tctgcgatga atttatcaga gcacagattt gattaggcag gcgttttgtc taaagtatga
      961 gttgtgatat tctgcttggc cttagtgaga agtatgaaat ttactccgga aagaaaattt
     1021 ttgactagac tgatttgagt gatgatcaat tctataaata ataggcttag ctatcgtggc
     1081 tgaatcattc gatgtagtta ttgtcggcgg tgggccagtt gggatggcgc tggcattgcg
     1141 cttgagagag tggaatatcg cgacgctgtt gctggaagcg caacccttat taaccaatcc
     1201 tgttaccgat tctcgaccct tggcgttgtc gtatgggagc cagctcattt taaagcggtt
     1261 agatgtatgg gataacctag tgtcggttac cccgattcag acgattcata tttcgaatca
     1321 cggtcatgcg ggacaaacgt tgatacaatc tgaagatgcc ggggttccag tgctcggtag
     1381 tgtcgttaat taccatgggc tgtatgatgc cctttatcag atgatgcgtc agcagaatgt
     1441 tacgtttcaa ttgggcgcca aagtgacttc attgatccat caaccggatc atgtgcaggt
     1501 tgattatgaa caatcgggag aaacgcatcg cgtggatgct aaattgctgg tgctcgccga
     1561 tggcggtaaa ctggcttcac aggtggaagg aattcacttt catacgcatg attaccagca
     1621 atgggctgtg gttgccaata ttcaaaccag tattgatcag cgtggaattg cctacgagca
     1681 ttttaccttg gatggtcctg ttgctttgtt gccatctggt acgggttatg cgttagtatg
     1741 gactgtgcca gccgagcagg tcgatcgcat tttaaattgg gatgacgcaa cttttctttc
     1801 tgagttgcag gtacattttg gtgatatggc gggcaaattc gtttcatgtg gcaagcgaac
     1861 gggatttcca cttgcactga aatatgcatc acccacaatt gcccatcgaa ctgtattgat
     1921 tggcaatgca tcgcaaacgt tacatccggt tgcagggcag ggttttaatt tgggac
//
LOCUS       DAOQLJ010000274         1967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_40004_length_1967_cov_4.923117, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000274 DAOQLJ010000000
VERSION     DAOQLJ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40004_length_1967_cov_4.923117"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1265)
                     /locus_tag="PK522_11955"
     CDS             complement(111..1265)
                     /locus_tag="PK522_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNC42490.1"
                     /translation="MPAIYFDHNATTAVDEKVLEAMLPFFRQKYGNASSLHSYGIEAR
                     RAINQAREQVAHAVGVQSSQVIFTSGGSEANNLFIRGAADYLKPANIVISAIEHPCVR
                     RPAQELARRSNNPWTLHQLTVNTEGQVNLTDARNVISDKTGLVSVMLANNETGVIQDV
                     AAIAELAKNQGAWMHTDAIQALGKIVLNFADLNVHAMTISAHKIYGPKGAAALIVDKR
                     LILKPLIYGGGHENGMRSGTENVPAIVGFGVACELVKARVSEASTHIARLREYLEQGL
                     SALGATIFGQNVPRLPNTCYFAIPDIEGDTLVVKLDKAGFAVASGAACSSATPGKSHV
                     LEAMGVAPMLARCAVRISLGHDNTLNQIDDFLKAINKIVAALRQFNSMSA"
     gene            complement(1291..1539)
                     /locus_tag="PK522_11960"
     CDS             complement(1291..1539)
                     /locus_tag="PK522_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42491.1"
                     /translation="MKLQHFIVMISLLIFSSSAFAYRCVIDMRKIDEALSKQPAITEM
                     QEQEIRKLRAEGEALHNKGKHKESVEALHKALEMLGVR"
     gene            complement(1891..1966)
                     /locus_tag="PK522_11965"
     tRNA            complement(1891..1966)
                     /locus_tag="PK522_11965"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1931..1933),aa:Met,seq:cat)
BASE COUNT          549 a          444 c          380 g          594 t
ORIGIN      
        1 gtcatgctcc aatctattcg gttatgacta tgaaacactc gggttaaatg gttgctattc
       61 tacttgaaaa aaacaacata accgtaaacg aagtcaatct gttaattcta tcacgcactc
      121 atcgaattaa actggcgtag cgctgcaacg attttattaa tcgcttttag aaaatcatca
      181 atctggttca acgtgttatc atgccccaaa ctaatccgta ccgcgcatcg cgctaacatc
      241 ggcgcaactc ccattgcttc cagtacatga cttttaccag gggtagcact agaacaagca
      301 gcgccactgg caacggcaaa accagcctta tctaacttga cgactagcgt atctccttcg
      361 atatcaggta tagcgaaata acaggtattt ggtaatcggg gaacattttg cccaaaaatc
      421 gttgcaccta atgcgcttaa cccttgctcc aaatattcgc gaagtcgagc aatgtgagta
      481 gaagcttcgc ttactcttgc tttcaccaat tcgcaagcaa cgccaaaacc aacgatcgca
      541 gggacatttt cagttcccga acgcatgcca ttttcgtgcc ccccgccata aattaacggt
      601 tttaggataa ggcgtttatc aacgatgagc gctgccgctc ctttggggcc atagatttta
      661 tgcgctgaga tcgtcattgc atggacatta aggtcagcaa aattaagcac tatcttaccc
      721 aaagcttgta tcgcatcggt atgcatccaa gctccctggt tttttgctag ctcagcaatt
      781 gctgcaacat cttggataac ccctgtttca ttatttgcta gcatgacgga taccaatcct
      841 gtcttgtcgg aaattacatt acgcgcatca gttaaattga cttggccttc tgtattgaca
      901 gttaattgat gtaatgtcca agggttattc gatctgcgcg ctaattcctg ggctggtctc
      961 ctgacgcaag ggtgctcgat ggcgctaatg acgatgttag cgggtttcaa ataatctgct
     1021 gcgccgcgaa taaacaggtt attcgcttcg gaaccgccgc tggtaaaaat aacttgcgaa
     1081 gactgaaccc ctacggcgtg agcaacttgt tcacgtgcct gattgatcgc gcgtcgtgct
     1141 tcaataccgt aactgtgcaa actcgatgca ttcccatact tctgtcggaa aaaaggcagc
     1201 atggcttcca atactttttc atcaaccgca gtcgtcgcgt tgtgatcaaa ataaatggcg
     1261 ggcattggct cgttttatcg ctttaaatat ttaacggaca cctagcattt ctaaagcttt
     1321 atgcagcgct tcgaccgatt ctttatgttt acctttattg tggagcgcct cgccttcagc
     1381 acgtaatttt ctaatttctt gttcttgcat ttcagtgatt gcaggctgtt ttgataaagc
     1441 ttcatcaatt tttcgcatat cgataacaca acgataggca aacgcgctgc tactaaatat
     1501 aagcaagcta atcatgacga taaaatgctg tagtttcatt tgatcctcct atggctttaa
     1561 attagtaact taagtatgac tcgattgatc attaaaagtt agcataagat cttactttac
     1621 agttaagaaa gcagccgtat aaattgtttt atattttttc tgtatacaag cgtcttataa
     1681 ggccaatcgc taaaaatact aaattattcg gaaagccaat tataaaaagg tttttaggct
     1741 ataaatactg aatcactttg gaggttaaat tttttattta aatcccgagt caaaatgaaa
     1801 gattttaaaa cccaaatttg tatttcgtta agaagtgccg atcaagaaca cggttctcag
     1861 ttctttgatc actcttcttt tattttgaat tggtggcgaa tcagggattt gaaccccgga
     1921 ccaacggatt atgattccgc tgctctaacc actgagctaa ttcgcca
//
LOCUS       DAOQLJ010000275         1966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_40045_length_1966_cov_7.100994, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000275 DAOQLJ010000000
VERSION     DAOQLJ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40045_length_1966_cov_7.100994"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1127)
                     /locus_tag="PK522_11970"
     CDS             complement(186..1127)
                     /locus_tag="PK522_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNC42492.1"
                     /translation="MRTAYITHPICLKHDMGYGHPESPLRLQAIEQELRVEGLFPLLR
                     YYEAPLVTHEQLARVHAEHYLHAIETVAPQHGLISIDSDTLMNPYTLEAAKRAAGAAV
                     LATDLVISGQVENAFCNVRPPGHHATRDEAMGFCFFNNVAVGAAHALALHGLTRIAIL
                     DFDVHHGNGTETIFKDEPRIMLCSTFQHPFYPYSGANSSSDHIINTPLAAGTTGQGFR
                     ATVLENWIPALERFKPEMIYISAGFDAHREDEMANLNLVEADYIWVTEQIMTIAERYA
                     QKRIISALEGGYELDALARSVVAHIRVLSAIAEYNSD"
     gene            complement(1151..>1966)
                     /locus_tag="PK522_11975"
     CDS             complement(1151..>1966)
                     /locus_tag="PK522_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNC42493.1"
                     /translation="LALIGSLAICTIFYLFISAGVIGSVGAQPVMDTAGKAFSPGSLA
                     LAEQCQALASMGQHPVVCSREALAHVLRQIGWEKIGNLLGLAAFLALPSVILMMLFGQ
                     TRIFFVMARDGLLPEVLSRIHSRFKTPHIVTLVTGVGVTLAAAFLPVGKLADISNSGT
                     LFAFMVVAITVMILRVRDGNRARPFRTPAIWFVGPLAVIGCITLFLFLPADAKLVFPI
                     WTGIGLVFYFLYGYRKSHVALGTCASAGGENIISPLRSLADGDDKNTNPNNNH"
BASE COUNT          616 a          481 c          400 g          469 t
ORIGIN      
        1 ctaccaactg agctatgccg gctatggaag taaattaaaa caggctgcgt gaacaactat
       61 cactcgacta cttttagaag ctagggttct agctttttaa tactattagc gataaaccgc
      121 ttaattcgcg caagcattct acccgaatat caggcacaaa taaataagcg actcaataaa
      181 aagaattaat ctgaattgta ttcggcaata gcactgagta ctcggatatg agctaccaca
      241 ctcctggcca atgcatccag ctcataccct ccttccaacg ctgaaataat tcgtttttgt
      301 gcatatcttt ctgcgatagt cataatttgc tcggtaaccc aaatataatc cgcttccacc
      361 aagtttagat tagccatttc atcttcgcga tgcgcatcaa aacctgcaga gatataaatc
      421 atctcgggtt taaatctttc taatgctgga atccagttct ctagtaccgt tgcacgaaac
      481 ccttgacctg ttgtaccagc agcaaggggt gtattgatga tatgatcgct gctgctattt
      541 gcaccgctat aaggatagaa aggatgttgg aatgtggaac aaagcattat ccgtggctca
      601 tccttaaata tagtttcagt accgtttcca tgatgcacat caaaatctaa aatagcaatt
      661 cgagtcagcc catgcaaagc caaagcatgc gcagcaccca ctgcaacatt gttaaaaaag
      721 cagaaaccca ttgcctcatc acgggttgca tgatgacctg gaggtcgaac attacaaaat
      781 gcattttcta cctgaccgga tatcaccaga tccgttgcta aaacagcggc gccggctgct
      841 cgcttggctg cctcaagggt ataaggattc atcaaagtat cggaatcgat actaatcaat
      901 ccatgctgcg gtgcaacagt ctcaatcgcg tgaagataat gttctgcatg aacacgggct
      961 aactgctcat gggtcaccag tggcgcttca tagtatcgca ataaaggaaa aagaccttct
     1021 acgcgtaact cctgctcgat cgcttgcaat cgcagaggcg attcaggatg gccataaccc
     1081 atatcatgct tcaaacagat cggatgagta atgtaagcag tcctcatgta cgcagcggtt
     1141 tacccattaa ttagtgatta ttatttggat tggtgttctt atcgtcacca tccgcgagcg
     1201 atcgtaaagg gctaatgata ttttctcctc cagcagaagc acaagtaccc aacgccacat
     1261 ggctttttcg ataaccataa agaaagtaaa aaaccaaacc aattccagtc caaattggaa
     1321 atacaagctt agcatcagcc ggtaagaata agaaaagggt aatgcaacca atgacagcaa
     1381 gtgggccaac aaaccatatt gcaggtgttc tgaaaggtct tgcacggttc ccgtctctga
     1441 cacgcaaaat catgacagtt atcgctacaa ccataaaagc aaagagcgta ccagagttgg
     1501 aaatatcagc gagcttaccc accggtaaga aagccgctgc tagcgtcaca ccaacccccg
     1561 taaccagtgt cacgatgtgg ggtgtcttaa atcgagaatg aattcggctc agtacttcag
     1621 gtaataggcc atcgcgagcc atcacgaaaa aaatccgagt ctgcccaaat agcatcatca
     1681 agatcaccga aggtaatgca agaaacgcgg ccaaaccaag taagttaccg attttttccc
     1741 aaccgatctg tcttaataca tgcgcaagcg cttcacgtga gcaaaccaca ggatgctgcc
     1801 ccattgacgc aagtgcctgg cattgctctg ctaacgcaag cgaaccaggg gaaaaagctt
     1861 taccagcggt atccatcacc ggttgcgcac cgactgagcc aattacaccg gcagaaataa
     1921 ataaataaaa aatcgtacaa atcgcaagcg atccgatcaa tgcaag
//
LOCUS       DAOQLJ010000276         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_42021_length_1915_cov_5.934409, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000276 DAOQLJ010000000
VERSION     DAOQLJ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42021_length_1915_cov_5.934409"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="PK522_11980"
     CDS             complement(<1..164)
                     /locus_tag="PK522_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNC42494.1"
                     /translation="MIETDRIISAAPSSQKEEVMERALRPKQLEEYIGQEKIREQLQI
                     FIEAARRRNES"
     gene            complement(195..776)
                     /gene="ruvA"
                     /locus_tag="PK522_11985"
     CDS             complement(195..776)
                     /gene="ruvA"
                     /locus_tag="PK522_11985"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNC42495.1"
                     /translation="MIAKISGVLLEKHPPQVLVNVHGIGYEIDVPMSTFYQLPTIGEA
                     VMLHTHFVIREDAHLLFGFSTIEERAAFRQLIKISGIGARTALALLSGLSVLDLQETV
                     VNQDADRLIKVPGIGKKTAERLLLELRDKLNIEVQPCSSDSPETSNNHDILNALFSLG
                     YNEREIGWVIKQIPKGTTVSEGIKQALKLLSKK"
     gene            complement(789..1280)
                     /gene="ruvC"
                     /locus_tag="PK522_11990"
     CDS             complement(789..1280)
                     /gene="ruvC"
                     /locus_tag="PK522_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNC42496.1"
                     /translation="MRILGIDPGLRITGFGVIDKVGNKLTYVSSGCIKTSGDQLPQRL
                     KIILDSLKMIICEHEPDQVAIEHVFVNINPKSTLLLGQARGAAICAAVIHNLMVTEYT
                     ALQIKQAVVGNGHAAKEQVQEMIIRLLNLGGRPGADAADALACAICHAHSLVNNRSNL
                     VLR"
     gene            complement(1307..>1915)
                     /locus_tag="PK522_11995"
     CDS             complement(1307..>1915)
                     /locus_tag="PK522_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNC42497.1"
                     /translation="GGDSSSNPRLRLAIDKAYEQNMPKDNIERAIKRGCGDLEGVSYE
                     EIRYEGYGIGGAAIMVDCMTDNRTRTVADVRHAFTKYGGNLGTDGSVAFLFNHCGQFL
                     YPPGTDENKLIETALEVGAEDIIHHEDDSLEVVTAPYEFITVKTGLEKAGFKAEFAEV
                     TMKPTNETLLSGEDAIKMQKLLDALENIDDVQDVYTTAVLAE"
BASE COUNT          507 a          486 c          343 g          579 t
ORIGIN      
        1 gattcatttc ggcggcgagc tgcttcaata aaaatctgca attgctccct gatcttttct
       61 tggccaatat actcttcaag ctgttttgga cgcagagccc gctccattac ttcctctttt
      121 tgggaagaag gagctgctga aatgatacga tccgtttcta tcactgttgt tctattggag
      181 ctaccagatg gtcattattt ttttgaaagc agtttcagcg cttgcttgat accttccgaa
      241 actgtcgtac ctttaggaat ttgcttaatt acccagccta tttctcgttc attataacct
      301 aacgagaata gcgcattgag tatgtcatga ttgttgctcg tttcagggga atcgctagaa
      361 cagggttgca cctctatatt cagtttatcc cgcaattcaa gcaatagcct ttctgcagtt
      421 ttcttaccaa tacctggaac cttgatcaat ctatcagcat cttgattgac gaccgtctct
      481 tgtaaatcga gcacgcttaa cccagatagc agtgcgagcg ccgttcttgc accaatacct
      541 gaaattttga ttaattggcg aaaagcagct cgctcctcaa tggtagaaaa accaaacaac
      601 aaatgagcat cttcgcgaat cacgaaatgc gtatgaagca tgactgcctc accaatggtg
      661 ggaagctgat aaaacgtact cataggcaca tcgatttcat aaccgatccc atgcacatta
      721 actaatacct gcggaggatg tttctcaagc agcacacccg atattttcgc tatcatgttt
      781 aaatcatact acctgagaac aagattagaa cgattattca ctaaactatg tgcatgacaa
      841 attgcgcaag ccaatgcgtc tgctgcatca gcgccaggtc taccacctag atttaataat
      901 cgtataatca tttcttggac ttgttctttt gcagcgtggc cattccccac aaccgcttgc
      961 tttatttgta atgccgtata ctccgtcacc ataagattat gaatgactgc cgcgcaaata
     1021 gcagcgcccc gcgcttgacc cagcaataaa gtcgatttgg gattgatatt aacgaaaaca
     1081 tgctcgatcg caacctgatc tggctcatgc tcacaaatga tcattttaag gctatcgaga
     1141 attatcttta aacgctgcgg caattgatcg cccgatgtct taatgcaccc actactgaca
     1201 taggttagct tattgccaac cttatcaatc acaccaaacc ctgtgatacg taaccctggg
     1261 tctatgccaa gaatacgaat ttctccccct ttttatcgca agtaatctat tccgctaaaa
     1321 ctgcagtcgt atagacatct tgaacatcat caatgttttc taaagcatcc aatagtttct
     1381 gcattttgat tgcgtcttca cccgacaata acgtttcatt cgtcggcttc atcgttacct
     1441 ctgcaaattc tgctttaaac cctgcctttt ctaatccagt tttaacggtg ataaactcat
     1501 acggcgcagt cacaacctct aggctatcgt cttcatgatg gatgatatct tctgcgccga
     1561 cttccagtgc tgtttcgatc aatttatttt cgtcggttcc tggtggataa agaaattggc
     1621 cacagtggtt aaataaaaaa gcaaccgatc catccgtccc gagattgcca ccatactttg
     1681 taaaagcatg gcgcacatct gctacagtcc tcgttctatt atcggtcatg cagtcaacca
     1741 taatcgcggc cccacctatt ccatagccct catatcgaat ttcctcatag ctaacgcctt
     1801 ccagatcacc acatcctcgt ttaatcgctc tctcgatatt gtctttgggc atattttgct
     1861 catatgcttt atcaatcgct aaccgtaagc gtggattact gcttgagtct cctcc
//
LOCUS       DAOQLJ010000277         1905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_42432_length_1905_cov_7.378378, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000277 DAOQLJ010000000
VERSION     DAOQLJ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42432_length_1905_cov_7.378378"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /gene="argA"
                     /locus_tag="PK522_12000"
     CDS             complement(<1..819)
                     /gene="argA"
                     /locus_tag="PK522_12000"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HNC42498.1"
                     /translation="MKKNFTPPSISVDLKNEDLHTYFIAWFRSVAPYIHTFRGKIFVV
                     AFGGEVVADGKFVELVHDLNLLASLGIQLVLVHGARPQIESRLQRDNLDITYVNGIRA
                     TDAVTLERVIESVGRVRMEIEALMSMGLPNSPMANASIRVTSGNFVIARPIGVMNGTD
                     LMYTGEVRKINLTAIHSHLTQGEIVLISPLGYSPTGEIFNLTLENVAAEVAITLKAEK
                     LIFLMNTPGIMDQSTKQPDNLLRELTVAESKSYLTQLADLPTHIPDDTQFYLPQA"
     gene            1101..>1905
                     /locus_tag="PK522_12005"
     CDS             1101..>1905
                     /locus_tag="PK522_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNC42499.1"
                     /translation="MIFWRVKSLDTILATAEKKSLNRTLGVWQLTLLGVGAIIGTGIF
                     VLTAEAAQKAGPGMMFAFIIAGFVCAVAALCYSELSSMVPVSGSAYTYSYAVLGEFVA
                     WIVGWGLILEYSVAAGAVAVGWSGYFVGLLSNSLGIEIPFMLANGPFAGGIINLPAVV
                     ICMLVIGLLVVGTKESATFNAVLVAVKITALTIFIALALPVSDMENFQPFLPLGSTGV
                     VAAAASIFFAYVGFDAVSTAAEETKNPQRNVPLALIGSLAICTIFYLFIS"
BASE COUNT          480 a          369 c          469 g          587 t
ORIGIN      
        1 ggcttgtggt aaatagaact gagtatcatc cggaatatgg gtgggaaggt cagccaattg
       61 ggttagatag gatttgcttt ctgctaccgt tagctcgcgt aatagattat ctggctgctt
      121 tgtggactga tccataatgc ctggcgtatt catcaaaaaa atcaatttct ctgcttttag
      181 cgtgatggcg acttctgccg caacattttc aagcgttaga ttaaaaattt ctccggtagg
      241 tgaataaccg agtggtgaaa ttaaaacaat ttccccttga gttaggtgag aatggatagc
      301 ggttaaattt attttccgga cttcgccggt atacatcaag tcagtcccat tcatcacgcc
      361 aatcggtcga gcaattacaa aattaccgct ggtaactcgg atagaagcgt ttgccatggg
      421 agaattaggg agacccatgg acattaaagc ttcaatttcc atgcgtacac gtcctactga
      481 ttcaatgaca cgttcaagcg ttacagcatc ggtagcgcgg ataccattaa cgtaggtaat
      541 atcaagatta tctcgctgca atcttgattc gatctggggg cgcgcaccat gtacaagcac
      601 aagctggatt cccaggcttg ccaataaatt tagatcatgc accagctcga caaattttcc
      661 atccgcaacc acttctccgc cgaaggcaac cacaaaaatt ttaccgcgga aagtgtggat
      721 gtaaggtgct actgaacgga accaggcaat gaaataggtg tgcaaatctt cattttttag
      781 atcgactgat attgagggag gggtgaaatt ttttttcatg cgtgtaatgg gtaaatcaat
      841 ttcagcgatt tcattctgac caaagatttt caaaaaaacg gctggattta gctaagttaa
      901 gtatatcgac taagttagct aggaagtatt tcacaagctg caagattatt caaacgtgct
      961 tgagggtagt cacgaatcga aaatgaatcg attagttgcg ttgcagacta tttttttgga
     1021 gcttgctcgc cacttatttc ttgtaaacta ttccctaaat gaatgttgaa taatgattgt
     1081 tggaattaaa ggggaaatcg atgattttct ggcgagtaaa atcactagat acgatcttgg
     1141 cgacagcaga aaaaaaatcg ctcaatcgga cgctgggtgt gtggcaacta acacttttgg
     1201 gtgtcggtgc tattattggc actgggatat ttgtattaac cgctgaggcg gctcagaaag
     1261 caggccctgg catgatgttt gcgtttatca ttgcaggttt cgtgtgtgca gttgcggcat
     1321 tatgctattc agagctttct tcgatggtac cggtttcggg ttctgcttat acctactctt
     1381 atgctgtact cggtgagttt gttgcgtgga ttgtcggctg ggggttaatt cttgaatact
     1441 cagttgcggc tggcgctgtt gccgtagggt ggtcagggta tttcgttggg ttgctgagca
     1501 actcattagg gattgagatt ccattcatgc tggcaaatgg accttttgcg ggtgggatta
     1561 ttaatttgcc agccgtggtg atttgtatgc tggtgatcgg tttgctggtc gtcggtacta
     1621 aagagagtgc tacctttaat gcagtgttgg ttgcagtcaa gattaccgcg ctcacgatat
     1681 tcattgcgct tgccttgcca gtttccgata tggaaaattt tcaaccattt cttcccttgg
     1741 ggtcgacggg tgtcgttgcc gcagccgcgt ctatcttctt tgcttacgta gggtttgatg
     1801 cagtatcaac cgcagcggaa gaaacaaaga atccacagcg caatgttcca cttgcattga
     1861 tcggatcgct tgcgatttgt acgatttttt atttatttat ttctg
//
LOCUS       DAOQLJ010000278         1850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_44865_length_1850_cov_5.788858, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000278 DAOQLJ010000000
VERSION     DAOQLJ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44865_length_1850_cov_5.788858"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PK522_12010"
     CDS             complement(<1..628)
                     /locus_tag="PK522_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNC42500.1"
                     /translation="MDDKPKRTWDEAAYKWLMETEHKASHQQDMKQVAWIQPYFRGRF
                     LSELTREVIAEVGEIKRKETSPATANRYLATIRAILRRAALDWEWIDKPPVIKLYREA
                     KRRVRYLNATQASILIRELPPHLADMVTFSLATGLRRSNVTKLEWSQVDMQRNVAWIH
                     GDQAKAGKPIHVTLNATAMAVLTRQIGKHPRSVFSYKGKPITQVNTKAW"
     gene            complement(655..984)
                     /locus_tag="PK522_12015"
     CDS             complement(655..984)
                     /locus_tag="PK522_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC42501.1"
                     /translation="MKTLNLTEAAQFLKLHPEEVRRRAKAGIIPGAKLGKRWAFIEDD
                     LADHIRSLYACTRQALQVGHEEKKLCHSLNVVKRGGFDSLHQAASALDALLQQKIKPK
                     PKNSTTG"
     gene            complement(1010..1438)
                     /locus_tag="PK522_12020"
     CDS             complement(1010..1438)
                     /locus_tag="PK522_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraM"
                     /protein_id="HNC42502.1"
                     /translation="MSNNRLDALIERVASEHGIVLSQDDPVLMMHTLNEVLLEQNEKA
                     HTELLSNYRAILEENFNRWCEFSTKKSNAIISASMNNAQLTRDQFLESCIQLIDERIK
                     SSINQETYDLVRISRQMAIMNLLAAVLVLVSVVIVVFMLL"
     gene            complement(1435..>1850)
                     /locus_tag="PK522_12025"
     CDS             complement(1435..>1850)
                     /locus_tag="PK522_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011304098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="HNC42503.1"
                     /translation="ALLLDMGHELVVHTVITGSQALLDTLNGFVHLIKQFPKEALFVV
                     WLNPYWGEISMNGKQFEEMKAYVDNKTRVSAIIRIPHYKAETFGKDLSEILQSKFTFD
                     EALKNSALPVMVRQRLTIIRRQLFTNIENLAAVLT"
BASE COUNT          476 a          431 c          364 g          579 t
ORIGIN      
        1 accaagcttt tgtatttact tgcgtgattg gcttgccctt gtaactaaat accgatctgg
       61 gatgcttgcc aatctgccta gtcaataccg ccattgccgt agcatttaac gttacatgaa
      121 tcggttttcc cgccttggcc tgatcgccat gtatccatgc aacatttctt tgcatatcca
      181 cttgcgacca ttccagcttt gtaacgtttg atcgtctcaa accagtcgct agtgaaaacg
      241 tcaccatgtc cgctagatgc ggtggtaatt cccgaatgag aatgcttgct tgtgtcgcat
      301 tcaagtaacg cactcgacgt tttgcctcac gatacaactt aatgactgga ggtttatcga
      361 tccattccca atctaatgca gcccgccgaa gaatcgcccg aattgttgcc aggtagcgat
      421 tagccgttgc aggtgaagtt tccttacgtt taatttcacc cacttcagca atcacttctc
      481 gggttaattc ggaaaggaaa cggcccctaa aatagggctg aatccaggca acttgcttca
      541 tatcctgctg atgtgaagct ttatgttctg tttccattaa ccatttatag gcggcctcat
      601 cccaagtccg cttgggctta tcatccaatt tagtaactcg ccacgattca gcttttagcc
      661 tgtcgtggaa ttcttgggct tgggttttat cttctgttgc agcagagcat ctaatgcgct
      721 cgccgcttgg tgtagtgaat cgaatccacc acgttttacc acgtttaaag agtgacataa
      781 ttttttctcc tcatgtccca cttgcaacgc ttgccgggta caagcatata acgagcggat
      841 atgatcggca aggtcatctt caataaatgc ccagcgtttt cctaatttgg caccgggaat
      901 gattccggct ttggcgcgac ggcgcacttc ttcgggatgc aatttcagaa actgggctgc
      961 ttcggtgagg tttaaagttt tcataagcta tacaaccttt tagtagaagc tataacagca
     1021 tgaatacaac aattaccacg gaaactagaa ccagaacggc agccaataaa ttcatgatag
     1081 ccatttgcct ggaaattctg actagatcat aagtttcctg atttatactg ctcttaattc
     1141 tttcatcgat aagctgaata cagctttcca ggaattggtc tcgtgttaat tgcgcgttat
     1201 tcattgatgc actgatgatg gcatttgatt ttttggtaga gaactcacac catcgattaa
     1261 agttttcttc taatatcgcc cggtaattgc ttagcagctc agtatgggct ttctcatttt
     1321 gttccagtaa tacctcattt aaggtatgca tcatcaatac tggatcatct tgcgacagaa
     1381 ctattccatg ttctgaagct actcgttcaa tgagcgcatc taatctgtta ttgctcatgt
     1441 taaaaccgca gctaaatttt caatgttggt gaagagctgc ctgcggataa tcgttaacct
     1501 ttgacgcacc atcaccggta atgcgctatt tttaagcgct tcatcaaagg taaattttga
     1561 ttgcaagatt tcacttaaat ctttaccaaa agtttctgcc ttataatgtg gaattctgat
     1621 gatagctgat actcgggttt tgttatctac gtacgctttc atttcctcaa attgtttgcc
     1681 attcatactg atttctcccc agtagggatt taaccaaacg acaaacaatg cttctttcgg
     1741 aaactgcttg atcaaatgca caaaaccatt caatgtgtcc aacaaagctt gactgccggt
     1801 gatgacagta tgcacaacca gttcatgccc catatctaaa agtaaggcgg
//
LOCUS       DAOQLJ010000279         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_45136_length_1844_cov_5.675238, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000279 DAOQLJ010000000
VERSION     DAOQLJ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45136_length_1844_cov_5.675238"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..1624)
                     /gene="sbcB"
                     /locus_tag="PK522_12030"
     CDS             complement(179..1624)
                     /gene="sbcB"
                     /locus_tag="PK522_12030"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HNC42504.1"
                     /translation="MPTSLYWHDYETTGSDPHCDRPIQFAGVRTDESLQEISEPLVIY
                     CAPPRDRLPQPEACLLTGITPQFADEHGLIEFEFITKIHQALAQPNTCGVGYNSLRFD
                     DEVTRFTLYRNFYDPYAREWQQGNSRWDLIDVVRMTYALRPDGIIWPINEAGSPIFRL
                     EDLTRSNHLTHDSAHDALSDVRATIQLARLIRDRQPRLYNWLFELRDKHKVIPLLNSH
                     DHTPIVHTSRMYPAEIGCTTLVMPIGQDPRNSNSVLVYDLRYDPSAFLRMTIDELSHH
                     LFTPRSALPENSIRLPIKAIRVNKCPAIAPRSVLNDESIARIKLDLPACNQYWQIIKD
                     DKTNFMKKMVNAYSRTAFEEATDVELALYDNFFSSNDQNLIKMVRSTPPTELSNKYFQ
                     FNDKRLPELLFRFRARNWPETLTAEELECWKIHCYDYLTNKLNPNNLTISEYNETITL
                     LRGVYQENIIANDILDKIEIWGKNLVKEVQC"
     gene            complement(1629..>1844)
                     /locus_tag="PK522_12035"
     CDS             complement(1629..>1844)
                     /locus_tag="PK522_12035"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNC42505.1"
                     /translation="TCGAEIPRWIRKKLEGYGDDSASITAFGLDVVTELCDRLLKAGA
                     PGLHFYTLNSATLTTKIWQRLFSNLSS"
BASE COUNT          531 a          350 c          385 g          578 t
ORIGIN      
        1 taatcagcca aatactgtat gaagttattg caacagtcga gcagcctggt attaaatttg
       61 gtgatatttt caatatcaaa cttaagggaa aacgaaaaga gttaaccgtt cgctatgttc
      121 attcagatga tcactgaaaa gaatgtgact aaattttctc ctcattacag ttttatgatc
      181 agcattgcac ttctttaacc aaattctttc cccaaatctc aatcttatca agtatgtcgt
      241 ttgcgatgat attttcttga taaacaccgc gaagcagggt tatcgtttcg ttatattcag
      301 aaatcgttag attatttgga ttcagcttgt tagtgagata gtcatagcaa tggattttcc
      361 aacactctaa ttcttcagcc gttagcgttt ctggccaatt gcgagctcgg aaacggaata
      421 atagttctgg taatcgctta tcgttaaatt gaaagtattt gttgctgagt tcagtaggtg
      481 gagtgcttcg caccattttt atcaaattct gatcgttact agaaaaaaag ttgtcataaa
      541 gtgcgagctc aacatcggta gcttcttcaa atgctgttct actataggca ttcaccattt
      601 ttttcataaa atttgttttg tcatctttga taatttgcca atattgatta caagcaggta
      661 aatccagttt aattcgcgca atactttcat cattgagtac acttctagga gcgatggcag
      721 ggcatttatt cactcttatt gctttgattg gtaaacggat actgttctca ggtaaagcac
      781 tacggggagt aaatagatgg tgtgagagtt catcgattgt catgcggagg aaggcgcttg
      841 gatcatagcg taaatcatat actaaaacgc tattactatt tctcgggtct tgcccaattg
      901 gcatgaccag tgttgtgcat ccgatttcag ccggatacat tcgagaagta tgaacaattg
      961 gcgtatgatc gtgtgaatta agtagaggga taactttatg tttatctctt aattcaaaaa
     1021 gccagttata tagacgtggc tgtcgatctc gaatcaggcg agcaagctgt atggttgctc
     1081 taacatctga taatgcatca tgagcagaat catgcgtaag gtgattagat ctcgttaaat
     1141 cctctagtcg gaaaattggg ctaccagctt cattgattgg ccaaatgatc ccatccggtc
     1201 taagcgcata ggtcattcgt acgacatcaa tgagatccca acgcgagtta ccctgctgcc
     1261 attcccgtgc ataaggatca tagaaattac gatataacgt aaagcgtgtt acttcatcat
     1321 cgaatcttaa cgagttgtaa ccaactccac atgtgttcgg ctgtgctaac gcttggtgaa
     1381 ttttggtaat aaattcaaat tcgattaaac cgtgctcatc agcaaactga ggtgtaattc
     1441 ccgttaacaa acaagcttct ggctggggta accgatctcg gggaggagcg cagtaaataa
     1501 cgagtggttc acttatttct tgtagtgatt cgtcagttct tacaccagca aattggatag
     1561 ggcgatcaca atggggatct gagccagttg tttcataatc atgccaatat agcgatgttg
     1621 gcattccatt aagaagataa gtttgagaac aatcgttgcc agattttagt cgttaacgtt
     1681 gctgaattta atgtatagaa atgaaggcca ggtgcaccag ccttgagtag gcgatcacat
     1741 aattcagtga ctacatctaa tccaaaagct gtgattgacg cagagtcatc gccatatcct
     1801 tccaattttt ttctaatcca gcggggaatt tctgcgccac aggt
//
LOCUS       DAOQLJ010000280         1812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_46660_length_1812_cov_4.286853, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000280 DAOQLJ010000000
VERSION     DAOQLJ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46660_length_1812_cov_4.286853"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PK522_12040"
     CDS             <1..286
                     /locus_tag="PK522_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNC42506.1"
                     /translation="QEAVITYMLEKVMTKRRILEIYLNMIEWGENVFGAEAASQHYFG
                     VKASALTPRQAAFLASMVPNPRFYDRNRTAKKLLKKTDIILKRMPSSQIP"
     gene            476..>1812
                     /gene="mgtE"
                     /locus_tag="PK522_12045"
     CDS             476..>1812
                     /gene="mgtE"
                     /locus_tag="PK522_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNC42507.1"
                     /translation="MMDNFDNIELQNKLERVINLLRKYRLVEGLVQMQDSPRQELIES
                     VTQKQNLASLQKLIDTLHPADIAYILESLPLEDRLFLWKFVKVENDGEVLLEVSDAVR
                     ETLIADMGSNELVAAAEQLDADEIADIAPNLPDDVIEDVYRALPQEEREQLRAAMSYP
                     EDSVGALMDFDIITIREDVRLEVVLRYLRFLGELPDHTDQLFVVDRNELLQGVLMLNQ
                     LLVNDPETMVSNVMTKEGVKFHPHDNAQQATQAFERYDLVSASVVDADGKLLGRVTVN
                     AVIDFIRNKAESQARNLAGLSEQEDLFAPIWKSVRNRWAWLAINLVTAFIASRVIGLF
                     EDSIEKLVALAALMPIVAGVGGNSGNQTITMIVRALALGQVSTENTVKLITKEIGVSL
                     VNGLLWGGIVGLFTFLIYQNASLGLVMALAMILNLLLAALVGVIIPLTRKKLGR"
BASE COUNT          506 a          387 c          406 g          513 t
ORIGIN      
        1 acaagaagcg gttattactt acatgctaga aaaagtaatg acaaagaggc gcatcttaga
       61 aatttatttg aatatgatcg aatggggtga aaatgtattt ggtgctgaag cagcttccca
      121 gcattatttt ggggttaagg cttcggcatt aactcccaga caggctgcct tccttgcatc
      181 catggtccct aatccacgtt tttatgatcg taatcgcact gcaaaaaaac tgttaaaaaa
      241 aacggatatc atcttaaaac gtatgccttc ttctcagatc ccctaaaaaa ttacttaata
      301 ctctttttcc acacaattga atagccttga ttttgttatc ttctaatctt atcttctaat
      361 cttatcttcg atacgatgca gcatgttaaa ttagagaatt tcaggctaag caatttttta
      421 atcatccaat ccactcaatc aggggtagca acttcttcaa taggcatggc atgagatgat
      481 ggataatttt gacaatatag agctacaaaa caagcttgag cgcgtcatca acttactgcg
      541 taagtaccga ctggtcgaag gcttggtgca aatgcaagac tctccacgcc aagaattgat
      601 tgagtcggtc acgcagaaac aaaacctggc aagcctacaa aaattaatcg ataccctgca
      661 tcctgctgat atcgcttata ttttagaaag cctgccactg gaagatcggt tattcctttg
      721 gaaatttgtt aaagtcgaga atgatggcga agtactcctc gaagtatccg atgccgtaag
      781 agaaacgcta attgctgata tgggaagcaa cgaattggtt gcggccgccg agcaattaga
      841 cgccgacgaa attgccgata tcgcacccaa tttaccggat gacgtcattg aggatgttta
      901 tcgcgcactt cctcaggaag agcgtgagca actccgtgca gcgatgtcct atccagaaga
      961 ttctgttgga gcactcatgg atttcgatat catcacgatc cgtgaagatg ttcgattaga
     1021 agtcgtgtta cgttacctac gctttctcgg tgaattaccc gatcataccg atcagttatt
     1081 cgtcgtcgat cggaatgaat tgctacaggg cgttctcatg ctcaatcaat tgttagtcaa
     1141 tgatcccgaa acgatggtca gtaatgtcat gactaaagaa ggtgtaaaat tccaccccca
     1201 tgataatgcg cagcaagcca cgcaagcatt tgaacgctat gatttggttt cggcttccgt
     1261 cgtcgacgca gatggaaaac tgttgggccg tgttactgtt aatgctgtga tcgatttcat
     1321 tcgaaataaa gcagagagtc aagcgcgcaa tcttgcggga ttgagcgagc aagaggattt
     1381 atttgccccc atttggaaaa gtgtgcgtaa tcgatgggcg tggctcgcaa ttaatttggt
     1441 tactgcattt attgcatcgc gcgtcattgg cctatttgaa gattcaatcg agaagctagt
     1501 cgcattagcc gccttaatgc cgattgtggc tggcgttggg ggtaactccg gtaatcaaac
     1561 catcacgatg atcgtgcgtg cgttagcgct aggtcaagtc agtactgaga ataccgtcaa
     1621 actcatcacc aaagaaattg gggtcagctt ggtaaatggc cttctatggg gaggaatcgt
     1681 cggtttattt acgtttctga tttatcagaa tgcctccctt ggtctcgtta tggcactagc
     1741 gatgatattg aatcttttac tcgcggcact agtcggcgtc atcattccgc tgactcgcaa
     1801 aaaattaggc cg
//
LOCUS       DAOQLJ010000281         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_47203_length_1801_cov_7.132302, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000281 DAOQLJ010000000
VERSION     DAOQLJ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47203_length_1801_cov_7.132302"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..310)
                     /locus_tag="PK522_12050"
     CDS             complement(62..310)
                     /locus_tag="PK522_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42508.1"
                     /translation="MLRPTKHSHPDRTVINISLLLLQRLQARRFDEYDALKKYAKKAV
                     VGGDILFLPALNFLYLLGLIDYHPKTDAVEYIGPNEAI"
     gene            complement(303..1253)
                     /locus_tag="PK522_12055"
     CDS             complement(303..1253)
                     /locus_tag="PK522_12055"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNC42509.1"
                     /translation="MKYAYEDLNSDQFETLIVFLCQHLLGISVQGFSRGPDGGRDAKF
                     VGTAELHPSKASPWIGTIIIQAKHTNGYNRHFSEADFSGGVNSIVGKEIQRIKKLRHS
                     KQLDHYMLFANRRLAGNAESDIRAQISKECDIPESSIYLCGIEQLEIFLKTFPEVALK
                     ANLDPVDSPLIVSSDDLAVVVQALAKYRTALSAVIDTPPVPRIPYERKNELNNMTEAY
                     AKEQRRRYLKDTSQIRTFLAAPENIDLQRLYESVTEEFQLKITSKRKDHQSFDCVMEY
                     LVELLFSRDPILCQLAHKRLTRAVLFYMYWNCDIGELDDA"
     gene            complement(1500..>1801)
                     /locus_tag="PK522_12060"
     CDS             complement(1500..>1801)
                     /locus_tag="PK522_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HNC42510.1"
                     /translation="EWETIGTIKKVDEGIKPGVYNIYLATAPVDKNQYQGQIIYIDKE
                     NAVFYQQVNKDYIVHQLNAVDDKPVAGKDVAITYDGKKATLTLIDALKNKRSLKI"
BASE COUNT          509 a          379 c          357 g          556 t
ORIGIN      
        1 aacattcatg cccgcacgaa aatcaaccgc ctcaaaaaga tcagacttat tagaataaaa
       61 tctagatagc ttcattaggc cctatatact ccacagcatc tgtcttgggg tgatagtcga
      121 tcaaacccag cagatacaaa aaatttaatg cgggtaagaa aagtatgtcg cctccaacaa
      181 cagccttctt cgcatatttc ttcaaagcgt catactcgtc aaagcggcga gcttgcaaac
      241 gttgcaataa aagcaaggat atattgatga ctgtgcgatc tgggtgagaa tgtttagttg
      301 gtctaagcat cgtctaattc tccaatgtcg caattccagt acatatagaa tagtactgct
      361 cgtgttagtc gcttgtgtgc aagctgacac agtattggat cgcgactaaa tagcaattca
      421 acaagatact ccattacaca atcaaaggac tggtgatcct tgcgtttgga agtgatttta
      481 agttgaaact cctctgtcac tgattcatat aagcgttgta aatcaatgtt ctcaggcgca
      541 gctaagaagg ttctaatctg ggatgtgtct ttgaggtagc gtctacgttg ttctttggcg
      601 taagcctctg tcatgttatt aagctcattt ttccgctcgt agggaatgcg tggtacaggt
      661 ggcgtgtcaa ttacagcaga caaagcagtg cgatactttg ccagcgcttg tactaccaca
      721 gctaaatcgt ccgagctaac aattaacggg gagtcaactg gatcaagatt tgccttaagc
      781 gcgacttcag ggaaagtttt tagaaatatt tccaattgct caatgccgca caggtagatt
      841 gagctctcgg gaatgtcaca ttcttttgaa atttgagcac gtatatcgct ctctgcgttg
      901 ccagctaagc gccgatttgc gaatagcatg taatgatcaa gttgtttgct atgacgtagt
      961 ttcttaattc gttgaatttc tttaccgact attgagttta caccaccgct aaagtcagct
     1021 tcagaaaaat gacggttata gccgttggta tgcttagctt ggataattat tgttccgatc
     1081 caaggcgaag ctttactcgg gtgcagttct gcggttccaa caaatttagc atcgcgacct
     1141 ccatctggtc ctcttgaaaa tccctgaacc gatataccaa gcaggtgctg gcacaaaaat
     1201 acaatgagtg tctcgaattg atcgctattc agatcttcgt aggcatattt catttgaatt
     1261 ccgtgagggt atctatgtga tatttaccag attcaacatc atttatagtg ttgcattgta
     1321 tcagtctcat cgcgcaatca ccacgtacta atggttaatg aacccaaatc catcaatgca
     1381 tagatagctc agactgaggt atgttggttt tcatagaatt cttgatacaa tcacttcaaa
     1441 gcagtacatg acattgttga ctggtaaatc ttcagttctg attggcaaaa tttatccgat
     1501 caaattttca acgatctttt attcttaagc gcatctatta gagttaacgt agctttcttc
     1561 ccatcatagg tgatagccac atctttcccc gcaacaggtt tgtcatcaac agcatttaat
     1621 tggtgcacaa tataatcctt gttgacctgc tgataaaaaa cagcattctc tttatcgata
     1681 taaataattt gtccttgata ctggttttta tcaacgggtg ctgtagctaa ataaatattg
     1741 tagacacccg gcttgatacc ttcatcaacc ttcttgatgg tgccaatcgt ttcccattca
     1801 t
//
LOCUS       DAOQLJ010000282         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_47466_length_1796_cov_5.775991, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000282 DAOQLJ010000000
VERSION     DAOQLJ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47466_length_1796_cov_5.775991"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="PK522_12065"
     CDS             <1..1118
                     /locus_tag="PK522_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42511.1"
                     /translation="KLGAGFRGTGLWVENQNSGNLGGGFNVDNARLYLNGQFHQYLKF
                     EVNTECYFCDNTASKDNPKMSYNILDAIAKFEFNRYFNVWGGRMLAPTERGELNGPFF
                     HATHDGFKTPFFSQDFSTKYGSGGAGMYGRDDGATLWGSVEPGIVPGTFSYAMGIFRG
                     LRSSTGEGPNPGGNVLTAARFTYNFLNPEKNPGYYTAGTYYGKAGDILALAFGMTYQK
                     DGAGSLAHRSDFLGFVGDMLFEKVLPQNMGVLTANAEYKQFYANYSSAAFNDKDCFCM
                     FDGKSWTVTGLYLIPTKIGIGQFQPYGRYTSVQPNRSSNRDEIEAGLNYIIDGFNARI
                     SAYYQHGDLFTRGLNYAPTGISGRAVDVFKISFQLQI"
     gene            1163..>1796
                     /locus_tag="PK522_12070"
     CDS             1163..>1796
                     /locus_tag="PK522_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HNC42512.1"
                     /translation="MQFSTQTGVPLTHQHNSNVLTYPSGKQSAEHQPKSVMKPVHNPL
                     QARLSMRFGHFDGVTRLVEREHYGPLLIQKPLYPEGRGICHAVIIHPPGGVVGGDELA
                     IAARVDASAKAQITTPGAAKWYKANGFTSHQEININVAKGGALEWVPQETIFYNHSNV
                     KIDHQVALEDDAVYVGCEILCFGRTAYGETFDEGQIKQRTRIIRNGKMIWL"
BASE COUNT          480 a          364 c          448 g          504 t
ORIGIN      
        1 taaaactggg tgccggtttt cgtggaactg ggctatgggt tgaaaaccaa aactcaggca
       61 atcttggcgg gggattcaac gtagacaatg cccgtcttta tttgaatggg cagtttcacc
      121 agtatttgaa gttcgaagtg aacacagagt gttatttctg cgataacaca gcttcaaagg
      181 ataatcctaa aatgtcttac aacattttag atgcgatcgc taaattcgag tttaatcgtt
      241 attttaatgt ttggggaggc cgaatgctgg ccccaaccga acggggtgag ttgaatgggc
      301 cttttttcca tgcaacccat gacggtttta aaacaccatt cttctcccag gatttcagta
      361 caaagtatgg atcgggtgga gccggtatgt atggtcgtga cgatggtgcg actttatggg
      421 gaagtgttga accgggaatc gttccaggaa cgtttagcta tgcgatgggt atcttccgtg
      481 gactccgatc ttcgacagga gaaggaccca atccaggtgg caatgtttta acggcagccc
      541 ggtttactta taactttcta aatccagaga aaaatcccgg ttattacacg gccggtactt
      601 actatggtaa agcgggcgat attttggcgc ttgctttcgg tatgacctat caaaaagatg
      661 gcgcaggttc tctcgcgcat cgtagtgatt tcttaggttt tgtgggtgat atgttgttcg
      721 agaaagtatt gcctcagaat atgggggtgc taacggctaa cgcagaatac aagcaatttt
      781 acgcaaacta ttcatcagcc gcatttaacg acaaagattg tttttgtatg tttgatggta
      841 aatcatggac tgtaaccggt ctttatttga ttccgaccaa aattggtatt ggacagttcc
      901 aaccctacgg tagatatacc agcgtgcagc ctaaccgcag cagtaaccgc gatgaaatcg
      961 aagcagggct gaattacatc atcgatgggt ttaacgcccg tatctcagcg tattatcaac
     1021 atggtgacct cttcacgaga ggactgaatt atgcacctac gggtatatca ggacgagcag
     1081 tcgatgtgtt caagatttca ttccagctac agatttgagg ttttctcgtt attgcttagt
     1141 caatttacaa gaaatttaaa taatgcaatt ttcaacacaa acaggggtac cgttgactca
     1201 tcagcataat tcaaatgtgt taacttatcc cagtggcaag cagagcgctg agcaccagcc
     1261 taagtctgta atgaaacctg tccataatcc attacaagcc agattatcga tgcgttttgg
     1321 tcattttgat ggtgtcacac ggttagtcga gcgtgaacat tatgggccgc tgcttatcca
     1381 gaagccactc tatcccgaag gacgtggcat ttgccacgcg gtcattattc atccgcccgg
     1441 tggggtagtg gggggggatg agctagcaat tgctgctcgc gtcgatgcct ctgcgaaagc
     1501 ccaaattacg acgcctggtg ccgcaaaatg gtacaaagcc aatggcttta cttctcatca
     1561 ggagattaac atcaatgttg ctaaaggtgg cgctttagag tgggttcccc aggagacgat
     1621 tttttataac cattccaatg tgaagataga tcatcaggtg gcactggagg atgacgcagt
     1681 ttatgttggt tgcgagatct tatgttttgg tcgaactgcc tatggagaaa catttgacga
     1741 gggtcagatt aagcaacgaa ctcgtataat ccggaatggg aaaatgattt ggctag
//
LOCUS       DAOQLJ010000283         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_53111_length_1694_cov_3.762660, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000283 DAOQLJ010000000
VERSION     DAOQLJ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53111_length_1694_cov_3.762660"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PK522_12075"
     CDS             complement(<1..194)
                     /locus_tag="PK522_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42513.1"
                     /translation="MFLFSERTGFASVNGVNNTMAQGSRIIATPAERLARISNVSEAH
                     FARSFKEAFGVPPHRYLLTRR"
     gene            481..1179
                     /locus_tag="PK522_12080"
     CDS             481..1179
                     /locus_tag="PK522_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNC42514.1"
                     /translation="MFIEKHTNDASLNLGFEWNFEQLEKEFPSIRLINLNDRSFLYHQ
                     GDKCSSLFLIKGGIVKLSHLTAQGNELTFALLRKGDIVGCLDIVNADHVMEENAQALG
                     KVDFYRIECNDFKQLITLRSDLSWSVLTSIYARKQQIERKLIATHTQPVKQRVIKMLF
                     ELAQLFGTRCLHGYALEIFLTQQELADLVGASRSVVSTIMNNFRDQGVLNYTREQICI
                     NDSALISIELSSES"
     gene            1276..1635
                     /locus_tag="PK522_12085"
     CDS             1276..1635
                     /locus_tag="PK522_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42515.1"
                     /translation="MKIISPRSHGYLDFLTVVIFALAPTLLDLSKVPAILSYGLAAAH
                     LVVTLISGFPTAALKLIPFIVQGWIERVVGPALIIAPFALSFSDEILASNFFIAMGVI
                     IIAVGFFTNYQGDNRSY"
BASE COUNT          524 a          317 c          324 g          529 t
ORIGIN      
        1 cggcgtgtca gcagatagcg atgtggtggt acaccaaagg cctctttgaa cgaccgtgca
       61 aaatgtgcct cagaaacatt actgatgcgc gccagccgct cagccggcgt cgcaataatt
      121 cgggaacctt gagccatcgt attatttacc ccgttaacag atgcaaaccc agttcgttca
      181 ctaaacagga acatataaag ccaacgtagt ggcgcagtat tacgaattgc agaaactgac
      241 gcaacaataa ctcatatgaa cgtataaaaa cgagaaataa aatttcgccc gcaagcgggc
      301 acctacaata gtttgtattt cactttacac tttacacttt acatataccg acatatatca
      361 attaatgtga cttccgtctt atgcgaagtt ttccaatctg ctactgataa agcgcaaaaa
      421 taacctaccg attattataa tgatcgcatt tctacatacc attgaggagc aaagtaatcg
      481 atgtttattg agaaacacac aaatgatgca tcgctgaatt tagggtttga atggaatttt
      541 gagcaacttg agaaagagtt tccttctatt cggttaatca accttaatga cagatctttt
      601 ctttatcacc aaggagataa atgctcaagt cttttcttga taaaaggtgg tatcgttaaa
      661 ctttctcatc taactgcaca aggaaatgag cttacttttg cgctcctaag aaaaggagat
      721 atagtcggat gtttggatat agtgaatgct gatcatgtta tggaagaaaa cgctcaggca
      781 ctcgggaagg ttgattttta tcgcattgaa tgtaatgact tcaagcaatt aataactttg
      841 cgaagtgatt tatcctggtc agttttaaca tcaatctatg ctcgcaagca acagatcgaa
      901 cgcaaattga ttgctactca tactcagcca gtcaagcaaa gggttatcaa aatgcttttt
      961 gaacttgctc aattatttgg aacacgatgt ctacatggat atgcgctgga aatttttctt
     1021 acccaacaag agctggcaga tctagtaggt gcaagtcgct ctgttgtaag tactattatg
     1081 aataatttta gagatcaggg agtattgaat tatacgagag aacaaatttg tattaatgac
     1141 tcagccttaa tatcaattga gctttcatct gagtcgtgac ttttttcatg gaaggtgttt
     1201 tctaaataac agacttgagt gaagcattct attaaagtac cacatatcat catctttaat
     1261 aaaaggaatg ctctaatgaa aataatttca cctcgtagtc atgggtactt agattttctt
     1321 acagttgtta tattcgcgct agctccaaca ctactggatt tgagcaaggt gcctgctata
     1381 ctgtcttacg gattagcagc agctcatctt gttgtgacac taatttccgg gtttcccact
     1441 gccgctctta agcttatacc atttattgta caaggttgga ttgagcgcgt tgttgggcca
     1501 gcattaatta ttgcaccttt tgccctgagt ttctccgacg agattttagc tagcaacttt
     1561 tttattgcaa tgggagttat tattattgct gtaggatttt tcaccaatta tcaaggtgat
     1621 aaccgctcat actagagtag aaaaaataat attttaagta gtaataagat gttaattatt
     1681 aaaaatataa tcgt
//
LOCUS       DAOQLJ010000284         1683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_53808_length_1683_cov_6.449017, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000284 DAOQLJ010000000
VERSION     DAOQLJ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53808_length_1683_cov_6.449017"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="PK522_12090"
     CDS             complement(<1..1085)
                     /locus_tag="PK522_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNC42516.1"
                     /translation="MSKCDTLTAKKILLVDDDTDLLELLSIRLSAAGFETITTNSAEA
                     AINCLDLTRPHLVISDMQMSGLDGMALFEHIHRTNPTLPVIILTAYGTIPDAVNAVQR
                     GIFGYLTKPYDPKTLLDQVDQALRLSCTNPVNDQTEASWRKTIITQSAEVEDLLTKVE
                     LVADGDASVLLNGESGVGKELFARAIHQASKRANQPFVTINCAAIPEQLLESELFGHV
                     KGAFTGAIRDHKGLFQLAEGGTLFLDEIGDMPLLLQVKLLHALQERVIRPVGSMQTVP
                     INVRIISATHRDLKEGIAEGVFREDLYYRLYVVGLTIPSLTQRREDIPLLANHFLRLF
                     ANKYEKNINGFAPEAINFLLSAAWPGNV"
     gene            complement(1082..1561)
                     /locus_tag="PK522_12095"
     CDS             complement(1082..1561)
                     /locus_tag="PK522_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42517.1"
                     /translation="MHPVQKAEIPVIYTEQTAPSPPSDIENLMRYYQALQDKKPFELQ
                     WEFTYAKHHYRNDKNAENRFKYILLVTLPNTNFRDTSLALNLLTNWPEDIPLSPNLIS
                     FRKILTTSLKDRQSAKYNNQSLLQKLKTSEGKIKALQDKIDAIKSMERQLFRGKIPQ"
BASE COUNT          492 a          308 c          343 g          540 t
ORIGIN      
        1 acattaccag gccaagcggc gctaagcaga aaattaattg cttcaggtgc aaatccatta
       61 atattttttt catatttatt cgcaaataac ctaagaaaat gattggcgag cagtggaata
      121 tcttcacgac gttgggttag cgaggggatc gttaagccga cgacatataa tcgataataa
      181 agatcttctc gaaacacgcc ttcggcaata ccttccttga gatcgcggtg tgtagcagag
      241 ataatccgta cattgatagg aacagtctgc atcgatccta caggtcgaat cactctttct
      301 tgcagggcat gtaataattt aacttgaaga aggaggggca tatctccaat ttcgtctaaa
      361 aataacgttc ccccttctgc taattgaaac aatcctttgt gatctctaat ggctcctgta
      421 aaggcacctt tgacgtgacc aaataattca gactcgagta attgttctgg aatagcagca
      481 cagttaattg ttacaaaagg ctgatttgcg cgctttgagg cttgatgaat cgctcgcgca
      541 aacagttctt ttccaacgcc actttcccca ttaagtaata cactcgcatc tccatctgcg
      601 acaagctcaa ctttcgtaag caaatcttca acttctgcac tttgggtaat aatcgttttt
      661 cgccatgaag cttcggtttg gtcgttaact ggattggtac atgatagtcg gagcgcttgg
      721 tcaacttgat caagtaaggt tttaggatcg taaggttttg taagatagcc aaaaataccc
      781 cgctgtacgg cattgacagc atctgggatg gtgccatagg cagttaaaat aataacgggt
      841 aaggtagggt ttgtacgatg aatgtgttca aataaagcca tgccatctaa tccactcatt
      901 tgcatatcgc tgatgacaag atgagggcga gtgaggtcta ggcaatttat tgcagcttca
      961 gcactattgg tggttattgt ttcaaatcca gcggctgata agcgtattga taataattca
     1021 agtagatcgg tatcatcatc aacaagtaag attttttttg cagttaacgt atcacattta
     1081 ctcattgggg tatctttcct cgaaataatt gtctttccat acttttaatt gcatcgattt
     1141 tatcttgtag cgctttgatt tttccttccg aagtttttaa tttttgtaaa aggctttggt
     1201 tattatattt cgcagattgc cggtccttaa gagaagtcgt taatattttt ctaaaactaa
     1261 ttaaattagg agatagtgga atatcctcag gccaattggt taataaattc aatgctaaag
     1321 aagtatctct gaaattggta ttgggtaaag taacaagcag aatgtattta aatcggtttt
     1381 ctgcattttt atcatttcga taatgatgtt ttgcataagt gaattcccat tgaagttcga
     1441 atggtttttt atcttgcagc gcttgatagt aacgcattaa attttcgata tcgctcggag
     1501 gagagggtgc agtttgttca gtataaatca cgggtatttc tgctttctga acaggatgca
     1561 ttgcacatcc ggttatagaa aaaataattg caaaatatac aaataactta aagttattta
     1621 ccaattatcg ttcctcaaga tctacaactg ggagcgttaa acgaaaatgg gctccgggct
     1681 cat
//
LOCUS       DAOQLJ010000285         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_54235_length_1676_cov_5.117829, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000285 DAOQLJ010000000
VERSION     DAOQLJ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54235_length_1676_cov_5.117829"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PK522_12100"
     CDS             complement(<1..848)
                     /locus_tag="PK522_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HNC42518.1"
                     /translation="MRREQLEENQIELYAVALAAGVGLGLWHPNFGASLEYLISPVLA
                     VLLYSMFTQIPFLQLRAAFANKRFTAALLIVNFIIVPIVVWFLSLLLPEHPPLLLGVY
                     LVLLTPCIDYVVVFTHLGRGNARLVLASTPLLLLTQMLLLPVYLWLFMEEQDAQVMSA
                     EPFLEAFLVLIVLPLGLALSTEFWAKRRRSGAIWLEVTAWLPVPFMAMTLLFVVASQI
                     DRIDEYLPVVAQVVPVYMAFMAIMPFLARLTAYAFRLDTQAGRALIFSAGTRNSLEVL
                     PLALALP"
     gene            complement(1010..1676)
                     /locus_tag="PK522_12105"
                     /pseudo
     CDS             complement(1010..1676)
                     /locus_tag="PK522_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215827.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          506 a          394 c          372 g          404 t
ORIGIN      
        1 ggcaaagcca atgctaaagg cagaacctcc agcgaattcc gcgtccctgc gctaaaaatg
       61 agtgctcgtc ctgcctgtgt gtccaggcga aacgcatatg ccgtcaagcg agcaagaaac
      121 ggcatgatag ccatgaaggc catataaact ggcacgactt gtgccacaac cggaaggtac
      181 tcgtctattc tgtcaatttg agatgctacg acgaaaagta atgtcattgc cataaagggc
      241 accggaagcc acgccgtgac ttcaagccag atggcaccgc tacgtcggcg ttttgcccaa
      301 aactcggtcg ataaggcaag tcccagagga agcacaatca atacaagaaa agcttccagg
      361 aatggttcag cactcatgac ttgtgcatct tgctcttcca tgaaaagcca tagatacacg
      421 ggcagaagaa gcatctgggt aagcagcagc aaaggtgtgg aagcaagcac gagtcgggca
      481 ttgccacgac caagatgggt aaaaacgaca acgtaatcaa tacaaggtgt taacagaacc
      541 agatataccc caagtagcag tggtgggtgc tccggtaaaa gaagcgacaa aaaccagacc
      601 acgatgggta cgatgatgaa attgactatc aatagcgcgg ctgtaaaccg tttgttcgcg
      661 aacgcagcgc gcaactgaag gaatggaatc tgcgtaaaca tgctataaag cagcaccgca
      721 agcacggggg aaatcaaata ctctaggctg gcaccaaagt taggatgcca caacccaagc
      781 cccaccccgg cagccaaggc taccgcgtat aactctatct gattttcttc cagctgttct
      841 cttcgcatgt gtttccttaa aatacaaacc atcccgggtc agcgccaaat ttgtgccata
      901 atttggcgca acgttttatt tcaatgattc tgatcatttt ggtataacga ttgtaattca
      961 acctataaat cttcgatcga ctccatataa ttttatggtt cttaggaagt taaagcgggt
     1021 tagaacgtag taatttaaga attgcataat agagattatc acgacgatga ttaaatcgaa
     1081 attctgtttc tttcaggtgt agataaaagg tatgttcagg aacgccattg aactttgcca
     1141 aacgtctttt tgcaaaaccc cagaaagatt cgattccgtt aatgtgccgt tcgccactgg
     1201 caaactagtt gttgccatga tgaaccctga agtgtttatc aaaaccgata tcgaccagcc
     1261 cgtcatagcc gtgccaccca tcggaatgaa tgattatgtc tggtgctaca tgcccacgga
     1321 taatggtttt caatgtggct ttagagcaat ccggaacaat ctccgtatat actttcccca
     1381 ggcgcttcaa tacaccaaaa acaattgttt tgccaaaaca cctcgacctc ttttacccct
     1441 gatgcgttgc gcaccaaaat aagactcgtc gacttctaca gcaccttgaa gaggcgattc
     1501 aagctcgcaa cactgagcta ttctttgccg gactttgagg taaatcgtat taacagaccg
     1561 tgtagaaata tcggtcaatt gtgcggtact tgtggcagta aaatccagag caaaacagcg
     1621 gataagttgc ctgaatttag cttctctgat tcgagaacga aaatagtatc tgtttt
//
LOCUS       DAOQLJ010000286         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_56114_length_1647_cov_7.167714, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000286 DAOQLJ010000000
VERSION     DAOQLJ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56114_length_1647_cov_7.167714"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /gene="proC"
                     /locus_tag="PK522_12110"
     CDS             complement(<1..724)
                     /gene="proC"
                     /locus_tag="PK522_12110"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNC42519.1"
                     /translation="MKITFIGGGNMASALIGGLLQQNFPMTQIHVVEINAETANRINQ
                     QFGVGTTTDLIEGISGSNVIVLAIKPQQLHGVAQALKPLLTSQLVISIAAGVRTRDLS
                     RWLGNYQYIVRAMPNTPALVRAGMTGLYAIPSVSVALRDDATTILGAVGSVLWLEEED
                     QLDAVTAVSGSGPAYLFYFMEAMEQAGVELGLTLDQARELTIHTFIGASQLAYQSHED
                     VAVLRSRVTSKGGTTEQALLSMS"
     gene            complement(827..1033)
                     /locus_tag="PK522_12115"
     CDS             complement(827..1033)
                     /locus_tag="PK522_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HNC42520.1"
                     /translation="MGRWLIIIGALLFFLGVIVQFAPWLISWFGKLPGDIRIESERGK
                     IFIPITSMLIISIVITLLINLFKR"
     gene            complement(1107..>1647)
                     /gene="mutM"
                     /locus_tag="PK522_12120"
     CDS             complement(1107..>1647)
                     /gene="mutM"
                     /locus_tag="PK522_12120"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNC42521.1"
                     /translation="FDLKVSSGAVLRYRDPRRFGAILWSDAEKIAQHPLLVNLGVEPL
                     SDTFDGKMLYERSRHRNLSVKVFLMNHQIVVGIGNIYANEALFHAGINPTVLAKRINL
                     KRYEKLADAIKTTLNAAIEAGGSSLRDFVNSEGKPGYFQQHYWVYGRDNQPCKRCGQL
                     ITKIKQGQRSSFYCRHCQK"
BASE COUNT          486 a          398 c          286 g          477 t
ORIGIN      
        1 ggctcatact gagcaatgct tgctccgtcg tccccccttt agaagtgacg cgtgatcgta
       61 acaccgcaac atcctcatga ctttgatatg caagctggct ggcgccaata aatgtgtgaa
      121 tcgtcaattc ccgagcttgg tcaagcgtta aacctaattc gacgcccgct tgctccattg
      181 cttccataaa atagaacaga taagctgggc cacttccaga aactgcagtt actgcatcca
      241 attgatcttc ctcttctaac cacaaaacag agcctaccgc gcccaaaatt gtagttgcat
      301 catccctcaa cgcgactgaa acgctgggta ttgcatataa acccgtcatt ccagctctta
      361 ctaaagcggg tgtattcggc atggctctca caatatactg ataattgccc aaccaccgtg
      421 acaaatcccg agttcgaaca cctgccgcaa tcgaaataac caactggctg gttaacaacg
      481 gttttagcgc ctgagcaacc ccatgtagct gttgtggctt aattgccaat acgatcacat
      541 tgctgcctga aataccttca atcaagtctg ttgttgtacc aacgccaaat tgctgattaa
      601 ttctgttagc tgtctcggca ttgatttcga cgacatggat ttgtgtcatt ggaaagtttt
      661 gctggagtaa tccgcctatc aatgcacttg ccatattgcc accaccaata aaggtaattt
      721 tcatgtttca cctatatgtt tatatcgttt attcatggct tgggtagtct atttcataat
      781 taaataaaga ttaagtcgat taaagaagaa aattgcctca attcatctag cgtttgaata
      841 aatttataag caaggtaatt acgatgctga taattaacat ggaagtaatt ggaataaaaa
      901 tttttcctct ttcggattca attctgatat cccctggcaa ttttccaaac caactgatta
      961 accaaggtgc aaattgaaca atcacaccca gaaaaaataa taatgctccg ataataatta
     1021 accaacgacc catcacaagc atcccacacg gctcaattac ttacctcttt gattgataag
     1081 cgattcaatt tataaatttg atttgtttac ttctggcaat gacgacagta aaaactagac
     1141 cgctgtccct gcttgatttt agtaattaat tgcccacagc gcttacacgg ttgattgtct
     1201 cggccataaa cccagtaatg ctgctgaaaa taacctggct tcccttcact attcacaaaa
     1261 tcacgtaggc tactgccacc ggcttcgata gcagcattga gtgtcgtttt aatcgcgtct
     1321 gctaatttct cataacgttt tagattaata cgttttgcta aaacagtcgg atttatcccc
     1381 gcatgaaata atgcttcatt tgcgtagata ttcccaatac caaccacgat ctgatgattc
     1441 atcagaaata ctttaacact gagattccga tgacgagaac gttcatatag catcttgcca
     1501 tcaaaagtat cactcaacgg ttctactccg agattaacca atagtggatg ctgtgctatt
     1561 ttctctgcat cgctccataa gattgcccca aaacggcgag gatctcggta acgcaacaca
     1621 gcgccactac tcactttaag atcaaat
//
LOCUS       DAOQLJ010000287         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_59260_length_1600_cov_7.339159, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000287 DAOQLJ010000000
VERSION     DAOQLJ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59260_length_1600_cov_7.339159"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /gene="purT"
                     /locus_tag="PK522_12125"
     CDS             <1..1096
                     /gene="purT"
                     /locus_tag="PK522_12125"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HNC42522.1"
                     /translation="ETIAVDRYANAPGHQVAHRAHVINMTDPMALRSLIEQERPAIIV
                     PEIEAIATEVLLEIEKEGLATVIPTARAARLTMNREGIRSLAAETLGMPTSPYAFANS
                     LAALRAAIDEQIGYPCIVKPVMSSSGKGQSRIENVDGIEAAWNYAASGGRVDQGRVIV
                     EGVIDFDYEITQLTVRAMNDRAEVQTYFCEPIGHVQVNGDYAESWQPQAMTAIAMERA
                     RELAKKITDNLGGLGIFGVELFVKGDEVWFSEVSPRPHDTGMVTMCSQKQSEFDLHAR
                     AILGLPVDTTLSAPGASAVIYGQLEEKGIAFTGLGEALSVPQSDIRLFGKPESFKKRR
                     MGVALANGANTDEARTRAKLAASRVCPIKS"
     gene            1194..1559
                     /locus_tag="PK522_12130"
     CDS             1194..1559
                     /locus_tag="PK522_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC42523.1"
                     /translation="MSQFVIAFLGLFFITTLTYSQESADEKRLDEIAERGAHVMPFDL
                     EKTLHIFSKTNHGGIQQVIVKDKSDTEQINLIRSHLSKIAAEFKRSNFSNPEKIHGKN
                     MPGLADLKTAKDGQIKICL"
BASE COUNT          475 a          297 c          402 g          426 t
ORIGIN      
        1 tgaaaccatt gctgtcgatc gttatgcaaa tgcacccggc catcaagttg cgcatcgtgc
       61 acatgtcatc aatatgactg acccaatggc attacgcagt ttaattgagc aagagcgacc
      121 cgctatcatc gtacccgaaa tcgaggcaat tgcgacggaa gtattactag agattgaaaa
      181 ggaagggctt gcaacggtta ttccaacggc acgcgcagca cgattgacta tgaatcgtga
      241 aggtattcgt agtttagctg cagaaacgtt aggcatgcca acttcacctt atgcatttgc
      301 aaatagccta gcggcgttgc gagctgcaat cgatgaacaa attggttacc cctgtatcgt
      361 taaaccggtt atgtcttctt cgggtaaggg gcaatcacga atcgaaaatg tggatggaat
      421 tgaagcggct tggaattatg cagctagcgg cggacgagtc gatcagggac gtgtcattgt
      481 ggaaggggtc attgattttg attacgaaat aacacaatta accgtgcgag caatgaatga
      541 tcgtgctgaa gtacaaacct atttttgtga accgattggt catgtccaag tgaatgggga
      601 ttatgctgaa agttggcagc ctcaggccat gacagcgatt gcgatggagc gagcccgtga
      661 attggctaag aaaattacag ataatttggg aggcctcggt atttttgggg tagaactgtt
      721 tgttaaaggt gatgaggtat ggtttagcga agttagtcct cgccctcatg atacggggat
      781 ggtgacgatg tgtagtcaga agcaaagcga gtttgattta catgcgaggg caatcttggg
      841 tttgccagtt gatacgacac tttcagcacc cggcgcgagc gcagtgattt atggtcaact
      901 ggaagagaaa ggtatagctt ttacaggcct tggagaagca ttgagtgttc cacaaagcga
      961 tatacggtta tttggtaagc cagaatcctt taagaaacga cggatggggg ttgcgttagc
     1021 caacggtgcc aacaccgatg aggcgcgaac ccgtgctaaa ttggcagcga gccgagtttg
     1081 cccgattaaa agctaagaat tacggcttgt ttcagcttta tttccagaaa aattgcactt
     1141 gcgaaaaagt tgatatgaat tctaaagaga tctcttaata aggagagact aaaatgagcc
     1201 aattcgttat tgcttttctg ggattatttt tcattacaac cctaacctac agtcaagagt
     1261 cagcagatga aaagcggctc gatgaaattg cagagcgtgg tgcgcacgtc atgccatttg
     1321 atctagagaa aacactacat attttttcta aaacgaatca tggagggatt cagcaggtta
     1381 ttgtgaaaga taaatctgat actgagcaaa ttaatttgat tcgatcgcac ttatctaaaa
     1441 ttgcagcaga atttaaacga agtaattttt ctaatccaga aaagattcat ggcaagaata
     1501 tgcctgggct tgctgatttg aaaactgcca aagatggtca aataaaaata tgcctctaaa
     1561 ctattttcag tttaatctgg gtgtgactca aacaggcatt
//
LOCUS       DAOQLJ010000288         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_59261_length_1600_cov_6.384466, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000288 DAOQLJ010000000
VERSION     DAOQLJ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59261_length_1600_cov_6.384466"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /gene="fur"
                     /locus_tag="PK522_12135"
     CDS             complement(<1..379)
                     /gene="fur"
                     /locus_tag="PK522_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HNC42524.1"
                     /translation="MSKEDLKSRDLKTIGLKTTLPRLKILNLFENSAIRHLSAEDIYK
                     ELLNEGEDIGLATVYRVLTQFEQAGLLERHHFEGGKAVFELTSDKHHDHLVCLQCGRV
                     EEFYDADIEKRQIKIAKDRGFSLQ"
     gene            453..926
                     /gene="bamE"
                     /locus_tag="PK522_12140"
     CDS             453..926
                     /gene="bamE"
                     /locus_tag="PK522_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNC42525.1"
                     /translation="MHVKFLLLSIVSLSISCSYLPSLPYKIDIQQGNVVTEDMVEKLR
                     LGMTRSQVRFVLGSPLITDVFHINRWDYVYSLAPSGRLSEKRRLSIFFDGDLLTHIEG
                     DFKLPILPANDLPLPSPNSTDEVPEDDEKKNEDSEELQPKEKKEDKKGTTDMIAP"
     gene            954..>1600
                     /gene="dapB"
                     /locus_tag="PK522_12145"
     CDS             954..>1600
                     /gene="dapB"
                     /locus_tag="PK522_12145"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNC42526.1"
                     /translation="MLNIAIAGSTGRMGRTLLETIAESPHMRLSAALEQTNSPFLGKD
                     AGELIGAPCDVSINADIAAALSGSDVLIDFTRPAGTLAHLAICRKQGIKMVIGTTGFT
                     ADEKDILKSAANEIAMVFAPNMSVGVNILFKLLEVAAHALRDGYDIEIVEAHHRHKVD
                     APSGTALRMGEVIAQALDRDLSKVAVYGREGVTGERLPETIGFSTIRGGDIVGDHT"
BASE COUNT          465 a          320 c          344 g          471 t
ORIGIN      
        1 cttgcaagct aaatccacga tctttggcga tcttgatttg gcgcttttca atatcagcat
       61 cgtaaaattc ctcgacacgg ccacactgca ggcaaaccaa atgatcgtgg tgcttatcgc
      121 tggttaattc gaaaactgct ttaccgcctt caaaatggtg cctttccaat aaacccgctt
      181 gctcaaactg agtaagaacg cggtaaaccg ttgcaagacc aatatcttca ccttcgttaa
      241 gtaattcttt ataaatatct tcagctgaca agtgacggat agcactattc tcaaacaaat
      301 ttaatatttt caaacggggt aaggtagtct taagccctat tgttttgagg tctctacttt
      361 tgagatcttc tttactcatg gtttaatcag tcctaaaagt tatttgctgt atcatatagc
      421 gcttggttag ctttgaaaac tctcgtttga gtatgcatgt aaaatttttg ttgcttagta
      481 ttgtatcgct atcgattagc tgctcttatc ttccatcact tccctacaaa attgacattc
      541 aacaaggtaa tgtcgtaact gaggatatgg tagaaaagct gcgactgggt atgactcgat
      601 cccaagtacg ttttgtactg gggtcaccgc tcattactga tgtttttcat attaatcgat
      661 gggattacgt gtatagcttg gcccctagcg ggaggctatc agaaaaacgc cgtttatcca
      721 tttttttcga tggtgattta ttgacgcata ttgaaggtga ttttaagctc ccaattttac
      781 cggctaatga tcttccattg ccttcaccta attccactga tgaagttcct gaagatgatg
      841 aaaagaaaaa tgaggattct gaagaattac agccaaaaga aaagaaggaa gataaaaaag
      901 gtacaacaga tatgatagct ccttaaaact attctcataa tgaatcaaaa tctatgttaa
      961 atatagcaat agcaggtagt actggtcgta tggggcgaac attactggaa accatagcgg
     1021 aatcacctca tatgcgttta tccgcagcgt tagagcaaac gaatagcccc tttctcggaa
     1081 aagatgctgg tgaattaatt ggagctcctt gcgatgtttc tattaatgca gatatcgcag
     1141 cagcgttatc cggcagcgat gttttaatcg atttcacgcg tccagcaggt acgctagcgc
     1201 atctagctat ttgtcgcaag caaggtatca aaatggtcat tggtaccaca gggtttacag
     1261 ccgacgagaa agatatactt aaatcggctg ctaatgaaat tgcgatggtt tttgcgccta
     1321 atatgagtgt aggggtaaac attctattca agttactcga agttgccgcg cacgcattaa
     1381 gggatggcta cgatatcgaa attgtagaag cgcatcatcg tcataaagtc gatgcgcctt
     1441 ctggaacagc gctgcggatg ggagaagtaa tcgcccaagc cttagatcgc gatttatcaa
     1501 aagttgctgt ctatggaaga gaaggtgtga caggcgaacg attaccagaa actattgggt
     1561 tttccacgat acgcggcgga gacatcgttg gcgatcatac
//
LOCUS       DAOQLJ010000289         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrosomonas sp. isolate AMR_MDS_0551
            NODE_64131_length_1537_cov_5.386640, whole genome shotgun sequence.
ACCESSION   DAOQLJ010000289 DAOQLJ010000000
VERSION     DAOQLJ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810183
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosomonas sp. (wastewater metagenome)
  ORGANISM  Nitrosomonas sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosomonas.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:59:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,400
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 5 of 14
            Pseudo Genes (incomplete)         :: 8 of 14
            Pseudo Genes (internal stop)      :: 5 of 14
            Pseudo Genes (multiple problems)  :: 3 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Nitrosomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64131_length_1537_cov_5.386640"
                     /isolate="AMR_MDS_0551"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:42353"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            647..1012
                     /locus_tag="PK522_12150"
     CDS             647..1012
                     /locus_tag="PK522_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC42527.1"
                     /translation="MYNLILITNILRILDEKDMTKSELAEKAGVSISFFTDLTNDKAN
                     PSLRIIEAVAEALETPLPMLLDSSDMSAADLEALTNRKLKHLPKGFVWKGGVLSEFEA
                     YQVEQWDKKNRAFLLKNKK"
     gene            1109..>1537
                     /locus_tag="PK522_12155"
     CDS             1109..>1537
                     /locus_tag="PK522_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011013261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HNC42528.1"
                     /translation="MTSQDQLSHITTVKDRAKSKLERDARGILSALQDPETVEIMVNA
                     DGRIWQEKLGQKIQHIGNIQAAQVEAVIKTVAGFHGKEINRFNPIIEGEFPLDNSRFA
                     GQLPPIVSSPTFAIRKKAIKIFTLEQYVETGVLSPRHSDVI"
BASE COUNT          475 a          301 c          296 g          465 t
ORIGIN      
        1 tgactccgcg taatttaatc gtctccgtta attctcgtaa cgtttcctga ttaaattcct
       61 tcctgggttg atgaatatct tcgctgatca gcgtgatgtc aatttctgtc gcgccgatag
      121 gcgcaacaag ccctttttta ttatcctttt ttcttcctgt catttttatc gtgggcctaa
      181 cttgcgctcg ttatactggg tggaaagata gaaactatgg atgcgattat caataaataa
      241 aaccgcgcaa aaaaattatc tttgcacttc ggtgtttttg ttcttcttaa tcagtttatt
      301 attgaatgct tgatcctcgt gtcagaaaac ggcttacgtt ttcagattct caagattttc
      361 tattagttac tcatctaccc tctattcata gcattctaag gtaactatca atatttatta
      421 tatttataca aatcaacgat gtaagtaatt attgatttga attctatttt aatcctatta
      481 ttaataatta tccacaaact atgtatataa cgaactatta tttggccctc cattgaatta
      541 atgaatttgt atttttattt tggattaaaa tagcagcaaa actgctgcaa aacaatggtg
      601 ctgattagtt aagctgctta aaccctaaaa atggcagggc tattatatgt acaatttaat
      661 cttaatcaca aatattttgc gtatccttga tgaaaaggat atgaccaagt cagaattagc
      721 agagaaggcg ggtgtttcca tctctttttt tactgatctg accaatgaca aagccaatcc
      781 atcactcagg attatagaag ccgtcgcaga agcactagag accccactac caatgctact
      841 ggatagttcg gatatgagcg cggctgatct tgaagcatta accaatcgaa aactaaagca
      901 tttgcctaaa ggctttgtct ggaaaggggg cgtgctgagt gaatttgagg cttatcaagt
      961 cgagcaatgg gataaaaaga acagagcgtt cttattaaaa aataaaaagt aagcaaaaga
     1021 gaaaatagtt aaaagcagta aaaaagtatt gcgcaaaata taattacgtt atattgtaat
     1081 gtcgtgtgta agctgttgga gtactcgcat gacatcgcaa gatcaattat ctcacatcac
     1141 aaccgttaaa gaccgggcaa agagcaagct ggagcgcgac gcacggggaa tactgtccgc
     1201 actgcaagat cctgagacgg ttgaaatcat ggtcaatgcc gatggccgta tctggcaaga
     1261 gaagctgggt caaaagatac aacatatcgg aaatatccag gctgcccagg ttgaagctgt
     1321 catcaaaacg gtagcgggtt tccacggcaa ggaaatcaat cgattcaatc caatcattga
     1381 aggtgagttt ccactcgata actcacgctt cgccggtcaa ttacctccca ttgtttcatc
     1441 ccccacattt gcaattcgta aaaaagccat caagattttt acgcttgagc agtatgtaga
     1501 aaccggtgtg ttgtcgccca gacactctga tgtcatc
//
